LOCUS       DAWFHY010000001        61301 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.001, whole genome shotgun sequence.
ACCESSION   DAWFHY010000001 DAWFHY010000000
VERSION     DAWFHY010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 61301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.001"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(130..606)
                     /locus_tag="WGQ68_000001"
     CDS             complement(130..606)
                     /locus_tag="WGQ68_000001"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEZ0713899.1"
                     /translation="MLHLLKSLKPSGKGAIILPHGVLFRGNAEARIRTELLNLDLIKG
                     IIGLPANLFYGTGIPACIIVIDKEHAQTAQFAEEGTNQVISGGSVFMIDASRGFIKDG
                     NKNRLREQDIHKIIDTFTKQEEIPRYSRMVHLSEIAAQDYNLNLPRYIDSGEVEDL"
     gene            complement(494..850)
                     /locus_tag="WGQ68_000002"
     CDS             complement(494..850)
                     /locus_tag="WGQ68_000002"
                     /inference="COORDINATES: protein motif:HMM:NF014442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0713900.1"
                     /translation="MLKAAAQAGSQISLYGQEKDVATASLARMNMILHNNETAEINTG
                     NTLSDSSFRDENDGLKTFDFAVANPPYPARKKRRLRLFAASAQKPETKRQRCDYSSAR
                     CAVSRQCRSAYSHGIA"
     gene            complement(763..1410)
                     /locus_tag="WGQ68_000003"
     CDS             complement(763..1410)
                     /locus_tag="WGQ68_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0713901.1"
                     /translation="MHHFGRAATSCAAVWMRAFIKNYVLTLLFLKYVSDKHKYGGGMI
                     ELHAGTTFDDIVKLKNTADIGDRLNKIIAQIAEANDLKGVINVTDFNDEDKLGKGKEM
                     IDRLSRLVGIFKKLNLSSNQAEDDDLLGDAYEYLMRHFATESGKSKGQFYTPAEVSRI
                     MAKIIGISADCRPAPAFMTRPAARVRCCSKPPPKPAAKSAFTVRKKMWQPRPLPV"
     gene            complement(1307..1516)
                     /locus_tag="WGQ68_000004"
     CDS             complement(1307..1516)
                     /locus_tag="WGQ68_000004"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0713902.1"
                     /translation="MNADIAVAAGVLKSRLICNFHLLGKTLWQSKKNQLYASLWAGCD
                     ELRGGMDASLYKELCAYAIVFKICF"
     gene            complement(1513..2172)
                     /gene="ung"
                     /locus_tag="WGQ68_000005"
     CDS             complement(1513..2172)
                     /gene="ung"
                     /locus_tag="WGQ68_000005"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEZ0713903.1"
                     /translation="MDTWHDALGGEKQQPYFQEILNAVRQERLSGQIIYPPAADVFNA
                     FRLTAFDRVKAVILGQDPYHGAGQAHGLAFSVRQGIRIPPSLLNIYKELETDIEGFSI
                     PAHGCLTAWAEQGVLLLNTVLTVRAGQAHSHALLGWERFTDTVIRQLAAHRKHLVFML
                     WGGYAQQKRKLIDSQNHLILTAPHPSPLSAYRGFFGCRHFSQANSYLSRHGIDPINWK
                     L"
     gene            2253..2660
                     /locus_tag="WGQ68_000006"
     CDS             2253..2660
                     /locus_tag="WGQ68_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEZ0713904.1"
                     /translation="MTVWFVAAVAVLIIELLTGTVYLLVVSAALAGSGIAYGLTGSTP
                     AAVLTAALLSALGIWFVHAKTAVGKVETDSYQDLDAGQYAEILRHVGGNRYEVFYRGT
                     HWQAQNTGQEELEPGTRALIVRKEGNLLIITHP"
     gene            2675..3622
                     /locus_tag="WGQ68_000007"
     CDS             2675..3622
                     /locus_tag="WGQ68_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraslipin"
                     /protein_id="HEZ0713905.1"
                     /translation="MEFFIILLVAVAVFGFKSFVVIPQQEVHVVERLGRFHRALTAGL
                     NILIPFIDRVAYRHSLKEIPLDVPSQVCITRDNTQLTVDGIIYFQVTDPKLASYGSSN
                     YIMAITQLAQTTLRSVIGRMELDKTFEERDEINSTVVAALDEAAGAWGVKVLRYEIKD
                     LVPPQEILRSMQAQITAEREKRARIAESEGRKIEQINLASGQREAEIQQSEGEAQAAV
                     NASNAEKIARINRAKGEAESLRLVAEANAEAIRQIAAALQTQGGADAVNLKIAEQYVA
                     AFNNLAKESNTLIMPANVADIGSLISAGMKIIDSSKTAK"
     gene            complement(3818..4888)
                     /locus_tag="WGQ68_000008"
     CDS             complement(3818..4888)
                     /locus_tag="WGQ68_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEZ0713906.1"
                     /translation="MYRRKGRGIKPWMGAGAAFAALVWLVFALGDTLTPFAVAAVLAY
                     VLDPLVEWLQKKGLNRASASMSVMVFSLILLLALLLIIVPMLVGQFNNLASRLPQLIG
                     FMQNTLLPWLKNTIGGYMEIDQASIIAWLQAHTGELSNALKAWFPVLMRQGGNIVSSI
                     GNLLLLPLLLYYFLLDWQRWSCGIAKLVPRRFAGAYTRITGNLNEVLGEFLRGQLLVM
                     LIMGLVYGLGLVLVGLDSGFAIGMVAGILVFVPYLGAFTGLLLATVAALLQFGSWNGI
                     LSVWAVFAVGQFLESFFITPKIVGDRIGLSPFWVIFSLMAFGQLMGFVGMLAGLPLAA
                     VTLVLLREGVQKYFAGSFYRGR"
     gene            5073..5348
                     /locus_tag="WGQ68_000009"
     CDS             5073..5348
                     /locus_tag="WGQ68_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HEZ0713907.1"
                     /translation="MTEQKNKTSLIEFPCTFPLKVMGAVHPEFEQAVLDTVRLHAPDT
                     QAHHITTRPSSKGNYTGATVQVKVENQEQLDNIYRALTSHELVKVVL"
     gene            5351..5974
                     /gene="lipB"
                     /locus_tag="WGQ68_000010"
     CDS             5351..5974
                     /gene="lipB"
                     /locus_tag="WGQ68_000010"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HEZ0713908.1"
                     /translation="MKIIHKGLVEYLPTFEAMKTFNAGRNADTEDELWVVEHPPVFTQ
                     GLAGKPEHLLIRDDIPVVQIDRGGQITYHGPGQLVVYTMIDFKRRKTSVRNIVSALEN
                     SIIATLAEYGIEAAADPKRPGVYVGERKIASLGLRIKNGSVYHGLALNVNMDLSPFTH
                     INPCGYAGMEMTQIADFVQPCPTPDEVAAKLTAHLETQFTPKADNNE"
     gene            5967..6935
                     /gene="lipA"
                     /locus_tag="WGQ68_000011"
     CDS             5967..6935
                     /gene="lipA"
                     /locus_tag="WGQ68_000011"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEZ0713909.1"
                     /translation="MSEIKTDDPKRGIKLRGADKTARIPIKVVPLQEKLKKPEWIRAK
                     LPSRKFFEIKDILREQKMHTVCEEASCPNIGECFSKGTATFMIMGDICTRRCPFCDVG
                     HGRPNMLDPDEPRNLAESVKAMNLRYVVITSVDRDDLRDGGAQHFADCIKAIRETSPN
                     TKIEILVPDFRGRLDIALKILAETPPDVMNHNLETHPSLYRKARPGANYQHSLDLLKR
                     YKEMMPHIPTKSGIMVGLGETDEDVREIMRDMRAHNIEMITIGQYLQPSDGHLPVLRY
                     VTPEQFKIFEKEAYELGFSNAAIGAMVRSSYHADEQAAEALRECEF"
     gene            6998..8740
                     /locus_tag="WGQ68_000012"
     CDS             6998..8740
                     /locus_tag="WGQ68_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713910.1"
                     /translation="MDQQPVIEIAEFPHDNKLWRIDGLGKIFSQLKNNNNLFIEVYLR
                     PVNFHDNSVYNFAKFDSSGREIPEYNQLKKIPVNIGLLPILRVGSLWIKGKLQNNNAV
                     TDIATLKNIEINANTVEIINSNYKIKYLDSSGREQECKLLLQKNFATDAKVPFSCFKI
                     QNENYKNGIIIPVIEIIRFYYACSTRLAHLTFSNPDEITKISYAIDNETDKVVIKLPQ
                     QYTDQEAFILARILTSNIALSGLFQIKNSLIKIGVNEQPTTLLSTNFPFINPTNLMVR
                     GIKIVDRFLVTEICSCSAPFPDISVDRNNDNRKGDKKTDIPDDKKKEYRRGGQTPSLK
                     GNSTIQNQYESVNCVSTLQFNLNINCFSDLIGKKIEKPQKPFNEYKAQKLAINEKINV
                     TSLGVDLGTWSQTTIVPASISTFNTQSEKQNRDALAATFDNSVRAIEHLNEEYSDIQA
                     TIYMSSSRACEFVPLTDSAHKAQWSYLVSKTKTRRKVLLGKIEYKDKTFWLIEIQARK
                     SEHFVTAIIYFEAIDETKYLTQLLYVLAKQRGIWDKVPKNFSLQIITLKHTHKDIKSY
                     AQKIHKKISNFILK"
     gene            9012..10136
                     /locus_tag="WGQ68_000013"
     CDS             9012..10136
                     /locus_tag="WGQ68_000013"
                     /inference="COORDINATES: protein motif:HMM:NF015210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEZ0713911.1"
                     /translation="MKNRDTPHKKFINPTEYSLFAEEEQYDFELMIENQIKEHEKEID
                     YYVTDYPIETLVAKLDRQELFIPDYQRKNIWEKHRKSRFIESVLIGLPIPFLFFWQDP
                     STGNMEVVDGAQRLTALSEFIHGNLKLSYLEKLIYLNGQTYHQLLESRQKIFKNKPIR
                     GVVLSNKTDKEARLDLFERLNTGSKNATPAEIRRGLLQGAFYDMVETLSEDKTFNELA
                     KISKNMQDMGEREEFITRFFAYGEYLDHYSANVRKFIFDYTEIMNEEFAKNPNMRATY
                     EQKFHEIMAFFKEKYPIGFKRDGKGQSSRTRFEGMAVSAYLAEKENPGILAQVTPKEC
                     VDIFDSKEFIEVTGSDGANATKKLHARLFFAKNAFKRNHP"
     gene            10133..10885
                     /locus_tag="WGQ68_000014"
     CDS             10133..10885
                     /locus_tag="WGQ68_000014"
                     /inference="COORDINATES: protein motif:HMM:NF037890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713912.1"
                     /translation="MNSVKSMMESREEEINLYMDLLKYIHDSTQHGLPALQNRNGVEY
                     QLENTHLHILTASIYMLLYNYIEAVVTQSLNTLICYIQNERMPITQFSEPIQKEWLIG
                     GLNLNDAKASVETRLGNGLKMLKHFGDTQKIDIKISKGGGNFNDDEIFKLAKKLQVNM
                     CIDDETHRQLRQAGNITYESTRKFQDTCMKEITRERNKLAHGEITFQECGRNRTISDL
                     MAIKDTTFRYMHLFTDAFTEYLMQKKYLKPTV"
     gene            complement(10869..11891)
                     /locus_tag="WGQ68_000015"
     CDS             complement(10869..11891)
                     /locus_tag="WGQ68_000015"
                     /inference="COORDINATES: protein motif:HMM:NF012372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0713913.1"
                     /translation="MKIEAVDLFCGAGGLTAGLLRAGIKVKAGYDIDAACAYAYEYNN
                     KGAKFILQDVETVTGDQIQAHYSKDAVRLLAGCAPCQPFSTYNQGRDVRNDKKWPLLY
                     AFSRLIHEVQPELVTMENVPDVVKHEVYQDFVNNLKQQGYQVWAEKVLCADYGVPQSR
                     RRHVLLASRIGNIQMVPPTHSNCHVTVRQAIGHLPPLAAGKQDTIDVLHRCSSMSARN
                     LERIRASRPGGTWMDWPEDLRAACHRKKKGQSYKRIYGRMEWDKPAPTMTTLCIGFGN
                     GRFGHPEQDRAISLREAALFQSFPLDYQFTQETDTPIRQISRMIGNAVPVLLGEAIGN
                     SFKLLV"
     gene            complement(12325..13197)
                     /locus_tag="WGQ68_000016"
     CDS             complement(12325..13197)
                     /locus_tag="WGQ68_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HEZ0713914.1"
                     /translation="MNYPHLSPKAKEQLKYSDIERIEYIRSPRWIGYPQAQQVLVKLE
                     DLLSFPKQNRMPNMLLVGDTNNGKTMLIKHFLRQHPAHDNPHGNGIIAPVVIIQAPPI
                     PDESRFYNAILDLLFAPYKTHDRTDKKLAQVLHLLKYVNTKMLIIDEIHHILSGSLNK
                     QKTFLNVIKYLGNELQIPNPDELFTSWLVRLAHANGLKVQTFCHLLFPNYEIWNRDID
                     RHTPDWLVNTLAEKTGCSTIRIKTTTLDSLQGRLFDKNNFSGQLTWINSLKANHRKRD
                     NNAIVYCPLCLATD"
     gene            complement(13194..14519)
                     /locus_tag="WGQ68_000017"
                     /pseudo
     CDS             complement(13194..14519)
                     /locus_tag="WGQ68_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687573.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(14532..14801)
                     /locus_tag="WGQ68_000018"
                     /pseudo
     CDS             complement(14532..14801)
                     /locus_tag="WGQ68_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448901.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14804..15343)
                     /locus_tag="WGQ68_000019"
                     /pseudo
     CDS             complement(14804..15343)
                     /locus_tag="WGQ68_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714285.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(15340..15546)
                     /locus_tag="WGQ68_000020"
                     /pseudo
     CDS             complement(15340..15546)
                     /locus_tag="WGQ68_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease C-terminal domain-containing
                     protein"
     gene            complement(15560..15979)
                     /locus_tag="WGQ68_000021"
     CDS             complement(15560..15979)
                     /locus_tag="WGQ68_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniB family NTP-binding protein"
                     /protein_id="HEZ0713915.1"
                     /translation="MYAFYCHINKMAYLANTAKHIVGVGIKDAYRAIQTDAQLANRFE
                     PAVLPRWELNKDFMRLLMSFERMLPLSQPSDLHEISLATKLYGMSEGYIGELSRLLTQ
                     AAVAAIQQQTECINETILNSINWISPSQRKRQLDKLR"
     gene            complement(15900..16370)
                     /locus_tag="WGQ68_000022"
     CDS             complement(15900..16370)
                     /locus_tag="WGQ68_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn7 transposase TnsA N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0713916.1"
                     /translation="MPIRKIPKNYRNITGIAPYNKAIGIAAYESSLERDFLTLLEFDS
                     NVQHFEVQPITIEWFDPAGKKHIYTPDVLVHYQNDVIIYEVKYRSDLRKNWCELKPKF
                     QAALHFCKLHGWHFKLITELEIHTDYLCNVRFLLPYQQNGLSGEYSEAYCRCRY"
     gene            16582..16740
                     /locus_tag="WGQ68_000023"
     CDS             16582..16740
                     /locus_tag="WGQ68_000023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713917.1"
                     /translation="MEFTAKATAVAGIIKPGTPRQSEERFPAPAAPFDVNNINLNCPC
                     RLKALQTA"
     gene            17093..17557
                     /gene="bfr"
                     /locus_tag="WGQ68_000024"
     CDS             17093..17557
                     /gene="bfr"
                     /locus_tag="WGQ68_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0713918.1"
                     /translation="MQGNQAVLDYMNELLSGELAARDQYFIHSRLYSEWGYTKLFERL
                     NHEMEEETTHAEDFIRRILMLGGTPKMVRAELNIGTDVVSCLKADLQTEYEVRDALKK
                     GIKLCEEAQDYVTRDLMVAQLKDTEEDHAHWLEQQLRLIELIGEGNYYQSQL"
     gene            17585..18058
                     /gene="bfr"
                     /locus_tag="WGQ68_000025"
     CDS             17585..18058
                     /gene="bfr"
                     /locus_tag="WGQ68_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEZ0713919.1"
                     /translation="MKGDRLVIRELNKNLGLLLITINQYFLHARILKNWGFKELGEHF
                     FKQSIVEMKAADDLIERILFLEGLPNLQELGKLLIGESTEEIIACDLTKEQEKHEALL
                     AAIATAEAQQDYVSRDLLEKQKDTNEEHIDWLETQQELIGKIGLPNYLQTAAQED"
     gene            complement(18098..18367)
                     /locus_tag="WGQ68_000026"
     CDS             complement(18098..18367)
                     /locus_tag="WGQ68_000026"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713920.1"
                     /translation="MVKVKHLLLRILLHIKAKKRGGGSHTQTHTHQEERGKIKTDWET
                     ANKQSRFHIGIIKQKPLSSIDSNQFRFDSQTGKEKRHTRLYGYAV"
     gene            18383..18703
                     /locus_tag="WGQ68_000027"
     CDS             18383..18703
                     /locus_tag="WGQ68_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0713921.1"
                     /translation="MMGNKLTLPAELPDEQDLRAVLAYNMRLFRVNKGWSQEELARQC
                     GLDRTYVSAVERKRWNIALSNIEKMAAALGVAAYQLLLPPQERLNLMTNSADTRQMPS
                     ESGI"
     gene            18751..21309
                     /gene="glnD"
                     /locus_tag="WGQ68_000028"
     CDS             18751..21309
                     /gene="glnD"
                     /locus_tag="WGQ68_000028"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HEZ0713922.1"
                     /translation="MPANLSSALETFKRQRDAAEAHYLKANRVSVFFREYTAAVETLL
                     AALWVEHFQNSALCLMAVGGFGRGELYPCSDVDLAVVSPAPLSDGIQEQIARFVQTLW
                     DCKLMPSVKSGSVGELCESVRDDITGDTAFLEARFLFGNRQTADKLAEKMNAQRNVAA
                     FVEAKLVEMEHRHAKSQGSGAVLEPNIKSCPGGLRDIHTLLWIAKAQGLATDLPDLLK
                     QRILTRAEAGMLSHGYRRLAHIRIHLHLNAKRAEDRLLFDLQPQVAESMGYQGLNLRR
                     QSEELMRVFYRAIKTVKQLGGILTPMLRSRVSSAPMHVTLRIDDDYIQVNNQIAARHT
                     DIFLRQPEHIFKIVEIMQQRNDITALEPQTLRAWWGATRKINRSFYQNPENRRRFAGF
                     FRNGNGLTQTLRFLNLYGVLGRYLPAWEKIIGLLQHDLFHIYPVDDHILTVVRNVRRL
                     ALDMHSHELPYASALMQSFEKQDILYLAAFFHDIAKGRGGDHAIQGIADARQFAADHF
                     LTGEESDLLAWLVENHLLMSAVAQKEDIQDPDVLDAFCKRVQTHERLSALYLLTISDI
                     RGTNPKLWNAWRASLLESLFHAAGRYLTGNGGNPHTLFGRRRQEAADLLTRAAVPEKQ
                     QKKLWNALGSAYFARHQSREILWHAANLVHDFETPIVRSRILPQSDSFQVMVFMPNGP
                     RLFARLCRIFSRHGFDILAARAFITEHDYILDTFIVQIPSQHAPEDYPDIQSALEAEL
                     NSFIHGHTVAETSSYSRRISRRSRYMPIAPSITITPEEDYPDWYSVEITAVNRPFLLA
                     DMAEVFFAHNVSLRYAKISTLDERAEDSFTVFSLDLKNPKIQSSLKQALLEQLT"
     gene            21406..21867
                     /locus_tag="WGQ68_000029"
                     /pseudo
     CDS             21406..21867
                     /locus_tag="WGQ68_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            21913..21993
                     /locus_tag="WGQ68_000030"
                     /pseudo
     CDS             21913..21993
                     /locus_tag="WGQ68_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798803.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            22194..23657
                     /gene="guaB"
                     /locus_tag="WGQ68_000031"
     CDS             22194..23657
                     /gene="guaB"
                     /locus_tag="WGQ68_000031"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003767939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEZ0713923.1"
                     /translation="MRIVEKAYTFDDVLLVPAHSTVLPRDVKLQTKLTREITLNLPLL
                     SAAMDTVTEARLAISMAQEGGIGIIHKNMPPEMQARAVSKVKRHESGVVKDPVTVAPT
                     TLIREVLEMRAQRKRKMSGLPVVENGKVVGIVTNRDLRFENRVDLPVSAIMTPRERLV
                     TVPEGTSIDEARELMHTHKVERVLVLNEKDELKGLITVKDILKTTEFPNANKDSEGRL
                     RVGAAVGTGGDTEERVKALVEAGVDVIVVDTAHGHSQGVIDRVKWVKETYPHIQVIGG
                     NIATAKAALDLVAAGADAVKVGIGPGSICTTRIVAGVGVPQLTAIHNVAEALKGTGVP
                     LIADGGIRFSGDIAKALAAGAYSVMLGGMFAGTEEAPGEIELYQGRSYKSYRGMGSLG
                     AMSQGSADRYFQDKTDSTDKYVPEGIEGRVPYKGPIVNIIHQLTGGLRSSMGYLGCAN
                     IAEMHEKAEFVEITSAGMSESHVHDVQITKEAPNYHR"
     gene            complement(23732..24001)
                     /locus_tag="WGQ68_000032"
     CDS             complement(23732..24001)
                     /locus_tag="WGQ68_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713924.1"
                     /translation="MLTGRLGEGRAGHTFPKPPPDFLIWRPAHHFRPIETPVLKDCVY
                     FHRRFKGRRYSADWAQTAFRRHFHSFGVSVRIALPVRAISPCGLP"
     gene            complement(24001..26385)
                     /gene="rnr"
                     /locus_tag="WGQ68_000033"
     CDS             complement(24001..26385)
                     /gene="rnr"
                     /locus_tag="WGQ68_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEZ0713925.1"
                     /translation="MNKNIKSLNLREKDPFLSREKQRYEHPLPSREWIIELLERKGVP
                     SKIESLARELSITEDEYVFFERRLKAMARDGQVLINRRGAVCAADKLDLVKCRVEAHK
                     DGFGFAVPLTPAKDGDFVLYERQMRGIMHGDIVTVRPAGMDRRGRREGTVLDIVERAQ
                     SKVVGRFYMDRGVAILEPEDKRLNQSIVLEPDGVARFKPESGQVIVGEIEVYPEQNRP
                     AVAKIIEVLGDYADSGMEIEIAVRKHHLPHQFSEACAKAVKKIPDHVRKSDLKGRVDL
                     RDLPLVTIDGETARDFDDAVFAEKIGRNYRLVVAIADVSHYVRPDDAIDTDAQERSTS
                     VYFPRRVIPMLPENLSNGICSLNPNVERLCMVCDMVITYAGNIKEYRFYPAVMRSHAR
                     LTYNQVWKWLSGGIGHPFKAQIDTLYKLFKILQKKRFERGAVEFDSIETQMLFDDNGK
                     IEKIVPVVRNDAHKLIEECMLAANVCAADFLLKNKHTALFRNHLGPTPEKLAALREQF
                     GLLGLQLGGGDNPSPKDYAALAGQFKGRPDAELLQVMMLRSMQQAVYEPHCDGHFGLA
                     YEAYAHFTSPIRRYPDLTVHRAIKAVLNQQTYTPKKSWQALGVHTSFCERRADDASRD
                     VENWLKTYYMRDKVGEIFEGKISGVANFGIFVTLDDIHIDGLVHISDLGEDYFNFRPE
                     IMAIEGERSGIRFNMGDRVAIKVARADLDDGKIDFVLIAEGSGRGRKVKSSASAKPAG
                     TAGKGKPKTAAGKKTARGGKVRGRGASAAAESGKKAKKPIPIEVQKVKKRKGKS"
     gene            26474..26560
                     /locus_tag="WGQ68_000034"
     tRNA            26474..26560
                     /locus_tag="WGQ68_000034"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26508..26510,aa:Leu,seq:cag)
     gene            26596..26682
                     /locus_tag="WGQ68_000035"
     tRNA            26596..26682
                     /locus_tag="WGQ68_000035"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26630..26632,aa:Leu,seq:cag)
     gene            26721..26811
                     /locus_tag="WGQ68_000036"
     tRNA            26721..26811
                     /locus_tag="WGQ68_000036"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26755..26757,aa:Ser,seq:gga)
     gene            complement(27376..29187)
                     /gene="typA"
                     /locus_tag="WGQ68_000037"
     CDS             complement(27376..29187)
                     /gene="typA"
                     /locus_tag="WGQ68_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEZ0713926.1"
                     /translation="MKQIRNIAIIAHVDHGKTTLVDQLLRQSGTFRANQQVDERVMDS
                     NDLEKERGITILAKNTAIDYEGYHINIVDTPGHADFGGEVERVLGMVDCVVLLVDAQE
                     GPMPQTRFVTKKALALGLKPIVVINKIDKPSARPSWVIDQTFELFDNLGATDEQLDFP
                     IVYASGLSGFAKLEETDESNDMRPLFDTILKYTPAPSGSADETLQLQISQLDYDNYTG
                     RLGIGRILNGRIKPGQTVAVMNHEQQIAQGRINQLLGFKGLERVPLEEAEAGDIVIIS
                     GIEDIGIGVTITDKDNPKGLPMLSVDEPTLTMDFMVNTSPLAGTEGKFVTSRQIRDRL
                     QKELLTNVALRVEDTADADVFRVSGRGELHLTILLENMRREGYELAVGKPRVVYRDID
                     GQKCEPYENLTVDVPDDNQGAVMEELGRRRGELTNMESDGNGRTRLEYHIPARGLIGF
                     QGEFMTLTRGVGLMSHVFDDYAPVKPDMPGRHNGVLVSQEQGEAVAYALWNLEDRGRM
                     FVSPNDKIYEGMIIGIHSRDNDLVVNPLKGKKLTNIRASGTDEAVRLTTPIKLTLEGA
                     VEFIDDDELVEITPQSIRLRKRYLSELERRRHFKKLD"
     gene            complement(29457..30347)
                     /locus_tag="WGQ68_000038"
     CDS             complement(29457..30347)
                     /locus_tag="WGQ68_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEZ0713927.1"
                     /translation="MFPVFHLSGESRRRMLQTALRFPYVFKARAEDSHKGTFGTLAVV
                     GGSAGMSGAPVLAASAAMYLGCGKVWAGFNQDALPFAVIAGFPEIMLDTADGLSKRQD
                     INAWVVGCGLGTGRAAVGTLAGILTEHTDKPVLLDADALNILSTDAETRNLARGCKNL
                     ILTPHPAEAARLLGTTVAQVQADRTAAVRKIGAIFGATVVLKGHKTLVAASDTEIYVN
                     ESGNAGLATAGSGDVLGGIIGSLLAQGVPVFEAACAGAWLHGAAADVIKESAGIAAGL
                     LAGEIAPAARWLRNRITESM"
     gene            30411..31067
                     /locus_tag="WGQ68_000039"
     CDS             30411..31067
                     /locus_tag="WGQ68_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEZ0713928.1"
                     /translation="MKNKTKVWDLPTRLFHWLLAASLPFMWYSAKAGGDMLQWHTRVG
                     LFILFLLVFRLCWSIWGSDTARFSCFVQGWAGIRGYLKNGIPEHIQPGHNPLGALMVV
                     ALLAAVSFQVGTGLFAADENTFSTNGYLNHLVSEHTGSLMRKIHLNFFKLLAVFSAIH
                     IAAVAAYRLFKKKNLVLPMITGFKYIEGKTSIRFAGKAALAAALSVAALAAAAILLLS
                     "
     gene            31517..32959
                     /gene="cobA"
                     /locus_tag="WGQ68_000040"
     CDS             31517..32959
                     /gene="cobA"
                     /locus_tag="WGQ68_000040"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0713929.1"
                     /translation="MNYFPIFANLAGRPVLVVGGGSVAARKISLLLDAGAQVRVVANQ
                     LNAELSALAAENKILWLAEEFRAEHIRTVFLIIAASSDQALNRRVFQLAESCQKPVNV
                     VDDRDYCSFIFPSIINRNPIQIAVSSSGSAPVLARLLREKLEALLPHSLGDMAEISGR
                     WRDAVKAKLKSVTERRRFWEKQFNGRFAALVKNQQTAQAEQELAKQLEQNYQGGFVSL
                     VGAGPGDAGLLTLKGLQEIQQADVVLYDALVSDGILSLVRRDAERIFVGKRARGDRTP
                     QEDTNALMVRLAREGRRVVRLKGGDPFVFGRGGEELETLARHQIPFSVVPGITAAVGA
                     TAYAGIPLTHRDYAQSAVFVTGHRKADAPDIEWQTLARSRQTLVIYMGALKAALIAER
                     LQQHGRSPDTPAAVISQGTLPAQKTATGTLANLAELAETAPNPALIVIGEVVGLHEKL
                     AWFGENGEGENRVGQAYPALGGLNAGQRAA"
     gene            32970..33710
                     /locus_tag="WGQ68_000041"
     CDS             32970..33710
                     /locus_tag="WGQ68_000041"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HEZ0713930.1"
                     /translation="METTLFKPALWQIPHIGSGGETALAEKTETLKQRLHRIVGSHRD
                     ARFASSLAAEDMVITDLIAGENLNIGIFTLDTGLLHTETLNLLDRLGRAYPHLRIKRF
                     RPVREDADRYVESKGRFAFYDSVEARRECCRIRKIEPLNRAIAGADAWLTGQRREQSA
                     TRTELPFAEYDAGRGIGKYNPIFDWSEHDVWAYILANNVPYNDLYRQGFPSIGCDPCT
                     RPVKAGEDIRAGRWWWEGRNSKECGLHK"
     gene            33748..34671
                     /gene="cysD"
                     /locus_tag="WGQ68_000042"
     CDS             33748..34671
                     /gene="cysD"
                     /locus_tag="WGQ68_000042"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEZ0713931.1"
                     /translation="MTKTEPNNAQLDWLESESIHIIREVAAECENPALLFSGGKDSVV
                     LLALACKAFRLGSRPVKLPFPLVHIDTGHNYPEVIAFRDAQAAKLNARLIVGRVEDSI
                     AKGTVVLRKETDSRNAAQAVTLLETIEANGFDALMGGARRDEEKARAKERIFSFRDEF
                     GQWDPKAQRPELWSLYNTRLHKGENMRVFPISNWTELDIWQYIARENLELPPIYYSHR
                     REVVRRRGLLVPVTPLTPKMPSETSEILDVRFRTVGDISCTCPVESTASTPTEIIRET
                     AVADISERSATRLDDQASEAAMEKRKKEGYF"
     gene            34710..35996
                     /locus_tag="WGQ68_000043"
     CDS             34710..35996
                     /locus_tag="WGQ68_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit 1"
                     /protein_id="HEZ0713932.1"
                     /translation="MTAQHQTPLRFITAGSVDDGKSTLIGRLLYDSKALLSDQIKTLE
                     SGKSKGLKETIDFSILTDGLEAEREQGITIDVAYRYFSTAKRKFIIADTPGHEQYTRN
                     MVTGASTASAAVVLVDASQLDFGAQPLQLLPQTKRHSAILRQLNCPHIVVAVNKMDLL
                     DYSEDKFNAIVEAYRRLAEQLGLKDVHFVPVSALLGDNLVYPGGNMPWYRGEPLLVIL
                     ENLPGADETGRSAADFYFPVQLVVRQDADKADDFRGYQGRIERGSVAVGQTVRIEPNG
                     LTAEVSEIITPKGEVAQAFAGEAATLRLDRDIDVSRGDLFVDKNSPLAPQKHLEATLC
                     WFDERPLNTARKYLLKHGTQTVPAKVGEIESVLDVRTLEQEAGAESLKMNDIAKVRIN
                     LQKPVTATPYAENTAAGSFILIDEATYGTVAAGMIL"
     gene            35993..37807
                     /locus_tag="WGQ68_000044"
     CDS             35993..37807
                     /locus_tag="WGQ68_000044"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HEZ0713933.1"
                     /translation="MSEHDMQNTNPPLPPLPPEITQLLSGLDAAQWAWLSGYAWAKAG
                     NGASAGLPALQTALPAAEPFSVTVLSASQTGNAKSVADKASDSLEAAGIQVRRAELKD
                     YKAKNIADERRLLLVTSTQGEGEPPEEAVVLHKLLNGKKAPKLDKLQFAVLGLGDSSY
                     PNFCRAGKDFDRRFEELGAKRLLERVDADLDFAAAADGWTGRIVARLKEEAAKNRATP
                     APQTTPPAGLQTAPDGRYCKADPFPAALLANQKITARQSDKDVRHIEIDLSGSDLHYL
                     PGDALGVWFDNDPALVREILDLLGIDPATEIQAGGKTLPVASALLSHFELTQNTPAFV
                     KGYAPFADDDELDRIAADNAVLQGFVQSTPIADVLHRFPAKLTAEQFAGLLRPLAPRL
                     YSISSSQAEVGDEVHLTVGAVRFEHEGRARVGGASGFLADRLEEDGTVRVFVERNDGF
                     RLPEDSRKPIVMIGSGTGVAPFRAFVQQRAAENAEGKNWLIFGNPHFAADFLYQTEWQ
                     QFAKDGFLHRYDFAWSRDQEEKIYVQDKIREQAEGLWQWLQEGAHIYVCGDAAKMAKD
                     VEAALLDVIIGAGHLDEEGAEEYLDMLREEKRYQRDVY"
     gene            37834..39603
                     /gene="cysI"
                     /locus_tag="WGQ68_000045"
     CDS             37834..39603
                     /gene="cysI"
                     /locus_tag="WGQ68_000045"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HEZ0713934.1"
                     /translation="MTVQTKTKGLAWQEKPLSDNERLKTESNFLRGTILDDLKDPLTG
                     GFKGDNFQLIRFHGMYEQDDRDIRAERAEAKLEPLKFMLLRCRLPGGIIKPSQWIELD
                     KFARENSHYRSIRLTNRQTFQFHGVPKAKLQTMHRLLHKLGLDSIATAADMNRNVLCT
                     SNPIESELHRQAYEYAKKISEHLLPRTRGYLDVWVDGKKVQSSDDFLQEDEPILGKTY
                     LPRKFKTAVVIPPLNDVDCYGNDLDFVAISDGNGQLAGFNVLAGGGLSMEHGNTKTYP
                     NISLELGFVPPEHALKAAEAVVTTQRDFGNRSDRKNARTRYTIQNMGLDNFRAEVERR
                     MGMPFEPIRPFKFTGRGDRIGWVKGIDGNWHLTLFIESGRLVDEGGKQLLTGVLEIAK
                     IHKGDFRITANQNLIVANVAEADKAKIEELARTYGLIRNDVSKLRENAMSCVSFPTCP
                     LAMAEAERVLPDFIDELDKIMAKHGTSDDYIVTRITGCPNGCGRAMLAEIGLVGKAVE
                     RYNLHIGGDREGVRIPRLYKENITLPEILAELDDLIGKWAAERNIGEGFGDFAIRTGI
                     VKPVLNAPVDFWDVSKAVAIARA"
     gene            39776..41635
                     /gene="ilvD"
                     /locus_tag="WGQ68_000046"
     CDS             39776..41635
                     /gene="ilvD"
                     /locus_tag="WGQ68_000046"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEZ0713935.1"
                     /translation="MPEYRSKTSTHGRNMAGARALWRATGVMETDFGKPIIAVANSFT
                     QFVPGHVHLHNMGQLVAREIEKAGAIAKEFNTIAIDDGIAMGHSGMLYSLPSRDLIAD
                     SIEYMVNAHCADALVCISNCDKITPGMLIAAMRLNIPTIFVSGGPMEAGKVIGVANIQ
                     PERRLDLIDAMIESADDNVSNRQVEEVEQNACPTCGSCSGMFTANSMNCLTEALGLSL
                     PGNGSYLATHAGRKELFLEAGRMIVEITKRYYEQDDETVLPRSIATKKAFENAMTMDI
                     AMGGSTNTILHLLAVANEAGVDFKMADIDRLSRVVPCICKTAPNNHDYYMEDVHRAGG
                     IFAILKELDKAGKLHTDVHTIHAPTLKDAIEKWDVTNPENTRAIERFKAAPGGVRTTQ
                     AFSQNRMWKTLDLDREKGCIRDVAHAYSQDGGLAVLFGNIAERGCVVKTAGVDESILK
                     FTGRARVFESQEDAVEGILGNQIVAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLG
                     KACALLTDGRFSGGTSGLSIGHASPEAAEGGAIGLVHEGDTVEIDIPNRSIHLAISDE
                     ELAARRAEMEARGSKAWKPKNRDRYVSAALRAYGAMATSADKGAVRDVAQIER"
     gene            complement(41698..41958)
                     /locus_tag="WGQ68_000047"
     CDS             complement(41698..41958)
                     /locus_tag="WGQ68_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0713936.1"
                     /translation="MTKAVRRKNVQTPKICYAFRTPSAYGQYLADAGFDYLSFANNHS
                     NDFGAQGITATAASGSSFTYSIALKPLPITRPKLRKIPPSPR"
     gene            42281..42916
                     /locus_tag="WGQ68_000048"
     CDS             42281..42916
                     /locus_tag="WGQ68_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEZ0713937.1"
                     /translation="MNIKYSGIENRFETAILKKNGVRSGFVSFAPNLAAVKLNDYAKV
                     RKRITKTKQKADIVIVMFHGGAEGKQAEHLPFDTEIFYGENRGNVVEFARLAVDSGAD
                     VVFGQGPHVTRAVELYRDRFISYSGGNFATYGAINTSGISGIAPIFKIITDKQGKFIS
                     GTIIPITQADDKIPKIDPEKTVIGRIIYLNRSDFPNGNGLDVSPDGNITRR"
     gene            complement(42995..44047)
                     /gene="bioB"
                     /locus_tag="WGQ68_000049"
     CDS             complement(42995..44047)
                     /gene="bioB"
                     /locus_tag="WGQ68_000049"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HEZ0713938.1"
                     /translation="MTVSPVALRRKTECKPHPTARYWKKCDVEALFGLPFLELVHQAA
                     EVHRQNFNPREIQLSTLLSIKTGGCPEDCAYCPQSAHHNTNLGKEQMMDVDEIVEKAK
                     IAKSRGASRFCMGAAWRGPKPKDVETVSAIIKAVKGLGMETCGTFGMLEEGMAEDLKE
                     AGLDYYNHNLDTDPDRYNDIIHTRRHEDRMDTLGKVRNAGLKVCCGGIVGMNETRAER
                     AGLIASLANLDPQPESVPINRLVKVEGTPLDDAEDLDWTEFVRTIAVARITMPQSYVR
                     LSAGRSNMPEAMQAMCFMAGANSIFYGDKLLTTGNPDEDGDRILMEKLNLYPLQFELE
                     GEVAEVEKASGIKVDY"
     gene            complement(44254..45630)
                     /gene="mpl"
                     /locus_tag="WGQ68_000050"
     CDS             complement(44254..45630)
                     /gene="mpl"
                     /locus_tag="WGQ68_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HEZ0713939.1"
                     /translation="MKHIHIIGIGGTFMGGIAAIAKEAGFEVSGCDAKMYPPMSTQLE
                     ALGIGVHEGFDAAQLEEFKADVYVIGNVAKRGMDVVEAILNRGLPYISGPQWLAENVL
                     HHHWVLGVAGTHGKTTTASMLAWVLEYAGLAPGFLIGGVPENFSVSARLPQTPRQDPN
                     SQSPFFVIEADEYDTAFFDKRSKFVHYRPRTAVLNNLEFDHADIFADLGAIQTQFHHL
                     VRTVPSEGLIVCNGRQQSLQDTLDKGCWTPVEKFGTEHGWQAGEANADGSFDVLLDGK
                     KAGHVAWSLMGGHNRMNALAVIAAARHAGVDIQTACEALSTFKNVKRRMEIKGTVNGI
                     TVYDDFAHHPTAIETTIEGLRQRVGNARILAVLEPRSNTMKLGTMKSALPESLKEADQ
                     VFCYAGGVDWNVAEALAPLGGRLHVGKDFDAFVAEIVKNARTGDHILVMSNGGFGGIH
                     TKLLDALR"
     gene            complement(45692..45997)
                     /locus_tag="WGQ68_000051"
     CDS             complement(45692..45997)
                     /locus_tag="WGQ68_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713940.1"
                     /translation="MRVSKIIGSMLLVTAVQTVFSANVYACRHNGKTSYSQTPGKHCT
                     NAGLGRDRVYSSVRPAVKDRAEDAGVGDYSDTVRDEHVQNPRENTHKDASNTGAKTH"
     gene            complement(46009..46362)
                     /locus_tag="WGQ68_000052"
     CDS             complement(46009..46362)
                     /locus_tag="WGQ68_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713941.1"
                     /translation="MKNCEFFYDPTRAIYDSGADYLTREKHRLVVIANSAWGLLLNLS
                     CYYDEVLEKRKIPFGKQEIDDDMDKVSAHKRKFKDISEIKVGDGWEYPFNYEQGMKEL
                     DEVLLKYIPFFEEER"
     gene            complement(46624..46821)
                     /locus_tag="WGQ68_000053"
     CDS             complement(46624..46821)
                     /locus_tag="WGQ68_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713942.1"
                     /translation="MPSEKIYYGVLIFLCIASMLLSPFFYAGALKPKKAALRKDGQWK
                     LILLSNTVAAAVLFWMWWKWF"
     gene            complement(46831..47616)
                     /locus_tag="WGQ68_000054"
     CDS             complement(46831..47616)
                     /locus_tag="WGQ68_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0713943.1"
                     /translation="MKHISSANNEHIRHLHRLLSQGKFRRQYAQTVLEGVHLLQVFLQ
                     SGGRPVGVYIPEAKMPSEEVRKLTAVLPEDGVFSVSDGILKKISSLTCADDVLALIDI
                     PDAGALPAGGDCVVLDGVQDPGNVGTVLRSAAAAGIGAVVLGKGCADAWSPKVLRAGM
                     GAHFLSEIYPQADLEIWLASYKGRVFATALREEKQAVLYNEDLREPTAWVFGNEGAGV
                     GKAVLDRADKCVRIPMHDATESLNVAMAATICLFEQMRQRSAY"
     gene            complement(47667..48977)
                     /gene="tilS"
                     /locus_tag="WGQ68_000055"
     CDS             complement(47667..48977)
                     /gene="tilS"
                     /locus_tag="WGQ68_000055"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEZ0713944.1"
                     /translation="MLTLDAFEQCLKDCFPQGLNGKKTAVALSGGLDSVVLLHLLVRA
                     GKKGGFIPDALHIHHGLSPRADDWADFCQNYCDMLGVGLETVKVCVEKNGLGIEAAAR
                     QKRYAAFAEKGFDVLALAHHRDDQIETFMLAVSRGGGLRALAAMPAVRPFGEKGIIWR
                     PLLPFSRQDIWDYAQKHGLPNIEDESNSDTAYLRNRFRHRILPELSAQIPHFGRHVLN
                     NVRALQEDLALLDEIIGQDCRWVCGAGYFDTARWLTFSPRRKTHILRHFLKENGIPVP
                     NQNALADIARVLTEAKTGRWNVQGFELHHYAGRLFVFRPEKTDKLRFLKDRQISGNLR
                     EILAGQGFVLKRHPFGLPGHLLEQDGILRTVAASDRLAVGGIHKDVKKILQEKRVLPA
                     LRPFWPVVADKDNRPLALANYGTDFQISVSDGILPVHPDFPVLF"
     gene            complement(49045..50004)
                     /locus_tag="WGQ68_000056"
     CDS             complement(49045..50004)
                     /locus_tag="WGQ68_000056"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEZ0713945.1"
                     /translation="MKPVFLDFEQPIAELTNKIDELRFVQDESAVDISDEIHRLQKKS
                     NDLTKSIFSKLTPAQVSQVSRHPQRPYTLDYIDALFTDFEEMHGDRHFADDHAIVGGL
                     ARFNGQSVMVVGHQKGRDTKEKIRRNFGMPRPEGYRKALRLMKTAEKFGLPVMTFIDT
                     PGAYPGIGAEERGQSEAIGKNLYELTRLHVPILCTVIGEGGSGGALAVAVGDYVNMLQ
                     YSTYSVISPEGCASILWKTAEKAADAAQALGITADRLQKLDLVDTVIKEPLGGAHRDF
                     GQTMKNVKAVLEKQLHEAQSIPLADLLSRRFDRIMAYGKFSEQ"
     gene            50215..50616
                     /locus_tag="WGQ68_000057"
     CDS             50215..50616
                     /locus_tag="WGQ68_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0713946.1"
                     /translation="MKDKHDSSAMRLDKWLWAARFFKTRSLAQKHIELGRVQVNGSKV
                     KNSKTIDIGDIIDLTLNSLPYKIKVKGLNHQRRPASEARLLYEEDAKTAALREERKQL
                     DQFSRITSAHPDGRPTKRDRRQLDRLKKGDW"
     gene            complement(50684..50881)
                     /gene="iscX"
                     /locus_tag="WGQ68_000058"
     CDS             complement(50684..50881)
                     /gene="iscX"
                     /locus_tag="WGQ68_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HEZ0713947.1"
                     /translation="MKWTDTRRIAEELYDLHGETIDPRTVRFTQLRDLIMALPEFDDD
                     PARCGERILEAVQQAWIDEAE"
     gene            51094..51681
                     /locus_tag="WGQ68_000059"
     CDS             51094..51681
                     /locus_tag="WGQ68_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEZ0713948.1"
                     /translation="MNNMFAAKLSKLVYTASDHPDSLSEMEEFDRLILLIRKLYQILD
                     GQHILSRVTVCLHHQNRRGLIALDKAAAGCDAAMLRAQRWRIYTQIAERLAGSGGGFT
                     VTAESVSAACPELEGRYLELVRRTATSFGFVQVQEYPAGGQEETQAVPDAASDHSVRK
                     PIKINIEAKVERYPAYRPTAALKVTRRRFFRLQNR"
     gene            complement(51968..52309)
                     /gene="fdx"
                     /locus_tag="WGQ68_000060"
     CDS             complement(51968..52309)
                     /gene="fdx"
                     /locus_tag="WGQ68_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HEZ0713949.1"
                     /translation="MPKITVLPHTTLCPEGAVIEDAPEGKTVLDVLLDHDIEVDHACE
                     KSCACTTCHVIIRKGFDSLEEPTELEEDLLDQAWGLEADSRLSCQAVVAGEDLIVEIP
                     KYTINHAREEH"
     gene            complement(52364..53125)
                     /locus_tag="WGQ68_000061"
     CDS             complement(52364..53125)
                     /locus_tag="WGQ68_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2314 domain-containing protein"
                     /protein_id="HEZ0713950.1"
                     /translation="MMGDSVIYYVEQADKPVNRAGERARKTFKYFWRELFWERRRIIP
                     ALDFAMVKAPFFQDGEDGEICEHMWIDDIYFDGLYIYGVLNNEPGELTNVEQGESVCV
                     PVDDISDWMFVCNGIPYGGFTIQAMRGQMTEEERTEHDAAWGIDFGDPGQVLLVYEEK
                     EHPENLEEHPMCRNCIDDFRQQLSQNPDFLHEQDEDGYTPLHHEAIAGNALMVQAMLE
                     YGANPASKTSEGYTALDFARLAGWQNVADLLEPRH"
     gene            complement(53162..53785)
                     /locus_tag="WGQ68_000062"
     CDS             complement(53162..53785)
                     /locus_tag="WGQ68_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0713951.1"
                     /translation="MFVNSLYKIGISEVSNFEGRMRHLENNGYANVAGLERILAVKTD
                     NYKEKENLLHEIFSKSRIGDTELFAVDENLVKRLFLSLRGEIVFPKNETAESEFEKSV
                     HERRQEGNAGSGRKQLLDLVRRGHREYPYALPRLLAGAASYKPKKSKIRLFKEAYFGK
                     SGTRLTDEIADGIHIYTCFSRADLEKAYSEYLELFKSESDAEGRKPQ"
     gene            complement(54048..55910)
                     /gene="hscA"
                     /locus_tag="WGQ68_000063"
     CDS             complement(54048..55910)
                     /gene="hscA"
                     /locus_tag="WGQ68_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HEZ0713952.1"
                     /translation="MALLQISEPGMSAAPHRHRLAAGIDLGTTNSLVATVRSGSAACL
                     PDAEGRITLPSVVRYLENGGIEVGKTALSAQKTDPLNTVSSAKRLIGRTLADLHQNTH
                     YLPYRFGDNQRVIELHTRQGVKTPVEVSAEILKTLKSRAEETLGGDLVGVVITVPAYF
                     DDAQRQATKDAARLAGLNVLRLLNEPTAAAIAYGLDNASEGTFVVYDLGGGTFDVSVL
                     QLTKGLFEVKATGGNSALGGDDFDHRLFCRLLEQNGLSQLNEQDSQLLLSLVRAAKEQ
                     LTTQTEARIQATLSDGMAIDTSISRAEFHNLTQHLVMKTLEPVTQALKDAGVGKNEVK
                     GVIMVGGSTRMLHVQQAVATFFGQTPLDNLNPDEVVALGAAIQANVLAGNKTDGEWLL
                     LDVTPLSLGLETYGGLAEKIIPRNSTIPTARAQDFTTFKDGQTAMTIHVVQGERELVA
                     DCRSLAKFTLRGIPPMAAGAARIRVTFQIDADGLLSVSAQEQSTGVHAQIEVKPSYGL
                     DDDTITQMLKDSMSNAAEDMAARARAEAVVEAESLTDAVNAALELDSDLLDAEELQQI
                     RQGIADLQGRLKDGKAEDIRAAAAKLGSITDNFAAKRMNRNIQRALTGQSVDNI"
     gene            56081..56953
                     /gene="hpnD"
                     /locus_tag="WGQ68_000064"
     CDS             56081..56953
                     /gene="hpnD"
                     /locus_tag="WGQ68_000064"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HEZ0713953.1"
                     /translation="MKGLDYCRQKAEESRSSFLSGFRFLTQEKRDAVTILYAFCRELD
                     DVVDECSNPDVAQATLNWWRGDLDKVFGGAMPEHPVNQALRQVKETFKLPKYELEALI
                     DGMQMDLVQARYGSFEELKLYCHRVAGVVGYLIARILGFSDGKTLQYADKMGLALQLT
                     NIIRDVGEDARRGRIYLPMEEMQRFDVPASVILQCSPTGNFAELMAFQIKRARETYRE
                     AVSLLPAADKKAQKVGLVMAAVYYELLNEIDRDGAQNVLKYKIALPSPRKKRIALKTW
                     LFGFKPRPGTPERA"
     gene            57032..58345
                     /locus_tag="WGQ68_000065"
     CDS             57032..58345
                     /locus_tag="WGQ68_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEZ0713954.1"
                     /translation="MMNTPHPRPKIAVIGAGWAGLSAAVTLARHADVTLFEAGRQAGG
                     RARALAGNTDGFGFLDNGQHILLGAYRGVLRLMKTIGSDPRAAFLRVPLHWHMHGGLQ
                     FRTLPLPAPLHILGGVLLARRVPSAFKAKLLADMSDLQKSARLGQPDTTVAQWLKQRN
                     VPRAAVMQFWQPLVWGALNTPLETASLRVLCNVLSDGVLTKKSGSDYLLPKQDLGAIV
                     AEPALAELQRLGADIRLETRVCRLNTLPDGKVLVNGEAFDAAVPATAPYHAAALLPEG
                     TPEHVQTAYQNLRYHAITTVYLRYAEPVRLPAPLTGLADGTVQWLLCRGRLGLPENEV
                     SAVISVSDRVGAFANRAWADKAHADLKRILPHLGEPEAVRVITEKRATTAADAPPPDL
                     SWLHRHRIFPAGDYLHPDYPATLEAAVQSGFASAEACLQSLIDAV"
     gene            58429..59148
                     /locus_tag="WGQ68_000066"
     CDS             58429..59148
                     /locus_tag="WGQ68_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0713955.1"
                     /translation="MATLSNKTILVTGASQGLGEQVAKAYAAAGATVILVARHQKKLE
                     KVYDAIVEAGHPEPFAIRFDLMSAEEKEFNQFAATIAEATQGKLDGIVHCAGYFYALS
                     PLDFQTVAEWVNQYRINTVAPMGLTRALFPLLKQSPDASVIFVGESHGETPKAYWGGF
                     GASKAALNYLCKVAADEWERFGNLRANVLVPGPINSPQRIKSHPGEAKSERKSYGDVL
                     PAFVWWASTESKGRSGEIVYL"
     gene            59313..59984
                     /locus_tag="WGQ68_000067"
     CDS             59313..59984
                     /locus_tag="WGQ68_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEZ0713956.1"
                     /translation="MKTVSTAVVLAAAAVSLTGCATESSRSLEVAKVASYNTQYHGVR
                     TPISVGTFDNRSSFQKGIFSDGEDRLGSQAKTILVTHLQQTNRFNVLNRTNLNALKQE
                     SGISGKAHNLKGADYVVTGDVTEFGRRDVGDHQLFGILGRGKSQIAYAKVALNIVNVN
                     TSEIVYSAQGAGEYALSNREIIGFGGTSGYDATLNGKVLDLAIREAVNSLVQAVDNGA
                     WQPNR"
     gene            59994..60362
                     /locus_tag="WGQ68_000068"
     CDS             59994..60362
                     /locus_tag="WGQ68_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4810 domain-containing protein"
                     /protein_id="HEZ0713957.1"
                     /translation="MNPKTLSRLSLCAAVLALTACGGNGQKSLYYYGGYPDTVYEGLK
                     NDDTSLGKQTEKMEKYFVEAGNKKMNAAPGAHAHLGLLLSRSGDKEGAFRQFEEEKRL
                     FPESGVFMDFLMKTGKGGKR"
     gene            60359..61006
                     /locus_tag="WGQ68_000069"
     CDS             60359..61006
                     /locus_tag="WGQ68_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF799 domain-containing protein"
                     /protein_id="HEZ0713958.1"
                     /translation="MKPLILGLAAVLALSACQVQKAPDFDYTSFKESKPASILVVPPL
                     NESPDVNGTWGVLASTAAPLSEAGYYVFPAAVVEETFKENGLTNAADIHAVRPEKLHQ
                     IFGNDAVLYITVTEYGTSYQILDSVTTVSAKARLVDSRNGKELWSGSASIREGSNNSN
                     SGLLGALVSAVVNQIANSLTDRGYQVSKTAAYNLLSPYSHNGILKGPRFVEEQPK"
BASE COUNT        15580 a        16424 c        14868 g        14429 t
ORIGIN      
        1 gtggtaaagc ggccatcgtg ttcggcaaac aactcgtttt tcatacggcc taaaacttgc
       61 caataggctt tcaatgcgtc catatcgtgc gcaggtatgc cgccatccat aaagataggc
      121 ggcgagattt tacaggtctt cgacttctcc gctgtcgata tagcgaggca gattaaggtt
      181 gtaatcttgt gctgcgattt cgcttaaatg caccatacgg ctgtaacggg gaatttcttc
      241 ctgttttgtg aaggtgtcga tgattttgtg aatgtcttgt tcgcgcagac ggtttttgtt
      301 gccgtcttta ataaagccgc gcgatgcgtc aatcataaac acgctgccgc cactgataac
      361 ttggtttgtt ccctcttcgg caaattgggc ggtttgggcg tgttctttgt cgatgacgat
      421 gatgcaggca ggaatgcccg tgccgtaaaa caggttggca ggcagcccga taatgccttt
      481 aataaggtca aggttaagca attccgtgcg aatacgcgct tcggcattgc cgcgaaacag
      541 cacaccgtgc ggaagaataa tcgcaccttt gccgcttggt ttcaggcttt tgagcagatg
      601 cagcaaaaag gcgtaatcgc cgtttttttc gggcgggata aggcggattg gcaacggcaa
      661 aatcgaaggt cttaagcccg tcgttttcat cacggaaaga cgaatcggac aaggtgttcc
      721 cggtgttgat ttcggcggtt tcgttgttgt gcaaaatcat attcatacgg gcaagggacg
      781 cggttgccac atctttttcc tgaccgtaaa ggctgatttg gctgccggct tgggcggcgg
      841 ctttgagcaa cagcgaaccc gagccgcagg tcgggtcata aacgctggtg ctggacggca
      901 atctgcgctg attccgataa tcttcgccat aatgcgggag acttcggcag gcgtgtaaaa
      961 ctgccctttg gatttgcctg actcggtcgc aaaatggcgc atcaggtatt cgtaggcatc
     1021 acctaacaaa tcgtcgtctt cggcttggtt ggaagaaagg ttgagctttt taaaaatgcc
     1081 gacaagcctg ctcaaacggt cgatcatctc cttaccttta cccagtttgt cttcgtcgtt
     1141 gaagtcggta acgttgatca cgccttttaa gtcgttggct tcggcaattt gggcgataat
     1201 cttattcagg cggtcgccga tgtcggcggt gtttttgagt ttgacgatgt cgtcaaaagt
     1261 cgtaccggcg tgcagctcaa tcatgccgcc gccgtactta tgcttatcag aaacatattt
     1321 taaaaacaat agcgtaagca catagttctt tataaaggct cgcatccata ccgccgcgca
     1381 actcgtcgca gcccgcccaa agtgatgcat aaagctgatt tttttttgat tgccataagg
     1441 ttttgcctaa taaatggaaa ttacaaatca aacgcgattt taacacgccg gcggcaacgg
     1501 ctatatcggc attcacagct tccagtttat cggatcgata ccgtgccggc tcaaatagct
     1561 gtttgcctgt gaaaaatggc ggcaaccgaa aaaaccgcga tatgccgaca gaggagacgg
     1621 atgcggtgcg gtcagtatca aatgattttg actgtctatc agtttccttt tttgttgcgc
     1681 atacccaccc cacaacatga agacaaggtg cttgcggtgc gccgcaagct gcctgatgac
     1741 ggtatcggta aagcgttccc agcctaaaag ggcgtgcgaa tgcgcctgtc ctgcacgcac
     1801 cgtcaaaacc gtgttcagaa gcaatacgcc ctgctccgcc cacgctgtca gacagccgtg
     1861 cgcgggaatg gaaaagcctt cgatgtcggt ttccaactcc ttgtagatat tgagtaaaga
     1921 cggcggtatg cggataccct gccggacgga aaatgccaaa ccgtgcgcct gccctgcccc
     1981 gtgatacgga tcttgtccga gaatgacggc tttgacccgg tcgaacgctg tcaggcggaa
     2041 tgcgttgaac acatccgccg ccggcggata gatgatttgt cccgacaaac gttcctgcct
     2101 gactgcattt aaaatttcct gaaaatacgg ctgctgtttt tcgccgccga gtgcatcgtg
     2161 ccaagtgtcc atatctgccc tatcctttca aattcggcta tactgctgat attgcgttat
     2221 tttgtcaaac gacgcgcttt gtggggaaat gtatgactgt atggtttgtt gccgctgttg
     2281 ccgtcttaat catcgaatta ttgacgggaa cggtttatct tttggttgtc agcgcggctt
     2341 tggcgggttc gggcattgct tacgggctga ccggcagcac gcctgccgcc gtcttgaccg
     2401 ccgctctgct ttccgcgctg ggtatttggt tcgtacacgc caaaaccgcc gtgggaaaag
     2461 ttgaaacgga ttcatatcag gatttggatg ccgggcaata tgccgaaatc ctccggcacg
     2521 taggcggcaa ccgttacgaa gttttttatc gcggtacgca ctggcaggcg caaaatacgg
     2581 ggcaagaaga gcttgaaccg ggaacgcgcg ccctcattgt ccgcaaggaa ggcaaccttc
     2641 ttattatcac acacccttaa cactcggagg aattatggaa tttttcatta tcttgttggt
     2701 agccgtcgcc gttttcggtt tcaaatcctt tgttgtcatc ccacaacagg aagtccacgt
     2761 tgtcgaaagg ctcgggcgtt tccatcgcgc cctgacggcc ggtttgaata ttttgattcc
     2821 ctttatcgac cgcgtcgcct accgccattc gctgaaagaa atccctttag acgtacccag
     2881 ccaagtctgc atcacgcgcg acaatacgca gctgactgtt gacggtatca tctatttcca
     2941 agtaaccgac cccaaactcg cctcatacgg ttcgagcaac tacattatgg cgattaccca
     3001 gcttgcccaa acgacgctgc gttccgttat cggacgtatg gagttggaca aaacgtttga
     3061 agaacgcgac gaaatcaaca gtactgttgt tgcggctttg gacgaggcgg ccggggcttg
     3121 gggtgtgaag gttttgcgtt atgagattaa agacttggtg ccgccgcaag aaatccttcg
     3181 ctcaatgcag gcgcaaatta ctgccgaacg cgaaaaacgc gcccgtatcg ccgaatccga
     3241 aggtcgtaaa atcgaacaaa tcaaccttgc cagtggtcag cgcgaagccg aaatccaaca
     3301 atccgaaggc gaggctcagg ctgcggtcaa tgcgtcaaat gccgaaaaaa tcgcccgcat
     3361 caaccgcgcc aaaggtgaag cggaatcctt gcgccttgtt gccgaagcca atgccgaagc
     3421 catccgtcaa attgccgccg cccttcaaac ccaaggcggt gcggatgcgg tcaatctgaa
     3481 gattgcggaa caatacgtcg ccgcgttcaa caatcttgcc aaagaaagca atacgctgat
     3541 tatgcccgcc aatgttgccg acatcggcag cctgatttct gccggtatga aaattatcga
     3601 cagcagcaaa accgccaaat aagccgtgat gaaatgccgt ctgaagctct gttttcgggg
     3661 ttcagacggc atttttattt aaactcgggc agggttgtat tccgcttcaa atatagtgga
     3721 ttaacaaaaa ccagtacggc gttgtcctat ttgtactgtc tgcggcttcg tcgccttgtc
     3781 ctgatttttg ttaatccact atatgtttcg gaaccgccta cctgccccgg taaaaactgc
     3841 cggcaaaata tttctgcacg ccctcgcgaa gcaagaccaa ggttacggcg gccaaaggca
     3901 atcctgccaa cattccgacg aaacccatca gctgtccgaa cgccatcagg gaaaagataa
     3961 cccaaaacgg cgacagcccg atacggtctc ccacgatttt cggcgtaatg aaaaaacttt
     4021 cgagaaactg tcctacggca aaaaccgccc aaaccgatag gatgccgttc cacgaaccga
     4081 actggagcaa ggcggcgacg gttgccagca gcagtcctgt aaacgcgccc aaatagggaa
     4141 caaaaaccaa aataccggca accataccga ttgcaaaccc cgaatccagc ccgaccagca
     4201 ccaaccccaa gccgtaaacc aaacccataa tcagcatcac cagaagctgc ccgcgcaaaa
     4261 attcgcccaa tacctcgttc aaattacccg taatgcgcgt ataagcaccg gcaaaacgcc
     4321 tcggaaccag tttggcgatg ccgcacgacc accgctgcca atccagcagg aaataataaa
     4381 gcagcaaggg cagcagcagt aggttgccga tactgctgac aatattgccg ccctgcctca
     4441 tcaaaacggg aaaccacgcc ttaagcgcgt tgctcaactc gcccgtatgc gcctgaagcc
     4501 acgcaataat agatgcctga tcgatttcca tatatccgcc gattgtattt ttcaaccacg
     4561 gcagcagcgt gttctgcata aaaccgatta attggggcag gcgcgatgcc aaattgttga
     4621 actgcccgac cagcataggg acgataatca acaataatgc caacaacaaa atcaaggaaa
     4681 acaccatcac agacatcgaa gcggatgcac ggttcaaacc ctttttctgc aaccattcga
     4741 ccaaagggtc caatacatac gccagcaccg ccgcaaccgc aaacggagtc aaagtatcgc
     4801 cgagcgcgaa aaccagccaa accaaggcgg caaacgccgc accggcaccc atccacggct
     4861 tgatgccccg ccctttcctc cgatacataa aaaccaccct tctaaaaata atattgcgcg
     4921 ccagtatagc agaaccgccg cgccgtcaaa aaagcctccg ccttcccgat agcctgttat
     4981 cgcggtttag gctaaaataa cgcacatccg atgccgtctg aaaggcaaac ccgccttcag
     5041 acggcatacc gtacacaaca aggcagcaca tcatgaccga acaaaaaaac aaaacatccc
     5101 tcatcgaatt tccctgcacc ttcccattga aagtaatggg cgcggtgcat cccgagttcg
     5161 agcaggcggt attggacacc gtccgccttc acgcccccga cacgcaggcg caccacatca
     5221 ccacgcgtcc gagcagcaaa ggcaactata ctggcgccac cgtacaggta aaggttgaaa
     5281 accaagaaca attggacaac atctaccgtg cgctgacttc gcacgaactg gtcaaagtgg
     5341 tactttagag atgaaaatca tacacaaagg tttggtcgaa tatctgccga cttttgaagc
     5401 gatgaaaacc tttaatgccg gacggaatgc cgacaccgaa gacgaactgt gggtcgtcga
     5461 acacccgccc gtgttcacac aaggactggc gggaaaaccc gaacacctgc tgattcgcga
     5521 cgacattccc gtcgtccaaa tcgaccgggg cgggcagatt acctatcacg ggcccgggca
     5581 attggtcgtt tatacgatga ttgatttcaa acggcgcaaa accagtgtta gaaacatcgt
     5641 ttccgcgctt gaaaacagca tcatcgccac attggcagaa tacggcatcg aagcggcggc
     5701 agaccccaaa cgccccggcg tttatgtcgg agaacgcaaa atcgcctcac tggggctgcg
     5761 tatcaaaaac ggctccgtct atcacgggct tgcgctcaac gtcaatatgg atttaagccc
     5821 gtttacccac atcaacccct gcggctacgc cggtatggaa atgacgcaaa tcgcggattt
     5881 tgtccaaccc tgccccacgc cggacgaagt cgccgccaaa ctcaccgcac accttgaaac
     5941 acaattcaca ccgaaagcag acaacaatga gtgaaatcaa aaccgacgac cccaaacgcg
     6001 gcatcaaact cagaggcgcg gacaaaaccg cgcgcatccc catcaaagtc gtcccccttc
     6061 aggaaaaact gaaaaagccc gaatggatac gcgccaaact cccatcgcgc aaattctttg
     6121 aaatcaaaga cattttgcgc gaacaaaaga tgcacaccgt ttgcgaggaa gcctcctgcc
     6181 ccaacatcgg cgaatgcttc agcaaaggca cggcgacctt catgattatg ggtgacatct
     6241 gcacccgccg ctgcccgttc tgcgacgtgg gacacggtcg gcccaatatg ctcgaccccg
     6301 acgaaccgag aaacctcgcc gaatccgtca aagccatgaa cctgcgttac gtcgtcatca
     6361 cctccgtcga ccgcgacgac ctgcgcgacg gcggcgcaca gcatttcgcc gactgcatca
     6421 aagccatccg cgaaaccagc ccgaacacca aaatcgaaat cctcgtcccc gacttccgag
     6481 gacgcttgga catcgcactc aaaatccttg ccgaaacccc gcccgacgtg atgaaccaca
     6541 acctagaaac ccatccgagc ctgtacagaa aagcccgtcc cggtgccaat tatcaacatt
     6601 ctttagactt attaaaacgt tataaagaaa tgatgccgca catcccgacc aaatccggca
     6661 tcatggtcgg cttgggcgaa acagacgaag acgtgcgtga aattatgcgc gatatgcggg
     6721 cgcacaatat cgaaatgatt accatcggac agtacctcca gccttcagac ggacacctgc
     6781 ccgtcctgcg ctacgttacg cccgagcagt tcaaaatctt tgaaaaagaa gcatacgaac
     6841 tgggcttcag caatgccgcc atcggcgcga tggtacgttc cagctaccac gccgacgaac
     6901 aggctgccga agccttacgc gaatgtgaat tctgacgcta tttgagactg aactacgcta
     6961 tttgcgactt tataacgcta agttcgactg ggcaaatatg gatcaacaac cagttattga
     7021 aattgccgaa ttcccacatg ataataaatt gtggcgaatt gatgggttag ggaagatttt
     7081 ttcccaattg aaaaataaca ataacctgtt tattgaagtc tatttacgcc cagttaactt
     7141 tcacgataat tctgtatata actttgctaa atttgatagt tctggtagag aaattccaga
     7201 atataatcaa ttgaaaaaga ttcctgttaa tataggcttg cttcctattt taagagtggg
     7261 atctctttgg ataaaaggaa aacttcaaaa caataatgca gttacagata tagccactct
     7321 taaaaatatt gaaataaatg caaatacagt agaaataatt aatagcaatt acaaaataaa
     7381 atatttagat tcaagcggaa gagaacaaga gtgcaaacta ttgttgcaga aaaattttgc
     7441 aacggatgca aaagtacctt tttcttgctt caaaattcaa aatgaaaatt acaaaaatgg
     7501 aataattatt ccagtaatag aaattatccg tttttactat gcctgttcaa ctagactagc
     7561 tcatttaaca ttttctaatc cagacgaaat tacaaaaatc tcatatgcga tagataatga
     7621 aacagacaag gtagtcatca aattaccaca gcaatataca gatcaagaag cctttatttt
     7681 agcaagaata ttgacttcaa atattgcttt atctggactc tttcagatta aaaattccct
     7741 aataaaaata ggagttaatg aacaaccaac tacattatta tctaccaatt tcccatttat
     7801 caatccaacc aatttaatgg ttcgcggtat taaaattgtt gatagattct tagttacgga
     7861 gatttgttct tgttcggctc cttttcccga tatttctgtt gataggaata atgacaatcg
     7921 aaagggagat aaaaaaacag atatacctga tgataaaaag aaagaatatc gaagaggggg
     7981 acaaactcct agtctcaagg gaaattccac tattcaaaat caatatgaat cagtcaattg
     8041 cgtatcaaca ttacaattta acttaaacat aaattgcttt tcagatttga tcggtaaaaa
     8101 gattgaaaaa ccccaaaagc cctttaatga atacaaagca cagaaacttg ccataaatga
     8161 aaaaattaat gtaactagct taggagtgga tttaggtaca tggagccaaa ctacaattgt
     8221 gcctgcgtcc attagcactt ttaataccca atctgaaaaa caaaataggg atgccttagc
     8281 tgcgactttt gacaattccg tacgtgctat tgaacatttg aatgaagaat attcagatat
     8341 tcaggcaact atctacatgt ctagctctcg agcttgtgag tttgttccat taactgactc
     8401 agcccataaa gcccaatggt cgtatcttgt ttccaaaacc aaaacaaggc gtaaggtatt
     8461 attaggaaaa attgagtaca aagataaaac attttggttg attgaaattc aagctcggaa
     8521 aagtgagcat tttgttacgg cgattattta ttttgaggca attgacgaga caaagtattt
     8581 gactcaatta ttatatgtct tagcaaaaca gcggggaatt tgggataaag ttccaaaaaa
     8641 cttcagtttg caaattatta ctctaaagca tacgcataaa gatatcaaga gctacgctca
     8701 aaaaatacac aaaaaaatat caaattttat cttaaaataa attagttagt aatataacta
     8761 atctagttat aaattaccaa aatacttcag gctgatactt ttatataaaa tttaagttac
     8821 ctactctctt attgataagg ctggtggtgt cgtaactact atataggagt aatggttccc
     8881 atctgctaac ataaatgtta aggtaagagt aaattctaaa gacacaaaag aaaacggttc
     8941 ttttgatatt tcaaggttat gtttttaatc tataacataa tattaacaat ttctattttt
     9001 aggtattcaa aatgaaaaat cgagatactc cgcataaaaa atttatcaac cctacggaat
     9061 atagtctatt tgcagaagaa gaacaatatg attttgaatt aatgattgaa aatcagataa
     9121 aagaacatga aaaagaaatt gattactatg taactgatta tcccatagaa acactagttg
     9181 ctaaattaga tcgacaagaa ttgtttattc cagactacca gagaaaaaac atttgggaaa
     9241 aacatcgtaa aagtaggttc attgagtctg ttttaattgg cttacctatc ccatttttat
     9301 ttttttggca ggatccttca actggaaata tggaagtggt agatggtgca caacgactaa
     9361 cagcattgtc tgaatttatt catggcaatt taaaattatc ctatttggaa aagcttattt
     9421 atcttaatgg ccaaacttat caccaactcc tagaaagtcg tcaaaagatt ttcaaaaata
     9481 aacctatccg aggcgtagta cttagtaata aaacagacaa agaagctcga cttgacttat
     9541 ttgaacgtct taatacaggt agcaaaaatg ctacacctgc agaaattcgt cgaggtctgc
     9601 ttcaaggagc attttacgat atggttgaga ctttatctga agataaaaca tttaatgaat
     9661 tagcaaaaat tagcaagaat atgcaggata tgggggagcg agaagaattc attactcgct
     9721 tttttgccta tggtgagtat ttagaccatt attctgctaa tgttcgtaaa tttatctttg
     9781 attatacaga aatcatgaat gaagaatttg ctaaaaatcc aaacatgcgt gcgacttacg
     9841 aacaaaaatt ccatgaaatt atggcttttt tcaaagaaaa atatccaatt ggttttaaaa
     9901 gagatggaaa gggtcagtct tctcgtactc gtttcgaagg catggctgtt tcagcttatc
     9961 tcgctgaaaa ggaaaatcca ggcattttag ctcaagttac cccaaaggag tgcgttgata
    10021 tttttgacag taaagaattc attgaagtaa ctggtagtga tggtgcaaat gccactaaaa
    10081 agctacatgc tagattattt tttgctaaaa atgcctttaa acggaatcat ccatgaactc
    10141 agtaaaatct atgatggaaa gtcgtgaaga ggaaataaac ctatatatgg atctgctaaa
    10201 gtatatccat gatagtactc aacatggttt accagcttta caaaatagaa atggtgttga
    10261 atatcaattg gaaaatactc atctccatat ccttaccgct agtatctata tgttactata
    10321 taactacatt gaggctgttg ttacccaaag cctaaatact ttaatttgct atattcaaaa
    10381 tgaaaggatg cctattaccc aattttcaga acccatccaa aaagaatggc taataggagg
    10441 gttaaacctt aatgatgcaa aagctagtgt agaaacacga ttaggtaatg gactcaaaat
    10501 gcttaaacat ttcggtgata cacaaaaaat agatattaag atttctaaag gaggaggaaa
    10561 ttttaatgat gacgaaatat ttaagcttgc aaagaaactt caagttaata tgtgtatcga
    10621 tgacgaaact caccgccaat tacggcaagc aggaaatatt acctatgaaa gtacgcgcaa
    10681 atttcaagat acttgtatga aagaaataac ccgtgaacga aataaacttg ctcatggaga
    10741 aatcaccttt caagaatgtg gtagaaaccg aacaatatct gatttaatgg caataaaaga
    10801 cactactttc cgctatatgc atttatttac tgatgcattt actgaatatc taatgcagaa
    10861 gaaatatctc aaaccaacag tttaaaacta tttccgattg cctcacctag caatacagga
    10921 acagcattac caatcattcg actaatttgg cgaattggag tatcagtttc ttgagtgaac
    10981 tggtaatcta gaggaaaact ttggaataag gctgcctccc ttaaagaaat tgcacggtct
    11041 tgttccggat gcccaaagcg tccattgccg aagccaatac ataaagttgt cattgttggg
    11101 gcgggcttgt cccattccat acgaccataa attcgtttat aagactgtcc tttttttttg
    11161 cgatgacatg cggctcgtaa atcttctggc caatccatcc atgttcctcc tggacgagaa
    11221 gcacgaatcc gttctagatt acgagctgac attgatgaac accgatgtaa tacatctata
    11281 gtatcttgtt tgcctgctgc cagtggcggc aagtgtccaa tagcctgacg aactgttaca
    11341 tggcaatttg aatgcgttgg aggaaccatc tgaatattac cgatacgtga tgcaagtaat
    11401 acgtgtctac gtcgtgattg agggactcca taatctgcac ataatacttt ttcagcccaa
    11461 acttggtatc cctgttgttt taaattattt acaaaatctt ggtatacctc atgttttaca
    11521 acatctggca cattctccat agtcacaagc tctggttgca cttcatgaat caagcgagaa
    11581 aaagcgtata gtaatggcca ttttttgtca ttacgaacat cacgaccttg attgtaagta
    11641 gaaaatggct gacaaggagc acaaccagct aataatctaa ctgcatcttt tgaatagtgc
    11701 gcctgaatct gatctccagt aactgtttcc acatcttgta atataaattt tgcaccttta
    11761 ttgttatact cgtacgcata tgcacaagct gcatcaatat catatccagc tttcacttta
    11821 attcctgctc gaagtaaacc tgctgtcaac ccacctgctc cacaaaataa atctactgct
    11881 tcaattttca tttttcaact catatttttg atataaggat tatatcctta actttgattg
    11941 gaatcgagct tttaatagta tataagctct gaatagtaca gttccttatg aacgtttcta
    12001 ttcattttta aaattatttc ctgaaaactt tgggggcaat cattaaaatc cttcaataaa
    12061 tggttataac ggacggcttt atcttgccat gctttaaata gattttttgg aaaattttgt
    12121 attaaccaca aacaagcatg aatagtactg tgtcgttccg ccaaactgcg actttcccaa
    12181 ctcagtcgac gagaacgttc taattgaact ggaatctgat gtatttgctt gcacaaataa
    12241 gagtacaaat ccggtgcaag cttctttgat gtagtaatcg ccaaaaagtg atgcagaatt
    12301 tttagttgtt ctttttcaga ttggttaatc agtagctaga catagcgggc aataaacaat
    12361 agcattatta tcgcgtttac ggtgattggc tttaaggctg tttatccacg tcaattgtcc
    12421 tgaaaaattg tttttatcaa ataatcgacc ttgtaggcta tctaatgtgg ttgttttgat
    12481 tcgaatggtt gaacagcctg ttttttcagc caatgtattg accaaccagt caggagtatg
    12541 gcgatctata tccctattcc atatctcata attaggaaat agcaaatgac agaaggtttg
    12601 tactttcaac ccgtttgcat gtgctaagcg aaccaaccat gatgtaaaca attcatcagg
    12661 attcggaatt tgtaattcat ttcctaagta tttaattaca tttaaaaacg ttttctgctt
    12721 attcaaactc ccggaaagga tatggtggat ttcatcaata atcaacattt tagtattaac
    12781 gtatttgagc aaatgaagca cttgggcaag ttttttatct gtcctgtcat gagttttata
    12841 gggggcaaac agtaaatcta aaatagcatt gtaaaaacgg ctttcatcag gtataggcgg
    12901 tgcttgaata atcactactg gtgcgataat accatttcca tgtggattat catgggctgg
    12961 atgttgccgc aaaaaatgtt taatcaacat ggttttaccg ttattagtat caccaaccaa
    13021 caacatatta ggcatacggt tttgtttcgg gaaacttagc aaatcttcca gttttaccaa
    13081 aacctgttga gcctgcggat aacctatcca acgaggggaa cgtatgtatt cgatgcgctc
    13141 aatatcgcta tactttagtt gttcttttgc tttgggagaa aggtgaggat aattcatgat
    13201 aaatcgtcca aatcatcaaa aggctgaata tcatcaaaca tattttcctc attttcagac
    13261 gatataatat tcctcgaatc aatttgagac ataggtaaag atgctcgagt acgttgatct
    13321 aattggcgac gttgttgagc gcgacggata gctttagtct taccttgagc ttgctgttcc
    13381 aattctcgca ttcgtccgta tgctgtaaaa atcatgttct catcaatatt ctgtttctgc
    13441 tcatttttta attgtctctg aatctctcgt aactcccaaa tgctgataac tggattcgaa
    13501 ctgttccgat aaggtattgg aaaatacatc gataaatctg ggtcataaaa ccaaatcacg
    13561 cttaaatcac gtggatctat gcgaaaaatg aactggcgag cctgtctagg attagtcgga
    13621 ttagttgctg caatccatgg tcgtaaaacg tcatgccaat aagtgatttt atggataact
    13681 accccatatt gctgcaccgt tcgcatttca aacggcataa aggtcaagcg caatagtgtc
    13741 tcatcaacca ctttatcagg taatcctgtt cctaaagaaa catcatctcc taatacacct
    13801 gatttccatt tggctaacgg tgagatcccc aatccagaat gagtacgctg atgataaaca
    13861 tcacaaataa atagtgttaa ccacgtttca aattcagtta gcgttaatgc cgccttgctt
    13921 tcggaagcat aatcttttct ctgttgtgta tttgcaaaag tagtaccagt taaaccgtgg
    13981 acttcatcca aaatcgtacc tagtaaacgc tcaatgtgtc ctccgaaatg aggacgtgcc
    14041 acaggcctcc actcaatttc aataccgtat tgtttacacg ccttttccag cactttgcca
    14101 cggaattctt ttgcattatc caaatgaatt ttgcgtggca aaccccacac aggccaccgc
    14161 cctttcactc catgttttgc caaccagctt tccttagaca aaatactatg tgctaaacac
    14221 aaacctgtag ataatgcact tggtggatcg aatgacacat aaaatcccgt aaccattcga
    14281 ctgaacacat caatcgccag cgtaatccac ggcctaccaa taggcttacg atatatatcg
    14341 tcaactaaaa taatgtctaa ctttgtatga tctatttgta ccactgaaag cggataatcg
    14401 gcatttggaa agctaccttg taaaggagaa aatttcattt ctgctgcttt atggcttagt
    14461 cgtttagcaa ttttaatttg aggcgcaata tttttgattc gagaacgaat ggtattagaa
    14521 agataatcaa gattagcaat ttggtttaac caattttgcc attgcagcct gatttcatca
    14581 ttaaagaaaa taacattctc acaaggactg ttgctcaagt catattgaca attccaacaa
    14641 tatttcaact ctgtaaagcg ctgcaaatta ggtttaccta aatcttgtcg gtgaaaagca
    14701 atatatatgc cacatttagg acagcaatca tgcatcatga tttgatgtat tggacaaaat
    14761 gtatataggg ctatgcgcca tgattttcgg aaatatgttt catggcatga ggagctggta
    14821 aattttgttt atggcaacgc catgtaattt ctctaatgat tttctgtatt gatttacgtt
    14881 gtgtgcttaa ataatccact tccaatactt gcctgataag ctgttctaca tcatcagaaa
    14941 gctgtgtttt accactatca ctacgctgct tacgtgttaa agcagtcaga acgccgcttt
    15001 ccttataact ttttaaccag cgataaaccg tactgatatg tacaccgcat tcggcagctc
    15061 tcgcttgaac atcagattta ctacgttttg gatttcggat aagtggctca attacatcga
    15121 aatgttgttg agctaattgc catttttgct caggaatact atctaagtgg gttaattcag
    15181 cactctggac tgttgataga tccgctaatt ttgcttggta atctgcaccg gttacactat
    15241 cgcgtaactg atatgtttct aactccagga ctgctgtaat tacacagatt ttgttttgaa
    15301 acaaaaccat tgcaccgaca ttcacattta aagttgcttt cattttttaa accatatagc
    15361 ggattgcata gtgagtggtt gatttaaatc tgcgccaatt tttcgttctg caattaagta
    15421 ccacaataca ggcaataatt ttgcttgatt gtattcatcc tgaaaagtct gttgaataag
    15481 ctgtttaggt gtggattttt tcaattcgcg taacgattca tccaataaag tcataaaggt
    15541 ttcggatgag cgggtaaaat catctgagtt tatccaattg acgtttacgt tggcttggac
    15601 taatccagtt gattgaattt aagattgttt cgttaataca ttcagtttgt tgttgaatgg
    15661 cggctacagc agcttgtgtc agcaaacgag aaagctctcc aatatatcct tcactcatgc
    15721 catataactt tgtagccaac gaaatttcat gtaaatccga cggctgacta agcggcagca
    15781 tccgttcaaa gctcatcaat aaacgcataa aatccttatt taattcccaa cgaggaagca
    15841 ccgcaggttc aaaacggttg gccaactgtg catcagtttg aatggctcga tatgcgtctt
    15901 taatacctac acctacaata tgcttcgctg tattcgccag ataagccatt ttgttgatat
    15961 ggcaataaaa agcgtacatt gcataaataa tcagtatgaa tctctaactc tgtaatcagt
    16021 ttaaaatgcc aaccgtgtaa tttgcaaaaa tgtaaggcag cctgaaattt aggtttgagt
    16081 tcacaccagt ttttacgtaa atcgctacga tattttactt cgtaaataat tacatcattt
    16141 tgataatgaa ccaaaacatc aggggtatag atatgcttct ttcctgctgg gtcaaaccac
    16201 tcaatcgtga taggctgtac ttcgaaatgc tgcacattag aatcaaattc cagcagagtt
    16261 aaaaaatcac gctccaaaga tgactcataa gctgcaatgc ctatagcttt gttatatgga
    16321 gcgatgcctg taatatttcg atagttttta ggaattttac ggattggcat agttttatat
    16381 gtgtagtaga aaaaggatta aaaggaagac aatcttaatg agttaatgcc ctttatcaat
    16441 aacccaaact caattagctt tcttataatt tagtctcaaa taacaatatt attttcaaat
    16501 cttaatgcaa aatttttgct ctcagtcgca aataacgtta ttagaagtat aagattttgc
    16561 atattctagt cgcaaacaac gttggaattc acagcgaaag ccacggcggt tgcgggcatc
    16621 attaaacccg gaacaccgcg ccaaagtgaa gaacgattcc ctgcgcctgc cgcgccgttt
    16681 gatgtaaaca acatcaattt gaattgtcca tgccgtctga aagcccttca gacggcatga
    16741 tttattccca aaatccgata atctcaaaag aatctgtcag ttaatgagaa cgattcatta
    16801 taaattttga taaaaaaccg tgctctattt gttttttgct atggttaata agtgtaaatg
    16861 ttctttattg atgagatgga ttcttagttt tagcagcaaa aaaaaacgat aagaaacaga
    16921 gcattgattt ggaaggcatc ggggaatacg ccctatttct tttgataatt gtttgtattt
    16981 taaatagaaa atttgtcatg ccgacagctt ttgcatacaa tgcacgcatc ccgaacagtg
    17041 cttcatccac tgtttaacca cacgaaaccc aaattaagaa aagagaaata ttatgcaagg
    17101 caatcaagca gttttagatt acatgaacga attgctgtcc ggtgagctgg cagcacgcga
    17161 ccaatacttc atccactccc gtctctactc cgaatggggc tacaccaaac tttttgaacg
    17221 tctcaatcac gagatggaag aagaaaccac acacgccgaa gactttatcc gccgcatcct
    17281 gatgctgggc ggtacgccga aaatggtacg cgccgaactg aatatcggca cggacgtggt
    17341 ttcctgcctc aaagccgacc tgcaaaccga atatgaagta cgtgatgcgt tgaaaaaagg
    17401 catcaaactg tgcgaagagg ctcaagacta tgttacgcgc gacctgatgg ttgcccaact
    17461 gaaagatacc gaagaagacc acgcacactg gttggaacag cagctgcgcc tgatcgagct
    17521 gattggcgaa ggcaactact accaaagcca actgtaagct gtattcggaa taaaaggagt
    17581 ccgtatgaaa ggcgaccgtt tagttatccg cgagctgaac aaaaacttag gcttactgct
    17641 gataaccatc aaccaatatt tccttcacgc ccgtattttg aaaaactggg gctttaaaga
    17701 actgggcgaa catttcttca aacagtccat cgttgaaatg aaagctgcag acgatttgat
    17761 cgagcgcatt ctgttcctcg aaggcctgcc gaacctgcaa gaactgggca aactgttgat
    17821 tggtgaatct accgaagaaa tcattgcctg cgatttgacc aaagaacagg aaaaacacga
    17881 agccctgctt gctgccatcg ctacagcaga agcgcaacag gattacgtca gccgtgattt
    17941 gttggaaaaa caaaaagaca ccaacgaaga acacatcgac tggcttgaaa cccagcagga
    18001 actgatcggc aaaattggtt tgccgaacta cctgcaaaca gcggcgcaag aggactaaaa
    18061 cacaaaccac tgccaaatat agcagtcccc tgcatcatca gacggcatat ccgtataaac
    18121 gggtatgccg tttttctttg ccggtttgcg aatcaaatct aaattgattt gaatcaatac
    18181 tcgataacgg tttttgtttg ataataccga tatgaaatct agattgcttg tttgctgttt
    18241 cccaatctgt cttgatttta cctctttcct cttgatgtgt gtgtgtttgg gtgtggctgc
    18301 cgccacccct ttttttggct tttatgtgaa gtaaaatccg taacagcaaa tgcttaactt
    18361 taaccattta tccggaaaca taatgatggg caacaaattg actctgcctg ccgaactgcc
    18421 tgacgaacag gatttgcggg cggtattggc atacaatatg cggcttttcc gcgtgaacaa
    18481 gggttggtcg caagaagaat tggcgcggca atgcggtttg gacaggactt atgtgtcggc
    18541 agtcgagcgc aaacgctgga acatcgccct gtcgaatatc gaaaaaatgg cggcggcttt
    18601 gggcgtggcg gcgtatcagt tgctgctgcc gccgcaggaa cggttgaacc tgatgaccaa
    18661 ttccgccgat acccgacaaa tgccgtctga aagcggtatt tgaccgccga accatccacc
    18721 tcccttttcc cgccaatcga aagaataaaa atgcctgcaa acctgtcttc agcactggaa
    18781 accttcaaac ggcagcgcga tgccgccgaa gcgcattatt tgaaagccaa ccgcgtgtcg
    18841 gtatttttca gggaatatac ggcggcagtc gaaaccttac tggcggcatt gtgggtagaa
    18901 cattttcaaa acagcgcgtt atgcctgatg gcggtgggcg gcttcgggcg cggcgaactg
    18961 tatccctgtt cggatgtgga tttggcggtt gtctcccctg ccccgctttc agacggcatt
    19021 caggaacaga ttgcccggtt tgtccaaacc ctgtgggact gcaaactgat gccgtctgta
    19081 aaaagcggca gcgttggcga actgtgcgaa agcgtgcgcg acgatattac gggggacacg
    19141 gcgtttttag aggctaggtt tttgtttggc aaccgccaaa cggcggataa actagcggaa
    19201 aaaatgaacg cgcagcgcaa tgtggcggcg tttgtcgagg caaaactggt ggagatggaa
    19261 caccgccacg ccaaatcgca aggctcaggg gcggtattgg aaccaaacat caaaagctgt
    19321 ccgggcggtc tgcgcgacat ccacaccctg ctttggatag cgaaggcgca aggcttggcg
    19381 accgacctgc ccgatctgct caaacagcgg attttgacgc gtgccgaagc cggtatgctt
    19441 tcgcacggct accgccgcct cgcccacatc cgcatccatc tgcatttaaa cgccaagcgc
    19501 gccgaagacc gcctgctgtt cgatttgcag ccgcaagtcg ccgaaagtat gggttatcaa
    19561 ggcttgaacc tccgccgcca aagcgaagaa ctgatgcgcg tgttttaccg cgcgattaaa
    19621 accgtcaaac aactgggcgg catcctcacg cctatgctgc gaagccgcgt ttcctccgcg
    19681 ccgatgcacg ttaccctgcg gattgacgac gactacatcc aagtcaacaa ccaaatcgcc
    19741 gcgcggcaca ccgatatttt tttgaggcag cccgaacaca ttttcaaaat cgtcgaaatt
    19801 atgcagcagc gcaacgacat taccgcgctt gaaccgcaaa ccctgcgcgc ctggtggggc
    19861 gcgacgcgca aaatcaaccg cagcttctac caaaatcccg aaaaccgccg ccgcttcgcc
    19921 ggttttttcc gcaacggcaa cgggctgacc cagaccctgc gctttctcaa cctctacggc
    19981 gtgttgggcc gctacctgcc cgcgtgggaa aaaatcatcg gcctgctcca acacgacctg
    20041 ttccacatct atcccgtgga cgaccacatc ctcaccgtcg tccgcaacgt ccgccgcctt
    20101 gccttggata tgcacagcca cgagctgccc tacgcctctg cactgatgca gtcctttgaa
    20161 aaacaagaca tcctctacct tgccgccttt ttccatgaca tcgccaaagg acgcggcggc
    20221 gaccatgcca tacaaggcat cgcagacgcg cgccaatttg ccgctgacca cttcctgacc
    20281 ggagaagaaa gcgacctgct cgcttggctg gtcgaaaacc acctgctgat gtctgccgtc
    20341 gcccaaaaag aagacatcca agaccccgac gtactcgatg ctttctgcaa acgcgtccaa
    20401 acccacgaac gcctcagcgc gctctacctt ctgaccattt ccgacatacg cggtaccaat
    20461 cccaagctgt ggaacgcatg gcgcgccagc ctgctggaaa gcctcttcca tgccgccgga
    20521 cgctacctta caggcaacgg cggcaacccg cacaccctct tcggccgccg ccggcaggaa
    20581 gccgccgact tactcacccg cgccgccgtc cccgaaaaac agcagaaaaa actatggaac
    20641 gcgctcggtt ccgcctactt cgcccgccac cagtcccgcg aaatcctgtg gcacgccgcc
    20701 aacctcgtac acgattttga aacccccatc gtccgcagcc gcatcctgcc ccaaagcgac
    20761 agcttccaag tgatggtctt tatgcccaac ggcccgcgcc tgttcgcccg cctctgccgc
    20821 atcttcagcc gccacggctt cgacattctc gccgcccgcg ccttcatcac cgaacacgac
    20881 tacatcctcg acaccttcat cgtgcaaatc ccctcgcagc acgctcccga agactacccc
    20941 gacattcaaa gcgcgctcga agccgaactc aacagcttta tccacggaca caccgttgcc
    21001 gaaacctcca gttacagccg ccgcatcagc cgccgcagcc gctatatgcc gatagcgcca
    21061 agcatcacca tcacccccga agaagactat ccagactggt actccgtcga aatcactgcc
    21121 gtcaaccgcc ccttcctgct cgccgatatg gcggaagtct ttttcgccca caacgtcagc
    21181 ctgcgctacg ccaaaatctc cacgctggac gaacgcgccg aagacagttt taccgttttt
    21241 agcctagacc tgaaaaaccc aaaaatccag tcctcattga agcaggcttt gctggaacag
    21301 ttgacataag caccctgccg gttttcagac ggtatcccgc cgccgcgccg tatgccgtct
    21361 gaaagcgggg gatattgcaa acaatttatt aacataatga tttatatgaa tattattatg
    21421 gactcaacaa gaaaatttgg gattttatgg gaataaaact ctgaatgtaa tggttttatt
    21481 tatggaaaaa ttcagataat aataggagag aatatatatc ctaagatatg cccatatgga
    21541 tattttacgc taaatacagt ttttaatagt ttgaaatcat cctttgaaga gaagtattat
    21601 gcaggtggaa acaatggttt agattttgga gagcaactat ttgatataga taaatataat
    21661 tctttggaat tatgcaatat attttctatt gatactacat atatgagtgg agggggtaat
    21721 tgtgaaatag attgtttagt attagaaatg ggatattctg gtgaggaaga gagacttttt
    21781 tatagttttg acaatgggaa aaactttaaa gaaatcaggt ataagaaagg tacagttgaa
    21841 tctgttattt ttcaattgaa tctataatat atggggctgt aactagatta gcagatatgt
    21901 taccctcgaa atatcaagat aatatactga aaattaaagg aaaaaataaa taaagaacta
    21961 ctccgttttt ttgtgctgga agttaccgcc cgatataggg cttcaataac tgagccactt
    22021 accaaaaatt agaaaaaagc ttttcctgaa attgaaagaa cgggagcaac agtggtcggc
    22081 aaaggaaaac cgggctattc aaaggggacc aaaattaaac ctgataaact gtcaagccgc
    22141 cggtaacgtg ctataatgcc gtgcatcttg cacaaactga aagaccgagc attatgcgta
    22201 tcgtagaaaa agcctatact ttcgacgatg ttttgttggt tcccgcacat tcgaccgtgc
    22261 tgccgcgaga cgttaaactt caaaccaagc tcacccgcga aatcacactc aacctccccc
    22321 tgctttccgc cgcgatggac actgttaccg aggcgcgcct cgccatttcg atggcacagg
    22381 aaggcggcat cggcatcatc cataaaaaca tgccgcccga aatgcaggcg cgcgccgttt
    22441 ccaaagtgaa acgccacgaa agcggcgtgg tcaaagaccc cgtaaccgtt gcgccgacga
    22501 cgctcatccg cgaagtattg gaaatgcgcg cgcagcgcaa acgcaaaatg tccggcctgc
    22561 ccgtcgttga aaacggcaaa gtcgtcggca tcgtaaccaa ccgcgacctg cgttttgaaa
    22621 accgcgtcga tttgcccgtt tccgccatta tgaccccgcg cgaacgtctg gttaccgtcc
    22681 ccgaaggcac aagcatagac gaagcgcgcg aactgatgca cacgcacaaa gtcgaacgcg
    22741 ttttggttct gaacgaaaaa gacgaactca aaggtctgat taccgtcaaa gatattttaa
    22801 aaaccaccga gtttcccaat gccaacaaag actccgaagg ccgtctgcgc gtcggtgcgg
    22861 cagtcggcac cggcggcgac accgaagagc gcgtcaaagc cttggttgag gccggcgtgg
    22921 acgtgattgt cgtcgatacc gcccacgggc acagccaagg cgtgatcgac cgcgtgaaat
    22981 gggtgaaaga aacctatccg cacatccaag tcatcggcgg caacatcgcc actgccaaag
    23041 ccgcattgga tttggtcgcc gccggcgcgg atgccgtcaa agtcggtatc ggtccgggat
    23101 cgatttgcac cacccgtatc gtggcaggtg tcggcgtgcc gcaactgacc gccattcaca
    23161 acgttgccga agccctcaaa ggcacgggcg ttccgctgat tgccgacggc ggcatccgct
    23221 tctccggcga catcgccaaa gccctcgccg caggcgcgta cagcgtcatg ctcggcggta
    23281 tgtttgcagg cacggaagaa gcaccgggcg aaatcgaact ctaccaaggc cgctcgtaca
    23341 aatcctatcg cggtatgggt tccttgggcg cgatgagcca aggttctgcc gaccgctact
    23401 tccaagacaa aaccgacagc accgacaaat acgtccctga aggtatcgaa ggccgcgtgc
    23461 cttacaaagg cccgatcgtg aatatcatcc accaactgac cggcggactg cgctccagca
    23521 tggggtattt gggttgcgcc aatattgctg aaatgcacga aaaagccgaa tttgtggaaa
    23581 tcacttccgc aggtatgagc gaatcgcacg ttcacgacgt tcaaattacc aaagaagcac
    23641 cgaactacca tcgctgattt gaacagcttt ttcaaggcaa aatgccgtct gaaacctgat
    23701 tttcgggttt cagacggcat tttttgtccg ttcaaggcaa gccgcacggg ctgattgcgc
    23761 ggacgggcaa agcaattcgg acggacacgc cgaaagaatg aaaatgccgt ctgaacgccg
    23821 tttgcgccca gtctgccgaa taccgccggc ctttaaaacg gcggtggaaa taaacgcaat
    23881 ctttcaatac gggcgtttcg attggcctga aatgatgcgc cggccgccat atcaaaaaat
    23941 cgggcggcgg cttcgggaaa gtatgccctg ccctgccctc cccaagccgc cccgtcagca
    24001 ttatgatttg cctttccgtt tttttacctt ttgtacttca atcggaatcg gtttctttgc
    24061 ctttttcccc gattctgcgg cggcagacgc gccccttccc cttactttgc cgcctcgggc
    24121 tgtttttttc ccggcggcgg ttttcggctt ccctttcccc gccgtccctg ccggtttggc
    24181 agacgcggat gatttaactt tccgccctct gccgctcccc tcggcaatca ggacaaaatc
    24241 gatttttcca tcatccaaat cggcacgggc cactttaatg gcaaccctgt cgcccatatt
    24301 gaaacggatg ccactgcgtt caccttcgat tgccatgatt tcggggcgga agttgaaata
    24361 gtcttcgccc aaatcgctga tatgcaccag accgtcgata tggatatcgt ccaaagtgac
    24421 aaatattcca aaatttgcca ccccggaaat tttgccttca aatatttcgc cgactttgtc
    24481 acgcatatag taggttttca gccagttttc cacgtcgcgg ctggcgtcgt cggcacggcg
    24541 ctcacagaac gaggtatgca cgcccaaagc ctgccagctt ttttttggcg tgtaggtttg
    24601 ctgattcaac acggctttga tggcgcggtg tacggtcagg tcgggatagc ggcggatggg
    24661 cgaggtgaag tgggcgtatg cttcgtaggc aagaccaaag tgtccgtcgc aatgcggttc
    24721 gtaaaccgcc tgttgcatgg agcgcaacat catgacttgc agcaattcgg catccggcct
    24781 gcctttgaac tgtccggcaa gcgcggcata gtctttcggc gacgggttgt cgccgccgcc
    24841 aagttgaagc cccaacagac cgaactgctc gcgcaaggcg gcgagttttt cgggcgtggg
    24901 ccccaaatgg ttgcggaaca atgcggtatg cttgtttttc aacagaaaat ccgctgcgca
    24961 aacgtttgcc gccaacatac attcttcaat cagcttgtgg gcatcgttgc ggacaacggg
    25021 gacgattttt tcaattttac cgttgtcgtc gaaaagcatt tgggtttcga tgctgtcaaa
    25081 ctccaccgcc ccgcgttcga aacgcttttt ctgaaggatt ttgaagagtt tgtaaagcgt
    25141 gtcgatttgg gctttgaacg gatgcccgat gccgcctgaa agccatttcc aaacttggtt
    25201 gtaggtcagg cgggcatgag agcgcatcac ggcggggtag aagcggtatt ctttaatatt
    25261 gcccgcgtaa gtgataacca tatcgcacac catacacaaa cgctcgacat taggattgag
    25321 cgagcagatg ccgttggaca ggttttccgg caacatggga atcacgcggc gcgggaagta
    25381 aacactggtg ctgcgttcct gagcgtccgt gtcgatagcg tcatcggggc ggacataatg
    25441 gctgacatcg gcaatcgcca cgaccagacg gtaattgcgt ccgatttttt cggcaaacac
    25501 cgcatcgtca aaatctcgag ccgtttcgcc gtcaatcgtt accaaaggca ggtcgcgcaa
    25561 atcgacgcgg cctttcaaat cgcttttgcg tacatggtcg ggaatttttt tcacggcttt
    25621 ggcacacgct tcactgaatt ggtgcggcaa atgatgcttg cgcacggcaa tttcaatctc
    25681 catgccgctg tcggcataat cgcccaaaac ttcgatgatt tttgccactg ccggccggtt
    25741 ttgctcagga taaacctcaa tttcgccgac gatgacctga ccggattcag gtttgaaacg
    25801 cgccacgccg tccggttcca atacgatgct ttggttcaga cgcttgtctt ccggctccaa
    25861 aatcgccacg cccctatcca tatagaaacg gccgaccact ttgctttgcg cgcgttcgac
    25921 aatatccaga actgtccctt cgcggcggcc cctacggtcc atgccggcag gacgaacagt
    25981 gacaatatcg ccgtgcataa tgccgcgcat ctggcgttca tacaaaacaa aatcaccgtc
    26041 tttggcgggc gtgagcggca cggcaaaacc gaaaccgtcc ttatgcgcct cgacgcggca
    26101 tttgaccaaa tccaatttgt ccgccgcgca aaccgcgccc cgacggttga ttaaaacctg
    26161 accgtcccgc gccatcgcct tcagacggcg ttcaaaaaag acatactcgt cttccgtaat
    26221 cgacagttcg cgcgcaagcg attcgatttt tgaaggcaca cctttgcgct ccaacaattc
    26281 gattatccat tcccgactgg gcaaaggatg ttcataacgc tgtttttcac gacttaaaaa
    26341 cgggtctttt tcccgtaaat ttaaagattt aatattttta ttcatttgga ctgttgacat
    26401 tctctttatg aactatataa tagcgacttc tttgcggcag gacactgtac cgcaaaacag
    26461 caaataaagc aaagcccggg tggcggaatt ggtagacgcg ctagcttcag gtgctagtat
    26521 cctcacgggt gtggaagttc gagtcttctc ccgggcacca aatcaaatgc tttgctttat
    26581 ttcaatattt agattgcccg ggtggcggaa ttggtagacg cgccagcttc aggtgctggt
    26641 atcctcatgg gtgtggaagt tcgagtcttc tcccgggcac caaataataa attatcccga
    26701 ttgggataat ttccgccatt agagaggtgg atgagtggtt taagtcgcac gcctggaaag
    26761 cgtgtatacg tgaatagcgt atcgagggtt cgaatccctt cctctctgcc aaatacaaat
    26821 cccaaacttt tacggtttgg gattttttat tattcaaaca ataatttcat tgggaaaacc
    26881 cgttattgtc tataatcgtc atcaaccgct tacaactatc cgttaccgtt ttttttatgg
    26941 tatgttttgc ggtatatttt atagtggatt aacaaaaacc agtacggcgt tgcctcgcct
    27001 tagctcaaag agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta
    27061 tctgtactat ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatatcctct
    27121 tcctaagctt gtaaggactc aaaaggcgta ttccttcaaa cggcattgtt cgacatgctg
    27181 ccgggaagcc gaacccgata ccgaaacgcg acggggcgat ataccgctgg aaaaatggcg
    27241 gcagtttctt gattgggtag gaaaaacgcc gtctgaaaca gcttcaagaa gccgcaaagc
    27301 ccgtccgaat aaaaaatgcc gtctgaaatt tttcagacgg catcgcgtat catttaaaca
    27361 ttaatccagt aaacatcaat ccagcttttt aaagtggcgg cggcgttcca attcgctcaa
    27421 gtaacgcttg cgcagacgga tggattgcgg cgtgatttct accagctcat catcgtcgat
    27481 aaactcgacc gcaccttcca gcgtcagctt aatcggcgtg gtcaggcgaa cggcttcgtc
    27541 ggtaccgctg gcacggatgt tggtaagttt tttgcctttg agcgggttga ccaccaaatc
    27601 gttgtcgcga ctgtggatgc cgataatcat accttcgtag attttgtcgt tgggcgatac
    27661 gaacatacgg ccgcggtctt ccagattcca caaggcgtaa gcgactgcct cgccctgctc
    27721 ttgggacacc agcacgccgt tgtggcggcc gggcatatcg ggtttgacgg gcgcgtaatc
    27781 gtcgaacacg tggctcatca gcccgacccc gcgcgtcagg gtcataaatt cgccttggaa
    27841 accgatcaag ccgcgcgctg gaatatggta ttcgaggcgg gtgcgtccgt tgccgtcgct
    27901 ttccatatta gtcagttcgc cacggcggcg gccgagttct tccattaccg cgccttggtt
    27961 gtcgtcgggt acgtccacgg tcaggttttc ataaggttcg catttttgac cgtcgatgtc
    28021 gcggtacacg acgcgcggtt tgccgacggc gagttcgtag ccttcgcggc gcatattttc
    28081 caacaaaatg gtcaggtgca gctcgccgcg tccggatacg cggaacacgt cggcatcggc
    28141 ggtgtcttcc acgcgcaggg cgacgttggt cagcaattct ttttgcaggc ggtcgcggat
    28201 ttggcggctg gttacgaatt tgccttccgt acccgccagc gggctggtgt tgaccataaa
    28261 gtccatcgtc agcgtcggtt cgtccacgct caacatcggc aggcctttgg ggttgtcttt
    28321 gtcggtgatg gttacgccga tgccgatgtc ttcgataccg gaaataatca cgatgtcgcc
    28381 ggcttcggct tcttcaagcg gtacgcgttc caagcctttg aaacccaaaa gctggttgat
    28441 gcggccttgg gcgatttgct gctcgtggtt catgacggca acggtttggc cgggtttgat
    28501 gcgtccgttc aagatacgac cgatgccgag gcggccggtg tagttgtcgt agtcgagttg
    28561 ggaaatttgc agttgcagcg tttcgtccgc gctgccgctc ggtgcaggcg tatattttaa
    28621 gatagtatcg aacagcggac gcatgtcgtt gctctcgtcg gtttcttcca atttggcgaa
    28681 accggacaga ccggaagcat aaacaatcgg gaaatccaac tgctcgtcgg tcgcgcccaa
    28741 gttgtcgaac agctcgaaag tttggtcgat gacccagctc ggacgggcgg acggtttgtc
    28801 gattttattg atgacgacaa tcggtttcag ccccaaagcc aaggcttttt tggtcacgaa
    28861 acgggtttgc ggcatcgggc cttcctgcgc gtccaccaac aagacgacgc agtccaccat
    28921 ccccaaaacg cgctctactt cgccgccgaa gtcggcgtgt cccggcgtgt cgacgatatt
    28981 gatgtggtag ccttcgtaat cgatggcggt gtttttggcg aggatggtga tgccgcgttc
    29041 tttttcgagg tcgttgctgt ccatcacgcg ctcgtcaacc tgctggttgg cgcggaatgt
    29101 gccggattgg cgcagcagtt ggtcgaccaa tgtggttttg ccgtggtcga cgtgggcgat
    29161 gatggcgatg ttgcggattt gtttcataat taatgttttt caaaaactgt aagagataac
    29221 tacgcattat accacgcttt accggacagt atttgggaaa tgaagatgcc gtctgaagcc
    29281 cttgcctttc agacggtata gtggattaac aaaaatcagg acaaggcgac gaagccgcag
    29341 acagtacaga tagtacggaa ccgattcact tggtgcttca gcaccttaga gaatcgttct
    29401 ctttgagcta aggcgaggca acgccgtact ggtttttgtt aatccactat atcttcttac
    29461 atactttcag ttatccggtt acgcagccac cttgccgccg gggcgatttc gccagccaac
    29521 agccctgccg caatgcctgc cgattctttt atgacatccg ccgccgcgcc gtgcagccac
    29581 gcgcccgcgc aggcggcttc aaaaaccggc acgccctgtg cgagcagact gccgatgatg
    29641 ccgcccaata cgtcgccact gcccgccgtt gccaatcccg cgttgccact ttcgttgaca
    29701 tagatttccg tatcggacgc ggcaaccaat gttttgtgcc cctttaaaac cacggttgcg
    29761 ccgaaaattg cccctatctt cctcactgcc gccgtccgat ccgcctgaac ctgtgcaacc
    29821 gtcgttccaa gcaggcgcgc ggcttcggcg gggtgtggcg ttaaaatcag gtttttacac
    29881 ccgcgtgcca gatttcgggt ttcggcatcg gttgataata tgttcagcgc gtccgcatcc
    29941 aaaaggacgg gcttgtccgt gtgttccgtc aaaattccgg caagcgttcc gaccgccgcc
    30001 ctacctgtac ccaatccaca accgacaacc caggcgttta tatcttgacg cttgctcaaa
    30061 ccgtctgccg tatccagcat aatctcggga aaaccggcaa taacggcaaa aggtagcgca
    30121 tcctgattga aacccgccca caccttgccg cagccgagat acatcgccgc cgatgccgcc
    30181 aatacgggcg caccgctcat ccctgccgat ccgccgacta cggcgagcgt gccgaaagtc
    30241 cctttgtgcg aatcttccgc acgggctttg aaaacatagg gaaaacgcaa tgccgtctga
    30301 agcatccggc ggcggctctc gcctgaaaga tgaaaaacgg gaaacattgc ccctccaatc
    30361 ggttaaaata aggctcgaaa acaaaccgat tctaccgccg gaaaccgatt atgaaaaaca
    30421 aaaccaaagt ctgggacctc cccacccgcc ttttccactg gctgcttgcc gcgtccctgc
    30481 cctttatgtg gtatagcgcg aaagccggcg gcgatatgct gcaatggcac acgcgcgtcg
    30541 ggctgtttat ccttttcctg ctcgtatttc gcctctgctg gagcatttgg ggcagcgata
    30601 ccgcccgttt ttcctgtttc gtccaaggct gggcaggcat acgcggctat ctgaaaaacg
    30661 gtattcccga acacatccag cccggacaca accccttggg cgcactgatg gtcgtcgcgc
    30721 ttttggccgc cgtctcattt caagtcggca cagggctttt tgccgccgat gaaaacacct
    30781 tcagcaccaa cggctacctc aaccatttgg tttccgagca tacgggcagc ctgatgcgga
    30841 aaatccacct caactttttc aagctgctcg ccgttttttc cgccatccat atcgccgccg
    30901 tcgccgcata ccgcctattc aaaaagaaaa acctcgtcct cccgatgata accggcttca
    30961 aatacatcga aggcaaaacc tcaatccgct ttgccggcaa agccgcgctt gccgccgcat
    31021 tatcggttgc cgcgcttgcc gcagccgcca tcctgctcct gtcctgaaac ccgacatcaa
    31081 tgccgtctga agcccttttc gcccttcgga cggcatcccc tctccgcgca aacggtctga
    31141 atgcccgaaa cggcttcagg ccgtttttcc gtccaaaacc gttttatagt ggattaacaa
    31201 aaatcaggac aaggagacga agccgcagac agtacggata gtacagaacc gatttactta
    31261 gtgcttcagc accttagaga atcgttctct ttgagctaag gcgaggcaac gccgtactgg
    31321 tttttgttaa tccactataa aacggaaaac cgctgcatcc gcatccgccc ccatatagct
    31381 ttcagcttct aacaaatacc tttttattat ttaaccgggg aaatcctttt gtctaaaatg
    31441 actcgcgttc cgacaaaaat accgcgacgc accatatcgg aaacaaggtg cgggcaaaca
    31501 gcaaaggagt tcgccagtga attatttccc cattttcgcc aacctcgccg gccgccccgt
    31561 attggtcgta ggcggcggtt cggtcgccgc gcgcaaaatc agcctgttgc tggatgccgg
    31621 cgcgcaagtc agggttgttg caaatcagtt gaatgctgaa ttaagtgcgc tggcggcaga
    31681 aaacaaaatc ctgtggcttg ccgaagaatt tcgtgccgaa cacatccgca cagttttcct
    31741 catcattgcg gcaagcagcg accaagccct caaccggcgc gttttccagc ttgccgaaag
    31801 ctgccaaaaa ccggtcaacg tggtggacga ccgcgattat tgcagtttta tttttccttc
    31861 gattatcaat cgcaatccga ttcaaattgc cgtttccagt tcgggcagcg cgcccgtcct
    31921 cgcccgtttg ctgcgtgaaa aactggaagc cctgctgcct cattctttgg gcgatatggc
    31981 ggaaatctcc ggaagatggc gcgatgcggt caaagccaaa ctgaaatccg ttaccgaacg
    32041 ccgccgtttt tgggaaaagc agttcaacgg ccgttttgcc gccctcgtta aaaatcaaca
    32101 aaccgcccaa gccgaacagg agttggcaaa gcagttggaa caaaattatc aaggcggttt
    32161 tgtgtctttg gtcggcgcgg gtcccggcga tgcggggctg ctgacgctca aagggctgca
    32221 ggaaatccag caggcagacg tggtgcttta cgacgcgctg gtttcagacg gcatactgtc
    32281 cctcgtccgc cgcgatgcgg aacggatttt tgtcggcaag cgcgcccggg gcgaccgcac
    32341 cccgcaagag gacaccaatg cgcttatggt caggctggcc cgcgaaggca ggcgcgtggt
    32401 gcgtttgaaa ggcggagacc ctttcgtgtt cggacgcggc ggcgaagaat tggaaacctt
    32461 ggcacgccat cagattccgt tttcggtcgt ccccggcatt accgccgccg tcggcgcgac
    32521 cgcctatgcc ggtatcccgc tcacgcaccg cgattatgcc caaagtgcgg ttttcgtaac
    32581 cggccaccgc aaggcggatg cgcccgacat cgaatggcag accctcgccc gcagccgcca
    32641 gaccttggtg atttatatgg gtgcgctcaa agccgccctg attgccgaac ggctgcaaca
    32701 gcacggacgc tcgcccgaca cgccggcagc cgtcatcagc caaggcacgc tgccggctca
    32761 aaaaaccgca accggcacgc tcgccaacct tgccgaactc gccgaaaccg cgccgaatcc
    32821 ggcattgatt gtcataggcg aagtggtggg gctgcacgaa aaactcgcct ggttcggtga
    32881 aaacggcgaa ggagaaaacc gggtcgggca ggcgtatccc gcattgggcg gactgaatgc
    32941 cggacaacgg gcggcataag gcgggaaaaa tggaaacgac actgttcaaa cccgccctgt
    33001 ggcagatacc gcatatcgga tccggcggcg aaaccgccct tgcggaaaaa acggaaaccc
    33061 tcaaacaacg cctgcaccgg attgtcggca gccaccggga tgcacggttt gccagcagcc
    33121 ttgcggcaga agatatggtg attaccgacc ttatcgccgg cgaaaacctc aatatcggca
    33181 tttttacctt ggatacgggg ctgctccaca cggaaaccct aaacctgctg gacaggctcg
    33241 gacgcgcata tccccatttg cggataaagc gtttccggcc ggttcgagaa gatgccgacc
    33301 gttatgtgga aagcaaaggc aggtttgcct tttacgacag cgttgaagcg cgccgcgaat
    33361 gctgccgcat ccgaaaaatc gaaccgctca accgcgccat tgcaggcgcg gacgcctggc
    33421 ttaccgggca acgccgcgaa caatccgcca cacgcacgga actgccgttt gccgaatacg
    33481 atgccggacg gggcatcggc aaatacaacc cgattttcga ctggtcggaa cacgacgtgt
    33541 gggcatacat cctcgccaac aatgtgcctt acaacgattt gtaccggcaa ggatttccca
    33601 gcataggctg cgacccctgc acccgccccg tcaaggcggg cgaagacatc cgcgccgggc
    33661 ggtggtggtg ggaaggcaga aacagcaaag aatgcgggct gcacaaataa ttcataaata
    33721 tcagacagaa gaaataagga atatggtatg acgaaaaccg aaccgaacaa cgcccaactc
    33781 gactggttgg aatccgaatc catccacatc atccgcgaag tggcggcgga atgcgaaaac
    33841 cccgccctgc tcttttccgg cggcaaagat tccgtggtcc tgctcgccct cgcctgcaaa
    33901 gccttccggc tgggcagcag gccggtaaaa ctgcccttcc cgctggtgca tatcgacacg
    33961 gggcacaact accccgaagt gattgccttc cgcgacgcgc aggcggcaaa actcaatgcc
    34021 cgcctgatag tggggcgcgt ggaagactcc atcgctaaag gcacggtggt cttacgcaaa
    34081 gaaaccgatt cgcgcaatgc ggcacaggcg gttaccttgt tggaaaccat cgaggcaaac
    34141 ggctttgacg cgctgatggg cggcgcgcgg cgcgacgaag aaaaagcccg cgccaaagaa
    34201 cggattttct cgttccgaga cgagttcgga caatgggatc cgaaggcgca acgccccgag
    34261 ctgtggtcgc tgtacaacac caggctgcac aaaggcgaaa atatgcgcgt cttcccgatt
    34321 tccaactgga cggaactcga catctggcaa tacatcgccc gcgaaaacct cgagctgccg
    34381 ccgatttatt acagccacag gcgcgaagtg gtcagacgca gggggctgct ggtccccgta
    34441 acgccgctca ccccgaaaat gccgtctgaa acctccgaaa tccttgatgt ccgcttccgc
    34501 accgtcggcg acatcagctg cacctgcccg gtagaaagca ccgcgtccac gccgacggag
    34561 attatcagag aaacagcggt tgccgacatt tccgaacgca gcgcgacccg gctggacgat
    34621 caggcaagcg aggcggcaat ggaaaaacgc aaaaaagaag gctatttcta aatttcaaaa
    34681 accctttaag cagatatgag gaagaaaata tgacggcaca acaccaaacc ccgctccgct
    34741 tcattaccgc cggcagcgtc gatgacggca aaagcaccct gatcggacga ctgctctacg
    34801 acagcaaagc cctgctgtcc gaccaaatca aaaccttgga atccggcaaa agcaaaggtt
    34861 tgaaagaaac catagacttc tccatcctga ccgacggact ggaagccgaa cgcgaacagg
    34921 gcattacgat cgatgtggca taccgctatt tttccacggc gaaacggaaa ttcatcatcg
    34981 ccgacacgcc ggggcacgaa caatacacgc gcaatatggt tacgggcgca agcaccgctt
    35041 cggcggcagt cgtgctggtt gacgcatccc aactggattt cggcgcgcag cccttgcagc
    35101 ttctgccgca gaccaaacgc cattccgcca tcctccgcca actcaactgc ccgcatattg
    35161 tggtggcggt caacaaaatg gacctgctgg attacagcga agacaaattc aacgccattg
    35221 tggaggcata ccgccgactt gccgaacagc tcggtttgaa agacgtgcat ttcgtaccgg
    35281 tgtccgcact cttgggcgac aacctcgttt accccggcgg caatatgccg tggtatcggg
    35341 gcgaaccgct gctggtcatc ttggaaaacc tgccgggggc ggacgaaacc ggtcgttccg
    35401 ctgccgattt ttatttcccc gtccaactgg tcgtccgcca agatgccgac aaggccgatg
    35461 atttccgagg ctatcagggg cgcatcgaac gcggttcggt cgccgtcggg caaaccgtcc
    35521 gcatcgaacc gaacgggctg accgccgaag tgtccgaaat catcacgccg aaaggggaag
    35581 tggcgcaggc ttttgccggc gaggcggcaa ccctccggct ggaccgcgac atcgatgttt
    35641 cgcgcggcga cctttttgtc gataaaaatt ccccgctcgc cccgcaaaaa catctggaag
    35701 ccacgctttg ctggtttgac gaacgtccgc tcaacaccgc gcgcaaatac ctgctcaagc
    35761 acggcacgca aaccgtgccg gcaaaagtcg gggaaattga aagcgtttta gatgtccgca
    35821 cgctggaaca agaggccggc gcggaatcct tgaagatgaa cgacatcgcc aaagtccgca
    35881 tcaaccttca aaaacccgtt acggcaacgc cttatgcgga aaacaccgcc gccggttcgt
    35941 ttatcctgat agacgaagcg acatacggca ctgttgcggc aggtatgatt ttatgagtga
    36001 acacgatatg cagaacacaa atccgccatt accgcctctg ccgcccgaaa tcacgcagct
    36061 cctgtcgggg ctggacgcgg cacaatgggc gtggctgtcc ggctacgctt gggcaaaagc
    36121 aggaaacggg gcatccgcag ggctgcccgc gcttcagacg gcattgccgg cggcagaacc
    36181 tttttccgta accgtccttt ccgcctcgca aaccggcaat gcgaaatccg ttgccgacaa
    36241 agcgtcggac agcctggaag ccgccggcat ccaagtcagg cgcgccgaac tgaaagacta
    36301 taaggcgaaa aacatcgccg acgaacgccg cctgctgctg gttacgtcca cccagggcga
    36361 aggcgaaccg ccggaagaag ccgtcgtgct gcacaaactg ctgaacggca aaaaagcccc
    36421 gaaattggac aaactccaat ttgccgtact gggtttgggc gacagttcct atccgaattt
    36481 ctgccgggcg ggcaaagatt tcgaccggcg ttttgaagaa ttgggcgcaa aacgcctgct
    36541 cgaacgcgtt gatgcggatt tggactttgc cgccgccgca gacggatgga caggccgcat
    36601 tgtcgcgcgc ttaaaagaag aagccgcaaa aaaccgggca acgcccgcgc cgcagacaac
    36661 gccccccgcc ggccttcaga cggcaccgga tggcaggtac tgcaaggcag acccctttcc
    36721 cgccgccctg ctggccaatc agaaaatcac cgcccgccaa tccgataaag acgtgcgcca
    36781 catcgaaatc gatttgagcg gttcggattt gcactacctc ccgggcgacg cgctcggcgt
    36841 ttggtttgac aacgatccgg cactggtcag ggaaatccta gacctgctcg gcatcgatcc
    36901 ggcaacggaa atacaggcgg gcggaaaaac cctgccggtt gcctccgcac tgttatccca
    36961 ttttgaactc acgcaaaaca cccccgcctt tgtcaaaggc tatgccccgt tcgccgatga
    37021 tgacgaactc gaccgtattg ctgccgacaa cgccgttttg caaggctttg tgcaaagcac
    37081 gccgattgcc gatgtgctgc accgcttccc ggcaaaactg acggcggaac aattcgccgg
    37141 cctgcttcgc ccgcttgcgc cgcgcctgta ttcgatttcc tcgtcgcagg cggaagtggg
    37201 ggacgaagtg cacctgaccg tcggcgcggt gcgttttgaa cacgaagggc gcgccagggt
    37261 gggcggcgca tcgggtttcc ttgccgaccg gctggaagag gacggcacgg tgcgcgtgtt
    37321 tgtggaacgc aacgacggct tcaggctgcc cgaagacagc cgcaagccga ttgtgatgat
    37381 cggctcgggc accggcgtcg caccgttccg cgctttcgtc caacaacgtg ccgcagaaaa
    37441 tgcggaaggc aaaaactggc tgattttcgg caatccgcat tttgccgccg acttcctcta
    37501 tcagaccgaa tggcagcagt ttgccaaaga cggcttcctg cacaggtacg atttcgcctg
    37561 gtcccgcgat caggaagaaa aaatctatgt gcaggacaaa atccgcgaac aggcggaagg
    37621 actttggcaa tggctgcagg aaggcgcgca tatctatgtg tgcggcgatg cggcaaaaat
    37681 ggcaaaagac gtggaagccg ccttgctgga tgtgattatc ggggcaggac atttggacga
    37741 agagggcgca gaagaatatt tggatatgct gcgcgaagaa aaacgctatc agcgtgatgt
    37801 ttattgatta aatataatcg ggaggaacac aaaatgaccg tacagaccaa gacaaaaggt
    37861 ttggcgtggc aagaaaaacc gctatccgac aacgaacgtc tgaaaaccga aagcaatttt
    37921 ttacgcggca cgattttgga cgatttgaaa gacccgctca cgggcggctt caaaggcgac
    37981 aacttccaac tcatccgctt ccacggtatg tatgagcagg acgaccgcga catccgcgcc
    38041 gaacgcgccg aggcaaaact cgagcccttg aaatttatgc ttttgcgctg ccggctgccg
    38101 ggcgggatca ttaaaccgtc ccaatggata gaactggaca aatttgcccg ggaaaacagt
    38161 cattaccgct ccatccggct gaccaaccgg caaaccttcc aatttcacgg cgtgccgaaa
    38221 gccaagttgc agacgatgca ccgcctcttg cacaaactgg gtttggattc catcgccacg
    38281 gcggcggata tgaaccgcaa cgtgctttgc acgtccaacc cgatcgagtc cgaactgcac
    38341 cggcaggctt acgaatacgc gaaaaagatt tccgaacact tgctgccgcg cacgcgcggt
    38401 tatctggatg tttgggtgga cggcaaaaaa gttcaaagtt ccgacgactt ccttcaagaa
    38461 gacgagccga ttttgggcaa aacctatctg ccgcgcaaat tcaaaaccgc agtcgtcatc
    38521 ccgcccttaa atgatgtgga ctgctacggc aacgacttgg attttgtcgc catttcagac
    38581 ggcaacggac agcttgccgg attcaatgtt ttggcgggcg gcgggctttc gatggaacac
    38641 ggcaacacca aaacctatcc gaacatttca ctggaactgg gtttcgtgcc tccggaacac
    38701 gcgctgaagg ccgccgaagc ggtggtaacc acacagcgcg acttcggcaa ccgcagcgac
    38761 cgcaaaaacg cccgcacccg ctacaccatt caaaatatgg gcttggacaa cttccgcgcc
    38821 gaagtcgaac gccgtatggg tatgccgttc gaacccatac gcccgttcaa atttaccggg
    38881 cgcggcgacc gcatcggctg ggtaaaaggc atagacggca actggcattt gacccttttc
    38941 atcgaaagcg gccgtttggt tgacgaaggc gggaaacagc ttctgaccgg cgtgttggaa
    39001 atcgccaaaa tccacaaagg cgatttccgc attaccgcca accaaaacct catcgtggca
    39061 aatgtcgccg aagcagacaa agcaaaaatc gaagaacttg cccgaacata cggattaatc
    39121 cgcaacgatg tcagcaaact gcgcgaaaat gcgatgtcgt gcgtttcctt cccgacctgc
    39181 ccgctggcaa tggcggaagc cgaacgcgtg ctgccggact tcatcgacga gctggataag
    39241 attatggcga aacacggcac gtcggacgac tacatcgtta cccgcattac cggctgcccc
    39301 aacggctgcg gacgggcaat gttggcggaa atcgggctgg tcggcaaagc cgtcgaacgc
    39361 tacaacctcc atatcggcgg cgaccgtgaa ggcgtacgca tcccccgcct ttataaagaa
    39421 aacatcaccc tgcccgaaat ccttgccgaa ttggacgacc tgatcggcaa atgggcggca
    39481 gaacgcaata tcggcgaagg tttcggcgat ttcgccatac ggacgggcat cgtcaaaccc
    39541 gtattaaatg cacccgttga tttttgggac gtatccaaag ccgtcgcgat tgcccgcgcc
    39601 tgaacccgtc cccgcattcc tgcggggatt ttcttttttg ccgcacgccg tttcaatccc
    39661 gcaaaattcc gatacttggt ataaaatacc ctcttttccc actctaaaaa accgtacccg
    39721 ataccgtctg aaacagccct gtctttcaga cggtataggc acaaggaaac acactatgcc
    39781 agaataccgc tccaaaacct ctacccacgg ccgcaatatg gcaggcgcac gcgcattgtg
    39841 gcgtgcgacc ggcgtgatgg aaaccgactt cggcaagccc atcatcgccg ttgccaactc
    39901 cttcacccaa ttcgtgcccg gccatgtcca cctgcacaat atgggccagc tggttgcccg
    39961 cgaaatcgaa aaagccggcg caatcgccaa agaattcaac accatcgcca tcgacgacgg
    40021 catcgctatg ggacacagcg gcatgctgta ctccctgccc agccgcgatt tgattgccga
    40081 ctctatcgaa tatatggtca acgcccactg cgccgacgcg ctggtgtgca tttccaactg
    40141 cgacaaaatc accccgggca tgctgattgc cgcgatgcgc ctgaacatcc ccaccatctt
    40201 cgtctccggc ggcccgatgg aagcaggtaa agttatcggc gtggcaaaca tccaacccga
    40261 acgccgtttg gacttgattg acgcgatgat cgaatcggca gacgacaatg tcagcaaccg
    40321 gcaagtcgaa gaagtcgaac aaaacgcctg cccgacctgc ggctcgtgtt cgggtatgtt
    40381 tacggcaaac tcgatgaact gcctgaccga agcactcggc ctttccctgc ccggcaacgg
    40441 ttcgtatttg gcgacccacg ccggccgcaa agaattgttc ctcgaagcag gccgtatgat
    40501 tgtcgaaatc accaaacgct attacgagca agacgacgaa accgtactgc cgcgcagcat
    40561 cgccaccaaa aaagcgtttg aaaacgctat gacgatggac attgcgatgg gcggctctac
    40621 caacaccatc ctgcatttgc tcgccgtcgc caacgaagcg ggcgtggatt tcaaaatggc
    40681 cgacatcgac cgcttaagcc gcgtcgtgcc ctgcatctgc aaaaccgcgc ccaacaacca
    40741 cgactactat atggaagacg tgcaccgagc cggcggcatc ttcgccatcc tgaaagaatt
    40801 ggacaaagcg ggcaaactgc acaccgacgt gcacactatc cacgcaccga cgctgaaaga
    40861 cgcgattgaa aaatgggacg taaccaaccc tgaaaacacc cgcgccatcg aacgcttcaa
    40921 agccgcgccc ggcggcgtac gtaccaccca agcgttctcg caaaaccgta tgtggaaaac
    40981 cctcgacctc gaccgcgaaa aaggctgtat ccgcgacgtg gcacacgcct actcgcaaga
    41041 cggcggtttg gcggtcttgt tcggcaacat cgccgaacgc ggctgcgtgg taaaaaccgc
    41101 aggcgtggat gagagcatcc tcaaattcac cggccgcgcc cgcgtgtttg aaagccaaga
    41161 agacgcagta gaaggcattt tgggcaacca aatcgtcgcc ggcgacatcg tcatcatccg
    41221 ctacgaaggc ccgaaaggcg gcccgggcat gcaggaaatg ctgtatccga cttcctacct
    41281 gaaatctaaa ggcctcggca aagcctgcgc tctcttaacc gacggacgct tctccggcgg
    41341 cacatcaggt ttgtccatcg gacacgcctc gcccgaagcg gcggaaggtg gcgcgatcgg
    41401 tttggtacac gaaggcgata ccgtcgaaat cgacatcccc aaccgcagca tccaccttgc
    41461 catttccgat gaagagcttg ccgcacgccg tgccgaaatg gaagcgcgcg gcagcaaagc
    41521 atggaagcct aaaaaccgcg accgctacgt ctccgccgca ttaagagctt acggcgcgat
    41581 ggcgacttcc gccgacaaag gcgcggtgcg cgacgtagcg caaatcgaaa gataagcccc
    41641 aaaacgtgcg gcaaatgctg tctcgtctga acagtaaagg tctgatgatg gacaaattta
    41701 tctgggcgat ggcggtattt tccgcaattt tggcctcgtt atcggcagcg gctttagcgc
    41761 gatcgagtat gtaaaagagc tgccgctcgc cgccgttgcc gtgatgcctt gcgcgccgaa
    41821 gtcgttgctg tggttgttgg cgaagctgag gtagtcgaat cccgcgtcgg caaggtattg
    41881 cccgtatgcg gagggcgttc ggaatgcata gcatattttg ggggtttgca cattttttcg
    41941 gcgtaccgcc ttcgtcaaac agcgtgcctt cgaggttgcc gacggtaatg tccgcgtctt
    42001 gcaaggcaga ttcgacgttt ttcagaatat tggtatcggg caggtaatcg accggataat
    42061 tgctgccgag cataatgtcg cccacgccga taatggaaac ggtatcggct gtcttttcgt
    42121 caggcgcatc ctgtccgtcc acatcgccgg tatttgaaac gggcgcgggg tttgaaccgt
    42181 caatcggcag cacggggatt ccgttccggc aggatgcgcc gagggcttga ccccggcggt
    42241 ttgcggtgcg gaaacggcat aaagggcgaa ccttgacgaa ttgaacatca aatattcggg
    42301 catcgaaaac aggtttgaaa ccgccatcct gaagaaaaac ggcgtaagat ccggcttcgt
    42361 ctccttcgcc cccaaccttg ccgccgtcaa actgaacgat tatgccaaag ttagaaaacg
    42421 gattaccaaa accaaacaga aagccgacat cgtcattgtg atgttccacg gcggcgcgga
    42481 agggaaacag gcggaacacc tgccgttcga taccgaaatc ttctatgggg aaaacagggg
    42541 caacgtcgtc gagtttgcac ggcttgccgt cgattccggc gcggatgtcg tattcgggca
    42601 ggggccgcac gttacacgcg ccgtcgaact ttaccgcgac cgcttcatct cttacagcgg
    42661 cggcaacttt gccacctacg gcgcaatcaa cacctccggc atcagcggca tcgcccccat
    42721 tttcaaaatc atcaccgaca aacagggtaa atttatttcc ggaaccatca tccctattac
    42781 ccaagccgac gataaaatcc ccaaaatcga ccccgagaaa accgttatcg ggcggattat
    42841 ttatctgaac cgcagcgatt tccccaacgg gaacgggctg gatgtctcgc ccgacggcaa
    42901 catcacgcgc cggtaaaaag atattggaaa taccgtccga acaaatgccg tctgaaagcc
    42961 tttttccggg tttcagacgg catttttgca atcgtcaata atccacttta atcccagagg
    43021 ctttttccac ttcggcgacc tcgccttcca gctcaaactg taagggatac aggttaagct
    43081 tttccatcag gattctgtcg ccgtcctcat caggattgcc tgtcgtcaac agcttgtcgc
    43141 cgtaaaaaat cgaattcgcg cccgccataa agcacatcgc ctgcatcgct tccggcatat
    43201 tgctgcgccc tgccgacagc cggacataac tttgcggcat cgtaatccgc gccacggcga
    43261 tggtgcggac aaattccgtc cagtccaaat cttcggcatc gtcaagcggc gtgccttcca
    43321 ctttgaccaa ccggttaatc ggcacgcttt cgggctgcgg gtcgaggttg gcgaggctgg
    43381 caatcagccc ggcgcgttcg gcacgagttt cgttcatccc gacgatgccg ccgcagcaga
    43441 ctttcaaacc ggcgttgcgg actttgccca aggtgtccat acggtcttca tgccggcgtg
    43501 tatggatgat gtcgttgtag cggtcggggt cggtgtcgag gttgtggttg taataatcca
    43561 agcccgcctc tttcaagtct tccgccatac cttcttcgag cataccgaac gtgccgcagg
    43621 tttccatacc caagcccttg acggctttga tgattgcgga aaccgtctcc acgtctttgg
    43681 gtttggggcc gcgccatgcc gcgcccatac aaaaccggct tgcgccgcgc gatttggcga
    43741 ttttggcttt ttcgacgatt tcatccacat ccatcatctg ctctttgccc agattggtat
    43801 tgtggtgcgc cgattgcgga cagtaggcgc aatcttcggg acaaccgccg gttttgatgg
    43861 acaacagcgt ggaaagctgg atttcgcgcg ggttgaaatt ttggcggtgg acttctgccg
    43921 cctgatggac aagttctaaa aacggcagtc cgaaaagtgc ctcgacatcg cattttttcc
    43981 aatagcgcgc ggtgggatga ggcttgcact cggtcttacg gcgcaaggcg acgggggata
    44041 cggtcataat gtgttctttc gtatttacag cggcgcaatg acgctgacag gcaaaccgcc
    44101 ggcgcaatga cggcggttgc gggtaatcaa atggctgaaa acagcggcgg caagtgtaac
    44161 gcgtttgaaa atacggggca aacggttttt ccgcctttgt gccgcgcagc cgggcgatgc
    44221 cgtctgaagg gctttcagac ggcatcgccc gggctatctc aaagcgtcca gcagtttggt
    44281 gtgtattccg ccgaaaccgc cgttgctcat caccaaaata tggtcgccgg ttcgggcgtt
    44341 tttcacaatt tcggcaacga aggcatcgaa atccttgccg acgtgcagcc tgccgcccaa
    44401 aggtgcgagg gcttcggcga cgttccagtc cacaccgccg gcgtagcaga acacttggtc
    44461 ggcttctttg aggctttcgg gcagggcgga cttcatcgtg cccagcttca tcgtgttgga
    44521 acgcggctcg aggacggcga ggatgcgggc gtttccgacg cgctgacgca gaccttctat
    44581 ggtggtttcg atggcggtcg gatggtgggc gaagtcgtcg taaacagtga tgccgtttac
    44641 cgtgcctttg atttccatgc ggcgtttgac gtttttaaac gtgctcaagg cttcgcaggc
    44701 cgtctgaatg tcgactccgg catgacgcgc ggcggcgatg acggcgagcg cgttcatgcg
    44761 gttgtgtccg cccatcaaac tccaagcgac gtgtccggct tttttgccgt caagcaacac
    44821 gtcgaacgag ccatcggcat tggcttcgcc ggcctgccag ccgtgttccg tgccgaattt
    44881 ttccaccggc gtccagcagc ctttgtccaa agtgtcttgc aggctttgct gccgtccgtt
    44941 gcagacgatg aggccttcag acggcacggt acgcacgagg tggtggaact gggtctgtat
    45001 cgcgcccaaa tcggcgaaga tgtcggcgtg gtcgaattcc agattgttca acacggcggt
    45061 acgcggacgg taatgcacga atttggagcg tttgtcgaaa aacgcggtgt cgtattcgtc
    45121 ggcttcaatg acgaaaaacg gcgattggct gttcgggtct tggcgcggcg tttgcggcag
    45181 gcgggcggaa acgctgaagt tttccggtac gccgccgata aggaagcccg gtgcgagtcc
    45241 ggcatattcc aaaacccatg cgagcatgga cgcggtggtc gttttaccgt gcgtccccgc
    45301 cacaccgagt acccaatgat ggtgcagcac gttttcagcc agccattgcg gaccggaaat
    45361 ataaggcagc ccgcggttca aaatcgcctc aaccacatcc atcccgcgct tggcgacatt
    45421 gccgatgacg taaacgtcgg ctttaaattc ctccaactgc gccgcgtcga agccttcgtg
    45481 tacgcctatg cccaaggctt cgagctgggt gctcatcggc ggatacatct tcgcatcgca
    45541 accgctgact tcaaaccctg cttctttggc aatggcggca atcccaccca taaacgtgcc
    45601 gccgataccg ataatgtgga tgtgtttcat cagagttccc taatcgttca aattctatta
    45661 ttcaacgccg tcatggctga ttcggtttca atcaatgcgt ctttgcaccg gtattcgaag
    45721 catccttatg cgtattctct ctcggatttt ggacgtgttc gtccctcacc gtgtccgaat
    45781 aatcgccgac ccctgcgtct tccgccctgt cttttactgc cggtctaacc gaactgtaca
    45841 cccgatcccg ccccaaaccc gcgttggtac aatgttttcc cggagtttgg ctgtaactgg
    45901 ttttaccatt atggcggcac gcgtaaacat ttgccgaaaa tacggtctga accgctgtaa
    45961 caagcaacat acttccaatt attttagata cgcgcatatc aacctccttt atcgttcttc
    46021 ttcaaaaaag ggaatgtatt tcaatagcac ttcatctaat tctttcattc cctgctcata
    46081 attgaacggg tattcccaac catcccctac tttgatttca gaaatatctt taaacttccg
    46141 cttatgggcg gacactttgt ccatatcgtc atcaatttcc tgtttgccga acggtatttt
    46201 ccgcttttcc aaaacctcgt cataataaca agataaatta agcaatagcc cccaagcact
    46261 atttgcaatt acgactaatc tatgtttttc tctggttaaa taatctgcac cgctatcata
    46321 gattgccctt gtcgggtcat aaaaaaattc gcaatttttc atagcgtccc cacactttaa
    46381 ccgattcgca attttatatc cgcgattttg caagctgccc ataaaaacca attgcctgcc
    46441 aagccggcac tacttgttaa agatgcccga taatgccgca actcttccgt atttaccgac
    46501 aaacgcccta tttgcttaga gctgttttgt ttcccgaaaa actttaatcc attgatttaa
    46561 caaatttttt accaaattat ttatttgttc ggattccatt aacccaattt tgagcaatat
    46621 ctgtcaaaac catttccacc acatccaaaa caaaaccgcc gccgccacgg tattggacaa
    46681 taagatgagt ttccactgcc cgtccttccg caatgccgcc ttcttgggct tcaaagcacc
    46741 cgcataaaaa aacggcgaca gcagcataga agcgatacat aagaaaatca atacgccgta
    46801 atagattttt tcagacggca tttcttttcc tcaatacgcc gaccgctgcc gcatctgttc
    46861 aaacaggcag attgtcgccg ccatcgcgac atttaaagac tcggttgcat cgtgcatcgg
    46921 aatcctgaca cacttgtccg ccctgtccaa aaccgcttta ccgacccccg cgccttcgtt
    46981 gccaaacacc caggctgtcg gttcgcgcaa atcttcgtta tacagaaccg cctgtttttc
    47041 ctcacgcaac gcggtggcaa acacacggcc tttatagctc gccaaccata tttccaaatc
    47101 cgcctgcgga taaatctccg acaagaaatg cgcgcccatt ccggctcgca gcactttggg
    47161 cgaccacgcg tccgcacaac ctttgcccaa aacgaccgcg ccgattcctg ccgccgccgc
    47221 gctccgcaac accgtgccga cattgcccgg atcttgcacg ccgtccaaaa ccacacagtc
    47281 gccgccggcc ggcaaagcac ccgcatccgg aatatcaatc agcgcaagca catcatccgc
    47341 gcaagtcagg ctgctgattt tcttcaatat gccgtctgaa acggaaaaaa ccccgtcttc
    47401 cggcaaaacc gccgtcaatt tacggacttc ttcagacggc attttcgctt cgggaatata
    47461 taccccgact ggccttccgc cggattgcag gaaaacctga agcaggtgca cgccctccaa
    47521 aacggtttgg gcgtattgcc gtctgaactt cccttgagac aacaggcggt gcaggtgtct
    47581 gatgtgttca ttgtttgccg aactgatatg cttcacttta ccgccttttc aagaatatat
    47641 aataggtaac ggcgattccg attccatcaa aataaaacgg gaaaatccgg atggacgggc
    47701 aaaatgccgt ctgaaaccga gatttgaaaa tccgtaccgt aatttgccaa cgccagcgga
    47761 cggttgtcct tgtcggcgac gaccggccaa aacgggcgca gcgcgggcaa aacccgcttt
    47821 tcctgaagga ttttcttcac atccttatgg ataccgccga cagccagcct gtccgatgcc
    47881 gcaaccgtcc gcaagattcc gtcctgctcc aaaagatgcc cgggaagtcc gaacggatga
    47941 cgttttaaaa caaatccctg ccccgccaat atttccctta aatttccgct tatctgcctg
    48001 tctttcaaaa accgcagttt atccgttttt tccggtcgga acacaaacag cctgcctgca
    48061 taatgatgca attcaaagcc ttgcacgttc caacgtccgg tttttgcctc cgtcaaaacc
    48121 cgggcaatgt cggcaagggc attctgattc ggcacgggaa tgccgttttc cttcaaaaaa
    48181 tgccgcaaaa tatgggtttt ccggcgcggg gaaaacgtca gccaccgcgc cgtatcgaaa
    48241 taaccggctc cgcaaaccca acggcagtcc tgaccgatga tttcgtccaa caaagccaaa
    48301 tcttcctgca aagcgcggac attgttcagc acatgccgcc cgaaatgggg aatctgcgcc
    48361 gaaagttcgg gcaaaatacg gtgccggaag cggtttcgca aataagccgt atcgctattg
    48421 ctttcatcct cgatattcgg caaaccgtgt ttttgggcat aatcccatat atcttggcgc
    48481 gaaaaaggca gcaagggccg ccagatgatg cctttttccc caaaagggcg gacggcgggc
    48541 atagccgcca aagcgcgcaa accgccgccg cgtgaaactg caagcataaa ggtttcgatt
    48601 tgatcgtccc tgtggtgcgc caacgccaaa acgtcaaagc ctttttcggc aaacgcggca
    48661 taacgctttt gccgcgccgc cgcctcgatg cccaaaccgt ttttttccac gcagacctta
    48721 accgtttcca gccccacccc gagcatatcg caatagtttt ggcagaaatc tgcccaatcg
    48781 tcggcacggg gactcaagcc gtgatggata tgcaatgcat ccggaataaa accgcccttt
    48841 tttccggcgc ggacaagcag atgcagcaaa acgacggaat ccaagccgcc gcttaatgcc
    48901 accgctgttt tttttccatt cagaccttga ggaaaacaat ccttcaagca ttgctcaaac
    48961 gcatctaaag tcagcacggt tccgtcccga taaagaaaaa gcaagcagtt tcagacaaac
    49021 tgcttgctgc ttgttctacc tgaattattg ttccgaaaat ttgccgtaag ccataatgcg
    49081 gtcgaaacgg cgcgaaagca aatcggcaag cgggatgctt tgcgcttcgt gcagttgttt
    49141 ttccaaaacg gcttttacgt ttttcatggt ttgcccgaaa tcccgatgcg cgccgcccaa
    49201 tggttctttg atgacggtat cgaccaagtc cagcttttgc aggcggtcag cagtaatgcc
    49261 caaagcctga gccgcatccg ccgccttttc ggcggttttc cacaaaatcg aagcgcagcc
    49321 ttcgggggag ataacagaat aggtcgaata ttgcagcata ttgacgtaat cgcctacggc
    49381 gaccgccaac gcaccgcccg agcccccttc gccgatgacg gtacacaaaa tggggacgtg
    49441 caggcgcgtc agttcgtaca ggtttttgcc gatggcttcc gactgcccgc gttcttccgc
    49501 gccgatgccg ggatacgcgc ccggcgtatc gataaaggtc atgacgggca agccgaattt
    49561 ttctgccgtc ttcatcaggc gcagggcttt gcggtagcct tcaggacggg gcataccgaa
    49621 gttgcggcgg attttttctt tggtgtcgcg ccctttctga tgcccgacga ccatcacgct
    49681 ttgtccgttg aaacgtgcca atccgccgac aatcgcatga tcgtcggcaa aatggcggtc
    49741 gccgtgcatc tcttcaaaat cagtaaacag cgcatcaatg taatccaaag tatagggacg
    49801 ctgcggatgc cgggaaacct gtgaaacctg ggcgggcgta agtttgctga aaatagactt
    49861 ggtcaaatcg ttgctttttt tctgcaaacg gtgtatttcg tccgaaatat cgacggcaga
    49921 ctcgtcttgg acgaaacgca gctcatcgat tttgttggtc agttcggcta tgggttgttc
    49981 aaaatccaaa aaaacaggtt tcatagaatg aagctctcat caacagattc tgccgctatc
    50041 atacgctatt tgtgcatatt ccgcagttaa tttattgtcc aaaacggctt ttccacaccc
    50101 tctttttgtg ttcggacagc atggaaaaca gtggattagt gcatatatac tagttatagg
    50161 cggcgtatct tatgctaaac tcgaaacaaa ttcaattatt tcaaacaaaa tcgtatgaaa
    50221 gacaagcacg attcttccgc catgcggctg gacaaatggc tttgggcggc acgttttttc
    50281 aagacccgtt cccttgcgca aaagcacatc gaactgggta gggttcaagt aaacggctcg
    50341 aaggtcaaaa acagtaaaac catagacatc ggcgatatta tcgacctgac gctcaattcc
    50401 cttccctata aaatcaaggt taaaggtttg aaccaccaac gccgcccggc atccgaggcg
    50461 cggcttctgt atgaagaaga cgcaaaaacg gcagcattga gggaagagcg caaacagctc
    50521 gaccaattca gccgcatcac ttccgcccat cccgacggca gaccgaccaa gcgcgaccgc
    50581 cgccaactgg ataggctgaa aaaaggagac tggtaaaacc gttcccactg caaacagaag
    50641 cacgaaccgc gtaccgccgt atttcagacg gcattcccga aacttattct gcctcgtcta
    50701 tccatgcctg ctgcacggct tcgaggatgc gttcgccgca acgggcggga tcgtcatcaa
    50761 attcgggcaa tgccataatc aggtcgcgca gttgggtaaa gcgcacggtt ctgggatcga
    50821 tggtttcgcc gtgcaggtca tagagttctt cggcgatacg ccgggtgtcg gtccatttca
    50881 tatgaatttc cttttatcgt ccggcattga agccgtgtag aatatgtttc ggcaaaactg
    50941 ccgcaccttg aggatggctg aatgtttcaa ctcgccggac aacacatcaa gcaccagcct
    51001 atgtgtcgtc ctgatctggg aggagttgtc cctcccaaac aaatctgatt ctaccgcccc
    51061 gaagagcggg gtttcaaccg acaaggaaga ttgatgaaca atatgtttgc cgcaaaattg
    51121 tccaaactgg tttataccgc ttccgaccat cctgattccc tgtcggagat ggaggagttt
    51181 gaccgcctga ttctgctgat acgcaaactg tatcaaatat tggacgggca acatatcctc
    51241 tccagagtaa cggtttgcct tcaccaccaa aaccggcgcg gtctgattgc cttggataaa
    51301 gcggctgccg gttgcgatgc ggcaatgttg cgcgcccaac gttggcgcat ctacacgcag
    51361 attgcggaac gccttgccgg gtcgggcggc ggcttcacgg ttacggcaga aagcgtgtcc
    51421 gccgcctgtc cggagcttga aggacgctat ctcgagcttg tccgccgcac cgccacctct
    51481 ttcggtttcg tgcaagtaca ggaatatccg gcaggcgggc aggaagaaac acaagccgtc
    51541 ccggatgccg catcggatca ttccgtcaga aaaccaatca aaatcaatat agaagcgaaa
    51601 gtggaacgct atccggcata ccgcccgacc gccgccctga aagtaacccg ccggcggttt
    51661 ttccggctgc aaaaccgata aatacccgtg catcgcgccc gagtaatccg cctcggatgc
    51721 cgcaccaatc cgttccgtca gaaaaccgat aaaaatcaat atatagtgaa ttaaatttaa
    51781 accggtacag cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc tcgccgcctt
    51841 gtcctgattt ttgttaattc gctatagaat cgaaagttcc gcctgtacga acgcctgttt
    51901 tcgggcgatt gcgccttatc cgcgcaacaa tgccgtctga agcgtgccgg cttcagacgg
    51961 cctgttttca gtgttcttcg cgcgcgtggt tgatggtgta tttggggatt tccacaatca
    52021 aatcttcgcc ggcgacaacc gcctgacaac tcaggcgtga atcggcttcc aaaccccaag
    52081 cctggtcgag caggtcttct tccaattcgg tcggttcttc caaactgtcg aaacctttgc
    52141 ggatgatgac gtggcaggtg gtgcaggcgc aggatttttc gcaggcgtga tcgacttcga
    52201 tatcatggtc gagcagcaca tcaaggacgg ttttgccttc gggcgcgtct tcgataaccg
    52261 cgccttcggg gcataatgtc gtgtgtggaa gtacggtgat tttgggcatt tttattgtct
    52321 ttctgtaaaa agtgtttgtg ttcgttttcg gaaaactgtc cgcctaatgt cgcggttcga
    52381 gcaggtcggc aacattttgc cagcccgcca ggcgggcaaa atcgagggcg gtatagcctt
    52441 ccgatgtctt tgaggcagga tttgcgccgt attcaagcat ggcttgaacc ataagtgcat
    52501 ttcctgcgat ggcttcatga tgaagcggcg tatagccgtc ttcgtcctgc tcatgcagaa
    52561 aatccggatt ttgggacaac tgttgccgaa aatcgtcaat acagttccgg cacatcggat
    52621 gctcttccag attttcggga tgttcttttt cttcatacac cagcaatacc tgcccgggat
    52681 cgccgaaatc gattccccat gcggcatcgt gttcggtacg ctcctcttcc gtcatctgtc
    52741 cacgcattgc ctgtatggta aagccgccgt agggaatgcc gttgcacacg aacatccagt
    52801 cgctgatgtc gtcaaccgga acgcaaacgc tttcgccttg ttcgacattg gtcagttcgc
    52861 cgggttcatt gttcagcacg ccataaatgt aaagaccgtc aaaataaata tcgtcgatcc
    52921 acatatgttc gcaaatttcg ccgtcttcgc cgtcttggaa aaaaggggct ttgaccatgg
    52981 caaaatctaa ggcgggaatg atgcggcggc gttcccaaaa aagctcgcgc caaaaatatt
    53041 tgaatgtttt acgggcgcgt tcgccggcac ggtttaccgg tttgtctgcc tgttctacat
    53101 aataaatgac ggaatcgccc atcatatctg ctcctcaacg tgtacggtat ctgtttgcac
    53161 cttactgcgg ctttctgcct tcggcatccg attcggattt gaaaagttcc aaatattcgg
    53221 aataggcttt ttccaaatcc gcccgcgaaa aacaggtgta aatatggatg ccgtctgcaa
    53281 tttcgtcggt cagcctcgtg ccgcttttgc cgaaatatgc ttctttaaaa aggcggattt
    53341 tcgatttttt cggcttgtag gatgccgcgc ccgccaaaag ccggggcagc gcgtaagggt
    53401 attcccggtg tccgcgccgt accaaatcaa gcagttgttt gcggccggac ccggcattcc
    53461 cttcctgcct gcgttcgtgg acgctttttt caaattccga ttccgccgtt tcgtttttcg
    53521 gaaacacgat ttcgccgcga agcgataaaa acaaacgttt tacaaggttt tcgtccacgg
    53581 cgaacaattc ggtatcgcct atcctgcttt tgctgaaaat ttcatggagc aggttttctt
    53641 tttctttgta attgtcggtt ttgacggcga ggatgcgttc caatccggca acgttcgcat
    53701 agccgttgtt ttccaaatgg cgcattctgc cttcaaaatt gcttacctcc gaaatgccga
    53761 ttttatatag tgaattaaca aaaatcagga caaggcggcg agccgcagac agtacaaata
    53821 gtacggaacc gattcacttg gtgcttcagc accttaggga atcgttctct ttgagctaag
    53881 gcgagccaac gctgtaccgg tttaaattta attcactata cacgcccttg atgacggttt
    53941 tcatcgaata gacaatgcct gacttttcca tatcgatgtt tttcaagtgt tttcgagcct
    54001 tcagacggca tcaggttata tctatgccgt ctgaaaccgt ttaagtatca aatattatcg
    54061 acactctggc ctgtcaacgc gcgttggatg ttgcggttca tgcgtttggc ggcgaaattg
    54121 tcggtaatac tgccgagttt ggcggcggcg gcacggatgt cttcggcttt tccgtctttc
    54181 agacggcctt gcaaatcggc gatgccttgc cgaatctgtt gcaattcttc ggcatccagc
    54241 aaatcgctgt ccaactcgag ggcggcgttg acggcatcgg tcaggctttc ggcttcgact
    54301 acggcttcgg cacgggcgcg tgccgccata tcttcggcgg cattgctcat gctgtctttg
    54361 agcatttggg tgatggtgtc gtcgtccaag ccgtaggagg gtttgacttc gatttgcgcg
    54421 tgtacgccgg tgctttgctc ttgggcggaa acggacagca gcccgtccgc atcgatttgg
    54481 aaggttacgc ggatacgcgc cgcacccgcc gccataggcg gaatgccgcg cagggtgaat
    54541 ttggcaaggc tgcggcagtc ggcaaccagt tcgcgttcgc cttgtacgac gtgtatcgtc
    54601 atcgcggtct gaccgtcttt gaaggtggta aagtcctgcg cgcgcgcggt ggggatggtg
    54661 gaattgcgcg ggatgatttt ttccgccaag ccgccgtagg tttccaaacc gagcgacaaa
    54721 ggtgtaacgt ccagcagcag ccattcgccg tcggttttgt tgcctgcgag gacgtttgcc
    54781 tgtatggcgg cgccgagcgc gacgacttcg tcgggattga ggttgtccag cggggtttgt
    54841 ccgaagaagg tggcgactgc ctgctggacg tgcagcatac gggtcgaacc gccgaccata
    54901 attacgcctt tgacttcgtt tttaccgaca ccggcatctt tcaacgcctg tgtgaccggt
    54961 tcgagcgttt tcatcaccaa atgctgcgtc aggttgtgga actcggcgcg actgatgctt
    55021 gtgtcgattg ccatgccgtc tgaaagcgtc gcctgaatgc gcgcttcggt ttgcgtggtt
    55081 aattgttctt tggcggcgcg gacgagcgaa agcaggagtt ggctgtcttg ttcgttgagt
    55141 tgggagagtc cgttttgttc gagcaggcgg cagaacaggc ggtggtcgaa atcgtcgccg
    55201 cccaacgcgc tgttgccgcc ggtggctttg acttcaaaca gtcctttggt cagttgcaat
    55261 acggatacgt cgaatgtgcc gccccctaag tcgtacacga caaacgtgcc ttccgaggcg
    55321 ttgtccagcc cgtaggcgat tgcggcggcg gtgggttcgt tgagcaggcg caatacgttc
    55381 aaacccgcca gacgcgcggc atctttggtg gcctggcgtt gggcatcgtc gaaataggcg
    55441 gggacggtaa tcaccacgcc gaccaaatcg ccgcccaagg tttcttcggc gcgcgattta
    55501 agggttttga ggatttccgc cgacacttcg acaggcgttt tcaccccctg ccgcgtatgc
    55561 agttcgataa cgcgttgatt gtcaccgaaa cggtaaggca ggtaatgcgt attttgatgc
    55621 agatcggcaa gcgtccgccc gataaggcgt ttggcggagc tgacggtgtt cagcgggtcg
    55681 gttttttggg cggacagggc ggttttgccg acttcaatgc cgccgttttc cagatagcgg
    55741 acgacggaag gcagggtaat gcgcccttcg gcatcgggca gacaggcggc actgccgctg
    55801 cggacggtgg cgaccaagct gttggtcgta cccaaatcga tgcctgccgc caaacggtgt
    55861 cggtgcgggg cggcggacat acccggttct gaaatctgca aaagagccat aaattctgcc
    55921 tgtcggtgcg gtgtgtaaag gaaggaagtc ggcattttaa cagaattcgc gccgatagtt
    55981 gagcaaaccg ctaaggattg ggtcgtgccg ccgaaattgc cctctaccgc caaatgccga
    56041 taaaatccgc ctttcccgcc catcattagg aatataaatc gtgaaaggac tcgactattg
    56101 ccgtcaaaaa gcagaagaaa gccgctccag ttttttatcg ggcttccgtt tcctgactca
    56161 ggaaaaacgg gatgcggtaa cgattttata tgccttttgc cgcgaattgg acgatgtggt
    56221 tgacgaatgt tccaaccccg atgttgcaca ggcaacattg aactggtggc gcggcgattt
    56281 ggacaaggta ttcggcggcg cgatgccgga acaccccgtc aatcaggcct tgcggcaggt
    56341 taaggaaacc ttcaagctgc cgaaatatga actggaagcc ttaatcgacg ggatgcagat
    56401 ggatttggtt caggcgcgtt acggcagttt tgaggaattg aaactgtatt gccaccgcgt
    56461 cgcaggcgta gtcggctacc tgattgcgcg gattttgggg ttttcagacg gcaaaacgct
    56521 gcaatacgcc gacaagatgg gacttgcgct gcaactgacc aacatcatcc gcgatgtcgg
    56581 cgaagacgcg cgcaggggac ggatttacct gccgatggag gaaatgcagc ggtttgatgt
    56641 ccccgcaagc gtgattttgc aatgcagccc gacgggcaat tttgccgaat tgatggcgtt
    56701 ccaaatcaaa cgcgcccgtg aaacctaccg cgaagccgta tcgctgctgc cggctgccga
    56761 taaaaaagcc caaaaagtcg gactggttat ggcggcggtt tattacgagc tattgaacga
    56821 aatcgaccgg gacggcgcgc aaaacgtcct caaatacaaa atcgccctcc cttcgccgcg
    56881 caaaaaacgc attgccctga aaacctggtt attcggattc aaaccgcgcc ccggcacgcc
    56941 ggaacgggca taaggcgcat accgcccgcc cttcaggaaa acccaacccc cgccgccccg
    57001 aaaaacgggg tttcaaccat tggaggaata aatgatgaac acgccgcatc cgcgcccgaa
    57061 aatcgccgtc atcggcgcag gctgggcagg actgtccgcc gccgtcacct tggcgcggca
    57121 cgccgacgtt accctgtttg aagccggccg gcaggcgggc ggcagggcgc gcgcactggc
    57181 cggaaatacc gacggtttcg gttttttgga caacgggcag cacattttgc tcggcgcata
    57241 ccggggcgtg ttgcgcctga tgaaaaccat cggttcagac ccccgtgccg cctttttgcg
    57301 cgtaccgctg cactggcata tgcacggcgg tttgcagttc cgcaccctcc ccctgcccgc
    57361 gccgctgcat attttgggcg gcgtgctgct tgcccggcgc gtaccgtccg cattcaaagc
    57421 caaactgctt gccgatatgt ccgatttgca gaagtccgca cgcctcggac agcccgacac
    57481 gacagtggcg caatggctga aacagcggaa cgtgccgcgt gccgccgtga tgcagttttg
    57541 gcagccgctg gtgtggggtg cgctcaacac gcctttggaa accgcaagcc tgcgcgtgtt
    57601 gtgcaacgtt ttgtccgacg gcgtgctgac gaaaaaatcc ggcagcgact atctcctacc
    57661 caagcaggat ttgggcgcaa tcgtcgccga acccgccttg gcggagcttc aacggctcgg
    57721 cgcggacatc cgcctcgaaa cgcgcgtatg ccgtctgaac accctcccgg acgggaaagt
    57781 gctcgtcaac ggcgaagctt tcgatgccgc cgtccccgcc accgcgccct accacgccgc
    57841 cgcgctcctg cccgaaggca cgcccgaaca cgttcagacg gcatatcaaa accttcgcta
    57901 ccacgccatc accaccgtct atctgcgcta cgccgaaccc gtccgcctgc ccgccccgct
    57961 gaccggcctt gccgacggca cggtgcaatg gctgctttgc cggggcaggc tcggactgcc
    58021 tgaaaacgaa gtgtccgccg tcatcagcgt ttccgaccgc gtcggcgcgt ttgcaaaccg
    58081 ggcgtgggcg gacaaagccc acgccgacct caaacgcatc cttccgcatt tgggcgaacc
    58141 cgaagccgtg cgcgtcatca ccgaaaaacg cgccacaacc gcagccgatg ccccgccgcc
    58201 ggacttgtcg tggttgcacc ggcaccgcat cttccccgcc ggcgactacc tccacccgga
    58261 ctaccccgcc acgctcgaag ccgccgtaca atcaggtttc gcgtcggcgg aagcctgcct
    58321 gcaaagcctg atcgatgccg tctgaaaacg ccggccggca tcgggacgct ttacaaggtg
    58381 cggcaaaacg ctaaaatacc cccgaccgac caccaaaaag gaaaaagtat ggcgacactg
    58441 tccaacaaaa ccatcttagt taccggcgca tcgcaaggtc tgggcgaaca ggtcgccaaa
    58501 gcctatgcgg cggcaggcgc aaccgtgatt ctggtggcac gccatcagaa aaaactggaa
    58561 aaagtctatg acgcgattgt cgaagccgga caccccgaac ccttcgccat ccgtttcgac
    58621 ctgatgagtg cggaagaaaa agaattcaat cagttcgccg ccaccattgc cgaagccacg
    58681 caaggcaaac tggacggcat cgtccactgc gccggctatt tttacgccct ctcgccgctg
    58741 gatttccaaa ccgtcgccga atgggtcaac caataccgca tcaacaccgt cgcacctatg
    58801 gggctgaccc gcgccctgtt cccgctgctg aagcagtcgc ccgacgcgtc cgtcatcttc
    58861 gtcggcgaaa gccacggcga aacacccaaa gcctactggg gcggcttcgg cgcgtccaaa
    58921 gccgcgttga actacctgtg caaagtcgcc gccgacgaat gggaacgctt cggcaacctg
    58981 cgcgccaacg tcctcgtccc cggacccatc aattccccgc aacgcatcaa atcccatccg
    59041 ggcgaagcca aaagcgaacg caaaagctac ggggacgtgc tgcccgcatt tgtctggtgg
    59101 gcaagtaccg aaagcaaagg gcggagcggc gaaatcgttt acctctaaat ccggaaaagg
    59161 caggcatccc gcccgcgccg cgattccgtt tgaaaaatat ccgtaccttc cgcgcgcatc
    59221 cgtcccgcac agacaaacac cttgacttaa atttacataa ggacaataat gataacccgt
    59281 tatttcctta acttgacctt agggtaaatt ttatgaaaac cgtttccacc gccgttgtcc
    59341 ttgccgccgc tgccgtttca ctgaccggct gtgcgaccga atcctcacgc agtctcgagg
    59401 tagcaaaagt cgcctcctac aatacgcaat atcacggtgt tcgtaccccg atttccgtcg
    59461 gaacattcga caaccgctcc agcttccaaa aaggcatttt ctccgacggg gaagaccgtt
    59521 tgggcagcca ggcaaaaacc attctggtaa cgcacctgca acagaccaac cgcttcaacg
    59581 tactgaaccg caccaatttg aacgcattaa aacaggaatc cggcatttcc ggcaaagcgc
    59641 ataacctgaa aggtgcagat tatgtcgtta ccggcgatgt aaccgaattc ggacgcagag
    59701 atgtcggcga tcatcagctc ttcggcattt tgggtcgcgg caaatcgcaa atcgcctatg
    59761 caaaagtggc tctgaatatc gtcaacgtca atacttccga aatcgtctat tccgcacagg
    59821 gcgcgggcga atacgcactt tccaaccgtg aaatcatcgg tttcggcggc acttccggct
    59881 acgatgcgac tttgaacggc aaagttttag acttggcaat ccgcgaagcc gtcaacagcc
    59941 tggttcaggc tgttgacaac ggcgcatggc aacccaaccg ttaaggagaa atgatgaatc
    60001 cgaaaacctt gagccgtttg tcgctgtgtg cggcggtctt ggctctgacc gcctgcggcg
    60061 gcaacgggca aaaatccctg tattattacg gcggctatcc cgataccgtc tatgaaggtt
    60121 tgaaaaacga cgacacttcg ttgggcaagc agaccgaaaa gatggaaaaa tactttgtgg
    60181 aagccggcaa caaaaaaatg aatgccgccc cgggtgcgca cgcccatctg ggactgctgc
    60241 tttcccgttc gggagacaaa gagggcgcgt tccgccagtt tgaagaagag aaaaggctgt
    60301 ttcccgaatc gggcgtattt atggacttcc tgatgaaaac cggtaaagga ggcaagcgat
    60361 gaaacctttg attttagggc ttgccgccgt gttggcgctg tctgcctgcc aagttcaaaa
    60421 agcgcccgat ttcgactaca cgtcattcaa ggaaagcaaa ccggcttcaa ttttggtggt
    60481 tccgccgctg aacgaatcgc ccgatgtcaa cggaacatgg ggtgtactgg cttcgaccgc
    60541 cgctccgctt tccgaagccg gctattacgt cttccccgcc gcagtcgtgg aggaaacctt
    60601 caaagaaaac ggcttgacca atgccgccga tattcacgcc gtccggccgg aaaaactgca
    60661 tcagattttc ggcaatgatg cggttttgta cattacggtt accgaatacg gcacttcata
    60721 tcaaatttta gacagcgtga cgaccgtatc cgccaaagca cggctggtcg attcccgcaa
    60781 cggaaaagag ttgtggtcgg gttcggccag catccgcgaa ggcagcaaca acagcaacag
    60841 cggcctgttg ggggctttgg tcagcgcagt ggtcaatcag attgccaaca gcctgaccga
    60901 ccgcggttat caggtttcta aaaccgccgc atacaacctg ctgtcgccct attctcacaa
    60961 cggcatcttg aaaggtccga gattcgtcga agagcagccc aaataagcat atgccgtccg
    61021 aacctctccg agcttcagac ggcatatgcg aatcttgata cttgcaagcc gcaccgccca
    61081 aaatcgggcg gccggtttca atatccgaac aaggttcggg ttctgtattt tgcagaaccc
    61141 gaacctttta ttcaaattta aacttaattc cggcgtggtt gtatggtata gtggattaac
    61201 aaaaatcagg acaaggcgac gaagccgcag acagtacaaa tagtacggaa ccgattcact
    61261 tggtgcttca gcaccttaga aaatcgttct ctttgagcta a
//
LOCUS       DAWFHY010000002        45394 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.002, whole genome shotgun sequence.
ACCESSION   DAWFHY010000002 DAWFHY010000000
VERSION     DAWFHY010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 45394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45394
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.002"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(1..83)
                     /locus_tag="WGQ68_000070"
                     /pseudo
     CDS             complement(1..83)
                     /locus_tag="WGQ68_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006248742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
     gene            complement(188..685)
                     /locus_tag="WGQ68_000071"
     CDS             complement(188..685)
                     /locus_tag="WGQ68_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter"
                     /protein_id="HEZ0713959.1"
                     /translation="MNVNVHKDTHHFGIHNLNNTDVHLTAEDSDNVIEIKGELADPMP
                     TGNVESTSFLRGIETAFKSNTVLTGNNNKISIATEGKGLGLGVNGLQSNSPYSGHTTG
                     IFINNQSSDLTQPSLDGISTIKLKAVKGNNEVDLNVKNHASVKGIIASHSAKATLEAE
                     GDNIV"
     gene            complement(779..1192)
                     /locus_tag="WGQ68_000072"
     CDS             complement(779..1192)
                     /locus_tag="WGQ68_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713960.1"
                     /translation="MEWVSLFSDPQRDDDSLITLKDEKITVKNYIVPWWKKGENFRKL
                     ELITTGKNDNGNLTVNGIYGIQSFADSQLGYTPELLVKSNNDILFKNNLQSVDVKPHS
                     GNIHLDAKGKIQFNLNVPDVDGVQDVASEKNTDKQ"
     gene            1504..2814
                     /locus_tag="WGQ68_000073"
     CDS             1504..2814
                     /locus_tag="WGQ68_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEZ0713961.1"
                     /translation="MTDLFAREPDAPLAERLRPHTLDDVVGQEHLIGEGKPLRVAVEG
                     GKPHSMLLWGPPGVGKTTLAQILAQSFNAQFLPVSAVFSGVKDIREAIDKAEIALQQG
                     RATILFVDEVHRFNKAQQDAFLPHVESGLLTFIGATTENPSFEVNPALLSRAQVYVLQ
                     PLSSDDLKKLIAKVLALPEYQEFTIETDAQKLLVNTADGDARRLLNLLEQLLRAADTR
                     RLKNLTAEFLADSLGAQIRRFDKGGESFYNQISALHKSVRGSHPNAALYWFCRMLDGG
                     TDPRYLARRIVRIAWEDIGLADPRAFQIANDAAATFERLGSPEGELALAQAVLYLAAA
                     AKSNAGYKAYNQMRRFVKENASDEVPVHLRNAPTKLMKELGYGREYRYAHDEPNAYAA
                     GESYMPDGLDEPDFYQPVPRGLEIKIGEKLAWLKSLDEEALKAK"
     gene            2928..5933
                     /locus_tag="WGQ68_000074"
     CDS             2928..5933
                     /locus_tag="WGQ68_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEZ0713962.1"
                     /translation="MSQTHNENSRVKIPAVLHLMRLGYDYLSLKNENWDKPTNIFPKI
                     FIESLCRINPDLSPDDARRLLTDIRLELDNEDLGQKFYERLTNQSGGKKLIDFQNFDN
                     NSFHVVTELPCINGDEEFRPDITLLVNGMPLVFIEVKKPNNKGGIGEERERMGKRAKN
                     SKFRRFINITQFMIFSNNMEYDDGATEPAQGAFYASSAYGKPVFNYFREEHKLNLAEL
                     LNTLSDGLENNVLQDNNLPVIKHSPEFISNKSPDTPTNRILTSLLCRERLSFLLQHGL
                     TYVKASQGLVQKHIMRYPQLFATLAIEKHLANGGKKGVIWHTQGSGKTALAYYNTRYL
                     THYYAKQGIVPKFYFIVDRLDLLKQAQREFTARDLVVHTIDSREAFAADIKSAQTLHN
                     HAGKAEITVVNIQKFQDDPDVVARNDYDLAIQRVYFLDEVHRSYNPKGSFLANLNQSD
                     VNAVKIGLTGTPLIGVTAGNVNTRELFGDYIHKYYYNASIADGYTLRLIREEIGSRYK
                     AQLQEALAQLEIEKGSFDRKEIYAHPHFVHPMLDYILDDFAKFRKTNQDDSLGAMVVC
                     DSAEQARQLFEHFQIASDHNFTAALILHDVGTKDERDQWVKDFKAGKIDILFVYNMLL
                     TGFDAPRLKKLYLGRLIKAHNLLQTLTRVNRTYKSYRYGYVVDFADIEREFDKTNRAY
                     WDELSNELGDEIGSYSRLFKTAEEIEQEIADIKNALFDFDTENAEEFCSQISQIADKK
                     QLLALKKALQTAKKLYNVLRLQGSHEFLAHLDFDKLNLLYRETAARLETLNLAEKLQQ
                     GDTAHLLNEALEDVYFQFVKIGEAELKLADDLKDIMRKVREGLAGNFDQEDPEYISLR
                     EELERIFKKKNLTEVGQEEMQANIATLDTVYAQIKELNRKNDLLRHKYGGDTKYARIH
                     KRLLENPALYGDKLKVFNALNGVKTDADQKVLDMEQILDNQNYFEKQMQGIVLKRFRT
                     EQQFPVQPADIQAINRLLVREYLKESGRI"
     gene            5982..6971
                     /locus_tag="WGQ68_000075"
     CDS             5982..6971
                     /locus_tag="WGQ68_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004143539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0713963.1"
                     /translation="MYNNDIIIYTTEDGLSEFTLRELDGELWLTQKEIAELYQTSKQN
                     IGKHIKAIFAEQELDDSVVNFQFTTAADGKNYRMGLYPLSLIIAVGYRVRSTRGTQFR
                     QWATRTLGEYISKGFVLNDDRLKNPPVGTNRAEDQFEQLLNRIRDIRSSERRMYLRVR
                     EIFAMAADYQPSFKETTHFFKIIQNKLHYACTRYTAPEIIYQRADAGKPNMGLTHWRG
                     KEIAKADVTVAKNYLNEAEIDALNRIVSMWLDFAEDQASMKKQIFLQDWAEKLDAFLA
                     FNDRQVLQTAGTISKTQADEKARIEYEKFAAARRLEKEREGERHIAELLNLKK"
     gene            7065..8222
                     /locus_tag="WGQ68_000076"
     CDS             7065..8222
                     /locus_tag="WGQ68_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0713964.1"
                     /translation="MNSALKNHATYINNKVDTNEISLSQYISTDNILQNKQGIECATS
                     LPIQGGKVTSFKKGDILLANIRPYLKKIWYAQFDGGCSADVLAIRANTETDSHFLFYA
                     LFRDDFFIHAMKGSKGTKMPRGDKTQIMEFKIPVFAPQTQQSITTVLSALDKKIALNK
                     QINARLEEMAKTLYDYWFVQFDFPDANGKPYKSSGGEMVFDETLKREIPKGWESVELQ
                     SCLAKVPSTVKISNKDIKDFGKYPVIDQSQDFICGFTDDEKSILNPQDAHIIFGDHTR
                     IVKLVNFKYARGADGTQVILSNNERMPNYLFYQIINQIDLSSYGYARHFKFLKEFPII
                     LPDKDISRKYYEIVNYFFIKIRNNIKQNHHLTQLRDFLLPMLMNGQVSVAE"
     gene            8271..9872
                     /locus_tag="WGQ68_000077"
     CDS             8271..9872
                     /locus_tag="WGQ68_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0713965.1"
                     /translation="MTEQHFTEQTKALIDSLKTICANYGLGNDGNEFKIISQAFLYKF
                     LNDKYDFEVKKIRKENPDEEIEFINMDIEGKTAVLKPEHSIKYLSERQNGADFAKLFD
                     DTLTDIAAHNAELFSVKTEGGAKIVLFERISQYITDEGRRDDFCRALISKLAGFSFEV
                     IFAQKFDFFATIFEYLIKDYNSNSGGKYAEYYTPHAVARIMADILVPEEVRGQIRSVD
                     VYDPSAGSGTLLMNVAHAIGEDKCMIYTQDISQKSSNLLRLNLILNNLVHSLNNVVQG
                     NTILSPYHKDASGRLKKFDFIVSNPPFKLDFSDFRDQLESDENRERFFAGIPKIKAKD
                     KDKMEIYQLFIQHILFSLKENGKAAIVLPTGFITAQSGIDKKIREYLVENKMLAGVVS
                     MPSNIFATTGTNVSILFIDKANKDKVVLIDASGLGEKIKDGKNQKTVLSREEEQKICN
                     TFTHKQAVEDFSVVIGYDEIKAKNHSLSAGQYFEVKIDYVDISAEEFEQKMAGFSADL
                     DKLFAESAELEKEIKDRLAMLKFNS"
     gene            complement(9968..11305)
                     /gene="ribB"
                     /locus_tag="WGQ68_000078"
     CDS             complement(9968..11305)
                     /gene="ribB"
                     /locus_tag="WGQ68_000078"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEZ0713966.1"
                     /translation="MTDTAGLRRHNLRQWIEKYYGGLQTRFAEAVALNTGELSALLKN
                     KSFGEKKARKIEQAAKMPAFWLDTEHTARPSEHTGKHTMSHISPIPEILADIKAGKMV
                     IITDAEDRENEGDLLMAAQFVTPEAINFMIKHARGLVCLPMDGEMVEKLGLPMMTQKN
                     GAQYGTNFTVSIEAAHGITTGISAADRALTIQTAVSPTAKPEDIVQPGHIFPLRAQKG
                     GVLVRAGHTEAGVDLAQMNGLIPASVICEIINDDGTMARMPELMKFAEEHKLKIGTIA
                     DLIEYRSRTESLLEDMGNAPVQTPWGEFQQHVYVDKLSGETHLALVKGTPSADTETLV
                     RVHEPFSVMDFIQANPRHSWSLPKALERVQQAESGVVILLHRTEDGASLLDRTLPKGA
                     NQAYKWDSKSYGIGAQILAGLNVKKLRVLGQPSSFTGLTGFGLEVVGFEEAEK"
     gene            complement(11521..12534)
                     /locus_tag="WGQ68_000079"
     CDS             complement(11521..12534)
                     /locus_tag="WGQ68_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0713967.1"
                     /translation="MIFSIIVPIYNVEKYLRCCVDSVLAENFADYEMILVDDGSPDGC
                     GKICDEYAGKYPHIKVIHQENGGLSDARNAGIRAAKGDYLIFLDSDDYWADTNRSKNA
                     GGGILFDLQQLADKKVDLILHPSSLNYHVIPKGADFSDNDFVRHFETLVEGRYYIANA
                     WTKIVRREIIIKNNLFFPKGYIHEDFPYNLQLARFIKTFAFYDNPFYQYRVLGGSISH
                     NIKYKNFSDVLTHLDWGVDFLVENKNSPIYGGLQKFVFDNIGYLRSILVRLYFSKNII
                     LIYRKYFSFKEKCRKIFGAKAIRPVFIGKTAFIIGLPILRLLVPPMLYPAIKAVYQKF
                     FSE"
     gene            complement(12702..13295)
                     /gene="ribA"
                     /locus_tag="WGQ68_000080"
     CDS             complement(12702..13295)
                     /gene="ribA"
                     /locus_tag="WGQ68_000080"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEZ0713968.1"
                     /translation="MSEMLNHVASCRLPTEWGVFTMHGFEEANGQEHVALTVGDFSDG
                     NPVLTRIHSECLTGDALFSRKCDCGPQLEAAMRAVQTEGRGIIVYLRQEGRGIGLINK
                     IRAYHLQDQGMDTVEANLALGLPVDARDFRLAQSIYEYLGIRSVKLLTNNPEKIQTLK
                     DAGINVVERIPLHVGENLENKRYLQTKADKLGHLMSE"
     gene            complement(13288..13965)
                     /locus_tag="WGQ68_000081"
     CDS             complement(13288..13965)
                     /locus_tag="WGQ68_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713969.1"
                     /translation="MPAALIKDFLLTQGLKLPLDEVRAEYLTAQTVMDMGTASIERSV
                     LWLEHEDWRLADYLEQNSGNEEGLKRLFMALDSVFSRCKGVRAAAVYALMPSENAAFR
                     LVCLSRQGEVLENLWDLDEAAGKVSLACRSAQSGWMNIASDVRRWLDLGELSGERNHA
                     SAAQISIPVCTESGGVLGVVHVEFECAECAGTAAQVEWVALALALSEPLKLLLGMSAG
                     KDGSEDV"
     gene            14119..14203
                     /locus_tag="WGQ68_000082"
     tRNA            14119..14203
                     /locus_tag="WGQ68_000082"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14153..14155,aa:Leu,seq:tag)
     gene            14723..15757
                     /gene="purM"
                     /locus_tag="WGQ68_000083"
     CDS             14723..15757
                     /gene="purM"
                     /locus_tag="WGQ68_000083"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEZ0713970.1"
                     /translation="MSTSLSYRDAGVDIDAGDQLVENIKPFAKRTMRPEVLGDLGGFG
                     ALVEIGKKYKNPVLVSGTDGVGTKLKLAFDWDKHDTVGIDLVAMSVNDILVQGAEPLF
                     FLDYFACGKLDVPRATDVIKGIAQGCEESGCALIGGETAEMPGMYPVGEYDLAGFAVG
                     VVEKENVITGRSIGAGDVVLGLASNGAHSNGYSLIRKIIERDNPDLDAEFDNGKTLRE
                     AVIAPTRLYVKPILAALEKFTIKGMAHITGGGITENVPRVLPENTVAQIDAKSWELPK
                     LFQWLQKAGNVETQEMYRTFNCGIGMVVIVAAEDADAVQGLLGEQGETVYRLGLIRER
                     QGDEHQTQVA"
     gene            complement(16231..16887)
                     /locus_tag="WGQ68_000084"
     CDS             complement(16231..16887)
                     /locus_tag="WGQ68_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0713971.1"
                     /translation="MTIKIILIDDHTLFRSGIKALLSRQHDFEVIGEAADGLSGIKMI
                     NRLQPDVVLLDLDMPGMNGREALSQIISINPQQAVIMLTVSEDNDDLTECMRIGARGY
                     LLKNINADFLLESIRKAAEGDNVFSPEMTAKLVKSLISPQPAQGTQALSSLTPRELEI
                     LGYLAAGHSNKIIARHLDLAESTVKVHVQNLLRKLNLSSRVQAAVYAIRHNVPQPVLE
                     "
     gene            complement(16884..18656)
                     /locus_tag="WGQ68_000085"
     CDS             complement(16884..18656)
                     /locus_tag="WGQ68_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite sensor histidine kinase"
                     /protein_id="HEZ0713972.1"
                     /translation="MILPARFSDGISLSLRLKLLTGLWVGLAALSVVLTLLLSLRLEN
                     AASVIEEAGNLRMQAYRLAYMAGEGSPRSQIDNQVDEFEKSLNRISQSDAIHPLIPSD
                     TPLAYDLIQSMLIIDWQAHILPPLQSYRRPTQVDLYRFAGNIELFLQALENANEKNTW
                     WLRRFQWAIMLMTLVSSVLMLFWHQIWVIRPLQALREGAERIGRRCFDIPVPEGGTPE
                     FKQVGRCFNQMGGRLKILYDDLEGQVAEQTRSLEKQNQNLTLLYQTTRDLHQSYIPQQ
                     AAEHFLNRILPAVGADSGRVCLDGGSDVYVSIHHADCGTAASDLGKYHEEIFPIEYQN
                     ETLGRLLLSFPNGISLDEDDRILLQTLGRQLGVSLAGAKQEEEKRLLAVLQERNLIAQ
                     GLHDSIAQALTFLNLQVQMLETAFAENKREEAAENIGFIKTGVQECYEDVRELLLNFR
                     TKISNKEFPEAVADLFARFTQQTGTTVETVWENGTHLPTQDEQLQMIFILQESLSNIR
                     KHARATHVKFTLSEHGGRFTMTIQDNGQGFDTEKIGEPTGSHVGLHIMQERAKRIRAV
                     LKIYSQPQQGTTVSLAGVSEESLK"
     gene            18862..19476
                     /locus_tag="WGQ68_000086"
     CDS             18862..19476
                     /locus_tag="WGQ68_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEZ0713973.1"
                     /translation="MKTSNFSKRTSAMKTFALILADGQASRMGGEDKGLALLGGKALI
                     DHVIDRVRPQVSHIAISTNRNLEEYARRSPHIFPDARQWQHFGPLSALCTAANDLQLA
                     TADWLLVVPCDMPYLPGDLVARFETVSKRTPLCNAYYVETPITMHYNIMYIRPQILQS
                     AIPYLFSGMKTLRSWLQQQRARPVRFEFDGHFADLNTQIDLQEG"
     gene            19639..20256
                     /locus_tag="WGQ68_000087"
     CDS             19639..20256
                     /locus_tag="WGQ68_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEZ0713974.1"
                     /translation="MFTGIVQGLGKLTAIHRPSEAFHTYVVELPQEAADNLQRGASVA
                     NNGCCLTITEIEGNRVSFDLMAETLAKTNLGLLKEGDCVNIERAARFGDEIGGHVMSG
                     HIMATVPIVEIERDGFNRTVWFSLPHELKPYILTKGFVGLDGCSLTIGKVEDGRFNVH
                     LIPETLERTLFGSRKVGDRINIEIDPNTQAIVDTVERLMAQRYAK"
     gene            20264..21127
                     /locus_tag="WGQ68_000088"
     CDS             20264..21127
                     /locus_tag="WGQ68_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0713975.1"
                     /translation="MELNEFLDKAYAVLRRLDAVLPPEPGHTDWNALAFRWQSAGKKG
                     FLEHLPDPHTFPLVRLAGVGRQTELLVRNTEQFIVGRPANNVLMSGARGTGKSSLVKA
                     LLHEYADKGLRLIEVDKSDLIGLPYLLTLLKERPEKFIVFCDDLSFESGDETYKALKT
                     ALDGGLSQRCANVLVYATSNRRHLMPEYFDENAGTTGMRGEIHQKEAVEEKISLSDRF
                     GLWLSFYPFDQNDYLAAVQNWLEDFGVPYDETAQMAALQWAQTRGSRSGRSAWQFACD
                     WAGRLPKQRAL"
     gene            complement(21140..21574)
                     /locus_tag="WGQ68_000089"
     CDS             complement(21140..21574)
                     /locus_tag="WGQ68_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEZ0713976.1"
                     /translation="MPLPAPCRFAKPAASFLSMALLSCQLSHAATAYIPPNDFQPNCD
                     IRRLGLTQSQHNELRKIRTAFKMAGDRARLKVMHSEHSRRRSVVEIISSDVFNRNEAR
                     DYVESRYLSGMDFAVDELEIQHRFFHILTPQQQQMWLSSCLK"
     gene            21720..22448
                     /gene="rpe"
                     /locus_tag="WGQ68_000090"
     CDS             21720..22448
                     /gene="rpe"
                     /locus_tag="WGQ68_000090"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEZ0713977.1"
                     /translation="MTAYRIAPSILSADFARLGEEVESVIAAGADLIHFDVMDNHYVP
                     NLTFGPMVCAALKPYASVPIDVHLMVEPVDDLIQSFAKAGASIITFHPEASRHIDRSL
                     SLIRDMGCQAGLVLNPATPVYLLENVLDRLDMVLLMSVNPGFGGQSFIPHTLEKIRRV
                     RAMLDRYEAQSGRHIAIEVDGGIKTDNIAAVARAGADTFVAGSAIFGKPDYKAVIDAM
                     RAELEKSGRLNLAPMIEIEMPSEN"
     gene            complement(22471..23502)
                     /gene="ruvB"
                     /locus_tag="WGQ68_000091"
     CDS             complement(22471..23502)
                     /gene="ruvB"
                     /locus_tag="WGQ68_000091"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEZ0713978.1"
                     /translation="MLQTDNLTAAQPQRIVAAQTASAQEELLERALRPKTLDDYIGQD
                     KAKEQLAIFIQAAKKRGEALDHVLLFGPPGLGKTTLAHIIAKELGVNLRQTSGPVLER
                     AGDLAALLTNLDPHDVLFIDEIHRLSPVVEEILYPALEDYRLDIMIGEGPAARSVKID
                     LPPFTLIGATTRAGMLTNPLRDRFGIVSRLEFYENRDLTTIVSRSAQLLQLDMSEEGA
                     EEIAKRSRGTPRIANRLLRRVRDFADVKNNGTIDGGIADAALSMLDVDAQGLDVMDRK
                     FLEAVLHKFGGGPVGLDNVAAAIGESTDTIEDVIEPYLIQQGFLQRTPRGRMATERAY
                     LHFGLPVEK"
     gene            complement(23584..23892)
                     /locus_tag="WGQ68_000092"
     CDS             complement(23584..23892)
                     /locus_tag="WGQ68_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713979.1"
                     /translation="MTLKTDLLPKINNEDYQRLILKHSAEFSGGEIRLLNEILEKFNF
                     DVVQAQALAQAVMQQIRFDPNAYHIDSDDEDTTGICPHCINPPMPPLRDYLVWRETRG
                     "
     gene            complement(24115..25368)
                     /locus_tag="WGQ68_000093"
     CDS             complement(24115..25368)
                     /locus_tag="WGQ68_000093"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HEZ0713980.1"
                     /translation="MQTYLVGGAVRDYLLGLPVKDRDWVVVGADAQTMLAQGFQPVGK
                     DFPVFLHPETHEEYALARTERKTAKGYVGFSFHADKDVTLEQDLMRRDLTINAMAQDA
                     DGKIIDPFGGQRDLAAGILRHVSPAFAEDPVRILRTARFAARYRFEIAEETIKLMRQM
                     VENGEADALVAERVWQEFAKGLMEKNPRKMIEVLRECGALKVLLPEVNALFGVPQRAD
                     YHPEIDSGIHTLMTLQRAADMGLSLPERYAALLHDLGKAKTPSDILPRHHGHDLAGVE
                     PVREVNQRLRAPKHCAELAELVCRWHIIFHQVGQLKSQTILNVLKKTDAFRRPERFQT
                     ALNVCIADTQGRLNREHTPYPQRAHWFALLEAANQADSGKIAAECRAQGKAHLIAEQI
                     DRARLAQIAPLQKTFRAAQDKTEKH"
     gene            complement(25440..27128)
                     /locus_tag="WGQ68_000094"
     CDS             complement(25440..27128)
                     /locus_tag="WGQ68_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HEZ0713981.1"
                     /translation="MLQSENSKNLISCSIKALPIMISTNGITMQFGAKPLFENVSVKF
                     GEGNRYGLIGANGSGKSTFMKILGGDLEQTAGEVAIENGVRLGKLRQDQFAYEEMRVL
                     DVVMMGHTEMWAAMTERDAIYANLEATEDDYMKAAELEAKFAEYDGYTAEARAAELLS
                     GVGISEDLHNATMAEVAPGFKLRVLLAQALFSKPDVLLLDEPTNNLDINTIRWLEGVL
                     NQYDSTMIIISHDRHFLNEVCTHMADLDYNTITIYPGNYDDYMLASAQSRERALKDNA
                     KAKEKLQELQEFVARFSANKSKARQATSRLKQADKIKSEMVEVKPSTRQNPYIRFEAD
                     EKAKLHRQAVEVEKLAKRFETQLFKNLNFILEAGQRLAIIGPNGAGKSTLLKLLAGAY
                     NPEYSDGLLPDEGTIKWAEKASVGYYPQDHENDFDVDMDLSEWMRQWGQEGDDEQVIR
                     GTLGRLLFGSNDVVKKVKVLSGGEKGRMLYGKLLLLKPNVLVMDEPTNHMDMESIESL
                     NMALEKYNGTLIFVSHDRQFVSSLATQIIELDGKGGYEHYLGDYESYLEKKGVA"
     gene            27218..27592
                     /locus_tag="WGQ68_000095"
     CDS             27218..27592
                     /locus_tag="WGQ68_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEZ0713982.1"
                     /translation="MIVLHGIPNCDTVKKAKNRLAEYGMEFEFRDFKKQVPSEAEICS
                     WLEQVPLATLLNKRGTSWRKLDAETQQKVLSSTAEAVKLMSEMPSLIKRPVLECGGKV
                     YAGFSEETYDGIFNRQAPCRQG"
     gene            27652..29190
                     /locus_tag="WGQ68_000096"
     CDS             27652..29190
                     /locus_tag="WGQ68_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEZ0713983.1"
                     /translation="MFHSIEKYRTPAQVLLGLIALTFVGFGVSTVSHPGADYIVQVGD
                     EKISDHSINNAIQNEQADGGGPSRDAVFQSLLQRAYLKQGSKLMGISVSSEQIKQIIV
                     EDPNFHDAGGKFDHALLNRYLSQRHMSEDQFVEEIRDQFALQNLVNLVQNGVLVGDAQ
                     AEQLIRLTQVNRTIRSHTFNPDEFIAQVKVSEADLQKFYNANKKDYLLPQAVKLEYVA
                     LNLKDFADKQAVSETEVKNAFEERVARLPANETKPSFEQEKAAVENELKMKKAVADFN
                     KAKEKLGDDAFNHPSSLAEAAKNSGLKVETQETWLSRQDAQMSGMPENLINAVFSDDV
                     LKKKHNSEVLTINSETAWVVRAKEVREEKTLPFAEAKDAVRQAYIRTEAAKLAENKAK
                     EVLAQLSGGKAVDVKWSEVSVLGAQQARQSMPPEAYAELLKAKPANGKPAYVRLTGLP
                     APVIVEVQAITPPDDIAAQLPLAKQALAQQQSANTFDLLIRYFNGKIKQTKGAQSVDN
                     GDGQ"
     gene            29257..29874
                     /gene="recR"
                     /locus_tag="WGQ68_000097"
     CDS             29257..29874
                     /gene="recR"
                     /locus_tag="WGQ68_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEZ0713984.1"
                     /translation="MSHKKQDAFQGLIDALKVLPNVGPKLAQRIAYHLLQHKRKEAEK
                     LVDALQTALKQVYHCAMCNTFCEGGLCDICADETRDGRRLMVVHMPADVSNMEAANCH
                     DGLYFVLMGQINTALGMDVSAIALDRLAQRLGGGEVEEIIIATAFTAEGNATAYVLSE
                     FFKNLPYKVSRLSQGIPLGGELEYVDAGTLAQAVYERRLIKEGGA"
     gene            complement(29930..30208)
                     /locus_tag="WGQ68_000098"
     CDS             complement(29930..30208)
                     /locus_tag="WGQ68_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713985.1"
                     /translation="MNIRTAFALCAIALSAAAAAYAKEIKIDANNTPYSEADAQKLAA
                     TAVGMGVKEPISLNGGSGSITVSGSSATQCVFKVGNGGALQIQGLNCK"
     gene            30413..31660
                     /locus_tag="WGQ68_000099"
     CDS             30413..31660
                     /locus_tag="WGQ68_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEZ0713986.1"
                     /translation="MFSLEAWIGLRYLRAKKRNGFMSFITMVSIAGIALGVTALIVVL
                     SVMNGFQKEIRGQLLNVAPHAEIGYIDNTDTDWRNLLRFTENRKGILAAAPYVSNQAL
                     LANAGEIRGVQMRGILPSEERKVVEYGDKMPAGKFEDLIPGEFDIILGVGLAEALGAE
                     VGNKVTVITPEGNVTPAGVVPRLKQFTVVGLVKTGVYEVDNSLAMTHIQDARVLYRLD
                     KEVAGLRLKLADPQNAPALTATLIPEAQRDAVWVRDWTYNNRSYFEAVELEKRMMFII
                     LTLIIAVAAFNLVSSLVMAVTEKQADIAILRTLGLSPGGVMKIFMVQGAFSGFFGTLA
                     GVVCGVLLGWNVGRVVAFFENLLGVHLINSQVYFIDYLPSDVDMGDVALIACISLGLS
                     FVATLYPSRRASKTQPAEALRYE"
     gene            31653..32348
                     /gene="lolD"
                     /locus_tag="WGQ68_000100"
     CDS             31653..32348
                     /gene="lolD"
                     /locus_tag="WGQ68_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HEZ0713987.1"
                     /translation="MSELILKCEGVGKRYRDGGLDVRVLHGLDLEIRAGESTGIIGSS
                     GSGKSTLLHILGGLDMPSEGRVLLMGEDLRTLNQRRLGDLRNRHLGFVYQFHHLLPEF
                     SALENVMMPLLIGKKSREEAAEAAMAMLEKVGLKHRSTHRAGELSGGERQRAAIARAL
                     VTQPKCLLADEPTGNLDRANARNVLDMMLELKTELGTGLVVVTHDDELAGRFGRVMVM
                     KDGSLHPKQGANA"
     gene            32416..34161
                     /gene="recD"
                     /locus_tag="WGQ68_000101"
     CDS             32416..34161
                     /gene="recD"
                     /locus_tag="WGQ68_000101"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HEZ0713988.1"
                     /translation="MERQTDEFAQAAARAAIRFLEHYAGSGDEVLANCTERLFQALQN
                     GHSFIRLSGGEADALSALAPVVGTSAAPLILEGRRLFLGRMWQLEYDLAAEIKRLAAA
                     GTSAPDATGARQNLAKWFQGAGSEGQRDAAALALLQSFMVITGGPGTGKTTTVAKLLA
                     LICGEDENLPHIALAAPTGKAAAHMARALHRAINGFDAPEAVRRHLLKLEGQTVHRLL
                     KLRPPKMQAAFDHIRPLPFDVLIVDEASMLDTALMLQLLKAVKTGARVILLGDENQLP
                     SVGIGAVLSVLSQKTVLDGETHQRLAGFLPEHGFSVSANPPVLAQNTAHLSFSHRFGD
                     NSGIGCLARAAVSGDEGAWALFDRFPDELEHSECSPNARVERLYRAHKAYWQAVKDGN
                     IEAAYAGISDIVVLAAWRQDAEDFNEAYCRHVRRKMNIPEHQAYFAGEPIMIRQNDYA
                     LELFNGDIGLIMEDVGRQGSLAAYFADADGFKKVAVSCLPDFEPAFAMTVHKSQGSEY
                     REVWLLPPSAAPSDEGDDALSGLSRGLLYTAITRAREKFVFFGGEEAFRQAAVTVKTR
                     QTALGSMLERVFSQE"
     gene            complement(34189..35292)
                     /locus_tag="WGQ68_000102"
     CDS             complement(34189..35292)
                     /locus_tag="WGQ68_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0713989.1"
                     /translation="MSSFIFPDNGFEWRNESLQKPPKGWHYVRESGANGILKAAYQNI
                     ATVWQGDFHNAKQVLSAMKKRVRKPAAVRSDADIAALFHAHRMKQAQQSRILNMLAVE
                     IRPGFVLDNKRAPDIRAALLDVYGEADGKPFFLPLNLLLGFMGAHEWHKKGVAVPQLG
                     GSIHVPFGVFSPLRGEYLDLLAHAPSTGFQTAFDIGTGSGVLAAILAKQGIPSVIGTD
                     TNPKAVACARANIARLGFEKQVEIRETDLFPEGFVDLIVCNPPWLPAKPTSAVESALY
                     DPESAMLAAFLRDAPKHLNPDGEIRLIISDLAEHLGLRPADFLEKAFIRAGLRVADMI
                     KTKPKHKKAANPSDPLAFARTRETTFLYRLKKA"
     gene            complement(35267..35458)
                     /locus_tag="WGQ68_000103"
     CDS             complement(35267..35458)
                     /locus_tag="WGQ68_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase"
                     /protein_id="HEZ0713990.1"
                     /translation="MEFDAMPSETFRLQTAWAWKDKAGTKNCSVLPLLAVPSVWFAVL
                     PPYFQNMTQVFHVFFYFSR"
     gene            35543..37993
                     /gene="lon"
                     /locus_tag="WGQ68_000104"
     CDS             35543..37993
                     /gene="lon"
                     /locus_tag="WGQ68_000104"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEZ0713991.1"
                     /translation="MTQKEKHFEEYAALATLPLRDVVVYPHMVLPLFVGRPKSIAALE
                     NAITREEPVFLLAQTDAAVEEPVAADLYQTGTVAQVLQVLKLPDGTVKVLVEGLYRGR
                     VLTIEDTGGLFVSHIETVVEEDTGGNTDLEAVRRTLLAQFEQYAKLNKKIPAEIIGSI
                     NGIAENSRLTDTVAAHLQLKLAQRQQILEIPEIGKRMEFLLAQLESELDIMQAEKRIR
                     GRVKRQMEKSQREYYLNEQIKAIHKELGEEDENGELDALEAGIKKAGMSKEAEEKCLS
                     ELKKLKMMPPMSAESTVVRNYIDTLLELPWKKKSRVSKDIAKAGLVLDTDHYGLEKVK
                     ERILEYLAVQKRMDKLKGPILCLVGPPGVGKTSLGESIAKATGRKYVRMALGGVRDES
                     EIRGHRRTYIGSMPGKILQNMAKAGVKNPLFLLDEIDKLGSDFRGDPASALLEVLDPE
                     QNNKFADHYAEVDYDLSDVMFIATSNSLNIPTPLLDRMEIIRLSGYTEDEKINIAMQY
                     LVPKQMKRNGVKEGELAIEESAVRDIIRYYTREAGVRSLDREIAKICRKVVMQITLDE
                     DSKTKKTGKAKPRAVKVNEKNLHDYLGVRRFDYGVAESENRIGQVTGLAWTEVGGELL
                     TVEAAALPGKGVIQCTGQLGDVMKESVSAAWSVVRSRAESVGLAPDFYEKKDIHVHVP
                     EGATPKDGPSAGIAMTLAMVSAFTKIPVRADVAMTGEITLRGEVLPIGGLKEKLLAAL
                     RGGIKHVLIPKDNLKDLEEIPENVKTGLTIHPVKWIDEVLALGLESQPEPWAEPSGAE
                     AAAESASKPKPRSRATKH"
     gene            38175..38444
                     /locus_tag="WGQ68_000105"
     CDS             38175..38444
                     /locus_tag="WGQ68_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEZ0713992.1"
                     /translation="MNKSELIEAIAQEADISKAAAQKALDATTNAVTTALKQGDTVTL
                     VGFGTFYVGERAERQGRNPKTGEPLTIAAAKTPKFRAGKALKDAL"
     gene            complement(38503..38937)
                     /locus_tag="WGQ68_000106"
     CDS             complement(38503..38937)
                     /locus_tag="WGQ68_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713993.1"
                     /translation="MTEPKHEMLTKEQVAARKKAKAKIRTIRIWAWVILALLALTALL
                     SQCAMSKPQAKQKIVESCVKNIPFAEKWQNDLRARGLDSNNTRLAVDYCKCMWEQPLD
                     RLSEKQIRSFGKLGAQEQLDLLGGANAFEARDKQCVADLKSE"
     gene            complement(38930..40237)
                     /locus_tag="WGQ68_000107"
     CDS             complement(38930..40237)
                     /locus_tag="WGQ68_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEZ0713994.1"
                     /translation="MKPVNIGLLGLGTVGGGTAAVLRDNAAEISRRLGREVRISAVCD
                     LSEEKARQICPSAAFVKDPFELVAREDVDVVVELFGGTGIAKDAVLKAIENGKHIVTA
                     NKKLLAEYGNEIFSLAEEKNVMVQFEAAVAGGIPIIKALREGLAANRIKSIAGIINGT
                     SNFILSEMREKGSAFADVLKEAQALGYAEADPTFDIEGNDAGHKITIMSALAFGTPMN
                     FSACYLEGISKLDSRDIKYAEELGYRIKLLGITRKTGKGIELRVHPTLIPESRLLANV
                     NGVMNAVRVNADMVGETLYYGAGAGALPTASAVVADIIDIARLVEADTAHRVPHLAFQ
                     PAQVQAQTILPMDEITSSYYLRVQAKDEPGTLGQIAALLAQENVSIEALIQKGVIDQT
                     TAEIVILTHSTVEKHIKSAIAAIEALDCVEKPITMIRMESLHD"
     gene            40410..40805
                     /locus_tag="WGQ68_000108"
     CDS             40410..40805
                     /locus_tag="WGQ68_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713995.1"
                     /translation="MMRRRGKKMLFEENPIDEHFAEYDRRTGEIRLSGRHFNKPVLLH
                     RDSAGLSKWQSLSELTPDNLLSDVKADDYPEILIIGTGAAQEFIHPKIMADFSRIGIG
                     VECMNTDSAFRTLVFLHSEGRRAWAWLQP"
     gene            40882..42804
                     /locus_tag="WGQ68_000109"
     CDS             40882..42804
                     /locus_tag="WGQ68_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0713996.1"
                     /translation="MIEIKNLTLQRGLKVLLDKANATVNPGQRVGLIGKNGTGKSSLF
                     ALIKGKITQDGGDVSIPKNWRLASVSQETPDLDISALDYVLQGDAELQAFQTALRQAE
                     AQNDGMKQAEYHAKLEEIDAYTAPARAAKLLNGLGFSQEEHSRPVKSFSGGWRMRLNL
                     AQALICRADLLLLDEPTNHLDLETVLWLENHLASLPCTQIIISHDRDFLNAATTQTIE
                     LSQQKLTQYGGNYDFYQNERAQRLAQQQAAYVKQQAQIKHLQSFIDRFKAKATKAVQA
                     QSRMKALAKLERVAPAHLDSEFSFEFYHPDHLPNPLLKLEHADLGYEGKTVLHDITLS
                     LESGARYGLLGVNGSGKSTFIKALAGKIDLLSGSIVRSEKLNIGYFAQHQLDTIRADQ
                     NPVWHIQQLSPEVREQEIRNFLGGFNFAGDMALQKTEPFSGGEKARLALAMIIWQKPN
                     LLLLDEPTNHLDLDMRHALTLALQSFQGALIIVSHDRSLLEATTDSFLLIDKGRLKNF
                     DGDLNDYRQWRLAQENAAAAPAASAQSQSRKDTKRIEAQIRQEKARRGKPIQQKIDRA
                     EKEMAQLSEIQTACEAFLAQEDAYLEANKEKLQNTLSLLAKVKTQLAQIEEVWLACQE
                     ELERIETEIEKQFAER"
     gene            43270..43785
                     /locus_tag="WGQ68_000110"
     CDS             43270..43785
                     /locus_tag="WGQ68_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0713997.1"
                     /translation="MKSKLPLILINFSLISSPLGANAAKIYTCTINGETVYTTKPSKS
                     CLSTDLPPIGNYSSERYIPPQTSEPTPSPSNGGQAVKYKAPVKTVSKPAKSNTPPPQQ
                     APVNNSRRSILEAELSNERKALTEAQKMLSQARLAKGGNINHQEINALQSNVLDRQQN
                     IQALQRELGRM"
     gene            complement(44075..44209)
                     /locus_tag="WGQ68_000111"
     CDS             complement(44075..44209)
                     /locus_tag="WGQ68_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF45 domain-containing protein"
                     /protein_id="HEZ0713998.1"
                     /translation="MTHLLERHRNARFMAHMDNFLPNWQSIKQQLNALELFAQIYNLT
                     "
     gene            complement(44351..45319)
                     /locus_tag="WGQ68_000112"
     CDS             complement(44351..45319)
                     /locus_tag="WGQ68_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0713999.1"
                     /translation="MAHPDYAAFKAGHLAKFAAWHTQNDLAVIQPGSRPGSRPGRLIR
                     KWSESLLDAFKPGSLIEEYDFYQILTDYWAETLQDDVYLIAQDGWKAVKNLAEITKES
                     DEAANLTVVFEETETGKKGKAKTKRISKKYRSEVIAPELVARRYFSDGIAKLEEKQSE
                     LERLSQELENHIEEHGGEEGALNDVLDAKGKLSAKLLKTALEESGIEEGERAVLQTTQ
                     TLMTQEKAAKDAVKTQIEALNLAVFKQFGRLSEAEIKQLAVQDKWLADLQSRIENRLE
                     NSIQQLISRLNTLEDRYRSPMAELAREVEKWQSKVNAHLENMGFGG"
BASE COUNT        11308 a        11389 c        11913 g        10784 t
ORIGIN      
        1 tgacgcaaat gccgttttgt acaattccaa aagttttcaa ttccgttaat gcgattttgc
       61 cgtttggcga aatgcgtact gttccagtcg tggattgaac ccccaccctg tatagttctt
      121 tcgaagcatt ggggtattgt tttttcaaag catcgattaa agccgtattg gtgtccggat
      181 ttttaaccta gacaatattg tccccttcgg cttcaagcgt tgcttttgca gaatgcgacg
      241 cgataatgcc ttttacggag gcgtggtttt ttacatttaa atcaacttca ttatttccct
      301 tgacggcttt gagtttgatg gtgctgatgc catcaaggga aggttgggta aggtcgcttg
      361 attgattatt gatgaaaatg cccgtcgtgt gtccggaata gggggagttg gactggagac
      421 catttacgcc caatcccaag cccttgccct cagtggcgat ggatatttta ttgttattcc
      481 ccgttaatac ggtattggat ttaaaagccg tttcaatgcc ccttaaaaaa cttgtactct
      541 caacatttcc tgtcggcata gggtcggcga gctccccttt aatctcaatc acattatcgc
      601 tgtcttcggc agtcagatga acatcggtat tgttgaggtt gtgaataccg aaatggtgtg
      661 tgtctttatg aacgttaacg ttcatattga acaaattgcc tgtttttgaa tgaaaggata
      721 tgctgccttt gtcattttca tcggggggct gatagtgttg acataccgaa ctaaggcact
      781 attgtttgtc ggtatttttt tcactggcta catcttgtac cccatcaaca tcgggaacat
      841 tcaaattaaa ttgaattttt cctttggcat ccaaatggat atttccggaa tggggcttca
      901 cgtcaacaga ttggagatta tttttaaata gaatatcatt attgcttttt accaataatt
      961 caggcgtata tcccaattga gagtctgcaa aagattggat gccgtaaatt ccatttaccg
     1021 ttaaatttcc attgtcattt ttaccagtgg taataagttc taattttcta aagttttcac
     1081 cttttttcca ccaaggcaca atatagtttt ttacagtgat tttttcatct ttaagggtta
     1141 taagactatc atcgtctctt tgcggatcag aaaataaaga cacccattcc atctcttgaa
     1201 tattagtata ttcatgggct tccgcctcaa aacataatgc cgattgaatc aacaaagcaa
     1261 tggtagataa agcaaatttt ctattttagt ctccttaatg tttaattgaa acgctgattt
     1321 gggtattgtc atgccgatgc ggaatcggtc tccgtgccgg atatattgtg tgaagtctct
     1381 cgtcttttcc agtatgcgcc taatatcggc ttaaggcaaa cggcggggcg gaatccgtat
     1441 gcggcacgcc gccgttccct gccgtataat catattgttt gaaaacctgt tgacggagcc
     1501 gttatgaccg atttgtttgc ccgagaaccc gacgcgccgc ttgccgaacg attgcgtccg
     1561 catacgcttg acgacgtggt ggggcaggaa cacctcatcg gcgaaggtaa acctttgcgc
     1621 gtggcggtag aaggcggcaa gccgcattct atgttgctgt gggggccgcc gggcgtgggc
     1681 aagacgacgt tggcgcagat tttggcgcag agtttcaacg cccagttttt gcctgtttcc
     1741 gccgtatttt ccggcgtgaa ggacatccgc gaggcaatcg ataaagccga aatcgctttg
     1801 cagcagggac gcgcgacgat tttgtttgtc gatgaagtcc accgcttcaa caaggcgcag
     1861 caggacgcgt ttttgccgca tgtcgaaagc ggtttgctaa cctttattgg tgcgacgacg
     1921 gaaaatccgt cgtttgaagt caatcccgcg ctgttgagcc gcgctcaggt gtatgttttg
     1981 caacccttgt cttcagacga cctgaaaaag ctgattgcca aggtattggc tttgcctgaa
     2041 taccaagagt ttacgattga aacggatgcg caaaaattac tcgtgaatac cgccgacggt
     2101 gatgcgcgca gattgttgaa tttgttggaa caacttttac gcgccgccga tacacgtcgt
     2161 ctgaaaaact taaccgccga atttctcgcc gacagtctcg gggcgcaaat ccgccgtttc
     2221 gacaaaggcg gcgagagttt ctacaaccaa atctccgccc tgcacaaatc cgtgcgcggt
     2281 tcgcatccga acgccgcgct gtattggttc tgccgtatgc tcgacggcgg caccgacccg
     2341 cgctacctcg cccgccgcat cgtgcgtatc gcttgggaag acatcgggct tgccgacccg
     2401 cgcgccttcc aaatcgccaa cgatgccgcc gccaccttcg aacgcttagg ctcgcccgaa
     2461 ggcgaactgg ctttggcgca agcggtattg tatcttgccg ccgccgcgaa atccaacgcg
     2521 ggctacaagg catacaacca aatgcgccgt ttcgtcaaag aaaacgccag cgacgaagta
     2581 cccgtccacc tgcgcaatgc cccgaccaag ctgatgaagg aattgggcta cggacgcgaa
     2641 taccgctacg cccacgacga accgaacgcc tacgccgccg gcgaaagcta tatgcccgac
     2701 ggcttggacg aaccggactt ctaccaaccc gtcccgcgcg ggttggaaat caaaatcggc
     2761 gaaaagctgg cgtggctcaa atcgctggat gaagaggcat taaaggcaaa atgaagcaat
     2821 gccgtctgaa gcagaacagt atggcaaagc cggtacaccg aaaaggcgta tcgggttttt
     2881 caggtgttat aatccgtttc aataaatttt gatgaatcat tacgtttatg tcccaaactc
     2941 acaacgaaaa ttcccgcgtc aaaatccccg ccgttttgca cctgatgcgc ttgggctacg
     3001 actacctctc gctcaaaaac gaaaattggg ataaaccaac caacatcttt cccaaaatct
     3061 ttatagaaag cctctgccgc atcaatcccg atttgtcgcc ggacgatgca cgccgtttgc
     3121 tgaccgacat acgcttagaa ctggacaacg aagacttggg gcaaaagttt tacgaacgcc
     3181 ttaccaatca atccggcggc aagaaactga tcgacttcca aaattttgat aacaacagct
     3241 tccacgttgt aaccgaattg ccctgtatca acggcgatga agaattccgc cccgacatta
     3301 ccctgctggt aaacggcatg cctttggttt ttatcgaagt caaaaagccc aacaacaaag
     3361 gcggaatcgg cgaagagcgg gagcgcatgg gcaaacgtgc gaaaaattcc aaatttcgcc
     3421 gtttcatcaa catcacccaa ttcatgattt tttccaacaa catggaatac gacgacggcg
     3481 caaccgaacc ggcacaaggc gcgttttacg cttcgtctgc ttacggtaag cccgtgttta
     3541 actacttccg cgaagaacat aaattaaatc ttgccgaatt attgaacacg ctttcagacg
     3601 gcctcgaaaa caacgtcctg caagacaaca acctgcccgt tatcaaacac agccccgaat
     3661 ttatcagcaa taaatcgccc gatacgccga ccaaccgcat cctgacttcg ctgctttgcc
     3721 gcgaacgtct ttcttttctt ctgcaacacg ggctgactta cgtcaaagca agtcaaggct
     3781 tggtgcaaaa acacatcatg cggtatccgc agctatttgc cactctcgcc attgaaaaac
     3841 atttggcaaa cggcggcaaa aaaggcgtga tttggcacac ccaaggctct ggcaaaaccg
     3901 cgcttgccta ttacaacacc cgctacctga cccactatta tgccaaacag ggcatcgtgc
     3961 cgaaattcta tttcatcgtg gacaggcttg atttattgaa acaggcgcag cgcgaattta
     4021 ctgcccgcga tttagtcgtc cataccatcg acagccgcga agcctttgct gccgacatca
     4081 aatccgccca aactttgcac aaccacgcag gcaaagcgga aatcactgtt gttaatatcc
     4141 aaaaattcca agacgacccc gatgtcgtcg ctcgtaacga ctacgacctc gccattcagc
     4201 gcgtctattt tctcgacgaa gtacaccgca gctacaaccc caaaggctcg tttcttgcca
     4261 acctcaacca gtccgacgta aacgccgtca aaatcgggct gactggcacg ccgcttatcg
     4321 gcgtaaccgc aggcaacgtc aacacccgcg aactcttcgg cgactacatc cacaaatact
     4381 attacaacgc ctccattgcc gacggctaca ccctgcgcct gatacgcgaa gaaatcggca
     4441 gccgatacaa ggcgcaatta caagaagcac tggcgcaact tgaaatcgaa aaaggcagct
     4501 ttgaccgcaa agaaatctac gcccatccgc acttcgtcca cccgatgctt gactacatct
     4561 tggacgactt cgccaaattc agaaaaacca accaagacga cagcctcggc gctatggttg
     4621 tttgcgacag cgcggaacag gcacgtcagc tttttgagca ttttcagata gcctcagacc
     4681 ataatttcac cgccgcgctg atattgcacg acgtcggcac taaggacgaa cgcgaccaat
     4741 gggttaaaga tttcaaagcg ggcaaaatcg acatcttgtt tgtgtacaac atgcttttga
     4801 ccggctttga cgcgccgcgc ctgaaaaagc tctatttggg cagactgata aaagcccata
     4861 accttttaca aaccctgacc cgcgtcaacc gcacctacaa atcttaccgc tacggctatg
     4921 ttgtcgattt tgccgacatc gagcgcgaat ttgacaaaac caaccgcgcc tattgggacg
     4981 aactttccaa cgaattgggc gacgaaatcg gcagctacag ccggcttttc aaaaccgccg
     5041 aagaaatcga acaggaaatt gcagacatta aaaacgccct gtttgatttc gataccgaaa
     5101 acgccgaaga attttgcagc caaattagcc aaatagcaga caaaaaacag ctgctcgccc
     5161 taaaaaaagc cctgcaaacc gccaaaaagc tgtacaacgt cctgcgcctg caaggcagcc
     5221 acgaatttct cgcccatctc gattttgaca agctcaatct tttgtaccgc gaaaccgccg
     5281 cgcgtttgga gaccttaaac cttgccgaaa aactacagca aggcgacacc gcccatttgc
     5341 tcaatgaagc gctggaagac gtttactttc aattcgttaa aatcggcgaa gccgaactca
     5401 aacttgccga cgatttgaaa gacatcatgc gcaaagtccg cgaagggctg gcgggcaact
     5461 tcgatcaaga agaccccgaa tacatcagcc tgcgcgaaga actggaacgc atcttcaaga
     5521 aaaagaacct caccgaagtc gggcaggaag aaatgcaggc aaacatcgct acgctcgata
     5581 ctgtctatgc ccaaatcaaa gagctgaacc gcaaaaacga tctcttgcgg cacaaatacg
     5641 gcggcgacac caaatacgcc cgcatccaca aacgcttgct ggaaaatccc gccctctacg
     5701 gcgacaaact taaagtcttc aacgcgctaa acggcgtcaa aaccgatgcc gaccaaaaag
     5761 tgctggatat ggagcagatt ttggataacc aaaattactt tgaaaaacaa atgcagggca
     5821 tcgtattaaa acgctttaga acggaacagc aatttcccgt tcaacccgca gacatccaag
     5881 ccatcaaccg cctgttggtg cgggaatatt taaaagaaag tgggcggatt tgaggagtag
     5941 attttcaggc tgccttaacc atttataaat acaggaaagc tatgtacaac aacgacatca
     6001 tcatctacac cacggaagac ggactatccg aatttaccct gcgcgagctt gacggcgaac
     6061 tatggcttac ccaaaaagaa attgccgaac tctaccaaac cagcaaacaa aacatcggta
     6121 aacacatcaa agccatattt gcagaacaag aactggacga ttcagttgta aactttcagt
     6181 ttacaactgc tgcagacggc aagaactacc gcatggggct ttatcccctc tccctaatca
     6241 tcgccgtcgg ctaccgcgtc cgctccacac gcggtacaca attccgccaa tgggcaacac
     6301 ggacattggg cgaatacatc agcaagggct tcgtattaaa cgacgaccgc ctgaaaaacc
     6361 cgcctgtcgg gacaaaccgt gccgaagatc agtttgaaca gttgctcaac cgcatcaggg
     6421 acatccgcag cagcgaacgg cggatgtatc tgcgtgtgcg cgaaatattc gccatggcgg
     6481 cggattacca gcccagcttc aaagaaacca cccatttttt caaaatcata caaaacaaac
     6541 ttcattacgc ctgtacccgt tataccgccc ctgaaattat ttaccaaagg gcagatgcgg
     6601 ggaaacccaa tatggggctg acccattggc ggggcaaaga aatcgccaaa gccgatgtaa
     6661 ccgttgccaa aaattacctg aacgaggcag aaatcgacgc cctcaaccgc atcgtttcca
     6721 tgtggctgga ttttgccgaa gaccaggcaa gcatgaaaaa acagatattc ctgcaagact
     6781 gggcggaaaa actggatgcc ttcctcgcct tcaatgaccg ccaagtctta caaacggcag
     6841 ggacaatcag caaaacgcag gcagacgaaa aagcccgcat cgaatacgaa aaatttgccg
     6901 ccgcacgcag actggaaaaa gaacgcgagg gcgaacgcca tattgccgaa ctgctgaacc
     6961 tgaaaaaata gcaggcatca atgcaggctg cttaaaaaag tgcggtcgga ttttgaaatg
     7021 ttttttcaga cggcctttga agagttttta gagatacagt taagatgaat agtgcattaa
     7081 agaatcatgc aacatatata aataacaaag ttgatacaaa tgaaatttcg cttagccaat
     7141 atatttcaac agacaatata ttgcaaaata agcaaggtat agaatgtgca acgtctttac
     7201 caattcaagg tggaaaagta acttcattta aaaagggcga tattttatta gccaatatac
     7261 gaccatatct aaaaaaaatt tggtatgccc aatttgatgg aggttgttct gctgatgttt
     7321 tagcgattcg tgctaataca gaaacagata gtcatttctt attttatgct ttgtttcgtg
     7381 atgatttttt tatccatgca atgaaaggtt caaagggcac aaagatgccg cgaggcgata
     7441 agacacagat tatggagttt aaaatccctg tttttgcccc tcaaacccag caatccatca
     7501 ccaccgtcct atccgctttg gacaaaaaaa tcgccctgaa caaacaaatc aacgcgcgct
     7561 tggaagagat ggcgaaaacc ctgtacgact actggtttgt acagttcgat tttcccgacg
     7621 ccaatggcaa gccctacaaa tcctcaggcg gcgaaatggt gtttgacgaa accttgaaac
     7681 gggaaattcc gaaggggtgg gaaagcgtag aattgcaatc ttgcttagca aaagtcccaa
     7741 gtactgtaaa aatatcaaat aaagacatta aggattttgg aaaatatcct gttattgacc
     7801 aaagtcaaga ttttatttgc gggtttactg acgatgaaaa atctatatta aaccctcaag
     7861 atgctcatat tatttttggt gatcatacaa gaatcgttaa gttagttaat tttaaatatg
     7921 cgcgaggggc ggatggtaca caagtaatat taagcaataa tgagcgaatg ccaaactatt
     7981 tgttttacca aatcataaat caaatagatt tatcaagtta tggatatgct agacatttta
     8041 aattcttaaa agagtttcca attattttgc cagataaaga tatttctcga aaatattatg
     8101 agatagtaaa ttatttcttt attaaaattc ggaataatat aaaacaaaac caccacctaa
     8161 cccaactacg cgatttccta ctgcccatgc tgatgaacgg gcaggtatcc gtggcggaat
     8221 aagccggttt caagaatccg agcgatacaa gaaaaagaat gagataaaaa atgactgaac
     8281 aacactttac cgaacaaacc aaagccctga ttgacagctt aaaaaccatt tgcgccaact
     8341 atggcttagg caatgacggc aacgagttca aaatcatcag tcaggcattt ttgtataaat
     8401 tcttaaacga taaatacgat tttgaagtta aaaaaattcg caaagaaaat cccgacgagg
     8461 aaattgagtt catcaatatg gatattgagg gaaaaacagc cgttttgaag cccgagcatt
     8521 ccataaaata tctgtccgag cggcaaaacg gtgcagattt tgccaaactc tttgacgata
     8581 cgctgaccga catcgccgcg cataatgcgg aattgttttc agtgaaaacc gaaggcgggg
     8641 cgaaaatcgt gttgttcgag cgtatcagtc aatatattac tgatgaaggt cgccgcgacg
     8701 atttttgccg cgcgctgatt tccaagctgg cgggttttag ctttgaggtg atttttgcgc
     8761 aaaaatttga ttttttcgcc acgatttttg aatacctgat taaagactac aacagcaatt
     8821 caggcggcaa atacgccgaa tactacacgc cgcacgcagt agcgcggatt atggcggata
     8881 ttttggtgcc ggaggaagtg cgcgggcaaa ttcgcagcgt ggacgtttac gacccgtcgg
     8941 ccggttcggg aacgctgttg atgaacgttg cccatgccat cggtgaagat aaatgtatga
     9001 tttatacgca ggatatttcg cagaaatcgt ccaatctttt acggctgaat ctgattttga
     9061 acaatttggt gcattcactg aataatgtgg ttcagggcaa tacgattttg tcgccgtatc
     9121 acaaagacgc ttcaggccgt ctgaaaaaat ttgatttcat tgtttctaat ccgccgttta
     9181 aattggattt cagcgatttc cgcgatcagc tggaaagcga tgaaaaccgc gaacgctttt
     9241 ttgcaggcat tccgaaaatc aaggcgaagg ataaggataa aatggaaatt tatcagcttt
     9301 ttatccagca tatcttgttt agcctgaaag aaaatggtaa agcggcgatt gtattgccga
     9361 ccggctttat cactgcccaa tcgggcatcg ataaaaaaat ccgcgaatat ttggtagaaa
     9421 acaaaatgct tgccggcgtg gtttccatgc cttccaatat ttttgccacc acgggaacca
     9481 acgtttccat cctgtttatc gataaagcca ataaagataa agtggtatta atcgacgcat
     9541 cgggcttggg cgaaaaaatc aaagacggca aaaaccaaaa aaccgtactt tctcgcgaag
     9601 aagaacaaaa aatctgcaat accttcacgc acaaacaggc agtggaagat ttcagtgtgg
     9661 taatcggcta cgatgaaatc aaagcgaaga atcacagctt gtcggcaggt caatattttg
     9721 aagtaaaaat tgattatgtg gatatttccg ccgaggaatt tgagcagaaa atggcgggat
     9781 tttcggcaga tttggacaag ctctttgcgg aatctgccga attggaaaaa gagattaagg
     9841 atagattagc aatgttgaaa ttcaattcat aaatactgac aaattaaact ggccaaactt
     9901 aaaaggccgt ctgaaaattt tcagacggcc ttttgtttca tttgttttga gaatcaatca
     9961 agcaagatta tttttcagct tcttcaaagc cgacgacttc gaggccgaag ccggtgaggc
    10021 cggtgaagga ggaaggctgg ccgaggacgc gcagtttttt gacgttgagg ccggcgagga
    10081 tttgtgcgcc tatgccgtag cttttgctgt cccatttgta ggcttggttt gcgcctttag
    10141 gcagggtgcg gtcgagcagg gatgcgccgt cttcggtgcg gtgcagaagg atgacgacgc
    10201 cgctttctgc ctgttggacg cgctcgagtg ctttgggcaa tgaccatgaa tggcgcgggt
    10261 tggcttgaat gaagtccatc acactgaagg gttcgtggac gcggacgagg gtttcggtgt
    10321 cggcggaggg cgtgcctttg acgagggcga ggtgggtttc gccggagagt ttgtcgacgt
    10381 aaacgtgttg ttggaactcg ccccacgggg tttgtacagg cgcattgccc atgtcttcaa
    10441 gcaggctttc ggtacggctg cggtattcga tgaggtcggc aatcgtgccg attttgagct
    10501 tgtgttcttc ggcgaatttc atcagttcgg gcatacgcgc catcgtgccg tcgtcgttga
    10561 tgatttcgca aataacggag gcaggaatca gcccgttcat ttgcgccagg tcgacgccgg
    10621 cttcggtgtg tccggcgcgg acgagtacgc cgcctttttg ggcgcgaagc ggaaagatat
    10681 gaccgggttg gacgatgtct tcgggtttag cggtcgggga aacggcggtt tgaatagtca
    10741 gggcgcggtc ggcggcggaa atgccggtgg taatgccgtg tgcggcttcg atggagacgg
    10801 taaagttggt gccgtattgc gcgccgtttt tttgggtcat catcggcagc ccgagttttt
    10861 cgaccatttc gccgtccatc ggcaggcaga ccaagccgcg cgcgtgtttg atcatgaagt
    10921 tgatggcttc gggcgtgacg aattgcgccg ccatcagcag gtcgccttcg ttttctcggt
    10981 cttcggcatc ggtgatgatg accattttgc cggctttgat gtcggctagg atttcgggga
    11041 tgggggagat atgggacatg gtgtgttttc ctgtgtgttc ggacgggcgg gcggtgtgtt
    11101 cggtatcgag ccaaaaggcg ggcatttttg ccgcctgttc gattttacgg gcttttttct
    11161 cgccgaagga tttgtttttc aaaagggcgg agagttcgcc tgtgttgagg gcaacggctt
    11221 cagcaaaacg agtttgcaaa ccgccgtagt atttttctat ccactgccgc aggttgtggc
    11281 ggcgcagacc ggctgtgtcg gtcatcgttt catcctttgt agaaatcgga tgtagcggat
    11341 tgtaatcgcg tatttaccaa aaagcaaatc tgtttttttc gccaaaccga attatttgct
    11401 ttttggtaaa cagatgccgt ctgaagccgg attcgtggct tcagacggca ttgccgcaat
    11461 ccccgccggc ggggcggctg tttcttcccg cccgcttccg attcggggtt tgccggaagc
    11521 ttattccgaa aaaaatttct gataaacggc cttgattgcc gggtacagca taggcggtac
    11581 gagcaggcgc aatatcggca atcctatgat gaatgcggtc ttcccgataa aaaccggacg
    11641 gattgccttc gcgccgaata tctttctgca tttttcttta aatgaaaaat atttccggta
    11701 gatgaggata atgtttttgg aaaaataaag ccttaccaat atagacctca gatagccgat
    11761 attgtcgaag acaaattttt gcaaaccgcc gtagatgggg gaatttttgt tttcgactaa
    11821 aaaatccaca ccccagtcga gatgcgtcag cacatcgctg aaatttttgt atttgatgtt
    11881 gtggctgata gagccgccga gaacgcggta ctggtaaaaa gggttatcgt aaaaggcaaa
    11941 agtcttgata aaacgcgcca attgcaaatt gtacgggaaa tcctcgtgaa tgtatccttt
    12001 tgggaaaaac agattgtttt tgatgattat ttcccgcctg acgatttttg tccacgcgtt
    12061 ggcgatatag taccgcccct ccaccagcgt ttcaaaatgg cggacaaaat cattatccga
    12121 aaagtccgcc cctttgggga tgacgtggta attgagggac gagggatgca ggatcaaatc
    12181 aacctttttg tctgcaagtt gttgtaaatc aaagagaatc cccccccccg cgttttttga
    12241 acggttggta tcggcccaat agtcgtcgct gtccaaaaag attaggtaat cgccttttgc
    12301 cgcccggata ccggcgttgc gggcatccga cagcccgccg ttttcttgat gaatcacttt
    12361 tatatgcgga tatttgcctg catattcgtc gcaaatcttc ccgcagccgt ccggcgaacc
    12421 gtcatcgacc aaaatcattt cataatcggc aaaattttcg gcaagcacgg aatccacgca
    12481 gcagcggagg tatttttcca cattgtaaat agggacgatg atggagaaaa tcataaatat
    12541 caatccatta tattaagatg tttgcgcgta tgcctcaaac ccgcacccgc aatgcgtttg
    12601 catccgcacc ctgtaacttt ataaaactta ttgctttatc aaggtatgga aacctgtttc
    12661 ccgaaaggcg gcgcaggatg cccgttccct gcatctttgc cttattccga catcagatgc
    12721 cccagcttgt ctgctttggt ttggaggtag cgtttgtttt caaggttttc cccgacgtgc
    12781 agggggatgc gttcgaccac gttaatcccc gcatctttca gggtttggat tttttcgggg
    12841 ttgttggtca gcagtttgac cgagcggatg cccagatatt cgtagataga ttgcgccaaa
    12901 cggaaatcgc gggcatcgac gggcagcccg agtgccaaat tggcttcaac cgtatccata
    12961 ccttggtctt gcagatgata ggcgcggatt ttgttaatca gcccgatgcc gcgtccttcc
    13021 tgacgcagat agacgatgat gccgcgcccc tctgtctgta cggccctcat ggccgcttca
    13081 agttgaggtc cgcagtcgca ttttctcgag aacagcgcat cgcccgtcag gcattcggag
    13141 tggatgcgcg tcagaaccgg attgccgtct gaaaaatcac cgacggtcag cgcgacgtgt
    13201 tcctgcccat ttgcctcttc aaagccgtgc atcgtaaata cgccccattc ggtcggcagg
    13261 cggcaggatg ccacatggtt caacatttca gacatcttca ctcccatctt ttccggcaga
    13321 catacccaac agcagtttca aaggttcgga caaagccaag gcaagagcca cccattccac
    13381 ctgtgctgcc gtacccgcac actctgcgca ttcaaattcc acatgaacca cgcccaacac
    13441 accgccactt tccgtgcaga ccggaatgga aatttgcgcc gccgaagcat gattgcgttc
    13501 tcccgaaagc tctcccaaat ccaaccaacg acgcacatcc gaggcaatat tcatccagcc
    13561 gctttgcgcc gaacggcaag ccagcgaaac cttgcctgcc gcttcatcca aatcccacag
    13621 gttttccaaa acctcgccct gccgggacag gcaaaccagc cgaaaggcgg cattttcaga
    13681 cggcatcagg gcataaacgg ctgcggcgcg cacgccttta cagcgtgaaa acaccgaatc
    13741 caaagccatg aaaagccgtt tcagaccttc ctcattcccg ctgttttgct ccaaataatc
    13801 ggcaagcctc caatcctcat gctcaagcca caaaaccgaa cgctcaatcg aagccgtccc
    13861 catatccatt accgtctgcg ccgtcagata ctccgcccga acctcgtcaa gcggcagctt
    13921 caaaccctga gtcagcagaa aatccttaat caaagcagca ggcataccca aattccgtca
    13981 ataaaaacaa aaaaccgccc gattccgatg tcaggcggac gaaaaacaga ttttaccgcc
    14041 ccaaacccca agccatcaac agccccgccg aaaaccttgc acaaaaaaac aaaatctgta
    14101 taatcgtcca attcccatgc ggacgtggcg aaattggtag acgcaccaga tttaggttct
    14161 ggcgccgaga ggtgtgagag ttcgagtctc tccgtccgca ccaataatga taaataaatt
    14221 atatagttaa tgcttatcgg gacaaattag tgccacaaaa cataagtcgt ctgcttttca
    14281 gcgggcggct tttttatctg cctatatgtt tcaacacgca gaacggtaca taaagtgccg
    14341 ccatttgttc cgcaccgaat cggaaacggg tgcttccgcc cgcaatcaga ttcaccgccc
    14401 ctaatgccgg ggtttcaact ggaaagaaga aaatattgat tttgatgatg gcatcaaaaa
    14461 accccttccg cccgtctgcc aaaaggaatg gttaagaaaa gcaatgcgtc ttttgatgga
    14521 acgcgatata taaggaattg aggggaaggg caggcgggtt tctgcccgtt aatcctgcgg
    14581 acgatcccgc caaacccgac acgcgcgggc atatggcgga ttgtttacaa tattgtctaa
    14641 aaatctgaac ctgcaaacgt gaaataaggt agaatacgcc ccgttatttt accgtccaat
    14701 ccccatttac caaggaaaaa tgatgagtac ttcattgagt taccgcgatg cgggcgtcga
    14761 tatcgacgca ggcgaccaac tggtcgaaaa catcaaaccg tttgccaaac gcaccatgcg
    14821 tccggaagta ttgggggatt tgggcggttt tggcgcattg gtcgaaatcg gcaagaaata
    14881 taaaaatccc gtattggtca gcggtacgga cggcgtgggt accaagctca aacttgcctt
    14941 tgattgggat aaacacgata cggtgggcat tgaccttgtt gcaatgagtg tcaacgatat
    15001 tttggttcaa ggagcagaac ctctgttttt cttggattat ttcgcctgcg gcaaattgga
    15061 tgttccgcgc gcaaccgatg tcatcaaagg cattgcacaa ggttgcgaag aatccggctg
    15121 cgccctgatt ggcggggaaa ctgccgaaat gccgggcatg tatcccgtcg gagaatacga
    15181 tttggcgggt tttgccgtcg gcgtagtgga aaaagagaat gtcattaccg gccgcagcat
    15241 cggcgcgggc gatgtggtat tgggtttggc ttccaacggc gcacattcaa acggctattc
    15301 ccttatccgt aaaatcatcg aacgcgacaa tcccgatttg gatgccgagt ttgataatgg
    15361 caaaaccttg cgtgaggctg ttattgcgcc gacccgtctg tatgtgaaac cgattcttgc
    15421 cgctttggaa aaatttacca ttaaaggtat ggcgcacatt accggcggcg gcattaccga
    15481 aaacgtgccg cgcgtgttgc ctgaaaacac ggttgcacaa atcgatgcta aatcgtggga
    15541 attgcccaag ctcttccaat ggcttcaaaa ggcgggcaat gtggaaaccc aagaaatgta
    15601 ccgaaccttt aactgcggca tcggcatggt cgttattgtt gctgccgaag atgccgatgc
    15661 ggttcagggt ctcttgggtg aacaaggtga aacagtttac cgtttaggtt tgatccgtga
    15721 gcgtcaggga gacgagcatc aaacccaggt tgcctgattg cttctatagc gaaatgccgt
    15781 ctcaaacatg ggtttggacg gcatttttat agtggattaa caaaaatcag gacaaggcga
    15841 cgaagccgca gacagtacag atagtacggc aaggcgaggc aacgccgtac tggtttttgt
    15901 taatccacta tatattgatt gatatgaggg tgggttacac atgcttaaac gatataagcc
    15961 attgtatatt tatgggattc ggaatggaac attttaccct gcattagtat ttccgctttt
    16021 tattatgccg tctgaaaata tcatgatgat acgttttcag acggcatagc tgatattgca
    16081 gtatcagata ggggcaaacg gaaaacacta aaccgaacaa tattactgct atgccaagcg
    16141 tacttatggg ggcataaact tacatgcttg gtctgttatc cgaatgaccg tacggggatt
    16201 aaaaccccta atatgccgtc tgaacccgcc ttattccagc acgggttggg ggacgttgtg
    16261 ccggatggcg taaacagcgg cctgcacccg gctgctgagg ttgagtttgc ggagcaggtt
    16321 ttgaacgtgg actttgacgg tggattcggc aagatcgagg tggcgggcga tgattttgtt
    16381 gctgtgtcct gcggcgagat agcccaagat ttccagttcg cgaggggtaa gtgaggagag
    16441 tgcctgtgtc ccttgggcag gttgggggga aatcaggctt ttaacgagtt tggcggtcat
    16501 ctcgggcgag aatacattat cgccttcggc ggctttgcgt atgctttcga gcagaaagtc
    16561 ggcgttgatg tttttcagca ggtagccgcg cgcgccgatg cgcatacatt cggtcaagtc
    16621 gtcgttgtct tcggaaacgg tcagcataat cactgcctgc tgcggattga tgctgatgat
    16681 ttgggagagt gcttcgcgtc cgttcatacc gggcatatca aggtcaagca ggacgacatc
    16741 gggttgcagc cggttgatca ttttaatgcc cgagaggccg tctgcggctt cgccgatgac
    16801 ctcaaaatca tgttggcgcg acaaaagtgc cttgatgccg ctgcggaaaa gggtatggtc
    16861 gtctatcagg ataattttaa tggtcatttc aagctttctt cagatacacc cgccaatgag
    16921 acggtggttc cctgttgggg ttgggaatag attttcaata cggcgcggat acgtttggca
    16981 cgctcctgca tgatgtgcag tccgacatgg ctgcccgtgg gttctcctat tttctccgtg
    17041 tcgaaacctt gtccgttgtc ttggatggtc atggtaaagc gtccgccgtg ttcggaaagg
    17101 gtgaatttta catgggtggc gcgggcgtgt ttgcggatgt tggacaggct ttcttgcagg
    17161 ataaaaatca tttggagctg ctcgtcctgt gtaggcaggt gcgtgccgtt ttcccaaacg
    17221 gtttcgacag tcgtgccggt ctgttgcgtg aagcgcgcga ataggtcggc aacggcttcg
    17281 ggaaattctt tattactgat tttggtacgg aagttgagca gcagttcgcg gacatcttca
    17341 taacattcct gcacgcctgt tttgatgaag ccgatgtttt ctgcggcttc ctcccgtttg
    17401 ttttcggcaa aggcggtttc cagcatctgt acctgtaggt ttaggaacgt taatgcttgt
    17461 gcgatgctgt catgtaatcc ttgcgcaatc aggttgcgtt cctgcaatac tgcaagcagg
    17521 cgtttttctt cctcctgttt tgcgccggca agcgatacgc ccaattgcct gcctagtgtt
    17581 tgaagcagga tgcggtcgtc ttcatcaaga gaaatgccgt ttggaaagct gagcaacagc
    17641 ctgcccaatg tttcgttctg gtactcaatg gggaagattt cctcatggta cttccccaaa
    17701 tccgaagctg ctgtgccgca atccgcatga tgaatggaaa cataaacatc ggatccgccg
    17761 tccaaacaaa ctctgccgga atctgctcct acggcgggca ggatacggtt tagaaaatgt
    17821 tctgcagcct gttgcggtat gtaggattgg tgcaggtccc gtgtagtttg gtacagcagg
    17881 gtcaggtttt gattttgttt ttcgagactg cgtgtctgct cggcgacttg tccttccaaa
    17941 tcatcatata aaattttcaa cctgccgccc atttgattga aacaacgccc gacctgtttg
    18001 aattccggcg taccgccttc gggaaccgga atatcgaaac acctccgtcc gatgcgttcc
    18061 gcaccttccc ttaacgcctg cagcggccgg ataacccaaa tctggtgcca aaacagcatc
    18121 agtacagacg acaccagcgt catcaacata attgcccatt gaaaacgcct gagccaccat
    18181 gtgttttttt cgttggcatt ttccaatgcc tgcaaaaaca gttcgatgtt tccggcaaag
    18241 cggtagagat cgacctgagt cggtcgccgg taggactgga gcggggggag gatgtgtgcc
    18301 tgccaatcta taatcagcat ggattgtatc aaatcataag caagaggggt gtccgaagga
    18361 atcagcggat ggatggcatc actttgtgaa atacgattca ggcttttttc aaattcgtca
    18421 acctgattgt ctatttggga gcggggagat ccttcacccg ccatgtatgc cagacggtat
    18481 gcctgcattc tcaagttgcc cgcctcttcg atgacggagg ccgcgttttc cagacgcaaa
    18541 gagagcagca gtgtcaaaac gacagacaat gccgccaacc cgacccacag tccggtcagg
    18601 agtttcaggc gtagggaaag gctgatgccg tctgaaaaac gggctggcag tatcatgctc
    18661 ggcgaaaaat tgttctaaat aatgcaaaca atatcattct ttgggattag atacaactgc
    18721 tcagaaagaa ttggttaagg aaaacttaat ccgcaccgct tcagtgttaa atcatgttat
    18781 gataaaattc agataatatg aatttatttg ccgtctgctt cagacggcat tttgagtcat
    18841 tgggaacggc agactgcaat catgaaaacc tcgaactttt ccaagagaac atccgcaatg
    18901 aaaacttttg ccctgatact ggccgacggt caggcgagcc gtatgggagg cgaggacaag
    18961 gggcttgctc ttttgggggg taaggcactg atagatcatg tcatcgacag ggtcaggccg
    19021 caggtcagcc atatcgccat cagcaccaac cggaatttgg aagaatatgc tcgaagaagt
    19081 ccgcatattt ttccggacgc acggcagtgg cagcatttcg gcccgctttc ggcattgtgt
    19141 accgcagcca acgatttgca gttggcgact gccgactggc ttttggttgt gccgtgcgat
    19201 atgccgtatc tgccgggcga tttggtggcg aggtttgaaa ccgtgtcgaa acgcacaccg
    19261 ttgtgcaatg cgtattatgt ggaaacgccg ataacgatgc actacaacat tatgtatatc
    19321 cgcccgcaga ttctgcaaag cgcgattccc tatctgtttt cgggtatgaa aacattaaga
    19381 agctggttgc agcagcaaag ggcgcggccg gtcagattcg agtttgacgg gcattttgcc
    19441 gacttgaaca cgcaaatcga tttgcaggag ggataagggc aagaccgccg accggcgtgg
    19501 aaaggaaagg tcaagccata ccgggcggtt tttgcccgaa tcggaggcat aatgctgtct
    19561 gaaggcattt cagacggcat ttttcgcggg gagatgctac aatttgcacc atttttaccg
    19621 acacagggaa acaggattat gtttacaggc attgttcaag gattgggaaa actgacggca
    19681 atccaccgcc cgtcggaggc atttcacact tatgtcgtcg agcttccgca agaggcggcg
    19741 gacaatctgc aacgcggcgc atcggtcgcc aataacggct gctgcctgac gattaccgaa
    19801 atcgaaggaa accgcgtcag tttcgattta atggcggaaa ctttggcaaa aaccaatttg
    19861 gggctgctga aggaaggcga ttgcgtcaac atcgaacggg cggcgcgttt cggagacgaa
    19921 atcggcggac acgtcatgag cggacacatt atggcaaccg tgcctattgt cgaaatcgaa
    19981 cgggacgggt tcaaccgcac ggtttggttt tcgctcccgc atgaactcaa accctatatc
    20041 ctgaccaaag ggttcgtcgg cttggacggt tgcagcctga ccataggcaa agtcgaagac
    20101 ggccgtttca atgtccatct gattcccgaa actttggaac ggacgctgtt cggcagcaga
    20161 aaggtcggcg acaggatcaa catcgaaatc gatccgaata cgcaggcaat cgtcgatacc
    20221 gtcgaacggc tgatggcgca aagatatgca aagtgaggca gagatggaat tgaacgaatt
    20281 tctcgataaa gcctatgccg ttttgcggcg tttggatgcc gtgcttccgc ccgaacccgg
    20341 gcatacggat tggaacgcgc ttgccttccg ctggcagagt gcgggcaaaa aaggtttttt
    20401 ggaacacttg cccgatccgc acacctttcc cttggtaagg ctggcgggag tcggcaggca
    20461 aaccgaattg ctggtgcgta ataccgaaca gttcattgtc ggcagacccg cgaacaatgt
    20521 attgatgagc ggcgcgcgcg gaacaggcaa atcctcgctg gtcaaagccc tgctacacga
    20581 atatgcggat aagggattgc gcctgatcga agtcgataaa agcgatttaa tcggcctgcc
    20641 ttacctgttg acgcttttga aggaacgtcc ggaaaaattt atcgtatttt gcgacgattt
    20701 gtcgtttgaa agcggcgatg aaacctataa ggcattgaaa accgcgttag acggcggtct
    20761 gtctcaacgt tgcgccaacg ttttggttta cgcgacttcc aacagacggc acctgatgcc
    20821 cgaatatttt gacgagaatg ccggtacgac ggggatgcgg ggggaaatcc atcagaaaga
    20881 agcggtggaa gaaaaaatat ccctgtccga ccgattcgga ttgtggctca gtttttatcc
    20941 gttcgatcaa aacgattatc tggcggcggt gcaaaattgg ctggaagatt tcggcgtgcc
    21001 gtatgatgaa accgcacaga tggcggcatt gcagtgggcg cagacgcggg gcagccgttc
    21061 gggacgttcc gcgtggcagt ttgcctgcga ctgggcgggc aggctgccga aacaacgggc
    21121 gttgtgagcg tttcggggat tatttgaggc aggaagaaag ccacatttgc tgctgttgcg
    21181 gtgtgaggat atggaagaac cggtgttgga tttccaattc gtccaccgca aaatccatac
    21241 cggacaaata gcggctttcg acataatcgc gcgcctcgtt ccgattaaaa acatccgagg
    21301 aaatgatttc gacgacagac cggcggcggc tgtgttcgga atgcataacc ttcaaacgcg
    21361 ccctgtcgcc cgccattttg aaggcggtgc ggattttacg cagctcattg tgctgacttt
    21421 gggtcagccc gagtcggcgt atgtcgcagt tcggttgaaa atcgttcggg gggatataag
    21481 ccgtggcggc gtgggaaagc tgacaggaaa gcaaagccat acttaaaaaa gaggcggcag
    21541 gtttggcaaa acggcaggga gcaggcagtg gcacggcttt tcctttaatc cagatatttc
    21601 ggacaatata cttgattgcc gtgtgaaaga tggtattatg gcagttaact tttgttaata
    21661 atgctgtttc ggggctttcc cgcgttgagt ccgcccgtaa aggcattgaa ggaaaaaata
    21721 tgaccgccta ccgtatcgca cccagtattt tgtcggcaga ctttgcccgc ctcggagaag
    21781 aggtggaaag cgtcattgcg gcaggtgcag atctgatcca ttttgacgtg atggacaacc
    21841 attatgtgcc gaacctgacc ttcggcccta tggtttgcgc ggcgttgaag ccttatgcaa
    21901 gcgtgccgat cgatgtgcat ttgatggtcg aacccgttga cgacctgatt cagtcgtttg
    21961 ccaaagcagg agcatcgatt atcacttttc atcccgaagc gagccgccat atcgaccgca
    22021 gcttgagcct gattcgtgat atgggctgtc aggcggggct ggtgttgaat ccggcaacgc
    22081 ccgtatatct gttggaaaac gtattggaca ggctggatat ggttttgctg atgtcggtca
    22141 accccggatt cggcggacaa agcttcatcc cgcacaccct tgaaaaaatc cgccgggtgc
    22201 gggcgatgct ggatcggtac gaagcacaaa gcgggcggca catcgccatc gaagtggacg
    22261 gcggcatcaa aaccgacaat attgccgccg ttgcccgagc tggtgcggat acctttgttg
    22321 ccggttctgc gattttcggc aaaccagact ataaggcggt gattgatgcg atgagggcgg
    22381 aactggaaaa aagcggcagg ctgaaccttg cgccaatgat tgaaatagaa atgccgtctg
    22441 aaaattagct ctgtttcaga cggcattgcg ttatttttcg acgggcagcc cgaaatgcag
    22501 gtaggcgcgt tcggtcgcca tcctgccgcg cggggtgcgt tgcaggaagc cttgttggat
    22561 aaggtagggt tcgataacgt cttcgatggt gtctgtagat tcgccgatgg cggcggcaac
    22621 attgtccaaa ccgaccgggc cgccgccgaa tttgtgcaaa acggcttcga gaaatttcct
    22681 gtccatcacg tccagcccct gcgcgtccac gtccagcata cttaaagcgg catcggcgat
    22741 gccgccgtcg attgtgccgt tgtttttcac gtcggcgaaa tcgcgcacgc gtcgcaacag
    22801 gcggttggcg atgcgcggcg taccgcggct gcgtttggcg atttcttccg cgccttcttc
    22861 ggacatatcg agctgcaaca gttgtgccga acggctgacg atggtggtaa ggtctcggtt
    22921 ttcgtaaaac tcaaggcggg agacgatgcc gaagcggtcg cgcaacggat tggtcagcat
    22981 accggcgcgg gtggtcgcgc cgatgagcgt gaagggcggc aggtcgattt tgacggaacg
    23041 ggcggcgggt ccttcgccta tcataatgtc gagccggtag tcttcgagcg cgggatagag
    23101 gatttcttcg acaacagggc tgaggcggtg gatttcgtcg atgaacaata catcgtgcgg
    23161 atcaaggttg gtcaaaaggg cggcgaggtc gcctgcgcgt tcgaggacgg ggccgctggt
    23221 ttggcgcaaa tttacgccca attctttggc gatgatgtgc gccagtgtgg ttttgcccag
    23281 tccgggcggg ccgaagagca aaacgtggtc gagtgcttcg ccgcgttttt tggcggcttg
    23341 gatgaaaatg gcaagctgtt ctttggcttt gtcttgcccg atgtagtcgt ccagcgtttt
    23401 ggggcggagg gcgcgttcga gcagttcttc ctgtgcggag gcggtttggg cggcaacgat
    23461 gcgttgcggt tgcgcggcgg tcaggttgtc ggtttgcagc atagtgttcc ctttgtcggg
    23521 tatgccgtcc gaacggtcgg cggcgtttca gacggcattg aaaagataac ggtcaaaagc
    23581 gttttatccg cgggtttcgc gccaaacgag atagtcgcgc aggggcggca taggcgggtt
    23641 gatgcagtgg gggcagatgc cggtggtgtc ttcgtcgtcg ctgtcgatgt ggtaggcgtt
    23701 ggggtcgaag cggatttgct gcatgacggc ttgcgccaat gcctgcgcct gaacaacgtc
    23761 gaaattgaat ttttcgagga tttcgttcaa caggcggatt tcgccaccgc taaattccgc
    23821 actgtgtttg aggatgaggc gttgataatc ttcgttgttg attttaggca ataaatcggt
    23881 tttcagggtc atgattttat gtcgggggca ggatggctga agcgtgaatt atagcggata
    23941 tggcagcggc tgttgcaaag cttggattgg gtatcgtcct caaaacttta tagtggatta
    24001 acaaaaacca gtacagcgtt gcctcgcctt gccgtactat ctgtactgtc tgcggcttcg
    24061 tcgccttgtc ctgatttttg ttaatccact ataaaagtgg ctgcattgga cgttttaatg
    24121 tttttctgtt ttgtcttgcg ccgctcgaaa cgttttttgc aatggggcga tttgtgccag
    24181 ccgcgcccgg tcgatttgtt cggcgataag gtgcgctttt ccctgcgcgc ggcattcggc
    24241 ggcgattttg cccgaatccg cctgattggc ggcttcgagc agggcgaacc agtgtgcgcg
    24301 ttgcgggtag ggcgtgtgtt cgcggttcag acggccttgc gtgtcggcaa tgcagacgtt
    24361 caatgccgtc tgaaagcgtt cgggacggcg gaaggcatcg gtttttttca atacgttcag
    24421 aatggtttgg cttttaagct gtccgacttg gtggaaaata atgtgccaac ggcaaaccaa
    24481 ttcggcaagc tcggcgcaat gtttcggcgc acgcagccgc tgattgactt cgcgcacggg
    24541 ttcgacaccg gcgaggtcgt gtccgtggtg gcgcggcagg atgtcggacg gtgttttggc
    24601 tttgcccaag tcgtgcagca gggcggcata gcgttcgggc aggctcaaac ccatatcggc
    24661 ggcgcgttgc agcgtcatca gggtatggat gccgctgtcg atttcgggat ggtagtcggc
    24721 gcgttgcggc acgccgaaga gggcattgac ttcgggcagc aagactttga gcgcgccgca
    24781 ttcgcgcaac acttcaatca ttttgcgcgg atttttttcc atcaaacctt tcgcaaactc
    24841 ctgccagacg cgttcggcaa ccaatgcgtc cgcttcgccg ttttccacca tctgccgcat
    24901 cagctttatg gtttcttcgg cgatttcaaa cctgtaacgc gcggcaaagc gggcagtacg
    24961 caggatgcgg acggggtctt cggcgaaggc cggggaaacg tggcgcaaaa tgcctgccgc
    25021 caaatcccgt tgtccgccga aagggtcgat aatcttgccg tccgcatctt gcgccatcgc
    25081 gttgatggtc aggtcgcggc gcatcaagtc ctgctccagc gtaacgtctt tgtcggcgtg
    25141 gaaactgaaa ccgacgtaac ctttggcggt tttgcgctcg gtgcgggcga gggcgtattc
    25201 ttcgtgcgtt tcgggatgga gaaacacggg aaaatctttg ccgaccggct ggaagccttg
    25261 cgccagcatg gtttgtgcgt ctgcgccgac gaccacccaa tcgcggtctt tgacgggcaa
    25321 gcccaaaaga taatcgcgga cggcgccgcc gacgaggtaa gtctgcatat gcttcctatt
    25381 ttaaagttat caacaatgcc gtctgaagcg gcttcagacg gcattgttcc aaccggcggt
    25441 tatgctacgc cttttttctc caagtaactt tcgtaatcgc ccaagtagtg ttcatatccg
    25501 cctttgccgt ccagttcgat gatttgggtt gccaaggagg aaacgaactg acggtcgtgg
    25561 gagacaaaaa tcagcgtgcc gttgtatttt tccagtgcca tgttcaagga ttcgatgctt
    25621 tccatgtcca tatggttggt cggttcgtcc atgactaaga cattgggttt caacagcaac
    25681 agtttgccgt aaagcatacg gcctttttca ccaccggaga gaaccttcac ttttttcacg
    25741 acatcgttac tgccgaagag caaacgcccc aaagtgccgc ggatgacttg ttcgtcgtcg
    25801 ccttcctgcc cccattggcg catccattcg ctcaggtcca tatcgacgtc gaagtcgttt
    25861 tcatggtctt gcggatagta gccgacactg gctttttccg cccatttgat ggtgccttcg
    25921 tccggtaaca ggccgtctga atattcgggg ttgtacgcgc ctgccaagag tttcagcaag
    25981 gtagatttac ctgcgccgtt cgggccgatg atggcaaggc gttgtcccgc ttcgaggatg
    26041 aagttcaggt ttttaaacaa ctgggtttca aagcgtttcg ccagtttttc cacttccaca
    26101 gcctgacggt gcaacttggc tttttcgtcg gcttcaaaac ggatatacgg gttttggcgg
    26161 gtggaaggtt tgacttcgac catctccgat ttgattttgt ctgcctgttt cagacggctg
    26221 gtcgcctgac gggctttgga tttgttggca ctgaaccgcg ccacaaattc ttgcaattcc
    26281 tgcaattttt ctttcgcctt ggcattgtct ttcagggcgc gctcacgcga ttgggcagaa
    26341 gcgagcatgt aatcgtcgta gttgcccgga tagatggtga tagtgttgta gtccaaatcc
    26401 gccatatggg tgcagacttc gttcaaaaag tggcggtcgt gcgaaataat aatcatcgta
    26461 gaatcatatt ggttcaacac gccttccaac cagcggatag tgttgatgtc caagttgttg
    26521 gtcggttcgt caagcagcag cacatccggc ttggagaaca gggcttgcgc cagcaataca
    26581 cgcagtttga agcccggggc aacttctgcc atggtcgcat tgtgcaaatc ttcggaaatg
    26641 cccacgccgc tcaacaattc ggcagcgcgt gcttcggcgg tgtagccgtc gtattcggcg
    26701 aacttggctt ccagttcggc ggctttcatg taatcgtctt cggtggcttc gaggttggcg
    26761 taaatcgcat cgcgttcggt catcgccgcc cacatttcgg tatgccccat catcaccacg
    26821 tccagcacgc gcatctcttc gtaggcaaac tggtcttggc gcaatttacc caaacgcacg
    26881 ccgttttcaa tcgcgacttc gccggcagtc tgttccaaat cgccgccgag gattttcatg
    26941 aaggtggact tgcctgagcc gttggcgccg atcaagccgt agcggttgcc ttcgccgaat
    27001 ttgacggata cgttttcaaa cagcggcttc gcgccgaact gcatggtaat gccgttggta
    27061 gaaatcatga ttggtaaagc ctttattgaa caagagatta aatttttcga attttcggat
    27121 tgtaacataa ccgccccgcc gtctgaaaca gacgggcaaa ttttcagaca cttatagtaa
    27181 aatatccgtt ttattatttc agacaaaagg tttccaaatg atagtccttc acggcatccc
    27241 aaattgcgat acggtcaaaa aagccaaaaa caggcttgcc gaatatggta tggaatttga
    27301 atttcgggat tttaaaaaac aggtgccttc cgaagcggaa atctgctcgt ggctggaaca
    27361 agtgcctttg gcaaccctgc tcaacaaacg cgggacaagc tggcgcaaac tcgatgccga
    27421 aacacagcaa aaagtgctgt cctcgacggc ggaggccgtc aaactgatgt ccgaaatgcc
    27481 gagcctgatc aagcgtcccg tattggagtg cggcggcaag gtttacgccg gcttcagcga
    27541 agaaacctac gacggcatct tcaaccggca agccccgtgc agacagggat aaaaaccgtt
    27601 acaatacccg acttgaattt cccgttccca ttctatatcc cgatttaaaa tatgttccat
    27661 tccatcgaaa aatacagaac gcccgcccaa gtccttttgg gcctgattgc attaaccttc
    27721 gtcggcttcg gggtcagcac ggtatcccat ccgggtgccg actacatcgt ccaagtgggc
    27781 gacgaaaaaa tcagcgacca ctccatcaac aacgccatac agaacgaaca ggcggacggc
    27841 ggcggtcctt cgcgcgacgc ggtgttccaa tccctgctgc aacgcgccta cctgaaacag
    27901 ggctcgaagc tgatgggcat ttcggtttct tccgaacaaa tcaagcagat tatcgtagag
    27961 gatcctaatt ttcacgatgc gggcggcaaa ttcgaccacg cgcttttaaa ccgctacctg
    28021 tcgcaacgcc atatgtctga agaccagttt gtcgaggaaa tccgcgatca gtttgcctta
    28081 cagaatttgg taaacctcgt ccaaaacggc gtattggtcg gcgacgcgca ggcggaacag
    28141 ctgatcaggc tgacccaggt caaccgcacc atccgttcgc acactttcaa ccccgacgaa
    28201 ttcatcgccc aagtcaaagt gtctgaagcc gatttgcaga aattttataa tgcgaacaaa
    28261 aaagactacc tgcttcccca agcggtcaaa ttggaatatg tcgcattgaa tctgaaagac
    28321 tttgcagaca aacaggccgt cagcgaaaca gaagtgaaaa atgcgtttga agagcgcgtg
    28381 gcgcgtttgc cggcaaatga aaccaaacct tctttcgagc aggaaaaagc cgccgtcgaa
    28441 aacgaattga aaatgaaaaa ggcggttgcc gacttcaata aggcaaaaga aaagctgggc
    28501 gatgacgcgt tcaaccatcc ttcctcgctt gccgaagccg ccaaaaacag cggtttgaaa
    28561 gtcgaaaccc aagaaacttg gctgagcagg caggatgcgc aaatgtccgg tatgcccgaa
    28621 aacctgatca atgccgtatt cagcgacgac gtattgaaga aaaaacacaa ttccgaagtg
    28681 ctgaccatca acagcgaaac cgcgtgggtc gtccgtgcca aagaagtccg cgaagagaaa
    28741 accctgccgt ttgccgaagc caaagatgcg gtgcgtcagg cttatatccg taccgaagcc
    28801 gccaaacttg ccgaaaacaa ggcaaaagaa gtgctggcgc agttgagcgg aggcaaggca
    28861 gttgacgtga aatggtcgga agtgtccgtt ttgggcgcgc agcaggcaag gcagtccatg
    28921 ccgcccgagg cttatgcgga actgctgaaa gcaaaaccgg caaacggcaa acccgcctac
    28981 gtcaggctga ccggtctgcc ggcacccgtg attgtcgaag tacaggctat aaccccgccg
    29041 gatgatatcg ccgcacagct tccgcttgca aaacaggctt tggcgcaaca gcagtctgcc
    29101 aatactttcg acttgttgat acgttatttc aacggcaaaa tcaaacagac caaaggagcg
    29161 caatcggtcg acaacggcga cggtcagtaa ttgacacttt tgttgacaaa ataacggtcg
    29221 gaatattccg gacgttttcc catacggcag aatatgatga gccacaaaaa acaagatgcc
    29281 ttccaaggat tgatcgacgc gctgaaggtt ttacccaacg tcgggccgaa attggcacag
    29341 cggatagcgt atcatttgct ccaacacaag cgcaaagagg ctgaaaaact ggtggatgcc
    29401 ttgcagacgg cattgaagca ggtttaccat tgcgcgatgt gcaacacgtt ttgcgaaggc
    29461 ggattgtgcg atatttgtgc cgatgaaaca cgcgacgggc ggcggctgat ggtggtgcat
    29521 atgcctgccg acgtgtcgaa tatggaagcg gcaaactgcc acgacgggct gtatttcgtc
    29581 ctgatggggc aaatcaatac ggcactggga atggacgtat ccgccatcgc attggacagg
    29641 ctggcgcaac ggctgggcgg cggggaagtc gaagaaatca ttattgcaac cgcttttacc
    29701 gcagaaggca atgcgacggc gtatgtcctg tccgagtttt ttaaaaacct gccttacaaa
    29761 gtcagcaggc tgtcgcaggg cattcccttg ggcggcgaat tggaatatgt cgatgcggga
    29821 acgctggcgc aggcggtgta cgaacgccgc ctgattaaag aaggcggggc ataacgccgc
    29881 caatgcaaaa tgccgtctga agccttcaga cggcattttt ccgggcggtt tacttgcagt
    29941 ttagcccttg aatctgcaat gcgcctccgt tgccgacttt gaacacgcac tgcgtcgcgc
    30001 tgctgccgga cacggtaatg ctgccgctgc cgccgttcag gctgataggt tccttaacac
    30061 ccataccgac tgccgttgcc gccagctttt gcgcgtcggc ttcggaataa ggcgtgttgt
    30121 tggcatcgat tttgatttct ttggcgtagg cagccgcagc ggcggataag gcgatggcgc
    30181 acaaagcaaa agcagttcgg atattcattg ttttcctcaa gaaacggttt ttctgaagga
    30241 aaggataggg cagtttgacg cgttgtgcaa gttttccgcc cgctctgccg ccccgtcccc
    30301 cgcactcggg cgttcggacg ctgcctgggt tttacggtac aataaacttt tgccgccgcc
    30361 cccaaaccgc cgtccggggc ggcacgtttg cagacatttt taaggtagcg ttatgttttc
    30421 tctagaggct tggataggct tgaggtatct cagggcgaaa aagcgcaacg gctttatgtc
    30481 gtttatcacg atggtttcga ttgccggaat cgccttgggc gtaaccgcgc tgattgtcgt
    30541 cttgtcggtt atgaacggct ttcagaaaga aatacgcggg cagctcctga atgtcgcgcc
    30601 gcacgccgaa atcggctata tcgacaatac ggatacggat tggcgcaacc tgcttcgatt
    30661 taccgaaaac cgcaaaggta ttttggctgc cgcgccctat gtttccaatc aggcattgct
    30721 ggccaatgcg ggcgaaatca ggggcgtgca gatgcgcggc attttgccgt ctgaagagcg
    30781 caaagtggtg gaatacggcg acaaaatgcc ggcaggcaaa tttgaagacc tgattccggg
    30841 cgagtttgac attatcctcg gtgtcggctt ggcggaggct ttgggggcgg aagtcggcaa
    30901 taaagttacc gtcatcacgc cggagggcaa tgttacgccc gccggagtcg taccgaggtt
    30961 gaaacagttt accgtggtcg gtctggttaa gacgggcgtt tacgaagtgg acaactcatt
    31021 ggcgatgacg catatccaag acgcgcgcgt actgtaccgc ttggataagg aagttgcggg
    31081 gctgcggctg aaactcgccg acccgcaaaa cgcccccgcc ttgacggcaa cactgattcc
    31141 ggaggcgcaa agggacgcgg tttgggtgcg cgattggacg tacaacaacc gcagctattt
    31201 tgaggcggtc gagttggaaa aacggatgat gttcatcatc ctgacgctga ttatcgccgt
    31261 ggcggcgttc aaccttgtct cttccttggt gatggcggtt acggaaaagc aggcggacat
    31321 tgcgatttta cggactttgg gtctttcccc tggcggcgtg atgaagattt ttatggtgca
    31381 gggcgcgttt tcaggctttt tcggcacgct ggcgggtgtg gtctgcggcg tgcttttggg
    31441 ttggaacgtc ggcagggtcg tggcgttttt tgaaaacctg ctcggtgtcc acctcatcaa
    31501 ttcgcaggtt tattttatcg actacctgcc cagcgatgtc gatatgggcg acgttgccct
    31561 gattgcctgc atttctttgg gactgtcttt cgttgccacg ctctacccga gccggcgcgc
    31621 gtcaaaaacc caaccggcgg aggctttgcg ttatgagtga gttgattttg aaatgcgaag
    31681 gcgtgggcaa acgctatcgg gacggcggtt tggacgttcg ggtgctgcac ggcttggatt
    31741 tggaaatccg cgcgggggaa agcactggca tcatcggttc ttcgggcagc ggaaaatcga
    31801 cgctgctgca tattctgggc gggctggata tgccgtctga aggcagggtg ctgctgatgg
    31861 gcgaggattt gcgtacctta aaccagcggc gtttgggcga tttacgtaac cgccatctcg
    31921 gtttcgtgta ccagttccac catcttctgc ctgaattttc ggcactggaa aacgtgatga
    31981 tgccgcttct gatcggcaaa aaaagccgtg aagaggctgc ggaggcggca atggcgatgc
    32041 tggaaaaggt cggactgaaa caccgttcga cgcaccgcgc gggcgaactg tccggcggtg
    32101 agcggcagcg tgccgccatt gcgcgcgcct tggttacgca acccaaatgc ctgcttgccg
    32161 acgaaccgac cggcaatctc gaccgagcaa acgcaaggaa tgttttggat atgatgctgg
    32221 aactgaaaac agaattggga acggggctgg tggtcgtaac gcacgacgac gaactcgccg
    32281 gccgcttcgg gcgcgtgatg gtcatgaaag acggcagcct gcaccccaaa cagggcgcaa
    32341 acgcctaaac agccgaaaca acaccgcgcc gtctgaagcc ccacgccttc agacggcatt
    32401 tcgacaagga atacgatgga acggcagaca gatgaatttg cccaagcggc ggcaagggcg
    32461 gcaatccgtt ttttggaaca ctatgcgggt tctggcgacg aagtgcttgc aaactgtacc
    32521 gaacgcctgt ttcaggcatt gcagaacggt cattcgttta tccgtttgag cggtggcgag
    32581 gctgatgcgc tgtcggcact cgcacccgtg gtcggaacat ccgccgcgcc tttgattttg
    32641 gaaggcagaa ggctgttttt gggcaggatg tggcagttgg aatacgattt ggctgccgag
    32701 ataaaacgct tggcggcagc cggcacatcc gcccccgacg cgacaggtgc gcggcaaaac
    32761 ctcgcaaaat ggtttcaagg cgcgggcagc gaaggacagc gcgatgccgc cgccttggca
    32821 ctgttacagt cttttatggt gattaccggc gggccgggaa cgggcaaaac gacaacggtt
    32881 gccaaactgc tggcgctgat ttgcggtgaa gacgaaaatc ttccccatat cgcgcttgcc
    32941 gcaccgacgg gcaaagcggc agcacatatg gcgcgcgcgc tgcaccgtgc aatcaacggt
    33001 tttgacgcgc cggaggccgt ccgccgccat ttgctcaaac tggaagggca aaccgtccac
    33061 cgattactga agctgcgccc gcccaaaatg caggcggcgt tcgaccatat ccgtccgctg
    33121 ccgttcgatg tgctgattgt cgatgaagcc tccatgttgg ataccgcatt gatgctgcaa
    33181 cttttgaaag cggtcaaaac cggcgcgcgc gtgattctgc tgggggatga aaaccagctc
    33241 ccgtccgtcg gaatcggggc ggtgctgtcc gttttgtcgc aaaaaaccgt tttggacgga
    33301 gaaacgcacc aaaggctggc cggcttcctt ccggaacacg gtttcagcgt cagcgcaaat
    33361 ccgcccgtgt tggcgcaaaa caccgcccat ctgtcgttca gccaccgctt cggcgacaac
    33421 agcggcatcg gctgccttgc ccgtgccgcc gtatcgggcg atgagggggc gtgggcattg
    33481 tttgaccggt ttccggacga actggaacat tcggaatgca gtccgaacgc tcgagtcgaa
    33541 aggttgtacc gggcgcacaa agcctattgg caggcggtaa aagacggcaa tatcgaagcc
    33601 gcatacgcag gcatctcgga tatcgtggtc ctggcagctt ggcggcagga tgcggaagac
    33661 ttcaacgaag cctattgccg ccatgtacgc cgcaaaatga acataccgga acatcaggcc
    33721 tatttcgccg gagaaccgat tatgatcaga cagaacgact acgcgcttga actgttcaac
    33781 ggcgacatcg gattgattat ggaagatgtc ggacggcagg gcagccttgc cgcctatttt
    33841 gccgatgcgg acggatttaa aaaggtagcg gtaagctgcc tgcccgattt cgaacccgca
    33901 ttcgccatga ccgtccacaa aagccaaggt tcggaatatc gggaagtctg gctgctgccg
    33961 ccttccgccg caccttcgga cgaaggggac gacgcgcttt cgggcttgag cagagggctg
    34021 ctttacacag ccattacccg cgcgagagag aagttcgtat tcttcggcgg ggaagaagcc
    34081 ttccggcaag ctgccgtcac cgtcaaaacg cgtcagacgg cgttgggcag tatgctcgag
    34141 cgggtatttt cacaagaata atccgcccga atgccgcgcc gccgcccctt atgccttttt
    34201 caaacggtat aggaaagtgg tttcccgggt tcgcgcaaaa gcaagcggat cgctcggatt
    34261 cgcggctttt ttgtgcttcg gcttggtttt tatcatatcg gcaacacgca aacccgcccg
    34321 gataaatgcc ttttccaaaa aatcggcagg gcgcagcccc aaatgctctg caaggtcgga
    34381 aataatcagg cggatttctc cgtcgggatt cagatgtttc ggcgcatccc gcaaaaacgc
    34441 agccagcatc gcagattcgg ggtcgtataa cgcggattcg acggcggaag tcggcttggc
    34501 gggaagccag ggcggattgc agacgatcag atcgacaaac ccttcgggaa acagatcggt
    34561 ttcgcggatt tcaacctgtt tttcaaagcc caaacgggca atattggcac gggcgcaggc
    34621 gacggctttc ggattggtat ccgtgccgat gacggaagga atgccctgtt tcgccaaaat
    34681 ggcggcaagc acgcccgagc ctgtcccgat atcgaatgcc gtctgaaaac ccgttgacgg
    34741 cgcatgggcg agcaggtcga ggtattcgcc gcgcaacggc gagaatacgc cgaaaggaac
    34801 gtgtatgctg ccgcccagct gcggaacggc aacccctttc ttatgccact cgtgcgcacc
    34861 cataaacccc agcagcagat tgagcggcag gaaaaacggt ttgccgtccg cctctccgta
    34921 cacgtcgagc agggcggcgc gtatatcggg cgcgcgtttg ttgtccaaca caaaaccggg
    34981 gcggatttca acggcaagca tattcagaat acggctctgc tgcgcctgct tcatacggtg
    35041 ggcatggaaa agggcggcga tatccgcatc ggaacggacg gcggcaggtt tgcgaaccct
    35101 cttcttcatt gcggaaagca cctgtttggc attgtggaaa tcgccctgcc agacagttgc
    35161 aatattttga taggcagcct tcaaaatgcc gtttgcacca ctttcgcgga cataatgcca
    35221 acctttgggc ggcttttgca gactttcgtt gcgccattcg aacccgttat cgggaaaaat
    35281 aaaagaagac atggaatacc tgcgtcatgt tttgaaaata gggcggcaga accgcaaacc
    35341 atacggatgg tacagcaagg agcggcaaca cagaacagtt ttttgttccc gccttgtctt
    35401 tccaagccca tgccgtctga agccggaatg tttcagacgg catcgcatca aactccataa
    35461 ataaaccaca tatgcttgaa ataatacctt caaccccaat gtacgcgaaa atcggcaatc
    35521 tgtcagacac aagagagtac ctatgacaca aaaagaaaag cattttgagg aatatgccgc
    35581 cttggcaacc cttcctttgc gggatgtcgt cgtttacccg catatggttc tgccgctgtt
    35641 tgtcggcagg ccgaaatcca tcgccgcact ggaaaacgcc attacccgcg aggagccggt
    35701 tttcctgttg gcgcaaaccg atgcggcggt agaagaaccg gttgccgccg acctgtatca
    35761 gaccggtacg gtcgcacaag tcctgcaagt gttgaaactg cccgacggca cggtaaaagt
    35821 attggtcgaa gggctgtatc gcggacgtgt tctgaccatt gaagacacgg gcggtctgtt
    35881 cgtttcccat atagagacgg tcgtggaaga agacacgggc ggcaataccg acctcgaagc
    35941 cgtgcgccgc accctgttgg cgcagtttga acaatacgcc aaactcaata aaaaaatccc
    36001 cgccgaaatt atcggcagca ttaacggcat tgccgaaaac agccggctaa ccgatacggt
    36061 cgcagcgcat ttgcagttga aactggcgca acgccaacag attttggaaa ttcccgaaat
    36121 cggcaaacgg atggaattcc tgctggcaca gctggaatcc gaactcgaca ttatgcaggc
    36181 cgaaaaacgc atacgcggac gcgtcaaacg ccaaatggaa aaatcccagc gcgaatatta
    36241 tctgaacgaa cagattaaag cgatacacaa agaattgggc gaagaagacg aaaacggcga
    36301 actggatgcc ttggaagcag gcatcaaaaa ggcggggatg agcaaagaag cggaagaaaa
    36361 atgcctgtcc gaactgaaaa aactcaaaat gatgccgccg atgtctgccg aatccaccgt
    36421 cgtacgcaac tacatcgaca ccctgctcga actgccgtgg aagaaaaaat cccgcgtcag
    36481 caaagacatc gccaaagccg gattggtgct ggataccgac cactacggcc tggaaaaagt
    36541 caaagaacgg attttggaat acctcgccgt ccaaaaacgt atggacaaac tcaaaggccc
    36601 gattctgtgc ctggtcggcc ctccaggcgt gggcaaaacc tctttgggcg aatccatcgc
    36661 caaagcaacg gggcggaaat atgtccgcat ggctttgggc ggcgtgcgcg acgaaagcga
    36721 aatccgggga caccgccgca cctatatcgg ctccatgccc ggtaagattt tgcagaatat
    36781 ggcaaaagcc ggcgtaaaaa acccattgtt cctgctcgac gaaatcgaca aattgggtag
    36841 cgacttccgg ggcgatcccg ccagcgcgtt gctcgaagtg cttgatcccg agcaaaacaa
    36901 caagttcgcc gatcattatg ccgaagtgga ttacgatttg agcgatgtga tgtttattgc
    36961 gacttcaaac agcctgaata tcccgacacc gctgctcgac cgtatggaaa tcatccgtct
    37021 gtccggctat accgaagacg aaaaaatcaa tatcgcgatg cagtacctcg tgccgaagca
    37081 aatgaagcgc aacggcgtaa aagaagggga attggcaatc gaagaaagcg cggtgcgcga
    37141 tattatccgt tactacaccc gcgaggcggg cgtgcgttcg ctcgaccgcg agattgccaa
    37201 aatctgccgc aaggtggtga tgcagattac cttggacgaa gactcaaaaa ccaaaaaaac
    37261 cggaaaagcc aagcccaggg cggttaaagt aaatgagaaa aatctgcacg actatttggg
    37321 tgtgcgccgc ttcgattacg gcgttgccga aagcgaaaac cgtatcggac aagtaaccgg
    37381 tttggcgtgg acggaagtcg gcggcgaatt gctgaccgtc gaagccgcag cattgccggg
    37441 taagggcgtg attcagtgca ccggccagtt gggcgatgtg atgaaggaat ccgtgtctgc
    37501 agcgtggtcg gttgtccgct cccgcgcgga atcagtgggc ttggctcctg atttttacga
    37561 gaaaaaagac atccatgtcc atgttcccga aggtgcgacg ccgaaagacg gtccgagtgc
    37621 gggcattgcc atgactttgg cgatggtgtc tgcattcacc aaaatcccgg tgcgcgccga
    37681 tgttgccatg acgggcgaaa ttaccctgcg cggcgaagtc ctgccgattg gcggtctgaa
    37741 ggaaaaactg ttggctgcgc ttcgcggcgg catcaaacac gttttaattc caaaagacaa
    37801 cctcaaagac ttggaagaaa tccctgaaaa cgtgaaaacc ggcctgacca tccatccggt
    37861 caaatggata gacgaggtat tggctctggg tttggaaagc cagcctgagc cttgggcaga
    37921 accttctggt gcggaagcgg cggcggaatc cgcttcaaaa ccaaaacccc gcagcagggc
    37981 aaccaaacat tgaaacgcag gaaatgtgtt gtaaaaatgc ggtttcgtcc tgaaagcctg
    38041 tcaaataggg tgattccgta ttttttgctt gacacggcaa tttcagaatt gctataaagc
    38101 gaaagttgct caagcagtac aaaccccgag tattcgggca tttcatcatt ttttaagaca
    38161 ggaagggact gattgtgaac aagtctgaat tgatcgaagc gattgctcaa gaagccgaca
    38221 tttccaaagc cgccgcacaa aaagctttgg atgccactac caatgcagta accaccgccc
    38281 tgaaacaagg cgacaccgtt actttggtcg gtttcggtac tttctacgtg ggcgaacgtg
    38341 cggaacgcca aggccgcaac cccaaaaccg gcgagcctct gaccattgcc gccgccaaaa
    38401 cgcctaaatt ccgcgccggc aaagctttga aagacgcact gtaagccgtt ttttatgaaa
    38461 aaagccgatt ctttaaagaa tcggcttttt tatcggtcca cattattctg atttcaaatc
    38521 ggcaacacac tgcttgtcac gtgcttcaaa ggcatttgcg ccgccgagca ggtcaagctg
    38581 ttcttgtgcg ccgagtttgc cgaaggatct aatctgtttc tcgctcaatc tgtccaaagg
    38641 ctgctcccac atacatttgc agtagtcgac ggcgaggcgg gtattgtttg aatctaaacc
    38701 gcgggcccgc aaatcgtttt gccatttttc ggcaaacgga atattcttca cgcaagactc
    38761 gacaattttc tgttttgcct gcggtttgga catcgcgcat tgggagagca gggcggttaa
    38821 agcgagcaac gccaaaatga cccacgccca aatgcggatg gtgcggattt tggcttttgc
    38881 ttttttgcgc gcggcaacct gctctttcgt cagcatttcg tgtttcggct cagtcatgca
    38941 ggctttccat gcggatcatg gtaatcggtt tttccacgca atccagtgct tcgatggctg
    39001 cgattgccga cttgatgtgt ttttcgaccg tgctgtgggt cagaatcacg atttcggcag
    39061 tggtctgatc aatcacgcct ttttgaatca gtgcttcgat ggacacgttt tcttgtgcca
    39121 acagcgcggc gatttgcccc agcgtgcccg gttcgtcttt ggcttggacg cgcaggtagt
    39181 agctgctggt aatttcgtcc ataggcagga tggtttgcgc ttggacttgc gcgggttgga
    39241 acgccagatg cggtacgcgg tgggcggtat cggcttcaac caggcgggcg atgtcgatga
    39301 tatcggcaac cacggcggaa gcggtcggca atgcgcccgc gcccgcgccg taatataagg
    39361 tttcgccaac catatcggcg ttgacgcgca cggcgttcat cacgccgttg acgtttgcca
    39421 agaggcggct ttcgggaatc agggtagggt ggacgcgcag ctcgatgcct ttgccggttt
    39481 tgcgggtaat gcccaacagt ttgatgcgat agccaagttc ttcggcgtat ttgatgtcgc
    39541 ggctgtcgag tttgctgatg ccttcgaggt agcaggcgga aaagttcatc ggcgtgccga
    39601 atgccagtgc gctcatgatg gtgattttat ggcccgcatc gttgccttcg atgtcgaagg
    39661 tcggatcggc ttcggcataa cccaatgcct gcgcttcttt cagtacatcg gcaaacgcgc
    39721 tgcctttttc gcgcatttcg gagaggatga agttgctggt gccgttaata atgccggcga
    39781 tggatttaat cctgtttgct gccaaacctt cgcgcagggc tttgatgatt gggataccgc
    39841 ccgctactgc ggcttcaaat tgcaccatga catttttttc ttccgccagc gagaagattt
    39901 cgttgccgta ttcggcgagc agttttttgt tggcggtaac gatgtgtttg ccgttttcaa
    39961 tggctttcaa caccgcatct ttggcaatgc cggtaccgcc gaacaattcg acgacgacat
    40021 cgacgtcttc acgcgcgacc agttcgaacg ggtctttgac aaaggctgcg gacgggcaga
    40081 tttggcgggc tttttcttcg ctcaagtcgc atacggcact gatgcgcact tcgcgtccca
    40141 aacggcggct gatttccgcc gcattgtccc gtaacacggc agccgtaccg ccgccgaccg
    40201 tacctaaacc taaaagaccg atgtttactg gcttcattgt gtctccttgt aagccgactg
    40261 aaatgtaaat attgaaagac gaaaatatcc gctgccgata taattgtgcc gcactttgaa
    40321 tcaaatgccg tctgaaatcg gcaggcgggt cagatgaaat ctgccaatcc tacatgaatt
    40381 cgcctgattt tgcacccctt tcggtgtaga tgatgcggcg acggggtaaa aaaatgttgt
    40441 ttgaagaaaa tccgatagac gaacattttg cagagtatga ccgccgcacg ggtgaaatcc
    40501 ggctgtccgg cagacatttc aataagcctg tgctgctgca tagggattct gccggcctct
    40561 cgaaatggca gtcattgtca gaactgactc cggataatct gttgtctgat gtcaaagcgg
    40621 atgactatcc ggaaatattg attatcggga cgggcgcggc tcaggagttt atccatccca
    40681 aaatcatggc ggatttttcc cgaatcggaa tcggcgtgga atgcatgaat accgattcgg
    40741 cattcaggac attggttttc ctgcactcgg aagggcgcag ggcttgggct tggcttcagc
    40801 cgtaaatttc ccatttcaga cggcatcggc actgactttc aggtaaaata cgggcttttt
    40861 ccgcccgacg atgtttccgt tatgattgaa atcaaaaacc tcaccctgca acgcggtttg
    40921 aaagtcctgc tcgacaaagc caacgctacc gtcaatcccg gtcagcgcgt cggtttgatc
    40981 ggcaaaaacg gaacgggcaa atcgagcctg tttgccttaa tcaagggtaa aatcactcag
    41041 gacggcggcg atgtctcgat tccgaaaaac tggcggctcg cttccgtttc ccaagaaacg
    41101 cccgatttgg atatttccgc tttggattac gttttgcagg gcgatgccga gttgcaggct
    41161 tttcagacgg cattgaggca ggcggaagcg caaaatgacg gcatgaagca ggcggaatat
    41221 cacgccaaat tggaagaaat cgacgcttat accgcgccgg cccgtgcagc gaaattgttg
    41281 aacgggctgg gtttttcgca agaagaacac agccgtcccg tcaaatcctt ttccggcggc
    41341 tggcgtatgc gcctcaatct cgcccaagcc ctgatttgcc gcgccgattt gctcttgctt
    41401 gacgaaccga ccaaccactt ggatttggaa accgtcttgt ggctggaaaa ccaccttgcc
    41461 tctttaccct gcacgcaaat catcatttcc cacgaccgcg atttcctcaa tgcggcaacc
    41521 acccaaacca tcgaattgtc ccagcaaaag ctcacgcaat acggcggcaa ttacgatttt
    41581 taccaaaacg aacgcgcgca gcgtctcgcg caacaacaag ctgcctatgt caaacagcag
    41641 gcgcaaatca aacatctgca atcctttatc gaccgcttca aagccaaagc caccaaagcc
    41701 gttcaagcgc aaagccgcat gaaggctttg gcaaagctag aacgcgttgc gcccgcgcat
    41761 ctggacagcg agttttcctt tgagttttac catcccgacc atctgcccaa tcctttgtta
    41821 aagctagaac acgctgattt gggttacgaa ggcaaaaccg ttctgcatga cattaccctg
    41881 tcgctggaaa gcggcgcacg ctacgggcta ttgggtgtca acggcagcgg taaatctacc
    41941 tttatcaaag ccttggcggg caaaatcgat ttgctttccg gcagcatcgt ccgttccgaa
    42001 aaactcaata tcggctattt tgcccaacac caactcgata ccatccgcgc cgaccaaaac
    42061 cctgtttggc atatccagca gctttctccc gaagtacgcg aacaagaaat ccgaaacttc
    42121 ctcggcggct tcaactttgc cggcgatatg gcgttgcaga aaaccgaacc tttttccggc
    42181 ggagaaaaag cccgtctcgc ccttgccatg attatctggc aaaagccgaa cctgctgctg
    42241 cttgacgaac caaccaacca tctggacttg gatatgcgcc acgccctgac gctcgctctg
    42301 caaagtttcc aaggcgcctt aatcatcgta tcgcacgacc gcagcctgct tgaagcgact
    42361 accgacagct tcctcctgat cgacaaaggc cgtctgaaga acttcgacgg cgatttgaac
    42421 gattaccgcc aatggcgttt ggcacaggaa aacgccgccg ccgcacccgc agcatccgca
    42481 caaagccaaa gccgcaaaga caccaagcgc atcgaagcgc aaatccgtca ggaaaaagcc
    42541 cgacgcggca agccgataca gcagaaaata gaccgtgccg aaaaagaaat ggcgcagctt
    42601 tccgaaattc agacggcatg tgaagcattt ttagcacaag aagacgctta ccttgaagca
    42661 aacaaagaaa aattgcaaaa taccttatcc ctgctggcaa aagtcaaaac acaacttgcc
    42721 caaatcgaag aggtttggct ggcttgtcaa gaagaattgg aacggattga aactgaaatc
    42781 gagaaacagt ttgccgagcg ataaagaagc gcggacaggg tgcttatctt tgcctgccaa
    42841 taacggtata attcgggcgt tatcaccgcc ttttaccggt ataaacatca gactttttgc
    42901 cgccttgggt ctcctgtccc tttccggcgc ggcggcgcaa gcctccgtat accattgcaa
    42961 ctcaaatggc aaaagcgtat atacatcagc cccagcagga tcgtgcgccg atgcggattt
    43021 acctaaaatc agcagccatc agggaggcgg ataccgcctg aaaattaaaa aactcagtta
    43081 agaagccaaa atacacatag aaagtaaaaa gaaaaacaaa aaacctgccg ggaaaaagaa
    43141 caagcaggct gccaaagccc cgaaagaaaa tcaaaagcaa ccaaccgaaa agaaaagccc
    43201 gtaaaacgcc aagaaagcat tacaaaaaat accaaaaaat caaataatta tccgagcatc
    43261 aagcacatta tgaaatcaaa actcccctta atcctaatca acttttccct gatttcaagc
    43321 ccattgggtg cgaatgcggc caaaatctac acctgcacaa tcaacggaga aaccgtttac
    43381 accaccaagc cgtccaaaag ctgcctctca accgatttgc ccccaatcgg caactacagc
    43441 agcgaacgct atatcccgcc ccaaacatcc gaaccgacac catcaccgtc aaacggcgga
    43501 caggctgtca aatataaagc cccggtcaaa acagtatcca agccggcaaa atccaatacg
    43561 cccccgcctc aacaagcacc tgtaaacaac agcagacgct ccattctcga agcagaattg
    43621 agcaacgaac gcaaagccct gactgaagcc caaaaaatgt tatcacaagc acgtctggca
    43681 aagggcggca acatcaacca tcaagaaata aatgcattac aaagcaatgt attggacagg
    43741 cagcaaaata tccaagcact gcaaagagaa ttgggacgta tgtaaagccg tgttttcaaa
    43801 tcgaccgttc caaggatttg acagaagaaa tgatgaaaaa gcagcggcat atcctgtgtg
    43861 tcggctgcct gtatattata ttgaaggtca acgtatttcc caataccggc tggatggaag
    43921 catcgaaagg gatgcagggc atgtcgttca gtaccttcgg atacgcggat gctccgggct
    43981 atacattcag ccgcattggc catactttaa ttttcctact tggaaagacc acagggacac
    44041 gtacaactgt ttccgatagt tcgcataatg tatattatgt taaattatat atttgtgcaa
    44101 ataactccaa ggcattaagc tgttgtttga tgctttgcca gtttgggaga aagttgtcca
    44161 tatgcgccat aaaacgggcg ttgcgatggc gttcgagcaa atgggtcagt tcgtggacga
    44221 cgacgtatta agcggtgcgg acacccgaac cgcgatcccc taaatgtctt ggtgggaatt
    44281 taggggattt tggggaattt tgcaaaggtc tcaaaacccg ctcgccttat agcctgtctg
    44341 tgccgccatt tcagcctcca aaacccatat tttcaaggtg ggcattgact ttgctttgcc
    44401 acttttccac ttctcgggca agctcggcca tcgggctgcg gtagcggtct tccagcgtgt
    44461 tcaagcggct gataagctgc tgaatactgt tttccaagcg attttcgatt cggctttgta
    44521 aatcggcaag ccatttgtct tgaacggcaa gctgcttgat ttcggcttcg gaaagtcggc
    44581 cgaattgttt gaatacggca aggttcaggg cttcgatttg ggttttgact gcgtctttcg
    44641 cggctttttc ctgcgtcatc agtgtttggg tggtttgtaa aacagcccgt tcgccttctt
    44701 ctatgccgct ttcttccaat gcggttttca gaagtttggc ggaaagtttg ccttttgcat
    44761 ccaatacgtc gttcagcgca ccctcttcgc cgccgtgttc ttctatgtgg ttttccaatt
    44821 cttggcttag gcgttccagc tcgctttgtt tttcttccag cttggcgatg ccgtctgaaa
    44881 agtagcggcg ggcaaccagc tcgggggcga tgacttcgct gcggtatttt ttgctgatgc
    44941 gcttggtttt ggctttgcct tttttgccgg tttcggtttc ctcaaagacg acggtcaggt
    45001 tcgcggcttc atcgctttct ttggtgattt cggccaggtt tttaaccgcc ttccagccat
    45061 cttgggcgat gagataaaca tcgtcttgca gggtttccgc ccagtagtcc gtcaggattt
    45121 ggtagaaatc gtattcttca atcaggctgc cgggtttgaa cgcgtccagc aggctttcgc
    45181 tccatttccg gataagcctg cccggccggc tgcccggccg gctgcccggt tggatgacgg
    45241 caaggtcgtt ttgagtgtgc cacgcggcaa actttgccag gtgtccggct ttgaaggcgg
    45301 cgtaatcggg gtgcgccaat atatgggctt tgatttggct gctttcgatt ttatagtgga
    45361 ttaaatttgg ggctgtacta gattagccct aaat
//
LOCUS       DAWFHY010000003        44503 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.003, whole genome shotgun sequence.
ACCESSION   DAWFHY010000003 DAWFHY010000000
VERSION     DAWFHY010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 44503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44503
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.003"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            73..1113
                     /gene="murB"
                     /locus_tag="WGQ68_000113"
     CDS             73..1113
                     /gene="murB"
                     /locus_tag="WGQ68_000113"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEZ0714000.1"
                     /translation="MQPIRYRTDLTPYNTFGLRAQARAFIALEHADELCDIVRLPEFD
                     RDTVLWLGGGSNILLMQDYAGLVVHMENKGIREIARSDGIVRIEAQAGEIWHDFVLHT
                     VALGLNGLENLSLIPGTVGASPVQNIGAYGVEAKDVIHSVRCFDLDTETFVTLSNADC
                     RFAYRESLFKQEGKGRYVIVSVVFALKTHFVPNLGYGDLAAAVAELSAGRVPTAKDVS
                     DAVCAIRNSKLPNPNVLGNVGSFFKNPVIGAGKAAALLEQHPDMPHYPQPDGSVKLAA
                     GWLIDQCRLKGFQIGGAAVHGRQALVLVNKNNASANDVRQLAQHIKFTVFARFQVELH
                     AEPNWLPASFSL"
     gene            1125..1775
                     /locus_tag="WGQ68_000114"
     CDS             1125..1775
                     /locus_tag="WGQ68_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEZ0714001.1"
                     /translation="MPVTRPAKINTYTRIIDASLALFNEEGERNISTNHIAAHLGISP
                     GNLYYHFRNKDEIIVQLFKRYSEALLEYLNEAVLPSDVEDSINYMAGIYDVMWEYRFL
                     FSDVNTLLARSAELLGEHNTFTQAKVSPLLVNLLTQLNGLNIIQADQTAMNDLAVNMW
                     MVTKYWFDFDSSLRGRTKLTEDSKARGIRRTLSLLRPYLLPEHRKKYDRKIGNGNP"
     gene            1830..2627
                     /locus_tag="WGQ68_000115"
     CDS             1830..2627
                     /locus_tag="WGQ68_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0714002.1"
                     /translation="MRAVPPPHISITGLGYLGLPLAQKFYQHGSRVAAIKRSLTSDDI
                     NLPIHLDTIDLNQDSAFQSANLARDTSFWRHHANKPVWFCLLPPSSLTHYADTVKQWA
                     ELARACNVQHLIFTSSTSVYGDTARECDEIALPDPQTESARQILAAEQHLLDSGVPNI
                     DILRLGGLYCAERHPVSRLVQKQNIPGGNRPINIVHRNIAVESLFQTAFNPSGRRLKN
                     IIEPRHPTRREFFTEEAAKLGLPAPDFAPDDSVGKIIRTVCDNGLSL"
     gene            2678..3244
                     /locus_tag="WGQ68_000116"
     CDS             2678..3244
                     /locus_tag="WGQ68_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HEZ0714003.1"
                     /translation="MSAFLPIINRLILQSPDSRSELAAFAGKTLTLNIAGLKLAGRIT
                     EDGLLSAGNGFADTEITFRNSAVQKILQGGEPGAGDIGLEGDLILGIAVLSLLGSLRS
                     RASDELARIFGTQADIGSRAADIGHGIKQIGRNIAEQIGRFSREPESANIGNEALADC
                     LDEISRLRDGVERLNERLDRLERDIWID"
     gene            3264..4154
                     /locus_tag="WGQ68_000117"
     CDS             3264..4154
                     /locus_tag="WGQ68_000117"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEZ0714004.1"
                     /translation="MNSPFHNIGIVTRPNTPDIQDTAHTLITFLKQHGFTVYLDEVGI
                     KEGCIYTQDTVGCHIVNKTELGQYCDLVAVLGGDGTFLSVAREIALRAVPIIGINQGH
                     LGFLTQIPREYMTDKLLPVLEGKYLAEERILIEAALIREGKTAERAIALNDAVLSRGG
                     AGQMIEFEVFVNREFVYTQRSDGLIVSTPTGSTAYSLAAGGPIMQAGLHAFTLVPICP
                     QSMTNRPIAIPDTSEIEILVTQGGDARVHFDGQTHIDVQNLDRITIRRYRNPLRILHP
                     TDYQYFKTLRQKLHWGEQLV"
     gene            4179..4937
                     /locus_tag="WGQ68_000118"
     CDS             4179..4937
                     /locus_tag="WGQ68_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0714005.1"
                     /translation="MNPKNPPDHTEDAVRLSKRMAQLGLCSRREADGYIGQGWVTVNG
                     KTAVLGQKVSPADRIELNKKAHEQQAARITILLNKPVGYVSAQAEKGYKSAAELITPE
                     NRWEGDTGRIRFDPKHKIGLAPAGRLDIDSVGLLVLTQDGRIAKQLIGENSGSEKEYL
                     VRVRGKLDEKGLALLNHGLSLDGEKLRPAKVEWQNEDQLRFVLKQGKKRQIRRMCELV
                     GLRVVGLKRIRMGKVKLGRLPPGKWRYLAPGESF"
     gene            complement(4992..5657)
                     /locus_tag="WGQ68_000119"
     CDS             complement(4992..5657)
                     /locus_tag="WGQ68_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0714006.1"
                     /translation="MTVLSKEQVLSAFKNRKSCRHYDAARKISAEDFQFILELGRLSP
                     SSVGSEPWQFIVVQNPEIRQAIKPFSWGMADALDTASHLVVFLAKKNARSDSPFMLES
                     LKRRGVTEPDAVAKSLARYQAFQADDIKILDDSRALFDWCCRQTYIALANMMTGAAMA
                     GIDSCPVEGFNYAEMERILSGQFGLFDAAEWGVSVAATFGYRVQEIATKARRPLEETV
                     IWA"
     gene            complement(5767..6675)
                     /locus_tag="WGQ68_000120"
     CDS             complement(5767..6675)
                     /locus_tag="WGQ68_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HEZ0714007.1"
                     /translation="MQPPKASDIISHLQAGGQQRFFRRHLPAIQYTHLNNYLNIKEKQ
                     RMSAIADVQSSRDLRNLPINQVGIKDLRFPITLQTAEGTQSTVARLTMTVYLPAEQKG
                     THMSRFVALMEQHTEALDFAQLHRLTAEMVALLDSRAGKISVSFPFFRKKTAPVSGIR
                     SLLDYDVCLTGEIKDGAYGHSMKVMIPVTSLCPCSKEISQYGAHNQRSHVTVSLTADA
                     EVGIEEVIDYVETQASCQLYGLLKRPDEKYVTEKAYENPKFVEDMVRDVATALIADKR
                     IKSFVVESENFESIHNHSAYAYIAYP"
     gene            6743..7900
                     /locus_tag="WGQ68_000121"
     CDS             6743..7900
                     /locus_tag="WGQ68_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HEZ0714008.1"
                     /translation="MKFATQAIHSGYDCDEHNRALMPPIYQNSMFALHEIGENVPYRY
                     SRLSNPTRQILEDTVADLEHGAAGFVFSSGMAGIDAVWRTFLRPGDTIVAVADIYGGA
                     YDLLVDVYQKWGVNVVFADLGNPDNLDELLKAHKVKLVWLETPSNPLLRLVDIKALAA
                     KAKAAGALVGIDNTFATPYLQQPLDMGCDFVFHSATKYLCGHSDVLMGIVVAKTKELA
                     QPLHDMMVHTGAVAGPLDCWLVLRGIKTLALRMEAHCKNALEIARRLEDHPAIEKVFH
                     PGLPSHEHYELAQAQMPKGIGGVVTVYLKNDTREAANSVIKNMKLVKMASSLGGVESL
                     VNHCYSQSHSGVPHNVKMEMGIRVGLLRFSVGIEDVDDIWNDISAALDTTV"
     gene            complement(7964..8965)
                     /gene="hemB"
                     /locus_tag="WGQ68_000122"
     CDS             complement(7964..8965)
                     /gene="hemB"
                     /locus_tag="WGQ68_000122"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEZ0714009.1"
                     /translation="MQFPYRNVPASRMRRMRRDDFSRRLMREHTLTADDLIYPVFVLE
                     GSAREEDVPSMPGVKRQSLDRLLFTAEEAVKLGIPMLALFPVVTANKTERAQEAYNPE
                     GLVPSTVRALRERFPELGIMTDVALDPYTVHGQDGLTDENGYVMNDETVEVLVKQALC
                     HAEAGAQVVAPSDMMDGRIGAIREALEDAGHIHTRIMAYSAKYASAFYGPFRDAVGSS
                     GNLGKADKKTYQMDPANTDEALHEVALDIQEGADMVMVKPGLPYLDVVRRVKDEFGVP
                     TYAYQVSGEYAMLQAAVANGWLDGDKVVLESLLAFKRAGADGILTYYAIEAAKMLKR"
     gene            complement(9148..9432)
                     /gene="yhbY"
                     /locus_tag="WGQ68_000123"
     CDS             complement(9148..9432)
                     /gene="yhbY"
                     /locus_tag="WGQ68_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEZ0714010.1"
                     /translation="MTDTKLNTKEILELKARAHHLHPVVMVGQQGLTDAVIKETDAAL
                     TAHELIKVRVFGDDRAERIEICTALCEAVDAQPVQHIGKLLVLWRKNIEG"
     gene            9542..10162
                     /locus_tag="WGQ68_000124"
     CDS             9542..10162
                     /locus_tag="WGQ68_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HEZ0714011.1"
                     /translation="MAVRSKSSKAWLHEHVNDHYVHMAQKDGYRARAAYKLLEINEKD
                     KLIKPGTVLADLGSAPGSWSQVAAKLTGTSGAVFALDILPMEAIGGVSFIQGDFREND
                     VLAQFETLLDNRPLDLVICDMAPNMSGNAVSDQARSFYLCELALDFASQHLKTGGSFL
                     VKVFQGAGYQEYMAAMREIFGTVQTRKPEASRNRSSEIYLLGKNKR"
     gene            10226..12193
                     /gene="ftsH"
                     /locus_tag="WGQ68_000125"
     CDS             10226..12193
                     /gene="ftsH"
                     /locus_tag="WGQ68_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEZ0714012.1"
                     /translation="MGNTFKSILVWVALGIGLMAAFNALDGKKEDNGQIEYSQFIQQV
                     NNGEVSGVNIEGSVVSGYLIKGERTDKSTFFTNAPLDDNLIKTLLDKNVRVKVTPEEK
                     PSALAALFYSLLPVLLLIGAWFYFMRMQTGGGGKGGAFSFGKSRARLLDKDANKVTFA
                     DVAGCDEAKEEVQEIVDYLKAPNRYQSLGGRVPRGILLAGSPGTGKTLLAKAIAGEAG
                     VPFFSISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRQRGAGLGGGN
                     DEREQTLNQLLVEMDGFESNQTVIVIAATNRPDVLDPALQRPGRFDRQVVVPLPDIRG
                     REQILNVHSKKVPLDESVDLLSLARGTPGFSGADLANLVNEAALFAGRRNKVKVDQSD
                     FEDAKDKIYMGPERRSMVMHEDEKRATAYHESGHAIVAESLPFTDPVHKVTIMPRGRA
                     LGLTWQLPERDRISMYKDQMLSQLSILFGGRIAEDIFVGRISTGASNDFERATQMARE
                     MVTRYGMSDKMGVMVYAENEGEVFLGRSVTRSQNISEKTQQDIDAEIRRILDEQYQVA
                     YKILDENRDKMETMCKALMEWETIDRDQVLEIMAGKQPSPPKDYSHNLRENADAAEDN
                     APHAPTREETEAPAPADTASSESEQRSENKA"
     gene            12415..12846
                     /locus_tag="WGQ68_000126"
     CDS             12415..12846
                     /locus_tag="WGQ68_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEZ0714013.1"
                     /translation="MKKVEKNILVLHGADKMFELVDKVEDYPNFLPWYSKTEVIGRNG
                     NELKARLFMDYMHVRQSFATHNRNIPGREIRMELLEGPFKTLRGTWKFIDLGDDMCKI
                     EFNLEYDFSNAVLSALISPVFNHLSATLVEAFVKEADRRYA"
     gene            12839..13117
                     /locus_tag="WGQ68_000127"
     CDS             12839..13117
                     /locus_tag="WGQ68_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HEZ0714014.1"
                     /translation="MLEIEIVYGLPDRQVLKTMQLAEGTTVRAAALQSGLDGIFEDLN
                     LHSAPLGIFGKAVKDDTPLRDGDRIEVYRPLLIDPKEARRKRVQNQEE"
     gene            13170..13748
                     /gene="pth"
                     /locus_tag="WGQ68_000128"
     CDS             13170..13748
                     /gene="pth"
                     /locus_tag="WGQ68_000128"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEZ0714015.1"
                     /translation="MSNTIKMVVGLGNPGKEYEQTRHNAGFWFLDELAWKWKASFKEE
                     KKFFGEVARATLPDGDVWLLKPTTFMNRSGQAVAALAQFYKIKPEEILVVHDELDIPC
                     GRIKFKLGGGNGGHNGLKDIQAKLGTADYYRLRLGIGHPGDRNLVVGYVLNKPSTEHR
                     RQIDDAVAKSLQAIPDILADKCEEATRFLHSK"
     gene            13809..14015
                     /locus_tag="WGQ68_000129"
     CDS             13809..14015
                     /locus_tag="WGQ68_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HEZ0714016.1"
                     /translation="MTELKQLIQTESIPVIEETLDFLLYECSIDDAPSAEEVAQWRDI
                     LAARGGKFLRLSKICQTWLDEEAA"
     gene            14012..14374
                     /locus_tag="WGQ68_000130"
     CDS             14012..14374
                     /locus_tag="WGQ68_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEZ0714017.1"
                     /translation="MKLPRNRFSLLSALWFAGGIYSLLFKAADTAPPPFPHFDKAAHL
                     ALFFAQIWLLTKAFKTGKLPIPYRSLMVFALCFALFSECAQAWFTATRTGSLGDVLAD
                     MAGTVLALFAARAADRPD"
     gene            14605..16020
                     /locus_tag="WGQ68_000131"
     CDS             14605..16020
                     /locus_tag="WGQ68_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13/DASS family transporter"
                     /protein_id="HEZ0714018.1"
                     /translation="MNLHAKDKTQHPENVELLSAQKPITDFKGLLTTIISAVVCFGIY
                     HILPYSPDANKGIALLIFVAALWFTEAVHITVTALMVPILAVVLGFPDMDIKKAMADF
                     SNPIIYIFFGGFALATALHMQRLDRKIAVSLLRLSRGNMKVAVLMLFLVTAFLSMWIS
                     NTATAAMMLPLAMGMLSHLDQEKEHKTYVFLLLGIAYCASIGGLGTLVGSPPNLIAAK
                     ALNLDFVGWMKLGLPMMLLILPLMLLSLYVILKPNLNERVEIKAESIPWTLHRVIALL
                     IFLATAAAWIFSSKIKIAFGISNPDTVIALSAAVAVVVFGVAQWKEVARNTDWGVLML
                     FGGGISLSTLLKTSGASEALGQQVAATFSGAPAFLVILIVAAFIIFLTEFTSNTASAA
                     LLVPIFSGIAMQMGLPEQVLVFVIGIGASCAFMLPVATPPNAIVFGTGLIKQREMMNV
                     GILLNILCVVLVALWAYAVLM"
     gene            16139..16648
                     /locus_tag="WGQ68_000132"
     CDS             16139..16648
                     /locus_tag="WGQ68_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0714019.1"
                     /translation="MIILHTNKGDIKIELDFDKAPVTAKNFEQYVKDGFYDGVIFHRV
                     IKGFMIQGGGMDENMNEKETRDPIQNEASNGLPNDKYTIAMARTSDPHSASAQFFINT
                     ADNAFLNFRSKELYGKTVVQDWGYAVFGKVVDGFDVVDAIEGVSTKRHGYHDDVPSEP
                     VVIIKAEAV"
     gene            16811..18193
                     /locus_tag="WGQ68_000133"
     CDS             16811..18193
                     /locus_tag="WGQ68_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEZ0714020.1"
                     /translation="MANIARDIFKAYDIRGIVGKTLTDDAAYLIGRAIAAKAAEKGMT
                     RIALGRDGRLSGPELMEHIQRGFTDSGIGVLNVGMVATPMLYFAAINECGGSGVMITG
                     SHNPPDYNGFKMMLGGDTLAGEAIQELLSIIEKDGFAAAGKQGSVTEKDISGEYHNHI
                     VGHVKLKRPMNIAIDAGNGVGGAFAGKLYRALRCGVTELFCDVDGNFPNHHPDPSKPK
                     NLQDLIAALKNGDAEIGLAFDGDADRLGVVTKDGNIIYPDRQLMLFAQDVLSRNPKAK
                     VIFDVKSTRLLAPWVKEHGGEPVMEKTGHSFIKSAMKKTGALIAGEMSGHIFFKERWF
                     GFDDGLYAGARLLEILSAFDNPSEVLNNLPQSISTPELNIDLPEGGNGHKVIEELAAK
                     AKFEGATEIITIDGLRVEFPDGFGLMRASNTTPILVLRFEADTQEAIERIQNQFKAVI
                     ESNPNLIWPL"
     gene            complement(18249..19004)
                     /locus_tag="WGQ68_000134"
     CDS             complement(18249..19004)
                     /locus_tag="WGQ68_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714021.1"
                     /translation="MIKIRNIHKTFGENTILRGIDLDVCKGQVVVILGPSGSGKTTFL
                     RCLNALEMPEDGQIEFDNERPLKIDFSKKPSKHDILALRRKSGMVFQQYNLFPHKTAL
                     ENVMEGPVAVQGKPAAQAREEALKLLEKVGLGDKVDLYPYQLSGGQQQRVGIARALAI
                     QPELMLFDEPTSALDPELVQDVLNAMKELAREGWTMVVVTHEIKFALEVATTVVVMDG
                     GVIVEQGSPKELFDHPQHERTRKFLSQIQSAKI"
     gene            complement(19014..19730)
                     /locus_tag="WGQ68_000135"
     CDS             complement(19014..19730)
                     /locus_tag="WGQ68_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0714022.1"
                     /translation="MFNNFLASLPFMTETRADMIVSAFLPMVKAGFAVSLPLAAASFV
                     IGMMIAVAMALVRIMPAGGIVRKILLKLVEFYISVIRGTPLLVQLVIVFYGLPSVGIY
                     IDPIPAAIIGFSLNVGAYASETIRAAILSVPKGQWEAGFSIGMTYMQTFRRIVAPQAF
                     RVAVPPLSNEFIGLFKNTSLAAVVTVTELFRVAQETANRTYDFLPVYIEAALVYWCFC
                     KVLFLIQARLEKRFDRYVAK"
     gene            complement(19720..20547)
                     /locus_tag="WGQ68_000136"
     CDS             complement(19720..20547)
                     /locus_tag="WGQ68_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0714023.1"
                     /translation="MMLKKFVLGGIAALVLAACGGSEGGSGASSAPAQSAVSGSLIER
                     INNKGTVTVGTEGTYAPFTYHDKDGKLTGYDVEVTRAVAEKLGVKVEFKETQWDSMMA
                     GLKAGRFDVVANQVGLTSPERQATFDKSDPYSWSGAVLVARNDSNIKSIDDIKGVKTA
                     QSLTSNYGEKAKAAGADLVAVDGLAQSLTLIEQKRADATLNDELAVLDYLKKNPNAGV
                     KIVWSAPADEKVGSGLIVNKGNDEAVAKFSTAINELKADGTLKKLGEQFFGKDISVQ"
     gene            20784..21692
                     /locus_tag="WGQ68_000137"
     CDS             20784..21692
                     /locus_tag="WGQ68_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HEZ0714024.1"
                     /translation="MNTPQSAIIPDHAQAGIFIEADFAANRLNDIKAACRASLDALSA
                     LKTRFPDDILGLTIAFGSKAWATFGHTDEGSEIKPFSEMGNGLAPSTQHDMYIHIQSF
                     RQNAAYALAQSVLGAFGDSICVASEEHGLRLYQDRGLDGFVDSTENPQGDEKIREVAI
                     IPEGLPDAGGSYVLLQKYLHDLKKWDAVPVAEQEASVGRSKETDDEFSRDVRLPDSHL
                     GRVNLKENGVGLKIVRRSLPFGKISGEHGLMFTAYCRTLHNIEAQLLSMFGDTDGKTD
                     LLLRHLSAAVSGGYYYAPSVERLQNL"
     gene            21772..25386
                     /gene="recB"
                     /locus_tag="WGQ68_000138"
     CDS             21772..25386
                     /gene="recB"
                     /locus_tag="WGQ68_000138"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HEZ0714025.1"
                     /translation="MSASIQAFDPLTVPISGTNLIEASAGTGKTYGIAALFTRLIVLE
                     QKSVERVLVVTFTKAATAELKTRLRARLDDVLQVLESKEIAKLGDDTLSDGIAAYCAE
                     HHEGDTFLPALLKQALQKEGRTRLIVRLKAAIGQFDNAAIYTIHGFCQRILRDYAFLC
                     QAPFDVELTEEDGDRLLIPAQDFWRERVSNDPVLAALAFKRKAVPQTVLAQIRAYLSR
                     PYLNFRRPQADLKQAQRNAETSWQTVCRLLPELEAGFWRIHPDLNGNSYRKNSFGNLF
                     KELAQKSAAGQLPFLDKDTHDRLLKLASDKLEAGLKKGKTPDAAVFAELQKLVDFGYD
                     LNALEEAEEATIIRLQLDLIGYLNRSLAEMKKSRRERGFDDLLLDVHTALTDNPHAET
                     LARAVAENWETALIDEFQDTDPLQYEIFQKIFIAQNRPLFLVGDPKQAIYSFRGADIY
                     AYLQAAEDARHRYTLATNYRSHAALIGSIGALFRLKERPFVLENIGYSEVGAARAESR
                     LSPERPAVQVRWLHENDNEKANKDVLRRRAADYCADEIAHALNEAARGRLNFKGCPLQ
                     SGDIAVLVRTHNEAVMVSAALKKRQVQSVLLSRESVFASPEAAALSALIGFWLEPRRA
                     GTLRFVLTSSIFGYDAQQLHDFNQNESEILHWAESARTALDNWNKYGIFAAMQQFSQT
                     HGIETRLLSRNNGRSLTNYFQLLELLAAEDAQNRNPAALHKWLGDQISLAENNSGDNR
                     AIRLESDEDLVKIVTMHASKGLQYPLVYCPFAWDAQDTGPSDWQILHQGANRTELLAK
                     AQLSEDEQKQYADEEMAERLRLLYVALTRAEEQLNIYAAYSTNTADNPLAYLIEGSPQ
                     DSRETVRRAYACEKDGIAMLKRNWRRVADNAPSGTNFAFTENAPPPAAYRSNAGQTAE
                     FAANSIPERGFRFVRHTSFTALSRHTQTPDGGEEDACPSLDAAETSVPAMPSETPTAS
                     DGISIHDFPKGTQAGLCLHEILEDFKFGQAAAGQETLIADKLKKYGFEEIWLPAVAEM
                     AEACRKTPLTGAYDLSDISPECRCPEMGFTLHTEDFSLKRLRDWFARDDIRLPEVCRA
                     AAETLDFHTVNGFLNGFVDMVCQDPDGNICVIDYKSNHLGTDASAYTQQAMDEAVAHH
                     HYYLQALIYAVAAARYFKLRGQPPAAVSVRYLFLRGLDGKGGGVWRWDIDAAALEQIK
                     "
     gene            25425..25784
                     /locus_tag="WGQ68_000139"
     CDS             25425..25784
                     /locus_tag="WGQ68_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEZ0714026.1"
                     /translation="MNIKHLITAALIASAAFAAQAAPQKPVSAAQTAQHPAVWIDVRS
                     EQEFSEGHLHNAVNIPVDQIVRRIHEAAPDKDTPVNLYCRSGRRAEAALQELKKAGYT
                     NVANHGGYEDLLKKGMK"
     gene            26026..26505
                     /locus_tag="WGQ68_000140"
     CDS             26026..26505
                     /locus_tag="WGQ68_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HEZ0714027.1"
                     /translation="MKHILPLIAASALCISTASAHPASEPSTQNETAMTTHTLTSKYS
                     FDETVSRLETAIKSKGMDIFAVIDHQEAARRNGLTMQPAKVIVFGTPKAGTPLMVKDP
                     AFALQLPLRVLVTETDGKVRAAYTDTRALIAGSRIGFDEVANTLANAEKLIQKTVGE"
     gene            complement(26781..28160)
                     /gene="radA"
                     /locus_tag="WGQ68_000141"
     CDS             complement(26781..28160)
                     /gene="radA"
                     /locus_tag="WGQ68_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEZ0714028.1"
                     /translation="MAKTLKTLYQCTECGGTSPKWQGKCPHCGEWNTLQESLAAPEPK
                     NARFQSWAADTSTVQSLSAVTATEVPRNPTGMGELDRVLGGGLVDGAVILLGGDPGIG
                     KSTLLLQTIAKMAQSRKVLYVSGEESAQQVALRAQRLELPTDGVNLLAEIRMEAIQAA
                     LKQHQPEVVVIDSIQTMYSDQITSAPGSVSQVRECAAQLTRMAKQMGIAMILVGHVTK
                     DGAIAGPRVLEHMVDTVLYFEGDQHSNYRMIRAIKNRFGAANELGVFAMTENGLKGVS
                     NPSAIFLASYRDDTPGSCVLVTQEGSRPLLVEIQALVDDAHGFTPKRLTVGLEQNRLA
                     MLLAVLNRHGGIACFDQDVFLNAVGGVKIGEPAADLAVILAMLSSFRNRPMPEKTVVF
                     GEIGLSGEVRPVARGQERLKEAEKLGFKRAIVPKANMPRNAKEFPNLKIYGVSSLQEA
                     IDICRDSRE"
     gene            complement(28346..29410)
                     /locus_tag="WGQ68_000142"
     CDS             complement(28346..29410)
                     /locus_tag="WGQ68_000142"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEZ0714029.1"
                     /translation="MISLKNDTFLRALLKQPVEYTPIWMMRQAGRYLPEYKATRAKAG
                     SFLDLCKNTELATEVTIQPLERFDLDAAILFSDILTVPDAMGLGLYFAEGEGPKFKRA
                     LQHEADIAKLHVPDMEKLQYVFDAVTSIRKALDGRVPLIGFSGSPFTLACYMVEGGGS
                     KEFRTIKTMMYSRPDLLHKILDTNAQAVTAYLNAQIDAGAQAVQIFDTWGGVLSDAAF
                     KEFSLKYIRQIVAGLKRESEGRRVPVIVFAKGGGLWLESMAQIGADALGLDWTCNIGE
                     ARRRVGKQVALQGNFDPFALFGTPESIRTEVARILADYGHGSGHVFNLGHGINQHADP
                     EHAKILVDTVHELSRQYHGG"
     gene            complement(29564..30004)
                     /locus_tag="WGQ68_000143"
     CDS             complement(29564..30004)
                     /locus_tag="WGQ68_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003778183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714030.1"
                     /translation="MGSSDSFKEKKEIFEIGTPAYRQKLIDVWKKSINGNEKSWVLFE
                     NGTCVILLEPEKDLAKQAKEMLSKWGKVQIGTPSADFGIITLDSGDGYAVSCHHPEIF
                     TLILKEEGLDEDFKIGIEGRSHRDCDAEEPKVIHIEDKRTIETP"
     gene            complement(30037..31260)
                     /locus_tag="WGQ68_000144"
     CDS             complement(30037..31260)
                     /locus_tag="WGQ68_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis protein HemY"
                     /protein_id="HEZ0714031.1"
                     /translation="MKTVVWIVVLFAAAVGLALASGIYTGDVYIVLGQTMLRINLHAF
                     VLGSLIAVVVWYFLFKFIIGVFNIPEKMQRFGSARKGRKAALALNKAGLAYFEGRFEK
                     AELEASRVLGNKEAGDNRTLALMLGAHAAGQMENIELRDRYLAEIAKLPEKQQLSRYL
                     LLAESALNRRDYEAAEANLHAAAKMNANLTRLVRLQLRYAFDRGDALQVLAKTEKLSK
                     AGALGKSEMERYQNWAYRRQLADAADAAALKTCLKRIPDSLKNGELSVSVAEKYERLG
                     LYADAVKWVKQHYPHNRRPELLEVFVESVRFLGERDQQKAIDFADAWLKEQPDNALLL
                     MYLGRLAYGRKLWGKAKGYLEASIALKPSISARLVLAKVFDEIGEPQKAEAQRNLVLE
                     AVSDDERHAALEQHS"
     gene            complement(31257..32591)
                     /locus_tag="WGQ68_000145"
     CDS             complement(31257..32591)
                     /locus_tag="WGQ68_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HEZ0714032.1"
                     /translation="MGEPENKSSEPVREIQASKEMPSETSSPRKENETEVHIPAAPFI
                     VKQSGSNALAVCALVLAALGLGTSGFLFVQGQNVLKNQELAFNQKIDKAALGESENAA
                     LLKDNLNRQAAIQSELDRLDGNVKANGEQILEMQKSYRELTKGRADWLVDETETILNL
                     AAQQLVLTGNIQTAVGVLEHIDSRLSRFNQAELLPIKQAVSSDLAELKNRPYVDISGT
                     ALRLDRLETAVSGLPLMLDGVLKPGVQVKNEAASASWWQNVWEKSLGTLKGLVEIRRL
                     ENNDAMLISPEQAYFVRENLRLRLLDARTALMQRNSEVYQGDLNNAEAAVRQYFDAKS
                     PATQSWLKELAELKALDVRMTADDGLKNSLNAVRAYRDGTRMTAAENQEAEQAASEPA
                     NENTASEPAAASDVKTIEAPSLPSERKPEQPAKKQSVPEKAGRSPSAKGERA"
     gene            complement(32606..33367)
                     /locus_tag="WGQ68_000146"
     CDS             complement(32606..33367)
                     /locus_tag="WGQ68_000146"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEZ0714033.1"
                     /translation="METAKPVMLIVRPSGRAAEDVETCLNAGWRAEVLSPVEIEADAA
                     GLELLSEQYARADAVFWVSPTAVETAVPYLNLSDGIKMHIAVGKGSRRALERCLGRTV
                     IAPDDGNDSEAVLRLPVWNSLPEGARVLFVRGHGGRDFLMNALQEKGLRTEMAEVYFR
                     RHKPLNFQNFQTENIAAAYITSTELVRLLFGQLPPQFSRFFKSLLYFTHHPRIAEALK
                     REGVCSVETVPTLEAALSHSSISVSDGMVFPGTSN"
     gene            complement(33388..33807)
                     /locus_tag="WGQ68_000147"
     CDS             complement(33388..33807)
                     /locus_tag="WGQ68_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714034.1"
                     /translation="MKKMFLSAVLLLSAATRTVWADTVFSCKTDNNKYIEVQKINRNL
                     YEYSFGSAAKKEIAIRNSKADLLGRSDRWQGMGSGRWATMKFQNGEFMYTIWTGFDSV
                     THTESSGVVVERRGKEVARVGCTPKTAQANFNDDDFS"
     gene            complement(34058..34378)
                     /locus_tag="WGQ68_000148"
     CDS             complement(34058..34378)
                     /locus_tag="WGQ68_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2322 family protein"
                     /protein_id="HEZ0714035.1"
                     /translation="MNFQDYLATFSSIDHLGGLDVQDADGKTVHHIPAVQGKLGSLKL
                     YNALAERFDGKLDKEAAEQGLIWFAEHVADARAHPGKHPNIDLLEKVVQSGETLLLKP
                     LAAQ"
     gene            complement(34401..35027)
                     /gene="upp"
                     /locus_tag="WGQ68_000149"
     CDS             complement(34401..35027)
                     /gene="upp"
                     /locus_tag="WGQ68_000149"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEZ0714036.1"
                     /translation="MNVNVINHPLVRHKLTLMREADCSTYKFRTLAIELARLMAYEAS
                     RDFEIEKYLIDGWCGQIEGDRIKGKTLTVVPILRAGLGMLDGVLDLIPTAKISVVGLQ
                     RDEETLKPVSYFEKFVDSMDERPALIIDPMLATGGSMVATIDLLKAKGCKNIKALVLV
                     AAPEGVKAVNDAHPDVTIYTAALDSHLNENGYIIPGLGDAGDKIFGTR"
     gene            complement(35133..35444)
                     /gene="grxD"
                     /locus_tag="WGQ68_000150"
     CDS             complement(35133..35444)
                     /gene="grxD"
                     /locus_tag="WGQ68_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEZ0714037.1"
                     /translation="MASIHDQIKEVVTTHRVVLFMKGTKQFPQCGFSSRAVQILNAAG
                     CTDYVTVNVLENPEVRQGIKEYSDWPTIPQLYVNGEFVGGSDILMEMYEAGELQELLK
                     A"
     gene            complement(35545..35760)
                     /locus_tag="WGQ68_000151"
     CDS             complement(35545..35760)
                     /locus_tag="WGQ68_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HEZ0714038.1"
                     /translation="MKAIHPYACPRCCRLPANTFRTGMANSASKFCIAKGGRREVKKD
                     ESGGGYALCHLPDSRIVEEWEYFRSQH"
     gene            complement(35786..36565)
                     /locus_tag="WGQ68_000152"
     CDS             complement(35786..36565)
                     /locus_tag="WGQ68_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0714039.1"
                     /translation="MHIIDSHCHLNFEGLKERLPEVLSNMEANGVGQALAISVSRESF
                     SEVFAIAEAHEHIYCTIGVHPDSKEAEEFSIAEMVAAAAHPKVVGIGETGLDYYWCKG
                     DLSWQHKRFADHIEAANQTGLPVIVHTRDAAADTLSILKECRVNSGVIHCFSEDIGFA
                     RAAMDLGLYISFSGIVTFKNAPLVQEAAKYVPDDRILVETDAPFLAPVPKRGRQNEPA
                     FVRYTAEHIAKLRNQTLEQVAAYTTENFYRLFQKVPDMRTV"
     gene            complement(36570..36920)
                     /locus_tag="WGQ68_000153"
     CDS             complement(36570..36920)
                     /locus_tag="WGQ68_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilZ"
                     /protein_id="HEZ0714040.1"
                     /translation="MSDGQNIPAKMMSLQLKDMNLLYSSYMPFLEHGGLFVQTNDVFS
                     IGDDILLAVEILNFPKLFLPTKVAWINPARTSSKPKGVGLAFTKHENCLKVKDQIEVE
                     LGNTIGGSRPTFTM"
     gene            complement(36924..37901)
                     /locus_tag="WGQ68_000154"
     CDS             complement(36924..37901)
                     /locus_tag="WGQ68_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEZ0714041.1"
                     /translation="MIYPWHNEQWRQIAEHWERRPNAWLFAGKKDTGKTTFARFAAKA
                     LLCETPAPGCKPCGECMSCHLFGQGSHPDFYEITPLSDEPENGRKLLQIKIDAVREII
                     DNVYLTSVRGGLRVILIHPAESMNVQAANSLLKVLEEPPPQVVFLLVSHAADKVLPTI
                     KSRCRKMVLPAPSHEEALAYLRERGVAEPEERLAFHSGAPLFDEADGVRALRIKLLDI
                     LAEPRLLKILDYAALFDKEKLPLAVFVGWMQKWLVDLGLCLQHMKPVYYPAYEDRLLQ
                     TVSGFRPRNVFAAEDMLKQLAPYGFHTLNVKMQIEHLLINYLELKKENG"
     gene            complement(37935..39047)
                     /locus_tag="WGQ68_000155"
     CDS             complement(37935..39047)
                     /locus_tag="WGQ68_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0714042.1"
                     /translation="MTAKEELFAWLRHMNQNKGSDLFVTTHFPPAMKLDGKITRITDE
                     PLTAEKCMEIAFSIMSAKQAEEFSSTNECNFAISLPDTSRFRVNAMIQRGATALVFRA
                     ITSKIPKFESLNLPPVLKDVALKKRGLVIFVGGTGSGKSTSLASLIDYRNENSFGHII
                     TIEDPIEFVHEHKNCIITQREVGVDTENWMAALKNTLRQAPDVILIGEIRDRETMDYA
                     IAFAETGHLCMATLHANSTNQALDRIINFFPEERREQLLTDLSLNLQAFISQRLVPRD
                     GGKGRVAAVEVLLNSPLISELIHNGNIHEIKEVMKKSTTLGMQTFDQHLYQLYEKGDI
                     SLQEALKNADSAHDLRLAVQLRSRRAQSSSPDLELL"
     gene            39233..39934
                     /locus_tag="WGQ68_000156"
     CDS             39233..39934
                     /locus_tag="WGQ68_000156"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEZ0714043.1"
                     /translation="MSLKTVAVIGAMEQEIELLREMMENVKAVSFGKFAAYEGELAGK
                     RMVLALSGIGKVNAAVATAWIIREFAPDCVINTGSAGGLGKGLKVGDVVVGTEIAHHD
                     VDVTAFGYVWGQVPQLPAVFVSDGLLVGKAKQAAEVFEGAAVEQGLIVSGDRFVHSSE
                     GVAEIRKHFPKVKAVEMEAAAIAQTCHQLETPFVIIRAVSDSADEKADISFDEFLKTA
                     AASSAKMVAEIVKSL"
     gene            40076..41869
                     /gene="lepA"
                     /locus_tag="WGQ68_000157"
     CDS             40076..41869
                     /gene="lepA"
                     /locus_tag="WGQ68_000157"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEZ0714044.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLADRFIQYCGGLDLREMSTQVLDSM
                     DIEKERGITIKAQTAALNYKARDGQVYQLNLIDTPGHVDFSYEVSRSLSACEGALLVV
                     DASQGVEAQTVANCYTAIDLGVEVVPVLNKIDLPAADPERVEQEIEDIIGIDAVGAVQ
                     CSAKSGIGVEDVLEEIVAKIPAPTGDENAPLQAVIVDSWFDNYVGVVMLIRVKNGTIK
                     LKDKVRFMSTKAETQVEQLGVFTPKSVQKQELKAGEVGFLITGVKELGQAKVGDTVTL
                     VANPASEPLPGFQEVQSQVFAGLYPVESHDYEALRDALEKLQLNDASLKFEPEVSQAL
                     GFGFRCGFLGLLHLEIVQERLEREFDMDLITTAPTVVYEVVLKSGEKIEVENPSKLPD
                     IGSIETILEPIITATILVPQEYVGNVMTLCNQKRGVQVNMQYMGRQVMLTYDLPMNEV
                     VMDFFDKLKSTSRGYASLDYHFKEFQPSDLIKLDIMVNGEKVDALSLIVHRQSAVHRG
                     RELASKMRELIPRQMFDIAVQAAIGSQIIARENVKALRKNVLAKCYGGDITRKKKLLE
                     KQKAGKRRMKQVGNVEIPQSAFLAILQVSDK"
     gene            42012..43031
                     /gene="lepB"
                     /locus_tag="WGQ68_000158"
     CDS             42012..43031
                     /gene="lepB"
                     /locus_tag="WGQ68_000158"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEZ0714045.1"
                     /translation="MNTMLMSGAAAALLAGIILYFKSDKKRQENGEWSSGLEYAYILT
                     AVGVFAALSLFMSFTAVFLIFVVLCGTAWGVYKYRLRTHPEISESSHFGDYFGSFFPT
                     VLVLFLIRSFIAEPFQIPSSSMRPGLIKGDFILVGKFSYGLRVPVLNNVFIPTGKIER
                     GDVVVFNYPLQPEMTYIKRIVGIPGDVVEYRNKVLTVNGKPTSDIPDGTYRYPDDTDP
                     SEIHNTDMFRSGLDGKSFNILKKEGQPAVSLPVLGKYTSDIMSENGYSIEQSGLEHCQ
                     YADDGSGFVCKVPEGRYFAMGDNRDNSADSRYWGFVDDKLVVGKAMFILMNFGDFGRA
                     GTAIR"
     gene            complement(43250..43399)
                     /locus_tag="WGQ68_000159"
     CDS             complement(43250..43399)
                     /locus_tag="WGQ68_000159"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714046.1"
                     /translation="MNWQAVKTGKTKHAGNGAERKMPSEALSDGIGAGRIKKAASAGM
                     AMQLK"
     gene            complement(43445..44350)
                     /locus_tag="WGQ68_000160"
     CDS             complement(43445..44350)
                     /locus_tag="WGQ68_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0714047.1"
                     /translation="MGKGGDIVVGNGKKYAAPLLVLGCVVFGLGSLIVRSVPVGSYAI
                     AFWRLLISVFVFWFLARFFRQKFPKNRKTVRYALTAGVFLAFDLALWHESIHAVGPGI
                     STLLNSLQIFFLSAIGVFFFGERLSGLKKAGLISAVAGVAMIAGAEFGYNGNAVWGFV
                     SGLVSGLMLALSMVFVRKTHEIETVALFPSMMILSLGGAVSLVVPALLMDGGALYPTT
                     WKDAGLVLVYGVVMQCFAWAMVAYAIPLLSLSLTGLLLLSEPVAALFIDYFGLGKPIE
                     GVQWAGVALTLSAIYLGSLKQQSEP"
BASE COUNT        10659 a        12800 c        11232 g         9812 t
ORIGIN      
        1 gggcaactct aacttaattt gaatttccct agttatctag gacagcccca aacatatttt
       61 aaagctcaaa ccatgcaacc catccgatac cgaaccgacc tcacccccta caacaccttc
      121 ggtcttcgcg cccaagcccg agcctttatt gcgcttgaac acgccgacga gttgtgcgac
      181 atcgtccgac tgcccgagtt cgaccgcgat actgttttat ggctgggcgg cggcagcaac
      241 atccttttga tgcaggatta cgccggactg gtcgtacaca tggaaaacaa aggcatacgc
      301 gagattgcgc gttcagacgg cattgttcgc atcgaagcgc aggcgggcga aatctggcac
      361 gattttgtcc tgcacaccgt cgcgctgggc ttgaatgggc tggaaaacct gagcctgatt
      421 ccgggtacgg tcggcgcgtc gcccgtgcag aacatcggcg catacggcgt agaggcgaaa
      481 gatgtgattc acagcgtgcg ctgcttcgat ttggatacgg aaacctttgt aaccctttcc
      541 aatgccgact gccgcttcgc ctaccgcgaa agcctgttca agcaggaagg taaagggcgt
      601 tatgtgattg tttcggtcgt atttgcatta aaaacgcatt ttgtgccgaa cttgggttac
      661 ggcgatttgg cggccgccgt tgccgaactg agcgcgggca gggtgccgac ggcgaaagat
      721 gtttccgatg cagtgtgtgc aatccgcaac agtaaacttc ctaatcctaa cgtgcttggc
      781 aatgtcggca gtttctttaa aaatcccgtc atcggcgcgg gaaaagctgc cgccctgttg
      841 gagcagcatc ccgatatgcc gcattatccg cagcccgacg gttcggtcaa actcgccgcc
      901 ggctggctga tcgaccaatg tcgtctgaaa ggctttcaaa tcggcggcgc ggcggtacat
      961 ggcaggcagg ctttggtctt agtgaacaaa aacaacgcct cggcaaacga tgtccggcaa
     1021 ttggcgcaac acatcaaatt tacagtattt gctcggtttc aggtagaatt gcacgccgaa
     1081 cccaattggc tgcctgcttc gttcagcctg taaatccaag gagtatgccc gtgacccgcc
     1141 ccgccaaaat caatacttac acacgcatca tcgacgccag ccttgcgctc ttcaacgagg
     1201 aaggcgagcg caacatcagc accaaccata ttgccgccca cttgggcatc agtccgggca
     1261 acctctatta ccacttccgc aacaaagacg aaatcatcgt ccaactgttc aaacgttaca
     1321 gcgaagccct gctggaatac ctcaacgaag ccgtgttgcc gtctgatgtg gaagactcca
     1381 tcaactatat ggcgggcatt tacgacgtga tgtgggaata ccgcttccta ttcagcgacg
     1441 tgaacaccct gcttgcacgc agtgccgaac tattgggcga acacaatacc ttcacccaag
     1501 ccaaagtctc cccgctcttg gtcaacctgc tcacccagct caacggtctg aacatcatcc
     1561 aagccgacca aaccgctatg aacgacctcg ccgtcaatat gtggatggtc acgaaatact
     1621 ggttcgactt cgacagctcc ctgcgcggcc gcaccaagct gaccgaagac tccaaagcac
     1681 gcggcatccg ccgcacctta agcctccttc gtccttatct tttgcccgaa caccgcaaga
     1741 aatacgaccg gaaaatcggc aacggcaacc cgtaaaccca cagcgtccga atcagccttc
     1801 agacggcact tacccgacgg tatcagcgaa tgcgcgcagt ccccccgccc catatctcca
     1861 tcacaggcct aggttacctc ggcctgccgc tggcacaaaa gttttaccaa cacggcagcc
     1921 gcgtcgccgc cataaaacgc agcctgactt cggacgacat caacctgccc atacacctcg
     1981 ataccatcga cctcaatcaa gacagcgcgt ttcaaagcgc gaaccttgcc cgagatacaa
     2041 gcttttggcg gcaccatgcc aacaaacccg tttggttctg cctcttgccg ccatcatcgc
     2101 tgacacatta cgccgatacc gtcaaacaat gggcagaact tgcccgggcg tgcaacgtgc
     2161 aacacctgat tttcacaagc agtaccagcg tttacggcga tacagcgcgc gaatgcgacg
     2221 aaatcgccct acccgatccg caaaccgagt ccgcccgcca aatcctcgcc gccgaacaac
     2281 acctgctcga cagcggcgtt ccgaacatcg acatcctgcg gctgggcggg ctttattgcg
     2341 ccgaacgcca tcccgtcagc cgccttgttc aaaagcaaaa catcccgggc ggcaaccgcc
     2401 ccatcaacat cgtccaccgt aatatcgccg tcgaaagcct gtttcagacg gcatttaacc
     2461 ccagcggcag gcggctgaaa aacattatcg aaccgcgcca cccgacacgc cgcgaatttt
     2521 ttacggaaga agccgccaaa ctcggcctgc ccgcgccgga tttcgcaccc gacgacagcg
     2581 tgggcaaaat catccgtacc gtttgcgata acggcttaag cctgtaaaat aagcggcaac
     2641 agcaacaaac aggcattctc cgccccaatc agaaaatatg tccgccttcc tccccatcat
     2701 caaccgcctg attctgcaaa gcccggacag ccgctcggaa cttgccgcct ttgcaggcaa
     2761 aacactgacc ctgaacattg ccgggctgaa actggcggga cgcatcacgg aagacggttt
     2821 gctctcggcg ggaaacggct ttgcagacac cgaaattacc ttccgcaaca gcgcggtaca
     2881 gaaaatcctc caaggcggcg aacccggggc gggcgacatc gggctcgaag gcgacctcat
     2941 cctcggcatc gcggtactgt ccctgctcgg cagcctgcgt tcccgcgcat cggacgaatt
     3001 ggcacggatt ttcggcacgc aggcagacat cggcagccgt gccgccgaca tcggacacgg
     3061 catcaaacaa atcggcagga acatcgccga acaaatcggc agattttccc gcgaacccga
     3121 gtccgcaaac atcggcaacg aagcccttgc cgactgcctc gacgaaataa gcagactgcg
     3181 cgacggcgtg gaacgcctca acgaacgcct cgaccggctc gaacgcgaca tttggataga
     3241 ctaaccttca gacggcatcc gacatgaaca gcccttttca caacatcggc atcgtaaccc
     3301 gccccaacac gcccgacatc caagacaccg cacacacgct gattaccttt ttgaagcagc
     3361 acggctttac cgtctatctc gacgaagtcg gcataaagga aggctgcatc tatacccaag
     3421 acaccgtcgg ctgccatatc gtcaacaaga ccgaactggg gcaatactgc gacctggtcg
     3481 ccgttttagg cggagacggc acctttctct ccgtcgcccg cgaaatcgcc ctgcgcgccg
     3541 ttccgattat cggcatcaac caagggcatt tgggcttcct gacccaaatt ccccgcgaat
     3601 atatgacgga caagctattg cccgttttag aagggaaata ccttgccgaa gagcgcatcc
     3661 tgattgaggc cgcactcatc cgcgaaggca aaaccgccga acgcgccatc gccctcaacg
     3721 atgccgtcct ctcccgtggc ggtgccggac agatgattga atttgaagtc ttcgtcaatc
     3781 gggaattcgt ctatacccag cgttcggacg ggctgattgt ctccaccccc accggatcga
     3841 ccgcctattc gcttgccgcc ggcggcccca tcatgcaggc aggtttgcac gccttcacgc
     3901 tcgtccccat ctgcccgcaa tccatgacca accgccccat cgccattcca gacacgtccg
     3961 aaatcgaaat cctcgttacc caaggcggcg acgcgcgcgt ccatttcgac ggtcaaaccc
     4021 atatcgacgt gcaaaacctc gaccgcatca ccatccgccg ctaccgaaac cccttacgca
     4081 tcctacatcc gaccgactac caatatttca aaaccctgcg ccaaaaactg cactggggcg
     4141 aacaattggt ctgaaccgac ccttaccgga aaacacctat gaatccaaaa aacccccccg
     4201 accatacaga agatgccgtg cgcctctcca aacgcatggc gcaactgggg ctttgttcgc
     4261 gccgcgaagc cgacggctac atcggacagg gctgggtaac ggtcaacggc aaaaccgccg
     4321 tactcggtca gaaagtttca ccggccgacc gtatcgaact aaacaagaaa gcccacgaac
     4381 agcaggcggc acgcattacc atcctgttga acaaacctgt cggctatgtc agcgcacaag
     4441 cggaaaaagg ctataaatcc gccgccgaac tgattacccc tgaaaaccgc tgggaaggcg
     4501 acaccggacg catccgtttc gatccgaaac acaaaatcgg tcttgccccc gccggcagac
     4561 tcgatatcga ttcagtcgga ttgctggtgt tgacgcaaga tggccgtatc gccaagcagc
     4621 ttatcggcga aaacagcggc agtgaaaaag aatatttggt gcgcgtgcgc ggcaaattgg
     4681 acgaaaaagg acttgcctta ctgaatcacg gattgagttt ggacggcgag aaactgcgtc
     4741 ccgccaaagt agaatggcaa aacgaagacc aactgcgctt cgtgttgaaa cagggtaaaa
     4801 agcggcaaat ccgccgtatg tgcgaactgg tcggactgcg cgtcgtcggg ctgaaacgca
     4861 tccgcatggg caaggtcaaa ctcggcaggc tgccgcccgg caaatggcgt tatctcgccc
     4921 ccggtgaatc gttttaaata agcattaaaa aatgccgtct gaaaatcctt gcgttttcag
     4981 acggcattgc cttatgccca aataacggtt tcttccaagg gcctacgcgc tttcgtggcg
     5041 atttcctgaa cgcggtagcc gaatgtcgcg gcgacggaca cgccccattc tgccgcatcg
     5101 aacaaaccaa actgcccgga caatatgcgc tccatctcgg catagttgaa accttccacc
     5161 gggcaggaat cgatacctgc catcgccgca cccgtcatca tgttggctaa cgcgatatag
     5221 gtctgacggc aacaccagtc aaacaaggcg cgagaatcgt ccaaaatctt gatgtcgtca
     5281 gcttgaaacg cctgatacct tgccaaagat tttgctacgg catccggttc ggtaacgccg
     5341 cgccgtttga ggctttccaa cataaacggg ctgtcggagc gggcattttt cttcgccaaa
     5401 aacaccacca aatgactggc ggtatccaaa gcatccgcca taccccaaga aaacggcttg
     5461 attgcctgtc ggatttcagg gttttgaacc acaataaact gccaaggctc cgaaccgacc
     5521 gaactgggcg acaaacgccc gagttctaaa ataaactgaa aatcctcggc actgattttg
     5581 cgtgccgcat cgtaatgccg gcatgattta cggtttttaa atgcggatag aacctgctct
     5641 ttgcttaata ctgtcatcgt gcatcctttt attacttgta ttgcaaattg atttccgata
     5701 cggcggcaga ttcgccaata acgcccaaac gcctttcaga cggcatttgg ttcatcgcaa
     5761 acacgcctac ggatacgcga tataggcata agccgaatgg ttgtggatag attcgaaatt
     5821 ctcactctcg acgacaaagc tcttgatacg cttgtcggca atcagtgcag tagcgacatc
     5881 acgcaccata tcttccacga atttcggatt ttcgtaggcc ttttcggtaa cgtatttttc
     5941 atcggggcgt ttgagcaggc cgtagagttg gcagctcgcc tgcgtttcca cataatcgat
     6001 aacttcctcg ataccgactt cggcatcggc agtcaggctg acggtaacgt gcgaacgctg
     6061 gttgtgcgcg ccatattggg aaatttcttt ggaacacggg caaagcgagg ttacgggaat
     6121 catgaccttc atactgtggc cgtatgcccc gtctttgatt tcgcccgtga gacagacatc
     6181 ataatccagt aaggaccgga taccggaaac cggcgcggtt ttcttgcgga aaaacggaaa
     6241 agaaacgctg attttgccgg cgcgggaatc caaaagcgcg accatttcgg cggtcagcct
     6301 atgcaattgt gcaaaatcca aagcttcggt atgttgctcc atcaatgcga caaagcgcga
     6361 catatgcgta cccttctgct cggcgggcag gtagaccgtc atggtcaggc gggcaaccgt
     6421 ggattgcgtt ccttcggcgg tttgcagggt aatcgggaag cgcaggtctt taatgccgac
     6481 ctgattgatc ggcaggttgc gtaaatctct gctggattgc acgtctgcaa tggcactcat
     6541 gcgttgtttt tccttaatat tcagataatt attgagatgt gtgtattgta tggcaggcag
     6601 atgccgtctg aaaaaacgct gttgaccgcc tgcctgcaaa tgcgagatta tatcacttgc
     6661 ttttggcggc tgcattgatt gtttctattt tcgggatagt gataaaatcc cgacttattc
     6721 atatcttaca aggcagaaac agatgaaatt cgccacccaa gccatccatt ccggctacga
     6781 ttgcgacgaa cacaaccgtg cgctgatgcc gccgatttat caaaacagta tgtttgcgtt
     6841 gcacgagatt ggcgaaaatg tgccttaccg ttattcgcgc ctgagcaacc cgacccgtca
     6901 gattttggaa gacaccgttg ccgatttgga acacggtgcg gcaggttttg tgttttccag
     6961 cggtatggcg ggaattgatg ccgtgtggcg taccttcctg cgccctggcg atactattgt
     7021 cgccgtcgct gatatttacg gcggcgcgta tgacttattg gtcgatgttt atcaaaaatg
     7081 gggtgtaaac gttgtttttg ccgatttggg caatccggat aatctggacg agctacttaa
     7141 agcgcacaag gtcaaactgg tttggctgga aacgccgtcc aatccgcttt tacgcttggt
     7201 agacatcaaa gcccttgccg caaaagccaa agcggccggt gcgctggtgg gtatcgacaa
     7261 cacctttgcc acgccgtatc tgcaacagcc cttggatatg ggttgcgatt ttgtgttcca
     7321 ctctgctacc aaatatttgt gcggccattc cgacgtattg atgggcatcg tcgttgccaa
     7381 aaccaaagaa ctggcgcagc ctttgcacga tatgatggtg cataccggcg cggttgccgg
     7441 cccgctggac tgctggctgg tgttgcgcgg catcaaaacg cttgccctgc gtatggaggc
     7501 acactgcaaa aacgcactcg aaatcgcgcg ccgtctggaa gaccatcctg ccattgaaaa
     7561 agtgttccat cccggcctgc cgtcgcacga acattatgaa ctcgcccaag cgcaaatgcc
     7621 taaaggcatc ggcggcgtgg tgacggttta tctcaaaaac gacacgcgcg aagcggcaaa
     7681 cagcgtgatt aaaaacatga aactggtcaa aatggcttcc agcctcggcg gcgtggaaag
     7741 tttggtcaac cattgctatt cccagtccca cagcggcgtg ccgcataatg tgaaaatgga
     7801 aatgggcatc agggtcggtc tgctgcgttt ctctgtcggt atcgaagatg tagatgatat
     7861 ttggaatgat atttccgccg cactggatac gactgtataa tccacgaaaa atgccgtctg
     7921 aaaccatggt ttcagacggc atttcaatca aaccgaccga aaatcaacgc ttcagcatct
     7981 ttgccgcctc aatggcataa taggtcaaaa tcccgtccgc gcccgcacgt ttgaatgcca
     8041 gcaggctttc caagaccacc ttatcgccgt ccagccagcc gttggcaacc gccgcctgca
     8101 gcatcgcata ttcgcccgaa acctgatagg cataagtcgg cacaccgaac tcgtccttca
     8161 cgcgtcggac aacgtccaaa tacggcaaac cgggcttgac catcaccata tccgcacctt
     8221 cctgaatgtc caacgccacc tcatgcagcg cctcatcggt atttgcggga tccatctgat
     8281 aggtcttttt atctgccttg cccaaattgc ccgaactgcc taccgcatca cggaaagggc
     8341 cgtaaaatgc agaagcatat ttggcggaat acgccataat ccgcgtatgg atatgcccgg
     8401 catcctccaa cgcctcgcga atcgcaccga tacgcccgtc catcatatcg gaaggggcaa
     8461 ccacctgcgc gcccgcttca gcgtggcaca aagcctgctt gaccaaaacc tctacggttt
     8521 catcgttcat cacataaccg ttttcgtccg tcagcccgtc ctgaccgtga accgtataag
     8581 gatcgagcgc gacatccgtc ataatgccca gttcgggaaa cctctcgcgc aaggcgcgga
     8641 cagttgacgg cacgagtcct tcgggattgt acgcctcctg cgcacgctcg gttttgtttg
     8701 ccgtaaccac ggggaacagt gccaacatcg gaataccgag ctttaccgcc tcttccgccg
     8761 taaacagcag cctgtccaaa ctttgacgct tcacacccgg catagaaggc acatcctcct
     8821 cgcgcgccga cccctccaat acgaacaccg gataaatcaa atcatcggcg gtcagcgtgt
     8881 gttcgcgcat caggcggcgt gaaaaatcgt ccctgcgcat acggcgcata cgcgaagccg
     8941 gaacattgcg gtaaggaaac tgcataagcc ctccaatcat cctgtcaaca attcaaacca
     9001 tacggaagcc gccgcaactt gcagacagca tcgggcggca atcaaaatcc gttttcaccg
     9061 gaaagggttt cggcccgccg aaatgccgcg ccgaaggacg gcgtcccgcc cattaaagaa
     9121 caatagcaaa acaacagctt caggctgtca gccttcgata ttcttacgcc acaataccaa
     9181 aagtttcccg atatgctgaa caggttgcgc gtcaaccgcc tcacataagg cagtgcagat
     9241 ttcgatacgt tcggcacggt cgtcgccgaa tacgcgcact ttaatcagct catgcgccgt
     9301 caatgccgca tcggtttcct tgatgaccgc gtccgtcaga ccctgctgac cgaccatcac
     9361 aacaggatgg agatggtgcg cgcgcgcttt cagttccaaa atttctttgg tgttcaattt
     9421 ggtatccgtc attttcctac tgcttgaata aaaagaataa tgcggcattg tacgcgattt
     9481 ggcacgacat cggcaaaatt aacgtacaat acgcggtttc cattccgacc cgcgaaccac
     9541 tatggctgta cgttccaaat cctcaaaagc gtggctgcac gaacacgtca acgaccacta
     9601 cgtccatatg gcgcaaaaag acggctaccg cgcccgtgcc gcatacaaac ttttggaaat
     9661 caacgaaaaa gacaaattaa tcaaacccgg cacggtactt gccgacttgg gcagcgcacc
     9721 gggaagctgg tcgcaggttg ccgccaagct gacgggtact tccggcgcgg tattcgcttt
     9781 ggacatccta cccatggaag ccataggggg cgtctccttc attcagggcg acttccgcga
     9841 gaacgacgta ctggcacaat ttgaaacctt gttggacaac cgcccgctcg accttgtaat
     9901 ttgcgatatg gcgcccaata tgtcgggaaa cgccgtaagc gatcaggcac gcagctttta
     9961 tctgtgcgaa ctggctttag acttcgcctc gcaacacctg aaaaccggcg gcagcttttt
    10021 ggtcaaagtc tttcagggtg caggctatca ggaatacatg gcagccatgc gcgaaatttt
    10081 cggcacggtg cagacgcgca aacccgaagc ctcgcgcaat cgctccagtg agatttattt
    10141 attgggcaaa aataaacgct gacaatacag acggcgtgct ttacaatcat ttccgtttta
    10201 cacctcaatt atggagcctt gctaagtggg gaacaccttt aaatcaatcc ttgtctgggt
    10261 cgccttgggt atcggcctga tggctgcatt caacgcttta gacggtaaaa aagaagacaa
    10321 cgggcaaatc gaatattctc agttcatcca acaggtcaac aacggcgaag tatccggcgt
    10381 caacatcgaa ggatccgtcg tcagcggcta cctgattaag ggcgagcgca ccgacaaaag
    10441 caccttcttt accaacgcgc ctttggacga caacctgatt aaaacactgc tcgacaaaaa
    10501 cgtccgcgta aaagtaacgc cggaagaaaa accgagcgcg ctggctgccc tgttttacag
    10561 cctgctgccc gtcctgctgc tgattggcgc gtggttctac tttatgcgta tgcagacggg
    10621 cggcggcgga aaaggcggcg cattctcatt cggcaaaagc cgcgcccgcc tactggacaa
    10681 agatgccaac aaagtgacct ttgccgatgt cgccggctgc gacgaagcca aagaagaagt
    10741 gcaggaaatc gtcgattacc tcaaagcgcc gaaccgctat caaagcctgg gcgggcgcgt
    10801 gccgcgcggc atcctgctgg cgggcagccc gggtacgggt aagacgcttt tggcgaaagc
    10861 gattgcaggc gaagccggcg tgccgttctt cagcatttca ggttccgact ttgtcgaaat
    10921 gttcgtcggt gtcggtgcga gccgcgtccg cgatatgttc gagcaggcga agaaaaacgc
    10981 cccctgcatc atctttatcg acgagattga cgcagtcggc cgccaacgcg gcgcaggttt
    11041 gggcggcggc aatgatgagc gcgagcaaac attaaaccaa ttgttggttg aaatggacgg
    11101 ttttgagagc aatcagactg taattgtgat tgcggcaacc aaccgtcccg acgtactcga
    11161 tcctgcgctg caacgccccg gccgtttcga ccgccaagtg gttgtccccc tgccggacat
    11221 ccgagggcgc gaacagattt tgaacgtcca ttctaaaaaa gtgcctttgg acgaatctgt
    11281 ggatttattg tccctcgcgc gcggcacgcc gggtttttcc ggcgcggatt tggcgaactt
    11341 ggtcaacgaa gccgccctgt ttgccggccg ccgcaataaa gtcaaagtcg atcagagcga
    11401 ttttgaagac gccaaagaca aaatctatat gggtccggaa cgccgcagta tggtgatgca
    11461 cgaagacgaa aaacgtgcga cggcgtatca cgaatccgga cacgcgattg ttgccgaaag
    11521 cctgcccttt accgaccccg tccacaaagt aaccattatg ccgcgcggac gtgcgctggg
    11581 tctgacttgg cagcttccgg agcgcgaccg catcagtatg tataaagatc agatgttgag
    11641 ccagctctcc atcctgttcg gcggacggat tgccgaagac atcttcgtcg gacgcatctc
    11701 caccggcgca tcaaacgact ttgaacgcgc aacccaaatg gcgcgcgaaa tggtaacgcg
    11761 ctacggcatg agcgacaaaa tgggcgtgat ggtttatgcg gaaaacgaag gcgaagtctt
    11821 cttgggacgc agcgtaaccc gttcgcaaaa catttccgag aaaacccagc aggacatcga
    11881 cgcggaaatc cgccggattt tggacgagca atatcaggtt gcctacaaaa tcctcgatga
    11941 aaaccgcgac aagatggaaa cgatgtgcaa agccctgatg gaatgggaaa ccatcgaccg
    12001 cgatcaggta ctggaaatca tggcgggcaa acaacccagc ccgcccaaag attacagcca
    12061 caacctgcgc gagaatgcgg acgcggcgga agataacgcg ccgcacgctc cgactcggga
    12121 agaaaccgaa gcacctgccc cggcagacac cgcttcgtca gagtccgagc agcggtctga
    12181 aaacaaggct taacttcccg aacaaacggc agcccgcaag ctgccgtttt cccatcctgt
    12241 tttcagacgg cattttccgc tcaatgccgt ctgaaacgcc aaagccgtat cggtttttgc
    12301 gaatctgcga tatggcggtt taaattgaaa tcagaacggg gcaaggctgt accggtttaa
    12361 aattaaaccg ctataatacc gcccttcgaa tacacgcccg cattggtgat gcccgtgaaa
    12421 aaagttgaaa aaaacatctt ggtgctgcac ggcgcggaca aaatgttcga gctggtcgat
    12481 aaggttgagg attacccgaa ctttctgccg tggtacagca aaaccgaggt catcggacgc
    12541 aacggcaacg aactgaaggc gcggctgttt atggactata tgcacgttcg ccaatcattc
    12601 gccacgcaca accgcaacat tccgggcagg gaaatccgta tggaactgct cgaaggtccg
    12661 ttcaaaacct tacgtggaac gtggaaattt atcgatttgg gcgacgatat gtgcaaaatc
    12721 gaattcaatt tggaatacga tttttccaat gccgttttgt ccgccttaat ttcccccgtc
    12781 ttcaaccacc tttccgccac gctggtcgaa gcgttcgtca aagaggcaga ccgccgttat
    12841 gcttgaaatt gagattgtgt acgggctgcc cgaccgacag gttttgaaaa ccatgcagct
    12901 tgccgaggga acaaccgtcc gcgccgccgc actgcaaagc ggtttggacg gcatatttga
    12961 agatttaaac ctgcattccg cgcctttggg cattttcggc aaagccgtca aagacgacac
    13021 gccgctgcgc gacggcgacc gcatcgaagt gtaccgcccg ctgttgatcg accccaaaga
    13081 agcgcgccgc aaacgcgttc aaaatcaaga agaataacca tgccgtctga agccttcaga
    13141 cggcattcgg cagaaacacg gaaaatatca tgtcaaacac aatcaaaatg gttgtcggct
    13201 tgggcaaccc gggcaaagaa tacgaacaga cacgccacaa tgcgggtttt tggttcctcg
    13261 acgaactggc gtggaaatgg aaggcttcat ttaaagaaga aaaaaaattc ttcggcgaag
    13321 tcgcccgtgc taccctgccc gacggcgatg tctggctgct caagccgacc acgttcatga
    13381 accgttccgg acaggcagtt gccgcccttg cgcagtttta taaaatcaaa cccgaagaaa
    13441 tcctcgtcgt ccacgacgaa ctcgacattc cctgcggacg gatcaaattc aaactcggcg
    13501 gcggcaacgg tggacacaac ggcttgaaag acattcaggc aaaactcggc acggcagact
    13561 attaccgcct gcgcctcggc atcggccacc cgggcgaccg caacctcgtc gtcggctatg
    13621 tcctgaacaa acccagtacg gaacaccgcc gacagattga cgatgccgtc gccaaatccc
    13681 tgcaagccat acccgacatc cttgccgaca aatgtgaaga ggcaacccgc ttcctgcaca
    13741 gcaaatgacc cgatgccgtc tgaagccctt tcagacggca tgttcccgat ttccatatcc
    13801 gaacagtcat gaccgaactc aagcagctta tccaaaccga atccatcccc gtcatcgaag
    13861 aaaccctcga tttcctgctg tacgaatgca gcatcgacga cgcaccatcc gccgaagaag
    13921 tggcacaatg gcgcgacata cttgccgcac gcggcggcaa attcctgcgc ctgtccaaaa
    13981 tctgccaaac gtggctggac gaggaggcgg catgaagctg ccgcgcaacc gcttcagcct
    14041 gctttccgca ttgtggtttg ccggcggcat ctattcgctg ctcttcaaag ctgccgacac
    14101 cgcgccgccg ccgtttccgc atttcgacaa agcagcacac cttgccctgt ttttcgcaca
    14161 aatctggctt ttgaccaaag cattcaaaac cggaaaactt cccatcccct accgcagcct
    14221 gatggtcttt gccctctgtt tcgccctctt cagcgaatgc gcgcaggcat ggtttaccgc
    14281 aacgagaacc ggcagtttgg gcgatgttct tgccgatatg gcaggtacgg ttctcgcact
    14341 ctttgccgcc cgcgccgccg accgcccgga ctaaatcggt ttatttcccc caaacaggat
    14401 gcacctcttc ccgatacatc ctatttttcc ccttacatat caatcccatc tcataaacaa
    14461 aaccgacaaa tcgtttacaa tatatttaca ctacatcaga ttacaaatat actcgaacca
    14521 gttgcagaag cggcgattcc acaaaccgtt tcggattgca cgacacaaaa gaaaaaccga
    14581 ttttattgcc ttaaaggagt attcatgaac ctgcatgcaa aggacaaaac ccagcatccc
    14641 gaaaacgtcg agctgctcag tgcgcagaag ccgattaccg actttaaggg cctgctgacc
    14701 accattattt ccgccgtcgt ctgtttcggc atttaccaca tcctgcctta cagccccgat
    14761 gccaataaag gtatcgcgct gctgattttc gttgccgcac tttggtttac cgaagccgtc
    14821 cacattaccg taaccgcact gatggtgccg attctcgccg tcgtactcgg tttccccgac
    14881 atggacatca aaaaggcgat ggctgatttt tccaacccga ttatctacat ttttttcgga
    14941 ggcttcgcgc ttgccaccgc cctgcatatg cagcggctgg accgtaaaat cgccgtcagc
    15001 ctgttgcgcc tgtcgcgcgg caatatgaaa gtggcggttt tgatgttgtt cctcgttacc
    15061 gcctttctgt ccatgtggat cagcaacacc gccaccgccg cgatgatgct gcctctagca
    15121 atgggtatgc tgagccacct cgaccaggaa aaagaacaca aaacctacgt cttcctcctg
    15181 ctcggcatcg cctattgcgc cagcatcggc ggcttgggca cgctcgtcgg ctcgccgccc
    15241 aacctgattg ccgccaaagc cctaaatctg gacttcgtcg gctggatgaa gctcggcctg
    15301 ccgatgatgc tgttgattct gcccttgatg ctgctctccc tgtacgtcat cctcaaacct
    15361 aatttgaacg aacgcgtgga aatcaaagcc gaatccatcc cttggacgct gcaccgcgtg
    15421 atcgcgctgt tgattttcct tgccacagcc gccgcgtgga tattcagctc caaaatcaaa
    15481 atcgccttcg gcatttccaa tcccgacacc gttatcgccc tgagtgccgc cgtcgccgtc
    15541 gtcgtcttcg gcgtggcgca atggaaggaa gtcgcccgca ataccgactg gggcgtgttg
    15601 atgctcttcg gcggcggcat cagcctgagc acgctgttga aaacatccgg cgcgtccgaa
    15661 gccttgggac agcaggttgc cgccaccttt tccggcgcgc ccgcattttt ggtgatactc
    15721 atcgtcgccg ccttcattat ttttctgacc gagttcacca gcaacaccgc ctccgccgca
    15781 ttgcttgtac cgattttctc cggcatcgct atgcagatgg ggctgcccga acaagtcttg
    15841 gtattcgtca tcggcatcgg cgcatcttgt gccttcatgc tgccggttgc cacaccgcct
    15901 aacgcgattg tgttcggcac gggcttaatc aagcaacgcg aaatgatgaa tgtcggcata
    15961 ctgctgaaca tcctctgcgt agtattggtt gctctgtggg cttatgctgt actgatgtaa
    16021 accatcgacc taaacaacaa gaccgtctga aagaatattt ttcagacgac cttgaagttt
    16081 gtcgttcaga cacaatttgt cgaatcattc aaaaccggat tctaacgaaa ggaaacccat
    16141 gattatcctg cacaccaaca aaggcgacat caaaatcgaa ctcgatttcg acaaagcccc
    16201 cgttactgct aaaaacttcg agcaatacgt taaagacggc ttctacgacg gcgtaatctt
    16261 ccaccgcgtt atcaaaggct tcatgattca aggcggcggc atggatgaaa acatgaacga
    16321 aaaagaaacc cgcgatccga ttcaaaacga agcgtccaac ggcctgccca acgataaata
    16381 caccatcgcc atggcacgca cttccgaccc ccattccgcc agcgcgcaat tcttcatcaa
    16441 cactgccgac aacgctttcc tgaacttccg ttctaaagag ctgtacggca aaaccgtcgt
    16501 ccaagactgg ggctatgcag tattcggtaa agtcgttgac ggttttgacg ttgtcgatgc
    16561 catcgaaggc gtatccacca aacgccacgg ctaccatgac gacgtaccga gcgaacctgt
    16621 cgtcatcatt aaagccgaag cggtataaac cggcaatccg aaagcagcct gcacaaaggc
    16681 tgcttttttt gtttcaggcg gcataaaatc caagcacata tgccgtctga acacctcccg
    16741 caagccttcg gacaatgttg caacgtgtta aaatagcctc ctttcctgaa accacaacaa
    16801 ggaaaccatc atggcaaata ttgcccgcga catcttcaaa gcctacgaca tccgaggcat
    16861 tgtcggcaaa accctgactg acgatgccgc ctatctcatc ggcagggcca tcgccgccaa
    16921 agccgccgaa aaaggcatga cccgcatcgc gctcggacgc gacggacgct tgagcggtcc
    16981 cgaactgatg gagcacatcc aacgcggctt taccgacagc ggtatcggcg tcctcaacgt
    17041 cggtatggtc gccacgccca tgctctattt tgccgctatc aacgaatgcg gcggcagcgg
    17101 cgtgatgatt accggcagcc acaatccgcc tgattacaac ggtttcaaaa tgatgctcgg
    17161 cggcgacacg cttgccggcg aagccatcca agagcttttg tccatcattg aaaaagacgg
    17221 ttttgctgcc gccggcaaac aaggcagcgt aaccgaaaaa gacatctccg gcgaatacca
    17281 caaccacatc gtcggacacg tcaaactcaa acgcccgatg aacatcgcca ttgacgcggg
    17341 caacggcgtg ggcggcgcat ttgcaggcaa actctaccgc gctttgaggt gcggcgttac
    17401 cgaacttttc tgcgacgtgg acggcaattt ccccaaccac catcccgacc catccaaacc
    17461 caaaaacctg caagatttga ttgccgcgct gaaaaacggc gatgccgaaa tcggcttggc
    17521 gtttgacggc gatgccgacc gcttgggcgt ggttaccaaa gacggcaaca tcatctatcc
    17581 ggaccgccaa ctgatgcttt tcgcgcaaga cgtgttgagc cgcaatccta aagccaaagt
    17641 gattttcgac gtcaaatcca cccgtctgct cgccccgtgg gttaaagaac acggcggcga
    17701 acctgtgatg gaaaaaaccg gccacagctt catcaaatcc gctatgaaaa aaaccggcgc
    17761 actcatcgcc ggcgaaatga gcggacacat cttcttcaaa gaacgctggt tcggcttcga
    17821 cgacggtctg tacgccggcg cgcgcctctt ggaaatcctg tctgctttcg acaatccgtc
    17881 cgaagtgtta aacaacctgc cgcaaagcat ttccacgccc gaactcaaca tcgatctgcc
    17941 cgaaggcggc aacgggcata aagtgattga agaactcgcc gccaaagcca agttcgaagg
    18001 cgcgaccgaa atcatcacca tcgacggctt gcgcgttgaa ttccctgacg gcttcggcct
    18061 gatgcgtgct tccaatacca cgccgatttt ggtattgcgt tttgaagcgg atacgcaaga
    18121 agccatcgag cggattcaaa accaattcaa agccgtcatc gaaagcaatc cgaatctaat
    18181 ctggcctctg taaacatcga aaaaatgccg tctgaaggct tcagacggca tttttcacaa
    18241 atggccaatc aaatcttggc agattggatt tggcttaaaa atttccgcgt ccgttcgtgt
    18301 tgggggtggt cgaacaactc tttcgggctg ccctgctcta cgataacgcc gccgtccatc
    18361 acgacaacgg tcgtggcaac ttccagcgcg aacttgattt cgtgggtaac gacgaccatc
    18421 gtccaacctt cccgcgccaa ttccttcatg gcgttcaaca cgtcttgcac caactcgggg
    18481 tcaagcgcgg aagtgggttc gtcaaacaac atcagctcgg gctgaatcgc caatgctcgg
    18541 gcaatgccga cgcgctgctg ctgaccgccg gaaagctggt agggatacaa atccactttg
    18601 tcgcccaagc cgactttttc cagcagtttc agagcctctt cgcgcgcttg ggcggcaggc
    18661 ttgccctgta cggcaaccgg tccttccatc acgttttcca aggcggtttt gtgcggaaag
    18721 aggttgtatt gttgaaacac catgcctgat ttgcggcgca gtgctaaaat atcgtgtttg
    18781 cttggttttt tagaaaaatc gattttcagc ggtcgctcgt tgtcgaactc gatttgtccg
    18841 tcttcgggca tttccaacgc gtttaggcag cgcagaaacg tcgttttgcc tgagccggaa
    18901 ggcccgagga tgacgaccac ctgccctttg cacacatcca aatcgatgcc gcgcaaaatg
    18961 gtattttcgc cgaaggtctt atggatattg cggattttaa tcatgacaac tccttatttg
    19021 gcgacgtagc ggtcgaaacg tttttccaaa cgcgcctgaa tcaggaacag cactttacaa
    19081 aaacaccagt aaaccaaagc ggcttcgata tagacgggca aaaagtcata agtgcggttt
    19141 gccgtttcct gcgcgacgcg gaataattcc gttaccgtca cgactgccgc gagcgaggtg
    19201 tttttaaaca aaccgataaa ctcgttgctc aaaggcggca cggcaacgcg gaatgcctgc
    19261 ggcgcgacaa tgcggcggaa cgtctgcata taggtcatgc cgatggagaa acctgcttcc
    19321 cattggcctt tcggtacgga caaaattgcc gcgcgtatgg tttcggaagc atatgcgccg
    19381 acattgagcg aaaagccgat gatggcggca ggaatcgggt cgatatagat gccgacggaa
    19441 ggcagcccgt aaaacacaat cacaagctga accaacagcg gcgtaccgcg aatgacggaa
    19501 atataaaatt ccaccaattt cagcaggatt ttccgcacga tgccgccggc gggcataatc
    19561 cgcaccaaag ccatggctac cgcaatcatc ataccgataa cgaaagaagc tgccgccaaa
    19621 ggcagagaga ccgcgaagcc ggctttgacc ataggcaaaa acgcgctgac aatcatatcg
    19681 gcgcgtgttt ccgtcataaa cggcagcgaa gcaaggaaat tattgaacac tgatgtcttt
    19741 tccgaagaat tgttcgccca gttttttcag cgtaccgtcg gctttcagct cgttgattgc
    19801 cgtactgaat ttcgccacgg cttcgtcatt gcccttgttg acaatcagac cggaaccgac
    19861 tttttcatcg gcaggtgcgg accaaacgat tttcacgccc gcattcgggt ttttcttcag
    19921 atagtccaaa accgccaatt cgtcgttcag ggttgcatcg gcacgttttt gttcaatcag
    19981 ggtcagcgat tgcgccaaac cgtcaacagc caccaaatct gcgcctgcag ctttggcttt
    20041 ttcgccgtag ttgctggtca gggattgtgc ggttttcacg cctttgatgt cgtcgataga
    20101 tttgatgttg ctgtcgttac gggcaaccaa tacggcaccg ctccagctgt aaggatcgga
    20161 tttgtcgaat gtcgcttggc gttcggggct ggtcagaccg acttggtttg ccaccacgtc
    20221 gaaacgcccc gccttcaaac ccgccatcat cgaatcccat tgcgtttctt taaactcgac
    20281 tttcacgccc agtttttccg ccacggcgcg ggttacttcc acatcgtaac cggtcagttt
    20341 gccgtccttg tcgtggtagg taaacggtgc gtaagtgcct tccgtgccga cggtaaccgt
    20401 gcctttattg ttgatgcgct cgattaaaga accggaaact gccgattgtg caggcgcgga
    20461 agatgctccg ctgccgcctt ccgaaccgcc gcaggccgcc aaaaccaatg cggcaatgcc
    20521 gccgagtacg aattttttca acatcattct ctcctgtaaa aagtgtgcaa gtgcggcatt
    20581 ctaagtgaaa ttgcagcaaa cacaaagaat acttttttta tatggttatt ccaaaccgga
    20641 atggaaaaga tataccgaag ccgccggaag cggaaataaa gggggcggga aaggcggatt
    20701 cccgcacgct gttccggcaa aaatccgcct gccgattcgg tgctataatc cacccgaaac
    20761 agatgacacc aaaaaggaac atcatgaaca cgccccaatc cgccattatt cccgaccacg
    20821 cccaagccgg catctttatc gaagccgact tcgccgccaa ccgcctcaac gacattaaag
    20881 ccgcctgccg cgcttcgctt gacgcgttat ccgccttgaa aacccgcttt ccggacgata
    20941 ttttgggtct gaccatcgcc ttcggcagca aagcctgggc aacattcgga catacggacg
    21001 aaggcagtga aatcaaaccc ttctccgaaa tgggcaatgg gcttgcgccg tccacgcagc
    21061 acgatatgta catccacatc caatccttcc gccaaaacgc cgcctacgcg cttgcccaat
    21121 ccgttttggg cgcattcggc gacagcatat gcgtcgcgtc cgaagaacac ggtttgcgcc
    21181 tgtatcagga tcgcggactg gacggtttcg tcgatagcac ggaaaacccg cagggcgatg
    21241 aaaaaatccg cgaagtcgcc atcatccccg aaggactgcc cgatgcgggc ggcagctatg
    21301 tcctgctgca aaaatacctg cacgatttga aaaaatggga tgccgtcccc gtcgccgaac
    21361 aggaggcctc ggtcggacgc agcaaggaaa ccgacgacga attcagccgc gatgtccgcc
    21421 tgcctgattc gcacctcggc cgcgtcaacc tgaaagaaaa cggagtcggc ctgaaaatcg
    21481 tccgccgcag cctgcccttc ggcaaaatca gcggcgaaca cggtttgatg tttaccgcct
    21541 actgccgcac gctgcacaat atcgaagcgc aattattgag tatgttcggc gacacggatg
    21601 gcaaaaccga cctgctgctc cggcacctct ccgccgccgt ttcgggcgga tactactacg
    21661 cgccgtctgt cgaacgcctg caaaacctct aagccggacg atgccgtctg aaaccctttt
    21721 tcagacggca ttttcccgtt ttaccgtcca accgttttcc gagcgcgcac tatgtctgca
    21781 agcatccaag cattcgaccc gctaaccgtc cccatttccg gcaccaacct gattgaggcc
    21841 tctgccggca ccggcaaaac ctacggcatt gccgccctgt ttacacgttt gatcgtatta
    21901 gaacaaaaaa gcgtcgaacg cgtattggtc gttaccttca ccaaagccgc caccgccgag
    21961 ttgaaaacac gcctgcgcgc gcgtttggac gatgtgttgc aggttttgga aagcaaagaa
    22021 attgccaaac ttggagacga cacgctttca gacggcattg ccgcctactg cgccgaacac
    22081 cacgaaggcg acaccttcct gcccgcactc ttaaaacagg ctttgcaaaa agagggccgg
    22141 acgcgtctga ttgtccgcct caaggccgcc atcgggcaat tcgacaacgc cgccatctac
    22201 accatccacg gcttctgcca acgtatcctg cgcgactacg ccttcctgtg ccaagcaccg
    22261 ttcgatgtcg aactgaccga agaagacggc gaccgcctgc ttatcccggc gcaagatttt
    22321 tggcgggaac gcgtcagcaa tgatccggtg cttgccgcat tggcgtttaa acgcaaagct
    22381 gtgccgcaaa ccgtccttgc ccaaatccgc gcctacctgt cccgcccgta cctgaatttc
    22441 cgccgtccgc aggcggattt gaaacaggcg cagcgcaacg ccgaaacctc atggcaaacc
    22501 gtctgccgcc tgctgcccga gctggaagcc ggcttttggc gcatccatcc cgacctcaac
    22561 ggcaacagtt accgcaaaaa cagcttcggc aaccttttta aagaactggc acaaaaatcc
    22621 gccgccggac aactgccctt tctggacaaa gacacgcacg acagactctt gaaacttgcg
    22681 tccgacaaac tcgaagccgg actgaaaaaa ggcaaaacgc ccgatgcggc agtatttgcc
    22741 gaattgcaga aactggtaga cttcgggtac gatttgaacg cactcgaaga agcggaagaa
    22801 gcaacaataa tccggctgca actggattta atcggatacc tcaaccgcag ccttgccgag
    22861 atgaaaaaat cgcgccgcga acgcggtttc gacgacctgc tgctcgatgt ccacaccgcg
    22921 ctgaccgaca atccgcacgc cgaaaccctc gcccgcgccg ttgccgaaaa ctgggaaacc
    22981 gcgctgatcg acgagttcca agacaccgac ccgctgcaat acgaaatctt ccaaaagatt
    23041 ttcatcgccc aaaaccgacc gctgttcctc gtcggcgatc ccaaacaggc gatttacagc
    23101 tttcgcggag cggacattta cgcctacctt caggcggcgg aagacgcgcg gcaccgctac
    23161 acgctcgcca ccaactaccg cagccacgcc gcgcttatcg gcagcatagg cgcgctgttc
    23221 cgcctcaaag aacgcccgtt cgttttggaa aacatcggct attcggaagt cggtgcggcg
    23281 cgtgccgaaa gcaggctgtc ccccgaacgt cctgccgtac aggttcgttg gctgcacgaa
    23341 aacgacaatg aaaaagccaa caaagacgtt ttgcgccgcc gtgccgccga ctattgcgcc
    23401 gacgaaatcg cccacgcgct caacgaagcc gccagaggcc gtctgaattt caaaggctgc
    23461 ccgttgcagt cgggcgatat tgccgtgctg gtacgcacgc acaacgaggc ggtgatggtt
    23521 tccgccgccc tgaaaaaacg gcaggtgcaa agcgtcctgc tttcgcgcga atctgtgttc
    23581 gcctcgcccg aagccgccgc cctgtccgca ctcatcggct tctggctcga accgcgccgc
    23641 gccggaacgc tgcgttttgt cctgacaagc agcatattcg gttatgacgc gcagcaattg
    23701 cacgacttca accaaaacga aagcgagatt ttgcattggg cggaatctgc ccgaaccgca
    23761 ctcgacaact ggaataaata cggcattttc gccgctatgc agcaattttc ccaaacacac
    23821 ggcatcgaaa cgcgcctctt aagccggaac aacgggcgca gtctgaccaa ttatttccaa
    23881 ctgctcgaac tgcttgccgc cgaagacgcg caaaaccgca accccgccgc gctgcacaaa
    23941 tggctgggcg accaaatcag ccttgccgaa aacaacagcg gcgacaaccg cgccatccgt
    24001 ctggaaagcg acgaagattt ggtcaaaatc gttaccatgc acgcctcgaa aggtttgcag
    24061 tatccgctgg tgtactgccc gtttgcgtgg gacgcgcaag ataccggacc gtccgactgg
    24121 caaatcctcc accaaggcgc aaaccgaacc gaactgttgg caaaggcgca actgtcggaa
    24181 gacgaacaga aacaatacgc cgatgaagaa atggcggaac gcctgcgcct gctttatgtc
    24241 gcgctgacgc gtgccgagga acagctcaac atctacgccg cgtactccac caatactgcc
    24301 gacaaccccc tcgcctacct gattgaaggc tcgccccaag acagccgtga aaccgtccgc
    24361 cgtgcctatg cgtgtgaaaa agacggcatc gcgatgctca aacgcaactg gcggcgcgtg
    24421 gcggacaacg ccccttccgg cacaaatttc gccttcaccg aaaatgcgcc gccccctgcc
    24481 gcctatcgca gcaacgccgg tcaaaccgcc gaatttgccg caaacagcat tcccgaacgc
    24541 ggattccgat ttgtccgcca cactagcttt accgccttaa gccgccatac ccaaacgccc
    24601 gacggcggcg aagaagatgc ctgcccgtcc ttggatgccg ccgaaacctc ggtgccggcg
    24661 atgccgtctg aaacgccgac ggcttcagac ggcatatcga tacacgattt tccgaaaggc
    24721 acgcaggcgg ggctgtgcct gcacgaaatt cttgaagatt tcaaattcgg acaagcagcc
    24781 gccggacagg aaaccctcat tgccgacaag ctgaaaaaat acggttttga agaaatatgg
    24841 ctgcccgccg ttgccgaaat ggcggaagcc tgccgcaaaa cgccgctgac gggggcatac
    24901 gacctgtccg acatctcacc cgagtgccgc tgtcccgaaa tgggctttac cctccacacc
    24961 gaagacttca gcctcaaacg cctgcgcgac tggtttgccc gcgacgacat caggctgccc
    25021 gaagtctgcc gtgccgctgc cgaaacgctc gacttccaca ccgtcaacgg ctttttaaac
    25081 ggctttgtcg atatggtctg ccaagacccc gacggcaata tctgcgtcat cgactacaaa
    25141 tcaaaccacc tcggtacgga cgcatccgcc tacacgcaac aggcaatgga cgaagccgtc
    25201 gcacaccacc actattacct tcaggcactg atttacgccg ttgccgccgc acgctacttc
    25261 aagctgcgcg gacaaccgcc cgccgccgtt tccgtccgct acctgttttt gcgcggattg
    25321 gatggcaaag gcggcggcgt ttggcgttgg gacatagatg ccgccgcttt ggaacagata
    25381 aaataacttc ccgtcctttc ggacaacaac cgcaggaaaa ccctatgaat atcaaacacc
    25441 tgattaccgc cgcactcatt gcctcagccg cctttgccgc gcaggcagcc ccgcaaaaac
    25501 ccgtatccgc cgcccaaacc gcgcaacatc cggccgtttg gatcgatgtc cgttccgaac
    25561 aggaatttag cgaagggcat ttgcacaacg cggtcaacat ccccgtcgac caaatcgtcc
    25621 gccgcataca cgaagccgcg cccgacaaag acacgccggt caacctctac tgccgcagcg
    25681 gacggcgtgc cgaagccgcc cttcaagagc tgaaaaaagc aggttataca aatgttgcca
    25741 atcacggcgg ttatgaagac ctgctcaaaa aagggatgaa atgacgcaat gccgtctgaa
    25801 aggacaggct ttcagacggc atacggcgtt ccggcacgcg gcgcaacatc gggcgcggcg
    25861 cgcgccgccc cgtccgaagc ctgataaaca aaccgataag gaacaatgat gtttcacgac
    25921 acataaagca ccgccctatg tgttgccctg atttggaaag ggttacaccc ctcccgaata
    25981 aagtctgatt ctaccgtttc aaccaaaaag gaaaaaggaa atacgatgaa acacatactc
    26041 cccctgattg ccgcatccgc actctgcatt tcaaccgctt cggcacatcc tgccagcgaa
    26101 ccgtccaccc aaaacgaaac cgctatgacc acgcataccc tcacctcaaa atacagtttt
    26161 gacgaaaccg tcagccgcct tgaaaccgcc ataaaaagca aagggatgga catttttgcc
    26221 gtcatcgacc atcaggaagc cgcccgccga aacggcttaa cgatgcagcc ggcaaaagtc
    26281 atcgtcttcg gcacgcccaa agccggcacg ccgctgatgg tcaaagaccc cgccttcgcc
    26341 ctgcaactgc ccctacgcgt cctcgttacc gaaacggacg gcaaagtacg cgccgcctat
    26401 accgatacgc gcgccctcat cgccggcagc cgcatcggtt tcgacgaagt ggcaaacact
    26461 ttggcaaacg ccgaaaaact gatacaaaaa accgtaggcg aataagcccg tcgccgacat
    26521 atggcggatg gaaatcaaat gcacacgccc ctcattccca cgaagtcggg aatccaatcg
    26581 gttgggttac cgtgattttc aatcattgcc gaaacggacg attccggatt cccacccttg
    26641 cggggatgac gggcgtgtgt gtttttgcca catcacaggc aactttgccg caaatcttat
    26701 ctgtcgccgg tatgccttct tgtttcaatc cgccacaaac caaatgccgt ctgaaacccg
    26761 atttcagacg gcatttccgt ttattccctg ctgtcgcggc aaatatcgat ggcttcctgc
    26821 aaactcgaaa cgccgtagat tttcaggttc ggaaactctt tggcgttgcg cggcatattg
    26881 gctttgggga cgatggcgcg tttgaagccg agtttttccg cttctttgag ccgctcttgc
    26941 ccgcgtgcga cggggcggac ttcgccgctt aagccgattt cgccgaaaac cacggttttt
    27001 tcaggcatag ggcggttgcg gaagctggaa agcatcgcga ggatgaccgc caaatccgcc
    27061 gccggttcgc cgattttcac gccgccgacg gcgttgagga acacatcctg atcgaaacag
    27121 gcgatgccgc cgtggcggtt taacacggca agcagcatcg caagacggtt ttgttccagt
    27181 ccgacggtga ggcgtttggg cgtgaagccg tgcgcgtcat cgaccaatgc ctgaatttcg
    27241 accaaaagcg ggcggctgcc ttcctgtgta accaaaacgc acgagccggg cgtatcgtcg
    27301 cggtagctgg cgaggaagat ggcggacggg ttggacacac ctttcaaacc gttttccgtc
    27361 atcgcgaaca cgcccagttc gtttgccgcg ccgaagcggt ttttgatggc gcgtatcatg
    27421 cggtagttgg aatgttggtc gccctcgaaa tacagcacgg tatcaaccat gtgttccagc
    27481 acgcgcgggc cggcaatcgc gccgtctttg gtcacgtgtc cgaccagtat catggcgatg
    27541 cccatctgtt tcgccatgcg cgtcagttgg gcggcacact cgcgcacctg cgacacggag
    27601 ccgggggcgg acgtgatttg gtcggaatac atggtttgga tagagtcgat gacgacaact
    27661 tcgggctgat gctgtttcaa ggccgcctga atcgcttcca tgcggatttc ggcaagaagg
    27721 tttacgccgt cggtcggcag ttccaaacgc tgcgcgcgca gggcgacttg ttgggcggat
    27781 tcttcgccgg aaacgtatag cactttacgg ctttgcgcca ttttggcgat ggtttgcaac
    27841 agcagcgtgg atttgccgat gccggggtcg ccgccgagca ggatgaccgc accatcgacc
    27901 aaaccgccgc ccaatacgcg gtcgagttcg cccataccgg tcggattgcg cggcacttcg
    27961 gtggcggtaa cggcggagag ggattggacg gtcgaggtat ccgccgccca agattggaaa
    28021 cgggcgtttt tcggctcggg cgcggcaagg ctttcctgaa gcgtgttcca ctcgccgcaa
    28081 tgcgggcatt tgccctgcca tttcggcgaa gtgccgccgc attcggtgca ttggtaaagg
    28141 gttttaagcg tttttgccat cggttttccc tgcatctgaa aacaaaaatg ccgtctgaac
    28201 atagggacgg ttgggattgc gccaatacga caacgccgcc gtcatgtact atccgtacac
    28261 ggcggatatt gccgcattgc cttattttta ccctaaccgg ccatctctcg gcttcagacg
    28321 gcatatcggg cggtttcctg ccggcttacc cgccgtgata ctgccgagac agctcgtgta
    28381 cggtatcgac taagattttg gcgtgttcgg ggtcggcgtg ttggttgatg ccgtgtccga
    28441 ggttgaagac atggccgctg ccgtgtccgt agtcggctag gatacgtgcg acctcggtgc
    28501 ggatggattc cggcgtaccg aagagggcga acgggtcgaa gttgccttgc agggcgactt
    28561 gcttgccgac gcggcggcgt gcttcgccga tgttacacgt ccagtccaag cccaatgcgt
    28621 ctgcgccgat ttgggccata ctttccagcc acagcccgcc gcctttggca aatacgataa
    28681 caggcacgcg gcggccttcg ctttcgcgtt tgagtccggc gacgatctgg cggatgtatt
    28741 tgaggctgaa ttctttaaac gccgcatcgc tcaacacgcc gccccaagtg tcgaaaatct
    28801 gcaccgcctg cgcgcccgcg tcgatttggg cgttgaggta ggcggtaacg gcttgggcgt
    28861 tggtatcgag gattttgtgc agcaaatcgg ggcgcgagta catcatggtt ttgatggtgc
    28921 ggaattcttt gctgccgccg ccttcgacca tataacaggc gagcgtgaac ggactgccgg
    28981 agaagccgat gagcggtacg cggccgtcca atgctttacg gatggaagtt accgcgtcga
    29041 aaacgtattg cagtttttcc atatcgggaa cgtgcagctt ggcgatgtcg gcctcgtgtt
    29101 gcagggcgcg tttgaatttc gggccttcgc cttcggcaaa atacagtccc aagcccattg
    29161 cgtcagggac ggtcaggatg tcggaaaaca aaatcgccgc gtccaaatcg aaacgttcca
    29221 aaggttggat ggtaacttcg gtcgccaatt cggtgttttt gcacaaatcg aggaagctgc
    29281 ccgctttcgc gcgtgtggct ttgtattcgg gcagataacg ccccgcctgg cgcatcatcc
    29341 aaatcggcgt gtattcgaca ggttgtttga gcagggcgcg gaggaaagtg tcgtttttca
    29401 aagagatcat gtggggcttt cggtttgttt gtaaaacggg gaaggaaaac ggttttcaga
    29461 caacctttgc ggcggtttcc gcaatgaaag gtcgtctgaa aagggaggga cgggcacatt
    29521 tgcctaagta atcatcagta gatgattaaa cggcagcagg ttttcatggg gtttcaatgg
    29581 tgcgtttatc ttcgatatgg ataactttgg gttcttcagc atcacaatcg cgatgagagc
    29641 gcccttcgat accgattttg aaatcttcat ccaatccttc ttcttttagg attagcgtaa
    29701 aaatttcggg atgatggcat gaaacggcat atccatcgcc actatctaaa gtgataatgc
    29761 caaaatctgc agatggtgtt cctatttgaa ccttgcccca tttgcttaac atctctttag
    29821 cttgtttcgc caaatctttt tccggttcaa gtaaaatgac gcaagtccca ttttcaaaga
    29881 gcacccaaga tttttcgttt ccattaatgc tctttttcca aacatcaatt aacttttggc
    29941 gataagcagg cgttccaatt tcaaatattt ctttcttttc cttaaaggaa tctgaagacc
    30001 ccatcaaatt ctcccagata aagatatttc ccaaaatcag ctatgctgct ctaacgctgc
    30061 gtgacgttcg tcatcggaga cggcttccaa aaccaagttg cgctgcgcct ccgccttctg
    30121 cggttctccg atttcgtcga aaacctttgc tagaaccaaa cgcgcggaaa tactcggctt
    30181 taatgcaatg ctcgcttcaa ggtagccttt tgccttgccc caaagtttgc ggccgtaggc
    30241 gagccgaccg agatacatca gcagaagcgc attatcgggc tgttctttca gccaagcatc
    30301 ggcaaaatcg atggctttct gctgatcgcg ttcgcccaaa aagcgcacgc tttcgacaaa
    30361 gacttccaaa agttcgggtc ggcggttgtg cggataatgc tgtttgaccc atttgaccgc
    30421 atcggcatac agtcccaaac gttcgtactt ttccgcaacc gatacgctca attccccgtt
    30481 tttgaggctg tcgggaatcc gcttcaggca ggttttcaaa gcggcggcat cggcagcatc
    30541 cgccagctgg cggcggtatg cccaattttg ataccgttcc atttccgatt tgcccaacgc
    30601 gcccgccttg gaaagttttt cggtttttgc cagaacctgc aacgcgtcgc ccctgtcgaa
    30661 agcgtaacga agttgcagac gcacgaggcg cgtaaggttg gcattcatct tcgccgccgc
    30721 atgaagattg gcttccgccg cttcgtaatc gcgccggttc aacgccgatt ccgccaacaa
    30781 aagataacgg gaaagctgct gtttttccgg cagtttggcg atttccgcaa gataacggtc
    30841 gcgcagctcg atgttttcca tctgtccggc ggcgtgcgca cccagcatca atgccaaagt
    30901 ccggttgtcg cccgcctctt tgttgcccaa cacgcgcgag gcttcaagtt ccgccttttc
    30961 aaaacgccct tcaaaatacg ccaaacccgc cttgttcaag gcaagcgcgg ccttgcggcc
    31021 tttacgcgcc gaaccgaaac gctgcatctt ttcggggata ttgaatacgc cgataatgaa
    31081 tttaaacagg aaataccaca ccacgacggc aatcagcgaa cctaacacaa aagcgtgcag
    31141 gttgattctg agcatggtct gtccgagtac gatatacacg tcgccggtgt aaatgcccga
    31201 agccagcgcc agtccgacgg cggcggcaaa caggacgaca atccagacta ccgttttcat
    31261 gcgcgttctc ctttagcgga cggcgaacgc cctgcctttt ccggcacgga ctgttttttt
    31321 gcaggctgtt ccggtttgcg ttccgaaggc agggacggtg cttctatggt cttcacatcc
    31381 gatgcggcag ccggttcgga agctgtgttt tcgtttgccg gttcggaagc cgcctgttct
    31441 gcttcttgat tttccgccgc cgtcatgcgc gtaccgtcgc gataggcgcg gacggcattt
    31501 aggctgtttt tcaaaccgtc atccgccgtc atgcgcacat ccaacgcctt caattccgcc
    31561 agttctttca gccacgactg cgtggcggga gacttggcat cgaaatactg tctgacggcg
    31621 gcttcggcat tgttcaaatc gccctgatag acttcgctgt tgcgctgcat taatgcagtc
    31681 cgcgcatcca aaaggcggag gcgcaggttt tcacgcacaa aatatgcctg ttcgggagaa
    31741 atcagcatgg catcgttgtt ttccaaacgg cggatttcga ccagcccctt caatgtgccg
    31801 agggattttt cccatacgtt ctgccaccat gaagcagaag cggcttcgtt cttcacctgt
    31861 acgcccggtt tcaatacgcc gtcgagcatc agcggcagtc cggatacggc ggtttccagc
    31921 ctgtcgaggc gcaatgccgt gccggaaata tcgacatagg gacggttttt cagttccgcc
    31981 aagtcgctgc tgaccgcctg cttgatcggc agaagctctg cctgattgaa acgggacagg
    32041 cggctgtcga tatgctccaa tacgccgact gccgtttgga tattgccagt caacaccagc
    32101 tgttgcgccg ccagattgag tatggtctcg gtttcgtcca ccagccaatc ggcgcgtcct
    32161 ttggtcaact cgcgatagga tttttgcatt tccaagattt gttcgccgtt tgctttgacg
    32221 tttccgtcca aacggtcgag ctctgattgt atggcggctt gccggttgag gttgtctttc
    32281 aacagggcgg cgttttccga ctcgcccaag gcggctttgt cgattttttg gttgaatgcc
    32341 agctcttggt ttttcaagac attctgtcct tggacaaaca aaaaaccact tgtacccaaa
    32401 cccaatgccg ccaataccag ggcgcagact gccaaagcgt tgctgccgga ctgtttgacg
    32461 ataaaaggag cggcaggaat gtgtacttct gtttcgtttt ctttgcgtgg ggaagaggtt
    32521 tcagacggca tttcttttga tgcctgtatc tcgcgtacgg gttcggatga tttgttttca
    32581 ggttcgccca ccgttttgct ccttattaat ttgaggttcc gggaaagacc atgccgtctg
    32641 aaacggaaat ggaagaatgg gaaagcgcgg cttccagcgt agggacggtt tcgaccgaac
    32701 acacgccttc gcgcttcaat gcctccgcaa tgcgcggatg atgggtaaag tatagcaagg
    32761 atttgaagaa tcgggaaaat tgcggcggaa gctgcccgaa cagcaagcgc accagctcgg
    32821 tcgacgtaat ataggcggcg gcaatatttt cggtttggaa attttgaaag ttcaaaggtt
    32881 tatgccgtct gaaatagact tctgccatct ccgtccgcaa acctttctcc tgcaaggcat
    32941 tcatcagaaa atctcgcccg ccgtgtccgc gcacaaacaa tacgcgcgca ccttcgggca
    33001 gactgttcca aaccggcagg cgcaaaaccg cctcgctgtc gttgccgtca tcaggcgcga
    33061 tgaccgttct gcccaaacag cgttccaatg cgcggcggct gcccttccct acggcaatgt
    33121 gcatctttat gccgtctgaa aggttaaggt acgggacggc ggtttcaacg gcggtcggac
    33181 tgacccaaaa cacggcatcc gcacgggcat attgttcgga caaaagttcc agtccggcag
    33241 catctgcttc gatttcgacc ggactcaata cttccgcgcg ccaaccggca ttcaggcaag
    33301 tttcgacatc ttctgcggcc ctgcccgacg gacggacaat cagcatgacg ggtttcgctg
    33361 tttccatcgc cttatccgcc ccgattacta ggaaaaatcg tcatcgttga aattcgcctg
    33421 cgcggttttc ggcgtacagc cgacccgtgc gacttccttg cccctacgct ccacaacgac
    33481 accgctgctt tccgtatgag tcacggaatc gaagcctgtc catatggtgt acataaattc
    33541 gccgttttgg aatttcatcg ttgcccaacg accgctgccc ataccttgcc acctgtcgga
    33601 acgccccaac aggtcagctt tgctgttgcg tatggcaatt tctttttttg ccgcactgcc
    33661 gaacgaatat tcgtaaagat tgcggttgat tttttgaact tctatatatt tgttgttgtc
    33721 cgttttacag gaaaacaccg tatccgccca cacggttcgg gtcgcagccg acagaagcaa
    33781 tacagcagaa aggaacattt ttttcatcgt atttccttaa tggttgaaac tccgccattt
    33841 ggacatccgt ccttcgtgac agcagcatca gatgttattt gagaggggtg taaccccttc
    33901 caaattaggg caacacatag ggcaacgctt tatgtgtcgt cctgtgtgtt gaaacatcat
    33961 tgcccgtatc ctgaaataga aaagaaaatg cccgttttac ggtcttcttt ccgacaaaat
    34021 gccgtctgaa acggatttca gacggcatgg tcgaaaatta ttgcgcggca agcggcttga
    34081 gcagcaaggt ttcgccgctt tgcacaactt tttcaagcag gtcgatattg ggatgcttgc
    34141 cgggatgggc gcgcgcgtcg gcaacgtgtt cggcaaacca tatcaaaccc tgttctgccg
    34201 cttctttgtc caattttccg tcaaaacgtt ctgccaaagc gttgtacaac ttgagcgaac
    34261 cgagcttacc ctgaacggca ggaatgtggt gaaccgtttt gccatctgca tcctgaacgt
    34321 ccaaaccgcc cagatggtcg attgaagaaa atgtggcgag atagtcttga aaattcatat
    34381 caaatccgaa aatcagtcag ttagcgcgtg ccgaaaatct tgtcgcccgc atcgcccaag
    34441 ccggggatga tgtagccgtt ctcgttcaag tggctgtcga gcgcggcggt gtaaatcgta
    34501 acgtcgggat gcgcgtcgtt gaccgccttc acaccctcgg gcgcggcaac cagcaccagt
    34561 gccttgatgt ttttgcagcc cttggctttc aacaggtcga tggtggcaac catcgaaccg
    34621 cccgtcgcca gcatcgggtc gataatcagg gcaggacgtt cgtccatgct gtccacgaat
    34681 ttctcgaagt aggaaacggg tttcagcgtt tcctcgtcgc gctgcagtcc gactacgctg
    34741 attttggcag tcggaatcag gtcgagtacg ccgtcaagca tacccaaacc tgcacgcagg
    34801 atgggaacga cggtcaatgt tttgcccttg atgcgatcgc cttcaatctg accgcaccat
    34861 ccgtcgataa ggtatttttc gatttcaaaa tcacggcttg cctcgtatgc catcaggcgc
    34921 gccagctcga tggcaagcgt ccggaatttg taggtgctgc aatccgcctc cctcatcagg
    34981 gttaatttgt ggcggacgag cggatggttg ataacattaa cgttcatcgc ggtgttccag
    35041 tgtgattttg agacgcgcca ttatactttt ttttgatttg ccggaaaaga aaatgccgtc
    35101 tgaaacacgt tcagacggca ttgccgaatc catcaggctt tcagcagctc ttgcagctcg
    35161 cctgcctcat acatttccat caggatgtcc gaaccgccga caaactcgcc gttcacataa
    35221 agttggggga tggtcggcca gtcgctgtat tccttaatgc cttggcgtac ttcgggattt
    35281 tccaatacgt tgacggtaac gtaatcggtg cagcctgccg cgttcaggat ttgcacggcg
    35341 cgggaagaga aaccgcattg cggaaactgc ttcgtacctt tcataaacaa tacgacgcgg
    35401 tgtgtcgtta ctacttcttt aatttggtcg tggatggaag ccatcgctta ttccttttga
    35461 taaagttgac aaaatttgtc aggtaggacg cattatacgc aaaagcggaa acttcccaca
    35521 atatttgccg tatatcgcgc agtatcaatg ttgtgaacgg aaatattccc actcctcgac
    35581 aatcctgctg tccggcaaat ggcacagggc atatccgccg ccgctttcgt ctttttttac
    35641 ttcccgtctg ccgcctttgg caatgcagaa tttggaagcg gaatttgcca tgcctgtccg
    35701 aaacgtgttg gcaggcagcc ggcagcagcg cggacatgca tacggatgaa ttgccttcat
    35761 ggttttcctt atcgtcggta cagggtcaga cggtccgcat atcgggtact ttttgaaaca
    35821 gccggtaaaa gttttccgtc gtgtatgccg caacctgttc caaggtttgg ttccgcaatt
    35881 tggcgatatg ttcggcagta taacgcacga aagccggttc gttctgcctg ccgcgcttgg
    35941 gtacgggggc gaggaacggc gcatcggttt ccactaaaat gcggtcgtcc ggaacgtatt
    36001 tcgccgcctc ctgaaccaag ggtgcgtttt taaaggtaac gattcccgag aaagaaatat
    36061 aaagccccaa atccattgct gcacgggcaa aaccgatgtc ttcggaaaaa cagtggataa
    36121 cgcccgaatt aacccggcat tctttcagga tagacaaggt gtccgccgcc gcatcacgcg
    36181 tatggacgat aacgggcagt ccggtttgat tggctgcttc gatgtggtct gcaaagcgtt
    36241 tgtgctgcca ggacaaatcg cctttgcacc agtaataatc caagcccgtc tcgccaatgc
    36301 cgaccacttt cggatgggcg gctgcggcaa ccatttccgc aatagaaaat tcttcggctt
    36361 ccttgctgtc gggatgtacg cctatggtgc aatagatgtg ttcgtgcgct tcggcgatgg
    36421 caaagacttc ggagaagctt tccctgctga cgctgatggc gagcgcctgc cccacgccgt
    36481 ttgcttccat gttggacaaa acttcgggca ggcgttcttt caaaccttca aaattgaggt
    36541 ggcagtgcga atcgatgata tgcatggcgt tacatggtaa acgtaggtct gctgccgccg
    36601 attgtgttgc ccagttcgac ttcgatctga tctttgactt tcaggcagtt ttcgtgtttt
    36661 gtgaatgcca gccctacccc tttgggtttg gaggaagtac gcgcaggatt gatccaggcg
    36721 actttggtcg gcaggaacag tttggggaag ttgaggattt ctacggcaag cagaatatcg
    36781 tccccgatgg aaaatacgtc gttggtctgc acaaacagac cgccgtgttc caaaaacggc
    36841 atgtaggagc tgtacagcag attcatgtct ttcagctgca acgacatcat ttttgccgga
    36901 atattttgtc cgtctgacat aattcacccg ttctctttct tcaattccaa atagttgatg
    36961 agcagatgct cgatctgcat tttgacattt aaagtatgaa acccgtaggg ggcaagctgt
    37021 ttgagcatat cctccgccgc aaatacattg cgcggacgga aaccggatac cgtctgaagc
    37081 agcctgtctt cataagcggg ataatagacg ggtttcatgt gttgcaggca caatcccaaa
    37141 tcgaccagcc acttctgcat ccacccgaca aatacggcga gcggaagttt ttccttatcg
    37201 aaaagcgcgg cgtaatccaa aatcttcaac aaccttggtt ctgccaaaat atccaacagt
    37261 ttaatccgca acgcacggac accgtccgcc tcatcaaaca gcggcgctcc ggaatggaaa
    37321 gccagacgtt cctcaggttc cgccacaccc ctttcacgca gatatgccaa tgcctcttca
    37381 tgggaaggcg cgggcaaaac catcttccgg cagcggcttt taatggtcgg taaaaccttg
    37441 tccgccgcgt ggctgaccag caaaaagacc acttgtggcg gcggctcttc caacactttc
    37501 aacaaactgt tggcggcttg gacattcata ctttccgcag gatgaatcag aatcacgcgc
    37561 aaaccgcccc gtaccgaagt caggtacaca ttatcgatga tttccctgac ggcatcgatt
    37621 ttgatctgca acagtttgcg tccgttttcg ggttcgtccg acaagggggt gatttcgtaa
    37681 aaatcgggat ggcttccctg tccaaacaga tggcaggaca tacattcgcc acagggtttg
    37741 cagcccggtg caggggtttc gcacaacagt gccttcgccg caaagcgggc aaatgtagtt
    37801 ttccccgtat cttttttgcc ggcaaacagc catgcattgg gacgacgctc ccaatgttcc
    37861 gcaatctgcc gccattgctc attatgccac ggataaatca tttcaaacca tccgtccgga
    37921 aatccatacc gccatcagag cagttccaaa tcggggctgg aactttgcgc gcggcggctg
    37981 cgcaactgta ccgccaaacg caaatcgtgt gcggaatcgg catttttcaa tgcttcttgc
    38041 agggaaatat cgcctttttc atacaattgg taaaggtgtt gatcgaaggt ctgcataccc
    38101 agggtagtgg attttttcat cacttctttg atttcatgga tgttgccgtt gtgaatcaac
    38161 tccgaaatca ggggcgaatt gagcagcacc tcgactgccg ccaccctgcc cttgccgccg
    38221 tctcgcggaa cgaggcgttg cgaaataaac gcctgaaggt tgagcgacaa atccgtcagc
    38281 aattgttcgc gccgctcctc ggggaaaaag ttgatgatgc ggtcgagtgc ctgattggtg
    38341 ctgttggcgt gcagcgtcgc catacacaaa tgccccgttt cggcaaaggc aatggcgtag
    38401 tccattgttt cgcggtcgcg gatttcgccg ataaggatga catccggcgc ctgacgcagc
    38461 gtgtttttca acgccgccat ccagttttcc gtatccacgc cgacctcgcg ctgggtgatg
    38521 atgcagtttt tgtgttcgtg gacaaactcg atcgggtctt cgatggtgat gatgtgtccg
    38581 aacgaatttt cattgcggta atcgataagc gaggcaagcg aagtcgattt gcccgagccg
    38641 gtgccgccga caaaaataac cagcccgcgt tttttcagcg cgacatcctt caagaccggc
    38701 ggcaggttca ggctttcaaa cttgggaatc ttgctggtaa tcgcacggaa taccaacgcc
    38761 gtcgcgccgc gctgtatcat cgcattgacg cggaagcggc tggtgtccgg caggctgatg
    38821 gcgaagttgc actcgttggt cgatgaaaat tcttccgcct gcttcgcact cataatcgaa
    38881 aaggcgattt ccatacattt ttccgccgtc agcggttcgt ccgtgatgcg ggtgattttg
    38941 ccgtccagct tcattgcggg cgggaaatga gtcgtcacga acaggtcgga acctttgttt
    39001 tggttcatat ggcgcagcca tgcgaacagt tcttcctttg cggtcatatt ttcatcggta
    39061 aacatcctcg tcccctaatc ttcaaacaat ctgtaccttc ccattctacc aaaaacccgc
    39121 cgtgccgtct gaaaccgttt cagacggcac ggacaaacca cctccactca atcctgccgt
    39181 gttagcgtgt ataatcaggc attctatccg aaattcacaa caggtcagca atatgtcttt
    39241 gaaaacagta gccgttatcg gcgcaatgga acaagaaatc gagcttttgc gcgagatgat
    39301 ggaaaatgtc aaagccgtct ctttcggcaa gtttgccgcc tatgaaggcg aattagcggg
    39361 aaaacgcatg gtgcttgcat tgagcggcat cggcaaggtc aacgcggcgg ttgcaacggc
    39421 ttggattatc cgtgaattcg caccggactg cgttatcaac accggcagcg cgggcggctt
    39481 gggcaagggt ttgaaagtcg gcgatgtcgt ggtcggcaca gaaatcgcgc accacgatgt
    39541 cgatgtaacc gcattcggct atgtttgggg acaagtgccg caactgcccg ccgtatttgt
    39601 ttcagacggc ctcttggtcg gcaaagcaaa acaggcggcg gaagtgtttg aaggcgcggc
    39661 ggtagaacaa ggcctgattg tcagcggcga ccgcttcgtc cacagcagcg aaggcgtggc
    39721 ggaaatccgc aagcacttcc ccaaagtcaa agcagtggaa atggaagcgg cggcgattgc
    39781 ccaaacctgt catcagttgg aaacgccttt cgtcatcatc cgcgcggttt ccgattcggc
    39841 ggacgaaaag gcagacatca gctttgacga atttttgaaa acggcggcgg caagttctgc
    39901 aaaaatggtg gcagaaatcg tcaaatcttt ataaaacctt tatcaaatca tgccaaaaca
    39961 gcggttttcc gttagaatat cgggctattt ccacgttgcg cccgatattc ggacgcaccg
    40021 ctgtttgttc agacggcatt tctaaaacct accgttcagc attaaaaaca actcaatgaa
    40081 aaatatccga aatttctcca tcattgccca catcgaccac ggcaaatcga cgcttgccga
    40141 ccgcttcatc caatattgcg gcggtttgga tttgcgcgaa atgagtacgc aggtactcga
    40201 ttccatggac atcgaaaaag agcgcggcat taccattaaa gcgcaaaccg ccgcgctcaa
    40261 ctacaaagca cgcgacgggc aggtgtatca actcaacctg attgacacgc cgggacacgt
    40321 cgacttctct tacgaagttt cccgttcgct gtcggcttgc gaaggcgcgc ttttggtcgt
    40381 tgacgcgtcg cagggcgtgg aagcgcaaac cgtagcgaac tgttacaccg cgattgattt
    40441 gggcgtggag gttgtgcctg ttttgaacaa aatcgacctg ccagccgccg accccgaacg
    40501 cgtggagcag gaaattgaag acatcatcgg catcgatgcg gtcggcgcgg tgcagtgttc
    40561 cgccaaaagc ggcatcggcg tggaagacgt tttggaagaa atcgttgcca aaattcctgc
    40621 gccgaccggc gatgaaaacg cgccgctgca agcggttatt gttgattcat ggtttgacaa
    40681 ctacgtcggc gtagttatgt tgattcgtgt gaaaaacggc accatcaagc tgaaagacaa
    40741 agtgcgcttt atgagcacca aggcggaaac tcaggttgag cagctgggcg tttttacgcc
    40801 gaaatcagtt caaaaacaag aactcaaagc cggagaagtg ggctttttga ttaccggcgt
    40861 gaaagaattg ggacaggcga aagtcggcga tacggttact ttggttgcca accctgcctc
    40921 tgagccgctg cccggcttcc aagaggtgca aagccaagta ttcgcgggtc tctaccccgt
    40981 agaaagccac gactatgaag ccttgcgtga cgctttggaa aaattacagc ttaacgacgc
    41041 ttcgctaaaa ttcgagcctg aagtttccca agcattgggc ttcggcttcc gttgcggctt
    41101 cttgggtctg ttgcacttgg aaatcgtgca ggaacgcttg gagcgcgagt tcgacatgga
    41161 tttgattacc accgcgccga cggtggttta tgaagtcgtg ttgaaaagcg gcgaaaaaat
    41221 cgaagtcgaa aatccgtcca aactgcccga catcggcagc atcgaaacca ttctcgagcc
    41281 gattattacc gcgaccatcc tcgtgccgca ggaatacgtc ggcaacgtca tgactttgtg
    41341 taaccaaaag cgcggcgttc aggtcaatat gcaatatatg ggccgccaag tcatgctgac
    41401 ttacgatttg cccatgaacg aagtcgtcat ggacttcttc gacaaactca aatccacttc
    41461 gcgcggttat gcttcgttgg actaccattt caaagagttc caaccgtctg atttgattaa
    41521 gctggatatt atggtcaacg gcgaaaaagt cgatgcctta agcctgattg tgcaccgtca
    41581 aagcgcggtt caccgaggcc gcgagctggc atcgaaaatg cgcgagctga ttccgcgcca
    41641 aatgttcgac attgccgtcc aagccgccat cggcagccag attatcgccc gcgaaaacgt
    41701 caaagcactg cgtaaaaacg tcttagcgaa atgttacggc ggcgatatta cgcgtaagaa
    41761 aaaactgctt gaaaaacaaa aggcaggtaa acgccgtatg aaacaagtgg gcaatgtgga
    41821 aattccgcaa agcgcgttct tggcgatttt gcaggtgagc gacaaataac tctggcacac
    41881 agggcggcgc gtcgggaaca cccctcaaac cgccctaatt tcaggggcac gcgcccgcaa
    41941 ataaattttg caatttgccg ccttaaaacg gttgcacgga caggcgggtt tttaagcatt
    42001 aaggaacaac aatgaacaca atgttaatgt cgggtgcggc tgccgcgctg cttgccggca
    42061 tcatccttta tttcaaaagc gacaagaaac gacaggaaaa cggagaatgg agttccggtc
    42121 tcgaatacgc ctatatcctg acggcggtcg gcgtgtttgc cgctttgtcc ctgtttatga
    42181 gctttaccgc cgttttcctg attttcgttg tattgtgcgg tacggcttgg ggggtataca
    42241 aataccgcct gaggactcat cccgaaatct cggaaagcag ccacttcggc gattatttcg
    42301 gcagtttctt ccctaccgtt ttggtattgt tcctcatccg gtcgtttatc gccgaaccgt
    42361 tccaaatccc gtccagctcg atgcgcccgg gcctgatcaa gggcgatttc attttggtcg
    42421 gcaaattttc ctacggcctg cgcgtacccg ttttaaacaa tgtattcatc cccacgggca
    42481 aaatcgaacg gggcgatgtg gtcgttttta attatccttt gcaaccggag atgacctaca
    42541 tcaagcgtat tgtcggcatt ccgggcgatg tggtcgaata tcggaataaa gttttgacgg
    42601 taaatggcaa acccacttcc gatattcctg acggcacata ccgttatccc gacgacaccg
    42661 acccatccga aatccacaac acggatatgt tccgcagcgg tttggacggc aaatccttca
    42721 atattctgaa aaaagaagga cagccggccg tttccctgcc cgtattgggc aaatatacct
    42781 ccgatattat gtctgaaaac ggatattcca tagagcaaag cggtttggaa cactgccaat
    42841 atgccgacga cggcagcggt ttcgtgtgca aagttcccga aggacgctat ttcgctatgg
    42901 gcgacaaccg cgacaacagt gccgattcgc gctactgggg atttgtggat gacaagctgg
    42961 ttgtcggcaa ggcaatgttc attttgatga acttcggcga tttcggcagg gccggtacgg
    43021 caatccgtta ggccggaaaa ttacgccgga acatcaaaaa tgccatccga accctgtttc
    43081 agacggcatt tttatcatca aacgccttgt ttccctgccg gcagcctcgg ggataatata
    43141 gtggattaaa tttaatccac tatatttccg tttgtgtgga aacccatccc attgaataaa
    43201 gggcatttcg tccgactgac ggtgtgggcg gcatatggaa atacggacat tattttagtt
    43261 gcattgccat tccagcggag gcggcttttt tgattctgcc cgccccgatg ccgtctgaaa
    43321 gggcttcaga cggcattttc cgctccgcgc cgttgccggc gtgtttggtc ttgccggttt
    43381 tgactgcctg ccagttcaat cgtacgcagg tttgccacgg cagaaaatct gatgccggac
    43441 ggagtcaagg ctcggactgc tgtttcagcg aaccgagata aattgccgaa agcgtcagcg
    43501 ccacccctgc ccactgcacg ccttcaatcg gtttgcccaa tccgaaataa tcgatgaaca
    43561 gggcggcaac cggttcggac aaaagcagca gccccgtcag cgacagcgaa agcagcggaa
    43621 tcgcataggc aaccatcgcc cacgcgaagc actgcatcac cacgccgtac acaagcacca
    43681 aacccgcatc tttccaagtc gtcggataaa gcgcgccgcc gtccatcagc aatgccggaa
    43741 caaccaacga taccgcgccg cctaaactca aaatcatcat tgaagggaaa agcgccaccg
    43801 tctcgatttc atgggttttg cggacgaaca ccatcgacag ggcgagcatc agtcccgata
    43861 ccaaaccgct gacgaatccc caaaccgcat taccgttgta gccgaattcc gcaccggcaa
    43921 tcatcgccac gccggcaact gccgatatta agcctgcctt tttcagcccg ctcaaacgct
    43981 cgccgaaaaa gaaaacaccg attgccgaca agaaaaagat ttgcaggctg ttgagcaggg
    44041 tggaaatacc cggcccgacc gcgtgtatgc tttcgtgcca caacgccaaa tcgaaagcga
    44101 gaaacacgcc cgccgtcagg gcatatcgga cggttttcct gttttttggg aatttttgcc
    44161 tgaaaaaccg tgctaaaaac caaaatacga acaccgaaat cagcaaccgc caaaatgcga
    44221 ttgcatacga accgacgggg acggatctga caatcagact gcccagaccg aacaccacgc
    44281 aacccagcac cagcaatggt gcggcatatt ttttgccgtt accgaccaca atatcccctc
    44341 ccttccccat acggctgccg cgccgacggc aagaatgccg ccattccgaa aatatgccgt
    44401 ctgaagccca aagccccggc tgcacccgga caaacggctg attttacggg caggcaggct
    44461 attttccgtt ggggcaaaaa accgtcctgc cagataaaac ccg
//
LOCUS       DAWFHY010000004        42270 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.004, whole genome shotgun sequence.
ACCESSION   DAWFHY010000004 DAWFHY010000000
VERSION     DAWFHY010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42270
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.004"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(566..841)
                     /locus_tag="WGQ68_000161"
     CDS             complement(566..841)
                     /locus_tag="WGQ68_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0714048.1"
                     /translation="MNASNWSVYLILCENSAFYCGISPNPQQRLAAHTTGKGAKYTRV
                     FKPVAMRIVAGGMDKGTALRQEIAVKKLTAAQKRQLWEQAEKMPSET"
     gene            complement(838..2121)
                     /locus_tag="WGQ68_000162"
     CDS             complement(838..2121)
                     /locus_tag="WGQ68_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0714049.1"
                     /translation="MTASKSGFIGQIFSRNMLVCIFTGFTSGLPLYFLINLIPAWLRS
                     EQVDLKSIGLMALIGLPFTWKFLWSPLMDAVRLPVLGRRRGWMLLTQAGLLAALAAYA
                     FLNPRNHLPLIAGLSVLVAFFSASQDIVLDAFRREILSDEELGLGNSVHVNAYRIAAL
                     IPGSLSLVLADRMPWSEVFVITSLFMLPGLLMTLFLAREPVLPPAVPKTLKQTVVEPF
                     KEFFTRKGIASAVCVLLFIFLYKLGDSMATALATPFYLDMGFSKTDIGLIAKNAGLWP
                     AVAAGILGGVWMLKIGVNKALWLFGAVQAVTVLGFVWLAGFGPFDTVGTGERLMLAAV
                     IGAEAVGVGLGTAAFVSYMARETNPAFTATQLALFTSLSAVPRTVINSFAGYLIEWLG
                     YVPFFQLCFALALPGMLLLLKVAPWNGEKTQDAGR"
     gene            complement(2269..3687)
                     /gene="glnA"
                     /locus_tag="WGQ68_000163"
     CDS             complement(2269..3687)
                     /gene="glnA"
                     /locus_tag="WGQ68_000163"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEZ0714050.1"
                     /translation="MSIKNAVKLIEESEARFVDLRFTDTKGKQHHFTVPARIVLEDPE
                     EWFENGQAFDGSSIGGWKGIQASDMQLRPDASTAFVDPFYDDTTVVFTCDVIDPADGQ
                     GYDRDPRSIARRAEAYLKSSGIGDTAYFGPEPEFFVFDGVEFETDMHKTRYEITSESG
                     AWASGLHMDGQNTGHRPAVKGGYAPVAPIDCGQDLRSAMVNILEELGIEVEVHHSEVG
                     TGSQMEIGTRFATLVKRADQTQDMKYVIQNVAHNFGKTATFMPKPIMGDNGSGMHVHQ
                     SIWKDGQNLFAGDGYAGLSDTALYYIGGIIKHAKALNAITNPSTNSYKRLVPHFEAPT
                     KLAYSAKNRSASIRIPSVNSSKARRIEARFPDPTANPYLAFAALLMAGLDGIQNKIHP
                     GDPADKNLYDLPPEEDALVPTVCASLEEALAALKADHEFLLRGGVFSKDWIDSYIAFK
                     EEDVRRIRMAPHPLEFEMYYSL"
     gene            complement(3961..4338)
                     /locus_tag="WGQ68_000164"
     CDS             complement(3961..4338)
                     /locus_tag="WGQ68_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714051.1"
                     /translation="MKKMLCLAVFALGLATPAVAADWVLVDSNTATFGDADSRTDNSA
                     WFETRFAKPQKYGNGKFFNTAKTLEEMDCSGKRFRILTATWYSKSGNSIASYTPSYAE
                     WNYVIPGTLGEAQYKFVCNGYPR"
     gene            4508..5317
                     /gene="aroE"
                     /locus_tag="WGQ68_000165"
     CDS             4508..5317
                     /gene="aroE"
                     /locus_tag="WGQ68_000165"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEZ0714052.1"
                     /translation="MTALPRYSVFGNPVAHSKSPQIHQQFALQEGVDIEYERICADIG
                     GFAQAVSTFFGTGGCGANVTVPFKQEAFHLADEHSDRALAAGAVNTLILLKNGKLRGD
                     NTDGIGLANDITQVKNIAIEGKTILLLGAGGAVRGVIPVLKEHRPARIVIANRTRAKA
                     EELAQLFGIEAVPMADVNGGFDIIINGTSGGLNGQIPDIPPDIFQNCALAYDMVYGCA
                     AKPFLDFARQSGAKKTADGLGMLVGQAAASYALWRGFTPDIRPVIEYMKAM"
     gene            5319..6020
                     /gene="mtgA"
                     /locus_tag="WGQ68_000166"
     CDS             5319..6020
                     /gene="mtgA"
                     /locus_tag="WGQ68_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HEZ0714053.1"
                     /translation="MFRIVKWLIALPVGIFIFFNAYVYGNIITYRAVAPHRTAFMSMR
                     MKQFEQEGRDVALDYRWVPYNRISVNLKKALIASEDARFAGHGGFDWGGIQNAIRRNR
                     NSGKVKAGGSTISQQLAKNLFLNESRSYIRKGEEAAITAMMEAVTDKDRIFELYLNSI
                     EWHYGVFGAEAASRYFYQIPAAKLSKQQAAKLTARVPAPLYYADHPKSKRLRNKTNIV
                     LRRMGSAELPESDTD"
     gene            complement(6222..6297)
                     /locus_tag="WGQ68_000167"
     tRNA            complement(6222..6297)
                     /locus_tag="WGQ68_000167"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6262..6264),aa:Phe,seq:gaa)
     gene            complement(6488..7222)
                     /gene="lptB"
                     /locus_tag="WGQ68_000168"
     CDS             complement(6488..7222)
                     /gene="lptB"
                     /locus_tag="WGQ68_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714054.1"
                     /translation="MSANVSRLVVQNLQKSFKKRQVVKSFSLEIESGEVVGLLGPNGA
                     GKTTSFYMIVGLIAADAGSVMLDGQELRHLPIHERARLGVGYLPQEASIFRKMTVEQN
                     IRAILEISTKDKSRIDGEVEKLLADLNIERLRNNPAPSLSGGERRRVEIARVLAMKPH
                     FILLDEPFAGVDPIAVIDIQKIIGFLKSRGIGVLITDHNVRETLSICDRAYIISDGTV
                     LASGKPDDLVGNEQVRAVYLGENFKY"
     gene            complement(7296..7826)
                     /gene="lptA"
                     /locus_tag="WGQ68_000169"
     CDS             complement(7296..7826)
                     /gene="lptA"
                     /locus_tag="WGQ68_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HEZ0714055.1"
                     /translation="MIQKICKLFVLIAFFSASPAFALQSDSRQPIQIEADQGSLNQAN
                     QSTTFSGNVVIRQGTLNISAARVNVTRNDKGEQFMKADGSPVRFSQTLDGGKGTVRGQ
                     ANNVAYSSAGSTVVLTGNAKVQRGGDVAEGAVITYNTKTEVYTISGSTKSGAKSASKS
                     GRVSVIIQPSSTQKSE"
     gene            complement(7807..8388)
                     /gene="lptC"
                     /locus_tag="WGQ68_000170"
     CDS             complement(7807..8388)
                     /gene="lptC"
                     /locus_tag="WGQ68_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEZ0714056.1"
                     /translation="MKIRWRYGIAFPLILAVALGGLSAWLGRISEVEIEEVRLNPDEP
                     QYTMDGLDGRRFDEQGYLKEHLSAKGAKQFPESSDIHFDSPHLVFFQEGRLLYEVGSD
                     EAVYHTENKQVLFKNNVVLTKTADGKRQAGKVEAEKLHVDTESQYAQTDTPVSFQYGA
                     SHGQAGGMTYDHKTGMLNFSSKVKATIYDTKDM"
     gene            complement(8385..8921)
                     /locus_tag="WGQ68_000171"
     CDS             complement(8385..8921)
                     /locus_tag="WGQ68_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0714057.1"
                     /translation="MQAISPELQARAAKIKLLILDVDGVLTDGRIFIRDNGEEIKSFH
                     TLDGHGLKMLQASGVQTAIITGRDAPSVGIRVKQLGINYYFKGISDKRAAYEELRAQV
                     GVEEAECAFVGDDVVDLPVMVRCGLPVAVPDAHWFTLQHAAYIAKRPGGAGAVREVCD
                     LIMQAQGTLGAALNEYIK"
     gene            complement(8986..9960)
                     /locus_tag="WGQ68_000172"
     CDS             complement(8986..9960)
                     /locus_tag="WGQ68_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEZ0714058.1"
                     /translation="MAENGKYLDWAREVLHTEAEGLREIAADLDENFALAADALLHCK
                     GRVVITGMGKSGHIGRKMAATMASTGTPAFFVHPAEAAHGDLGMIVDNDVVVAISNSG
                     ESDEIAAIIPALKRKNITLVCITARPDSTMARHADIHITASVSQEACPLGLAPTTSTT
                     AVMALGDALAVVLLRARAFTPDDFALSHPAGSLGKRLLLRVADIMHKGGGLPAVRLGT
                     PLKEAIVSMSEKGLGMLAVTDGQGRLKGVFTDGDLRRLFQECDNFTGLSIDEVMHTHP
                     KTIAAERLATEALKVMQANHVNGLLVTDADGVLIGALNMHDLLAARIV"
     gene            10056..11111
                     /gene="tal"
                     /locus_tag="WGQ68_000173"
     CDS             10056..11111
                     /gene="tal"
                     /locus_tag="WGQ68_000173"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HEZ0714059.1"
                     /translation="MTILSDVKALGQQIWLDNLSRSLVQSGELAQMLKQGVCGVTSNP
                     AIFQKAFAGDALYADEVAALKQQDLSPKQRYETMAVADVRAACGICLAEHESTGGKTG
                     FVSLEVSPELSKDAQGTVEEARRLYAAIGCKNAMIKVPATDAGIDALETLVSDGISVN
                     LTLLFSRVQTLKAYAAYARGIAKRLAAGQSVAHIHVVASFFISRVDGALDTTLPDHLK
                     GKIAIALAKAAYQDWEQYFTTPEFAALEAQGANRVQLLWASTGVKNPAYPDTLYVDNL
                     IGAHTVNTVPDATLKAFIDHGTAKATLTEGVEEAQAQLAETAALGIDVETLANRLQED
                     GLKQFEEAFEKLLAPLV"
     gene            11265..11555
                     /locus_tag="WGQ68_000174"
     CDS             11265..11555
                     /locus_tag="WGQ68_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714060.1"
                     /translation="MAKALEIISPNEIYSDLIFKDPVPPHTIYRELMKLVSRNASNER
                     IADRAFDFFSSGLMDGSATEEQYNALYDLTLLEEPGMELNKDEIMALINSLK"
     gene            11572..12459
                     /gene="gluQRS"
                     /locus_tag="WGQ68_000175"
     CDS             11572..12459
                     /gene="gluQRS"
                     /locus_tag="WGQ68_000175"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HEZ0714061.1"
                     /translation="MYTGRFAPSPTGLLHIGSLLTAVASYADARSNGGKWLVRMEDLD
                     PPREMPGAASHILHTLEAFGFEWDGEVAYQSRRYALYEETLCRLKTAGLVYPCHCSRK
                     DWQTGARRGADGFVYNGRCRNPQQRPALQGKQPAWRIRVPDRDIGFSDGIVGGYAQNL
                     ARDIGDFVLLRADGYWAYQLAVVADDAEQGVTHIVRGQDLLVSTPRQIYLQQCLDVPT
                     PQYAHLPLLTNAQGQKWSKQTLAPALDLNRREQLLRQVFRYLNLPEAPEADRPAELLD
                     WAVAHWDMGKVPKHAITAP"
     gene            complement(12529..13539)
                     /gene="dusA"
                     /locus_tag="WGQ68_000176"
     CDS             complement(12529..13539)
                     /gene="dusA"
                     /locus_tag="WGQ68_000176"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HEZ0714062.1"
                     /translation="MNDNTQTLPPRRLSVAPMLDWTDRHYRYMARQITRNTWLYSEMV
                     NAGAIVYGDKDRFLMFNEGEQPVALQLGGSDPSDLAKAAKAAEEYGYNEVNLNCGCPS
                     PRVQKGAFGACLMNEVGLVADCLNAMQDAVKIPVTVKHRIGVDRQTEYQTVADFVGTL
                     RDKTACKTFIVHARNAWLDGLSPKENRDVPPLKYDYVYRLKQEFPGLEIIINGGITTN
                     EEIAQHLQHVDGVMVGREAYHNPMVMREWDRLFYGDTRSPIEYADLVQRLYTYSQAQI
                     QAGRGTILRHIVRHSLGLMHGLKNARTWRRMLSDATLLKDNDGGLILEAWKEVERANM
                     RE"
     gene            complement(13570..14061)
                     /locus_tag="WGQ68_000177"
     CDS             complement(13570..14061)
                     /locus_tag="WGQ68_000177"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEZ0714063.1"
                     /translation="MRAVIQKTVGAKVDVVSENGTETCGKIDGGFVVLLGVTHSDTEK
                     DARYIADKIAHLRVFEDEAGKLNLSLKDVGGAVLLVSQFTLYADATSGRRPSFSQAAP
                     AEQAQQLYLRTAELLRGHGIHVETGRFRTHMQVSLCNDGPVTILLDSFMTRISPKMKV
                     VPD"
     gene            complement(14135..14893)
                     /locus_tag="WGQ68_000178"
     CDS             complement(14135..14893)
                     /locus_tag="WGQ68_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0714064.1"
                     /translation="MKQKKTAAAVIAAMLAGFAAAKAPEIDPALVDTLVAQIMQQADR
                     HAEQSQKPDGQVIRNDAVRRLQTLEVLKNRALKEGLDKDKDVQNRFKIAEASFYAEEY
                     VRFLERSETVSESALRQFYERQIRMIKLQQVSFATEEEARQAQQLLLKGLSFEGLMKR
                     YPNDEQAFDGFIMAQQLPEPLASQFAAMNRGDVTRDPVKLGERYYLFKLSEVGKNPDA
                     QPFELVRNQLEQGLRQEKARLKIDAILEENGVKP"
     gene            complement(14964..15830)
                     /locus_tag="WGQ68_000179"
     CDS             complement(14964..15830)
                     /locus_tag="WGQ68_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0714065.1"
                     /translation="MKAKILTSVALLACSGSLFAQTLATVNGQKIDSSVIDAQVAAFR
                     AENSRAEDTPQLRQSLLENEVVNTVVAQEVKRLKLDRSAEFKNALAKLRAEAKKSGDD
                     KKPSFKTVWQAVKYGLNGEAYALHIAKTQPVSEQEVKAAYDNISGFYKGTQEVQLGEI
                     LTDKEENAKKAVADLKAKKGFDAVLKQYSLNDRTKQTGAPVGYVPLKDLEQGVPPLYQ
                     AIKDLKKGEFTATPLKNGDFYGVYYVNDSREVKVPSFDEMKGQIAGNLQAERIDRAVG
                     ALLGKANIKPAK"
     gene            complement(15879..16157)
                     /locus_tag="WGQ68_000180"
     CDS             complement(15879..16157)
                     /locus_tag="WGQ68_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0714066.1"
                     /translation="MPAVDLIRERLQTLDPLVLEIGDESHLHKGHAGNTGGGHYAVLV
                     VSGRFEGVSRLNRQKTVKSLLKDLFSGGMIHALGIRAATPDEYFHTAD"
     gene            complement(16157..16447)
                     /locus_tag="WGQ68_000181"
     CDS             complement(16157..16447)
                     /locus_tag="WGQ68_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEZ0714067.1"
                     /translation="MEYFMLLATDGEDVHEARMAARPEHLKRLETLKSEGRLLTAGPN
                     PLPEDSNRVSGSLIVAQFESLDAAEAWAEDDPYVHAGVYSEVLIKPFKAVFK"
     gene            complement(16450..16980)
                     /locus_tag="WGQ68_000182"
     CDS             complement(16450..16980)
                     /locus_tag="WGQ68_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEZ0714068.1"
                     /translation="MKFVSDLLSVILFFATYTVTKNMIAATAVALVAGVVQAAFLYWK
                     YKKLDTMQWVGLVLIVVFGGATIVLGDSRFIMWKPSVLFWLGALFLWGSHLAGKNGLK
                     ASIGREIQLPDAVWAKLTYMWVGFLIFMGIANWFVFTRFESQWVNYKMFGSTALMLVF
                     FIIQGIYLSTCLKKED"
     gene            17288..19801
                     /locus_tag="WGQ68_000183"
     CDS             17288..19801
                     /locus_tag="WGQ68_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV family protein"
                     /protein_id="HEZ0714069.1"
                     /translation="MKNNRQIKLIAASVAVAASFQAHAGLGGLNIQSNLDEPFSGSIT
                     VTGEEAKALLGGGSVTVSEKGLTAKVHKLGDKAVIAVSSEQAVRDPVLVFRIGAGAQV
                     REYTAILDPVGYSPKTKSALSDGKTHRKTAPTAESQENQNAKALRKTDKKDSANAAVK
                     PAYNGKTHTVRKGETVKQIAAAIRPKHLTLEQVADALLKANPNVSAHGRLRAGSVLHI
                     PNLNRIKAEQPKPQTAKPKAETASMPSEPSKQATVEKPVEKPEAKVAAPEAKAEKPAV
                     RPEPVPAANTAASETAAESAPQEAAASAIDTPTDETGNAVSEPVEQVSAEEETESGLF
                     GGSYTLLLAGGGAALIALLLLLRLAQSKRARRTEESVPEEEPGLDDAADDGIEITFAE
                     VETPATPEPAPKNDVNDTLALDGESEEELSAKQTFDVETDTPSNRIDLDFDSLAAAQN
                     GILSGALTQDEETQKRADADWNAIESTDSVYEPETFNPYNPVEIVIDTPEPESVAQTA
                     ENKPETVDTDFSDNLPSNNHIGTEETASAKPASPSGLAGFLKASSPETILEKTVAEVQ
                     TPEELHDFLKVYETDAVAETAPETPDFNAAADDLSALLQPAEAPSVEENITETVAETP
                     DFNATADDLSALLQPSEVPAVEENAAETVADDLSALLQPAEAPAVEENVTETVAETPD
                     FNATADDLSALLQPSEAPAVEENAAETVADDLSALLQPAEAPAVEENAAEITLETPDS
                     NTSEADALPDFLKDGEEETVDWSIYLSEENIPNNADTSFPSESVGSDAPSEAKYDLAE
                     MYLEIGDRDAAAETVQKLLEEAEGDVLKRAQALAQELGI"
     gene            complement(19974..20450)
                     /gene="gloA"
                     /locus_tag="WGQ68_000184"
     CDS             complement(19974..20450)
                     /gene="gloA"
                     /locus_tag="WGQ68_000184"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEZ0714070.1"
                     /translation="MADVYAKQNRSLTTTERNTKMRLLHTMLRVGNLEKSLDFYQNVL
                     GMKLLRRKDYPEGRFTLAFVGYGDETDSTVLELTHNWDTERYDLGNAYGHIAVEVDDA
                     YEACERVKRQGGNVVREAGPMKHGTTVIAFVEDPDGYKIEFIQKKSGDDSVAYQTA"
     gene            complement(20414..21583)
                     /gene="lapB"
                     /locus_tag="WGQ68_000185"
     CDS             complement(20414..21583)
                     /gene="lapB"
                     /locus_tag="WGQ68_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HEZ0714071.1"
                     /translation="MDNELWIILLPIILLPVFFAMGWFAARVDMKTVLKQAKSIPSGF
                     YKSLDALVDRNSGRAARELAEVIDQQPQSYDLNLTLGKLYRQRGENDKAINIHRTMLD
                     SPDTVGEKRARVLFELAQNYQSAGLVDRAEQIFLGLQDGKMAREARQHLLNIYQQDRD
                     WEKAVETARLLSHDDQTYQFEIAQFYCELAQAALFKSNFDVARFNVGKALEANKKCTR
                     ANMILGDIEHRQGNFPAAVEAYAAIEQQNHAYLSMVGEKLYEAYAAQGKPEEGLNRLT
                     GYMQTFPELDLINVVYEKSLLLKCEKEAAQTAVELVRRKPDLNGVYRLLGLKLSDLDP
                     AWKADADMMRSVIGRQLQRSVMYRCRNCHFKSQVFFWHCPACNKWQTFTPNKIEV"
     gene            complement(21594..21908)
                     /locus_tag="WGQ68_000186"
     CDS             complement(21594..21908)
                     /locus_tag="WGQ68_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEZ0714072.1"
                     /translation="MKLIYTVVKIIILLFFLLLAVINTDAVTFSYLPGQKFDLPLIVV
                     LFGAFVVGIIFGMFALFGRLLSLRGENGRLRAEVKKNARLTGKELTAPPAQNAPESAK
                     QP"
     gene            22218..23216
                     /gene="ilvE"
                     /locus_tag="WGQ68_000187"
     CDS             22218..23216
                     /gene="ilvE"
                     /locus_tag="WGQ68_000187"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEZ0714073.1"
                     /translation="MSRPVPAVFGSVFHSQMPVLAYREGKWQPTEWQSSQDLSLAPGA
                     HALHYGSECFEGLKAFRQADGKIVLFRPTANIARMRQSADILHLPRPETQAYLDALVE
                     LVKRAADEIPDAPAALYLRPTLIGTDPVIGKAGSPSETALLYILASPVGDYFKVGSPV
                     KILVETEHIRCAPHMGRVKCGGNYASAMHWVLKAKAEYGANQVLFCPNGDVQETGASN
                     FILINGDEIITKPLTDEFLHGVTRDSVLTVAKDLGYTVSERNFTVAELKAAVENGAEA
                     ILTGTAAVISPVTSFVIDGKEIEVKSQERGYAIRKAITDIQYGLAEDKYGWLVEVC"
     gene            complement(23461..24246)
                     /gene="fabI"
                     /locus_tag="WGQ68_000188"
     CDS             complement(23461..24246)
                     /gene="fabI"
                     /locus_tag="WGQ68_000188"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEZ0714074.1"
                     /translation="MGFLQGKKILITGMISERSIAYGIAKACREQGAELAFTYVVDKL
                     EERVRKMAAELDSELVFRCDVASDDEINQVFADLGKHWDGLDGLVHSIGFAPKEALSG
                     DFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALSYLGAVRAIPNYNVM
                     GMAKASLEAGIRFTAACLGKEGIRCNGISAGPIKTLAASGIADFGKLLGHVAAHNPLR
                     RNVTIEEVGNTAAFLLSDLSSGITGEITYVDGGYSINALSTEG"
     gene            complement(24401..25222)
                     /gene="dapD"
                     /locus_tag="WGQ68_000189"
     CDS             complement(24401..25222)
                     /gene="dapD"
                     /locus_tag="WGQ68_000189"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEZ0714075.1"
                     /translation="MSLQNIIETAFENRADITPTTVTPEVKEAVLETIRQLDSGKLRV
                     AERLGVGEWKVNEWAKKAVLLSFRIQDNEVLNDGVNKYFDKVPTKFADWSEDEFKNAG
                     FRAVPGAVARRGSFVAKNVVLMPSYVNIGAYVDEGAMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQAAPTIIEDNCFIGARSEIVEGVIVEEGSVISMGVFIGQSTKIFDRT
                     TGEIYQGRVPTGSVVVSGSMPSKDGSHSLYCAVIVKRVDAQTRAKTSVNELLRGI"
     gene            complement(25232..25462)
                     /locus_tag="WGQ68_000190"
     CDS             complement(25232..25462)
                     /locus_tag="WGQ68_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714076.1"
                     /translation="MPSERRRRGIKVPPPFCIDSGTEKAIPAARPIPKRQCSATARVL
                     LTNMRLRGTIAHSFQMNILLENTGKRPTKHLT"
     gene            complement(25432..27075)
                     /gene="pgi"
                     /locus_tag="WGQ68_000191"
     CDS             complement(25432..27075)
                     /gene="pgi"
                     /locus_tag="WGQ68_000191"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0714077.1"
                     /translation="MNAFTRAWYALERHYQDTRHVLLRDRFACEPDRFERMHERLDGM
                     LFDYSKNRLGEDTLQLLCRLAETADLEGKMRALRTGAKVNGSEGRAALHTALRLPDGA
                     DAVYADGRDVLPEIRRELNRALEFAHSLDDGSYQGITGKRITDFVHIGIGGSDLGPAM
                     CVQALEPFRRHIAVHFAANADPACLDAVLCRLNPETTVFCVASKSFKTPETLLNAQAV
                     KAWYRGAGFSESETARHFCAVSADTEAAQSFGIAAERVFAMYDWVGGRYSVWSPVGLP
                     VMVAVGGARFRELLAGAHAMDSHFFHTPLRRNIPVLMALIAVWYNNFQHADGQTAVPY
                     SHNLRLLPAWLNQLDMESLGKSRASDGSPAVCKTGGIVFGGEGVNCQHAYFQLLHQGT
                     RLIPCDFIVPMTAQGVEDGRSRFTVANAFAQAEALMKGKTLDEARAELADLPEAERER
                     LAPHKEFPGNRPSNSILLDRLTPYNLGMLMAAYEHKTFVQGAIWNVNPFDQWGVEYGK
                     QLAKTIIGELEGGTSVHDASTEGLMAFYRECRLKGGGAA"
     gene            27585..28817
                     /gene="pilG"
                     /locus_tag="WGQ68_000192"
     CDS             27585..28817
                     /gene="pilG"
                     /locus_tag="WGQ68_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus assembly protein PilG"
                     /protein_id="HEZ0714078.1"
                     /translation="MAKNGGFSLFAKKEKRFIFEGRHSASDKLVNGEVSAFTEEEARK
                     KLAKRGIRPLQITRVKTSSKRKITQEDITVFTRQLSTMIKAGLPLMQAFEIVARGHGN
                     PSMTEMLMEIRGEVEQGSSLSRAFSNHPKYFDRFYCNLVAAGETGGVLESLLDKLAIY
                     KEKTQAIRKKVKTALTYPVSVIAVAIGLVFVMMIFVLPAFKEVYANMGAELPALTQTV
                     MDMSDFFVSYGWMVLIALGFAIYGFLKLKARSIKIQRRMDAILLRMPIFGDIVRKGTI
                     ARWGRTTATLIAAGVPLVDVLDSTAGAAGNLIYEEATREIRTRVIQGLSMTSGMRATE
                     LFPNMMLQMSSIGEESGSLDDMLNKAAEFYEDEVDNAVGRLSAMMEPIIIVILGLVIG
                     TLLVAMYLPLFNLGNVVA"
     gene            28891..29751
                     /locus_tag="WGQ68_000193"
     CDS             28891..29751
                     /locus_tag="WGQ68_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEZ0714079.1"
                     /translation="MSDLSVLSPFAVPLAAVFGLLVGSFLNVVIYRVPVMMERGWTVF
                     AKEYLNLPLTEEESRTFNLMKPDSCCPKCRVPIRAWQNIPIVSYLLLRGKCASCQTKI
                     SIRYPLIELLTGVLFGLVAWQYGWSWITLGGLVLTAFLISLTFIDADTQYLPDSMTLP
                     LIWLGLIFNLDGGFVPLQSAVLGAVAGYGSLWLLCAVYKLLTGKTGMGNGDFKLIAAL
                     GAWLGISALPVLIFVSSLIGLVAAIVMRVAKGQHFAFGPALTVSGWIIFTANDSVWRA
                     VNWWLTHPVL"
     gene            29753..30385
                     /locus_tag="WGQ68_000194"
     CDS             29753..30385
                     /locus_tag="WGQ68_000194"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEZ0714080.1"
                     /translation="MTVWVGLTGGIGSGKSAAAQCFADLGVPRIDADAAAHSLTASDG
                     IALPEIRRLFGDTVFDTQGLLRRDILRKEVFASPSRKALLESVMLPLIFSEIKKQQET
                     FTDAAYGIVEIPLLTEKRQFISLIRRVLTISAPLEKRIGRVMARSGLTRGEVAAVISH
                     QASESERLLLADDVLLNDGSLKSLREKTMRLHAFYSGIFASKPTQGKHNG"
     gene            30378..30587
                     /gene="yacG"
                     /locus_tag="WGQ68_000195"
     CDS             30378..30587
                     /gene="yacG"
                     /locus_tag="WGQ68_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEZ0714081.1"
                     /translation="MAESRQTRLQVKCPTCQTAVVWKPENAFRPFCSQRCKLIDLGGW
                     ADEEYTVTAQEEGLLEISEPDGAYR"
     gene            complement(30672..32348)
                     /gene="pilB"
                     /locus_tag="WGQ68_000196"
     CDS             complement(30672..32348)
                     /gene="pilB"
                     /locus_tag="WGQ68_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEZ0714082.1"
                     /translation="MSVGLLRILVQNQVVTVEQAEHYYNESQAGKEVLPMLFSDGVIS
                     PKSLAALIARVFSYSILDLRHYPRHRVLMGVLTEEQMVEFHCVPVFRRGDKVFFAVSD
                     PTQMPQIQKTVSAAGIEVELVIVEDDQLAGLLDWVGSRSTSLLQELGEGQEEEESHTL
                     YIDNEEAEDGPVPRFIHKTLSDALRSGASDIHFEFYEHNARIRFRVDGQLREVVQPPI
                     AVRGQLASRIKVMSRLDISEKRIPQDGRMQLTFQKGGKPVDFRVSTLPTLFGEKVVMR
                     ILNSDAASLNIDQLGFEPFQKKLLLEAIHRPYGMVLVTGPTGSGKTVSLYTCLNILNT
                     ESVNIATAEDPAEINLPGINQVNVNDKQGLTFAAALKSFLRQDPDIIMVGEIRDLETA
                     DIAIKAAQTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVSLIMAQRLLRRLCSS
                     CKQEVERPSASALKEVGFTDEDLAKDWKLYRAVGCDRCRGQGYKGRAGVYEVMPISEE
                     MQRVIMNNGTEVDILDVAYKEGMVDLRRAGILKVMQGITSLEEVTANTND"
     gene            complement(32489..32551)
                     /locus_tag="WGQ68_000197"
                     /pseudo
     CDS             complement(32489..32551)
                     /locus_tag="WGQ68_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(32749..32820)
                     /locus_tag="WGQ68_000198"
     tRNA            complement(32749..32820)
                     /locus_tag="WGQ68_000198"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32785..32787),aa:Arg,seq:cct)
     gene            complement(32840..33286)
                     /locus_tag="WGQ68_000199"
     CDS             complement(32840..33286)
                     /locus_tag="WGQ68_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEZ0714083.1"
                     /translation="MNVSFAPLFLVTLILLGVVSNNNSITISATILLLMQQTALIQFV
                     PLVEKHGLNLGIILLTIGVLSPLVSGKAQVPPVAEFLNFKMISAVFIGIFVAWLAGRG
                     VPLMGQQPVLITGLLIGTVIGVAFMGGIPVGPLIAAGILSFVVGKG"
     gene            complement(33291..34265)
                     /gene="ispB"
                     /locus_tag="WGQ68_000200"
     CDS             complement(33291..34265)
                     /gene="ispB"
                     /locus_tag="WGQ68_000200"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HEZ0714084.1"
                     /translation="MLENLPYFQRHLPEDLAKVNEVINRAVQSDVALISQIGTYIISA
                     GGKRLRPIMTILAGKAVGYDDEKLYSLAAMVEFIHTSTLLHDDVVDESDLRRGRATAN
                     NLFGNAAAVLVGDFLYTRAFQLMVASGSMRVLEVMADATNIIAEGEVMQLMNIGNTDI
                     TEEQYIQVIQYKTAKLFEAAAQVGAILGKASPEHERALKDYGMYVGTAFQIIDDVLDY
                     SGETEETGKNVGDDLAEGKPTLPLIYLMRQGSEQVANDVRTALENADRSYFEKIHDYV
                     VRSDALAYSIGEARKAVDCAVTALDALPDSEVKDAMIQLAKESLVRVS"
     gene            complement(34281..34469)
                     /locus_tag="WGQ68_000201"
     CDS             complement(34281..34469)
                     /locus_tag="WGQ68_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714085.1"
                     /translation="MVNIRTPLCGTRHCIVICLFCQSLRGSDNHMPSEKMYPDGFADI
                     IARYLNQLSSGICRLTET"
     gene            34489..34797
                     /gene="rplU"
                     /locus_tag="WGQ68_000202"
     CDS             34489..34797
                     /gene="rplU"
                     /locus_tag="WGQ68_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEZ0714086.1"
                     /translation="MYAVVKTGGKQYKVSVGEKLKVEQIPAELDSQIELTEVLMIADG
                     ESVKVGAPFIEGAKVTAKVVAHGRGEKVRIFKMRRRKHYQKRQGHRQNFTQIEIVAIA
                     "
     gene            34822..35094
                     /gene="rpmA"
                     /locus_tag="WGQ68_000203"
     CDS             34822..35094
                     /gene="rpmA"
                     /locus_tag="WGQ68_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEZ0714087.1"
                     /translation="MASKKAGGSTRNGRDSEAKRLGVKAYGNELIPAGSIIVRQRGTK
                     FHAGDNVGMGKDHTLFAKVDGYVEFKTKGALNRKTVSIRPYTGSEE"
     gene            35483..36667
                     /gene="ubiM"
                     /locus_tag="WGQ68_000204"
     CDS             35483..36667
                     /gene="ubiM"
                     /locus_tag="WGQ68_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-demethoxyubiquinol-8 5-hydroxylase UbiM"
                     /protein_id="HEZ0714088.1"
                     /translation="MSLHSDILVVGAGPAGLSFAAELAGSGLKVTLIERSPLTVLQNP
                     PYDGREIALTHFSREIMQRLGMWDKIPENEIYPLRDAKVLNGRSDYQLHFPQPTEARG
                     EPADCLGYLISNHNIRRAAYEVVSQLDNITILTDTVVKEVKTSDNEAQVILENGKILT
                     ARLLLAADSRFSQTRRQLGISSDMHDYSRTMFVCRMKHTLSNQHTAYECFHYGRTIAL
                     LPLEEHLTNTVITVDTDKINSVQNLSPEELAASVKEQLKGRLGDMELVSSIHHYPLVG
                     MIAKRFYGKRSALIGDAAVGMHPVTAHGFNLGLSSADILAKLILEAEQRGQDIGAASL
                     LEKYSSKHMLHAQPLYHGTNMMLKLFTNETAPAKLLRGLVLRAGNNFPPLKKLITKQL
                     TG"
     gene            complement(36938..37093)
                     /gene="rpmG"
                     /locus_tag="WGQ68_000205"
     CDS             complement(36938..37093)
                     /gene="rpmG"
                     /locus_tag="WGQ68_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009766925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEZ0714089.1"
                     /translation="MRDKIKLESSAGTGHFYTTTKNKRTMPGKLEIKKFDPVARKHVV
                     YKETKLK"
     gene            complement(37121..37354)
                     /gene="rpmB"
                     /locus_tag="WGQ68_000206"
     CDS             complement(37121..37354)
                     /gene="rpmB"
                     /locus_tag="WGQ68_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007586227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEZ0714090.1"
                     /translation="MARVCKVTGKRPMSGNNVSHANNKTKRRFLPNLQSRRFWVESEN
                     RWVRLRVSNAALRTIDKVGIDVVLADLRARGEA"
     gene            complement(37604..39130)
                     /gene="farB"
                     /locus_tag="WGQ68_000207"
     CDS             complement(37604..39130)
                     /gene="farB"
                     /locus_tag="WGQ68_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     permease subunit FarB"
                     /protein_id="HEZ0714091.1"
                     /translation="MDYPPLKGAALAWVTLSLGLAVFMEVLDTTIANVAVPVIAGNLG
                     AATTQGTWVITSFAVANAVSVPLTGFLAKRIGEVKLFTAAAVGFVITSWLCGIAPNLQ
                     ALVFFRILQGFIAGPLIPLSQSLLMASYPPAKRTLALALWAMTVVVAPVLGPIFGGWI
                     SGNWHWGWIFFINIPIGIISAWITWKHLKYRETETVKMPTDYVGLTLMVVGIGALQMM
                     LDRGKELDWFASGEIITLGVTALVCLSYFIVWELGEKYPIVDLSLFKDRNFTVGIIAT
                     SLGFMVYMGTLTLLPLVLQTNLGYTSTWAGLAAAPVGILPVFLSPLIGRFGNKIDMRL
                     LVTASFLTFAFTFYWRTDFYADMDIGNVIWPQFWQGVGVAMFFLPLTTITLSHMKGGQ
                     IAAAGSLSNFLRVLMGGVGVSVVSTLWERREALHHTRFAEHITPYSATLHETAAHLSQ
                     HGVSDIQTLGIINNTITQQGFIIGSNEIFMAGSLLFIIMIPVIWLAKPPFHNGGGGGH
                     "
     gene            complement(39154..40308)
                     /gene="farA"
                     /locus_tag="WGQ68_000208"
     CDS             complement(39154..40308)
                     /gene="farA"
                     /locus_tag="WGQ68_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid resistance MFS efflux transporter
                     adaptor subunit FarA"
                     /protein_id="HEZ0714092.1"
                     /translation="MDTHTDETKLQNTQAKRKRCLTALTLLFALAAAAAGSAFFLWWQ
                     HEEETEDAYVAGRVVQVTPQKGGTVRKVLHDDTDAVKKGDVLAVLDDDNDVLAYERAK
                     NELIQAVRQNRRQNAATSQAGAQVALRRADLARAQDDLRRRSALAESGAVSAEELAHA
                     RAAVSQAQAAVKAALAEESSARAALGGQVSLREQPAVQTAIGRLKDAWLNLQRTQIRA
                     PADGQVAKRSVQVGQQVAAGAPLMAVVPLSDVWVDANFKETQLRHMKIGQPAELVSDL
                     YGKQIVYRGRVAGFSAGTGSAFSLIPAQNATGNWIKVVQRVPVRIVLNREDVDRHPLR
                     IGLSMTVKVDTSAAGAPVSKTPGAALPEMESTDWSEVDRTVDEILGQSAP"
     regulatory      40701..40745
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            40751..41224
                     /gene="queF"
                     /locus_tag="WGQ68_000209"
     CDS             40751..41224
                     /gene="queF"
                     /locus_tag="WGQ68_000209"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEZ0714093.1"
                     /translation="MSRNTEELQGISLLGNQKTQYPIGYAPEILEAFDNKHPDNDYFV
                     KFVCPEFTSLCPMTGQPDFATIVIRYIPHIKMVESKSLKLYLFSFRNHGDFHEDCVNI
                     IMKDLIALMDPKYIEVFGEFTPRGGIAIHPFANYGKAGTEFEALARKRLFEHDAQ"
     gene            41298..41984
                     /locus_tag="WGQ68_000210"
     CDS             41298..41984
                     /locus_tag="WGQ68_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HEZ0714094.1"
                     /translation="MYAFTDAQQQKALFWLVLFHILIIAASNYLVQFPFQIFGIHTTW
                     GAFSFPFIFLATDLTVRIFGSHLARRIIFWVMFPALLLSYVFSVLFHDGGWTGLGALS
                     EFNTFVGRIALASFAAYALGQILDIFVFDKLRRLKAWWVAPTASTVIGNALDTLVFFA
                     VAFYASSDGFMAANWQGIAFVDYLFKLTVCGLFFLPAYGVILNLLTKKLTTLQTKQAQ
                     DRPAPSLQNP"
BASE COUNT        10177 a        11874 c        10537 g         9682 t
ORIGIN      
        1 agatatacgg gaaggacagg gcatcagcat atatttcagc ccgaagccgt ttcattcgtt
       61 taaaaaactg cttttttcac aatttacctt tacaaagcct atacgtatac ttacaatata
      121 aaacaaacaa atatagtgga ttaacaaaaa ccagtacggc gttgtctcgc cttgccgtac
      181 tatctgtact gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataagtt
      241 gacatattgt gcgatgtcga gggctgtacc gtattttccc atcgtatttt cccgttcttt
      301 atatgagcgg caggcatcct cataaaagaa gggataccga taaggaaaaa taccccggca
      361 taaaaaatat actgattaat caatacaaat ccaacaaaca gttcatcgta ttcttttctg
      421 ttggcgtata aaaaatactg atatatgcct tcactttcat agggcgacgc tttatgtacc
      481 gtcctgtgtg ttgaaacgtc ggttttcctt accgcctgcc ccgcttttcc ctgattgctg
      541 ccaactgccg tccgaacctg aaccgtcagg tttcagacgg cattttttct gcctgctccc
      601 acaattgccg tttttgtgcg gcggtcagtt ttttgacggc gatttcctgc ctgagtgccg
      661 ttcctttatc catcccgcct gcaacgatac gcatcgccac cggtttgaat acgcgggtat
      721 atttcgcgcc tttaccggtt gtgtgggcgg caagccgctg ttgcggattc gggctgatgc
      781 cgcaatagaa cgcgctgttt tcacacaata tcaggtaaac gctccagttt gacgcgttca
      841 tctgcctgca tcctgagttt tctccccgtt ccaaggcgca actttcagca gcagcagcat
      901 acccggtagg gcgagtgcga aacacagttg gaaaaacggt acataaccga gccattcaat
      961 cagataaccg gcaaaggaat tgatgaccgt gcgcgggacg gcggacaggc tggtaaacag
     1021 cgcaagctgc gttgcggtaa atgcgggatt ggtttcacgc gccatatacg atacgaacgc
     1081 cgccgtcccc aaccccacgc cgaccgcttc cgcgccgata actgccgcca gcatcagcct
     1141 ctcgcctgtg ccgaccgtgt cgaaaggtcc gaaccctgcc agccatacaa accccaaaac
     1201 ggttacagcc tgcaccgcgc cgaatagcca caaggctttg tttacgccga ttttcagcat
     1261 ccacacaccg cccaagatac ctgccgccac tgccggccac agtcctgcat ttttcgcaat
     1321 caaaccgatg tcggtcttgc tgaaacccat atccagataa aacggcgttg ccaacgcggt
     1381 tgccatactg tcgccgagtt tgtaaaggaa gataaacagc agcacgcaca ccgccgaagc
     1441 gatgcccttg cgcgtaaaaa attctttaaa cggctctacc acggtctgct tcaacgtttt
     1501 aggaacggca ggcggcaaca cgggttcgcg cgcgagaaac agcgtcatca gaaggccggg
     1561 cagcataaat aatgaagtaa taacaaatac ttctgaccac ggcatcctgt ctgccaacac
     1621 caaactcaat gaaccgggaa tcagggcggc aatccggtag gcgttcacat gaaccgagtt
     1681 gcccaaaccc aattcttcgt ccgacaaaat ctcgcgcctg aacgcatcca atacaatatc
     1741 ctgactggcg gaaaaaaaag cgacaagcac cgacaagccg gcaatcagcg gcagatgatt
     1801 acgggggttt aaaaaggcat aggccgccaa agccgccagc aaccctgcct gcgtcagcag
     1861 catccacccg cgccgccgtc ccaaaacggg cagcctgacc gcgtccatca gcggcgacca
     1921 caaaaatttc caagtaaacg gcagaccgat taacgccatc agcccgatgc tcttcaaatc
     1981 cacctgctcg ctgcgcaacc acgccggaat caggttaatc agaaagtaca gcggcagccc
     2041 cgaggtaaac cccgtaaaaa tacagacgag catattgcgg gaaaagattt gcccgataaa
     2101 acctgatttc gatgcagtca tcgttcgtgc agccaaaaaa caaaagccta ttgtagcaaa
     2161 tatcgcccgc ctgctgaaaa acgccgcgcc gccgttttgc cgttccaatg caaaatgccg
     2221 tctgaaactg tgttcagacg gcattgcttt tctgaaaacc cgacgtgctt acaggctgta
     2281 atacatttca aattccagcg gatgcggcgc catgcggatg cggcggacat cttcctcttt
     2341 aaaggcgatg tagctgtcga tccagtcttt gctgaacacg ccgccgcgca gcaggaattc
     2401 gtggtcggct ttgagggcgg cgagtgcttc ttccaaagaa gcgcaaacgg tcgggacgag
     2461 cgcgtcttct tccggcggca ggtcgtagag gtttttatcg gcaggatcgc ccggatggat
     2521 tttgttttga ataccgtcca aaccggccat caacaaagcg gcaaatgcca agtatgggtt
     2581 ggcggtcgga tcggggaaac gcgcttcgat gcggcgcgcc ttgctgctgt tcacggacgg
     2641 aatgcggatg gaagcggaac ggtttttggc ggagtatgcc agtttggtcg gcgcttcaaa
     2701 gtgcggcacg aggcgtttgt aggagttggt ggacggattg gtaatcgcgt tcaaggcttt
     2761 ggcgtgtttg atgatgccgc cgatgtagta gagcgcggtg tcgctcaagc cggcatagcc
     2821 gtcgcctgcg aacaggtttt gaccgtcttt ccaaatggat tggtgaacgt gcataccgct
     2881 gccgttgtcg cccataatcg gtttgggcat aaaggtggcg gttttgccga agttgtgggc
     2941 aacgttttga atcacatatt tcatgtcttg ggtttggtcg gcgcgtttga ccaaggtggc
     3001 gaagcgcgtg ccgatttcca tttggctgcc ggtgccgact tcgctgtggt ggacttcgac
     3061 ttcgatgccg agttcttcca aaatgttcac catggcggag cgcaaatctt ggccgcaatc
     3121 aatcggcgcg acgggcgcgt aaccgccttt aacggcgggg cggtggccgg tgttttgacc
     3181 gtccatatgc agaccgcttg cccatgcgcc gctttcggac gtaatttcgt aacgggtttt
     3241 gtgcatatcg gtttcaaatt ctacgccgtc aaaaacgaag aattcaggct cagggccgaa
     3301 ataggcggtg tcgccgatac cggaagattt caaataggct tcggcgcggc gtgcgataga
     3361 gcgggggtcg cggtcatagc cttggccgtc agccgggtcg ataacgtcgc aggtaaagac
     3421 aacggtggta tcgtcgtaga aaggatcgac gaaggctgta gacgcatcgg gacgcaactg
     3481 catatctgaa gcctgaatac ctttccaacc gccgatggac gaaccgtcaa acgcctgtcc
     3541 gttttcaaac cactcttcgg ggtcttccaa cacgatgcgc gcaggcacgg taaagtggtg
     3601 ctgcttgcct ttggtatcgg taaagcgcaa atcgacaaag cgggcttcgc tttcttcaat
     3661 caattttacg gcgtttttaa tagacatttt tcagctcctg gaaaagggaa atagcaaaat
     3721 atgcggaacg cggcagatta gctcaaagcc agcatgattc taccacgtcc atgccgtctg
     3781 aaactggccc tgcgtcaaat tccaagctgt aaaatatagt ttcagacggc aatattgtca
     3841 acaatatgct tcggtttgcc cgttcccaag cagaatgccc gcccttttat ccgaatgccg
     3901 acaaaacaga tgccgtctga aagcagttca gacggcatct gttgtttggt ttgaaaacgg
     3961 tcagcgagga tagccgttgc agacaaattt gtactgagcc tctccaagcg taccgggaat
     4021 aacataattc cattcggcat aggatggtgt ataagaagcg atagagtttc ccgatttgga
     4081 ataccacgtt gccgtcaata tcctaaaacg tttaccactg caatccattt cttccaacgt
     4141 tttagctgta ttaaaaaatt ttccattgcc atacttttgc ggcttggcga accttgtttc
     4201 aaaccatgca ctgttgtccg tacggctgtc tgcatcacca aacgtggcag tattactatc
     4261 taccaatacc caatccgccg ccaccgccgg cgttgccaac cccaatgcaa atactgccaa
     4321 gcacaacatc tttttcatgt ccgaccctct cgtccttgtc aacaaaataa agttaaattg
     4381 taaaaaaaac gacttttttc aagagaaaaa tattccgtca tgctatctga caaccgtttc
     4441 agacggcatc gtttccatct gtacgccgcc gttataaaat agccttcccg tcttttggaa
     4501 cacgaccatg accgccctcc cccgttactc cgttttcggc aaccccgttg cacacagtaa
     4561 atcgccgcaa atccatcagc aatttgccct tcaggaaggc gttgacattg aatacgaacg
     4621 catttgcgcc gacatcggcg gtttcgcgca ggcggtttcg acattttttg gaacaggcgg
     4681 ttgcggggca aacgttaccg taccgttcaa acaggaagcg tttcatctgg cggacgagca
     4741 ttccgaccgc gctttggcag caggtgcggt caatacgctg attctgttga aaaacggcaa
     4801 gctgcgtggc gacaataccg acggtatcgg tttggccaac gacatcacgc aggtcaaaaa
     4861 cattgccatc gagggcaaaa ccattttgct tttgggcgca ggcggcgcgg tgcgcggcgt
     4921 gattccggtt ttgaaagaac accgccctgc ccgtatcgtc attgccaacc gtacccgcgc
     4981 caaagccgag gaattggcgc agcttttcgg cattgaagcc gtcccgatgg cggatgtgaa
     5041 cggcggtttt gatatcatca tcaacggcac gtccggcggt ctaaacggtc agattcccga
     5101 tattccgccc gatatttttc aaaactgcgc gcttgcctac gatatggtgt acggctgcgc
     5161 ggcaaaaccg tttttagatt ttgcacgaca atcgggtgcg aaaaaaactg ccgacggact
     5221 gggtatgcta gtcggtcaag cggcggcttc ctacgccctc tggcgcggat ttacgcccga
     5281 tatccgccct gttatcgaat acatgaaagc catgtaatat gttccgcatc gtcaaatggc
     5341 tgattgccct gcccgtcggc atctttatct ttttcaatgc ctatgtgtac ggcaacatca
     5401 ttacctaccg cgccgtcgcg ccccatcgga ctgcctttat gtcgatgcgg atgaagcagt
     5461 ttgaacagga aggtcgcgat gtcgcactgg attaccgctg ggtgccctac aaccgcatct
     5521 cggtcaacct gaaaaaagcc ctgattgctt ccgaagatgc ccgtttcgcc ggacacggcg
     5581 gcttcgattg gggtggcatt caaaacgcca tcaggcgcaa ccggaacagc ggcaaagtga
     5641 aggcgggcgg ttcgaccatc agccagcagc ttgccaaaaa cctgttttta aacgaaagcc
     5701 gcagctatat ccgcaaaggc gaagaagcgg cgattaccgc gatgatggaa gccgttaccg
     5761 acaaagacag gattttcgaa ctgtatctga attcgattga atggcactac ggggtgtttg
     5821 gcgcggaagc cgcgtcccgg tatttttatc aaatacccgc cgccaaactg agcaaacagc
     5881 aggcggcgaa actgacggca cgcgtccccg ccccgctcta ctacgccgac catccgaaaa
     5941 gcaaacggct gcgcaacaaa accaatatcg tgctcagacg catgggttcg gcagagttgc
     6001 ctgaaagcga cacggactga gtgccgccgc aatataaaat gccgtctgaa atatcattca
     6061 gacggcatat gctttctgcc tttaccctct tctccaaaac gcaacggggc ggtcattatc
     6121 atttttgccc ttctacataa cggtcagaca caaaacgtgt ccgacacgga caacttccca
     6181 aaaacggcag acaaaaaaat aaccgcttat cagcggtttt ttggtggcca gaggcggaat
     6241 cgaaccgccg acacacggat tttcaatccg ttgctctacc aactgagcta tctagccttt
     6301 atctgtctcg ttaagagatg cgtattaaac caaatttcgg gacaacacgc aagcggattt
     6361 gtcgatattt tttatccaac cgactaagtc gatgttttaa gtttaaatta atttcacaga
     6421 aaaatagtgc aaatcggttt tttgccgttt gttcggcgat attctgcttt tcagacggca
     6481 aaacgcttca atatttgaag ttctcgccca aatagacggc gcgcacctgt tcgtttccga
     6541 ccaaatcatc aggttttccc gatgccaaca ccgtgccgtc tgaaataatg taggcccgat
     6601 cgcagatgct gagggtttcg cgtacgttgt ggtcggtaat taatacgccg ataccgcgcg
     6661 acttgagaaa tccgatgatt ttttggatgt cgatgacggc gatgggatcg acgccggcaa
     6721 aaggctcgtc caacaaaata aaatgcggtt tcatggcaag tacgcgggcg atttcgacgc
     6781 gtcggcgttc gccgcccgac aaagatgggg caggattatt gcgcaaacgc tcgatattca
     6841 ggtcggcaag cagtttttca acttcgccgt cgatgcggct tttgtctttg gtactgattt
     6901 ctaaaatggc gcggatgttt tgttcgacgg tcattttacg gaaaatagag gcttcctgcg
     6961 gtaggtagcc gacacccagg cgggcgcgtt cgtgtatggg caggtgacgc aattcctgcc
     7021 cgtccagcat cacgctgccc gcatcggcgg caatgagtcc aacaatcatg tagaagctgg
     7081 tggttttacc cgcaccgttg ggcccgagca ggccgacgac ttccccgctt tcgatttcaa
     7141 gggagaagct tttaacgact tggcgttttt taaaactctt ttgcaggttt tgaacaacaa
     7201 ggcggctgac gtttgcgctc ataaatatct cttcaatatc ttcggtcggc atatgccgtc
     7261 ggaaccgggt ttatgtttca gacggcattt tgggattatt cggatttttg cgtactcgaa
     7321 ggctggatga tgacgctgac cctgccggat ttggaagcgg attttgcgcc ggattttgta
     7381 ctgccgctga tggtatagac ttcggttttg gtgttgtatg taatcaccgc gccttcggcg
     7441 acatcgccgc cgcgctgtac tttggcatta ccggttaaga ctacggtgct gcctgcagac
     7501 gaataagcaa cgttgtttgc ctgaccgcgc accgtccctt tgcccccgtc caacgtttgg
     7561 ctgaagcgta cgggcgagcc gtcggccttc ataaactgtt cgcctttgtc attacgcgtt
     7621 acgttgacgc gggcggcgga aatattgagc gtaccctgtc tgatgacaac gtttccgcta
     7681 aatgtagtgc tttggttggc ttgattgaga gaaccttggt cggcctcaat ctgaataggc
     7741 tgcctgctgt cgctttgaag ggcaaaagcg ggggacgccg aaaaaaatgc tattaaaaca
     7801 aatagcttac atatcttttg tatcataaat cgtggctttc actttagatg agaagttcaa
     7861 catgcctgtt ttgtggtcgt aagtcatgcc gcccgcctga ccgtgcgatg caccatattg
     7921 gaaactgaca ggcgtatcgg tttgggcata ttgagattcg gtatcgacgt gcagcttttc
     7981 ggcttcaact ttacccgcct gccgtttgcc gtcggcggtt ttggtcagca caacgttgtt
     8041 tttaaaaaga acctgtttgt tttcggtatg gtaaacggct tcatcgctgc cgacttcgta
     8101 caacaacctg ccttcttgga agaacacgag atgcggtgaa tcgaaatgaa tgtcgctgct
     8161 ttcgggaaac tgtttcgcgc ctttcgcgct caaatgttct tttaagtatc cctgttcgtc
     8221 aaaccgcctg ccatccaagc cgtccattgt gtattgcggt tcgtcgggat tgagcctgac
     8281 ttcttcaatc tcgacttcgc tgatgcgtcc caaccatgcc gacagaccgc ccaaggcaac
     8341 cgccaatatc aatgggaacg caattccgta ccgccatctt attttcattt gatgtactcg
     8401 ttcaaagccg cgcccaaagt cccttgcgcc tgcataatca ggtcgcacac ttcgcgcacc
     8461 gcgcccgcac cgccggggcg tttggcaata taggcggcgt gttgcagcgt aaaccaatgc
     8521 gcgtcgggaa cggcaaccgg caatccgcaa cgcaccatca ccggcaaatc gaccacgtcg
     8581 tcgccgacaa aggcacactc ggcttcttcc acgccgacct gagcgcgcag ctcttcatac
     8641 gcggcgcgtt tgtccgaaat gcctttgaag tagtaattta tgcccaactg tttgacgcgg
     8701 atgccgacgg agggcgcgtc ccgacctgtg ataatcgcag tctgcacacc gcttgcctga
     8761 agcattttca gaccgtgtcc gtccagtgtg tgaaacgatt tgatttcttc gccgttatcg
     8821 cggataaaga tgcgcccgtc ggtcaaaaca ccgtccacat ccaggatcag cagtttgatt
     8881 ttggcggcgc gcgcctgtaa ttcaggagaa attgcctgca tgggatttcc ttgttttcag
     8941 acggcatatc ttaccacaaa taccgtctga aacggctgca acgccttata caatccgcgc
     9001 cgccaaaagg tcgtgcatat tcagtgcgcc gatcagcacg ccatctgcat cggtaaccag
     9061 aagcccgttc acatggtttg cctgcatgac tttcagggct tcggtggcga ggcgttcggc
     9121 ggcgatggtt ttagggtgtg tatgcatgac ttcgtctatc gaaagaccgg taaaattgtc
     9181 gcattcttga aacaggcggc gcaaatcgcc gtcggtgaat acgcctttca gacggccttg
     9241 cccgtccgtt accgccagca tccccagccc tttttcactc atgctgacga tggcttcttt
     9301 caagggcgtg ccgagtcgga cggcaggcag gccgccgcct ttgtgcataa tgtcggcaac
     9361 gcgcaaaagt aggcgtttgc cgaggctgcc ggcagggtgg ctcaaggcga agtcgtcggg
     9421 cgtgaacgcg cgcgcgcgca acagaacgac cgccaacgca tcgcccaaag ccatgacggc
     9481 ggtggtgctg gtggtcgggg caagccccag cgggcaggct tcttgcgaaa ccgatgcggt
     9541 gatatggatg tcggcatggc gcgccatggt tgaatcgggg cgggcggtga tgcagacaag
     9601 cgtgatgttt ttgcgtttga gtgcggggat gatggcggcg atttcgtcgc tttcgccgga
     9661 attggaaatc gcgacgacca cgtcgttgtc cacaatcata cccaaatcgc cgtgtgccgc
     9721 ttccgcaggg tggacgaaaa acgcaggcgt gccggtcgag gccatagttg ccgccatttt
     9781 gcgcccgata tgtcccgact tgcccatgcc cgtgataacg accctgcctt tgcagtgcaa
     9841 caacgcgtcc gccgcaaggg cgaagttttc gtccaaatcc gccgcaattt cgcgcaagcc
     9901 ttccgcttcg gtgtgcaaca cttcgcgcgc ccaatcgaga tattttccgt tttctgccat
     9961 tgccatccgc ctttgctgta acaaaactac ccaaaatttc cgaatcgggg tagcataagc
    10021 attcaaaccc cgccatccat ccaaaggaat cagaaatgac tattttatcg gacgttaaag
    10081 cattaggaca acaaatctgg ctggacaacc tttcccgctc gctggtgcaa agcggcgaat
    10141 tggcgcaaat gttgaaacaa ggcgtgtgcg gcgtaacttc caatcccgcc attttccaaa
    10201 aagccttcgc cggcgacgcg ctttatgccg acgaggttgc cgccctcaag cagcaagacc
    10261 tcagccccaa acaacgctac gaaacaatgg cggttgccga tgtgcgtgcc gcctgcggca
    10321 tctgtcttgc cgaacacgaa tccaccggcg gcaaaaccgg tttcgtcagc ctcgaagttt
    10381 cgcccgaatt gtccaaagac gcgcaaggca cggtagaaga agcgcgccgc ctctacgccg
    10441 ccatcgggtg caaaaacgcg atgattaaag tgcctgccac cgacgcaggc atcgatgcgc
    10501 tcgaaaccct cgtttcagac ggcatcagcg tgaacctgac cctgctgttc tcacgcgtcc
    10561 aaaccctcaa agcctacgcc gcctacgcgc gcggcattgc caaacgcttg gcagccggac
    10621 aaagcgttgc ccatatccac gtcgtcgcca gcttcttcat ctcccgtgtg gacggcgcgc
    10681 tggacacaac gctgcccgac cacctcaaag gcaaaatcgc catcgccctt gccaaagccg
    10741 cctatcaaga ttgggaacaa tatttcacca cccccgaatt tgccgcactg gaagcccaag
    10801 gcgcaaaccg cgtgcagctt ttatgggcat ctaccggcgt gaaaaaccca gcctatcccg
    10861 acacgctcta cgttgacaac ctgatcggcg cgcacaccgt caacaccgtt cccgatgcca
    10921 cgctcaaagc ctttatcgac cacggcacag ccaaagccac gctgaccgaa ggcgtagaag
    10981 aagcacaagc gcagcttgcc gaaaccgccg cgctcggcat agacgtagaa accctcgcca
    11041 accgcttgca ggaagacggt ttgaaacaat ttgaagaagc gtttgaaaaa ctgctcgcac
    11101 cgttggttta aacttttcat ccccgatgcc gtctgaaagg ctttcagacg gcatcgccct
    11161 tttgaccgga cacagtgcgg catatcaagg attcgcccta aaaatattaa tcaacaagaa
    11221 gttaatgcac gacattaccc actaacacaa aaggaaatag aatcatggca aaagcactcg
    11281 aaatcatttc acccaatgaa atttattcag atctgatttt taaggatccg gtacctcccc
    11341 atactatata tagagaattg atgaaactgg ttagtagaaa tgcaagtaat gagagaatcg
    11401 cagatagagc cttcgatttt ttctcgtccg gccttatgga tggttcagct accgaagagc
    11461 aatataatgc cctatatgat ttaaccttgc ttgaagagcc cgggatggaa ctgaataaag
    11521 atgagataat ggctttgatt aatagtctta aataaagggg ctgtaccaga tatgtacaca
    11581 ggacgttttg cccccagccc gaccgggctg ctccacatcg gctcgctgct gaccgccgtc
    11641 gcttcctatg ccgatgcacg ctcaaacggc ggcaaatggc tggtccgcat ggaagacctc
    11701 gatccgccgc gcgaaatgcc gggggcggca agccatatcc tgcacacgct tgaggcattc
    11761 ggattcgagt gggacggaga agtcgcctat cagagccgcc gttacgccct gtatgaagaa
    11821 acactatgcc gcctgaaaac cgccggactg gtctatccct gccattgcag ccgcaaagac
    11881 tggcagaccg gggcaaggcg gggcgcagac gggttcgtct ataacggacg ctgccgcaat
    11941 ccgcagcaac gccctgcact gcaaggcaaa cagccggcgt ggcgcatccg cgtccccgac
    12001 cgcgacatcg gcttttcaga cggcatcgtc ggcggttacg cccaaaacct cgcccgcgac
    12061 atcggcgatt tcgtcctgct tcgtgcagac ggttactggg cataccaact cgccgtcgtt
    12121 gccgacgatg ccgaacaggg cgttacccac atcgtccgcg gacaagacct gcttgtttct
    12181 acgccgcgcc aaatctattt gcagcagtgt ttggacgttc cgacaccgca atatgcccac
    12241 ctgccgctgc tgaccaacgc acaagggcag aaatggtcga aacagacgct cgcacccgca
    12301 ttggatttaa accgccgcga acaactcctc cgccaagtgt tccgttacct caacctgccc
    12361 gaagcaccgg aagccgaccg ccctgccgaa ctgctcgact gggcggtggc acactgggat
    12421 atgggcaaag tgccgaaaca cgccattacc gccccctaaa caccgcccga caaacgcaca
    12481 aatgccgtct gaaaccttca gacggcattt catacacaca gccccgccct attcgcgcat
    12541 atttgcccgt tcgacttctt tccacgcttc gagaatcagg ccgccgtcgt tgtctttcaa
    12601 gagcgttgca tcggaaagca tacgccgcca agtgcgggca tttttcagac cgtgcatcag
    12661 cccgaggctg tggcggacga tgtggcgcaa gattgtgccg cgtccggctt ggatttgggc
    12721 ttgactgtat gtgtagagac gctgcaccaa atcggcgtat tcaatcgggc tgcgggtatc
    12781 gccgtaaaac agcctgtccc attcgcgcat caccatcggg ttgtggtacg cctcgcgccc
    12841 gaccatcacg ccgtcaacgt gttgcaggtg ttgcgcgatt tcctcattgg tcgtgatgcc
    12901 gccgttgatg atgatttcca gcccgggaaa ctcttgcttg aggcggtaaa cataatcgta
    12961 tttcaagggc ggaacgtcgc ggttttcttt gggtgaaaga ccgtccagcc acgcgttacg
    13021 tgcgtggacg ataaaggttt tgcaggcggt tttgtcgcgc agcgtgccga cgaaatcggc
    13081 aacggtttgg tattcggtct gcctgtccac accgatgcgg tgtttgacgg taacgggaat
    13141 cttgaccgca tcctgcatgg cgttgaggca gtcggcaacc agcccgactt cgttcatcag
    13201 acacgcgccg aacgcgcctt tctggacgcg cggactgggg cagccgcagt tgaggttgac
    13261 ctcgttgtag ccgtattctt cggcggcttt ggcggctttc gccaaatcgg acgggtcgct
    13321 gccgcccagt tgcagggcga cgggctgctc gccttcgtta aacatcaaaa agcggtcttt
    13381 gtcgccgtaa acaatcgcgc cggcgttgac catttcgctg tacagccaag tattgcgggt
    13441 aatctggcgc gccatataac ggtagtgcct gtccgtccag tccaacatcg gggcgacgga
    13501 caggcggcgg ggaggaaggg tttgcgtgtt gtcgttcatt gtcgatattt caccattgcg
    13561 gattcaattt caatccggaa caaccttcat ttttggggaa atccgcgtca tgaaagagtc
    13621 cagcagtatg gttaccggcc cgtcgttgca gagcgacacc tgcatatgcg tgcggaaacg
    13681 ccctgtttcg acatgaattc cgtgtccgcg caacagttcc gccgttcgca ggtaaagctg
    13741 ctgcgcctgt tctgcaggtg cggcttgcga aaacgaaggc cgccgcccgc ttgtcgcgtc
    13801 ggcataaagc gtaaactgcg acaccagcag caccgcgccg ccgacatctt tcaaagacag
    13861 gttcagcttg cccgcttcgt cttcaaacac gcgcaaatgg gcgattttgt cggcgatata
    13921 gcgtgcatct ttttctgtgt cgctatgcgt tacaccgagt agcacgacga atccgccgtc
    13981 gattttgccg caggtttccg tgccgttttc ggacacgaca tccacctttg cacctaccgt
    14041 tttctgtatg accgcacgca taagccgtcc tccttacctg aacgtgcaat gccgtctgaa
    14101 aaggcttcag acggcatcgg tattggaaat gccattacgg tttgacaccg ttttcttcca
    14161 aaatggcatc gattttcaag cgggcttttt cctgtctcaa accttgttcc aactggtttc
    14221 tgaccaactc gaaaggctgc gcgtcggggt ttttcccgac ctcgctgagt ttgaacagat
    14281 aatagcgttc gcccaatttg accggatcgc gggtaacgtc gccccgattc atcgcggcaa
    14341 actgcgaagc cagcggctcg ggaagctgct gcgccataat gaaaccgtca aacgcctgct
    14401 cgtcgttcgg ataacgcttc atcagccctt caaaagacag ccctttgagc aggagctgct
    14461 gcgcctgacg cgcctcctct tcggttgcga agctgacctg ctgcaatttg atcatgcgga
    14521 tttgccgctc ataaaactga cgcagtgcgc tttcggaaac cgtttccgaa cgttccagaa
    14581 aacggacgta ctcctcggca taaaaagacg cttcggcgat tttaaagcgg ttttggacat
    14641 ccttatcctt atccaaacct tccttcaatg ccctgttttt caaaacttcc aaagtttgta
    14701 gccggcggac ggcatcgttt cggattacct gcccgtccgg tttttgggac tgctccgcat
    14761 gccggtctgc ctgctgcatg atctgcgcca ccagcgtatc caccaaagcc gggtcgattt
    14821 cgggtgcttt ggctgccgca aaacctgcca acattgcagc aataactgcg gcagcggttt
    14881 ttttctgttt catggtattc cctgtcctgc gcggcggcaa aacgcctgtc tgaacgtctt
    14941 gccgccatat cccgttttca gaattatttt gcaggtttga tgtttgcctt gcccaacagt
    15001 gcaccgacgg cacggtcaat ccgttccgcc tgaaggttgc ccgcaatctg tcctttcatt
    15061 tcatcaaaag aaggcacttt tacctcgcgg ctgtcgttga cataataaac gccgtagaaa
    15121 tcgccgtttt tcagcggcgt tgccgtaaat tcgccttttt tcaagtcctt aattgcctga
    15181 taaagcggcg gaacaccctg ttccaaatct ttcagcggca catatccgac cggcgcaccg
    15241 gtctgtttgg tacggtcgtt gagggaatat tgtttcaaga cggcatcgaa accttttttc
    15301 gccttcaagt cggcaaccgc tttttttgca ttttcttcct tgtcggtcag gatttcgccc
    15361 aactggactt cctgcgtacc tttgtaaaaa ccgctgatat tgtcatatgc ggcttttact
    15421 tcctgctcgg aaaccggttg ggttttggcg atatgcaatg cgtatgcctc gccgttcaag
    15481 ccatatttta ccgcctgcca aacggttttg aaggacggtt tcttgtcgtc gcccgacttt
    15541 ttcgcttcgg cacgcaattt ggcaagcgca tttttaaact ctgccgaccg gtcgagtttc
    15601 aggcgtttca cttcctgtgc gaccacggta ttgaccactt cgttttccag cagggattgg
    15661 cgcagttgcg gcgtgtcttc ggcacggctg ttttccgcac ggaatgcggc aacctgcgca
    15721 tcgatgacgg aactgtcgat tttctgaccg ttgacggttg ccagcgtttg ggcaaacagg
    15781 ctgccggaac aggcaagcag tgcaacggaa gtcaggattt ttgctttcat aatcttctct
    15841 ctttaaattt taaattgaaa tgttcgggca gacttcattc agtccgccgt atggaaatac
    15901 tcgtcagggg tagccgcccg gatgccgagc gcgtgaatca tgccgcctga aaacaaatct
    15961 ttgagcagcg atttgaccgt tttctggcgg ttcaggcggc ttacgccttc aaaacggccg
    16021 ctaacgacca aaacggcata atgtccgccg ccggtattgc ccgcgtgtcc tttgtgcaga
    16081 tggctctcat cgccgatttc caacaccagc ggatcgagcg tctgcaggcg ttcgcggatc
    16141 aaatcgacgg ccggcattat ttgaacaccg ctttaaacgg cttgatcagc acttcgctgt
    16201 acacgcctgc atgaacatag ggatcgtctt ccgcccacgc ctctgccgca tccaaagact
    16261 cgaactgcgc cacaatcaaa ctgcccgaaa cgcggttgga gtcctccggc aaaggattcg
    16321 ggcctgccgt caacagccgg ccttccgact tcagcgtctc cagccgtttg aggtgttcgg
    16381 gacgtgccgc catacgcgcc tcgtgcacat cctccccgtc tgttgccagc aacataaaat
    16441 attccacagt caatcctcct ttttcagaca ggtactcaga taaataccct gaataataaa
    16501 gaaaacaagc atcagtgcag tcgagccgaa cattttatag ttgacccatt gcgactcgaa
    16561 ccgggtaaac acaaaccagt tggcgatacc cataaaaatc aggaaaccga cccacatata
    16621 cgtcaatttc gcccatacgg catccggaag ctgaatctcc ctgccgatac tcgccttcaa
    16681 gccgttttta ccggcgaggt ggctgcccca caggaacagc gcgcccagcc aaaacaaaac
    16741 gctcggcttc cacataatga agcggctgtc gcccaaaaca atggttgcgc cgccgaatac
    16801 cacaatcagc accaatccga cccactgcat cgtatccagc tttttatatt tccaatacag
    16861 aaaagccgcc tgaaccacac cggcaaccaa tgcgaccgcc gttgcggcaa tcatgttttt
    16921 ggtaacggta taggtggcga aaaacaggat gacggacaaa aggtcgctga cgaatttcat
    16981 atccaaatga gaaagcaaat caaataaacg ctaatcatac aggcagaacc ctgtttttgc
    17041 acctgcaaaa ccaacaaatt ttgttttgat tgatgcaaat atgcacagat gtttttgaaa
    17101 aaagatggag atatgtcata atttgtaaaa acggcgaatg attgccgttt aaaatgtggc
    17161 ggcggtcggt acattcacat attaaatgcc cgccggccga ctgccccgcc gatgcccgat
    17221 gatgacgaaa tttacagact gtacgcggtc aaaccgtatt cagccgccaa cccacagggg
    17281 atacatcttg aaaaacaaca gacaaatcaa actgattgcc gcctccgtcg cagttgccgc
    17341 atcctttcag gcacatgctg gactgggcgg actgaatatc cagtccaacc ttgacgaacc
    17401 cttttccggc agcattaccg taaccggcga agaagccaaa gccctgctag gcggcggcag
    17461 cgttaccgtt tccgaaaaag gcctgaccgc caaagtccac aagttgggcg acaaagccgt
    17521 cattgccgtt tcttccgaac aggcagtccg cgatcccgtc ctggtgttcc gcatcggcgc
    17581 aggcgcacag gtacgcgaat acaccgccat cctcgatcct gtcggctact cgcccaaaac
    17641 caaatctgca ctttcagacg gcaagacaca ccgcaaaacc gctccgacag cagagtccca
    17701 agaaaatcaa aacgccaaag ccctccgcaa aaccgataaa aaagacagcg cgaacgcagc
    17761 cgtcaaaccg gcatacaacg gcaaaaccca taccgtccgc aaaggcgaaa cggtcaaaca
    17821 gattgccgcc gccatccgcc cgaaacacct gacgctcgaa caggttgccg atgcgctgct
    17881 gaaggcaaac ccaaatgttt ccgcacacgg cagactgcgt gcgggcagcg tgcttcacat
    17941 tccgaatctg aacaggatca aagcggaaca acccaaaccg caaacggcga aacccaaagc
    18001 cgaaaccgca tccatgccgt ccgaaccgtc caaacaggca acggtagaga aaccggttga
    18061 aaaacctgaa gcaaaagttg ccgcgcccga agcaaaagcg gaaaaaccgg ccgttcgacc
    18121 cgaacctgta cccgctgcaa atactgccgc atcggaaacc gctgccgaat ccgcccccca
    18181 agaagccgcc gcttctgcca tcgacacgcc gaccgacgaa accggtaacg ccgtttccga
    18241 acctgtcgaa caggtttctg ccgaagaaga aaccgaaagc ggactgttcg gcggttcgta
    18301 caccttgctg cttgccggcg gaggcgcggc attgatcgcc ctgctgctgc ttttgcgcct
    18361 tgcccaatcc aaacgcgcgc gccgtaccga agaatccgtc cctgaggaag agcctggcct
    18421 tgacgacgcg gcagacgacg gcatagaaat cacctttgcc gaagtcgaaa ctccggcaac
    18481 gcccgaaccc gctccgaaaa acgatgtaaa cgacacactt gccttagatg gggaatctga
    18541 agaagagtta tcggcaaaac aaacgttcga tgtcgaaacc gatacgcctt ccaaccgcat
    18601 cgacttggat ttcgacagcc tggcagccgc gcaaaacggc attttatccg gcgcacttac
    18661 gcaggatgaa gaaacccaaa aacgcgcgga tgccgattgg aacgccatcg aatccacaga
    18721 cagcgtgtac gagcccgaga ccttcaaccc gtacaaccct gtcgaaatcg tcatcgacac
    18781 gcccgaaccg gaatctgtcg cccaaactgc cgaaaacaaa ccggaaaccg tcgataccga
    18841 tttctccgac aacctgccct caaacaacca tatcggcaca gaagaaacag cttccgcaaa
    18901 acctgcctca ccctccggac tggcaggctt cctgaaggct tcctcgcccg aaaccatctt
    18961 ggaaaaaaca gttgccgaag tccaaacacc ggaagagttg cacgatttcc tgaaagtgta
    19021 cgaaaccgat gccgtcgcgg aaactgcgcc tgaaacgccc gatttcaacg ccgccgcaga
    19081 cgatttgtcc gcattgcttc aacctgccga agcaccgtcc gttgaggaaa atataacgga
    19141 aaccgttgcc gaaacacccg acttcaacgc caccgcagac gatttgtccg cattacttca
    19201 accttctgaa gtacctgccg ttgaggaaaa tgcagcggaa accgttgccg atgatttgtc
    19261 cgcactgttg caacctgctg aagcaccggc cgttgaggaa aatgtaacgg aaaccgttgc
    19321 cgaaacaccc gatttcaacg ccaccgcaga cgatttgtcc gcattacttc aaccttctga
    19381 agcacctgcc gttgaggaaa atgcagcgga aaccgttgcc gatgatttgt ccgcactgtt
    19441 gcaacctgct gaagcaccgg ccgttgagga aaatgcagcg gaaatcactt tggaaacgcc
    19501 tgattccaac acctctgagg cagacgcttt gcccgacttc ctgaaagacg gcgaggagga
    19561 aacggtagat tggagcatct acctctcgga agaaaatatc ccaaataatg cagataccag
    19621 tttcccttcg gaatctgtag gttctgacgc gccttccgaa gcgaaatacg accttgccga
    19681 aatgtatctc gaaatcggcg accgcgatgc cgctgccgag acagtgcaga aattgctgga
    19741 agaagcggaa ggcgacgtac tcaaacgtgc ccaagcattg gcgcaggaat tgggtatttg
    19801 attcccaact gccctttcgc agatcaagga tgccgtttca gacggcatct ttttttgcct
    19861 tatcggtgta acggataaag tttgaaccgg cacaggctca aacagcaggc cgacggcaac
    19921 aaaatgccgt ctgaaacccc taaaggcttc agacggcatt ggcggcggcg gtatcaggca
    19981 gtttgatagg caaccgaatc gtcaccgctt ttcttttgaa tgaactcgat tttgtatccg
    20041 tcggggtctt cgacgaaggc tatcacggtt gtgccgtgtt tcatcgggcc ggcttcgcgg
    20101 acgacgtttc cgccctgccg cttcacacgt tcgcaggctt cgtaggcatc gtccacttca
    20161 accgcgatgt gtccgtaggc gttgcccaag tcgtatcgtt ccgtatccca gttgtgcgtc
    20221 agttccaaaa ccgtgctgtc ggtttcgtcg ccgtaaccga cgaaggcaag ggtaaatctg
    20281 ccttcgggat aatcttttcg gcggagcagt ttcataccca aaacgttttg gtagaaatcg
    20341 agggattttt cgagattgcc cacgcggagc atagtatgga gtaagcgcat ttttgtgttc
    20401 ctctcggtag tggttaaact tcgattttgt ttggcgtaaa cgtctgccat ttgttgcagg
    20461 caggacaatg ccagaaaaag acttgtgatt tgaagtggca gtttcggcac cggtacatca
    20521 cgctgcgctg tagctgccgt ccgataaccg aacgcatcat atcggcatcg gctttccaag
    20581 ccggatccaa atcgctgagt ttcaaaccaa gcaggcggta cacgccgttg aggtcgggct
    20641 tgcggcggac aagctcgacg gcggtttgcg cggcttcttt ctcgcactta agcagcaggg
    20701 atttctcgta cacgacattg atcaggtcaa gttcgggaaa cgtctgcata tatcctgtca
    20761 gacggttcaa gccttcttca ggttttccct gcgcggcata ggcttcgtaa agcttctcgc
    20821 cgaccatgct caagtatgca tggttttgct gctcgatggc ggcataggct tcgacggcgg
    20881 cagggaaatt gccttgtcgg tgttcgatgt cgcccaaaat catgttggca cgggtgcatt
    20941 ttttgttggc ttcgagtgcc ttgccgacat tgaaacgcgc gacatcgaaa ttggacttga
    21001 acagcgcggc ttgggcaagt tcgcaataaa actgggcgat ttcaaactga taggtctgat
    21061 cgtcatggct gagcagccgg gcggtttcaa ccgctttttc ccaatccctg tcctgttggt
    21121 agatattgag caggtgctgt ctggcttcac gcgccatttt accgtcttgc agccccaaaa
    21181 aaatctgttc ggcacgatcg accaaccccg cactttggta gttttgcgcc aattcaaaca
    21241 ggacgcgcgc gcgcttttcg ccgaccgtat cgggagaatc gagcattgtc cggtgtatgt
    21301 tgatggcttt gtcgttttcg ccacgctgac ggtaaagctt gcctagggtg aggtttaggt
    21361 cgtatgactg aggctgctga tcaatgacct ctgccaattc cctggcggca cgtccgctgt
    21421 tgcggtcaac caaggcatcc agacttttat aaaatcccga cggtatgctt tttgcctgct
    21481 ttaatacagt cttcatatcc acacgggcgg caaaccagcc catcgcgaag aaaacgggca
    21541 aaaggataat cggcagcagg ataatccaca attcattgtc catatcggct ttctcaaggc
    21601 tgtttggcag attcgggcgc attttgcgcc ggtggtgcgg tcagctcttt ccccgtcaaa
    21661 cgcgcatttt tctttacttc ggcacgcaac ctgccgttct cgccacgtaa cgacaacaac
    21721 cgtccgaaca aggcaaacat tccaaaaata ataccgacta caaatgcgcc gaacaatacg
    21781 acaatcagcg gcaaatcgaa tttttgcccc ggcaggtagg agaaggtaac ggcatccgta
    21841 ttaatgacgg caagcagcag gaagaacagc aggataatga ttttgacgac ggtatagata
    21901 agtttcatgt ttgctccgta aataaaaacg ccgcccggac agcttcgcac tctgaaccaa
    21961 gattgcgcta gtttaaacga tttgcacggt ttcggatagg atgcggcagc gtgttcggac
    22021 gacatacgga gtatgcgatt gccgacaatt ttaccaaata caccgttcct ttccatttga
    22081 aaaataacgg attggacacc gcatcgacag aaaaacccgc cgcgcacttg tcaaaaccct
    22141 gtttgcaggc gtatctttac aatcttcaaa ttcaaaccgt tcattgaaac atatcagaat
    22201 aagaaaggct ttacatcatg agcagacccg tacccgccgt attcggcagc gtttttcaca
    22261 gtcaaatgcc cgtcctcgcc taccgcgaag gcaaatggca gccgaccgaa tggcaatctt
    22321 cccaagacct ctccctcgca ccgggtgcgc acgccctgca ctacggcagc gaatgtttcg
    22381 agggactgaa agccttccgt caggcagacg gtaaaatcgt actgttccgt ccgactgcca
    22441 atatcgcgcg tatgcggcaa agtgcggaca ttttgcacct gccccgcccc gaaacccaag
    22501 cctatctcga tgctttggtc gaactggtca aacgtgccgc cgacgaaatt cccgatgcgc
    22561 ctgccgccct gtacctgcgc ccgaccttaa tcggtaccga tcccgttatc ggcaaggccg
    22621 gttctccttc cgaaaccgcc ctgctgtata ttttggcttc ccccgtcggc gactatttca
    22681 aagtcggctc gcccgtcaaa attttagtgg aaaccgaaca catccgctgc gccccgcata
    22741 tgggccgcgt caaatgcggc ggcaactacg cttccgccat gcactgggtg ctgaaggcga
    22801 aagccgaata cggcgcaaac caagtcctct tctgcccgaa cggcgacgtg caggaaaccg
    22861 gcgcgtccaa ctttatcctg attaacggcg atgaaatcat taccaaaccg ctgaccgacg
    22921 agtttttgca cggcgttacc cgcgattccg tgctgacggt tgccaaagat ttgggctata
    22981 ccgtcagcga acgcaatttc acggttgccg aactgaaagc tgcggtggaa aacggtgcgg
    23041 aggcgatttt gaccggtacg gccgcggtca tttctccggt aacttctttc gtgattgacg
    23101 gcaaagaaat cgaagtgaaa agccaagaac gcggctacgc catccgtaag gcgattaccg
    23161 acatccagta tggtttggcg gaagacaaat acggctggct ggttgaagtg tgctgatgct
    23221 ttaaataaaa aaatgccgtc tgaaacccgt ttggcgtttc agacggcatt ttcgcgttcg
    23281 gaccatttcc gccgcacctg cagcaagtcg gcataaaggc aatcggttaa aacaagcgtc
    23341 cgcatttccc atcccgcctg ccgcaagtcg ggcatttctc tggaaatacg cttcagacgg
    23401 caaaccgccg cccgaaaccg atatgcggca cggacggcgc acggatttga aaacggcgga
    23461 ttatccctcg gtgctcaagg cattaatgct gtaaccgccg tcaacgtaag tgatttcgcc
    23521 ggtaatgccg gacgacaggt cggacagcag gaaggcggcg gtattgccga cttcttcgat
    23581 ggtaacgttg cggcgcagcg gattgtgggc ggcgacgtgt cccaagagtt tgccaaaatc
    23641 ggcgatgccg gaggcggcga gtgttttgat cggaccggcg gaaatgccgt tgcagcggat
    23701 gccttctttg cccaaacagg cggcggtaaa gcggatgcct gcctcaaggc tagcttttgc
    23761 catacccatc acgttgtagt tcggaatcgc gcgcaccgcg cccaagtagc tcagggcgac
    23821 gatggcggag tttctgccgc gcatcatcgg acgggcggct tttgccagcg caggcaggct
    23881 gtatgcggaa atttcgtgtg cggtgttgaa cgcttcgcgg ctgatgctgt cgaggaaatc
    23941 gccgctcaag gcttctttcg gcgcgaagcc gatggaatgc accaaaccgt ccaagccgtc
    24001 ccaatgcttg cccaagtcgg cgaacacttg gttgatttcg tcgtcgctgg cgacatcgca
    24061 gcggaacacg agttcggaat ccaattccgc cgccattttg cggacgcgct cttccagttt
    24121 gtccacaacg taggtaaacg ccagttccgc gccttgttcg cggcaggctt tggcgatgcc
    24181 gtaagcgatg gaacgctcgg aaatcatgcc ggtaatcaga atttttttgc cttgcagaaa
    24241 acccattttg tatccttcaa atagtgttcg gttcgttcta aaccgttgat tatagcaaat
    24301 tgtccctgtt tctgtgtttt cacgttgcag catgcaaacg gcaatgccgt ccgaagcgga
    24361 tttcagacgg catcggacgt ttcaaatacg gtttaaggca tcagatgccg cgcaacaatt
    24421 cgttcacgct ggttttcgca cgggtttgcg cgtccacgcg tttgacgatg acggcgcagt
    24481 aaaggctgtg gctgccgtct ttggaaggca tactgccgga cacgacgacc gaacctgtcg
    24541 gtacgcggcc ttggtagatt tcgccggtgg tgcggtcgaa gattttggtg gattgaccga
    24601 tgaacacgcc catcgaaatc acgctgcctt cttcgacaat cacgccctca acgatttcag
    24661 aacgcgcacc gatgaagcag ttgtcttcaa tgatggtggg ggcggcctgc aggggttcga
    24721 gtacgccgcc gataccgacg ccgccactca agtgcacgtt tttaccgatt tgcgcgcaag
    24781 agccgacagt cgcccaagta tcaaccatcg cgccttcgtc gacgtatgcg ccgatgttga
    24841 cgtaagacgg catcagcacg acatttttcg ccacaaagct gccgcgtcgg gcaaccgcac
    24901 ccggaacggc gcggaagcct gcgtttttga actcgtcttc agaccagtcg gcaaacttgg
    24961 tcggcacttt gtcgaagtat ttgttcacgc cgtcgttgag gacttcgttg tcttggatgc
    25021 ggaaggacag caacacggct tttttcgccc attcgttgac tttccactca cccacgccca
    25081 agcgttcggc aacgcgcagt ttgcctgaat cgagttggcg gatggtttcc aacacggctt
    25141 ctttgacttc gggagtaacg gtggtcgggg taatgtccgc gcggttttca aaggcggttt
    25201 cgataatgtt ttgcaaagac ataatgtttc cttatgtgag atgtttcgtg ggacgcttgc
    25261 cggtattttc aagcagtatg ttcatttgaa atgaatgcgc tattgtgcca cgcaaacgca
    25321 tattcgtcag caatacacgg gcggttgccg aacattggcg tttcggaatc gggcgggcgg
    25381 caggtattgc cttttccgtg cccgaatcaa tacagaacgg cggcggcact tttatgccgc
    25441 gccgccgcct ttcagacggc attcgcggta aaacgccatc agcccttcgg tcgaggcatc
    25501 gtgtacggac gtgccgcctt ccagttcgcc gatgatggtt ttcgccaact gtttgccgta
    25561 ctcaaccccc cactgatcga aggggttgac gttccatatc gcgccttgga cgaaggtttt
    25621 gtgttcgtaa gccgccatca gcatacccag attgtagggc gtgaggcggt cgagcaggat
    25681 gctgttgctg gggcggttgc cggggaattc tttgtgcggc gcgaggcgtt cgcgttccgc
    25741 ttcgggcaaa tctgccagtt cggcgcgtgc ttcgtccaag gttttgccct tcatcagggc
    25801 ttccgcttgg gcaaaggcgt tggcaacggt aaaacggctg cgtccgtcct ccacgccctg
    25861 cgccgtcatc gggacgataa agtcgcaggg aatcaggcgc gtgccttgat gaagcagttg
    25921 gaaataggcg tgctggcagt tgaccccttc accgccgaac acgatgccgc ccgttttgca
    25981 cacggcggga ctgccgtctg aagcgcggct tttgcccaaa ctctccatat cgagctggtt
    26041 cagccacgcc ggcagcaggc gcaggttgtg gctgtacgga acggcagtct gcccatccgc
    26101 gtgctggaaa ttgttatacc acacggcaat cagtgccatc agcacgggaa tgttgcggcg
    26161 cagcggcgtg tgaaaaaaat ggctgtccat cgcgtgcgcc cccgccaaca actcgcggaa
    26221 acgcgccccg ccgaccgcaa ccatcacggg caaaccgacg ggcgaccaga cggaatagcg
    26281 tccgcccacc cagtcgtaca tcgcaaacac gcgttccgcc gcaatgccga aactttgcgc
    26341 cgcttccgta tcggcagaca ccgcgcaaaa atggcgcgcc gtttcggatt ccgagaaccc
    26401 cgcaccgcga taccacgcct tgaccgcctg tgcattgagc agggtttctg gtgttttgaa
    26461 ggacttgctg gcaacgcaaa acactgtcgt ttcggggttc agacggcata aaaccgcatc
    26521 caggcaggca ggatcggcgt tggcggcaaa atggacggcg atatgccgtc tgaacggctc
    26581 gagtgcctgc acgcacattg ccggcccgag gtcggatccg cctatgccga tgtggacaaa
    26641 atccgtaatc cgtttcccag ttatcccctg atacgaaccg tcgtccaaac tgtgtgcaaa
    26701 ctccaacgca cgatttaact cgcggcggat ttcgggcaac acgtccctgc cgtccgcata
    26761 aacggcatcc gcaccgtcgg gtaggcgcaa agccgtatgc agcgcggcac gcccctcgct
    26821 gccgttgact ttcgcacccg tccgcaaagc acgcattttc ccttccaaat ccgccgtctc
    26881 cgcaagacgg cagagcagtt gcagcgtatc ttcgcccaaa cggtttttgc tgtaatcgaa
    26941 caacatcccg tccaaacgct catgcatacg ctcaaaacgg tcgggttcgc aggcaaagcg
    27001 gtcgcgcaaa aggacatgac gcgtatcctg ataatggcgt tcgagcgcat accatgcacg
    27061 ggtaaaagca ttcatctgtt ttccttgatt tttcagaacc ggattaaaat gtagcagaat
    27121 gtagtttaac aaacggcagc ggctttggcg aatctccgga acaccgcccg ccgcgtccaa
    27181 tagcacctta tttccatatg gcgaaaatat agtgaattaa caaaaatcag gacaaggcgg
    27241 cgagccgcaa acagtacaaa tagtacggaa ccgattcact tggtgcttca gcaccttaga
    27301 gaatcgttct ctcttgagct aaggcgagcc aacgctgtac cggtttttgt taattcacta
    27361 taccctccgg ccggcatttg acccgcaaca atatcctgca agatttattg tgtacgcata
    27421 aatgccggac agccgcctaa ataaggtata atcgcatccg attctgtccc gtttgccgcc
    27481 cgcaggcggg atgtcggcgg ttttcatctt accgggaaca cgccggtact gttttttaac
    27541 gatgtttctt acattttatt ccaattactt tacggggcta gaatatggct aaaaacggag
    27601 gattttcttt gttcgcaaag aaagaaaaac gctttatctt tgaaggcagg cattccgcct
    27661 ccgacaaact ggtcaacggc gaagtatccg cgtttaccga agaagaggcg cgcaaaaaac
    27721 tggcaaaacg cggcatccgc ccgttgcaga ttacccgtgt gaaaacaagc tccaagcgca
    27781 aaatcacaca agaagacatc accgttttca cccgccagct gtccacgatg attaaagcgg
    27841 gcctgccgct gatgcaggca tttgaaatcg tggcgcgcgg acacggcaac ccgtctatga
    27901 cggaaatgct gatggaaatc cgaggcgaag tggaacaggg cagctcgttg agccgcgcat
    27961 tctcaaacca cccaaaatat ttcgaccgct tctactgcaa tctggttgcg gcgggcgaaa
    28021 cgggcggcgt attggaaagc ctgctggaca aattggcaat ttacaaagaa aaaacccagg
    28081 ccatccgcaa aaaggtaaaa accgcactga cctatccggt atccgtcatc gccgtcgcca
    28141 tcggtttggt attcgtgatg atgattttcg tactgcccgc ctttaaagaa gtttacgcca
    28201 atatgggcgc ggagcttccc gcactgaccc aaacagtgat ggatatgtcc gactttttcg
    28261 tctcatacgg ctggatggtg ctgatcgcac tgggctttgc catatacggc ttccttaaat
    28321 tgaaggcgcg ttcgattaaa atccaacggc gtatggatgc catactgctg cgtatgccga
    28381 ttttcggaga cattgtccgc aaaggaacga ttgcccgctg gggcaggacg acggcgacgc
    28441 tgattgcggc aggcgtgcct ttggtcgatg tattggactc cactgccggc gcggcgggca
    28501 atttaatcta tgaagaagcc acccgggaaa tccgtacgcg cgtcatccaa ggtttgtcta
    28561 tgacttcggg gatgcgtgcg acggaactgt tccccaatat gatgttgcag atgtcttcca
    28621 tcggcgagga atcgggttct ttggacgata tgctcaacaa agccgccgaa ttttacgaag
    28681 acgaggtgga caatgcggtc ggcaggctgt ccgctatgat ggagccgatc attatcgtga
    28741 ttttaggctt ggtcatcggc acgcttctgg tcgccatgta tctgccgctg tttaatttgg
    28801 gcaacgtggt cgcctgattt gccgcacaga tccggcgcgg attcgctctg cgccggtttg
    28861 tttttgtttt gaatacattg agaacaaaat atgtctgatt tgtctgtatt gtcgccgttt
    28921 gccgtgcctt tggcagcggt gttcgggctg ctggtcggaa gtttcttaaa tgtcgtcatt
    28981 taccgcgtgc cggtcatgat ggaacgcggc tggacggtat ttgccaaaga atatttaaac
    29041 ctgccgctga ccgaagagga aagccgtacc ttcaacctga tgaaaccgga ttcctgctgt
    29101 cccaaatgcc gcgtgccgat acgcgcgtgg cagaacatcc cgattgtcag ctacctgctc
    29161 ctgcgcggca aatgcgcttc ctgccaaacc aaaatcagca tacgttatcc cttaatcgag
    29221 ctgctgaccg gcgtattgtt cgggctggtc gcctggcaat acggctggtc ttggattacg
    29281 ctgggcggat tggtactgac cgcgtttctg atttccctga cctttatcga tgcggacacc
    29341 caatacctgc ccgactcgat gacactgccc ttaatttggc tgggtctgat atttaatttg
    29401 gacggcggct tcgtgccttt gcagtctgcc gttttaggtg cggtcgccgg ctatggttca
    29461 ttatggctct tatgtgcagt gtataaactg ctcacaggaa aaaccggtat gggcaacgga
    29521 gatttcaaac tgattgccgc attgggcgcg tggctcggca tatccgcatt gcccgtactg
    29581 atttttgttt cctcgctgat cggtttggtc gcggcaatcg ttatgcgcgt cgccaagggg
    29641 cagcattttg ccttcggccc cgcactgaca gtttcgggct ggataatttt tacggcaaac
    29701 gattccgtat ggcgggcggt caactggtgg ctgacccatc cggtgctgta agatgacggt
    29761 atgggtcgga ctgaccggcg gaatcggcag cggcaaatcg gcagccgcgc aatgttttgc
    29821 cgatttgggc gtgccgcgca tcgatgcaga cgcggcggcg cactcgctga cggcttcaga
    29881 cggcatcgcc ctgccggaaa tcaggcggct gttcggcgac accgtttttg acacacaggg
    29941 tttgttgcgg cgcgacatat tgcgtaaaga agtctttgcc tccccatcgc gaaaagcctt
    30001 gctcgaatcc gtgatgttgc cgctgatttt ctcagaaatc aaaaaacagc aagaaacctt
    30061 tactgatgca gcttacggca ttgtcgaaat tccgctgctg acggaaaagc gtcaatttat
    30121 cagcctgata cggcgtgtcc tgaccataag cgcacctttg gaaaaacgta tcggcagggt
    30181 gatggcccgc agcgggctga cgcgcggcga ggtggctgcc gtcatcagcc atcaggcatc
    30241 cgaatccgaa cgcctgctgc ttgcagacga tgtactgctc aatgacggca gcctcaaaag
    30301 cctacgtgag aaaacaatgc gcctgcacgc gttttattca gggattttcg cctcaaaacc
    30361 aacacaagga aaacacaatg gctgaatcgc ggcaaacacg ccttcaagtc aaatgtccga
    30421 cctgtcaaac ggcagtagta tggaaaccgg aaaacgcatt ccgccccttc tgttcgcaac
    30481 gctgcaaact gatcgacttg ggcggatggg cagatgagga atatacggtt acagcccaag
    30541 aagaaggttt attggaaata tccgaacccg acggcgcata ccgctgaccg caccccgtcc
    30601 tccgacaaac ccctgaaaat caaatgccgt ctgaaacaaa cacgcttcag acggcatttt
    30661 cattctcaaa cctaatcgtt ggtatttgcc gttacctctt ccaatgaagt aatgccctgc
    30721 ataactttca aaataccggc ccggcgcaaa tccaccatac cctccttata ggcaacgtcc
    30781 aaaatatcca cttccgtacc gttgttcata atcacacgct gcatttcttc gctgatgggc
    30841 ataacctcat acacgcccgc acgcccctta taaccctgtc cccggcaacg gtcgcaaccg
    30901 acggcgcggt aaagtttcca atcttttgca aggtcctcat cggtgaagcc gacttccttc
    30961 aaagcagagg cagacgggcg ttccacttcc tgtttgcagc tcgaacacag cctgcgtaaa
    31021 agacgctgcg ccataatcag gctgaccgaa ctggcaatat taaacggcgc gacacccata
    31081 ttcagcatac gcgacaacgt cgccggcgca ttattcgtgt gcagtgtgga aaacaccata
    31141 tgccctgttt gtgccgcctt aatcgcaata tcggcagttt ccaaatcacg aatctcaccg
    31201 accataatga tgtccgggtc ctgacgcagg aaagacttca aagcagcggc aaaagtcagg
    31261 ccctgcttat cattgacgtt aacctgattg atgcccggca ggttaatctc ggcagggtct
    31321 tccgccgttg caatatttac cgactccgta ttcaaaatat tcaaacaggt atagagcgac
    31381 accgtcttac ccgaacccgt cggaccggtt accagcacca tcccgtaggg acggtgaatc
    31441 gcttccaaca acaatttttt ctgaaacggc tcaaaaccga gctggtcgat gttcaaagac
    31501 gcggcatcgg aattcaaaat ccgcatcacg accttttcgc caaacagcgt cggcaatgtg
    31561 ctgacacgga aatcgacagg cttgccgccc ttttgaaagg tcagctgcat cctgccgtcc
    31621 tgcggtatcc gtttttcgga aatgtccaaa cgcgacatta ccttaatccg ggaagcaagc
    31681 tgccccctta ccgcaatggg cggctgaacc acctcgcgga gctgcccgtc cacacggaaa
    31741 cggatacggg cattgtgttc gtaaaactcg aaatggatgt ccgatgcccc gctgcgcaag
    31801 gcatccgaca aagtcttatg gataaacctc ggaacagggc cgtcttctgc ctcctcgttg
    31861 tcgatataca gggtgtggct ttcctcttcc tcctgcccct ccccaagctc ctgaagcagc
    31921 gatgtcgaac gcgaacccac ccaatcgagc aaacccgcca actggtcatc ctcgacaatg
    31981 accaactcaa cctcaatccc tgcggcagaa acggttttct gaatttgcgg catctgtgtc
    32041 ggatcggaaa ccgcaaaaaa tactttgtcg ccccgacgga aaaccggcac acagtggaac
    32101 tccaccatct gctcctccgt caacaccccc atcagcaccc tgtggcgcgg ataatgacgc
    32161 aaatcaagaa tcgaataact gaacaccctc gcaatcaatg ccgcaagcga cttgggcgaa
    32221 atgacaccgt ctgaaaacag catcggcaac acttccttac ccgcctgcga ctcattgtag
    32281 taatgctcgg cctgctcaac agtaaccacc tggttttgaa ccagaatcct cagcaaacct
    32341 acgctcatac gaccttatcc ccaaatttat tcattgttat acctgtacag cttttatagt
    32401 ggattaaatt taaaccagta cggcgttgcc tcgccttagc tcaaagagaa cgattctcta
    32461 aggtgctcaa gcaccaagtg aatcggttcc gtactatctg tactgtctgc ggcttcgtcg
    32521 ccttgtcctg atttaaattt aatccactat atcataaaac aacagatttc caagccggaa
    32581 catcttttac ggagcctgaa aatcatttca tgattctacc gtcctaaagg tcggtttttc
    32641 aagcaggaag aaaaagtttt cagatggcaa aaaagccctc caacactgaa aggccttata
    32701 tcggaaactt cccgcaacac gggaaacaga caaatgcaaa tcgtcaaacc tcgccaacag
    32761 gaatcgaacc tgtattttac gcttaggagg catacgttct atccgttgaa ctatggcgag
    32821 ccgaaatgaa aaggagattt taaccctttc cgacgacaaa agacaagatg ccggccgcaa
    32881 tcagcggccc gacagggata ccgcccataa atgccacccc gataaccgtc ccgattaaca
    32941 gccctgtaat taaaacaggc tgctgtccca tcaaaggcac gccgcgtccc gccagccaag
    33001 ccacgaaaat cccgataaaa acggcggata tcattttaaa attcaaaaat tcggcaacgg
    33061 gaggaacctg cgcctttcct gaaaccaacg gactcaaaac ccctatggtc aaaagaatga
    33121 taccgagatt caacccgtgc ttctcgacca acgggacaaa ctgtatcaat gccgtctgct
    33181 gcatcagcag caatatggtt gccgagatgg taatcgaatt gttgttgctg accaccccca
    33241 acagaatcag cgtaaccaga aacaggggag caaaactgac attcatcgcc tcaagacacc
    33301 ctgaccaaag attccttcgc cagctgaatc atggcatcct tcacttcgct gtcgggcagg
    33361 gcatccaagg cggtaacggc acaatcgact gctttgcgcg cctcgcctat cgaatatgcc
    33421 aacgcatccg aacggacgac ataatcgtgg attttctcaa aatagctgcg atctgcattt
    33481 tccaaagcag tacgcacatc gttcgcaacc tgttcggaac cctgacgcat cagataaatc
    33541 aaaggcaaag tcggttttcc ttccgccaaa tcgtcgccga cgtttttgcc ggtttcttcg
    33601 gtttcgccag aatagtccag cacatcgtca ataatttgga atgccgtacc gacatacata
    33661 ccgtagtctt tcaacgcccg ttcgtgttcg ggggaagcct tgcccaaaat tgcgccgact
    33721 tgagcggcag cttcaaacaa ttttgccgtt ttatattgga tgacttggat atattgttct
    33781 tcggtaatgt ccgtattgcc gatgttcatc agctgcatga cttcgccctc ggcaataatg
    33841 ttggttgcat ccgccatcac ttccaaaacg cgcatactgc ccgaggcaac catcagttga
    33901 aaggcgcgcg tgtataaaaa gtcgccaacc aacacagccg ccgcattgcc gaacagattg
    33961 tttgccgttg cccgcccacg gcgcaaatcg ctttcatcga cgacatcgtc gtgcaggagg
    34021 gtggaagtgt ggataaactc gaccatcgcc gccagcgaat acagtttctc gtcatcataa
    34081 ccgaccgcct tacccgccaa aatcgtcata atcggacgca ggcgtttgcc gcccgcgctg
    34141 atgatatatg taccgatttg cgaaatcagt gcgacatcgg attgcaccgc acggttgatg
    34201 acttcattga ctttggcaag gtcttcaggc agatgtcgct ggaaataggg cagattctcg
    34261 agcatggcag actctcaggt ttacgtttcc gtcaaacggc agataccgct tgaaagctga
    34321 ttcaaatagc gggcaattat atcagcaaag ccatcagggt acatcttttc agacggcata
    34381 tggttatccg aaccgcgcaa actttgacaa aacaggcaaa taacaataca atgccgagtt
    34441 ccgcacaatg gtgtgcggat attaaccata acccttatgg agttgagtat gtacgcggtc
    34501 gtaaaaaccg gcggcaaaca gtataaagtt tccgtcggcg aaaaattgaa agtagaacag
    34561 ataccagccg aactcgacag ccaaatcgaa ctgaccgaag ttttgatgat tgctgacggc
    34621 gaatctgtaa aagttggcgc accctttatc gaaggtgcaa aagtaacggc taaagtagtg
    34681 gcacacggtc gtggcgaaaa agtccgcatc ttcaaaatgc gccgccgcaa acactaccaa
    34741 aaacgccaag gccaccgcca aaatttcacc caaatcgaaa tcgtggcaat cgcctaacct
    34801 taaaacttag gagcattaca aatggcaagt aaaaaagcag gcggcagcac ccgcaacggt
    34861 cgcgattcag aagccaaacg cttgggcgtt aaagcctacg gcaacgagct gattcccgca
    34921 ggttccatca tcgtacgcca acgcggtacc aaattccacg caggcgacaa cgtaggtatg
    34981 ggcaaagacc acactttgtt cgccaaagtt gacggttatg tcgaattcaa aaccaaaggc
    35041 gcgctgaacc gtaaaactgt cagcatccgt ccttacactg gttctgaaga ataatccaac
    35101 cgatacctga agccgcatct tttcacgatg cggcttttta tagtggatta acaaaaatca
    35161 ggacaaggcg acgaagccgc agacagtaca aatagtacgg aaccgattca cttggtgctt
    35221 cagcacctta gagaatcgtt ctctttgagc taaggcgagg caacgccgta ctggtttaaa
    35281 tttaatccac tatacatacc ccacacccga tttgatacac attaagctga aagtaaaaat
    35341 ccgcatacac cctcccctgc atatttcttc aacagtgggt tttgatataa tcgcctatct
    35401 gttacagata gttcaaacgg cattcaaacc cttacaaata ccgtctgaaa tcatcggcat
    35461 ccgtctatgc aaaggatatt ttatgagttt acacagcgac atcctcgtcg tcggcgcagg
    35521 cccggcgggt ttaagttttg cagccgaact tgccggcagc ggtttgaaag ttaccctgat
    35581 tgaacgcagc ccactgactg ttctgcaaaa tcctccatat gacggacgcg aaatcgccct
    35641 gacccatttt tcccgcgaaa tcatgcagcg tttgggtatg tgggacaaaa ttcccgaaaa
    35701 cgaaatctac cctttgcgcg atgccaaagt gctgaacgga cgttccgact accagctcca
    35761 ctttccccaa ccgaccgagg cgcgcggcga acctgccgac tgtttgggct atcttatttc
    35821 caaccacaat atccgacgcg ctgcctacga agtcgtatcc caactcgaca acattaccat
    35881 tctgaccgat acggttgtca aagaagtcaa aacatctgac aacgaagcac aagtcatttt
    35941 ggaaaacggc aaaatcctga ctgcacgcct gcttttggcg gcagacagcc gcttctcgca
    36001 aacccgtcga caactcggta tctcctccga tatgcacgat tacagccgaa ccatgttcgt
    36061 ctgccgtatg aaacataccc tttccaacca acataccgct tacgaatgct tccattacgg
    36121 acgcaccatc gcactgctgc cgctggaaga gcacctgacc aataccgtca ttaccgtcga
    36181 taccgataaa atcaacagcg ttcaaaacct ttcgcccgaa gaattggcgg caagcgtaaa
    36241 agaacagctc aagggcaggc ttggcgacat ggaactggtc agcagtatcc accattaccc
    36301 ccttgtcggt atgattgcca aacgtttcta cggtaaacgc agcgcgctga tcggcgatgc
    36361 ggctgtcggt atgcaccccg ttaccgcaca cggtttcaac ttgggtctgt ccagcgcaga
    36421 tattttggca aaactgatac tcgaagccga gcagcgcggt caggacatcg gcgcggcttc
    36481 cttgttggaa aaatacagca gcaaacatat gctgcatgcc cagccgcttt atcacggaac
    36541 caatatgatg ctgaaactgt ttaccaacga aaccgcgccg gcaaaactgc tgcgcggttt
    36601 ggtattgcgt gccggcaaca acttcccgcc gctgaaaaag ctgattacca aacaactgac
    36661 cggttgaaaa ctgccgaaat gccgtctgaa acgtttcaga cggcattttt gtattatagt
    36721 ggattaacaa aaatcaggac aaggcgacga agctgcagac agtacaaata gtacggaacc
    36781 gattcacttg gtgcttcagc accttagaga atcgttctct atccttggca gacggtttgc
    36841 agcagattcg catacagcag atgtttcaag cggaaaagaa acacgataaa aataaaaaag
    36901 cctccgaaca gtcggaggct ttgctttcaa actgaaatta tttcagtttg gtttctttgt
    36961 acactacgtg tttgcgggca accggatcga attttttgat ttccagtttg ccgggcatag
    37021 tgcgtttgtt tttggtagtg gtgtagaagt gaccagtacc tgcactggat tccagtttga
    37081 ttttatcgcg cattgcagta atccttaata agggtttaaa ttaagcttca ccgcgagcac
    37141 gcaaatcagc caatacgaca tcaatgccta ctttgtcgat ggtacgcaga gcggcgttag
    37201 aaacgcgcag gcgaacccag cggttttcac tttctaccca aaaacgacgt gattgcaagt
    37261 tgggcaaaaa acggcgtttg gttttgttgt tggcgtgcga tacgttgttg ccggacatcg
    37321 ggcgtttgcc ggtcactttg caaactcgtg ccataatcag tctccaaact tctaaaatag
    37381 taaaacgcga tttataccac gaaaaacagt gttagttcaa gtgttttaaa gaaaaacggc
    37441 tgttttgcgc ggataatcct tccgattgtc gcatctgtgg aacgtttgca ggtgcgaaac
    37501 agatggggcg cgattatagc ggatttcggg aactgctgca ttcaaaatgc cgtccgaaca
    37561 tatttccagt attttcagac ggcatttcaa gttttcaaat ccctcaatgt ccaccgccgc
    37621 cgccgttgtg gaacggcggt tttgccagcc atatgacggg tatcatgata atgaataaca
    37681 agctgcccgc cataaagatt tcgttcgagc cgataataaa accctgctgg gtaatggtat
    37741 tgttgatgat gcctagggtt tgaatgtcgg aaacgccgtg ctgggacaaa tgagcggccg
    37801 tttcgtgcaa tgttgcggaa tagggcgtga tgtgttcggc aaagcgtgta tggtgcaacg
    37861 cttcacgccg ttcccacagg gtactaacga cggatacgcc gacaccgccc atcagcacgc
    37921 gcaggaaatt cgacaggctg ccggcggcgg caatctgtcc gcccttcatg tgcgacagtg
    37981 tgatggtggt cagcggcagg aagaacatgg caacgcctac tccctgccaa aactgcggcc
    38041 agatgacgtt gccgatatcc atgtcggcat aaaaatcggt gcgccaatag aaagtgaagg
    38101 caaaagtcag gaagctggcg gttacgagca ggcgcatatc gattttattg ccgaacctgc
    38161 cgattaacgg agacaggaag acgggcagga tgccgacggg tgcggcggca agccccgccc
    38221 acgtggaggt atagcccagg ttggtctgca acactaacgg cagcagggtc agcgtcccca
    38281 tatacaccat aaaacccaat gatgtggcaa tgatgccgac ggtaaaattc cgatctttaa
    38341 acagcgataa atcgacaatc ggatattttt ctcccaattc ccaaacaata aaatacgaca
    38401 agcacaccag tgcggttacg cccaaggtaa tgatttctcc cgaagcgaac cagtcgagtt
    38461 ccttacccct gtccagcatc atctgtaacg cgccgatacc gactaccatc aatgtaagcc
    38521 cgacatagtc ggtcggcatt ttaacggttt ccgtttcccg atatttcaaa tgtttccatg
    38581 taatccatgc cgatatgata ccgataggga tattaatgaa gaaaatccaa ccccaatgcc
    38641 agtttccgga aatccagccg ccgaatatcg gcccgagaac aggggcgaca acgacggtca
    38701 ttgcccacaa tgccagtgcc agcgtccgtt ttgcgggcgg ataggatgcc attaacaggc
    38761 tttgcgacag gggaatcagc ggcccggcga taaaaccctg caagatgcgg aaaaacacca
    38821 aagcctgaag gttgggcgcg atgccgcaca gccatgatgt gatgacgaaa ccgacagcgg
    38881 cggcggtaaa cagtttgacc tcgccgatac gttttgccaa aaagcccgtt aacggcacgg
    38941 aaacggcgtt tgccacagca aaagaagtaa tgacccacgt cccctgcgtg gttgccgcac
    39001 cgaggttgcc ggcgatgacg ggaacggcga cattggcgat agtcgtatct aaaacttcca
    39061 taaatacggc aagccccaaa gacagcgtaa cccacgccaa tgccgcaccc ttaagcggtg
    39121 gataatccat tgtgtcctcc gtttcagacg gcatcagggc gcggattgcc cgaggatttc
    39181 atcgaccgtc cgatcgactt ccgaccagtc ggtactttcc atttccggca atgccgcacc
    39241 cggcgttttt gaaacaggcg cgcctgcggc ggaagtatcc actttaaccg tcatcgacaa
    39301 accgatacgc aacggatgcc tgtccacatc ttcgcggttc agcacgatac ggacggggac
    39361 gcgctgcacc actttaatcc agttgcccgt tgcgttttgc gccggaatca gcgaaaacgc
    39421 gctgcccgta cctgccgaaa accctgccac cctgccgcga taaacaattt gtttgccgta
    39481 caaatcggac accagttcgg caggctgtcc gattttcata tgccgcaact gcgtctcttt
    39541 aaaattggca tccacccaca catccgacag cggcaccacc gccatcagcg gcgcgcctgc
    39601 cgccacctgc tgcccgacct gcaccgaacg cttcgccacc tgaccgtccg ccggcgcgcg
    39661 gatttgcgtc cgctgaaggt tcaaccacgc atctttcaac ctgccgattg ccgtctgaac
    39721 cgccggctgt tcgcgcaaag aaacctgacc gcccaaagcc gcgcgtgccg aagattcttc
    39781 cgccaaagcc gctttgaccg ccgcctgagc ctgagacact gccgcacggg catgtgccag
    39841 ctcttcggcg gacaccgcgc ccgattccgc caaagcagac cggcggcgca aatcatcctg
    39901 cgcacgtgcc aaatccgccc ggcgcaaggc aacctgcgcc cccgcctgcg aagtggcggc
    39961 attttgccgg cggttttgcc gcaccgcctg aatcagctcg ttttttgccc gctcgtaagc
    40021 cagcacatca ttatcgtcgt ccaataccgc cagcacgtcg ccttttttca cggcatccgt
    40081 atcgtcgtgc aaaaccttcc gcaccgtacc gcccttttgc ggcgtaacct gaaccacgcg
    40141 tccggcaaca taagcgtctt ccgtttcctc ttcgtgctgc caccataaaa aaaacgccga
    40201 cccggcggct gcggcggcaa gcgcgaacag cagcgtcaat gccgtcaggc agcgtttgcg
    40261 tttggcttgc gtgttttgaa gttttgtttc gtccgtgtgc gtatccataa gattgggttc
    40321 cgaatttcct gatttcatat aaacaatgtt ctttcaatag gaggcagatt ctatatccgc
    40381 gcaataacgc aatcaagtca gtattttaga tgcaatattc aaaaaacaac gcttcaggcg
    40441 gcaaaactga aaatatattt cgtttatata gtggattaac aaaaatcagg acaaggcggc
    40501 gagccgcaga cagtacggat agtacggaac cgattcactt agtgcttcag caccttagag
    40561 aatcgttctc tttgagctaa ggcgaggcaa cgccgtactg gtttttgtta atccactata
    40621 tatgacacgg aaaacgccgc cgtccaaacc atgccgtctg aagaaaacta cacagatacc
    40681 gccgcctgta ttacaatcgc cgccccgtgg ttcgaaaacc tcccacatta aaaaactaag
    40741 gaaaccccct atgtcccgca ataccgaaga gctgcaaggc atctcgcttt tgggtaatca
    40801 gaaaacccaa tatccgatcg gctacgcgcc tgaaattctc gaagcattcg acaacaaaca
    40861 tcccgacaac gactatttcg tcaaattcgt ctgcccagag ttcaccagcc tctgccccat
    40921 gaccgggcag cccgacttcg ccaccatcgt catccgctac attccgcaca tcaaaatggt
    40981 ggaaagcaaa tccctgaaac tctacctctt cagcttccgc aaccacggcg attttcatga
    41041 agactgtgtc aacatcatca tgaaagacct cattgccctg atggatccga aatacatcga
    41101 agtattcggc gagttcacac cgcgcggcgg catcgccatt catccgtttg ccaactacgg
    41161 caaagcaggc accgagtttg aagcattggc gcgtaaacgc ctgtttgagc acgacgcaca
    41221 ataaaccatc cttcccactt tcagacggca ttccttcgat acgatatgcc gtctgaacgc
    41281 ctttccgaaa gatttttatg tacgcattta ccgacgcaca gcaacagaag gcactcttct
    41341 ggctggtgct tttccatatc ctcatcatcg ccgccagcaa ctatctggtg cagttcccct
    41401 tccaaatttt cggcatccac accacttggg gcgcgttttc ctttcccttc atcttcctcg
    41461 ccaccgacct gaccgtccgc attttcggtt cgcacttggc acggcggatt atcttttggg
    41521 tcatgttccc cgcccttttg ctttcctacg tcttttccgt tttgttccac gacggtggtt
    41581 ggacaggctt gggcgcgctg tccgaattca acacctttgt cggacgcatc gcgctggcaa
    41641 gttttgccgc ctacgcgctc ggacaaatcc tcgatatttt cgtattcgac aaattacgcc
    41701 gtctgaaagc gtggtgggtt gccccgactg catcaaccgt catcggcaac gccttagata
    41761 cgttggtatt tttcgccgtt gccttctacg caagcagcga tggatttatg gcggcaaact
    41821 ggcagggcat cgcttttgtc gattacctgt tcaaacttac cgtctgcggt ctgtttttcc
    41881 tgcccgccta cggcgtgatt ctgaatctgc tgacgaaaaa actgacgacc ctgcaaacca
    41941 aacaggcgca agaccgcccc gcgccctcgc tgcaaaatcc gtaacacagc cctgccggga
    42001 atcgggacgg ttgggttttc ttttatttgc aaacactgtc ggaacggata aatccggatt
    42061 cccacccact tcgtcattcc cgcgaaagcg ggaatccggt acgttgggtt tcagtcattt
    42121 ccgataaact cctgccgcgt tgtatttcta gattcccgct ttcgcgggaa tgacggcgga
    42181 tgggtttccg tgccgtggtg tccggattcc cgcccgtacg ggaatgacgg cagtgcttcc
    42241 ttggcttagt tttattttaa tcgactgtat
//
LOCUS       DAWFHY010000005        42040 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.005, whole genome shotgun sequence.
ACCESSION   DAWFHY010000005 DAWFHY010000000
VERSION     DAWFHY010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 42040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42040
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.005"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(175..867)
                     /locus_tag="WGQ68_000211"
     CDS             complement(175..867)
                     /locus_tag="WGQ68_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0714095.1"
                     /translation="MKRFTYTLSDGLCIEIELKRSAKKNLILRPVNMQTVSINVPPFF
                     QDHALANWLAANETILRNTLAKTPVHPVSHPNLPEWIWYRGIKTKLDTHSQSHIRITS
                     SEILLPRKETAAQIDHLRRLLNERAREYLLPRLEKHAAETGLTPTATDLSNAKTFWGV
                     CRPHTGIRLNWRLIGAPKYVADYVCIHELCHLRHPDHSPRFWHLVNTLTPHTDNAKSW
                     LKAHGRELFVLG"
     gene            complement(975..2168)
                     /locus_tag="WGQ68_000212"
     CDS             complement(975..2168)
                     /locus_tag="WGQ68_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0714096.1"
                     /translation="MSNQKNRRNALIGAAFLMATSAIGPGFLTQTATFTQALAASFGF
                     VILLSILLDIGAQLNIWRIVAVSEKQAQDIANQVLPGAGYFLAVLIVMGGLAFNIGNV
                     GGAGLGLNLLTGLSPETGAVISGVIAIGVFLFKEAGKVMDKFAQVMGFVMIALTVYVA
                     WQANPPLADAAVHTFMPEKLDAMAIVTLVGGTVGGYITFAGAHRLLDAGIKGKSALPE
                     VSQSSVRAILIASIMRIVLFLAVLGVVSQGVQLNPDNPASTPFEYAAGYIGLLIFGVV
                     IWAASITSVIGAAYTSVSFFSGLSPSIERNKNKWIIAFIAVSTAVFSTIGKPAQVLVF
                     VGALNGLILPISLGLILIAAYKTKIVGDYKHPLWLTVSGVIVVGLMAVLSAITISKYI
                     GGLFG"
     gene            complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WGQ68_000213"
     CDS             complement(2297..3034)
                     /gene="pxpA"
                     /locus_tag="WGQ68_000213"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HEZ0714097.1"
                     /translation="MKQVDLNADLAEGCGSDEALLQLITSANIACAQHAGSIADIRAA
                     LAYAQQNGVRIGAHPGYPDRENFGRTEMNLSEADLRACLNYQLGALQALCRDQGLEMA
                     YVKPHGAMYNQAAKNRALADTVARIVADFDPKLKLMALSGSLLLEAGKAAGLGVISEV
                     FADRRYMPDGTLVPRSRPDAQVDSDEEAIAQVLQMVRDGRVKAVNGSLVAVQADSICL
                     HGDGPHAVVFAEKIRQELLAAGIKVSA"
     gene            complement(3021..3950)
                     /locus_tag="WGQ68_000214"
     CDS             complement(3021..3950)
                     /locus_tag="WGQ68_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HEZ0714098.1"
                     /translation="MIHVSAVQAPAHIQDTGRYGHRRYGIGHAGAMDTVALAAGNILL
                     GNDEGTAAIEIALGGIMLVFERDTPFCLTGAVYQAELDGEPVYSYWRYTARKGQTLKM
                     VRAVQGMYGYVCVAGGFDVPEVMGSRSTDLKAGFGGHQGRMLQKGDCLPIGKGAQELS
                     KVGIAPIPFTDTVHLVPSSEYAAFSEKGRLNLERETWTLQSDSNRMGYRFDGQPLTLS
                     QPLEMLSHAVQAGTVQVPPGGKPIILLADAQTTGGYPKIATVAAADLGRLAQVRFGSK
                     VKFKIIGLKEATALRRKNQAYLNQIRRITHEAG"
     gene            complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WGQ68_000215"
     CDS             complement(3947..4594)
                     /gene="pxpB"
                     /locus_tag="WGQ68_000215"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HEZ0714099.1"
                     /translation="MRIEITPISESALVCRLNAPSELGKQQKLWAFAAALGQHDRIEE
                     VVVGMNNLTVFTRFDTDLATLADELQYVWEHTAVTDHQGKLVEIPVCYGGEYGPDLAE
                     VAAFHQTVISEIVRRHTAQTYTVFMMGFQPGFPYLGGLPEALHTPRRAVPRTSVPAGS
                     VGIGGSQTGVYPFASPGGWQIIGRTELPLFRADLNPPTLLAAGDQVRFVAERIEP"
     gene            4778..6985
                     /gene="uvrD"
                     /locus_tag="WGQ68_000216"
     CDS             4778..6985
                     /gene="uvrD"
                     /locus_tag="WGQ68_000216"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HEZ0714100.1"
                     /translation="MFPDQSAPNLLQGLNPEQLSAVTWPPQSALVLAGAGSGKTRVLT
                     TRIAWLLQTGQASVHSIMAVTFTNKAAKEMQTRLGAMIPINVRAMWLGTFHGLCHRFL
                     RLHHRDAGLPSSFQILDGGDQLSLIKRLLKSLNIAEEIIAPRSLQGFINAQKESGLRA
                     SVLSAPDPHTSRMIECYAEYDKICQREGVVDFAELMLRSYEMLQSNEILRQHYQNRFN
                     HILVDEFQDTNKLQYAWLKLMAGGNAAVFAVGDDDQSIYRFRGANVGNMTALMEEFHI
                     DAPVKLEQNYRSVGNILAAANAVIENNDERLGKNLRTDAEAGDKIRYYSAFTDLEEAR
                     FIVDETKALEREGWDLDEIAVLYRSNAQSRVIEQSLFRSGIPYKIYGGLRFYERQEIK
                     HALAYLRLAVNPDDDNALLRVINFPPRGIGARTVENLQTASNEQGITLWQAACNAGAK
                     AAKVVAFVRLIEALRNQVGQMPLSEIIVGILKDSGLTEHYRTQKGDNQDRLDNLDELV
                     NAAIEFKPEDSNFETLPENISDDPAFPILAFLSNATLESGENQAGAGEKAVQLMTVHA
                     AKGLEFNAVFLTGMEEGRFPSEMSLAERGGLEEERRLMYVAITRARKRLYITMAQQRM
                     LHGQTQFGIVSRFVEEIPPEVLHYLSVKKTAYDSYGSPRQTAASKDKIIDDYKQPQTY
                     AGFRIGQNVRHAKFGTGVIIDAADKGESARLTINFGKQGVKELDTKFAKLEAM"
     gene            7342..7881
                     /locus_tag="WGQ68_000217"
     CDS             7342..7881
                     /locus_tag="WGQ68_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0714101.1"
                     /translation="MSQVFKDFDLSSVWESDSWADENYQEAPFTPEILAAVESELGYK
                     LPQSFIELMAVQNGGIFVKNCFPTTQRNSWAENHVQICEVSGIGFEKEGSLCGETGQK
                     LWLEEWEYPPIGVYFANDPSGGHALFALDYRACGKDGEPKVVFVEQESDFEIVELAPD
                     FETFIRSLRHEDEFIDEEI"
     gene            complement(8071..8286)
                     /locus_tag="WGQ68_000218"
     CDS             complement(8071..8286)
                     /locus_tag="WGQ68_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714102.1"
                     /translation="MNIINYTGDDIIISLTREELQLLRSLVIEIYAGVCIDAEEFEIV
                     SGIRNPQLVQELEQHLIEAYNLMDTTG"
     gene            complement(8294..8497)
                     /locus_tag="WGQ68_000219"
     CDS             complement(8294..8497)
                     /locus_tag="WGQ68_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714103.1"
                     /translation="MLKRNLGFDTFKLITIFFLGIRRLQSKHIRAIWDDKSGAIVIRD
                     PNSKDGGTAFRPTLGKTYFDKQK"
     gene            complement(8410..8769)
                     /locus_tag="WGQ68_000220"
     CDS             complement(8410..8769)
                     /locus_tag="WGQ68_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 8 family protein"
                     /protein_id="HEZ0714104.1"
                     /translation="MIKLDLKSINLYDIDFEKFTPEIPDNFHRWIDLDIGIEGEQGSS
                     IFSLCICSPKWISHHCNKEGFFWSNALILEQFDHKIIKSEIDKILEYCSKETWDLTLS
                     NLLRFFSWEFEDYNPNT"
     gene            8822..9199
                     /locus_tag="WGQ68_000221"
     CDS             8822..9199
                     /locus_tag="WGQ68_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0714105.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTATEPDSIVYTDCYRSYDV
                     LDVSEFSHFRINHSTHFAERQNHINGIENFWNQAKRHLRKFNGIPKAHFELYLKECEW
                     RFNNSEIKVQISILKQLVKSSLS"
     gene            9398..10018
                     /locus_tag="WGQ68_000222"
     CDS             9398..10018
                     /locus_tag="WGQ68_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0714106.1"
                     /translation="MNSTASKTLKGLSLVFFASGFCALIYQVSWQRLLFSHIGIDLSS
                     ITVIISVFMVGLGVGAYFGGRIADRFPSSIIPLFCIAEVSIGLFGLVSRGLISGLGHL
                     LVEADLPIIAAANFLLLLLPTFMMGATLPLLTCFFNRKIHNVGESIGTLYFFNTLGAA
                     LGSLAAAEFFYVFFTLSQTIALTACFNLLIAASVWLRYRKDGYSEH"
     gene            10041..11492
                     /locus_tag="WGQ68_000223"
     CDS             10041..11492
                     /locus_tag="WGQ68_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HEZ0714107.1"
                     /translation="MLSFLSGLLSLGIEVLWVRMFSFAAQSVPQAFSFTLACFLTGIA
                     VGAYFGKRICRSRFVDIPFIGQCFLWAGIADFLILGAAWLLTGFSGFVHHAGIFITLS
                     AVVRGLIFPLVHHVGTDGNKSGRQVSNVYFANVAGSALGPVLIGFVILDFLSTQQIYL
                     LICLISAAVPLFCTLFQKSLRLNAVSVAVSLMFGILMFLLPDSVFQNIADRPDRLIEN
                     KHGIVAVYHRDGDKVVYGANVYDGAYNTDVFNSVNGIERAYLLPSLKSGIRRIFVVGL
                     STGSWARVLSAIPEMQSMIVAEINPAYRSLIADEPQIAPLLQDKRVEIVLDDGRKWLR
                     RHPDEKFDLILMNTTWYWRAYSTNLLSAEFLKQVQSHLTPDGIVMFNTTHSPHAFATA
                     VHSIPYAYRYGHMVVGSATPVVFPNKELLKQRLSRLIWPESGRHVFDSSTVDAAAQKV
                     VSRMLIQMTEPSAGAEVITDDNMIVEYKYGRGI"
     gene            11943..12299
                     /locus_tag="WGQ68_000224"
     CDS             11943..12299
                     /locus_tag="WGQ68_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEZ0714108.1"
                     /translation="MLSAYFPVFVFILVGLAAGVLFILLGTILGPKRHYAEKDAPYEC
                     GFEAFENARMKFDVRYYLVAILFILFDLEVAFMLPWAVVFKDLGAYGFWSMLVFIVVL
                     TVGFVYEWKKGALEWE"
     gene            12290..12772
                     /locus_tag="WGQ68_000225"
     CDS             12290..12772
                     /locus_tag="WGQ68_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HEZ0714109.1"
                     /translation="MGIEGVLKKGFITTSADTVLNYMRTGSLWPVTFGLACCAVEMMH
                     AGMARYDLDRFGIIFRPSPRQADLMIVAGTLTNKMAPALRRVYDQLAEPRWVLSMGSC
                     ANGGGYYHYSYSVVRGADRVVPVDVYVPGCPPTAEALIYGLIQLQQKIKRTSTIARDE
                     "
     gene            12785..13378
                     /locus_tag="WGQ68_000226"
     CDS             12785..13378
                     /locus_tag="WGQ68_000226"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEZ0714110.1"
                     /translation="MASIQNLYETVVGVLGDQASKVISALGEITVECLPEHYISVMTA
                     LRDHEELHFELLVDLCGVDYSTYKNEVWQGKRFAVVSQLLSVKNNQRIRVRVWVSDDD
                     FPVVESVVPVYNSADWYEREAFDLYGIMFNNHPDLRRILTDYGFVGHPFRKDFPISGY
                     VEMRYDEEQKRVIYQPVTIEPREITPRIVREENYGGQ"
     gene            13368..14624
                     /gene="nuoD"
                     /locus_tag="WGQ68_000227"
     CDS             13368..14624
                     /gene="nuoD"
                     /locus_tag="WGQ68_000227"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEZ0714111.1"
                     /translation="MANKLRNYTINFGPQHPAAHGVLRMILELEGETIVRADPHIGLL
                     HRGTEKLAETKTYLQALPYMDRLDYVSMMVNEQAYCLAVEKLAGIDVPIRAQYIRVMF
                     AEVTRILNHLMGIGSHAFDIGAMTAILYAFRDREELMDLYEAVSGARMHAAYFRPGGV
                     YRDLPDFMPKYESSKFRNAKVLKQLNESREGTMLDFIDAFCERFPKNIDTLETLLTDN
                     RIWKQRTVGIGVVTPERAMQKGFTGVMLRGSGVEWDVRKKQPYEMYDKMDFDIPVGVN
                     GDCYDRYLCRMEEMRQSVRIIKQCSEWLRVNPGPVITTNHKFAPPKRTEMKTGMEDLI
                     HHFKLFTEGMHVPEGETYTAVEHPKGEFGVYIISDGANKPYRLKIRAPGFAHLQGMDE
                     MAKGHMLADVVAIIGTQDIVFGEVDR"
     gene            14687..15127
                     /locus_tag="WGQ68_000228"
     CDS             14687..15127
                     /locus_tag="WGQ68_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003783540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0714112.1"
                     /translation="MNFWHMQMHPTDEMEFSENIDWILEHKKIIGLGQWEGGKGQIAA
                     FINEMQVNDIVALKRGQELIALVQITGGAYKVTDDANLETAWIVNRRPVRVLDWAIEP
                     KILKQARGTLNRCVRDDVETTQTIKQWYEAVVKSFTKRRVDLTV"
     gene            15181..15654
                     /gene="nuoE"
                     /locus_tag="WGQ68_000229"
     CDS             15181..15654
                     /gene="nuoE"
                     /locus_tag="WGQ68_000229"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HEZ0714113.1"
                     /translation="MLSTESLKQIDIELAKYPADQRRSAIMGALRIAQTEKGWLAPET
                     IAFVADYIGISPAQAYEVATFYNMYDLEPVGKYKLTVCTNLPCALRGGMATGEYLKQR
                     LGIGYGEITPDGKFTLVEGECMGACGDAPVMLVNNHSMCSFMTEEAIEKKLAELE"
     gene            16043..17344
                     /gene="nuoF"
                     /locus_tag="WGQ68_000230"
     CDS             16043..17344
                     /gene="nuoF"
                     /locus_tag="WGQ68_000230"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEZ0714114.1"
                     /translation="MAIYQSGVIFDQVDTANPDCWTLDEYVKRGGYTALRKILSENIS
                     QTDVIDEVKTSGLRGRGGAGFPTGLKWSFMPRSFPGEKYVVCNTDEGEPGTFKDRDII
                     MFNPHALIEGMIIAGYAMGAKAGYNYIHGEIFEGYQRFETALAQARAAGFLGKNILGS
                     DFEFELFAHHGYGAYICGEETALLESLEGKKGQPRFKPPFPASFGLYGKPTTINNTET
                     FSSVPFIIRDGGQAFADKGIPNAGGTKLFCISGHVERPGNYEVPLGTPFAEVLKMAGG
                     MRGGKKLKAVIPGGSSAPVLPADIMMQTNMDYDSISKAGSMLGSGAIIVMDEDVCMVK
                     ALERLSYFYYDESCGQCTPCREGTGWLYRIVHRIVEGKGCMEDLDLLDSIGNQMAGRT
                     ICALADAAVFPVRSFTKHFRDEFVYYIEHGGPMKEHKWGGW"
     gene            17344..17718
                     /locus_tag="WGQ68_000231"
     CDS             17344..17718
                     /locus_tag="WGQ68_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714115.1"
                     /translation="MAEKNYFGYPCDDWEAAKLEAKKYLIEKVAKLGNDITYGNLALA
                     IKTIDIPAYGDAMSNFLDEMSRSTYDAHGFMLSALVVSQKTGEPGKGFWILAKELNGE
                     QKSWGYWYRNEKKKIWEKYKQS"
     gene            17929..18240
                     /locus_tag="WGQ68_000232"
     CDS             17929..18240
                     /locus_tag="WGQ68_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0714116.1"
                     /translation="MQNHPFASQPAVYILANGKNGTLYIGVTMNLPERVWQHKNHVNI
                     DGFTARYDVHDLVWYEFFENMPEAVAKEKTMKKWRREWKIKLIEEQNPEWLDLSGVLF
                     V"
     gene            18522..20783
                     /locus_tag="WGQ68_000233"
     CDS             18522..20783
                     /locus_tag="WGQ68_000233"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HEZ0714117.1"
                     /translation="MLQIEIDGKQVSVEQGATVIEAAHKLGTYIPHFCYHKKLSIAAN
                     CRMCLVDVEKAPKPLPACATPVTDGMIVRTHSAKAREAQEGVMEFLLINHPLDCPTCD
                     QGGECQLQDLAVGYGKTTSRYTEEKRSVVGKDMGPLVSAEEMSRCIHCTRCVRFTEEI
                     AGLQEIAMVNRGEHSEIMPFIGKAVETELSGNVIDLCPVGALTSKPFRFNARTWELNR
                     RKSVSAHDALGSNLIVQTKDHTVRRVLPLENEAINECWLSDRDRFAYEGLYHESRLKN
                     PKIKQGGEWMDVDWKTALEYVRSAIECIAKDGNQNQVGIWANPMNTVEELYLAKKLAD
                     GLGVKNFATRLRQQDKRLSDGIKGAQWLGQSIESLADNDAVLVVGANLRKEQPLLTAR
                     LRRAAKDRMALSVLAGSKEELFMPLLSQEAAHPDEWAGRLKNLSANAEHAVTASLKNA
                     EKAAVILGAEVQNHPDYAAIYAAVQELADATGAVLGILPQAANSVGADVLGVNSGESV
                     AEMANAPKQAVLLLNVEPEIDTVDGAKAVAALKQAKSVMAFTPFVSETLLDVCDVLLP
                     IAPFTETSGSFINMEGRLQSFHGVVQGFGDSRPMWKVLRVLGNLFDLKGFEYHDTVAI
                     LKDALDAESLPSKLNNRAASTQKDFQTASSRLVRVGGVGIYHTDAIVRRSAPLQETSH
                     AAVPAARVNPNTLARLGLQDGQTAVAKQNGASVSVAVKADAGLPENVVHLPLHTENAA
                     LGALMDTIELAGA"
     gene            20786..21862
                     /gene="nuoH"
                     /locus_tag="WGQ68_000234"
     CDS             20786..21862
                     /gene="nuoH"
                     /locus_tag="WGQ68_000234"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HEZ0714118.1"
                     /translation="MQEWFQNLFAATLGLGDLGITVGLVVSVIVKIVIILIPLILTVA
                     YLTYFERKVIGFMQLRVGPNVTGPWGLIQPFADVFKLLFKEVTRPRLSNKALFYIGPI
                     MSLAPSFAAWAVIPFNEEWVLTNINIGLLYILMITSLSVYGVIIAGWASNSKYSFLGA
                     MRASAQSISYEIAMSAALVCVVMVSGSMNFSDIVAAQAKGIAGGSVFSWNWLPLFPIF
                     IVYLISAVAETNRAPFDVAEGESEIVAGHHVEYSGFAFALFFLAEYIFMILISALTSL
                     MFLGGWLSPFPQSWGFIGTPSAFWMFVKMAAVLYWYLWIRATFPRYRYDQIMRLGWKV
                     LIPIGFAYIVVLGVWMISPLNLWK"
     gene            21943..22422
                     /gene="nuoI"
                     /locus_tag="WGQ68_000235"
     CDS             21943..22422
                     /gene="nuoI"
                     /locus_tag="WGQ68_000235"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HEZ0714119.1"
                     /translation="MANLVKTFLLGELVKGMGVTLKNFFARKDTIYFPEEKTPQSVRF
                     RGLHAQRRYPNGEERCIACKLCEAVCPAMAINIESEEREDGTRRTKRYDIDLTKCIFC
                     GFCEEACPTDAIVETHIFEYHGEKKGDLHMTKPILLAIGDKYEAEIAKRKAADAPYR"
     gene            22434..23105
                     /locus_tag="WGQ68_000236"
     CDS             22434..23105
                     /locus_tag="WGQ68_000236"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEZ0714120.1"
                     /translation="MTFSTILFYILAAIVLYGAVRTVTAKNPVHAALHLVLTFCVSAM
                     IWMLMQAEFLGVTLVVVYVGAVMVLFLFVVMMLNIDIEEMRAGFWRHAPVAGVVGTLL
                     AVALILILVNPKTNLAAFGLMKDIPADYNNIRDLGSRIYTDYLLPFELAAVLLLLGMV
                     AAIALVHRKTVNPKRMDPADQVKVRADQGRMRLVKMEAVKPQVESAEESEVSDGLKPE
                     GEGKA"
     gene            23102..23407
                     /gene="nuoK"
                     /locus_tag="WGQ68_000237"
     CDS             23102..23407
                     /gene="nuoK"
                     /locus_tag="WGQ68_000237"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEZ0714121.1"
                     /translation="MITLTHYLVLGALLFGISAMGIFMNRKNVLVLMMSIELMLLAVN
                     FNFIAFSQHLGDTAGQIFVFFVLTVAAAESAIGLAIMVLVYRNRQTIDVADLDGLKG"
     gene            23441..24016
                     /locus_tag="WGQ68_000238"
     CDS             23441..24016
                     /locus_tag="WGQ68_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine monophosphate-protein transferase
                     NmFic"
                     /protein_id="HEZ0714122.1"
                     /translation="MPSENPIGKTMKSIDEQSLHNARRLFESGDIDRIEVGTTAGLQQ
                     IHRYLFGGLYDFAGQIREDNISKGGFRFANAMYLKEALVKIEQMPERTFEEIIAKYVE
                     MNIAHPFLEGNGRSTRIWLDLVLKKNLKKVVNWQNVSKTLYLQAMERSPVNDLELRFL
                     LKDNLTDDVDNREIIFKGIEQSYYYEGYEKG"
     gene            24140..24481
                     /locus_tag="WGQ68_000239"
     CDS             24140..24481
                     /locus_tag="WGQ68_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714123.1"
                     /translation="MMDKNQLEQEFHKAMLNIYQEALNLPQPYKATRFLQIVNEFGGK
                     EAADKLLSTGEKKTQTGFTELILSGGGVHALKYSMEYLVLQKPWCDLFTEEQLAVARK
                     RLERVGFVFPK"
     gene            24530..26554
                     /gene="nuoL"
                     /locus_tag="WGQ68_000240"
     CDS             24530..26554
                     /gene="nuoL"
                     /locus_tag="WGQ68_000240"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEZ0714124.1"
                     /translation="MNDMTLYLIIALVPLAGSLIAGLFGNKIGRAGAHTVTILGVAVS
                     AVLSAYVLWGFIDGSRAKFDENVYTWLTMGGLDFSVGFLVDTMTAMMMVVVTGVSLMV
                     HIYTIGYMHDEKVGYQRFFSYISLFTFSMLMLIMSNNFIQLFFGWEAVGLVSYLLIGF
                     YFKRPSATFANLKAFLINRVGDFGFLLGIGLVLAYFGGSLRYQDVFAYLPNVQNATIQ
                     LFPGVEWSLITVTCLLLFVGAMGKSAQFPLHVWLPDSMEGPTPISALIHAATMVTAGL
                     FMVSRMSPIYEMSSTALSVIMVIGAITALFMGFLGVIQNDIKRVVAYSTLSQLGYMTV
                     ALGASAYSVAMFHVMTHAFFKALLFLAAGSAIIGMHHDQDMRHMGNLKKYMPVTWLTM
                     LIGNLSLIGTPFFSGFYSKDSIIEAAKYSTLPGSGFAYFAVLASVFVTAFYAFRQYFM
                     VFHGEEKWRSLPEHHSDGHGEEHHGLGKNDNPHESPLVVTLPLILLAVPSVIIGYIAI
                     EPMLYGDFFKDVIFVNADAHPTMHIMKEEFHGALAMVSHSLHSPVLYLAIAGVLSAWL
                     LYVKLPHLPAKIAQAFRPVYVLFENKYYLDALYFNVFAKGTRALGTFFWKVGDTAIID
                     NGIVNGSARLVGAVAAQVRKVQTGFIYTYAAAMVFGVLVLLGMTFWGLFR"
     gene            26610..26930
                     /locus_tag="WGQ68_000241"
     CDS             26610..26930
                     /locus_tag="WGQ68_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HEZ0714125.1"
                     /translation="MSNTQTIRSADFTTSRAWGALDIANMNGTTVRLHWTDQPYKWHV
                     NDGEEVFAVMDGEVDMHYRENGEEHIVRLKSGDIFYAGIGAEHVAHPRGETRILVIEK
                     EGSV"
     gene            27005..28720
                     /locus_tag="WGQ68_000242"
     CDS             27005..28720
                     /locus_tag="WGQ68_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HEZ0714126.1"
                     /translation="MANLNVSSKKIINLLGNQDMQEKYFIIPEYQRPYRWDTEKCAVL
                     WSDIVDFRQSSTGKNEEYFLGTIVVCPNEKGNLEVIDGQQRLTSLLLLLRAFYRVLEQ
                     TESTPDTAAKITTLKSKIAPCIWDVDVLTAEITDRTAIRLHTQVATETDNEILHEILA
                     NGEISEECRSNYAVNYLYFLKQCQEYARTNPMDWYHLCLTILNDCILLPIESDSLNSA
                     LTIFSTLNDRGLPLSDADIFKARLYKMAQQKDKFVEQWKELVALAEGASISVDDLFRY
                     YTHVLRAKEKITDKEIGLRRFYTDNRNKQLENENLFNHLIELAGFWQDINNSKTNQDV
                     ETSNFVNDEAAKWLQCLNVYPNEFWKYAASVFFIANKGAQSFKDDFAALLKQLTAFLY
                     AKFLVAPTVTAIKDDIYKLCVAIYHGEKNALSYVMPAPIHYEQNTGRITKGLLMINAY
                     LFDEKQKLLPTKTEIEHIFPKKWQETNYNGWSIIDAEQYLEQLGNKIVFEKRLNIQAG
                     NGYFGKKKEQYQKSDIVEVRELAGENANDWLPSDIQKRQQQIVDRLKQFFDNCFEAAS
                     IPKKN"
     gene            28757..30253
                     /locus_tag="WGQ68_000243"
     CDS             28757..30253
                     /locus_tag="WGQ68_000243"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEZ0714127.1"
                     /translation="MFSNHLLSLAIWIPIAAGVLVLATGKDSRAPLARVLAFMGALAG
                     FLVTLPLFTGFDRLSGGYQFTEFHEWIPLLKINYALGVDGISVLFIILNAFITLLVVL
                     AGWEVIQKRPAQYMAAFLMMSGLINGAFAAQDAILFYVFFEGMLIPLYLIIGVWGGPR
                     RVYASVKLFLYTLMGSLLMLVAMVYLYYQTGSFSIVDFQNIKQIPLGVQQLLFAAFFL
                     SFAVKVPMFPVHTWLPDAHVEAPTGGSMVLAAITLKLGAYGFLRFILPIMPDAARYFA
                     PVIIVLSLIAVIYIGMVALVQTDMKKLVAYSSISHMGFVTLGMFLFVDGQLDDWALKG
                     AIIQMISHGFVSAAMFMCIGVMYDRLHTRNIADYGGVVNVMPKFAAFMMLFGMANAGL
                     PATSGFVGEFMVIMGAVKVNFWVGALAAMTLIYGASYTLWMYKRVIFGAIHNPHVAEM
                     KDINCREFAILAVLAVAVLGMGLYPNAFIEVVHQAANDLIAHVAQSKM"
     gene            30263..31708
                     /gene="nuoN"
                     /locus_tag="WGQ68_000244"
     CDS             30263..31708
                     /gene="nuoN"
                     /locus_tag="WGQ68_000244"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEZ0714128.1"
                     /translation="MNWSDLNLMPAMPEIVLLALLVLLLLADLWVSDDKRRWTHYGAL
                     ATVAVTAVVQLAVWEQGSTSSFNGMYIADGMSRLAKMVLYALTFALFVYAKPYNQVRG
                     IFKGEFYTLSLFALLGMSVMVSAGHFLTAYIGLELLSLALYALIALRRDSGFAAEAAL
                     KYFVLGALASGLLLYGISMVYGATGSLEFAGVLASSFNEEANEWLLKLGLVFIVVAVA
                     FKLGAVPFHMWVPDVYHGAPTSVTALVGTAPKIAAVVFAFRILVTGLGTVHHDWSLMF
                     ALLAAASLLVGNLAAIMQTNIKRMLAYSTVSHMGFILLAFMAGAVGFAAGLYYAITYA
                     LMAAAGFGVLMVLSDGDNECENISDLAGLNQHRVWLAFLMLLVMFSMAGIPPLMGFYA
                     KFGVIMALLKQGYVWLSVFAVIMSLIGAFYYLRVVKVMYFDESGRAHPTVGGNNAAKF
                     LLSVNALLLVLWGIMPQTVIDWCAKALENTL"
     gene            31820..32119
                     /locus_tag="WGQ68_000245"
     CDS             31820..32119
                     /locus_tag="WGQ68_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2818 family protein"
                     /protein_id="HEZ0714129.1"
                     /translation="MTASMYILLLLALIFANAPFLTTKLFGIVPLKRKHFGHHLIELA
                     AGFALTAVLAYILESRAGAVHDQGWEFYATVVCLYLIFAFPCFVWRYFWHTRNRE"
     gene            complement(32228..33124)
                     /locus_tag="WGQ68_000246"
     CDS             complement(32228..33124)
                     /locus_tag="WGQ68_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEZ0714130.1"
                     /translation="MNPTNDLKAWQQRAQAQTELLLERFLPSENEIPHTLHEAMRYAA
                     LDGGKRLRPMLVLAASELGGVVHEAVEQAMAAIEMIHVYSLVHDDMPAMDNDSLRRGK
                     PTCHVQYDEATALLVGDALQTQAFDVLSRPTELPAARQLAMLSVLAKAGGSAGMAGGQ
                     AIDLANVGKQMVQADLEQMHSLKTGALIRAAVLLGATACPDLSDAELSVLDAYAAKLG
                     LAFQVIDDVLDCEADTATLGKTAGKDADNDKPTYVKLMGLEAARSYAHKLVAEAVALL
                     EPFGDKALRLRQLAEFAVARKY"
     gene            complement(33108..33332)
                     /locus_tag="WGQ68_000247"
     CDS             complement(33108..33332)
                     /locus_tag="WGQ68_000247"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEZ0714131.1"
                     /translation="MKKNAPKSFEEALSRLESLTQAMQGEMPLEDALAAYQEGNELVR
                     YCQTKLAQVEQKLQVLDADGLKELNLESDE"
     gene            complement(33393..33569)
                     /locus_tag="WGQ68_000248"
     CDS             complement(33393..33569)
                     /locus_tag="WGQ68_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII"
                     /protein_id="HEZ0714132.1"
                     /translation="MPSETGFGFQTASVFQKCYNPLFTGTSETMFQHAGRHLKHRPMC
                     RPDLEGLTPLPNKV"
     gene            complement(33596..34519)
                     /gene="rsgA"
                     /locus_tag="WGQ68_000249"
     CDS             complement(33596..34519)
                     /gene="rsgA"
                     /locus_tag="WGQ68_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEZ0714133.1"
                     /translation="MPSEHPFSDGISTPNPKETMNDTAQITASYGRRYIVRTPDGTTY
                     EASTRKKRVDFACGDRVRISPVNAEQVVIEDFLPRQSLLYRQDAWKTKLIAANVTQLL
                     IVTAAVPSPSVRLLQRALLAAEAAGIRAVIVLNKADLPETALWREKLKFYETLGYPVI
                     ETRALENAGSLRPALQGHSNILLGQSGMGKSTLTNALLGSQTARTGDISAALDSGKHT
                     TTHARLYDLNGETQLIDSPGLQEFGLHHLQAADLPRYFPDFRHLVGQCRFHNCTHRAE
                     PGCAFKAAAETRAASPERLAFLQGITDELPG"
     gene            complement(34524..36380)
                     /locus_tag="WGQ68_000250"
     CDS             complement(34524..36380)
                     /locus_tag="WGQ68_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714134.1"
                     /translation="MLNKIFSWFESRIDPYPEAAPKTPEKGLWRFVWSSMTGVRKWIA
                     ALAALTAGIGIMEALVFQFMGKIVEWLGKYAPAELFAEKGWELAAMAAMMVFSVAWAF
                     AASNVRLQTLQGVFPMRLRWNFHRLMLNQSLGFYQDEFAGRVSAKVMQTALALRDAVM
                     TVADMVVYVSVYFITSGVILASLDSWLLLPFIGWIVGFASVMRLLIPKLGQTAAWQAD
                     ARSLMTGRITDAYSNIATVKLFSHGAREAAYAKQSMEEFMVTVRAQMRLATLLHSCSF
                     IVNTSLTLSTAALGIWLWHNGQVGVGAVATATAMALRVNGLSQYIMWESARLFENIGT
                     VGDGMATLSKPHTILDKPNALPLKVPQGAIKFEHVDFSYEAGKPLLNGFNLTIRPGEK
                     VGLIGRSGAGKSTIVNLLLRFYEPQSGTVSIDGQDISGITQESLRAQIGLVTQDTSLL
                     HRSVRDNIIYGRPDATDAEMVSAAERAEAAGFIPELSDAKGRRGYDAHVGERGVKLSG
                     GQRQRIAIARVMLKDAPILLLDEATSALDSEVEAAIQESLDKMMDGKTVIAIAHRLST
                     IAAMDRLVVLDKGRIIEEGTHAELLEKRGLYAKLWAHQSGGFLNEHVEWQHD"
     gene            complement(36406..36543)
                     /locus_tag="WGQ68_000251"
     CDS             complement(36406..36543)
                     /locus_tag="WGQ68_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714135.1"
                     /translation="MPSEGGATFALTKPCVSASRCLPDGFSRKMALLSFLSDGIVFDA
                     V"
     gene            complement(36543..37127)
                     /gene="ruvA"
                     /locus_tag="WGQ68_000252"
     CDS             complement(36543..37127)
                     /gene="ruvA"
                     /locus_tag="WGQ68_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEZ0714136.1"
                     /translation="MISRLTGKLVEKNPPQIVIDVNGVGYEADVSMQTFYNLPPVGES
                     VQLFTQLIIREDAHLLFGFATAEERKTFRQLIKVSGIGAKTALGILSAMTADELAQAV
                     AEEDVKRLSSAPGIGKKTAERMVLELRGKLVAHAVTDGLFAAAPAADETEDIVGTLLA
                     LGYSEREAKAAVKGVPKGTDVGEGVRLALKNLLK"
     gene            complement(37209..37535)
                     /locus_tag="WGQ68_000253"
     CDS             complement(37209..37535)
                     /locus_tag="WGQ68_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0714137.1"
                     /translation="MNFKRLLLTAAATALMGISAPALAHHDGHGDDDHGHAAHQHSKQ
                     DKIISRAQAEKAALARVGGKITDIDLEHDNGRPHYDVEIVKNGQEYKVVVDARTGRVI
                     SSRRDD"
     gene            complement(37589..38308)
                     /locus_tag="WGQ68_000254"
     CDS             complement(37589..38308)
                     /locus_tag="WGQ68_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HEZ0714138.1"
                     /translation="MTLTRKTLFLLTAAFGTHSLQTASADAVVKAEKLHASANRSYKV
                     AGKRYTPKNQVAEFTQTGNASWYGGRFHGRKTSGGERYDMNAFTAAHKTLPIPSYVRV
                     TNTKNGKSVIVRVNDRGPFHGNRIIDVSKAAAQKLGFVSQGTAHVKIEQIVPGKSAPV
                     AENKDIFIDLKSFGTEHEAQAYLNQAAQNFAASTSSPNLAIEKRRYEYVVKMGPFASQ
                     ERAAEAEAQARGMIQTVLTAG"
     gene            complement(38305..38427)
                     /locus_tag="WGQ68_000255"
     CDS             complement(38305..38427)
                     /locus_tag="WGQ68_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEZ0714139.1"
                     /translation="MPSENLSGHIPNRRPHCTAVRNVSGGHDAHSSSTQERNTF"
     gene            complement(38438..38902)
                     /gene="trmL"
                     /locus_tag="WGQ68_000256"
     CDS             complement(38438..38902)
                     /gene="trmL"
                     /locus_tag="WGQ68_000256"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEZ0714140.1"
                     /translation="MFTIVLYQPEIPPNTGNIIRLCANTGADLHLVKPLGFPLDSAKM
                     KRAGLDYHEFASLTVYENFDDCLKALAGRRIFALTTKGTARPDETAFQKGDVLLFGPE
                     TRGLPADILDSLPAAQKIRLPMQPGSRSMNLSNTVAVILFEAWRQHGYAGGV"
     gene            complement(38967..39464)
                     /locus_tag="WGQ68_000257"
     CDS             complement(38967..39464)
                     /locus_tag="WGQ68_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEZ0714141.1"
                     /translation="MWASLHYEPPVSNMIHALKHLADLSMVQPLADLMMQNPPDRLAD
                     ECFDFVLPVPLSRERLLQRGFNQSESIVGLLAQRYGWQILPRHTVFRHHRPPQSTLKG
                     GERRRNIKNAFEIRTPIPENCNILLIDDVFTTGATLDELAKTLKKLGANRICCWTLAR
                     TPMKK"
     gene            39520..40434
                     /gene="bioH"
                     /locus_tag="WGQ68_000258"
     CDS             39520..40434
                     /gene="bioH"
                     /locus_tag="WGQ68_000258"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HEZ0714142.1"
                     /translation="MCLKHRGQLFAASVRYAAKSLRQPAQMPSETPDEPWHNTQRLIV
                     GASAMRRQQERKSMPDAVKKVYLIHGWGANRHMFDDLMPRLPATWPVSAVDLPGHGDA
                     PFAQPFDIEAAADAVAAQIDTPADILGWSLGGLVALYLAARHPDKVRSLCLTASFARL
                     TADEDYPEGLAAPALGKMVGAFRSDYAKHIKQFLQLQLLHTPDADGIIGRILPDLARC
                     GTPSALQEALDAAERADARHLLDKIDVPVLLVFGGKDAITPPRMGEYLHRHLKGSRLV
                     VMEKAAHAPFLSHAEAFAALYRDFVEGV"
     gene            40436..41224
                     /locus_tag="WGQ68_000259"
     CDS             40436..41224
                     /locus_tag="WGQ68_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEZ0714143.1"
                     /translation="MNPQDKCWQVHRHLAEYADQRLTLVRNAPKYILLAGADADISRS
                     LLAKRYPQAVFEEYDSRADFLAAAAAARKGGFWQRFTGKGVVQHCQSPIAPLPEACAD
                     MLWSNLGLLAAEQILPVLHNWACALKTDGLLFFTCFGRDTFTELKCRLKENGIESRSV
                     MFPDMHDLGDMLAENGFYDPVTDTAKLVLDYKKAETFWADMDTLGIWRAMAWNDENAA
                     RSCVGTIFEREGGLGITLETVYGHAVKKLMLPQGENVVQFFPKR"
     gene            41445..41634
                     /locus_tag="WGQ68_000260"
                     /pseudo
     CDS             41445..41634
                     /locus_tag="WGQ68_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239468.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            41655..>42040
                     /locus_tag="WGQ68_000261"
     CDS             41655..>42040
                     /locus_tag="WGQ68_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein"
                     /protein_id="HEZ0714144.1"
                     /translation="MYWERGLHMYKASAVVPTGYVRVGNTAPLVGEDTQQYASFWGDG
                     YDVYRQLRWQQIPEKQRKAFKKAAKSKKTVMFAGREYGISKQNLSDVWDDFEDAMELK
                     AFPCLSSLFLTKWHKNLYDSGLTKTST"
BASE COUNT         9818 a        10571 c        11229 g        10422 t
ORIGIN      
        1 cctctttcag ttacagcagc ttgatccctt tcccttatcc aacgggggaa ggctaggata
       61 gggtggcttg caaatataca gaacaaggga caagagccac cctctctcca accctctccc
      121 tccgtacggg agggggtgga ttctcgcggg cgaagcccac gctacggtta gcctttaccc
      181 cagcacaaac aattcccgcc cgtgcgcctt cagccaactt ttagcattgt cggtatgcgg
      241 cgtcagcgtg ttcaccaaat gccaaaagcg cggactgtgg tcggggtggc ggaggtggca
      301 gagttcgtgg atgcagacat agtcggcaac gtacttgggc gcgccgatca gccgccagtt
      361 gaggcggatg ccggtgtgcg ggcggcatac gccccaaaag gttttggcgt tgctcaggtc
      421 tgtggcggtg ggcgtcagtc ctgtttcggc tgcgtgtttt tcaaggcggg gcagcaggta
      481 ttcgcgggcg cgttcgttca acaggcggcg caggtggtcg atttgtgcgg cggtttcttt
      541 tcggggaagc aggatttcag acgacgtgat acggatatgg ctttggctgt gggtatccag
      601 cttggtcttt attccccgat accaaatcca ctcgggtaag tttgggtggg aaacaggatg
      661 cacgggcgtt ttggcaagcg tgttccgcaa aatcgtttcg tttgccgcca gccagtttgc
      721 taacgcgtgg tcttgaaaaa agggtgggac gttgatgctg accgtctgca tattgacggg
      781 gcgcagaatc agatttttct tggcactgcg tttgagttcg atttcgatgc acaaaccgtc
      841 ggaaagagta taggtgaagc gtttcatagt tgtgaatagg tttcagaccg gatacatcgt
      901 ctgaaacagg aattttccat atcaggcggc aaacttcgga taatatacaa aatcaaacat
      961 ctgcgctaca aggttcagcc gaacaagccg ccgatatatt tgctgatggt aatggcgctg
     1021 agtactgcca tcaaaccgac cacaatcacg ccggaaacgg tgagccacag cgggtgtttg
     1081 tagtcgccga caattttggt tttgtaggcg gcaatcagaa tcagaccgag ggaaatcggt
     1141 aaaatcaggc cgtttaatgc gcctacgaac accagcacct gcgccggttt gccgatggtg
     1201 gaaaatacgg cggtggacac ggcgataaag gcaataatcc atttgttttt attgcgttcg
     1261 atagacgggc tgagaccgga gaagaacgac accgaagtat aagccgcacc aatcaccgaa
     1321 gtaatcgaag ccgcccaaat caccacgccg aaaatcagca ggccgatgta tcccgccgca
     1381 tattcaaacg gtgtggaagc agggttgtcg ggattgagct gtacgccttg gctgaccacg
     1441 cccaaaaccg ccaaaaacaa tacaatccgc ataatcgagg caatcaggat cgcccgcacc
     1501 gagctttggc tcacttccgg caacgccgat ttgcctttga tgcctgcgtc cagcagacgg
     1561 tgcgcaccgg cgaaggtgat gtagccgccg accgtgccgc ccaccagtgt aacaatcgcc
     1621 attgcatcga gtttttccgg cataaaggta tgcacggcgg catctgccag cggcggattc
     1681 gcctgccatg ccacataaac cgtcagcgca atcattacga aacccatcac ttgggcgaat
     1741 ttgtccatca ctttgcctgc ttctttaaac agaaacacac cgatggcaat cacgccgctg
     1801 atcacggcac cggtttccgg cgacagtccg gtcagcaggt tcagacccaa gcctgcgccg
     1861 ccgacgttgc caatattgaa cgccaaaccg cccatcacaa tcagcacagc caagaaatag
     1921 cctgcgccgg gcaagacctg attggcaata tcctgcgcct gtttttcgga aacggcgaca
     1981 atccgccaaa tattgagctg cgccccgatg tcgagcagaa tcgagagcag aatcacaaag
     2041 ccgaaacttg ccgccagtgc ttgggtgaag gtggcggttt gggtcagaaa gcccgggccg
     2101 atggcggaag tcgccatcag gaatgcagcg ccgattaagg catttctgcg gtttttttga
     2161 ttagacataa tcgcttatcc tctataaaat tggttgttgc tgtgtttggg cgaaacctgc
     2221 ggttttagct acgcagaaac tcgctttgct cgttttggcg aaacctgcgg ttttcagacg
     2281 gcctatgaac tgtttttcaa gcagaaactt tgatgcctgc cgccagtagt tcctgccgga
     2341 ttttttcggc aaacaccacg gcgtgcggcc cgtctccgtg cagacagatg ctgtcggctt
     2401 gcacggcaac caggctgccg ttcactgctt tgacccgccc gtcccgcacc atctgcaata
     2461 cttgggcgat ggcttcttcg tcgctgtcca cctgcgcatc ggggcggctg cggggaacca
     2521 gcgtaccgtc gggcatatag cggcggtcgg cgaatacttc ggaaatcaca cccaagcctg
     2581 cggcttttcc ggcttccaag agcaggctgc cggaaagtgc catcaatttc aatttcgggt
     2641 cgaaatccgc cacaattcgg gcaacggtat ccgccagcgc acggtttttc gccgcttggt
     2701 tgtacattgc gccgtgcggt ttgacataag ccatttccaa accctgatca cggcacaagg
     2761 cctgcaatgc gcccaactgg taattcagac acgcccgcaa atcggcttcg gacagattca
     2821 tttcggtacg gccgaagttt tcccgatcgg gatagccggg gtgtgctccg atgcgcacgc
     2881 cgttttgttg ggcatacgcc aatgccgccc gaatatcggc aatgctgccg gcgtgttggg
     2941 cgcaggcgat gttggccgaa gtaatcagct gcaacaaggc ttcgtcgctg ccgcagcctt
     3001 cggcgagatc ggcgtttaaa tcaacctgct tcatgggtga ttctccgtat ttggttcaga
     3061 taggcttggt ttttgcgccg cagggcggtg gcttctttca agccgattat tttgaatttg
     3121 actttgctgc cgaagcgcac ctgtgccagc ctgcccaaat cggcggcggc aacggtagcg
     3181 attttcggat aaccgccggt ggtttgtgca tcggccagca ggataatcgg tttgccgccg
     3241 ggcggcacct gcacggttcc tgcctgaaca gcgtgggaca gcatttccaa aggttgcgac
     3301 agggtcagcg gctgtccgtc gaagcggtag cccatgcggt tgctatcgct ttgcagcgtc
     3361 cacgtttccc gttccagatt cagacgccct ttttcactga aagcggcata ttccgacgaa
     3421 ggaacaaggt ggacggtatc ggtaaacggt atcggggcaa tgccgacttt ggacaattcc
     3481 tgcgcacctt tgccgatggg gagacaatca cctttttgca gcattctgcc ctgatggccg
     3541 ccgaaaccgg ctttcaggtc ggtgcttctc gaacccatca cttccggcac atcaaatccg
     3601 cccgccacgc acacatagcc gtacatgccc tgcacggcac gcaccatttt caaggtctgc
     3661 cctttgcggg cggtataacg ccaatacgaa tagaccggtt cgccgtccaa ttccgcctga
     3721 tacacggcac cggtgagaca aaacggcgta tcccgttcaa acaccagcat tatcccgccc
     3781 aaagcgattt cgattgcggc cgtgccttcg tcgttgccca ataaaatatt acccgccgcc
     3841 aaagcaaccg tgtccatcgc accggcatga ccgatgccgt aacgccggtg tccgtagcgt
     3901 ccggtatcct gaatatgcgc cggtgcctgc actgccgaaa cgtgaatcat ggctcaatcc
     3961 tttctgcaac aaagcggact tggtcacccg ccgccagcag ggtcggcgga ttcaaatcgg
     4021 ctcggaacaa gggtaattcg gttctgccga taatctgcca gccgccgggc gaagcgaacg
     4081 gatacacacc ggtctgactg ccgccgatac cgaccgaacc ggcaggaacg gacgttctcg
     4141 gcacggcacg gcggggcgtg tgcaatgctt cgggcaagcc gcccagataa gggaaaccgg
     4201 gctggaagcc catcataaat acggtataag tttgcgccgt atggcggcgg acgatttcgg
     4261 aaataaccgt ctgatggaaa gcagcgactt ccgccaaatc cgggccgtat tcgccgccgt
     4321 agcagacggg aatttccacc agtttgccct gatggtctgt aacggcggtg tgttcccaca
     4381 catattgcaa ttcatcggca agcgtcgcca aatcggtatc gaaacgggtg aacacggtca
     4441 gattgttcat gccgaccacc acttcctcaa tcctgtcgtg ctgcccgagc gcagcggcaa
     4501 acgcccacaa cttttgctgt ttgcccagtt cggaaggcgc attcagtcgg cagaccaaag
     4561 cggattcgct gattggtgtg atctctattc tcatttgttg ttcattttgg ttatgtttta
     4621 atgaatctat atgcaggggc ggcggtttgt caatatcttc tgtgctgcat catcaaaccg
     4681 tcgattggaa aagtgctgcc ctgccgctgc actttttcag acgaccttaa accgtttcta
     4741 ttaaaatagc gcattccact tttcagacgg catccttatg tttcccgacc aatccgcccc
     4801 caacctgctg caaggcttga atcccgaaca actctccgcc gtaacctggc cgccgcaatc
     4861 cgcccttgtg ctggcgggcg cgggcagcgg caaaacgcgc gtgctgacca cgcgcatcgc
     4921 atggctgttg caaaccggtc aggcaagcgt gcacagcatt atggcggtaa cgtttaccaa
     4981 caaagccgcc aaagaaatgc aaacccgact cggcgcgatg attcccatca acgtccgcgc
     5041 catgtggctc ggcacgttcc acggtctctg ccaccgcttt ttgcgcctgc accaccgcga
     5101 cgccggtctg ccgtcttcct ttcaaatcct cgacggcggc gaccagcttt ccctcatcaa
     5161 acgcctgctc aaaagcctca acatcgccga agaaatcatc gcgccgcgtt cgctgcaagg
     5221 ctttatcaac gcgcaaaaag aatccggctt gcgcgcttcc gtcttgagcg cgcccgaccc
     5281 gcacacaagc cgcatgatcg aatgctacgc cgaatacgac aaaatctgcc aacgcgaagg
     5341 cgtggtcgat tttgccgaac tcatgctccg cagctacgaa atgctgcaaa gcaacgaaat
     5401 cctgcgccaa cactaccaaa accgcttcaa tcacattctg gtcgacgaat tccaagacac
     5461 caacaaactg caatacgcct ggctcaaact catggcgggc ggcaacgcgg cggtatttgc
     5521 cgtcggcgac gacgaccaaa gcatctaccg tttccgtggc gcaaacgttg gcaatatgac
     5581 cgcgctgatg gaagaattcc acatcgacgc gcccgtcaaa ctcgaacaaa actaccgctc
     5641 cgtcggcaac atccttgccg ccgccaatgc cgttatcgaa aacaacgacg aacgactcgg
     5701 caaaaacctg cgcaccgacg ccgaagcagg cgacaaaatt cgctactact ccgcctttac
     5761 cgacctcgaa gaagcccggt tcatcgtgga cgaaaccaaa gccctcgaac gcgaaggctg
     5821 ggatttggac gaaatcgccg tcctctaccg tagcaacgcc caatcccgcg ttatcgaaca
     5881 aagcctgttc cgcagcggca tcccctacaa aatctacggc ggcttgcgct tttacgaacg
     5941 ccaagaaatc aaacacgccc tcgcctacct gcgcctcgcc gtcaatcccg acgacgacaa
     6001 cgccctcttg cgtgtcatca acttcccacc gcgcggcatc ggtgcacgta ccgtcgaaaa
     6061 tcttcagacg gcctcaaacg aacaaggcat caccctctgg caagccgcct gcaacgccgg
     6121 cgcgaaagcc gccaaagtcg tcgccttcgt ccgcctgatt gaagccctgc gcaaccaagt
     6181 cggacaaatg cccctgtccg aaatcatcgt cggcatcctc aaagacagtg gcttgaccga
     6241 acactaccgc acccaaaaag gcgacaacca agaccgcctc gacaaccttg acgaactcgt
     6301 caacgccgcc atcgagttca aaccagaaga cagcaacttt gaaaccctgc ctgaaaacat
     6361 ttcagacgat cccgccttcc ccatcctcgc tttcttgagc aacgccaccc tcgaatccgg
     6421 cgaaaatcaa gcaggtgcag gcgaaaaagc cgtccaactc atgaccgtcc acgccgccaa
     6481 aggcttggaa ttcaacgccg tcttcctcac cggcatggaa gaaggccgct tccccagcga
     6541 aatgagcctt gccgaacgcg gcggcctcga agaagaacgc cgcctcatgt acgtcgccat
     6601 cacccgcgcc cgcaaacgcc tctacatcac catggcacag caacgcatgc tgcacggaca
     6661 aacccaattc ggcatcgtct cccgcttcgt cgaagaaatc ccacccgaag tattgcacta
     6721 cctgtccgtc aaaaagaccg cctacgacag ctacggcagc ccgcgccaaa ccgccgcgtc
     6781 caaagacaaa atcatcgacg actacaaaca gccccaaacc tacgcaggct tccgtatcgg
     6841 acaaaacgtc cgccacgcca aattcggcac tggcgtgatt atcgatgccg cagacaaagg
     6901 cgaatccgcc cgactgacca tcaatttcgg caaacagggc gtgaaagagt tggataccaa
     6961 gtttgcgaaa ttggaagcga tgtaaatttg aaatgcaggc tggatactcg tatccgacaa
     7021 aaatatttga cgcgtctatc gtttccgaaa aaccgctgtt ggaaaatgtc ggattcgaga
     7081 atccgaccta tggcaaaaaa ctgtagcaag gacaaggcaa aaggccgtct gaatccggta
     7141 acaacaaatt ttgccgaaac ctccgccgtc attcccgcgc aggcgggaat ccagaccttt
     7201 cggcacggaa acttatcgga taaaaggttt ctttagattc cacgttctag attcccgcct
     7261 gcgcgggaat gacggacggt aaaagccggg tatagatacc taacccttgt ttatagctga
     7321 aaatcaataa ggaagaacat tatgtcccaa gtttttaaag attttgattt gtcctccgta
     7381 tgggaatccg acagttgggc agatgaaaac taccaagaag ccccgtttac ccctgaaatt
     7441 ttggctgccg tagaaagtga actgggctat aaattgccgc aaagttttat tgaattgatg
     7501 gcggtacaaa acggcggaat atttgtcaaa aactgttttc cgaccacaca gagaaattcg
     7561 tgggcggaaa atcatgtgca aatttgcgag gtatcgggca tcggctttga aaaagaaggg
     7621 agtttgtgcg gcgaaacagg gcaaaaactt tggctggaag aatgggaata tccgcccatc
     7681 ggcgtgtatt ttgccaacga cccgtcaggc ggtcatgccc tgtttgcctt ggactatcgg
     7741 gcgtgcggca aagacggcga gccgaaagtg gtgtttgtcg agcaagaatc ggattttgaa
     7801 atcgtcgaac ttgcccccga ttttgaaacc tttatccgca gcttgcggca tgaagatgag
     7861 tttattgacg aggaaatata aaacggcggt tgaaaaaccg gaatcatcaa gggaaaacgg
     7921 gcggaataac agataaccga ttgaatccgt aaggaaaacg gttcggcgga acgtgccatc
     7981 caagaccttt gcaaagaaaa actgtccccg acagcattga cattattaac agaacttatc
     8041 aaatttggag ctatcccaaa tataattcgg ttatcctgtt gtatccatta gattatatgc
     8101 ttcaattaaa tgttgttcta gctcttgcac taattgagga ttgcgaattc ctgatacaat
     8161 ctcaaattct tctgcatcta tgcaaacacc tgcataaatt tcaataacaa gggaacgcaa
     8221 taattgaagc tcttctcttg ttaaagaaat aataatgtca tcacctgtgt aattgattat
     8281 attcataata attttatttt tgcttgtcaa agtaagtttt acctaaggtt ggtctaaatg
     8341 cagtcccacc atcttttgaa tttgggtctc taattacaat tgctccagac ttatcatccc
     8401 aaattgctct tatgtgtttg gattgtaatc ttcgaattcc caagaaaaaa atcgtaataa
     8461 gtttgaaagt gtcaaatccc aagtttcttt tgagcaatat tctaatattt tatcaatttc
     8521 acttttaata atcttatgat caaactgttc taatattaat gcattagacc aaaaaaaacc
     8581 ttctttatta caatgatggg aaatccattt aggagaacaa atgcaaagtg aaaaaataga
     8641 tgagccttgt tctccttcga ttccgatatc caaatctatc catctatgga aattatctgg
     8701 aatttcgggg gtaaattttt caaaatcaat atcatataaa tttatgcttt ttaaatccaa
     8761 tttaatcatt agggctgtcc tagataaata gggaaattca aattaagtta gaattatccc
     8821 tatgagaaaa agtcgtctaa gccggtataa acaaaataaa ctcattgagc tatttgtcgc
     8881 aggtgtaact gcaagaacag caacagagcc tgacagcatt gtttatacgg attgttatcg
     8941 tagctatgat gtattagatg tgagcgaatt tagccatttc cgtatcaatc acagcacaca
     9001 ttttgctgaa cgacaaaacc atattaatgg aattgagaac ttttggaacc aggcaaaacg
     9061 tcatttacgc aagtttaacg gcattcccaa agcgcatttt gagctgtatt taaaggagtg
     9121 cgaatggcgt tttaacaaca gtgagataaa agttcaaatt tccattttaa aacaattagt
     9181 aaaatcgagt ttatcttagt tgtccaggac agccccatta tttttataac accgtgaagc
     9241 cgcacagcag tttgaacagt gatacgccgt ttgcgggctt acgagtttat tttcccggcc
     9301 tgcagtttga gcaatacggt gatttcctac ggttaataca aatgtttaca cattgataca
     9361 tttcatttat agttccgcct atttgaaaat agaaaatatg aattcgaccg caagtaaaac
     9421 cctgaaagga ttgtcgctgg tgtttttcgc ctctggattc tgcgccctga tttaccaggt
     9481 cagctggcag aggcttctat tcagtcacat aggtatcgat ttgagttcga ttactgtcat
     9541 tatttctgta tttatggtcg gcttgggtgt aggtgcgtat ttcggtggac gcattgctga
     9601 ccgttttcct tcaagtatca tccccctgtt ttgcatcgct gaagtatcca tcggtctgtt
     9661 cggtttggta agcaggggtc tgatttccgg cttggggcat cttttagttg aggctgattt
     9721 gcccatcatc gctgctgcca atttcctctt attgctgctt cctaccttta tgatgggcgc
     9781 gaccttgccc ttgctgacct gtttttttaa ccggaaaata cataatgttg gcgagtctat
     9841 cggtacctta tattttttca acactttggg tgcggcactc ggatcgcttg ccgccgccga
     9901 atttttctac gtctttttta ccctctccca aaccattgcg ctgacagcct gctttaacct
     9961 tctgattgct gcttcagtat ggctgcgtta cagaaaggat ggatatagtg aacactaaac
    10021 cgaatactag tttgatttat atgctttctt tccttagcgg cttattgagc ttgggtatag
    10081 aagtcttgtg ggtaaggatg ttttcgttcg cagcacagtc cgtgcctcag gcattttcat
    10141 ttactcttgc ctgttttctg accggtatcg ccgtcggcgc gtattttggc aaacggattt
    10201 gccgcagccg ctttgttgat attcccttta tcgggcagtg cttcttgtgg gcgggtattg
    10261 ccgacttttt gattttgggt gctgcgtggt tgttgacggg tttttccggc ttcgtccacc
    10321 acgccggtat cttcattacc ctgtctgccg tcgtcagagg gttgattttc ccgctcgtac
    10381 accatgtggg tacggatggc aacaaatccg gacgacaggt ttccaatgtt tatttcgcca
    10441 acgttgccgg cagtgcattg ggtccggtcc ttatcggctt tgtgatactt gatttcttgt
    10501 ccacccaaca gatttacctg ctcatctgtt tgatttctgc tgctgtccct ttgttttgta
    10561 cactgttcca aaaaagtctc cgactgaatg cagtgtcggt agcagtttcc ctaatgttcg
    10621 gcatcctcat gttcctactg ccggattctg tctttcaaaa tattgctgac cgtccggata
    10681 ggctgattga aaacaaacac ggcattgttg cggtttacca tagagatggt gataaggttg
    10741 tttatggggc gaatgtatac gacggcgcat acaataccga tgtattcaat agtgtcaacg
    10801 gcatcgaacg tgcctatctg ctaccctccc tgaagtctgg catacgccgc attttcgtcg
    10861 ttggattgag tacaggttcg tgggcgcgcg tcttgtctgc cattccggaa atgcagtcga
    10921 tgatcgttgc ggaaatcaat ccggcatacc gtagccttat cgcggatgag ccgcaaatcg
    10981 ccccgctttt gcaggacaaa cgtgttgaaa ttgtattgga tgacggtagg aaatggctgc
    11041 gtcgccatcc tgatgaaaaa ttcgacctga ttttgatgaa tacgacttgg tactggcgtg
    11101 cctattccac caacctgttg agtgcggaat ttttaaaaca ggtgcaaagc caccttaccc
    11161 cggatggtat tgtaatgttt aataccacgc acagcccgca tgcttttgct accgccgtac
    11221 acagtattcc ctatgcatac cgctatgggc atatggtagt cggctcggca accccggtag
    11281 ttttccctaa taaagaactg ctcaagcaac gtctctcccg gttgatttgg ccggaaagcg
    11341 gcaggcacgt atttgacagc agcaccgtgg atgctgcagc acaaaaggtt gtctctcgta
    11401 tgctgattca gatgacggaa ccttcggctg gggcggaagt cattaccgac gataatatga
    11461 ttgtagaata caaatacggc agagggattt aaccgtctta aagggtttca ggcaacgcag
    11521 gttttaggta acgtcctgct agttcaaaaa aaccgcatca cagcagtcgg gacaaaatgg
    11581 tttaaacatt ttgtcccgaa ttcttattcc tatatatagt ggattaacaa aaatcaggac
    11641 aaagcgacga agccgcagac agtacaaata gtacggaacc gatttcagca ccttagagaa
    11701 tcgttctctt tgagctaagg cgaggcaacg ccgtactggt ttttgttaat ccactatacc
    11761 acgaattacg gtgtaaaaat ttatatgacc ttataaaatc aaataagaat cgttatcata
    11821 acatgattgt atttattggg tttttttggg cgttttgccg atatttacct tttaatggtt
    11881 tttgaaattc gctaaaatac gaaattattg tagaaatttt gttaacggat ttgggtgtaa
    11941 ccatgttgtc cgcttacttt cccgtctttg tctttatcct cgtcggcctc gcggccggcg
    12001 tgctgtttat cctgctcggc acgattttag gcccgaaacg ccactacgcc gaaaaagacg
    12061 cgccttacga atgcggtttt gaagcctttg aaaacgccag gatgaagttc gacgtgcgct
    12121 attacctcgt cgccatcctc tttatcttgt ttgatttgga ggtcgcgttc atgttgccgt
    12181 gggcagtcgt gttcaaagat ttgggcgcgt acggcttctg gtctatgctg gtgtttatcg
    12241 tcgttctgac ggtaggcttt gtttacgaat ggaaaaaagg tgcgctggaa tgggaataga
    12301 aggcgttttg aaaaaaggtt tcatcaccac cagcgcggat acggtgttga actatatgcg
    12361 taccggttcg ttgtggccgg ttactttcgg cttggcctgc tgcgccgtgg aaatgatgca
    12421 cgcgggtatg gcgcgttatg accttgaccg tttcggcatc attttccgtc cgtccccacg
    12481 tcaggccgac ctgatgattg tggcgggtac gctgaccaac aaaatggcac ccgccctgcg
    12541 ccgcgtgtat gaccagctcg ccgagccgcg ctgggtattg tctatgggct cgtgtgccaa
    12601 cggcggcggc tattatcact attcttattc cgttgtgcgc ggtgccgacc gcgtcgtgcc
    12661 ggtagacgtt tatgtgccgg gttgtccgcc gactgcggaa gccctgattt acggcctgat
    12721 tcagctccaa caaaaaatca agcgcacttc caccatcgcg cgtgacgagt aaggagagga
    12781 cgatatggca agcattcaaa acttatacga gaccgttgtc ggcgtgcttg gcgatcaggc
    12841 aagcaaagtc atttctgctt tgggcgagat taccgttgag tgtctgcccg aacactatat
    12901 ttcagtcatg accgcattgc gcgaccatga agagttgcat ttcgagcttc tggtcgactt
    12961 gtgcggtgtc gattacagca cttacaaaaa cgaagtatgg cagggcaaac gctttgccgt
    13021 cgtcagtcag ttgctttccg ttaaaaacaa tcaacgcatc cgcgtacgcg tctgggtttc
    13081 agacgacgac ttccccgtag tcgaatccgt agttcccgtt tacaacagcg cggattggta
    13141 cgagcgcgaa gccttcgatt tgtacggcat catgttcaac aaccatcccg acttgcgccg
    13201 catcctgacc gattacggct ttgtcggaca tccgttccgc aaagacttcc cgatttccgg
    13261 ctatgtggaa atgcgttacg acgaagagca aaaacgcgtg atttaccaac ctgttaccat
    13321 tgagccgcgc gagatcacgc cgcgtatcgt ccgtgaggag aactacggtg gccaataaat
    13381 taagaaacta caccatcaac ttcggcccgc aacaccctgc ggcgcacggc gtattgcgta
    13441 tgattttgga gttggaaggc gaaaccatcg tccgtgccga cccgcatatc ggtctcctgc
    13501 atcgcggcac cgaaaaactg gcggaaacca aaacctatct gcaagccctg ccttatatgg
    13561 acaggctgga ctacgtttcc atgatggtca acgagcaggc atactgcttg gcggtagaaa
    13621 aactcgccgg aattgatgta cctatccgcg cccaatacat ccgcgtgatg ttcgccgaag
    13681 taacgcgcat cctcaatcac ttgatgggca tcggttcgca cgcattcgac atcggcgcga
    13741 tgaccgccat cctctacgcc ttccgcgacc gcgaagagct gatggacttg tacgaagccg
    13801 tgtccggcgc gcgtatgcac gccgcctact tccgtcccgg cggcgtttac cgcgacctgc
    13861 ccgacttcat gcccaaatac gagagcagca aattccgcaa tgccaaagta ttgaagcagc
    13921 tcaacgaatc ccgcgaaggc accatgctcg actttatcga tgccttctgc gaacgcttcc
    13981 ccaaaaatat cgacacactc gaaaccctcc tgaccgacaa ccgtatttgg aaacagcgta
    14041 ccgtcggcat cggcgttgtc actcctgagc gcgccatgca aaaaggcttt accggcgtga
    14101 tgttgcgcgg ttcgggtgtg gaatgggacg tgcgtaagaa acagccttac gaaatgtacg
    14161 acaaaatgga tttcgacatc cctgtcggcg tgaacggcga ctgctacgac cgctacctct
    14221 gccgtatgga agaaatgcgt caatccgtac gcatcatcaa acaatgttcc gagtggttgc
    14281 gtgtcaatcc gggtccggtc attaccacaa accacaaatt cgctccgccc aaacgtaccg
    14341 aaatgaaaac aggtatggaa gacctgattc accatttcaa actctttacc gagggtatgc
    14401 acgttcccga gggcgagacc tacaccgctg tcgaacaccc gaaaggcgag ttcggcgttt
    14461 acatcatttc agacggcgcg aacaaaccct accgcctgaa aatccgcgca cccggcttcg
    14521 cccacctgca aggtatggac gaaatggcaa aaggccacat gctggctgat gtggtggcga
    14581 ttatcggcac gcaggatatt gtgtttggcg aagtagacag ataaagctgt aaaaaagcag
    14641 cctgcacttt tcagactgcc tgaaaaaatt attaggaata aaaagcatga atttttggca
    14701 tatgcagatg catccaaccg atgaaatgga gttctctgag aacattgatt ggattttgga
    14761 acacaaaaaa attatcggtt tgggacaatg ggaaggtgga aaaggtcaaa ttgccgcatt
    14821 tatcaatgaa atgcaggtta atgacattgt tgcgctaaaa cgcggtcaag aattgattgc
    14881 gcttgtccaa attaccggtg gggcatataa agttaccgat gatgccaatc tggaaactgc
    14941 atggattgtt aaccgtcgtc ctgttcgcgt attagattgg gcgattgaac cgaaaatatt
    15001 aaaacaagct cgtggtacat taaatcgttg cgtacgtgat gatgtggaaa ctactcaaac
    15061 cattaaacaa tggtatgaag ccgtagtgaa atcatttaca aaacgaagag tggatttaac
    15121 cgtttaaatt tgttttcagg ctgcctgaaa ttccaaaaaa acctaattta ggattacaag
    15181 atgttatcca cagaatcctt aaaacaaatc gacatagagt tggcgaaata tcctgccgac
    15241 caacgccgat ccgccattat gggcgcattg cgtattgccc agaccgaaaa aggctggctt
    15301 gctcccgaga ccatcgcctt tgtcgcagac tacatcggca tatcgcctgc acaagcctac
    15361 gaagtcgcta ccttctacaa tatgtacgat cttgagcctg tcggcaaata caaactgacc
    15421 gtttgtacca acctgccctg cgccctgcgc ggtggtatgg ctaccggcga atacctcaaa
    15481 caaagactcg gtatcggcta cggcgaaatc acgcccgacg gcaaatttac ccttgtcgaa
    15541 ggcgaatgca tgggcgcgtg cggcgatgcc cccgttatgc tggtcaacaa ccacagcatg
    15601 tgcagcttta tgactgaaga ggcgattgag aagaaactgg cggagttgga gtaggtcgtc
    15661 tgaaacgacg atttaaacgt aggtcggata cttgtatccg acagagtggg taaaaaggca
    15721 aaatgtcgga tttaagaatc cgccctactg aaataccgaa atgccgtcat tcccgcgcag
    15781 gcgggaatcc accggtaaga ttcggtttct gaatttaata agacattgct taccattgag
    15841 gatggattcc cgcctgcgcg ggaatgacga cagacaagca agtggtcgag atccaacaaa
    15901 aacgattaaa ggtcgtctga aaatatcgat ttgataaact agattttatt tcagacgacg
    15961 ttacaagccg gtacacaaag acatcttaag gtcgtctgaa acagaggccg caaccgatac
    16021 gaaaacgaat aggcacacca aaatggctat ttaccaatca ggcgtgattt ttgaccaagt
    16081 ggataccgcc aatcccgatt gttggacatt ggacgaatac gtcaaacgtg gcggttatac
    16141 cgccctgcgt aaaatcctgt ccgaaaacat ctcgcaaacc gatgtgattg atgaagtcaa
    16201 aacctccggc ttgcgcgggc gtggcggtgc gggcttcccg accggtttga aatggagctt
    16261 tatgccccgt tccttcccgg gcgaaaaata tgtggtttgc aacaccgacg aaggcgagcc
    16321 gggtacattt aaagaccgcg acatcatcat gttcaatcct catgccctga ttgaaggcat
    16381 gattattgcc ggttacgcga tgggcgcaaa agcgggctac aactatatcc acggcgaaat
    16441 tttcgaaggc tatcaacgct ttgagaccgc tttggcgcag gcgcgtgccg caggcttttt
    16501 gggtaaaaat attttaggct cggattttga atttgaactc ttcgcccacc acggctacgg
    16561 cgcatatatt tgcggcgaag aaaccgcatt gctcgaatcg ctggaaggca aaaaaggcca
    16621 gccgcgcttt aaaccgccat tccctgcttc gttcggcctg tacggcaaac cgactaccat
    16681 caacaatacc gaaacgttct cctccgttcc attcattatc cgtgacggcg gacaggcgtt
    16741 tgccgataaa ggtattccga atgcaggcgg taccaaatta ttctgtattt ccggccatgt
    16801 cgagcgtccg ggcaactatg aagtgccatt gggcacgccg tttgccgaag tcctgaaaat
    16861 ggcaggcggt atgcgcggcg gtaaaaaact caaagccgtt attcccggcg gttcgtccgc
    16921 gcccgtgttg cctgccgata tcatgatgca gaccaatatg gactatgact cgatttccaa
    16981 agcaggctcg atgttgggtt ccggcgcgat tatcgtcatg gacgaagacg tgtgcatggt
    17041 gaaagctctc gaacggttaa gctacttcta ctacgacgag tcttgcggcc aatgtacccc
    17101 ctgccgcgaa ggtaccggct ggctttaccg catcgtccac cgcatcgtag aaggcaaagg
    17161 ctgtatggaa gacttggatt tgctggattc tatcggtaac caaatggcag gccgcaccat
    17221 ctgcgccctt gccgatgccg ctgttttccc tgttcgcagc tttaccaagc atttccgtga
    17281 tgagtttgtg tattacatcg aacacggcgg gccgatgaaa gagcataagt ggggagggtg
    17341 gtaatggcag aaaagaatta ttttggctac ccttgtgatg attgggaagc agctaaacta
    17401 gaagcaaaga aatatttgat tgagaaagtt gcgaaactag gcaatgacat cacttatgga
    17461 aatttggcat tggctattaa aacaattgat attcctgcat acggtgatgc catgagcaat
    17521 ttccttgatg agatgagtcg gtcaacatat gatgcacacg gttttatgtt aagtgctttg
    17581 gtggtctcac agaaaactgg ggaacctggt aaagggtttt ggatattggc aaaagaatta
    17641 aatggagaac agaaaagctg ggggtattgg taccgcaatg agaaaaagaa aatttgggaa
    17701 aaatataaac aatcgtaagg tcgtctgaaa aacacatcgc cgtcattccc gcgtaggcgg
    17761 gaatccatag tggaatttac agaacccgat atttgaaaag cagttgccaa aatccaaaaa
    17821 tggattcccg cttacgcggg aatgacggcg ggagtgggca gatgttttca gatgaaaacg
    17881 gctgtaaatg atattgaaaa aagttgttta tattgcagga aacattaaat gcaaaaccat
    17941 ccgtttgcta gtcaacctgc cgtatatatt ttggcaaacg gtaaaaacgg aacactctat
    18001 atcggcgtta ccatgaattt gccggaaaga gtttggcagc acaaaaacca tgtcaatatt
    18061 gatggcttta ctgcccgata tgatgtgcat gatttggttt ggtatgagtt ttttgagaat
    18121 atgcctgaag cagttgccaa agaaaaaacg atgaaaaaat ggcgacgtga atggaagatt
    18181 aaattgattg aagaacaaaa tcccgaatgg ttggacttgt cgggcgtgtt gtttgtttag
    18241 ttttatttct ggaactttaa aaactgtcgt tattccagcc ccacctacgc gcagacaggc
    18301 tacggcggga atcaccgcaa aagttaagaa accaatgttt gaaaacagtt accgaaaacc
    18361 caagaatgga ttcccgcctg tgcgggaatg acggcaaggt ggcagtaaac gttttaaaca
    18421 gtattgattg tcaatgaaac tcaaaaggcc gtctgaaacc catttttcag acgacctcca
    18481 taaaagatta tttatcaaat acccgtaact aggaacgaac catgttacaa atcgaaatcg
    18541 acggcaaaca ggtatctgtg gagcagggtg cgacggtgat tgaagccgcg cacaagctcg
    18601 gtacttatat cccgcatttc tgttaccaca aaaaactttc catcgccgcc aactgccgta
    18661 tgtgtttggt ggacgtggaa aaagctccca aacctctgcc cgcctgtgcc acgccggtaa
    18721 cggacggcat gattgtgcgt acgcattcgg caaaagcccg agaggcgcaa gaaggtgtga
    18781 tggagttcct gctcatcaac catccgcttg attgtccgac ctgcgaccaa ggcggcgaat
    18841 gccagttgca ggatttggcg gtgggctacg gtaaaaccac cagccgctac accgaagaaa
    18901 aacgttccgt cgtcggcaaa gacatggggc ctttggtttc cgccgaggaa atgagccgct
    18961 gcatccactg tacccgttgc gtccgtttca ctgaagaaat cgccggcttg caggaaattg
    19021 cgatggtgaa tcgcggcgag cattctgaga ttatgccctt tatcggcaaa gcggtggaaa
    19081 ccgaattgtc gggcaacgtc atcgatttgt gtcccgtcgg cgcgctgacc agcaaaccgt
    19141 tccgcttcaa cgcgcgtact tgggaattga atcgccgcaa atccgtttcc gctcatgacg
    19201 ctttgggcag taacctgatt gtacaaacca aagaccatac cgtccgccgc gtgttgccgc
    19261 tggaaaacga agcgattaac gaatgctggc tgtccgaccg cgaccgtttt gcctacgaag
    19321 gcctgtatca cgaaagccgt ctgaaaaacc cgaaaatcaa acagggcggc gagtggatgg
    19381 acgtggattg gaaaaccgca ttggaatatg tccgcagcgc gattgaatgt atcgccaaag
    19441 acggcaacca aaaccaagtc ggcatttggg cgaacccgat gaatacggtt gaagagctgt
    19501 atctggccaa aaaacttgcc gacggcttgg gtgttaaaaa ctttgcaacc cgtttgcgtc
    19561 aacaagacaa acgtctttca gacggcatta agggtgcgca atggttggga caaagcattg
    19621 aatctttggc tgacaacgat gccgtattgg tagtcggcgc gaacttgcgc aaagaacagc
    19681 cgctcctgac cgcccgcctg cgccgcgccg ccaaagaccg tatggcattg agcgtattgg
    19741 ccggcagcaa ggaagaattg tttatgccgc ttctctctca ggaagccgca catcccgacg
    19801 agtgggcagg ccgtctgaaa aacttgtctg ccaatgcgga acacgccgtt accgccagcc
    19861 tgaaaaacgc tgaaaaagca gcggtgattt tgggcgcgga agtgcaaaac catcctgatt
    19921 acgccgcgat ttatgccgcc gttcaagagc tggcagatgc gaccggcgca gtgctgggta
    19981 ttttgccgca agccgccaac agcgttggtg cggatgtctt gggcgtgaac tcgggcgaga
    20041 gcgttgccga aatggcaaac gcgccgaaac aggcagtctt gctgctcaac gtcgagcctg
    20101 aaatcgatac ggttgacggt gcaaaagccg tggctgcgtt gaaacaagcg aaaagcgtga
    20161 tggcgtttac gccgtttgtc agcgaaacgc tgctggacgt gtgcgacgta ttgttgccga
    20221 ttgcgccgtt taccgaaacc tcgggcagct tcatcaatat ggaaggccgt ctgcaatctt
    20281 tccacggcgt ggtacaaggc ttcggcgatt cgcgtccgat gtggaaagtg ttgcgcgtat
    20341 tgggcaacct gtttgacctg aaaggttttg aataccacga taccgtcgcg attctgaaag
    20401 acgcgctgga tgcggaaagc ctgccgtcca aactgaacaa ccgcgccgct tcgactcaaa
    20461 aagattttca gacggcatca agccgcctcg tgcgcgtggg cggagtgggc atctatcaca
    20521 ccgatgctat cgtgcgccgt tccgcaccgt tgcaagaaac cagccatgcc gccgtgcctg
    20581 ctgcgcgtgt aaatccaaat acattggcac gcttgggcct gcaagacgga caaaccgctg
    20641 tcgccaaaca aaacggcgca agcgtatcgg ttgccgtcaa agccgatgcc ggtttgcctg
    20701 aaaacgtggt gcatttgccg ctgcataccg aaaatgccgc gctgggtgcg ttgatggaca
    20761 ctattgaact ggcgggagct tgatcatgca agaatggttc caaaacctct ttgccgcaac
    20821 gctcggtctg ggcgatttgg gtattactgt aggcttggtg gtatccgtca tcgtcaaaat
    20881 tgtgattatc ctgattccgc tgattctgac cgtcgcctac ctgacttatt ttgaacgtaa
    20941 agtcatcggc ttcatgcagc ttcgcgtcgg tccgaacgta accggtccgt ggggtctgat
    21001 tcagccgttt gccgacgtgt tcaaactctt gtttaaagaa gtaacccgtc cgaggctgtc
    21061 aaacaaagcc ctgttctaca ttggtccgat tatgtcgctc gccccgtctt ttgcggcgtg
    21121 ggcggtgatt ccgtttaacg aagaatgggt gctgaccaac atcaatatcg gtcttttgta
    21181 catcctgatg attacctcgc tgtcggttta cggcgtgatc atcgcgggct gggcttccaa
    21241 ctccaaatat tcgtttttgg gcgcaatgcg tgcttccgca caaagcattt cctacgagat
    21301 tgccatgagt gccgcgctgg tgtgtgtcgt gatggtatcg ggcagcatga acttctccga
    21361 catcgttgcc gcacaagcca aaggtattgc cggcggttcg gtattctctt ggaactggct
    21421 gccgctcttc cccatcttta tcgtctatct gatttccgcc gttgccgaaa ccaaccgtgc
    21481 gccgtttgac gtggcagagg gtgagtctga aatcgttgcc ggacaccacg tcgaatactc
    21541 cggcttcgca ttcgcactgt tcttccttgc tgaatacatt ttcatgattc tgatttccgc
    21601 gctgacatcg ttgatgttcc tcggtggttg gttgtctccg ttcccacaaa gctggggctt
    21661 tatcggcacg ccttccgcat tctggatgtt cgtgaaaatg gcggcggttc tgtactggta
    21721 tctgtggatc cgtgcaacct tcccgcgcta ccgttacgac caaatcatgc gtttgggctg
    21781 gaaagtgctg attccgatcg gcttcgccta catcgtggtt ttgggcgtgt ggatgatttc
    21841 accgctgaat ttgtggaaat aagtttcaga cggcatcttg aggccgtctg aacaaagcga
    21901 ttttgaatac ctaacgaaat ccctgttttg agggaacata atatggctaa cttagtaaaa
    21961 acctttctgc ttggcgaatt ggtaaaaggt atgggcgtaa cgctcaaaaa ctttttcgcc
    22021 cgcaaagaca cgatttattt tcctgaagag aaaacgccgc aatccgtgcg tttccgcggc
    22081 ctgcacgcgc agcggcggta tccgaacggt gaggaacgct gcattgcgtg taagttgtgc
    22141 gaggcggtgt gtccggcaat ggcgatcaat atcgaatcgg aagaacgcga agacggcacc
    22201 cgccgcacca agcgttacga catcgacctg accaagtgca tcttctgcgg tttctgcgaa
    22261 gaggcttgcc cgaccgatgc gattgtggaa acgcacattt ttgaatacca cggcgagaaa
    22321 aaaggcgact tgcacatgac caagccgatt cttttggcca ttggcgacaa atacgaagct
    22381 gaaatcgcca aacgcaaagc cgctgacgcg ccgtatcgtt aaggagcaga cgaatgactt
    22441 tttccacgat tctgttctat atccttgccg ccatcgtttt gtacggtgcg gttcgtaccg
    22501 tcaccgctaa aaaccctgtc catgccgctt tgcatctggt gctgaccttc tgcgtgagcg
    22561 cgatgatttg gatgctgatg caggcggagt ttttgggcgt gacgctggtg gtggtttacg
    22621 tcggcgccgt gatggtgttg ttcctgttcg tcgtgatgat gttgaatatc gacattgaag
    22681 aaatgcgcgc cggtttttgg cgtcatgcgc ctgttgccgg tgtggtcggc acattgttgg
    22741 cggttgctct gatcctgatt ctggtcaatc cgaaaaccaa cctggccgca tttggtctga
    22801 tgaaagacat tccggccgat tacaacaata tccgcgattt gggcagccgt atttataccg
    22861 actacctgtt gccgtttgaa ttggcggcgg tattgctgct gttgggtatg gtggccgcga
    22921 ttgcactggt tcaccgcaaa acggttaatc cgaaacgtat ggatcctgca gaccaagtca
    22981 aagtacgcgc cgatcaaggc cgtatgcgtc tggtgaaaat ggaagcggtc aaaccgcaag
    23041 tcgaatctgc cgaagaaagc gaagtttcag acggcctcaa gccggaaggg gagggaaaag
    23101 catgattacc ttgacgcatt atctggtatt gggtgcgctc ctgttcggta tcagcgcaat
    23161 gggtatcttc atgaaccgta aaaacgtgct ggtattgatg atgtccatcg agctgatgct
    23221 tttggcggtg aacttcaact ttatcgcctt ctcacaacat ttgggcgata ctgccggaca
    23281 aattttcgta ttcttcgtat tgaccgttgc cgctgccgaa tctgccatcg gtttggcgat
    23341 tatggtgctg gtgtaccgca accgacaaac gattgatgtt gccgatttgg atgggttgaa
    23401 agggtaaagg taggttgggt cgagacctga caagacaccg atgccgtctg aaaacccgat
    23461 aggaaaaacg atgaaatcca tagacgaaca aagcctgcat aatgcccgcc gcctgtttga
    23521 aagcggcgac atcgaccgta tcgaagtcgg taccaccgcg ggcctgcaac agattcaccg
    23581 ttacctgttc ggcggcttat atgattttgc gggtcaaatc agggaagaca acatttccaa
    23641 aggcggtttt cgttttgcca acgccatgta tttaaaagag gctttggtta aaatcgagca
    23701 gatgcccgag cggacttttg aagaaatcat cgccaaatat gttgaaatga acattgccca
    23761 tccgtttttg gagggtaatg gcagaagtac ccgcatctgg ctggatttgg tgctgaaaaa
    23821 aaacctgaaa aaagtcgtga actggcaaaa tgtaagtaaa accctgtatt tgcaggcgat
    23881 ggaacgcagc cccgtcaacg atttagaact gcgctttctg ttaaaggaca acctgactga
    23941 cgatgtggac aaccgtgaaa tcatctttaa aggtatcgag cagtcgtatt attacgaagg
    24001 gtatgaaaaa ggctgaaggt cgtctgaaaa gcgatttcag attgtttcag acgacctgat
    24061 ttggtaggtg atcagacggg agcggatgag aaaagaaatt ctgggtaaga ataatccggt
    24121 ctgaaatatt ggaagaagaa tgatggataa aaatcagtta gaacaagaat ttcataaagc
    24181 catgttaaat atttatcagg aggctttgaa tttgccgcaa ccttacaagg cgacacgatt
    24241 tttacaaatt gtaaatgaat ttggtggtaa agaggcggcg gataaattat tgagtacggg
    24301 ggaaaagaag actcagaccg gttttacaga gctgattttg agtggtggcg gagtccacgc
    24361 cttgaaatac agtatggaat atctggtgtt acaaaagccg tggtgtgatt tatttactga
    24421 agagcaatta gctgtggcac gcaaacgatt ggagcgtgtt ggatttgttt ttccgaagta
    24481 attttgtacg aaacaaacat agatttttaa atcaatcgga ttcaatcaaa tgaacgacat
    24541 gactttatat ctcatcattg ccctcgtgcc tttggcaggc tcgctgattg cgggtttgtt
    24601 cggcaacaaa attggtcgtg ccggtgcgca tacggttacg atactcggcg tggctgtgtc
    24661 cgccgtgctg tcggcttatg tgctgtgggg ctttatcgac ggcagccgcg ccaagtttga
    24721 cgagaatgtc tatacctggc tgacgatggg cggtttggat ttctccgtcg gcttcttggt
    24781 cgatacgatg acggcgatga tgatggtcgt ggtcaccggc gtatcgctga tggtgcatat
    24841 ctataccatc ggttatatgc acgatgagaa agtcggctac caacgcttct tcagctacat
    24901 ttctttgttt actttcagta tgttgatgct gattatgagc aacaacttca tccagctctt
    24961 cttcggctgg gaagctgtgg gcttggtgtc gtatctcttg atcggtttct atttcaaacg
    25021 tccgagcgca acatttgcca acctgaaagc ctttttgatc aaccgtgtcg gcgacttcgg
    25081 ctttttgctc ggtatcggct tggtgcttgc ttattttggc ggcagcctgc gctatcagga
    25141 cgtattcgct tatctgccca acgtccaaaa tgccaccatc cagctcttcc ccggtgtgga
    25201 atggtctttg attaccgtaa cctgtttgct cttgtttgtg ggtgcgatgg gtaaatcggc
    25261 acaattcccg ctgcacgtct ggctgcctga ttcgatggaa ggcccgaccc cgatttccgc
    25321 attgattcac gctgcgacga tggttacggc gggtctgttt atggtatcgc gtatgtcgcc
    25381 gatttacgaa atgagcagca ctgcgctgtc cgttatcatg gtgatcggtg cgattaccgc
    25441 cctgtttatg ggcttcttgg gcgtgattca aaacgacatc aaacgtgtgg ttgcgtattc
    25501 caccctgtcg caactgggct atatgaccgt ggctttgggc gcgtctgcct attccgtggc
    25561 gatgttccat gtgatgaccc acgccttctt taaagccctg ttgttcttgg cggcaggcag
    25621 cgcgattatc ggtatgcacc acgaccaaga catgcgccat atgggcaatc tgaaaaaata
    25681 tatgccggtt acttggctga ccatgctgat cggtaacttg tcgctgattg gtacgccgtt
    25741 cttctccggc ttctactcca aagattcgat tatcgaagcg gcgaaataca gcacactgcc
    25801 gggcagcggc tttgcctatt ttgccgtcct cgccagcgtg tttgttaccg cgttttacgc
    25861 gttccgccaa tactttatgg tgttccacgg cgaagagaaa tggcgcagcc tgcccgaaca
    25921 ccattcagac ggccacggcg aagaacatca cggtttgggt aaaaacgaca atccgcacga
    25981 aagcccgttg gtggttaccc tgcctttgat tttgcttgcc gttccgtccg tcatcatcgg
    26041 ctacatcgcc atcgaaccca tgctttacgg cgatttcttc aaagacgtga tttttgttaa
    26101 tgccgatgcg catccgacca tgcacattat gaaagaagag ttccacggcg cattggcgat
    26161 ggtgtcgcac agcctgcatt cgcccgttct ctatctcgct atcgcaggcg tattgagcgc
    26221 atggcttttg tacgtcaaac tgccgcacct gcctgcgaaa atcgcgcagg cgttccgtcc
    26281 ggtttacgtt ttgtttgaaa acaaatacta ccttgatgcc ctgtatttca acgttttcgc
    26341 caaaggcaca cgcgcattgg gtaccttctt ctggaaagtc ggcgataccg ccattatcga
    26401 caacggtatt gtcaacggct ctgccagact ggtcggcgcg gttgccgcgc aagtgcgtaa
    26461 agtccaaaca ggctttatct acacctacgc tgccgctatg gtgttcggcg tattggtctt
    26521 gctcggtatg accttctggg gattgttcag ataaaaatga gattttcaga cgacgtttag
    26581 gtcgtctgaa aaaagaaact tgagaaacaa tgtcaaacac acaaaccatc cgctctgccg
    26641 acttcaccac ttcccgcgca tggggcgcac tcgacatcgc caatatgaac ggcaccaccg
    26701 tgcgcctgca ctggacggac cagccctaca aatggcacgt caacgacggc gaggaagtgt
    26761 ttgccgtgat ggatggcgaa gtcgatatgc actaccgaga aaacggcgaa gaacacatcg
    26821 ttcggttgaa aagcggcgac atcttctacg caggaatcgg cgcggaacac gtcgcccacc
    26881 cgcgcggcga aacgcggatt ttagtgattg agaaagaagg cagtgtttag ggcggatacg
    26941 cataagtatt atgtgctttt tcaggtatcc tgaaaatcat aaatctatca acgagacttt
    27001 aatcatggca aatctaaatg tgagtagtaa aaaaattatt aatctgctag gtaatcaaga
    27061 tatgcaggaa aaatatttta tcattcctga atatcaacgc ccctatcgct gggataccga
    27121 aaaatgcgcc gtattatggt ctgatattgt ggattttcgc cagtcgtcca caggaaaaaa
    27181 tgaagaatat tttcttggta caatcgttgt ttgccccaat gaaaaaggta atttggaagt
    27241 aattgatggg caacagcgtt taacatcatt actgctgtta ttgcgtgcgt tttatcgagt
    27301 tttagagcaa acggaaagta cccccgatac cgcagccaaa attaccacac ttaaaagcaa
    27361 aattgccccc tgtatttggg atgttgatgt tctaacggct gaaattaccg atagaaccgc
    27421 aatccggctg catacgcaag ttgccacaga aacagataac gaaattttgc acgagatttt
    27481 ggcaaacggt gaaatctcag aagagtgtcg ttctaattac gccgttaatt atttgtattt
    27541 cctgaaacag tgccaagaat acgccagaac caacccaatg gattggtatc atctttgttt
    27601 aactattttg aatgactgta ttttacttcc gattgaaagt gacagtttaa atagtgcatt
    27661 aaccatattt tccacattaa acgatagggg cttgcctttg tcggatgcag atatttttaa
    27721 agccaggctt tataagatgg cgcagcagaa agataaattt gttgaacaat ggaaggaact
    27781 tgttgcgttg gcagagggtg catctatttc cgtagatgat ttgttccgct attacaccca
    27841 tgtattaagg gcgaaagaaa agattacgga taaagaaatt gggcttcgcc gtttttatac
    27901 tgacaaccga aacaaacagt tggaaaacga aaatttgttc aatcacttaa ttgagctggc
    27961 tggattttgg caggatatta acaacagcaa aactaaccaa gacgtggaaa cttcgaactt
    28021 tgtaaatgac gaggcggcca agtggctgca atgtctgaat gtctatccta atgagttttg
    28081 gaaatacgcc gcgagtgtgt tcttcattgc aaacaagggt gcgcaatcct ttaaagatga
    28141 ttttgcagca ttattaaaac agcttactgc atttttgtat gctaaatttt tagttgcgcc
    28201 aaccgtaact gcaattaaag atgatattta taaattatgt gttgcgattt atcacggtga
    28261 aaaaaacgct ttatcatatg ttatgcctgc acccatacac tatgaacaaa atacaggacg
    28321 tataactaaa gggttgttaa tgattaatgc ttatctattt gatgaaaagc agaaattatt
    28381 gccaactaaa actgaaatcg aacatatctt tccgaaaaaa tggcaggaaa ctaactataa
    28441 tggttggtca atcatagatg cggaacaata tttagaacaa ctaggtaata aaattgtttt
    28501 tgaaaaacgg ttaaatattc aggcaggtaa cggctatttt ggtaagaaaa aggagcaata
    28561 tcaaaaatcc gatattgttg aagtgcgtga attagcaggt gaaaatgcca atgattggtt
    28621 accgagtgat attcagaaac gccagcagca gattgtggat agattgaaac agtttttcga
    28681 taattgtttt gaagcagcca gcatccccaa aaaaaattaa cttttccaat aacctccaaa
    28741 ccaaacaggt aacgcaatgt tttctaacca tcttctcagc ttggcaatat ggatacccat
    28801 tgccgcaggt gtgctggttt tggcgaccgg taaagacagc cgtgcgccgc tggcgcgtgt
    28861 gcttgccttt atgggtgcgc ttgccggttt cttggtaacg ctgcccctgt ttaccggttt
    28921 cgaccgtttg agcggcggct atcaatttac cgagttccac gagtggattc cgctgctgaa
    28981 aatcaactat gcattgggcg tggacggtat ttcggtgctc tttatcatct tgaatgcgtt
    29041 tattacgctg ttggtggtat tggccggttg ggaagttatt cagaaacgcc cggcgcagta
    29101 tatggcggca ttcctgatga tgtcgggttt gattaacggt gcgtttgccg cgcaggatgc
    29161 gattctgttt tatgtgttct tcgagggtat gctgattccg ctgtacctga ttatcggtgt
    29221 atggggcggc ccgcgccgcg tctatgcgtc ggtcaagctg ttcctctata cgctgatggg
    29281 ttcgctcctg atgctggttg ctatggttta cctgtactat caaacaggca gcttctctat
    29341 tgtcgatttc caaaacatca aacagattcc gttgggcgta cagcagcttt tgtttgcggc
    29401 attcttcctg tcatttgccg taaaagtgcc gatgttcccc gtacacactt ggctgccgga
    29461 tgcccacgtt gaagcgccga ccggcggttc gatggtgttg gcggcgatta cgctgaaact
    29521 gggtgcgtat ggtttcttgc gctttatcct gccgattatg ccggacgcgg cacgctattt
    29581 tgcccccgtg atcatcgtat tgagcctgat tgccgtaatt tatatcggta tggtggcttt
    29641 ggtgcaaacc gatatgaaaa aactggtggc gtattcgtcc atcagccata tgggttttgt
    29701 aacgcttggg atgtttttgt ttgttgacgg gcagttggac gactgggcat tgaaaggtgc
    29761 aatcattcaa atgatttccc acggtttcgt gtctgccgcg atgtttatgt gtatcggcgt
    29821 gatgtacgac cgcctgcata cgcgcaatat tgccgattat ggcggcgtgg tcaatgtgat
    29881 gcccaagttt gcggcgttta tgatgctgtt cggtatggcg aacgccggtc tgcctgcgac
    29941 ttccggcttt gtgggcgagt ttatggtgat tatgggcgcg gttaaagtga atttctgggt
    30001 aggcgctttg gccgccatga ccctgattta cggtgcgtct tataccctgt ggatgtacaa
    30061 gcgcgtgatt ttcggcgcga ttcacaatcc gcacgttgcc gaaatgaaag acatcaactg
    30121 ccgcgaattt gcgattctgg cggtgttggc ggttgctgtt ttgggtatgg gcctgtatcc
    30181 gaacgcattt atcgaagtgg tgcatcaggc ggcaaacgat ttgattgccc atgtggcaca
    30241 aagcaagatg tgaggtgtgt aaatgaactg gtctgatttg aatttaatgc ccgccatgcc
    30301 cgaaatcgtg ctgcttgcgc tgctggtgtt attgttgctg gcggacttgt gggtcagtga
    30361 tgacaaacgc cgttggacgc attacggcgc gttggcaacg gtggcggtta cggctgtggt
    30421 gcagcttgcc gtttgggaac agggcagcac gtcttcgttc aacggcatgt atattgcaga
    30481 cggtatgtcg cgtttggcaa aaatggtttt atatgccttg acgtttgccc tgtttgttta
    30541 tgccaagccc tacaaccaag tgcgcggtat tttcaaaggc gagttttaca ctctgtcgct
    30601 gtttgccctc ttgggcatga gcgtgatggt gagcgcgggg cattttttaa ctgcctatat
    30661 cggtttggaa ctcttgtcgc tcgcccttta cgccctgatt gccctgcgcc gcgattccgg
    30721 ctttgccgcc gaagccgcct tgaaatattt tgttctgggc gcgctggcat ccggcctgct
    30781 gctctacggt atttctatgg tttacggcgc aaccggttcg ctggaatttg ccggcgtgct
    30841 cgcctcttcc ttcaatgaag aagcaaacga atggctgttg aaactgggtc tggtgtttat
    30901 cgtcgtcgcc gttgcgttca aactcggcgc ggtgccgttc catatgtggg tgcccgacgt
    30961 gtatcacggc gcgcccactt ctgttaccgc cctagtcggc actgccccga aaatcgccgc
    31021 cgtcgttttc gctttccgca tcctcgttac cgggttggga accgtgcatc atgactggtc
    31081 tctgatgttt gccctgcttg ccgccgcctc gctgctggtc ggcaaccttg ccgccatcat
    31141 gcagaccaat atcaaacgta tgctcgccta ttccaccgta tcgcatatgg ggtttatcct
    31201 gctggcgttt atggcgggcg cggtcggctt tgcggcgggg ctgtattacg ccattaccta
    31261 cgcgctgatg gcggcggcag ggttcggcgt gttgatggtg ttgtcggacg gggacaacga
    31321 gtgcgaaaac atcagcgatt tggcagggtt gaaccaacac cgcgtatggc ttgccttttt
    31381 gatgctgctg gttatgttct ccatggcagg cattccgccg ctgatgggtt tttacgccaa
    31441 attcggcgtg attatggcac tcttgaaaca aggctatgtt tggttgtccg tatttgccgt
    31501 catcatgtcc ctgattggcg cgttctacta cctgcgcgtg gtcaaagtca tgtattttga
    31561 cgaatccggc cgcgcccatc ccaccgtcgg cggcaacaat gccgcaaaat tccttttgag
    31621 cgtcaacgcg ctgctgttgg tgctgtgggg cattatgccg caaaccgtta tcgactggtg
    31681 cgccaaggcg ttggaaaaca cgctgtaagc cgccgcaacg gcagccgtgt cagaggctgc
    31741 cgtttttgtt aagatatgcc gttccgcaac gcagttcaga cggcatcgcc gccgacaatg
    31801 cccaaacaga aagcccgcca tgaccgcatc catgtacatc cttttgctgc ttgccttgat
    31861 ttttgccaac gcccccttcc tcacgaccaa gctgttcggc atcgtaccgc tcaagcgcaa
    31921 acatttcgga caccacctga tcgagctggc ggcaggtttc gcgctgaccg ccgttcttgc
    31981 ctacatcctc gaatcccgtg cgggagcggt acacgatcag ggttgggagt tttacgccac
    32041 cgtcgtctgc ctgtacctga tttttgcgtt tccctgtttc gtgtggcggt atttttggca
    32101 cacgcgcaac agggaatagg caggcatagg aatgccgtct gaaacctgtt cagacggcat
    32161 ttgtttcatt caagtgcagg ccggcatcgc tgtgccggca cgtttcagcc ggcgatatac
    32221 gccggtttta atatttgcgg gcgactgcaa attctgccaa ctgccgcagg cgcagggctt
    32281 tgtcgccgaa gggttcgagc agcgcgaccg cttcggcaac cagtttgtgt gcgtatgagc
    32341 gcgccgcttc caagcccatc agtttcacat aagtcggctt gtcgttgtct gcgtctttgc
    32401 ccgccgtttt gcccaaagtc gccgtgtccg cttcacaatc caacacatcg tcaatgactt
    32461 ggaacgccag ccccagtttt gccgcgtaag cgtccaatac ggaaagttcc gcatctgaca
    32521 gatcaggaca cgccgtcgcc cccaataaaa ccgccgcacg gattagcgca cccgttttca
    32581 ggctgtgcat ctgttccaaa tcggcttgaa ccatttgttt gccgacattc gccaaatcga
    32641 ttgcctgacc gcccgccatg cccgcgctgc cgcccgcttt cgccaacacc gacaacattg
    32701 ccaactggcg tgctgcgggc agttctgtcg gacggcttaa cacgtcgaag gcttgggttt
    32761 gcaaagcgtc gccgaccagt agcgcggtgg cttcgtcgta ttgcacatgg caagtcggtt
    32821 tgccgcgccg caggctgtcg ttgtccatcg ccggcatatc gtcgtgaacc aaagaataga
    32881 cgtggatcat ttcgattgcc gccattgcct gttctactgc ttcatgcacg accccgccca
    32941 attccgaagc cgccagcacc agcatcggac gcagacgctt gccgccgtcc aaagccgcat
    33001 agcgcatcgc ttcgtgcagc gtgtgcggga tttcgttttc agacggcaaa aagcgttcca
    33061 gcagcagctc tgtctgtgcc tgcgccctct gttgccacgc tttcaaatca ttcgtcggat
    33121 tcaaggttca actccttcag cccgtccgca tccaaaacct gtaatttctg ttcgacttgc
    33181 gccagcttgg tttggcagta tctgaccagc tcgttgcctt cctgataggc ggcaagcgcg
    33241 tcttccaagg gcatttcgcc ctgcatagct tgtgtcagcg attcaaggcg cgacaaggct
    33301 tcttcaaacg atttcggggc gtttttcttc atcgtgtttc cttttcggtt gaaaccccgc
    33361 cacttggaca tctgtccttc ggggcggtag aatcagactt tatttgggag gggtgtaagc
    33421 ccttccaaat caggacgaca catagggcgg tgctttaagt gtcgtcctgc gtgttggaac
    33481 atagtttcgg atgttccggt aaaaagcgga ttgtagcatt tttgaaaaac ggatgccgtc
    33541 tgaaacccga atccggtttc agacggcatt gttttgtcct gcaaacggca aggcgttacc
    33601 cgggcagttc gtcggtgatg ccctgcaaaa aggcgaggcg ttcggggctt gccgccctgg
    33661 tttcggcggc ggctttgaag gcgcagccgg gttcggcgcg gtgggtgcag ttgtggaagc
    33721 ggcattgccc gacaaggtgg cggaaatcgg ggaaatagcg cggcaaatcg gcggcttgga
    33781 ggtggtgtaa accaaattct tgcaaacccg gggagtcgat gagttgggtt tcgccgttca
    33841 aatcgtaaag ccgggcatgg gtggtggtgt gttttcccga gtcgagtgcg gcggaaatgt
    33901 cgccggtgcg ggcggtttgg ctgcctaaaa gggcgttggt cagggtggat ttgcccatac
    33961 cgctttgtcc gagcaggatg ttgctgtgcc cttgcagggc ggggcgcagg ctgccggcgt
    34021 tttccagtgc gcgggtttcg atgacgggat aacccagcgt ttcgtagaat ttgagttttt
    34081 cgcgccaaag ggcggtttcg ggcaggtcgg ctttgttcag gacgatgacg gcgcggatgc
    34141 cggcggcttc ggcggcaagc agggcgcgct gtagcagccg cacgctcgga ctcgggacgg
    34201 cggcggttac gatgaggagt tgggtaacgt tggcggcaat gagtttggtt ttccacgcgt
    34261 cttggcggta gagcaggctt tggcgcggta aaaaatcttc aatcacaact tgttcggcgt
    34321 tgacggggct gatgcggacg cggtcaccgc aggcgaaatc gacgcgtttt ttgcgggtgc
    34381 tggcttcgta ggttgtgccg tcgggcgtgc gcacgatgta gcggcggccg tagctggcgg
    34441 taatttgggc ggtgtcgttc atggtttctt tggggttggg tgtggaaatg ccgtctgaaa
    34501 acgggtgttc ggacggcatc ggttcagtcg tgctgccact cgacgtgttc gttgaggaag
    34561 ccgccgctct ggtgcgccca gagtttggcg taaagcccgc gtttttcgag gagttcggcg
    34621 tgtgtgcctt cttcgatgat gcggcctttg tcgaggacga cgagcctgtc cattgcggcg
    34681 atggtggaga ggcggtgggc gatggcgatg acggttttgc cgtccatcat tttgtcgagg
    34741 ctttcctgga tggcggcttc gacttcggaa tcgagcgcgc tggtggcttc gtccaaaaga
    34801 agaatcggcg cgtctttgag catcacgcgg gcaatggcga tgcgctggcg ttgcccgccg
    34861 gagagtttca cgccgcgttc gccgacgtgc gcgtcatagc cgcgccgccc tttggcatcg
    34921 gaaagttcgg ggatgaagcc ggcggcttcg gcgcgttcgg cggcggaaac catttcggca
    34981 tcggtcgcgt cggggcggcc gtaaataatg ttgtcgcgca cggaacggtg cagcagcgag
    35041 gtatcttgcg tgaccaaacc gatttgggcg cgtaaagatt cttgggtgat gccgcttatg
    35101 tcctgcccgt cgatcgaaac cgtgccgctt tgcggttcgt agaagcgcaa aagcaggttg
    35161 acgatggtgg atttgcccgc gccgctgcgt ccgatcaagc cgaccttttc gccgggtcgg
    35221 atggtgaggt tgaagccgtt gagcagcggt ttgcccgctt cgtaggagaa atcgacgtgt
    35281 tcaaatttga ttgcgccttg cggcactttc aacggcaggg cgttcggctt gtcgaggatg
    35341 gtgtgcggtt tggacagggt tgccatgccg tcgccgacgg tgccgatgtt ttcaaacagc
    35401 cgcgcggatt cccacataat gtattgcgac aaaccgttga cgcgcaacgc catggcggtg
    35461 gctgtagcaa ccgcgcccac gccgacctgc ccgttgtgcc agagccagat gcccagtgcg
    35521 gcggtggaga gggtcaggga ggtgttgacg atgaagctgc acgaatgcag cagcgtcgcc
    35581 agccgcattt gggcgcgcac cgtaaccata aattcttcca tcgactgctt ggcataggcg
    35641 gcttcacgcg cgccgtggga gaagagtttg acggtggcga tattggaata ggcatcggta
    35701 atgcggccgg tcatcagcga gcgggcatcc gcctgccatg cggcggtttg ccccaatttg
    35761 ggaatcagca ggcgcatcac cgaagcgaaa ccgacaatcc agccgataaa gggcagcagc
    35821 agccatgagt cgagcgaggc gagaatcacg ccggaggtaa tgaaatacac cgacacataa
    35881 acgaccatat cggcaaccgt catcaccgcg tcgcgcaacg ccagcgcggt ctgcatgact
    35941 ttggcggaca cgcgtccggc aaattcgtcc tgataaaaac cgaggctttg gttcagcatc
    36001 aggcggtgga agttccagcg caggcgcata gggaacacgc cctgaagggt ttgcaggcgc
    36061 acgttggacg cggcaaacgc ccacgcaacc gaaaacacca tcatcgccgc cattgccgcc
    36121 agttcccaac ctttttcggc aaacagttcg gcgggcgcgt atttgccgag ccactccacg
    36181 attttgccca taaattgaaa aaccaaggct tccataatgc cgatgccggc ggtcagcgca
    36241 gccagggcgg ctatccattt ccgcacgccg gtcatgctgc tccagacaaa ccgccacaag
    36301 cctttttctg gcgttttcgg ggcggcttcg ggataagggt cgattcggga ctcgaaccag
    36361 gaaaatattt tgttcaacat tgttttcgat ttcggtaaaa cagtttcaga cggcatcaaa
    36421 cacaatgccg tctgaaagga aggacaataa cgccatttta cgggaaaagc cgtcgggaag
    36481 acagcgcgag gcggaaacgc agggtttcgt cagggcaaac gtcgcgccgc cttcagacgg
    36541 cattatttca gcaggttttt caaagcaagg cgcacgcctt cgcccacgtc cgtccctttc
    36601 ggaacgcctt tgaccgccgc ttttgcctcg cgttcgctgt aaccgagggc gaggagcgtg
    36661 ccgacgatgt cttccgtttc gtcggcggcg ggtgcggcgg caaacagccc gtccgttacc
    36721 gcatgcgcga ccagcttgcc gcgcagttcc aaaaccatgc gttcggcggt ttttttgccg
    36781 attcccgggg cggaggagag gcgtttgaca tcttcttctg caaccgcctg cgccagctcg
    36841 tctgccgtca tcgccgacaa aatgcccaaa gccgttttcg cgccgatgcc gctgacctta
    36901 atcagttggc ggaatgtttt gcgttcttcc gcagtggcaa aaccaaataa aagatgtgcg
    36961 tcttcccgaa tgataagctg ggtaaacagt tgtacgcttt cacccacggg cggcaggttg
    37021 tagaaggtct gcatcgatac gtcggcctca tagccgacac cgttgacatc gatgacgatt
    37081 tgcggagggt ttttttcaac cagtttgccg gtcagtctgc tgatcatgta tgccgaatcc
    37141 tgaagtgtcg ggtgcaaaat gccgtctgaa accggttcgg gcttcagacg gcacggattg
    37201 tatcaaattc agtcgtcgcg gcgggaggaa atcacgcggc cggtacgggc atcgacaacg
    37261 actttgtatt cctgtccgtt tttgacgatt tcgacatcat agtgcggacg gccgttgtcg
    37321 tgttcgagat cgatgtcggt gattttgccg ccgacacgcg ccaacgctgc tttttcggct
    37381 tgggcgcggc tgatgatttt gtcttgtttg ctgtgttggt gtgcggcgtg tccgtggtcg
    37441 tcatcgccgt gtccgtcgtg gtgggcgagt gcgggggcgg aaatgcccat cagtgcggtt
    37501 gcggcggcgg tcaagagaag gcgtttgaag ttcatgtttt gctcctgcga agtatctggt
    37561 ggtgtttgaa agacgtaaag gcgggacatc aaccggcggt caataccgtc tgaatcatac
    37621 cgcgcgcctg agcttcggcc tcggcggcgc gttcctgcga ggcaaacgga cccattttga
    37681 cgacgtattc gtaacggcgt ttttcaatgg cgaggttcgg gcttgatgtc gaagcggcga
    37741 agttttgggc ggcttggttc agataggctt gtgcttcgtg ttccgtaccg aaagatttca
    37801 agtcgataaa gatgtctttg ttttcggcaa ccggtgcgga tttgcccggg acgatttgtt
    37861 cgattttgac gtgcgccgtc ccttggctga caaagcccaa tttttgcgcg gcggctttgg
    37921 atacgtcgat gatgcggttg ccgtggaagg ggccgcggtc gttgacgcgg acgatgacgc
    37981 ttttgccgtt tttggtattg gttacgcgca catagctggg gatgggcagg gttttgtggg
    38041 cggcggtaaa ggcgttcata tcgtatcgtt ctccgccgga agttttgcgc ccgtgaaacc
    38101 tgccgccgta ccacgaggcg ttgccggttt gcgtgaattc ggcgacttgg tttttcggcg
    38161 tgtagcgttt tccggcgact ttgtagctgc ggttggcgga ggcgtgcagt ttttctgcct
    38221 tgaccactgc gtcggcggat gccgtctgaa gggagtgtgt gccgaatgcg gcggtgagaa
    38281 ggaaaagggt ttttcgggtt aaagtcaaaa cgtgttccgt tcttgagttg aagacgaatg
    38341 ggcatcatgc ccgccggata cgttccgaac cgccgtacag tgcggacggc ggttcggaat
    38401 gtgtccggat aggttttcag acggcatgaa cttgcgttca aacgccgcct gcgtaaccgt
    38461 gttgccgcca cgcttcaaag agaatcacgg caacggtatt ggaaaggttc atactccggc
    38521 tgccgggctg catcggcagg cggatttttt gcgcggcggg caggctgtcg aggatgtcgg
    38581 caggcagtcc gcgcgtttcc ggcccgaaca gtaaaacgtc gcctttttga aacgcggttt
    38641 catcggggcg cgccgtgcct ttggtggtca gggcgaaaat gcgcctgcct gcgagtgcct
    38701 tgaggcagtc gtcgaagttt tcgtacaccg tcaggctggc gaattcgtgg tagtcgagcc
    38761 cggcgcgttt cattttggcg gaatccaatg ggaagccgag cggtttgaca aggtgcaaat
    38821 ccgcgccggt attggcgcac aggcggatga tgttgcccgt gttcgggggg atttccggtt
    38881 ggtataaaac gatggtaaac ataaatatca atcacttata ggcgcgtaac cttgccacaa
    38941 ggcggatggg gtgtcaaaaa atttagttat tttttcattg gcgtgcgtgc cagcgtccag
    39001 cagcagatgc ggtttgcgcc taattttttc agcgtctttg ccaattcgtc cagcgtcgcg
    39061 ccggtggtaa agacatcgtc gattaacaga atattacagt tttccggtat cggtgtgcgg
    39121 atttcaaagg cgtttttgat gtttcgccgc cgttcgccgc ctttgagcgt gctttgcggc
    39181 gggcggtggt gtcggaaaac ggtgtgtcgg ggcagtatct gccagccgta gcgttgtgcc
    39241 agcagcccga cgatgctttc actttggttg aacccgcgtt gcagcagccg ctccctgctt
    39301 agcggtacgg gcaggacgaa atcgaaacat tcgtctgcaa gccggtcggg cggattctgc
    39361 atcatcaggt ctgccagcgg ctgcaccatg ctcaaatcag ccaagtgctt cagcgcgtgt
    39421 atcatattgc tgacgggcgg ttcgtaatgc agcgaggccc acatccggtc gaatgcgggc
    39481 ggtttccttt ggcaaatgcc gcacaccgcg ccgccttgga tgtgtctgaa acacaggggg
    39541 cagctgtttg ccgcgtcggt gcggtatgcc gccaaatcgt tgcggcagcc ggcgcagatg
    39601 ccgtctgaaa cgccggacga accgtggcat aatacgcaac gcctgatagt gggcgcgtct
    39661 gcgatgcgcc gccaacaaga gagaaaatcc atgcctgatg ccgtcaaaaa agtttacctg
    39721 atacacggtt ggggggcgaa ccgccacatg ttcgacgatt tgatgccgcg cctgcctgca
    39781 acgtggccgg tgtccgccgt cgatttgccc ggacacggcg acgcgccctt cgcgcaaccc
    39841 ttcgatattg aggcagccgc cgacgctgtt gccgcacaaa tcgatacgcc cgccgacatt
    39901 ctcggctggt cgctcggcgg attggtcgcg ctgtatctgg cggcgcgcca tcccgacaaa
    39961 gtccgttcgc tctgcctgac ggcgagtttc gcccgactga cggctgacga agactatccc
    40021 gaagggcttg ccgcgcctgc attgggcaaa atggtcggcg cgttccgttc ggattatgcc
    40081 aaacatatca aacagtttct acaactccag cttctgcaca cgcctgatgc ggacggaatc
    40141 ataggcagaa tcctgcccga tttggcgcgc tgcggcacgc cctccgcctt acaggaagct
    40201 ttggacgcgg cggaaagggc ggatgcgcgg catttgttgg acaagataga tgttccggta
    40261 ctgctggtgt tcggcggcaa agacgcgatt acgccgccgc gtatgggcga gtatctgcac
    40321 cggcatttga aaggcagcag gctggttgtg atggaaaagg cggcacacgc gccgtttttg
    40381 agccatgcgg aagcgtttgc cgcgctgtac cgcgactttg tcgaaggggt ttgacatgaa
    40441 tccgcaggac aaatgctggc aggttcaccg ccatctcgcc gaatatgccg accaacgcct
    40501 gacactcgtc cgcaacgcgc ccaagtatat cctgcttgcc ggtgcggatg cggacatcag
    40561 ccgcagcctg ctggcgaaac gctatccgca ggcggtattt gaagaatacg attcccgtgc
    40621 ggactttttg gcggctgccg ctgccgcccg caaaggcggt ttttggcaaa ggtttacggg
    40681 taagggcgtg gtgcaacact gccaatcccc gatcgcgccg ctgcccgaag cgtgtgccga
    40741 tatgttgtgg tcgaatctcg gactgttggc ggcggaacaa atccttcctg tgctgcacaa
    40801 ctgggcgtgc gccttgaaga cggacgggct gctgtttttt acctgcttcg ggcgcgacac
    40861 ttttaccgaa ctgaaatgcc gtctgaaaga aaacggcatt gaaagccgca gcgtgatgtt
    40921 tcccgatatg cacgacttgg gcgatatgct tgctgaaaac ggcttttacg accccgttac
    40981 cgatacggcg aagctggtgt tggattacaa aaaggcggaa acgttttggg cggatatgga
    41041 cacgctgggc atctggcggg cgatggcgtg gaacgatgaa aacgccgcgc gttcgtgtgt
    41101 cgggacaata tttgagcggg aaggcggttt gggcattacg ctggaaacgg tgtacggaca
    41161 cgccgtgaaa aaactgatgc tgccgcaggg ggagaacgtg gtgcagtttt ttccgaagag
    41221 atgatgtgca gatgccgtct gaagccgtgt ccaagtttca gacggcatct gtctgtgaaa
    41281 accgatagaa ataaaggaaa taccgatgta tagtgaatta aatttaaacc agtacggcgt
    41341 tgcctcgcct tagctcaaag agaacgattc tctaaggtgc tgaagcacca agtgaatcgg
    41401 ttccgtacta tttgtactgt ctgcggcttc gccgccttgt cctgattttt gttaatccac
    41461 tatatgctga tgccggagac gtatattgcg tctataacat cagactgaag cagtacactg
    41521 cctgccaggt tacccgagtt gaagaacacg gtggcaaaaa aacacatgcg accctgctgg
    41581 ctttggactg gcagggcaac aaaccgcttg gggcggcgga actggcggat ttgaaaccgc
    41641 tctacaaaga ctttatgtat tgggaacgcg gtctgcacat gtataaggca agtgccgtcg
    41701 tgccgacggg atatgtacgg gttgggaata ccgcgccgct ggtcggcgaa gacacgcagc
    41761 agtatgcctc tttttggggc gacggctacg acgtgtaccg tcagttgaga tggcagcaga
    41821 tacccgaaaa acagagaaag gcattcaaaa aagccgccaa aagcaaaaag accgtgatgt
    41881 ttgccggacg ggaatacggc atatccaaac agaatttgag cgatgtttgg gacgattttg
    41941 aagacgcgat ggaactgaag gcgtttccct gcctgtcttc gctgtttctg accaaatggc
    42001 ataaaaatct atatgatagt ggattaacaa aaaccagtac
//
LOCUS       DAWFHY010000006        41728 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.006, whole genome shotgun sequence.
ACCESSION   DAWFHY010000006 DAWFHY010000000
VERSION     DAWFHY010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 41728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.006"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(106..2577)
                     /locus_tag="WGQ68_000262"
     CDS             complement(106..2577)
                     /locus_tag="WGQ68_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HEZ0714145.1"
                     /translation="MKPLQMLPIAALVGSIFGNPVLAADEAAIETTPVKAEIKAVRVK
                     GKHNASTTVERVKAKTIQEQMIRDTRDLVRYSSDVGVSDDGRRMKGFAMRGVEDNRVG
                     ISIDGVALPSSEENSLYARYGNFNNSRLSIDTELVKGVDITKGADSFESGSGSLGGNV
                     NYRTLDARDIVLPEQNFGALLRSGYASKNREWANTAGFGYAGNVFDAVVLYSRRRGHE
                     MKSGGDVVDFVGFGELDKQDQAERGSARIHPDPSKHNNHSYLVKLGWQPVDAHRFGFS
                     VNGQNNSNYTYEKTYSLTSYWREADDVQKRSNINLNHEWTPDSSILSLLRTDLDYQKT
                     RNGAINYKGDMVRTGDWRTGYQYHNGFMADRDERNMDTKYKRITFRLDSQPFSLWKGE
                     HRLSFKTFASRRDFENVNDDLDFNSTGNITGRKHYTIMRPMKTDQIGFSLQDKIAFGD
                     KLSVNAGVRYDHEKVKPQAFKPDTPCLSTCVNDGNPSARSFNTWNGVLGLDYLFNDTW
                     RAAYQINTGHRVPTASEMYFTYTSPYGNWIANPSLKAERSLNQTLSLRGKNDKGLLDF
                     SLYQTRYRNFLFEEEKTYQVPNEYYNEHSCYYNPKYCNPTLTEIGQQMVNIDKARVRG
                     VEFKGELNLHQVMSVPEGFKVSGALGYSKGKLSGERGSLLSIQPLKLVLGFDYESPDE
                     RWGIFSRVSYMGRKKAKDAQVIEQKSYCTSYKLDPWTGKETDDCAVALQYRDDKIDFR
                     WLNKAATVVDVFGYYKPVKRMTLRAGVYNLFNRKYHTWDSLRGINPRSTINSLSVSNT
                     KSANQGLERYYAPGRNYAVSLEWKF"
     gene            complement(2752..3381)
                     /locus_tag="WGQ68_000263"
     CDS             complement(2752..3381)
                     /locus_tag="WGQ68_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biliverdin-producing heme oxygenase"
                     /protein_id="HEZ0714146.1"
                     /translation="MSETENQALTFAKRLKADTTAVHDSVDNLVMSVQPFVSKENYIK
                     FLKLQSVFHKAVDHIYKDAELNKAIPELEYMARYDAVTQDLADLGEEPYKFDKELPHE
                     TGNKAIGWLYCAEGSNLGAAFLFKHAQKLDYNGEHGARHLAPHPDGRGKHWRAFVEHL
                     NALNLTPEAEAEAIQGAREAFAFYKVVLRETFGLAADAEAPEGMMPHRH"
     gene            3817..5190
                     /locus_tag="WGQ68_000264"
     CDS             3817..5190
                     /locus_tag="WGQ68_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="paraquat-inducible protein A"
                     /protein_id="HEZ0714147.1"
                     /translation="MHPDWVYRLFSFIMPPLIFATFPMKPFAENIPHSLRGNCRDEAL
                     PPHTVDCPECGCRADVPRLDRGEAAFCPRCGYKLFRVGRHPFSAPPAYAAASLILMAF
                     AYGMTYIEVGIPGAASVLSLPEMMRLMVFQDYGFLAEVMFVLTFGAPVLFLLLCLYVY
                     AALIRKQAYPALRLATRVMVRLRQAMMVDVFFVSTLVAYIKLSSVAEVRFGSAFYLMF
                     ALSVMLIRTSVSVPQHWVYFQIGRLTGDNAVQTASEGKTCCSRCLYFRDSAESPCGVC
                     GAELYRRRPKSLSISSAFLTAAVILYFPANILPIMISSNPAATEVNTILNGIAYMWDE
                     GDRLIAAVIFSASILVPVLKIAAMSVLIASARFALPTGAKKLSHLYRITEAVGRWSMI
                     DIFVIIILMCSFHTYAARVIPGSAAVYFCLVVILTMLSAYYFDPRLLWDKRASDGIAF
                     NETEKHD"
     gene            5183..6844
                     /gene="pqiB"
                     /locus_tag="WGQ68_000265"
     CDS             5183..6844
                     /gene="pqiB"
                     /locus_tag="WGQ68_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HEZ0714148.1"
                     /translation="MTDNSPPPNGHAQARVRKNNTLLSAVWLVPLIALIAGGWLWIKE
                     IRNRGPVVTLLMDSAEGIEVNNTVIKVLSIDVGRVTRIKLRDDQKGVEVTAQLNADVS
                     GLIRSDTQFWVVKPRIDQSGVTGLGTLLSGSYIAFTPGKSDEAKDVFQVQDIPPVTAI
                     GQSGLRLNLIGKNDRILNVNSPVLYENFMVGQVESAHFDPSDQSVHYTIFIQSPNDKL
                     IHSASRFWLESGINIETTGSGIKLNSAPLPALLSGAISFDSPKTKNSKNVKSEDSFTL
                     YDSRSEVANLPDDRSLYYTAFFKQSVRGLTVGSPVEYKGLNVGVVSDVPYFDRNDSLH
                     LFENGWIPVRIRIEPSRLEINADEQSKEHWKQQFQTALNKGLTATISSNNLLTGSKMI
                     ELNDQPSSSPKLRPHTVYAGDTVIATQGGGLDDLQVKLADLLDKFDKLPLDKTVAELN
                     GSLAELKSTLKSANAALSSIDKLVGKPQTQNIPNELNQTLKELRTTLQGVSPQSPIYG
                     DVQNTLQSLDKTLKDVQPVINTLKEKPNALIFNSSSKDPIPKGSR"
     gene            6844..7362
                     /locus_tag="WGQ68_000266"
     CDS             6844..7362
                     /locus_tag="WGQ68_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HEZ0714149.1"
                     /translation="MRLFPIAAALSLAACGTVQSTQYFVLPDSRYIRPATQGGETAVE
                     VRLAEPLKRGGLVYQTDPYRLNTAQNHVWADTLDDMLEAALSNAFNRLDSTRIFVPAS
                     RSGSTEKWTVYIDAFQGSYTGKTLISGYAVLPDGTNRPFHIETEQQGDGYAAMTAALE
                     QGLKQAAQQMVE"
     gene            7366..7917
                     /locus_tag="WGQ68_000267"
     CDS             7366..7917
                     /locus_tag="WGQ68_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEZ0714150.1"
                     /translation="MNYCEFAASLPENTDNPNKHYHDTQYGFPIDSDNGLFERLVLEI
                     NQAGLNWTLMLKKQQAFQTAFKGFDIDTVAAFGEADIERLLADAGIVRNRLKIDAAIF
                     NARQIQALQQEYGSFKNWLDAHHPRSKDEWVKLFKKHFKFVGGEIVGEFLMSTGYLKG
                     AHAESCPVYRETLKYHPKWLDAI"
     gene            7907..8548
                     /locus_tag="WGQ68_000268"
     CDS             7907..8548
                     /locus_tag="WGQ68_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEZ0714151.1"
                     /translation="MPSEKPMNRRTFLLGAGALLLTACGRKSARAHAKIPEGSTVLAL
                     GDSLTFGYGANPGESYPAQLQKLTGWNIVNGGVSGDTSAQALSRLPALLARKPKLVIV
                     GIGGNDFLRKVPKEQTRANIAKIIETVQKENIPAVLVGVPHITLGALFGHLSDHPLYE
                     DLSEEYGIPLFGGAWAEILGDNNLKSDQIHANGKGYRKFAEDLNQFLRKQGFR"
     gene            complement(8541..8771)
                     /locus_tag="WGQ68_000269"
     CDS             complement(8541..8771)
                     /locus_tag="WGQ68_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0714152.1"
                     /translation="MISACPGFIHSRLGRSVMPSEPAGRLFQKTPYRCNFMRLYGVGF
                     ALQTAYASRSSLVFQSVHDYINNLGADKPLFI"
     gene            8719..9081
                     /locus_tag="WGQ68_000270"
     CDS             8719..9081
                     /locus_tag="WGQ68_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714153.1"
                     /translation="MTDRPSLLWIKPGQAEIMEYCEPEEASDPYATYRCANLMAVLPL
                     FVVILVLLNIVFPLPAYPLAWLVPAGFMVLGGGFPLSLPLVALLVLTCCILLARCPPL
                     SRLLCYPCPNHPMSKNSA"
     gene            9112..11964
                     /gene="gcvP"
                     /locus_tag="WGQ68_000271"
     CDS             9112..11964
                     /gene="gcvP"
                     /locus_tag="WGQ68_000271"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEZ0714154.1"
                     /translation="MKLSELFNPNEFAARHLSFGDEAALLAAVGEKSMDDFVGNTVPQ
                     SIRMPSELDLPDALTEADALAKLKGIASKNVINKSYIGLGYYPTRVPNVILRNVLENP
                     GWYTAYTPYQAEIAQGRLEALLNFQQVCIDLTGFPVAGASLLDEATAAAEAMAMAHRV
                     GKVKSERFFVDERVYPQTLDVMKTRAKYFGFELVVGDFAKADEGEYFGALFQYVGKDG
                     DVQDLQDVIGRLKAKGTIVAVAADIMSLVLLKSPAELGADIALGNTQRFGVPMGFGGP
                     HAAYFAFKDEFKRSAPGRIIGVSKDASGKPALRMALSTREQHIRREKATSNICTAQAL
                     LANLAGMYAVYHGPEGVKRIANRIHALASAFADALVSDGLNVVHEVFFDTVTVDFGSK
                     EKADQVFAVALESGYNLRRVSDTQVAAAFHETSAYEDLVDLYRAFTGKDTFAFADDVK
                     GRLNTELLRQDDILQHHVFNRYHTEHEMLRYLKKLEDRDLAMNRSMISLGSCTMKLNA
                     TAEMLPITWAEFSDIHPYAPEAQTAGYRELLTDLENSLKAITGFDAISLQPNSGAQGE
                     YSGMLAIRRYQESQGERHRNICLIPKSAHGTNPATAAMLGLKVVVVDTDEHGNVNIDD
                     LKAKAEQHRDALSAIMITYPSTHGVYEEGIRDICRIVHENGGQVYMDGANLNAQIGIM
                     QPAEVGADVLHMNLHKTFCIPHGGGGPGMGPIGLKAHLAPFAPGHALTDTHSASADQT
                     AVAAAAFGSASILPITWMYLSMMGKQGMEQATRWALLNANYVAKRLSEDYPILYTGKN
                     GRVAHECIVDLRPLKAESGITETDIAKRLMDYGFHAPTVSFPVAGTLMIEPTESESKA
                     ELDRFIAALKQIKQEVLKVGRGEWPKEDNPLVNAPHTASDVTGEWAHPYSREEAVFPL
                     PFVREHKFWPSVNRVDDVYGDRNLVCSCPPMENYED"
     gene            complement(12429..13268)
                     /locus_tag="WGQ68_000272"
     CDS             complement(12429..13268)
                     /locus_tag="WGQ68_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c5 family protein"
                     /protein_id="HEZ0714155.1"
                     /translation="MKQLRDNKAQGSALFTLVSGIVIVIAVLYFLIKLAGSGSFGDVD
                     ATTEAATQTRIQPVGQLTMGDGIPVGERQGEQIFGKICIQCHAADSNVPNAPKLEHNG
                     DWAPRIAQGFDTLFQHALNGFNAMPAKGGAADLTDQELKRAITYMANKSGGSFPNPDE
                     AAPADNAASGTASAPADSAAPAEAKAEDKGAAAPAVGVDGKKVFEATCQVCHGGSIPG
                     IPGIGKKDDWAPRIKKGKETLHKHALEGFNAMPAKGGNAGLSDDEVKAAVDYMANQSG
                     AKF"
     gene            13553..13628
                     /locus_tag="WGQ68_000273"
     tRNA            13553..13628
                     /locus_tag="WGQ68_000273"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13586..13588,aa:Arg,seq:ccg)
     gene            complement(13693..14886)
                     /locus_tag="WGQ68_000274"
     CDS             complement(13693..14886)
                     /locus_tag="WGQ68_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0714156.1"
                     /translation="MYRHIEYYPGDPILSLVETFKNDPRPEKVNLSIGIYFDDEGKMP
                     VLESVSRAETARAATPVPSPYLPMEGLDTYRSAVQHLLFGKDNPALAQGRIVTVQTLG
                     GSGALKVGADFLHRWFPEARAYVSDPTWDNHRGIFESAGFEVGTYPYYDPAAVGVKFD
                     EMTAFFNTLPENSVLILHPCCHNPTGVDMSEQQWDEVLHIIKTRKLIPFMDIAYQGFG
                     GDLDSDAYAVRKAVEMELPLFVSNSFSKNLSLYGERVGGLSVVCPNKEEADLVFGQLK
                     FTVRRIYSSPPAHGAYIAADVMNSPELYALWQNEVYMMRDRIRAMRQKLYDVLTAQIP
                     DRDFTYFIKQRGMFGYTGLSVEQVRRLRDEFAVYLLDSGRMCVAGLNTSNTDYVARAF
                     AEVLK"
     gene            complement(14892..15995)
                     /gene="trmA"
                     /locus_tag="WGQ68_000275"
     CDS             complement(14892..15995)
                     /gene="trmA"
                     /locus_tag="WGQ68_000275"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HEZ0714157.1"
                     /translation="MSDYTQQLQDKKDHLKTLFAGLDVPEWEVYESPDKHYRMRAEFR
                     IWHEGGEMFYAMFEKGQKAGGASLIRCDRFEAASEAVNRLMPELIAVAAQSAELRNRW
                     YAVEFLSTLSGEMLVTMIYHKRLDNEWMQAAQALQQQLDISVIGRSRGQKIVLKQDYV
                     TETLKVGNRDFRYRQIEGSFTQPNAAVCQKMLEWACGAAEGLDGDLLELYCGNGNFTL
                     PLSEKFERVLATEISKTSVSAAQWNIEANRIGNIKIARLSAEEFTEAYTGKREFKRLK
                     DGGIALTDYAFSTIFVDPPRAGIDEETLKLVSQFDNIIYISCNPETLRTNLDTLAETH
                     AVERAALFDQFPFTHHIESGVLLKKKILGKSKR"
     gene            16138..17238
                     /gene="aroC"
                     /locus_tag="WGQ68_000276"
     CDS             16138..17238
                     /gene="aroC"
                     /locus_tag="WGQ68_000276"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEZ0714158.1"
                     /translation="MAGNTFGQLFTVTTFGESHGAGLGCIIDGCPPGLELSEADIQFD
                     LDRRKPGTSRHVTQRRETDQVEILSGVFKGKTTGTPIALLIRNTDQRSKDYGNIAQSF
                     RPGHADYTYWHKYGTRDYRGGGRSSARETAARVAAGAVAKKWLKEKFGTEITAYVTQV
                     GEKEIRFEGCEHISQNPFFAANHSQIAELENYMDSVRKSLDSVGAKLHIEAANVPVGL
                     GEPVFDRLDAEIAYAMMGINAVKGVEIGAGFDSVTQRGSEHGDELTPQGFLSNHSGGI
                     LGGISTGQDICVNIAIKPTSSIATPRRSIDINGNPIELATHGRHDPCVGLRAAPIAEA
                     MLALVLIDHALRHRAQNADVAVDTPDIARSDK"
     gene            17329..17754
                     /locus_tag="WGQ68_000277"
     CDS             17329..17754
                     /locus_tag="WGQ68_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant transport activator ProQ"
                     /protein_id="HEZ0714159.1"
                     /translation="MTQETALGAALKSAVQTMSKKKQTEMIADHIYGKYDVFKRFKPL
                     ALGIDQDLIAALPQYDAALIARVLANHCRRPRYLKALARGGKRFDLNNRFKGEVTPEE
                     QAIAQNHPFVQQALQQQSAQAAAETPSVEAEAAESSAAE"
     gene            complement(17828..19813)
                     /gene="parE"
                     /locus_tag="WGQ68_000278"
     CDS             complement(17828..19813)
                     /gene="parE"
                     /locus_tag="WGQ68_000278"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEZ0714160.1"
                     /translation="MAKNNQYSESSITVLKGLEPVKERPGMYTRTDSPTHICQEVIDN
                     AADEALGGFATAIDVQIHEDGSLSVRDNGRGIPVGLHPEEGVPVVELVFTRLHAGGKF
                     NKKDGGSAYAFSGGLHGVGVSVTNALSTRLEVTVKREGKIHRIVFAGGDVVEPLAQVG
                     KCAVKDSGTEVRVWPDGKYFESPNYSIPELERLLRAKAVLLPGVRVSLTRPVKGEDEA
                     HTQTWHYPDGLKSYLTDLIADAQEAVPIFSCENYISDGHNGDFSIGEGAAFALTWLEE
                     GSCANESYVNLIPTPLGGTHEAGLKQAVFNAVSNFINLHNLLPRGVKVQSDDVFGKTA
                     FVLSARVLDPQFQGQTKDKLTNRDALKLVATVSGDPLELWLNQNVDFGKKIAELAIRQ
                     AQARMRSVKKIEKKKGSGVAVLPGKLTDCESEDIRENELFLVEGDSAGGSAKLARDKA
                     TQAILPLRGKVLNSFEVHPDQLFGNAEIHDISVAIGVDPHGINDHPDLSGLRYGKIAI
                     LSDADVDGSHIQVLLLTLFYRHFPKLVADGHIYVAQPPLFRVDVNAQGKSKPARKFYA
                     LDQNELDGILERLQKEGVKETAYSISRFKGLGEMNPDQLKDTTMHPDTRRLLQVQIPE
                     GADDETRDIFVKLMGKGEAAARRAWMEREGDTAELDI"
     gene            complement(19880..20404)
                     /locus_tag="WGQ68_000279"
     CDS             complement(19880..20404)
                     /locus_tag="WGQ68_000279"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEZ0714161.1"
                     /translation="MLDREGYRPNVGIILINNRDEVFWGKRVREHSWQFPQGGIKPGE
                     SPETAMYRELYEEVGLLPQHVKIIGRTRDWLRYDVPNNWVRREWRGSYRGQKQIWYLL
                     RLTGRDCDVNLRATRHPEFDGWRWHQYWAPVDEVIDFKRDVYLGALKELSSRFLRGME
                     SYEDFAARQSSDNR"
     gene            20570..21865
                     /gene="serS"
                     /locus_tag="WGQ68_000280"
     CDS             20570..21865
                     /gene="serS"
                     /locus_tag="WGQ68_000280"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEZ0714162.1"
                     /translation="MLDIQLLRSNTAAVAERLAARGYKFDAARFNALEEQRKAVQVKT
                     EELQASRNSISKQIGALKGQGKHEEAQAAMDQVAQIKTDLEQAAADLEAVQKELDAWL
                     LSIPNLPHEDVPFGKDETENVEVRKVGTPREFDFEIKDHVDLGEPLGLDFEGGAKLSG
                     ARFTVMRGQIARLHRALAQFMLDTHTLKHGYTEHYTPYIVDDTTLQGTGQLPKFAEDL
                     FHVTRGGDESKTTQYLIPTAEVTLTNTVAGSIIPSEQLPLKLTAHSPCFRSEAGSYGK
                     DTRGLIRQHQFDKVEMVQIVHPEKSYETLEEMVGHAENILKALELPYRVITLCTGDMG
                     FGATKTYDLEVWVPAQNTYREISSCSNCEDFQARRMKARFKDENGKNRLVHTLNGSGL
                     AVGRTLVAVLENHQNADGSINIPAALQPYMGGVAKLEVK"
     gene            complement(21943..22941)
                     /locus_tag="WGQ68_000281"
     CDS             complement(21943..22941)
                     /locus_tag="WGQ68_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0714163.1"
                     /translation="MKIAIYGTKSYDREHFTRANRHFGFELEFFDFMLDAKTAKMAEG
                     AEAVCIFVNDDGSRPVLEKLAQIGVKTVALRCAGFNNVDLKAAEELGLKVVRVPAYSP
                     ESVAEHTVGLMLTLNRRIHKAYQRTRDANFSLEGLTGFNMYGKTAGVIGTGKIGIATM
                     RILKGFGMNLLAHDPFCNPEAEKIGGRYVDLDELYAQSDIITLHCPATPENHYMLNEA
                     AFDKMKDGVMIINTSRGGLIDSAAAIEALKRRKIGALGMDVYENERELFFEDKSNDVI
                     TDDVFRRLSSCHNVLFTGHQAFLTEEALGNISEVTLSNIREVGQTGDCGNAVRADG"
     gene            complement(23066..24142)
                     /gene="prfA"
                     /locus_tag="WGQ68_000282"
     CDS             complement(23066..24142)
                     /gene="prfA"
                     /locus_tag="WGQ68_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003790927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEZ0714164.1"
                     /translation="MKPSILEKLQQLGDRLEEVTHLLGQPEATSDMDNYRKLTREHAE
                     LTPVVEVFQNYQLAQSDLADAEEMLSDPEMKDFAAEEIEAAKAKINTLDTELQKLLLP
                     KDADDDKNIFIEIRAGTGGDEAALFAGDLLRMYSRYAERNRWQVEIVSANESELGGYK
                     EVIARIVGLGAYSRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPEADELEDIELN
                     PVDLRIDTFRASGAGGQHINKTDSAVRITHLPTGMVVECQDGRSQHANKAQAMKVLAA
                     RLNDAQKREAQAKEAAERKSLIGSGDRSERIRTYNYPQGRVTDHRINLTLHKLDFVMD
                     GDLAEITDALIAEHQAELLAAMGD"
     gene            complement(24232..25002)
                     /locus_tag="WGQ68_000283"
     CDS             complement(24232..25002)
                     /locus_tag="WGQ68_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEZ0714165.1"
                     /translation="MNFTDTHCHLADAALRENLPHILTAARKVGVKRFIVPATCPQDW
                     QSVAELSEMPSEHGQIRIALGIHPWFSDGIAERDCVRLEAMLARYPQAWVGEIGLDFY
                     DKTQTPQQRERQIQVFSRQLEIAQTLRRRVIIHNLKAAADIAAAVKRTGFTWGGIVHA
                     FSGSIEEAHVLAKLGFKIGIGSLLLNPNARKVRDTLKALNDGDFVLETDSPFMLKNTV
                     NTPANILRIAEIAAQIRGTGAAEIAAITERNADSLLRP"
     gene            complement(25117..26106)
                     /locus_tag="WGQ68_000284"
     CDS             complement(25117..26106)
                     /locus_tag="WGQ68_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEZ0714166.1"
                     /translation="MKQKIFVLYTGGTIGMTQSSEGLRPDTALVGQALAPFSNGMDFD
                     WHVCGTLIDSSAVQLQNWCAWLDIIAAALPQYDGVLVLHGTDTLAYTANLFALALQGL
                     DKPIVLTGSQWPYNAVGSDAPRNLATAVAAFPLGLKQTLIAFDGKLYPAVGSSKVSTE
                     TAAGFDNPHFGALAEWDETQGWHNVRIPNQDTADVSDGLKIRYPDPQAKIAVRTLIPG
                     FAVQELADGLGQLPAHALILQSYGHGNTPADEGFIRAVRDFTRQGKLLLNISQVRQGR
                     TAAVYAQGNAFRNSGIINGGKCNLETATALMTLAVSQGWGAEDVEKELARLKL"
     gene            complement(26173..26856)
                     /locus_tag="WGQ68_000285"
     CDS             complement(26173..26856)
                     /locus_tag="WGQ68_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0714167.1"
                     /translation="MFALLEAFFVEYGYAAVFFVLVICGFGVPIPEDLTLVTGGVISG
                     MGYTNPHIMFAVGMLGVLAGDGIMFAAGRIWGQKILKFKPIARIMTPKRYAQVQEKFD
                     KYGNWVLFVARFLPGLRTAVFVTAGISRKVSYLRFLIMDGLAALISVPVWIYLGEYGA
                     HNIDWLMAKMHSLQSGIFIALGVLAAALAWFWWRKRRHYQLYRAQLSEKRAKRKAEKA
                     AKKAAQKQQ"
     gene            complement(27045..28379)
                     /gene="glmM"
                     /locus_tag="WGQ68_000286"
     CDS             complement(27045..28379)
                     /gene="glmM"
                     /locus_tag="WGQ68_000286"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEZ0714168.1"
                     /translation="MAKKYFGTDGVRGEVGQFPITPDFVLKLGYAAGQVLVQHDTDQK
                     PTVLIGKDTRISGYMLEAALVAGFTAAGVNVIQTGPLPTPGVAYLTRALRLSAGVMIS
                     ASHNAYSDNGIKFFAEGGVKLSDEIELEIEAKIDEEMKTQPSARLGRARRISGADDRY
                     IEFCKSTFPTHSDLRGLKLVVDAANGAAYSVAPKVFHELGAQVVSIGDEPDGYNINEK
                     CGATHTKTLQAAVLQNEADYGIALDGDGDRLMMVDKNGKVYDGDSLIYVIAKARAREG
                     INIGGVVGTVMTNMAMEIALKEQGVDFCRAKVGDRYVLEQLNQRSWLIGGEASGHILC
                     MDKHNTGDGIISALQVLAALQTLNQDLATVCADWQPYPQTMINVRIGKGQNWQEASKD
                     VLAEVEKELEGKGRVVLRASGTEPVVRVMVEARQADWARDGAERIASAIGSL"
     gene            complement(28509..29366)
                     /gene="folP"
                     /locus_tag="WGQ68_000287"
     CDS             complement(28509..29366)
                     /gene="folP"
                     /locus_tag="WGQ68_000287"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEZ0714169.1"
                     /translation="MARHVWQAGRFEIGLDKPKIMGIVNLTPDSFSDGGAYSQNAQTA
                     LAHAERLLKEGADILDIGGESTRLGADYVSPEEEWARVEPVLAEVAGWGVPVSLDTRR
                     TVVMEKALALGGIDIINDVAALTDEGAVELLARQADTGICLMHMQGLPKTMQINPKYQ
                     DVVGEVARYLKARSAECIAAGIAPQRIILDPGFGSGFGKTLQHNIALMRHLPELMAET
                     GFPLLIGVSRKSTIGELTGEANAAERVHGSVAAALASVARGAQIVRVHDVKATADALK
                     VWEALGINL"
     gene            complement(29692..30585)
                     /locus_tag="WGQ68_000288"
     CDS             complement(29692..30585)
                     /locus_tag="WGQ68_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEZ0714170.1"
                     /translation="MIIVMSRRAAEADIAGVVAFIRSRGLREHISHGDERTVIGAIGD
                     DRVLSVREVQTLPEVEKAVRILDTWKTVSRENRAEDSRVAAKGVAFGGGETVRIAAEP
                     SVWGNADAVFLDPFFTSANLYDTSSADEGRGRCRRLAEQAASAHDAGKPVLVRVRNVR
                     HVEAALNAGADILYLGGGLMSDLAVLNEAGNLNIPLVLCKDKHHSAEDWLNAAEYVVS
                     RGNRHLILGESGVLNHTKGHPYRLDVESIVKVRQISHLPVIANITGLWNRDMPQEILY
                     GLAKAAGACGIVGTCFEKSGG"
     gene            30676..32790
                     /locus_tag="WGQ68_000289"
     CDS             30676..32790
                     /locus_tag="WGQ68_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HEZ0714171.1"
                     /translation="MDLLSVFHKYRLKYAVAVLTILLLAAVGLHASVYRTFTPENIRS
                     RLQQSIAHTHRKISFDADIQRRLLPRPTVILKNLTITEPGGDRTAVSVQETKIGLSWK
                     NLWSDQIQIEKWVVSSAELALTRDGKGVWNIQDLIDSQKRQASVNRIIVENSTVRLNF
                     LQEQLILKEINLNLQSPDSSGQPFESSGILVWGKLSVPWKSRGLFLSNGIGPPEISPF
                     HFEASTSLDGHGITISTTGSPSVRFNAGGADAAGLGLRADTSFRNLHLTAQIPTLALR
                     NNSIKIETVNGAFTAGGEYAQWDGSFKLDKANLHSGIANIGNAEISGSFKTPRHQTNF
                     SLNSPLVWTENKGLDAPRLYVSTLQDTVNRLPQPRFISRLDGSLSVPNLQNWNAELNG
                     TFDRQTVAAKFRYTHEDAPHLEAAVALQKLNLTPYLDDVRQQNGKIFPDTLAKLSGDI
                     EAHLKIGKVQLPGLQLDDMETYLHADKDHIALSRFKSGLYGGHTEGGISIANTRPATY
                     RLQQNASNIQIQPLLQDLFGFHSFSGNGDAVIDLTAGGETRKELIRSLQGSLSLNISN
                     GAWHGIDMDNILKNGISGKTADNAAPSTPFHRFTLNSEISDGISRHIDTELFSDSLYV
                     TSNGYTNLDTQELSEDVLIRNAVHPKNKPIPLKITGTVDKPSITVDYGRLTGGINSRK
                     EKQKILEDTLLEQWQWLKPKEP"
     gene            complement(32855..34333)
                     /gene="ubiD"
                     /locus_tag="WGQ68_000290"
     CDS             complement(32855..34333)
                     /gene="ubiD"
                     /locus_tag="WGQ68_000290"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HEZ0714172.1"
                     /translation="MKYKDLRDFIAMLEQQGKLKRVAHPVSPHLEMTEIADRVLRAEG
                     PALLFENPVKPDGTRYDYPVLANLFGTPERVAMGMGADSVSKLREIGQTLAYLKEPEP
                     PKGIKDAFSKLPLLKDIWSMVPNVVKNAPCQEIVWEGEDVDLYQLPIQHCWPEDVAPL
                     VTWGLTVTRGPHKKRQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEFHKLNPDTPYP
                     VAVVLGCDPATILGAVTPVPDTLSEYQFAGLLRGSRTELVKCIGNDLQVPARAEIVLE
                     GVIHPNETALEGPYGDHTGYYNEQDHFPMFTVERITMRENPIYHSTYTGKPPDEPAVL
                     GVALNEVFVPLLQKQFPEITDFYLPPEGCSYRMAVVSMKKQYAGHAKRVMMGCWSFLR
                     QFMYTKFIIVVDDDVNVRDWKEVIWAVTTRMDPVRDTVLVENTPIDYLDFASPVSGLG
                     GKMGLDATNKWPGETDREWGRVIKKDPAVTAKIDGIWEELGL"
     gene            complement(34353..34868)
                     /locus_tag="WGQ68_000291"
     CDS             complement(34353..34868)
                     /locus_tag="WGQ68_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2199 domain-containing protein"
                     /protein_id="HEZ0714173.1"
                     /translation="MYTCTSCGENHEEMPAIGFTAPYPYNQLSEEERIAYQAECDSDF
                     CIIRYTDQTDRFIRAVLPIPIIGHQETLEYGVWVSVSEKSFNDYQSRFYDNLENAVYF
                     GMICNQLPPYETDTFGLHCNVVTQPDGQRPLLQLHQGGEHPLVRDFYHGIEYAEAQAR
                     IGAVSELDCSG"
     gene            complement(34875..35375)
                     /locus_tag="WGQ68_000292"
     CDS             complement(34875..35375)
                     /locus_tag="WGQ68_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714174.1"
                     /translation="MVFVNPLYYGREAGWFVWLAAAMCGLWLLRALTAKLPQQRYAAL
                     FITLFFAAVLVFGRHDSMYWYPVLVNLMMLAVFGGSLFAGQTVIEKLARLQQPDLPEK
                     AVRYTRRVTQLWCVFFIINGTLAALLARLGRYDWWAVYTGVIAYVLMGMLFAGEWLYR
                     KLVLKV"
     gene            complement(35607..36176)
                     /locus_tag="WGQ68_000293"
                     /pseudo
     CDS             complement(35607..36176)
                     /locus_tag="WGQ68_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691695.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
     gene            36223..36819
                     /locus_tag="WGQ68_000294"
     CDS             36223..36819
                     /locus_tag="WGQ68_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0714175.1"
                     /translation="MKITHCKLKKKVQKELLHFFVLEVTARSAADILGIHPNSAALFY
                     RKIRTVINHHLVLAADEVFEDPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRV
                     YTVVVNNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYSGLARTFPRIGSATGCAD"
     gene            36764..37900
                     /locus_tag="WGQ68_000295"
                     /pseudo
     CDS             36764..37900
                     /locus_tag="WGQ68_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247614.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
     gene            37954..38029
                     /locus_tag="WGQ68_000296"
     tRNA            37954..38029
                     /locus_tag="WGQ68_000296"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:37987..37989,aa:Ile,seq:cat)
     gene            38104..38862
                     /locus_tag="WGQ68_000297"
     CDS             38104..38862
                     /locus_tag="WGQ68_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0714176.1"
                     /translation="MKKVSVLIVAKNEANHIQECIESCRFDKEVIVIDDYSTDNTAEI
                     AEGLGAKVFRRHLNGDFGAQKTFAIEQAGGEWVFLIDADERCTPELSDEISKIVQTGD
                     YAAYFVERRNLFPNHPATHGAMRPDSVCRLMPKKDSSVQGKVHETVQTPYPKRRLKHF
                     MYHYTYDNWEQYFNKFNKYTSISAKKYREQGKPVRFVRDIILRPIWGFFKIYILNKGF
                     LDGKMGWIMSVNHSYYTMIKYVKLYYLYKSGGKF"
     gene            38863..39927
                     /locus_tag="WGQ68_000298"
     CDS             38863..39927
                     /locus_tag="WGQ68_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0714177.1"
                     /translation="MEKEFRILNIVSAKIWGGGEQYVYDVSKALGLRDCTMFTAVNKN
                     NELMHRRFSEVSSVFTTRLHTLNGLFSLYALTRFIRKNRISHLMIHTGKIAALSILLK
                     KLTGVRLIFVKHNVVANKTDFYHRLIQKNTDRFICVSRLVYDVQTADNPFKEKYRIVH
                     NGIDTDRFPPSQEKPDSRFFTVAYAGRISPEKGLENLIEACVILHRKYPQIRLKLAGD
                     GHPDYMCRLKRDVSASGAEPFVSFEGFTEKLASFYRQSDVVVLPSLVPEAFGLSLCEA
                     MYCRTAVISNTLGAQKEIVEHHQSGILLDRLTPESLADEIERLVLNPEAKNALATAAH
                     QCVAARFTINHTADKLLDAI"
     gene            40314..41651
                     /locus_tag="WGQ68_000299"
     CDS             40314..41651
                     /locus_tag="WGQ68_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0714178.1"
                     /translation="MSNHTSWSSKIGFVLAAAGSAIGLGAIWKFPYTAGTNGGAVFFL
                     LFLIFTILVALPVQLAEFYIGRTGGKNAVDSFRALRPGTQWLWVGRMGVAACFILLSF
                     YSVVGGWVLNYVVHSFTGAVHTGADFEALFGATISNPAGSLSYQALFMLITVWVVKGG
                     ISDGIEKANRYLMPGLFILFIALAIRSLTLPGAMEGVSFLLKPNWSYFKADTMITALG
                     QAFFALSIGVSAMITYASYLGKDQDMFRSGHTIMWMNLLVSLLAGLVIFPAVFAFGFE
                     PSQGPGLIFIVLPAVFMKMPFGTVLFAVFMLLVVFATLTSAFSMLETVIASTIRQDER
                     KRKKHTWLIGTAIFIIGIPSALSFGVWGEFKVFGKTIFDLWDYVISAVIMPIGALSVS
                     IFTAWIQDKQSVLKDAGAGSTVPRAVLLLWLNTLRYLAPIAIIIVFINSLDIL"
BASE COUNT         9746 a        11509 c        10654 g         9819 t
ORIGIN      
        1 aaccccatca cacagattta cggtaaaaag ccgtccgaat gaattcttga acacaattcg
       61 gacggcttta attttcaaca aggcgattaa ttcaataata ccagattaaa acttccattc
      121 cagcgatacg gcatagttgc ggccgggggc gtagtagcgt tccagaccct gatttgctga
      181 tttagtattc gatacgctta aagagttaat agtcgatctc ggattgatgc cgcgcaagct
      241 gtcccaagta tggtatttcc ggttaaacag attataaacg cccgcccgta aagtcatacg
      301 ttttaccggt ttgtagtagc cgaatacatc cacaacagtt gccgctttat tcaaccaacg
      361 gaagtcgatt ttatcgtcac gatactgcaa ggcaacagca caatcatctg tttccttacc
      421 tgtccaagga tcgagtttat agcttgtgca atagctcttt tgttcaatta cttgggcatc
      481 tttggctttt ttcctgccca tatagctgac acgggagaaa ataccccaac gctcatcagg
      541 agactcataa tcgaatccaa gcaccaactt cagcggctgg atggacaaca aactgccgcg
      601 ttcaccggac aatttgcctt tactataacc caatgcgccg gatactttaa aaccttccgg
      661 cacagacatc acttgatgta ggttcaactc acctttaaat tcaactcccc tcaccctggc
      721 tttatcaata ttgaccattt gttggccgat ttcggtaagc gtagggttac aatatttagg
      781 attataatag caactatgtt cattataata ctcattcggc acctgatacg ttttttcttc
      841 ctcaaataag aaattacggt aacgggtctg atacaggcta aaatccagca atcctttgtc
      901 attctttcca cgcagtgaca gagtttggtt tagactgcgc tctgctttca acgatgggtt
      961 agcaatccaa ttaccgtaag ggctggtata agtaaaatac atttccgatg ctgtcggcac
     1021 gcgatgcccc gtattgattt ggtaagctgc acgccaagta tcgttaaaga ggtagtccaa
     1081 acctaaaacg ccgttccatg tattaaaact tcttgcggac ggattaccgt cattaacaca
     1141 ggtactcaga caaggcgtat ctggcttgaa agcctgcggt tttaccttct catggtcgta
     1201 gcgcacgcct gcatttacag ataacttgtc gccaaaagcg attttatcct gcaatgaaaa
     1261 accgatttga tccgtcttca taggacgcat aatagtgtag tgtttacggc cagttatatt
     1321 gcctgttgaa ttaaaatcta aatcatcatt tacattttca aagtcgcgac gactggcaaa
     1381 agtcttaaaa gataatctgt gttcaccttt ccacagtgaa aaaggctggc tgtccaaacg
     1441 gaaggtaatg cgcttgtatt tggtatccat attccgctcg tctctatcag ccataaagcc
     1501 gttgtgatac tgataacctg tgcgccaatc tccggtacga accatatcgc ctttatagtt
     1561 tatcgcccca ttgcgggtct tctgatagtc caagtccgtg cgcagcaagg aaaggatgga
     1621 agagtcgggc gtccattcgt ggttcagatt aatgttgctg cgtttctgta cgtcgtccgc
     1681 ttcccgccaa taacttgtca gggagtaagt tttttcataa gtgtaattgc tgttattttg
     1741 cccattaaca gaaaagccaa aacggtgtgc atcaaccggt tgccagccta atttcaccaa
     1801 ataactgtga ttattgtgtt tggatggatc tggatgaatg cgtgccgagc cacgctctgc
     1861 ctgatcctgc ttatccaatt cgccaaaccc tacaaaatca acgacatccc ccccgctttt
     1921 catttcgtgc ccgcgtcggc gggaatacaa aaccacggca tcaaaaacat tgcccgcata
     1981 gccgaaacct gcggtattcg cccactctct gtttttactt gcataaccac tgcgaagaag
     2041 tgcaccgaaa ttttgttccg gcaaaacaat atcccgtgca tcaagcgtgc ggtagttcac
     2101 attaccaccc aaagagccgc tgccgctttc aaatgaatcc gcacctttcg taatatccac
     2161 acccttaacc aattcggtat cgatgcttaa acgggaatta ttgaagttgc cgtaacgtgc
     2221 ataaagtgaa ttttcttccg aactgggcaa tgccacgccg tcgatactga taccgacacg
     2281 attgtcttcc accccgcgca tggcaaagcc tttcatacgg cgaccatcat cggatacacc
     2341 gacatctgat gaataacgca ccaaatcgcg tgtatcccga atcatttgct cttgaatggt
     2401 tttagccttt actcgttcca cagtggtaga tgcattgtgc ttacctttaa cgcgcactgc
     2461 ttttatctct gccttaacgg gtgtggtttc aattgcagct tcatccgctg ccaagaccgg
     2521 attgccgaaa atactgccga ccagcgcggc gatagggagc atttgtaatg gtttcatatt
     2581 atcaactcaa gatgtatgga ttgttcatcc atcggttagc gaataataga ttttatgata
     2641 atcattaata tttaataaga cagtaatcca tgtaaacaaa gtcgcgccgt gtaattaaag
     2701 gtccctgcaa acagctatgc cgagaccttg tttatttggt tcgattattt tttagtgcct
     2761 gtgcggcatc attccttccg gtgcttcggc atcggctgcc aagccgaagg tttcgcgcaa
     2821 cacgactttg tagaatgcaa aggcttcgcg cgcgccttgg atggcttccg cttcggcttc
     2881 gggagtcagg ttcaaagcgt tcagatgctc gacgaaggcg cgccagtgtt tgccgcgccc
     2941 gtcgggatgg ggtgcgaggt ggcgcgcgcc gtgttcgccg ttgtaatcga gtttttgggc
     3001 gtgtttgaac aaaaatgccg cgcccaaatt ggatccttcg gcgcaataaa gccagccgat
     3061 tgctttgttg ccggtttcgt gtggcaattc tttgtcgaat ttgtaaggct cttcgcccaa
     3121 atctgcaagg tcttgcgtta cggcatcgta tcgcgccatg tattccagct cgggaatggc
     3181 tttgtttaat tcggcatctt tatagatgtg gtcgacggcc ttgtggaaaa cggattggag
     3241 tttcaaaaat ttgatgtagt tttctttgct gacaaacggt tggacagaca taacgagatt
     3301 atccacgctg tcgtgaaccg ccgtggtatc cgccttcaga cgtttggcaa acgtcagggc
     3361 ttgattttcg gtttcactca taagttttcc tttgtcggta agggataagg atgcggggcg
     3421 ggcaaaatcc gcacttgccc gcatatatag tggattatat agtggattaa caaaaaccag
     3481 tacggcgttg cctcgcctta gctcaaagag aacgattctc taaggtgctg aagcaccaag
     3541 tgagtcggtt ccgtactatt tgtactgtct gcggcttcgc cgccttgtcc tgatttttgt
     3601 taatccacta taaaaataat aaagaatcat aaacgaaatt tattatcaca tatttttgga
     3661 aaaaatatca tttgcgtgat gtttttaagc aggtatttta ctattcttta cagaatcggg
     3721 attttatcaa atgggttcgg cagtcggcgg acagccgctc aaaaaatatt tttgccggac
     3781 accaagggtt tgttcatact gccgaacctg ccggttttgc atcctgattg ggtgtatcgc
     3841 cttttttcct ttataatgcc gccacttata tttgccactt tcccgatgaa gccgtttgcc
     3901 gaaaatatcc cccacagcct tcgcggcaac tgccgcgacg aagccctgcc gccgcatacg
     3961 gtagattgcc cggaatgcgg ctgccgcgcg gatgtacccc ggttggacag gggagaggcg
     4021 gcgttctgtc cccgttgcgg ctataagctc ttcagggtgg gcaggcatcc tttttccgcc
     4081 ccgcccgctt atgcggcggc ttcgctgatt ttgatggcgt ttgcttacgg tatgacgtat
     4141 atcgaggtcg ggataccggg tgcggcatcc gtcctttcgc tgcccgagat gatgcgcctg
     4201 atggtgtttc aggattatgg ttttttggcc gaagtgatgt ttgtgctgac cttcggcgcg
     4261 ccggttctgt ttctgctgct gtgcctgtat gtctatgccg cgctgatacg gaaacaggcg
     4321 tatcctgcgc tgcgtttggc aacgcgtgtg atggtgcgct tgagacaggc gatgatggtg
     4381 gatgtgtttt ttgtttccac tttggtggcg tatatcaagc tctcgtctgt ggcagaggtt
     4441 cgcttcggat cggcgtttta tctgatgttc gcgctgtcgg ttatgctgat tcggacttcg
     4501 gtatcggttc cccagcattg ggtgtatttt caaatcgggc ggctgacggg ggataatgcg
     4561 gttcagacgg catcggaagg taaaacctgt tgcagccgtt gcctgtattt ccgcgacagt
     4621 gccgaatccc cctgcggcgt gtgcggtgcg gaactgtacc gccgacggcc gaaaagtctg
     4681 agtatttcgt cggcgtttct gacggcggcg gttattttgt atttccctgc caatatcctg
     4741 ccgattatga tttcgtccaa tcctgccgcc acggaggtca ataccatcct taacggcatc
     4801 gcttatatgt gggacgaggg cgacaggctg attgcggcgg ttattttcag cgcgagtatt
     4861 ttggtgccgg tactgaagat tgcggcaatg tcggttttga ttgcgtccgc ccgcttcgct
     4921 ttgccaacgg gtgcaaagaa attgtcgcac ctctaccgca tcaccgaagc ggtcggccgc
     4981 tggtcgatga ttgatatttt tgtgattatt attttgatgt gttcgttcca cacttatgcc
     5041 gcgcgcgtca ttccgggcag tgcggcagtc tatttctgcc tggtcgtgat tctgacgatg
     5101 ctgtccgcct attatttcga cccgcgcctg ctttgggaca aacgcgcttc agacggcatt
     5161 gctttcaacg aaacggaaaa acatgactga caacagccct cctccaaacg gacacgccca
     5221 agcacgcgtc cgcaaaaaca acaccttgct ctccgccgtc tggctggtcc cgctgattgc
     5281 gctgattgcc ggtggctggc tgtggattaa ggaaatccgc aacagggggc ctgtggttac
     5341 gctcttgatg gacagcgcgg aaggcattga ggtcaacaat acggtcatta aggtattgag
     5401 catcgatgtc ggacgcgtta cccgaatcaa actgcgcgac gaccaaaaag gcgtggaagt
     5461 aaccgcccaa ctcaatgcgg acgtatccgg cctcatccgc agcgataccc agttttgggt
     5521 ggtcaagccg cgtatcgacc aaagcggcgt aaccggtttg ggtacgctgc tttcgggttc
     5581 gtacatcgct tttacacccg gcaaaagcga cgaggcaaaa gacgtgttcc aagtgcagga
     5641 cattccgccc gttaccgcca tcgggcaaag cgggctgcgc ttgaatttga ttggtaaaaa
     5701 cgaccgcatc ctcaacgtca acagccctgt tttgtatgaa aactttatgg tcgggcaagt
     5761 cgaaagcgcg catttcgacc cgtccgacca aagcgtgcat tacaccatct tcatccaaag
     5821 ccccaacgac aaactgattc attccgccag ccgtttttgg ctggaaagcg gcatcaatat
     5881 cgaaaccaca ggcagcggca tcaaactcaa ttccgcccct ctacctgccc tgctgtcggg
     5941 cgcgatttca tttgattcgc cgaaaaccaa aaacagtaaa aacgttaaaa gcgaagacag
     6001 cttcacgctt tacgacagcc gcagcgaagt cgccaacctg cctgacgacc gctcgctgta
     6061 ctacaccgcg tttttcaaac aatccgtgcg cggactgacc gtcggttcgc ccgtcgagta
     6121 caaagggctg aatgtcggcg tggtttccga tgtcccttat ttcgaccgca acgacagcct
     6181 gcatttattt gaaaacggct ggattcccgt acgcatccgc atcgagcctt cccgtttgga
     6241 aatcaatgcc gacgaacaaa gcaaagagca ttggaaacaa caatttcaga cggctttaaa
     6301 caaaggcctg accgccacca tctccagcaa caacctgctg accggcagca aaatgattga
     6361 gttgaacgat cagccttcgt cttcgcccaa gctgcgaccg cataccgttt atgcaggcga
     6421 taccgtcatc gctacacaag gcggcggttt ggacgatttg caggtcaaat tggcggattt
     6481 gctggacaag ttcgacaaac tgcctttaga taagacggtt gccgaattga acggttcgct
     6541 tgccgagctc aaatccacac tcaaatctgc caatgccgcc ctaagctcca tcgacaaact
     6601 ggtcggcaaa ccgcagacac aaaacattcc gaacgaactg aaccaaaccc tgaaagagtt
     6661 gcgcacaacc ctgcaaggcg tatcgccgca atcgcctatc tacggcgacg tacaaaatac
     6721 gctgcaaagt ttggacaaaa ctttaaaaga cgttcaaccc gtgattaata ctttgaaaga
     6781 aaaacccaac gcgctgattt tcaacagcag cagcaaagac cctatcccga aaggaagccg
     6841 ataatgcgcc ttttcccgat tgccgccgcc ctgtcgcttg ccgcctgcgg tactgtgcaa
     6901 agcacacaat atttcgtgtt gcccgacagc cgctacatcc gtcctgcaac gcaaggcggc
     6961 gaaactgccg tcgaagtccg tcttgccgaa ccgctcaaac gcggcggact ggtctatcaa
     7021 accgacccct accgcctcaa caccgcacaa aaccacgtct gggcagacac cttggacgat
     7081 atgctcgaag cggcgttgag caatgcattc aaccgtttgg acagcacacg catctttgtt
     7141 cctgcctcac gcagcggcag taccgaaaaa tggacggtct atatcgacgc attccaaggc
     7201 agctacacgg gcaaaaccct catcagcggc tacgccgtcc tacccgacgg tacgaacaga
     7261 cccttccata tcgaaaccga acagcagggt gacggctacg ccgcgatgac cgccgcactc
     7321 gaacagggac tgaaacaggc ggcgcaacag atggtcgagt aaaccgtgaa ctattgcgaa
     7381 tttgccgcct cacttcccga aaacaccgat aacccgaaca aacattacca cgacacgcaa
     7441 tacggttttc cgattgacag cgacaacgga ctgtttgaac ggctggtgtt ggaaatcaat
     7501 caggcaggat taaactggac gctgatgctg aagaagcagc aggcatttca gacggcattt
     7561 aaaggtttcg acatcgatac ggttgccgca tttggcgaag ccgatattga gcggctgctt
     7621 gccgacgctg gcatcgtccg caaccgcctg aaaatcgatg ccgccatttt caatgcacgg
     7681 caaatccaag cgttgcaaca agaatacggc tcgttcaaga actggctcga cgcgcaccat
     7741 ccgcgaagca aagacgaatg ggtcaaactc tttaaaaaac atttcaaatt tgtcggcggc
     7801 gaaatcgtcg gcgaatttct gatgagtacc ggctacctca aaggcgcgca cgccgaaagc
     7861 tgtccggttt accgtgaaac cctgaaatac cacccgaaat ggctcgatgc catctgaaaa
     7921 accaatgaac agaagaacct tcctcctcgg cgcaggcgcg ttgctcctta ccgcctgcgg
     7981 cagaaaatcc gcccgagccc acgccaaaat tcccgaagga agcaccgtac ttgccttggg
     8041 cgattcgctc accttcggct acggcgcaaa ccccggcgaa tcctaccccg cgcaactgca
     8101 aaaactgacg ggttggaata ttgtcaacgg cggcgtatcg ggcgatacat ccgcccaagc
     8161 cctgtcgcgc ctgcccgcgc tgttggcacg caaacccaag cttgtgattg tcggcatagg
     8221 cggcaacgac tttctgcgca aagttcccaa ggagcagacc cgcgccaata tcgcgaaaat
     8281 catcgaaacc gtacagaagg aaaacatccc cgccgtcctc gtcggcgtgc cgcacatcac
     8341 actgggtgcg ttgttcgggc atttgagcga tcatccgctg tatgaggatt tgtccgagga
     8401 atacggcatt ccgctgttcg gcggcgcgtg ggcggaaatt ttgggcgata ataatctgaa
     8461 atccgaccaa atccacgcca acggcaaagg ctatcggaaa ttcgccgaag atttgaatca
     8521 atttttgaga aaacaggggt ttagataaac aaaggtttat ccgcacccaa gttgtttata
     8581 taatcatgaa ccgactggaa caccaaactg cttcgggacg catatgccgt ctgaagtgca
     8641 aaacctacgc catacagccg catgaagttg cagcggtatg gcgttttttg aaaaagacgg
     8701 cctgccggtt cggacggcat gaccgaccgt ccgagcctgc tgtggataaa gcccggacag
     8761 gctgaaatca tggaatattg cgaacctgaa gaagcgtccg acccgtacgc aacatacagg
     8821 tgtgccaacc tgatggcggt gctgccgctg tttgtcgtga ttttggttct gctcaatatt
     8881 gtttttccgc ttccggcgta tccattggct tggctggtgc ctgcaggttt catggttttg
     8941 ggcggcggct ttcccttatc gctgccgctt gtggcgctgc ttgtcctgac ctgctgcatt
     9001 ctgctggcgc gttgtccgcc attatcccgt cttttgtgct acccttgccc gaatcatccg
     9061 atgtctaaaa attctgcctg atggcagccc tacaaacccg aaggagtaga aatgaaactg
     9121 tccgaactgt tcaacccgaa cgaatttgcc gcgcggcatt tgagtttcgg cgacgaagcg
     9181 gcgttgcttg ccgctgtcgg cgagaaaagt atggacgatt ttgtcggcaa caccgtgccg
     9241 caaagcatcc gtatgccgtc cgaactcgat ttgcccgatg ccctgaccga agcggacgcg
     9301 ttggcaaaat tgaaaggcat tgcgtcgaaa aacgtgatca acaagtccta tatcggttta
     9361 ggctattacc cgacccgcgt gccgaatgtg attttgcgca acgtattgga aaatccgggc
     9421 tggtataccg cctacacgcc gtatcaggcg gaaattgccc agggtcgttt ggaagctttg
     9481 ttgaacttcc aacaagtgtg tatcgatttg accggtttcc ctgtggcggg cgcgtctttg
     9541 ctggacgaag cgaccgccgc cgccgaagcg atggcgatgg cgcaccgcgt gggcaaggta
     9601 aaatccgagc gtttctttgt ggacgagcgc gtgtatccgc aaactttgga cgtgatgaaa
     9661 acccgtgcca agtatttcgg cttcgagctg gtggtcggcg attttgccaa agcggatgaa
     9721 ggcgaatact tcggcgcgct gttccaatac gtcggcaaag acggcgatgt gcaagacttg
     9781 caggacgtta tcggccgtct gaaagccaaa ggcacaatcg tggccgttgc cgccgacatt
     9841 atgagcttgg ttttgctgaa gtctccggct gaattgggcg cggatattgc tttgggcaac
     9901 acccaacgct tcggcgtacc gatgggcttc ggcggtccgc atgctgctta tttcgcgttt
     9961 aaagacgagt tcaaacgttc cgccccgggc cgtatcatcg gcgtatccaa agacgcatcg
    10021 ggcaaacctg ccttgcgcat ggcgttgtcc acccgcgagc aacacatccg ccgcgaaaaa
    10081 gccacgtcca atatttgtac cgcacaagca ttgctggcga acttggccgg tatgtatgcc
    10141 gtttaccacg gccctgaagg cgtgaaacgc atcgccaacc gcattcacgc gctggcttct
    10201 gcctttgccg atgcgctggt ttcagacggt ctgaatgtgg ttcacgaagt ctttttcgat
    10261 accgttaccg ttgatttcgg cagcaaagag aaagcagacc aagtgtttgc cgttgctttg
    10321 gaatcgggtt acaacctgcg ccgcgtcagc gatactcaag ttgcggctgc attccatgaa
    10381 acatcggcat acgaagattt ggttgatttg taccgtgcgt ttaccggcaa agatacgttc
    10441 gcatttgccg atgatgtcaa aggccgtctg aacaccgaat tgctgcgtca ggacgacatt
    10501 ttgcaacatc atgtgttcaa ccgttaccac accgaacacg aaatgttgcg ctatctgaaa
    10561 aaactcgaag accgcgactt ggcgatgaac cgcagcatga tttcattggg cagctgcacc
    10621 atgaaactca atgcgactgc ggaaatgttg ccgattactt gggccgagtt ctccgacatc
    10681 cacccctacg cccccgaagc ccaaaccgcc ggctaccgcg aactcctgac cgatttggaa
    10741 aacagcctga aagccatcac cggttttgac gcgatttccc tgcaaccaaa ttccggcgcg
    10801 cagggcgaat acagcggtat gctcgccatc cgccgctatc aggaatccca aggcgagaga
    10861 caccgcaaca tctgtctgat tcccaaatcc gcccatggca ccaaccccgc caccgccgcc
    10921 atgctcggtt tgaaagtcgt cgtcgtcgac accgacgaac acggcaacgt caacattgac
    10981 gatttgaaag ccaaagccga gcaacaccgc gacgctttgt ctgccatcat gattacctat
    11041 ccgtccaccc acggcgtgta cgaagaaggc atccgcgaca tctgccgcat cgtccatgaa
    11101 aacggcggac aggtttacat ggacggcgcc aacctcaacg cccaaatcgg catcatgcag
    11161 cctgccgaag tcggcgcgga cgtgttgcac atgaacctgc acaaaacctt ctgtatccct
    11221 cacggcggcg gcggtccggg catgggtccg attggcttga aagcccatct ggctccgttc
    11281 gcaccgggac acgctttgac cgacacccac agcgccagtg ccgaccaaac cgccgttgct
    11341 gctgcggcct tcggttctgc gtccatactg ccgattactt ggatgtacct gagcatgatg
    11401 ggcaaacaag gcatggaaca ggcgacacgc tgggcattgc tcaacgccaa ctacgttgcc
    11461 aaacgcctga gcgaagacta tccgattctg tatacaggca aaaacggccg cgtcgcgcat
    11521 gaatgtatcg tcgatttgcg tccgctcaaa gccgaaagcg gcatcactga aaccgacatc
    11581 gccaaacgcc tgatggacta cggcttccac gccccgacgg tttccttccc cgttgccggt
    11641 acgctgatga tcgagccgac cgagagcgaa agcaaagccg aactcgaccg cttcatcgcc
    11701 gccctgaaac aaatcaaaca ggaagtgctg aaagtcgggc gcggcgaatg gccgaaagag
    11761 gacaatccgc tggtcaacgc tccgcatacc gcatccgatg taaccggcga atgggcgcat
    11821 ccgtactccc gtgaagaagc cgtctttccg ttgccattcg tccgcgaaca caaattctgg
    11881 ccgagcgtca accgtgtgga cgacgtgtac ggcgaccgca atctcgtgtg cagctgccca
    11941 ccgatggaaa attatgaaga ctgaccgttg aaaccccgaa gacaaatgcc gtctgaaaca
    12001 ttttcagacg gcattttcgt caacggcaga ctggttgcac caatacacgt atctcgacta
    12061 taactttaaa acaaatgagt taaaccagta tccatacatc agctttttta tcatcctact
    12121 ttttattcat ccgatcgtgc aaacagattt caaagatgaa aagcctattc acaccctttg
    12181 atgtcatttc cacacggaca aacaaatata gtggattaac aaaaaccagt acagcgttgc
    12241 ctctccttag ctcaaagaga acgattctct aaggtgctca agcaccaagt gaatcggttc
    12301 cgtactatct gtactgtctg cggcttcgtc gccttgtcct gatttttgtt aatccactat
    12361 aaattcccat aaaaaaacgg agcagatacc tgccccgttt ttatttaatc cgaaatttta
    12421 atctaaattt agaattttgc accggattgg tttgccatat agtcaacagc cgctttgact
    12481 tcgtcatcgc tcaaaccggc attgccgcct ttggccggca tcgcgttaaa gccttcaagg
    12541 gcgtgtttgt gcaaggtttc tttgcctttt ttgatacgcg gtgcccaatc gtcttttttg
    12601 cctatgccgg gaataccggg aatcgaaccg ccgtggcaca cctgacaggt tgcttcgaag
    12661 acttttttac cgtcaacgcc gaccgcaggg gctgccgcac ccttgtcttc tgccttcgct
    12721 tctgccggag ctgcactatc ggcaggagca gaagctgttc ctgaagcggc attgtcggca
    12781 ggcgcagcct catcaggatt cgggaaagaa ccgccgcttt tgttcgccat gtaagtaatc
    12841 gcccgtttga gttcctgatc ggtcaggtct gccgcaccgc ctttggcagg catggcgtta
    12901 aagccgttca gcgcgtgttg gaacaaggta tcgaagcctt gcgcgatacg cggtgcccaa
    12961 tcgccgttgt gttccagttt cggagcgttc ggcacattgc tgtccgccgc gtggcattgg
    13021 atacagattt tgccgaaaat ctgttcgcct tggcgttcgc cgacggggat gccgtcgccc
    13081 atcgtcaatt gtccgacagg ctggatacgg gtctgcgttg ctgcttccgt agtggcatcg
    13141 acatcgccga acgagccgct gcccgccagc ttaatcagga aataaaggac tgcaataaca
    13201 ataacgatac cgctcacaag ggtaaacagt gcagagcctt gggctttgtt gtcgcggagt
    13261 tgtttcattt ggtaggcctc gccgtcaggt taggttgtgc tgtaaattat agtttggtgt
    13321 gttaaacgca gttaacaata ttttgctgga ttatactgaa ttcacagggt ctttccaatc
    13381 gctatcattg aaaatatgaa aaaatttgcc aacggtatct gtataaaaca aataatcctt
    13441 tgaaaataat ggtttatcct caagaaaact ctccttatgc cgccatacgc cgcctgccgg
    13501 cgcaagataa cctttgccaa tttgcagaat ttacgttaac cttacgtttt acgcacccat
    13561 agctcagttg gaagagtgtc agtttccgaa gctggaggtc acaggttcga tccctgttgg
    13621 gtgcgccaat tataaagaga ccgtctgaaa gataaatatt tttcagacgg tcttttgact
    13681 tacttcaaac tcttatttca agacttccgc aaatgcgcgg gcaacatagt cggtattcga
    13741 cgtattcagt ccggcgacgc acatcctgcc ggaatccagc aggtaaacgg caaattcgtc
    13801 gcgcagcctg cggacttgtt ccacgctcaa tcctgtgtag ccgaacatgc cgcgctgttt
    13861 gatgaaataa gtgaaatcgc gatcggggat ttgcgcagtt aagacatcat aaagtttctg
    13921 ccgcatcgca cggatgcggt cgcgcatcat ataaacctcg ttttgccaca aggcgtaaag
    13981 ttcggggctg ttcatcacgt cggcggcgat atacgcgccg tgcgcgggcg ggctggagta
    14041 gatgcggcgg acggtgaatt tgagctgtcc gaacaccaaa tccgcttctt ccttattcgg
    14101 gcaaaccacg ctcaggccgc cgacgcgctc gccgtagagc gacaggtttt ttgagaagga
    14161 attgctgacg aacaagggca attccatttc caccgctttg cggacggcgt aggcatcgct
    14221 gtccaaatcg ccgccgaagc cttggtaggc gatgtccata aacggaatca gtttgcgcgt
    14281 tttgatgatg tgcaacactt cgtcccattg ttgttccgac atatccacgc cggtcgggtt
    14341 gtggcagcag ggatggagga tcaggacgct gttttcgggc agggtgttga aaaacgcggt
    14401 catttcgtcg aatttcacgc cgacggcagc agggtcgtaa tatgggtaag tgccgacctc
    14461 gaaacctgcg ctttcaaaaa tgccgcgatg gttgtcccaa gtcgggtcgc tgacgtaggc
    14521 gcgcgcttcg ggaaaccagc ggtgcagaaa gtccgccccg actttgagcg cgcctgagcc
    14581 gcccaaggtt tgtacggtaa caatacgtcc ttgcgcgagc gcggggttgt ctttgccgaa
    14641 caacaaatgc tgcaccgcgc tgcggtaagt gtctaagcct tccatcggca ggtagggcga
    14701 cggcacagga gtggcggcgc gggcggtttc ggcgcggctc acggattcca acacgggcat
    14761 tttgccttcg tcgtcgaaat aaatgcctat gctcaaattg actttttcgg ggcgcgggtc
    14821 gtttttgaag gtttcgacca aactcaaaat cggatcgccg ggatagtatt cgatgtgtcg
    14881 gtacatagtc cttacctctt gctttttcca aggattttct ttttcaacag cacaccgctt
    14941 tcaatatggt gcgtaaacgg aaattgatcg aacagggcgg cacgttcgac cgcatgggtt
    15001 tccgccaagg tgtccaaatt ggtacgcagc gtttcgggat tgcaggaaat gtagatgatg
    15061 ttgtcaaact gcgacaccag cttcaaagtt tcctcatcga taccggcacg cggcggatcg
    15121 acgaaaatag tggaaaatgc gtaatccgtc aaagcaatac cgccatcctt aaggcgttta
    15181 aactcacgtt ttccggtata ggcttcggta aattcttcag cagacagacg ggcgatttta
    15241 atgttgccga tgcggttggc ttcgatattc cattgcgccg cgctgacgga ggttttggag
    15301 atttcggttg ccaaaacacg ttcaaacttt tcagataaag gaagcgtaaa gttgccgttt
    15361 ccgcaataca gttcgagcaa atccccatcc aatccttctg ctgcaccgca ggcccattca
    15421 agcattttct gacacacggc ggcattcggt tgggtaaaac tgccttcaat ttgccgataa
    15481 cggaaatccc ggttgccgac cttcaaagtt tccgttacat agtcctgttt taagactatt
    15541 ttctgtcccc tgctccgccc gatgacggaa atatccaact gttgctgtaa cgcttgcgcc
    15601 gcctgcatcc actcattatc aagccttttg tggtaaatca tggtaaccag catttccccg
    15661 ctgagcgtag acaaaaattc gacggcatac caacggtttc tcagttcggc agattgggcg
    15721 gcaacggcaa tcaattctgg catcaggcgg ttgacggctt cggaagccgc ttcaaaacgg
    15781 tcgcaacgga tcaggcttgc cccgccagcc ttttgtcctt tttcaaacat ggcataaaac
    15841 atttcaccgc cttcgtgcca aatacggaac tcggcacgca tacggtaatg tttgtccgga
    15901 gattcgtaca cttcccactc aggaacatcc aaacctgcaa aaagggtttt aaggtggtct
    15961 tttttatctt gaagctgctg cgtgtagtcg ctcatatcgc tatcctgaaa aatcagaccg
    16021 gattataagc cgattcactg caaaacacgc aaccccttta ccggcatata acccgttttc
    16081 ctccaacttt tatgctaaaa tccgccgtct ccaacatacc gccgacagaa ggaaaatatg
    16141 gcaggcaaca ctttcggaca actcttcacc gtgaccacgt tcggcgaaag ccacggcgca
    16201 ggtttgggct gcatcatcga cggctgcccg cccggcttgg aattaagcga agcggatatc
    16261 caatttgacc tcgaccgacg caaacccggc accagccgcc acgttaccca acgccgcgaa
    16321 accgaccaag tagaaatcct ctccggcgta ttcaaaggca aaaccaccgg cacgcccatc
    16381 gccctcttaa tccgcaatac cgaccagcgc agcaaagact acggcaacat cgcccaaagt
    16441 ttccgccccg gccatgccga ctatacctat tggcacaaat acggcacgcg cgactaccgc
    16501 ggcggcggca ggagctccgc ccgtgaaacc gccgcccgcg ttgctgccgg agccgttgcc
    16561 aaaaaatggt tgaaagaaaa attcggcacg gaaatcaccg cctacgttac ccaagtcggc
    16621 gaaaaagaaa tccggtttga aggctgcgaa cacatttccc aaaatccttt ttttgccgcc
    16681 aaccatagcc aaattgccga gctggaaaac tatatggaca gcgtgcgcaa atcgttggat
    16741 tccgtcggcg cgaagctgca tattgaagcc gccaatgtcc ccgtcggctt gggcgaaccc
    16801 gttttcgacc gcctcgatgc cgaaatcgcc tacgcgatga tgggcatcaa cgccgtaaaa
    16861 ggcgtggaaa tcggcgcagg ttttgacagc gtaacgcaac gcggcagcga acacggcgac
    16921 gaactgaccc cgcaaggctt cctgtccaac cattcgggcg gcatcctcgg cggcatcagc
    16981 accgggcaag acatctgcgt caatatcgcc atcaaaccca ccagctccat cgccaccccg
    17041 cgccgcagta tcgacatcaa cggcaacccc atcgaactcg ccacgcacgg caggcacgac
    17101 ccctgcgtcg gcttgcgcgc cgcgccgatt gccgaagcta tgctcgcctt ggttttaatc
    17161 gaccacgccc tgcgccatcg tgcgcaaaat gcggatgttg ccgtggatac gcccgatatc
    17221 gcccgctcgg acaagtaaaa agatttagcc aaaacacggg ctttatacta aaataccgac
    17281 cattgccgtg ccgtctgaac gcacgattct tagaggaata caaccgaaat gacacaagaa
    17341 accgctttgg gtgcggcact gaaatccgcc gtccaaacta tgagcaaaaa gaaacagacc
    17401 gaaatgatcg ccgaccacat ctacggcaaa tacgatgtgt tcaaacgctt caaaccgttg
    17461 gcactcggca tcgatcagga tttgattgcc gctttgccgc aatacgatgc cgcactgatt
    17521 gcacgcgtcc tcgccaacca ctgccgccgt ccgcgctatc tgaaagcctt ggcgcgcggc
    17581 ggcaaacgtt tcgatttgaa caaccgtttc aaaggcgaag ttacccccga agaacaggcg
    17641 atcgcgcaaa accatccttt cgtgcagcag gctttgcaac agcagtccgc ccaagctgcc
    17701 gccgaaacgc cgtctgttga agccgaagca gccgaatctt ccgcagcaga ataaatcccc
    17761 aaatgaaatg ccgtctgaaa accgatttgg tttcagacgg catttttccg tatgcggcaa
    17821 tcacggttca aatatcgagt tccgccgtat cgccttcgcg ctccatccag gcacggcggg
    17881 cggcggcttc gcctttgccc atcagtttga cgaagatgtc gcgcgtctcg tcatccgcgc
    17941 cttcggggat ttgtacctgc aacaggcggc gggtgtcggg gtgcatggta gtatctttga
    18001 gctggtctgg gttcatctca cccaaacctt tgaaacggct gatggaataa gcagtttctt
    18061 tgacgccttc tttttgcagc cgctctaaaa tgccgtcaag ttcgttttgg tcgagggcgt
    18121 agaatttgcg ggcgggtttg cttttacctt gtgcgttgac atctacgcgg aacagcggcg
    18181 gctgggcgac gtagatgtgt ccgtcagcga ccagtttcgg gaagtggcgg tagaacaggg
    18241 tcagcagcaa aacttgaata tgcgagccgt ccacgtcggc atcggacagg atggcgattt
    18301 tgccgtaacg caggccgctt aaatcgggat ggtcgttgat gccgtgcgga tcgacgccga
    18361 tggcaactga aatgtcgtgg atttcggcgt tgccgaagag ctggtcgggg tggacttcaa
    18421 agctgttgag cactttgccg cgcaggggca ggatggcttg ggtggctttg tcgcgggcga
    18481 gtttggcgga gccgccggcg gaatcgcctt cgacgaggaa gagttcgttt tcgcggatgt
    18541 cttcgctttc gcagtcggtc agcttaccgg gcaggacggc gacgccgctg ccttttttct
    18601 tttcaatctt tttaaccgaa cgcatccgcg cctgtgcctg acggatggcg agttcggcga
    18661 tttttttgcc gaagtccacg ttttggttca gccacaattc caaagggtcg cccgatacgg
    18721 tagcaacgag tttcagcgcg tcgcggttgg tcagtttgtc tttggtctga ccttggaact
    18781 gcgggtcgag gacacgggca gagaggacaa aggcggtttt gccgaacacg tcgtcgcttt
    18841 gcactttcac gccgcgtggt aaaaggttgt gcagattgat gaagttgctg acggcgttga
    18901 acacggcttg tttcaagccc gcttcgtgcg tgccgcccag cggggtgggg atgaggttga
    18961 cgtagctttc gttggcgcac gagccttctt ccagccaggt cagggcaaac gcggcgcctt
    19021 cgccgatgct gaaatcgccg ttgtggccgt ctgaaatgta gttttcgcaa gagaagatag
    19081 gcacggcttc ctgcgcgtcg gcaatcaggt cggtcagata gcttttcaga ccgtcggggt
    19141 aatgccaggt ttgggtgtgc gcttcgtctt cgcctttgac cggacgggtc agggaaacgc
    19201 gcacgcccgg cagcagcacg gctttggcgc gcagcaggcg ttcgagttcg gggatgctgt
    19261 aattcgggct ttcaaaatat ttgccgtccg gccagacgcg cacttcggtg ccgctgtctt
    19321 tgacggcgca tttgcccact tgtgccaacg gttcgaccac gtcgccgccg gcaaacacga
    19381 tgcggtggat tttgccttcg cgtttgaccg ttacctcaag gcgggtggaa agggcgttgg
    19441 tcacggatac gcccacgccg tgcaggccgc ctgaaaaggc atacgcgctg ccgccgtctt
    19501 ttttgttgaa cttgccgccc gcgtgcagac gggtgaacac gagttcgact acgggtacgc
    19561 cttcttcagg gtgcagaccg acgggaatac cgcggccgtt gtcgcgtacg gaaagcgaac
    19621 cgtcttcatg aatttgcacg tcgattgccg tagcgaaacc gcccaacgcc tcgtccgccg
    19681 cgttgtcgat gacttcttgg cagatatggg tcgggctgtc ggtgcgggtg tacatgccgg
    19741 gacgttcttt gaccggctcc aagcctttga ggacggtgat gctggattcg ctgtattggt
    19801 tgtttttagc cataggaata atctgaaagt aagaaaaaca acgctttcag acggcctgaa
    19861 agcgttgcgt tccgttgttt tagcggttgt cggaagattg gcgggcggca aagtcttcat
    19921 aactttccat accgcgcagg aagcgggaag agagttcttt caacgccccc aaataaacgt
    19981 cgcgtttgaa atcaatcact tcgtcaacgg gcgcccaata ttgatgccaa cgccagccgt
    20041 caaattcggg gtggcgggtg gcgcgcaggt tgacatcgca atctcggccg gtcaggcgca
    20101 ggagatacca aatctgcttc tgcccgcgat aggatccgcg ccattcgcgg cgtacccagt
    20161 tgttcggcac gtcataacgc agccagtcgc gcgtgcggcc gataattttg acgtgttgcg
    20221 gcaaaagccc gacttcttcg tacaactcgc ggtacatggc ggtttcgggg ctttcgcccg
    20281 gcttgatgcc gccttgcgga aactgccaag aatgttcgcg cacgcgcttg ccccaaaaga
    20341 cttcgtcgcg gttgttgatt aagatgatac cgacattggg gcgatagcct tccctgtcca
    20401 acacggtgtc gccctccgtt aaattcaatc ttgggatttt cccacaaatc ggacggtttt
    20461 gacaaatcgg acgatagggt tcaggcatgt cgaaacacag acggattcgg gaaaatatct
    20521 taaatttggt ttacaataat acgtttcaaa ttaattcggg aatcagacca tgttagatat
    20581 ccaattgctc cgcagcaaca ccgccgctgt ggcggaacgc ttggcggcac gcggttacaa
    20641 gtttgatgcc gcgcgtttca atgcgctgga agagcagcga aaagccgtcc aggtgaaaac
    20701 cgaagagctg caagcctcgc gcaacagcat ttccaaacaa atcggcgcac tgaaaggtca
    20761 gggcaaacat gaagaggcgc aggcggccat ggatcaagtc gcccaaatca aaaccgattt
    20821 ggaacaggca gccgccgatt tggaagccgt gcaaaaggaa ctggacgcat ggctgttgag
    20881 cattccgaac ctgccgcatg aggacgtgcc tttcggtaag gatgaaaccg aaaacgtcga
    20941 agtccgcaaa gtcggcacgc cacgcgaatt tgactttgaa atcaaagacc atgtcgattt
    21001 gggcgaacct ttgggtttgg atttcgaagg tggcgcgaaa ctttccggcg cacgatttac
    21061 cgtgatgcgc ggacaaatcg cccgcctgca ccgcgcgctg gcgcaattca tgctcgatac
    21121 gcacacgctg aaacacggct acaccgagca ttacacaccc tatatcgtgg acgacaccac
    21181 cttgcaaggc acgggtcagc tgccgaagtt tgcagaagat ttgttccacg ttacccgtgg
    21241 cggcgacgag agcaaaacca cgcaatatct gattccgaca gctgaagtta ccctgaccaa
    21301 taccgttgcc ggcagcatta tcccgtccga acaactgccg ctgaagctga ccgcgcattc
    21361 gccctgtttc cgcagcgagg cgggttcgta cggcaaagac acgcgcggtc tgattcgcca
    21421 gcaccagttc gacaaagtgg aaatggttca aatcgttcat cccgaaaaat catacgaaac
    21481 gctggaagaa atggtcggcc atgccgaaaa catcctgaag gctttggaac tgccctaccg
    21541 cgtgattacc ctgtgtaccg gcgacatggg cttcggcgca accaaaacgt atgacttgga
    21601 agtttgggta ccggcgcaaa acacctaccg cgaaatctca agctgctcca actgcgaaga
    21661 tttccaagcc cgccgcatga aggcgcgttt caaagacgaa aacggcaaaa accgcttggt
    21721 acatactttg aacggctccg gcttggctgt cggcagaacg ctggtcgccg tattggaaaa
    21781 ccatcaaaac gccgacggca gcatcaacat ccctgccgca ctgcaaccgt atatgggcgg
    21841 cgttgccaag ttggaagtga aataagtttg caagctgcct gaacgtcaaa tgccgtctga
    21901 aacctggttc agacggcatt tcctttaaac ttttaaaaca cgtcagccgt cggcacgaac
    21961 cgcattgccg caatcgccgg tctgtccgac ctcgcggata ttggacagcg taacttccga
    22021 aatattaccc aacgcctctt ccgtcaaaaa cgcctgatga ccggtaaaca gcacgttatg
    22081 acaagacgac aaccggcgga acacgtcgtc ggtaatcaca tcgttggatt tgtcttcaaa
    22141 aaacagctcg cgctcgttct cgtacacatc catgcccaat gcgccgattt tccggcgttt
    22201 caacgcctca atcgcggcgg cactgtcaat cagcccgccc cggctggtgt tgataatcat
    22261 cacgccgtct ttcattttgt cgaacgccgc ttcgttcagc atatagtggt tttccggcgt
    22321 ggcggggcaa tgcagcgtga tgatgtccga ctgggcatac agctcgtcca aatccacata
    22381 cctgccgccg attttttccg cttcggggtt gcaaaacgga tcgtgagcca gcaggttcat
    22441 gccgaaaccc tttaaaatcc gcatggttgc aataccgatt ttccccgtgc cgataacgcc
    22501 cgccgttttg ccgtacatat tgaaaccggt cagaccttcc agcgaaaaat tcgcatcgcg
    22561 ggtacgctga taggctttgt ggatacggcg gttcaaagtc agcatcagac cgaccgtatg
    22621 ttccgcaacc gattcgggcg aataggcagg cacgcgcacg actttcaagc ccaactcttc
    22681 agccgccttt aaatccacat tattgaagcc ggcacaacgc aacgccacag ttttcacgcc
    22741 aatttgcgcc aatttttcca acacgggccg gctgccgtcg tcgtttacaa aaatacaaac
    22801 cgcttccgcg ccttccgcca ttttcgccgt tttcgcatcc agcataaaat caaaaaactc
    22861 cagctcgaag ccgaaatgcc ggttggcgcg ggtaaaatgt tcgcggtcat agcttttcgt
    22921 accgtaaatc gcaatcttca tcaatatgtc cagttgtcgt ctatggttga gaaacggcat
    22981 aatacactga attcaaacaa atcagtagaa tatggtggat taaaattgat tgcatgcacg
    23041 gcatttccat ttcaaaacac aaaactcaat cgcccattgc cgccaaaagc tccgcctgat
    23101 gttcggcaat cagggcatcg gtgatttctg ccaaatcgcc gtccatcaca aaatccagct
    23161 tgtgcagggt gaggttgatg cggtggtctg ttaccctgcc ttggggatag ttgtaggtgc
    23221 ggatgcgttc gctgcggtcg ccgctgccga tgagggattt gcgttcggcg gcttctttgg
    23281 cttgggcttc gcgtttttgc gcgtcgttca ggcgggcggc gagaactttc attgcctgcg
    23341 ctttgttggc gtgttggctg cggccgtctt ggcattcgac caccatgccg gtgggcaggt
    23401 gggtgatgcg gacggcggaa tcggttttgt tgatgtgctg accgcctgcg ccggatgcgc
    23461 ggaaggtgtc gatgcgaagg tcgacggggt tcaactcgat gtcttccagt tcgtccgctt
    23521 cgggcatgac ggcaacggtg caggcggaag tatggatgcg gccttggctt tcggtggcgg
    23581 ggacgcgctg cacgcggtgt ccgcccgatt caaatttcaa acggctgtac gccccgagtc
    23641 cgacaatacg ggcgatgact tctttatagc cgcccaactc gctttcgttg gcggaaacga
    23701 tttcaacctg ccagcggttg cgttcggcgt agcggctgta catacgcagc aaatcgccgg
    23761 caaacagcgc ggcttcgtcg ccgcccgttc cggcgcgtat ttcgatgaag atgtttttgt
    23821 cgtcgtcggc atctttgggc agcagcagtt tttgcagttc ggtatcgagc gtgttgattt
    23881 tggcttttgc cgcttcgatt tcttcggcgg caaagtcttt catttcgggg tcggacagca
    23941 tttcttcggc atctgccaag tcgctttgcg ccagttgata gttttggaat acttcgacga
    24001 cgggggtcag ttcggcgtgt tcgcgcgtga gcttgcggta gttgtccata tcggatgtgg
    24061 cttcgggttg ccctaaaagg tgggtaactt cttccaaccg gtcgccgagt tgttgcagtt
    24121 tttctaagat agacggcttc ataattcttc cataacaaac gccgcctgaa tttcagacgg
    24181 catcaaaccg cggtcattat aatatatttt ctgaatattc aaaaagataa tctagggacg
    24241 gagcaggctg tcggcattgc gttccgttat cgcggcgatt tccgccgcgc cggtaccgcg
    24301 tatctgcgcg gcaatttcgg caatccgaag aatgttggca ggcgtattga cggtgttttt
    24361 caacataaac gggctatccg tttccaatac gaaatccccg tcgttcaagg ctttaagcgt
    24421 atcgcgcact ttacgcgcgt tcgggttgag cagcagcgaa ccgatgccga ttttgaaacc
    24481 cagtttcgcc aacacgtgcg cttcttcaat actgccggaa aaggcgtgga caatgccgcc
    24541 ccaggtaaag cccgtccgtt tgacggcggc ggcgatgtcg gcggcggctt tgagattatg
    24601 gataatcacg cgccgacgca gggtttgcgc gatttcaagc tggcggctga agacttggat
    24661 ttgccgttcg cgctgctgcg gcgtttgggt tttgtcgtaa aaatccaagc cgatttcgcc
    24721 gacccatgcc tgcggataac gtgccaacat cgcttccagg cggacgcaat cacgctcggc
    24781 aatgccgtct gaaaaccaag gatggatgcc gagcgcaata cggatttgac cgtgttcgga
    24841 cggcatttcc gacagttccg ccacgctttg ccaatcctgc ggacaggtgg cgggaacgat
    24901 aaaccgcttc accccaactt tccgcgcggc ggtcaggatg tgcggcaggt tttcgcgcag
    24961 ggcggcatcg gcaaggtggc agtgggtgtc ggtgaagttc atttcgattc cgcaatgcgg
    25021 caggttcaca taaaaataag gcagggcggc aagggatgcc gtgtacgggc ggcgcataag
    25081 gcgcggtcaa cgccccgtca ttgccatttc aatccgctat aacttcagtc ttgccaactc
    25141 tttttctaca tcttccgcgc cccagccttg cgatacggcg agggtcatca gcgcggtggc
    25201 ggtttcgagg ttgcatttgc cgccgttgat gatgcccgag ttgcggaacg cattgccttg
    25261 cgcgtaaacg gcggcagttc tgccttgtcg gacttggctg atgttgagca acaattttcc
    25321 ttgccgcgtg aagtctcgga cagcgcggat aaagccttcg tctgcgggcg tgttgccgtg
    25381 tccgtaactt tgcaggataa gggcgtgggc gggaagctgt ccgagtccgt cggcaagttc
    25441 ttggacggca aagccgggga taagcgtgcg gacggcgatt tttgcctgcg ggtcgggata
    25501 acggattttg aggccgtctg aaacatctgc cgtgtcctga ttcgggatgc ggacattgtg
    25561 ccagccctgg gtttcgtccc actccgccag tgcgccgaaa tgcggattgt cgaaaccggc
    25621 ggcggtttcg gtgctgactt tgctgctgcc gacggcggga taaagtttgc cgtcaaaggc
    25681 aataagcgtc tgcttcaatc cgagtgggaa tgcggcaacg gcagtcgcca gattgcgcgg
    25741 cgcgtcgctg cccacggcgt tgtacggcca ttgggagccg gtcaggacga ttggcttgtc
    25801 caaaccctgc agcgcgaggg cgaacagatt ggcggtatag gcgagcgtgt ccgtgccgtg
    25861 cagcaccaaa acgccgtcgt actgcggcag cgcggcggca atgatgtcca gccaagcgca
    25921 ccagttttgc agttggacgg cggaagaatc aatcagcgtc ccacaaacgt gccaatcgaa
    25981 atccatgccg tttgaaaacg gcgcgagtgc ctgcccgacc agcgcggtat cggggcgcag
    26041 gccttcgctg ctttgcgtca tgccgatggt accgccggta taaaggacaa aaattttctg
    26101 tttcatggct catcctgaaa atgcaaagac cgcctgaaac gcgaaatgcg tccggacggt
    26161 cgaaaatctg atttactgct gcttctgtgc cgcttttttc gctgcctttt ccgccttgcg
    26221 tttggcgcgt ttttcgctca attgtgcgcg gtaaagctga taatgtcggc gtttgcgcca
    26281 ccagaaccaa gccagcgccg ccgccagcac gcccaatgcg atgaagatgc cggattgcag
    26341 gctgtgcatt ttcgccatca gccaatcgat gttgtgcgcg ccgtactcgc ccaagtaaat
    26401 ccaaacgggc acggaaatca gcgcggcaag cccgtccata atcagaaagc gcagatacga
    26461 tactttgcgg ctgatgccgg cggtaacgaa aacggcagtc cgcaaaccgg gcaggaaacg
    26521 agcgacaaat aacacccagt tgccgtattt gtcgaatttt tcctgaacct gtgcgtaacg
    26581 tttcggcgtc atgatgcgcg caatcggttt gaacttgagg attttctgcc cccaaattcg
    26641 tccggcggcg aacatgatgc cgtcgcccgc caacacgccg agcatgccga ctgcaaacat
    26701 aatatgcgga ttggtataac ccatacccga aatcacgccg cctgttacca aggtcaaatc
    26761 ctcgggaatc ggcacgccga aaccgcagat gaccaaaacg aaaaacacgg ccgcgtagcc
    26821 gtattcgaca aaaaaggctt ccaaaagggc aaacatagcg gatattccat tgtcggagat
    26881 aaaaagtcag aacaagccga aacattttct gcgcgaaacg gcattctatc aaagattatg
    26941 ccgtctgaaa gcggcaaggg gcagattcgc gccctctgcc ggacgttcag acggcattta
    27001 aacaggaaaa ccgccgctgt tgaaacggtt ttgcctgatt ttgcctaaag gctgccgatg
    27061 gcggaagcaa tacgttccgc accgtcgcgc gcccaatccg cctgccgcgc ctccaccatc
    27121 acacgcacga cgggttcggt tcccgaagcg cgcaacacga cacgcccttt gccttcgagt
    27181 tctttttcca cttccgccaa cacgtctttt gaggcttcct gccagttttg ccctttgccg
    27241 atacggacat taatcatcgt ttgcggatac ggctgccaat cggcgcaaac ggtggcgagg
    27301 tcttggttca gcgtttgcag tgccgccaaa acttgcagtg cggaaataat gccgtcgccg
    27361 gtgttgtgtt tgtccataca caaaatatgg ccgctggctt cgccgccgat gagccagctg
    27421 cgttggttca gctgttccaa cacatagcgg tcgccgactt tggcgcggca gaaatcgacg
    27481 ccctgctctt tcagggcgat ttccatcgcc atattggtca tgaccgtgcc gaccacgccg
    27541 ccgatgttga taccttctcg ggcgcgggct ttggcgatga cgtaaatcag gctgtcgccg
    27601 tcgtaaacct taccgttttt atcgaccatc atcaggcggt cgccgtcgcc gtccaaggcg
    27661 atgccgtagt ctgcctcgtt ttgcaacacg gcggcctgta gggttttggt atgggtcgcg
    27721 ccgcattttt cattgatatt gtagccgtcg ggttcgtcgc cgatgctgac gacctgcgcg
    27781 cccagttcgt gaaacacttt gggtgcgacg ctgtaagccg cgccgttggc ggcatcgacg
    27841 accagcttca gcccgcgcaa atcggaatgc gtagggaagg tggatttgca aaattcgata
    27901 tagcggtcgt ccgcaccgct gatgcggcgt gcgcgcccga ggcgtgcaga aggctgggtt
    27961 ttcatttctt cgtcgatttt ggcttcgatt tccaactcga tttcatcgct cagtttcacg
    28021 ccgccctcgg caaagaattt gatgccgttg tcggaatagg cattatgcga cgcggaaatc
    28081 atcacaccgg cggacaaacg cagcgcacgg gtcagataag ccacgccggg cgtaggcagc
    28141 ggaccggttt ggataacgtt gacacctgcg gcggtaaaac ctgccaccaa agcggcttcc
    28201 agcatataac cggaaatacg cgtatccttg ccgatcaaaa cggtcggttt ctggtcggtg
    28261 tcgtgctgaa ccaaaacctg ccccgccgca tagccgagtt tcaatacaaa atcgggcgta
    28321 atcggaaact gcccgacttc gccgcgcacg ccgtccgtgc cgaaatattt ttttgccatg
    28381 tgttgctccg agaatgtgaa ccgttgtccg agattataca gtctgtttgt accttgctgt
    28441 ctgcaccgtt gatgccgtct gaagccgccc cgtccttttc agacggcatg aagtatgtga
    28501 accgctgttt acagattgat gcccaacgct tcccacacct tcaaagcatc cgccgttgcc
    28561 ttcacatcat gcacgcgcac gatttgtgcg ccgcgcgcta cggaagccaa cgctgccgcc
    28621 acgctgccgt gtacgcgttc cgccgcattt gcctcgccgg tcagctcgcc tatcgtgctt
    28681 ttgcgcgata cgccgatgag cagcggaaaa cctgtttccg ccatcaattc gggtaaatgc
    28741 cgcatcagcg cgatattgtg ttgcaaggtt ttgccgaagc cggagccgaa gccggggtcg
    28801 agtatgatgc gttgcggtgc gatgcctgcc gcgatacatt ccgctgagcg cgctttcaaa
    28861 taccgcgcta cttcaccgac aacatcttga tatttcggat taatctgcat ggttttgggc
    28921 aaaccctgca tgtgcatcag gcaaatgccc gtgtccgcct gacgcgccag caattcgacc
    28981 gcgccttcgt cggtcaacgc cgccacatcg ttaataatat cgatgccgcc gagtgccaac
    29041 gctttttcca taaccaccgt gcggcgcgtg tccaaactaa cgggaacgcc ccaccccgcc
    29101 acttctgcca atacaggctc aacccgcgcc cattcttctt caggcgaaac ataatccgca
    29161 cccagccgcg tcgattcgcc gccgatgtcg agaatgtccg caccctcttt caaaagccgt
    29221 tcggcatgcg ccaaagctgt ttgggcgttt tgcgaatacg ctccgccgtc ggaaaaagaa
    29281 tcgggcgtga gattcacgat acccatgatt ttcggtttgt ccaaaccgat ttcaaaccgt
    29341 cctgcctgcc aaacgtgtcg tgccatctga actcctccca aaataaaaaa cagattgaga
    29401 cctttgcaaa aatagtctgt taacgaaatt tgacgcataa aaatgcgcca aaaaattttc
    29461 aattgcctaa aaccttccta atattgagca aaaagtagga aaaatcagaa aagttttgca
    29521 ttttgaaaat gagattgagc ataaaatttt agtaacctat gttattgcaa aggtctcaga
    29581 ttatatgccg tctgaaaccg tcttgtgcgc ttcagacggc accgctattc gggcggcaga
    29641 cggcatgttg tccgaatgtc tgctccgcct ttgaatctgc cggtatgcct gctatccgcc
    29701 cgacttttca aaacaggttc cgacgattcc gcacgcgcct gccgcctttg ccaagccgta
    29761 caggatttcc tgcggcatat cgcggttcca taatcccgta atattcgcaa tcacgggcag
    29821 atggctgatt tggcggactt tcacgatgga ttcgacatcc aaacggtagg gatggccttt
    29881 ggtatggttc aatacgcccg actcgcccag aatcaggtgg cggttgccgc gcgagacgac
    29941 atattctgcg gcattcaacc aatcttcggc agaatggtgc ttgtctttac acagcaccaa
    30001 gggaatgttc aggtttccgg cttcattcaa tacggcaaga tcggacatca gcccgccgcc
    30061 caaatacagg atatccgccc ccgcattcaa agccgcttcg acatggcgga cgttgcggac
    30121 gcgcaccaat acgggtttcc ctgcatcatg cgccgatgcg gcctgttccg ccaaccgtct
    30181 gcaccgtccc cgcccttcat ccgcacttga agtgtcgtat aagtttgccg aagtgaaaaa
    30241 cggatccaga aacactgcat ccgcattgcc ccatactgac ggttctgcgg cgatacggac
    30301 ggtttccccg ccgccgaaag ccacgccttt ggcggcaacg cggctgtctt ccgcccgatt
    30361 ttcccgactg acggttttcc atgtatccaa aatgcggacg gctttctcga cctccggcag
    30421 cgtctgcacc tccctgacgc tcaaaaccct atcgtcgccg attgcgccga tgacagtacg
    30481 ctcgtcgccg tgagaaatgt gttctcgcag acctctgctg cggataaagg cgacaacgcc
    30541 ggcaatgtcc gcttcggcgg cacgcctgct catgacaata atcatatttc ctcctgacac
    30601 aagaaacggc ctgcccaaaa taggattttt gcaagccgtg ttatactgtg gcgtgtttta
    30661 cagattgttc gggctatgga tttattatcg gttttccaca aataccgtct gaaatatgcg
    30721 gtggccgtgc tgacgatact gcttttggcg gcagtcgggc tgcacgcttc cgtatatcgc
    30781 accttcacgc ctgaaaacat ccgcagccgc ctccaacaaa gcattgcaca cacacaccgg
    30841 aaaatctcgt ttgatgcgga cattcagcgc aggctcctgc cccggccgac cgtcatcctg
    30901 aaaaacctga ccattaccga acccggcggc gaccggactg ccgtttccgt ccaagaaacc
    30961 aaaatcggat tgagctggaa aaacctgtgg tcggatcaga tacagattga aaaatgggtg
    31021 gtttcgagtg cggaacttgc cctgacgcgc gacgggaaag gtgtttggaa cattcaagac
    31081 ctgatcgaca gccaaaaacg ccaagcctca gtcaaccgca ttatcgtcga aaacagcacc
    31141 gtccgcctca atttcctgca ggaacagctt atcctgaagg aaatcaacct caacctgcaa
    31201 tcccccgatt cgtcggggca gccgtttgaa agttcgggca tactggtttg gggaaagctg
    31261 tccgtcccgt ggaaaagcag ggggctgttc ctttcaaacg gcatcggccc gcccgaaatc
    31321 tcaccgttcc attttgaagc ttccacttcg ctggacggac acggcattac catttccacc
    31381 accggcagcc cttctgtccg cttcaacgcc ggcggagcgg atgccgccgg cctcggcctg
    31441 cgtgcagaca cttccttccg taacctccac ctgaccgccc aaatccctac gctggcactc
    31501 aggaacaaca gcattaaaat tgaaaccgtc aacggcgcat ttaccgccgg cggcgaatat
    31561 gcccaatggg acggttcgtt caaactcgac aaagccaacc tgcactccgg catcgccaac
    31621 atcggcaacg ccgaaatctc cggcagcttc aaaacaccgc gccaccagac caacttctcc
    31681 ctcaattcgc cgctcgtatg gacggaaaac aaagggctgg acgcgccgcg cctgtatgta
    31741 tcgacccttc aggataccgt caaccgcctg ccgcaacccc gtttcatcag ccggctcgac
    31801 ggttcgctgt ccgtaccgaa tctgcaaaat tggaatgccg aattaaacgg cacattcgac
    31861 cgccaaaccg ttgccgcgaa attcagatac acacatgaag acgcaccgca tctggaagcc
    31921 gccgtcgcac tgcaaaaatt gaacctgacc ccctatcttg acgacgtgcg gcaacaaaac
    31981 ggcaaaatat ttcccgacac cctcgccaag ctgtccggcg acatcgaggc gcacctgaaa
    32041 atcggaaaag tccaacttcc cggcctgcaa ctggacgata tggaaaccta cctccacgcc
    32101 gacaaagacc atatcgcgct cagccgtttc aagtcagggc tttacggcgg ccataccgaa
    32161 ggcggcatca gcatcgccaa cacccgtccc gccacttacc gcctgcaaca gaatgcaagc
    32221 aacatccaaa tccaaccgct gctgcaagac ctgttcggct tccacagctt cagcggcaac
    32281 ggcgacgcgg tcatcgacct gaccgcgggc ggcgaaaccc gaaaagagct tatccgctcg
    32341 cttcaaggca gcctgtcgct aaatatttcc aacggtgcgt ggcacggtat cgacatggac
    32401 aatatcctga aaaacggcat ttcgggcaaa actgccgaca atgccgcacc cagcacaccc
    32461 ttccaccgat tcacgctcaa cagcgaaatt tcagacggca tcagccgcca catcgatacc
    32521 gaactcttct ccgacagcct ctatgttacc agcaacggct ataccaatct ggatacgcag
    32581 gaattgtctg aagatgtcct tatccgcaac gccgtccatc cgaaaaacaa accgattccc
    32641 ctgaaaatca ccggtacggt ggacaagccg tccattaccg tcgattacgg caggctgacc
    32701 ggcggcatca attcgcgcaa agagaaacag aaaatcctcg aagacaccct gctggaacaa
    32761 tggcagtggc tcaaacctaa agaaccgtaa acatcctgcg tacacaaatg ccgtctgaaa
    32821 cacccccacg cttcagacgg catgaccgta aaacctacaa ccccaattcc tcccaaatcc
    32881 catcaatctt agccgtaacc gcagggtctt ttttgatgac gcgtccccat tcgcggtcgg
    32941 tttctcccgg ccatttgttg gtcgcatcca aacccatttt gccgccgagt ccgctgacgg
    33001 ggctggcgaa gtcgagataa tcgatgggcg tgttttctac caaaacagtg tcgcgcacgg
    33061 ggtccatgcg cgtggtgacc gcccagatga cttctttcca gtcgcgcacg ttcacatcgt
    33121 catccaccac gatgatgaat ttggtataca taaactggcg caggaacgac cagcagccca
    33181 tcatcacgcg cttggcgtgt ccggcgtact gttttttcat gctcaccacc gccatgcggt
    33241 aggagcagcc ttcgggcggc aggtagaaat cggtgatttc gggaaactgc ttttgcaaga
    33301 gcggtacgaa cacttcgttc aatgccacgc ccaaaacggc aggttcatcg ggcggtttgc
    33361 ctgtgtaggt cgaatggtaa atcgggtttt cgcgcatggt gatgcgttcg accgtaaaca
    33421 tggggaaatg gtcctgctcg ttgtaatagc cggtgtggtc gccgtatggg ccttccagcg
    33481 cggtttcgtt cggatgaatc acgccttcca acacaatttc ggcgcgtgca ggcacttgca
    33541 aatcgttgcc gatacatttc accagctccg tccgcgaacc gcgcagcagt ccggcaaact
    33601 ggtattcgct caaggtatcg ggaacaggcg ttaccgcgcc caaaatggtg gcggggtcgc
    33661 agccgagtac gacggcgacg ggatacggcg tatcgggatt gagtttgtgg aattcctgat
    33721 aatccaacgc gccgccgcga tgcgacagcc agcgcataat cagcttgttt ttgccgatga
    33781 gttgttggcg gtaaatgccg agattttggc gttttttgtg cggcccgcgc gtgacggtca
    33841 agccccatgt taccagcggc gcaacgtctt ccggccagca atgctgaatc ggaagttgat
    33901 acaaatcaac gtcttcgcct tcccacacga tttcctgaca cggcgcgttt ttcaccacgt
    33961 tcggcaccat gctccaaatg tctttcaaga gcggcagttt ggaaaacgcg tctttgatgc
    34021 ctttgggcgg ttcgggttct ttcaaatacg ccagcgtctg cccgatttcg cgcagcttgg
    34081 acacgctgtc cgcacccatg cccattgcca cacgttcggg cgtgccgaac aggtttgcca
    34141 acacgggata atcgtagcgc gtaccgtcgg gcttaactgg gttttcaaac aacaatgccg
    34201 gcccttcggc gcgcagcacg cggtcggcga tttcagtcat ttccaaatgc ggggaaacag
    34261 gatgcgcgac gcgcttgagt ttgccctgct gctcgagcat agcgatgaag tcgcgcaggt
    34321 ctttgtattt catatttatc ctttttgtcc ttttatcctg aacaatccaa ttcggatacc
    34381 gcccctatcc ttgcctgcgc ttcggcatat tctatgccgt gataaaagtc gcgtaccagc
    34441 ggatgttcgc cgccttgatg gagttgcaac aaaggacgtt gaccatcggg ttgggtaacg
    34501 acattgcagt gcagaccgaa ggtgtcggtt tcataagggg gcagctggtt gcagatcatg
    34561 ccgaaataaa cagcgttttc aaggttgtcg taaaagcggc tttgatagtc gttaaaactc
    34621 ttttcgctga cggataccca cacgccgtat tccagtgttt cctgatgccc gataatcgga
    34681 atcggcagca ccgcgcggat aaagcggtcg gtttggtcgg tatagcggat gatgcagaaa
    34741 tcagaatcac attccgcttg ataagcaatg cgttcttctt cactgagttg attataggga
    34801 tagggggcgg taaagccgat tgcgggcatt tcttcgtggt tttcgccgca ggaagtgcaa
    34861 gtgtacataa ggtttcagac tttcaaaacg agtttgcggt aaagccattc gccggcaaac
    34921 agcatcccca tcaatacata agcaatcacg cctgtataaa ccgcccacca atcataccgc
    34981 cccaaccgtg ccaacaaagc ggcaagcgtc ccgttgatta taaaaaatac gcaccaaagc
    35041 tgcgttaccc ggcgggtata gcgcacggct ttttcaggca ggtcgggctg ttgcagccgc
    35101 gcaagttttt caatcaccgt ctgcccggca aacaggctgc cgccgaacac cgccaacatc
    35161 atcagattga cgaggacggg ataccaatac atcgaatcat gccgcccgaa caccaatact
    35221 gcggcaaaaa acagtgtaat aaacaaagcc gcataacgct gttggggcag tttggcagtc
    35281 agggcgcgca gcagccacaa cccgcacatc gccgccgcca gccagacaaa ccagcccgcc
    35341 tccctgccgt aatatagtgg attaacaaaa accagtacag cgttgcctcg ccttgccgta
    35401 ctatctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactatacc acaaagcggg
    35461 ataggcaatg cttaatacgg tcagaaaaat atgtccgaaa aaaccgggtt tcattttgaa
    35521 tccgcacaaa tgttttcaga cggcatccga taaaaacatg ccgtctgaaa aataattaga
    35581 aatacccgat tagcccgcct gaatcttcaa taccgcctgt accacgtcgt tgacggtgcg
    35641 gacattgcgg aaatcttcag cctgcagctt gcgggctgcc acagccattc acgattgcga
    35701 tatacccggc ggcattcccg actgccgttc agcagaaagc gcacccattc caaaccgctc
    35761 caatatgcct cgggcagcat accggcttca gacggcatat cgtccgaagc agataaagtc
    35821 aggctgtaac gcgtcccttt ggtcagaacc atcgccaaag cataagcaaa cggcgcgcgc
    35881 gttgccgata cggcatatcc ttccggcagc ggatcgtccg ccgccaaaac caaaaccgac
    35941 ccgcatccct cttccaacag tgatgccgct tccgccaatg ccgtttccac accgtcggca
    36001 cacacggcca atgccgtctg ctcgctcata tcccgcctca atatcgacca ttaccccgcc
    36061 gttgcattgt gcaccgacaa accgaacgaa gtcggcgaca cgatttgcga tttcaacagt
    36121 tccaaccaca aatcgaaact gcgcgccatt tccccgtcgt gcgaggcata aactatagtg
    36181 gattaaattt tactagatta gcagatatgt taccctcgaa atatgaagat aacacactgc
    36241 aaattaaaga aaaaagtaca gaaagaactg ctccattttt ttgtactgga agttaccgcc
    36301 cgttctgccg ccgatatttt gggtatccat cccaattcgg cagcactgtt ctaccgtaaa
    36361 atccgcacgg ttatcaacca tcatttggtc ttggctgccg atgaggtttt tgaggatcct
    36421 gtcgagccgg acgaaagcga tttcggcgga cggcgtaaag gcagacgtgg tcgcggtgcg
    36481 gcaggaaaag tggttgtttt cggcattctg aaacgcaacg gacgggtcta taccgttgtc
    36541 gtaaacaatg ccaagtctga aacgttactc cctgtcatca aaaagaaaat catgccggac
    36601 agtattgttt ataccgatag tctgagcagc tgcgacaagt tggacgtgag cggttttatc
    36661 cattaccgca tcaaccattc caaggagttt gcagaccgtc agaaccacat taacggcatt
    36721 gagaactttt ggaatcaggc aaaacgcgtc ttgcgaaaat atagtggatt ggcacgaacg
    36781 tttccccgaa ttggaagcgc aactgggtgc gccgattgag gattacgcgc cgccgaaaca
    36841 ttggacgcgc aagcagctca gaagtatggg gcgtgtgtcg tatttgtgcg tcgatgcggc
    36901 ggagcaggct ttgacggatg ccggtttgct cggggacgaa agcattaccg acggacggat
    36961 gggcgttgcc tgcggctctt ccagcggcag caccaaagac atcggcgatg taggcgaatt
    37021 gttgctgacc ggcatgtcgc gcaacttcag tgccaacacc tatgtgcgta tgatgccgta
    37081 caccgccgcc gccaatatcg gcatcttttt cggactgaaa ggacgcatca tcccgacatc
    37141 gagcgcgtgt tcgtccggca gccaaggcat aggttatgcc tacgaagcca tcaaatacgg
    37201 tctgaccgat atgatgctgg cgggcggagg cgaagaattt ttcccgtccg aagtgtatgt
    37261 tttcgactcg ctttatgccg ccagccgccg caacggcgaa ccggaaaaaa ccccgcgccc
    37321 atacgacgcg aaccgcgacg ggctggtcat cggcgaaggc gcggggattt tcgtgctgga
    37381 agaattggaa cacgccaaac ggcgcggtgc gataatttac gccgaactcg tcggctacgg
    37441 agccaacagc gatgcctacc atatttccac gccccgcccc gacgcgcaag gcgcaatcct
    37501 tgcctttcag acggcattgc aacacgcagg tcttgcgccc gaagacatcg gctggattaa
    37561 tctgcacggc accgggacgc accacaacga cagtatggaa agccgtgccg ttgcagcggt
    37621 tttcggcaac aatacgccct gcacgtccac caagccgcaa accggacaca cgctgggtgc
    37681 ggcgggcgca atcgaagccg cgttcgcgtg gggctttgcc gaccggcaaa gcaatcccga
    37741 aggaaaactt ccgccccagc tttgggacgg gcagaacgac cccgacctgc ccgccattaa
    37801 cctgaccggc agcggcagct tctagacaac ggaaaaacac attgccgcca gctcgtcgtt
    37861 tgccttcgga ggaagcaact gcgtcttaat catcggatga aataagtttg tcaatcccac
    37921 cactatgcta tacaatacgc gcctactctt gacgggtctg tagctcaggg gttagagcag
    37981 gggactcata atcccttggt cgtgggttcg aaccccaccg gacccaccaa ttcccaagcc
    38041 cggacgtatg tttgggcttt tttgccgccc tgtgaaacca aaatgctttg agaaaccttg
    38101 ataatgaaaa aagtcagcgt attgattgtt gccaaaaacg aagcaaacca cattcaggaa
    38161 tgtattgaaa gttgccgttt cgataaagaa gtcatcgtta tcgacgacta cagcaccgac
    38221 aatactgccg aaattgccga gggtttgggc gcaaaagtct tcagacggca tttgaatggg
    38281 gatttcggag cgcaaaaaac atttgccatc gaacaggcag gcggagaatg ggttttcctg
    38341 attgatgcag acgaacgctg cacgccggaa ctatctgatg aaatctcaaa aattgtccaa
    38401 accggcgatt atgccgccta ttttgtcgaa cgccgcaacc ttttccccaa ccatcccgcc
    38461 acacacggcg cgatgcgtcc cgacagcgta tgccgtctga tgccgaaaaa agacagttcg
    38521 gtgcaaggca aagtacacga aaccgtacaa accccctacc ccaaacgccg tctgaagcat
    38581 tttatgtacc attacacgta cgacaactgg gaacaatatt tcaacaagtt caacaaatat
    38641 acttccattt cagccaaaaa ataccgagag cagggaaagc ccgtgcgttt cgttagggac
    38701 attatcctcc gcccgatttg ggggtttttc aaaatttata tcctgaacaa agggtttctt
    38761 gatggaaaaa tgggttggat tatgtccgtc aaccacagct attacacgat gattaaatat
    38821 gtcaaactat attatctgta caaatccggc ggaaaatttt aaatggaaaa agaattcagg
    38881 atattaaata tcgtatcggc caagatttgg ggtggaggcg aacaatatgt ctatgatgtt
    38941 tcaaaagcat tggggcttcg ggactgcaca atgtttaccg ccgtcaataa aaataatgaa
    39001 ttgatgcaca ggcgattttc cgaagtttct tccgttttca caacgcgcct tcacacgctc
    39061 aacgggctgt tttcgctcta cgcacttacc cgctttatcc ggaaaaaccg catttcccac
    39121 ctgatgatac acaccggcaa aattgccgcc ttatccatac ttttgaaaaa actgaccggg
    39181 gtgcgcctga tatttgtcaa acataatgtt gtcgccaaca aaactgattt ttatcaccgc
    39241 ctgatacaga aaaacaccga ccgctttatt tgcgtttccc gtctggttta cgatgtgcaa
    39301 accgccgaca atccctttaa agaaaaatac cggattgttc ataacggtat cgataccgat
    39361 cgcttccctc cctctcaaga aaaacccgac agccgttttt ttaccgtcgc ctacgccggc
    39421 aggatcagtc cggaaaaagg attggaaaac ctgattgaag cctgtgtgat actgcatcgg
    39481 aaatatcctc aaatcaggct caaattggca ggggacggac atccggatta tatgtgccgc
    39541 ctgaagcggg acgtatctgc ttcaggagca gaaccatttg tttcttttga agggtttacc
    39601 gaaaaacttg cttcgtttta ccgccaaagc gatgtcgtgg ttttgcccag cctcgtcccg
    39661 gaggcattcg gtttgtcatt atgcgaggcg atgtactgcc gaacggcggt gatttccaat
    39721 actttggggg cgcaaaagga aattgtcgaa catcatcaat cggggattct gctggatagg
    39781 cttacgcctg aatctttggc ggacgaaatc gaacgcctcg tcttaaaccc cgaagcaaaa
    39841 aacgcactgg caacggcagc tcatcaatgc gtcgccgccc gttttaccat caaccatacc
    39901 gccgacaaat tattggatgc aatataaact gctttcagac ggcatatgcc gtctgaaagc
    39961 ctttgatgca acaaaccgcc aaattatatt cgttcattgg aaagaaacac cccgaatcca
    40021 tccttaaaaa taagaaaatc ccaatatccc ccgatattac gcagcctatt ggcaaagttt
    40081 tgcagcgtct tccccggctt gtgctgccgc atcaagtgct ttgttacaat gtatagtaga
    40141 ctaacaaaaa ccagtacagc gttgcctcgc cttagctcaa agagaacgat tctctaaggt
    40201 gctcaagcac caagtgaatc ggttccgtac tatctgtact gtctgcggct tcgtcgcctt
    40261 gtcctgattt ttgttaatcc actatactat cttcacattt cttaataaat tttatgagta
    40321 accatacttc ttggtcgtcc aaaatcggtt tcgtgcttgc tgcggcgggt tcggccatcg
    40381 gtttgggcgc gatttggaaa ttcccttata cggcgggcac caacggcggc gcggtgtttt
    40441 tcctgctgtt tttgatattt actatcttgg tcgccctgcc cgttcagctt gccgaatttt
    40501 atatcgggcg cacgggcggt aaaaatgccg tcgattcctt cagggctctg cgtccgggta
    40561 cgcaatggct ttgggtcggg cgcatgggcg ttgccgcctg ctttattttg ctgtcgtttt
    40621 acagcgtggt cggcggatgg gtattgaatt atgtcgtcca cagtttcacg ggggcggttc
    40681 ataccggcgc ggactttgaa gccttgttcg gcgcgacgat ttccaatccg gcaggttcgc
    40741 tgtcctatca ggcactgttt atgctgatta cggtttgggt ggtcaaaggc ggcatttcag
    40801 acggcattga aaaggcaaac cgttatctga tgccggggct gtttatcctc tttattgcgc
    40861 tggcaatccg ttcgctgacg ctgccgggtg caatggaggg cgtgtctttc ctgctcaaac
    40921 cgaattggtc gtactttaaa gccgatacga tgattacggc tttaggccag gcgttttttg
    40981 ccctgagcat cggcgtttcc gccatgatta cctacgcttc atatttggga aaagatcagg
    41041 atatgttccg ttccggccat acgattatgt ggatgaacct cttggtttcg ctgcttgccg
    41101 gcctggtgat ttttccggcg gtgttcgcct tcggttttga accgagccag gggccgggat
    41161 tgatttttat cgtattgccc gcagtgttta tgaagatgcc gttcggtacg gttttgtttg
    41221 cggtatttat gctgctggtc gttttcgcca cgctgacttc ggcattttcg atgttggaaa
    41281 cggtcattgc ctcaaccatc cgccaagacg agcgcaaacg caaaaaacac acttggctta
    41341 tcggcacggc cattttcatt atcggcatcc cgtccgcgct gtctttcggc gtatggggcg
    41401 agtttaaggt tttcggcaaa accatttttg atttgtggga ctatgttatt tccgccgtca
    41461 ttatgccgat tggtgctttg agtgtttcca tctttaccgc ctggattcag gacaagcagt
    41521 ctgtgttaaa agatgccggc gcgggcagca ccgtaccacg ggcagtgctg ctgctgtggc
    41581 tgaatacctt gcgctacctt gccccgattg ccattattat tgttttcatc aattctttgg
    41641 acatccttta aaagccatcc aaacagcaaa aatgccgtct gaaagccttt cagacggcat
    41701 ttttgcttcg ggttcagcct atttcgtt
//
LOCUS       DAWFHY010000007        39626 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.007, whole genome shotgun sequence.
ACCESSION   DAWFHY010000007 DAWFHY010000000
VERSION     DAWFHY010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 39626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39626
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.007"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..111
                     /locus_tag="WGQ68_000300"
     CDS             <1..111
                     /locus_tag="WGQ68_000300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0714179.1"
                     /translation="RAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            276..1113
                     /locus_tag="WGQ68_000301"
                     /pseudo
     CDS             276..1113
                     /locus_tag="WGQ68_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248023.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1215..2090
                     /locus_tag="WGQ68_000302"
     CDS             1215..2090
                     /locus_tag="WGQ68_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0714180.1"
                     /translation="MFGFRLSGGKGILQRSQVALVVTESTGGLEIPAAKAIRRAGIAV
                     IIANPRQTHQFAQSQPLTKTDAKDAKMLAFFAQMMTQKEDWQTMPYQPPTEAEEVLEA
                     LVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHFDRLIDELDKQIDDHTHTHFDG
                     KAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASLAGIAPHPRESEETKFKSRCFG
                     GRSAVRKALYMATVVATRFKPLIRDFYQRLPSEGKPYKVAVTAYMRKLLTISNARMRD
                     YFAENDTAENGIRTA"
     gene            2138..3211
                     /locus_tag="WGQ68_000303"
     CDS             2138..3211
                     /locus_tag="WGQ68_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0714181.1"
                     /translation="MRPFFVGAAVLAILGALVFFISPGAIVLHRQIFLELMLPAAYGG
                     FLTAALLDWTGFSGNLKPAATLMAVLLLVAAVLLPFLPQLAAFFVAAYWLVLLLFCAR
                     LIWLDRNTDNFALLMLLAAFTVFQTAYAATGDLNLLRTQVHLNMAAVMFVSIRVSVLM
                     GAEALKECRLKDPVFIPNVVYKNIAITFLLLHAAAELWLPTQTAGFTALAVGFILLAK
                     LRELHHHELLRKHYVRTYYLLQLFAAAGYLWTGAAKLQNLPASAPLHLITLGGMMGGV
                     MMVWLTAGLWHSGFTKLDYPKLCRIAVPILFAAAVSRAVLMNVNPIFFITLPAILTAA
                     VFVLYLFTFVPIFRANAFTGDPE"
     gene            complement(3257..5446)
                     /locus_tag="WGQ68_000304"
     CDS             complement(3257..5446)
                     /locus_tag="WGQ68_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEZ0714182.1"
                     /translation="MIYHRIAVNVPLSDGLLTYSHSDPLPPGTRVLVPFRNKTVVGMV
                     WETDIAPDMDTARILGVQTTFSDEPLLPESWRDLLAFTSRYYHYPTGQAVFAALPQGL
                     KETRAVEMPQPPLFYALNEAGRAQTPPPARFNKKAALWDALLSGEMTMAALKQVNAQA
                     AKLIEDWAVQGWIETTEAAKPVLRPYHGQASHSEFVLNANQQKASDAIQTAFGSFQPF
                     LLYGITGSGKTEVYFDAMAKVLAQGRQVLFLLPEINLTPQLLKRVENRFADVPTAVLH
                     SQMAAGKRTQDYLRAMLGQAKLVIGTRLAVFTPMDDVGLIVVDEEHDGSFKQDNELRY
                     HARDLAVWRAKQSGCPVVLGSATPSLESWHKAQSGAYRLLQLTERAHTAAQLPQVDIL
                     NVGRLKLDNGFSPQALQLLKQNFEAGGMSLVYLNRRGFAPALFCGDCGHTFGCPNCSA
                     KMVLHQRARQLRCHHCDHREPIPFKCPDCGNQDLTAVGHGTQRVEETLRAFLPKAAVV
                     RVDRDSTAHKNDWADLYRRIADNEIDILVGTQMLAKGHDFARLNLVIVLNADGSLYSA
                     DFRAPERLFAELMQVSGRAGRADKPGKVLIQTQLPEHPVFAAVKAQDYAVFAENELNE
                     RQMFSMPPFGFQTAVRADAPRVADAMEFLNAAKETLAPLLPESVSQFGAAPMLMVRLA
                     ERERAQIFLESTSRQDLHRAVSLWVQVLQQNRDGKIRWSVDVDPQEA"
     gene            5585..6367
                     /locus_tag="WGQ68_000305"
     CDS             5585..6367
                     /locus_tag="WGQ68_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HEZ0714183.1"
                     /translation="MKTKLIKILTPFTVLPLLACGQTPVSNANAEPAAKAESAGKSVA
                     ASLKARLEKTYSAQDLKVLSVSETPVKGIYEVVVSGRQIIYTDAEGGYMFVGELINID
                     TRKNLTEERAADLNKIDFASLPLDKAIKEVRGNGKLKVAVFSDPDCPFCKRLEHEFEK
                     MTDVTVYSFMMPIAGLHPDAARKAQILWCQPDRAKAWTDWMRKGKFPVGGSICDNPVA
                     ETTSLGEQFGFNGTPTLVFPNGRSQSGYSPMPQLEEIIRKNQ"
     gene            complement(6450..6672)
                     /locus_tag="WGQ68_000306"
                     /pseudo
     CDS             complement(6450..6672)
                     /locus_tag="WGQ68_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250036.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6686..8620)
                     /locus_tag="WGQ68_000307"
     CDS             complement(6686..8620)
                     /locus_tag="WGQ68_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacB family efflux pump subunit"
                     /protein_id="HEZ0714184.1"
                     /translation="MSLIECKNINRYFGSGENRVHILKDISLSIEKGDFVAIIGQSGS
                     GKSTLMNILGCLDTAGSGSYRIDGIETAKMQPDELAALRRERFGFIFQRYNLLSSLTA
                     RDNVALPAVYMGAGGKERSARADKLLQDLGLASKEGNKPGELSGGQQQRVSIARALMN
                     GGEIIFADEPTGALDTASGKNVMEIIHKLHEAGHTVIMVTHDPGIAANANRVIEIRDG
                     EIISDTSKNPEIPASNVGRIREKASWSFYYDQFVEAFRMSVQAVLAHKMRSLLTMLGI
                     IIGIASVVSVVALGNGSQKKILEDISSIGTNTISIFPGRGFGDRRSGRIKTLTIDDAK
                     IIAKQSYVASATPMTSSGGTLTYRNTDLTASLYGVGEQYFDVRGLKLETGRLFDENDV
                     KEDAQVVVIDQNVKDKLFADSDPLGKTILFRKRPLTVIGVMKKDENAFGNSDVLMLWS
                     PYTTVMHQITGESHTNSITVKIKDNANTQVAEKGLTDLLKARHGTEDFFMNNSDSIRQ
                     IVESTTGTMKLLISSIALISLVVGGIGVMNIMLVSVTERTKEIGIRMAIGARRGNILQ
                     QFLIEAVLICVIGGLVGVGLSAAVSLVFNHFVTDFPMDISAASVIGAVACSTGIGIAF
                     GFMPANKAAKLNPIDALAQD"
     gene            complement(8686..9864)
                     /locus_tag="WGQ68_000308"
     CDS             complement(8686..9864)
                     /locus_tag="WGQ68_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MacA family efflux pump subunit"
                     /protein_id="HEZ0714185.1"
                     /translation="MAKMMKWAAVAAVAAAAVWGGWSYLKPEPQAAYITETVRRGDIS
                     RTVSATGEISPSNLVSVGAQASGQIKKLYVKLGQQVKKGDLIAEINSTSQTNTLNTEK
                     SKLETYQAKLVSAQIALGSAEKKYKRQAALWKDDATSKEDLESAQDALAAAKANVAEL
                     KALIRQSKISINTAESELGYTRITATMDGTVVAILVEEGQTVNAAQSTPTIVQLANLD
                     MMLNKMQIAEGDITKVKAGQDISFTILSEPDTPIKAKLDSVDPGLTTMSSGGYNSSTD
                     TASNAVYYYARSFVPNPDGKLATGMTTQNTVEIDGVKNVLIIPSLTVKNRGGKAFVRV
                     LGADGKAAEREIRTGMRDSMNTEVKSGLKEGDKVVISEITAAEQQESGERALGGPPRR
                     "
     gene            10090..10479
                     /locus_tag="WGQ68_000309"
     CDS             10090..10479
                     /locus_tag="WGQ68_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0714186.1"
                     /translation="MKNVQKGFTLLELMIAVAILGILTLITYPSYKTYIRRVRLTEVR
                     TTLLHNAQTMERYYRQKGTFKTYDKNKLKQNEYFDITLSEVSPDHFTLKATPNPTTND
                     GETCVVTLNDGGIIAASGTNQSCPGFD"
     gene            10762..11808
                     /gene="adhP"
                     /locus_tag="WGQ68_000310"
     CDS             10762..11808
                     /gene="adhP"
                     /locus_tag="WGQ68_000310"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEZ0714187.1"
                     /translation="MKMQAVVVNKNVAGDVEIVEREVRPLEYGEALVEVEYCGVCHTD
                     LHVAAGDYGEKPGRVLGHEGIGLVKEVADGVKNLKVGDRVSIAWLFQSCGSCEYCNTG
                     RETLCRSVLNAGYTADGGMATYCIVSADYAVKVPEGLDPAQASSITCAGVTTYKAIKV
                     SSVRPGQWIAIYGAGGLGNLGVQYAKKVFGAHVVAIDINDDKLAFAKETGADLVVNAA
                     KEDAAKVIQEKTGGAHAAVVTAVSAAAFNSAVNCVRAGGRVVAIGLPPESMDLSIPRL
                     VLDGIEVVGSLVGTRKDLEEAFQFGAEGLVVPKVQLRALDEAPAIFQEMREGKITGRM
                     VIDMKKECGCGHHH"
     gene            complement(12159..15644)
                     /gene="smc"
                     /locus_tag="WGQ68_000311"
     CDS             complement(12159..15644)
                     /gene="smc"
                     /locus_tag="WGQ68_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEZ0714188.1"
                     /translation="MRLTHIKLSGFKSFTDPTTIHVPGQLVAVIGPNGCGKSNVIDAV
                     RWVLGEASAKQLRGESMQDVIFNGAATRRPAPRASVELVFDNSDHSLQGAWGQYAEVS
                     IKRQLTRQGESTYFINNQTVRRRDITDLFLGTGVGARGYAVIEQGMISRIIEARPEEL
                     RAYIEEAAGVSKYKERRKETEGRLKDTREHLQRLGDLQNELARQVEKLEKQAETAERY
                     KSLTAQLNRQQDLLDYAQWQQSLAAADKATEQHQSLQAQQDETAAQVQALNDEVHALQ
                     TAEQSQQQAVHELSNKRGVLREQIARLEEQIRHQQNLHQRIERDKQAAQAQMQRIHQE
                     QQQIRVQLEENELYVEEKQTELAEWAMQVAEHEERLPELEETQTALNAAFQTQQDEAN
                     RIRRELALKQQQLAHAEQTVAKHEERKGRLKQENQALNLPDEAETAAAQEAAALLQSR
                     QEHYEEQIIAAEEALHAAREAFQTASNRFQSLKQQHITLQAQQQALSQILSQQQEAAD
                     FWQATGHAAAPQLWQHITAPAEWQHALSVILAERLHARDVPQGFVPPEPLPQGQAAWL
                     SDGLSGGIKKSLPVQALLNQIQAQPPFQTALHHWLDGVLCAPDLSYALAHQSDLGAHQ
                     IWLTPEGHQVDKVSVLLYAKPAQESLIAQKARLDGIASELESLAPELSAAEAAFKQAE
                     AAVRSSEVQHKNLMQQQQQHTRQYSQAQQRAAELLARTNQGQIRREHIERELAQLAEE
                     QTVLQHASDGLSDDIATLQEAAAELEHQQQTTAHSRQEQQGRLKQAQLALLEANRQYG
                     LAEVAVHKLNQQKQNYQQQIARLEQQTLDWQERQQELVLAYETEFQNDEQHIKLDELT
                     EAVQTLDEEYIAVQDKLAQIQAQGREQYARVQTLQTQLPQLQAATQTALLQQQEALIN
                     AKRYHQNLTERAADLDALEALAKESPKVLNSSIGSLSQQIEALGAVNLAALQELEEAR
                     ERDGYYRSQSEDVQAAITLLEEAIAQIDDKTKARFKETFDAVNSKVQTFFPTLFGGGE
                     ATLKMIGDDLLTAGVSIMARPPGKKNSTIHLLSGGEKALTAMSLVFALFSLNPAPFCL
                     LDEVDAPLDDANTSRFCRLVKEMSAQTQFLYISHNRLTMEMAEQLVGVTMQEKGVSRV
                     VAVDIKQALEMAEAV"
     gene            complement(15723..15869)
                     /locus_tag="WGQ68_000312"
     CDS             complement(15723..15869)
                     /locus_tag="WGQ68_000312"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714189.1"
                     /translation="MSWRIITRPAAGQYGKCRAKHRALAEPGFGALKMPSEGFRRHRG
                     CGMR"
     gene            15893..16615
                     /gene="lpxH"
                     /locus_tag="WGQ68_000313"
     CDS             15893..16615
                     /gene="lpxH"
                     /locus_tag="WGQ68_000313"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HEZ0714190.1"
                     /translation="MKPAYFISDLHLSEKQPELTALLLRFLRSSAAGQARAIYILGDL
                     FDFWVGDDEVSELNTSVAREIRKLSDKGVAVFFVRGNRDFLIGQDFCRQAGMTLLPDY
                     SVLDLFGCKTLICHGDTLCTDDKAYQRFRKIVHRKRLQKLFLMLPLKWRTRLAAKIRR
                     VSKMEKQVKPADIMDVNAAFTARQVRAFNAERLIHGHTHREHIHHENGFTRIVLGDWH
                     NDYASILRVDGDGAVFVPLEKC"
     gene            16884..18470
                     /locus_tag="WGQ68_000314"
     CDS             16884..18470
                     /locus_tag="WGQ68_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0714191.1"
                     /translation="MALFLSIFPIVLLIWLMVKKNSMPSYVALPITAVLIYAIKLFYF
                     GDAGMLLNATAASGLVKTLTPITVIFGAIMFNRMMETTGCIDVIRKWLATISPNPVAQ
                     LMIIGWAFAFMIEGASGFGTPAAIAAPILMSLGFNPLKVAIFTLVMNSVPVSFGAVGT
                     PTWFGFAPLNLSAEDILAIGRQTGVMHFFAGFVIPVIGLGFIVPWSEIRKNLGFVAIA
                     VFSCTIPYVALAMVNEEFPSLVAGAIGLMVSVFAANQGWGLSKDHAKDPNAEKVPFAQ
                     VAKALAPLGMLIGMLVVTRIKQLGIKGILTSKEEWFSFQLPFDLSKITVSDSLTITFG
                     NIFGQDVSASYQTLYVPAWIPFVLTVWICILLYKTKFKDAWTIYSVTFNQTKKPLLAL
                     MGALIMVQLMLVGGDNSMVKIIGKEFAAMAGEHWVYFSPYLGAIGAFFSGSNTVSNLT
                     FGPIQQQIALDTGLSVTLILALQSVGGAMGNMVCLNNIIAVCTVLDVKNSEGAIIKKT
                     VIPMAIYGVIAVVAAMIFFL"
     gene            complement(18639..18842)
                     /locus_tag="WGQ68_000315"
     CDS             complement(18639..18842)
                     /locus_tag="WGQ68_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEZ0714192.1"
                     /translation="MDNLNPQEISVLPENLPLYCSGPDNEQWNGHPRVFLPLCEGESG
                     SVACPYCGTRYRLDGKMPHHHYA"
     gene            18958..19455
                     /locus_tag="WGQ68_000316"
     CDS             18958..19455
                     /locus_tag="WGQ68_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-mimic protein DMP19"
                     /protein_id="HEZ0714193.1"
                     /translation="MTALTLPEDIRQQEPSALLYTLVSAYLEHTAQTGDESLSCLSDD
                     QHTLTAFCYLDSQVEEGGFVQLIASGYGEYIFRNPLADSLRRWKIKAVPKVLDKAKAL
                     YEQHGKTIETLADGGADIESLRKQFPDFEEWDGAYYEAAEQDLPLLAEHIQSNWETFA
                     HIGQA"
     gene            19537..20730
                     /locus_tag="WGQ68_000317"
     CDS             19537..20730
                     /locus_tag="WGQ68_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/tyrosine/aromatic aminotransferase"
                     /protein_id="HEZ0714194.1"
                     /translation="MFFKHIEAAPADPILGLGEAFKAETRPEKVNLGIGVYKDASGAT
                     PLVKAVKEAEKRLLESETTKNYLTIDGVADYNAQTQILLFGKDHEIIASRRAKTAQSL
                     GGTGALRIAAEFAKRQLNAQTIWISNPTWPNHNAIAKAVGIQDKPYRYYDAAKHGLDW
                     DGMIEDLSQAQKGDIVLLHGCCHNPTGIDPTPEQWETLAKLSAEKGWLPLFDFAYQGF
                     GNGLEEDAYGLRVFLKHNTELLIASSYSKNFGMYNERVGAFTLVAEDEATAARAHSQV
                     KTIIRTLYSNPASHGANTIALVLENDDLKAQWIAELDEMRGRIKAMRQKFVELLKAKG
                     ASQNFDFIIEQNGMFSFSGLTPEQVDRLKNEFAIYAVRSGRINVAGITDDNIDYLCES
                     IVKVL"
     gene            complement(21134..22069)
                     /gene="hemC"
                     /locus_tag="WGQ68_000318"
     CDS             complement(21134..22069)
                     /gene="hemC"
                     /locus_tag="WGQ68_000318"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEZ0714195.1"
                     /translation="MNPKKLVIASRESLLAMWQAKHIQGRLKALYPDCEVEILGMTTR
                     GDQILDKTLSKVGGKGLFVKELEQALYDGRADLAVHSIKDVPMDLPEGFALATIGERA
                     NPFDAFVSNQYTRLEEMPEGAVVGTSSLRREAQLRARYPHLLIKPLRGNVQTRLSKLD
                     NGEYDAIILAAAGLQRLKLDGRIRMILSESDSLPAAGQGALGIEIAAHREDLYEVLKP
                     LNHGVTNACVTAERALARALGGSCQVPLAAYCTEENGLLTLRGLVGHPDGSVVLRADA
                     QAPAEYADALGRAVAKKLADDGAQELIGAVLNTEN"
     gene            22188..22703
                     /gene="ybeY"
                     /locus_tag="WGQ68_000319"
     CDS             22188..22703
                     /gene="ybeY"
                     /locus_tag="WGQ68_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEZ0714196.1"
                     /translation="MKRAKKYPFLTLQRQRFHLNFENASSAAGIPAERDFYRWAWSAL
                     KNEYRRADISLILLDEEEARAYNRDYRGKDYATNVLSFALNEGETLPGQVSEKLYGDL
                     IICPQVVLKEAAEQGKTPERHFAHLTIHGVLHLMGYDHIEDDEAEIMEAEEIRLMRAA
                     GFPNPYQEDGH"
     gene            22705..23529
                     /locus_tag="WGQ68_000320"
     CDS             22705..23529
                     /locus_tag="WGQ68_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEZ0714197.1"
                     /translation="MDGAQPKTNFFERLIARLAREPDSAEDVLNLLRQAHEQEVFDAD
                     TLLRLEKVLDFSDLEVRDAMITRSRMNVLKENDSIERITAYVIDTAHSRFPVIGEDKD
                     EVLGILHAKDLLKYMFNPEQFHLKSILRPAVFVPEGKSLTALLKEFREQRNHMAIVID
                     EYGGTSGLVTFEDIIEQIVGEIEDEFDEDDSADNIHAVSSERWRIHAATEIEDINTFF
                     GTEYSSEEADTIGGLVIQELGHLPVRGEKVLIGGLQFTVARADNRRLHTLMATRVK"
     gene            complement(23659..25038)
                     /gene="nhaC"
                     /locus_tag="WGQ68_000321"
     CDS             complement(23659..25038)
                     /gene="nhaC"
                     /locus_tag="WGQ68_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEZ0714198.1"
                     /translation="MFAFKSLLDMPRGEALAVVVALIAAMGYTIISLEWLPHMSIIAA
                     IVVLILYGLARGLKYNDMQQGMIGALNQGMGAIYLFFFIGLMVSALMMSGAIPTLMYY
                     GFGLISPTYFYFSAFALCSVIGVSIGSSLTTCATVGVAFMGMAAAFHADMAMTAGAIV
                     SGAFFGDKMSPLSDTTGISASIVGIDLFEHIKNMMYTTIPAWLISAALMLWLLPSVAA
                     QDLNSVESFRSQLEATGLVHGYSLIPFALLVVLALMRVNAVVAMLFTVMVAVAVTYLH
                     STPDLRQLGAWFYGGYKLEGEAFKDIAKLISRGGLESMFFTQTIVILGMSLGGLLFAL
                     GAIPSLLDAVRSFLTNAGRATFSVAMTSVGVNFLIGEQYLSILLSGETFKPVYDKLGL
                     HSRNLSRTLEDAGTVINPLVPWSVCGVFISHALGVPVWEYLPYAFFCYLSLALTLLFG
                     WTGLTLSKK"
     gene            complement(25329..26552)
                     /locus_tag="WGQ68_000322"
     CDS             complement(25329..26552)
                     /locus_tag="WGQ68_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar MFS transporter"
                     /protein_id="HEZ0714199.1"
                     /translation="MSAQSQNNHTSPLVVLTTLFFMMGFITCMNDILIPHLKEIFDLS
                     YVQAMLIQFCFFTAYAVMSIPMGAFVGKVGYKNGVIGGFLLTAVGCLLFYPAAGSHSY
                     AVFLGALFILASGVTLLQVAGNPYVTLLAKPGKESATLTLVQAFNALGTTIAPQIGAF
                     LILADATQTVSKAEQISSVQIPYLGLAGLLIILAVFVKMIRLPDARKIAAEESAHNHD
                     GKTSVWQYKHLVFGTAGIFCYVGAEVSIGSLMVNVLGYLKGLDHASAAHYLSFYWGGA
                     MVGRFLGSAVMAKFAPNRYLAFNASAAVVLLAVAMATGSGNADVAMWSLLAIGFFNSI
                     MFPTIFSLATKGLGKFTNAASGVLCTAIVGGAVVPVVQGWVADTYTLMSSFVVSVICY
                     LYIVFFAVYGYRADK"
     gene            27142..27555
                     /locus_tag="WGQ68_000323"
     CDS             27142..27555
                     /locus_tag="WGQ68_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0714200.1"
                     /translation="MIPSYTYAALAFSAFTSATLLPGTSEAAFALFVRRFPEHAYGAL
                     LCAGLANGLGSMVSYWMGRLLPSRKMPSEKTLNLMRRFGIWLLPFAWLPVVGDALPLA
                     AGWLRLNPWTSGLMLVIGKTARYAFILWGMQYYAA"
     gene            27601..28230
                     /gene="nth"
                     /locus_tag="WGQ68_000324"
     CDS             27601..28230
                     /gene="nth"
                     /locus_tag="WGQ68_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEZ0714201.1"
                     /translation="MNRHIRQEIFERFRAANPHPTTELNFNSPFELLIAVLLSAQATD
                     VGVNKATAKLFPVADTPQAMLDLGLDGVMEYTKTIGLYKTKSKHIMQTCRILLEKYNG
                     EVPEDREALESLPGVGRKTANVVLNTAFGHPVMAVDTHIFRVSNRTKIAPGKDVREVE
                     DKLMRFIPKEFLMDAHHWLILHGRYTCKALKPQCQTCIINDLCEYPAKA"
     gene            28368..29867
                     /locus_tag="WGQ68_000325"
     CDS             28368..29867
                     /locus_tag="WGQ68_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HEZ0714202.1"
                     /translation="MFKKYQYLALAALCAASLAGCDKAGSFFGADKKEASFVERIEHT
                     KDDGSVSMLLPDFVQLVQSEGPAVVNIQAAPAPRTQNGSGNAETDSDPLADSDPFYEF
                     FKRLVPNMPEIPQEEADDGGLNFGSGFIISKDGYILTNTHVVTGMGSIKVLLNDKREY
                     TAKLIGSDVQSDVALLKIDATEELPVVKIGNPKDLKPGEWVAAIGAPFGFDNSVTAGI
                     VSAKGRSLPNESYTPFIQTDVAINPGNSGGPLFNLKGQVVGINSQIYSRSGGFMGISF
                     AIPIDVAMNVAEQLKNTGKVQRGQLGVIIQEVSYGLAQSFGLDKAGGALIAKILPGSP
                     AERAGLQAGDIVLSLDGGEIRSSGDLPVMVGAITPGKEVSLGVWRKGEEITIKVKLGN
                     AAEHIGASSKTDEAPYTEQQSGTFSVESAGITLQTHTDSSGGHLVVVRVSDAAERAGL
                     RRGDEILAVGQVPVNDEAGFRKAMDKAGKNVPLLVMRRGNTLFIALNLQ"
     gene            30069..31589
                     /locus_tag="WGQ68_000326"
     CDS             30069..31589
                     /locus_tag="WGQ68_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HEZ0714203.1"
                     /translation="MQLIDYSHSFFSVVPPFLALALAVITRRVLLSLGIGILVGVAFL
                     VGGNPVDGLTHLKDMVVGLAWSDGDWSLGKPKILVFLILLGIFTSLLTYSGSNQAFAD
                     WAKRHIKNRRGAKMLTACLVFVTFIDDYFHSLAVGAIARPVTDKFKVSRAKLAYILDS
                     TAAPMCVLMPVSSWGASIIATLAGLLVTYKITEYTPMGTFVAMSLMNYYALFALIMVF
                     VVAWFSFDIGSMARFEQAALNEAHDETTVSDATKGRVYALIIPVLALIASTVSAMIYT
                     GAQASETFSILGAFENTDVNTSLVFGGTCGVLAVVLCTLGTIKTADYPKAVWQGAKSM
                     FGAIAILILAWLISTVVGEMHTGDYLSTLVAGNIHPGFLPVILFLLASVMAFATGTSW
                     GTFGIMLPIAAAMAVKVEPALIIPCMSAVMAGAVCGDHCSPISDTTILSSTGARCNHI
                     DHVTSQLPYALTVAAAAASGYLALGLTKSALLGFGTTGIVLAVLIFLLKDKKRANA"
     gene            31646..32731
                     /gene="nagZ"
                     /locus_tag="WGQ68_000327"
     CDS             31646..32731
                     /gene="nagZ"
                     /locus_tag="WGQ68_000327"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEZ0714204.1"
                     /translation="MTVPCIPRGLVMADIAAFRLTEEEKQRLLDPAVGGVILFRRNFQ
                     NIAQLKELTAEIKALRTPELIIAVDHEGGRVQRFIEGFTRLPAMSTLGEIWDKDGASA
                     AETAAGQVGRVLATELSACGIDLSFTPVLDLDWGNCPVIGNRSFHRNPEAVARLALAL
                     QKGLEKGGMKSCGKHFPGHGFVEGDSHLVLPEDGRSLSELEAADLAPFRIMSREGMAA
                     VMPAHVVYPQVDTKPAGFSEIWLKQILRRDIGFKGVIFSDDLTMEGACGAGGIKERAR
                     ISFEAGCDIVLVCNRPDLVDELREDFRIPDNPALAQRWQYMANTLGSAAAQAVMQTTD
                     FQAAQAFVAGLASPQDTAGGVKVGEAF"
     gene            32812..32888
                     /locus_tag="WGQ68_000328"
     tRNA            32812..32888
                     /locus_tag="WGQ68_000328"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32846..32848,aa:Pro,seq:cgg)
     gene            complement(33225..33863)
                     /locus_tag="WGQ68_000329"
     CDS             complement(33225..33863)
                     /locus_tag="WGQ68_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HEZ0714205.1"
                     /translation="MKKINVAPENPQYRIVEIFESLQGEGWNTGMPAVFVRLGKCNLA
                     CGWCDTDYLTFGMMSLSDILGCLKTYAARNIIITGGEPTIQPHLDMLLDTLKAEGYFL
                     CLETNGLNPAPPQIDYVATSPKACYAAKYENSCIETADEVRIVADGDVLAFCENMERK
                     IRAHHYYLSPCEQDGAMNIHDTIRQIGILNSRPDAPVHWQLSVQTHKWAGIE"
     gene            complement(33860..34231)
                     /locus_tag="WGQ68_000330"
     CDS             complement(33860..34231)
                     /locus_tag="WGQ68_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEZ0714206.1"
                     /translation="MKLLFILLVLFVAVEHFYIAWLEMTQIPSEKAAEIFKLPYEFME
                     QKRVQTLFGNQGLYNGFLGIGLVWSRFAAPDNAVYGAMVLFLGFVLIAAAWGAFSSGN
                     KGILVKQGLPAFLAAAAVLAV"
     gene            complement(34500..34922)
                     /gene="queD"
                     /locus_tag="WGQ68_000331"
     CDS             complement(34500..34922)
                     /gene="queD"
                     /locus_tag="WGQ68_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEZ0714207.1"
                     /translation="MKITKIFTFDSSHMLDGHDGKCQNLHGHTYKLEITVSDDPVRGG
                     PKDGMVMDFTDLKAIVKQHITDPFDHAFIYHGGNGRESQIAALLEGWNMKTLRLPCRT
                     TAENMAVEMYDRLKNAGLNVCRVKLWETPTSCAEYEGG"
     gene            complement(34929..35447)
                     /locus_tag="WGQ68_000332"
     CDS             complement(34929..35447)
                     /locus_tag="WGQ68_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1543 domain-containing protein"
                     /protein_id="HEZ0714208.1"
                     /translation="MPKLHMFYLGGNAGRSNIEVHDIQFAVCDDYREAVPALKAAWFG
                     DADKIHIDGWQIVEWADGYDIAVSETPKTKMPSEHAPRLYFANVGGYRAGQLAEAHAF
                     GLFAAATPAEAKQKALQTLLTDSYVQQHKDNLKDVDNLLALDRIGNFHIRLTPNPHGK
                     PAEIGFQGYLPI"
     gene            complement(35475..36137)
                     /gene="queC"
                     /locus_tag="WGQ68_000333"
     CDS             complement(35475..36137)
                     /gene="queC"
                     /locus_tag="WGQ68_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEZ0714209.1"
                     /translation="MSNQQALVIFSGGQDSTTCLIQAIQTYGRENVQAITFQYGQRHA
                     VELERARWIAQDLGIKQTVLDLSLMRQITHNALMDDTAAIETAENGVPNTFVDGRNAL
                     FLLYAAIYAKGQGIRHIIAGVCETDFSGYPDCRDVFVKSMNVTLNLAMDYDFQIHTPL
                     MYLTKAQTWALADEMGALDYIREQTHTCYNGIVGGGCRECPSCILRERGLAEYLESKK
                     AV"
     gene            36242..36667
                     /locus_tag="WGQ68_000334"
     CDS             36242..36667
                     /locus_tag="WGQ68_000334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714210.1"
                     /translation="MPSEHAAYADLTASSAKRLLCAKAILFSSAVRTGAAVGNIFFDG
                     FADGGDFGDGAQYGFATFNAVDAASDSAGGIAGAAVLGIVEDEDFEGDLGSWADSVKM
                     VRSSNGITVVPMPSETAAVQAFVPDGKIGYNIGHQCPTY"
     gene            36682..37908
                     /locus_tag="WGQ68_000335"
     CDS             36682..37908
                     /locus_tag="WGQ68_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY family inner membrane protein"
                     /protein_id="HEZ0714211.1"
                     /translation="MTFLQRLQGLADNKICAFAWFVVRRFDEERVPQAAASMTFTTLL
                     ALVPVLTVMVAVASIFPVFDRWSDSFVSFVNQTIVPQGADMVFDYINAFREQANRLTA
                     IGSVMLVVTSLMLIRTIDNTFNRIWRVNSQRPWMMQFLVYWALLTFGPLSLGVGISFM
                     VGSVQDAALASGAPQWSGALRTAATLTFMTLLLWGLYRFVPNRFVPARQAFVGALATA
                     FCLETARSLFTWYMGNFDGYRSIYGAFAAVPFFLLWLNLLWTLVLGGAVLTSSLSYWQ
                     GEAFRRGFDSRGRFDDVLKILLLLDAAQKEGKALPVQEFRRHINMGYDELGELLEKLA
                     RHGYIYSGRQGWVLKTRADSIELNELFKLFVYRPLPVERDHVNQAVDAVMMPCLQTLN
                     MTLAEFDAQAKKQQQS"
     gene            complement(38290..38424)
                     /locus_tag="WGQ68_000336"
                     /pseudo
     CDS             complement(38290..38424)
                     /locus_tag="WGQ68_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(38423..38881)
                     /locus_tag="WGQ68_000337"
     CDS             complement(38423..38881)
                     /locus_tag="WGQ68_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714212.1"
                     /translation="MLTKLKILLFLFLFVFVLAINLLFFFFSSDIESFGNYQFEYVYD
                     KGWPANYILVMKDGNEGNFDKIISGLVLEYYKEDDNIYFSYIDGQGFASDSCYYKPEI
                     LYGKIILNKNHIININSMEKNNFLSEDKIMKGTRNWLADPKNKCNIQTLD"
     gene            complement(39445..>39626)
                     /locus_tag="WGQ68_000338"
     CDS             complement(39445..>39626)
                     /locus_tag="WGQ68_000338"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714213.1"
                     /translation="EGFTIGGGLVLGGCAGAHLARKESLILTGKTGAGASAIANASIG
                     YQWTVNLSKPKEGAK"
BASE COUNT         9214 a        11058 c        10184 g         9170 t
ORIGIN      
        1 cgggcagcct atttcggact gattaaagtg agtgcgcaaa gccatctgaa ggcgatgtgt
       61 ttgaacctgt tgaaagccgc caacaggcta agtgcgcctg ctgccgccta aaaggcggct
      121 cggatgcctg attatcgggt atccggggag gattcagggg gtatttgggt aaaattagga
      181 ggtatttggg gcggaaatag acgaaaacct gtgtttgggt ttcggctgtc gggagggaaa
      241 ggaattttgc aaaggtctcc gaccgcttcc cgctattgaa gttggatcaa gtgattgact
      301 ggcaaccgat cgaacaatac ctgaatcgtc aaagaacccg ttaccttaga gaccatcgtg
      361 gccgtcccgc ctatcccctg ttgtccatgt tcaaagccgt cctgcccgga caatggcaca
      421 gcctctccga tcccgaactc gaacacagcc tcattacccg catcgatttc aacctgtttt
      481 gccgttttga cgaactgagc atccccgatt acagcacctt atgccgctac cgcaattggc
      541 tggcgcaaga cgacaccctg tccgaattgc tggaactgat taaccgccaa ctgaccgaaa
      601 aaggtttaaa aatagagaaa gcatccgccg ccgtcgttga tgccaccatt attcagaccg
      661 ccggcagcac acagcgtcag gtcatagaag tcgatgaaga aggacaaatc agcggccaaa
      721 ccacaccgag taaagacaaa gatgcccgtt ggataaagaa aaacggcctc tacaaactcg
      781 gttacaaaca acatacccgt accgatgcgg aaggctatat cgagaaactg cacatcaccc
      841 ccgccaatgc ccatgagtgc aaacacctgc cgccgttgtt ggaaggactg cccaaagata
      901 cgaccgtcta tgccgacaaa ggctacgaca gtgcggaaaa ccggcaacat ctggaagaac
      961 atcagttgca ggacggcatt atgcgcaaag cctgccgcaa ccgtccgctg acggaagcgc
     1021 aaaccaaacg caaccgatat ttatcgaaga cccgtggcga tgtgtttgaa cctgttgaaa
     1081 gccgccaaca ggctaagtgc gcccgctgcc gcccaaaagg cgaccggatg cctgattatc
     1141 gggtatccag ggaggattaa gggggtattt gggtaaaatc aggaggtatt tggggcgaaa
     1201 acagccgaaa acctgtgttt gggtttcggc tgtcgggagg gaaaggaatt ttgcaaaggt
     1261 ctcaggtcgc cctcgtcgtg acggaaagta ctggcggtct cgaaatcccc gccgccaaag
     1321 ccatccgccg agcaggcata gccgtgatta tcgccaaccc gcgtcagacg catcagtttg
     1381 cccaatcgca gccgctgacc aaaaccgacg ccaaagatgc caaaatgctc gccttcttcg
     1441 cacagatgat gacgcagaaa gaagattggc aaaccatgcc ctaccaaccg cccacagaag
     1501 cggaagaagt gttggaagcc ttggttaacc gccgcaacca atcggcggat atgcggactg
     1561 ccgagaaaaa ccgtctgcat caggttcacg aaacgcaagt cggaagcgtc aaacaactga
     1621 ttgcccattt tgaccggctg attgacgaat tggacaaaca aatcgacgac cacacccaca
     1681 cgcattttga cggcaaagcc caagtggcgg agcaaatcaa aggcatcggt tcgataacga
     1741 cggctacgct gatggcgatg ctgcctgagt tggggcggct gtcgcacaaa cggatagcga
     1801 gtttggccgg cattgccccg cacccgaggg agagcgagga aaccaaattc aaaagccgct
     1861 gcttcggcgg aaggtctgcg gtacgtaagg cgctgtatat ggctaccgtg gtagcgacac
     1921 gttttaaacc gcttattcgg gatttctacc aacgcctgcc gtccgagggt aagccgtata
     1981 aggttgccgt tacggcatat atgcgcaaac tgctgacgat atcgaatgcc cggatgcgtg
     2041 attattttgc cgaaaacgat accgccgaaa acggtatccg gacggcttga tttgagtttt
     2101 ggtatttttg cccgacgggg tgaaaaatac agttgctatg cgcccgtttt tcgtcggcgc
     2161 ggcagtgctt gccatactcg gcgcgttggt gtttttcatc agccccggcg ccatcgtcct
     2221 gcaccgccaa attttcttgg aacttatgct gccggcggca tacggcggtt ttttgactgc
     2281 ggctttgttg gattggacag gtttttcggg caacctgaaa cctgccgcta ctttgatggc
     2341 ggtgttgttg cttgttgcgg ctgttttatt gccgttttta ccgcaacttg ccgcattctt
     2401 tgtagcggct tattggctgg tgttgctgct gttctgcgcc cggctgattt ggctagatcg
     2461 aaacaccgac aactttgccc tcttaatgct gctcgccgcg ttcacggttt ttcagacggc
     2521 atacgcggca actggcgatt taaacctgtt gcgcacgcaa gtgcatctga atatggcggc
     2581 ggtgatgttt gtttccatac gcgtgagtgt tttgatgggc gcggaagcgt tgaaagaatg
     2641 ccgtctgaaa gaccccgtat tcatccccaa cgtcgtctat aaaaacattg ccatcacctt
     2701 cctgctgctg cacgccgccg ccgaactttg gctgcccacg cagaccgctg gttttaccgc
     2761 gctcgccgtc ggcttcatcc tgctcgccaa gctgcgtgaa cttcaccatc acgaactcct
     2821 gcgcaaacac tacgtccgca cttattacct gctccagctc tttgccgcgg caggctatct
     2881 gtggacaggc gcggcgaaat tgcaaaacct gcccgcctcc gcgcccctgc acctgattac
     2941 cctcggcggc atgatgggcg gcgtgatgat ggtgtggctg actgccggac tgtggcacag
     3001 cggctttacc aagctcgact acccgaaact ctgccgcatc gccgtcccca tcctcttcgc
     3061 cgccgccgtc tcgcgcgctg ttttaatgaa cgtaaacccg atattcttca tcacactccc
     3121 tgcgattctg accgccgccg tgttcgtgct ttatcttttc acgtttgttc cgatttttcg
     3181 ggcgaacgcg tttacgggcg atccggaata aaatgccgtc tgaaacggtt ttcagacggc
     3241 atcgtattgc cgataatcaa gcctcctgcg gatcgacatc caccgaccat ctgattttgc
     3301 cgtcgcggtt ttgctgcaac acctgcaccc acaaactcac ggcacggtgc aaatcttgtc
     3361 gggatgtcga ttcgaggaag atttgcgcgc gttcgcgttc agcaaggcgc accatcagca
     3421 taggggcggc gccaaactgg gaaacgcttt cgggcaacag cggggcgagg gtttctttgg
     3481 cggcgttgag aaattccatc gcatcggcaa cgcgcggcgc gtcggcgcgg acggcggtct
     3541 ggaaaccgaa gggcggcata gagaacattt gccgctcgtt caattcattt tcggcaaaca
     3601 cggcataatc ctgcgctttg acggcggcga agacgggatg ttcgggcagt tgggtctgta
     3661 tcagcacctt gccgggttta tcggcgcgcc ccgccctgcc ggacacttgc atcagctcgg
     3721 caaacagcct ttccggcgcg cgaaagtccg cgctgtacag gctgccgtcg gcgttcaaca
     3781 cgataacgag gttgagccgc gcaaaatcat ggcctttggc gagcatctgc gtgccgacca
     3841 gaatatcgat ttcattgtcg gcgatgcggc ggtacaaatc cgcccagtcg tttttgtgcg
     3901 ccgtgctgtc cctgtcgacg cggacgacgg cggccttggg caggaaggcg cgcagggttt
     3961 cttcaacgcg ctgtgtgccg tgtccgacgg cggtcaggtc ttggttgccg cagtcggggc
     4021 atttgaatgg gatgggttcg cggtggtcgc agtggtggca gcgcagttgg cgggcgcgtt
     4081 ggtgcagcac cattttggcg gagcagttcg ggcagccgaa ggtatggccg cagtcgccgc
     4141 aaaacagcgc gggcgcgaag ccgcgccgat taaggtacac cagcgacata ccgcccgctt
     4201 caaaattctg tttcaaaagc tgcaaggctt gcggcgagaa accgttgtcg agtttcagac
     4261 ggcctacgtt gagaatgtcc acttgcggca gttgcgcggc ggtatgggcg cgttcggtca
     4321 gttgcagcag gcggtacgcg ccactttgcg ccttgtgcca gctctccaag ctgggcgtgg
     4381 cactgcccaa cacgacgggg cagccgctct gcttcgcccg ccacaccgcc aaatcgcggg
     4441 catgatagcg caattcgttg tcttgtttga acgagccgtc gtgttcctca tcgaccacaa
     4501 tcagcccgac atcatccatc ggcgtgaaca ctgccagccg cgtgccgatc accagcttcg
     4561 cctgccctaa catcgcgcgc agataatcct gcgtgcgctt gcctgccgcc atctgactgt
     4621 gcaacacggc ggtcggcacg tcggcaaaac ggttttccac ccgcttcaaa agctgcggcg
     4681 tgaggttgat ttcgggcaac agaaacaaca cctgccgccc ctgcgccaac actttcgcca
     4741 tcgcatcgaa atacacctcg gtcttgccgc tgccggtgat gccatacagc agaaacggtt
     4801 ggaagctgcc aaaggccgtc tgaatggcat cggaagcttt ctgctggttg gcattcaaca
     4861 caaattcaga gtgcgaagcc tgcccgtggt acggccttaa aacgggtttc gccgcttccg
     4921 tcgtttcaat ccaaccctgc accgcccaat cttcaatcaa tttcgccgcc tgcgcgttta
     4981 cctgcttcaa cgctgccatc gtcatttcgc ccgacagcag cgcgtcccac aaagccgctt
     5041 ttttgttgaa ccgcgccggc ggcggcgttt gcgccctgcc cgcttcgttc aacgcgtaaa
     5101 acaagggcgg ctgcggcatt tccaccgcgc gcgtttcctt caaaccttgc ggcaatgcgg
     5161 caaatactgc ctgcccggtc ggatagtggt aataacgcga cgtaaacgcc aacaaatcac
     5221 gccagctttc gggcagcagc ggttcgtccg aaaaggtcgt ctgaacgccc aaaatccgcg
     5281 ccgtatccat atccggcgca atatccgttt cccacaccat cccgaccacg gttttattgc
     5341 ggaaaggcac aagcacccgc gttcccggag gaagcggatc ggaatgggaa taagtcaaaa
     5401 ggccgtctga aagcggcacg tttacggcga tgcggtggta gatcatacag gcaggcaaat
     5461 catttttttg cgtattttaa caggcggaag ctattttcag acgataaatt gagcctaggc
     5521 gcaatgcagg cgcaacagag atttgataca attccgcttt tccaacacgc gcaatcatct
     5581 ttatatgaaa accaagttaa tcaaaatctt gacccccttt accgtcctcc cactgctggc
     5641 ttgcgggcaa acgcccgttt ccaatgccaa cgccgaaccc gccgccaaag ccgagtccgc
     5701 cggcaaatcc gttgccgcct ctttgaaagc gcgtttggaa aaaacctatt ccgcccaaga
     5761 tttgaaagtg ttgagcgtca gcgaaacacc ggtcaaaggc atttacgaag tcgtcgtcag
     5821 cggcaggcag attatctaca ccgatgccga aggcggctat atgttcgtcg gcgaactcat
     5881 caacatcgac acgcgcaaaa acctgaccga agaacgcgcc gccgatttga acaaaatcga
     5941 cttcgcctcc ctgcctttgg acaaagccat caaagaagtg cgcggcaacg gcaagctgaa
     6001 agtcgccgtc ttctccgacc ccgattgtcc gttctgcaaa cgcttggaac acgagtttga
     6061 aaaaatgacc gacgtgacgg tttacagctt tatgatgccc attgccggcc tgcaccccga
     6121 tgccgcgcgc aaggcgcaaa tcttatggtg tcagcccgac cgcgccaaag cgtggacgga
     6181 ttggatgcgt aaaggcaaat tcccggtcgg cggcagcatc tgcgacaatc ccgtcgcgga
     6241 aaccacttcc ttgggcgaac aattcggctt caacggcacg ccgaccctcg tcttccccaa
     6301 cgggcgcagc caaagcggct acagcccgat gccccaactg gaggaaatca tccgcaaaaa
     6361 tcaataatcc atccgcaaaa caaaaggctt ggaaaattcc aagccttttt tgcgggaaat
     6421 gccgtctgaa aaccaatccg cactgaaagt tatagtggat taaatttata gtggattaaa
     6481 tttaaaccag tacagcaagc caacaaacac atcctaaccc attgaatgat tgaaaataat
     6541 aaactgaaca atacgaaaca caccgccgtc attcccacgc aagtgggaat ctagaacgta
     6601 gaatctaaga aaccgttttt gtccaataag tttctgctcc aaaatgaccg gtcctgcagc
     6661 ttcagacggc atctttgttc caacctcaat cctgcgccaa tgcgtctatc ggattgagtt
     6721 tggctgcttt attggcaggc ataaagccga acgcgatgcc gattccggtc gaacaggcga
     6781 ccgccccgat aacggatgcc gccgaaatgt ccatcgggaa gtcggttaca aaatgattga
     6841 acacgaggct gacggcggcg gacaaaccca cgccgaccaa accgccgatg acgcagatta
     6901 acaccgcctc aatcaaaaac tgctgcaaga tattgccgcg ccgcgcgccg attgccatcc
     6961 gtatgccgat ttctttggtg cgctcggtaa cggacaccag catgatgttc atcacgccga
     7021 tgccgccgac taccaatgaa atcagggcga tggaggaaat cagcagcttc atcgtaccgg
     7081 tggtgctttc gactatctgc ctgatgctgt cgctgttgtt catgaagaaa tcttccgtgc
     7141 cgtgccgcgc tttgagcaga tcggtcagcc ctttttcggc aacctgggta ttggcattgt
     7201 ctttgatttt gacggtgatg gagttggtgt ggctctcgcc tgtgatttgg tgcatcaccg
     7261 tcgtataggg cgaccaaagc atcagcacgt cggaattgcc gaaagcgttt tcgtcttttt
     7321 tcatcacgcc gatgacggtc aaggggcgtt tcctgaacaa aatggtttta cccaacggat
     7381 ccgagtccgc aaagagtttg tctttgacat tttggtcgat gacgacgacc tgcgcgtctt
     7441 ctttcacatc gttttcgtca aacagccgcc ccgtttccag cttcagtccg cgcacgtcga
     7501 aatattgttc gcccacgccg tacaaagaag cggtcaggtc ggtattgcgg taagtcagcg
     7561 tgccgccgct cgaagtcatg ggcgtggcgg aagcaacgta gctttgtttg gcgatgattt
     7621 ttgcgtcgtc tatggtcagg gttttaatcc tgccgctgcg cctgtcgccg aagccgcgcc
     7681 ccgggaagat gctgatggtg ttcgtcccta tcgaactgat gtcttcaagg atttttttct
     7741 gcgaaccgtt gcccaatgcg acgacggaaa ccaccgacgc gatgccgatg atgatgccga
     7801 gcatcgtcag aagcgaacgc attttgtgcg ccaatactgc ttgcaccgac attctgaagg
     7861 cttcgacaaa ctggtcgtaa taaaacgacc acgaggcttt ttcccgaatc ctcccgacat
     7921 tgcttgcggg gatttctgga tttttcgagg tgtcggaaat gatttcgccg tcccgaattt
     7981 cgatgacgcg gttggcattg gcggcgatgc cggggtcgtg cgtgaccata atgacggtat
     8041 gcccggcttc gtgcagctta tggatgattt ccatcacgtt tttgccgctg gcggtatcga
     8101 gcgcgccggt cggctcgtcg gcgaagatga tttctccgcc gttcatcagg gcgcgggcga
     8161 tggagacgcg ctgctgctgt ccgcccgaga gttcgccggg cttgttgccc tctttgcttg
     8221 ccaaacccaa atcctgcaag agtttgtccg cccgcgcgga acgctctttg ccgcccgcgc
     8281 ccatatagac ggctggaagc gcgacgttat cccttgcggt cagcgagctt aagaggttgt
     8341 agcgttggaa gatgaaaccg aagcgttcgc ggcgcaatgc cgccagctcg tcaggctgca
     8401 ttttggcagt ttcgatgccg tcgattcggt acgaaccgga accggcggta tccaaacagc
     8461 cgagtatgtt catcagcgtg gacttgcccg aaccggactg cccgatgatg gcgacaaaat
     8521 cgcccttctc tatcgacagg ctgatgtctt tcaaaatatg gacgcggttc tcgccgctgc
     8581 cgaaatagcg gttgatgttt ttacattcga tcaagctcat aatattccgt aaatcaataa
     8641 caaatgccgt ctgaaaccgt ttccgtgttc agacggcata ttcgtttatc ggcgcggcgg
     8701 gccgcctagg gcgcgttcgc cgctttcctg ctgctcggcg gcggttattt cggagatgac
     8761 cactttgtcc ccctctttca acccgctttt tacttcggta ttcatactgt ctctcatacc
     8821 ggtccggatt tcgcgttccg ccgccttgcc gtccgcaccc aacacgcgca caaacgcctt
     8881 gccgccgcga tttttcacgg tcagcgacgg aataatcagc acatttttca cgccgtcgat
     8941 ttcaaccgta ttctgcgtcg tcatccccgt ggcgagtttg ccgtccggat tcggcacaaa
     9001 cgaacgggca taatagtaga ccgcattgga agccgtatcc gtactgctgt tgtagccgcc
     9061 cgacgacatc gtggtcagcc cggggtcgac gctgtcgagc ttcgccttaa tcggcgtatc
     9121 cggttcggac aaaatcgtaa acgaaatatc ctgccccgcc ttcaccttgg taatatcgcc
     9181 ctcggcaatc tgcattttgt tcaacatcat atccagattc gccaattgga caatcgtcgg
     9241 cgtagactgc gccgcgttca cagtctgccc ctcttccacg agaatcgcca ccaccgtgcc
     9301 gtccatcgtt gcggtaatgc gcgtgtagcc caattccgac tcggcggtat tgatggaaat
     9361 tttgctctgt ctgattagag ccttcagctc ggcaacattg gctttggcgg cggcaagcgc
     9421 atcctgcgcg ctttccaaat cttctttaga ggtcgcatca tccttccaca acgccgcctg
     9481 acgcttatat ttcttctccg cgctgcccaa tgcaatctgt gccgacacca gcttcgcctg
     9541 atacgtttcc aacttggatt tttccgtatt gagcgtattg gtctgcgagg tcgaattgat
     9601 ttccgcaatc aaatcgccct ttttaacctg ttgcccgagt ttgacataaa gtttcttaat
     9661 ctgccccgat gcctgcgcgc cgaccgatac caggttggac ggcgaaatct cccctgttgc
     9721 agaaaccgtc cggctgatgt cgccgcgcct gaccgtttcc gtaatataag cagcctgcgg
     9781 ctcgggcttc agataagacc atccgcccca aaccgctgcc gccgcgaccg ccgcaacagc
     9841 cgcccatttc atcatttttg ccatgttttc aattctctgt atgcgtcaat cccataaatc
     9901 ggaagcggat tctaccgcaa gtccctgccc catccaaggc ggcaaactgc cgacaataaa
     9961 aataaccatt tgttttaaat ggcttataca atctgctttc ccgaaaattt caatccgttt
    10021 acatattccg ccatttccga tacggcgtta taatgtcgcc gaccgacaaa caaatggaaa
    10081 cacattgcta tgaaaaacgt tcaaaaaggc tttacgctgc tcgagctgat gattgccgtc
    10141 gccatcctcg gcatcctgac gctcatcacc tacccttcct ataaaaccta catccggcgc
    10201 gtccgcctga cggaagtcag gacgaccctg ctgcacaacg cgcagaccat ggaacgttac
    10261 taccgccaaa aagggacgtt taaaacctac gacaaaaaca aactgaaaca aaacgaatat
    10321 ttcgatatca ctttaagcga agtcagcccc gaccatttta cccttaaggc aacgcccaat
    10381 cccaccacca acgacggcga aacctgcgtc gttacgctca acgacggcgg tatcattgcc
    10441 gcttccggta caaaccaatc ctgccccggc ttcgactaat cctccgccga agcccgcaaa
    10501 cccgtaaaga tgccgtccgg acacagcttc agacggtatc tttacgggtt tgccgtccgg
    10561 tttttcaaac ttgaaacctg ccgcccctca tccggcaacc gccccgatgc cgcgcctttc
    10621 caagctgtta taatataaca tataatctat atttttcaac aacataccac ttgacctgtg
    10681 agatgcggaa gcgtatgctt acccttatat cggacaacat tccgtttaca ggtttttttg
    10741 ccgtaactaa ggagcgcaat catgaaaatg caggcagttg ttgtgaataa aaatgtagcg
    10801 ggcgatgtgg aaatagtcga acgcgaggtt cgcccgttgg aatacggcga ggcgttggtc
    10861 gaagtcgaat attgcggcgt gtgccacacc gacctgcacg ttgcggcagg cgactacggc
    10921 gaaaaaccgg gccgcgtgct gggacacgag ggcatcggtt tggttaaaga agttgccgac
    10981 ggtgtgaaaa atctgaaagt cggcgaccgc gtcagcatcg cctggctgtt ccaaagctgc
    11041 ggctcttgcg aatactgcaa taccggccgc gaaaccctgt gccgctctgt attgaacgcg
    11101 ggctacaccg ccgacggcgg tatggcgacc tactgcatcg tgagtgccga ttacgcggtc
    11161 aaagtccctg aaggtttgga tcctgcgcaa gcttcgagca ttacttgtgc cggcgtgacc
    11221 acttataaag ccattaaagt ttccagtgtt cgtccgggac agtggattgc catctacggc
    11281 gcgggcggtt tgggcaactt gggggttcaa tacgcgaaaa aagtattcgg cgcgcacgtt
    11341 gtcgccatcg acatcaacga cgacaagctg gcatttgcca aagaaaccgg cgcggatttg
    11401 gttgtcaacg ccgccaaaga agacgctgcc aaagtgattc aggaaaaaac cggcggcgca
    11461 cacgctgcgg tcgtaaccgc cgtatctgct gccgcattca actctgccgt gaattgcgtc
    11521 cgcgcgggcg gacgtgtggt tgccatcggg ctgccgccgg aatcgatgga tttgtccatc
    11581 ccgcgtttgg ttttggacgg catcgaagtg gtcggctctt tagtcggcac gcgcaaagat
    11641 ttggaagaag ccttccaatt cggcgcggaa ggtttggttg tgccgaaagt ccaactgcgt
    11701 gctttggatg aagcacccgc cattttccaa gaaatgcgcg aaggcaaaat caccggccgt
    11761 atggtaatcg atatgaaaaa agaatgcggc tgcggccatc accactgatt tgacgtggca
    11821 gtacacatcg aaatgccgtc tgaacgctgt tcagacggca ttttttatgg atttggattt
    11881 gattttaatc tgttctgttt gaaataccgt ctgaaaaccc gtatcgcaac acgtcattat
    11941 agtggattaa caaaaatcag gacaaggcgg cgagccgcag acagtacaaa tagtacggaa
    12001 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgagcca
    12061 acgctatacc gatttaaatt taattcacta taccgtctga aaacctgtat cgcaacacgt
    12121 cattaaaaac ggcaagattc agccgttctg caacccgttc aaacggcttc cgccatttcc
    12181 aacgcctgtt tgatgtccac ggcgacgacg cgcgatacgc ctttttcctg catggttacg
    12241 ccgacgagct gctccgccat ttccatcgtc aggcggttgt gggagatgta gaggaactgg
    12301 gtttgcgccg acatttcttt gaccagcctg cagaaacgcg aggtgttggc gtcgtccagc
    12361 ggggcatcaa cttcgtccag aaggcagaag ggtgcggggt tgaggctgaa cagggcgaac
    12421 acgaggctca tggcggtgag ggctttttct ccgccggaaa ggaggtggat ggtgctgttt
    12481 ttcttgccgg gcgggcgcgc catgatggac acgccggcgg tcaggaggtc gtcgcctatc
    12541 attttgaggg tggcttcgcc gccgccgaac agggtcggga agaaggtttg gactttgctg
    12601 ttgacggcat cgaaggtttc tttgaaacgc gctttggttt tatcgtcgat ttgggcgatg
    12661 gcttcttcca aaagggtgat ggcggcctgc acgtcttcgc tttggctgcg gtagtagccg
    12721 tcgcgttcgc gcgcttcttc gagttcttgc agggcggcga ggttgaccgc gccgagggct
    12781 tcgatttgtt gcgagaggct gccgatgctg ctgttcaata ctttcggcga ttctttcgcc
    12841 aacgcttcga gcgcgtccaa atcggcggcg cgttcggtca ggttttgatg gtagcgtttg
    12901 gcgttaatca gggcttcctg ctgctgcaac agggcggttt gggtggctgc ctgaagctgt
    12961 ggcaactggg tttgcagggt ttgtacgcgg gcgtattgtt ccctgccctg cgcctgaatc
    13021 tgcgcgagtt tgtcctgcac ggcgatgtac tcttcatcca aagtttgtac ggcttcggtc
    13081 aactcgtcga gcttgatgtg ctgctcgtcg ttttggaact cggtttcata ggcgaggaca
    13141 agctcttgct ggcgttcctg ccagtctaga gtttgctgtt caagccgggc gatttgctgt
    13201 tggtagtttt gtttttgctg gttgagcttg tggaccgcga cttcggcaag cccgtattgg
    13261 cggttggctt ccaacagggc aagctgcgcc tgtttcagac ggccttgctg ctcttggcgg
    13321 ctgtgcgcgg tggtttgctg ctggtgttcg agttcggcgg cggcttcctg caaagtagca
    13381 atgtcgtctg aaagcccgtc ggacgcgtgt tgcaacacgg tctgctcttc cgccaactgc
    13441 gccagttcgc gctcgatgtg ttcgcggcgg atttgccctt ggttggtacg cgccagaagt
    13501 tcggcggcgc gttgctgcgc ttggctgtat tggcgcgtgt gctgctgttg ctgctgcatc
    13561 aggtttttat gctgcacttc ggacgagcgc acggcggctt ccgcctgttt gaacgcggct
    13621 tcggcggcag aaagttcggg ggcgaggctt tccagttcgg aagcgatgcc gtcgaggcgc
    13681 gctttttggg caatcaagct ttcctgcgcg ggcttggcat agagcagaac gctgacttta
    13741 tcgacctgat ggccttcggg tgtgagccag atttggtgtg cgcccaaatc gctttgatgc
    13801 gcgagggcgt agctcaaatc gggcgcgcac aatacgccgt cgagccagtg gtgcaatgcc
    13861 gtctgaaacg gcggctgcgc ttggatttgg ttcagcaatg cctgtacggg cagggatttt
    13921 ttgatgccgc cggagagacc gtctgaaagc cacgccgcct gcccctgcgg caaaggttcg
    13981 ggcggtacga aaccttgcgg cacgtcgcgg gcgtgcaggc gttcggcaag gatgacggac
    14041 agggcgtgct gccactcagc aggcgcggta atgtgttgcc acagttgcgg cgcggcggcg
    14101 tggccggttg cctgccagaa atcggctgct tcctgctgtt gcgacaggat ttgcgacaac
    14161 gcctgctgct gcgcctgcaa ggtgatatgt tgctgcttca ggctttggaa gcggtttgag
    14221 gccgtctgaa acgcctcgcg ggcggcgtgt aaggcttctt cggcggcgat gatttgttct
    14281 tcgtaatgct cttgccggct ttgcaacaag gcagcggcct cctgcgcggc ggcggtttcc
    14341 gcttcgtcgg gcaggttcag ggcttggttc tcctgtttca gacggccttt gcgctcttcg
    14401 tgcttggcaa cggtttgttc ggcatgggca agctgctgct gcttcaacgc cagttcgcgg
    14461 cggatgcggt ttgcctcgtc ctgctgggtt tggaaggcgg cattgagcgc ggtttgggtt
    14521 tcttccaatt cgggcagacg ctcttcgtgt tcggcaacct gcatcgccca ctccgccagc
    14581 tcggtttgct tttcttcgac gtataactcg ttttcttcaa gctgcacgcg gatttgctgc
    14641 tgctcttgat gaatacgctg catctgcgcc tgcgccgcct gcttgtcgcg ttcgatgcgt
    14701 tggtgcaggt tttgctgatg gcggatttgt tcttccaaac gggcaatctg ctcgcgcaac
    14761 acgccgcgtt tgttgctcaa ttcatgcacc gcctgttgct gcgactgttc ggcggtctgc
    14821 aaggcgtgta cttcgtcgtt taacgcctga acctgcgcgg cggtttcgtc ctgctgcgcc
    14881 tgcaaagact gatgctgctc ggtcgccttg tcggcggcgg caagcgattg ctgccattgg
    14941 gcgtaatcga gcaaatcctg ttgccggttc aactgcgcgg tcagggattt gtagcgttcg
    15001 gcggtttccg cctgtttctc cagcttttcc acctgacgcg ccaattcgtt ctgcaaatcg
    15061 cccaaacgct gcaaatgctc gcgcgtgtct ttcagacggc cttccgtctc cttgcggcgt
    15121 tccttatatt tggacacacc tgccgcctcc tcgatatagg cacgcaactc ctccggccgc
    15181 gcttcgatga tgcgcgaaat catcccctgc tcgataacgg cataaccgcg cgcacccacg
    15241 cccgtaccca aaaataaatc ggtaatgtcg cggcggcgca cggtctgatt gttgatgaaa
    15301 taagtcgatt cgccctgacg cgtcagctgc cgcttgatgc tcacctcggc atactgtccc
    15361 cacgcaccct gcaaactgtg gtcgctgttg tcaaacacca gctccaccga agccctcggc
    15421 gcaggacggc gcgtcgccgc accgttaaaa atcacgtcct gcatactctc gccacgaagc
    15481 tgcttcgccg aagcctcacc caacacccag cgcaccgcgt caatcacatt cgacttgccg
    15541 caaccgttcg gcccgataac cgcgacaagc tgccccggaa catgaatcgt ggtcgggtcg
    15601 gtaaaagatt tgaagccgga gagtttgatg tgggtcaggc gcataatggt cggaaaaaat
    15661 aaaaaagaag cgtattttaa cggaaatccc gccgcaccac cccatatctt gccggcaaaa
    15721 ccttaccgca tcccgcaccc gcgatgccgt ctgaagcctt cagacggcat ttttaacgcg
    15781 ccgaaccccg gttccgccaa tgcccgatgc ttggcgcggc atttgccgta ttgccccgcc
    15841 gccgggcgcg ttataatccg ccacgacatc cgcccgattt ggaaacctgc ctatgaaacc
    15901 cgcctatttc atttccgacc tgcatttgag cgaaaagcag cccgaactga ccgcgctgtt
    15961 gctgcgtttt ttacgttctt ccgccgccgg gcaggcgcgg gcgatttata ttttgggcga
    16021 tttgtttgat ttttgggtgg gcgatgacga agtttccgag ttgaatactt cggttgcgcg
    16081 ggaaatcagg aaattgtccg acaaaggcgt tgccgtgttc ttcgtcaggg gcaaccgcga
    16141 cttcctgatc ggtcaggatt tttgccggca ggcgggtatg acgctgctgc cggattactc
    16201 ggttttggac ttgttcggct gcaaaaccct catctgccac ggagacacat tatgcaccga
    16261 cgacaaggca taccaacgtt tccgcaaaat cgtgcatcgg aagcggctgc aaaaactgtt
    16321 cctgatgctg cccctaaagt ggcgcacgcg ccttgctgcc aaaatcaggc gtgtcagcaa
    16381 aatggaaaaa caggtcaagc ccgccgatat tatggatgtc aatgccgcct ttaccgcgcg
    16441 gcaggttcgc gcctttaacg cggaaaggct gatacacgga cacacccacc gcgagcatat
    16501 ccatcacgaa aacggcttta cccgcatcgt tttgggcgac tggcataacg actatgcttc
    16561 aatcctccgc gtggacgggg acggcgcggt attcgtgccg ctggaaaaat gctgaaaatg
    16621 ccgtctgaag cctttcgggc ggcttttttt acacgcccct ttcccacact ccctccgccg
    16681 catcatcacc atccttaacc cgcattaata gccattatcg ataaaatgct tgaccgatta
    16741 cataagatta cgtaaagtgt gcaaacgcat aataattggt cttaccaatt tatcttttaa
    16801 ccaaaatatc gggtgtacgg gatttgaacg tctttccaaa cctcgacgcg ccttatgatg
    16861 caaatgccta aaaaggattc gttatggcac ttttcctcag catattcccc atcgtcctgc
    16921 tgatttggct gatggtgaaa aaaaacagta tgccctccta cgtcgcactg ccgattaccg
    16981 cagtgctgat ttacgccatc aaacttttct acttcggcga tgcgggcatg ctgctcaacg
    17041 ccaccgccgc ttccggcctc gtcaaaacgc tcacgccgat taccgtgatt ttcggcgcga
    17101 ttatgttcaa ccgtatgatg gaaaccacgg gctgcattga tgtcatccgc aaatggctgg
    17161 cgaccatcag ccccaacccc gtagcgcaac tgatgattat cggctgggct tttgccttta
    17221 tgattgaagg cgcatccggc ttcggcacgc ctgccgcgat tgccgcgccg attctgatga
    17281 gcttgggctt caacccgttg aaagtggcga ttttcacttt ggtgatgaac tccgtccccg
    17341 tctctttcgg cgcggtcggt acgccgacct ggttcggttt cgcaccgctg aacctgagtg
    17401 ccgaagacat cctcgccatc ggcaggcaga ccggcgtaat gcacttcttc gcaggtttcg
    17461 tcatccccgt catcggcttg ggcttcatcg taccttggtc tgaaatccgc aaaaacttgg
    17521 gcttcgtcgc cattgccgtc ttctcctgca ccattcctta tgtcgcattg gcgatggtca
    17581 acgaagaatt cccgtcgctc gtcgccggcg cgatcggcct gatggtgtcc gtattcgccg
    17641 ccaaccaagg ctggggcttg agcaaagacc acgccaaaga cccgaatgcc gaaaaagtgc
    17701 cgttcgccca agtcgccaaa gcactcgccc ctttgggtat gctgatcggc atgctggtgg
    17761 ttacgcgcat caaacagctc ggcatcaaag gcattttgac cagcaaagaa gaatggttca
    17821 gcttccaact gccgtttgat ttgtccaaaa tcaccgtcag cgactccctg acgattacct
    17881 tcggcaatat tttcggacaa gatgtcagcg cgtcttacca aacgctgtac gtcccggctt
    17941 ggattccgtt tgtgctgacc gtttggattt gcatcctgct gtataaaacc aaattcaaag
    18001 atgcctggac gatttattcc gtaactttca atcaaaccaa aaaaccgctg cttgccctga
    18061 tgggcgcgct gattatggtt cagctgatgc tggtcggcgg cgacaattcg atggtgaaaa
    18121 tcatcggtaa ggaatttgcc gcaatggcgg gcgaacactg ggtttatttc tcgccgtatc
    18181 tgggcgcgat cggtgcgttc ttctccggct ccaacaccgt gtccaacctg actttcggcc
    18241 cgattcagca gcaaatcgcc ttggataccg gcctgtccgt taccctgatt ctggcgttgc
    18301 agtccgtcgg cggcgcgatg ggcaatatgg tgtgcctcaa caacatcatc gccgtatgta
    18361 ccgtattgga tgtgaaaaat tccgaaggtg cgattatcaa gaaaaccgtt atcccgatgg
    18421 cgatttacgg cgtgattgcc gtcgtcgcgg caatgatttt cttcctctag gcgaaacgcc
    18481 accatccata gcggcagcac gcggggaacg gaaaccgccg ttccccgtct gggcggcgcg
    18541 tcgggcagaa tcccggtgcc ggaacaagcc cgccccgcca aacaaatgcc gtctgaaacc
    18601 aaaaaaggct tcagacggca tttttccgcc gtttgcgttc aggcgtaatg atgatgcggc
    18661 atcttgccgt caaggcggta gcgcgtgccg caatacgggc aggcaacgct gcccgattct
    18721 ccttcgcaca aaggcaaaaa cactctcgga tgcccgttcc actgctcgtt gtcgggtccc
    18781 gagcaataca gcggcagatt ttccggcaac acggaaattt cctgcggatt cagattgtcc
    18841 atttgatttt cctttgcgtg gttggcgtgc gcctatttta cgccatcggc aggctaaagg
    18901 atattttcgg cgcaaagccg caatccgcta taatccccac ttttcagacg gcataccatg
    18961 actgcgctta cccttccgga agacatccgc caacaagagc catccgccct gctctatacc
    19021 ctcgtttccg cctacctcga acacaccgcc caaaccggcg acgaatccct ctcctgcctg
    19081 tccgacgacc agcacacgct gaccgcattc tgctacctcg acagccaagt cgaagaaggc
    19141 ggcttcgtgc aactcatcgc ctccggttac ggcgaatata ttttccgcaa cccgcttgcc
    19201 gacagcctgc gccgctggaa aatcaaagcc gtgccgaaag tcttggacaa agccaaagcc
    19261 ctctacgaac aacacggcaa aaccatcgaa acgctcgccg acggaggcgc agacatcgaa
    19321 tccctgcgca aacagttccc cgactttgaa gaatgggacg gcgcatacta cgaagccgcc
    19381 gaacaagacc tgcccctgct tgcagaacac atacagtcaa actgggaaac cttcgcccat
    19441 atcgggcagg cgtgattgcg tctgtttcca gcccgtgtaa aacagcgtaa aatcggcaaa
    19501 cccgaatgac cttccgtccc catcaaggag caagctatgt tcttcaagca catcgaagcc
    19561 gcccccgccg atccgattct cggtttgggc gaagcattca aagccgaaac ccgccccgaa
    19621 aaagtcaacc tcggcatcgg cgtttataaa gacgcatccg gcgcgacacc cctcgtcaaa
    19681 gccgtcaaag aagccgaaaa acgcctgttg gaaagcgaaa ccaccaaaaa ctacctcacc
    19741 atcgacggcg ttgccgacta caacgcgcaa acccaaatcc tgctgttcgg caaagaccac
    19801 gaaatcatcg ccagccgtcg cgccaaaaca gcgcaaagcc tcggcggtac gggcgcattg
    19861 cgtattgcgg ccgagtttgc caaacgccag ttgaacgcgc aaaccatctg gatttccaat
    19921 ccgacttggc ccaaccacaa cgccatcgcc aaagcggtcg gtatccaaga caaaccttat
    19981 cgttactatg atgccgccaa acacggtttg gattgggacg gcatgattga ggacttgagc
    20041 caagcgcaaa aaggcgacat cgtcctgctg cacggctgct gccacaatcc taccggcatc
    20101 gaccctacgc ccgaacaatg ggaaactctg gcaaaacttt ctgccgaaaa aggctggctg
    20161 ccgctgtttg actttgccta ccaaggcttc ggcaacggtc tggaagaaga tgcctacggc
    20221 ttgcgcgtgt tcttgaaaca caatacagaa ttgctgattg ccagctctta ttccaaaaac
    20281 ttcggtatgt acaacgagcg cgtcggcgcg ttcactttgg tggccgaaga tgaagcaacc
    20341 gccgcccgcg cccacagcca agtcaaaacc atcatccgta ccttgtattc caacccggct
    20401 tcacacggcg cgaacactat tgcgctggtg ttggaaaatg atgatttgaa agcacaatgg
    20461 attgccgaac tcgatgaaat gcgcggccgc atcaaagcca tgcgccaaaa atttgtcgag
    20521 ttgctcaaag ccaaaggcgc aagccaaaac tttgatttca ttatcgaaca aaacggtatg
    20581 ttctccttca gcggcttgac tcccgaacaa gtcgaccgcc tgaaaaacga gtttgccatt
    20641 tatgccgtcc gctccggccg catcaacgtc gccggcatta ccgacgacaa catcgattat
    20701 ctgtgcgaaa gcatcgtgaa agtactgtaa aaagcacagc catatgaaaa tgccgtccga
    20761 accttaacca gttcagacgg catctgtttt ttgttaaaaa acaataaacc ataacaattt
    20821 caaacaagct aaaaacatct ataaaatcaa cccaatatga tgaactcaaa taaaaaatac
    20881 gaatatcatc attctcaaat tcaaaatttg aacgccacag tattatccga agccacaaaa
    20941 acctttccgc cgtcattccc gcgcaggcgg gaatccggga atccaatgct gcaagaattg
    21001 attagaaaca actgaaaccg agcaaactgg attcctgcct gcgcgggaat gacgaaataa
    21061 ggcgagtaaa actgatgaaa caaattaatt aaaaacaata acttatatca ttttctttaa
    21121 attttcgata aaatcaattt tccgtattca ataccgcccc aatcaattcc tgcgcaccgt
    21181 cgtccgccaa tttcttagcg actgcgcgtc cgagcgcgtc ggcatattcg gcaggggctt
    21241 gcgcgtccgc ccgcaacaca accgaaccgt cggggtgtcc gaccaagccg cgcaaggtca
    21301 gcaagccgtt ttcttccgtg caatatgcgg ccaaaggcac ttggcagctt ccgcccaaag
    21361 cgcgtgcgag ggcgcgttcg gcggtaacgc aggcattggt aacgccgtgg ttcaagggtt
    21421 tcaaaacttc atacaaatct tcgcggtgcg cggcaatttc gatacccaat gcgccttgtc
    21481 cggcggcagg caggctgtcg gattccgaca aaatcatgcg gatgcgtccg tccaatttca
    21541 gacgctgcaa accggcggca gccaagataa ttgcgtcgta ttcgccgtta tcgagtttgg
    21601 acaaacgggt ttgcacattg ccgcgcaaag gtttgataag caaatgcgga tagcgcgcac
    21661 gcaactgggc ttcgcggcgc aggctggatg tgccgacaac cgcgccttcg ggcatttctt
    21721 ccaaacgcgt gtattggttg gacacaaatg cgtcaaacgg attggcgcgt tcgccgatgg
    21781 tcgcaagcgc gaaaccttca ggcaaatcca tcggtacgtc tttaatcgaa tgcaccgcca
    21841 aatcggcgcg cccgtcatat aaagcctgtt ccaactcttt gacaaacaag cctttaccgc
    21901 cgacttttga caaagttttg tccaaaatct gatcgccgcg cgtggtcatg cccaaaatct
    21961 cgacttcgca atcgggatac agagccttca gacggccttg gatatgcttt gcctgccaca
    22021 tagcaagcag gctttcgcgg ctggcgatga cgagtttttt cgggttcatg ttcaatgcgt
    22081 tcaaagaatg gaaacggcgt aaagtctatc atattttgaa cgtttcttca gacggtatgc
    22141 cgtggtctat gttatattta cgcccacaaa acacgaaacc cgccacaatg aaacgcgcca
    22201 aaaaataccc tttcttaacg ttgcagcggc aacgtttcca tttgaacttt gaaaacgcct
    22261 cttccgccgc cggcatcccc gccgaacgtg atttctaccg ctgggcatgg tctgccttga
    22321 aaaatgaata ccgccgcgcc gacatcagcc tgattcttct ggacgaagaa gaagcccgag
    22381 cctacaaccg cgactaccgc ggcaaagatt acgccaccaa tgtattgagt ttcgcgctca
    22441 acgaaggcga aaccctgccc ggtcaagttt cggaaaaact gtacggcgat ttgattatct
    22501 gcccgcaagt cgttttaaaa gaagccgccg aacaaggcaa aacgcccgaa cggcattttg
    22561 cccacttaac catacacggc gttttgcacc tgatgggcta cgaccacatc gaagacgatg
    22621 aagccgaaat aatggaagcc gaagaaatcc gcctgatgcg ggcggcaggc ttccccaacc
    22681 cctaccaaga ggacggacat taaaatggac ggcgcacaac cgaaaacgaa tttttttgaa
    22741 cgcctgattg cccgactcgc ccgcgaaccc gattccgccg aagacgtatt aaacctgctt
    22801 cggcaggcgc acgagcagga agtatttgat gcggatacgc ttttaaggtt ggaaaaagtc
    22861 ctcgattttt ccgatttgga agtgcgcgac gcgatgatta cgcgcagccg tatgaacgtt
    22921 ttaaaagaaa acgacagcat cgagcgcatc accgcctacg ttatcgatac cgcccattcg
    22981 cgcttccccg tcatcggcga agacaaagac gaagttttgg gcattttgca cgccaaagac
    23041 ctgctcaaat atatgtttaa ccccgagcag ttccacctca aatccattct ccgccccgcc
    23101 gtcttcgtcc ccgaaggcaa atcgctgacc gcccttttaa aagagttccg cgaacagcgc
    23161 aaccatatgg cgattgtcat cgacgaatac ggcggcacat ccggcttggt cacctttgaa
    23221 gacatcatcg agcaaatcgt cggcgaaatc gaagacgagt ttgacgaaga cgatagcgcc
    23281 gacaatatcc atgccgtttc ttccgaacgc tggcgcatcc atgcagctac cgaaatcgaa
    23341 gacatcaaca ccttcttcgg cacggaatac agcagcgaag aagccgacac cattggcggc
    23401 ctggtcattc aagagttggg acatctgccc gtgcgcggcg aaaaagtcct tatcggcggt
    23461 ttgcagttca ccgtcgcacg cgccgacaac cgccgcctgc atacgctgat ggcgacccgc
    23521 gtgaagtaag caccgccgtt tctgcacagt ttaggatgac ggtacgggcg ttttctgttt
    23581 caatccgccc catccgccaa acataaaaat gccgtctgaa gccggaaaac aggttcagac
    23641 ggcattttgc ttatccgctt attttttgct caaagtcagc cccgtccagc cgaataacag
    23701 ggtcaaagcc aaactcaaat agcagaaaaa ggcatagggc agatattccc agaccggcac
    23761 acccagcgca tggctgataa atacgccgca cacgctccac ggaacaagcg ggttgatgac
    23821 cgtccccgca tcttcgagcg tccgagacag gttgcgcgaa tgcagaccga gcttatcgta
    23881 aacgggtttg aacgtttcac ccgacaacaa aatactcaaa tattgctcgc caatcaggaa
    23941 attaaccccg accgaagtca tagcaacgct gaatgtggca cgcccggcat tcgtcaaaaa
    24001 gctgcggacg gcatccagca gggaaggaat cgcgcccagt gcaaacaaca gcccgcccaa
    24061 actcatcccg aggataacga tagtctgcgt gaagaacata ctttccaaac cgccgcgcga
    24121 aatgagtttg gcaatgtctt taaacgcttc gccttcgagt ttgtagccgc cgtaaaacca
    24181 cgcgccgagc tgacgcagat cgggcgtact gtgcagatac gttacggcaa cggcaaccat
    24241 gacggtaaag agcatggcga ccacggcatt gacgcgcatc aatgccaaaa cgaccaacag
    24301 tgcaaacgga atcagcgaat agccgtgcac caatcccgtg gcttcaagct ggctgcggaa
    24361 ggattcgacg ctgttcaaat cctgcgcggc gacactggga agaagccaca gcatcagtgc
    24421 cgcactgatg agccacgcgg gaatggtggt gtacatcata tttttgatgt gctcaaacag
    24481 gtcgatgccg acgatggacg cggaaatgcc cgtggtgtcg gaaagcgggg acattttgtc
    24541 gccgaaaaac gcaccggaaa caatcgcgcc cgccgtcatc gccatatcgg catgaaacgc
    24601 cgccgccatc cccataaagg caacgccgac ggtggcgcag gtggtcaggc tgctgccgat
    24661 ggacacgccg atgacggaac acagcgcgaa agcggaaaaa taaaaataag tcggggaaat
    24721 cagcccgaaa ccgtaataca tcagcgtcgg aatcgcaccg ctcatcatca gcgcgctgac
    24781 catcagcccg atgaagaaaa acaggtaaat cgcgcccata ccctgattca acgcgcctat
    24841 catgccctgc tgcatatcgt tgtatttcaa accgcgcgcc aagccgtaca aaatcagcac
    24901 gacgatggcg gcaataatgg acatatgcgg cagccactcc aatgaaatga tggtatagcc
    24961 catcgcggca atcagagcga cgacgacggc aagtgcctca ccgcgcggca tatcgagtaa
    25021 ggatttgaaa gcaaacatga ttctttgccc gatttgatat aaagttaacg gattctaaca
    25081 caccataccg aatccgccca acgttttcag acggcatttt agcgttttac aaaagccgga
    25141 cagaaacaaa acggcaattt tctcaattta aattaatttt tttgaaatat aattttttat
    25201 ttttttgttt gttggcatat aagactttta tggattgaag tattaaaacc tcggcacaca
    25261 tgccgcaaac accggtacgc aaaaatgccg tctgaaagat gttcagacgg catttctcaa
    25321 gaaaaagatt atttgtccgc cctatatccg tacaccgcaa aaaacacgat atacagataa
    25381 cagatgacgg aaacgacaaa cgaagacatc agggtgtaag tatctgccac ccagccctgc
    25441 acgacaggaa cgaccgcacc gccgacaatc gcggtacaca gtacaccgga agccgcgttg
    25501 gtaaattttc ccaatccttt ggttgccaaa gagaaaatcg tcgggaacat aatcgagttg
    25561 aaaaaaccga tggcaagcag cgaccacatc gccacatccg cattgccgct gcccgtcgcc
    25621 atcgcgacgg caagcagtac gaccgcagcc gatgcgttaa acgccaaata acggttgggc
    25681 gcgaatttcg ccatcaccgc cgaaccgagg aaacgtccga ccatcgcgcc gccccaatag
    25741 aacgacaggt aatgcgcggc agaagcatga tccagccctt tcagataacc caatacgttg
    25801 accatcaacg aaccgataga cacctccgcg ccgacatagc agaaaatgcc tgccgtaccg
    25861 aacacgagat gtttgtattg ccatacgctg gttttgccgt cgtggttgtg cgcgctttcc
    25921 tcggcggcaa ttttgcgcgc gtcgggcagc cggatcattt tcacgaaaac ggcaaggata
    25981 atcagcagcc ccgccagtcc caaataggga atctgtacgg aagaaatctg ttccgccttg
    26041 ctgacggttt gggttgcgtc cgccaaaatc aggaacgcgc cgatttgcgg cgcaatggtc
    26101 gtacccaaag cgttaaacgc ctgaaccagc gtcagtgttg ccgattcctt gccgggtttc
    26161 gccagcaggg taacataagg attaccggcg acctgaagca gcgttacgcc ggaagccaaa
    26221 ataaacaacg cgcccaaaaa taccgcgtaa gaatggctgc ccgcagcagg ataaaacagc
    26281 aggcatccga ccgccgtcag cagaaagccg ccgataacgc cgtttttgta gccgactttg
    26341 ccgacaaaag cccccatcgg gatggacatc accgcatagg cagtaaagaa acagaattgg
    26401 atcagcatcg cctgaacgta agacaggtcg aaaatttctt tcaaatgagg gataaggatg
    26461 tcgttcatgc aggtaataaa acccatcatg aagaacagcg tggtcaagac gaccaatggg
    26521 gacgtatgat tgttttgtga ttgcgcagac atgatttgcc tttcgtggtg tcggaatatt
    26581 acccgtttca agaaaataga aattgacatt tcttaacggc aatgcaaatc atactgatat
    26641 ttgaacattt tgcaaacttt atcggatgca ggcaatgcgc cgatctgaaa ttcaagcagc
    26701 agaaattaat attttacagt tgcttgcata gattttaata gattttaaaa aattccggcg
    26761 gacttgcaaa aaccgttccc aataaaatga aaatccgccg ccgtatccgg aaaattcgga
    26821 aatgccgcat cgtggcatca aaaccggagc ggaaagaatg tcgtatgccc gacaaaaaag
    26881 gggcatacga ctgtgccgta ttttttgcac cgtcctccgc ccttcggcgg gggcgtgaaa
    26941 atatagtgga ttaacaaaaa tcaggacaag gcgacggagc cgcagacagt acaaatagta
    27001 cggaaccgat tcactcggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
    27061 aggcaacgcc gtactggttt ttgttaatcc actataccgt acaatccgtc gccgattttt
    27121 gcctgtttga gactgtttgc aatgataccc tcctacacct acgccgccct cgccttttcc
    27181 gccttcactt ccgccaccct cctgcccggc acatccgaag ccgcctttgc cctgttcgtc
    27241 cgccgctttc ccgaacacgc atacggcgcg ttgctgtgcg ccggccttgc caacggattg
    27301 ggcagtatgg tttcctattg gatgggacgt ttgctgcctt cccgaaaaat gccgtctgaa
    27361 aaaacactga atctgatgcg gcgtttcggt atttggctgc ttccgtttgc ctggctgccc
    27421 gtcgtcggcg acgcactgcc gcttgccgcc ggctggctgc ggttgaatcc gtggacaagc
    27481 gggctgatgc tggttatcgg caaaacggcg cgttacgcct tcattctgtg gggaatgcaa
    27541 tattacgccg cctgaaccgt gcgttataat gccgaccgca caaaccgact gcacaaaaaa
    27601 atgaacagac acatccgcca agaaattttc gaacgcttcc gcgccgccaa cccccatccg
    27661 accaccgagc tgaatttcaa ctcccctttc gagcttttaa ttgccgttct gctttcggcg
    27721 caggcgaccg atgtcggcgt aaacaaggcg acggcgaagc tgtttccggt tgccgatacg
    27781 ccgcaggcga tgctggattt gggtttggac ggcgtgatgg aatatacgaa aaccatcggg
    27841 ttgtataaaa ccaagtccaa gcacatcatg cagacctgcc gcatcctgct ggaaaaatac
    27901 aatggcgaag tgccggaaga ccgcgaggct ttggaatcgt tgccgggcgt ggggcgcaaa
    27961 acggcaaacg tggtattgaa cacagcgttc ggacaccccg ttatggcggt cgatacgcat
    28021 attttccgcg tgtccaaccg aaccaaaatc gcacccggga aagatgtgcg cgaagtcgaa
    28081 gacaaactga tgcgcttcat tcctaaagaa tttctgatgg acgcgcacca ctggctgatt
    28141 ttgcacggac gctacacctg caaggcactc aaaccgcaat gccaaacctg catcatcaac
    28201 gatttgtgcg aatatcccgc caaagcctga ctgccgccgc ccttcaaaat gaaacgcttg
    28261 aaaattcgga cggacgttgt agaatttcaa cctatgccgt ctgaagccga caaaatgcgc
    28321 ttcagacggc attaaagccg gtaccggcat ccgacaaagg acaaaacgtg ttcaaaaaat
    28381 accaatacct cgctttggca gcactgtgtg ccgcctcgct ggcaggctgc gacaaagccg
    28441 gcagcttttt cggtgcggac aaaaaagaag catccttcgt agaacgcatc gaacacacca
    28501 aagacgacgg cagcgtcagt atgctgctgc ccgactttgt ccaactggtt caaagcgaag
    28561 gtccggcagt cgtcaatatt caggcagccc ccgccccgcg cacccaaaac ggcagcggca
    28621 atgccgaaac cgattccgac ccgcttgccg acagcgaccc gttctacgaa tttttcaaac
    28681 gcctcgtccc gaacatgccc gaaatccccc aagaagaagc agatgacggc ggattgaact
    28741 tcggttcggg cttcatcatc agcaaagacg gctatattct gaccaatacg cacgtcgtta
    28801 ccggcatggg cagtatcaaa gtcctgctca acgacaagcg cgaatatacc gccaaactca
    28861 tcggttcgga tgtccaatcc gatgtcgccc ttctgaaaat cgacgcaacg gaagagctgc
    28921 ccgtcgtcaa aatcggcaat cccaaagatt tgaaaccggg cgaatgggtc gccgccatcg
    28981 gcgcgccctt cggcttcgac aacagcgtga ccgccggcat cgtgtccgcc aaaggcagaa
    29041 gcctgcccaa cgaaagctac acacccttca tccaaaccga cgttgccatc aatccgggca
    29101 actccggcgg cccgctgttc aacttaaaag gacaggtcgt cggcatcaac tcgcaaatat
    29161 acagccgcag cggcggattc atgggcattt ccttcgccat cccgattgac gttgccatga
    29221 atgtcgccga acagctgaaa aacaccggca aagtccaacg cggacaactg ggcgtgatta
    29281 ttcaagaagt atcctacggt ttggcacaat cgttcggttt ggacaaagcc ggcggcgcac
    29341 tgattgccaa aatcctgccc ggcagccccg cagaacgtgc cggcctgcag gcgggcgaca
    29401 tcgtcctcag cctcgacggc ggagaaatac gttcttccgg cgaccttccc gttatggtcg
    29461 gcgccattac gccgggaaaa gaagtcagcc tcggcgtatg gcgcaaaggc gaagaaatca
    29521 caatcaaagt caagctgggc aacgccgccg agcatatcgg cgcatcatcc aaaacagatg
    29581 aagcccccta caccgaacag caatccggta cgttctcggt cgaatccgca ggcattaccc
    29641 ttcagacaca taccgacagc agcggcggac acctcgtcgt cgtacgggtt tccgacgcgg
    29701 cagaacgcgc aggcttgagg cgcggcgacg aaattcttgc cgtcgggcaa gtccccgtca
    29761 atgacgaagc cggtttccgc aaagctatgg acaaggcagg caaaaacgtc cccctgctgg
    29821 tcatgcgccg tggcaacacg ctgtttatcg cattaaacct gcaataaccc acataattta
    29881 accaaaatat tttttaaatg ataaaatgcc cgtcaatacc gattaacggg cattttacaa
    29941 aaatcagttg gttgatttta aacaaatatt ttttacgaca aaaaatattt ccgcccattt
    30001 tgccaacaaa acccaaaccg gaggcggaca gatacgctcc catccggacg cttaacaccc
    30061 ccataaccat gcagctgatc gactattcac attcattttt ctcggttgtg ccaccctttt
    30121 tggcactggc acttgccgtc attacccgcc gcgtactgct gtctttaggc atcggtattc
    30181 tggtcggcgt tgcctttttg gtcggcggca accccgtcga cggtctgaca cacctgaaag
    30241 acatggtcgt cggcttggct tggtcagacg gcgattggtc gctgggcaaa ccaaaaatct
    30301 tggttttcct gatacttttg ggtattttta cttccctgct gacctactcc ggcagcaatc
    30361 aggcgtttgc cgactgggca aaacggcaca ttaaaaaccg gcgcggcgcg aaaatgctga
    30421 ccgcctgcct cgtgtttgta acctttatcg acgactattt ccacagtctc gccgtcggtg
    30481 cgattgcccg ccccgttacc gacaagttta aagtttcccg cgccaaactc gcctacatcc
    30541 tcgactccac tgccgcgcct atgtgcgtgc tgatgcccgt ttcaagctgg ggcgcgtcga
    30601 ttatcgccac gcttgccgga ctgctcgtta cctacaaaat caccgaatac acgccgatgg
    30661 ggacgtttgt cgccatgagc ctgatgaact attacgcact gtttgccctg attatggtgt
    30721 tcgtcgtcgc atggttttcc ttcgacatcg gctcgatggc acgtttcgaa caagccgcgt
    30781 tgaacgaagc ccacgatgaa actaccgttt cagacgctac caaaggtcgt gtttacgcac
    30841 tgattattcc cgttttggcc ttaatcgcct caacggtttc cgccatgatc tacaccggcg
    30901 cgcaggcaag cgaaacattc agcattttgg gggcatttga aaataccgac gtgaacactt
    30961 cgctggtatt cggcggcact tgcggcgtcc ttgccgtcgt tctctgcacg ctcggcacga
    31021 ttaaaaccgc cgattatccc aaagccgttt ggcagggtgc gaaatctatg ttcggcgcaa
    31081 tcgccatttt aatcctcgct tggctcatca gtacggttgt cggcgaaatg cacaccggcg
    31141 attacctctc cacactggtt gcgggcaaca tccatcccgg cttcctgccc gtcatcctct
    31201 tcctgctcgc cagcgtgatg gcgtttgcca caggcacaag ctgggggacg ttcggcatta
    31261 tgctgccaat tgccgccgcc atggcggtca aagtcgaacc cgcgctgatt atcccgtgta
    31321 tgtccgcagt aatggcgggg gcggtatgcg gcgaccactg ttcgcccatc tccgacacga
    31381 ccatcctgtc gtccaccggc gcgcgctgca accacatcga ccacgttacc tcgcaactgc
    31441 cttatgccct gacggttgcc gccgccgccg catcgggcta cctcgcattg ggtctgacaa
    31501 aatccgcgct gttgggcttt ggcacgacag gcattgtatt ggcggtgctg atttttctgt
    31561 tgaaagataa aaaacgcgcc aacgcctgac ccctttccct gttcagacgg catatgccgt
    31621 ctgaagcttt ttggagcaaa ccgaaatgac tgttccctgt attccgcgcg gcctcgtcat
    31681 ggccgacatt gccgccttcc gcctgaccga agaggaaaag caacgccttc tcgatcccgc
    31741 cgtcggcgga gtcatcctgt tccgccgcaa cttccaaaat atcgcacagc tcaaagaact
    31801 caccgccgaa atcaaagccc tgcgcacacc cgaactcatt atcgccgtcg atcacgaagg
    31861 cggcagggtg caacgtttca tcgaaggctt cacccgcctg cccgcaatga gtacgctcgg
    31921 cgagatttgg gacaaagacg gcgcgtccgc cgccgaaacc gccgccggac aagtcggccg
    31981 ggttttggca accgagcttt ccgcctgcgg catcgatttg tccttcacgc ccgtcttaga
    32041 tttggactgg ggaaactgcc ccgtcatcgg caaccgcagt ttccaccgca atcccgaagc
    32101 ggtcgcccgc cttgccctcg cccttcaaaa aggtttggaa aaaggcggta tgaaatcctg
    32161 cggtaaacat tttcccggac acggatttgt cgaaggagac agccatttgg tgctacccga
    32221 agacgggcgc agcctgtccg aactcgaagc cgccgacctc gcccccttcc gcattatgag
    32281 ccgcgaaggt atggcagcgg ttatgcccgc ccacgtcgtt tatccgcaag tggacacaaa
    32341 gcccgcaggg ttttccgaaa tctggctcaa acaaattttg cgccgcgaca tcgggtttaa
    32401 aggcgtaatt ttctcggatg atttgactat ggagggcgcg tgcggggcag gcggcatcaa
    32461 agaacgcgcc cgcatttcct ttgaggcagg ttgcgacatc gtcctcgtct gcaaccgtcc
    32521 cgaccttgtg gacgaactgc gcgaagattt ccgcattccc gacaatcccg ctttggcgca
    32581 acgttggcaa tatatggcca acacgctcgg cagtgccgcc gcgcaagccg tgatgcaaac
    32641 aacggatttc caagcggcgc aagcctttgt tgccggcctt gcctcgccgc aggacacggc
    32701 gggcggcgtg aaagtcggag aggcctttta aatccgcccg acacgcggca aaaaggcaaa
    32761 acggtatagg cacgccgtat tttttgcgct ataatcacgc cttacttaat tcggaatgta
    32821 gcgcagcccg gtagcgcact tcgttcggga cgaaggggtc ggaggttcga atcctctcat
    32881 tccgaccata ttcaaaccgc ctcggcatgg attgccggga cggtttttcc ttttggcggc
    32941 aacagaaaaa tgccgtctga aacagaatgc tgtttcagac ggcatttcgt tttcccccaa
    33001 ccctgccatt tgcgcgcact ggcacagagt atcggcaagg ttcaaaagac catcggaatt
    33061 taggaagccg ggcttgtacc caacgacggc atcttattca gtaaggctcc gcccccaatc
    33121 aggcagcgga aatgccggca gtttcacgcg gaagcggcac actttccccg tatgtcaaac
    33181 atcggcaaaa cggtaagccg gccctttgag ttacactgct taaactactc tatccccgcc
    33241 catttgtgcg tctgcacgct caactgccaa tgcaccggcg cgtcggggcg gctgttcaga
    33301 ataccgattt ggcggatggt gtcgtggatg ttcatcgcac cgtcttgctc acagggcgaa
    33361 aggtagtaat gatgtgcgcg gattttgcgt tccatgtttt cgcagaacgc aaggacatca
    33421 ccatcggcaa caatccgcac ttcgtcggct gtttcgatac agctattttc atatttggcg
    33481 gcatagcagg ctttggggct ggtggcgacg tagtcgattt gcggcggcgc gggattgagt
    33541 ccgttggttt cgagacagag gaaatagcct tccgccttga gcgtgtccag cagcatatcg
    33601 agatgcggct gtatggtcgg ttcgccgccg gtgatgatga tgttgcgggc ggcataggtt
    33661 ttcagacagc ctaagatgtc ggacaggctc atcatgccga atgtcaaata atcggtatca
    33721 caccagccgc aggcgaggtt gcatttgccc aagcggacga aaacggcggg catgcccgtg
    33781 ttccagcctt cgccttgcag gctttcaaag atttcgacga tgcggtattg gggattttcg
    33841 ggggcaacat tgattttttt cataccgcca acaccgccgc cgctgccaaa aatgcgggca
    33901 aaccttgttt gacgagtatg cctttgttgc cggaagagaa cgcgccccac gcggcggcaa
    33961 tcaggacgaa gccgagaaac agtaccattg cgccgtacac cgcgttatcc ggcgcggcaa
    34021 accgcgacca caccagcccg atgccgagaa agccgttata cagcccttgg ttgccgaaca
    34081 gggtctgcac gcgcttttgt tccataaatt cataaggcag cttgaatatt tccgccgctt
    34141 tttcgctggg aatctgcgtc atttcaagcc aggcgatgta gaaatgttcg acggcgacga
    34201 agaggactag gaggatgaag aggagtttca tgggtattcc ttttaatggg tgtatttgat
    34261 atggaaggtc gtctgaaaaa acggggaatc cgtacaggag aaaaaaccgt tttacggtaa
    34321 attcaaataa ggacgctcta aaccgtcctt tccgcgcagg cgggaatcca gagagttgga
    34381 attgctgcaa tttttaaata ttcctgaaaa accaaggtct agattcccgc ctgcgcggga
    34441 atgacggctt agggcaagtt ttattcaaat tcactatatc gatacgttca aactatcccc
    34501 tacccccctt catactccgc acacgatgtc ggcgtttccc acaatttgac gcggcacacg
    34561 ttcagccccg cgtttttcag acggtcgtac atttcgaccg ccatattttc ggcagtggtg
    34621 cggcagggca ggcgcagggt tttcatgttc cagccttcca aaagtgcggc gatttgagat
    34681 tcgcgcccgt tgccgccgtg gtagatgaag gcgtggtcga aggggtcggt aatgtgttgt
    34741 ttgacaatgg ctttcaagtc ggtaaagtcc atcaccatac cgtctttcgg cccgcctctg
    34801 acggggtcgt ctgaaacggt gatttcgagt ttgtaggtat gcccgtgcag gttttggcat
    34861 ttgccgtcat gcccgtcgag catatgcgag gagtcgaagg tgaagatttt ggtgattttc
    34921 atgggttctc aaatgggcaa atagccttga aagccgattt cggcgggttt gccgtgcgga
    34981 ttcggggtca ggcggatatg gaaattgccg atgcggtcga gcgcaagcag gttgtccacg
    35041 tcttttaagt tgtctttatg ttgctgaaca tagctgtcgg tcaacagggt ttgcagggct
    35101 ttttgtttgg cttcggcagg cgtggcggcg gcgaacagcc caaaagcgtg cgcttcggcg
    35161 agctgccctg cgcgataacc gccgacattg gcaaaataca ggcgcggggc gtgttcagac
    35221 ggcattttcg ttttgggcgt ttcggatacg gcgatgtcgt aaccgtccgc ccattcgaca
    35281 atctgccagc cgtcgatgtg gattttgtcc gcatcgccga accacgcggc tttgagtgcg
    35341 gggacggctt cacggtagtc gtcgcacacg gcaaattgga tgtcgtgcac ttcgatattc
    35401 gacctgccgg cattgccgcc gaggtaaaac atatggagct tgggcatatc gtattcctta
    35461 tggtttgcgc gtgttcagac ggccttttta ctttccagat attccgccag cccgcgttcg
    35521 cgcaagatac agctcgggca ttcgcggcag ccgccgccga cgatgccgtt atagcaggtg
    35581 tgggtttgct cgcggatata gtccagcgcg cccatttcgt ccgccaacgc ccaagtttgc
    35641 gccttggtca gatacatcag cggcgtgtgg atttgaaaat catagtccat cgccaaatta
    35701 agggtaacgt tcatcgattt gacaaacacg tcgcggcagt cgggatagcc ggagaagtcg
    35761 gtttcgcaca cgcccgcgat gatgtgccgt atcccctgcc ctttggcgta aatcgcggca
    35821 tagagcagga aaagcgcgtt gcggccgtct acaaaggtat tcggaacgcc gttttcggca
    35881 gtttcgatgg cggcggtatc gtccatcagg gcattgtgcg taatctgccg catcaggctc
    35941 aagtcgagta cggtttgttt gatgcccaaa tcctgcgcaa tccagcgggc gcgttccagc
    36001 tcgacggcat ggcgttgccc gtattggaaa gtgatggctt ggacgttttc gcgcccgtag
    36061 gtttggattg cctgaatcag gcaggtggtc gaatcctgac cgcccgaaaa gatgaccaag
    36121 gcttgttggt ttgacatatc gaatcctagt tttggtaacg cgggaggttt gcgaaccgcg
    36181 aagttgaaca gtaaagatgc ggattataca ggcaaacgcg ttcagacggc atctgaacgc
    36241 catgccgtct gaacacgcgg cttatgccga cttgacggcg agttccgcca agcgtttgct
    36301 ttgtgcgaag gcgatattgt tttcttcggc ggtcaggacg ggagccgctg tcgggaatat
    36361 cttttttgac ggcttcgcag acggcggaga ctttggggac ggtgcgcaat acggcttcgc
    36421 aaccttcaac gctgtcgatg ccgcgagcga ttcggcgggc gggattgcgg gggctgccgt
    36481 tttgggaata gtagaggacg aggattttga gggggatttg ggttcatggg cggattccgt
    36541 taaaatggtt cggtcttcaa acggcataac agttgttccg atgccgtctg aaacagccgc
    36601 agttcaggca tttgttcctg acggcaaaat tggctataac atcgggcatc aatgcccaac
    36661 ttactgaccg ggcaagaaat tatgaccttt ttacaacgtt tgcaaggttt ggcagacaat
    36721 aaaatctgtg cgtttgcatg gttcgtcgtc cgccgctttg atgaagaacg cgtaccgcag
    36781 gcggcggcaa gcatgacgtt tacgacgctg ctggcactcg tccccgtgct gaccgtgatg
    36841 gtggcggtcg cttcgatttt ccccgtgttc gaccgctggt cggattcgtt cgtctccttc
    36901 gtcaaccaaa ccattgtgcc gcagggcgcg gacatggtgt tcgactatat caatgcgttc
    36961 cgcgagcagg cgaaccggct gacggcaatc ggcagcgtga tgctggtcgt tacctcgctg
    37021 atgctgattc ggacgataga caatacgttc aaccgcatct ggcgggtcaa ttcccagcgt
    37081 ccgtggatga tgcagtttct cgtctattgg gctttactga cgttcgggcc gctgtctttg
    37141 ggcgtgggca tttcctttat ggtcggctcg gtacaggatg ccgcgcttgc ctcaggtgcg
    37201 ccgcagtggt cgggcgcgtt gcgaacggcg gcgacgctga ccttcatgac gcttttgctg
    37261 tgggggctgt accgcttcgt gccaaaccgc ttcgttcccg cgcggcaggc gtttgtcggg
    37321 gctttggcaa cagcgttctg tctggaaacc gcacgttccc tcttcacttg gtatatgggc
    37381 aacttcgacg gctaccgctc gatttacggc gcgtttgccg ccgtgccgtt ttttctgttg
    37441 tggctgaacc tgttgtggac gctggtcttg ggcggcgcgg tgctgacttc ttcactctcc
    37501 tactggcagg gagaagcgtt ccgcaggggc ttcgactcgc gcggacggtt tgacgacgtg
    37561 ttgaaaatcc tgctgcttct ggatgcggcg caaaaagaag gcaaagcctt gcctgttcag
    37621 gagttcagac ggcatatcaa tatgggctac gacgagttgg gcgagctttt ggaaaagctg
    37681 gcgcggcacg gctacatcta ttccggcaga cagggttggg tgttgaaaac gcgggcggat
    37741 tcgattgagt tgaacgaact cttcaagctc ttcgtttacc gtccgttgcc tgtggaaagg
    37801 gatcatgtga accaagctgt cgatgcagta atgatgccgt gtttgcagac tttgaacatg
    37861 acgctggcag agtttgacgc tcaggcgaaa aaacagcagc aatcttgagt atttttgaaa
    37921 ctgtattttt atcctaaaga cattttccta tctgctgatt ccaccgtttc ctttctgtgt
    37981 gccgccataa tttttatgat agacaagcca ttcaagtttg cattgactgc tttttcattg
    38041 gcaatatcca aatggctggt cgctgcatct aaaaatagga ttttcggttc gcaatattaa
    38101 ggcccgcgcc aatacgatgc gttgtttttg tccgcctgac agtgcgctgc ccatatcgcc
    38161 gatcaaggtt tcatagccca taggcatggc gcagatttct tcgtgaatca tagcaaggca
    38221 ggcgcaatgt tcgatttttc ctctatccgg ttccgtatcg aaaaaacaag gggctgtact
    38281 agattagccc taaatcccac accaatcccg caggatttta agctgttgag acggtgtgcc
    38341 gaagttaaat cgaaattcgc attctttcaa gaacagcggg aaagatttac gatcgattcc
    38401 gttgtatttt cgcaagacgc gtttagtcta gagtctgtat attacattta tttttagggt
    38461 ctgctagcca atttcttgtt cccttcatta ttttatcttc tgaaagaaaa ttattttttt
    38521 ccatgctatt aatattaatg atatgatttt tatttaaaat aatttttcca tataaaattt
    38581 ccggtttgta atagcaagag tctgaagcaa atccttgccc gtcaatataa gaaaaataaa
    38641 tgttatcatc ctccttataa tattctaaaa ccaatccgga tattatttta tcaaaattcc
    38701 cttcatttcc atctttcatg actaaaatat aattagcagg ccaaccttta tcgtaaacat
    38761 attcaaactg atagttcccg aaactctcga tatccgaact aaaaaagaag aaaagcaaat
    38821 taatagccaa tacaaaaaca aataagaaca aaaatagcaa aattttcaac ttagttaaca
    38881 atatttacct ctcctttaaa ttcaatcctg aaaggtaccc cttaccctgg gctaccaatt
    38941 atagtttcca tatttctaaa tattgttttt acattacttt tttctccccc caaaggaatg
    39001 cattttaaaa tcatgctttt caggtgctaa tcgatactta ccattaacat ctttaatcac
    39061 agatatattt ccatgtatag cccaacgtga aaaatctgag tattatatac agttatacct
    39121 tactgaaaca aagctagctg tacatatctg taccatattt ttactacaaa caaaaaatat
    39181 ttataattat taaggataag ataaaaaacc ccaacgccag attaattctt aataatttca
    39241 aataaaaaat atttgagatt ttttcttttc caaataatat attgaaatat ttaaagttaa
    39301 ggtagcttga tttaagtaga aaatcaaaaa aaccattaat taatttttct tctttaaata
    39361 ttttatatga tattataaaa ttaaataaca ttgcgaaaac atatgaaatc atcaaaatac
    39421 caaatatata gtgggaatgc attattattt agctccttct tttggctttg acaaattgac
    39481 agtccattga tatccaatgc ttgcatttgc aattgctgac gcacctgccc ctgttttccc
    39541 tgttagtatc aatgattctt ttcttgcaag atgtgcacct gcacaaccac ctaatactaa
    39601 accaccacct attgtaaatc cttcct
//
LOCUS       DAWFHY010000008        37017 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.008, whole genome shotgun sequence.
ACCESSION   DAWFHY010000008 DAWFHY010000000
VERSION     DAWFHY010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 37017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.008"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            63..611
                     /locus_tag="WGQ68_000339"
     CDS             63..611
                     /locus_tag="WGQ68_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0714214.1"
                     /translation="MPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTC
                     TGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSK
                     EEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEG
                     LRFAIREGGRTVGAGVVSSVIA"
     gene            629..940
                     /gene="rpsJ"
                     /locus_tag="WGQ68_000340"
     CDS             629..940
                     /gene="rpsJ"
                     /locus_tag="WGQ68_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEZ0714215.1"
                     /translation="MANQKIRIRLKAYDYALIDRSAQEIVETAKRTGAVVKGPIPLPT
                     KIERFNILRSPHVNKTSREQLEIRTHLRLMDIVDWTDKTTDALMKLDLPAGVDVEIKV
                     Q"
     gene            1061..1366
                     /locus_tag="WGQ68_000341"
                     /pseudo
     CDS             1061..1366
                     /locus_tag="WGQ68_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1630..2274
                     /gene="rplC"
                     /locus_tag="WGQ68_000342"
     CDS             1630..2274
                     /gene="rplC"
                     /locus_tag="WGQ68_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEZ0714216.1"
                     /translation="MTLGLVGRKVGMTRVFDEQGVSVPVTVLDMSANRVTQVKSKDTD
                     GYTAVQVTFGQKKANRVNKAEAGHFAKAGVEAGRGLIEFALTEEKLAELKAGDEITVS
                     MFEVGQLVDVTGISKGKGFSGTIKRHNFGAQRTSHGNSRSHRVPGSIGMAQDPGRVFP
                     GKRMAGQYGNTKATVQKLEVVRVDAERQLLLVKGAVPGAVNSDVVVRPSVKVGA"
     gene            2274..2894
                     /gene="rplD"
                     /locus_tag="WGQ68_000343"
     CDS             2274..2894
                     /gene="rplD"
                     /locus_tag="WGQ68_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEZ0714217.1"
                     /translation="MELKVIDAKGQVSGSLSVSDALFAREYNEALVHQLVNAYLANAR
                     SGNRAQKTRAEVKHSTKKPWRQKGTGRARSGMTSSPLWRKGGRAFPNKPDENFTQKVN
                     RKMYRAGMATILSQLTRDERLFAIEALTAETPKTKVFAEQVKNLGLEQVLFVTKQLDE
                     NVYLASRNLPNVLVLEAQQVDPYSLLRYKKVIITKDAVAQLEEQWV"
     gene            2891..3205
                     /gene="rplW"
                     /locus_tag="WGQ68_000344"
     CDS             2891..3205
                     /gene="rplW"
                     /locus_tag="WGQ68_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEZ0714218.1"
                     /translation="MNQQRLTQVILAPIVSEKSNVLAEKRNQMTFKVLASATKPEIKA
                     AVELLFGVQVASVTTVTTKGKTKRFGRTLGRRRDVKKAYVSLAAGQELDLEAAAAAAD
                     KE"
     gene            3211..4044
                     /gene="rplB"
                     /locus_tag="WGQ68_000345"
     CDS             3211..4044
                     /gene="rplB"
                     /locus_tag="WGQ68_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEZ0714219.1"
                     /translation="MAIVKMKPTSAGRRGMVRVVTEGLHKGAPYAPLLEKKNSTAGRN
                     SNGHITTRHKGGGHKHHYRVVDFKRNKDGIPAKVERIEYDPNRTAFIALLCYADGERR
                     YIIAPRGIQAGAVLVSGAEAAIKVGNTLPIRNIPVGTTIHCIEMKPGKGAQIARSAGA
                     SAVLLAKEGAYAQVRLRSGEVRKINVNCRATIGEVGNEEQSLKKIGKAGANRWRGIRP
                     TVRGVVMNPVDHPHGGGEGRTGEAREPVSPWGTPAKGYRTRNNKRTDNMIVRRRYSNK
                     G"
     gene            4051..4329
                     /gene="rpsS"
                     /locus_tag="WGQ68_000346"
     CDS             4051..4329
                     /gene="rpsS"
                     /locus_tag="WGQ68_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEZ0714220.1"
                     /translation="MARSLKKGPYVDLHLLKKVDAARASNDKRPIKTWSRRSTILPDF
                     IGLTIAVHNGRTHVPVFISDNMVGHKLGEFSLTRTFKGHLADKKAKKK"
     gene            4338..4667
                     /gene="rplV"
                     /locus_tag="WGQ68_000347"
     CDS             4338..4667
                     /gene="rplV"
                     /locus_tag="WGQ68_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEZ0714221.1"
                     /translation="MRVNAQHKNARISAQKARLVADLIRGKDVAQALNILAFSPKKGA
                     ELIKKVLESAIANAEHNNGADIDELKVVTIFVDKGPSLKRFQARAKGRGNRIEKQTCH
                     INVTVGN"
     gene            4677..5369
                     /gene="rpsC"
                     /locus_tag="WGQ68_000348"
     CDS             4677..5369
                     /gene="rpsC"
                     /locus_tag="WGQ68_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEZ0714222.1"
                     /translation="MGQKINPTGFRLAVTKDWASKWFAKSTDFSTVLKQDIDVRNYLR
                     QKLANASVGRVVIERPAKSARITIHSARPGVVIGKKGEDIEVLKRDLQVLMGVPVHVN
                     IEEIRRPELDAQIIADGIAQQLEKRVQFRRAMKRAMQNAMRSGAKGIKIMTSGRLNGA
                     DIARSEWYREGRVPLHTLRANVDYATSEAHTTYGVLGLKVWVYTEGNIKSSKPEHESK
                     QRKAGRRNAAAN"
     gene            5353..5769
                     /gene="rplP"
                     /locus_tag="WGQ68_000349"
     CDS             5353..5769
                     /gene="rplP"
                     /locus_tag="WGQ68_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEZ0714223.1"
                     /translation="MLQPTRLKYRKQQKGRNTGIATRGNKVSFGEFGLKAVGRGRLTA
                     RQIEAARRAMTRHIKRGGRIWIRVFPDKPITEKPIQVRMGGGKGNVEYYIAEIKPGKV
                     LYEMDGVPEELAREAFELAAAKLPIPTTFVVRQVGQ"
     gene            5769..5960
                     /gene="rpmC"
                     /locus_tag="WGQ68_000350"
     CDS             5769..5960
                     /gene="rpmC"
                     /locus_tag="WGQ68_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009175233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEZ0714224.1"
                     /translation="MKANELKDKSVEQLNADLLDLLKAQFGLRMQNATGQLGKPSELK
                     RVRRDIARIKTVLTEKGAK"
     gene            5960..6223
                     /gene="rpsQ"
                     /locus_tag="WGQ68_000351"
     CDS             5960..6223
                     /gene="rpsQ"
                     /locus_tag="WGQ68_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEZ0714225.1"
                     /translation="MSETKNVRTLQGKVVSDKMDKTVTVLVERKVKHPLYGKIIRLST
                     KIHAHDENNQYGIGDVVVISESRPLSKTKSWVVSELVEKARSI"
     gene            6445..6813
                     /gene="rplN"
                     /locus_tag="WGQ68_000352"
     CDS             6445..6813
                     /gene="rplN"
                     /locus_tag="WGQ68_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009124801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEZ0714226.1"
                     /translation="MIQMQTILDVADNSGARRVMCIKVLGGSKRRYASVGDIIKVAVK
                     DAAPRGRVKKGDVYNAVVVRTAKGVRRPDGALIKFDNNAAVLLNNKLEPLGTRIFGPV
                     TRELRTERFMKIVSLAPEVL"
     gene            6825..7148
                     /gene="rplX"
                     /locus_tag="WGQ68_000353"
     CDS             6825..7148
                     /gene="rplX"
                     /locus_tag="WGQ68_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEZ0714227.1"
                     /translation="MNKIIKGDRVVVIAGKDKGKQGQVVRVLGDKVVVEGVNVVKRHQ
                     KPNPMRGIEGGIITKEMPLDISNIAILNPETNKADRVGIKLIENEGKVKRVRFFKSNG
                     SIIGA"
     gene            7158..7697
                     /gene="rplE"
                     /locus_tag="WGQ68_000354"
     CDS             7158..7697
                     /gene="rplE"
                     /locus_tag="WGQ68_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEZ0714228.1"
                     /translation="MARLREFYKETVVPELVKQFGYKSVMEVPRIEKITLNMGVGEAV
                     ADKKVMEHAVSDLEKIAGQKPVVTVARKSIAGFKIRDNYPVGCKVTLRRDQMFEFLDR
                     LITIALPRVRDFRGVSGKSFDGRGNYNMGVREQIIFPEIEYDKIDALRGLNITITTTA
                     KTDEEAKALLSLFKFPFKG"
     gene            7700..8005
                     /gene="rpsN"
                     /locus_tag="WGQ68_000355"
     CDS             7700..8005
                     /gene="rpsN"
                     /locus_tag="WGQ68_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEZ0714229.1"
                     /translation="MAKKALINRDLKRQALAKKYAAKRAAIKAVINDSNATEEERFEA
                     RLRFQSIPRNAAPVRQRRRCALTGRPRGTFRKFGLGRIKIREIAMRGEIPGVVKASW"
     gene            8021..8413
                     /gene="rpsH"
                     /locus_tag="WGQ68_000356"
     CDS             8021..8413
                     /gene="rpsH"
                     /locus_tag="WGQ68_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEZ0714230.1"
                     /translation="MSMHDPISDMLTRIRNAQRANKAAVAMPSSKLKCAIAKVLKEEG
                     YIEDFAVSSDVKSILEIQLKYYAGRPVIEQIKRVSRPGLRIYKASSEIPSVMNGLGIA
                     IVSTSKGVMTDRKARSQGVGGELLCIVA"
     gene            8427..8960
                     /gene="rplF"
                     /locus_tag="WGQ68_000357"
     CDS             8427..8960
                     /gene="rplF"
                     /locus_tag="WGQ68_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEZ0714231.1"
                     /translation="MSRVAKNPVTVPAGVEVKFGTEALVIKGKNGELSFPLHSDVAIE
                     FNDGKLAFVANNSSKQANAMSGTARALVSNMVKGVSEGFEKRLQLIGVGYRAQAQGKI
                     LNLSLGFSHPIVYEMPEGVSVQTPSQTEIVLTGSDKQVVGQVAAEIRAFRAPEPYKGK
                     GVRYVGEVVVMKEAKKK"
     gene            8974..9327
                     /gene="rplR"
                     /locus_tag="WGQ68_000358"
     CDS             8974..9327
                     /gene="rplR"
                     /locus_tag="WGQ68_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEZ0714232.1"
                     /translation="MDKHTTRLRRARKTRARIADLKMVRLCVFRSNNHIYAQVISAEG
                     DKVLAQASTLEAEVRGSLKSGSNVEAAAIVGKRIAEKAKAAGVEKVAFDRSGFQYHGR
                     VKALAEAARENGLSF"
     gene            9346..9864
                     /gene="rpsE"
                     /locus_tag="WGQ68_000359"
     CDS             9346..9864
                     /gene="rpsE"
                     /locus_tag="WGQ68_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEZ0714233.1"
                     /translation="MAKHEIEERGDGLIEKMVAVNRVTKVVKGGRIMAFSALTVVGDG
                     DGRIGMGKGKSKEVPVAVQKAMDQARRSMIKVPLKNGTIHHEVIGRHGATKVFMQPAK
                     EGSGVKAGGPMRLVFDAMGIHNISAKVHGSTNPYNIVRATLDGLSKLHTPADIAAKRG
                     LTVEDILGVNHG"
     gene            9857..10042
                     /gene="rpmD"
                     /locus_tag="WGQ68_000360"
     CDS             9857..10042
                     /gene="rpmD"
                     /locus_tag="WGQ68_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEZ0714234.1"
                     /translation="MAEQKKIRVTLVKSLIGTIESHRACARGLGLRRREHTVEVLDTP
                     ENRGMINKISYLLKVES"
     gene            10044..10478
                     /gene="rplO"
                     /locus_tag="WGQ68_000361"
     CDS             10044..10478
                     /gene="rplO"
                     /locus_tag="WGQ68_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEZ0714235.1"
                     /translation="MFLNTIQPAVGATHAGRRVGRGIGSGLGKTGGRGHKGQKSRSGG
                     FHKVGFEGGQMPLQRRLPKRGFKSLTASANAQLRLSELESIAVNEIDILVLKQAGLIA
                     STVSNVKVIASGEISKAVALKGIKVTKGARAAIEAVGGKIEM"
     gene            10490..11800
                     /gene="secY"
                     /locus_tag="WGQ68_000362"
     CDS             10490..11800
                     /gene="secY"
                     /locus_tag="WGQ68_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEZ0714236.1"
                     /translation="MANQQTSSGSSKFGDLKKRLLFLFGALIVFRIGAHIPVPGVDAV
                     ALAKLYESAGNGILGILNMFSGGSLERFSIFAIGIMPYISASIIVQLASEILPSLKAL
                     KKEGEAGRKVITKYTRYGTVLLAILQSLGVASFVFQQGIVVTSSFEFHVSTVVSLVTG
                     TMFLMWLGEQITERGIGNGISLIITAGIASGIPSGIAKLVTLTNQGSMSMLTALFIVF
                     GALLLIYLVVYFESAQRKIPIHYAKRQFNGRAGSQNTHMPFKLNMAGVIPPIFASSII
                     LFPSTLLGWFGSADTNSVLHKIAGLLQHGQLLYMALFAATVIFFCYFYTALVFSPKEM
                     AENLKKSGAFVPGIRPGEQTSRYLEKVVLRLTLFGALYITTICLIPEFLTTVLNVPFY
                     LGGTSLLILVVVTMDFSTQINSYRLTQQYDKLMTRLEMKSFSRK"
     gene            11805..12023
                     /gene="infA"
                     /locus_tag="WGQ68_000363"
     CDS             11805..12023
                     /gene="infA"
                     /locus_tag="WGQ68_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEZ0714237.1"
                     /translation="MAKEDTIQMQGEILETLPNATFKVKLENDHIVLGHISGKMRMHY
                     IRISPGDKVTVELTPYDLTRARIVFRAR"
     gene            12044..12157
                     /gene="rpmJ"
                     /locus_tag="WGQ68_000364"
     CDS             12044..12157
                     /gene="rpmJ"
                     /locus_tag="WGQ68_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006232360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEZ0714238.1"
                     /translation="MRVQPSVKKICRNCKIIRRNRVVRVICTDLRHKQRQG"
     gene            12223..12585
                     /gene="rpsM"
                     /locus_tag="WGQ68_000365"
     CDS             12223..12585
                     /gene="rpsM"
                     /locus_tag="WGQ68_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEZ0714239.1"
                     /translation="MARIAGVNIPNNAHIVIGLQAIYGIGATRAKLICEAANIAPDTK
                     AKDLDETQLDALRDQVAKYEVEGDLRREVTMSIKRLMDMGCYRGFRHRRGLPCRGQRT
                     RTNARTRKGPRKAIAGKK"
     gene            12605..13000
                     /gene="rpsK"
                     /locus_tag="WGQ68_000366"
     CDS             12605..13000
                     /gene="rpsK"
                     /locus_tag="WGQ68_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEZ0714240.1"
                     /translation="MAKANTASRVRKKVRKTVSEGIVHVHASFNNTIITITDRQGNAL
                     SWATSGGAGFKGSRKSTPFAAQVAAEAAGKVAQEYGVKNLEVRIKGPGPGRESSVRAL
                     NALGFKITSITDVTPLPHNGCRPPKKRRI"
     gene            13020..13640
                     /gene="rpsD"
                     /locus_tag="WGQ68_000367"
     CDS             13020..13640
                     /gene="rpsD"
                     /locus_tag="WGQ68_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004282443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEZ0714241.1"
                     /translation="MARYIGPKCKLARREGTDLFLKSARRSLDSKCKIDSAPGQHGAK
                     KPRLSDYGLQLREKQKIRRIYGVLERQFRRYFAEADRRKGSTGELLLQLLESRLDNVV
                     YRMGFGSTRAEARQLVSHKAIVVNGQVVNIPSFQVKAGDVVSVREKAKKQVRIQEALG
                     LATQIGLPGWVSVDADKLEGVFKNMPDRSELTGDINEQLVVEFYSK"
     gene            13666..14652
                     /gene="rpoA"
                     /locus_tag="WGQ68_000368"
     CDS             13666..14652
                     /gene="rpoA"
                     /locus_tag="WGQ68_000368"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEZ0714242.1"
                     /translation="MQNSTTEFLKPRQIDVNTFSATRAKVSMQPFERGFGHTLGNALR
                     RILLSSMNGFAPTEVAIAGVLHEYSTVDGIQEDVVDILLNIKGIVFKLHGRSQVQLVL
                     KKSGSGVVSAGDIELPHDVEILNPGHVICHLADNGQIEMEIKVEQGRGYQSVSGRQVV
                     RDENRQIGAIQLDASFSPISRVSFEVEPARVEQRTDLDKLVLDIETDGSIDPEEAVRS
                     AARILIDQMSIFADLQGTPVEEVEEKAPPIDPVLLRPVDDLELTVRSANCLKAEDIYY
                     IGDLIQRTETELLKTPNLGRKSLNEIKEVLASKGLTLGSKLEAWPPVGLEKP"
     gene            14676..15044
                     /gene="rplQ"
                     /locus_tag="WGQ68_000369"
     CDS             14676..15044
                     /gene="rplQ"
                     /locus_tag="WGQ68_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEZ0714243.1"
                     /translation="MRHRNGNRKLNRTSSHRAAMLRNMANSLLTHEAIVTTLPKAKEL
                     RRVVEPLITLGKKPSLANRRLAFDRTRDRDVVVKLFGDLGPRFTARNGGYVRVLKYGF
                     RKGDNAPLALVELVDKPAAE"
     gene            15191..15904
                     /gene="minC"
                     /locus_tag="WGQ68_000370"
     CDS             15191..15904
                     /gene="minC"
                     /locus_tag="WGQ68_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HEZ0714244.1"
                     /translation="MMVYIMNAFDIKSTKMDVLSISLHTSDLFDLEDVLVKLGKKFQE
                     SGVVPFVLDVQEFDYPESLDLAALVSLFSRHGMQILGLKHSNERWAAAAMKYHLLFCL
                     SHSENVKELGQVEVQNTEDGQKARKTVLITSPVRTGQQVYAEDGDLIVTGAVSQGAEL
                     IADGNIHIYAPMRGRALAGAKGDTSARIFIHSMQAELVSVAGIYRNFEQDLPNHLHKQ
                     PVQILLQDNRLVISAIGSE"
     gene            15933..16748
                     /gene="minD"
                     /locus_tag="WGQ68_000371"
     CDS             15933..16748
                     /gene="minD"
                     /locus_tag="WGQ68_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HEZ0714245.1"
                     /translation="MAKIIVVTSGKGGVGKTTTSASIATGLALRGYKTAVIDFDVGLR
                     NLDLIMGCERRVVYDLINVIQGEATLNQALIKDKNCENLFILPASQTRDKDALTREGV
                     EKVMQELSGKKMGFEYIICDSPAGIEQGALMALYFADEAIVTTNPEVSSVRDSDRILG
                     ILQSKSHKAEQGGSVKEHLLITRYSPERVAKGEMLSVQDICDILHIPLLGVIPESQNV
                     LQASNSGEPVIHQDSVAASEAYKDVIARLLGENREMRFLEAEKKSFFKRLFGG"
     gene            16752..17015
                     /gene="minE"
                     /locus_tag="WGQ68_000372"
     CDS             16752..17015
                     /gene="minE"
                     /locus_tag="WGQ68_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HEZ0714246.1"
                     /translation="MSLIEFLFGRKQKTATVARDRLQIIIAQERAQEGQAPDYLPTLR
                     KELMEVLSKYVNVSLDNIRISQEKQDGMDVLELNITLPEQKKV"
     gene            17019..17939
                     /gene="oxyR"
                     /locus_tag="WGQ68_000373"
     CDS             17019..17939
                     /gene="oxyR"
                     /locus_tag="WGQ68_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator OxyR"
                     /protein_id="HEZ0714247.1"
                     /translation="MTLTELRYIVAVAQERHFGRAARRCFVSQPTLSIAIKKLEEELA
                     VSLFDRSSNDIITTEAGERIVAQARKVLEEAELIRHLANEEQNELEGAFKLGLIFTVA
                     PYLLPKLIVSLRRTAPKMPLMLEENYTHTLTESLKRGDVDAIIVAEPFQEPGIVTEPL
                     YDEPFFVIVPKGHSFEELDAVSPRMLGEEQVLLLTEGNCMRDQVLSSCSELAAKQRIQ
                     GLTNTLQGSSINTIRHMVASGLAISVLPATALTENDHMLFSIIPFEGTPPSRRVVLAY
                     RRNFVRPKALSAMKAAIMQSQLHGVSFIRD"
     gene            complement(17936..18127)
                     /locus_tag="WGQ68_000374"
     CDS             complement(17936..18127)
                     /locus_tag="WGQ68_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714248.1"
                     /translation="MLIHYTWHCIGTDENIHSDAKVEYLQDGSFRGDAILKIDDDGNI
                     LAYRVVGSGKWRFENNACA"
     gene            complement(18269..21106)
                     /locus_tag="WGQ68_000375"
     CDS             complement(18269..21106)
                     /locus_tag="WGQ68_000375"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEZ0714249.1"
                     /translation="MLDKYNPAEIESKHYQNWEEQGYFQPDMDLTKPSFSIQLPPPNV
                     TGTLHMGHAFNQTIMDGLTRYYRMKGCNTAWIPGTDHAGIATQIVVERQLAAQNVSRH
                     DLGREKFLEKVWEWKEVSGGTITQQMRRVGCSADWTREYFTMDDVRAETVTEVFVRLY
                     EQGLIYRGKRLVNWDPVLGTAVSDLEVESVEEQGSMWHIRYPLADNPAEAVIVATTRP
                     ETLLGDVAVAVNPEDERYTHLIGKELILPLTGRTIPVIADEYVEKDFGTGCVKITPAH
                     DFNDYEVGKRHDTRLINVFDLEAKVLANAEVFNFKGEAQQGFALPEKYAGLDRFAARK
                     QMVADLQEQGFLVEIKAHTLMTPKGDRTGSVIEPMLTSQWFVAMSATPNGGEPDSEFK
                     GLSLADKAKKAVDSGAVRFIPENWVNTYNQWMNNIQDWCISRQLWWGHQIPAWYDEAG
                     NVYVARNQVEAEKQAGKTGLTREEDVLDTWFSSALVPFSTLGWPSETDELKAFLPSNV
                     LVTGYEIIFFWVARMIMMTTHFTGKVPFKDVYIHGIVRDHEGKKMSKSEGNVIDPVDL
                     IDGIGLDKLLVKRTTGLRKPETAPKVEEATKKLFPEGIPSMGADALRFTMASYASLGR
                     SVNFDFKRAEGYRNFCNKIWNATNFVLMNTENQDCGYGATATEPRGYSFPDMWIVDRL
                     NQTIEQVTQAYETYRFDLAAETLYSFVWNDYCDWYLELAKVQLQTGCASRQRATRHTL
                     LRVLEAALRLLHPIIPFITEELWQTVAPMCDAKTADSIMLARFPEADGGEIVQTAFGQ
                     MTVLQDLIGAVRNLRGEMGIQPNVKAPLFVESTDDLADYLKYLPMMTRLTEAQQVAAL
                     PESEDAPVAVCNGARLMLKVEIDKAAETARLSKEAEKLQKALDKLNAKLSKPGYTEKA
                     PAHLVEKDKADLAELEDKMAKVQNQLAKLKD"
     gene            complement(21203..22012)
                     /gene="zupT"
                     /locus_tag="WGQ68_000376"
     CDS             complement(21203..22012)
                     /gene="zupT"
                     /locus_tag="WGQ68_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HEZ0714250.1"
                     /translation="MPDFSMSNLAVAFSITLAAGLFTVLGSGLVMFSKTPNPRVLSFG
                     LAFAGGAMVYVSLTEIFSKSSEAFAEIYDKDHAFAAATMAFLAGMGGIALIDRLVPNP
                     HETLDAQDPSFQESKRRHIARVGMMAAFAITAHNFPEGLATFFATLENPAVGMPLALA
                     IAIHNIPEGISIAAPVYFATRSRKKTVWACLLSGLAEPLGAALGYLVLQPFLSPAVFG
                     SVFGVIAGVMVFLALDELLPAAKRYSDGHETVYGLTMGMAVIAVSLVLFHF"
     gene            complement(22193..23449)
                     /locus_tag="WGQ68_000377"
     CDS             complement(22193..23449)
                     /locus_tag="WGQ68_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HEZ0714251.1"
                     /translation="MKVLVLGAGVAGVSSAWYLAEAGHEVTVIDRAEGVAMETSFANA
                     GQLSYGYTTPWAAPGIPTKALKWLFKSHPPLLFRPDGSLYQIEWLWQMLQNCTAAHYQ
                     TNKERMVRISEYSREMFRRFEAQTGMNFEERKKGTLQIFRQTKEVEAAEQDIAVLERY
                     GVPYRRLKPEECAEFEPALARVTAKIASGLHLPADATGDCRLFTENLYKLCQEKGVRF
                     HFNQTISRIDHNGLRIKTVETETGRFEADAVVCALGCFSRTVLAQLDLNLPIYPVKGY
                     SLTLPVTNSDGAPVSTVLDESYKVAITRFDNRIRVGGMAELSGYEIKLPEKRRETLAL
                     VVNDLFPEGGDLSQASFWSGLRPMTPDSTPLIGRTRFDNLFLNTGHGTLGWTMSLGSA
                     KLTADIVSGKDTEIRSDDLSLSRYQA"
     gene            complement(23528..24919)
                     /locus_tag="WGQ68_000378"
     CDS             complement(23528..24919)
                     /locus_tag="WGQ68_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0714252.1"
                     /translation="MQLFLDNLKAFFETISGWVWGSVMLVLLVGTGILLTVLLKGLQF
                     TMLGYALKQAFVPSKKHEDGEDHEGDISHFAALMTALSATIGTGNIAGVATAVVTGGP
                     GAVFWMWMTAIFGMATKYGEGVLAVKYRVTNSKGEMSGGPMYYIEKGLGKNWKWMAVA
                     FALFGTFASFGIGSSVQSNSVAQAVQTSFGIEPAYTGITLTVLTAVVVLGGIKGIAKA
                     ASFIVPAMAVFYVLGGLSIIAINSDALMPAVKLIFSDAFSAQAVAGGAIGTVIRYGVA
                     RGVFSNEAGMGSAPIAAAAAKTDHPVRQALVSMTGTFLDTIVVCSITGIVLVMGLLGA
                     GGEFVKPEVSGAALTTVTFQKMLPGIGGWIVTIGLIFFAYSTILGWCYYGEKCAVYVF
                     GEKFAGLYRVGYVSSVMLGTVLSLDLVWLASDTFNGLMALPNLIALLLMAKVIVNETR
                     DFKQKITNGELPH"
     gene            complement(25276..26052)
                     /gene="lpxA"
                     /locus_tag="WGQ68_000379"
     CDS             complement(25276..26052)
                     /gene="lpxA"
                     /locus_tag="WGQ68_000379"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEZ0714253.1"
                     /translation="MTLIHPTAVIDPKAELDSSVKVGAYTVIGPNVQIGANTEIGPHA
                     VINGHTSIGENNRIFQFASLGEIPQDKKYRDEPTRLIIGNGNTIREFTTFNLGTVTGI
                     GETRIGDDNWIMAYCHLAHDCVVGNHTIFANNASLAGHVTIGDYVVLGGYTLVFQFCR
                     IGDYAMTAFAAGVHKDVPPYFMASGYRAEPAGLNSEGMRRNGFTAEQISAVKDVYKTL
                     YHRGIPFEEAKADILRRAETQAELAVFRDFFAQSARGIIR"
     gene            complement(26139..26588)
                     /gene="fabZ"
                     /locus_tag="WGQ68_000380"
     CDS             complement(26139..26588)
                     /gene="fabZ"
                     /locus_tag="WGQ68_000380"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEZ0714254.1"
                     /translation="MDVQLPIEAKDIQKLIPHRYPFLQLDRITAFEPMKTLTAIKNVT
                     INEPQFQGHFPDLPVMPGVLIIEAMAQACGTLAILSEGGRKENEFFFFAGIDEARFKR
                     QVIPGDQLVFEVELLTSRRGIGKFNAVAKVDGQVAVEAVIMCAKRVV"
     gene            complement(26622..27668)
                     /gene="lpxD"
                     /locus_tag="WGQ68_000381"
     CDS             complement(26622..27668)
                     /gene="lpxD"
                     /locus_tag="WGQ68_000381"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEZ0714255.1"
                     /translation="MIPATYTLSQITAQLGGEWRGEDISVTAVRPLADAQAEHISFLA
                     NPKYKAEVHDSSAGAVIVSAKAADGFEGRNLIVADDPYLYFAKVARLFSPVVKARGGI
                     HPTAVVEPSATVPASCEIGANAYIGANTVLGEGCRILANAVVQHDCKLGDEVVLHPNA
                     VVYYGCTLGRRVEIHSGAVIGADGFGLAFAGDSWFKIPQTGAVTLGDDVEIGSNTNID
                     RGAMSDTTVGNGTKIDNQVQIGHNCKIGSHTVIAAKTGISGSVTIGSYCIIGGGVGTV
                     GHIEIADKTTIGGGTSVTHSITESGKHLAGIFPMSTHKEWARNAVYIHRLSEMNKRLK
                     TLEQQLSDAGQDSK"
     gene            complement(27701..28201)
                     /locus_tag="WGQ68_000382"
     CDS             complement(27701..28201)
                     /locus_tag="WGQ68_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEZ0714256.1"
                     /translation="MTRLTRAFAAALIGLCCTAGAHADTFQKIGFINTERIYLESKQA
                     RKIQKTLDSEFSARQDELQKLQREGLDLERQLAEGKLRNAKKAQAEEKWRGLVAAFRK
                     KQAQFEEDYNLRRNEEFASLQQNANRVIVKIAKQEGYDVILQNVIYVNTQYDVTDSVI
                     KEMNAR"
     gene            complement(28267..30660)
                     /gene="bamA"
                     /locus_tag="WGQ68_000383"
     CDS             complement(28267..30660)
                     /gene="bamA"
                     /locus_tag="WGQ68_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEZ0714257.1"
                     /translation="MKLKQIASALMVLGISPLAFADFTIQDIRIEGLQRTEPSTVFNY
                     LPVKIGDAYNDGRGGEIIKSLYATGFFDDVRVESADGQLLLTVIERPTIGSLNITGAK
                     MLQNDAIKKNLESFGLAQSQYFNQATLNQAVAGLKEEYLGRGKLNIQITPKVTKLARN
                     RVDIDITIDEGKSAKITDIEFEGNQVYSDRKLMRQMSLTEGGIWTWLTRSNQFNEQKF
                     AQDMEKVTDFYQNNGYFDFRILDTDIQTNEDKTKQTIKITVHEGERFRWGKVSIEGDT
                     NEVPKAELEKLLTMKPGKWYERQQMTAVLGEIQNRMGSAGYAYSEISVQPLPNAETKT
                     VDFVLHIEPGRKIYVNEIHITGNNKTRDEVVRRELRQMESAPYDTSKLQRSKERVELL
                     GYFDNVQFDAVPLAGTPDKVDLNMSLTERSTGSLDLSAGWVQDTGLVMSAGVSQDNLF
                     GTGKSAALRASRSKTTLNGSLSFTDPYFTADGVSLGYDVYGKAFDPRKASTSIKQYKT
                     TTAGAGIRMSVPVTEYDRVNFGLVAEHLTVNTYNKAPKHYADFIKKYGKTDGTDGSFK
                     GWLYKGTVGWGRNKTDSALWPTRGYLTGVNAEIALPGSKLQYYSATHNQTWFFPLSKT
                     FTLMLGGEIGYANGYGKTKEIPFFENFYGGGLGSVRGYESGTLGPKVYDEYGEKISYG
                     GNKKANVSAELLFPMPGAKDARTVRLSLFADAGSVWDGKTYDDNSSSATGGRVQNIYG
                     AGNTHKSTFTNELRYSAGGAVTWLSPLGPMKFSYAYPLKKKPEDEIQRFQFQLGTTF"
     gene            complement(30715..32055)
                     /gene="rseP"
                     /locus_tag="WGQ68_000384"
     CDS             complement(30715..32055)
                     /gene="rseP"
                     /locus_tag="WGQ68_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEZ0714258.1"
                     /translation="MHTLLAFIFAILILVSLHEFGHYIVARLCGVKVVRFSVGFGKPF
                     FTRKRGDTEWCLAPIPLGGYVKMVDTREGEVSEADLPYAFDKQHPAKRIAIVAAGPLT
                     NLALAVLLYGLSFSFGVTELRPYVGTVEPDTIAARAGFQSGDKIQSVNGTPVADWGSA
                     QTEIVLNLEAGKVAVGVQTASGAQTVRTIDAASTPEAGKIAKNQGYIGLMPFKITTVA
                     GGVEKGSPAEKAGLKPGDRLTAADGKPITSWQEWANLTRQSPGRKIALTYERAGQTHT
                     ADIRPDTVEQSDHTLIGRVGLRPQPDRAWDAQIRRSYRPSVVRAFGMGWEKTVSHSWT
                     TLKFFGKLISGNASVSHISGPLTIADIAGQSAELGLQSYLEFLALVSISLGVLNLLPV
                     PVLDGGHLVFYTAEWIRGKPLGERVQNIGLRFGLALMMLMMAIAFFNDITRLLG"
     gene            complement(32090..33325)
                     /locus_tag="WGQ68_000385"
     CDS             complement(32090..33325)
                     /locus_tag="WGQ68_000385"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEZ0714259.1"
                     /translation="MPSENASDGIRYKVIPIMTPQVLTILGSTGSIGESTLDVVSLHP
                     EKFRVFALAGHKQVEKLAVQCRTFRPEYAVVADAEHAARLEALLKGDGTATQVLHGAQ
                     ALVDVASADEVSGVMCAIVGAVGLPSALAAAQKGKTIYLANKETLVVSGALFMETARA
                     NGAAVLPVDSEHNAVFQVLPRDYAGRLNEHGIASIILTASGGPFLTADLNTFDSITPD
                     QAVKHPNWRMGRKISVDSATMMNKGLELIEAHWLFNCPPDKLEVVIHPQSVIHSMVRY
                     RDGSVLAQLGNPDMRTPIAYCLGLPERIDSGVGDLDFDALSALTFQKPDFDRFPCLKL
                     AYEAMNAGGAAPCVLNAANEAAVAAFLDGQIKFTDIAKTVAHCLAQDFSDGIGDIGGL
                     LAQDARTRAQARAFIGTLR"
     gene            complement(33330..34127)
                     /locus_tag="WGQ68_000386"
     CDS             complement(33330..34127)
                     /locus_tag="WGQ68_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEZ0714260.1"
                     /translation="MLKQRVITAMWLLPLMLGMLFYAPQWLWAAFCGLIALIALWEYA
                     RMGGLCKIKTNHYLAATLVFGVVAYAGGWMLPNLVWYVVLAFWLAVMPLWLRFKWRLN
                     GGWQVYAVGWLLVMPFWFALVSLRPHPDDALPLLAVMGLVWVADICAYFSGKAFGKHK
                     IAPAISPGKSWEGAIGGAVCVAVYMTAVRSAGWLAFDTGWFDTVLIGLVLTVVSVCGD
                     LLESWLKRAAGIKDSSNLLSGHGGVFDRTDSLIAVISVYAAMMSVLN"
     gene            complement(34130..34876)
                     /locus_tag="WGQ68_000387"
     CDS             complement(34130..34876)
                     /locus_tag="WGQ68_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEZ0714261.1"
                     /translation="MKSSTQAVLEHTAIPKHIAVIMDGNGRWAKKRFLPRIMGHKRGL
                     DALENMVKHCAKLGVQYLTVFAFSTENWRRPEDEVSFLMGLFLQALQKQVRRLHENNM
                     RLKILGSRERFSRQILKGIEEAEALTANNTGLTLSIAADYGGRWDILQAANKLIAKGV
                     SEITEDTLAKHLMLGDAPEPDLFIRTGGETRISNFLLWQMAYAELYFTDILWPDFDGK
                     ALDDAVASFQKRERRFGRTSEQLPIEQQRN"
     gene            complement(34932..35489)
                     /gene="frr"
                     /locus_tag="WGQ68_000388"
     CDS             complement(34932..35489)
                     /gene="frr"
                     /locus_tag="WGQ68_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEZ0714262.1"
                     /translation="MINDIQKTAEGKMQRSVEVLKENLAKVRTGRAHTGLLDQVEVEY
                     WGSMVPVSQVANVTLLDARTIGVKPFEGNMAVKVEKAIRDSNLGLNPAAVGDLIRVPM
                     PMLTEERRKDLIKVVRGEAEEGRVSIRNVRRDANDHIKKLLKDKEISEDEARRGEEAV
                     QKLTDKYITEADKLLTAKEEDLMAI"
     gene            complement(35669..36796)
                     /locus_tag="WGQ68_000389"
     CDS             complement(35669..36796)
                     /locus_tag="WGQ68_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEZ0714263.1"
                     /translation="MNTSQRNRLVSRWLNSYERYRYRRLIHAIRLGGAVLFATASARL
                     LHLQHGEWIGMTVFVVLGMLQFQGAIYSKAVERMLGTVIGLGAGLGVLWLNQHYFHGN
                     LLFYLTVGTASALAGWAAVGKNGYVPMLAGLTMCMLIGDNGSEWFDSGLMRAMNVLIG
                     AAIAIAAAKLLPLKSTLMWRFMLADNLTDCSKMIAEISNGRRMTRERLEENMAKMRQI
                     NARMVKSRSHLAATSGESRISPAMMEAMQHAHRKIVNTTELLLTTAAKLQSPKLNGSE
                     IRLLDRHFTLLQTDLQQTVALINGRHARRIRIDTAINPELEALAEHLHYQWQGFLWLS
                     TNMRQEISALVILLQRTRRKWLDAHERQHLRQSLLETREHG"
     gene            complement(36872..36947)
                     /locus_tag="WGQ68_000390"
     tRNA            complement(36872..36947)
                     /locus_tag="WGQ68_000390"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36912..36914),aa:Thr,seq:cgt)
BASE COUNT         8760 a         8437 c        10069 g         9751 t
ORIGIN      
        1 tggctgccgc attggacagc tacatcccga ctcccgagcg tgccgtggac aaaccgttcc
       61 tgctgcctat cgaagacgtg ttctccattt ccggccgcgg tacagtagta accggccgtg
      121 tagagcgcgg tatcatccac gttggtgacg agatcgaaat cgtcggtctg aaagaaaccc
      181 aaaaaaccac ctgtaccggc gttgaaatgt tccgcaaact gctggacgaa ggtcaggcgg
      241 gcgacaacgt aggcgtattg ctgcgcggta ccaaacgtga agacgtagag cgtggtcagg
      301 tattggccaa accgggtact atcactcctc acaccaagtt caaagcagaa gtgtacgtac
      361 tgagcaaaga agagggcggc cgccataccc cgttcttcgc caactaccgt ccccaattct
      421 acttccgtac caccgacgta accggcgcgg ttactttgga agaaggtgtg gaaatggtaa
      481 tgccgggtga gaacgtaacc attaccgtag aactgattgc gcctatcgct atggaagaag
      541 gcctgcgctt tgcgattcgc gaaggcggcc gtaccgtggg tgccggcgtg gtttcttctg
      601 ttatcgctta attgaaggat attgataaat ggcaaaccaa aaaatccgta tccgcctgaa
      661 agcttatgat tacgccctga ttgaccgttc tgcacaagaa atcgttgaaa ctgcaaaacg
      721 taccggtgcc gttgtaaaag gcccgattcc tttgccgacc aaaatcgaac gtttcaacat
      781 tctgcgttct ccgcacgtga acaaaacttc ccgtgaacaa ttggaaatcc gcacccactt
      841 gcgcctgatg gacatcgtgg attggaccga taaaactacc gatgcgctga tgaagctgga
      901 tttgccggcc ggtgttgatg tagaaatcaa agtccaataa ttcggactat tgaaaatccc
      961 caagcaatca atgcttgggg attttttatg ttatgccgag acctttgcaa aattccccaa
     1021 aatcccctaa attcccacca agacatttag gagattttcc atgagcacct tctttcaaca
     1081 aaccgcacaa gccatgattg ctaaacacat cgaccgcttc ccgctattga agttggaccg
     1141 ggtgattgat tggcagctga tcgaacaata cctgaaccgt caaaaaaccc gttaccttag
     1201 agaccaccgc ggccgtcccg cctatcccct gctatccatg ttcaaagccg tcctgctcgg
     1261 acaatggcac agcctctccg atcccgaact cgagcacagc ctcatcaccc gcatcgattt
     1321 caacctgttt tgccgctttg acgaactgag catccccgat tacagtcatc aaccatattc
     1381 cggtttgtcg gagaaagatg catacgctgt gatgaccgga taccgaccct ttaaaagagt
     1441 ccgaccctat gccgtctgaa aattcaaaac gcttcagacg gcatattgaa gatatttctg
     1501 atatttctgt tgatatttct ttaacttgtc agatataatg ccgagcttgg tacatttgtg
     1561 ccaagtttaa ctttgtctga aagacaggcc aatcgtagcc tgtcccttta ctttaaaagg
     1621 aaaataatca tgactttagg tctggttgga cgcaaagttg gtatgacccg cgtgttcgac
     1681 gaacagggtg tttctgttcc ggtaaccgtt ttggatatgt ctgccaaccg cgttacacaa
     1741 gtaaaatcca aagatactga cggctatact gccgttcaag ttacctttgg tcagaaaaaa
     1801 gccaatcgtg tcaacaaagc cgaagccggg cactttgcaa aagcaggtgt tgaagccggt
     1861 cgcggtttga ttgagtttgc tttgactgaa gaaaaactgg ctgaattgaa agctggtgac
     1921 gaaatcaccg tttctatgtt tgaagtcggt caactggtcg atgtaaccgg tatctctaaa
     1981 ggtaaaggtt tctccggcac gattaaacgt cataacttcg gtgcccaacg tacttcccac
     2041 ggtaactccc gttctcaccg cgttccaggt tctatcggta tggcgcaaga cccgggtcgc
     2101 gtgttccccg gtaaacgcat ggccggccaa tacggcaaca ccaaagcaac tgttcaaaaa
     2161 ttggaagttg tccgtgttga tgcagaacgc caactgctgt tggttaaggg tgctgttccg
     2221 ggtgcggtca acagcgatgt tgtagttcgt cccagcgtga aagtaggtgc gtaatggaat
     2281 tgaaagtaat tgacgctaaa ggacaagttt caggcagcct gtctgtttct gatgctttgt
     2341 tcgcccgcga atacaatgaa gcgttggttc atcagctggt aaatgcctac ttggcaaacg
     2401 cccgctctgg taaccgtgct caaaaaaccc gtgccgaagt aaaacactca accaaaaaac
     2461 catggcgtca aaaaggtacc ggccgcgccc gttccggtat gacttcatct ccgctgtggc
     2521 gtaaaggtgg tcgcgcgttc ccgaacaaac ccgacgaaaa cttcactcaa aaagtaaacc
     2581 gcaaaatgta ccgtgccggt atggcgacta ttctgtccca attgactcgt gacgagcgtt
     2641 tgtttgcgat tgaggcgttg actgccgaaa ctcctaaaac caaagttttt gctgaacaag
     2701 tgaaaaatct gggtctggag caagtgttgt ttgtaaccaa acagctcgac gagaatgttt
     2761 acttggcttc acgcaacttg ccaaacgtgt tggttttgga agctcaacaa gttgatcctt
     2821 acagcttgct gcgttacaaa aaagtaatca tcactaaaga tgcagttgca caattagagg
     2881 agcaatgggt atgaatcaac aacgtttgac tcaagtgatt ttggcaccta tcgtttctga
     2941 aaaaagcaac gtattggctg aaaaacgcaa ccaaatgacg tttaaagtct tggcaagtgc
     3001 aaccaaacct gaaatcaaag cggctgtcga gctgctgttt ggtgttcaag ttgcttctgt
     3061 aactaccgtt acaactaaag gcaaaactaa gcgttttggt cgtactttgg gccgccgccg
     3121 cgatgttaaa aaagcttatg taagcttggc tgccggtcaa gagttggatt tggaagccgc
     3181 tgctgcagct gcagataagg aataaacaaa atggcaatcg ttaaaatgaa gccgacctct
     3241 gcaggccgtc gcggcatggt tcgcgtggta acagaaggtt tgcacaaagg tgcgccttat
     3301 gcacctttgc tcgaaaagaa aaattctact gctggtcgta acagtaatgg tcatatcacc
     3361 acccgtcaca aaggcggcgg tcataaacac cattaccgtg ttgtagactt taaacgtaac
     3421 aaagacggca ttcctgccaa agtagagcgt atcgaatacg atcctaaccg tactgccttc
     3481 atcgcactgt tgtgctatgc agacggcgag cgtcgttaca tcattgctcc tcgcggtatt
     3541 caagccggtg ccgtattggt ttccggtgct gaagctgcca tcaaagtagg caacaccctg
     3601 ccgattcgca acattcccgt tggtacgact atccactgta tcgaaatgaa acccggtaaa
     3661 ggtgctcaaa tcgcacgttc tgccggtgct tctgcggtat tgctggctaa agaaggcgcg
     3721 tacgctcaag tccgcctgcg ctctggcgaa gtccgtaaaa tcaacgtaaa ttgccgtgcg
     3781 accatcggtg aagttggtaa cgaagagcaa agcctgaaaa aaatcggtaa agccggtgcc
     3841 aaccgttggc gcggtattcg tccgaccgta cgcggtgttg tcatgaatcc tgtcgatcac
     3901 ccgcatggtg gtggtgaagg ccgtacgggc gaggcccgcg aaccggtcag cccatggggt
     3961 actcctgcta aaggctaccg cactcgtaat aacaaacgca cggataacat gattgttcgt
     4021 cgccgttact caaataaagg ttaatttagt atggctcgtt cattgaaaaa aggcccatat
     4081 gtagacctgc atttgctgaa aaaagtagat gctgctcgcg caagcaacga caaacgcccg
     4141 attaaaacct ggtctcgtcg ttctaccatt ctgcctgatt ttatcggtct gaccattgct
     4201 gtgcacaacg gccgcaccca tgtgcctgtg tttatcagcg acaatatggt tggtcataaa
     4261 ttaggcgaat tctcattgac ccgtaccttt aaaggccact tggccgataa aaaggctaaa
     4321 aagaaataag gtgaatcatg agagtaaatg cacaacataa aaatgcccgt atctctgctc
     4381 aaaaggctcg tttggtagct gatttgattc gtggtaaaga cgttgcccaa gctttgaata
     4441 ttttggcttt cagtcctaaa aaaggtgccg agctgattaa aaaagtattg gagtcagcta
     4501 ttgctaatgc cgagcacaat aacggtgcgg acattgatga actgaaagtg gtaactatct
     4561 ttgttgacaa aggcccaagc ttgaaacgtt ttcaagctcg cgccaaaggt cgcggtaacc
     4621 gcatcgaaaa acaaacttgt catatcaatg tgacagtggg taactaagga aaagctatgg
     4681 gacaaaagat taaccctaca ggctttcgcc tggcggtaac taaagactgg gcttcaaaat
     4741 ggtttgctaa aagcaccgac ttttctactg ttttgaagca ggatatcgat gttcgcaatt
     4801 atttgcgtca aaaattggcc aatgcttcgg ttggtcgagt ggttattgaa cgccctgcaa
     4861 aatctgcacg cattaccatt cactccgctc gtccgggtgt ggttatcggt aaaaaaggtg
     4921 aggatatcga ggttttgaaa cgtgacttgc aagtcttgat gggtgtacct gttcatgtaa
     4981 atattgaaga gatccgccgt cctgagttgg atgctcaaat tattgctgac ggtattgccc
     5041 agcagttgga aaagcgcgtt caattccgtc gtgctatgaa acgagcaatg caaaatgcaa
     5101 tgcgttctgg tgctaaaggc attaagatta tgacttcagg ccgtctgaat ggtgcggata
     5161 ttgcccgtag cgaatggtat cgtgaaggtc gcgtgccact gcatacttta cgtgcaaatg
     5221 tagattatgc aaccagcgaa gcgcacacca catatggtgt attgggtctg aaagtttggg
     5281 tttatacgga aggcaatatt aaatcttcca aacctgaaca tgagagtaaa caaagaaagg
     5341 caggtagacg taatgctgca gccaactaga ctgaaatacc gtaagcaaca aaagggtcgc
     5401 aataccggca tcgctactcg cggtaataag gtaagtttcg gtgagttcgg cttgaaagcc
     5461 gtaggtcgtg gtcgtttgac tgcccgtcaa atcgaagctg ctcgtcgtgc aatgacccgt
     5521 catatcaaac gtggtggtcg tatttggatt cgtgtattcc ctgataaacc gattactgaa
     5581 aagcctattc aagttcgtat gggtggcggt aaaggtaacg tggaatatta cattgccgaa
     5641 attaaaccag gtaaagtgtt gtatgaaatg gatggcgttc cagaggaact ggctcgtgaa
     5701 gcattcgagt tggctgctgc caaattgcct attcctacaa cctttgtagt aagacaggtg
     5761 ggtcaataat gaaagcaaat gaattgaaag acaaatccgt tgagcagttg aatgcagatt
     5821 tgttggactt gttgaaagct cagtttggct tacgtatgca aaacgctacc ggtcaattag
     5881 gcaaaccaag tgaattgaaa cgtgtacgtc gcgatattgc tcgtattaaa accgttttaa
     5941 ctgaaaaagg tgctaagtaa tgagcgaaac taaaaatgtt cgtactttgc aaggcaaagt
     6001 agtaagcgac aaaatggata aaaccgtaac agtattggtt gagcgtaaag taaaacatcc
     6061 gctttatggt aagattattc ggttatctac taaaatccat gcccatgatg aaaataatca
     6121 atatggaatt ggtgatgtgg ttgttatatc ggaatcccgt ccattgtcaa aaactaaatc
     6181 ttgggttgtc agtgagctgg ttgagaaagc acgttctatt taagaattaa agcaacgtgc
     6241 ttggaatggg aaacgaagta ttgcagcaaa tttaatttgc gtgtaaactt cgtttcctgt
     6301 ctttcagttt cttctggaag tttcttccct ttcggggtcc aagactggtt tacttgaacc
     6361 gcaaggtttc atttaataag cagcggcttt gctgtaagtt atctgaaagt ggtaaattaa
     6421 gttggttaat ttaaaggtaa taacatgatt caaatgcaga ccatcttaga tgtggctgat
     6481 aactctggtg cgcgtcgcgt aatgtgtatc aaggtattgg gcggatctaa gcgtcgctac
     6541 gcttctgttg gcgatattat taaagtggca gttaaagatg cggctccgcg tggccgtgtc
     6601 aaaaaaggcg atgtatataa tgcggtagtt gttcgtactg ctaagggtgt acgtcgtcct
     6661 gatggtgcgt taattaaatt cgataacaat gccgccgtgt tactgaataa taaacttgaa
     6721 cctttgggta ctcgtatctt tggtccggta acccgtgaat tgcgtactga gcgatttatg
     6781 aaaatcgttt cattggcacc tgaagtatta taaggaatgg cacgatgaat aaaatcatta
     6841 aaggcgatag ggttgtagta attgctggta aggataaagg taagcagggt caagtagttc
     6901 gagtgttggg tgataaagtt gttgttgagg gcgttaatgt tgtaaaacgc catcaaaaac
     6961 ctaatccaat gcgtggcatt gagggcggta ttattactaa agaaatgcct ttggatattt
     7021 ctaatatcgc aatcctgaat ccggaaacta ataaagcgga ccgtgttggt attaagctga
     7081 ttgaaaatga aggcaaagtt aaacgcgttc gtttcttcaa atcaaatggc tctatcattg
     7141 gggcataagg agataacatg gctcggttga gagagtttta taaagagaca gttgttcctg
     7201 aattggttaa acaatttggt tacaaatcag taatggaagt cccgcgtatt gaaaaaatta
     7261 ccttgaatat gggtgtgggt gaggctgttg ctgataaaaa agttatggaa catgctgttt
     7321 ccgatttaga gaaaattgcc ggtcaaaaac cggttgttac tgttgcccgt aaatctatcg
     7381 caggttttaa aatccgtgat aactatccgg ttggttgcaa agtaacattg cgtcgtgatc
     7441 aaatgtttga attcttggat cgtttgatta ctattgcatt acctcgcgta cgtgacttcc
     7501 gtggtgtgag cggtaaatca tttgatggcc gtggcaatta caatatgggt gttcgtgagc
     7561 aaattatttt tccggaaatt gaatacgata aaattgatgc tttgcgtggt ttgaatatta
     7621 ctattactac tacagcaaaa accgatgagg aagcgaaagc tttattgtca ttgtttaaat
     7681 ttccgttcaa aggataatca tggctaagaa agcacttatt aatcgtgatc tgaaacgtca
     7741 agctttggct aaaaaatatg cggctaaacg cgcggcaatt aaagcggtaa tcaatgattc
     7801 gaatgcaact gaggaagagc gttttgaggc tcgtttgagg tttcaatcca ttcctcgtaa
     7861 tgcggcacct gtgcgtcaac gtcgtcgttg tgctttgaca ggtcgccctc gtggtacttt
     7921 ccgtaaattt ggtttgggtc gtattaaaat ccgtgaaatc gccatgcgtg gcgaaattcc
     7981 gggtgttgtt aaagccagct ggtaatagga gtaattaaga atgagtatgc atgatcctat
     8041 ttccgatatg ttgactcgta tccgcaatgc gcaacgtgct aataaagcag cggttgcaat
     8101 gccttcttca aaattaaagt gtgctattgc aaaggtattg aaagaagaag gatatattga
     8161 ggacttcgca gtttcatctg acgtaaagtc tatattggaa attcaattaa aatactatgc
     8221 aggtcgtcct gtaattgaac aaatcaagcg tgtatctcgc cctggtttgc gtatttataa
     8281 agcgtctagt gagattccaa gtgttatgaa tggcttgggt attgctattg ttagtacttc
     8341 taaaggtgta atgactgatc gtaaagcacg ttctcaaggt gttggtggtg agttgttatg
     8401 cattgtagcc tagtggagga aaagaaatgt cacgtgtcgc aaaaaaccca gtgactgttc
     8461 ccgctggtgt agaagtaaaa tttggaacag aggcattagt tattaagggt aagaacggtg
     8521 aattgtcttt tcctttgcat tctgatgtag ccattgaatt taatgatggc aaattggctt
     8581 ttgttgcgaa taacagcagt aaacaagcaa atgcaatgtc tggtactgct cgcgcattag
     8641 tcagcaatat ggttaaaggt gtttcagaag gttttgagaa aagattgcaa ttgataggtg
     8701 tgggttatcg tgctcaagca caaggtaaaa tcttgaatct gtctttgggt ttttctcatc
     8761 cgatcgtata tgaaatgcct gaaggtgtct ccgttcaaac tcctagccaa acagagattg
     8821 ttttaaccgg ctcggataaa caagttgttg gtcaagttgc tgctgagatt cgtgcgttcc
     8881 gtgctcctga gccttataaa ggtaaaggtg ttcgctatgt aggagaagta gtggtaatga
     8941 aagaagccaa gaaaaaataa ttgaggttca ctaatggata aacatacaac ccgactccgt
     9001 cgtgcacgca aaacccgtgc tcgtattgcg gacttgaaaa tggtaagatt atgtgtgttc
     9061 cgaagcaata atcatattta tgctcaagta attagtgctg aaggtgataa agtattggct
     9121 caagcctcta cattggaagc tgaggtgcgc ggtagtctga aatctggaag caatgttgaa
     9181 gcagctgcaa tagttggtaa acgtattgct gagaaggcta aagcagcagg tgtagaaaag
     9241 gttgcttttg atcgttcagg tttccaatat cacggtcgtg tgaaggcttt ggctgaagct
     9301 gctcgtgaaa atggtttaag cttctaaata tttggagact ttcagatggc aaaacatgaa
     9361 attgaagaac gcggtgacgg tctgattgaa aagatggtcg cagttaaccg tgtaaccaaa
     9421 gtagttaaag gtggtcgcat tatggctttc tctgcgctaa ctgttgttgg tgatggagat
     9481 ggtcgtattg gtatgggtaa aggtaagtca aaagaagtgc ctgttgcagt ccaaaaggcg
     9541 atggatcaag cacgacgctc tatgattaag gtaccattaa aaaatggtac gattcatcat
     9601 gaggttattg gtcgacatgg tgctactaaa gtatttatgc agcctgctaa agagggtagt
     9661 ggcgtaaaag ccggtggacc tatgcgtttg gtttttgatg ctatgggcat tcataatatc
     9721 tccgccaaag tgcacggatc tactaaccca tataatatcg tacgtgcaac attagatggt
     9781 ttgtctaagt tgcatactcc tgctgatatc gcagccaaac gtggcttgac agtggaagac
     9841 attttgggag ttaaccatgg ctgaacaaaa aaagattagg gttacattgg ttaaaagcct
     9901 gattggtaca attgaatctc atcgtgcatg tgcacgcggt ttaggtttgc gtcgtcgcga
     9961 gcatacggta gaggttttag atacccctga aaaccgtggt atgattaata aaatcagcta
    10021 cttgttgaaa gtggagtctt gatatgtttt tgaatacaat tcaacctgct gttggtgcta
    10081 cgcatgctgg tcgtcgtgtt ggacgcggta ttggtagtgg tcttggcaaa acgggtggtc
    10141 gtggtcataa aggtcaaaag agccggtctg gtgggtttca taaggtgggt ttcgagggtg
    10201 gtcaaatgcc cttgcaacga cgcctcccta aaagaggttt taaatcttta acagcatcag
    10261 ctaatgcaca gcttcgttta agtgaactgg aatcaattgc tgttaatgag attgatattt
    10321 tggtcttaaa gcaagcgggt ctgattgcat ctacagtctc taatgttaaa gttattgctt
    10381 ctggtgaaat ttctaaggca gttgctttga agggtattaa agttaccaaa ggtgcgagag
    10441 ctgctatcga ggctgttggt ggtaagattg aaatgtaagg tttaatattg tggctaatca
    10501 acaaacgtca tcaggttcat ccaaatttgg agatcttaag aaacgtcttt tgtttctatt
    10561 tggagcattg attgtttttc gaattggtgc ccatataccc gtacctggag ttgatgctgt
    10621 tgctttagct aaattatacg aaagcgctgg aaacggcatc ctgggaatat tgaatatgtt
    10681 ttccggtggg tcgttagagc gctttagtat atttgcaata ggaattatgc catatatttc
    10741 agcttctatt attgtacagc ttgcttctga aattttgcca tcattgaagg ctttaaaaaa
    10801 agaaggggag gctggtagaa aggtaattac gaaatatact aggtatggta ctgttttgtt
    10861 agcaattctt caaagtctag gtgttgcatc tttcgtattt cagcaaggaa ttgttgtaac
    10921 aagttcattt gagtttcatg tttccacggt agtttctttg gtaacgggaa ccatgtttct
    10981 tatgtggctt ggggagcaaa ttactgaaag gggtatcggg aacggtattt ctttaatcat
    11041 tacggcaggt attgcttcag gtattccttc gggtattgca aagctggtta cactgacgaa
    11101 ccaaggttct atgagcatgc ttacggcgtt gtttattgta tttggtgcct tattattaat
    11161 ttatttggtt gtatactttg aaagtgcaca gcggaagatt cctattcatt atgcaaaacg
    11221 ccagtttaat ggtagggcgg gtagtcaaaa tacgcatatg cctttcaagt tgaatatggc
    11281 tggtgttatt cccccaattt ttgcttccag tattattcta tttccatcta ctcttttagg
    11341 ttggtttggt tcggctgata caaatagtgt tttgcacaaa atagctggat tgttacaaca
    11401 cggtcaattg ctgtatatgg ctttatttgc agcgacagtt attttctttt gttattttta
    11461 tacggctttg gtttttagcc ctaaagaaat ggcagagaat ttaaaaaaga gtggtgcttt
    11521 tgttcctggg attagacctg gtgagcagac ctctaggtat ttagaaaaag ttgtattacg
    11581 tttgacattg tttggagctc tttatattac aactatttgt ttaattccag agttcttaac
    11641 tacggtttta aatgtacctt tttatttggg tggcacgtct ttgttgattc tagttgttgt
    11701 aacgatggat tttagtacac aaataaattc gtataggctt actcaacagt atgataagtt
    11761 aatgactcgt ttagaaatga aatcattttc tcggaaatag aattatggcg aaagaagata
    11821 ctatccaaat gcaaggtgaa attcttgaaa ctttacctaa tgcaacattt aaagtaaaac
    11881 ttgagaatga ccatattgta ttgggtcata tttctgggaa gatgcggatg cattacattc
    11941 gtatttctcc gggagataag gtcacagtag agctgacacc ttatgatcta actagggctc
    12001 gaatcgtttt cagagcaaga taaaccaata aaaggaaaat aaaatgcgtg tacaaccatc
    12061 tgttaagaaa atttgccgaa attgcaagat tattcgtcga aatcgtgtag ttcgtgtaat
    12121 ttgtactgat ctccgtcaca aacagcgtca aggttaatgg aatatttctt ttaatgtgat
    12181 tctgtgatat agtgacacac tttgccctaa aaaggaaaaa atatggctcg tattgcaggg
    12241 gtaaatatcc ctaataacgc acacatcgta attggtcttc aggctattta cggtattggt
    12301 gctactcgtg ctaaattgat ttgtgaggct gcaaatattg cgcctgatac taaagcaaaa
    12361 gatttggacg agactcaatt agatgctttg cgtgaccaag ttgccaagta tgaagtagaa
    12421 ggtgatttgc gtcgtgaggt aactatgagt atcaagcgat tgatggacat gggctgctat
    12481 cgtggcttcc gtcatcgtcg cggcttacca tgccgcggtc aacgcactcg tacaaatgcg
    12541 cgtacccgca aaggtccgcg taaagcgatt gctggtaaga aataaatttt aaggaatttt
    12601 attaatggct aaagcaaaca cagcttcacg tgtacgtaaa aaagtacgta aaaccgtgag
    12661 tgagggtatt gtgcacgttc atgcatcttt caacaatacc atcattacaa tcactgaccg
    12721 tcaaggcaat gcgttgtctt gggctacctc tggcggcgct ggttttaaag gttctcgtaa
    12781 aagtacacca tttgcagcac aagttgcagc agaagcagct ggtaaagttg cccaagagta
    12841 tggcgttaaa aatttagagg ttcgtattaa aggtccaggt ccaggtcgtg aatcctctgt
    12901 acgtgctttg aatgctcttg gtttcaagat taccagcatt actgacgtta ccccgttgcc
    12961 tcataacggt tgccgtccgc ctaaaaaacg tcgtatttaa tattggagtg atttgaaaca
    13021 tggcacgtta tattggccct aaatgtaagt tggcacgtcg cgaaggtacg gatttgtttt
    13081 tgaagagtgc gcgccgctct ttggattcta aatgtaaaat tgattccgct cccggtcagc
    13141 atggtgcaaa aaaaccgcgt ttgtcagact atggtttgca gttgcgtgaa aaacaaaaaa
    13201 tccgccgtat ttatggcgta ttagaacgtc agttccgtcg ttatttcgca gaagctgatc
    13261 gtcgtaaagg ttctaccggc gagttgctgt tgcagttgct ggaatctcgt ttggataatg
    13321 tcgtttatcg tatgggtttc ggttctaccc gagctgaagc aagacagctt gtttctcata
    13381 aggcgatagt tgtgaatgga caagttgtca atattccttc tttccaagtg aaagctggtg
    13441 atgttgtctc agttcgtgaa aaagccaaaa aacaggtacg tattcaagaa gcattgggtt
    13501 tggcaactca aatcggcttg ccgggttggg tttctgtaga tgcggataaa cttgagggtg
    13561 tgttcaaaaa catgccggat cgctcggaat tgaccggtga tattaatgaa cagctggtgg
    13621 tagagttcta ctctaaataa tgctagctca gtgagggaca gttaaatgca gaatagcaca
    13681 accgaatttt tgaaacctcg tcaaattgat gtaaatactt tttctgcaac tcgtgcaaaa
    13741 gtatctatgc agccatttga acgtggtttc ggtcatacct taggtaatgc tttgcgccgt
    13801 atcttactgt catccatgaa tggttttgct cctactgaag tagctattgc cggtgtatta
    13861 cacgaatatt ctactgttga tggtattcag gaagatgttg ttgacatttt gctgaatatt
    13921 aaaggtattg tgtttaaact ccatggtcgt agccaagttc aacttgtgtt gaagaaatca
    13981 ggttcaggtg tcgtatctgc cggtgatatt gagttgccgc atgatgtaga aattctgaac
    14041 cctggtcatg tcatttgtca tttggctgat aacggtcaaa ttgagatgga aattaaagta
    14101 gagcaaggtc gtggttatca atctgtttca ggtcgtcagg tagttcgtga tgagaaccgt
    14161 cagattggtg caatccagtt ggatgcgagc ttttcgccca tcagccgtgt tagctttgag
    14221 gttgaacctg cacgtgtaga gcagcggacg gatcttgata agttggtttt ggatatcgaa
    14281 accgacggtt ctattgatcc tgaggaagct gtacgcagtg cggcacgtat tttgattgat
    14341 cagatgtcta tttttgctga tttgcagggt acgcctgtgg aggaggttga agaaaaagca
    14401 cctcctatcg accctgttct tttgcgtccg gtggatgatc tggaattgac agtacgttca
    14461 gctaattgtt tgaaagctga ggatatttat tatattggcg atttgattca acgcactgaa
    14521 accgagcttc ttaaaacgcc gaatttggga cgtaaatctt tgaatgagat taaggaagta
    14581 ttggcatcta aaggtttgac actgggttct aagttggaag catggccacc tgtaggcttg
    14641 gaaaagcctt aatgaagaat taaaggataa ttgatatgcg tcatcgtaat ggcaatcgca
    14701 aattaaaccg taccagcagt catcgtgctg caatgctgcg taatatggcg aattcattat
    14761 tgactcacga agctattgta acaactctgc ctaaggccaa ggaattgcgc cgtgtagtag
    14821 agccgttgat tacattgggt aaaaagccgt cattggcaaa ccgccgtttg gcatttgacc
    14881 gtactcgcga ccgtgatgtt gtagtaaaac tgtttggcga tttgggtcct cgttttactg
    14941 ctcgtaacgg tggttatgtt cgggtgttga aatacggatt ccgtaaaggt gataatgcac
    15001 ctctggcact ggttgaattg gttgacaaac cggctgctga gtaattttag tcatataacg
    15061 ccatctgccg aaaagcaggt ggcgttattt ttgcaatatc tgataggtaa tagggtattg
    15121 gctatcatgt ttaaaatatt aattgaatag ctaaggtttg cgcggtaaac ttacatcatt
    15181 aaaaaattct atgatggttt atataatgaa tgctttcgat ataaagtcga caaagatgga
    15241 cgtattgtct atatctttgc atacgtcaga cttgtttgat ttggaagatg tgctggtcaa
    15301 attgggcaag aagtttcaag agtctggtgt tgttccattt gtgctggatg ttcaagagtt
    15361 tgattatccc gagtctttgg atcttgctgc attggtttcg ttgttttcaa ggcatggtat
    15421 gcaaattttg ggtctgaagc attctaatga acgttgggct gctgcggcta tgaagtatca
    15481 tttgctgttt tgtctgtctc attcggaaaa tgttaaagaa ctgggtcagg ttgaggtgca
    15541 gaacacggag gatggtcaga aagcaaggaa aacagtattg attacatccc ctgtccgtac
    15601 cggtcagcag gtttatgccg aagatggcga tttgattgtt acgggggcgg tcagccaggg
    15661 ggcggaattg attgcggatg gcaatataca tatttatgcg ccgatgaggg ggcgtgcttt
    15721 ggccggtgcc aagggtgata cttctgcccg catatttatc cactccatgc aggcagaact
    15781 ggtttctgtg gcgggtattt accgtaattt tgaacaggat ttgccgaacc atctgcacaa
    15841 gcagccggta cagatattgt tgcaggataa ccgattggtt atcagtgcaa ttggctcaga
    15901 gtaattgttt gatattttaa aaaggaaata ttgtggcaaa aattattgta gtaacttcag
    15961 gtaagggcgg tgtcggtaaa acgactacca gtgccagtat tgcgacaggt ttggcattac
    16021 gcggatataa aactgcggta attgattttg atgtgggctt gcgtaacctc gacctcatta
    16081 tgggttgtga gcgtcgtgtc gtttatgacc tgatcaatgt cattcagggg gaggcgacgc
    16141 tcaaccaagc tttgattaaa gataaaaatt gtgaaaacct gtttattttg ccggcttccc
    16201 agactcggga taaagacgct ttgacacgcg agggcgtaga aaaagtgatg caggagctgt
    16261 ccggcaagaa aatgggcttt gagtatatta tttgcgactc tcctgccggt attgagcagg
    16321 gtgcattgat ggcgttgtat tttgctgatg aagccattgt aacgaccaat cctgaggttt
    16381 ccagtgtgcg tgactccgac aggattttgg gaattttgca aagcaaatcc cataaggcag
    16441 agcaaggcgg ttcggttaaa gaacatctgt tgattacgcg ttattctccc gaacgagtgg
    16501 caaaaggcga aatgctgtct gtacaggata tttgcgatat tctgcatatt cctttgctgg
    16561 gtgtgattcc tgaatcccaa aacgtcttgc aggcatccaa ttccggagaa ccggtcatcc
    16621 atcaggacag cgtggcggct tccgaggcat ataaggacgt tattgcccgt cttttgggcg
    16681 agaaccgtga aatgcgtttc ttggaagctg agaaaaaaag cttcttcaaa cgtctgtttg
    16741 gaggataagg tatgtcatta atcgaatttt tattcggcag aaagcagaaa acggcaaccg
    16801 ttgcccgcga ccgccttcaa atcatcattg cccaagagcg cgcccaagaa ggtcaggctc
    16861 cggattacct gccgacttta cgtaaagagt tgatggaagt cctgtccaaa tatgtgaatg
    16921 tttcattaga caatatccgt atttcccagg aaaaacagga tggtatggat gtgcttgagt
    16981 tgaacattac tttgccggaa cagaaaaagg tataggacat gaccttaacc gaattgcggt
    17041 acatcgtcgc agtcgcccaa gaacgtcatt tcggcagggc ggcgcggcgt tgttttgtca
    17101 gtcagcccac tttgtctatt gccattaaga aattggaaga agagcttgcc gtctctttgt
    17161 ttgaccggag cagcaacgat attattacga ccgaggcggg ggaacgtatc gttgcacagg
    17221 cgcgtaaggt gttggaagag gcagagctta tcaggcattt ggcaaatgaa gaacaaaacg
    17281 agctggaggg tgcgttcaaa ctcgggctga tttttacggt tgcgccgtac ctgctgccga
    17341 aactgattgt ctcgttgcgc cgtactgcac cgaaaatgcc tttgatgttg gaagagaatt
    17401 acacgcatac tttgaccgag tcgctcaaac gcggggacgt tgatgcgatt atcgttgccg
    17461 aaccgtttca agagccgggc attgttaccg aacccttgta tgacgaaccg tttttcgtga
    17521 ttgtcccgaa agggcattca tttgaggaac tggatgccgt ttcgccccgg atgctgggtg
    17581 aggagcaggt tttgctgctg acggaaggca actgtatgcg ggatcaggta ctctcaagct
    17641 gttccgaatt ggcggcgaaa caacgtatac aggggttgac caatacattg cagggcagct
    17701 cgattaatac aatccgccat atggttgcca gcggtttggc aatcagcgtg ttgccggcaa
    17761 ccgcactgac cgaaaacgat catatgctgt tcagcattat tccgtttgaa ggtacgccgc
    17821 caagccggcg ggtcgtattg gcgtaccgcc gcaattttgt ccgtccgaag gcgttgtcgg
    17881 cgatgaaggc ggcgattatg cagtcgcagc ttcacggggt aagttttatc cgcgactagg
    17941 cgcaggcatt gttttcaaaa cgccatttcc ctgagccgac aacacggtat gccaagatat
    18001 tgccgtcatc atcgattttg agtatagcat cgccacggaa actgccgtcc tgaagatatt
    18061 cgacttttgc atcactgtga atgttttcat cagtgccgat gcaatgccat gtatagtgga
    18121 ttaacaaaaa ccagtacggc gttgcctcac cttgccgtac tatttgtact gtctgcggct
    18181 tcgtcgcctt gtcctgattt ttgttaatcc actataaaag aggccgtctg aaaaacattt
    18241 ttcagacggc cttgtttatt caatcaaatc agtctttcaa cttcgccaac tgattttgaa
    18301 cttttgccat tttgtcttcc aattccgcca aatcggcttt gtctttttcc accagatgcg
    18361 caggggcttt ttcggtgtag ccgggtttgg agagtttggc gttgagtttg tccaaggctt
    18421 tttgcagctt ctcggcttct ttgctcaaac gggcggtttc ggcggctttg tcgatttcga
    18481 ctttcaacat caggcgcgcg ccgttgcaga cggcgacggg cgcgtcttcg ctttcgggta
    18541 gggcggcgac ttgctgtgct tcggtcaggc gggtcatcat cggcaggtat ttgaggtagt
    18601 ccgccaagtc gtccgtgctt tcgacaaaca gcggggcttt cacgttgggc tggatgccca
    18661 tttcgccgcg caggttgcgg actgcgccga tcaaatcctg caacacggtc atttgcccga
    18721 atgccgtctg aacaatctcg ccgccgtcgg cttcggggaa gcgggcgagc atgatgctgt
    18781 cggcggtttt cgcgtcgcac ataggggcga cggtttgcca cagttcttcg gtgatgaacg
    18841 ggataatcgg gtgcagcagg cgcagggcgg cttcgagtac gcgcaataag gtatggcgtg
    18901 tggcgcgttg gcggctggcg cagccggttt gaagctgcac tttggcaagt tccaaatacc
    18961 agtcgcaata gtcgttccac acgaagctgt acagggtttc cgccgccaaa tcaaagcggt
    19021 aggtttcgta ggcttgcgtt acttgctcga tggtctgatt cagacggtct acaatccaca
    19081 tatcggggaa ggaatagccg cgcggttcgg tggcggttgc gccgtagccg cagtcttggt
    19141 tttcggtgtt catcaagacg aagttggtgg cgttccagat tttgttgcag aagttgcggt
    19201 agccttcggc gcgtttgaag tcgaagttca cgctgcgccc caagctggcg tagctcgcca
    19261 tggtgaaacg caatgcgtcc gcgcccatac tcggaatgcc ttcgggaaag agttttttgg
    19321 tggcttcttc cactttcggc gcggtttcag gtttgcgcag gccggtggta cgttttacca
    19381 gcagcttgtc caagccgatg ccgtcaatca aatccacagg atcgatgacg ttgccttcgg
    19441 atttggacat ttttttgcct tcgtggtcgc gcacgatgcc gtggatgtac acgtctttaa
    19501 acggtacttt gccggtaaag tgggtggtca tcataatcat gcgcgccacc cagaagaaga
    19561 tgatttcgta gccggttacc aagacgttgg acggcaggaa ggctttgagt tcgtcggttt
    19621 cagacggcca gccgagcgtg gaaaacggca cgagcgcgga ggagaaccat gtgtccaata
    19681 cgtcttcttc tcgggtcaag cctgttttgc cggcttgttt ttcggcttcc acctgattgc
    19741 gggcgacgta aacattgcct gcttcgtcgt accacgcggg gatttgatgc ccccaccaca
    19801 gttggcgcga gatgcaccag tcttggatgt tgttcatcca ttggttgtag gtgttgaccc
    19861 agttttcagg gataaagcgt accgcgccgc tgtcgacggc ttttttggct ttgtcggcga
    19921 ggctcaagcc tttgaattcg ctgtcaggct cgccgccgtt tggcgtggcg gacatggcga
    19981 caaaccattg gctggtcagc atcggctcaa tcaccgaacc ggtacggtcg cctttcggcg
    20041 tcatcagcgt gtgcgctttg atttcgacca agaaaccttg ttcctgcaaa tcggcaacca
    20101 tttgtttgcg cgcggcaaag cggtccaaac ctgcgtattt ttcaggcagg gcaaagcctt
    20161 gttgcgcttc gcctttgaag ttgaacactt cggcgtttgc cagcactttg gcttccaaat
    20221 cgaacacatt aatcaggcgc gtatcgtggc gtttgccgac ttcgtagtcg ttaaagtcgt
    20281 gcgcaggcgt gattttcacg cagccggtac caaagtcttt ttcaacgtat tcgtcggcga
    20341 taaccggaat ggtgcggccg gtcagcggca ggattaattc cttgccgatt aagtgggtat
    20401 aacgttcgtc ttcgggattg acggcaacgg caacgtcgcc caacagcgtt tcgggacggg
    20461 tggtggcgac gataacggct tcggcaggat tgtccgccag cggatagcgg atgtgccaca
    20521 tagagccttg ttcttccacg ctttctactt ccaaatccga caccgccgta ccgagcactg
    20581 gatcccagtt taccaagcgt ttgccgcgat aaatcaagcc ctgttcatac aggcgtacga
    20641 acacttcggt cacggtttcg gcgcgtacat cgtccattgt gaaatactcg cgcgtccagt
    20701 cggcagaaca gcccacacgg cgcatctgct gcgtaatcgt gccgccggaa acttctttcc
    20761 attcccacac tttttccaag aatttttcac gacccaagtc atgacgggac acgttttgcg
    20821 cggcaagctg acgctcgacc acgatttgcg tggcgatgcc cgcgtggtcg gtgccgggaa
    20881 tccaggcggt gttgcagcct ttcatacggt agtagcgggt caggccgtcc ataatggttt
    20941 ggttgaaggc atgacccatg tgcagcgtgc cggttacatt gggcggcggc agttggatgg
    21001 aaaaagacgg tttggtcaaa tccatatcag gttggaaata gccctgctct tcccagtttt
    21061 gataatgttt ggattcgatt tcggctggat tgtatttgtc taacatgatg gaactttgtg
    21121 aaattaaggt tatttttgat gtgcggatta taacgcaaaa aggccgtctg aatcatttca
    21181 gacggccttt ggcatacagg ttttaaaaat ggaacaatac caggctgacg gcaatcaccg
    21241 ccatgcccat tgtcaggccg taaacggttt catggccgtc tgaatagcgt ttggcagccg
    21301 gcagcagctc gtccaacgcc aaaaacacca tcacaccggc tatcacgccg aataccgaac
    21361 caaacacggc aggcgacaaa aacggctgca aaaccaaata gcccaaagcc gcccccaacg
    21421 gctcggccaa gccggatagc agacacgccc acaccgtttt cttacggctg cgggtggcaa
    21481 aataaaccgg cgcggcgatg gaaatgccct ccggaatatt atggatggca atcgccaagg
    21541 ccaaaggcat cccgactgct ggattttcca atgtggcaaa aaacgtcgcc aagccttcgg
    21601 ggaaattgtg cgcagtaatc gcaaacgccg ccatcatgcc gactcgcgcg atatggcggc
    21661 gtttgctttc ttgaaacgac gggtcttgcg cgtctaaagt ttcatgcggg ttcggcacca
    21721 gacggtcaat cagcgcaatg ccgcccatcc ctgccaaaaa tgccatggtc gccgccgcaa
    21781 acgcgtggtc tttatcataa atttcagcga acgcctcgct ggacttactg aaaatctccg
    21841 tcagggaaac atataccatc gcaccgccgg caaacgccaa accaaacgac aacacacgcg
    21901 gattgggcgt tttggaaaac atcaccaagc cactgcctaa tacggtaaac aaaccggcag
    21961 ccaatgtgat ggaaaaggca acggccaaat tggacatcga aaaatcgggc atgagaaaac
    22021 ctgcgctaaa agctgggaca ggttcagact aacacttttt aatgtatatg ataatagtta
    22081 ttatttattt tattgattgg atacacggat tttgaaacaa aaggccgtct gaaaaatgat
    22141 tttcagacgg cctttaaatt tgaaatgccg ctaaacctta gtgctttcca gcttaagcct
    22201 gataacgcga caggctcaaa tcgtcgctgc ggatttcggt gtctttgccg ctcacgatat
    22261 cggcggttaa ttttgccgaa cccagcgaca tggtccagcc taaagtaccg tggccggtat
    22321 tcagaaacag gttgtcaaag cgggtgcgac cgattaacgg cgtgctgtcg ggcgtcatcg
    22381 gcctgaggcc gctccagaac gatgcttggc tcaaatcgcc gccttccggg aacaagtcgt
    22441 tgacgaccaa agccaaggtt tcgcggcgtt tttcgggcag tttgatttcg tagcccgaca
    22501 attccgccat accgccgacg cggattctgt tgtcaaagcg cgtgatggcg actttgtagc
    22561 tttcatctaa aacggtggac accggtgcgc cgtcggaatt tgtgaccggc agggtcaagg
    22621 aatagccttt gacgggataa atgggcagat tgagatccaa ctgcgccaaa accgtcctgc
    22681 tgaaacaacc gagcgcgcag acaacggcat ctgcttcaaa ccgccctgtt tcggtttcaa
    22741 cggttttgat gcgcagcccg ttgtggtcga tgcggctgat ggtttggttg aaatgaaacc
    22801 gtacgccctt ttcctgacac aatttgtata ggttttcagt gaagaggcgg cagtcgccgg
    22861 ttgcatctgc aggcaggtgc aggccgctgg caattttggc ggtaacgcgt gccagcgcag
    22921 gctcaaattc tgcacattct tcgggtttca gacggcggta cggcacgccg tagcgttcca
    22981 aaacggcaat gtcttgttct gccgcttcga cttctttggt ttggcggaaa atctgcaacg
    23041 tccctttttt gcgttcctca aaattcatgc cggtttgcgc ttcaaaacgg cggaacattt
    23101 cacggctgta ttcggaaatc ctgaccatgc gctctttatt ggtttgatag tgcgctgccg
    23161 tgcagttttg cagcatttgc cacagccatt cgatttgata caggctgccg tcggggcgaa
    23221 acagcaaagg cggatggctt ttaaacagcc atttcagcgc tttggtcggg ataccgggtg
    23281 cagcccaagg cgtggtatag ccgtaagaaa gctggcctgc gttggcaaaa ctggtttcca
    23341 tcgccacacc ctcggcgcgg tcgatgaccg ttacttcatg tcctgcctct gccagatacc
    23401 acgcggaaga cacgccggca acacccgcac ctaaaacaag cactttcatg tttctccctc
    23461 cggctttttc aaaacagact taatatgccg tgccgtctga atattcggat tcagacggcc
    23521 tcggatatta atgcggcaat tcgccgtttg tgattttttg tttgaagtcg cgcgtttcat
    23581 tgacgatgac tttcgccatc aataaaagtg caatcaggtt gggcaatgcc atcaagccgt
    23641 tgaatgtgtc cgaagccagc cacaccaaat caaggctcaa cacggtacct agcataacgg
    23701 aagaaacata accgacgcgg tacaaaccgg caaatttctc gccgaaaaca tacaccgcgc
    23761 atttttcgcc gtaatagcac cagcccaaaa tggttgagta ggcaaagaaa atcaggccga
    23821 tggtgacaat ccagccgccg atgccgggca gcattttttg gaatgtgacg gttgtcagtg
    23881 ccgcgccgct cacttcaggt ttgacaaact cgccgcccgc gccgagcagt cccattacca
    23941 acacgatgcc ggtaatcgag caaacgacga tggtatccaa aaacgtaccg gtcatagaaa
    24001 ccaaggcctg acggacggga tggtcggttt tcgcggctgc ggcggcaata ggcgcagaac
    24061 ccatacctgc ctcattggag aacacgccgc gcgccacgcc gtagcggatg accgtaccga
    24121 tagcaccgcc cgccactgcc tgcgcgctga acgcatcgga gaaaatcagc ttgacggcag
    24181 gcatcagtgc atcggaatta atcgcgataa tggaaagacc gcccaacaca taaaacaccg
    24241 ccatagcagg cacgatgaaa gaagcggctt tggcgatgcc tttaatacca cctaaaacga
    24301 caacggcagt cagaacggtc aacgtaatgc cggtataggc aggttcgata ccgaagctgg
    24361 tttgcaccgc ctgtgcaacc gagttggact gcaccgagct gccgataccg aaggaagcga
    24421 atgtgccgaa cagcgcaaat gcaacggcca tccatttcca gtttttgccc aagccttttt
    24481 cgatgtaata catcgggccg ccggacattt cgcctttgga attggtgacg cggtatttca
    24541 ccgccaacac gccctcgccg tatttggtgg ccatgccgaa aatggcggtc atccacatcc
    24601 aaaataccgc gcccgggccg ccggttacca ccgcagtcgc cacaccggcg atgttgcccg
    24661 taccgatagt ggcggacagc gcggtcatca acgccgcgaa atgggaaata tcgccttcgt
    24721 ggtcttcgcc gtcttcatgc ttctttgacg gcacaaacgc ctgtttcagt gcataaccca
    24781 acatagtgaa ctgcaagcct ttcagcaaaa cggtcagcaa aatgcccgta ccgaccagca
    24841 ataccaacat gacagatccc cagacccagc cgctgatggt ttcaaaaaag gctttgagat
    24901 tgtctaaaaa caattgcatg gctttctcct ttgtctgttt tatttttaaa acaccacttt
    24961 tgtagtgtcc tgtaatttca gcacagaata ttcaataaga caatatgttc ttttgaaaaa
    25021 tacttttggt tttttcgccg aaagcaggtc ggttcaagct gcggaaattg tttgcaattc
    25081 tttaaaagca gcggcggagg tcacaatgaa atgtccgaat ggggatgtgg ccggcggcag
    25141 aaatcatcaa tgctgccgac tgccatactt ctgaaatcta caaaatgatg catcgatcaa
    25201 acaatatacc gctttaaaaa aaccgatgcc gtctgaaacg ctttcggggt ttcagacggc
    25261 atcaaaaggg tacggtcagc ggatgatgcc gcgcgccgat tgtgcgaaaa agtctcggaa
    25321 tacggcaagc tcggcttggg tttcggcgcg gcggagaatg tctgccttgg cttcttcaaa
    25381 cggaatgccg cgatggtaga gggttttgta cacgtctttg acggcggaaa tctgctctgc
    25441 ggtaaaaccg ttgcggcgca tgccttcgct gttgagcccc gccggttcgg cgcggtagcc
    25501 cgatgccata aagtagggcg gtacgtcttt gtgtacgcct gcggcgaagg cggtcatggc
    25561 gtagtcgccg atgcggcaga attggaaaac cagcgtgtag ccgcccaaaa cgacgtagtc
    25621 gccgatggta acgtgtccgg caagcgaggc gttgttggcg aaaatggtgt ggttgccgac
    25681 cacgcagtcg tgcgcgaggt ggcagtacgc cataatccag ttgtcgtcgc cgatacgggt
    25741 ttcgccgatg ccagtaactg ttccgagatt gaacgtcgta aattcgcgga tggtgttgcc
    25801 gttgccgata atcagccggg tcggctcgtc gcggtatttt ttgtcctgcg gaatttcgcc
    25861 gaggctggca aattggaaaa tgcggttgtt ttcgccgatg ctggtgtggc cgttgatgac
    25921 ggcgtgcggg ccgatttcgg tattcgcgcc gatttggacg ttggggccga tgacggtgta
    25981 cgcgccgact ttgacgctgg agtcgagttc ggctttgggg tcgatgacgg cggtcgggtg
    26041 gatgagggtc atgtttttcc tttcctgtcg tgttgccgcg aagatgcgcg acggcaacag
    26101 gtcgtctgaa aactttcaga cgaccttttt ctgaacactc aaaccacgcg tttggcgcac
    26161 ataatcacgg cttcgacggc gacttgcccg tccactttgg caacggcgtt gaatttgccg
    26221 atgccgcgcc ggctggtcag cagctcgact tcaaagacga gttggtcgcc ggggatgact
    26281 tggcgtttga aacgggcttc gtctatgccg gcgaagaaga agaattcgtt ttctttgcgc
    26341 ccgccttcgc tcaaaatcgc caacgtgccg cacgcctgcg ccatcgcttc gatgatgagt
    26401 acgccgggca tcacgggcag gtcgggaaaa tggccttgga actggggttc gtttatggtg
    26461 acgtttttaa tcgcggtcag ggttttcatc ggctcgaagg cggtgatgcg gtcgagctgg
    26521 agaaacggat agcggtgggg gatgagtttt tggatgtctt tggcttcgat ggggagttgt
    26581 acgtccatgt ctgtcgtatt ccttgaataa agtcggtttg gttatttgct gtcttgaccg
    26641 gcatctgaaa gctgctgctc cagtgttttg agccgtttgt tcatttcgct taagcggtgg
    26701 atgtaaacag cgttgcgcgc ccattcttta tgggtggaca tcgggaagat gccggcgagg
    26761 tgtttgccgc tttcggtaat gctgtgggtg acggacgtgc cgccgccgat ggtggttttg
    26821 tcggcgattt cgatgtgtcc gaccgtaccg acgccgccgc cgatgatgca gtagctgcct
    26881 atggttacgc tgcctgagat gccggttttg gcggcgatga cggtgtgcga accgattttg
    26941 cagttgtgtc cgatttggac ttggttgtcg attttggtgc cgttgccgac ggtggtgtcg
    27001 ctcatcgcgc cgcggtcgat gttggtgttc gagccgattt ccacgtcgtc gcccagcgtt
    27061 accgcgccgg tttgcgggat tttaaaccat gaatcgccgg caaaagcgag tccgaaaccg
    27121 tccgcgccga tgaccgcgcc gctgtggatt tcgacgcgtc tgcccagtgt gcagccgtaa
    27181 taaacgacgg cgttgggatg caggacgact tcgtcgccca gtttgcaatc gtgttggacg
    27241 acggcgtttg ccaagatgcg gcagccttcg ccgagcacgg tgtttgcgcc gatgtaggcg
    27301 ttcgcgccga tttcgcagct tgcgggaacg gtggcactcg gttcgacgac ggcggtcgga
    27361 tggatgccgc cgcgcgcttt gacgacgggt gaaaacaggc gggcgacttt ggcgaaatag
    27421 agataggggt cgtcggcgac aatcaggttg cgcccttcaa atccgtctgc cgctttggcg
    27481 gaaacgatga ccgcgcccgc gctgctgtcg tggacttcgg ctttgtattt cggattggca
    27541 aggaagctga tgtgttccgc ctgcgcgtct gcgagcgggc gcacggcggt aacggaaatg
    27601 tcctcgccgc gccattcgcc gccgagctgt gcggtgattt gggacagggt gtaggtggcc
    27661 ggaatcatgg ttttcctgtt cgatatgccg tctgaaaggg tcagcgggcg ttcatttctt
    27721 taatgacgct gtcggtaacg tcgtattggg tgttgacgta aatcacgttc tgcaaaatga
    27781 catcgtaacc ttcctgtttg gcgattttga cgatgacgcg gttggcgttt tgctggaggg
    27841 aggcaaactc ttcgttgcgg cggaggttgt agtcttcttc aaactgcgcc tgttttttgc
    27901 ggaacgctgc gaccagcccg cgccattttt cttcggcttg cgcctttttt gcgtttctga
    27961 gtttgccttc ggcaagctgc ctttccaaat ccagaccttc gcgttgcagt ttttgcaatt
    28021 cgtcctgacg agcggaaaat tcgctgtcca gcgttttttg aatcttgcgc gcctgcttgg
    28081 attcgaggta gatgcgctcg gtgttgataa agccgatttt ttggaaggtg tcggcgtgcg
    28141 cgcctgcggt gcagcacaaa ccgatcagag ccgcggcaaa cgcgcgggtc aaacgggtca
    28201 tggtaaaact ccttcgaatg ttgccgcgaa atgccgtctg aagggcttca gacggcattt
    28261 gcgggattag aacgtcgtgc cgagttggaa ttggaagcgt tggatttcgt cttccggttt
    28321 tttcttcagc gggtaggcgt agctgaattt catcgggcct aaaggcgaga gccaggtaac
    28381 cgcgccgccg gcggaatagc gcaattcgtt ggtaaaggtg gatttatggg tattgccggc
    28441 gccgtaaatg ttttgaaccc tgccgccggt cgcggaactg ctgttgtcgt cgtaggtttt
    28501 gccgtcccac acgctgcctg cgtcggcaaa caggctcagg cggacggtgc gcgcgtcttt
    28561 cgcgccgggc atcgggaaga gcagctcggc ggagacgttg gcttttttgt tgccgccgta
    28621 gctgattttt tcgccgtatt cgtcatacac tttcggaccg agcgtgccgc tttcgtaacc
    28681 tcgtaccgaa cccaggccgc cgccgtagaa gttttcaaag aaggggattt ctttggtttt
    28741 gccgtagccg ttggcatagc cgatttcgcc gccaagcatc agtgtgaagg ttttgctcag
    28801 ggggaagaac caggtttggt tgtgggtggc ggagtagtat tgcagtttgc tgccgggcag
    28861 ggcgatttcg gcgttcacgc ccgtcaggta gccgcgcgtc ggccataacg cgctgtcggt
    28921 tttgttgcgc ccccagccga cggtaccttt gtacagccag cctttgaagc tgccgtctgt
    28981 gccgtcggtt ttgccgtatt tcttgataaa gtcggcatag tgtttgggcg ctttgttgta
    29041 ggtgttgacg gtcaggtgtt ctgccaccaa accgaaattc acgcggtcgt attcggtaac
    29101 aggcacgctc atgcggatgc ctgcgcctgc cgtggtggtt ttatattgtt tgatgctggt
    29161 cgatgctttg cgcgggtcga aggcttttcc gtaaacatcg tagcccaggc tgaccccgtc
    29221 tgccgtgaag tacgggtcgg taaacgacag cgagccgttg agcgtggttt tgcttcgtga
    29281 ggcgcgcagg gcggccgact tgcccgtacc gaacaggttg tcttgggaaa cgcctgcgga
    29341 catgaccagg ccggtatcct gtacccagcc cgcgctcaag tcgagcgagc cggtggaacg
    29401 ttcggtcagg ctcatgttca aatcgacttt gtcgggtgtg ccggcaagcg ggacggcatc
    29461 aaactgtacg ttgtcgaagt agcccaaaag ctcgacgcgc tctttggagc gttgcagctt
    29521 ggaggtgtcg taaggcgcgg attccatttg gcgcaattcg cggcgcacga cttcgtcgcg
    29581 ggttttgttg ttgccggtga tgtggatttc gttgacgtag attttccggc ccggttcgat
    29641 gtgcaggacg aaatcgacgg ttttggtttc ggcgtttggc agcggctgta cgctgatttc
    29701 gctgtatgcg tagcctgccg agcccatgcg gttctgaatc tcacccaaaa cggcggtcat
    29761 ctgctggcgt tcgtaccatt tgccgggctt catggtcagc agtttttcca gttcggcttt
    29821 ggggacttcg ttggtgtcgc cttcgatgga gactttgccc caacggaaac gttcgccttc
    29881 gtggacggtg attttgatgg tctgcttggt tttgtcttcg ttggtttgga tgtcggtatc
    29941 gaggatgcgg aaatcgaagt agccgttgtt ctggtagaag tcggttactt tttccatgtc
    30001 ttgggcaaat ttctgctcgt tgaattggtt gcttcgtgtc agccatgtcc aaatgccgcc
    30061 ttcggtcagc gacatctgcc gcatcagttt gcggtcggaa tagacttggt tgccttcaaa
    30121 ttcgatgtcg gtgattttgg cggatttgcc ctcgtcaatc gtgatgtcga tgtcgacgcg
    30181 gttgcgggcg agtttggtta ctttgggcgt gatttggata ttgagtttgc cgcgcccgag
    30241 gtattcttct ttcaggccgg cgactgcctg attgagtgtc gcctgattaa agtattgcga
    30301 ctgcgccagc ccgaacgatt cgagattttt cttaatggcg tcgttttgca gcatttttgc
    30361 gccggtgatg ttgagcgagc cgatggtggg acgttcgatg acggtcagca ggagctgtcc
    30421 gtccgcggat tcaacgcgta cgtcgtcgaa gaacccggtc gcgtacaggc ttttgatgat
    30481 ttccccaccg cggccgtcgt tgtaggcatc gccgattttt acgggcaggt agttgaatac
    30541 ggtactcggt tcggtacgct gcaagccttc gatacggatg tcttggatgg taaagtcggc
    30601 aaatgccaaa ggcgatatgc ccaagaccat cagtgcggaa gcaatctgtt tcagtttcat
    30661 cataagttcc ttgtggtgcg ttatgctttc agacggcata acgaaacgtg aaattcagcc
    30721 aagcaggcgg gtaatgtcgt tgaaaaaagc gattgccatc atcagcatca tgagggcaag
    30781 cccgaagcgc aaaccgatgt tttggacgcg ttcgcccaaa ggtttgccgc gtatccattc
    30841 ggcagtataa aacacgaggt ggccgccgtc caaaacgggg acgggcagca ggttcagcac
    30901 gccgaggctg atgctgacca gtgccaaaaa ttccaaataa ctttgcaagc cgagttcggc
    30961 ggactgtccg gcaatgtcgg caatggtcag cggtccggaa atatggctga cggaggcgtt
    31021 gccgctgatt agtttgccga aaaatttgag ggttgtccac gagtgggaaa cggttttttc
    31081 ccagcccatg ccgaatgcgc ggacaacaga cggacggtag ctgcggcgga tttgcgcgtc
    31141 ccacgccctg tccggctgcg gacggaggcc gacgcgcccg atcagggtgt ggtcggactg
    31201 ttcgacagta tcggggcgga tgtcggcggt atgggtttgt ccggcgcgtt cgtaggttaa
    31261 ggcaattttc ctgcccgggc tttgacgggt caggtttgcc cattcttgcc atgaggtaat
    31321 gggtttgccg tcggcggcag tcagcctgtc gcccggtttc aggcctgctt tttcggcggg
    31381 gctgcctttt tccacgccgc cagcaacggt tgtgatttta aagggcatca gtccgatgta
    31441 gccttggttt tttgcgattt taccggcttc cggcgtgctt gcggcatcga tggtgcggac
    31501 ggtttgcgcg cccgatgccg tctgaacgcc gacggcgact ttgccggctt cgaggttgag
    31561 gacgatttca gtttgcgcgc tgccccaatc tgcaacgggt gtgccgttga cggattgtat
    31621 tttgtcgccg ctttggaagc cggcgcgggc ggcaatggtg tcgggttcga ctgtgccgac
    31681 gtaggggcgc agttcggtta cgccgaagga aaagctcagt ccgtacagca aaaccgccag
    31741 tgcgaggttg gtcagtgggc cggcggcgac gatggcgatg cgcttggcgg ggtgttgttt
    31801 gtcaaaagcg tagggtaaat cggcttctga tacttcgcct tcgcgcgtat cgaccatttt
    31861 gacgtaaccg cccaacggaa tcggggcgag gcaccattcg gtgtcgccgc gctttcgggt
    31921 gaaaaacggt ttgccgaagc cgacggaaaa gcgtacgact ttgacgccgc acaatctggc
    31981 aacgatgtag tgtccgaact cgtgcaggct gaccaaaatc aggatggcga agataaaagc
    32041 tagaagggtg tgcaaatggt tttcctttga taacggtgtt cggacggcat cagcgcagtg
    32101 tgccgataaa tgcccgcgct tgtgcgcgtg tccgggcatc ttgcgccaag agccccccta
    32161 tgtcgcctat gccgtctgaa aagtcttgtg cgagacaatg ggcgacggtt ttggcaatgt
    32221 cggtaaactt aatctgcccg tccaaaaagg cggcgacggc ggcttcgttg gcggcgttca
    32281 atacgcaggg cgcggctccg cctgcgttca tggcttcata ggcgagcttc aggcagggga
    32341 agcggtcaaa gtcgggcttt tggaaggtca gcgcggacaa tgcgtcgaaa tccaggtcgc
    32401 cgacacccga atcgatgcgc tcgggcaaac ccaaacaata agcgatgggc gttcgcatat
    32461 cgggattgcc cagttgcgcc aacacggagc cgtcgcggta gcgcaccatg ctgtgtatca
    32521 cggattgcgg atggatgacg acttcgagtt tgtcgggcgg acagttgaac agccaatgcg
    32581 cttcaatcag ctccaaacct ttgttcatca tggtggcgga atcgacggag attttgcgtc
    32641 ccatacgcca attggggtgt ttgaccgctt ggtcgggcgt aatgctgtcg aacgtgttta
    32701 aatcggcggt cagaaacggg ccgccggaag cggtcaggat aatcgaagcg atgccgtgtt
    32761 cgttcagacg gccggcgtaa tcgcgcggca aaacttggaa aacggcgttg tgttcgctgt
    32821 cgacgggcag cactgccgcg ccgtttgcac gggcggtttc cataaacaac gcgccggaaa
    32881 ccaccagcgt ctctttgttc gccagataaa tggttttgcc tttttgcgcc gctgcgagcg
    32941 cggaaggcag ccccaccgcc ccgacgatgg cgcacatgac accgctgact tcgtcggcag
    33001 aggcaacgtc aaccaatgcc tgcgcgccgt gtaaaacctg cgtcgccgtg ccgtcgcctt
    33061 tcaacaaagc ttcaagccgg gcggcgtgtt cggcatcggc aacgacggca tattcggggc
    33121 ggaacgtccg gcattgcacc gccagtttct cgacctgctt atgccctgcc agcgcgaata
    33181 cgcggaattt ttcggggtgg agggagacaa cgtccagcgt gctttcgcct atgctgccgg
    33241 tactgcctaa tatggtcagg acttgtggtg tcataatggg gataacttta taccggatgc
    33301 cgtctgaagc gttttcagac ggcatagaat caatttaaaa ccgacatcat cgccgcatag
    33361 acgctgataa cggcaatcag gctgtcggta cggtcgaaca cgccgccgtg tccggacagc
    33421 aggttgctgc tgtctttaat gcccgccgcg cgcttgagcc agctttccaa caggtcgccg
    33481 catacgctga caacggtcag caccaaaccg attaacacgg tatcgaacca gcctgtatcg
    33541 aatgccagcc agccggcact tcgtacggcg gtcatgtaca ctgccacgca aaccgcgccg
    33601 ccgattgcac cttcccagct tttgccgggg ctgattgccg gtgcgatttt gtgtttgccg
    33661 aacgccttgc cgctgaaata cgcgcaaata tcggcaaccc acaccaaacc catcacggcg
    33721 agcagcggca gggcatcatc gggatgcggg cgcagggata cgagcgcgaa ccaaaacggc
    33781 atgaccagaa gccagccgac ggcataaacc tgccaaccgc cgttgagcct ccatttgaat
    33841 ctcaaccata aaggcataac ggcgagccaa aatgccaaaa caacatacca aaccaaatta
    33901 ggcagcatcc agccgcccgc ataggcaacc acgccgaaaa ccaaggttgc ggcgaggtaa
    33961 tggttggttt taattttgca caaaccgccc atacgggcat attcccacaa ggcaatcagg
    34021 gcaatcagtc cgcaaaatgc agcccacaac cattgcggcg cgtaaaacag catgcccagc
    34081 atcagcggca gcagccacat ggcggttatt acccgttgtt tcagcatatt cagttccttt
    34141 gctgttcgat aggcagttgc tcggaggtgc gtccgaaccg ccgttcgcgt ttttggaacg
    34201 aagcgacggc atcgtccaaa gccttgccgt caaaatcggg ccacaaaata tcggtgaaat
    34261 acagttcggc ataagccatt tgccagagta ggaaattgct gatgcgcgtt tcgccgccgg
    34321 tgcggatgaa caaatccggc tcgggcgcat cgcccagcat caagtgtttc gccagcgtgt
    34381 cttccgtaat ctcggatacg cctttggcaa tcagtttgtt tgccgcctgc aaaatgtccc
    34441 atctgccgcc gtaatcggcg gcaatgctca gggtcagacc ggtattgttt gccgtcaacg
    34501 cctccgcctc ttcgatgccc ttcaggattt gacggctgaa gcgttcgcgg ctgcccagta
    34561 ttttcaggcg catattgttt tcgtgcaggc ggcgtacctg tttttgcaaa gcctgtaaaa
    34621 acagccccat caggaacgaa acttcgtctt cggggcggcg ccagttttcg gttgaaaagg
    34681 caaacacggt cagatattgc acacccagtt tggcgcaatg cttcaccata ttttccaatg
    34741 cgtccaaacc gcgtttgtgt cccattatgc gcgggaggaa acgttttttc gcccaacggc
    34801 cgttgccgtc cataatcacg gcgatatgct tgggaatggc ggtgtgttcc aaaacggcct
    34861 gcgtgctgct tttcatgtct gcctttcgcg gttcggcatt caaatgccgt ctgaacgccg
    34921 aaccgtgcag gttaaattgc catcaaatct tcttctttgg cagtcaggag tttgtcggct
    34981 tcggtaatgt atttgtcggt cagtttttga accgcttctt cgccgcgacg tgcctcgtct
    35041 tcggaaattt ctttgtcttt gaggagtttt ttgatgtggt cgttggcatc gcggcgcacg
    35101 ttgcggatag agacgcggcc ttcttccgct tcgccgcgta cgactttaat caggtctttg
    35161 cggcgttctt cggtcagcat gggcatcggc acgcggatca ggtcgccgac agctgccggg
    35221 ttcagcccca agtttgaatc gcggatggct ttctcgactt tgaccgccat attgccctca
    35281 aacggtttca cgccgatggt gcgcgcgtcc agaagcgtta cgttggcaac ttggctgacg
    35341 gggaccatgc tgccccagta ttcgacttcc acttggtcga gcaggccggt atgcgcgcgg
    35401 ccggtacgca ctttcgccag attttctttc agtacttcga ccgaacgctg cattttgcct
    35461 tcggctgttt tttgaatatc gttgatcata ttgttctttc ggtgggataa ggtgggcggg
    35521 agaccgtctg aacgcgtttc aagccgttca gacggcataa agatcgttaa ccgcgaatag
    35581 taccgttatt cgggcataac gacaaggtag gcggattggg gatgccgtct gaagcgacag
    35641 gcgtttcaga cggcatcgtg tccgaccgtc agccgtgttc ccgtgtttca agcaggcttt
    35701 ggcgcaggtg ttggcgttcg tgggcatcca gccatttgcg gcgggtgcgt tgcagcagga
    35761 tgacgagggc ggaaatttcc tgacgcatat tggtgctgag ccagaggaag ccctgccatt
    35821 ggtagtggag gtgttcggcg agggcttcca gttcggggtt gatagcggtg tcgatgcgga
    35881 tgcggcgggc gtgtctgccg ttgataaggg cgacggtttg ttgcaggtcg gtttggagca
    35941 gtgtgaagtg gcggtcgagc agccggattt cgctgccgtt gagtttggga gattgcagct
    36001 tggcggcagt ggtcaggagc agctcggtgg tgttgacaat tttacggtgg gcgtgctgca
    36061 tggcttccat catggcgggg ctgatgcggc tttcgcccga tgtggcggcg aggtggctgc
    36121 ggcttttgac catgcgtgcg ttgatttggc gcattttcgc catgttctct tcgaggcgtt
    36181 cgcgggtcat gcgcctgccg ttgctgattt cggcaatcat tttgctgcag tcggtcaggt
    36241 tgtcggcaag catgaaacgc cacatcagtg tggatttcag cggcagcagt ttggcggcgg
    36301 cgatggcgat ggccgcgccg atgaggacgt tcatcgcgcg catcaggccg ctgtcgaacc
    36361 attcgctgcc gttgtcgccg atgagcatgc acatcgtcag ccccgccagc atagggacgt
    36421 agccgttttt gccgaccgcc gcccagccgg ccagtgcgct tgccgtgccg acggtgaggt
    36481 agaagaggag gttgccgtgg aaataatgct ggttcagcca taaaacgccc aaacccgcgc
    36541 ccagcccgat gaccgtgccg agcatacgtt ccaccgcctt ggagtaaatc gccccttgaa
    36601 actggagcat gccgaggacg acgaagacgg tcatccctat ccactcgccg tgttggaggt
    36661 ggagcagccg ggcggaggcg gtggcgaaca ggacggcccc gccgagccgg atggcgtgga
    36721 tgaggcggcg gtagcggtag cgttcgtagg agttgagcca gcggctgacg aggcggttgc
    36781 gttgcgaggt gttcatatcg gttgtgccgt ctgaagcgga aatgtgaaaa agcacaggct
    36841 tcccgaggaa gggagggtct gtgcttggta ttggtgccgg agaagggaat cgaacccccg
    36901 accttcgcgt tacgaatgcg ctgctctacc gactgagcta caccggcgtt ttttcgtcat
    36961 gatatatatg aacggttgtt tgtgcaactt ttcgggcggg cggcaaggca gtgcgcg
//
LOCUS       DAWFHY010000009        35375 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.009, whole genome shotgun sequence.
ACCESSION   DAWFHY010000009 DAWFHY010000000
VERSION     DAWFHY010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35375
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.009"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(77..1963)
                     /gene="uvrC"
                     /locus_tag="WGQ68_000391"
     CDS             complement(77..1963)
                     /gene="uvrC"
                     /locus_tag="WGQ68_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEZ0714264.1"
                     /translation="MPSETVFQVIDMNKETRFPEHFDIPLFLKNLPKLPGVYRFFDEG
                     GKVLYVGKAVNLKRRVSGYFQKNDHSPRIALMVKQVHHIETTITRSEAEALILENNLI
                     KALSPKYNILFRDDKSYPYLMLSGHQYPQMAYYRGTLKKPNQYFGPYPNSNAVRDSIQ
                     VLQKVFMLRTCEDSVFEHRDRPCLLYQIKRCTAPCVGHISEEDYRDSVRQAATFLNGK
                     TDELTRTLQHKMQTAAANLQFEEAARYRDQIQALGIMQSNQFIDSKNPNNPNDIDLLA
                     LAVSDGIVCVHWVSIRGGRHVGDKSFFPDTKNDPEPNGQDYAEAFVAQHYLGKSKPDI
                     IINNFPVPDALKEALEGEHGKQMQFVTKTIGERKVWLKMAEQNAQMAIAQRRLQQSSQ
                     QHRIDELAKILGMDSDGLNRLECFDISHTQGEATIASCVVYDEQNIQPSQYRRYNITT
                     AKPGDDYAAMREVLTRRYGKMQEAEANGETVKWPDVVLIDGGKGQIGVAVSVWEELGL
                     HIPLVGIAKGPERKAGMEELILPFTGEVFRLPPHSPALHLLQTVRDESHRFAITGHRK
                     KRDKARVTSSLGDIPGVGSKRRQALLTRFGGLRGVIAASREDLEKVEGISKALAETIY
                     NHLH"
     gene            complement(2027..4189)
                     /locus_tag="WGQ68_000392"
     CDS             complement(2027..4189)
                     /locus_tag="WGQ68_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEZ0714265.1"
                     /translation="MQQKIRFQIEGMTCQSCASRIEKVLNKKDFVESAGVNFASEEAQ
                     VTFDDSKTSAADIAKIIEKTGYGAKEKTEDTLPQPEAEHHIGWRLWLLLAINIPFLIG
                     MAGMMIGRHDWMIPPLWQFALASIVQLWLAIPFYKSAWASIKGGLANMDVLVTIGTVS
                     IYLYSVYMLFFSPHAAHGMAHVYFEVGVMVIGFVSLGKFLEHRTKKSSLNSLGLLLKL
                     TPTQVNVQRNGEWKQLPIDQVQIGDLIRANHGERIAADGIIESGSGWADESHLTGESN
                     PEEKKAGGKVLAGALMTEGSVVYRATQLGSQTLLGDMMNALSEAQGSKAPIARVADKA
                     AAVFVPTVVGIALLTFITTWLVKGDWTVALMHAVAVLVIACPCALGLATPAAIMVGMG
                     KAVKHGIWFKDAAAMEEAAHVDAVVLDKTGTLTEGRPQVAAVYCVPDSGFDEDALYRI
                     AAAVEQNAAHPLARAIVSAAQARGLGIPAAQNAQTVVGAGIAAEVESVGLVKAGKAEF
                     AELTLPKFSDGVWDIASIVAVSVDNKPIGAFALADALKADTAEAIGRLKKHNIDVYIM
                     SGDNQGTVEYVAKQLGIAHAFGNMSPRDKAAEVQKLKAAGKTVAMVGDGINDAPALAA
                     ANVSFAMKGGADVAEHTASATLMQHSVNQLADALLVSQATLKNIKQNLFFAFFYNILG
                     IPLAALGFLNPIIAGAAMAASSVSVLGNALRLKRVKID"
     gene            4328..5278
                     /gene="trxB"
                     /locus_tag="WGQ68_000393"
     CDS             4328..5278
                     /gene="trxB"
                     /locus_tag="WGQ68_000393"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEZ0714266.1"
                     /translation="MSQHRKLIILGSGPAGYTAAVYAARANLNPVIITGIAQGGQLMT
                     TTEVDNWPADADGVQGPELMARFLAHAERFGTEIIFDQINAVDLQKRPFTLKGDMGEY
                     TCDALIVATGASAKYLGLPSEEAFAGKGVSACATCDGFFYKNQDVAVVGGGNTAVEEA
                     LYLANIAKTVTLIHRRSEFRAEKIMIDKLMKRVEEGKIILKLESNLQEVLGDDRGVNG
                     ALLKNNDGSEQQIAVSGIFIAIGHKPNTDIFKGQLEMDEAGYLKTKGGTADNVGATNI
                     EGVWAAGDVKDHTYRQAITSAASGCQAALDAERWLGSQNI"
     gene            5434..5802
                     /gene="rpsF"
                     /locus_tag="WGQ68_000394"
     CDS             5434..5802
                     /gene="rpsF"
                     /locus_tag="WGQ68_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEZ0714267.1"
                     /translation="MRHYEIVFIVHPDQSEQVPAMVERYKTMIAEANGKIHRLEDWGR
                     RQLAYPINKIHKAHYVLMNIETTPEVVEELETAFRFNDAVLRHLTIKTKHAVTEASPM
                     LGGEKAKNLLSGASEEAVAQ"
     gene            5803..6105
                     /gene="priB"
                     /locus_tag="WGQ68_000395"
     CDS             5803..6105
                     /gene="priB"
                     /locus_tag="WGQ68_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HEZ0714268.1"
                     /translation="MGFNNLVSLAALIEKVFPIRYTPAGISVLDIILKHESWQKENGQ
                     QCFVQLEIPARILGRQAEEWQYRQGVYVHVEGFLAQKSRRSLMPMLRIQNIKEYKG"
     gene            6112..6342
                     /locus_tag="WGQ68_000396"
     CDS             6112..6342
                     /locus_tag="WGQ68_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEZ0714269.1"
                     /translation="MARQSFKRRKFCRFTAEKIQEVDYKQVDLLKDFISENGKIIPAR
                     ITGTKAFYQRQLAVAVKRARFLALLPYTDQHK"
     gene            6359..6811
                     /locus_tag="WGQ68_000397"
     CDS             6359..6811
                     /locus_tag="WGQ68_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEZ0714270.1"
                     /translation="MQIILLEKIGGLGNLGDIVTVKNGYARNFLIPAGKAKRATEANM
                     KEFEARRAELEAKQAEILADARARQEKLDGQTVTVAQKAGVDGRLFGSVTNADIAAAI
                     VAAGIEAVKANVRLPNGPLKAVGEYEVEVALHTDAVAKITVAVVAATE"
     gene            6825..6932
                     /locus_tag="WGQ68_000398"
     CDS             6825..6932
                     /locus_tag="WGQ68_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HEZ0714271.1"
                     /translation="MPSETLPIIGMWFQTVFVLFSVYSAKIGMVVFIVD"
     gene            7498..7653
                     /locus_tag="WGQ68_000399"
     CDS             7498..7653
                     /locus_tag="WGQ68_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714272.1"
                     /translation="MYAGHYGGNTVWEAAASRGIGTAGDAPMPSGVKAGQSAVWKKAM
                     ESVGRRS"
     gene            complement(7769..8575)
                     /locus_tag="WGQ68_000400"
     CDS             complement(7769..8575)
                     /locus_tag="WGQ68_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0714273.1"
                     /translation="MWHWDIILILLAVGSAAGFVAGLFGVGGGTLIVPVVLWVLDLQG
                     LAQHPYAQHLAVGTSFAVMVFTAFSSMLGQHKKQAVDWKTVFAMMPGMIFGVFAGALS
                     AKYIPAFGLQIFFILFLTAVAFKTLHTGPQTASRPLPKLPGLTAVSTLFGAMSSWVGI
                     GGGSLSVPFLIHCGFPAHKAIGTSSGLAWPIALSGAISYLLNGLNIAGLPEGSLGFLY
                     LPAVAVLSAATIAFAPLGVKTAHKLSSAKLKKSFGIMLLLIAGKMLYNLL"
     gene            complement(8576..9322)
                     /gene="pssA"
                     /locus_tag="WGQ68_000401"
     CDS             complement(8576..9322)
                     /gene="pssA"
                     /locus_tag="WGQ68_000401"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEZ0714274.1"
                     /translation="METPTNTPQRSLRQNSIYLLPNSFTIAALFSAFYAITQSMHGRY
                     ETAAIAVFISMLLDGMDGRVARLTNSQSAFGEQLDSLADMVSFGVAPALIAYKWQLWQ
                     FGKIGYSVAFIYCACAALRLALFNTLIGKVDKRWFIGVPSPTAAALIVGLIWVNHSVE
                     KFPAVHWWALGITLFAGLSMIVQIPFWSFKEINIRRQVPFVGMLLAVLLLLLVTWEPS
                     LVLFLFFLGYSLSGYIMAARRFWKKYRKAD"
     gene            complement(9381..9800)
                     /locus_tag="WGQ68_000402"
                     /pseudo
     CDS             complement(9381..9800)
                     /locus_tag="WGQ68_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688963.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9906..10388
                     /locus_tag="WGQ68_000403"
     CDS             9906..10388
                     /locus_tag="WGQ68_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4189 domain-containing protein"
                     /protein_id="HEZ0714275.1"
                     /translation="MMKKILAVSALCLMTAAARAADTYGYLAVWQNPQNADDVLQVKT
                     TKEDSTKSEAFAELEAFCKGQDTLAGIAEDEPTGCRSVVSLNNTCVALAYPKALGAMR
                     VENAVVITSPRFTSVHQVALNQCIKKYGVQGQCGLETVYCTSSSYYGGTVRSLIQNLK
                     "
     gene            complement(10440..11651)
                     /locus_tag="WGQ68_000404"
     CDS             complement(10440..11651)
                     /locus_tag="WGQ68_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-xanthine permease"
                     /protein_id="HEZ0714276.1"
                     /translation="MNQLKLAVSGAQILFVAFGAMVLVPLLTGLNPALALLGAGLGTL
                     LFQITTKRKVPIFLGSSFAFIAPIIYSVGEWGLPSTMFGLFAAGFMYFVFAALIRWRG
                     LAAVHKLLPPVVIGPVIMVIGLSVAVAASSMAMGQADGKQVIDYTDSLILSGFTFAVT
                     AIVSVFGSRMMKLIPILIGVASGYVLALLMGLVDTASIAHAPWFAVPHFETPQINWQA
                     ALFMLPVAVAPAIEHIGGIMAIGNVTGKDYTKDPGLDKTLAGDGLGVCVAGLIGGPPV
                     TTYGEVTGAVMITKNSNPVIMTWAAVFAVCMAFFGKFNAFLASIPMPVMGGIMLLLFG
                     TIASLGVKTLIDAKVDLMLPKNLVIVSSVLTTGIGGMTLKLGSFSFAGVGLCAVLAIM
                     LNSLLPDPKES"
     gene            11874..14312
                     /locus_tag="WGQ68_000405"
     CDS             11874..14312
                     /locus_tag="WGQ68_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEZ0714277.1"
                     /translation="MTEKSHKKTAKGRAGSPSPTSARNKKADNGARGNKVSERLKAVK
                     ELQKTETKKARPEHVVNLIGDALWLMGLAATLYLAISLISFDMGDPSWSHSSPVVEDV
                     ANWGGLFGAYVADVGYYLFGWSFWWWIAAACVVLYKNFRLHAKQTENEAYNHKIAAAA
                     LFVLTVFSPVLEYFVLGGKYADSLPVGAGGMVGIRVGAVFAWLLGKSGSLLIILVVLL
                     LSLSLLVQISWLEFLNGAGRAVQNRLSALSGKVMALGKRRPNTKTDGVDTQNTRRMVK
                     EAKNITAKPVALPEGSSSNRKSVAVSVAPPPKIQVSLFEDDEPRQAGEYHKPTLNLLR
                     IPDSEPVSINPAELERTAELIESKLAEFGIGVQVVSATSGPVITRYEIEPAQGVKGSQ
                     IVALSKDLARSMSLQSVRIVETIAGKNTMGIELPNDKRQDVMLSEILSSPVFAEAKSK
                     LTVALGKDIAGTPVVGDLAKMPHLLVAGMTGSGKSVGVNGMIMSMLFKATPDEVRFIM
                     IDPKMLELSIYDGIPHLLCPVVTDMREAGQALNWCVAEMEKRYRLLSHAGVRNLEGFN
                     QKVEAAKAAGKPLLNPFSLNPDEPEPLEKLPLIVVVIDELADLMMTERKAVEQQIARL
                     AQKARAAGIHMIVATQRPSVDVVTGLIKANIPTRMAFTVQSKIDSRTILDQMGADELL
                     KYGDSLFLQPGSAEPTRLQGAFVSDDEVHQVVNYVKSQAPADYIEGLLSGEAALETAN
                     IVNPNADSDELFDQAVAYVLESKKTSISSLQRQLRIGYNRAANLMEALENAGVVSPAD
                     LNGSRKILAHKDHL"
     gene            14521..15831
                     /locus_tag="WGQ68_000406"
     CDS             14521..15831
                     /locus_tag="WGQ68_000406"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003742150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEZ0714278.1"
                     /translation="MSVTVETLENLERKVVLSLPWSEINAETDKKLKQTQRRAKIDGF
                     RPGKVPLKMIAQMYGASAQNDVINELVQRRFHDVAVAQELKVAGFPRFEGVEEQDDKE
                     SFKVAAIFEVFPEVVIGDLSAQEVEKVTASVGDAEVDQTVEILRKQRTRFNHVEREAR
                     NGDRVIIDFEGKIDGEPFAGGASKNYAFVLGASQMLPEFEAGVVGMKAGESKDVTVNF
                     PEDYHGKDVAGKTAVFTITLNNVSEATLPEVDADFAKALGIADGDVAKMREEVQKNVS
                     REVERRVNEQTKESVMNALLKAVELKAPVALVNEEAARLANEMKQNFVNQGMADAANL
                     DLPLDMFKEQAERRVSLGLILAKLVDENKLEPTEEQIKAVVANFAESYEDPQEVIDWY
                     YADPSRLQAPTSLAVESNVVDFVLGKAKVNEKALSFDEVMGAQA"
     gene            15927..16541
                     /gene="clpP"
                     /locus_tag="WGQ68_000407"
     CDS             15927..16541
                     /gene="clpP"
                     /locus_tag="WGQ68_000407"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEZ0714279.1"
                     /translation="MSFDNYLVPTVIEQSGRGERAFDIYSRLLKERIVFLVGPVTDES
                     ANLVVAQLLFLESENPDKDIFFYINSPGGSVTAGMSIYDTMNFIKPDVSTLCLGQAAS
                     MGAFLLSAGEKGKRFALPNSRIMIHQPLISGGLGGQASDIEIHARELLKIKEKLNRLM
                     AKHCGRDLADLERDTDRDNFMSAEEAKEYGLIDQILENRASLQF"
     gene            complement(16597..17862)
                     /gene="ispG"
                     /locus_tag="WGQ68_000408"
                     /gene_synonym="gcpE"
     CDS             complement(16597..17862)
                     /gene="ispG"
                     /locus_tag="WGQ68_000408"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEZ0714280.1"
                     /translation="MNTLQRRKTHQVRIDHITVGSEAPVVIQSMTNTDTADAKATALQ
                     IKELSDAGSEMVRITVNSPEAASKVAEIRRRLDDMGYATPLIGDFHFNGERLLAEFPE
                     CGKALSKYRINPGNVGKGVKGDEKFAFMIRTAAENDKAVRIGVNWGSLDQSLAKRMMD
                     ANLASSAPKPPEEVTKEALIVSALESAEKAVLLGLPEDKIILSCKVSAVQDLIQVYRE
                     LGSRCAYPLHLGLTEAGMGSKGIVASTAALSVLLQEGIGDTIRISLTPEPGSPRTQEV
                     VVGQEILQTMGLRSFTPMVTACPGCGRTTSTVFQELAQDVQNYLRQKMSIWRTLYPGV
                     ESLNVAVMGCVVNGPGESKLADIGISLPGTGETPVAPVYVDGERKVTLKGDNIATEFL
                     AIVEEYVKTNYGENGSKRNQNKIIPIQSL"
     gene            complement(17878..18639)
                     /gene="pilW"
                     /locus_tag="WGQ68_000409"
     CDS             complement(17878..18639)
                     /gene="pilW"
                     /locus_tag="WGQ68_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HEZ0714281.1"
                     /translation="MPFKPSKRISLLLVLALGACSTSYRPSRAEKANQVSNIKTQLAM
                     EYMRGQDYRQATASIEDALKSDPKNELAWLVRAEIYQYLKVNDKAQESFRQALSIKPD
                     SAEINNNYGWFLCGRLNRPAESMAYFDKALADPTYPTPYIANLNKGICSAKQGQFGLA
                     EAYLKRSLAAQPQFPPAFKELARTKMLAGQLGDADYYFKKYQSRVEVLQADDLLLGWK
                     IAKALGNAQAAYEYEAQLQANFPYSEELQTVLTGQ"
     gene            complement(18642..19736)
                     /gene="rlmN"
                     /locus_tag="WGQ68_000410"
     CDS             complement(18642..19736)
                     /gene="rlmN"
                     /locus_tag="WGQ68_000410"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEZ0714282.1"
                     /translation="MKTNLLNYDLQGLTRHFADTGEKPFRAKQVMRWMHQSGAQNFNE
                     MTDLAKSLRHKLNEQASIEIPKLMMSQESSDGTRKWLLDVGTGNGVETVFIPESDRGT
                     LCISSQVGCALECTFCSTGRQGFNRNLTAAEIIGQLWWANKAMGVTPKNERVISNVVM
                     MGMGEPMANFDNVVTALSIMLDDHGYGLSRRRVTVSTSGMVPQMDRLRDVMPVALAVS
                     LHASNDEVRNQIVPLNKKYPLKELMAACQRYLVKAPRDFITFEYVMLDGINDRAQHAR
                     ELIELVKDVPCKFNLIPFNPFPNSGYERSSNENIRVFRDILQQAGFVVTVRKTRGDDI
                     DAACGQLAGQVQDKTRRQQKWQQILIGQQG"
     gene            complement(19879..20304)
                     /gene="ndk"
                     /locus_tag="WGQ68_000411"
     CDS             complement(19879..20304)
                     /gene="ndk"
                     /locus_tag="WGQ68_000411"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEZ0714283.1"
                     /translation="MAIERTISIIKPDAVGKNVIGKIYSRFEENGLKIVAAKMKQLTL
                     KEAQEFYAVHKDRPFYAGLVEFMTGGPVMIQVLEGENAVLKNRELMGATNPSEAAEGT
                     IRADFATSVSINAVHGSDSVENAALEIAYFFSQTEICPR"
     gene            complement(20363..21514)
                     /locus_tag="WGQ68_000412"
     CDS             complement(20363..21514)
                     /locus_tag="WGQ68_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1 family ATPase"
                     /protein_id="HEZ0714284.1"
                     /translation="MSNRDQLFKAPPFENHSPLTWYQAASQLPNFIRDDAQAAAIEHL
                     DRLWTELMMFKRKRNRFLGRSLRSPQVPKGLYFYGGVGRGKSFLMDAFFGCLPYRRKR
                     RVHFHAFMAEIHQRLKTLKSESNPLKSVAAEIAKETRVLCFDEFHVSDIADAMILGRL
                     LENLLNEGVVLVATSNYAPSELYPQGQNRSSFLPTIALIESSLTVLNVDGGEDYRLRT
                     LRPAEIFFTPANEENEAKLAKLFKEMTGITDLNPGISTIHGREIPHKAESGRAIWFDF
                     RALCFGPRSQSDYLYLAEHYEMVFISGLEQLSPQEKAEARRLTWLIDVLYDFRVKLCA
                     TGAVDVNHIYTEGDFAEEFTRTASRMVEMQSEVYLEQPHLTLSPKASGG"
     gene            complement(22050..22877)
                     /gene="fghA"
                     /locus_tag="WGQ68_000413"
     CDS             complement(22050..22877)
                     /gene="fghA"
                     /locus_tag="WGQ68_000413"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011961786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEZ0714285.1"
                     /translation="MKLIEQHQIFGGSQQVWAHHAQTLQCEMKFAVYLPNNPENRPLG
                     VIYWLSGLTCTEQNFITKSGFQRYAAEHQVIVVASDTSPRGEQVPNDDAYDLGQGAGF
                     YLNATEQPWAANYQMYDYILNELPRLIEKHFPTNGKRSIMGHSMGGHGALVLALRNRE
                     HYQSVSAFSPILSPSLVPWGEKAFTAYLGKDREKWQQYDANSLIQQGYKVQGMRIDQG
                     LEDEFLPTQLRTEDFIETCRAANQPVDVRFHKGYDHSYYFIASFIGEHIAYHAAFLK"
     gene            complement(22886..23995)
                     /locus_tag="WGQ68_000414"
     CDS             complement(22886..23995)
                     /locus_tag="WGQ68_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEZ0714286.1"
                     /translation="MKSRAAVAFAPNQPLQIVEIDVEMPRKGEVLIRNTHTGVCHTDA
                     FTLSGSDPEGVFPVVLGHEGAGVVVAVGEGVSSVKPGDHVIPLYTAECGECEFCCSGK
                     TNLCVSVRDTQGKGLMPDGTTRFSYQGQPIYHYMGCSTFSEYSVVAEVSLAKINPEAN
                     HEQVCLLGCGVTTGIGAVHNTAKVQEGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPAKFELAKQFGATDCLNPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRQALES
                     AHRGWGQSIIIGVAGAGQEISTRPFQLVTGRVWKGSAFGGVKGRSELPKMVEDSMKGD
                     IELEPFVTHTMTLDQINKAFELMHEGKSIRAVIHY"
     gene            24143..24550
                     /locus_tag="WGQ68_000415"
     CDS             24143..24550
                     /locus_tag="WGQ68_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEZ0714287.1"
                     /translation="MTYTTAKAAEKIGISAHTLRFYDKEGLLPNIGRDEYGNRCFTDN
                     DLQWLGLLQCLKNTGMSLKDIKRFAECTVIGDDTIEERLSLFENQIENVKCQIAELKR
                     YLDLLEYKLAFYQKAKALGSVKAVNLPQIPETA"
     gene            complement(24901..25215)
                     /locus_tag="WGQ68_000416"
     CDS             complement(24901..25215)
                     /locus_tag="WGQ68_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEZ0714288.1"
                     /translation="MTKSELMVRLAEVFAAKNGTHLLAKDVEYSVKVLVDTMTRSLAR
                     GQRIEIRGFGSFDLNHRPARIGRNPKTGERVEVPEKHVPHFKPGKELRERVDLALKEN
                     AN"
     gene            complement(25226..26911)
                     /gene="rpsA"
                     /locus_tag="WGQ68_000417"
     CDS             complement(25226..26911)
                     /gene="rpsA"
                     /locus_tag="WGQ68_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEZ0714289.1"
                     /translation="MSMENFAQLLEESFTLQEMNPGEVITAEVVAIDQNFVTVNAGLK
                     SESLIDVAEFKNAQGEIEVKVGDFVTVTIESVENGFGETKLSREKAKRAADWIALEEA
                     MENGNILSGIINGKVKGGLTVMISSIRAFLPGSLVDVRPVKDTSHFEGKEIEFKVIKL
                     DKKRNNVVVSRRAVLEATLGEERKALLENLQEGSVIKGIVKNITDYGAFVDLGGIDGL
                     LHITDLAWRRVKHPSEVLEVGQEVEAKVLKFDQEKQRVSLGMKQLGEDPWSGLTRRYP
                     QGTRLFGKVSNLTDYGAFVEIEQGIEGLVHVSEMDWTNKNVHPSKVVQLGDEVEVMIL
                     EIDEGRRRISLGMKQCQANPWEEFAANHNKGDKISGAVKSITDFGVFVGLPGGIDGLV
                     HLSDLSWTESGEEAVRKYKKGEEVEAVVLAIDVEKERISLGIKQLEGDPFGNFISVND
                     KGSLVKGSVKSVDAKGAVIALSDEVEGYLPASEFAADRVEDLTTKLKEGDEVEAVIVT
                     VDRKNRSIKLSVKAKDAKESREALNSVNAAANANAGTTSLGDLLKAKLSGEQE"
     gene            complement(27066..27722)
                     /locus_tag="WGQ68_000418"
     CDS             complement(27066..27722)
                     /locus_tag="WGQ68_000418"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEZ0714290.1"
                     /translation="MNRQKVIAIDGPGASGKGTVAARVAAALGYDYLDTGALYRLTAL
                     YAQKQGVEWHDEENVSKLAKKLPAVFSGSRILLDGEDVSDGIRTEAIGMGASAVAQLP
                     KVRAALLQRQRDFLTEKGLVADGRDTGSVVFPQAELKIFLTAESKIRAERRAKQIGIP
                     CEGLAFERILSDIEARDEADRNRKVAPLKQQPDALLLDTSRLTIEETVKKVLDWYRKV
                     "
     gene            complement(27966..28774)
                     /locus_tag="WGQ68_000419"
                     /pseudo
     CDS             complement(27966..28774)
                     /locus_tag="WGQ68_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232575.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            complement(28834..29276)
                     /locus_tag="WGQ68_000420"
                     /pseudo
     CDS             complement(28834..29276)
                     /locus_tag="WGQ68_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
     gene            29602..29708
                     /locus_tag="WGQ68_000421"
                     /pseudo
     CDS             29602..29708
                     /locus_tag="WGQ68_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(29910..30602)
                     /locus_tag="WGQ68_000422"
     CDS             complement(29910..30602)
                     /locus_tag="WGQ68_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEZ0714291.1"
                     /translation="MKLIKYLQYQGIGSRKQCQWLIAGGYVFINGTCMDDTDADIDSS
                     SVETLDIDGEAVTVVPEPYFYIMLNKPEDYETSHKPKHYRSVFSLFPDNMRNIDMQAV
                     GRLDADTTGVLLITNDGKLNHNLTSPSRKIPKLYEVTLKHPTGETLCETLKNGVLLHD
                     ENETVCAADAVLKNPTTLLLTITEGKYHQVKRMIAAAGNRVQHLHRRRFAHLETENLK
                     PGEWKFIECPKF"
     gene            complement(30670..32646)
                     /locus_tag="WGQ68_000423"
     CDS             complement(30670..32646)
                     /locus_tag="WGQ68_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0714292.1"
                     /translation="MSKLKTIALTASGLSVCPGFLYAQNTSSHQIGLAIMRLNSSILD
                     LPPTKQYFQSGSLWSELRQGFRMGEVNPELVRRHESKFIASHSYFNRVINRSRPYMYH
                     IANEVKKRNMPAEAALLPFIESAFVTKAKSHVGASGLWQFMPATGRHYGLEKTPVYDG
                     RHDVYAATDAALNYLQYLYGLFGDWPLAFAAYNWGEGNVGRAINRARAQGLEPTYENL
                     RMPNETRNYVPKLLAVRNIIATPQSFGMNISDIDNKPYFQAVEPDRPLDNEAIARLAG
                     ITQSELLALNPAFNVPAFIPKSKRKLLLPVASVQTFQSNYLNAAPDSLFSWEVYTPAA
                     KTSLSDISTATGMSIADIKRLNNLNGNLVNAGRSILVAKNGKTLQTASESVVSIDIDN
                     TPDTYRSNMPAGTVNVGIARIRPAAAQTADITVAPLPQKTVRTEPDPLVRIAEPALAT
                     AAAQPQTEKQTAMPSETQTATLAQIVPQNDMQAADELMQLVARNNLRRQAEETISAVI
                     GTPDTVAEHNISSSPQHTVAADGKRRVRLETRVAKAADGEAEISPLHASIHRVVEGDT
                     LFNIAKRYNVSVADLIVANNIKGNTIQKGQVLRLAQAAPAQTRIEKVSYTARKGDTFK
                     SIAARFNIHIDDIRRLNPNLNTINPGQRVKLIGS"
     gene            32825..33490
                     /locus_tag="WGQ68_000424"
     CDS             32825..33490
                     /locus_tag="WGQ68_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEZ0714293.1"
                     /translation="MDAWFEDTAMGRYVAKLEQDFFGRYLDSYRFSGMCAVQVGGRWL
                     SLSEDVVCVPRDMSMSAENMALADVSADMLLFPHTLEGGVPSQILSEAHRILKPSGRL
                     MLTGFNPYSLWGFSCWFDGERLPEKRFCLPLPELKRRLADVGFDIEFGKFMVYLPPVS
                     SLGQIRFWRFMEKAGDRWWPQCAAVYGLVLVKRAAGVTPLPAWDGYLGGKALAAGAAR
                     VAD"
     gene            33542..34369
                     /gene="mutM"
                     /locus_tag="WGQ68_000425"
     CDS             33542..34369
                     /gene="mutM"
                     /locus_tag="WGQ68_000425"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEZ0714294.1"
                     /translation="MPELPEVETTLRGIAPHIEGKTVEAVVLRQLKLRWQINPDLGEI
                     LSGRQVLSCGRRAKYLLIRFQTGVLLIHLGMSGSLRIFTPSDGRIGRPDRHDHVDIVF
                     SDGTVMRYRDPRKFGAILWYEGIEEHHPLLEKLGPEPLSEAFCTDYLYVRLKAQKRAV
                     KLALMDNAVVVGVGNIYANESLFRAGISPHRPANRLKKKECALLVETVKAVLRRAIET
                     GGSTLRDFMDSDGKSGYFQQEYTVYGRHNQPCPQCGGLVVKETLGQRGTFYCPNCQK"
     gene            34457..35224
                     /locus_tag="WGQ68_000426"
     CDS             34457..35224
                     /locus_tag="WGQ68_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0714295.1"
                     /translation="MSSNKASFFTRLRRLCRLTVWLFKTGKNLRGIDGGCPESRNRAV
                     IELGRGALTALDIGLEVGRPAPEHPNGVLVAANHVSWLDIFAMSAVYPSSFIAKQEIK
                     SWPVLGKMGQNAGTVFINRNSRRDIEPINRAVCETLQRGQNVSFFPEARTSSGLGLLP
                     FKAALFQSAIDAGAKVLAVALRYYDETGKRTARPSYADVGLPTCLWRIVSMKKLTIKV
                     DFVCVADAAESEDRYALKDKIEESIRAVVADDADIAV"
BASE COUNT         8412 a         8473 c         9638 g         8852 t
ORIGIN      
        1 aatttaatcc actatatatc gatataccgg cctgctgtat catattttta cagacggttt
       61 ttgttttggc ggcatactaa tgcagatgat tataaatggt ttccgccaat gccttgctga
      121 tgccttccac tttttccaag tcctcgcggc tggcggcaat cacgccgcgc agaccgccga
      181 agcgggtaag cagggcttgg cggcgtttgc tgcctacgcc gggaatgtcg ccgagggatg
      241 aggtaacgcg cgctttgtcg cgttttttgc ggtggcctgt gatggcaaag cggtgcgatt
      301 catcgcgtac ggtttgcaat agatgcaagg ccgggctgtg tgggggcagg cggaagactt
      361 cgccggtaaa aggcagtatg agttcttcca taccggcttt gcgctccggg cctttggcga
      421 taccgaccaa aggaatgtgc agcccgagtt cttcccatac cgatacggct acgccgattt
      481 gccctttgcc gccgtcaatc aatacgacat cgggccattt gacggtctcg ccgttggctt
      541 cggcctcctg cattttgccg taacggcgcg tcaacacttc gcgcatggcg gcgtagtcgt
      601 cgccgggttt ggcggtcgtg atgttgtagc ggcggtattg cgagggctgg atgttttgct
      661 catcgtacac aacgcaggac gcaatagtgg cctcgccttg ggtgtggctg atgtcgaaac
      721 attcgaggcg gttgaggccg tctgaatcca taccgaggat tttggcgagt tcgtcgatgc
      781 ggtgttgctg gctgctttgt tgcaggcggc gttgtgcaat cgccatttgt gcgttttgct
      841 ccgccatttt caaccagact ttgcgttcgc ctatggtttt ggttacgaac tgcatttgtt
      901 tgccgtgttc gccttccaaa gcctctttca gcgcatcggg gacggggaag ttgttgatga
      961 taatgtcggg tttgcttttg cccagatagt gttgggcgac gaaggcttcg gcatagtctt
     1021 gcccgtttgg ctcgggatcg tttttggtgt cggggaagaa acttttgtct ccgacgtgcc
     1081 gcccaccgcg tatgctgacc cagtgtacgc agacgatgcc gtctgaaacc gccagcgcga
     1141 gcaaatcgat atcgttgggg ttgttcgggt ttttgctgtc gatgaactgg ttgctctgca
     1201 taatgccgag tgcttggatt tggtcgcggt agcgggcggc ttcttcaaat tgcagattcg
     1261 cggcggcagt ttgcattttg tgttgcaggg tgcgggtcag ttcgtcggtt ttgccgttga
     1321 gaaaagtggc ggcttgacgc acgctgtcgc ggtagtcttc ttcgctgatg tgtccgacgc
     1381 agggcgcggt gcagcgtttg atttgataca gcaggcaagg gcggtcacga tgctcgaata
     1441 cgctgtcttc gcaggtacgc agcataaaga ctttttgcaa cacttgaatg ctgtcgcgca
     1501 cggcgttgct gttgggatac gggccgaagt attggtttgg ctttttcagc gtgccgcggt
     1561 aatacgccat ctgcggatat tgatggccgc tgagcatcaa ataaggatag cttttgtcgt
     1621 cgcggaaaag gatattgtat ttcggcgaca aggctttgat gaggttgttt tcgagaatca
     1681 gggcttcggc ttcggaacgc gtgatggtgg tttcgatatg atgaacctgt tttaccatca
     1741 atgcgatgcg cggcgaatgg tcgttttttt ggaaatagcc ggacacgcgc cgcttgaggt
     1801 tgaccgcttt gccgacgtat aagactttgc cgccttcgtc aaaaaaacgg tacacgcccg
     1861 gcagcttggg caggtttttg aggaaaagtg ggatgtcgaa gtgttccgga aaacgggttt
     1921 ctttattcat atcaattact tgaaaaacgg tttcagacgg catattacca tatcgctgga
     1981 gacagcctta tccgttcgga caaggctgca gggcggttac atgctgctaa tcgattttca
     2041 cccgtttcag gcgcaaggca ttgcccaaca cggaaaccga gcttgccgcc atggccgcgc
     2101 ctgcgatgat gggatttaaa aagccaagcg cggcgagcgg aatgcccaag atattgtaga
     2161 agaaagcgaa aaacaggttt tgcttgatgt ttttcaaagt ggcttgcgac accagcaggg
     2221 catcggcgag ctgattaacc gaatgctgca tcagcgtggc ggacgcggtg tgttcggcaa
     2281 cgtccgcccc gcctttcatc gcgaagctaa cattggcggc ggcgagcgcg ggcgcgtcgt
     2341 tgatgccgtc gccgaccatc gccacggttt tgccggcggc tttgagtttt tgcacttcgg
     2401 cggctttgtc gcgcggactc atattaccga aggcgtgtgc gatgcccagt tgtttggcga
     2461 cgtattcgac cgtgccttgg ttgtcgccgc tcataatata gacatcgata ttgtgttttt
     2521 tcagacggcc tatggcttcg gcggtatcgg ctttcaacgc gtcggcaagt gcgaatgcgc
     2581 cgatgggttt gttatcgacc gagactgcaa caatgcttgc aatgtcccaa acgccgtctg
     2641 aaaacttcgg caaggtcagt tcggcaaatt cggctttgcc cgctttcacc aaccccacgc
     2701 tttccacttc ggcggcaatg cctgcgccga caacggtttg cgcgttttgt gcggcgggaa
     2761 tccccaagcc gcgcgcttgg gcggcggaaa cgatggcgcg ggcaagcgga tgagcggcgt
     2821 tttgttcgac ggcggcggcg atgcggtaca aagcgtcttc gtcaaagccg ctgtcgggaa
     2881 cgcaatagac ggcggcaacc tgcggcctgc cttcggtcag cgtgccggtt ttgtccaaca
     2941 cgacggcatc gacgtgggcg gcttcttcca ttgccgccgc gtctttaaac caaatgccgt
     3001 gtttcactgc tttacccatg ccgaccataa tcgcggcagg ggtcgccaga ccgagcgcac
     3061 atgggcaggc aatcaccaaa acggcaacgg catgcatcag cgcaaccgtc caatcgcctt
     3121 tcaccagcca agtggtgata aaagtcaaaa gcgcaatgcc cacaacagtt ggcacaaaca
     3181 ccgccgccgc tttatcggct acgcgcgcaa tcggtgcttt actgccttgc gcttcggata
     3241 gcgcgttcat catgtcgcca agcagggttt ggctgccgag ctgcgtggcg cggtacacca
     3301 cgctgccttc ggtcatcagc gcgcccgcca acactttgcc tcctgccttt ttctcttcgg
     3361 gattggattc gccggtgagg tggctctcgt ccgcccagcc gctgccgctt tcaatgatgc
     3421 cgtcggcggc aatgcgttca ccgtggttgg cgcggattag atcgccgatt tgcacttggt
     3481 cgatgggaag ctgtttccat tcgccgttgc gttgtacatt gacttgggtc ggcgtgagtt
     3541 tgagcagcaa acccaagctg ttcaggctgg attttttggt gcggtgttcc aaaaatttac
     3601 ccagtgacac aaaaccaatc accattacgc ccacttcaaa atacacatgc gccataccgt
     3661 gcgccgcgtg cgggctgaaa aacagcatat agacggaata caggtaaatc gacaccgtac
     3721 cgatggtaac gagcacgtcc atattcgcca gtccgccctt aatgctcgcc cacgcgcttt
     3781 tgtaaaacgg gatggccagc caaagctgca caatgcttgc caatgcaaac tgccacaacg
     3841 gcggaatcat ccaatcgtgc cgcccaatca tcatgcctgc catgccgatt aagaacggga
     3901 tgttgatggc cagcaaaagc cacagcctcc agccgatatg gtgttccgct tcaggttgcg
     3961 gcaatgtatc ttccgtcttt tccttcgcac cgtaaccggt tttctcaatg attttggcaa
     4021 tgtcggcagc ggaagttttg ctgtcgtcaa acgtaacctg cgcctcctcg ctggcaaagt
     4081 tcacccctgc cgattcgaca aaatcttttt tgttcaacac tttttcaata cgcgaagcac
     4141 aggactgaca agtcatgcct tcgatttgga aacggatttt ttgttgcata agtttgattc
     4201 ctgctttgat gttcagacgg cagtcaaggt tgcgggatgc cgtctgaacg cccgtatggt
     4261 caaaatttct caaacgcgcc gataattcga gtagaatccg aaaacttgac acagcaaagg
     4321 aagaacaatg tcccaacacc gcaaactgat tattttgggt tccggtcccg ccggatacac
     4381 cgccgccgtc tatgccgcgc gcgccaattt aaaccccgtc attattacag gtatcgcgca
     4441 aggcgggcaa ctgatgacca ccaccgaagt ggacaactgg cctgccgatg ccgacggcgt
     4501 gcaagggccg gaattgatgg cgcggtttct cgcccacgcc gaacgtttcg gaacggaaat
     4561 catttttgac caaatcaacg ccgtcgatct gcaaaaacgc ccgtttacac tcaaaggcga
     4621 tatgggcgag tacacttgcg atgccctgat tgtcgcaaca ggcgcgtccg ccaaatacct
     4681 cggtttgccg agtgaggaag cgtttgccgg aaaaggcgtt tccgcttgtg ccacctgcga
     4741 cggtttcttc tacaaaaacc aagatgttgc cgtagtcggc ggcggcaata cggcagttga
     4801 agaggcactc taccttgcca atatcgccaa aaccgttacc ctgatccacc gccgcagcga
     4861 gttccgtgcc gaaaaaatca tgatcgacaa actgatgaaa cgcgtggaag agggcaaaat
     4921 catcctcaag ctggaaagca acctgcaaga agtactgggc gacgatcggg gcgtaaacgg
     4981 cgcattatta aaaaacaacg acggttctga gcaacaaatt gccgtcagcg gcatttttat
     5041 cgccatcggg cacaagccga ataccgatat tttcaaaggt cagttggaaa tggacgaagc
     5101 cggttacctg aaaaccaaag gcggcacggc ggacaatgtc ggcgcaacca atatcgaagg
     5161 cgtatgggcg gcgggcgacg taaaagacca tacctaccgt caggcgatta ccagcgcggc
     5221 ttccggctgt caggcggctt tggacgcgga acgctggctg ggcagccaaa acatttgaat
     5281 gccgtctgaa accgaaattt tcggcggaat gcaagttttt cgcaccaaat ccttgattcc
     5341 gtcggcaaac cccaatataa tgcctgctct ttgaaccttg ctttctgctt tcgcatggca
     5401 gaaggctgtt tttaccatcc ataaggagat aacatgcgtc attacgagat cgtgtttatc
     5461 gttcatcctg atcaaagcga gcaagtgcct gctatggttg aacgttacaa aaccatgatt
     5521 gccgaagcaa acggtaagat tcaccgtctg gaagattggg gtcgccgcca actggcttac
     5581 ccgattaaca aaatccataa agcccattac gttttgatga acatcgaaac cactcccgaa
     5641 gtggtcgaag agttggaaac cgcgttccgt ttcaatgatg cggtattgcg tcatctgacc
     5701 atcaaaacca aacacgccgt taccgaagca tcccctatgt tgggtggcga aaaggctaag
     5761 aacctgctga gcggtgcgtc tgaagaagcg gttgcccaat aattgggatt caataatctt
     5821 gtttcgcttg ccgcgttaat tgaaaaggtt ttccctattc gatatacgcc tgccggaatc
     5881 tctgtcttag atattatctt aaagcatgaa tcatggcaga aagaaaatgg acagcaatgt
     5941 tttgtccaat tggaaattcc ggcacggatt ttaggcaggc aggcggaaga gtggcagtat
     6001 cggcaaggtg tatatgttca cgtcgaaggt tttttggctc aaaaaagcag acgttccctg
     6061 atgccgatgc tcagaataca aaatattaaa gaatataaag gttaaacgac aatggctcgt
     6121 caatcattca aacgtagaaa attctgccgt ttcacggctg aaaaaatcca agaagtcgat
     6181 tacaaacaag ttgatttgct gaaagacttt atctctgaaa acggtaaaat cattcctgcc
     6241 cgcatcacag gaacgaaggc attttaccaa cgccaattgg ctgttgccgt aaaacgcgcg
     6301 cgcttcttgg ctctgctgcc ttacaccgac caacacaaat aattttggag attaaatcat
     6361 gcaaattatt ctgttagaaa aaatcggcgg tctgggcaac ttgggcgaca tcgtaaccgt
     6421 taaaaacggt tatgcccgca acttcttgat tcccgcaggt aaggcaaaac gtgctactga
     6481 agcgaacatg aaagagtttg aagcacgccg tgccgaattg gaagccaaac aggccgaaat
     6541 tttggcagat gcccgagccc gtcaggaaaa attggacggt caaaccgtta ccgttgcaca
     6601 aaaagccggt gtggacggcc gcctgttcgg ttccgttacc aatgccgaca ttgctgctgc
     6661 aatcgttgct gccggcatcg aagccgtgaa agcaaatgta cgtctgccga acggtcctct
     6721 gaaagccgtt ggcgagtacg aagtggaagt ggctttgcac actgatgccg ttgctaaaat
     6781 taccgttgcc gtcgttgccg caaccgagta attgaaatta tcagatgccg tctgaaaccc
     6841 tgccgattat cggaatgtgg tttcagacgg tatttgtttt gttttccgta tattctgcaa
     6901 aaatcgggat ggttgttttt atagtggatt aaatttaaac cagtacagcg ttgcctcgcc
     6961 ttgccgtgct atctgtactg tctgcggctt cgtcgccttg tcctgatttt tgttaatcca
     7021 ctatatttgc ctgtggtctt tgcaggaata gtttgttaac gaaatttgat gcataaaatg
     7081 cgccaaaaaa tattggctgt gttttaatat agtgttgata agcgtacctg gcttcaatat
     7141 actcaatagg ataggtctct tcctaaccga aaaaataatt gtcttttccc ttgcaaataa
     7201 ttgaaatcaa cagattacta aaacacagcc aaaatattca attgacaaaa acttttctga
     7261 ttttgagaaa tctgtgaaaa tcaggggatt atttattttg aaaatggtat cgggcataaa
     7321 atttggctgt gtttcaatat ggtgctgata acatactttg tttcaaaatg ttcaatagga
     7381 taacttcctg attgaccgaa aaataatggc cttttccctt gcaagcagtt gaaatcaaca
     7441 gattgtcaaa atacatccta aaaatttaat aacttatatg attgcaaagg ttgcggcttg
     7501 tatgccggtc attatggcgg aaataccgtt tgggaggcag ccgcatcgcg tggaatcggc
     7561 acggcaggcg atgcgccgat gccttcgggc gtgaaggcgg ggcaatcggc agtttggaaa
     7621 aaggcaatgg aatctgtggg cagaagaagt taagggtaaa agtcgggcgc acggccaggt
     7681 tgtctgtcct tttgtcggaa caggagactg ggcagctttg tcctgattaa ttgcttgtgc
     7741 aaacggtaaa aagggttttt cgtgtgtttt aaagcaggtt gtacagcatt tttccggcaa
     7801 tcaaaagcag cataatgccg aaggattttt tgagtttggc agaagaaagt ttgtgggcgg
     7861 ttttgacacc gagcggggca aaggcaatgg ttgccgcgct gaggacggcg acggcgggca
     7921 ggtaaaggaa gcccagcgac ccttcgggca atcctgcaat attcaggccg ttgagcagat
     7981 acgatattgc gccggagagt gcaatcggcc aggcaaggcc ggatgatgtg ccgatggctt
     8041 tatgggcggg gaagccgcag tggattaaga aggggacgga aagtgaaccg ccgcctatgc
     8101 cgacccagct cgacattgcg ccgaacagtg tggaaaccgc agtcagtccg ggcagcttgg
     8161 gcagcgggcg ggatgccgtc tgagggccag tatgcagtgt tttgaatgcg acggcagtta
     8221 aaaacaggat gaagaaaatt tgaagcccga atgcggggat atattttgcg gagagtgcgc
     8281 ccgcgaatac gccgaatatc atacccggca tcatcgcaaa tacggttttc cagtcgaccg
     8341 cctgtttttt gtgctgcccc aacatactgg aaaaggcggt gaagaccatg acggcgaagg
     8401 atgtgccgac ggcgaggtgt tgcgcgtaag gatgttgtgc caaaccctgc aaatcaagca
     8461 cccataaaac gacagggaca atcagcgtgc cgccgcctac gccgaacagg ccggcgacaa
     8521 aacctgccgc actgcctacg gcaagcagga ttaagataat gtcccaatgc cacatttaat
     8581 ccgcctttct gtattttttc cagaatcggc gtgccgccat aatgtagccg gacaggctgt
     8641 atccgagaaa gaacaggaag aggacgagcg acggttccca agtgaccaga agcagcagta
     8701 agacggcaag cagcattccg acaaagggga cttgtctgcg gatgttgatt tctttaaaac
     8761 tccaaaaagg gatttggaca atcatcgaca gaccggcaaa cagtgtgatg cccaatgccc
     8821 accagtggac ggcggggaat ttttcgacgc tgtggttgac ccaaatcagc ccgacaatca
     8881 gcgcggcagc agtcggactg ggcacgccga taaaccagcg tttgtccacc ttgccgatga
     8941 gcgtattgaa cagcgcaagg cgcagggcgg cgcaggcgca gtagatgaag gcgacggaat
     9001 aaccgatttt gccgaactgc caaagttgcc atttgtaggc aataagcgcg ggggcaacgc
     9061 cgaagctgac catgtcggca aggctgtcga gctgttcccc gaacgcgctt tggctgttgg
     9121 tcagccgcgc cacgcgcccg tccataccgt ccaaaagcat ggaaatgaat acggcaatag
     9181 cggcggtttc ataacgtccg tgcatggatt gggtgattgc gtaaaacgcg gaaaacagcg
     9241 cggcgatagt aaaggagttg ggcagcaggt agatactgtt ttgacgcagg gagcgttgcg
     9301 gggtgttggt cggggtttcc atagctgaag tgtctcacgg acgtggagaa tcgatagggg
     9361 attataaccc aaaccattat tcgaacaata cgccctttag gatgaagccc tcggaggcgg
     9421 tttgtttttc tgatggcgga tgctttgcgg ggcggtttga ctctgcctgc caccgggtgt
     9481 gttgtttcgg atttttgccg gatggatatt gttgccgggc atttctgggc ttgccggaag
     9541 gtatcggggg gagggcatcg gaatatgcgg cgcaccgttc aaaccggggc ggcgtttccc
     9601 tattttcagg gggttgcagg tttcaacaaa ttgaaagata ttttttgcat ttccgaccgt
     9661 atgctgattt tgaagcggga taaattatag gacaaggttt ttcgcccgcg gcaaggattg
     9721 cccgcaatgc cgtctgaaaa caacggcttg ccgctaaggc ggcggtatgc ggatttgcgc
     9781 tgccgaaatc ggtttgccat acccggcggt gcaatctgcg gggagggata agccgataag
     9841 gcgggactta tcctgttttt tgtttataat gcaacagaat ttggacgaac cgtcgggaat
     9901 ttatcatgat gaaaaagata ctggccgtat cggcactatg cctgatgact gcggcggcac
     9961 gggctgccga tacttacggc tatctcgccg tttggcagaa tccgcagaat gcggacgatg
    10021 ttttgcaggt taaaaccaca aaagaagatt cgacgaaaag cgaagcgttt gccgagttgg
    10081 aagccttctg caaaggtcag gacacgcttg cgggcattgc cgaagacgag ccgaccggat
    10141 gccggtcggt cgtgtcgctg aacaatacct gtgtcgcgct ggcatacccg aaagccttgg
    10201 gcgcgatgcg cgttgaaaac gccgtcgtga ttacttctcc gcgttttacg agcgttcatc
    10261 aggtcgcact caaccagtgc atcaaaaaat acggcgtaca gggacaatgc ggcttggaaa
    10321 cagtgtattg cacatcttct tcttattacg gcggaactgt gcgctctttg attcaaaatc
    10381 tcaaataaaa cggaaaatgc cgtctgaaag atgttcagac ggcatttcta tatcgacggt
    10441 caggattctt tcggatcggg cagcaggctg ttcaacataa tggcaagtac ggcgcacaag
    10501 cccacgccgg caaagctgaa gctgcccaat ttgagcgtca tgccgccgat gcccgtggtc
    10561 agtaccgagc tgacgatgac caggtttttc ggcagcatca aatcgacttt ggcatcaatc
    10621 agcgttttca cgcccaaaga agcaatcgtg ccgaacagca gcagcataat gccgcccatt
    10681 actggcatcg gaatggaagc caaaaacgca ttgaatttgc cgaaaaacgc catgcagacg
    10741 gcaaaaaccg ccgcccaagt catgatgacg gggttgctgt ttttggtaat catcaccgca
    10801 cccgttactt cgccgtaggt cgtaaccggc gggccgccga tcagacccgc aacgcatacg
    10861 cccaaaccgt cgcctgcaag ggttttgtcc aagcccgggt ctttcgtata gtctttcccc
    10921 gtcacattgc cgattgccat gatgccgccg atgtgttcga tggcgggggc gacggcaacg
    10981 ggcagcataa acagtgcagc ctgccagttg atctgaggcg tttcaaaatg gggaacggcg
    11041 aaccagggcg cgtgtgcaat gcttgccgtg tccaccagtc ccatcagcag tgccaaaaca
    11101 taacccgaag cgacaccgat caagatggga atcagcttca tcatcctgct gccgaaaacc
    11161 gatacgatgg cggtaacggc aaaggtaaag ccggaaagaa tcagcgaatc ggtatagtcg
    11221 atgacctgtt tgccgtccgc ctgacccatt gccatgctgc ttgccgccac ggcaacagac
    11281 agaccgatga ccatgatgac ggggccgatg acgaccggag gcagcagttt gtgtaccgct
    11341 gccagtccgc gccaacggat cagcgcggca aacacaaaat acataaagcc ggcggcaaac
    11401 agtccgaaca tggtggaagg cagcccccat tcgccgacgg agtagataat cggtgcgata
    11461 aaggcaaacg aagaaccaag aaaaatcggc actttgcgtt tggttgtgat ttggaacagc
    11521 agcgttccca agcctgcgcc caaaagcgca agagccggat tcagaccggt cagcagggga
    11581 accagcacca ttgcgccgaa tgccacaaat aaaatctgcg cgccggaaac ggcaagtttc
    11641 agttggttca taccatcttc tttcaatcgg ttcaaacggc acggattata ctggtttgcc
    11701 gagggtctgc gtaaaaatac atacgtcggg caacagatat gccgtctgaa aggcaatgtc
    11761 tgtctgttgc aggatgaaag caggagaaat ggagtataat ccgacacttt ccatattcaa
    11821 tccgcaagcc gtccggcttc cttatttcag acggcatttt ccggtaatac ggcatgacag
    11881 aaaaatccca taaaaaaaca gcaaaaggca gggcgggaag cccgtcccca acgtccgcac
    11941 gcaacaagaa agccgacaac ggcgcacggg gcaataaagt ttccgagcgg ctcaaggcgg
    12001 tcaaagagtt gcagaaaacc gaaaccaaaa aggcgcgtcc cgaacatgtc gtcaacctta
    12061 tcggcgacgc actgtggctg atgggtttgg cggcaaccct gtatttggcg atttccctga
    12121 tcagtttcga tatgggcgat ccgtcttggt cgcacagttc gccggttgtg gaagatgtcg
    12181 ccaattgggg cggactgttc ggcgcgtatg ttgccgatgt cggctattat cttttcggct
    12241 ggtcgttctg gtggtggata gcggctgcct gcgtcgtgct gtataaaaat ttccgcctgc
    12301 acgcaaaaca gacggaaaac gaggcataca accacaaaat cgctgccgcc gcgctgtttg
    12361 tcctgacggt cttcagcccc gtcttggagt attttgtgct gggcggaaaa tatgccgact
    12421 ccctgcctgt cggagcaggc ggtatggtcg gcatacgcgt cggcgcagtg tttgcgtggc
    12481 tgctggggaa atcgggcagc ctgctgatta tcttggttgt tctgctgttg tcgttgtccc
    12541 tactggtgca gatttcatgg ctggaatttt tgaacggtgc aggcagggcg gttcaaaacc
    12601 gcctgagtgc cttatccggc aaggtcatgg ctttaggaaa acgccggccg aataccaaaa
    12661 cagacggtgt cgatacccaa aatacacggc gcatggtaaa agaagccaag aatattacgg
    12721 ccaaacccgt tgccttgccc gaaggcagca gcagcaaccg caaatccgtc gcggtttccg
    12781 tcgcgccgcc gcccaaaatt caggtttctc tgtttgaaga tgacgaacct cggcaggcgg
    12841 gcgaatacca caagcctaca ttgaacctat tgcggattcc cgacagcgag cccgtcagca
    12901 tcaatcccgc cgaattggag cgcactgccg aactaatcga atccaagctg gcagaattcg
    12961 gcatcggcgt acaagtcgta tccgccacat ccggccccgt catcacgcgc tacgaaatcg
    13021 aacccgcgca aggtgtcaaa ggcagccaaa ttgttgcctt gtcgaaagat ttggcacgct
    13081 ctatgtcgct gcaatccgtg cgtatcgtcg aaaccatcgc cgggaaaaac acgatgggca
    13141 tcgagttgcc caacgacaaa cgccaagacg tgatgttgag cgaaatcttg tcctcgcccg
    13201 tgtttgccga agccaaatcc aagctgaccg tcgcgctggg caaagacatt gccggtaccc
    13261 ccgttgtcgg cgacttggcg aaaatgccgc accttttggt cgccggtatg actggttcgg
    13321 gcaagtccgt cggcgtgaac ggcatgatta tgtctatgct tttcaaagct acgcccgacg
    13381 aagtccgctt cattatgata gacccgaaaa tgctcgagtt gagcatttac gacggcattc
    13441 cgcacctgct ctgtcccgtc gtgaccgata tgcgcgaagc agggcaggcg ttgaactggt
    13501 gcgtcgccga aatggaaaaa cgctaccgcc tgctttccca cgccggtgta cgcaatctgg
    13561 aaggcttcaa ccaaaaagtc gaagccgcaa aagcggcagg caagcctttg cttaatccgt
    13621 tcagcctgaa ccccgacgag cccgagccgc tggaaaaact gccgttgatt gtggtcgtta
    13681 tcgacgaact tgccgacctg atgatgacgg aacgcaaagc cgtcgagcag caaatcgccc
    13741 gtctcgccca aaaagcgcgc gccgccggta tccatatgat tgtcgccacc caacgtccca
    13801 gtgtcgatgt cgttaccggc ctgattaaag ccaacattcc gacgcgtatg gcgtttaccg
    13861 tgcaaagcaa aatcgacagc cgtaccatcc tcgaccaaat gggcgcggac gaactgctca
    13921 aatatggcga ttcgctgttc cttcagcccg gcagtgccga accgacccgc ctgcaaggcg
    13981 cgtttgtttc agacgacgaa gtacatcaag tcgtcaacta cgtcaaatcg caagccccag
    14041 ccgactatat tgaaggtctg ctcagcggcg aggccgcgct ggaaactgcc aatatcgtta
    14101 atccgaatgc agacagcgac gaattgttcg atcaggcagt cgcctatgtt ttggaaagca
    14161 aaaaaacctc catttcgtct ttgcagcggc agttgcgtat cggctataac cgcgcggcaa
    14221 acctgatgga ggcactggaa aatgcgggtg tcgtttctcc cgccgacctc aacggcagcc
    14281 gtaaaatttt ggcgcacaag gaccatttgt agcccgtatt gcaaaatgcc gtctgaacgg
    14341 cggaattcgc gtttcagacg gcatatttat gtttcaggcg aaacattgtg atatacttgc
    14401 cagctaaaat ttcccctttg cggcaatgcg gttcaaatat cgtaccgttg cgctgtttgc
    14461 ttccccatgt agggaagaaa gtttatcatt ttatcaacac aacaaattta agggcttatg
    14521 atgagcgtaa ctgttgaaac tttagaaaat ctggaacgca aagtagtgtt gtccctgcct
    14581 tggtccgaaa tcaacgcaga aaccgataaa aaactgaaac aaacccaacg ccgtgcaaaa
    14641 atcgacggtt tccgtccggg taaagtacct ttaaaaatga ttgcccaaat gtacggtgcg
    14701 agcgcgcaaa acgacgtgat caacgagctg gtgcaacgcc gcttccacga tgttgccgtt
    14761 gcccaagagt tgaaagtggc aggcttcccc cgttttgaag gcgttgaaga acaagacgat
    14821 aaagaatctt tcaaagttgc cgccattttt gaagtgttcc ccgaagtcgt tatcggcgat
    14881 ttgtctgcac aagaagttga aaaagtaacc gcttccgtcg gtgatgccga agtggaccaa
    14941 actgtagaaa tcctgcgtaa acaacgcacc cgcttcaacc atgtcgaacg cgaagcccga
    15001 aacggcgacc gcgtcatcat tgactttgaa ggcaaaatcg acggcgaacc ttttgccggc
    15061 ggcgcatcca aaaactacgc cttcgtattg ggcgcaagtc aaatgctgcc tgaatttgaa
    15121 gccggcgtag tcggcatgaa ggctggcgaa agtaaagacg ttaccgtcaa tttccctgaa
    15181 gactaccacg gtaaagacgt tgccggtaaa actgccgtgt tcaccattac gctgaacaac
    15241 gtttccgaag cgactctgcc tgaagtcgat gcagattttg caaaagcttt gggtattgcg
    15301 gatggcgacg ttgccaaaat gcgcgaagaa gtgcagaaaa acgtaagccg cgaagtggaa
    15361 cgccgcgtaa acgaacaaac caaagaatcc gtaatgaacg cgctgctcaa agccgtagag
    15421 ctgaaagcac ctgttgcttt ggtcaatgaa gaagccgcac gcttggcaaa cgaaatgaaa
    15481 caaaattttg ttaaccaagg tatggctgat gctgccaact tggatctgcc tttggatatg
    15541 ttcaaagaac aagccgaacg ccgcgtatct ttgggtctga ttttggccaa actggttgac
    15601 gaaaacaaac tggaaccgac tgaagagcaa atcaaagccg ttgttgccaa ctttgcagaa
    15661 agctacgaag atcctcaaga agtgattgac tggtactacg cagatccttc ccgcctgcaa
    15721 gccccgactt ctttggcggt agaaagcaac gtcgttgatt tcgttttggg taaagccaaa
    15781 gtaaacgaaa aagctttgtc ttttgacgaa gtgatgggcg cgcaagcctg attatcctga
    15841 aaatgccgtc ttagggcagt ttttgaaagc accgaagccg cttttaaagc ccgctttggt
    15901 gcttttacat catgaaagga gacgaaatgt cttttgataa ctatcttgtc cctaccgtta
    15961 tcgagcagag cggtcgcggt gagcgtgcat tcgatatcta ttctcggctt ttgaaagagc
    16021 gcatcgtatt cttggtcggc ccggtaaccg acgagtccgc caatctggtg gttgcccaac
    16081 tgttgttttt ggaaagtgag aatccggata aggatatttt cttctatatt aactcgccag
    16141 gcggttcggt aacggccggt atgtcgattt acgacaccat gaattttatc aagcccgatg
    16201 tatcgacttt gtgcttgggg caggcagcaa gtatgggcgc gttcttattg tcggcaggcg
    16261 aaaaaggcaa acgtttcgcc ctgcccaaca gccggattat gattcaccag cctttaatca
    16321 gcggcggctt gggcggtcag gcatccgata ttgaaattca cgcacgcgaa ctgttgaaaa
    16381 tcaaagaaaa actcaaccgc ctgatggcga aacattgcgg ccgcgatttg gcagatttgg
    16441 agcgcgacac cgaccgtgat aatttcatgt ccgccgaaga ggctaaagaa tatggtctga
    16501 ttgatcaaat cttggagaac cgtgcttctt tacagtttta ataagaaatc tgagagaaac
    16561 aaatgccgtc tgaaaacagt tcagacggca tttgtcttat agagactgta ttgggatgat
    16621 tttgttctgg ttgcgtttgg agccgttttc gccgtagttg gttttgacat actcttcaac
    16681 aatagccaaa aattccgttg caatgttgtc gcctttcagc gttactttgc gttcaccatc
    16741 tacataaaca ggcgcgacgg gtgtttcccc cgtaccgggc aggctgatgc cgatgtcggc
    16801 caatttgctt tctccgggcc cattgacaac gcagcccatt acggcaacgt tcagggattc
    16861 aaccccagga taaagggtac gccatataga cattttttgg cgcaggtaat tttgaacatc
    16921 ttgtgccagc tcttgaaata cggtactggt ggtacgcccg cagccggggc aggcggtaac
    16981 catcggcgta aacgaacgca atcccatagt ctgtaaaatc tcttgcccga cgacgacctc
    17041 ctgagtacgc gggctgccag gttccggagt cagtgaaatg cggatggtgt cgccgattcc
    17101 ttcttgaagc aagacggata atgccgccgt tgatgcgaca atgcctttgc tgcccatacc
    17161 ggcttcggtc aaacccaaat gcagcggata ggcgcaacgg ctgcccagtt cgcggtaaac
    17221 ctgaatcaaa tcctgaaccg cgctgacttt gcacgacagg atgattttgt cttcgggcag
    17281 tcccaataga acggcttttt cggcagattc caaagcggag acaatcagtg cttccttcgt
    17341 cacttcttcg ggcggtttcg gcgcggaaga agcgaggttg gcatccatca tacgtttggc
    17401 gaggctttga tccaaagaac cccagtttac gccgatgcgg acggctttat cgttttcagc
    17461 agcagtccga atcataaagg caaatttttc atcgcctttt acgcctttgc cgacattgcc
    17521 gggattgatg cggtatttgg acaatgcttt gccgcattct ggaaattccg ccaacaggcg
    17581 ttcgccgttg aagtggaaat cgccgataag cggtgtggca tagcccatat cgtccaagcg
    17641 gcggcggatt tcggcaactt tggacgcggc ttcggggctg ttgacggtaa tacgcaccat
    17701 ttcggatccg gcatcgctca attccttaat ctgcaatgcg gtggcttttg catcggcagt
    17761 gtcggtgttg gtcatagatt ggataacgac gggtgcttct gaaccgacgg taatatgatc
    17821 gatgcggact tgatgcgtct tgcggcgttg gagtgtgttc atatggtttg aatctgttta
    17881 ttgaccggtg aggacggttt gcaattcttc cgagtagggg aaattcgcct gcaattgtgc
    17941 ttcatattcg tatgccgcct gtgcgttgcc gagggctttg gcaattttcc agcctagcag
    18001 caaatcatcg gcctgaagga cttctaccct gctttggtat tttttaaagt agtaatcggc
    18061 atcgcccaac tgcccggcca gcattttggt gcgcgccagt tctttaaatg cgggtgggaa
    18121 ctgcggctgg gcggcgaggg aacgtttcaa ataggcttcc gccaatccga attgcccctg
    18181 ttttgcgctg catatgcctt tattcaggtt ggcaatataa ggggtcgggt aggtggggtc
    18241 ggccagagct ttgtcgaaat atgccataga ttcggcaggg cggttgagcc tgccgcatag
    18301 aaaccaaccg tagttgttgt tgatttcggc actgtcgggt ttgatggaga gggcttgccg
    18361 gaaactttcc tgcgccttgt cattaacttt caggtattga tagatttcgg cacggaccag
    18421 ccaggcaagc tcgtttttag ggtctgattt caaggcatct tcaatacttg ccgtcgcctg
    18481 acggtagtcc tgaccgcgca tatattccat tgccaactgg gttttgatat tggaaacctg
    18541 attggctttt tctgcccgcg aggggcggta ggaagtgctg cacgcgccca aggcaaggac
    18601 aagtaataaa gagattcgtt tggatggctt aaaaggcata attacccctg ttgtccgatt
    18661 aaaatctgct gccatttttg ttggcggcgc gttttatcct gaacctgacc tgccaactgt
    18721 ccgcaggcgg catcgatgtc gtcgccgcgc gtttttcgta cggtaacgac aaatcctgcc
    18781 tgctgcaaaa tatcgcggaa cacacggatg ttctcattgc tggagcgttc gtatccggag
    18841 tttgggaagg gattgaacgg aatcagattg aacttgcagg gaacatcttt gactagttcg
    18901 atcagttcgc gcgcatgttg cgccctatcg tttattccgt ccaacatgac gtattcgaaa
    18961 gtgatgaaat ccctgggtgc tttgaccaga tagcgttggc atgcggccat caattctttc
    19021 aagggatatt ttttgttcaa cggtacgatt tggttgcgga cttcgtcatt ggaagcgtgg
    19081 agggaaaccg ccaaagccac cggcatgaca tcgcgcaacc tgtccatttg gggaaccata
    19141 cccgaagtgg aaacggttac acggcggcgg ctcaaaccgt agccgtggtc gtccagcatg
    19201 atgcttaagg cggtaacgac attgtcgaag ttcgccatcg gctcgcccat acccatcatc
    19261 actacattgg aaattacgcg ttcgtttttc ggtgtaacac ccatcgcttt gtttgcccac
    19321 cacaactgtc cgatgatttc ggcagcagtc aggttgcggt tgaagccctg ccggccggtc
    19381 gagcaaaatg tacattccaa agcgcagccg acttgcgagg aaatgcagag cgtgccgcga
    19441 tccgattcgg ggatgaagac ggtttccacc ccgttccccg tcccgacatc tagtagccat
    19501 tttcgagtgc cgtctgaaga ttcttgagac atcatcagct tgggaatttc gatgcttgcc
    19561 tgttcattta gtttatggcg caacgatttt gccaaatcgg tcatttcgtt aaaattttgc
    19621 gcgccggatt ggtgcatcca acgcataacc tgtttggcac ggaagggttt ttcgcccgta
    19681 tcggcaaaat gtcgggtcag cccttgaagg tcgtagttga gcagattggt tttcatgtgt
    19741 ttgtgttttt cttaaatcaa ggcttcaaat aaaaaattgc agggcaggca agtttgattt
    19801 gaagcctttt gcaacagagt tttcagacgg catggatata tttatgccgt ctgaagagga
    19861 gttgggcggt gtattgtatc aacgggggca gatttcggtt tggctgaaaa agtaggcaat
    19921 ttccaaagcg gcattttcca cgctgtcgga accgtgtacg gcattaatgc tgaccgaagt
    19981 ggcaaagtcc gcgcgtatcg tgccttcggc ggcttcggaa ggattagttg cacccatcag
    20041 ttcgcggttt ttcaggacgg cgttttcacc ctctaatacc tgaatcataa ccggaccgcc
    20101 ggtcataaat tcaaccaatc cggcgtagaa ggggcggtct ttatgaaccg cataaaattc
    20161 ttgcgcctct ttgagagtaa gctgcttcat tttggcggca acgattttca gaccgttctc
    20221 ctcaaagcgg ctgtatattt tgccgataac atttttgccg acggcatcgg gtttgatgat
    20281 ggagatggta cgttcaatcg ccatgctata tccttatttt tacttaagaa gaatcaaatc
    20341 ggtattctat caaaaaaata acttatccgc ctgaagcctt gggagatagg gtcaggtgcg
    20401 gctgttccaa ataaacttcg gactgcattt cgaccatccg gctggcggtg cgggtaaatt
    20461 cttcggcaaa atcgccttcc gtatagatat ggttgacatc taccgcgccg gtggcacaca
    20521 gtttgacccg gaaatcgtag agtacgtcaa tcagccaagt cagccgccgc gcctccgcct
    20581 tttcttgcgg tgagagttgt tccaaacctg aaataaaaac catttcataa tgttcggcca
    20641 aatacagata gtcggactgt gagcgggggc cgaagcacag tgcgcggaaa tcaaaccata
    20701 tggcacggcc ggactcggct ttgtggggaa tctcccgacc gtggatggtg ctgatgccgg
    20761 ggttcaaatc ggtaatgcct gtcatttctt tgaacagttt tgccagtttt gcctcatttt
    20821 cttcattggc aggcgtaaag aaaatctcgg cggggcggag ggtacgcagt cggtagtctt
    20881 caccgccgtc aacgtttaag acggtcaggc tggactcgat gagcgcgatt gtgggaagaa
    20941 aactgctccg gttttgacct tgcgggtaga gttcggaagg cgcgtagttt gaagtcgcca
    21001 ccaaaacaac gccctcgtta agcaggtttt ccagcagacg gcctaaaatc attgcatccg
    21061 caatatcgct gacatgaaat tcgtcaaaac acaatacgcg ggtttctttg gcaatctcgg
    21121 cggcaacgga tttcaacggg ttgctttcgc ttttcagggt tttcagcctc tggtggattt
    21181 ctgccataaa ggcatgaaag tggacgcggc gtttgcggcg gtaagggagg cagccgaaaa
    21241 aagcgtccat cagaaagctt ttgccgcgtc cgaccccgcc atagaaataa agccctttgg
    21301 ggacttgcgg ggaacgcaaa ctcctgccta aaaaacggtt tcttttgcgt ttgaacatca
    21361 tcaattcggt ccaaagccga tcgaggtgtt cgatggcggc tgcctgcgcg tcgtcgcgga
    21421 tgaagttggg cagttgtgag gcagcctgat accaggtcag cgggctgtgg ttttcaaacg
    21481 gcggggcttt aaaaagttgg tctctattac tcatatttaa ccttgttata cttcttgcgg
    21541 cggagaaaga aaatgcttac cgtcggtatt ataaatgatt tccttcaaac ttggcatttt
    21601 ccgtggaaaa gaataatgac gcgcatcagc aaaggcggcg ttccaatatg atgttgatat
    21661 cgccaattta ttgaaagaaa tgccttttgt ttcaggctgc gttttaatat tgaaggagga
    21721 caaatgaaac agatggtgaa tcgcgaagct gttacgccac cgtcagcttg gagggggaga
    21781 aaacacaaca ggctgcttat gtaaattcga caatgaataa tacgaagccc gaaagtgaag
    21841 taggaaatac gggtgatttt aatgtttgac caatcagggt ttaagcttaa aaatcatact
    21901 aatcaaatca agggcagggt ttggtaatcc gtagggtcgg ctccttccgg ttcagacggc
    21961 atacgtttat gttggtattt tgccggcact tcttaccgcc ctcgtgccgg ctatcccaaa
    22021 actgctttga acactgaacg ctcttcggtt tacttcaaaa acgcggcgtg ataagcaata
    22081 tgctcgccaa taaaactggc gatgaagtaa tagctgtgat cgtagccttt atggaaacgc
    22141 acatcgaccg gctggtttgc cgcacggcag gtttcgataa aatcttcggt acgcaattgt
    22201 gtcggcaaaa actcatcttc caagccttga tcgatgcgca taccttgcac tttatagcct
    22261 tgttgaatga gtgagttcgc atcatattgc tgccattttt cgcgatcttt tcctaaataa
    22321 gcagtaaagg ctttttctcc ccacggtacg aggcttggcg ataaaatagg cgaaaaggca
    22381 gaaacacttt gataatgttc ccgattccgt agtgccaata ccaatgcgcc gtgtccgccc
    22441 atcgaatgcc ccataatgga acgtttgccg ttggtaggaa agtgtttctc aatcagacgg
    22501 ggcagctcgt tcaaaatgta atcatacatt tgataattcg ccgcccaagg ctgttcggtc
    22561 gcattcaaat aaaagcctgc accctgtcct aaatcgtaag catcatcgtt cggcacttgc
    22621 tctccgcgag ggctggtatc ggaggccacc acaattactt gatgttctgc cgcataacgc
    22681 tgaaagcctg atttggtaat gaaattttgt tccgtacacg tcaagccgga aagccaataa
    22741 atcacaccaa gcggtcgatt ttctggatta tttggcaaat agacggcaaa tttcatttcg
    22801 cattgcagcg tttgggcatg atgcgcccaa acttgttgcg aaccgccaaa aatttgatgt
    22861 tgttcaatca gtttcatcgc ataccttagt agtgaataac ggcgcggatc gatttacctt
    22921 cgtgcattaa ctcaaaggct ttattgattt gatcgagtgt cattgtgtgg gttacaaacg
    22981 gttctaactc aatgtcgcct ttcattgaat cttccaccat tttcggaagc tcggagcggc
    23041 ctttcacacc gccaaatgct gaacctttcc aaacacgacc tgttaccaac tggaacggac
    23101 gcgttgaaat ttcttgtcct gcacctgcta cgccgatgat aatggattga ccccaaccac
    23161 gatgtgcact ttctaatgct tgacgcatta cgtttacatt gccgatacat tcaaaggtat
    23221 ggtcaatgcc ccatttgttg atgtctaaca acacatcttt aattggttta tcgtaatcgt
    23281 tcgggttcaa acaatccgtt gcaccaaact gttttgccaa ctcaaatttt gcgggattgg
    23341 tatcaatggc gatgatgcgg cctgctttgg cttggcgcgc accttgcacc acagccaaac
    23401 caatcgctcc caagccaaac acggcaacag agtcgccttc ttgcactttt gccgtattat
    23461 gcaccgcgcc aatacctgtg gtaacgccgc agccgagcaa acatacttgt tcatggttgg
    23521 cttcagggtt gattttcgcc agtgaaactt cggcaacaac ggagtattca ctgaaagtcg
    23581 aacagcccat atagtgataa atcggctgac cttgataaga aaaacgcgtc gtgccgtccg
    23641 gcattaagcc tttaccttgt gtatcacgca ctgagacgca caagttggtt ttacctgaac
    23701 aacaaaactc acattcgcca cattcggcgg tgtaaagcgg aatcacgtga tcacccggtt
    23761 ttacgcttga cacaccttcg cccacagcaa cgaccacacc cgcaccttcg tgtccaagca
    23821 ccacagggaa tacgccttca ggatcgcttc ctgataacgt aaacgcatca gtatggcaca
    23881 cgccagtgtg ggtattgcgg attaacacct cgcctttacg cggcatttct acgtcgattt
    23941 ccacaatttg taagggttgg ttaggggcga atgccaccgc cgcacgagat ttgatggttg
    24001 aatcggtttg tttcatttcc attgtctttc ctcttaattc attgacaaag ttgccttacc
    24061 tgagcaacat agcaacagtt tacactttag agttaactct aaagcaagtg ttttttactg
    24121 tttttgttta gaaggaggga aaatgactta taccactgcc aaagccgccg aaaaaatagg
    24181 catctccgcc cacaccctac gtttttacga caaagaaggt ttgttgccta atatcggacg
    24241 tgatgaatac ggtaaccgct gttttaccga taacgatttg caatggttgg gcttattgca
    24301 atgcttgaaa aatacgggga tgagcttaaa agacatcaaa cgctttgcgg aatgtaccgt
    24361 cattggcgac gataccattg aagaacgcct ttccttgttt gaaaatcaaa tagaaaatgt
    24421 gaagtgtcaa attgccgaat taaaacgcta tttagatttg cttgaataca aattggcgtt
    24481 ttaccaaaaa gcgaaagcat taggttcggt aaaagccgta aatttgccgc aaattcctga
    24541 aacggcttag ttttagtgca ataagcagga tttttgtcct gttcgtaata gaaatcaaaa
    24601 atttgttgtt taaaaagcgg attgtaagca atgatatagt ggattaacaa aaatcaggac
    24661 aaggcgacga agccgcagac agtgcaaata gtacggaacc gattcacttg gtgcttcagc
    24721 accttagaga attgttctct ttgagctaag gcgaggcaac gccgtactgg tttttgttaa
    24781 tccactataa aaaaatccac gctctaatcc gttgcttgct tggctcaagg tattgaagca
    24841 gcggatgaag ttaaaccaca gaaaaaacgc cgcgtaacag cggcgttttg atgctaaggt
    24901 ttaattggca ttttctttta aagccaagtc gacccgctcg cgcaattctt taccgggctt
    24961 gaagtggggt acatgttttt caggtacttc cacacgctcg ccggttttgg gattgcgacc
    25021 gatgcgggca ggacgatggt tcaaatcgaa gctgccgaaa ccgcggattt cgatgcgttg
    25081 acctcgggca agcgatctag tcatggtgtc aaccaagact tttacgctgt actctacgtc
    25141 ttttgccaga agatgcgtgc cgtttttggc ggcaaacact tctgccaaac gaaccattaa
    25201 ctcagacttt gtcatgtctg caaccttatt cttgttcgcc ggagagtttg gctttcagca
    25261 ggtcgcccaa gctggtggtg ccggcattcg cattggcggc ggcattgacg gagttcagtg
    25321 cttcgcggct ttctttggca tctttggctt taacggaaag tttgatgctg cggtttttgc
    25381 ggtcaacggt aacgatgacg gcttcaactt cgtcgccttc tttcagtttg gtggtcaaat
    25441 cttcaacgcg gtcggctgca aattcggaag caggcaggta gccttctact tcgtcagaca
    25501 gggcgataac agcacctttg gcgtcaacag atttcacgga acctttaacc aaagaacctt
    25561 tgtcgttcac gctgatgaag ttgccgaacg gatcgccttc cagttgtttg atacccaagg
    25621 agatgcgttc tttttccacg tcgattgcca atacgacggc ttcgacttct tcgccttttt
    25681 tgtatttgcg tacggcttct tcgccggatt cggtccagga caggtcggac aggtgaacca
    25741 gaccgtcgat gccgccgggc aggccgacga atacgccgaa atcggtaatg gatttaaccg
    25801 caccggagat tttgtcgcct ttgttgtggt tggcggcaaa ttcttcccaa ggattggctt
    25861 ggcattgttt catacccaaa gagatacggc ggcggccttc gtcgatttcc aaaatcatga
    25921 cttcgacttc gtcgcccagt tgtacgactt tgctcgggtg tacgtttttg ttggtccagt
    25981 ccatttcgga gacgtgtacc aaaccttcga tgccttgttc gatttcgacg aatgcgccgt
    26041 agtcggtcag gttggatact ttgccgaaca ggcgggtgcc ttgcggataa cggcgggtca
    26101 gaccgctcca aggatcttcg cccagttgtt tcatacccaa ggaaacgcgt tgtttttctt
    26161 ggtcgaattt caatactttg gcttcaactt cctgaccgac ttccaagact tcgctcgggt
    26221 gtttcacacg gcgccatgcc aagtcggtga tgtgcagcag accatcgatg ccgcccaagt
    26281 caacgaatgc accgtaatcg gtaatgtttt taacgatgcc tttgatgacg gagccttctt
    26341 gcaggttttc cagcagggct ttgcgttctt cacccaaggt agcttccagg acggcgcggc
    26401 gggacacaac aacgttgttg cgttttttgt ccagtttaat cactttgaat tcgatctctt
    26461 tgccttcgaa gtgagaagtg tcttttacag gacgtacgtc gaccaaagaa cccggcagga
    26521 atgcgcggat gctgctaatc ataacggtca ggccgccttt gactttgccg ttgatgatgc
    26581 cggacaggat gttgccgttt tccatggctt cttccagggc aatccaatcg gcagcgcgtt
    26641 tggctttttc gcgggacagt ttggtttcgc cgaagccgtt ttcgacggat tcgatggtaa
    26701 cggtaacgaa gtcgccgact ttaacttcaa tttcgccttg agcgtttttg aattcagcta
    26761 catcaatcag ggattctgat ttcagacctg cgtttacggt aacgaagttt tggtcgattg
    26821 ccactacttc agcggtaatc acctcacccg ggttcatttc ttgcagggta aagctttctt
    26881 ccaacagctg agcaaaattt tccatagaca tatataactc ttttcggtac accgccaagg
    26941 ggtgcggggt gggttggtgg atgtctgccg tccttggcag ggcagacggg taaataaggt
    27001 ttcagacggc atatggggaa attcatgctg tctgaaacgc aatgcgggcg attatacctg
    27061 aaaatttaaa ctttacgata ccaatcaagc acttttttta cagtttcttc tatagtcagg
    27121 cggctcgtat ccaaaagcag ggcatcgggt tgttgtttca ggggggcaac cttgcggttt
    27181 cggtctgcct cgtctctggc ttcgatgtcg gacaggatgc gctcgaatgc caaaccttcg
    27241 caggggatgc cgatttgttt ggcgcggcgt tcggcacgga ttttggactc tgccgtcagg
    27301 aagattttaa gttcggcttg ggggaagacg accgatccgg tgtcccgtcc gtcggcgacc
    27361 agtccttttt cggtcagaaa atcgcgttgg cgttgcagca gggcggcgcg gactttaggc
    27421 aactgtgcga ctgcggatgc gcccatgccg atggcttctg tccggatgcc gtctgaaacg
    27481 tcttcgccgt cgagcaggat gcggctgcct gaaaatacgg cgggcagttt ttttgccagt
    27541 ttggaaacgt tttcttcatc gtgccattcc acgccttgtt tttgtgcata tagggcggtc
    27601 aggcggtaga gtgcgccggt atcgagataa tcgtatccca atgcggcggc aacgcgggcg
    27661 gcaaccgtgc ctttgcccga tgcgcccggg ccgtcgatgg cgatgacttt ttgtctgttc
    27721 ataaggggga ttcctgatgg tttggggtat gggttttgcc gtctgaagga tgtgtttccc
    27781 gttggggcgg attctacctg ttttaaagga cgattgtcta agcagacgga acgccgcctg
    27841 cccgaaaaca tccgacagaa gccggcaagc cggttcggat tccggcgttg cgggtattgt
    27901 ggcgggcatc aaggcaatgc cgtctgaaag aggtatggcc ttcagacggc atcgattgct
    27961 gcagattaga acaggttgct gacaaaaatc agcaaaatca ccaacggcac aagatatttc
    28021 acataagcaa accaaatatt gaccgtcgta tggttgcctt tgtaaagcaa ttcttccttc
    28081 gcttcgtcct tcatcacaaa accgacaaac agcgcgaaac cgagcgcggt cagcataaac
    28141 aagatgttgc cgctgatgta gtcgaaggca tcgaaaatat ttttgccgaa cacggaacgt
    28201 ctttccacga accatagctc agaatggacg ggatgttgcc gaaaatgaag atggcggcta
    28261 atacaatcgt aatcgcggcg gtacggcgga ttttggtttt ttcctgaatg gtcgtaatca
    28321 acacttcata aatggtcagc gaagtcgtca atgcggcaat caggagcagc gagaagaaaa
    28381 tcacggcgaa cacagatctc gcccacatat gtgagaacac aatcggcaag ctttggaaca
    28441 ccaaagtcgg gccggaatcg ggggcaacgc cgaagctgaa gagcgacggg aaaatcataa
    28501 agcccgcaag tatggcgatg atggtattgg taattgccgt gataactgcc gtctgaacca
    28561 gattttcgtt tttatccaaa tagctggaca aggtaatcat cacgccgaaa cccaggctca
    28621 gggcaaaaaa tacctgcccc aaaacgaaga cgaacagttc ggcggtaatc ttgctgaaat
    28681 caggtttcag atagaaagca accccttcca ttgcgcccgg aagggtaacg ttgcggacga
    28741 ccatcgcgat taggaacaaa aacagcagcg gcatcagcta ttttgccgct ttttcaatgc
    28801 cgccgataac gcctttggcc aaaatccatt ggttcacggc gacaaaaagc agcgtataaa
    28861 acgcaatttc ccaagggctg tttcaatgtg ttcggcaaag aagccttttg taaccacacc
    28921 gtcgacgggg ctggaaatat tcaaatttcc tccaataata ttaacgatat agctgattac
    28981 ccagccgccg agtaccatgt aataagccat gatgccgaac gcgccgagca gcccatccag
    29041 ccgaccagtt tccaaatttt ggcaatgggt ttgccgttca tcgggccgcc gaacgcatcc
    29101 agcgcgttca cgcctttgcg ccgtccgatg acattttcca ccaaaatcat cgggatgccg
    29161 ataaccagca tcgcgataca gaataaaaac acatacgcgc caccaccgtt ttcaccgacc
    29221 aaatacggga aacgccacgt cgcgccgaaa ccgacagtcg cgccggcaat ggtcaggata
    29281 taggttaatc ggctggatca ggtttgacga ttggtatttg aaggggaaga catattgaaa
    29341 ccgtgtccga ttgagataaa gcggaaattc tacacgcgtt ttttaacagg aactgactga
    29401 ttgtttttca actgtctagt gattttccat gtaaaaggca tatatttcag tgagtatttc
    29461 tgatgagtat aacccgatga ggaagaatcg gagtttataa atagattaat ttgttattct
    29521 tctacatcgg tgtatggaaa tgaaattttg ttaattatat taatgatagc atttaaataa
    29581 tgatgaaaga gagggaattt gaaatatagt ggattaacaa aaatcaggac aaggcgacga
    29641 agccgcagac agtacaaata gtacggcaag gcgaggcaac gctgtactgg tttaaattta
    29701 atccactata aaaaaaccat ctatacaagg ggagaagtat agatggcaaa acacattacg
    29761 gtgaaaacgt cttactcata agcctgcttg aacaggcgtt actcagacaa atggattata
    29821 tcgtaaattg attttttgcg ttaaattggg ttaaaccata tatttaatga gtatgtggat
    29881 gaatataaat ggaattcttg gatgttattt cagaattttg gacattcgat aaatttccat
    29941 tccccgggtt tgaggttttc tgtttccaga tgtgcgaatc gccggcgatg aaggtgttgc
    30001 acgcggttgc cggcggcggc gatcatgcgt ttgacttggt ggtattttcc ttcggtaatg
    30061 gtcagcagca gggtggtcgg gtttttcaaa acggcatcgg cggcacaaac ggtttcgttt
    30121 tcgtcgtgga gcagcacgcc gtttttcaag gtttcgcaga gcgtttctcc tgtggggtgt
    30181 ttgagcgtta cttcgtatag cttgggaatt tttctgctcg gcgaagtcag gttgtggttc
    30241 agtttgccgt cgttggtaat cagcaatacg ccggtcgtat ccgcatccag cctgccgacc
    30301 gcctgcatat cgatgttccg catattgtcg gggaacaggc tgaatacgct gcggtagtgc
    30361 ttgggtttgt gcgaagtttc gtaatcttca ggcttgttga gcatgatgta gaaatagggt
    30421 tcgggaacga cggttactgc ttccccgtca atatccaacg tttcgacgga tgaggaatcg
    30481 atgtctgcat cggtgtcgtc catgcaggtt ccgttgatga aaacataacc gccggcaatc
    30541 agccattggc actgcttgcg gcttcctatg ccttgatatt gcaggtattt gataagtttc
    30601 atgatggtat gggaaatgtg ggatgaaaaa gacaggactg agaaagtcct gtcatgtgcc
    30661 gtatccgaat cagcttccaa tcagtttgac cctctgtccc ggattgatgg tgttcaggtt
    30721 gggattgagc cggcggatgt cgtcgatatg gatattgaag cgcgcggcga tacttttgaa
    30781 ggtgtcgcct ttgcgcgcgg tgtaggatac tttttcaata cgggtttggg caggggctgc
    30841 ctgcgccagg cggagtacct gtcctttttg gatggtgttg cctttgatgt tgttggcgac
    30901 aatcaggtcg gctacactga cgttgtagcg tttggcaatg ttgaacaggg tgtcgccttc
    30961 tacaacgcgg tggatgctgg catggagcgg ggagatttcg gcttcgccgt cggcagcttt
    31021 ggctacgcgc gtttccaaac gtacccggcg tttgccgtcg gcggcaacgg tatgttgcgg
    31081 agatgaggaa atgttgtgtt cggcaactgt gtcaggcgtg ccgatgacgg cggagatggt
    31141 ttcttcagcc tgtcggcgca ggttgtttcg ggcaacaagc tgcatgagtt cgtctgccgc
    31201 ctgcatgtcg ttttggggga cgatctgcgc gagtgttgcg gtttgggttt cagacggcat
    31261 ggcggtctgt ttttcggttt gaggttgcgc tgcggctgtc gcaagggcag gttcggcaat
    31321 acggacaagg ggatcgggtt ccgtacggac ggttttctgc ggcaaaggtg cgacggtaat
    31381 gtccgctgtc tgtgcggcgg cgggtcggat tcgggcaatg ccgacgttca ccgtgcctgc
    31441 cggcatattg gaacggtagg tgtcgggtgt attgtcgatg tcgatggaaa cgacggattc
    31501 cgatgccgtc tgaagggttt tgccgttctt ggcgacaagg atgctgcgtc ctgcgttgac
    31561 aaggttgccg ttcaggttgt tgaggcgttt gatgtcggca atgctcatgc cggttgccgt
    31621 cgagatgtcg gacaggctgg ttttggcggc aggtgtatag acttcccatg aaaacaggct
    31681 gtcgggtgcg gcgttgaggt agttgctttg gaaggtttgt acggacgcga caggaagcag
    31741 cagtttgcgt ttgcttttgg ggataaacgc ggggacgttg aatgcggggt ttagggcgag
    31801 cagctcgctt tgcgtgatgc cggcaagccg ggcgatggct tcgttgtcga gcggacgatc
    31861 cggttcgact gcctgaaaat agggtttgtt gtctatgtcg ctgatattca tgccgaaaga
    31921 ttggggagtg gcaatgatgt tgcgcacggc gagcagcttg gggacatagt tgcgcgtttc
    31981 gttgggcata cgcaggtttt cgtaggtcgg ttcgagccct tgggcgcggg cgcggttgat
    32041 ggcgcgtccg acgttgcctt caccccagtt gtaggcggca aaggcaagcg gccagtcgcc
    32101 gaacagtcca tagaggtatt gcagatagtt gagtgcggca tcggtggcgg cgtaaacgtc
    32161 gtgcctgccg tcgtaaaccg gtgttttttc caggccgtaa tgcctgccgg tagcgggcat
    32221 aaactgccat aatcctgatg cgccgacgtg tgatttggct ttggtgacga acgcgctttc
    32281 gatgaaggga agcagggcgg cttcggcggg catattgcgt tttttgactt cgttggcgat
    32341 atggtacata tagggtctac tccggttgat gaccctgttg aaatagctgt ggcttgcgat
    32401 gaatttgctt tcgtggcggc gtaccagttc gggattgact tcgcccatcc ggaagccttg
    32461 gcgcagctcg ctccacaggc tgccggattg gaaatattgt tttgtcgggg gcaggtcgag
    32521 tattgaagag tttaagcgca taatcgccaa accgatttgg tgtgatgagg tgttttgggc
    32581 gtataggaaa cccggacaaa cggacagacc tgatgcggtc agagcgatgg ttttgagttt
    32641 ggacatgatt tatattcggc ggaataaaca tattttatcg tccgatggta ctgtttgact
    32701 gaaaaagcgt caagttttac aggaacttta tgcggattcg gaagtgggcg gccgatgtgt
    32761 tttttgacag aaattctgtt tggaatagaa tatcggcagg caatctgttt gaaacggacg
    32821 caatatggat gcatggtttg aagatacggc gatggggcgg tatgttgcaa aattggaaca
    32881 ggatttcttc gggcggtatc tggattcata ccgtttttcg ggaatgtgtg cggttcaggt
    32941 gggcggcagg tggctgagcc tgtctgaaga tgttgtctgt gtgccgcgag atatgtcgat
    33001 gtcggcggag aatatggctt tggcggatgt ttctgcggat atgctgcttt ttccgcatac
    33061 gcttgaaggc ggtgttcctt cgcaaatcct gtcggaagcg caccggatat tgaaaccgtc
    33121 cggacgtttg atgctgacgg gtttcaatcc gtattcgctc tggggattca gttgttggtt
    33181 tgacggcgaa cgcctgccgg aaaaacggtt ttgtctgccg ctgcccgagt tgaaaagacg
    33241 gcttgcggat gtcggtttcg atattgaatt tgggaaattt atggtgtatc tgccgccggt
    33301 ttcgtcgctc gggcaaatac gcttttggcg gtttatggaa aaggcgggcg accgttggtg
    33361 gccgcagtgt gccgcagtgt acggtttggt tttggtcaaa agggcggcgg gcgtaacgcc
    33421 cctgcccgcg tgggacgggt atttgggcgg caaagccctt gcggcgggtg cggcaagggt
    33481 tgcggattag ggacagccgt ctttcagacg gcatctgtca gaagaaatgt cgggaggggt
    33541 tatgcctgaa ttgccggaag tggaaacgac gttgcgcggc atcgcgccgc atattgaagg
    33601 gaaaacggtg gaagccgtgg tattgcgcca attgaagctg cgctggcaga ttaatcccga
    33661 tttgggggag attttgtccg gccggcaggt gttgtcctgc ggcaggaggg cgaaatacct
    33721 gcttatccgc tttcaaacgg gcgtgctgct gattcacttg gggatgtcgg gcagcttgcg
    33781 gatttttacg ccgtcggacg gacgtatcgg caggccggac agacacgatc acgtcgatat
    33841 tgtgttttca gacggcacgg tcatgcgtta ccgcgatccg agaaagttcg gcgcgatact
    33901 ttggtatgag ggaatcgaag aacatcatcc gctgttggaa aaactggggc cggagccttt
    33961 gtcggaggca ttttgcacgg attatctgta tgtgaggctg aaggcgcaga agcgcgcggt
    34021 caaacttgcc ctgatggaca atgcggtcgt ggtcggtgtg ggcaatattt atgccaatga
    34081 gagcctgttc agagcgggca tttcgcccca ccgtcctgcc aaccgtctga aaaagaaaga
    34141 gtgtgcgctt ttggttgaaa ccgtcaaagc ggtgttgcgg cgcgccattg aaacgggcgg
    34201 cagtaccttg agggatttta tggacagcga cggcaaaagc ggctacttcc aacaggaata
    34261 tacggtgtac gggcggcaca atcagccgtg cccccagtgc ggcggtttgg ttgtgaaaga
    34321 aactttgggg cagcgcggca cgttttactg cccgaattgt cagaaatagg gcggaaaacg
    34381 gtttcagacg gcattttatc ggtatgccgt ccgaacgttt caacaacaaa caccgattat
    34441 cgggaaagaa ttgttcatgt cttcaaataa agcttcattt tttacacgtc tgcgccgctt
    34501 gtgccgcttg acggtctggc tattcaaaac cggcaaaaac ctgcgcggta ttgacggcgg
    34561 ctgccccgag tcgcgcaatc gggcggtaat cgagttgggc aggggggctc tgacggcttt
    34621 ggatatcgga ttggaggtgg gcaggcccgc acccgaacat ccgaacggtg tcttggttgc
    34681 cgccaaccac gtgtcctggc tggatatttt cgcgatgagc gcggtttatc cgagcagctt
    34741 tatcgccaag caggaaatca aaagctggcc ggtattgggc aagatggggc agaacgcggg
    34801 aacggtgttc atcaaccgca attcgcggcg cgacatcgaa ccgattaacc gcgccgtctg
    34861 cgaaaccttg caacgcggtc aaaacgtcag ttttttcccc gaagcgcgga cttcctccgg
    34921 actggggctc ctgccgttca aagccgcgct gttccaatcc gccattgatg cgggggcaaa
    34981 ggttttggcg gtcgcgctgc gttattatga cgaaacggga aaaaggacgg ctcgtccctc
    35041 atatgccgat gtcggtttgc cgacctgcct gtggcgcatt gtgtctatga aaaaattaac
    35101 gataaaagtc gatttcgttt gcgtggcgga tgcggcggaa agcgaagacc gttatgcttt
    35161 aaaagataaa atcgaagaaa gcatccgtgc cgttgtcgcc gacgatgcgg atatcgctgt
    35221 ctgaaaccgg ttgccggaat gtggcagtat gattcgcttt tgcggatgta tagtggatta
    35281 acaaaaacca gtacagcgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct
    35341 gaagcaccaa gtgaatcggt tccgtactat ctgta
//
LOCUS       DAWFHY010000010        35230 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.010, whole genome shotgun sequence.
ACCESSION   DAWFHY010000010 DAWFHY010000000
VERSION     DAWFHY010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 35230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35230
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.010"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(120..677)
                     /locus_tag="WGQ68_000427"
     CDS             complement(120..677)
                     /locus_tag="WGQ68_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0714296.1"
                     /translation="MKPKFKTVLTALLLAVSLPSMAATHVLMETDMGNIRLVLDESKA
                     PKTVANFVRYARKGFYDNTIFHRVIGGFVIQGGGLTEDLAQKASDKAVANESGNGLKN
                     TVGTIAMARTTDPDSATSQFFINLADNDSLNYKNGQYGYTVFGRVESGMDTVSKIARV
                     KTATRGFYQNVPVQPVKIRRVVVGQ"
     gene            complement(742..1668)
                     /locus_tag="WGQ68_000428"
     CDS             complement(742..1668)
                     /locus_tag="WGQ68_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0714297.1"
                     /translation="MSEMKKTKVHLISGFLGTGKTTALKSLMAQKDPNEKWVIIVNEF
                     GEIGIDGAVLSDNGIPVAEIAGGCLCCTAGPQMGVTVQKMLRDAKPDRLMIEASGLAH
                     AASVIDELKAKPLDSLLEIGAVFTVVNPRQFINPDYAQQALYKDQIGICDVLVASKTD
                     LCTTEQLAEFHDKAAKLFPPKAKVVEVQNAQLDIRWLDIPVIEKSRYRLKALPDNTMG
                     FQSQGFTFPAGRDFDGEKLTNFFNDLPKMTEGLVRAKGVFQVLGTWVWLNWVDGQWGA
                     NQVSWRRDSRFELIAKSFDADLIEQKLKDALE"
     gene            complement(1656..2132)
                     /locus_tag="WGQ68_000429"
     CDS             complement(1656..2132)
                     /locus_tag="WGQ68_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEZ0714298.1"
                     /translation="MKTNFKQKIIEQARSEGLQVTALREQVLDIVLQQSGVIKAYNVL
                     SQMQQQSEGVLAPPTAYRALDFWAEQGVLHKVAAVNGYILCSHAQHECDDHCHDHEEA
                     EAHHSAFILVCTECGTADEQTLSHEWAALRAGVAESGFALKEEHVVLTGICKKCQK"
     gene            complement(2203..2658)
                     /locus_tag="WGQ68_000430"
     CDS             complement(2203..2658)
                     /locus_tag="WGQ68_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEZ0714299.1"
                     /translation="MKKPKILFVCLGNICRSPMAEYILRRRAAEAGIPLEADSAGTSG
                     WHDGEDMHRETAKILKKHGIDASGFTSRKIRQSDAAAFDCIIAMDGKNLSELERTFGR
                     RPEKIFKLTDLIPESGYDHVPDPWYTGDFEETFRLADAGCRALLEKISK"
     gene            complement(2783..3898)
                     /locus_tag="WGQ68_000431"
     CDS             complement(2783..3898)
                     /locus_tag="WGQ68_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEZ0714300.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQAIADVECLLCPVGDG
                     GEGTVDAIRHSLDLEEKCLQVTGPFGQKEVMRYFQKEQLALFEVADLVGLGKIPLEKR
                     NPLQIQTRGIGELIRHLISQEIKEIYIGVGGTASNDGGIGIAAGLGYQFYDEDGNTLP
                     TCGQALLNLASVSTENRYEIPEDVHIRILADVVSPLCGHQGATYTFGKQKGLDSTMFE
                     AVDQAIQDFYEKVSPATLKLKGAGAGGGIAGGLCAFAQASIVSGIDTCLDLIDFDKKV
                     SDVDLVIIGEGRLDRQSLAGKAPIGVAKRTPVGVPVVAICGSLVEDLPSLPFENIQAA
                     FSILEKSEPLEDSLKNASLYLEHTASNIGHLLNMPKI"
     gene            complement(4012..5109)
                     /locus_tag="WGQ68_000432"
     CDS             complement(4012..5109)
                     /locus_tag="WGQ68_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEZ0714301.1"
                     /translation="MNQTFTLPDTRPYPQNPIKNHLLLNAYQLAHNSSQASRKLSSGQ
                     LQTEIRGMLEQNHYINLSLALTMSPDAGTYAALLSSVNAVLDCEKEGEVQWFALPVVL
                     VSGCKKERAIEMKLPTEALFACLQNYPHLRALAQETQWLPYLVHSSDLSAVAPDEWWR
                     AKQNTEAAAQHLRRFAPRPLLLPEGQSVHVVYALGFGSGKVQTALGQNLLQAGLPLMQ
                     VWQENLASEGVTLFANPLSPDSPVRALSEGSHTRQRMAMDVFAANAIRAVRMQSPRVG
                     VVAAAKVGGQILFGFNATDGAFEVVPQVFSWQLSFTDNIAVVQQNFLDLMAECRVEHV
                     YLLHNPLGEQESIPSYAEALKREGHNPFFSA"
     gene            complement(5191..6774)
                     /locus_tag="WGQ68_000433"
     CDS             complement(5191..6774)
                     /locus_tag="WGQ68_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase regulatory domain-containing
                     protein"
                     /protein_id="HEZ0714302.1"
                     /translation="MPNKKKKAAFARAATENGVPDVSNIKNSYDDLMYESKAFSQTAI
                     NALEARARMMGLKPAAAAKAKVLELGCSMGGNIITQALYYPDAEFVGIDLSGRQVAQG
                     NAIIEKMGLKNVRLEEKDILTIDESFGKFDYIIVHGIWSWVPDAVKDKIFSICRNNLT
                     EHGIAYISYNVYPGWKRQEQLREIMYFAGRDVLEEPLEARTRKGLDALKALAEILEND
                     KGLDGGGKLPAIQKILNHNFYYIAHEYMEAFNDPIYVNGFIEWANRHRLAYIGDTDLH
                     ASFVSWMAEHTRERILALAGGDYIAKEFYSDILSDRQFRRSLLCREEVGDNIRRDESV
                     AVEVIESLNFRPARGETINFDENDTLLSGIRDVMKTGEAFKTEDVAENLARRFPGLEF
                     DRMKINSQLLLQTILGRFSVSSDNAGKPFFEDHKTYVPARFTDYAAAFVEHGAEAFVR
                     PANRYNESTPSFGYGHLYIMRQLSRPTSKQAVIDAVAENLNIVSTTPDGLTFHPPAEV
                     YVEEILADLADRHFLVSAD"
     gene            complement(6922..7134)
                     /locus_tag="WGQ68_000434"
     CDS             complement(6922..7134)
                     /locus_tag="WGQ68_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEZ0714303.1"
                     /translation="METLILDIGGMSCGGCVKSVTRILEGVKGVASVEVSLENKSATV
                     GYDPAQTDAGALIEAVEDGGYDAALK"
     gene            7480..7635
                     /locus_tag="WGQ68_000435"
                     /pseudo
     CDS             7480..7635
                     /locus_tag="WGQ68_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713084.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
     gene            7765..9201
                     /locus_tag="WGQ68_000436"
     CDS             7765..9201
                     /locus_tag="WGQ68_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HEZ0714304.1"
                     /translation="MPLLSVEFALFFLAFLPIYWGLAKYPSVQNLLLLAAGMGWLYHI
                     GPVFAAIVVLYSSCVYLLGELLRSDRENTRRFWLGCGIAVSLTVLGFFKYFDFFRPLI
                     AQYAGQGGAIDILMPLGLSYYTFQSLAYLVYCFRAPHAARFEWHELLLHLSFFPTVTS
                     GPIIRAAAFKSADGEQAGALAQIRTRQPRSPVRPALAVSLILLGIAKKWWLAGMLAEN
                     WVSPVFENPAQFDGWGVLGGVYGYTFQLFLDFSGYSDLVIGMAMLLGFRLPKNFSAPL
                     RALNIRAFWDKWHISLSTWIRDYIYIPLGGSKKGFLRTQLNLMAAMVLSGIWHGYGWN
                     FLIWGALHGTALVLLNTGDRYFGRNALCRLKYLAPLSWFVTFHFVCLSFVVFNTASPD
                     DAGAVFNALFANANGWNAPQQANMLLLASFASVMLLYPYLQRAFDGAVKGLEKIPMWL
                     WFIPVSAVLLLIIVLAPSGIPGFIYANF"
     gene            9212..10195
                     /locus_tag="WGQ68_000437"
     CDS             9212..10195
                     /locus_tag="WGQ68_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF459 domain-containing protein"
                     /protein_id="HEZ0714305.1"
                     /translation="MKNFLSLFTSILMSALIAVWFSQNPINAYWQQTYHRNSPLEPLA
                     AYGWWRSGAALQENAYTLSDGIKAFLSGETPPTAQDGGSADMPSEAATSEAVPQTGET
                     EWKQDTDTAAVRSGDKVFFVGDSLMQGVAPFVQKSLKQQYGIESVNLSKQSTGLSYPS
                     FFDWPKTIEETLQKHPEISVLAVFLGPNDPWDFPVGKRYLKFASDEWAQEYLKRVDRI
                     LEAAHTHRVQVVWLGIPYMKKAKLDGQMRYLDKLLSEHLEGKIILIPTAHTLSGGEDR
                     HTDSVNVNGKPVRYRSKDGIHFTAEGQKLLAAKIMEKIVFEPSTQPSSTQP"
     gene            10192..11382
                     /locus_tag="WGQ68_000438"
     CDS             10192..11382
                     /locus_tag="WGQ68_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEZ0714306.1"
                     /translation="MNPKHLIAFSALFAATQAEALPVASVSLDTVTVSPSAPYTDTNG
                     LLTDYGNAAASPWMKKLRSVAQGSGETFRILQIGDSHTAGDFFTDSLRKRLQKTWGDG
                     GIGWVYPANVKGQRMAAVRYNGNWQSLNSRSAAADFPLGGILAHTGSGGSMTLTASDG
                     KTGKQRISLFAKPLLAEQTLTVNGNTVSANGGGWQVLDTRAALPLTIQTEMPWDIGFI
                     NIENPAGGITVSAMGINGAQLTHWSKWRADRMNDLAQTGADLVILSYGTNEAFGDNID
                     IADTEQKWLDTVRQIRDSLPAAGILIIGAPESLKNTLGVCGTRPVRLTEVQQMQRRVA
                     RQGQTMFWSWQNAMGGICSMKNWLNQGWAAKDGVHFSAKGYRRAAEMLADSLEELVRA
                     AAKR"
     gene            11587..13140
                     /locus_tag="WGQ68_000439"
     CDS             11587..13140
                     /locus_tag="WGQ68_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid--CoA ligase"
                     /protein_id="HEZ0714307.1"
                     /translation="MNRTYANFYEMLTAACRKNGNGTAVFDGKEKTTYDALKQEVEAV
                     AAYLQNIGVKFGDTVALAVSNSTEFITAYFAVSAIGAVAVPMNTFLKNSEYAYILNDC
                     KARFMFASAGLSKELAGLKAQTPVEKIIWTGKSRQGGGAAEGDAFFEDVRRFPEKPDL
                     GHQPRINDLAHIIYTSGTTGHPKGALISYANLFANLNGIERIFKISKRDRFIVFLPMF
                     HSFTLTAMVLLPIYMACSIILVKSVFPFSNVLKQALLKRATVFLGVPAIYTAMSKAKI
                     PWYFRWFNRIRLFISGGAPLAEQTILDFKAKFPRAKLLEGYGLSEASPVVAVNTPERQ
                     KARSVGIPLPGLEVKAVDEELVEVPRGEVGELIVRGGSVMRGYLNMPAATDETIVNGW
                     LKTGDFVTIDEDGFIFIVDRKKDLIISKGQNVYPREIEEEIYKLDAVEAAAVIGVKDR
                     YADEEIVAFVQLKEGMDLGENEIRRHLRTVLANFKIPKQIHFKDGLPRNATGKVLKRV
                     LKEQFDGNK"
     gene            complement(13231..15258)
                     /locus_tag="WGQ68_000440"
     CDS             complement(13231..15258)
                     /locus_tag="WGQ68_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HEZ0714308.1"
                     /translation="MSLSEFIERRTSFNPMVILTTLFFVCVLVVLVLTVPDQAQMWLD
                     RAKEVIFTEFSWFYVLTFSIFLGFLLILSVSSLGNIRLGRDEDVPEFGFLSWLAMLFA
                     AGMGVGLMFFGVAEPLMHYFSDITAGTPEHRQQQALLHTVFHWGVHAWSVYGTIALAL
                     AYFGFRYKLPLALRSCFYPLLKEKISGRFGDAIDIMALLATFFGIITTLGFGASQLGA
                     GLQEMGWIAENSFSVQVLIIAAVMSLAVVSAISGVGKGVKVLSELNLSLAFLLLFFVL
                     AAGPTVYLLSAFGDNIGNYLGNLVRLSFKTYAYEREHKPWFESWTVLYWAWWCSWAPF
                     VGLFIARISKGRTIREFVFGVLLIPGLFGVLWFTVFGNTAIWLNDGVAGGVLEKMTSS
                     PETLLFKFFNYLPLPELTSIVSLLVISLFFVTSADSGIYVLNNITSRDKGLSAPRWQA
                     VMWGVLMSAVAVLLMRSGGLGNLQSMTLIVSLPFALLMLIMCFSLWKGLSADKKYFET
                     RVNPTSVFWTGGKWKERLVQIMSQTQEQDILKFLKHTASPAMHELQRELSEEYGLSVR
                     VDKMFHQDEPAIEFVIRKETMRDFMYGIKSVGQDVSDQLINDGKLPHIRHQTTYKPYA
                     YFFDGRVGYDVQYMNKDELIADILKNYERYLMLLDDVGQELMAHEQVELAE"
     gene            15547..17553
                     /locus_tag="WGQ68_000441"
     CDS             15547..17553
                     /locus_tag="WGQ68_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEZ0714309.1"
                     /translation="MADMKKITPQNLRPLLSESLGHTDFVNVLNALIKFLRRGGKKCA
                     GERFDLIIDTFKQDRELLSRFSRCFYIWLAQIHIYPALIKLGIFSRHSFAREMGIRIY
                     ERFSPSYKDFANLGEVFLYLFHSENDDKWLQTLNIRQWLVLYELIRSHAEPSKLQTAG
                     IRLADARLRAIEMLSVWTASEAIEPDLIRIAPRLLEADSSFVALQRETAKLVEHYRNG
                     TTPYDTAHLEVMFDQCFSQIDYLRRKGTGAGSGSSVKVAHLLERLRQTVDRLKLLTDI
                     QTGVGNSNRLTIALMNSLIYAAVEQYSTRHLRRSSIRMLARSITENKSHHGEHYITRN
                     RKEYFKMFYSAAGGGVIIALMALLKIRIGTLGLSPFLTSLSAGFNYGIGFMIIHILHC
                     TVATKQPAMTAASFAEQVDLNEGGKAVDNKLAKLLIDVCRSQSVAVFGNVSIAILLAC
                     AISFGYAHLHQQPILDAHTAAYQFKSIDIIAYPTLWYAAIAGLWLFCSGIIAGFFDNR
                     ADYLNLRQRLPFNPLLRKIMRPGPRRVLAAYIHKHYGSLAGNFIFGMLLGMTGYFGHL
                     LGLPLDIRHVAFSSANLGYAAVSGNVGLGTFVLGIFSVLAIGLVNLCVSFSLALFVAL
                     RSRGTKIGSIRNLIKSFWNQIKSNPCILFLPPAKEQGHPPSDKP"
     gene            complement(17781..18872)
                     /gene="mltB"
                     /locus_tag="WGQ68_000442"
     CDS             complement(17781..18872)
                     /gene="mltB"
                     /locus_tag="WGQ68_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HEZ0714310.1"
                     /translation="MKKRKILPLAICLAALSACTAMEARPPRVNEAQAPRADEMKKES
                     RPAFDAAAVPVSDSGFAANANVRRFVDDEVGKGDFSRAEWQDFFDKAAYKADIVKIMH
                     RPSTSRPWYVFRTGNSGKAKFRGARRFYAENRALIDDVAQKYGVPAELIVAVIGIETN
                     YGKNTGSFRVADALATLGFDYPRRAGFFQKELVELLKLAKEEGGDVFAFKGSYAGAMG
                     MPQFMPSSYRKWAVDYDGDGHRDIWGNIGDVAASVANYMKQHGWRTGGKILVSATLAP
                     GADVQAIIGEKTALTRTVADLKAYGIIPGEELADDEKAVLFKLETAPGVFEYYLGLNN
                     FYTVWQYNHSRMYVTAVRDIANSLGGPGL"
     gene            complement(19021..20448)
                     /locus_tag="WGQ68_000443"
     CDS             complement(19021..20448)
                     /locus_tag="WGQ68_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0714311.1"
                     /translation="MIHTEPSAQPSTMDTAAFLKHIESAFRRIFSDGIDLMRYLPEDK
                     WLALKQAGLLLPFLDKKHGGRKGSQFEIQEVLRIAGHYGVPVTLRTGIEGALVLQPLQ
                     EFGDEAQIAQGLDMVFKGEGGGLGVTEPETSGAAIAREMQSYYEYTDGQTIYVNAAKY
                     WQGNSQSDFLLVAAKERKNGKLAKVIDLLLVPKTYIRCETLASEGLRAVRYAVNRIDA
                     EMPATAVMKLSQSDAAGLRAFQNIFIRSRLQLIGMTHGIMEYILENLERYVRNDIRFV
                     DYERREIRRRHQVSEILYRYVCHSVSPVAPVAHQLMEANIVKTLATEYTYAAAQMLQK
                     LLGAKGFERGHTAGNIAIDIRPFTIFEGPNDMLYAEIYDQFVRATAEEKEAGMKLDKN
                     QTLLDRLQTDARFAAVARDYTLPEDIRSFLQEHTLTDACALQKVFIGKIIARLFVFVQ
                     AEHEDTAAFLLNDIRKDILDCRYCG"
     gene            20633..24733
                     /gene="mfd"
                     /locus_tag="WGQ68_000444"
     CDS             20633..24733
                     /gene="mfd"
                     /locus_tag="WGQ68_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEZ0714312.1"
                     /translation="MTYPIPKPREKSRWPNLSQGSLPLALARYLPHKRLKVVLTQDAE
                     QALRLQTAWRFFRPHDTAVFLPDWETLPYERFSPHQDLVSERLSALWQIKSGAADVLF
                     VPVATAMQKLPPVPFLAGRTFWLKTGQTLDIGRLKTDLVDAGYNHVSHVVAAGEFAVR
                     GGIVDLFPMGSEMPYRIDLFDDEIDSIKTFDTETQRTISPVSEIRLLPAHEFPTDSEA
                     QKIFRSRFREEVDGNPNDAAVYKAVSNGHFGAGVEYYLPLFFENELETLFDYIGEDAL
                     FVSLGDVHAEANRFWSDVKSRYAMAQGDETYPPLLPQHLYLSADVFAGRLKNYGQVLP
                     DVSGKEHALPDLAVNRQSDEPLQALKDFQTTFDGRILLCAESLGRRETMLGFLQQNGL
                     KAKPVSDWQGFLSAHEPLMITVAPLAYGFKLGGLQSPNQQQTTSASEGEGDAVTDQTE
                     FSASATNPLPSPLPQEREQSAAAVSDSLKAAAVSTESRLPPGKSNLHGQIQQQPTPSP
                     MGEGWGEGKAVAAQTEFSAAATNPLPNPLPQEREQSAAAVSDDLKAKSSLHLVASNLH
                     GQIRQQPTPSPVGEGWGEGKAAADQTEFFLAVTSPLPQEREQSAAAVSDGLKTESSLY
                     SIANNLHGQIRQQPAPSPVGEGWGEGKAVADQSAIAVITESDLYQYVARSRVHNRRKK
                     HAAVSDGLLRDLAEINIGDPVVHEEHGIGRYMGLITMDLGGETNEMMLLEYAGEAQLY
                     VPVSQLHLISRYSGQAHENVALHKLGSGAWNKAKRKAAEKARDTAAELLNLYAQRAAQ
                     SGHKFEINELDYQAFADGFGYEETEDQAAAIAAVIKDLTQAKPMDRLVCGDVGFGKTE
                     VALRAAFVAVMGGKQVAVLAPTTLLVEQHAQNFADRFADFPVKVASLSRFNNSKATKA
                     ALEGLADGTVDIVIGTHKLVQDDIKFKNLGLVIIDEEHRFGVRQKEQLKRLRANVDIL
                     TMTATPIPRTLSMALEGLRDFSLITTAPSRRLAVKTFVKPFSEGSVREAVLRELKRGG
                     QVFFLHNEVDTIENMRERLETLLPEARIGVAHGQLRERELEQVMRDFLQQRFNVLLCS
                     TIIETGIDIPNANTIIINRADKFGLAQLHQLRGRVGRSHHQAYAYLLTPEYITKDAEK
                     RLDAIAAADELGAGFTLAMQDLEIRGAGEILGEGQSGEMIQVGFTLYTEMLKQAVRDL
                     KKGRQPDLDAPLGITTEIKLHSPALLPESYCPDIHERLVLYKRLAVCETVQQINAIHE
                     ELVDRFGLPEQAVKTLIESHHLRLMAKELGIDAIDAAGEAVTVTFGKNNNVDPTEIIL
                     LIQNDKKYRLAGADKLRFTAEMENIEVRINTVKNVLKTLQNRCLPK"
     gene            24882..25265
                     /locus_tag="WGQ68_000445"
     CDS             24882..25265
                     /locus_tag="WGQ68_000445"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEZ0714313.1"
                     /translation="MFRTMLGGKIHRATVTEADLNYVGSITVDQDLLDAAGIYPNEKV
                     AIVNNNNGERFETYTIAGKRGSGVICLNGAAARLVQKGDIVIIMSYVQLSEPEIAAHE
                     PKVVLVDGNNKIRDIISYEPPHTVL"
     gene            25287..26129
                     /gene="kdsA"
                     /locus_tag="WGQ68_000446"
     CDS             25287..26129
                     /gene="kdsA"
                     /locus_tag="WGQ68_000446"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEZ0714314.1"
                     /translation="MDIKINGITVGNDAPFVLFGGINVLEDLDSTLQTCAHYVEVTRK
                     LGIPYIFKASFDKANRSSIHSYRGVGLEEGLKIFEKVKAEFGIPVITDVHEPHQCQPV
                     AEVCDVIQLPAFLARQTDLVVAMAKTGNVVNIKKPQFLSPSQMKNIVEKFHEAGNGKL
                     ILCERGSSFGYDNLVVDMLGFGVMKQTCGNLPVIFDVTHSLQTRDAGSAASGGRRAQA
                     LDLALAGMATRLAGLFLESHPDPKLAKCDGPSALPLHLLEDFLIRIKALDDLIKSQPI
                     LTIE"
     gene            26144..26584
                     /locus_tag="WGQ68_000447"
     CDS             26144..26584
                     /locus_tag="WGQ68_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HEZ0714315.1"
                     /translation="MMQTFRKISLYAATLWLGMQIMAGYIVAPVLFKMLPKMQAGEIA
                     GVLFDILSWSGLAVWGAVLAAAFAALTRRQTALLLFLLSALAANRFLITPVIEALKYG
                     HENWLLSFVGGSFGMWHGISSIVFMATALLSAVLSWRLSGKDAV"
     gene            26631..27917
                     /gene="eno"
                     /locus_tag="WGQ68_000448"
     CDS             26631..27917
                     /gene="eno"
                     /locus_tag="WGQ68_000448"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEZ0714316.1"
                     /translation="MSAIVDIFAREILDSRGNPTVECDVLLESGVMGRAAVPSGASTG
                     QKEALELRDGDKSRYSGKGVLKAVEHVNNQIAQALIGIDANEQSYIDQIMIELDGTEN
                     KGNLGANATLAVSMAVARAAAEDSGLPLYRYLGGAGPMSLPVPMMNVINGGEHANNSL
                     NIQEFMIMPVGAKSFREALRCGAEIFHALKKLCDSKGFPTTVGDEGGFAPNLNSHKEA
                     LQLMVEATEAAGYKAGEDVLFALDCASSEFYKDGKYHLEAEGRSYTNAEFAEYLESLV
                     NEFPIISIEDGMDENDWEGWKLLTEKLGGKVQLVGDDLFVTNPKILAEGIEKGVANAL
                     LVKVNQIGTLSETLKAVDLAKRNRYASVMSHRSGETEDSTIADLAVATNCMQIKTGSL
                     SRSDRMAKYNQLLRIEEELAEAADYPGKAAFYQLGK"
     gene            27932..28210
                     /gene="ftsB"
                     /locus_tag="WGQ68_000449"
     CDS             27932..28210
                     /gene="ftsB"
                     /locus_tag="WGQ68_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HEZ0714317.1"
                     /translation="MKWVTVVLSFALVCCQYSLWFGKGSIGRNSSLREQIAVQEEKNQ
                     TLALRNHSLAAEVYDLENGQEAISEIARVELGYIQDGETFYRLIRHNR"
     gene            complement(28251..28541)
                     /locus_tag="WGQ68_000450"
     CDS             complement(28251..28541)
                     /locus_tag="WGQ68_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S ferredoxin-like protein"
                     /protein_id="HEZ0714318.1"
                     /translation="MARIGTNKGFFELLEGETLLEGLERTGHMVEYQCRSGYCGSCRV
                     KILEGSVTYREPPLAFLGRDEILPCCCYVEGDVRLDCGLSGEDEGLSDSLLK"
     gene            complement(28662..29795)
                     /gene="nrdB"
                     /locus_tag="WGQ68_000451"
     CDS             complement(28662..29795)
                     /gene="nrdB"
                     /locus_tag="WGQ68_000451"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HEZ0714319.1"
                     /translation="MSYSTFPKTKNDALNEPMFFGQPVNVARYDQQKYEVFEKLIEKQ
                     LSFFWRPEEIDVSRDRIDYANLPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLVSI
                     PELETWIETWSFSETIHSRSYTHIIRNIVNDPSVVFDDIVENEYITARAEDIACYYDD
                     LIEYTQYYNLLGEGMHNVGGKPVTVSLRGLKKKLYLCLMCVNVLEAIRFYVSFACSFA
                     FAERELMEGNAKIIKLIARDEALHLTGTQHMLNLMRSGVDDSEMAEIAAELQDECFQL
                     FKKAAEQEKEWAAYLFKDGSMIGLNKEILSQYVEYITNLRMQAVGLPAGFEGANQNPI
                     PWINAWLSSDNVQVAPQEVEISSYLIGQIDSEVNTDDLGDFEL"
     gene            complement(29856..31043)
                     /locus_tag="WGQ68_000452"
     CDS             complement(29856..31043)
                     /locus_tag="WGQ68_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction enzyme"
                     /protein_id="HEZ0714320.1"
                     /translation="MNDLAAQTIQVVNKSDDLVASAIQTANKSIAVYCRYIRPNDVGT
                     TGSHQSGFYIQKHFSDDLFDVVCQKGTNKTISIKINWQDGSVTNSNFKYYGQGTRNEA
                     RITGFGKNFEFLSDKYSGSLLVLCRACYKDLLFHAFVLSSDEDIEIFIAETNILPGSL
                     YLPKKQEVEDNLTKLFSLFPNFPKTEEMAVLARENIALNKTNVSNVLKQWVSKEYELF
                     SIFEKREFEIIKSKITDLDSFINFAHSFTNRRKARAGKSLELHLSRIFDEFSLKFETQ
                     AKTEGKKKPDFLFPGSEEYHAMDESGNFIFQTEKLTMLGSKTTCKDRWRQVLNEADRI
                     PHKHLFTLQEGISDTQIQEMSDENLTLVVPKESVKTFGTFGKTHVLTLENFIKYIKSQ
                     QVS"
     gene            complement(31036..32049)
                     /gene="dcm"
                     /locus_tag="WGQ68_000453"
     CDS             complement(31036..32049)
                     /gene="dcm"
                     /locus_tag="WGQ68_000453"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0714321.1"
                     /translation="MKNKIQNQLDNIVTLNNEKFTFIDLFAGIGGFRIAMENVGGRCV
                     FSSEWDDKARQTYQVNFNDIPYGDITLKETKAAIPSKFDVLTAGFPCQPFSIAGVSKK
                     KSLGRETGFLDKAQGTLFFDVAEIIGKHRPKIFLLENVKNLVSHDKGNTFKVIKGTLE
                     ELDYQIFYQVMNAKYYVPQNRERIFIVGFDRQYFNKEINFNFPSPPESQPKLKQILED
                     DVDNSFTLSDNLWLYLQNYAKKHKAKGNGFGFGLVDLDGISRTLSARYYKDGSEILIP
                     QKGKNPRKLTPRECSRLMGFPKDFVIDAVSKTAAYKQFGNSIAVPLVQAIAKQIINEL
                     KNE"
     gene            complement(32107..34386)
                     /gene="nrdA"
                     /locus_tag="WGQ68_000454"
     CDS             complement(32107..34386)
                     /gene="nrdA"
                     /locus_tag="WGQ68_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HEZ0714322.1"
                     /translation="MNTPTDLKVTKRDGRLEAIDLDKIHRVVTWAAEGLENVSVSQVE
                     LKSHIQFYNGIRTDDIHETIIKAAADLISEDTPDYQYLAARLAIFHLRKIAYGEYEPP
                     HLYDHVKKLTDAGKYDRHILEDYSREEFDELNAYIDHERDMSFSYAAVKQLEGKYLVQ
                     NRVTRQIYETPQFLYVLVAMCLFSKYPKETRLDYVKRFYDAVSTFKVSLPTPIMSGVR
                     TPTRQFSSCVLIECDDSLDSINATTSAIVKYVSQRAGIGINAGRIRGLDSEIRGGEAR
                     HTGCIPFFKMFQAAVKSCSQGGVRGGAATLFYPLWHIEAESLLVLKNNRGVEDNRIRQ
                     LDYGVQINRLLYTRLIKGGNITLFSPNEVPGLYEAFFADQDEFERLYTKYEQDPNIRK
                     RIIPAADLFSTLMQERAGTGRIYIQNVDHCNTHSPFDPCVAPVHQSNLCMEIALPTKP
                     LDNINDPNGEIALCTLSAFNLGALNSLDELEGLADLTVRALDALLDYQGYPVEAARTS
                     TMGRRSLGIGVINYAYYLAKNGVRYSDGSALGLTHRTFEAIQYYLLKASANLAKEYGA
                     CTLFNQTVYSQGKLPIDTYKKDLDAVCGEPLHYDWESLRADIVKYGLRNSTLTALMPS
                     ETSSQIANATNGIEPPRGLVTVKASKDGILKQVVPEFETLKNAYETLWQLPGNEGYLK
                     LVGVMQKFVDQSISANTAYDPGKFEGGKVSMKQMLKDLLTAYKYGVKTLYYHNTRDGA
                     DDTQTDIQDDGCAGGACKI"
     gene            34915..35216
                     /locus_tag="WGQ68_000455"
                     /pseudo
     CDS             34915..35216
                     /locus_tag="WGQ68_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229534.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein"
BASE COUNT         8578 a         9792 c         8472 g         8388 t
ORIGIN      
        1 gcattttaaa atcctcggtt tgaatttgtc aatatcaact gtctgtttta aaatattttt
       61 ttaatttaaa cggcgttttt tgggaaacgg gcgacgccgt ctgaacgtct gtctgcgtgt
      121 tactgcccga caacaacgcg acggattttg acgggctgta cgggtacgtt ttgataaaag
      181 ccgcgcgtgg cggttttgac gcgggcgatt ttggaaacgg tgtccattcc gctttcgacc
      241 ctgccgaaaa cggtatagcc gtattgtccg tttttgtagt tgagtgaatc attgtccgcc
      301 agattgataa agaattggct ggtggcggaa tcggggtctg tcgtccgcgc catggcgatg
      361 gtgccgacag tgtttttcaa gccgttgccg gattcgttgg caacggcctt atcgcttgcc
      421 ttttgtgcca agtcctcggt caatccgccg ccttggataa cgaagccgcc gatgacgcgg
      481 tgaaaaatcg tattgtcgta aaagcctttt cgggcatagc gcacgaaatt ggcaacggtt
      541 ttgggtgctt tggattcgtc caaaaccaaa cggatattgc ccatatcggt ttccatcaaa
      601 acatgggttg ccgccataga cggcagggaa accgccaaaa gcagcgcggt taaaacggtt
      661 ttgaatttgg gtttcatccc gtcctcctca gaccttcaga cggcattttc atttcatatg
      721 ccgtctgaag gcgcgtgaac gctattccaa tgcgtctttg agcttttgtt cgattaaatc
      781 cgcatcaaac gatttggcaa tcaattcaaa acgcgaatcg cgccgccatg agacttggtt
      841 cgcgccccac tgtccgtcta cccagttgag ccacacccac gttcccaata cttgaaacac
      901 gcctttggcg cggacgagtc cttcggtcat tttgggcagg tcgttgaaaa agttggtcag
      961 cttttcgccg tcgaaatcgc gtccggcggg gaatgtgaaa ccttgcgact ggaagcccat
     1021 ggtgttgtcc ggcagggctt tgaggcggta gcgtgatttt tcgatgacgg gaatatcgag
     1081 ccaccggatg tcgagttgtg cgttttggac ttcgaccact ttggctttgg gcgggaacag
     1141 ttttgccgct ttgtcgtgaa attcggcaag ctgttcggtg gtgcataaat cggttttgct
     1201 ggcgaccaat acgtcgcaga tgccgatttg gtctttatac aacgcctgtt gcgcgtaatc
     1261 ggggttgatg aactggcgcg gattgacgac ggtaaagacg gcgccgattt ccaaaaggct
     1321 gtccagcggt ttggctttga gttcgtcgat gacgctggct gcgtgagcca gtccgcttgc
     1381 ttcaatcatc aggcggtcgg gcttggcgtc gcgcagcatt ttctgcacgg ttacgcccat
     1441 ttgcgggccg gcggtgcaac acaaacagcc gccggcgatt tctgccacag ggatgccgtt
     1501 gtcgctcaat accgcgccgt caataccgat ttcgccgaac tcgttgacga tgatgaccca
     1561 tttttcgttc gggtcttttt gcgccatcag gcttttgagc gcggtggttt tgcctgttcc
     1621 cagaaaacct gaaatcaggt ggactttggt ttttttcatt tctgacattt tttacaaatc
     1681 ccagttaaaa caacgtgttc ttctttcagc gcaaagccgc tttcggcaac gcctgcgcgc
     1741 agtgccgccc actcgtggct gagggtttgc tcgtccgccg tgccgcattc ggtgcagacc
     1801 aaaataaacg cgctgtggtg cgcttcggct tcttcgtggt cgtggcaatg gtcgtcgcac
     1861 tcgtgctgcg cgtggctgca caaaatatag ccgttgaccg ccgccacttt gtgcaaaacg
     1921 ccctgctccg cccaaaaatc aagggcgcgg taggcggtag gcggtgcaag cacgccctcg
     1981 ctttgctgct gcatctgcga caagacgttg taggctttaa tcacgccgct ttgctgcaag
     2041 acaatatcta atacctgctc gcgcaaagcg gttacctgca agccttcgct gcgtgcctgt
     2101 tcgataattt tctgtttgaa atttgttttc ataaattctc tgtttatgcc gtctgaacaa
     2161 ccgatacggc aggaggcggt tttatatttg tattcaattg ctttatttgg aaatcttttc
     2221 caacaatgcc cgacagcccg catccgcaag cctgaaggtt tcttcaaaat cgcccgtgta
     2281 ccacggatcg gggacatggt cgtaaccgct ttcgggtatc aggtcggtca gcttgaatat
     2341 tttttccggc cgcctgccga aggttctttc caattcggac aaattcttgc cgtccatcgc
     2401 gatgatgcag tcaaacgccg ccgcatcgct ttggcggatt ttgcggctgg taaagcctga
     2461 agcatcgata ccgtgttttt tcaatatctt tgccgtctcg cggtgcatat cttccccgtc
     2521 gtgccagccc gatgtccccg cgctgtccgc ttcaagggga atgcccgctt cggcggcgcg
     2581 gcggcgcaaa atgtattccg ccatcggcga acggcagatg ttgccgaggc agacaaaaag
     2641 gattttcggt tttttcatat cccctccctg ttccggcgcg atgccgtctg aagcggaaac
     2701 cctttcagac gtcatcggtc ggttaatcgt aaccgcataa ggcagaacca gccaaaacat
     2761 ccatctggaa gaaatggttt ggctaaatct taggcatatt taataagtgt ccaatattag
     2821 aagccgtatg ctccaaatag agactggcat ttttcaaact atcttctaaa ggttcacttt
     2881 tctccaaaat agaaaaggca gcttggatat tttcaaatgg cagggaaggc aaatcttcaa
     2941 cgagactgcc acaaatagcg acaacaggaa ctccgacagg ggttcttttt gctacaccaa
     3001 taggcgcttt ccctgctaaa ctttgacgat ctagtcttcc ttcaccaatg ataaccaagt
     3061 caacatctga cactttctta tcaaagtcaa tcaagtccag gcaggtgtca attccagata
     3121 cgatacttgc ctgagcaaag gcgcacaaac caccagcgat gcctccacca gctcctgctc
     3181 ctttaagttt taatgttgca ggggagactt tttcataaaa atcttgtatt gcctgatcta
     3241 cggcctcaaa catagtagaa tccaaccctt tttgcttgcc aaacgtatag gtcgcacctt
     3301 ggtgtccaca taagggactc acaacatctg ctaaaatacg aatgtgaaca tcttcaggaa
     3361 tttcatagcg attttctgtt gaaacagaag ctaggtttag taaggcttga ccgcaagtgg
     3421 gcaaggtatt tccatcctca tcataaaatt gataacctaa accagcagca atcccaatac
     3481 ctccatcatt actggccgta ccgccaacgc caatatagat ttctttaatt tcttgactaa
     3541 tgaggtggcg aatcaattct ccaataccac gagtttggat ttgtaatgga tttcgtttct
     3601 ctagcgggat ttttccaaga ccaaccaaat cagcaacttc gaatagggct agttgttctt
     3661 tttgaaaata gcgcatgact tctttttgtc caaaaggtcc tgtcacttgg agacattttt
     3721 cttctaggtc aagagaatgt cggatagcat ctacagtgcc ttctccccca tcaccgacag
     3781 gacagaggag acattccaca tctgctatcg cttgttggaa gcctcttttt attgcttcag
     3841 ctacctgttg agctgtcaag ctttccttaa acgaatccgg tgcaattaca atcttcatat
     3901 ttataattca tcctttcgtt tcactcaagg cacaacacag aatgaaaaag tgttgtgctc
     3961 tttattttga tttattatag aaatgagaaa gcctatcact actacaaatc actatgcgct
     4021 gaaaaacgga ttgtgccctt cccgtttcaa tgcttccgca tagctcggga tgctttcctg
     4081 ttcgcccaag ggattgtgca acaggtaaac gtgttctaca cggcattctg ccattaaatc
     4141 caaaaaattt tgctgaacaa cggcgatatt gtcggtaaag gaaagctgcc acgaaaacac
     4201 ctgcggcacg acttcaaacg cgccgtcggt cgcattaaat ccgaatagaa tctgtccacc
     4261 tacctttgcc gcagcgacca cgccgacgcg cgggctctgc atacggacgg cgcgtatcgc
     4321 gtttgccgca aaaacatcca tcgccatacg ttggcgcgtg tggctgccct ctgaaagcgc
     4381 gcgtaccgga gaatccgggg aaagcgggtt ggcaaacagc gtaacgcctt ccgatgcaag
     4441 attttcctgc catacctgca tcaagggcag acctgcctga agaaggttct gacccaatgc
     4501 cgtctgaact ttgccgctac cgaaacccag cgcgtaaacg acgtggacgg actgcccttc
     4561 gggcaacagc aaagggcgcg gggcgaaacg gcgtaagtgt tgcgctgccg cttcggtatt
     4621 ttgtttggca cgccaccact catccggcgc gaccgcactt aaatcggaag aatgcacaag
     4681 ataaggcagc cattgtgtct cttgcgccaa cgcgcgcagg tgcggatagt tttgcaaaca
     4741 ggcaaacaat gcctccgtcg gcagcttcat ctcgattgcc cgttctttct tgcagccgga
     4801 caccagcacg accggcaggg cgaaccactg cacttcgcct tctttctcgc aatcgagtac
     4861 cgcgttcaca ctggaaagca gcgcggcata agttccggca tcgggcgaca tcgtcagcgc
     4921 gagggaaagg ttgatatagt ggttttgttc aagcatcccc ctgatttcgg tttgaagctg
     4981 cccggacgag agtttgcgcg aagcctggga agaattgtgc gccaactggt aggcattgag
     5041 cagcaggtgg ttcttaatcg gattttgggg atacgggcgc gtatcgggca aggtaaatgt
     5101 ctggttcata tatgtcgtgt accgcttttc gcagtgtgtg tttttcctgt ttgaaaatat
     5161 atcggacgga ttgccgtcgg gacttgcccg tcaatccgcc gaaacgagaa aatgcctgtc
     5221 tgccaagtct gccaatattt cttccacata cacttcggca ggcggatgga atgtcaaacc
     5281 gtcgggcgtg gtgctgacga tgttcaagtt ttcagcaacc gcatctatca ccgcctgttt
     5341 gctcgtcggc cgcgacaatt gccgcataat gtacaaatgc ccgtatccga atgagggggt
     5401 gctttcgttg taacggttgg caggccggac aaacgcttcc gcgccgtgtt ccacaaaggc
     5461 ggcggcataa tctgtaaaac gcgccggcac ataggtttta tggtcttcaa aaaatggttt
     5521 gcccgcattg tctgatgaaa cagaaaaacg cccgagaatg gtttgcaata aaagctggga
     5581 attgattttc atacggtcaa attccagacc gggaaatcgg cgggcgaggt tttccgcaac
     5641 atcttccgtt ttaaacgcct ctccggtttt catcacatcg cgtatgcccg aaagcagggt
     5701 atcattttca tcaaagttga ttgtttcccc tcttgccggg cggaaattca aactttctat
     5761 cacttcgacg gcaaccgact catcgcgcct gatattgtcc ccgacttcct cacggcataa
     5821 aagcgaacgg cggaattggc ggtcggataa aatatcgcta taaaattctt tggcaatata
     5881 atcgccccct gccaatgcca gaatccgctc ccgcgtatgc tccgccatcc aagaaacaaa
     5941 agacgcgtgc aaatcggtat ccccgatata tgcgagcctg tggcgattag cccattcgat
     6001 gaagccgttg acgtaaatcg ggtcgttaaa cgcctccata tattcgtgtg cgatgtaata
     6061 aaaattatga ttcaatattt tttgaatcgc cggaagtttg ccgccgccgt ccaagccctt
     6121 gtcgttttcc aaaatttccg ccagcgcctt gagcgcgtcc aagcctttcc gcgtccgcgc
     6181 ttccaagggt tcttcaagca catccctgcc ggcaaagtac ataatttcgc gcaactgctc
     6241 ctgccgtttc cagcccgggt aaacattgta tgaaatatag gcaatgccgt gttcggtcag
     6301 gttgttccgg caaatcgaaa aaattttgtc tttaactgcg tcaggcaccc acgaccaaat
     6361 gccgtggacg atgatatagt caaacttccc gaatgactca tcgatggtca aaatatcttt
     6421 ttcttccaga cgcacatttt tcaagcccat tttttcaatg atggcgttgc cctgcgcaac
     6481 ctgcctgccg gacaagtcga taccgacaaa ttccgcatcc gggtaataaa gagcctgcgt
     6541 gatgatattg ccgcccatcg aacagcccag ctccaagacc ttggcttttg ccgctgctgc
     6601 aggcttcagg cccatcatgc gggcgcgagc ctccaaggcg ttgatggcgg tttgggaaaa
     6661 tgctttggat tcatacatca aatcatcata tgaatttttg atgttggaca cgtccggcac
     6721 accgttctcc gttgcagcac gcgcaaaggc ggcttttttc tttttgttcg gcatattttg
     6781 tttgtctgaa ggcactattg cccgcaagtt taaccaattc atcctacccg ttcaactaaa
     6841 tcaaatgccg tctgaaggcg cggagcgtac ttcagacggc atcggggagg cgcgaaggct
     6901 tcagacggca tttttgccgc tttatttcaa cgcggcatca tagccgccgt cttcaacggc
     6961 ttcaatcaac gcccctgcat cggtttgcgc ggggtcgtat ccgacggtcg cacttttgtt
     7021 ttcaagactg acttcgacgc ttgccacgcc ttttacgcct tccaatatcc gggtaacgct
     7081 tttgacgcag ccgccgcagc tcatgccgcc gatgtcgagg ataagggttt ccatcatttt
     7141 cctttcgttg gaactgcaat ctgacgggcg ttattgtaag tcggggcgtg aacttgggca
     7201 aacgcggaaa cggtgcggcg gtttggaaaa agacggacgc ttgcgcataa tggcggcaat
     7261 tcccatcagg acgacaacaa tgaacgcttc gcaaaaaccc tggttgagca tcatcgcctt
     7321 ggcaatcggc gcatttatag tggattaaca aaaaccagta cgtcgttgcc tcgccttagc
     7381 tcaaagagaa cgattctcta aagtgctgaa gcaccaagtg aatcggttcc gtactatttg
     7441 tactgtctgc ggcttcgttg ccttgtcctg atttttgtta atccactata caacgtcggt
     7501 atcggcagcg gcgcactgtt gggacattgg gtaacgcaat atgtcggcat ttcccgtatc
     7561 ggcatcgccg gtatgcttgc ggcggcggcc gggctgtggc tgtgcctgaa cttgaaccgc
     7621 catatccaaa cctgaccgcg cgatgccgtc tgaagcccct cccgcccttc agacggcatt
     7681 ttgattgaag aaatatccgc ccctgcttaa aataacgggc tttgccgtgt tttaacgcct
     7741 attttcttat tccaaggatg gtttatgccg ctgctgtctg tcgagttcgc actgttcttt
     7801 ctcgccttcc tgccgattta ctggggcttg gcgaaatacc cgtccgtcca aaacctgctg
     7861 cttttggctg ccggtatggg ctggctctac catatcggcc ccgtatttgc ggcaatcgtc
     7921 gtcctttatt cctcctgcgt gtaccttttg ggcgaactgc tccgttccga tcgcgaaaat
     7981 acgcgccgct tttggctggg gtgcggcatt gccgtctcgc tgaccgtctt gggctttttc
     8041 aaatatttcg actttttccg cccgctgatt gcacaatatg ccggacaagg cggcgcaatc
     8101 gacatcctga tgccgctggg gctttcgtat tacaccttcc agtcgctcgc ctatctggtt
     8161 tactgcttcc gcgccccgca cgccgcgcgt ttcgagtggc acgagctgct gctgcacctg
     8221 agttttttcc ccaccgttac ctccggcccg attatccgcg ccgccgcatt caaaagcgca
     8281 gacggcgagc aggcgggcgc attggcgcaa atccgtaccc gccaaccgcg ctcacccgtc
     8341 cgccccgcac tcgccgtttc cctgattctg ctgggtattg ccaaaaaatg gtggctggcg
     8401 gggatgctgg cggaaaactg ggtgtcgccc gtatttgaaa atcccgccca attcgacggc
     8461 tggggcgtat tgggcggcgt gtacggctat accttccaac tctttttaga cttttccgga
     8521 tattccgatt tggttatcgg catggcgatg ctgctgggtt tccggctgcc caaaaacttc
     8581 tccgcaccgc ttcgtgcttt aaacatccgc gcattttggg acaaatggca catcagcctt
     8641 tccacctgga tacgcgacta catctacatc cccttgggcg gcagcaaaaa aggcttttta
     8701 cggacacagc tcaacctgat ggcggcaatg gtactctcag gcatctggca cggctacggc
     8761 tggaacttcc tcatttgggg cgcgctgcac ggcacggcac tggtgctgct caacacgggc
     8821 gatcgctatt tcggacgcaa cgcgctatgc cgtctgaaat accttgcgcc gctctcgtgg
     8881 tttgttacct tccatttcgt ctgccttagc tttgttgtct tcaataccgc aagccccgac
     8941 gatgcaggcg cagttttcaa tgccctcttt gccaatgcca acggctggaa tgcgccgcaa
     9001 caggcaaaca tgctgctgct tgcctcgttt gcatccgtga tgctgctcta cccttacctg
     9061 caacgcgctt tcgacggcgc ggtcaaaggt ttggaaaaaa tcccgatgtg gctgtggttt
     9121 atccccgttt ccgccgtcct gctgctgatt atcgtattgg ccccctcggg gattcccggc
     9181 tttatttatg ccaattttta agggtttgga catgaaaaac tttctttccc ttttcacttc
     9241 catattgatg tctgccctga ttgccgtgtg gttcagccaa aaccccatca acgcctactg
     9301 gcagcagacc taccaccgca acagcccgct cgaaccgctt gccgcctacg gatggtggcg
     9361 gagcggtgcg gcattgcaag aaaacgccta caccctttca gacggcatca aagccttcct
     9421 gtccggcgaa acgccgccga cggctcaaga cggcggctcg gcagatatgc cgtctgaagc
     9481 cgccacatcc gaagccgtcc ctcaaaccgg tgaaacagaa tggaaacagg acaccgacac
     9541 cgccgccgtc cgcagcggcg acaaagtctt ttttgtcggc gactcgctga tgcagggcgt
     9601 tgcccccttc gtgcaaaaaa gcctgaaaca gcaatacggc atcgaatccg tcaacctcag
     9661 caaacaaagc acggggctgt cctacccctc attcttcgac tggccgaaaa cgattgaaga
     9721 aaccctgcaa aaacatcccg aaatcagcgt gctcgccgtc ttcctcggcc cgaacgaccc
     9781 gtgggatttc cccgtcggca aacgctacct caaattcgct tccgacgaat gggcgcaaga
     9841 atacctgaaa cgcgtcgacc gcatccttga agccgcacac acgcaccgcg tccaagtcgt
     9901 ttggctcggc atcccctaca tgaaaaaagc caagctcgac gggcagatgc gctacctcga
     9961 caaactgctt tcggaacact tggaaggcaa aatcatcctg attcccaccg cgcacaccct
    10021 gagcggcggg gaagaccgcc acaccgactc cgtcaacgtc aacggcaaac ccgtccgcta
    10081 ccgcagcaag gacggcatac actttaccgc cgaaggacaa aaactgctgg cggcaaaaat
    10141 aatggaaaaa atcgtttttg aaccaagtac gcaaccatca agtacacagc catgaacccc
    10201 aaacacctca tcgcattttc cgccctgttc gccgccacgc aggcagaagc cctgcccgtc
    10261 gcctccgtca gcctcgacac cgttaccgtt tccccatccg ctccctacac cgatacaaac
    10321 gggctcttga ccgactacgg caacgccgcc gcctcgcctt ggatgaaaaa actccgatcc
    10381 gtcgcacaag gcagcggcga gaccttccgc atcctgcaaa tcggcgactc gcacaccgcc
    10441 ggcgacttct ttaccgacag cctgcgcaaa cgcctgcaaa aaacttgggg cgacggcggc
    10501 ataggctggg tttaccccgc caacgtcaaa gggcagcgta tggcggccgt ccgctataac
    10561 ggcaactggc aaagcctcaa cagtcgaagt gccgcagcag acttcccgct cggcggcatc
    10621 ctcgcccaca ccggcagcgg cggcagcatg accctgaccg catcggacgg caagaccggc
    10681 aaacagcgca tttccctgtt tgccaaaccc ctgctcgccg aacaaaccct gaccgtcaac
    10741 ggcaacaccg tctccgccaa cggcggcggc tggcaggtac tggatacccg cgcggcactg
    10801 cccttgacca tacagaccga aatgccgtgg gacatcggct tcatcaacat cgaaaatccc
    10861 gccggcggca ttaccgtttc cgccatgggc atcaacggcg cacaattgac ccattggtcg
    10921 aaatggcgtg ccgaccgtat gaacgacctt gcccaaaccg gcgccgattt ggttatcctt
    10981 tcctacggca ccaacgaagc cttcggcgac aacatcgaca ttgccgacac cgaacaaaaa
    11041 tggctggata ccgtccgcca aatccgcgac agcctgcctg ccgccggcat cctcatcatc
    11101 ggcgcacccg aatccctgaa aaacacgctc ggcgtatgcg gcacacgccc cgtccgcctg
    11161 accgaagtcc aacagatgca gcggcgcgtc gcccgacagg gacaaaccat gttctggtct
    11221 tggcaaaacg ccatgggcgg catatgcagc atgaaaaact ggctcaacca aggatgggcc
    11281 gccaaagacg gcgtacactt ctccgccaaa ggctaccggc gcgcggcgga aatgctcgcc
    11341 gacagcctcg aagaactcgt ccgcgccgcc gcgaaacggt aaacaaaaag gctttcagac
    11401 ggcatagccg ccccgcgcac gagttgccat gacaaggcgg attcgtccgc aaccggcata
    11461 cggcgcaaag gcggcgttcg atatgccgtc tgaaggcaaa gatgataaac tgccgccttc
    11521 cgttttcaga cggcatattg ttttcaaatg agggcgttct ccgtccgcaa ccataaagga
    11581 agtttcatga accggactta tgccaatttc tacgaaatgc tgaccgccgc ctgccgcaaa
    11641 aacggcaacg gtacggcagt gttcgacggc aaggaaaaaa ccacttacga tgcgctcaaa
    11701 caggaagtag aagccgttgc ggcgtacctg caaaatatcg gcgtgaagtt cggcgacacg
    11761 gtcgcgctgg cggtttccaa ttccacagaa tttattaccg cctatttcgc cgtctccgcc
    11821 atcggcgcgg ttgccgtgcc gatgaacaca tttttgaaaa acagcgaata cgcatatatt
    11881 ttgaacgact gcaaggcgcg gtttatgttc gcctcggccg gcctgtcaaa agaattggca
    11941 ggcttgaagg cgcaaacgcc cgtcgaaaaa atcatttgga caggcaaaag ccgacagggt
    12001 ggcggagcgg cggaaggcga tgcctttttt gaagacgtgc gccgtttccc cgaaaaaccc
    12061 gacttgggcc accaaccccg gataaatgat ttggcacaca tcatctacac ctccggcacg
    12121 acggggcatc ccaaaggcgc gctaatcagt tacgccaatc tgttcgccaa cctgaacggc
    12181 atcgaacgca tctttaaaat ctccaagcgc gaccgcttta tcgttttcct gccgatgttc
    12241 cacagcttca cgctgacggc tatggtgctg ctgccgattt atatggcgtg ttcgattatt
    12301 ttggtcaaat ctgttttccc gttttccaac gttttgaaac aggcactgct caaacgcgcg
    12361 accgtgtttt tgggcgtgcc cgcgatttac accgcgatga gcaaggcgaa aatcccttgg
    12421 tatttcagat ggttcaaccg catccgcctg tttatcagcg gcggtgcgcc tttggcggaa
    12481 caaaccatcc tcgatttcaa agccaagttc ccccgcgcca aattgctgga aggctacgga
    12541 ctgagcgaag cctcgcccgt cgtcgccgtc aatacgcccg agaggcaaaa agcccgcagc
    12601 gtcggcatcc ccctgcccgg tttggaagtc aaagccgtcg atgaagaatt ggtcgaagtg
    12661 ccgcgcggcg aagtgggcga actgattgtc aggggcggtt cggtgatgcg gggctacctc
    12721 aatatgcctg ccgccaccga tgaaaccatc gtcaacggct ggttgaaaac gggcgatttc
    12781 gttaccatag acgaggacgg cttcatcttt atcgtcgacc gcaaaaaaga tttgattatt
    12841 tccaaaggtc aaaacgtcta tccgcgcgag atcgaagagg aaatctacaa actcgatgcc
    12901 gtcgaagccg ccgccgtcat cggcgtgaaa gaccgttatg ccgacgagga aatcgtcgcc
    12961 ttcgtccaat tgaaggaagg tatggatttg ggcgagaacg aaatccgccg ccacctgcgt
    13021 accgtgctgg caaatttcaa aatccccaaa caaatccact ttaaagacgg gctgccgcgc
    13081 aacgctacgg gcaaggtatt gaaacgggtg ttgaaggagc agtttgacgg aaacaaatga
    13141 acgccgtgcc gtccgaagcc ccgtccggca aaaaaaatgc ggtgaatctg cttcaccgca
    13201 tttttttggc aaacgccgga gcgaggatat ttactctgcc aattccacct gctcgtgcgc
    13261 catcagttcc tgaccgacat catccaacaa catcaaataa cgttcgtagt ttttcaaaat
    13321 gtcggcaatc agctcgtcct tgttcatata ctgcacatcg tacccgacgc gcccgtcgaa
    13381 aaaataagcg tagggtttgt aagttgtctg gtgccggata tgcggcagct tgccgtcgtt
    13441 aatcaactgg tcggatacat cctgcccgac agacttaatc ccgtacataa aatcgcgcat
    13501 cgtctctttc cgaatgacga actcgattgc gggctcgtcc tgatgaaaca tcttatcgac
    13561 ccggacgctc aagccgtatt cttccgaaag ctcccgttgc aactcgtgca tagcgggcga
    13621 tgcggtatgt ttgaggaatt ttaaaatatc ctgctcctgc gtctggctca ttatctgcac
    13681 cagccgttct ttccacttgc cgcccgtcca aaatacactg gtagggttaa cccgggtctc
    13741 aaaatatttc ttatccgcac tcaatccttt ccacaggctg aaacacatta tcagcatcag
    13801 cagggcaaac ggcagggaaa caatcagggt catagactgc aggttgccga gtccgcccga
    13861 gcgcatcagc aaaacggcaa cggcagacat cagcacgccc cacataaccg cctgccaccg
    13921 tggcgcgctc aagcctttgt cccgagaggt aatattgttc aggacataaa tcccggaatc
    13981 ggcagaagtt acaaaaaaca gagaaatgac cagcaggctg acgatgctcg tcagttcggg
    14041 cagagggagg taattaaaga atttaaaaag cagcgtttcc ggagaggagg tcattttttc
    14101 gagcactccc cccgcaaccc cgtcattcag ccaaatcgcc gtattgccga agacggtaaa
    14161 ccacaaaacg ccgaacaggc cggggatgag caaaaccccg aagacaaact cgcggatggt
    14221 gcgccccttt gaaatgcgcg cgataaacaa acccacaaac ggcgcccaag aacaccacca
    14281 cgcccaataa agcaccgtcc aagattcaaa ccacggcttg tgttcccgtt cgtacgcata
    14341 agttttaaaa ctgaggcgca ccagatttcc gaggtagttc cctatgttgt cgccgaatgc
    14401 cgacaacagg taaacagtgg gacccgccgc caaaacaaaa aacagcagca aaaacgcaag
    14461 gctcaggttc aactcgctca acaccttcac gcccttcccc acgccggata ttgccgaaac
    14521 gacggcgagg gacatgacgg cggcgataat caaaacctgc acgctgaagc tgttttcggc
    14581 aatccagccc atttcctgca atccggcgcc cagttgcgaa gccccgaacc ccaatgtggt
    14641 gatgatgccg aaaaaagtag caagcaacgc cataatatca atggcatcgc cgaaccttcc
    14701 ggaaattttt tctttcaaca gggggtaaaa acaagaacgc agggcaagcg gcagcttgta
    14761 gcggaaaccg aaataagcca aagccaatgc aatcgtaccg tacaccgacc aagcgtgaac
    14821 gccccaatgg aacaccgtgt gcagcaatgc ctgctgctgc ctgtgttccg gcgtgccggc
    14881 cgtaatgtcc gaaaaataat gcatcaacgg ctctgccacg ccgaaaaaca tcagacccac
    14941 gcccatcccg gccgcaaaca gcatcgccag ccacgacagg aagccgaatt ccggcacatc
    15001 ttcatcccgt ccgagcctga tgtttcccaa actgctgacc gagagtatca gcaggaaacc
    15061 cagaaaaatg gaaaacgtta aaacataaaa ccagctgaac tcggtaaaaa tgacttcttt
    15121 tgcccgatcg agccacatct gcgcctgatc cggcacggtt aaaaccaata ccaccaaaac
    15181 acacacaaaa aacaaagtcg tcaaaataac catcggatta aatgacgttc ggcgttctat
    15241 aaattcagac agggacaaac cttctcactc ctttgttaaa aacagacaaa cccggtcatc
    15301 gggcaaagcg gtcaaaacct gccggtaaaa taccggcttc cggatgacga aatgcacaaa
    15361 ccggccgaaa ttgttataat cgaaaaaatt aaaaatcaat acggattatt ctatcagatt
    15421 gatttatcga tatttattat atcattaata tagtttgatt tcaaaccggc ggaaaatgcg
    15481 ccgcccaccg caggcgcgcc ctccccacca tcggggcgtt taacgccgga ttgccgatgc
    15541 ggtacaatgg ctgatatgaa gaaaattacc cctcaaaacc tacgccccct gctttcggaa
    15601 agcttgggac ataccgattt tgtcaacgtc ctcaacgcac tgattaaatt tttgcgccgt
    15661 ggcggcaaaa aatgtgcagg ggaacgtttc gacctgatta tcgacacatt caaacaagac
    15721 agggaattac tgtcccgctt cagccggtgt ttttacatct ggctcgcgca aatacacatt
    15781 tatccggcac tcatcaaact cggcatcttc tcgcgccaca gctttgcccg ggaaatgggc
    15841 atacgcatct acgaacgctt cagcccgtca tataaagatt ttgccaactt gggcgaagtc
    15901 ttcctttatc ttttccattc cgaaaacgac gacaaatggc tgcaaacgct caatatccgc
    15961 caatggctgg ttttatacga actcatccgg agccacgccg agccgtccaa attgcagacg
    16021 gcgggcatcc gccttgccga tgcgcgtttg cgcgccatcg aaatgctgtc tgtctggacg
    16081 gcatccgaag ccatcgaacc cgacctcatc cgcatcgccc cgcgcctgct ggaagccgat
    16141 tcttctttcg tcgccctcca acgcgaaacc gccaaactgg tcgaacacta ccgcaacggc
    16201 accacgcctt acgacaccgc ccacctcgaa gtgatgttcg accaatgttt cagccagatt
    16261 gactatttgc gccgcaaagg gacgggcgcc ggctctggtt cgtcggtcaa agtcgcccac
    16321 ctgctcgaac ggctccggca gaccgtagac cgtctgaagc tgctcaccga catccaaacc
    16381 ggtgtcggca acagcaaccg cctgaccatc gcgctgatga actccctcat ctacgcggcg
    16441 gtcgaacaat acagcacccg ccacctgcgc cgcagcagca tccgtatgct cgcccgcagc
    16501 attaccgaaa acaaaagcca ccacggcgaa cactacatca cccgcaaccg caaagaatat
    16561 ttcaaaatgt tctactcggc ggcaggcggc ggcgtcatca tcgccctgat ggcgctgctc
    16621 aaaatccgca tcggcacact cggcctcagc cccttcctca cttccttgtc ggcggggttc
    16681 aactacggca tcggctttat gatcatccat attctgcact gcaccgtcgc caccaaacag
    16741 cccgcgatga ctgccgccag cttcgccgaa caagttgatc tcaacgaagg cggcaaagcg
    16801 gtggacaaca agctcgccaa gctcctcatc gacgtatgcc gctcccaaag cgtcgccgtt
    16861 ttcggcaacg tttccatcgc catccttttg gcgtgcgcca tatcgttcgg ctatgcccat
    16921 ctgcaccaac agcccatact cgatgcccac accgccgcct accagttcaa atccatagac
    16981 atcatcgctt acccgacgct gtggtatgcc gccattgcag gtctgtggct gttctgctcc
    17041 ggcatcatcg caggtttttt cgacaaccgc gccgactacc tcaacctgcg ccaacgcctg
    17101 cccttcaacc ccttgctgcg taaaatcatg cgccccgggc cccgccgcgt cctcgccgcc
    17161 tacatccaca aacactacgg ctcgttggca ggcaacttca tcttcgggat gctcttgggt
    17221 atgaccggct atttcggaca cctcctcggg ctgccgctgg acattcgcca cgtcgccttc
    17281 tcctccgcca acctcggcta tgctgccgtc agcggcaacg tcggtttggg cacgttcgta
    17341 ctcggcattt tcagcgtcct cgccatcggc ttggtcaacc tctgcgtcag cttcagcctc
    17401 gccctcttcg tcgccctgcg ctcgcgcggc acgaaaatcg gcagtatccg caatctgatt
    17461 aagagttttt ggaatcagat taaaagcaat ccctgcatac ttttcctccc gcccgccaaa
    17521 gaacagggac atcctccttc ggacaagcct taaccggcaa tgccgtctga agcgggattc
    17581 gccccgaata ccgccctgat gcggaaaatc cccataaagg gatgcaaaaa tgccgtccga
    17641 accgcgtttc ggttcagacg gcattttata gtggattaac aaaaatcagg acaaggcgac
    17701 gaagccgtag acagtacaaa tagtacggca aggcgaggca acgccgtact ggttcaaatt
    17761 taatccacta taaaaaaaca ttacaatccc gggccgccga gcgaattggc aatgtccctg
    17821 accgccgtta catacatccg actgtgattg tactgccata ccgtataaaa attgttcaag
    17881 cccaaataat attcaaacac gccgggtgcg gtttccagtt tgaacaaaac cgccttttca
    17941 tcatcggcaa gctcttcgcc ggggatgatg ccgtacgcct tcaaatccgc caccgtccgc
    18001 gtcagggcgg ttttttcgcc aatgattgcc tgaacatccg cacccggcgc caatgttgca
    18061 gacaccagta ttttcccgcc cgtgcgccaa ccgtgctgct tcatataatt ggcaaccgat
    18121 gccgcgacat cgccgatgtt gccccatatg tcccgatgtc cgtctccgtc ataatccacc
    18181 gcccatttcc ggtagctcga aggcataaat tgcggcatcc ccattgcgcc cgcatagctg
    18241 cctttaaagg cgaaaacatc gccgccttct tcttttgcca gctttaaaag ctcgaccaat
    18301 tctttttgga aaaacccggc gcggcggggg taatcaaagc ctaaggtcgc caatgcgtcc
    18361 gccacacgga aactgcccgt atttttgccg taattcgttt caatcccgat aaccgccacg
    18421 ataagttcgg caggcacgcc gtatttttgc gccacatcat cgataagcgc gcggttttcc
    18481 gcataaaacc ggcgcgcgcc gcgaaatttc gccttgcccg aatttcccgt gcggaacaca
    18541 taccacggac gcgatgtgga gggtcggtgc ataatcttga cgatgtccgc cttgtaagcc
    18601 gctttgtcaa aaaaatcctg ccattccgcc cgggaaaaat cccctttccc gacttcatcg
    18661 tccacaaaac ggcggacatt tgcattggcg gcaaacccgc tgtcggatac cggtacggct
    18721 gccgcgtcaa acgcggggcg gctttctttt ttcatttcat ccgcgcgggg ggcttgggct
    18781 tcatttaccc ggggcgggcg tgcctccatc gccgtacagg cagacaaagc cgccaaacaa
    18841 attgccagcg gcagtatttt tctctttttc ataaatatgt tccgaacaaa tagggtaagt
    18901 gggaaagcgg cgcaaggggc gcggtgcgaa atgcggcatc cgccgcaatc ggcggctttg
    18961 tggaataccg cgcgttgcct tttgcaccgc ctgaaatccg tatgccgccg tatttgcccg
    19021 ctatccgcaa tatcggcagt ccaatatatc tttgcggatg tcgttcagca ggaaggctgc
    19081 ggtgtcttcg tgttccgcct gtacgaagac aaagagtcgg gcgatgattt tgccgatgaa
    19141 gactttttgc agggcgcagg cgtcggtcag ggtgtgttcc tgcaggaagc tgcggatgtc
    19201 ttcgggcaaa gtgtagtcgc gggcgacggc ggcaaagcgg gcatcggttt gcaggcggtc
    19261 gagcagggtt tggtttttgt ccaacttcat gcctgcttct ttttcttcgg cggtggcgcg
    19321 gacgaactgg tcgtaaattt cggcataaag catatcgttc gggccttcaa aaatcgtgaa
    19381 ggggcggatg tcgatagcga tattgccggc ggtgtgtccg cgttcaaaac ccttcgcgcc
    19441 caaaagtttt tgcaacattt gcgcggcggc gtaagtgtat tccgtggcga gggttttgac
    19501 gatgttcgcc tccatcagct gatgggcgac gggtgcaacg ggcgaaacgg aatggcagac
    19561 gtagcggtaa agaatctcgg aaacctgatg gcggcgccgg atttcgcggc gttcgtaatc
    19621 gacgaatctg atgtcgttgc ggacgtatcg ttccaggttt tcaaggatgt attccataat
    19681 gccgtgcgtc atgccgatca gttgcaggcg gctgcggata aagatgtttt ggaacgcgcg
    19741 caaaccggca gcgtcgctct gggagagttt catcacggcg gttgcaggca tttcggcatc
    19801 gatgcggttg acggcgtaac ggacggcgcg caagccttcg gatgcgaggg tttcgcagcg
    19861 gatgtatgtt ttggggacga gcagcaggtc gatgactttg gcgagtttgc cgtttttgcg
    19921 ttctttggcg gcaacgagga ggaagtcgct ttgcgagttg ccctgccagt attttgcggc
    19981 gttgacgtaa atggtttgtc cgtcggtata ttcgtagtaa gactgcattt ctcgggcaat
    20041 cgccgcgccg gaggtttcgg gttcggtaac gcccaaacca ccgccctcgc ctttgaaaac
    20101 catgtccaaa ccctgtgcga tttgcgcttc atcgccgaac tcttgcagtg gctgcaacac
    20161 cagcgcgcct tcgatgccgg tacgcagcgt aacgggcacg ccgtaatgcc ccgcaatccg
    20221 caagacttcc tgaatttcaa actggctgcc cttgcgcccg ccgtgttttt tgtcgaggaa
    20281 gggcaacagc aaacccgcct gcttcaaggc aagccatttg tcttcgggca ggtatcgcat
    20341 caggtcgata ccgtctgaaa aaatgcggcg gaatgcggat tcgatgtgct ttaaaaaagc
    20401 agccgtgtcc atagttgacg gctgcgcgct cggttcggtg tgtatcatcg gcttcctctg
    20461 tcggttccca ttaatcggcg gccggtcaaa ccgcctgcca cagtttagag ttgattttct
    20521 aaactttacc acaaagtgcg ccgggcaaca atccgccgac ctttcagaca gcatcgcgcc
    20581 ccctcccgtg ctaaaatgac cgtttgcatc actgtccgcc gattgccgca ctatgaccta
    20641 ccccatcccc aaaccccgtg aaaaatcccg ttggcccaat ctttcgcaag gctcgctgcc
    20701 cttggctttg gcgcgttatc tgccgcacaa gcggctcaag gtcgtgctga cccaagatgc
    20761 ggaacaggcg ttgcgccttc agacggcatg gcggtttttc cgtccgcacg acacggcggt
    20821 gttcttgccg gactgggaaa cgctgcctta cgagcgtttt tctccgcatc aggatttggt
    20881 gtcggagcgg ctctcggcgt tgtggcagat taagagcggc gcggcggacg tgttgttcgt
    20941 gccggttgcc acggcgatgc agaagctgcc gcccgtgccg tttctggcag ggcgcacgtt
    21001 ttggctgaaa acagggcaga ctttggatat aggccgtctg aaaaccgatt tggtagatgc
    21061 gggctacaac catgtttccc atgttgttgc ggcgggcgag tttgccgtgc gcggcggcat
    21121 tgtcgatttg ttccccatgg gcagcgaaat gccctatcgc atcgatttgt ttgacgatga
    21181 aatcgacagc atcaaaacct ttgacaccga aacgcaacgc accatttccc ccgtttccga
    21241 aatccgcctg ctgccggcac acgagttccc caccgacagc gaggcgcaaa agattttccg
    21301 cagccgcttc cgagaagaag tcgacggtaa tccgaacgat gcggctgtat acaaagccgt
    21361 cagcaacggc catttcggtg caggcgtaga atattatctg ccactgtttt ttgaaaacga
    21421 gctggaaacg ctgtttgact atatcggcga agatgcgctg tttgtctctt tgggcgatgt
    21481 tcacgccgag gcaaaccgtt tttggagcga cgttaaatcg cgttacgcca tggcgcaggg
    21541 cgacgaaacc tatccgcctt tgcttccaca gcatttgtat ctctctgccg acgtattcgc
    21601 aggccgtctg aaaaactacg gacaggtgct gcccgatgtt tccggcaagg aacacgccct
    21661 ccccgacctt gccgtcaacc gacaatccga cgagcctttg caggcattga aagattttca
    21721 gacgaccttt gacgggcgga ttttgctgtg cgccgaaagc ttgggacggc gcgaaaccat
    21781 gctcggtttc ttgcagcaaa acggtttgaa agccaaaccc gtgtccgact ggcagggctt
    21841 tttatcggca cacgagccgc tgatgattac agttgcgccg ttggcttacg gattcaaact
    21901 gggcggattg caatccccga accagcagca aactacttcc gcctccgaag gagaaggcga
    21961 tgcggttacc gaccaaaccg aattttccgc atccgcaaca aaccctctcc ctagccctct
    22021 cccacaggag agggaacaaa gtgcagccgc cgtttcagac agtctgaaag cagccgccgt
    22081 ttcaaccgaa agccgcctgc cccccggtaa aagtaatctg cacgggcaaa tccaacagca
    22141 acctactccc tcccccatgg gggagggttg gggagagggc aaagcagttg ccgctcaaac
    22201 tgaattttcc gcagccgcaa caaaccctct ccctaaccct ctcccgcagg agagggaaca
    22261 aagtgccgcc gccgtttcag acgacctgaa agccaaaagc agcctgcatc tcgtcgcaag
    22321 taatctgcac gggcaaatcc gacagcaacc tactccctcc cccgtggggg agggttgggg
    22381 agagggcaaa gcggctgccg accaaaccga atttttctta gccgtaacaa gtcctctccc
    22441 gcaggagagg gaacaaagtg ccgccgccgt ttcagacggc ctgaaaaccg aaagcagcct
    22501 gtattccatc gcaaataatc tgcacggaca aatccgacag caacctgccc cctcccccgt
    22561 gggggagggt tggggagagg gaaaagcagt tgccgaccaa agtgccatcg ccgtcattac
    22621 cgaatccgac ctctaccaat acgtcgcccg ttcgcgcgtc cacaaccgcc gcaagaaaca
    22681 cgccgccgtt tcagacggtc tgttgcgcga ccttgccgaa atcaatatcg gcgatcccgt
    22741 cgtgcacgaa gaacacggca tcgggcggta tatgggcttg atcacaatgg atttgggcgg
    22801 cgaaaccaac gaaatgatgt tgcttgaata tgcaggcgaa gcgcagcttt atgtgcctgt
    22861 ttcgcaactg catttaatca gccgctactc cggtcaggcg catgaaaacg tcgccctgca
    22921 caagctcggc agcggcgcgt ggaacaaagc gaaacgcaaa gccgccgaaa aagcgcgcga
    22981 taccgccgcc gagttgctca acctctacgc ccaacgcgcc gcccaatcgg gacacaagtt
    23041 tgaaatcaac gagttggatt atcaggcgtt tgccgacggc ttcggctacg aagaaaccga
    23101 agaccaggca gccgccatcg ccgccgtgat taaagatttg acgcaggcga agccgatgga
    23161 ccgactcgtg tgcggcgatg tcggttttgg caaaaccgaa gtcgccctgc gcgccgcgtt
    23221 tgtggcggtg atgggcggca aacaggtcgc cgtactcgcc ccaaccacgc ttctggtcga
    23281 gcagcacgcg caaaactttg ccgaccgttt cgccgatttc cccgtgaaag tcgccagtct
    23341 ttcgcgtttc aataacagca aagccaccaa agccgcactg gaaggcttgg cggacggcac
    23401 ggtcgatatc gttatcggca cgcacaaact ggtgcaggac gacatcaaat tcaaaaactt
    23461 aggtttagtg attatcgacg aagaacaccg cttcggtgtg cgccaaaaag agcagctcaa
    23521 acgcctgcgt gccaatgtcg acatcctcac catgaccgcc acgccgattc cgcgtacttt
    23581 aagtatggcg ttggaaggac tgcgcgactt ctcgctgatt accaccgcgc ccagccgccg
    23641 cctcgccgtc aaaacctttg tcaaaccctt tagcgaaggc agcgtgcgcg aagccgtgtt
    23701 gcgcgaactc aaacgcggag gacaggtatt tttcctgcac aatgaagtag atacgattga
    23761 aaatatgcgc gagcggctgg aaaccctgct gcccgaagcc cgcatcggcg tggcgcacgg
    23821 acaactgcgc gagcgcgagc tggaacaagt catgcgcgac tttttacagc aacgattcaa
    23881 cgtgttgctc tgttccacca tcatcgaaac cggtatcgac attcccaacg ccaacaccat
    23941 catcatcaac cgcgccgaca aattcggact ggcgcaactg caccagcttc gcgggcgcgt
    24001 cggacgcagc caccaccaag cctacgccta cctgctcacg cccgaataca tcaccaaaga
    24061 cgcagaaaaa cgcctcgatg ccattgcggc ggcagacgaa ctcggcgcag gttttaccct
    24121 agccatgcag gatttggaaa tccgtggtgc aggcgaaatc cttggcgaag gacaatccgg
    24181 cgaaatgata caggtcggct tcacgctcta caccgaaatg ctcaaacaag ccgtgcgcga
    24241 cctcaaaaaa ggccgccagc ccgacctcga cgcaccgttg ggcatcacca ccgaaatcaa
    24301 actgcacagc cccgccctgc tgcccgaaag ctactgcccc gacatccacg aacggctcgt
    24361 cctctacaaa cgcctcgccg tctgcgaaac cgtgcaacaa atcaacgcca tacacgaaga
    24421 actcgtcgac cgcttcggcc tgcccgaaca agccgtcaaa acccttatcg aaagccacca
    24481 cttacggctt atggcaaaag aattgggtat cgatgccatt gatgcggccg gcgaagcggt
    24541 aacggtaacc tttggtaaaa acaataatgt cgatccaacc gaaatcatcc tgctgattca
    24601 gaacgacaaa aaataccgcc ttgccggcgc cgataagctg cggtttaccg cagagatgga
    24661 aaatatcgag gtcagaatca acaccgtaaa aaacgtttta aaaaccttgc aaaacagatg
    24721 cctgcccaaa taaagccgac accgcaatgc cgtctgaaac accgttttcc ttgtccgaaa
    24781 gccgccatta tgaatttgaa ggaaactcca ctataatacg gcattcagat ttccagacgg
    24841 catcgcgccc gtcaaaccgc acacaaacca aaaggaaata catgttccgt actatgcttg
    24901 gcggaaaaat ccaccgcgcc accgttaccg aagccgattt aaactatgtc ggcagtatta
    24961 ccgtcgatca agatctgtta gacgcggcag gcatctaccc caacgaaaaa gtcgccatcg
    25021 tcaacaacaa caacggcgaa cgttttgaaa cctataccat tgcagggaaa cgcggcagcg
    25081 gcgtgatttg tctgaacggt gctgcggcaa ggctggtgca gaaaggcgat atcgtcatca
    25141 tcatgtctta cgtccaactc tccgaacccg aaatcgccgc acacgaaccc aaagtcgtct
    25201 tggtagacgg aaacaacaaa atccgcgaca tcatctccta tgagccgccg cacaccgtgc
    25261 tgtaaccctg aaaacggaca ttgaccatgg atattaaaat caacggcatt accgtcggta
    25321 acgacgctcc ttttgtactg ttcggcggca tcaacgtttt agaagatttg gattccaccc
    25381 tccaaacctg tgcgcattac gtcgaagtta cccgaaaact cggtattccc tatatcttta
    25441 aagcctcttt cgacaaggca aaccgttcct ccatccattc ttatcgcggc gtaggcttgg
    25501 aagaaggctt aaagattttt gaaaaagtca aagcagagtt cggcatcccc gtcattaccg
    25561 acgtacacga accccatcag tgccaacccg tcgccgaagt gtgcgatgtc atccagcttc
    25621 ccgcattcct tgcgcggcag accgatttag tggttgccat ggcaaaaact ggcaacgtcg
    25681 tcaacatcaa aaaacctcag ttcctcagcc cctctcaaat gaaaaacatt gtggaaaaat
    25741 tccacgaagc cggcaacggg aaactgattt tatgcgaacg cggcagcagc ttcggctacg
    25801 acaacctcgt tgtcgatatg ctcggtttcg gcgtgatgaa acagacttgc ggcaacctgc
    25861 cggttatttt cgacgttacc cattccctgc aaacccgcga tgccggttct gccgcatccg
    25921 gcggtcgtcg cgcacaggct ttggatttgg cacttgcagg catggcaacc cgccttgccg
    25981 gtctgttcct cgaatcgcac cccgatccga aactggcaaa atgcgacggc cccagcgcgc
    26041 tgccgctgca ccttttagaa gattttttaa tccgcatcaa agcattggac gatttaatca
    26101 aatcacaacc gattttaaca atcgagtaac acggtttcgc cttatgatgc agactttccg
    26161 aaaaatcagc ctgtatgccg caaccttgtg gctcggtatg cagattatgg cgggttatat
    26221 cgtcgcgccg gtgctgttca aaatgctgcc caaaatgcag gcgggcgaaa ttgccggcgt
    26281 attgttcgac atcctctctt ggagcgggct tgccgtttgg ggcgcggtac tggctgccgc
    26341 ctttgccgcc ctaacccggc ggcaaaccgc cctgctgctt tttttattgt ccgcccttgc
    26401 cgccaaccga ttcttgatta cacccgttat cgaggcactg aaatacggac atgaaaattg
    26461 gctgttgtcg tttgtaggcg gatccttcgg aatgtggcac ggcatttcca gtattgtttt
    26521 tatggcaacc gccctacttt cagcagtttt aagttggcgg ctttccggca aagatgccgt
    26581 ctgaagccct cccatttttt acctcccttc acttcacttg gagaacattc atgagcgcaa
    26641 tcgttgatat tttcgcccgc gaaattttgg actcacgcgg caaccccaca gtcgagtgtg
    26701 atgtattgct cgaatccggc gtaatgggac gcgcagccgt accgagcggc gcgtccaccg
    26761 gtcaaaaaga ggctttggaa cttcgcgacg gcgacaaatc ccgctattcc ggcaagggcg
    26821 tattgaaggc ggtcgaacac gtcaacaacc aaatcgccca agccctcatc ggtatcgatg
    26881 ccaacgagca atcttatatc gaccaaatca tgatcgaatt ggacggtact gaaaacaaag
    26941 gcaatttggg tgcgaatgcg actttggcgg tttctatggc ggttgcacgc gccgctgccg
    27001 aagactcagg cctgccgctt taccgctact tgggcggcgc aggcccgatg tccctgcccg
    27061 taccgatgat gaacgtcatc aacggcggcg aacacgccaa caacagcctg aacatccaag
    27121 agtttatgat tatgcccgtc ggcgcaaaat ctttccgcga agcattgcgc tgcggtgcgg
    27181 aaattttcca cgccttgaaa aaactgtgcg acagcaaagg cttcccaacc acagtcggcg
    27241 acgaaggcgg tttcgccccc aacctgaaca gccacaaaga agccctgcaa ctgatggttg
    27301 aagccaccga agccgccggc tacaaagcgg gcgaagacgt attattcgca ttggactgcg
    27361 cctccagcga gttctacaaa gacggcaaat accacttgga agccgaaggc cgctcctaca
    27421 ccaacgcgga atttgccgaa tatctggaaa gtctggtcaa cgagttcccc atcatctcca
    27481 tcgaagacgg catggatgaa aatgactggg aaggctggaa actgctgacc gaaaaactgg
    27541 gtggcaaagt ccaactcgtt ggcgacgacc tcttcgttac caacccgaaa atccttgccg
    27601 aaggcattga aaaaggcgtg gcaaacgcac tattggtcaa agtcaaccaa atcggtactt
    27661 tgagcgagac cctgaaagcc gtcgacttag ccaaacgcaa ccgctacgcc agcgtaatga
    27721 gccaccgctc cggcgaaacc gaagacagca ccattgccga cttggcagtc gccaccaact
    27781 gtatgcagat caaaaccggt tctttgagcc gttccgaccg catggcaaaa tacaaccaac
    27841 tgctgcgtat cgaggaagaa ttggcggaag ccgccgacta ccccggcaaa gccgcattct
    27901 accaactggg caaataagaa aaggttaagg tatgaagtgg gtaactgtcg ttttatcctt
    27961 cgcacttgtc tgttgccaat acagcctctg gttcggcaaa ggcagcatcg gacgcaacag
    28021 cagtctgaga gaacagattg ccgttcaaga agaaaaaaac cagacactcg ccctacgcaa
    28081 tcattccctt gccgccgaag tctatgattt ggaaaacggt caagaagcca tttcggaaat
    28141 cgcccgggta gaactgggtt atatccaaga cggtgaaacc ttttaccgac tcatcaggca
    28201 taaccggtaa taccgtcaaa aagccgtccg aaccaatgtt cggacggctt ttatttcaac
    28261 aaactgtcag acagcccctc atcctccccc gacaaaccgc aatccagcct gacatccccc
    28321 tcgacgtaac agcagcacgg cagtatctcg tcccgcccca aaaaagccaa aggcggctcc
    28381 cgataagtaa cgcttccctc caaaatcttc acccggcacg atccgcaata tccgcttcgg
    28441 cactgatatt ccaccatatg ccctgtccgt tccaagcctt ccaacagggt ctcgccctcc
    28501 aaaagctcaa aaaaaccctt attcgtacca atgcgcgcca tttccgacca atcaaaaata
    28561 aaaggaacaa taaccgatcc tacccgctgt ttttcccatc atacaacata caaatgccgt
    28621 ctgaaacatc cggcttcaga cggcattttt tcaaaaaaca tttacaactc aaaatcgccc
    28681 aaatcatccg tattcacttc agaatctatc tgaccgatca aataagagga tatttccact
    28741 tcctgcggcg cgacctgtac gttgtcggac gacagccacg cattaatcca cggaatcggg
    28801 ttttgatttg cgccttcaaa tccggccggc aaccccaccg cctgcatacg cagattggta
    28861 atatattcga cgtattggga taagatttct ttgttcaaac cgatcatcga accgtcctta
    28921 aacaaatatg ccgcccattc tttttcctgt tccgccgctt ttttgaagag ttggaaacat
    28981 tcgtcctgca actcggcggc aatttccgcc atttcagaat catcaacacc agaacgcatc
    29041 agattaagca tatgctgcgt gccggtcagg tgcagggctt cgtcgcgggc aatcagtttg
    29101 atgattttgg cgttgccttc catcaactcg cgctcggcaa aagcaaacga gcaggcgaat
    29161 gaaacgtaga aacggatggc ttccaacacg ttgacgcaca tcaggcagag atagagtttt
    29221 ttcttcaacc cgcgcaaaga tacggtaacg ggttttccgc cgacattgtg catcccttcg
    29281 cccaacaggt tgtaatactg ggtgtattcg attaagtcat cgtaatagca ggcaatgtct
    29341 tcggcgcggg cggtaatgta ttcgttttcg acaatatcat caaacacgac cgacggatca
    29401 ttcacaatat tgcggatgat gtgggtatag ctgcgcgagt gtatggtttc gctgaagctc
    29461 cacgtttcaa tccacgtttc caactcggga atcgaaacca aaggcagcaa ggcaacattc
    29521 ggactgcgcc cttggatgga atcgagcagt gtttggtatt tcagattgct gatgaaaata
    29581 tgtttttcgt gttcaggcag gttggcgtag tcgatacggt cgcgcgacac gtcgatttct
    29641 tccggccgcc aaaaaaacga caattgtttt tcaatcagtt tttcaaatac ctcgtatttc
    29701 tgctggtcat aacgggcgac attcacaggc tgaccaaaaa acatcggctc attcagcgcg
    29761 tcgtttttgg ttttgggaaa ggtgctgtat gacatagtta agtgttcgca ggacataatc
    29821 tattctctat attaattaaa aatgatctgt aagttttaac taacctgctg agactttata
    29881 tatttaataa aattttctaa agttaaaaca tgggttttcc caaaagttcc aaatgttttt
    29941 acggattctt tagggacaac taaggttaaa ttctcatcac tcatttcctg aatttgagta
    30001 tcagatatac cttcttgtag agtaaataaa tgcttatgag gaattcgatc tgcttcattt
    30061 aaaacttggc gccagcgatc cttacaagta gtctttgaac ctagcattgt cagcttttca
    30121 gtttgaaata tgaaattccc tgattcatcc atcgcatggt actcttcact tccaggaaat
    30181 aaaaaatctg gtttcttttt cccttctgtc ttcgcttgcg tctcaaattt taaggaaaat
    30241 tcgtcaaata ttcttgataa atgtaactct aaagattttc ctgctcttgc ttttctgcga
    30301 tttgtaaaag aatgtgcaaa attaatgaag ctatccaaat cagtaatttt tgatttaata
    30361 atttcaaatt cacgtttttc aaaaatggaa aataattcat attcctttga aacccattgt
    30421 tttaacacat tactaacgtt agttttattt aatgcaatat tttcccttgc caaaactgcc
    30481 atttcttcag ttttggggaa gttaggaaat agtgaaaata attttgttag gttatcctct
    30541 acttcttgtt ttttaggtaa atataaactt cctggtaaaa tgttagtttc tgcgataaat
    30601 atttcaatat cttcatctga agataaaaca aaagcatgga ataataaatc tttataacaa
    30661 gctctgcaca ataccaataa ggaaccacta tatttatcac ttaaaaattc aaaattcttg
    30721 ccaaaacccg taattctcgc ctcatttctc gtgccttggc cgtaatattt aaaattgcta
    30781 ttagtgacgc ttccatcttg ccaattaatt ttgattgaaa tagttttatt tgttcccttt
    30841 tggcaaacta catcaaagag gtcgtctgaa aaatgttttt gaatataaaa tcctgattgg
    30901 tgactaccag tagtaccaac atcattggga cgaatataac gacagtatac tgcaattgat
    30961 ttatttgctg tctgtattgc tgaagctact aagtcatcgg atttatttac tacttgtatt
    31021 gtctgagctg ccaagtcatt cattttttaa ctcatttata atttgtttag caatagcttg
    31081 aaccaacggt acagcaattg aattgccaaa ctgcttgtat gcagctgtct tggatactgc
    31141 atcaataaca aaatctttag gaaatcccat taaacgcgag cactccctag gtgtcagctt
    31201 cctaggattt tttcctttct gagggatgag tatttcggaa ccatctttgt aatatcgtgc
    31261 agatagagtt cgtgatattc catctaaatc aactaatcca aaaccaaatc cattaccctt
    31321 tgccttatgt tttttagcgt aattttgaag gtaaagccat aagttatcag aaagagtaaa
    31381 agaattatct acatcatctt ccaaaatttg ctttaatttt ggttgtgatt ctggtgggga
    31441 aggaaaattg aaatttattt ccttattaaa atattgtcta tcaaaaccta caataaaaat
    31501 acgctcccta ttttgaggaa cataatattt tgcattcata acttgataaa atatctgata
    31561 gtcaagctct tctaaagtcc ctttaattac tttaaatgta tttcctttgt catgcgaaac
    31621 aaggtttttc acattctcta aaagaaaaat tttaggtcga tgttttccaa taatttcagc
    31681 aacatcaaaa aatagagttc cctgcgcctt atctaagaag cctgtttctc gtcctaggct
    31741 ttttttcttt gaaacaccag ctatagagaa tggctgacac gggaatcctg ctgttaatac
    31801 atcaaactta cttggaatag ctgctttggt ttcctttaat gtaatatctc cataaggaat
    31861 atcattaaaa tttacttggt aggtttgacg ggctttatca tcccattcac tagaaaatac
    31921 acatcgccca ccaacattct ccattgcaat gcgaaaacca cctattcccg caaataaatc
    31981 aataaaagta aatttctcat tatttaaggt aactatatta tctaattgat tttgtatttt
    32041 atttttcata ttttatattt cagatgattt attaatctaa aggccatctg aaaactcccc
    32101 ctttcatcaa atcttacaag ccccgcccgc gcagccgtca tcctgaatat cggtctgcgt
    32161 atcgtccgca ccgtcgcggg tgttatggta gtacagggtt ttgacgccgt atttgtaggc
    32221 ggtcagcagg tctttgagca tttgtttcat agaaactttg ccgccttcga atttgcccgg
    32281 gtcgtaggcg gtattggcgg aaatcgattg atcgacgaat ttttgcatca cgccgacaag
    32341 tttcaggtag ccttcgttgc cgggaagctg ccacagggtt tcataggcat ttttcagggt
    32401 ttcaaactcc ggcacgactt gtttcaaaat gccgtctttc gatgctttga ccgttaccaa
    32461 tccgcgcggc ggctcgatgc cgttggtggc gttggcgatt tgcgaactgg tttcagacgg
    32521 catgagcgcg gtcagggtgg agttacgcag gccgtatttg acgatgtcgg cacgcaggct
    32581 ttcccagtcg taatgcaaag gctcgccgca gacggcatcc aaatcttttt tgtaagtgtc
    32641 gatgggcagt ttgccttgcg aataaacggt ttggttaaag agcgtgcacg caccgtattc
    32701 tttggcaagg tttgccgatg ctttgagcag gtaatactgt atggcttcaa aggtacggtg
    32761 ggtcagaccg agcgcggagc cgtcgctgta gcggacaccg tttttcgcca gataataagc
    32821 atagttaatc acgccgatgc cgagcgaacg gcgacccata gtagaggtac gcgcggcttc
    32881 taccggatat ccctgataat ctaaaagtgc atcgagcgca cgcacggtca agtcggcaag
    32941 cccttccaat tcgtccaagc tgtttaatgc gcccaagtta aaggcagaca gtgtacacag
    33001 ggcgatttcg ccgttcggat cgttgatatt gtccagcggt ttggtcggca gggcgatttc
    33061 catacacaag ttggactgat gaacaggcgc gacgcacgga tcgaacgggc tgtgcgtatt
    33121 gcagtgatcg acgttttgaa tgtagatgcg cccggtcccg gcacgctcct gcatcagcgt
    33181 ggaaaacagg tcggcagccg gaatgatacg cttgcgtatg ttcgggtctt gctcgtattt
    33241 cgtatagagc cgctcaaatt cgtcttggtc ggcaaaaaac gcttcgtaca atcccggaac
    33301 ctcgttgggc gaaaacagcg taatgttgcc gcccttaatc aggcgggtgt acagcaggcg
    33361 gttgatttgc acgccgtaat caagctgacg gatacggttg tcttccacgc cccggttgtt
    33421 tttcaacacc agcaggcttt cggcttcgat atgccacaag gggtagaaca aggttgccgc
    33481 gccgccgcgc acgccacctt gcgaacagga tttgaccgcc gcctgaaaca ttttgaaaaa
    33541 cgggatgcag ccggtatgcc gtgcttcgcc gccccggatt tcgctgtcca gtccgcggat
    33601 acgtccggca ttgatgccga tgcccgcacg ctgggaaacg tatttcacaa tcgcgctggt
    33661 ggtggcgttg atggaatcca ggctgtcgtc gcattcgatc aatacgcagc ttgagaactg
    33721 gcgcgtaggc gtacgcacgc cgctcataat cggggtcggc agcgatactt taaatgtgga
    33781 aacggcatcg taaaaccgtt tgacgtaatc gaggcgtgtt tctttgggat atttgctgaa
    33841 aagacacatc gccaccagaa catataagaa ctgcggtgtt tcataaatct ggcgggtaac
    33901 gcggttctgt accagatatt tgccttcgag ctgtttgaca gcggcatagg aaaaggacat
    33961 atcgcgttcg tggtcgatat aggcgttcag ttcatcaaat tcttcgcggc tgtaatcctc
    34021 aaggatatgc ctgtcgtatt ttccggcatc ggtgagcttt ttaacgtggt cgtaaaggtg
    34081 cggcggctcg tattcgccgt aggctatttt acgcagatgg aaaatcgcca aacgcgcggc
    34141 aaggtattgg tagtccggag tatcttccga aattaaatcg gcagcggcct tgatgatggt
    34201 ttcgtggatg tcgtcggtgc ggatgccgtt gtagaactgg atgtgcgatt tcaactcgac
    34261 ctgcgacacg gaaacatttt ccaatccttc cgccgcccaa gtaacgacac ggtgaatctt
    34321 atccaaatca atggcttcta atcttccgtc tcgtttggtt actttcaaat cagtcggtgt
    34381 attcatcgct tcctcttcca ctcttgatat tcaagacacg gtcttttcaa ataaattaag
    34441 acagacaata tagtggattt ttggcatttt ctccagtctt gacaacggtt gcatttttca
    34501 gtttcaggca atgcgcgata aaacccgcct gtcgtatttt tcctataata tttgttttat
    34561 ctgataattc tttaccgata aaaaacgggt aaattttttg ccttttgacc ggctccggct
    34621 acaaggcggt gaaataagga ttttccgacg aaaaaggaaa gcttccggtt ttctgcccct
    34681 gcgaattgtt aaattttgca aagtatgatt ttgccgcgcc gccgccgaca aattccattt
    34741 tcttaccgat tgaaatttat tattgagatt aatgtgttat ttgaatccgc atatcaaacg
    34801 gcaagttttg tcggctgaat gagtctgaaa ctgccgacaa tttgcccgtt ccatccttcc
    34861 atcctatact ggaaggaatg acaaactgaa aggatcgtca tatctgtcaa aaagatgctt
    34921 gccatactgt tgtctgcaat attgggactg gtatcgacaa ctgccgctgc cggtacgtca
    34981 gaacccgccc accgccatac caaacatatc cgcaaggcaa actgcacccc gaatgcagga
    35041 aatatttgga acgccgtgcc gcgtggtacc gatcgcaagg caacgtgcag gaattgcgcg
    35101 aaaacaaaaa ggcgcgcaaa gcattccgct ccctgcctta taaggaacag aaaacccaat
    35161 gccgggcggc ttatgaggct ttcgatgatt tcgacggcgg caggttccgc cgttaatccc
    35221 atataaaata
//
LOCUS       DAWFHY010000011        34988 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.011, whole genome shotgun sequence.
ACCESSION   DAWFHY010000011 DAWFHY010000000
VERSION     DAWFHY010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34988
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.011"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(152..1927)
                     /gene="ptsP"
                     /locus_tag="WGQ68_000456"
     CDS             complement(152..1927)
                     /gene="ptsP"
                     /locus_tag="WGQ68_000456"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEZ0714323.1"
                     /translation="MSIVLHGVAAGKGIAVGCAHLIARGTEEVPQYDVAEADTDAEAE
                     RFDAAVKATRKELEQLRSAIPENAPTELGAFISLHLMLLTDVTLSREPVDILREQKIN
                     AEWALKQQSDKLAAQFDNMDDAYLRERKQDMLQVVRRIHNNLIGQGNELEVADNLFDE
                     TVLIANDLSPADTVLFKEQRIAAFVTDAGGPTGHTAILGRSLDIPSVVGLHNARKLIT
                     EGETVIVDGINGVLIIAPDESVLNEYRRRAREYRSHKRDLNKLKKTAAATADGVCIEL
                     VGNIESAEDVKPLHNLGADGIGLFRSEFLYLNRDTMPSEDEQYEVYSAIVKKMKGKSV
                     TIRTVDLGVDKNPRWFGKNSTPNGSLNPALGMTGIRLCLAEPVMFRTQMRAILRAAVH
                     GPVRMMWPMITSVSEVRQCLIHLDTARRQLAERGDAFGKVGIGCMIEIPSAALTVGSI
                     LKLVDFISVGTNDLIQYILSVDRGDDSVSHLYQPGHPSVLKMLQHVIRTANRMDKDVS
                     VCGEMAGDTAFTRILLGMGLRRFSMNPNNILPVKNIILHSNIAQLENEIAKIVRCEDE
                     EKAEKLIKQMNSVSVEEEADFKGRK"
     gene            complement(1927..2196)
                     /locus_tag="WGQ68_000457"
     CDS             complement(1927..2196)
                     /locus_tag="WGQ68_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEZ0714324.1"
                     /translation="MLKQSIEIINKLGLHARASSKFTQTASQFQSEVWVTKNGSRVNG
                     KSIMGLMMLAAAKGTVIELETDGADEAEAMRALTDLINGYFGEGE"
     gene            complement(2265..2702)
                     /locus_tag="WGQ68_000458"
     CDS             complement(2265..2702)
                     /locus_tag="WGQ68_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEZ0714325.1"
                     /translation="MIGLLIITHETIGEAYRKLAHHFFPGGLPENVRILGVQPTEDQD
                     DIINNAIAALQEFPDNDGVLIMTDIFGATPCNAARRLVRENKSAILTGLNAPMMVKAV
                     QYSPAAEDLAAFTECVREAAVKGIFAITSAPEDLVCRRSGDAV"
     gene            complement(2783..3346)
                     /locus_tag="WGQ68_000459"
     CDS             complement(2783..3346)
                     /locus_tag="WGQ68_000459"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine-guanine phosphoribosyltransferase"
                     /protein_id="HEZ0714326.1"
                     /translation="MTDLETKRLETQAMLENADLLFDQGQCRAALQKVADEITRDLGG
                     KYPLLLPVMGGAVVFTGQLLPLLRFPLDFDYVHVSRYGDKLEGGAFNWKRMPDAEQIR
                     GRHVVVLDDILDEGHTMSAIQAKLLEMGAASCRAAVFANKLIDKPKPIRADYVGLDVP
                     NRYVFGYGMDAAGCWRNLGEIYALDGK"
     gene            complement(3415..4239)
                     /locus_tag="WGQ68_000460"
     CDS             complement(3415..4239)
                     /locus_tag="WGQ68_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase"
                     /protein_id="HEZ0714327.1"
                     /translation="MIKKTIGGIIPIFTAVFIPASAGAADLMLAQEYKGQDIAGWAMS
                     EKLDGVRAYWDGKHLISRQGYAFAPPKGFTAQFPPYPLDGELYSGRGQFEQISATVRS
                     VSSDWRGIRLHVFDVPKAQGNLYQRLAVATQWLKTHPNAPITIIPQIKVRDRQHAMDF
                     LKQIEAQGGEGVMLRQPESRYSGGRSSQLLKLKSQYDDECTVTRHYEGKGRNAGRLGA
                     VGCKNRYGEFRIGSGFKDKDRDNPPKIGTLITYRYRGFTQKGTPKFATFVRARTDR"
     gene            4331..4963
                     /locus_tag="WGQ68_000461"
     CDS             4331..4963
                     /locus_tag="WGQ68_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEZ0714328.1"
                     /translation="MTLRYEIFPVTPFRQNCTLIWDDESGEAVLTDVGGDVPFLLQAL
                     ANRKLTLTAIWLTHGHLDHAGGVVEMLKTHKVPVLGPHPDDEFLLQSLPQTTAQYGFP
                     VSPAFAPNRWLEEGETLTVGRYAFQVLHIPGHTPGHIVFYCAEAELLIAGDVLFYETI
                     GRTDFPRGNHADLINNIRNKLFTLPETVQVVAGHGRMTSIGHEKRHNPFF"
     gene            complement(5169..6953)
                     /gene="rmuC"
                     /locus_tag="WGQ68_000462"
     CDS             complement(5169..6953)
                     /gene="rmuC"
                     /locus_tag="WGQ68_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEZ0714329.1"
                     /translation="MELMTVLLPLAALVSGVLFTWLLMKGRFQGEFAGLNAHLAEKAA
                     RCDFVEQAHGKTVSELAVLDGKYRHLQDENYALGNRFSAAEKQIAHLQEKEAESARLK
                     QSYIELQEKAQGLAVENERLATQLGQERKAFADQYALERQIRQRIETDLEESRQTVRD
                     VQNDLADVGNRFAAAEKQIAHLQEKEAEAERLRQSHTELQEKAQGLAVENERLATQIE
                     QERLASEEKLSLLGEARKSLSDQFQNLANTILEEKSRRFTEQNREQLHQVLNPLNERI
                     HGFGELVKQTYDKESRERLTLENELKRLQGLNAQLHSEAKALTNALTGTQNKVQGNWG
                     EMILETVLENSGLQKGREYVVQAASVRKEEDGGTRRLQPDVLVNLPDNKQIVIDSKVS
                     LTAYVRYTQAADADTAARELAAHVASIRAHMKGLSLKDYTDLEGVNTLDFVFMFIPVE
                     PAYLLALQHDAGLFQECFDKRIMLVGPSTLLATLRTVANIWRNEQQNQNALAIADEGG
                     KLYDKFVGFVQTLESVGKGIDQAQSSFQTAFKQLAEGRGNLVGRAEKLRLLGVKAGKQ
                     LQRDLVERSNETTALSESLEYVAEDEAV"
     gene            complement(7292..8080)
                     /locus_tag="WGQ68_000463"
     CDS             complement(7292..8080)
                     /locus_tag="WGQ68_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEZ0714330.1"
                     /translation="MKQAMKNWFAALLLAVPMGAAFASGGHAHYEKVDIDLRDQVSLQ
                     RGAQIFTNYCLSCHSASGMRFNRLKDIGLTDEEIKKNLMFTTDNVGDVMHSAMNPKDA
                     AKWFGAAPPDLTLIARSKGADYLYAYMRGFYKDPTRPSGWNNTVFDKVGMPHPLWEQQ
                     GVQAVELDAKGQPVMVKDEHGEMKPKLYWESTGLHSRRLPNGKVIQKEYDAYVRDLVN
                     YLVYMGEPAQLQRKRIGYVVMIFLFAVMLPLAYFLKKEYWKDVH"
     gene            complement(8095..9444)
                     /locus_tag="WGQ68_000464"
     CDS             complement(8095..9444)
                     /locus_tag="WGQ68_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HEZ0714331.1"
                     /translation="MANQTNSKAKALLGWMDARFPLSKMWNEHLAQYYAPKNFNFWYY
                     FGSLALLVLVIQIVSGIFLTMNYKPDGNLNAYHLPAAFTAVEYIMRDVSGGWIIRYMH
                     STGASFFFIVVYLHMFRGLIYGSYKKPRELVWIFGSLIFLALMAEAFMGYLLPWGQMS
                     FWGAQVIINLFSAIPVIGPDLSTWIRGDFNVSDVTLNRFFALHVIAVPLVLLGLVVAH
                     IIALHEVGSNNPDGVEIKKNKDENGIPRDGIPFHPYYTVKDILGVVVFLIVFCSVLFF
                     APEGGGYFLEAPNFDAANALKTPPHIAPVWYFTPFYAILRAIPSFLGTQVWGVIGMGA
                     AVVLIALLPWLDRGEVKSVRYRGPIFKTALVLFIISFIGLGILGAMVATDTRTLVARI
                     LSFVYFAFFLGMPFYTKLDKNKPVPERVTMSTAKQKIMFFVYVGITAVGAYLFATNI"
     gene            complement(9463..10044)
                     /gene="petA"
                     /locus_tag="WGQ68_000465"
     CDS             complement(9463..10044)
                     /gene="petA"
                     /locus_tag="WGQ68_000465"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEZ0714332.1"
                     /translation="MDNQEINNGRRRFLTLATCGAGGVAALGVATPFVASFFPSEKAK
                     AAGAAVEVDVSKIEAGQLLTAEWQGKPIWVLNRTDQQLKDLKGLNGELTDPNSDAEQQ
                     PEYAKNETRSIKPNILVAIGICTHLGCSPTFRPDIAPADLGADWKGGFFCPCHGSKFD
                     LAGRVYKGVPAPTNLVVPPYKYLSDTTILVGED"
     gene            complement(10167..10916)
                     /locus_tag="WGQ68_000466"
     CDS             complement(10167..10916)
                     /locus_tag="WGQ68_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEZ0714333.1"
                     /translation="MVLRRDFLTWCNETLQTALFKDYAPNGLQVEGREYIGKIVTSVT
                     ASRAAIDFAVEQKADLLLVHHGMFWKNELPTVTGWKKERIAALLRHDINMAGYHLPLD
                     AHPTLGNNAQLADRLGFATEKRFGEQNLLNSGSLKQAKTLGALAAHIETVLQRKPVVI
                     GKPEREIRRVAWCSGGAQGFFQTAIDEGVDLYLTGEISEAQYHLANETGTAFISAGHH
                     ATERYGVRALAESAAEVFGLEVCHFDENNPA"
     gene            11047..11976
                     /locus_tag="WGQ68_000467"
     CDS             11047..11976
                     /locus_tag="WGQ68_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0714334.1"
                     /translation="MDSIIELRHLKTLLALEETGSVSLAAKRVFLTQSALSHQIRMLE
                     NHYGTPLFERKSTPLRFTPVGERLLRLAHELIPQVAVAEWDLARITEGEAGELRIAVE
                     CHTCFDWLMPAMGEFRPMWPQVELDIVSGFQADPVGLLLQHRADLAIVSEAEKQNGIS
                     FQPLFAYEMVGICAPDHPLAAKNVWTAEDFIGETLITYPVPDEMLDLPKKILIPKNIN
                     PPRRHSELTIAIIQLVASRRGIAALPYWTVMPYLEKGYVVHRQITADGLQSKLYAAIR
                     TEDTDKSYLNNFCQIIRERGFADLPGLSELESV"
     gene            complement(12231..12623)
                     /gene="rpsI"
                     /locus_tag="WGQ68_000468"
     CDS             complement(12231..12623)
                     /gene="rpsI"
                     /locus_tag="WGQ68_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEZ0714335.1"
                     /translation="MNGKYYYGTGRRKSSVARVFLIKGTGQIIVNGRPVDEFFARETS
                     RMVVRQPLVLTENAESFDIKVNVVGGGETGQSGAIRHGITRALIDFDAALKPALSQAG
                     FVTRDAREVERKKPGLRKARRAKQFSKR"
     gene            complement(12633..13064)
                     /gene="rplM"
                     /locus_tag="WGQ68_000469"
     CDS             complement(12633..13064)
                     /gene="rplM"
                     /locus_tag="WGQ68_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEZ0714336.1"
                     /translation="MKTFSAKPHEVKREWFVIDAQDKVLGRVAAEVASRLRGKHKPEY
                     TPHVDTGDYIIVINADKLRVTGAKFEDKKYFRHSGFPGGIYERTFREMQEQFPGRALE
                     QAVKGMLPKGPLGYAMIKKLKVYAGAEHAHAAQQPKVLELK"
     gene            complement(13242..13421)
                     /gene="ssrS"
                     /locus_tag="WGQ68_000470"
     ncRNA           complement(13242..13421)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="WGQ68_000470"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(13427..13732)
                     /locus_tag="WGQ68_000471"
     CDS             complement(13427..13732)
                     /locus_tag="WGQ68_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEZ0714337.1"
                     /translation="MNIEQVHIEVMHARLTVNTPAEEKDTLLQAVGMLNGKAEAIREG
                     GRVADSEKIVIMAALNVVHDLLKTSLNGGDLAIGDFARKIADMDNACQKALSRLAQE"
     gene            complement(13743..14072)
                     /locus_tag="WGQ68_000472"
     CDS             complement(13743..14072)
                     /locus_tag="WGQ68_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714338.1"
                     /translation="MKQNIEKLESSVYTLVQKFETLVSENRRLKETVAELKRAHERQK
                     LEHETAVDELSEALLVQVGKLKEDLQNKIDSLTEENTRYRSLLEQSREKISALAARLP
                     QWQETQQ"
     gene            complement(14127..15116)
                     /locus_tag="WGQ68_000473"
     CDS             complement(14127..15116)
                     /locus_tag="WGQ68_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEZ0714339.1"
                     /translation="MKITVIGAGSWGTALALHFSQHGNRVSLWTRNADQVRQMQEARE
                     NKRGLPGFSFPETLEVCADLADALKDSGLVLIVTSVAGLRSSAELLKQYGAGHLPVLA
                     ACKGFEQDTGLLTFQVLKEVLPDNKKIGVLSGPSFAQELAKQLPCAVVLASENQEWVE
                     ELVPQLNTSVMRLYGSTDVIGVAVGGAVKNVMAIATGLSDGLEYGLNARAALVTRGLA
                     EITRLASAMGAQPKTMMGLAGIGDLILTCTGALSRNRRVGLGLAEGKELHQVLVEIGH
                     VSEGVSTIEEVFNTACKYQIDMPITQTLLQLIRKEMTPQQVVERLMERSARFE"
     gene            complement(15239..17941)
                     /gene="ppc"
                     /locus_tag="WGQ68_000474"
     CDS             complement(15239..17941)
                     /gene="ppc"
                     /locus_tag="WGQ68_000474"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEZ0714340.1"
                     /translation="MQLHILNNPKDAALAADAEFLKQSLFNLLHEEASPLVVETVKLL
                     STSDDSAALIEKVLPQLDEQQTHDLTLACGLFAQILNIAEDVHHERRRQIHEEAGRGG
                     AEGSLTETVRRLKAGKADGKSVQRQLDNTSVTAVLTAHPTEVQRQTVLSFNRRIRALL
                     PQRERCTNADALARLRREIDTILLGLWQTSETRRHKLSVNDEINNGVSIFPMSFFEAL
                     PKLYRKMEHDFQTAYPDVDVPDILKIGGWIGGDRDGNPFVSAETLRFAFRRHADAVFR
                     FYRGELDKLYRELPLSIRRVKVNDDVMALAALSPDEEIARTEEPYRRAIAYIMARAMG
                     KARALGLGMGCKFGFLEPYASAQEFLDDLKKLQRSLIDNGSRLLAEGRLADLIRSVSV
                     FGFHMMPLDLRQHAGKHADVVAELFQHAGLEDYNRLNEEQKQTALLRELSHQRPLYSP
                     FITYSDHTRHELAIFNEARKIKDEFGEDAVTQSIISNCEQPSDLLALALLLKETGLLA
                     VENGKPHSRINIVPLFETIEALENACPVMETMFRLDWYDALLESRGNIQEIMLGYSDS
                     NKDGGYVTSSWCLYQAELGLVELFKKYDVRMRLFHGRGGSVGRGGGPSYQAILAQPAG
                     SVAGQIRITEQGEVITAKYADPGNAQRNLETLVAATLEASILPDKKDPDAKLMQALSD
                     VSFKYYRELITHPDFIDYFLQTSPIQEIATLNLGSRPASRKTLARIQDLRAIPWVFSW
                     MQNRLMLPAWYGFGSAVETLCEDKPETLAALREHAQSNPFFQAMLSNMEQVMAKTDIT
                     LAENYAGLSESPDKAKIIFGMIKEEYRRSRKALLDLLQTEELLRDNRSLARSLALRIP
                     YLNALNGLQVAMLKRLRKEPDNPHALLMVHLTINGVAQGLRNTG"
     gene            17984..18877
                     /locus_tag="WGQ68_000475"
     CDS             17984..18877
                     /locus_tag="WGQ68_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesA/MoeB/ThiF family protein"
                     /protein_id="HEZ0714341.1"
                     /translation="MPHPCQTVHTLTARVPKCRLKLNAARRQRYNRPQLFFPNIIMTT
                     TEHDNDDELLLRYSRHILLDEIGIEGQQKLSDAHILVVGCGGLGAAALPYLAASGVGT
                     LTIADSDTVELHNLQRQVAFDEGDVGKPKAEALAGRLKRINHTVNVRAVNEKLDGCRL
                     TGLVQAADIVLDCCDNYATRQAVNRACVQTKTPLVSGAAVRFEGQLAVYRPDLPDSPC
                     YACLFDGGSASDGICSLFGVFSPLVGIIGSTQAAEALKILLDAGEPSHGRLAVYRALE
                     GGWQYFDLPRNPECPVCGAER"
     gene            complement(19287..19511)
                     /locus_tag="WGQ68_000476"
     CDS             complement(19287..19511)
                     /locus_tag="WGQ68_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HEZ0714342.1"
                     /translation="MDAVQELERRIVELEIQSALQEDVIAGLNAMVAELRQTLDLQQA
                     QLRLLYQKMQDRNPDAQEPYSLRDEIPPHY"
     gene            complement(19512..20900)
                     /locus_tag="WGQ68_000477"
     CDS             complement(19512..20900)
                     /locus_tag="WGQ68_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HEZ0714343.1"
                     /translation="MNAVVVAVIVMLVLSLSRVHVVLSLTIGAFVGGAVAGMPLQNIA
                     DAAGQVSQAGIIPVFNKGLEGGAKIALSYAMLGAFAMAITHSGLPQQLAGAVVRKLNR
                     GGMPDSVRSGEGAVKWLLLSIILVMGMMSQNIIPIHIAFIPMIVPPLLLVFNRLKIDR
                     RLIACVITFGLVTTYMFLPYGFGAIFLNEILLGNIHSAAPQLDVKNINVMAAMAIPAL
                     GMLAGLLLAFVHYRKPRLYQSNNADTAGNADAANRPQPSAYRSLAAAAAIAVCFAIQL
                     MYEDSLVLGAMLGFAVFMMLGVINRDKANDVFGEGIKMMAMVGFIMIAAQGFAAVMNA
                     TGHIQPLVESSMAIFGNSKGMAALAMLVVGLLVTMGIGSSFSTLPIIAAIYVPLCVGL
                     GFSPLATVAIVGTAGALGDAGSPASDSTLGPTMGLNADGQHDHIRDSVIPTFIHYNIP
                     LLIAGWIAAMVL"
     gene            complement(20986..21807)
                     /gene="prmC"
                     /locus_tag="WGQ68_000478"
     CDS             complement(20986..21807)
                     /gene="prmC"
                     /locus_tag="WGQ68_000478"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0714344.1"
                     /translation="MTFDKWLGLSKLPKIEARMLLQYVSEYTRVQLLTRGGEEMPDEV
                     RQRADRLAQCRLNGEPVAYILGVREFYGRRFTVNPSVLIPRPETEHLVEAVLARLPEN
                     GRVWDLGTGSGAVAVTVALERPDAFVRASDISPPALETARENAADLGARVEFAHGSWF
                     DTDMPSEGKWDIIVSNPPYIENGDKHLLQGDLRFEPQIALTDFSDGLSCIRTLAQGAP
                     DRLAEGGFLLLEHGFDQGAAVRGVLAENGFSGVETLPDLAGLDRVTLGKYMKHLK"
     gene            complement(21797..22258)
                     /locus_tag="WGQ68_000479"
     CDS             complement(21797..22258)
                     /locus_tag="WGQ68_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714345.1"
                     /translation="MQEQNRKSSFPIVMLLVSVALWIASLSNVAFYLGNHGSMEGLTV
                     LILGSIFASLDIRYCAVYANYVWLAAIVLLALRKKVVPVHAALWGLALVAFSVKAVYV
                     DEAGNTSDIVRYGAGFYLWYAAFAVATIGTFAGKNKERKAAPAADGIKNDV"
     gene            complement(22323..23807)
                     /gene="tldD"
                     /locus_tag="WGQ68_000480"
     CDS             complement(22323..23807)
                     /gene="tldD"
                     /locus_tag="WGQ68_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HEZ0714346.1"
                     /translation="MPYNQAPDFLCADTMHPTYSAVQARLLEANRLSPELLAKSLCII
                     GAHHVDYADIYCQRTAYESWHLEEGIVKSGSFQINQGVGVRAVSGDKTAFAYADSLCI
                     DSINRSARAVRAIGAAGGKVSAKMPSETRGKPVCSASDPIAGLDSAAKVALLNKVEAI
                     AKAADPRIVQVMAGLTCEYDMVYLARLDGKHAADIRPMVRLNVTVIAKQGERREQGSA
                     GGGGRYDLAYFDENLVRQFVDAAVKQALTNLESRPAPAGEMTVVLGNGWPGVLLHEAV
                     GHGLEGDFNRKGTSVFSGRIGERVAAKGVTVVDQGDIAGRRGSLNIDDEGNETRRTVL
                     IEDGILTGYMQDETNARLTGTQSTGNGRRESYASAPMPRMTNTFMENGSYEPEEIIAS
                     IDKGIYAVNFGGGQVDITSGKFVFSASEAWWVEGGRLQYPVKGATIIGNGPEVLKHVS
                     MIGNDTALDSGVGVCGKEGQSVPVGVGQPTLRIDAGLTVGGSAI"
     regulatory      23985..24088
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            24155..25378
                     /gene="cytX"
                     /locus_tag="WGQ68_000481"
     CDS             24155..25378
                     /gene="cytX"
                     /locus_tag="WGQ68_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hydroxymethylpyrimidine transporter
                     CytX"
                     /protein_id="HEZ0714347.1"
                     /translation="MSGNASSPSSSAAIGLIWFGAAVSIAEISTGTLLAPLGWQRGLA
                     ALLLGHAVGGALFFAAAYIGALTGRSSMESVRLSFGKRGSVLFSVANMLQLAGWTAVM
                     IYAGATVSSALGKVLWDGESFVWWALANGALIVLWLVFGARKTGGLKTVSMLLMLLAV
                     LWLSAEVFSTAGSTAAQVSDGMSFGTAVELSAVMPLSWLPLAADYTRHARRPFAATLT
                     ATLAYTLTGCWMYALGLAAALFTGETDVAKILLGAGLGAAGILAVVLSTVTTTFLDAY
                     SAGASANNISARFAETPVAVGVTLIGTVLAVMLPVTEYENFLLLIGSVFAPMAAVLIA
                     DFFVLKRREEIEGFDFAGLVLWLAGFILYRFLLSSGWESSIGLTAPVMSAVAIATVSV
                     RLFFKKTQSLQRNPS"
     gene            25375..26475
                     /locus_tag="WGQ68_000482"
     CDS             25375..26475
                     /locus_tag="WGQ68_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEZ0714348.1"
                     /translation="MTRIAILGGGLSGRLTALQLAEQGYQIALFDKGCRRGEHAAAYV
                     AAAMLAPAAEAVEATPEVVRLGRQSIPLWRNIRGRLKTPAMMQENGSLIVWHGQDKPL
                     SNEFVRHLKRGGVADDEIVRWRADDIAEREPQLGGRFSDGIYLPTEGQLDGRQILSAL
                     ADALDELNVPCHWEHECVPEGLQAQYDWLIDCRGYGAKTVWNQSPEHTSTLRGIRGEV
                     ARVYTPEITLNRPVRLLHPRYPLYIAPKENHVFVIGATQIESESQAPASVRSGLELLS
                     ALYAVHPAFGEADILEIATGLRPTLNHHNPEIRYNRARRLIEINGLFRHGFMISPAVT
                     AAAVRLAVALFDGKDAPERDEESGLAYIRRQD"
     gene            26496..27113
                     /gene="thiE"
                     /locus_tag="WGQ68_000483"
     CDS             26496..27113
                     /gene="thiE"
                     /locus_tag="WGQ68_000483"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEZ0714349.1"
                     /translation="MTFLPLKSPLKFYAVVPTADWVERMVEAGADTVQLRCKALHGDE
                     LKREIARCAAACRGSRTQLFINDHWREAIEAGAYGVHLGQEDMDTADLAAIAAAGLRL
                     GLSTHSVAELDRALSVHPSYVASGAIFPTTTKQMPTAPQGLEKLREYVKQAGSTPVVA
                     IGGIDLNNARAVLATGVSSLAAVRAVTEAANPEAVVKAFQALWDG"
     gene            27535..27729
                     /gene="thiS"
                     /locus_tag="WGQ68_000484"
     CDS             27535..27729
                     /gene="thiS"
                     /locus_tag="WGQ68_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEZ0714350.1"
                     /translation="MNIILNGEPAELHGTSVADLIAQTAPQKPFAVAVNTVFVPKGAY
                     AETVLNENDKIDIVRPVVGG"
     gene            27942..28730
                     /locus_tag="WGQ68_000485"
     CDS             27942..28730
                     /locus_tag="WGQ68_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEZ0714351.1"
                     /translation="MLTLYGETFPSRLLLGTAAYPTPEILKQSVRIAQPAMITVSLRR
                     TGSGGEAHGQGFWSLLQETGVPVLPNTAGCQSVQEAVTTAQMAREVFETDWIKLELIG
                     DDDTLQPDVFQLVEAAEILIKDGFKVLPYCTEDLIACRRLLDVGCQALMPWAAPIGTG
                     LGAVHAYALNVLRERLPDTPLIIDAGLGLPSQAAQVMEWGFDGVLLNTAVSRSGDPVN
                     MARAFALAVESGRLAFEAEPVEARTKAQASTPTVGQPFWHSAEY"
     gene            28709..28997
                     /locus_tag="WGQ68_000486"
                     /pseudo
     CDS             28709..28997
                     /locus_tag="WGQ68_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696463.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
     gene            complement(29493..30317)
                     /locus_tag="WGQ68_000487"
     CDS             complement(29493..30317)
                     /locus_tag="WGQ68_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEZ0714352.1"
                     /translation="MKWLFTLLAVLNIAVFGGTVGYKLAVKATGGVPENRVVENTPPA
                     TPAADNAAASVEDTAALLKPGDILTEEQAEQLRLKKEAEQKKLREKKQREEKARREKL
                     AAEKAQAERENGAADALCAAQASLTMDEDDYHRIKGLLGKWSHVASRSVEKRTAKAKP
                     ADKTYRVVLPVSADAENQAAELSAKGFNPIPFDGALSLGVGNSRENAQALQNRLAGAG
                     FGGAHIVEHFAEADRQDDSLSVSRMTVLFTGVNAADADEIRKITSLYGKLNLKSCK"
     gene            complement(30430..32208)
                     /locus_tag="WGQ68_000488"
     CDS             complement(30430..32208)
                     /locus_tag="WGQ68_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/type III pantothenate kinase"
                     /protein_id="HEZ0714353.1"
                     /translation="MTVLKLSHWRVLAELADGLPQHVSQLARMADMKPQQLNGFWQQM
                     PAHIRGLLRQHDGYWRLVRPLAVFDAEGLRELGERSGFQTALKHECASSNDEILELAR
                     IAPDKAHKTICVTHLQSKGRGRQGRKWSHRLGECLMFSFGWVFDRPQYELGSLSPVAA
                     VACRRALSRLGLKTQIKWPNDLVVGRDKLGGILIETVRAGGKTVAVVGIGINFVLPKE
                     VENAASVQSLFQTASRRGNADAAVLLETLLAELDAVLLQYARDGFAPFVAEYQAANRD
                     HGKAVLLLRDGETVFEGTVKGVDGQGVLHLETAEGKQTVVSGEISLRSDDRPVSVPKR
                     RDSERFLLLDGGNSRLKWAWVENGTFATVGSAPYRDLSPLGAEWAEKADGNVRIVGCA
                     VCGEFKKAQVQEQLARKIEWLPSSAQALGIRNHYRHPEEHGSDRWFNALGSRRFSRNA
                     CVVVSCGTAVTVDALTDDGHYLGGTIMPGFHLMKESLAVRTANLNRHAGKRYPFPTTT
                     GNAVASGMMDAVCGSVMMMHGRLKEKTGAGKPVDVIITGGGAAKVAEALPPAFLAENT
                     VRVADNLVIHGLLNLIAAEGGESEHT"
     gene            complement(32205..32711)
                     /locus_tag="WGQ68_000489"
     CDS             complement(32205..32711)
                     /locus_tag="WGQ68_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HEZ0714354.1"
                     /translation="MVDAWSVPDFESKICPPEALAARLALLPRPLVFTNGCFDILHRG
                     HVTYLAQARSMGGALVLALNTDASVRRLGKGGDRPINPLENRAAVAAALESVDLVTWF
                     DGDTPAALIEAVKPEILVKGGDWAADKIVGAAETLARGGQVFSIPFLHQTSTTKTLAK
                     IRAAEGGK"
     gene            complement(32847..32922)
                     /locus_tag="WGQ68_000490"
     tRNA            complement(32847..32922)
                     /locus_tag="WGQ68_000490"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32887..32889),aa:His,seq:gtg)
     gene            complement(32950..33026)
                     /locus_tag="WGQ68_000491"
     tRNA            complement(32950..33026)
                     /locus_tag="WGQ68_000491"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32990..32992),aa:Arg,seq:tct)
     gene            complement(33051..33128)
                     /locus_tag="WGQ68_000492"
     tRNA            complement(33051..33128)
                     /locus_tag="WGQ68_000492"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33091..33093),aa:Pro,seq:tgg)
     gene            33351..34205
                     /gene="folD"
                     /locus_tag="WGQ68_000493"
     CDS             33351..34205
                     /gene="folD"
                     /locus_tag="WGQ68_000493"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEZ0714355.1"
                     /translation="MSAQLINGKEVSQKRLQAVAEAVAQRQQNNLHTPCLAVVLVGGD
                     PASAVYVRNKKTSCQKCGIKSLSYELPESTSQEELLALVDRLNVDSEVDGILVQLPLP
                     KHLDSQAVLERISPDKDVDGFHPYNVGRLAVKMPLMRPCTPKGVMTLLEAYGIDPKGK
                     KAVVVGASNIVGRPQALELLLARATVTVCHSATENLADEVAGADILVVGVGIPNFVKG
                     EWIKPGAVVIDVGINRLDDGSLCGDVEFETAKERAAMITPVPGGVGPMTIATLMENTL
                     HAASLHDA"
     gene            complement(34267..34848)
                     /locus_tag="WGQ68_000494"
     CDS             complement(34267..34848)
                     /locus_tag="WGQ68_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0714356.1"
                     /translation="MEEKNDYTDAASDNRNGQEIEVGIAGAGDRILAALLNQLFTFLV
                     LLVPFAGLIAFAIKNEGRIGGSEEIFGLLLGMTSFWVGLAGILVYTVVQIYYMSRDGQ
                     SLGKKIMRIRVLKTDGRNPGFVGTVLVREIAWSVLVAIIAAVIGLAVGDNGENAINLL
                     AFLANFVLLFMVKRDRRTLYDILADTVVVKLPK"
BASE COUNT         7650 a        10019 c         9245 g         8074 t
ORIGIN      
        1 gggaatgacg gattttaggt ttctgatttc ggttttctgt tttaagggaa tgaccgattt
       61 tgggtttctg tttcggtttt ctattttgca agaatggcaa aatttcagat tgcgggcatt
      121 gttaagtatt tctatttttt acctgccgta tttatttccg ccccttgaag tcggcttctt
      181 cctcgacaga cacgctgttc atctgtttga tcagcttttc cgccttttcc tcgtcctcgc
      241 agcggacgat tttggcaatc tcgttttcga gctgtgcgat attgctgtgc aggatgatgt
      301 ttttgacggg caggatgttg ttcggattca tggaaaaacg gcgcagcccc ataccaagca
      361 ggatgcgggt aaacgccgta tcgcccgcca tctcgccgca tacggatacg tctttgtcca
      421 tgcggttggc ggtacggatg acgtgttgca gcattttcaa cacggaagga tgtccgggct
      481 ggtagagatg gctgacgctg tcgtcgccgc gatcgacgga caagatgtat tgaatcaggt
      541 cgttggtacc gacggagatg aaatcgacca gtttcaaaat actgccgacg gtcagcgcgg
      601 cagacggaat ttcaatcata cagccgatgc cgactttacc gaaggcatcg ccgcgttcgg
      661 caagctggcg gcgggcggtg tccaaatgaa tcaggcactg gcgcacttcg gatacggagg
      721 taatcatcgg ccacatcatc cgcacagggc cgtgtaccgc cgcacggagg atggcgcgca
      781 tctgggtgcg gaacatgacc ggttcggcaa ggcacaggcg gatgccggtc atgcccagcg
      841 cggggttgag gctgccgttg ggcgtgctgt ttttcccgaa ccagcggggg tttttatcca
      901 cgcccaaatc gactgtccgt atcgttacgc ttttgccctt catttttttg acaatcgcgc
      961 tgtacacttc gtactgctcg tcttcagacg gcatcgtatc gcggttcagg taaagaaact
     1021 cgctgcggaa cagcccgatg ccgtctgcgc cgaggttgtg cagcggtttc acgtcttcgg
     1081 cggattctat attgcccaca agctcgatgc agaccccgtc agcggtggcg gcggcggttt
     1141 ttttgagctt gttcaaatcg cgtttgtggc tgcggtattc gcgggcacgg cggcggtatt
     1201 cgttcaacac cgactcatcc ggcgcgataa tcaacacgcc gttgataccg tccacaatga
     1261 ccgtttcgcc ctcggtaatc agtttgcgcg cgttgtgcag cccgacgacg gacgggatgt
     1321 ccaagctcct gcccaaaatc gccgtatgcc cggtggggcc gccggcatcg gtaacgaagg
     1381 cggcaatgcg ctgctcttta aacaaaaccg tgtcggcggg cgaaaggtcg tttgcaatca
     1441 gaacggtttc gtcaaacagg ttgtcggcaa cttccaactc gttgccctgc ccgatcaggt
     1501 tgttgtggat gcggcggacg acttgcagca tatcctgctt gcgttcgcgc aaataggcat
     1561 cgtccatatt gtcgaattgg gcggcgagtt tgtcgctctg ctgcttcaat gcccactcgg
     1621 cgttgatttt ttgttccctt aaaatatcga cgggttcgcg cgacaaggta acatcggtca
     1681 agagcatcag gtgcagcgag atgaacgcgc ccaactcggt cggggcgttt tcgggaatcg
     1741 cgctgcggag ctgttccaac tctttgcgcg tggctttgac ggcggcatcg aaacgttcgg
     1801 cttcggcatc ggtgtccgcc tccgcaacat catactgcgg cacttcctcc gtaccgcgcg
     1861 caatcaggtg ggcgcaaccg acggcaatgc ctttgcccgc cgccacgccg tgcagcacga
     1921 tactcattat tcgccctcgc cgaagtagcc gttgattaag tcggtcaggg cgcgcatcgc
     1981 ttccgcctcg tccgcgccgt ccgtctccag ctcgatgacc gtacccttgg cggcggcgag
     2041 catcatcagc cccatgatgc ttttgccgtt gacgcggctg ccgtttttcg ttacccagac
     2101 ttcgctttga aactgagacg cggtttgggt gaatttgctg gaggcacggg cgtgaagtcc
     2161 gagtttgttg atgatttcga tggattgttt gagcatttcg attcccgtgt tatgtatatc
     2221 ggcagcagac gccgtttaaa atgttttcct gccctgccgc ttcttcagac ggcatcgccg
     2281 ctgcgccggc acaccaaatc ttcgggcgcg gacgtgatgg cgaaaatgcc ttttacagcc
     2341 gcctctctga cacattcggt aaaggcggca aggtcttccg ccgccggcga atattggacg
     2401 gccttaacca tcatcggcgc gttcagtccg gtcaaaatcg ccgatttgtt ttcgcgcacg
     2461 aggcggcggg ctgcattgca gggggtcgcg ccgaaaatat cggtcataat cagcacgccg
     2521 tcgttgtcgg gaaattcctg aagcgcggca atggcgttgt tgatgatgtc gtcttggtct
     2581 tccgtcggtt gcacgccgag tatgcggacg ttttcaggca gcccgcccgg gaaaaaatga
     2641 tgtgccagct tgcggtaggc ttcgcctatg gtttcgtgtg tgatgattaa aagccctatc
     2701 atattatgcg tcctgttcct cgttgtccta ccggcgtatg ggcgcgatgc cgtctgaaag
     2761 gccttcagac ggcatcgcgt ccttattttc cgtccaatgc gtaaatctcg cccagattgc
     2821 gccagcagcc cgccgcatcc atgccgtaac cgaaaacata acggttcggc acatccagtc
     2881 cgacataatc ggctcggata ggcttgggtt tgtcaatcag cttgttggcg aacaccgccg
     2941 cacggcagct tgccgcaccc atttccaaaa gtttggcttg aatggcggac atcgtatgcc
     3001 cttcgtccaa aatatcgtcc agcacgacga cgtgcctgcc ccggatttgt tccgcatcgg
     3061 gcatacgctt ccagttgaac gcgccgccct ccagcttgtc gccgtaacgg gaaacgtgaa
     3121 cataatcaaa atctaaggga aaacgcaaca gcggcagcaa ctgccccgta aacaccaccg
     3181 cgccgcccat cacgggcagc agcagcggat atttgccgcc caaatcacgc gtaatctcgt
     3241 ccgccacttt ttgcagtgcg gcacggcatt ggccttggtc gaacaaaaga tcggcgtttt
     3301 caagcatcgc ctgtgtttca aggcgtttgg tttctaaatc ggtcatatgt cggaatcggt
     3361 cggtaaaagg aaaattataa accaaagtat cggctgccgt ctgaaccgtc ctgctcaacg
     3421 gtcggtacgc gcgcgcacaa acgtggcgaa tttcggcgtg cctttctgcg taaagccacg
     3481 gtagcggtag gtaatcagtg tgccgatttt gggcgggttg tctcggtctt tatctttgaa
     3541 gccgctgcca atgcggaatt cgccgtaccg gtttttgcag ccgaccgcgc ccaaccgtcc
     3601 ggcgtttcgc cctttgccct catagtgccg cgttaccgtg cattcgtcgt cgtattggct
     3661 tttcagcttc aataattggc tgctcctgcc gccgctgtaa cgggattcgg gctgacgcag
     3721 catcacgcct tcgccgccct gcgcttcgat ttgttttaaa aagtccatcg cgtgctgccg
     3781 gtcgcgcact ttgatttgcg ggatgatggt aatcggcgcg ttcggatgcg ttttcagcca
     3841 ctgcgttgcg actgccaaac gttggtagag attgccctgc gccttgggta catcgaaaac
     3901 gtgcaggcgg atgccgcgcc agtctgaaga aacagaacgc acggtagcgg aaatctgctc
     3961 gaactgacca cgtccgctat acaattcgcc gtccaaagga taaggcggaa actgagcggt
     4021 aaaacctttg ggcggagcaa acgcataccc ctgacggctt atcaggtgct ttccgtccca
     4081 ataggcgcgc acgccgtcga gtttctcgct catcgcccag ccggcaatgt cctgcccttt
     4141 gtattcctgc gccagcatca aatccgccgc gcctgccgat gcagggatga aaaccgccgt
     4201 aaaaatcggt atgatgccgc cgattgtctt cttaatcatc tgattccccc aatatcaaaa
     4261 cgggcggcaa accgccataa aacaaacggc aaacccgatg ccgtctgaaa aaccgtttag
     4321 gaacacgccg atgaccctac gttacgaaat cttccccgtt acccccttcc gccaaaactg
     4381 caccctgatt tgggacgacg aaagcggcga agccgtcctg accgatgtcg gcggcgacgt
     4441 gccgttcctg ctgcaagcgt tggcaaaccg caaacttacg ctcacggcaa tctggctgac
     4501 gcacggccat ctcgatcacg cgggcggcgt ggtcgaaatg ttgaaaacgc ataaagtccc
     4561 tgtcctcggg ccgcatccgg acgatgaatt cctgctccaa tcgctgccgc aaaccaccgc
     4621 gcaatacgga tttcccgtct cgcccgcctt tgcgccgaac cgttggctcg aagaaggcga
     4681 aacgctcacg gtcggacgct atgcctttca agtgctgcat attccgggcc atacgccggg
     4741 acatatcgtc ttttattgtg ccgaggcgga attgctgatt gcgggcgacg tgctgtttta
     4801 cgaaaccata ggcagaaccg attttccgcg cggcaaccac gccgacttaa tcaataatat
     4861 ccgcaacaaa ttattcaccc ttcccgaaac cgtgcaagtt gtcgccggac acgggcgtat
     4921 gacttccatc ggacacgaaa agcggcacaa tccgtttttc taaccgcctt ccctacggtc
     4981 ttcagacggc atcatctgca ctgatgccgt ctgaaacgca aaaggctcag acaaccgccg
     5041 ccttgccggt gtacctcgcc ggacaaggct ttggtaagta cttcaaacca accccaaatc
     5101 agaaccggta cagttacgcc gcgctttcgg cattcccgcc cctgctgaaa caagcttttc
     5161 cgcacaagtc agactgcttc atcttctgcc acatattcca aagattccga caacgccgtt
     5221 gtttcattgg aacgctcgac caaatcccgt tgaagttgtt tgcctgcctt cacgcccaac
     5281 agacgcagtt tctcggcgcg tccgaccaga ttcccgcgcc cttcggcaag ttgcttgaat
     5341 gccgtctgaa aactgctttg cgcctgatcg atgcctttgc cgacgctttc gagcgtctgt
     5401 acgaagccga caaacttgtc gtacagcttg ccgccttcgt ccgcaatcgc cagtgcgttc
     5461 tgattttgct gttcgttgcg ccaaatattc gccaccgtcc tcaaagtcgc cagcagcgta
     5521 ctggggccga ccagcataat ccgtttgtcg aaacactctt ggaacaatcc cgcgtcatgc
     5581 tgcaacgcca gcaggtaggc cggttcgaca gggataaaca taaagacgaa atccaatgtg
     5641 ttcacacctt ccaaatcggt gtaatccttc agcgacaagc ctttcatgtg tgcacggatg
     5701 ctggcaacgt gtgccgccag ttcgcgtgcc gccgtatccg catccgccgc ctgcgtgtag
     5761 cgcacataag ctgtcagcga gaccttggag tcaatcacaa tctgcttgtt gtcgggcagg
     5821 ttgaccaaaa cgtcgggctg gaggcggcgc gtgccgccgt cttcctcttt tcggacggat
     5881 gccgcctgaa ccacatattc gcgccctttc tggaggccgg aattttccaa aaccgtttcc
     5941 agaatcattt cgccccaatt gccctgaacc ttattctgcg taccggtcag cgcgttggtc
     6001 agtgcctttg cctcgctgtg cagctgcgca ttcaatcctt gaagccgttt caattcgttt
     6061 tccaacgtca gccgctcgcg cgattcttta tcataggttt gcttgaccaa ctcgccgaaa
     6121 ccgtggatgc gttcgtttag cgggttcaaa acctgatgga gctgctcgcg gttctgctcg
     6181 gtaaaacggc ggcttttttc ttccaaaatc gtgttggcaa gattttgaaa ctgatcgctc
     6241 aaacttttgc gcgcctcgcc cagtaaggac agcttctctt cagaagcaag gcgttcctgt
     6301 tcgatttgcg ttgccaaacg ttcgttttca accgccaaac cctgtgcctt ttcctgcaac
     6361 tcggtatgcg actgcctcaa ccgctccgct tccgcctctt tttcctgcaa atgggcaatc
     6421 tgtttttcgg ctgcggcaaa acggttgccc acatcggcaa ggtcgttttg cacgtcgcgg
     6481 acagtttggc ggctttcttc caaatcggtt tcgattcttt ggcggatttg gcgttccaag
     6541 gcatattggt cggcaaacgc cttccgttcc tgtccgagct gcgttgccaa acgttcgttt
     6601 tcaaccgcca aaccctgtgc cttttcctgc aactcgatat acgactgctt cagccgcgcc
     6661 gactccgcct ctttttcctg caaatgggca atctgctttt cggctgcgga aaaacggttg
     6721 cccaaagcat aattttcgtc ctgcaaatgc cggtatttcc cgtccaacac cgccaattcc
     6781 gacacggttt tgccgtgtgc ctgttcgaca aaatcacatc ttgccgcctt ttccgccagg
     6841 tgcgcgttca aaccggcaaa ctcgccctga aacctgccct tcatcagcaa ccatgtaaac
     6901 aacacgcccg acaccaacgc cgccaaaggc agcaaaacag tcatcagttc catcaatcat
     6961 cctaatattc aaacattttc acaccggaca aaaccgccgc ttattccgat tctacctgtt
     7021 tgttcgacat agctccaaaa aatatagcgg attggcttta aacctgttcg acatcgcctt
     7081 accatgctgc ttgcggtttc agaccttttc ctaattcaat atcaatctgc cacaaaccct
     7141 gattaagttc ccgatgtctg acatttttag aatgatgccg tctgaaatgt tgcagctatg
     7201 ttcagacggc atacggattc aggcttttca aacggcaggc aaaatgaaaa aagggcaaac
     7261 cctaaaggat ttgccctttt gttccaaacg cttagtgtac gtctttccaa tattcttttt
     7321 tcaggaaata cgccaaaggc agcataaccg caaataggaa aatcatcacg acatagccta
     7381 tacgtttgcg ttgcagttgt gcaggttcgc ccatgtacac aaggtaattg accaaatcgc
     7441 gtacatatgc gtcgtactct ttttggatca ctttgccgtt aggcagacgg cggctgtgca
     7501 aaccggtaga ttcccaatac agcttaggct tcatctcgcc gtgttcgtct tttaccataa
     7561 ccggctgacc tttggcatcc aactcaacgg cttgaacgcc ttgctgttcc cacaacgggt
     7621 ggggcatacc gactttatcg aatacagtat tgttccagcc gctcggacgg gtcggatctt
     7681 tatagaagcc gcgcatatag gcgtaaaggt agtctgcacc tttggaacgc gcaatcaacg
     7741 tcaaatcggg cggagcagca ccaaaccatt ttgccgcatc tttcgggttc atcgccgaat
     7801 gcatgacatc gccgacatta tcggtggtaa acatcaggtt tttcttgatt tcttcgtcag
     7861 tcaaaccgat gtctttcaaa cggttgaaac gcataccgct tgccgagtgg caggacaggc
     7921 aatagttggt aaagatttgt gcgccgcgct gcaggctgac ttggtcgcgc aaatcgatat
     7981 cgactttctc atagtgtgca tgaccgcccg aagcaaatgc cgcacccata ggtactgcca
     8041 acagcaaggc agcaaaccaa tttttcatag cttgtttcat tttgactgcc ctcatcagat
     8101 gttggttgca aacaggtaag cacccacggc ggtaatgccg acgtaaacaa agaacatgat
     8161 tttttgtttg gcggtgctca tggttacgcg ttcgggaacc ggtttgtttt tatccagctt
     8221 ggtatagaac ggcataccca ggaagaatgc gaaatagacg aaggacagga tgcgcgcgac
     8281 caaagtacgc gtatcggttg ccaccatcgc gcccaaaata cccaaaccga taaaggaaat
     8341 gatgaacaag accaatgcgg ttttgaaaat cgggccgcgg taacggacgg atttcacctc
     8401 acccctatcc agccaaggca gcagggcaat caacacgacc gccgcgccca taccgattac
     8461 accccaaacc tgcgttccca agaacgaggg aatcgcacgc aaaatggcgt agaacggtgt
     8521 gaaataccat accggcgcaa tgtgcggagg tgttttcagc gcatttgccg catcgaagtt
     8581 cggcgcttcg aggaagtagc cgccaccttc tggtgcaaag aacaacacgg aacagaagac
     8641 aatcaggaat acaacgacac ccaagatgtc cttaacggtg tagtaaggat ggaatgggat
     8701 accgtcgcgc gggataccgt tttcgtcttt gtttttcttg atttcaacgc cgtcagggtt
     8761 gttagaaccc acttcgtgca gggcgatgat gtgcgccaca accaagccga gcaataccag
     8821 aggtaccgcg ataacgtgca gggcaaagaa gcgattcagg gtaacgtcgg aaacgttgaa
     8881 gtcgccgcga atccaagtgg acaaatccgg accgataaca gggatggcgg agaacaggtt
     8941 aataattacc tgcgcacccc aaaaggacat ctgaccccaa ggcagcaggt agcccataaa
     9001 ggcttccgcc atcaatgcca agaaaatcag agaaccgaaa atccacacca actcacgggg
     9061 ttttttgtac gaaccgtaaa tcaaaccacg gaacatgtgc agataaacaa caatgaagaa
     9121 gaaagatgcg ccggtggaat gcatgtagcg gataatccaa ccgccggaca cgtcgcgcat
     9181 gatgtactcg actgcagtaa aggcagcagg cagatggtag gcattcaggt taccgtccgg
     9241 tttgtagttc atcgtcagga aaataccgct gacgatttga atcacgagga caagcaaggc
     9301 taaagagccg aagtagtacc aaaaattaaa gtttttcggt gcgtagtatt gagccaaatg
     9361 ctcattccac attttagaca gggggaaacg agcgtccatc cagcctaaca atgcttttgc
     9421 tttgctattg gtttggtttg ccataattat cgttccttat tcttagtctt cgcccaccaa
     9481 gatagttgtg tcgctcaagt atttatatgg cgggacaacc aggttggtcg gggcaggaac
     9541 acctttatat acgcggccgg ccaagtcgaa tttcgaaccg tggcaggggc agaagaagcc
     9601 gcctttccag tctgcaccca aatcggcggg ggcaatgtcg ggacggaagg tgggcgagca
     9661 gcccaaatgg gtgcagatac cgatggcgac aaggatgttc ggcttaatcg aacgggtctc
     9721 gtttttagca tactccggct gctgttccgc atcggaattg ggatcggtaa gttcgccgtt
     9781 caggcctttc aggtctttaa gctgctgatc tgtacggttg agcacccaaa tcggtttgcc
     9841 ttgccactcg gcggtcagca gctgacccgc ttcgatttta ctgacatcca cctcgacggc
     9901 agcaccggcg gccttggctt tttccgaagg gaaaaaactg gccacaaacg gcgttgccac
     9961 acccaatgct gccactccgc ccgcgccgca ggtcgcgagt gtcaggaaac ggcggcggcc
    10021 gttgttgatt tcttgattat ccattattca gtcgtcctaa tattttggga ataccgagcc
    10081 attaaacgtt gcaattttac ccagtttgca gtgatactca aagcattatt taaaataagg
    10141 taaagtttta tgatatttct caagactcaa gccggattgt tttcgtcaaa atggcacact
    10201 tccaacccga aaacctctgc cgccgattct gccagcgcgc gtacgccgta acgttccgtc
    10261 gcgtgatgcc ctgccgaaat gaaagccgta cccgtttcat tggcaaggtg gtattgagct
    10321 tcagagattt cccccgtcaa atacagatcg acaccttcgt ctattgccgt ctgaaaaaac
    10381 ccctgcgccc cgccgctgca ccatgcaacc cgtcggattt cgcgttcggg tttgccgatg
    10441 acgacaggct tacgttgcaa aactgtttca atatgcgccg ccaatgcgcc gagtgtcttg
    10501 gcttgtttca ggctgcccga gttgagcagg ttttgttcgc cgaaccgttt ttctgtcgca
    10561 aaacccaatc tgtcggcgag ttgggcattg ttgcccagtg tgggatgtgc atccaggggc
    10621 agatggtagc ctgccatatt gatgtcgtgc cgtaacagtg cggcaatccg ttcttttttc
    10681 caaccagtaa cggtcggcaa ctcgtttttc caaaacatac cgtgatgtac caaaagcaaa
    10741 tctgccttct gctccacagc aaaatcaatc gctgccctgc ttgccgttac tgacgtaacg
    10801 attttcccga tatattccct cccttcaacc tgcaaaccgt taggggcgta atctttaaac
    10861 aacgctgtct gcaatgtttc attacaccaa gtcagaaaat ccctgcgcaa taccatcttt
    10921 tttcctaatc gctttaaaca agcgggcatt ctaatcgcaa aatgtccgga attcacattt
    10981 ttccgatttg cacccgcata tgaattattt taatatgcgc cggttcaata tgccgtctga
    11041 agccccatgg attccattat cgaattgcgc cacctcaaaa ccctgctggc acttgaagaa
    11101 accggcagcg tctcccttgc cgccaaacgg gttttcctta cccaatccgc cctttcccac
    11161 cagatccgta tgctcgaaaa ccactacggc acgccgctgt tcgaacgcaa atccacgccc
    11221 ttgcgcttta ccccggtggg cgaaaggctg ctgcgcctcg cccacgaact gatacctcaa
    11281 gttgctgttg cagaatggga tttagcgcga atcacggaag gagaggcggg agagctgcgg
    11341 attgccgtcg aatgccatac ctgtttcgac tggctgatgc ccgccatggg cgaattccgc
    11401 ccgatgtggc cccaagtcga attggatatc gtatcgggat tccaagcgga tcccgtcgga
    11461 ctgctgctgc aacaccgtgc cgaccttgcc attgtttccg aagcggaaaa acaaaacggt
    11521 atttctttcc aaccgctgtt tgcctacgaa atggtcggca tttgcgcacc agaccatccg
    11581 cttgccgcca aaaacgtttg gacggcggaa gactttatcg gggaaaccct gattacttat
    11641 cccgttcccg acgagatgct ggatttgccc aaaaaaatcc tgattccgaa aaacatcaac
    11701 ccgccgcgcc gacacagcga actgaccatc gccattatcc aactggttgc cagcagacgt
    11761 ggcattgccg cccttcctta ttggacagtc atgccctacc ttgaaaaagg ctatgtcgtc
    11821 caccgccaaa ttactgccga cggactgcaa agcaaactgt atgccgccat ccgtaccgaa
    11881 gatacggaca agagctatct gaacaatttt tgccaaatca tacgcgaacg cggttttgca
    11941 gatttgcccg gactgagcga actggaatcg gtctgacccc ttattcaacc atacccggca
    12001 gtttttctat tttttcatgt atagtggatt aacaaaaacc agtacagcgt tgcctcgcct
    12061 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac
    12121 tatactgttt ttgatttttg cccaatctgt aatctttaga ttgccaatgg gaaaccgtct
    12181 actacaaata aaaaaccctg cgataagcag ggttttttga atttccaaca ttaacgtttg
    12241 gagaattgtt ttgcacggcg tgctttgcgc agacccggtt ttttacgttc aacttcgcgg
    12301 gcatcgcggg taacaaaacc agcttgagac aaggcgggtt tcaacgcggc atcgaagtcg
    12361 atcagggcac gggtaatgcc gtggcggatt gcgccggact ggccggtttc gccgccgcca
    12421 acaacattga ctttgatgtc gaaagattcg gcgttttcag tcagaaccaa gggttggcga
    12481 acaaccattc ggctggtttc gcgtgcgaag aattcgtcaa cgggacgacc gtttacgatg
    12541 atttgacctg tacctttaat caggaataca cgagccactg aacttttgcg gcggcctgtg
    12601 ccgtagtagt atttaccgtt catgtcgcgt ccttatttca gttccaaaac tttgggttgt
    12661 tgcgcagcat gggcgtgttc cgcacccgca tacactttca gttttttaat catggcgtaa
    12721 cccagaggac ctttgggtag catacctttt acagcttgtt ccaaagcgcg gcccgggaat
    12781 tgctcttgca tttcgcggaa agtgcgttcg tagataccgc ctgggaaacc ggaatggcgg
    12841 aagtattttt tatcttcgaa tttggcaccg gttacacgca gtttgtccgc attgataaca
    12901 atgatgtaat cgccggtatc gacgtggggg gtgtattcag gtttgtgttt gccacgcaga
    12961 cggctggcga cttcggccgc aacgcgaccc aagactttgt cttgggcatc gatgacgaac
    13021 cattcgcgct tcacctcgtg gggtttcgct gaaaaggttt tcatagtgga aatccagata
    13081 gatatagaaa gttgtaaatt ttaaagacag gattcgattt tgtcaatcgc attaccgcgt
    13141 tacggaagga ttttccggat ttcggcagac tgcatactgc tttttcgggc ggggcggcgg
    13201 ccaatgtgaa aaaccgcatc gttgcgatgc ggttttgaat gggaatcccc gcgagagccg
    13261 tttcggccga atccgcttga accttgctga caaggcggct gcctcgggta gtttcgggtg
    13321 cgtccgcaaa aggacgctcg cgcccactac tgctcccggc aaccttaagc gaacttattg
    13381 gttcaaagga atatatgcct tcgcggacac cgcagggaaa aaggagttac tcctgcgcca
    13441 agcgggatag tgctttttgg caggcgttgt ccatatcggc tattttacgc gcaaaatcgc
    13501 cgattgccaa atcgccgccg ttcagggagg ttttcaacag gtcgtggacg acgttgagcg
    13561 cggccataat gacgattttt tcgctgtccg cgacgcgtcc gccttcgcgg atggcttcgg
    13621 ctttgccgtt gagcattccg actgcctgca acagtgtgtc tttttcttct gccggcgtgt
    13681 tgacggtcag ccgggcgtgc atgacttcga tgtggacttg ttcgatgttc atcctttaat
    13741 ccttattgct gcgtttcctg ccattggggg aggcgcgctg ccagtgcgct gattttttcc
    13801 ctgctctgtt cgagcaggct gcggtatcgt gtattttctt ctgtcaggct gtcgattttg
    13861 ttttgcaggt cttctttgag tttgccgact tggacgagca gggcttcgct gagttcgtcg
    13921 acggcggttt cgtgttcgag tttttgccgc tcgtgcgccc gtttgagttc ggcgacggtt
    13981 tctttgaggc ggcggttttc gctgacgagg gtttcgaatt tttgtaccag cgtataaacg
    14041 ctgctttcga gtttttcgat attttgtttc ataaccttac ctgtccgtat gccgtctgaa
    14101 ggcctcagac ggcatctgtc tgttgtttat tcgaaacgcg cgctgcgttc catcagtctt
    14161 tcgacgacct gctgaggggt catttctttg cggatgagtt gcagcagggt ttgggtaatc
    14221 ggcatgtcga tttggtactt acaggcagta ttgaagactt cttctatcgt gctgacccct
    14281 tcggaaacgt gtccgatttc gaccagcacc tgatgcagtt ccttgccttc tgccaaaccc
    14341 aagccgacgc ggcggttgcg cgaaagtgcg ccggtgcagg taaggatgag gtcgccgata
    14401 ccggcaagcc ccatcatggt tttgggctgt gcgcccattg cggaggcaag gcgggtgatt
    14461 tcagctaatc cgcgcgtaac cagtgcggca cgggcgttaa gcccgtactc taggccgtcg
    14521 gacaatccgg tggcaatcgc cataacattt tttaccgcgc cgccaaccgc cacgccgatg
    14581 acatccgtgc tgccgtaaag ccgcatgacc gaagtgttga gttgtgggac aagttcctcg
    14641 acccattctt ggttttcgga agcgaggacg acggcacagg gcagttgttt ggcgagttcc
    14701 tgtgcaaaac tcgggccgga gagtacgccg atttttttat tgtcgggcaa tacttctttc
    14761 aatacttgaa aggtcagcag ccctgtatcc tgctcgaatc ctttgcaggc ggcgaggacg
    14821 gggaggtgtc ccgcgccgta ctgtttgagc agctctgcgc tgcttctcaa tccggcaacg
    14881 gaggttacga taaggacaag tccgctgtct ttgagcgcgt ctgccaaatc cgcacacact
    14941 tccaaggttt cgggaaagga aaagccgggc agtccgcgtt tgttttcacg cgcttcctgc
    15001 atttggcgga cttggtctgc gttgcgcgtc cacagggata cgcggttgcc gtgttgggaa
    15061 aagtgcaggg cgagcgccgt accccacgaa cctgcgccga taacggtaat tttcattggt
    15121 cgtctttcaa catatcactg ccgttcactt taaaacaatc ggtgtttctc tgcaagtgcg
    15181 gtcagggaaa tgccgtctga aaggtattca gacggcattt tgtcccgatg tggcactatc
    15241 agccggtatt acgcaaacct tgcgccacgc cgttgatggt caggtgaacc atcagcagcg
    15301 cgtgcggatt gtcgggttct ttacgcaggc gtttgagcat agcgacttgc aaaccgttga
    15361 gcgcgttcag gtagggaatc ctcaaagcga gcgaacgggc gaggctgcgg ttgtcgcgca
    15421 aaagctcttc ggtttgcagt aggtcgagca gtgctttgcg gctgcggcgg tattcttcct
    15481 taatcatccc gaagatgatt tttgccttat cgggcgattc gctcaagccg gcatagtttt
    15541 ccgcgagggt gatgtcggtt ttcgccatca cttgttccat attggagagc atggcttgga
    15601 agaacgggtt gctttgggcg tgttcgcgca gggcggcgag cgtttcgggt ttgtcttcgc
    15661 acaaggtttc caccgcgctg ccgaaaccgt accaagccgg cagcatgagg cggttctgca
    15721 tccaggaaaa tacccacgga atcgcgcgca agtcctgaat ccgcgccaag gttttgcggc
    15781 tggcgggacg gctgcctagg ttgagggtgg cgatttcctg aatcgggctg gtttgcagaa
    15841 agtagtcgat gaagtcggga tgggtaatca gttcgcggta gtatttgaac gatacgtccg
    15901 acaatgcctg catcagtttg gcatcagggt cttttttatc cggcaggatg ctggcttcca
    15961 aagtcgcggc aaccaaggtt tccaagttgc gttgggcatt gccggggtcg gcgtatttgg
    16021 cggtaatgac ttcgccttgt tcggtgatgc ggatttgtcc cgccacgctg cccgccggtt
    16081 gggcgagaat ggcttggtaa gaagggccgc cgccgcgacc tacgctgccg ccgcgtccgt
    16141 ggaacaggcg catacggaca tcgtattttt tgaagagttc gaccaagccc aattccgcct
    16201 gatacagaca ccatgagctg gtaacgtagc cgccgtcctt gttggagtcg gaatagccga
    16261 gcatgatttc ttggatgttt ccacggcttt cgagcagtgc atcgtaccag tcgaggcgga
    16321 acatggtttc catgaccgga caggcgtttt ccaacgcttc aatggtttca aacagcggca
    16381 cgatattgat gcggctgtgc ggtttgccgt tttccaccgc caacaggccg gtttctttca
    16441 gcagcaatgc caaggcgagc aggtcgctgg gttgttcgca gttggaaata atgctttgtg
    16501 ttacggcatc ttcgccaaat tcgtctttga ttttgcgcgc ttcgttgaaa attgccagtt
    16561 cgtggcgggt atggtcgctg tatgtgataa acgggctgta cagcggacgt tgatggctca
    16621 attcgcgcaa cagggcggtt tgtttttgct cttcgttcag gcggttgtag tcttccaagc
    16681 ctgcgtgttg gaaaagctcg gcaaccacat cggcgtgttt gcctgcgtgt tggcgcaagt
    16741 cgagcggcat catgtggaag ccgaacacgg acacggaacg gatgaggtct gccaaacggc
    16801 cttcggcaag caggcggctg ccgttgtcga taagggaacg ttgcaatttt ttcaaatcat
    16861 cgagaaattc ttgtgccgaa gcatacggct cgagaaagcc gaatttgcag cccatgccca
    16921 aaccgagcgc gcgcgctttg cccatagcgc gcgccataat gtaggcgatg gcgcggcggt
    16981 agggttcttc cgtgcgagcg atttcttcgt caggcgagag ggctgccagt gccattacgt
    17041 cgtcgttgac tttgacgcgg cggatggaga gcggcagttc gcggtaaagt ttgtcgagtt
    17101 cgccacggta aaaacggaac actgcgtcgg cgtggcggcg gaaggcaaag cgcagggttt
    17161 cggcagagac gaacgggttg ccatcgcggt cgccgccgat ccagccgccg attttcagga
    17221 tatcgggaac gtccacatcg ggataggccg tctgaaagtc gtgttccatc ttgcggtaga
    17281 gcttgggcag ggcttcgaaa aagctcatcg ggaagatgga cacgccgttg ttgatttcgt
    17341 cgttgacgct gagtttgtgg cggcgcgttt cgctggtctg ccacaagccc agcaggatag
    17401 tgtcgatttc gcggcgcagc cgtgccagcg cgtcggcatt ggtgcagcgt tcgcgttgcg
    17461 gcaacagtgc gcggatgcgg cggttgaagc ttaagacggt ttggcgttgc acttcggtcg
    17521 ggtgcgcggt caaaacggcg gtaacggacg tattgtccaa ctgccgctgc accgatttgc
    17581 cgtcggcttt ccccgctttg agcctgcgga cggtttccgt caggctgcct tccgcgccgc
    17641 cgcgtccggc ttcttcgtgg atttggcggc ggcgttcgtg gtgcacgtct tcggcgatgt
    17701 tcaaaatctg ggcgaacagg ccgcaggcca aggttaaatc gtgggtttgt tgttcgtcca
    17761 attgcggcaa tactttttca atcaatgccg cgctgtcgtc ggaagtggac aagagtttga
    17821 ctgtttcgac aaccaacggc gaggcttctt cgtgcaggag gttgaacagg gattgtttca
    17881 gaaattccgc gtccgccgcc aaagccgcgt ccttcggatt gttcagaata tgcagttgca
    17941 tgatttttct ctcattgccg taaatactgt aaatgtacct caaatgccgc atccgtgcca
    18001 aaccgttcac actttaaccg cccgtgtccc gaaatgccgt ctgaagttga acgccgcccg
    18061 acggcagcgt tacaatcgcc cgcaactgtt ttttccgaac atcatcatga ccacgaccga
    18121 acacgacaac gacgacgaac tcctgctgcg gtacagccgc cacatcctct tggacgaaat
    18181 cggcatcgaa gggcagcaaa agctttccga cgcacatatt ttggtcgtcg gatgcggcgg
    18241 tttgggtgcc gccgcactgc cctaccttgc cgcttcgggt gtcggcacgc tgaccatagc
    18301 cgattccgac acggtcgagc tgcacaacct gcaacgccaa gtcgcatttg acgagggcga
    18361 tgtcggcaag cccaaagccg aagccttggc aggccgtctg aaacgcatca accataccgt
    18421 caacgtccgc gccgtcaacg aaaaactcga cggctgtcgc ctaaccggtt tggttcaagc
    18481 cgccgacatc gttttagact gctgcgacaa ctacgccacg cggcaggcgg tcaaccgtgc
    18541 ctgcgtgcag acgaaaacac cgctggtttc aggggcggcg gtgcgctttg aagggcaact
    18601 tgccgtgtac cgtcccgact tgcccgactc gccgtgttac gcctgcctgt ttgacggcgg
    18661 atcggcttca gacggcatct gttccctctt cggcgtgttc tcgccgctgg tcggcatcat
    18721 cggcagtacc caagcggcgg aagctctgaa aatcctgctg gatgcgggcg aaccgtcgca
    18781 cggcaggctg gcggtttacc gcgccttgga agggggctgg caatatttcg acctgccgcg
    18841 caacccagaa tgcccggttt gcggcgcgga acggtaaaac cctgccgccg tttcagacgg
    18901 catccaaacg gatgcggagg aacggtttta aaaatttaaa aatttacatt tctttgcaaa
    18961 aaaaaaaaaa aaaaaataaa cttaccttat aattgcagtt gttttagcaa tgtctgtttc
    19021 gcagactcat tgagtaagac gttttccccg taatgtgttt ggccgtctgt cccctttggg
    19081 ttcggacggc ttttttttgg ctgtgtatga atacccggtt ggttttatct gtttacaggg
    19141 gaaaaccgct tatttccgtt cgggcggaaa acggttccat cggataaaag gcattttgtc
    19201 cgactgatta aagttatatc ttaggtttac atcggcggaa tattcaaaca cagccttttt
    19261 taaggaaatc cggatacggc ggcgcatcaa taatgcggcg gaatctcgtc gcgcagggaa
    19321 tacggctctt gcgcgtcggg attcctgtcc tgcatttttt gatacagcag cctcaactga
    19381 gcctgctgca aatccagcgt ctgccgcaat tccgccacca tcgcgttcag gccggcgatt
    19441 acgtcctcct gaagcgcgga ttggatttcc aattcgacaa tacggcgttc caactcttga
    19501 accgcgtcca tttacagcac catcgcggca atccagccgg caatcagcag cgggatgttg
    19561 tagtggatga aggtcgggat aacggaatcg cggatgtggt cgtgctgccc gtcggcgttc
    19621 agccccatcg tcggacccag cgtggaatcg gacgcgggcg aaccggcatc gcccaacgcc
    19681 cccgccgtgc cgacaatggc gacggtggca agcggcgaaa aacccaaacc gacacacaaa
    19741 ggcacataaa tcgcggcaat aatcggcaaa gtggaaaagg acgaaccgat gcccatcgtt
    19801 accaaaagcc ccaccaccag catcgccaat gccgccatac ctttgctgtt gccgaatatc
    19861 gccatactgc tttccaccag cggctgaata tgcccggtcg cattcatcac ggcggcaaaa
    19921 ccctgcgcgg caatcataat gaagccgacc atcgccatca tcttgatacc ttcgccgaat
    19981 acgtcgtttg ccttgtcgcg gttgatgacc cccaacatca taaatacggc gaaaccgagc
    20041 atcgcgccca acaccagcga gtcttcatac atcaactgga tggcaaagca tacggcaatg
    20101 gcggcggcgg cggccaggct gcggtaggcg gacggctgcg gacggtttgc cgcatcggcg
    20161 ttgcccgccg tatcggcatt gttgctttgg tacaggcgcg gtttgcggta atggacaaac
    20221 gccagcagga gtccggccag cattcccaac gcgggaatcg ccattgccgc catcacgtta
    20281 atgtttttca catcaagctg cggcgcggcg gaatggatgt tgcccaacag gatttcgttc
    20341 aaaaaaatcg cgccgaagcc gtaaggcagg aacatataag tcgtaaccag cccgaaagtg
    20401 atgacgcacg caatcaggcg gcggtcgatt ttcaggcggt tgaacaccaa aagcagcggc
    20461 ggaacaatca tcgggataaa ggcaatgtgg atggggatga tgttctgact catcatgccc
    20521 atcacaagga tgatggaaag cagcagccat ttgaccgcgc cctcgcccga acgcacgctg
    20581 tcgggcatac cgccccggtt cagtttgcgg acgaccgcgc cggcaagctg ctgcggcagg
    20641 ccggaatggg taatcgccat cgcaaacgca ccgagcatcg cataagaaag cgcaatcttc
    20701 gcaccacctt ccaaaccttt gttaaacacg gggataatcc ccgcctgact gacctgtccc
    20761 gccgcatcgg caatgttttg cagcggcata cccgccaccg cgccgccgac aaacgcgccg
    20821 atcgtcaggc tcaataccac gtgcacgcgc gacagcgaca gcaccagcat aacgattacg
    20881 gcaactacga ctgcattcat tgtttatcgc tccaaaccta taaatgttta catatcgaaa
    20941 cacatcataa cccaataacg ggaaacccgc caattttgca aacaattatt tcaaatgctt
    21001 catatacttc cccagcgtaa ccctgtccaa acccgccaaa tccggcaggg tttccactcc
    21061 tgaaaaacca ttctccgcca acacgccgcg caccgccgcg ccctgatcga aaccgtgttc
    21121 cagcaataaa aaaccgcctt ccgccaaacg gtcgggcgcg ccttgcgcca aggtacggat
    21181 gcagcttagg ccgtctgaaa agtcggtcag cgcgatttgc ggctcaaacc gcaaatcgcc
    21241 ttgcaacaaa tgtttatcgc cgttttcgat atagggcggg ttggacacga tgatgtccca
    21301 tttcccttca gacggcatat cggtgtcgaa ccacgaaccg tgtgcaaatt cgacccgcgc
    21361 gcccaaatcc gccgcatttt cccgcgccgt ttcaagggcg ggcgggctga tgtcggatgc
    21421 gcgcacaaac gcatcggggc gttcgagcgc gacggttacg gcaaccgcgc cgctgcccgt
    21481 ccccaaatcc cacacgcgcc cgttttcggg caggcgcgcc aatacggctt cgaccaaatg
    21541 ttcggtttcg gggcgcggaa tcagcacgct cggattgact gtaaagcgtc tgccataaaa
    21601 ttcgcgcaca cctaaaatat aggcaaccgg ctcgccgttc agacggcatt gcgccagcct
    21661 gtccgcccgc tgtcggactt cgtccggcat ttcttccccg ccccgcgtca acaactgcac
    21721 gcgcgtatat tccgaaacat attgcagcag cattcttgct tcaattttag gcagttttga
    21781 caagcccaac catttatcaa acgtcatttt ttatcccgtc tgccgccggt gcggcttttc
    21841 tttccttatt ctttccggca aacgtaccga tggtggcaac cgcaaatgcg gcataccaca
    21901 aataaaatcc tgcaccgtag cgcacaatat ccgatgtatt ccctgcttca tcgacgtata
    21961 cggctttcac actgaaagcc accaacgcca agccccaaag tgccgcatgg acaggcacga
    22021 ccttcttccg caacgccagc aaaacaatgg ccgccaacca aacataattc gcatagaccg
    22081 cacaatacct gatatccaaa gaagcaaata tcgaccccaa aatcaaaacg gtcaaaccct
    22141 ccatgcttcc atgattgccc aaataaaatg caacattgga taaagacgct atccacaggg
    22201 caaccgacac cagcaacatc actatgggaa aacttgattt ccgattctgt tcctgcatgg
    22261 ttttatccta atgtaaaagg ccgcctgaaa acctttcaga cggcatcgtg ccggattccg
    22321 cgtcagattg cgctgccgcc gacggtcagt ccggcatcaa tccgcaaagt cggttgcccc
    22381 acgccgacgg ggacgctctg cccttctttg ccgcacacgc cgacaccgct gtcgagcgcg
    22441 gtatcgttgc ctatcatcga aacgtgtttc agcacttcgg gaccgttgcc gataatggtc
    22501 gcgcctttga cgggatattg cagcctgccg ccttccaccc accatgcttc ggacgcgctg
    22561 aacacaaatt tgccgctggt aatgtccacc tgtccgccgc cgaagttgac ggcgtaaatg
    22621 cctttgtcga tggacgcgat gatttcttcc ggctcatagc tgccgttttc cataaaggta
    22681 ttggtcatgc gcggcatagg ggcggaggcg tagctttcgc ggcggccgtt gccggtcgat
    22741 tgcgtacccg tcaggcgggc gttggtttcg tcctgcatat agccggtcaa aataccgtct
    22801 tcaatcaata cggtgcggcg ggtttcgttg ccttcgtcgt cgatgttgag cgaaccgcgc
    22861 cggccggcaa tatcgccctg atcgacgacg gtaacgcctt tggcggcgac gcgctcgcct
    22921 attctgccgg aaaagacgct ggtccccttg cggttgaaat cgccttccaa accgtgtccg
    22981 accgcttcgt gcagcaacac gcccggccag ccgttgccca aaacgacggt catttcgccg
    23041 gcgggcgcgg ggcgggattc gaggttggtg agtgcctgtt tgacggcggc atcgacaaac
    23101 tgccgcacca agttttcatc gaaataagcc aagtcgtagc gtccgccgcc gcccgcgctg
    23161 ccctgttcgc ggcgttcgcc ctgtttggcg ataacggtaa cgttcaggcg caccatcggg
    23221 cggatgtcgg cggcgtgttt gccgtccaga cgggcgaggt aaaccatatc gtattcgcag
    23281 gtcaaaccgg ccatcacttg cacgatgcgc ggatcggcgg ctttggcgat tgcttccact
    23341 ttgttcaaca gcgcgacttt ggcggcggaa tcgaggccgg caatggggtc ggacgcggaa
    23401 caaaccggct tgccgcgcgt ttcagacggc attttggcgg acaccttgcc gcctgccgcc
    23461 ccaatcgcgc ggacggcgcg ggcggaacgg tttatcgaat cgatgcacag gctgtcggcg
    23521 taggcaaagg cggttttatc gcccgaaacg gcgcgcacgc ccacgccctg attgatttgg
    23581 aagctgcccg atttgacgat gccctcttcc aaatgccagc tttcataagc ggtgcgctgg
    23641 cagtagatgt cggcgtaatc gacgtggtgc gcgccgatga tgcacaggct tttggcgagc
    23701 agttcggggg aaaggcggtt ggcttcgagc agccgcgcct gtacggcgga ataggtcgga
    23761 tgcatagtgt cggcgcataa aaaatcaggg gcttgattat acggcatttg ttatatagtg
    23821 gattaacaaa aaacagtacg gtgttgcctc gccttgccgt actatttgta ctgtctgcgg
    23881 cttcgtcgcc ttgtcctgat ttttgttaat ccactataga aatgcgccgt gccgccgaaa
    23941 atgtaagaat tttgccaacg cacgttgctt tgtgtaaact taaagctttc ttgtcggagt
    24001 gccgccgccg ggcggctgag attgcgaaag cagaatccgt agaacctgtc ggggtaatgc
    24061 ctgcgtagga aacaaaccgt caaatgcctt atcaggcttc cgttcccttt tccgcacttc
    24121 cccgcccacc ttccttcttt ttaaaggacg tcatatgtcg ggcaatgcct cctctccttc
    24181 atcttccgcc gccatcgggc tgatttggtt cggcgcggcg gtctcgattg ccgaaatcag
    24241 cacgggtacg ctgcttgcgc ctttgggctg gcaacgcggt ctggcggctc tacttttggg
    24301 tcatgccgtc ggcggcgcgc tgttttttgc ggcggcgtat atcggcgcac tgaccggacg
    24361 cagctcgatg gaaagcgtgc gcctgtcgtt cggcaaacgc ggttcagtgc tgttttccgt
    24421 ggcgaatatg ctgcaactgg ccggctggac ggcggtgatg atttacgccg gcgcaacggt
    24481 cagctccgct ttgggcaaag tgttgtggga cggcgaatct tttgtctggt gggcattggc
    24541 aaacggcgcg ctgattgtgc tgtggctggt tttcggcgca cgcaaaacag gcgggctgaa
    24601 aaccgtttcg atgctgctga tgctgttggc ggttctgtgg ctgagtgccg aagtcttttc
    24661 cacggcaggc agcaccgccg cacaggtttc agacggcatg agtttcggaa cggcagtcga
    24721 gctgtccgcc gtgatgccgc tttcctggct gccgcttgcc gccgactaca cgcgccacgc
    24781 gcgccgcccg tttgcggcaa ccctgacggc aacgctcgcc tacacgctga ccggctgctg
    24841 gatgtatgcc ttgggtttgg cagcggcgtt gttcaccgga gaaaccgacg tggcaaaaat
    24901 cctgctgggc gcaggtttgg gtgcggcagg cattttggcg gtcgtcctct ccaccgttac
    24961 cacaacgttt ctcgatgcct attccgccgg cgcgagtgcg aacaacattt ccgcgcgttt
    25021 tgcggaaaca cccgtcgctg tcggcgttac cctgatcggc acggtgcttg ccgtcatgct
    25081 gcccgttacc gaatatgaaa acttcctgct gcttatcggc tcggtatttg cgccgatggc
    25141 ggcggttttg attgccgact ttttcgtctt gaaacggcgt gaggagattg aaggctttga
    25201 ctttgccgga ctggttctgt ggcttgcggg cttcatcctc taccgcttcc tgctctcgtc
    25261 cggctgggaa agcagcatcg gtctgaccgc ccccgtaatg tctgccgttg ccattgccac
    25321 cgtatcggta cgccttttct ttaaaaaaac ccaatcttta caaaggaacc cgtcatgacc
    25381 cgtatcgcca tcctcggcgg cggcctctcg ggaaggctga ccgcactgca gcttgcagaa
    25441 caaggttatc agattgcact tttcgataaa ggctgccgcc ggggcgaaca cgccgccgcc
    25501 tatgttgccg ccgccatgct cgcgcctgcg gcggaagcgg tcgaagccac gcctgaagtg
    25561 gtcaggctgg gcaggcagag catcccgctt tggcgcaaca tcagaggccg tctgaaaacg
    25621 cctgccatga tgcaggaaaa cggcagcctg attgtgtggc acgggcagga caaaccttta
    25681 tccaacgagt tcgtccgcca tctcaaacgc ggcggcgtag cggatgacga aatcgtccgt
    25741 tggcgcgccg acgacatcgc cgaacgcgaa ccgcaactcg gcggacgttt ttcagacggc
    25801 atctacctgc cgaccgaagg ccagctcgac gggcggcaaa tattgtctgc acttgccgac
    25861 gctttggacg aactgaacgt cccctgccat tgggaacacg aatgcgtccc cgaaggcctg
    25921 caagcccaat acgactggct gatcgactgc cgcggctacg gcgcaaaaac cgtgtggaac
    25981 caatcccccg aacacaccag caccctgcgc ggcatacgcg gcgaagtggc gcgggtttac
    26041 acacccgaaa tcacgctcaa ccgccccgtg cgtctgctcc atccgcgtta tccgctctac
    26101 atcgccccga aagaaaacca cgtcttcgtc atcggcgcga cccaaatcga aagcgaaagc
    26161 caagcccccg ccagcgtgcg ttcagggttg gaactcttgt ccgcactcta tgccgtccac
    26221 cccgccttcg gcgaagccga catcctcgaa atcgccaccg gcctgcgccc cacgctcaac
    26281 caccacaacc ccgaaatccg ttacaaccgc gcccgacgcc tgattgaaat caacggcctt
    26341 ttccgccacg gtttcatgat ctcccccgcc gtaaccgccg ccgccgtcag attggcagtg
    26401 gcactgtttg acggaaaaga cgcgcccgaa cgcgatgaag aaagcggttt ggcgtatatc
    26461 cgaagacaag attaaagccg tctgaaagga accccatgac cttcctgcca ctcaaatccc
    26521 cgctcaaatt ctacgccgtc gtccccaccg ccgattgggt cgagcgcatg gtcgaagcag
    26581 gtgccgacac ggtgcaactg cgctgcaagg ccctgcacgg cgatgaattg aaacgcgaaa
    26641 tcgcccgctg cgccgcagcc tgccgtggca gccgtacgca gcttttcatc aacgaccact
    26701 ggcgcgaagc gatagaagca ggcgcgtacg gcgtgcatct cggacaagaa gacatggaca
    26761 ccgccgacct cgccgccatt gccgctgccg gtttgcgctt gggtttgagt acgcactccg
    26821 tcgccgaact cgaccgcgcc ctgtccgtgc accccagcta cgtcgccagc ggcgcgattt
    26881 tcccgaccac gaccaaacaa atgcccaccg cccctcaagg cttggaaaaa ctgcgtgaat
    26941 atgtgaaaca ggcaggcagc acgcccgtcg tcgccattgg cggcatcgat ttgaacaacg
    27001 cccgagccgt actcgccacc ggcgtttcct cactcgccgc cgtccgcgcc gtaaccgaag
    27061 cggcaaatcc cgaagcggtg gttaaagcgt ttcaggcttt gtgggatgga taaaaccgaa
    27121 agaagaaaat tcaattgccg tgtcggcaaa acttagcccg ttatcgcaaa catacttaac
    27181 tttaaatgtg gcatatcatc aaattccgtc attcccgcgt aagcgggaat ccgccttaaa
    27241 acttgagaaa ccatcatttg aaaaacagtt tccgaatttc aaaaatggat tcccgcctgc
    27301 gcaggaatga cggcaaccgg ccggttgcgt atcaaaaaat aaagtaattc ggctagatat
    27361 agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa atagtacgga
    27421 accgattcac ttagtgcttc agcaccttag agaatcgttt tctttgagct aaggcgaggc
    27481 aacgccgtac tggtttttgt taatccacta taaatacaga aacatcgaga aaccatgaac
    27541 atcatcttaa acggcgaacc cgccgaactt cacggcacaa gcgttgccga cctcatcgcc
    27601 caaaccgcgc cgcaaaagcc ctttgccgtg gcggtcaaca ccgttttcgt ccccaaaggc
    27661 gcgtatgcgg aaacggtttt aaacgaaaac gacaaaatcg atatcgtgcg gccggtggtc
    27721 ggcggctagg cggttttgcc ttttcagacg acccctgtcc ccaaaacaac gttatggtgg
    27781 attaacttta aatcaggaca aggcgacgaa ccgcagacag tacaaatagt acggaaccga
    27841 ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc
    27901 cgtactggtt tttgttaatc cgctatacaa aggaacccat tatgctcacc ctatacggag
    27961 aaactttccc ttcacggctg ctgctcggca cggctgccta cccgacccct gaaatcctca
    28021 aacaatccgt ccgtatcgcc caacccgcga tgattaccgt ctcgctgcgc cgcacgggaa
    28081 gcggcggcga ggcgcacggt caggggtttt ggtcgctgct tcaagaaacc ggcgttcccg
    28141 tcctgccgaa cacggcaggc tgccaaagcg tgcaggaagc ggtaacgacg gcgcaaatgg
    28201 cgcgcgaagt gtttgaaacc gattggatta aactcgaact catcggcgac gacgacacct
    28261 tgcagccgga tgtgttccaa cttgtcgaag cggcggaaat cctgattaaa gacggcttca
    28321 aagtgctgcc ttattgcacc gaagacctga ttgcctgccg ccgcctgctc gatgtgggct
    28381 gtcaggcgtt aatgccgtgg gcggctccga tcggcacggg tttgggcgcg gttcacgcct
    28441 acgcgttgaa cgtcctgcgc gaacgcctgc ccgacacgcc gctgattatc gacgcgggct
    28501 tgggtttgcc ctcacaggcg gcacaagtga tggaatgggg ctttgacggc gtgcttttga
    28561 acaccgccgt ttcccgcagc ggcgatccgg tcaatatggc gcgcgccttc gcactcgccg
    28621 tcgaatccgg acggctggca tttgaagccg aaccggtcga ggcgcgaacc aaagcgcaag
    28681 ccagcacgcc gaccgtgggg cagccatttt ggcattcggc ggaatattga aaaaggcggc
    28741 aaaaatgccg tctgaaggct tcagacggca tcgcggctca aaacggcggc agcctgaaac
    28801 ggacaaaccg ccattccccg gcatcacggc tttgtcggta aaaatggaaa aaccggccgg
    28861 aaaaccttgc cgcccacccc gacaccgcaa ccaaagaaac acccagactc cacggtgtgc
    28921 aggctgccgc gcaagcccta aatacggcaa tagaagaagc gttttgttgt tgtttgaata
    28981 cacttaaaaa tacctaaagc cgtccgtaag ccttcatccg caacggtttt accgctttcg
    29041 catccccgaa tccacgctca aacacccccg aatgacaacc ctgtccgcgc ccaatcggac
    29101 ggatgttcaa acacgggcaa ccttatttcc gtcaggcacg aagccctcag ctatgactgc
    29161 cgaccccgat ttgtccgaca caatgaaagt ttgccgaccc gaatcacaaa catcggcgga
    29221 caggttaatt tgtttatttt tcatcgtatt acaaaaaatc tgcatttatt tttaaatttt
    29281 tattgataat tattattatt agcgtataat caaaaccact cggaagccgt ccgttccgaa
    29341 ccattaaaca ccatatttcc ccatcatcac tttcacactt ggagtcggca tatacgagac
    29401 atacattccc tttttatata tcagatactc aaaaccgaaa cgccaaaccc accttcgcgg
    29461 tgggtttggc gtttatcgtc cggctttcgc gcctatttgc aagacttgag gttcagtttg
    29521 ccgtataggg acgtgatttt acgaatttcg tccgcatcgg cggcattcac gccggtaaac
    29581 aaaaccgtca tacgcgacac gctcaaagaa tcgtcctgcc tgtcggcttc ggcaaagtgt
    29641 tcgacaatat gcgccccgcc gaatccggcg ccggcaagcc ggttttgcag ggcttgggcg
    29701 ttttcccggc tgttgccgac acccaaactc aatgcgccgt caaacggtat ggggttgaaa
    29761 cctttggcag acagctccgc cgcctgattt tcggcatcgg cggaaacggg caggacgacg
    29821 cggtaggttt tgtcggcagg tttggctttg gcggtgcgtt tttcgacgct cctgctggca
    29881 acgtgcgacc atttgcccaa aagtcctttg atgcggtggt agtcatcttc gtccatcgtg
    29941 aggcttgcct gcgcggcgca taaggcatcc gccgcgccgt tttcgcgttc cgcctgcgcc
    30001 ttttcggcgg cgagtttttc gcggcgggct ttttcttcac gctgtttttt ctccctcagt
    30061 tttttctgtt ccgcttcctt tttcaagcgc aactgctcgg cctgctcttc ggtcaggatg
    30121 tcgcccggtt tgagcagtgc cgccgtatcc tccacggatg ccgccgcatt gtcggcggcg
    30181 ggcgttgcgg ggggtgtgtt ttcgacgacc cggttttccg gcacgccgcc cgtcgcttta
    30241 acggccaatt tatagcccac cgtgccgccg aatacggcaa tattgagtac cgccaaaagg
    30301 gtgaagagcc atttcattag aatttcctca atgtttgaaa ccccgcccgc ctgggcggca
    30361 gtctgtcggg cggcagaatc cggacggatt cggacggatg ccgccccgtg ttgaaaaagg
    30421 gtgtattttt taagtatgtt ccgattcccc gccttcggcg gcaatcaggt tcagcagccc
    30481 gtgaatgacg aggttgtccg ccacgcgcac ggtattttcc gccaaaaatg caggcggcag
    30541 ggcttcggca acttttgccg cgccgccgcc ggtaatgatg acatcgacag gcttgcccgc
    30601 cccggttttt tctttcaaac gcccgtgcat catcataacc gagccgcaaa ccgcatccat
    30661 catgccgctg gcgacggcat tgcccgttgt ggtcgggaaa ggataacgct taccggcgtg
    30721 ccggttgagg ttggcggttc ggacggcgag cgattctttc atcaggtgga aaccgggcat
    30781 gatggttccc ccgagataat gtccgtcatc ggtgagcgcg tcaaccgtta ccgccgtgcc
    30841 gcaactgacg acgacgcagg cattgcggct gaagcggcgg ctgcccaagg cgttgaacca
    30901 gcggtcggaa ccgtgttctt cggggtggcg gtagtggttg cgtatgccca aagcctgtgc
    30961 ggaagacggc agccactcga tttttcgggc gagctgttcc tgcacttgtg cctttttgaa
    31021 ttctccgcac acggcgcaac cgacgatgcg gacatttcca tccgcctttt ccgcccactc
    31081 cgcgcccaaa ggcgacaaat cgcggtacgg cgcgctaccg acggttgcga acgtgccgtt
    31141 ttccacccac gcccacttga gccggctgtt gccgccgtcc aacagcagaa aacgttccga
    31201 atcccgccgc ttcggcacgg aaaccggcct gtcgtcggac cgcaggctga tttcgccgct
    31261 gacgaccgtc tgtttgccct ctgccgtttc caagtgcaaa acgccttgtc cgtccacgcc
    31321 tttaaccgtg ccttcgaaca cggtttcgcc gtcgcgcaac agcaataccg ccttgccgtg
    31381 gtcgcggttg gcagcctgat attccgccac aaaaggcgca aatccgtccc gcgcatattg
    31441 caacaacacc gcatcaagtt ccgccaacag cgtttccagc aacacggcgg catcggcatt
    31501 tccccgccgc gatgccgtct gaaacagcga ttgcacggaa gcggcatttt ccacttcctt
    31561 gggcaggaca aaattgatgc cgataccgac gacggcaacc gtcttgccgc ccgccctgac
    31621 cgtttcaatc agaatgccgc ccaatttatc gcgccccacg accaaatcgt tgggccactt
    31681 gatttgcgtt ttcaaaccca aacgcgacaa ggcgcgccgg cacgccactg ccgcaacagg
    31741 cgacagcgaa cccaactcat actgcggccg gtcaaacacc cagccaaaac tgaacatcag
    31801 acactcgccc aaacggtgcg accacttccg cccctgccgc cccctgccct tactttgcag
    31861 gtgggtcaca catatggttt tgtgcgcctt gtccggcgca atccgcgcca attccagtat
    31921 ctcgtcgttg ctggacgcgc actcgtgctt caatgccgtc tgaaaacccg acctttcccc
    31981 cagctcgcgc aaaccttcgg catcgaaaac cgccaatggg cgcaccagcc gccaatagcc
    32041 gtcgtgttgg cgcaacagcc cgcgtatgtg cgccggcatc tgctgccaaa aaccgttgag
    32101 ctgctgcggc ttcatatccg ccatacgcgc cagttgcgag acgtgttgcg gcaaaccgtc
    32161 ggcaagctcc gccaacaccc gccagtgcga aagcttcaaa accgtcattt tccgccctct
    32221 gccgcacgga tttttgccaa agtcttcgtt gtcgaagtct ggtgcagaaa cggaattgaa
    32281 aacacctgac cgccgcgcgc caacgtttct gccgcaccga caatcttatc cgcagcccaa
    32341 tcgccgccct tgaccaaaat ctcaggtttg accgcctcaa tcaacgccgc cggcgtatcc
    32401 ccgtcaaacc acgttaccaa atccacactt tccaaagcgg cggcaacggc ggcacggttc
    32461 tccaaaggat taatcgggcg gtcgccgccc ttgcccaaac gccgcaccga agcatcggta
    32521 ttcaacgcca gcaccaacgc gccccccatc gaacgcgcct gcgccagata agtaacgtgc
    32581 cccctgtgga ggatgtcgaa acagccgttg gtaaacacca gcgggcgcgg caacaacgcc
    32641 aaacgcgccg ccaacgcctc gggcggacag attttcgatt caaaatcagg gacagaccaa
    32701 gcgtcaacca tcaaagcctc cgacaaaaac cataaaagac agaaaaaccc acatgataca
    32761 gaagcatatg cgaaaggcaa agccggcggc gcggacagta cgcgcaaacg agaaaagacc
    32821 cgtaccgaaa agtacgggcc tttatctggg gtggctgatg gggctcgaac ccacgacaac
    32881 cggaatcaca atccggggct ctaccaactg agctacagcc accataaaaa cggttttcaa
    32941 tcaaattctt ggcacgcccg acaggaatcg aacctgtaac ccccgactta gaaggtcggt
    33001 gctctatccg gttgagctac gggcgctcat gccgattcgt gctgattgat tggtcggggc
    33061 ggtgggattc gaactcacga ccctctgctc ccaaagcaga tgcgctaacc gggctgcgct
    33121 acgccccgac ttgaagaagc gaactataca actcagggaa agatgcgtca atatttattt
    33181 tcaagacacc aagatgaaaa atatagtttt ttgatttgaa aaaatattta atccgtccaa
    33241 acagccgtat tttatttcag ggcaaattta ttttcggcat cctgctgtaa aaacaaacgg
    33301 aaaatgcgat aattttcagc attttctacc tgtttaacaa aaggacggat atgtcggcac
    33361 aactgatcaa tggtaaagaa gtttcgcaaa aacgcctgca ggcggttgcc gaagcggtgg
    33421 cgcaacgcca acagaacaat ctgcacacgc cttgcctggc cgtggttttg gtcggaggcg
    33481 accctgccag cgcggtttat gtccgcaaca agaaaacctc ctgccaaaaa tgcggcatca
    33541 aatcactgtc ttacgagctg cccgaatcaa catcgcagga agaactgctg gcactggtcg
    33601 accgcctgaa tgtcgattcc gaagtggacg gtattctggt tcagctgcca ctgccgaagc
    33661 acctcgacag ccaggcggtt ttggaacgta tttcgccgga taaggacgtg gacggcttcc
    33721 atccttacaa tgtcggcagg ctggcggtca aaatgccgct gatgcgcccg tgtacgccca
    33781 agggcgtgat gacgcttttg gaggcttacg gcatcgatcc gaaggggaaa aaagcggtcg
    33841 tggtcggcgc gtcgaatatc gtcggccgcc cgcaggcgtt ggaattgctg ctggcgcgcg
    33901 caacggtaac ggtctgccac agcgcaaccg aaaatctggc agacgaggtt gccggagccg
    33961 atattttggt ggtcggcgtg ggcattccga actttgtcaa aggcgaatgg atcaaacccg
    34021 gcgcggtcgt tatcgatgtg ggcatcaacc gtttggacga cggcagcctg tgcggcgacg
    34081 tggaatttga aacggcaaaa gaacgggcgg cgatgattac gcccgttccc ggcggcgtgg
    34141 gtccgatgac gattgccaca ttgatggaaa acaccctgca cgcggcttca ctgcacgatg
    34201 cttgagcggt tctgaagata aaaatgccgt ctgaaaggct ttcagacggc attttgccgt
    34261 gtccgtttat ttgggcagct tgacgacaac cgtatccgcc agtatgtcgt aaagcgtgcg
    34321 gcggtcgcgt ttgaccataa agagcaggac aaagttggca aggaatgcca gcaggttgat
    34381 ggcgttttct ccgttgtcac ctactgcaag accgataacg gcggcaataa tggcaaccaa
    34441 aaccgaccat gcgatttcgc gtaccaaaac cgtgccgaca aaacccggat tacggccgtc
    34501 ggttttcaac acacggattc tcatgatttt cttacccaat gactgcccgt cccggctcat
    34561 atagtagatt tgaacgacgg tataaaccaa aatgcccgcc agtcctaccc aaaaggaagt
    34621 catgcccaaa agcagcccga atatttcctc actgccgcca atcctgcctt cattcttgat
    34681 ggcgaaagca atcagtccgg caaacggcac caacaaaacc aaaaaggtaa acaattggtt
    34741 cagcagcgcg gcaagtatcc ggtcgcctgc gccggcaatt ccgacttcaa tttcctgccc
    34801 gttgcggttg tcggatgccg cgtcggtgta gtcgtttttt tcttccatat ccgttcctga
    34861 taattgttct taactgaccc cgattctacc gccacgacac cgaaaacgcc aatacttaaa
    34921 gaaatcccga taaagaactt tacattttcc caatacggcg ttaaaacgct tcctttacgc
    34981 catacata
//
LOCUS       DAWFHY010000012        34520 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.012, whole genome shotgun sequence.
ACCESSION   DAWFHY010000012 DAWFHY010000000
VERSION     DAWFHY010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 34520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34520
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.012"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            80..1045
                     /locus_tag="WGQ68_000495"
     CDS             80..1045
                     /locus_tag="WGQ68_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0714357.1"
                     /translation="MLRLTALAVCTALALGACSPQNSDSAPQAKEQAVSAAQSESASV
                     TVKTARGDVQIPQNPERIAVYDLGMLDTLSKLGVKTGLSVDKNRLPYLEEYFKTTKPA
                     GTLFEPDYETLNAYKPQLIIIGSRAAKAFDKLNEIAPTIEMTADTANLKESAKERIDA
                     LAQIFGKQAEADKLKAEIDASFEAAKTAAQGKGKGLVILVNGGKMSAFGPSSRLGGWL
                     HKDIGVPAVDESIKEGSHGQPISFEYLKEKNPDWLFVLDRSAAIGEEGQAAKDVLNNP
                     LVAETTAWKKGQVVYLVPETYLAAGGAQELLNASKQVADAFNAAK"
     gene            1063..1212
                     /locus_tag="WGQ68_000496"
     CDS             1063..1212
                     /locus_tag="WGQ68_000496"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714358.1"
                     /translation="MPSETRMQTASCVSDGIARYGGFKQGFPLRRFGLPCLNLLRLNA
                     FPFCL"
     gene            1209..2177
                     /locus_tag="WGQ68_000497"
     CDS             1209..2177
                     /locus_tag="WGQ68_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0714359.1"
                     /translation="MTAKPFSLNLTNLLLLAVLFAVSLSVGVADFRWSDVFSLSDSQQ
                     VMFISRLPRTFAIVLTGASMAVAGMIMQILMRNRFVEPSMAGAGQSAALGLLLMSLLL
                     PAAPLPVKMSVAAVAALIGMLVFMLLIRRLPPTAQLMVPLVGIIFGGVVEAVATFIAY
                     ENEMLQMLGVWQQGDFSSVLLGRYELLWITGGLAVFAYLIADRLTILGLGETVSVNLG
                     LNRTAVLWSGLIIVALITSLVIVTVGNIPFVGLVVPNIISRLIGDRLRQSLPAVALLG
                     ASLVLLCDIIGRVIVFPFEIPVSTVFGVLGTALFLWILLRKPAYAV"
     gene            2167..3141
                     /locus_tag="WGQ68_000498"
     CDS             2167..3141
                     /locus_tag="WGQ68_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEZ0714360.1"
                     /translation="MPSENKIEFMQGSPRPLWAAFALLLICCGLFLTLNVKGDWDFVL
                     HLRLTKLAALLMVAYAVGVSTQLFQTLTNNPILTPSILGFDSLYVFLQTLLVFTFGGV
                     GYTSLPLTGKFGFELVVMMGGSLLLFYTLIRQGGRDLPRMILIGVIFGILFRSLSSLL
                     SRMIDPEEFTAAQANMFAGFNTVHSELLGIGALILLVSAAVVWRERYRLDVHLLGRDQ
                     AVNLGISYTRNTLWILLWIAALVATATAVVGPVSFFGLLAASLANHFSPSVRHSVRLT
                     MTVCVGGILLVGGQTVFEHFLGMKAVLSVVVEFAGGLVFLYLVLKHKK"
     gene            3186..3812
                     /locus_tag="WGQ68_000499"
     CDS             3186..3812
                     /locus_tag="WGQ68_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714361.1"
                     /translation="MTQERFPSFFNQAPTITVRDPLAAFLGAAQDGILTYRYADAVRL
                     CGHSCPTVAGAYLMVIKGLKTLYGEELPERGGIEAAMQGARDEGTTGVTASVVQLLTG
                     AAPETGFGGIGIQGRFARRNLLSFGAGEINGTLTLRRKDNGKTVAVSINAALKPFAPE
                     MRELMPKAVGGSASTEELERFGQLWQARVKAFLTESADDPQFVIVREV"
     gene            3900..4658
                     /locus_tag="WGQ68_000500"
     CDS             3900..4658
                     /locus_tag="WGQ68_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714362.1"
                     /translation="MITIRNVSYRIGTRPILDNISLDIPEGGITALIGPNGAGKSTLL
                     SFMARLQPLVHGNISYAGKDIKTTPTAELARTLSILTQENSIMSRITVRDLLMFGRYP
                     YHQGRPSETDKTIVEEALAEFHLQDFADRYLTELSGGQRQRAMIAMVFCQRTDYVLLD
                     EPLNNLDMYHARSLMQILRRLTDEHKRTTVAVLHDINQAAAYADHVVAMKNGKVALTG
                     TPEEVFTVHNIKELFDMDVSVLDHQGKKLIVHHI"
     gene            complement(4717..5055)
                     /locus_tag="WGQ68_000501"
     CDS             complement(4717..5055)
                     /locus_tag="WGQ68_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEZ0714363.1"
                     /translation="MKKIEAIVKPFKLDDVREALTEIGITGMTVSEVKGFGRQKGHTE
                     IYRGAEYAVDFLPKVKIELVLADDAVERAIDVIVEVARSGKIGDGKIFVLPVEEAIRI
                     RTGERADAAV"
     gene            5150..5362
                     /locus_tag="WGQ68_000502"
     CDS             5150..5362
                     /locus_tag="WGQ68_000502"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714364.1"
                     /translation="MPETVIPAQAGIPISAFQQYLKVSVNPNFHIPNRAETTAFDLKT
                     YRSSCPPLYFLQLSAYPALGKNQKNR"
     gene            5519..9481
                     /gene="purL"
                     /locus_tag="WGQ68_000503"
     CDS             5519..9481
                     /gene="purL"
                     /locus_tag="WGQ68_000503"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEZ0714365.1"
                     /translation="MSVVLPLRGVTALSDFRVEKLLQKAAALGLPEVKLSSEFWYFVG
                     SEKTLDAATVEKLQALLAAQSVEQTPKAREGLHLFLVTPRLGTISPWASKATNIAENC
                     GLAGIERIERGMAVWLEGALTDEQKQQWAALLHDRMTESVLPDFQTASKLFHHLESET
                     FSTVDVLGGGKEALVKANTEMGLALSADEIDYLVENYQALQRNPSDVELMMFAQANSE
                     HCRHKIFNADFILNGEKQPKSLFGMIRDTHNAHPEGTVVAYKDNSSVIEGAKIERFYP
                     DAAENQGYRFHEEDTHIIMKVETHNHPTAIAPFAGAATGAGGEIRDEGATGKGSRPKA
                     GLTGFTVSNLNIPGLKQPWEQDYGKPEHISSPLDIMIEGPIGGAAFNNEFGRPNLLGY
                     FRTFEEKFDGQVRGYHKPIMIAGGLGSIQAQQTHKDEIPEGALLIQLGGPGMLIGLGG
                     GAASSMDTGTNDASLDFNSVQRGNPEIERRAQEVIDRCWQLGGKNPIISIHDVGAGGL
                     SNAFPELVNDAGRGAVFKLREVPLEEHGLTPLQIWCNESQERYVLSILEKDLDTFRAI
                     CERERCPFAVVGTATDDGHLKVRDDLFANNPIDLPLNVLLGKPPKTTRSDKTVTPSKK
                     PFHADDIDITEAAYRVLRLPAVAAKNFLITIGDRSVGGMTHRDQMVGKYQTPVADCAV
                     TMMGFNTYRGEAMSMGEKPTVALFDAPASGRMCVGEAITNIAAVNIGDIGNIKLSANW
                     MAACGNEGEDEKLYRTVEAVSKACQALDLSIPVGKDSLSMKTVWQDGEEKKSVVSPLS
                     LIISAFAPVQDVRKTVTPELKNVEDSVLLFVDLGFGKARMGGSAFGQVYNNMSGDAPD
                     LDDTGRLKAFYNVIQQLVAEDKLLAYHDRSDGGLFAVLVEMAFAGRCGLDIDLNLLLA
                     QTFITNHTALSQSLRTEEVKALAEWQETIARTLFNEELGAVIQVRKQDVADIINLFYQ
                     QQLHHNVFEIGTLTDENTLIIRDGQTHLISDNLIKLQQTWQETSHQIQRLRDNPACAD
                     SEFALIGDNDRSALFADVKFDVNEDIAAPFVNSGAKPKIAILREQGVNGQIEMAAAFT
                     RAGFDAYDVHMSDLMAGRVNLADFKMLAACGGFSYGDVLGAGEGWAKSILFHPALRDQ
                     FAAFFADPNTLTLGVCNGCQMVSNLAEIIPGAEAWPKFKRNLSEQFEARLSMVHVPKS
                     ASLILNEMQGSSLPVVVSHGEGRADFALHGGKISDDLGIALQYVDGQNQVTQTYPLNP
                     NGSPQGIAGVTNADGRVTIMMPHPERVYRAAQMSWKPEDWTELSGWYRLFAGARKALG
                     "
     gene            9576..10334
                     /gene="gloB"
                     /locus_tag="WGQ68_000504"
     CDS             9576..10334
                     /gene="gloB"
                     /locus_tag="WGQ68_000504"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEZ0714366.1"
                     /translation="MKITPVKALTDNYIWMIQHGNHAVCVDPSEPSPVLEFLVRNRLM
                     LAQTWVTHPHPDHEGGAAALWRGYMESPVYGESDIEAATHTVTAGTQFTFGDGQVTVW
                     ATPGHTDRHTSYLLETSDGIHVFCGDTLFSAGCGRVFTGTIEQLYDSFQRFNRLPENT
                     LFYPAHEYTAANLDFAAHIEPDNADIQTALKAAAHTPTLPVTLAHERRVNPFLRVDLP
                     HVRDRAEALSGKTLNSSLDTFVALRELKNQYRTK"
     gene            10657..15131
                     /gene="mspA"
                     /locus_tag="WGQ68_000505"
                     /gene_synonym="ausI"
                     /pseudo
     CDS             10657..15131
                     /gene="mspA"
                     /locus_tag="WGQ68_000505"
                     /gene_synonym="ausI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242022.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin/serine protease MspA"
     gene            15363..16817
                     /gene="mgtE"
                     /locus_tag="WGQ68_000506"
     CDS             15363..16817
                     /gene="mgtE"
                     /locus_tag="WGQ68_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEZ0714367.1"
                     /translation="MSIEHTPPTHDGETGQNHAERPSADFDRVHSLCEILEPAFEQIE
                     NGTPLEDAPLRDKLTELTVLLSELHPADVAAVLESLPPRERNVVWLLVKPEDDGEVLL
                     EVSDAVRETLIESMDKDELLAAVDDLDADELAELADDLPHQVVYEALQTRDEEERAQV
                     KAAMSYEDNQVGAIMDFELVSIRADVACEVVLRYLRRFDSLPDHTDKIFVVDENDVLQ
                     GVLPIRKLLVADPEDLVENVMAKDVVRFRAEDDVEEAAQAFERYDLVTAPVVDENKKL
                     IGRITVDEMVDVIREESEADMLNMAGLQEEEDLFAPVWDSVKNRWMWLAVNLCTAFLA
                     SRVIGAFEGSIEKIVALAALMPIVAGIGGNSGNQTITMIVRAMAMGQLTGMQAGRLLK
                     KEVGVALVNGIIWGTVMGAVSWLLYGSLGIGLVMIAAMTLNLLLAATVGVLIPVVMEK
                     FGRDPALGSSVLITAVTDSGGFLIFLGLATLFLL"
     gene            complement(16903..17811)
                     /gene="hslO"
                     /locus_tag="WGQ68_000507"
     CDS             complement(16903..17811)
                     /gene="hslO"
                     /locus_tag="WGQ68_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEZ0714368.1"
                     /translation="MNQTAINRADVRTRFIFDDMPVRGLHVRLENVWQHIVKQKNYPA
                     AIRRALGELLAAGVLLSGNLKNEGTLIVQVQGRGRLKMLVAEAASDRTVRATARWDET
                     AEIADDESLGDLLGEGGVFVLTLQPKDGEPWQGVVPLEGGGIAQMLANYMKRSEQIDT
                     HIVLSASDEAAGGLLVQRLPEAVLDEEAWEHVSTLARTLTAEELAGLDAQHVLYRLFH
                     ETPPRVFEPETFEFSCTCSRGKVSDMLLMLGGEEVGGVVVEQGSIEVDCDFCHSKYVF
                     DETDVNALFGEDVVGVAKGLPRHTVQ"
     gene            18056..18811
                     /locus_tag="WGQ68_000508"
     CDS             18056..18811
                     /locus_tag="WGQ68_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0714369.1"
                     /translation="MDSFFKPAVWAVLWLMFAVRPALADELTNLLSSREQILRQFAED
                     EQPVLPINRAPARRAGNADELIGSAMGLNEQPVLPVNRVPARRAGNADELIGNAMGLN
                     EQPVLPVNRAPARRAGNADELIGNAMGLLGIAYRYGGTSVSTGFDCSGFMQHIFKRAM
                     GINLPRTSAEQARMGTPVARSELQPGDMVFFRTLGGSRISHVGLYIGNNRFIHAPRTG
                     KNIEITSLSHKYWSGKYAFARRVKKNDPSRFLN"
     gene            18830..19048
                     /locus_tag="WGQ68_000509"
     CDS             18830..19048
                     /locus_tag="WGQ68_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714370.1"
                     /translation="MSMPEMPKWYDDDGQIVSCTEKVKVMSENMAELYQTAQDAFEDA
                     LLMGCGERQLRDYLLALIEGLENPYRKV"
     gene            19193..19537
                     /locus_tag="WGQ68_000510"
     CDS             19193..19537
                     /locus_tag="WGQ68_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEZ0714371.1"
                     /translation="MNIIRALLIILGCLAAGETAVFLAGIKLPGSIVGMGVLFALLQA
                     GWVKTSWLQQLTDALMANLTLFLVPPCVAVISYLDLIADDWFSILVSASASTLCVLLV
                     TGKVHRWIRSII"
     gene            19537..20229
                     /locus_tag="WGQ68_000511"
     CDS             19537..20229
                     /locus_tag="WGQ68_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEZ0714372.1"
                     /translation="MNEILRQPSILLFLTLAVYALAIIVRTRTGNIFCNPVLVSTIVL
                     IAYLKILGIDYAVYHNAAQFIDFWLKPAVVVLAVPLYQNRRKIFNQWLPVIVSQLAGS
                     VTGIVTGMYFAKWLGAEREVVLSLASKSVTNPIAIEITRSIGGIPAITAATVIIAGLV
                     GQIAGYKMLKNTVVMPSSVGMSLGTASHAMGIAASLERSRRMAAYAGLGLTFNGVLTA
                     LIAPLLIPVLGF"
     gene            20296..21516
                     /gene="argJ"
                     /locus_tag="WGQ68_000512"
     CDS             20296..21516
                     /gene="argJ"
                     /locus_tag="WGQ68_000512"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEZ0714373.1"
                     /translation="MAVNLTEKTAEQLPDIDGIALYTAQAGVKKPGHTDLTLIAVAAG
                     STVGAVFTTNRFCAAPVHIAKSHLFDEDGVRALIINTGNANAGTGAQGRIDALAVCAA
                     AARQIGCKPNQVLPFSTGVILEPLPADKIIAALPKMQPAFWNEAARAIMTTDTVPKAA
                     SREGKVGDKHTVRATGIAKGSGMIHPNMATMLSFIATDAKVSQPVLQLMTQEIADKTF
                     NTITVDGDTSTNDSFVIIATGKNSQSEIDNIADPRYAQLKELLCSLALELAQAIVRDG
                     EGATKFITVRVENAKTRDEARQAAYAVARSPLVKTAFFASDPNLGRLLAAIGYAGVAD
                     LDTDLVEMYLDDILVAEHGGRAASYTEAQGQAVMSKAEITVRIKLHRGQAAATVYTCD
                     LSHGYVSINADYRS"
     gene            21576..22985
                     /locus_tag="WGQ68_000513"
     CDS             21576..22985
                     /locus_tag="WGQ68_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0714374.1"
                     /translation="MTSTFPRRLARKIRQTRRLSRKSIAFLFLLAGSALVALTALFFA
                     HLADFALELNAKLVQQYPWFAWVALPLGLPLIAWLTRKFAPFTAGSGIPQVIASLSLP
                     YGAQKTRLIRLGQTLLKIPLTFLGMLFGASIGREGPSVQVGAAVMGAWGAWCKKHGLA
                     FKGMQENDLMAAGAAGGLAAAFNAPLAGVIFAIEELGRGIMLRWERQILLGVLAAGFI
                     QVAIQGNNPYFSGFNGGVLEHIFLWVALSGLVCGAAGGLFGRLLYRGAAAFAPRKIRG
                     FIRNRPLLLAALMGLLLALLGTFYQGKTYGTGYHEAAQALHGIYEAPFGLTAAKWLAT
                     VFSYWAGIPGGIFTPSLTIGAVLGEHIAAIADISQGANIIVLICMAAFLAGATQSPIT
                     SAVVVMEMTGGQSLLFWMLIACIFASQVSRQFSPRPFYHASGMRFRQRVLQETAAQTG
                     NAPARPQTANSKTGMPSEN"
     gene            23174..24565
                     /gene="hrpA"
                     /locus_tag="WGQ68_000514"
     CDS             23174..24565
                     /gene="hrpA"
                     /locus_tag="WGQ68_000514"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HEZ0714375.1"
                     /translation="MFPDFSQTLSKDRHFLQSAFKNPNKYGGLSKVEEKYRKSHEIFL
                     RRLAALPKPEFDNTLPVHEKLEEIKKTVAEHQVTIICGETGSGKTTQLPKICLELGRG
                     AAGLIGHTQPRRLAARSVAERIAEELKSEIGSAVGYKVRFTDHTSRDACVKLMTDGIL
                     LAETQTDRYLAAYDTIIIDEAHERSLNIDFLLGYLKQLLPRRPDLKVIITSATIDAER
                     FSQHFNGAPVLEVSGRTYPVEILYRPLTGKDEDDAEVELTDAIVDAADELARHGEGDI
                     LVFLPGEREIREAAEALRKSTLRRNDEILPLFARLSHAEQHKIFHPSGAKRRIVLATN
                     VAETSLTVPGIKYVIDTGLARVKRYSARAKVEQLHVEKISQAAARQRSGRCGRVSAGV
                     CIRLFSEEDFNSRPEFTDPEIVRSNLAAVILRMASLNLGDVAAFPFLEMPDSRYINDG
                     FQVLLELGAVEAA"
     gene            24666..25793
                     /locus_tag="WGQ68_000515"
     CDS             24666..25793
                     /locus_tag="WGQ68_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714376.1"
                     /translation="MKHLLIDFENVQPQNLDKLLTENTHIWLFIGVLHKMLPISLVQS
                     LLRFGERVHLVQLQKTGKNALDFYLSYYLGQITATDPNAQIGILSRDGGYDVLVEHIL
                     KNHQAKGIVRLANIDEVQHQKITTEPPSALLENTPQPETTLKPQQPLTSYFQAALTAL
                     RRPDAFRPCRLHNLRQNLRKHILSDLFKEKTDEECEITTANVINKLKAQNFISIDEQE
                     TVSYHLSDNDLLQRIQRHILSQRPKTYADFQAVVQNRADALHLTVGTNDIQSFARHLR
                     DQNLIRQNNGKIEYAPFTEPKPQPTPKQPKKTAWEPDEIIWKKVIAALSLKNRPNKTK
                     TLRNTIQALTKSNAQETDKLLQHLQDTQVLRIDGTKIVYIK"
     gene            25970..27547
                     /gene="lpt3"
                     /locus_tag="WGQ68_000516"
     CDS             25970..27547
                     /gene="lpt3"
                     /locus_tag="WGQ68_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase"
                     /protein_id="HEZ0714377.1"
                     /translation="MMKKSLFVLFLYSSLLTASEIAYRFVFGIETLPAAKMAETFALT
                     FVIAALYLFARYKTSRLLIALFFAFSIIANNVHYAVYQSWMTGINYWLMLKEVTEVGS
                     AGASMLDKLWLPALWGVAEVVLFCSLAKFRHKTHFSADILFAFLMLMIFVRSFDTKQE
                     HGISPKPTYSRIKANYFSFGYFVGRVLPYQLFDLSKIPVFKQPAPSRIGQGSIQNIVL
                     IMGESESAAHLKLFGYGRETSPFLTRLSQADFKPIVKQSYSAGFMTAVSLPSFFNVIP
                     HANGLEQISGGDTNMFRLAKEQGYETYFYSAQAENQMAILNLIGKKWIDHLIQPTQLG
                     YGNGDNMPDEKLLPLFDKINLQQGRHFIVLHQRGSHAPYGALLQPQDKVFGEADIVDK
                     YDNTIHKTDQMIQTVFEQLQKQPDGNWLFAYTSDHGQYVRQDIYNQGTVQPDSYIVPL
                     VLYSPNKAVQQAANQAFAPCEIAFHQQLSTFLIHTLGYDMPVSGCREGSVTGNLITGD
                     AGSLSIRDGKAEYVYPQ"
     gene            27613..31077
                     /locus_tag="WGQ68_000517"
     CDS             27613..31077
                     /locus_tag="WGQ68_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase"
                     /protein_id="HEZ0714378.1"
                     /translation="MQNMKNQIRGSGMDARSNPANVSDGLQNSSGHIGTNTRYRLTKL
                     GEQMARLPIDPKIARILLAAKKHDCMAEILVIASALSIQDPRERPLEARDAAAKAHER
                     FTDKQSDFLAYLNIWDSFQRERDKGLSNKQLVQWCRQYFLSHLRMREWRELHHQLAQT
                     AIEMGLTTKEAAFRRLSEIKQLTSSENQGDQDLSAKRKQKQLDKKQHRAQIRAAKEAG
                     YEQIHRALLTGLIANVGMKSPDGNDYTGARGSRFHLFPASALFKAKPKWVMAAELVET
                     TKLYARDVAVIQPEWIEQEAPHLVRYHYFEPHWEQKRGEVVASERVTLYGLTVLPRRP
                     VSYGKVAPEEAREIFIRSALVAQECDLKADFFVHNKKLIKEITELEHKSRKQDVLVDD
                     EALFAFYNERLPEMAWKDAQGSVWGSEDSVRIIESDKAERSSENERNEFRKNKRNGSR
                     QNENHGNTVGWVENPTSAATAKTVGFDNPTYATQQPTPSPEREGRGEGKTVAAQTNFS
                     ATAANPLPQEREQSASASTFSDDLRPANLQQTAPSPVGEGWGEGKTVATQTNFSATST
                     NPLPQEREQSASASTFSDDLRPANLQQPSPSPVGEGWGEGKTVATQTNFSATTANPLP
                     NPLPQEGEQSAAASAVSNDPQPKKQPAPQKNRLKPLPLADIRTFQAWLKTAERDNPRL
                     LFLSRDDLMQHAAAHITEEQFPKFWQTADGKFKLSYRFEPHHPLDGVTMTVPLTVLNR
                     LHAPSLEWLVPGMIREKIQLQIKALPKQIRRICVPVPEFITQFLSQNPDRNAPILPQL
                     AQAIAKTAGDIRIFEQINQDEWAAFRLPEHCYFNLRIIDDGGQELAGGRKLHELQQQL
                     GQAAAVTFRDNTQEFERDNVTAWDIGTLPESIKFARGKQQLTGYLGLQKEKDGRIALR
                     LFDTTEAAEQAHRQGVIELMKLQLKEQVKDLNKGIQGFTQAAMLLKHINADTLRDDLT
                     QAVCDRAFIGEDELPRNEKAFKEQIKRARSRLPAVKEALSRYLQETAAVYAELNSKLG
                     KHPLTHLLRLRLQTLLAAGFATRTPWAQWPRLPIYLKAMTLRLEKYSSNPARDAAREA
                     DIQELEQMWQEKTDSLIKQGLPISDGLAAFKWMIEELRVSLFAQELKTPYPVSVKRLL
                     KEWEKIEK"
     gene            complement(31110..32201)
                     /locus_tag="WGQ68_000518"
     CDS             complement(31110..32201)
                     /locus_tag="WGQ68_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEZ0714379.1"
                     /translation="MQTFNRERLEFALDKRGCTQTKLAELLGITARQVRNYVKDEQIP
                     DLNELANLLEFPVHFFTNDERLPELKSQAVSFRARSRTSKRLEKQALNHSITAFLLND
                     WLETEFTLSQAVLPDFSDISPEEAAQELRLEWGLGNEPIPNMITLLEAKGVRVFSLSL
                     DTKDIDAFCTWYENHPFVFLNTQKSAERSRFDAAHELGHLIRDKYSMEHSKNSEITED
                     EPRDIIEKEANAFASAFLMPEAALRLYRHVPITIENLLKIKRRFGVSLVALAYRMHKL
                     GMITDRMYIHNLCPLFAKKKYRTIEPEPMEREISSALRKMLTILKNDGIGIEQIAENL
                     NVSPKDVADLTFGLVERDLNRYRKLRLVK"
     gene            complement(32217..32861)
                     /locus_tag="WGQ68_000519"
     CDS             complement(32217..32861)
                     /locus_tag="WGQ68_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714380.1"
                     /translation="MVEFALSEPFPFEVGEIVEQQNSDIALSEISKYLSQEMLKEIVL
                     TAHNNIQDKAALSDRNTLGMYRQAEITFALRVYLSKDGWTLEPWSGGAFLCLSPCRTK
                     SIIVATATSDVGRHHGMPMTTARKGKGFEEAIRNSKLDLPDTVQFWVLLFHWKYNKVQ
                     LELSLPSGFVSHQITGYKQRIILADVDLTKFDELERKQPVEEYEPLVERKQKAG"
     gene            33218..33430
                     /locus_tag="WGQ68_000520"
     CDS             33218..33430
                     /locus_tag="WGQ68_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714381.1"
                     /translation="MSGIYLPRLFPPHIAERGLLYFQQGKVLDVRKTSAGHYRAEVCG
                     SENYWVWLKLDSDLYIKDESCDCPYV"
     gene            33727..33882
                     /locus_tag="WGQ68_000521"
     CDS             33727..33882
                     /locus_tag="WGQ68_000521"
                     /inference="COORDINATES: protein motif:HMM:NF017953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
                     /protein_id="HEZ0714382.1"
                     /translation="MSERKTKPSPKKGVVFPFIKKLLSINEINPNDPTIGFILLSRNN
                     PDTDYES"
     gene            complement(33972..34421)
                     /locus_tag="WGQ68_000522"
     CDS             complement(33972..34421)
                     /locus_tag="WGQ68_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HEZ0714383.1"
                     /translation="MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRA
                     ALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEGFCIRLNGIARGALDSKFMLKAV
                     AIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK"
BASE COUNT         8696 a        10327 c         8212 g         7285 t
ORIGIN      
        1 tatcatttat cctttctaaa gccgttccgg tttgtccgac cggcggcttt gccccaatat
       61 ccccattttg gagacaccta tgttacgttt gactgcttta gccgtatgca ccgccctcgc
      121 tttgggcgcg tgttcgccgc aaaattccga ctctgcccca caagccaaag aacaggcggt
      181 ttccgccgca caatccgaaa gtgcgtccgt taccgtcaaa accgcgcgcg gcgatgttca
      241 aataccgcaa aaccccgaac gtatcgccgt ttacgatttg ggtatgctcg acaccttgag
      301 caaactgggc gtgaaaaccg gtttgtccgt cgataaaaac cgcctgccgt atttagagga
      361 atatttcaaa acgacaaaac ctgccggaac tttgttcgag ccggattacg aaacgctcaa
      421 tgcctacaag ccgcagctca tcatcatcgg cagccgcgcc gccaaagcat ttgacaaatt
      481 gaacgaaatc gcgccgacca tcgaaatgac cgccgatacc gccaacctca aagaaagtgc
      541 caaagagcgc atcgacgcgc tggcgcaaat cttcggcaaa caggcggaag ccgacaagct
      601 gaaggcggaa atcgatgctt cgtttgaagc ggcgaaaact gccgcacaag gcaaaggcaa
      661 aggtttggtg attttggtca acggcggcaa gatgtccgcc ttcggcccgt cttcacgact
      721 gggcggctgg ctgcacaaag acatcggcgt tcccgctgtc gatgaatcaa ttaaagaagg
      781 cagccacggt cagcctatca gctttgaata cctgaaagag aaaaatcccg actggctgtt
      841 tgtccttgac cgcagcgcgg ccatcggcga agagggtcag gcggcgaaag acgtgttgaa
      901 taatccgctg gttgccgaaa caaccgcctg gaaaaaagga caagtcgttt acctcgttcc
      961 cgaaacttat ctggcagccg gcggcgcgca agagctgctg aacgcgagca aacaggttgc
     1021 cgacgctttt aacgcggcaa aataatgaaa cggcggcatc caatgccgtc tgaaacacgg
     1081 atgcaaaccg cttcctgtgt ttcagacggc attgcccgat acggaggctt caaacaaggc
     1141 tttccgctcc gacggttcgg actgccttgt ttgaatcttc tacgccttaa cgcttttccc
     1201 ttctgtttat gactgccaaa cctttttccc tcaacctgac caacctcctg ctgctggcgg
     1261 tgttgtttgc cgtcagcctg tcggtgggcg ttgccgattt ccgctggtcg gatgtgtttt
     1321 cgctgtccga cagccagcag gttatgttca tcagccgcct gccgcgcacg tttgcgattg
     1381 tgctgacggg cgcgtcgatg gcggtggcgg ggatgattat gcagattttg atgcgcaacc
     1441 gttttgtcga gccttctatg gcgggcgcgg gtcagagtgc ggctttgggt ttgcttctga
     1501 tgtccctgct gctgcctgcc gcaccgctgc cggtcaaaat gtcggttgcc gccgttgccg
     1561 cgttaatcgg gatgttggtg tttatgctgc tgatacgccg cctgccgccg acggcgcaac
     1621 tgatggtgcc tttggtcggg attatttttg gcggcgtggt tgaggcagtg gcgacgttta
     1681 tcgcgtatga aaacgaaatg ctgcaaatgc tcggtgtgtg gcagcagggc gatttttcga
     1741 gcgtgctgct ggggcggtac gagctgcttt ggattacagg cggtttggcg gtgtttgcct
     1801 atctgattgc cgaccggcta acgattttgg ggctgggtga gacggtcagc gtgaatttgg
     1861 gtttgaaccg gacggcggtg ttgtggtcgg gtttgattat tgtggctttg attacgtcgc
     1921 tggttatcgt tacggtcggc aatattccgt ttgtcgggct ggtcgtgccg aacatcatca
     1981 gccgcctgat aggcgacagg ctgcgccaaa gcctgcctgc ggtggctttg ttgggcgcgt
     2041 ctttggtttt attgtgcgac attatcggac gcgtgattgt gtttccgttt gaaattccgg
     2101 tatcgaccgt cttcggcgta ttgggtacgg cgttgttttt atggattttg ttaaggaaac
     2161 ccgcctatgc cgtctgaaaa caaaattgaa tttatgcagg gttcgccgcg cccgttatgg
     2221 gcggcattcg cacttttact gatctgctgc ggactgtttt taacgctcaa tgtcaaaggc
     2281 gattgggatt ttgtactgca cctgcggctg accaaacttg ccgcgctgct gatggtcgcc
     2341 tatgcggtcg gcgtgtccac gcaactcttc caaacgctga ccaacaatcc gattctgacc
     2401 ccttcgattt tgggtttcga ttcgctgtat gtgtttttgc agaccttgct ggtgtttacg
     2461 ttcggcggcg tgggctatac atccctgccg ttgacgggca aattcggctt tgaactggtc
     2521 gtcatgatgg gcggctcgct gctgctgttt tacacgctca tccgtcaggg cgggcgcgat
     2581 ttgccgcgta tgattttaat cggcgtgatt ttcgggattt tgttccgcag cctgtcgtcg
     2641 ctgctttcgc gcatgataga ccccgaagaa tttacggcgg cgcaggcgaa tatgtttgcc
     2701 ggattcaata ccgtccacag cgagcttttg ggcataggcg cgctgatcct gctcgtcagc
     2761 gcggcggtcg tttggcgcga acgctaccgc ttggacgtac accttttggg gcgcgaccaa
     2821 gccgtcaatt tgggcatcag ctacacgcgc aacaccttat ggatactgct ttggattgcc
     2881 gcattggtgg cgacggcgac cgccgtggtc ggccccgtaa gctttttcgg gcttctcgcc
     2941 gcctcgcttg ccaaccactt ttccccgtcc gtgcgccatt ccgtccgcct gacgatgacg
     3001 gtttgtgtcg gcggcatcct cttggtcggc ggacagaccg tattcgaaca cttcttgggc
     3061 atgaaggcgg tattaagcgt ggtggtcgaa tttgcgggcg gactcgtttt cctctatctc
     3121 gttttaaaac acaaaaaatg acggatgccg tctgaacggc agcccgctcc gaaaggacaa
     3181 accatatgac gcaagaacgt ttcccatcat tcttcaacca agccccgacc atcaccgtca
     3241 gagacccgct tgccgcattc ctcggcgcgg cgcaagacgg catcctcact taccgctacg
     3301 ccgatgccgt gcgcctgtgc ggacattcct gcccgaccgt cgcgggcgcg tacctgatgg
     3361 ttatcaaagg tctgaaaaca ctttacggcg aagagctgcc cgaacgcggc ggcatcgaag
     3421 ccgccatgca gggagcgcgc gacgagggca cgaccggcgt aaccgcgtcc gtcgtccaac
     3481 tcctcaccgg cgcagccccc gaaaccggct tcggaggcat cggaatacag ggacgcttcg
     3541 cccgccgcaa cctcttatcc tttggtgcag gcgaaatcaa cggcacactg accctgcgcc
     3601 gcaaagacaa cggcaaaacc gtcgccgtca gcatcaacgc cgccctgaaa cccttcgcac
     3661 ccgaaatgcg cgaacttatg cccaaggccg tcggcggcag cgcaagcaca gaagaactcg
     3721 aacgcttcgg gcaactctgg caggcacgcg ttaaagcatt tttgaccgaa tcggcggacg
     3781 acccgcagtt cgtcatcgtc cgcgaagtgt gagcgttcag acggcattcc gaatttcaaa
     3841 tgccgtctga acccccgcca aacatacaac aacaaaccta cgcccgacaa gcatccgcca
     3901 tgattaccat ccgcaacgtc agctaccgca tcggcacacg ccccatcctc gacaacatca
     3961 gcctcgacat ccccgaaggc ggcattaccg cgctaatcgg cccgaacggc gcaggcaaat
     4021 ccaccctcct ctcctttatg gcgcggcttc agcctctggt acacggcaac atcagctacg
     4081 caggcaaaga catcaaaacc acccctaccg ccgaactcgc ccgcacgctc tccatcctca
     4141 cccaagaaaa cagcatcatg agccgcatta ccgtgcgcga cctgctgatg ttcggccgtt
     4201 acccctacca ccaaggcagg ccgtctgaaa cggataaaac cattgtcgaa gaagcactcg
     4261 ccgagttcca cctgcaagac ttcgccgacc gctacctgac cgagctttcc ggcggccaac
     4321 gccaacgcgc catgattgcc atggtattct gccagcgcac cgactacgtt ttgctggacg
     4381 aaccgctgaa caacctcgac atgtatcatg cccgctccct catgcaaatc ctgcgccggc
     4441 tgaccgacga acacaaacgc accaccgtcg ccgtattgca cgacatcaac caggctgccg
     4501 cttacgccga ccacgtcgtc gccatgaaaa acggcaaagt cgccctgacc ggcacacccg
     4561 aagaagtgtt caccgtacac aatatcaaag aactgttcga catggatgtg tccgtcctcg
     4621 accaccaagg caaaaaactc atcgtccacc atatctgaag ctcaaaaaga aaatgccgtc
     4681 tgaaaacctt tcagacggca tttccattcc aaacgctcaa accgccgcgt ccgcacgttc
     4741 gcccgtgcgg atacggattg cctcctcgac cggcagcaca aaaatcttgc cgtcgccgat
     4801 tttgcccgaa cgcgccacct cgacaatcac gtcaatcgcg cgttccacag cgtcatccgc
     4861 caacaccagc tcaattttga ccttgggcag gaaatcgacg gcgtattccg cgccccggta
     4921 aatctccgta tgccccttct gcctgccgaa ccctttgacc tcgctgacgg tcatccccgt
     4981 aatgccgatt tccgtcaacg cctcgcgcac gtcgtcgagt ttgaacggtt tgacaatcgc
     5041 ctcgattttt ttcatcacat tctccattaa ttaacgtaaa cacacgacaa atatagcaga
     5101 aaccgccggc aaacgataaa cggtactgtt cgcccggcga tgcggcgatt tgccggaaac
     5161 cgtcattccc gcgcaggcgg gaatcccgat ctctgcattt cagcaatatt taaaggtttc
     5221 tgtaaatcca aacttccata ttcctaaccg tgcggaaacg acggcatttg atctaaaaac
     5281 atacagaagc agttgcccgc ccctctactt cctgcaatta tccgcatacc cggctttggg
     5341 taaaaatcag aaaaacagat aaaaccctta ttccttaata tcttcagacg gcacctgtat
     5401 tttccccaac tgtatgccgt ctgaagattg tcaacatttt aagccaaaat atccaagcaa
     5461 tacaaacctg tccggcgtat aatccctctt tttcccaacc gcatttgtga gccgaattat
     5521 gtctgtcgtt ttgcccttgc gcggcgttac cgccctttcc gatttccgtg tcgaaaaact
     5581 cttgcaaaaa gccgccgcac tcggtctgcc cgaagtcaaa ttaagcagcg aattttggta
     5641 tttcgtcggc agcgagaaaa cactggatgc cgcaactgtc gaaaaactgc aagccttgtt
     5701 ggcggcgcaa agcgttgaac aaacgccaaa agcgcgcgag ggcttgcatt tgtttttggt
     5761 cacgccccgt ttgggtacga tttcgccgtg ggcttccaag gcgaccaata tcgcggaaaa
     5821 ctgcggtttg gcaggcatcg agcgcatcga gcgcggcatg gcggtgtggc tggaaggtgc
     5881 gcttaccgat gaacagaaac agcaatgggc ggctttgctg cacgaccgca tgaccgaatc
     5941 cgtattgccc gattttcaga cggcatccaa attattccac catctcgaat ccgaaacctt
     6001 ttccaccgtt gatgttttag gcggcggtaa agaggctttg gtcaaagcca ataccgaaat
     6061 gggtttggca ctctccgccg acgaaatcga ttatctggtt gaaaactatc aggctttgca
     6121 gcgcaatccg tccgatgttg aattgatgat gttcgcgcag gcaaacagcg aacactgccg
     6181 ccacaaaatc ttcaacgccg atttcatcct caacggcgaa aaacaaccga aatccctgtt
     6241 cggcatgata cgcgacacgc acaacgcgca tcccgaaggt acggtcgtcg cttataaaga
     6301 caattcgtcc gtaatcgaag gcgcgaaaat cgagcgtttc tatccggatg cggcggaaaa
     6361 ccaaggctac cgtttccacg aggaagacac gcatatcatc atgaaagtgg aaacgcacaa
     6421 ccacccgacc gccatcgcgc cgtttgcggg cgcggcgacg ggcgcgggcg gcgaaatccg
     6481 cgacgaaggt gcgacgggca aaggttcgcg tccgaaagcg ggcttgaccg gctttaccgt
     6541 gtccaacctc aacatccccg gcctcaaaca gccgtgggaa caagattacg gcaagccgga
     6601 acatatttcc tcgccgctgg acattatgat tgaaggcccc atcggcggcg cggcgttcaa
     6661 caacgaattc ggccgcccca acctcttggg ctacttccgc acctttgaag aaaaatttga
     6721 cggtcaggtt cgcggctacc acaaaccgat tatgattgcc ggcggtctgg gcagcattca
     6781 ggcgcagcag acgcataaag acgaaatccc cgaaggcgca ttgctgattc aactgggcgg
     6841 cccgggcatg cttatcggct tgggcggcgg cgcggcttct tcgatggata ccggcacgaa
     6901 cgacgcgtct ttagacttca attccgtaca acgcggcaac cccgaaatcg aacgccgcgc
     6961 gcaggaagtc atcgaccgct gctggcagct cggcggcaaa aacccgatta tctccatcca
     7021 cgacgtgggc gcgggcggtt tgtccaacgc cttccccgaa ctcgtcaacg atgccggacg
     7081 cggcgcggta ttcaagctgc gcgaagtgcc gctggaagaa cacggtttaa cgcctttgca
     7141 aatctggtgt aacgaatcgc aagagcgtta tgtgttgtcg attttggaaa aagatttgga
     7201 taccttccgc gccatctgcg aacgcgaacg ctgcccgttt gccgtagtcg gcacggcgac
     7261 cgacgacggt catttgaaag tgcgcgacga tttgttcgcc aacaacccca tcgatttgcc
     7321 gctgaacgtc ttgctcggca aaccgcccaa aaccacgcgc agcgacaaaa cggttacgcc
     7381 atctaaaaaa ccgtttcacg ccgacgatat cgacatcacc gaagccgcct accgcgtttt
     7441 gcgcctgcct gccgtagccg ccaaaaactt cctgattacc atcggcgacc gcagcgtcgg
     7501 cggcatgacc caccgcgacc aaatggtcgg caaataccaa acccccgtag ccgactgcgc
     7561 cgttaccatg atgggcttca atacctatcg cggcgaagca atgtctatgg gcgaaaaacc
     7621 gaccgttgcc ctgtttgacg cgcccgcctc gggcagaatg tgcgtcggcg aagccatcac
     7681 caacatcgcg gcggtcaaca tcggcgacat cggcaatatc aaactctccg ccaactggat
     7741 ggcggcgtgc ggcaacgaag gcgaagacga aaaactctac cgcaccgtcg aagcggtttc
     7801 caaagcctgt caggcattgg atttgagcat tcccgtgggc aaagacagcc tgtcgatgaa
     7861 aaccgtatgg caggatggcg aagagaaaaa atccgtcgtt tcgccgttga gcctgattat
     7921 ctccgcgttc gcgccggttc aagacgtacg caaaaccgtt acgcccgaat tgaaaaacgt
     7981 cgaggacagc gtattgctgt ttgtcgattt gggcttcggc aaagcgcgta tgggcggctc
     8041 tgcattcggt caggtataca acaacatgag cggcgacgcg cccgatttgg acgacacagg
     8101 ccgtctgaaa gcgttttaca acgtgattca acagcttgtc gccgaagaca aactcttggc
     8161 ataccacgac cgcagcgacg gcggcttgtt tgccgttttg gtagaaatgg cgtttgcggg
     8221 gcggtgcggc ttggatatag atttaaattt attgcttgca caaacattta ttaccaacca
     8281 taccgctctg tctcaatcat tgcggactga agaggtaaaa gcgttggctg aatggcaaga
     8341 aaccattgcc cgcacattat ttaatgaaga gttgggtgct gttatccaag ttagaaaaca
     8401 agatgttgcc gatattatca atttattcta tcaacaacag ctgcatcata atgtctttga
     8461 aatcggtacg ttaactgatg agaacacgtt aatcatccgc gacgggcaaa cgcaccttat
     8521 ttctgacaac ctaatcaaac tgcaacaaac ctggcaagaa accagccatc aaatccaacg
     8581 cctgcgcgac aaccctgcct gcgcagacag cgagttcgcc ctgattggcg acaacgaccg
     8641 cagcgcattg tttgccgacg tgaaatttga cgtgaacgaa gacatcgccg cgcctttcgt
     8701 caacagcggc gcgaaaccca aaatcgccat cctgcgcgaa cagggcgtaa acggacaaat
     8761 cgaaatggcc gccgccttca cccgcgccgg tttcgatgcc tacgacgtgc atatgtccga
     8821 cctgatggcg ggccgcgtca accttgccga cttcaaaatg ctggcggcgt gcggcggctt
     8881 cagctacggc gacgtacttg gtgcgggcga aggctgggca aaatcgattc tgttccaccc
     8941 tgctctgcgc gaccaatttg ccgctttctt tgccgatccg aacacgctga cattgggcgt
     9001 gtgcaacggc tgtcaaatgg tcagcaacct tgccgagatt atcccgggcg cagaagcatg
     9061 gcctaaattc aaacgcaact tgagcgaaca gtttgaagcg cgcctaagca tggttcacgt
     9121 cccaaaatca gcctccctga ttctgaacga aatgcaaggc tccagcctgc ccgtcgtcgt
     9181 cagccacggc gaaggccgcg ccgacttcgc gcttcacggc ggcaaaattt cagacgacct
     9241 cggcatcgcg ctgcaatacg tcgacggaca aaaccaagtt acccaaacct acccgctcaa
     9301 ccccaacggc tcgccgcagg gcatcgccgg cgttaccaac gccgacggcc gcgttaccat
     9361 catgatgccc caccccgaac gcgtgtaccg cgctgcacaa atgagctgga aaccggaaga
     9421 ctggacggaa ctctccggct ggtaccgcct ctttgccggc gcacgtaaag ccttgggcta
     9481 accgccctac tcaaaccaat gccgtctgaa gaatatttca gacggcgttc cggcatacca
     9541 tcctttaaac ggtatccgtc caccgaggaa cactcatgaa aatcacaccc gtcaaagccc
     9601 taaccgacaa ctacatctgg atgatacagc acggcaacca tgccgtctgc gtcgaccctt
     9661 ccgaaccctc gcccgtcttg gaattcctcg tccgcaaccg cctcatgctt gcccaaacat
     9721 gggtaacgca cccccacccc gaccacgagg gcggtgcggc ggcactctgg cgcggctaca
     9781 tggaatcgcc cgtttacggc gaatccgaca tcgaagccgc cacccacacc gtaaccgccg
     9841 gcacccaatt caccttcggc gacggacagg ttaccgtttg ggcaacaccc ggccacacag
     9901 accgccacac cagctacctt ctcgaaactt cagacggcat acacgtcttt tgcggcgaca
     9961 cccttttttc cgccggctgc ggacgcgtgt ttaccggcac aatcgaacag ctttacgaca
    10021 gcttccaacg cttcaaccgc ctgcctgaaa acaccctgtt ctatccggcg cacgaataca
    10081 ccgccgccaa cctggatttt gccgcccata tcgagccgga caacgccgac attcagacgg
    10141 cactgaaagc ggcggcgcat acgcctaccc tgcccgttac cctcgcgcac gaacgccgtg
    10201 tcaatccgtt tttgcgcgtc gacctgccgc acgtcagaga ccgcgccgag gcattgagcg
    10261 ggaaaacgtt aaacagcagc ctcgatacct ttgtcgcgct gcgtgaactt aaaaaccaat
    10321 accggacgaa ataaaacaac gggaaaacgc agccattcct aggattttta ttaaaatctt
    10381 aaataaaatc atacaatcat cgccaataga cgaaaggaca ccgttgcctt ataatcaaac
    10441 aaaaacaaaa tatataatat agtggattga atttaaatca ggacaaggcg acgaagccgc
    10501 agacaataca gatagtacgg caaggcgagg caacgctgta ctggtttttg ttaatccact
    10561 atattgttaa tccactatat aaatccagca caaaacggga tcggtgattc ttgtccgcaa
    10621 gaatcgttga ttttctctat tacacggata atcatcatgc gcttcacaca caccacccca
    10681 ttttgttccg tattgtccac cctcggtctt tttgccgttt cccctgctta ctcatccatt
    10741 gtccgcaacg atgtcgatta ccaatatttt cgcgactttg ccgaaaataa aggcgcgttc
    10801 gccgtaggtg caagcaatat ttccatccaa gacaagcaag gcaaaatatt aggcagggtt
    10861 ctcaacggca tccccatgcc cgacttccga gtcagcaacc ggcaaaccgc catcgccacc
    10921 ctgattcacc cccaatacgt caacagtgtc aaacacaacg tcggctacgg ttccatacaa
    10981 ttcggcaacg acacccaaaa tccagaagaa caagcctata cctaccgcct cgtatcacgc
    11041 aacccgcacc cggactacga ctaccacctt ccccgcctca acaaactggt taccgaaatc
    11101 tcacctaccg cactcagcag cgtacccttg cttggaaacg gccagccaaa ggccaatgcc
    11161 tacctcgata ccgaccgctt cccctacttt gtacgactcg gctcaggcac gcaacaagtc
    11221 cgcaaagcag acggcacgcg tacacgaacc gccccggcat accaatacct gaccggcggc
    11281 acgccgctga aagtattggg gttccaaaac cacggcttac tcgtcggcgg cagcctgacc
    11341 gaccaacccc ttaacaccta cgcaatcgcc ggagacagcg gttcccccct gtttgccttc
    11401 gacaagcatg aaaaccgctg ggtgcttgcg ggcgtactca gcacctacgc cggcttcgat
    11461 aacttcttca acaaatacat cgtcacgcaa cccgaattca tccgttccac catccgccaa
    11521 tacgaaaccc ggctggatgt cgggctgacc accaacgaac tcatatggcg cgacaacggt
    11581 aatggcgaca gcaccctgca agggctcaac gaacgcatca ccctgcccat tgcaaaccct
    11641 tcgcttgccc cacaaaacga cagcaggcac atgccgtctg aagatgccgg caaaacgctc
    11701 atcctatcca gcaggttcga caacaaaaca ctgatgctgg cagacaatat caaccaaggc
    11761 gcaggcgcat tgcagttcga cagcaacttc accgtcgtcg gtaaaaacca cacatggcaa
    11821 ggtgcaggcg ttatcgtagc cgacggcaaa cgcgtcttct ggcaagtcag caaccccaaa
    11881 ggcgaccggc tctccaaact gggcgcaggc acgcttatcg ccaacggaca aggcatcaac
    11941 cagggcgaca tcagcatcgg ggaaggcact gtcgtactcg cccaaaaagc tgcttcagac
    12001 ggcagcaaac aagcattcaa ccaagtcggc atcaccagcg gcaggggcac ggccgtcctc
    12061 gccgacagcc agcaaatcaa acccgaaaac ctctatttcg gcttcagggg cggacggctc
    12121 gacctcaacg gcaacaacct tgcctttacc catatccgcc atgcggacgg cggcgcgcaa
    12181 atcgtcaatc acaaccctga ccaagccgcg acactgacgc tgaccggcaa ccccgtcctc
    12241 agtcccgagc atgtcgagtg ggtgaaatgg ggcaaccgtc cacaaggcaa cgcggcggtt
    12301 tacgaataca tcaacacgca ccgcaaccgt cggaccgact acttcatact caaacccggc
    12361 ggcaacccgc gcgaattttt cccgttaaat atggaaaact caacaagctg gcaatttgtc
    12421 ggcaacaaca ggcaacaggc cgccgaacaa gtcgcccaag ccgaaaatgc ccgccccgac
    12481 ctgattacct tcggcggata cttgggtgag aacgcgcaaa cgggtaaagc cgcaccgagt
    12541 tacagcaaaa ccaatatcga atgggtgaat agtccaaaca ccccacacaa gccctcgccc
    12601 tatacgaata cgccaacccg cacaggggca ggcgtaccga cgattaccgc ctcaaagaag
    12661 gcggcaaccc cggcgcctac ttcccatggg atgcctacag cacccaagac tgggaatttc
    12721 tcggacataa ccggcgaagc agccatagaa aaaacccgcc atatcgcaaa tgccgccgta
    12781 tacggccggc ccgaataccg ttacaacggc gcactcaacc tgcactatcg tcccaaacgc
    12841 accgacagca cgctgttgct caacggcggc atgaacctta acggggaagt cttgattgag
    12901 ggcggcaata tgattgtgtc aggcaggccc gtaccccatg cctacgacca ccaggccaaa
    12961 cgcgaacccg ttcttgaaaa cgaatggacc gacggcagct tcaaggctgc acggttcacc
    13021 ctgcgaaacc atgcccgact gacggcaggg cgcaataccg cgcatctgga cggcgacata
    13081 accgcatacg atctgtccgg catcgacctc ggctttatcc aaggcaaaac accggaatgc
    13141 taccgctcct accatagcgg cagcacccac tgcacaccca acgccgtttt aaaagccgaa
    13201 aactatcgtg cactacctgc aacgcaagta cgcggcgaca ttacccttaa cgaccgttca
    13261 gagctccgcc tgggcaaagc acacctgtac ggcagcatcc gtgccggcaa agacaccgca
    13321 gtccgcatgg aagcagacag caactggaca ctttcccagt ccagccacac cggcgcactg
    13381 acgcttgacg gcgcacaaat taccctgaac cccgatttcg ccaataatac acacaacaac
    13441 cgcttcaaca cactgaccgt caacggcaca cttgacgggt tcggcacatt ccgattcctg
    13501 accggcatcg tccgaaaaca aaatgccccc ccctcaaact ggaaggggac agccgcggcg
    13561 cattccaaat ccacgtcaaa aacaccggac aagaacctca aacaaccgaa tcgcttgcac
    13621 ttgtgagcct caatccgaaa cacagccacc aagcccgatt caccctccaa aacggctatg
    13681 ccgatttggg tgcctaccgc tacatcctcc gcaaaaacaa caacggatac agcctgtaca
    13741 acccgctcaa agaggccgaa cttcaaattg aagccacgcg tgcggaacat gagcgcaacc
    13801 aacaggcata caaccaatta caggcaaccg acatcagcag acaggttcaa catgactctg
    13861 acgcgaccag gcaggcacta caggcctggc agaacagtca aaccgaactt gcccgcatcg
    13921 acagccaagt ccaatatctg tccgcccaat tgaaacagac agacccgcta accggcattc
    13981 tgacgcgtgc ccaaaacctg tgtgccgcac aaggatacag tgtcgatatc tgccgtcagg
    14041 ttgccaaagc cgccgacacg aacgacctga cactcttcga aaccgaactg gatacgtata
    14101 tagaacgtgt agaaatggcc gaatccgaac ttgacaaagc acggcaaggc ggcgatgcgc
    14161 aagccgtcga aacagcccgg cacgcctacc tgaacgcact caaccgtctg tcccgacaaa
    14221 tccacagttt gaaaaccggc gttgccggca tccgtatgcc gaacctggcc gaactgatca
    14281 gccggtcggc caacaccgcc gtttccgaac aggccgccta caataccggc cggcaacagg
    14341 cgggacgccg catcgaccgc caccttaccg atccgcagca gcaaaacatc tggctggaaa
    14401 ccggtacgca acaaaccgac taccatagcg gcacacaccg tccctaccaa caaactacca
    14461 actatgcaca tatcggcatc caaaccggca tcaccgaccg tctcagtgtc ggtacgattt
    14521 taaccgatga gcgcacaaac aaccgttttg atgaaggcgt atccgcccga aaccgcagca
    14581 acggcgcaca tctgttcgtc aaaggggaaa acggcgcact ctttgccgcg gcagatttag
    14641 gctacagcaa cagccgtacc cgatttaccg attatgacgg ggctgccgtc cgccgccacg
    14701 catgggatgc aggcatcaac accggcatca aaatcgatac cggcatcaac ctcagaccct
    14761 atgccggcat ccgtataaac cgcagcaacg gcaaccggta cgtactcgac ggcgcagaga
    14821 taaacagccc ggcgcaaatc caaaccacat ggcatgccgg catccgtctc gataaaaccg
    14881 tcgaactggg tcaagccaag ctgacccccg ctttcagcag cgattactac catacccgcc
    14941 aaaacagcgg ttccgccctc agcgtcaacg accgtacctt actgcagcaa gccgcccacg
    15001 gcacactgca taccctgcaa atcgacgccg gatacaaagg ctggaacgcc aaacttcatg
    15061 ccgcttacgg caaagacagc aacaccgccc gccacaaaca ggcaggaatc aaaataggct
    15121 acaactggta acaagccgat aaaaatgccg tctgaaaccc gcgtttcaga cggcatttgc
    15181 gttaaaaata gtaaaccgtt ccaaaaggga gtagaatagt gccgtttcca accctgcgcc
    15241 tgtaccgtca ggcttttatt atggaccttc ccagttcgct tttactgaac gccccttccg
    15301 attccaaacg acaataacca tcccgccgga atgcctcccc gcacacggcg ggcggagcat
    15361 ttatgagcat cgaacacact cctcccacac acgacggcga aaccggtcaa aaccatgccg
    15421 aacgcccttc cgccgatttc gaccgtgtcc actccctctg cgaaatcctc gaacctgctt
    15481 ttgaacaaat cgaaaacggt acaccgcttg aagacgcgcc gctgcgcgac aagctgaccg
    15541 agctgaccgt cctcttgagc gagctgcacc ctgccgacgt ggcggcggtg ttggaatcgc
    15601 tgccgccgcg cgaacgcaac gtcgtttggc ttctggtcaa acccgaagac gacggcgaag
    15661 tattgctgga agtatccgac gcggtgcgcg aaacactgat cgagtcgatg gataaggacg
    15721 aattgttggc ggcagttgac gatttggacg cggacgaatt ggcggaactg gcagacgatt
    15781 tgccgcacca agtggtttat gaagccttgc agacgcgcga cgaggaagag cgcgcgcaag
    15841 tcaaagcggc aatgtcctac gaagacaacc aagtcggtgc gattatggac ttcgagttgg
    15901 tcagcatccg cgccgatgtc gcctgtgaag tggtgctgcg ttacctgcgc cgcttcgaca
    15961 gcctgcccga ccataccgac aagatttttg tggtcgatga aaacgatgtg ttgcagggcg
    16021 tgctgcccat ccgcaaactt ttggtcgccg atcccgaaga cttggtggaa aacgtgatgg
    16081 cgaaagatgt cgtgcgtttc cgcgccgaag acgacgtgga agaagcggcg caggcgtttg
    16141 aacgctacga cttggttacc gcgcccgtcg tcgatgaaaa caaaaagctc atcggtagga
    16201 ttaccgttga cgagatggtg gacgtcatcc gcgaagaatc cgaagccgat atgttgaaca
    16261 tggcaggttt gcaggaagag gaagacctgt tcgcccccgt atgggattcg gtgaaaaacc
    16321 gctggatgtg gctcgccgtc aacctctgca ccgccttcct cgccagccgc gtcatcggcg
    16381 cgtttgaagg cagcatcgaa aaaatcgtcg cactcgccgc gctgatgccc atcgtcgccg
    16441 gtatcggcgg caactcgggc aaccagacga ttaccatgat tgtccgcgca atggcgatgg
    16501 ggcagttgac gggaatgcag gcggggcgct tgttgaaaaa agaagtcggc gttgccctag
    16561 tcaacggcat tatctgggga accgtcatgg gcgcggtttc atggctgctc tacggcagcc
    16621 tcggcatcgg gctggttatg attgccgcga tgacgctcaa cctcctgctg gcggcaaccg
    16681 tcggcgtatt aattcccgtg gtaatggaaa agttcggacg cgatcccgca ctgggcagct
    16741 cggtgctgat taccgccgtt accgactccg gcggcttcct gattttcttg gggctcgcca
    16801 ccctattcct gctttaaatg ccgtctgaac ccgcgcaaaa atgccgtctg aagcggaagc
    16861 tgcttcagac ggcatttgac tatttatcct tgttgcacaa gattattgga cggtatgccg
    16921 gggcagccct ttggcaacgc cgaccacatc ctccccgaac agcgcgttga catcggtttc
    16981 gtcaaacaca tatttgctgt ggcagaaatc gcaatcgact tcgatgctgc cttgttccac
    17041 caccacgccg ccgacttctt ccccgcccag catcaacagc atatcgctga ctttgccgcg
    17101 cgaacaggtg catgaaaatt caaacgtttc cggctcgaac acgcgcggcg gcgtttcgtg
    17161 gaacaggcgg tataaaacgt gttgcgcgtc cagtcctgcc agctcctccg ccgtcagcgt
    17221 gcgcgccagc gtactgacgt gttcccatgc ctcttcatcc aacaccgctt caggcagacg
    17281 ctgcaccagc agaccgcccg ccgcttcgtc gcttgcagac aggacgatgt gcgtatcgat
    17341 ttgttcggaa cgcttcatat agttcgccaa catttgcgcg ataccgccgc cttccaaagg
    17401 cactacgccc tgccagggtt cgccgtcttt gggctgcagc gtcagcacga atacgccgcc
    17461 ctcgcccaaa aggtcgccga ggctttcgtc atcggctatt tctgcggttt cgtcccaacg
    17521 cgcggttgca cggacggtac ggtcggaagc cgcttccgca accagcattt tcagccgccc
    17581 ccgcccctga acctgcacaa tcagcgtgcc ttcgtttttg aggttgcccg acagcaacac
    17641 acccgccgcc aacaactcac ccaaagcgcg gcggatggcg gcgggatagt ttttctgttt
    17701 tacaatgtgc tgccatacgt tttctagacg gacgtgcagc ccgcgcacgg gcatatcgtc
    17761 gaagataaag cgggtacgca catcggcgcg gttgatggcg gtttgattca tgattttctc
    17821 tgactgattg ttcggatggc ggctatatgg ttgcggtcgg cgcgaaaaca agacggacgg
    17881 cggatgcgct tcccaaatta tcaataaatt atataaaaat caacatatta actcaatcta
    17941 acaagccgtt ttttgccaaa cagccgtttt tttatataca atcaacaaga tattttcgac
    18001 tgatacagca taacatcgca cggcggcacg atgcctcctg cgcggaaaca ccgatatgga
    18061 ttcttttttc aaaccggcag tttgggcggt tttgtggctg atgtttgccg tccgccccgc
    18121 ccttgccgac gagttgacca acctgctcag cagccgcgag cagattctca gacagtttgc
    18181 cgaagacgaa cagcccgttt tacccatcaa ccgagccccc gcccggcggg cgggcaatgc
    18241 cgacgaactc atcggcagcg cgatggggct taacgaacag cccgttttac ccgtcaaccg
    18301 agtccccgcc cggcgggcgg gcaatgccga cgaactcatc ggcaacgcga tggggcttaa
    18361 cgaacagccc gttttacccg tcaaccgagc ccccgctcgg cgggcgggca atgccgacga
    18421 actcatcggc aacgcgatgg gacttttggg tattgcctac cgctacggcg gcacatcggt
    18481 ttctaccggt tttgactgca gcggcttcat gcagcacatc tttaaacgcg ccatgggcat
    18541 caacctgccg cgcacgtcgg cagaacaggc gcggatgggt acgccggttg cccgaagcga
    18601 attgcagccc ggggatatgg tgtttttccg cacgctcggc ggcagccgca tttcccatgt
    18661 cggactttat atcggcaaca accgcttcat ccacgcgccg cgcacgggga aaaatatcga
    18721 aatcaccagc ctgagccaca aatattggag cggcaaatac gcgttcgccc gccgggtcaa
    18781 gaaaaacgac ccgtcccgct ttctgaactg atttcccaag gaatacgcaa tgagtatgcc
    18841 cgaaatgccc aaatggtacg acgatgacgg acagatcgtg tcctgtaccg aaaaggtcaa
    18901 agtgatgtcc gaaaatatgg ccgagctgta tcagacggca caagacgcgt ttgaagacgc
    18961 gctgctgatg ggttgcggcg aacgtcagtt gcgcgattac ctgctcgcgc tgattgaagg
    19021 tttggaaaat ccctaccgca aagtctgaac acgccccggt tgctgcggca cggtttatcc
    19081 gtgccgtttt tgcgtttgtg cgcggcttcg gcttttcaga cggcatattt gacgttatga
    19141 ttaaacagtt aacaagattt atcacaacgc cgtcaaaaga cagacacaca acatgaacat
    19201 catccgcgcg ctcctcatca tcctcggctg cctcgccgcc ggcgaaaccg ccgttttcct
    19261 agcaggcatc aaactgcccg gcagcatcgt cggcatgggc gtgctgtttg cgcttttgca
    19321 ggcgggttgg gtcaaaacgt cttggctgca acagcttacc gacgcgctga tggcgaatct
    19381 gacgctgttt ctcgtgccgc cctgcgtggc ggtcatcagc tatttggatt tgattgccga
    19441 cgattggttt tcgatactgg tttccgcctc cgccagcact ttgtgcgtac tgctggttac
    19501 aggcaaggtt caccgctgga tacggagcat tatctgatga acgaaatcct caggcagccg
    19561 agcatcctgc ttttcctcac gcttgccgtg tacgcgcttg cgattatcgt gcgcacgcgc
    19621 acgggtaata tcttctgcaa ccccgtactc gtcagcacta tcgtgctgat tgcctacctg
    19681 aaaatcctcg gtatcgatta tgcggtgtac cacaacgccg cgcaattcat tgatttttgg
    19741 ctaaaacccg ccgtcgtcgt gcttgccgtg ccgctctacc aaaaccgccg taaaatcttc
    19801 aaccaatggc tgcccgtcat cgtttcacag cttgcgggca gcgttacggg cattgttacg
    19861 gggatgtatt ttgccaaatg gctgggcgcg gaacgcgaag tcgtcctctc gctcgcgtcc
    19921 aaatctgtta ccaaccccat cgccattgaa atcacccgct ccatcggcgg cattcccgcc
    19981 attaccgccg ccaccgtcat cattgccggt ctggtcggac agattgccgg ttacaaaatg
    20041 ctgaagaaca cggtcgttat gccctcatct gtcggaatgt cgctcggcac ggcttcgcac
    20101 gcgatgggca ttgccgcctc gctcgaacgc agccgccgca tggcggcata cgcggggctg
    20161 gggctgacgt tcaacggcgt actgaccgcg ctgattgcgc cgctgcttat ccccgttttg
    20221 ggattttgaa cccgtttcag acggcatttc agcccatgct gtctgaacgc cgacacactc
    20281 gcaaggagaa ccgttatggc tgtcaacctg actgaaaaaa ccgccgaaca actgcccgac
    20341 atcgacggca ttgccctcta caccgcccaa gcaggcgtga agaagcccgg gcataccgac
    20401 ctgacactga ttgccgtagc cgccggcagc accgtcggtg cagtcttcac gaccaaccgt
    20461 ttctgtgccg cgcccgtcca catcgccaaa tcgcaccttt tcgacgaaga cggcgtgcgc
    20521 gccctcatca tcaacacggg caacgccaac gcgggtacgg gcgcacaggg cagaatcgat
    20581 gctttggcag tgtgtgccgc cgccgcccgg caaatcggct gcaaaccgaa ccaagtgctg
    20641 cccttctcca ccggcgtgat tctcgaaccg ctgcccgcag acaaaatcat cgccgcgctg
    20701 cccaaaatgc agcctgcctt ctggaacgaa gcggcacgcg ccatcatgac caccgacacc
    20761 gtgcccaaag ccgcctcgcg cgaaggcaag gtcggcgaca aacacaccgt ccgcgccacg
    20821 ggcatcgcca aaggctcggg catgattcat cccaatatgg cgaccatgct cagtttcatc
    20881 gccaccgatg ccaaagtttc ccaacccgtc ctccaactga tgacgcagga aatcgccgac
    20941 aaaaccttca acaccatcac cgttgacggc gacaccagca ccaacgacag cttcgtcatc
    21001 atcgccaccg gcaaaaacag ccaaagcgaa atcgacaaca tcgccgaccc gcgttacgcc
    21061 caactcaaag aattgttgtg cagcctcgcg ctcgaactcg cccaagccat cgtccgcgac
    21121 ggcgaaggtg cgaccaagtt catcaccgtc cgcgtcgaaa acgccaaaac ccgcgacgaa
    21181 gcccgccaag ccgcctacgc cgtggcacgt tcgccgctgg tcaaaaccgc ctttttcgcc
    21241 tccgacccca acctcggcag gctgctcgcc gccatcggtt atgccggcgt tgccgacctc
    21301 gataccgacc tcgtggaaat gtatctcgac gatattttgg ttgccgaaca cggtggacgc
    21361 gccgcaagct acaccgaagc acaagggcag gcggtgatgt cgaaggccga aatcaccgtc
    21421 cgcatcaagc tgcatcgcgg acaagccgcc gccaccgtct atacctgcga cctgtcgcac
    21481 ggatacgttt ccatcaacgc cgactaccgt tcctgacccg acacggcttc agacggcata
    21541 cataaaatgc cgtctgaacc gccggacaac ataccatgac ctccacattc ccccgccgcc
    21601 tcgcccgcaa aatccgccaa acccgccgcc tgtcgcgcaa aagcatcgcc tttctgttcc
    21661 ttttggcagg ttcggcactc gtcgccctga ccgcgctgtt ttttgcccat cttgccgatt
    21721 ttgcgctgga actgaacgcc aaactggttc aacaataccc gtggttcgcg tgggtcgcgc
    21781 ttcctttggg tttaccgctt attgcgtggc tcacacgcaa attcgccccc ttcaccgccg
    21841 gcagcggcat cccgcaggtc atcgcctcac tgtcgctgcc ctacggcgca cagaaaacgc
    21901 ggctgatccg cctcgggcag acgctgctga agattccgct aacctttttg ggtatgctgt
    21961 tcggcgcgtc catcggacgc gaaggtccgt ccgtgcaggt cggcgcggca gtgatgggcg
    22021 cgtggggcgc gtggtgcaag aaacacggct tggcattcaa agggatgcag gaaaacgatt
    22081 tgatggcggc gggcgcggcg ggcggtttgg cagccgcgtt caacgcgccg ctggcgggcg
    22141 tgattttcgc cattgaggaa ctcgggcgcg gcatcatgtt gcgctgggaa cggcagattc
    22201 tgctcggcgt actcgccgcc ggtttcatac aggtcgccat tcagggcaac aacccgtatt
    22261 tttccggctt caacggcggc gtattggaac atatctttct gtgggtcgca ctgtccggcc
    22321 tggtttgcgg cgcggcgggc gggctgttcg gacgtttgct ctatcgcggt gcggcggcgt
    22381 ttgcaccgcg caagatacgc ggcttcatcc gcaaccgtcc gctgctgctg gcggcactga
    22441 tggggctgct gctcgccctg ctcggcacgt tctaccaagg caaaacctac ggcaccggct
    22501 accacgaagc cgcccaagcc ctgcacggca tctacgaagc ccccttcgga ctcaccgccg
    22561 ccaaatggct cgccaccgta ttcagctatt gggcaggcat tccgggcggc attttcactc
    22621 cctcgctgac cataggcgcg gttttgggcg agcatatcgc cgccatcgcc gacatatcgc
    22681 agggtgcaaa catcatcgtc ctcatctgca tggcggcatt tctggcgggc gcgacacaat
    22741 ccccgattac ttccgccgtc gtcgtcatgg aaatgacggg cggacaaagc ctgctgtttt
    22801 ggatgctgat tgcctgcatt ttcgcctcgc aggtttcgcg ccagttttcg ccgcgtccgt
    22861 tctaccacgc atcgggaatg cgcttccgcc agcgcgtgct tcaagaaacc gccgcccaaa
    22921 ccggcaatgc gcccgcaaga ccgcaaacag caaacagcaa aacgggaatg ccgtctgaaa
    22981 attaaaacgc ccccgatcaa acgccggcag ccgccttgat tcgaataccg ttccgccgcc
    23041 gcttgaaatt tcagcaacaa tgccgtctga acgacagaat gcggttttca gacggcattt
    23101 ccccatcccg atattgccta aacaaaaccg aagcgtttgc tataattgca ctttttaccg
    23161 cacacgcacg cccatgtttc ccgatttctc ccaaaccctc tccaaagacc gccacttcct
    23221 gcaatccgcc ttcaaaaatc ccaacaaata cggcggtttg tccaaagtcg aagaaaaata
    23281 ccgaaaatcg cacgaaatct ttttgaggcg tttggccgca ttgcccaaac ccgaattcga
    23341 caacaccctg cccgttcacg aaaaactcga agaaatcaaa aaaaccgttg ccgaacatca
    23401 ggtaacgatt atttgcggcg aaaccggttc gggcaagacc actcagttgc ccaagatttg
    23461 tttggaactc gggcgtgggg cggcggggct gatcgggcat acccagccgc gccgtttggc
    23521 cgcgcgctcc gtggcagagc ggattgccga agagctgaaa tccgaaatcg gcagcgcggt
    23581 cggctataaa gtacgcttca ccgaccacac ctcgcgcgat gcctgcgtca agctgatgac
    23641 cgacggcatc ctgctggcgg aaacccagac cgaccgttat ctcgccgcct acgacacgat
    23701 tatcatcgac gaagcgcacg agcgcagcct gaacatcgac ttccttttgg gctatttgaa
    23761 acaactcctg ccgcgccgcc ccgatttgaa agtcatcatc acctcggcaa cgatagacgc
    23821 agaacgcttt tcccaacact tcaacggcgc gcccgtttta gaagtgagcg gacggacgta
    23881 tcccgtcgaa atcctctacc gaccgctgac cggcaaagac gaagacgacg cagaagtgga
    23941 gttgaccgac gcgattgtcg atgcggcgga cgaattagcg cgacacggcg aaggcgatat
    24001 tttggtattc ctgccgggcg agcgcgaaat ccgcgaagcc gccgaagccc tgcgcaaatc
    24061 cacgctgcgc cgcaacgacg aaatcctgcc cctgttcgca cgcctgtcgc acgccgagca
    24121 gcacaaaatc ttccacccct caggcgcgaa acgccgcatc gtattggcaa ccaacgtcgc
    24181 cgaaacctcg cttaccgtgc cgggcatcaa atacgtcatc gacaccggcc tcgcgcgcgt
    24241 caaacgctat tccgcacggg caaaggtgga gcagcttcat gtcgaaaaaa tctctcaagc
    24301 cgccgcccgc caacgctccg gccgctgcgg acgcgtctcc gcaggcgtgt gtatcagact
    24361 gttttcagaa gaagatttca acagccgccc cgaatttacc gacccagaaa tcgtccgcag
    24421 caacctcgcc gccgtcatcc tgcgcatggc atcgctgaac ctgggcgatg tggcggcatt
    24481 cccattttta gaaatgcccg attcgaggta tattaatgat gggtttcagg tgttgctgga
    24541 attaggggca gtggaggccg cctgaaaaca agtaggatat acacttctac ctgacaaaga
    24601 ccaacaaacg ataaaactac ggcacatcta gatgcccaac ctacaacatt gcacacggta
    24661 aaaacatgaa acacttactc atcgattttg aaaacgtcca gccgcaaaac ttagacaaat
    24721 tactaaccga aaatacccat atttggctat ttataggtgt attacacaaa atgttaccta
    24781 ttagtctggt gcaatcccta ctacgtttcg gcgaacgtgt ccatcttgtc cagttacaaa
    24841 aaacggggaa aaacgcattg gatttttacc tgtcctatta cctcggacaa attaccgcca
    24901 cagaccccaa tgcccaaatc ggcatactct cgcgtgatgg aggatacgat gttctggtcg
    24961 aacatatttt gaaaaaccac caggcgaagg gtatcgtgcg cctagccaat atagatgaag
    25021 tacaacatca gaaaattact accgaaccgc cgtcagcatt gctggaaaac actcctcagc
    25081 ctgaaaccac cctcaaacca cagcaaccat taacttccta tttccaagca gccctaactg
    25141 cactgcgccg ccccgacgct ttccgcccct gccgcctgca taacctgcga caaaatctgc
    25201 gtaagcatat tttgagtgat ttgtttaaag aaaaaaccga tgaagaatgc gaaataacca
    25261 ctgctaacgt tatcaataaa ctcaaagcac aaaacttcat cagcattgat gaacaggaaa
    25321 ccgtttccta ccatctcagt gataatgatt tgttacaaag aatccaacgc catattttaa
    25381 gccaacgtcc caaaacctac gctgattttc aagccgtcgt gcaaaaccga gcagatgcac
    25441 ttcacttaac agtcggtacc aacgacattc aatcctttgc gcgacatttg cgcgaccaaa
    25501 acctgatccg ccaaaacaat gggaaaattg aatatgcacc gtttactgaa cctaaaccac
    25561 agccaacgcc caagcagcct aaaaaaaccg catgggaacc tgatgaaatt atttggaaaa
    25621 aagtgattgc cgcgttatcg ttaaagaacc gtcctaataa aaccaaaact ttacgcaata
    25681 caatccaggc actcacaaaa tccaatgcac aagaaactga caaactgcta caacatttac
    25741 aagataccca agtcctacgt attgacggta ctaaaattgt gtatatcaag taggatgggc
    25801 atttatgcct gacctacaaa accaaaaaca acctaacacc cttaatcaac tccacaaacc
    25861 cttttcagac aacctcgttt tttgaaaaac aatctgtaaa cagataactg ctgaagaata
    25921 ccgttgccga gccccaaaac ctgcctgcaa cttttattgt gaactgccca ttatgaaaaa
    25981 atcccttttc gttctctttc tctattcatc tctacttacc gccagcgaaa tcgcctatcg
    26041 ctttgtattc ggaattgaaa ccttaccggc tgcaaaaatg gcagaaacgt ttgcgctgac
    26101 atttgtgatt gctgcgctgt atctgtttgc gcgttataaa acttcgcggc tgctgattgc
    26161 gctgtttttc gcgttcagca ttattgccaa caatgtacat tatgcggttt atcaaagttg
    26221 gatgacgggt attaactatt ggctgatgct gaaagaggtt accgaagtcg gcagtgcggg
    26281 cgcgtcgatg ttggataagt tgtggctgcc tgcgttgtgg ggcgtggcgg aagtcgtgct
    26341 gttttgtagc cttgccaagt tccgccataa gacgcatttt tctgccgata tattgtttgc
    26401 cttcctaatg ctgatgattt tcgtgcgttc gttcgacacg aaacaagagc acggtatttc
    26461 gcccaaaccg acatacagcc gcatcaaagc caattatttc agcttcggtt attttgtcgg
    26521 acgcgtgttg ccgtatcagt tgtttgattt aagcaagatt cctgtgttca aacagcctgc
    26581 tccaagcaga atcgggcaag gcagtattca aaatatcgtc ctgattatgg gcgaaagcga
    26641 aagcgcggcg catttgaaat tgtttggcta cgggcgcgaa acttcgccgt ttttaacccg
    26701 gctttcgcaa gccgatttta agccgattgt gaaacaaagt tattccgcag gctttatgac
    26761 ggcagtatcc ctgcccagtt tctttaacgt cataccgcat gccaacggct tggaacaaat
    26821 cagcggcggc gataccaata tgttccgcct cgccaaagag cagggctatg aaacgtattt
    26881 ttacagtgct caggctgaaa accaaatggc aattttgaac ttaatcggta agaaatggat
    26941 agaccatctg attcaaccga cgcagcttgg ctacggcaac ggcgacaata tgcccgatga
    27001 gaagctgctg ccgctgttcg acaaaatcaa tttgcagcag ggcaggcatt ttatcgtgtt
    27061 gcaccaacgc ggttcgcacg ccccatacgg cgcattgttg cagcctcaag ataaagtatt
    27121 cggcgaagcc gatattgtgg ataagtacga caacaccatc cacaaaaccg accaaatgat
    27181 tcaaacagta ttcgagcagc tgcaaaagca gcctgacggc aactggctgt ttgcctatac
    27241 ctctgatcat ggccagtatg tgcgccaaga tatctacaat caaggcacgg tgcagcccga
    27301 cagctatatt gtgccgctgg tgttgtacag cccgaataag gccgtgcaac aggctgccaa
    27361 ccaggctttt gcaccttgcg agattgcctt ccatcagcag ctttcaacgt tcctgattca
    27421 cacgttgggc tacgatatgc cggtttcagg ttgtcgcgaa ggctcggtaa cgggcaacct
    27481 gattacgggt gatgcaggca gcttgagcat tcgcgatggc aaggcggaat atgtttatcc
    27541 gcaatgagtg gcgtaaaaac caataaagac aaatttagat gatgtcgggg aagatgcccg
    27601 accgacaaga ctatgcaaaa tatgaaaaac caaatacgcg gatcaggcat ggatgcacga
    27661 tccaacccgg ccaatgtttc agacggcctg caaaacagtt cgggccatat cggtaccaac
    27721 acgcgctacc gtctgaccaa actcggcgaa caaatggcgc gcctgcccat cgacccgaaa
    27781 atcgcgcgca ttttgttggc ggcgaagaag cacgactgca tggcggaaat attggtgatt
    27841 gcgtccgcgc tatcgattca agacccgcgc gagcggccgc tggaggcacg cgatgccgcc
    27901 gccaaggcgc atgaacgctt taccgacaag caatccgatt tccttgccta cctgaatatt
    27961 tgggacagtt tccagcgcga gcgcgacaaa ggtttgtcca acaagcagct ggtgcaatgg
    28021 tgccgccaat atttcctgtc gcacctgcgg atgcgcgagt ggcgcgagct gcaccaccag
    28081 cttgcccaaa ccgcgattga aatgggtttg accaccaagg aagcggcttt cagacgactt
    28141 tccgaaatca agcagcttac gtcgtctgaa aaccaaggcg atcaagactt atccgccaaa
    28201 cgcaaacaaa aacaactgga taagaaacaa caccgcgccc aaatccgcgc cgccaaagaa
    28261 gcgggctacg aacaaatcca ccgcgccctg ctcaccggcc ttatcgccaa cgtcggcatg
    28321 aaatcgcccg acggcaacga ctataccggc gcgcgcggca gccgtttcca ccttttcccc
    28381 gcctccgccc tgttcaaagc caaacccaaa tgggtgatgg cggcagaact cgttgaaacg
    28441 acaaaacttt atgcgcgcga cgtcgccgtt atccagcccg aatggattga gcaggaagcc
    28501 ccgcacctcg tccgctacca ctatttcgag ccgcactggg aacaaaaacg cggcgaagtc
    28561 gtcgccagcg aacgcgtgac gctttacggc ctgaccgtat tgccgcgccg ccccgtgtct
    28621 tacggcaaag ttgcccccga agaagcgcgc gaaatcttta tccgcagcgc gttggtggcg
    28681 caggaatgcg atttgaaagc ggattttttt gtccacaaca aaaagctgat taaagaaatt
    28741 accgaactcg aacacaaatc gcgcaagcaa gacgtgctgg tcgatgacga agccctgttt
    28801 gcgttttata acgaacgact gcccgaaatg gcttggaaag acgcgcaagg cagcgtttgg
    28861 ggaagtgaag attccgtacg gattattgaa tctgacaaag ccgagaggtc gtctgaaaat
    28921 gagcgcaacg agtttcgtaa aaacaagcgt aatgggtctc gccaaaatga aaatcacggc
    28981 aacaccgtag gttgggttga aaacccaaca tcagccgcaa ctgcaaaaac tgttgggttt
    29041 gacaacccaa cctacgctac tcagcaaccc accccctccc ccgagaggga gggtcgggga
    29101 gagggcaaaa cagttgccgc acaaaccaac ttttccgcaa ccgcagcaaa ccctctcccg
    29161 caggagaggg aacagagtgc ctcagcttca acgttttcag acgacctgcg tcctgcaaat
    29221 ctgcagcaaa ccgccccctc ccccgtgggg gagggctggg gagagggcaa aacagttgcc
    29281 acacaaacca acttttccgc aacctcaaca aaccctctcc cgcaggagag ggaacagagt
    29341 gcctcagctt caacgttttc agacgacctg cgtcctgcaa atctgcagca accctctccc
    29401 tcccccgtgg gggagggctg gggagagggc aaaacagttg ccacccaaac caacttctcc
    29461 gcaaccacag caaaccctct ccctaaccct ctcccacagg agggggaaca gagtgccgca
    29521 gcttcagcgg tttcaaacga ccctcaaccc aaaaagcagc ctgcacccca aaaaaaccgt
    29581 ctgaaacccc tacccctcgc cgacatccgc accttccaag cctggctcaa aaccgccgag
    29641 cgcgacaatc cgcgcctgct gttcctcagc cgcgacgatc tgatgcaaca cgccgccgca
    29701 cacattaccg aagaacagtt ccccaaattc tggcaaaccg cagacggcaa attcaaactt
    29761 tcctaccgct tcgagccgca ccatccgcta gacggcgtga ccatgaccgt gccgctgacc
    29821 gtcctcaacc gcctgcacgc gccgtcgctc gaatggctgg tgcccggcat gatacgcgaa
    29881 aaaatccagt tgcaaatcaa agcactgccc aagcaaatcc gccgcatctg cgtgcccgtg
    29941 cccgaattca tcacccaatt tttaagccaa aaccccgacc gcaacgcccc catcctgccc
    30001 caactcgccc aagccatcgc caaaaccgca ggcgacatcc gcatattcga gcaaatcaac
    30061 caagacgaat gggccgcgtt caggctgccc gaacactgct atttcaacct ccgcattatc
    30121 gacgacggcg gacaagagct tgccggcggc cgcaaactgc acgaattgca acaacaactc
    30181 ggtcaagccg ccgccgttac cttccgcgac aacacccaag aatttgagcg cgacaacgtc
    30241 accgcatggg acatcggcac cctgcccgaa tccatcaaat tcgcacgcgg caaacaacag
    30301 ctcaccggct atctcggcct acaaaaagaa aaagacggcc gcatcgccct gcgcctgttt
    30361 gataccacag aagccgcaga gcaggcacac cgtcaaggtg tcatcgaatt gatgaagctg
    30421 caattaaaag agcaggtaaa ggatttgaac aaaggcatcc aaggcttcac ccaagctgcc
    30481 atgctgctca aacacatcaa cgccgacact ctgcgcgacg acctcaccca agccgtctgc
    30541 gaccgcgcct ttatcggcga agacgagctg ccgcgcaacg aaaaagcctt caaagaacaa
    30601 atcaaacgcg cccgcagccg cctgcccgcc gtcaaagaag ccctcagccg ctacctgcag
    30661 gaaaccgccg ccgtctacgc cgaactcaac agcaaactcg gcaaacaccc attgacccac
    30721 cttctaagac tacgcctgca aaccctgctc gccgccggct tcgccacccg aaccccgtgg
    30781 gcacaatggc cgcgcctccc catctacctc aaagccatga ccctgcgcct cgaaaaatac
    30841 agcagcaacc ccgcccgcga cgcagcccgc gaagccgata tccaagagct ggaacaaatg
    30901 tggcaggaaa aaacagacag cctgattaaa caaggtctcc ccatttcaga cggcctcgcc
    30961 gcgtttaaat ggatgattga agaattgagg gtgtcgctgt tcgcgcagga attgaagaca
    31021 ccgtatccgg tgtcggtgaa gcggctgttg aaagagtggg aaaaaattga aaaataaaaa
    31081 aacagcctga aaagtttcag gctgtttttt tatttgacta atcgaagttt cctatatcta
    31141 tttaagtccc tctcaactaa tccaaaagtt aaatcagcaa catctttggg ggatacgttt
    31201 aaattttcag caatctgttc aataccaatg ccatcatttt ttaaaatagt aagcatttta
    31261 cgtaatgcgc ttgatatttc cctttccatt ggctctggtt cgatagttcg atattttttc
    31321 tttgcaaaca aaggacaaag attgtgtata tacatcctat cagtaatcat tcctaattta
    31381 tgcatccgat atgctaaggc aacaagtgat acaccaaatc gtcttttgat ttttaataaa
    31441 ttttcaatag tgataggaac atgacgatat aagcgtagtg cagcctccgg cattaaaaaa
    31501 gctgaagcaa aggcattagc ctctttttcg ataatatcac gaggttcatc ttctgtaatt
    31561 tcactatttt tactatgttc catactgtat ttatcacgga ttaagtgccc taattcatgg
    31621 gcagcatcaa atcgactacg ttctgcagat ttttgtgtat ttaaaaatac aaatggatga
    31681 ttttcatacc aagtacaaaa ggcatcaatg tcctttgtat ctaaagataa tgaaaataca
    31741 cgaacaccct tagcttcaag tagggtgatc atattcggaa taggttcatt gccaagcccc
    31801 cattctaatc ttagttcctg agcagcctct tcaggagaaa tatcagaaaa atcaggcaat
    31861 acggcttgac ttagtgtaaa ttctgtctcg agccagtcat ttaacaaaaa agccgtaatg
    31921 ctatgattta atgcttgttt ttcaagcctc ttcgaggtgc gtgaacgagc acgaaaactt
    31981 actgcctgag atttcaactc aggcagtctt tcgtcattag taaagaaatg aactggaaac
    32041 tctaataaat tggctaattc atttaaatca ggtatttgct catcttttac atagtttcta
    32101 acctgtcgag cggtaatacc taataactca gctaattttg tttgcgtaca accacgttta
    32161 tccagcgcaa attccagtct ctcacgatta aatgtctgca tgatttatca ttcaaattat
    32221 cctgcttttt gttttctttc aaccaaaggc tcatattctt caacaggttg cttacgttca
    32281 agctcatcaa atttagttaa atcaacatca gctaatataa ttcgctgctt gtacccagtt
    32341 atttgatgac taacaaaacc actcggtaaa gataattcaa gttgcacttt attatacttc
    32401 cagtgaaaca gcagaaccca aaactgcaca gtatcaggca aatctagttt tgaattacga
    32461 atagcctcct caaatccctt acctttcctt gcggttgtca ttggcatccc gtgatgccta
    32521 ccaacatctg aagtagcagt agccacaata atacttttag ttcgacatgg cgagagacat
    32581 agaaatgcac cacccgacca aggctcaagc gtccagccat ctttacttaa atataccctt
    32641 agagcaaatg taatttctgc ttgccgatac atccccaatg tatttctgtc agataatgct
    32701 gctttgtcct gaatattatt atgcgcagta agtacaatct ctttaagcat ctcctgagat
    32761 aagtacttgc tgatttcact taaagcaata tcactatttt gttgctcgac tatttctcct
    32821 acttcaaatg ggaaaggttc tgataatgca aattccacca taaaaatttc ctaattttat
    32881 acgtaatgtt tacacaatat atcaggaaat atgaaaacgt acaactatat ctataaagca
    32941 attaataagt agcctgccca accgtgtcct tatctttcgg cacacccgac ctgcaaatca
    33001 cgcaaaactt ggaatccgtg tgtagggtgt gtgcggtaca tacgcacgca gtctttttaa
    33061 accacagccc ttcccaacta aaccaaaagg tcgtctgaac cctattttca gacgaccttt
    33121 tgccactttg taaaacaaat cttcccacca tcctctcccc aaacatcgcc cgaaccagta
    33181 aacttctcat atttcaacaa ctccttggaa gcaaaccatg tctggtatct acctaccccg
    33241 cctattcccg ccccatatcg ccgaacgcgg cctgttgtat tttcagcagg gcaaggttct
    33301 cgatgtccga aaaacttccg ccgggcatta tcgggcggag gtgtgcggtt cggaaaacta
    33361 ttgggtatgg ctgaagctgg atagtgattt gtatattaaa gacgaaagct gcgattgccc
    33421 ttatgtctaa aggtgcaaac ataccttaaa ttactatatt acataggcaa aatacaagcc
    33481 tataacgaat tggaaacaaa atgccgtctg aaagcattat aaaatctgtt caccttttca
    33541 gatgagtaat gtacacccct atacaatttt tgctactata ccccataaat ccacggctaa
    33601 agatatcctt attatgtcct atgattgatc gaaacgactt gtaatcggct tagcatcaag
    33661 tgccctattc gagttatccg aatcggataa tatatttaga atggaagggg cagaaaccta
    33721 taggcaatat cagagagaaa aacaaaacca tcccctaaaa aaggcgttgt ctttccattt
    33781 attaaaaaac ttctgtcaat caatgaaata aacccaaacg acccaacgat tgggtttatt
    33841 cttttatcca gaaacaatcc agatacagat tacgagtcat aactataggc ttaatattac
    33901 acgattctca ttccatcaag gcggaaaacc gcacaaatac tgaaacacta tcgatcgatt
    33961 tgtaaacaag cctacttaag taacttgcag tccttatcat ttcctttaaa ataatccagc
    34021 ccgtcactac acgaactggc ggacttcttg caaataaagg ttactagatt ttcattcatc
    34081 ttaataataa aaggattttt atctttatct atggctaccg ccttcaacat gaatttactg
    34141 tctaaagccc cgcgcgcgat tccattcaaa cggatacaaa agccttctgc ctctttaatc
    34201 ggcaaacttg gccacttggt agatgtttgt ttaaacctcc cattctgcag ataaaacttt
    34261 tccataaaat gtgcattttc taacaaggct gcccgcactg catttatctt tgctttctca
    34321 acataattgc gatagctcgg ataaacaatt aaagcaagta cagacaatat caagaccact
    34381 gatattaatt caaccagcgt aaacccccga ttatcagtca ttactttact tccaataaga
    34441 acagattatt caacatattt ctttgaacag acttactatc ccattcaaca gtatgcatat
    34501 ttcccactct attttttatc
//
LOCUS       DAWFHY010000013        33101 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.013, whole genome shotgun sequence.
ACCESSION   DAWFHY010000013 DAWFHY010000000
VERSION     DAWFHY010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.013"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(24..149)
                     /gene="ykgO"
                     /locus_tag="WGQ68_000523"
     CDS             complement(24..149)
                     /gene="ykgO"
                     /locus_tag="WGQ68_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HEZ0714384.1"
                     /translation="MQVLSSLKTAKQRHRDCQIVRRKGKVYVICKSNPRFKARQR"
     gene            complement(149..424)
                     /locus_tag="WGQ68_000524"
     CDS             complement(149..424)
                     /locus_tag="WGQ68_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEZ0714385.1"
                     /translation="MKPNIHPDNYRTVLFFDSSANEGWLIRSCAETHGKTMVWTDGKE
                     YPLFSLDTSSASHPVYTGKQRNVNTEGRASKFNQRFQSVMSSFRKDK"
     gene            625..1503
                     /gene="metF"
                     /locus_tag="WGQ68_000525"
     CDS             625..1503
                     /gene="metF"
                     /locus_tag="WGQ68_000525"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HEZ0714386.1"
                     /translation="MNYAKEINALNNSLSDLKGDINVSFEFFPPKNEQMETMLWDSIH
                     RLQTLHPKFVSVTYGANSGERDRTHGIVKRIKQETGLEAAPHLTGIDASPDELRQIAK
                     DYWDSGIRRIVALRGDEPPGYEKKPFYAEDLVKLLRSVADFDISVAAYPEVHPEAKSA
                     QADLINLKRKIDAGANHVITQFFFDVERYLRFRDRCVMLGIDVEIVPGILPVTNFKQL
                     GKMAQVTNVKIPSWLSQMYEGLDDDQGTRNLVAASIAIDMVKVLSREGVKDFHFYTLN
                     RSELTYAICHILGVRP"
     gene            1639..3915
                     /gene="metE"
                     /locus_tag="WGQ68_000526"
     CDS             1639..3915
                     /gene="metE"
                     /locus_tag="WGQ68_000526"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEZ0714387.1"
                     /translation="MTTLHFSGFPRVGAFRELKFAQEKYWRKEISEQELLAVAKDLRE
                     KNWKHQAAANADYVAAGDFTFYDHILDLQVATGAIPARFGFDSQNLSLEQFFQLARGN
                     KDQFAIEMTKWFDTNYHYLVPEFHADTEFKANAKHYVQQLQEAQALGLKAKPTVVGPL
                     TFLWVGKEKGAVEFDRLSLLPKLLPVYVEILTALVEAGAEWIQIDEPALTVDLPKEWV
                     EAYKDVYATLSKVSAKILLSTYFGSVAEHAALLKSLPVDGLHIDLVRAPEQLDAFADY
                     DKVLSAGIIDGRNIWRANLNKVLETVEPLQAKLGDRLWISSSCSLLHTPFDLSVEEKL
                     KANKPDLYSWLAFTLQKTQELRVLKAALNEGRDSVAEELAASQAAAGSRANSSEIHRA
                     DVAKRLADLPANADQRKSPFADRIKAQQAWLNLPLLPTTNIGSFPQTTEIRQARAAFK
                     KGELSAADYEAAMKKEIALVVEEQEKLDLDVLVHGEAERNDMVEYFGELLSGFAFTQY
                     GWVQSYGSRCVKPPIIFGDVSRPEAMTVAWSTYAQSLTKRPMKGMLTGPVTILQWSFV
                     RNDIPRSTVCKQIALALNDEVLDLEKAGIKVIQIDEPAIREGLPLKRADWDAYLNWAG
                     ESFRLSSAGCEDSTQIHTHMCYSEFNDILPAIAAMDADVITIETSRSDMELLTAFGEF
                     KYPNDIGPGVYDIHSPRVPTEAEVEHLLRKAIEVVPVERLWVNPDCGLKTRGWKETLE
                     QLQVMMNVTHKLRAELAK"
     gene            4194..4931
                     /locus_tag="WGQ68_000527"
     CDS             4194..4931
                     /locus_tag="WGQ68_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEZ0714388.1"
                     /translation="MALQDRTGQKVPSVVFRTRVGDTWKDVSTDDLFKGKKVVVFSLP
                     GAFTPTCSSSHLPRYNELFGAFKENGVDAIYCVSVNDTFVMNAWAAEEESDNIYMIPD
                     GNGEFTEGMGMLVGKEDLGFGKRSWRYSMLVNDGVVEKMFIEPEEPGDPFKVSDADTM
                     LKFVAPDWKAQESVAIFTKPGCQFCAKAKQALQDKGLSYEEIVLGKDATVTSVRAITG
                     KMTAPQVFIGGKYIGGSEDLEAYLAKN"
     gene            5180..6583
                     /locus_tag="WGQ68_000528"
     CDS             5180..6583
                     /locus_tag="WGQ68_000528"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0714389.1"
                     /translation="MKKIQADVVVLGGGTAGMGAFRNARLHSDNVYLIENNVFGTTCA
                     RVGCMPSKLLIAAAEARHHALHTDPFGVHLDKDSIVVNGEEVMQRVKSERDRFVGFVV
                     ADVEEWPADKRIMGSAKFIDEHTVQIDEHTQITAKSFVIATGSRPVILPQWQSLGNRL
                     IINDDVFSWDTLPKRVAVFGPGVIGLELGQALHRLGVKVEIFGLGGIIGGISDPVVSD
                     EAKAVFGEELKLHLDAKTEVKLDADGNVEVHWEQDGEKGVFVAEYMLAAVGRRPNVDN
                     IGLENINIEKDARGVPVADPLTMQTSIPHIFIAGDASNQLPLLHEAADQGKIAGDNAG
                     RYPNIGGGLRRSTIGVVFTSPQIGFVGLKYAQVAAQYQADEFVIGEVSFKNQGRSRVM
                     LVNKGHMRLYAEKATGRFIGAEIVGPAAEHLAHLLAWAHQMKMTVPQMLDMPFYHPVI
                     EEGLRTALRDADAKLKA"
     gene            6862..7239
                     /gene="sdhC"
                     /locus_tag="WGQ68_000529"
     CDS             6862..7239
                     /gene="sdhC"
                     /locus_tag="WGQ68_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEZ0714390.1"
                     /translation="MSAKPRPVYLDLPNIRLPIPGIVSILHRISGVGLFIMLPFLLYF
                     LSGTLSQESAFETYRAIVSHPLVKLVLIGVLWAYLHHSLAGIRFLFLDAHKGLELNTA
                     RNTAKAVFASALVLTVVLGALLW"
     gene            7233..7574
                     /gene="sdhD"
                     /locus_tag="WGQ68_000530"
     CDS             7233..7574
                     /gene="sdhD"
                     /locus_tag="WGQ68_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HEZ0714391.1"
                     /translation="MVERKLTGAHYGLRDWAMQRATAVIMLIYTVALLVVLFSLPKEY
                     SAWQAFFDQVWVKVFTQVSFIAVFLHAWVGIRDLWMDYIKPFGVRLFLQVATIVWLVG
                     CLVYSIKVIWG"
     gene            7577..9340
                     /gene="sdhA"
                     /locus_tag="WGQ68_000531"
     CDS             7577..9340
                     /gene="sdhA"
                     /locus_tag="WGQ68_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HEZ0714392.1"
                     /translation="MGFPVRKFDAVIVGGGGAGLRAALQLSKSGLNCAVLSKVFPTRS
                     HTVAAQGGISASLGNVQEDRWDWHMYDTVKGSDWLGDQDAIEFMCRAAPEAVIELEHM
                     GMPFDRVESGKIYQRPFGGHTAEHGKRAVERACAVADRTGHAMLHTLYQQNVRANTQF
                     FVEWTAQDLIRDENGDVVGVTAMEMETGEVYIFHAKAVMFATGGGGRIYASSTNAYMN
                     TGDGLGICARAGIPLEDMEFWQFHPTGVAGAGVLITEGVRGEGGILLNADGERFMERY
                     APTVKDLASRDVVSRAMAMEIYEGRGCGKNKDHVLLKIDHIGAEKIMEKLPGIREISI
                     QFAGIDPIKDPIPVVPTTHYMMGGIPTNYHGEVVVPQGDEYEVPVKGLYAAGECACAS
                     VHGANRLGTNSLLDLVVFGKAAGDSMIKFIKEQSDWKPLPANAGELTRQRIERLDNQT
                     DGENVDALRRELQRSVQLHAGVFRTDEILSKGVREVMAIAERVKRTEIKDKSKVWNTA
                     RIEALELDNLIEVAKATLVSAEARKESRGAHASDDHPERDDENWMKHTLYHSDTNTLS
                     YKPVHTKPLSVEYIKPAKRVY"
     gene            9460..10167
                     /locus_tag="WGQ68_000532"
     CDS             9460..10167
                     /locus_tag="WGQ68_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEZ0714393.1"
                     /translation="MEKMSFEIYRYNPDVDAKPYMQRYELELEPTDVKLLDALVRLKA
                     QDDTLSFRRSCREGICGSDGMNINGKNGLACLTDLRGLKQPVKIRPLPGLPVIRDLIV
                     DMTQFFKQYHSVKPYVVNDNPIDADKERLQTQEERKELDGLYECILCACCSTACPSFW
                     WNPDKFVGPSGLLNAYRFIADSRDTITNERLDNLNDPYRLFRCHTIMNCVDVCPKHLN
                     PTRAIGKIKEIMLKRAV"
     gene            10171..10419
                     /locus_tag="WGQ68_000533"
     CDS             10171..10419
                     /locus_tag="WGQ68_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEZ0714394.1"
                     /translation="MMVFDDIAKRKIRFQTRRGLLELDLIFGRFMEKEFEHLSDKELS
                     EFSEILEFQDQELLALINGHSETDKGHLIPMLEKIRRA"
     gene            10531..11814
                     /gene="gltA"
                     /locus_tag="WGQ68_000534"
     CDS             10531..11814
                     /gene="gltA"
                     /locus_tag="WGQ68_000534"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HEZ0714395.1"
                     /translation="MSKSIKLNVPGQAGLELPVLEASIGHDVVDIRGLTKNTGLFSFD
                     PGFVSTASCESKITYIDGDQGLLYYRGYPIEQLAEKSDYLEVCYLLIYGELPTPGQKA
                     EFDNTVRRHTMVHEQLTWFFRGFRRDAHPMAMMVGVVGALSAFYQDSLDISNPEHRKI
                     AIYRLISKIPTIAAMCYRYSNGLPFNYPKNNLSYSENFLHMMFATPCEDYKPNPVLAR
                     ALDRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGASHGGANEAVLKML
                     DEIGDVSNVAAYMEGVKQRKYRLMGFGHRVYRNMDPRASIMRETCYEVLKELGLEDSP
                     KFKLAMELEQIALKDPFFIERKLYPNVDFYSGIVLSALGIPTEMFTVIFALSRSVGWI
                     SHWHEMISDPSLKIGRPRQLYTGSERRDYVPPGER"
     gene            12019..14847
                     /locus_tag="WGQ68_000535"
     CDS             12019..14847
                     /locus_tag="WGQ68_000535"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEZ0714396.1"
                     /translation="MMDEKLNFSYLFGSNAPYIEELYEAFLENPDAVDEKWKQYFTDL
                     SKQPGTVAVDVAHTPIRESFVTLAKKKIASAVAGGADEAMLKKQVSVLRLISAYRIQG
                     VGAAQLDPLKRIPPRDIEALDPKFHGLSDADMALQFNMGEGDFANRGKLPLSQIISNL
                     KQTYCGHIALEYIYIPNTEERRWVRNYFESVLSTPHYNADQKRRILKEMTAAETLERY
                     LHTKYVGQKRFGVEGGESAIAGLNYLIQNAGKDGVEEVIIGMAHRGRLNVLVNILGKK
                     PGDLFAEFEGRAEIKLPSGDVKYHMGFSSDIATPHGPMHVSLAFNPSHLEIVNPVVEG
                     SARAKQKRLGENGRDKVLPVLIHGDSAFIGLGVNQATFNLSKTRGYTTGGTVHIVINN
                     QIGFTTSDIRDTRSTVHCTDIAKMVSAPVIHVNGDDPECVCFAIQAALDYRKKFHKDI
                     VIDVVCYRKWGHNEGDDPTLTQPMMYKKVSQHPGARALYTEQLIAEGVVTQAEADGYI
                     QAYRDALDKGEHVEQTTLSNFQRTQIDWSKYQGKDWREHIETGLPAADIERLTEKFTA
                     VPEGFALHPTAKRVIEARKAMASGKQAIDWGMAETLAYASLVTKGHGVRISGEDSGRG
                     TFSHRHAVLHDQKREKWDDGTYVPLRHMGEGMGEFLVIDSILNEEAVMAFEYGFACSA
                     PDKLTIWEAQFGDFANGAQVTIDQFLSSGETKWGRLCGLTTILPHGYDGQGPEHSSAR
                     VERWLQLCSENNMQVIMPSEASQMFHLLQRQVLGSYRKPLVIFMSKRLLRFKGAMSPL
                     ENFTEGSTFRPVIGDTAERASNDSVKRVVLCAGQVYYDLEAGRAERKLEDDVAIVRVE
                     QLYPFPYDEVKAELAKYPNAKSVVWAQEEPKNQGAFYQIRHRIEDVISEEQKLSYAGR
                     PSSASPAVGYSSKHIAQLKQLVEDALAL"
     gene            14947..16158
                     /gene="odhB"
                     /locus_tag="WGQ68_000536"
     CDS             14947..16158
                     /gene="odhB"
                     /locus_tag="WGQ68_000536"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEZ0714397.1"
                     /translation="MIIDVKVPMLSESVSEGTLLEWKKKVGEAVARDEILIDIETDKV
                     VLEVPSPQAGVLVEIVAQDGETVVADQVLARIDTAATAAAEAPAAATAAAEAPAAAPA
                     EAAPAAAPAAAQNNAAMPAAAKLAAESGVDVNALQGSGRDGRVLKEDVQNAAAKPAAA
                     AAPAVALPAGARPEERVPMSRLRARVAERLLASQQENAILTTFNEVNMKPIMDLRAKY
                     KEKFEKEHGVKLGFMSFFVKAAVAALKKYPVVNASVDGKDIVYHGYFDIGIAIGSPRG
                     LVVPILRDADQMSIADIEQAIVDYAKKAKDGKIAIEDLTGGTFSITNGGTFGSMMSTP
                     IINPPQSAILGMHATKERAVVENGQVVVRPMMYLALSYDHRIIDGREAVLTLVAIKDA
                     LEDPARLLLDL"
     gene            complement(16210..16326)
                     /locus_tag="WGQ68_000537"
                     /pseudo
     CDS             complement(16210..16326)
                     /locus_tag="WGQ68_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            16523..17956
                     /gene="lpdA"
                     /locus_tag="WGQ68_000538"
     CDS             16523..17956
                     /gene="lpdA"
                     /locus_tag="WGQ68_000538"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0714398.1"
                     /translation="MSQYDVVVIGAGPGGYVAAIRAAQLGFKTACVDAGVNKAGNAPA
                     LGGTCLNVGCIPSKALLQSSEHFHAAQHDFAEHGITVGDVKFDAAKMIERKDAIVTKL
                     TGGVKFLFQKNKVTSLFGTASFAGKNGDAYQIEVDNKGEKTVIEAKHVIVATGSVPRP
                     LPQVAIDNVNVLDNEGALNLTEVPAKLGVIGSGVIGLEMGSVWNRVGSEVTILEAAPT
                     FLAAADQQIAKEAFKYFTKEQGLSIELGVKIGDIKSEGKGVSVAYETAAGEAKTEVFD
                     KLIVAIGRIPNTKGLNAEAVGLEKDERGFIKVDGECRTNLPNVWAIGDVVRGPMLAHK
                     ASDEGVAVAERIAGQKPHIDFNNVPFVIYTDPEIAWVGKTEEQLKAEGVEYKKGTSGF
                     GANGRALAMGKAKGTVKVLADAKTDRILGVHMIGPVVSELLTEGVTALEFFASSEDIA
                     RIIHAHPTLSEVVHEAALAADKRALHG"
     gene            18051..18338
                     /locus_tag="WGQ68_000539"
     CDS             18051..18338
                     /locus_tag="WGQ68_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714399.1"
                     /translation="MNKEIVGVFFIPAGIISMCMAALWQMYVMMTETYTLNRFKDKEL
                     VWRVALLFISFSLAVYLLCPNSRKKGIVFFILGGGGAIMYLLARMWLPFSK"
     gene            18410..19576
                     /gene="sucC"
                     /locus_tag="WGQ68_000540"
     CDS             18410..19576
                     /gene="sucC"
                     /locus_tag="WGQ68_000540"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEZ0714400.1"
                     /translation="MNLHEYQAKELLAGYGLPVQGGILARNGEEAAAAYDKLGGKFAV
                     VKAQVHAGGRGKAGGVKVVKSREEAKEVAESLIGTNLVTYQTDANGQPVNSVLVCEDM
                     YPVQTELYLGAVVDRSTRRITFMASTEGGVEIEKVAAETPEKIFKVTVDPLVGLQPCQ
                     AREVAFQLGLKDKQINEFVKLMTGAYKAFVDNDFALFEVNPLAVRENGALACVDGKIG
                     IDSNALYRLPKIAELRDKSQENERELKASEFDLNYVALEGNIGCMVNGAGLAMATMDI
                     IKLKGGQPANFLDVGGGATKDRVVEAFKLILEDKSVKGVLINIFGGIVRCDMIAEAIV
                     AAVKEINVNVPVVVRLEGNNAELGAKILNESGLKLTSADGLNDAAEKIVAAVNA"
     gene            19587..20477
                     /gene="sucD"
                     /locus_tag="WGQ68_000541"
     CDS             19587..20477
                     /gene="sucD"
                     /locus_tag="WGQ68_000541"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEZ0714401.1"
                     /translation="MSVLINKDTKVLVQGFTGKNGTFHSEQALAYGTKVVGGVTPGKG
                     GQTHLNLPVFNTMKEAVKETGADASVIYVPAPFVLDSIVEAVDSGVGLVVVITEGVPT
                     LDMLKAKRYLETNGNGTRLVGPNCPGVITPGECKIGIMPGHIHTPGRIGIISRSGTLT
                     YEAVAQTTKLGLGQSTCIGIGGDPIPGMNQIDALKLFQEDPDTDAIIMIGEIGGTAEE
                     EAAEYIQSNVTKPVVGYIAGVTAPKGKRMGHAGAIISGGKGTAEEKFAAFEKAGIAYT
                     RSPAELGTTMLEVLKAKGLA"
     gene            complement(20675..21052)
                     /locus_tag="WGQ68_000542"
                     /pseudo
     CDS             complement(20675..21052)
                     /locus_tag="WGQ68_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258178.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(21024..21167)
                     /locus_tag="WGQ68_000543"
     CDS             complement(21024..21167)
                     /locus_tag="WGQ68_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714402.1"
                     /translation="MARYRRNFIAGGTFFFTVKLADPKSRLLVEHIDFAGSLYGCAKT
                     ISL"
     gene            21300..21566
                     /locus_tag="WGQ68_000544"
     CDS             21300..21566
                     /locus_tag="WGQ68_000544"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714403.1"
                     /translation="MKRQTVNQATACVRTAHTLHVDFEVPCNSQVGCVAQPRTRCWVC
                     RLRLAAAETPEEKAKDAAEEAKEEAKDAAEDAKEAAEDAKEEAK"
     gene            complement(21793..22734)
                     /locus_tag="WGQ68_000545"
     CDS             complement(21793..22734)
                     /locus_tag="WGQ68_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HEZ0714404.1"
                     /translation="MEYNAIMKTIAPSHIKPLMLELNQSQRNRIIFIDFYLCFLGKIN
                     RKDLMQAFQISPAVATRDFALYCSSFSKNLRFDTKSKFYLSTEDFEPQFPHSFENAIK
                     ILNGSLIFDIDSQHQPLLPFDLPPVLINPDVLIFSKISKALSLKKPLKISYHSISSGL
                     SEKVIVPLALVNDGFRWHLRAFDRTKNRFADFVLTRIIQANILLAENIEENETINNDI
                     DWNRIVKIELVPHPNHDEAKKVIEMDFGMTNGKLELALRASNIGYVLQQWRVDCSSDH
                     HIDDPAFRLWLLDNLVLYGINNAEHLAPAFDKKRSKP"
     gene            22872..23141
                     /locus_tag="WGQ68_000546"
     CDS             22872..23141
                     /locus_tag="WGQ68_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714405.1"
                     /translation="MKIKFAHLRHPAVGGGYINFAVFDAKSTNGNHSVVLKELTTEAR
                     RNGLKIDQSALIFEQHGRTQFYGDKNLVDFLSANWYPQWTHTLTI"
     gene            23145..23342
                     /locus_tag="WGQ68_000547"
     CDS             23145..23342
                     /locus_tag="WGQ68_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714406.1"
                     /translation="MEMIMTNKIKPGQNTGNNGGIYQEIGSRGGRKDNYATIKDNQKA
                     PPTQNPGSSWIQIKRTPDSSR"
     gene            23357..23527
                     /locus_tag="WGQ68_000548"
     CDS             23357..23527
                     /locus_tag="WGQ68_000548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714407.1"
                     /translation="MFLGIFIALSGILTLLEMFNVISSDMKWGMPLMFTCLGFSIIFD
                     SFKAKQNRKQKE"
     gene            complement(23559..26405)
                     /gene="uvrA"
                     /locus_tag="WGQ68_000549"
     CDS             complement(23559..26405)
                     /gene="uvrA"
                     /locus_tag="WGQ68_000549"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEZ0714408.1"
                     /translation="MCNHHPKHSHGNDTIRIRGARTHNLKNVDLDIPRHKLVVVTGLS
                     GSGKSSLAFDTLYAEGQRRYVESLSAYARQFLQMMDKPDVDLIEGLSPAISIEQKSTS
                     HNPRSTVGTVTEIHDYLRLLYARVGTPYCPEHKLPLSSQTVSQMVDAVLKLPEDTRVM
                     ILAPAVRERKGEFVDFFADLQAQGFARVRVDGEVYQLDEVPKLEKNIKHNIDVVIDRV
                     KVKADIKQRLAESFETALRHGNERALAMEMDSGEEHWFSARFACPVCSYSLPELEPRL
                     FSFNNPMGSCPTCDGLGNTNFFDPEKVVAHPELSLASGAIDGWDKRNQFYFQMIQSLA
                     RHYGFDVQAAWETLPAKIKKVVLHGSGKEVIDFTYLSERGTTFNRSHAFEGIIPNLER
                     RYRETDSETVREKLREYQNHRACPSCGGARLRKEARYVYVSGEPLHEVSAWPLTKTHQ
                     FFETLDLDGNKKQIAEKILKEITERLGFLINVGLDYLNLSRSAETLSGGEAQRIRLAS
                     QIGSGLTGVMYVLDEPSIGLHQRDNDRLLATLKRLRDLGNSVIVVEHDEDAIREADFV
                     VDMGPGAGEHGGNVLIADTPENVAKCENSVTGQYLSGKKSIAVPSERTPVNPDRMLVL
                     KGARGNNLKNVTLELPLGLITCITGVSGSGKSTLINDTLAKITARELNRAQEEPAPYD
                     DIRGLEHLDKVINVDQSPIGRTPRSNPATYTGLFTPIRELFAGVPLSRERGYNVGRFS
                     FNVKGGRCEACQGDGVIKVEMHFLPDVYVPCEVCHGKRYNRETLEIQYKGKNISQVLD
                     MTVEEAREFFDAVPTVSRKLQTLMDVGLGYIRLGQSATTLSGGEAQRVKLALELSKRD
                     TGRTLYILDEPTTGLHFADIALLLEVIGRLKGKGNSIVIIEHNLDVIKTADWIVDLGP
                     EGGDGGGRIIASGSPEEVAKVKGSYTGKYLKNIL"
     gene            26560..27357
                     /locus_tag="WGQ68_000550"
     CDS             26560..27357
                     /locus_tag="WGQ68_000550"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEZ0714409.1"
                     /translation="MNRLYPHPIIAREGWPIIGGGLALSLLVSMCCGWWSLPFWVFTV
                     FALQFFRDPAREIPQNPEAVLSPVDGRIVVVERARDPYRDVDALKISIFMNVFNVHSQ
                     KSPADCTVTKVVYNKGKFVNADLDKASTENERNAVLATTASGREITFVQVAGLVARRI
                     LCYTQAGAKLSRGERYGFIRFGSRVDMYLPVDAQAQVAIGDKVNGVSTVLARLPLTAP
                     QIESEPESEPALQTAPVETAANPSAEQRQIEAVAAKIQAAVQDVLKD"
     gene            complement(28332..30608)
                     /locus_tag="WGQ68_000551"
     CDS             complement(28332..30608)
                     /locus_tag="WGQ68_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0714410.1"
                     /translation="MVQTTLKPIVLSILLINTPLLAQAHETEQSVDLETVSVVGKSRP
                     RATSGLLHTSTASDKIISGDTLRQKAVNLGDALDGVPGIHASQYGGGASAPVIRGQTG
                     RRIKVLNHHGETGDMADFSPDHAIMVDTALSQQVEILRGPVTLLYSSGNVAGLVDVAD
                     GKIPEKMPENGVSGEAGLRLSSGNLEKLTSAGINIGLGKNFVLHTEGLYRKSGDYAVP
                     RYRNLKRLPDSRADSQTGSIGLSWVGEKGFIGVAYSDRRDQYGLPAHSHEYDDCHADI
                     IWQKSLINKRYLQLYPHLLTEEDIDYDNPGLSCGFHDDDNAHAHTHSGRPWIDLRNKR
                     YELRAEWKQPFPGFEALRVYLNRNDYRHDEKAGDAVENFFNNQTQNARIELRHQPIGR
                     LKGSWGVQYLQQKSSALSATSEAVKQPMLLDNKVQHYSFFGVEQAEWDNFTLEGGVRV
                     EKQKASIQYDKALIDRENYYKQPLPDLGAHRQTARSFALSGNWYFTPQHKLSLTASHQ
                     ERLPSTQELYAHGKHVATNTFEVGNKHLNKERSNNIELALGYEGDRWQYNLALYRNRF
                     GNYIYAQTLNDGRGPKSIEDDSEMKLVRYNQSGADFYGAEGEIYFKPTPRYRIGVSGD
                     YVRGRLKNLPSLPGREDAYGNRPFIAQDDQNAPRVPAARLGVHLKASLTDRIDANLDY
                     YRVFAQNKLARYETRTPGHHMLNLGANYRRNTRYGEWNWYVKADNLLNQSVYAHSSFL
                     SDTPQMGRSFTGGVNVKF"
     gene            31030..31620
                     /locus_tag="WGQ68_000552"
     CDS             31030..31620
                     /locus_tag="WGQ68_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEZ0714411.1"
                     /translation="MLLFIDNYDSFTYNIVQYFTELGQEVAVRRNDDITLEEIEALNP
                     QYLVIGPGPCSPKEAGISVAAMRHFAGRLPIMGVCLGHQTIGEAFGGRIVRAKTLMHG
                     KVSPVSHSGKGMFKGLPNPVTCTRYHSLVIERGTLPDCLEITAWTADGEIMGVRHKEY
                     AVEGVQFHPEALLTEHGHDMLNNFLVEFQNFKPQKI"
     gene            31684..32742
                     /gene="trpD"
                     /locus_tag="WGQ68_000553"
     CDS             31684..32742
                     /gene="trpD"
                     /locus_tag="WGQ68_000553"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEZ0714412.1"
                     /translation="MITPQQAIERLISNNELFYDEMTDLMRQMMSGKVPPEQIAAILT
                     GLRIKVETVSEITAAAAVMREFATKVPLENAEGLVDIVGTGGDGAKTFNISTTSMFVA
                     AAAGAKVAKHGGRSVSSSSGAADVMEQMGANLNLTPEQIAQSISQTGIGFMFAPNHHS
                     AMRHVAPVRRSLGFRSIFNILGPLTNPAGAPNQLLGVFHTDLCGILSRVLQQLGSKHV
                     LVVCGEGGLDEITLTGKTRVAELKDGKISEYDIRPEDFGIETRRNLDEIKVANTQESL
                     LKMNEVLDGKEGAARDIVLLNTAAALYAGNVAASLSDGISAAREAIDSGRAKSKKEEF
                     VGFQPQQRCHFLGKMELG"
     gene            complement(32894..>33101)
                     /locus_tag="WGQ68_000554"
     CDS             complement(32894..>33101)
                     /locus_tag="WGQ68_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003792345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0714413.1"
                     /translation="TVVVPNAQSATLLPIIRKKVKPDGIVYTDTFRSYDVLDISEFSH
                     LRKFNGIPKEHLGLHLKKCQWHFK"
BASE COUNT         8355 a         7891 c         8577 g         8278 t
ORIGIN      
        1 accgaagcag gaagggcagg gggtcagcgt tggcgcgctt taaaacgcgg attgcttttg
       61 cagatgacgt aaactttgcc tttacgtctg acaatctgac agtcgcggtg gcgttgtttg
      121 gcggttttga gtgaagacag aacctgcatt atttgtcctt tctaaacgat gacatgacgg
      181 attggaaacg ttggttgaat ttgctggcac ggccttcggt gttgacgttg cgctgtttgc
      241 cagtatagac gggatgggat gcggaagaag tatccagcga aaacagcgga tattctttgc
      301 cgtctgtcca aaccatcgtt tttccgtgtg tttcggcaca ggagcggatt aaccagcctt
      361 cattggcgct gctgtcgaaa aaaaggacgg ttcgataatt gtcaggatga atattcggtt
      421 tcatatatcg ccttagttga attgttataa cataacaaac tttatcataa attagaaggc
      481 ttgtacaagg aaatttaact atttttgtaa tatattagaa attttcatga taaatctgaa
      541 aattttgaaa ttgactcatg tttggcgtaa ctttattatg ttgcctgaaa atatcatata
      601 aaagataata aaaggtacgc agccatgaat tatgcaaaag aaatcaatgc gttaaataac
      661 agtctttctg atttgaaagg cgacattaac gtttcgtttg aatttttccc gccgaaaaac
      721 gaacaaatgg aaacgatgct gtgggactcc atccaccgtc tgcaaaccct gcatcccaag
      781 ttcgtatccg taacttacgg cgcaaattcc ggcgaacgcg accgcacgca cggcatcgtc
      841 aaacgcatca aacaggaaac cggcttggaa gccgcgcctc acctgaccgg tatcgacgct
      901 tctcccgacg aattgcgcca aattgccaaa gactattggg acagcggcat ccgccgcatt
      961 gtcgccctgc gcggcgacga gccgcccggt tatgagaaaa aaccgtttta cgccgaagac
     1021 ttggtcaaac tgttgcgctc cgtcgccgac ttcgacattt ctgtagcggc atatcccgaa
     1081 gtgcatcccg aagcgaaatc cgcacaagcc gacctgatta atttgaaacg caaaatcgat
     1141 gcgggcgcga accacgtcat cacccaattc ttcttcgatg tggaacgcta cctgcgcttc
     1201 cgcgaccgct gcgtgatgtt gggcatcgat gtggaaatcg tccccggcat cctgcccgtt
     1261 accaacttca agcagctcgg caaaatggcg caggtaacca acgtcaaaat cccaagctgg
     1321 ctgtcgcaaa tgtatgaagg tttggacgac gaccaaggca cgcgcaatct ggtggcggca
     1381 agtatcgcca tcgatatggt caaagtcctg tcccgcgaag gcgtgaaaga tttccacttc
     1441 tatacgctta accgcagcga gctgacttac gccatttgcc atattttagg cgtgcgccct
     1501 taaagccgaa aacggcttca gacggcatac gaggccgtct gaaaagcaaa acgccgcccc
     1561 atccgagcca ttctgattta caataccgac cgattcggat cgaaccggtc cttacaaaat
     1621 ccaactggag agttcaacat gacaacatta catttctcag gcttcccgcg tgtcggcgcc
     1681 ttccgcgaat tgaaattcgc acaagaaaaa tactggcgca aagaaatcag cgagcaagaa
     1741 ttgctggctg ttgctaaaga cttgcgcgag aaaaactgga aacaccaggc cgctgccaac
     1801 gccgattacg ttgccgcagg cgatttcact ttctacgacc acatcctcga cctgcaagtc
     1861 gccaccggcg cgattcccgc ccgcttcggc ttcgacagcc aaaacctgtc tttggaacaa
     1921 ttcttccaac tggcgcgcgg taacaaagac caattcgcta tcgaaatgac caaatggttc
     1981 gacaccaact accactactt ggtgcctgaa ttccacgccg ataccgaatt caaagccaat
     2041 gccaaacact atgttcaaca actgcaagaa gcccaagccc tcggtctgaa agccaaaccg
     2101 accgttgtag gtccgttgac tttcctgtgg gtgggtaaag aaaaaggcgc cgtcgaattc
     2161 gaccgtctga gcctgttgcc taaactgttg cctgtttacg ttgaaatcct gactgctttg
     2221 gttgaagccg gtgccgagtg gattcaaatc gacgagcctg ctttgactgt cgatctgcct
     2281 aaagaatggg tagaagccta caaagacgtt tacgctactt tgagcaaagt aagtgccaaa
     2341 atcctgctga gcacttactt cggttctgtt gccgaacacg ccgcattgtt gaaatccctg
     2401 cctgttgacg gcctgcacat cgacttggta cgcgctcccg agcaactgga cgcatttgcc
     2461 gactacgaca aagttctgtc tgcaggcatc atcgacggcc gcaacatctg gcgcgccaac
     2521 ctgaacaaag ttttggaaac tgttgagcct ctgcaagcca aactgggcga ccgtttgtgg
     2581 atttccagct cttgctctct gctgcacact ccatttgact tgtcagttga agaaaaactg
     2641 aaagccaaca aacccgacct gtactcttgg ttggcattca ccctgcaaaa aacccaagaa
     2701 ttgcgcgttc tgaaagctgc attgaacgaa ggccgtgatt ctgttgccga agaactggcc
     2761 gccagccaag ctgccgccgg ctcacgcgcc aacagcagcg aaatccaccg tgcagacgtt
     2821 gccaaacgcc ttgccgattt gcctgcaaac gcagaccaac gcaaatctcc atttgccgac
     2881 cgtatcaaag cgcaacaagc atggttgaac ctgcctctgc tgccgacgac caacatcggt
     2941 tctttcccgc aaactaccga aatccgccaa gcacgcgcag ccttcaaaaa aggcgaactg
     3001 tctgccgccg attacgaagc cgcgatgaaa aaagaaatcg ccttggtggt tgaagaacaa
     3061 gaaaaactgg atttggacgt actggtacac ggcgaagccg agcgtaacga catggttgaa
     3121 tacttcggcg aattgttgag cggttttgca ttcacccaat acggctgggt acaaagctac
     3181 ggctcacgtt gtgttaaacc accgattatc ttcggtgacg taagccgtcc tgaagccatg
     3241 actgtcgcct ggtctactta cgcacaaagc ctgaccaaac gcccgatgaa aggtatgttg
     3301 accggccctg taaccattct gcaatggtct tttgtccgca acgatattcc tcgctctacc
     3361 gtgtgcaaac aaatcgcact ggctctgaac gacgaagtat tggatctgga aaaagcaggc
     3421 atcaaagtca tccaaattga cgaacctgcc atccgcgaag gcttgccgct gaaacgcgcc
     3481 gattgggatg cctacctgaa ctgggcgggc gaatccttcc gcctgtcctc tgccggttgc
     3541 gaagacagca cccaaatcca cacccatatg tgttactccg agttcaacga catcttgccg
     3601 gccatcgccg ctatggatgc tgatgtcatc accatcgaaa cttcacgttc cgatatggaa
     3661 ctcttgaccg cgttcggcga attcaaatac ccgaacgaca tcggcccagg cgtttacgac
     3721 atccacagcc cgcgcgtacc gacagaagcc gaagtggagc acctgttgcg caaagccatc
     3781 gaggttgtac cggttgaacg tctgtgggtt aacccggact gcggcctgaa aacacgcggc
     3841 tggaaagaaa ctctggaaca actccaagtg atgatgaacg taacccacaa attgcgtgcc
     3901 gaattggcga aataagccga gaccgtatga ataaataccg tctgaaagcc tttcagacgg
     3961 tattttgtcc tgatttgcgg cgcaagggcg cagttgccgg aaaatctttt cattgcagct
     4021 tgtttttttc taattcggct ttatatgtgg gaaacaggca aatcggagtt gtgtttgata
     4081 gttttaaata atttatatta tttgaactat aaattataca aatcattttg catggggtag
     4141 aatgcccagc gattcacaat tatttctcaa accaatctat taaggagctt aaaatggctt
     4201 tgcaagatcg taccggtcaa aaagtacctt ccgtagtatt ccgcacccgc gtcggcgaca
     4261 cttggaaaga tgtgtctacc gatgatttgt tcaaaggcaa aaaagtagtc gtattctccc
     4321 tgcccggtgc atttaccccg acttgttctt cttcacacct gccgcgttac aacgaattgt
     4381 tcggcgcgtt caaagaaaac ggcgttgacg caatctactg cgtatctgta aacgatacgt
     4441 tcgtaatgaa cgcttgggct gccgaagaag aatccgacaa catctacatg attcctgatg
     4501 gcaacggcga atttaccgaa ggtatgggta tgctggtcgg taaagaagac ttgggcttcg
     4561 gtaaacgctc ttggcgttac tccatgctgg ttaacgacgg cgtggttgaa aaaatgttca
     4621 tcgagcctga agaaccaggc gatccgttca aagtatccga tgcagatact atgctgaaat
     4681 tcgttgctcc tgattggaaa gctcaagagt ctgtggcaat tttcactaaa ccaggttgcc
     4741 aattctgcgc caaagccaaa caagctttgc aagacaaagg tttgtcttac gaagaaatcg
     4801 tattgggcaa agatgcaacc gttacttccg ttcgcgccat taccggcaag atgactgctc
     4861 ctcaagtctt catcggcggt aaatacatcg gcggcagcga agatttggaa gcttacttgg
     4921 ctaaaaactg atagctgttt gcttaaggcg gtttgattaa actgtctgat ataccggata
     4981 gagttattcg ggcggttcta cactaccgct ccgaataact ctatatttat aagaggattt
     5041 ggatattgtt gcactcaatc gaaattttgt ttttatttat ctgaatgatg tttttgattg
     5101 ggaaaatatt taaatgccgt ctgaaaccga tatgttctgt gtcggcaatg tttcagacga
     5161 aaacggaagg acaaagatta tgaaaaaaat tcaagcggat gtcgttgtac tcggcggcgg
     5221 tactgccggt atgggtgcgt ttcgcaatgc ccgtttacat tcggataatg tttacctgat
     5281 tgaaaacaat gtgttcggca cgacctgcgc gcgcgtgggc tgtatgcctt ccaaactctt
     5341 gattgccgcc gcagaggcgc gtcatcacgc attgcatacc gacccgttcg gcgtgcattt
     5401 ggacaaagac agcatcgtcg tcaacggtga agaggtcatg cagcgcgtta aatccgagcg
     5461 tgaccgtttt gtcggctttg tcgttgccga tgtggaagag tggcctgccg acaagcgcat
     5521 tatgggttcg gctaaattta tcgacgagca taccgtccaa atcgacgagc atactcaaat
     5581 tacggcaaaa agtttcgtga ttgctaccgg ttcgcgtccc gtcatcctgc cgcaatggca
     5641 gtctttgggc aatcgtttga ttatcaacga tgacgttttc tcatgggata cgctgcctaa
     5701 gcgcgttgcc gtgttcgggc cgggtgttat cggtttggaa ctgggtcagg cattgcaccg
     5761 tttgggcgtg aaagttgaaa ttttcggttt gggcggaatc atcggcggca tttccgaccc
     5821 cgtcgtttca gacgaggcga aagccgtgtt cggcgaagaa ttgaaactgc atctggatgc
     5881 taaaaccgag gtcaaactcg atgcagacgg caatgtagaa gtccattggg agcaggatgg
     5941 cgaaaaaggc gtatttgttg ccgaatatat gctggcagcc gtgggccgcc gtccgaacgt
     6001 tgacaatatc ggtttggaaa atatcaatat cgaaaaagat gcgcgcggcg tacctgttgc
     6061 cgacccgctg accatgcaga ccagtattcc gcatatcttc atcgcaggcg atgcgtccaa
     6121 ccaactgcct ctgctgcatg aagctgccga ccaaggcaag attgccggcg ataacgcggg
     6181 ccgctacccg aatatcggcg gcggtttgcg gcgcagcacc atcggcgtgg tgtttaccag
     6241 tccgcaaatc ggctttgtcg gtctgaaata cgcgcaggtt gccgcgcaat accaagccga
     6301 cgaatttgtc atcggcgaag tatcgttcaa aaaccaaggc cgcagccgcg tgatgctggt
     6361 aaacaaaggc catatgcgcc tgtatgccga aaaagccacc ggccgcttta tcggcgcgga
     6421 aatcgtaggc cctgccgccg aacatttggc gcacttgttg gcatgggcgc atcaaatgaa
     6481 gatgaccgtt ccgcaaatgc tggatatgcc gttctaccat cccgttatcg aggaaggtct
     6541 gcgtaccgcg ttgcgcgatg ccgatgcgaa attgaaagcc tgaccgatat ggcaaaacaa
     6601 tgccgtctga aattttttca gacggcattt tgtttttggg gatggggtcg gatgctgata
     6661 ccgtgtcggg aagggggcgg caaaactaaa aatctttctt ttaatctgct gtttccacgc
     6721 gtgtttgtca aaatctatca gtttgttttt aaaatacact gttcaaaatg ggataaaaca
     6781 ggtaaattaa cgtttatgta acccagtgta gcaatgggtt tacggttttt gagtcgatat
     6841 ataactacag aggaattgac tatgtctgcc aaaccgcgtc ctgtttatct ggatttgccg
     6901 aacatccgtc tgccgatacc cgggatagtt tccatccttc accgcatcag tggggtcggg
     6961 ctgtttatta tgctgccttt cctgctgtat ttcctgtccg gtaccctgag tcaagagtct
     7021 gcatttgaaa cttaccgtgc cattgtttcc catcctttgg tcaagctggt tttaatcggt
     7081 gtattgtggg cttatctgca ccattctctc gccggtatcc gctttttatt tttggatgcg
     7141 cacaaaggcc ttgagctgaa tactgcgcgc aataccgcta aagccgtatt tgcttctgca
     7201 ttggttttga ctgtcgtttt gggagcgttg ttatggtaga acgtaaattg accggtgccc
     7261 attacggttt gcgcgattgg gcgatgcaac gtgcgactgc ggttattatg ttgatttata
     7321 ccgttgcact tttagtggtt ctattttccc tgcctaaaga atattcggca tggcaggcat
     7381 ttttcgatca agtttgggtg aaagtgttca cccaagtgag cttcatcgcc gtattcttgc
     7441 acgcttgggt gggtatccgt gatttgtgga tggactatat caaacccttc ggcgtgcgtt
     7501 tgtttttgca ggttgccacc atcgtctggc tggtcggctg cttggtgtat tcaattaaag
     7561 taatttgggg gtaagtatgg gttttcctgt tcgcaagttt gatgccgtga ttgtcggcgg
     7621 tggtggtgca ggtttacgcg cagccctcca attatccaaa tccggtctga attgtgccgt
     7681 tttgtctaaa gtgttcccga cccgttcgca taccgtagcg gcgcagggcg gtatttccgc
     7741 ctctctgggt aatgtgcagg aagaccgttg ggactggcac atgtacgata ccgtgaaagg
     7801 ttccgactgg ttgggcgacc aagatgcgat tgagtttatg tgccgcgccg cgcctgaagc
     7861 cgtaattgag ttggaacaca tgggtatgcc ttttgaccgt gtggaaagcg gtaaaattta
     7921 tcagcgtcct ttcggcggcc atactgccga acacggtaaa cgcgcggtag aacgcgcctg
     7981 tgcggttgcc gaccgtacag gtcatgcgat gctgcatact ttgtaccaac aaaacgtccg
     8041 tgccaatacg caattctttg tggaatggac ggcacaagat ttgattcgtg atgaaaacgg
     8101 cgatgtcgtc ggcgtaaccg ccatggaaat ggaaaccggc gaagtttata ttttccacgc
     8161 taaagctgtg atgtttgcta ccggcggtgg cggtcgtatt tatgcttctt ctaccaatgc
     8221 ctatatgaat accggcgatg gtttgggcat ttgcgcgcgt gcaggtattc cgttggaaga
     8281 catggaattc tggcaattcc acccgaccgg tgtagccggt gccggtgtat tgattactga
     8341 gggtgtacgc ggtgagggcg gtattctgct gaatgccgat ggcgaacgct ttatggaacg
     8401 ctatgcgccg accgtaaaag acttggcttc tcgcgacgtt gtttcccgcg cgatggcgat
     8461 ggaaatctac gaaggtcgcg gctgcggtaa aaacaaagac catgtcttac tgaaaatcga
     8521 ccatatcggc gcagaaaaaa ttatggaaaa actgccgggc atccgcgaga tttccattca
     8581 gttcgccggt atcgatccga ttaaagaccc gattcccgtt gtgccgacta cccactatat
     8641 gatgggcggc attccgacca attaccacgg cgaagtcgtt gtgccgcaag gagacgaata
     8701 cgaagtgcct gtaaaaggcc tgtatgccgc aggtgagtgc gcctgtgctt ccgtacacgg
     8761 tgcgaaccgc ttgggtacga actccctgct ggacttagtg gtattcggta aagctgccgg
     8821 cgacagcatg attaaattca tcaaagagca aagcgactgg aaacctttgc ctgctaatgc
     8881 cggcgaactg acccgccaac gtatcgagcg tttggacaat caaactgatg gtgaaaacgt
     8941 tgatgcattg cgccgcgaac tgcaacgctc cgtacaattg cacgccggcg tgttccgtac
     9001 tgatgagatt ctgagcaaag gcgttcgaga agtcatggcg attgccgagc gtgtgaaacg
     9061 taccgaaatc aaagacaaga gcaaagtgtg gaataccgcg cgtatcgagg ctttggaatt
     9121 ggataaccta attgaagtgg cgaaagcgac tttggtgtct gccgaagcac gtaaagaatc
     9181 acgcggtgcg cacgcttcag acgaccatcc tgagcgcgat gatgaaaact ggatgaaaca
     9241 tacgctgtac cattcagata ccaatacctt gtcctacaaa ccggtgcaca ccaagccttt
     9301 gagcgtggaa tacatcaaac cggccaagcg cgtttattga tgcgttttca gacggtcttc
     9361 gcctcaaagg tcgtctgaaa tctaaccata cccacattga actgcttgaa tttataatac
     9421 aaaatcattg ggcagttgat gagaaaagga acacttctca tggaaaaaat gagttttgaa
     9481 atttaccgtt acaacccgga tgttgatgcc aagccttata tgcagcgtta cgagttggaa
     9541 ttggaaccga ccgacgtgaa acttttggat gctttggtac gcctgaaagc acaagacgat
     9601 accttgtctt tccgccgctc ctgccgcgaa gggatttgcg gttctgacgg tatgaacatc
     9661 aacggcaaaa acggcttggc gtgtttgacc gatctgcgtg gcttgaaaca gccggttaaa
     9721 attcgtcctc tgccaggtct gcctgttatc cgtgacctga ttgtggatat gacccagttc
     9781 ttcaaacaat accattccgt caaaccttat gttgtcaacg ataatccgat tgatgcggac
     9841 aaagagcgtc tgcaaactca ggaagagcgt aaagagttgg acggtttgta cgagtgtatt
     9901 ttgtgcgcct gctgttcgac tgcctgcccg tcattttggt ggaaccctga taaattcgtc
     9961 ggtccgtccg gtttgctgaa tgcttaccgt ttcattgcgg acagccgtga taccatcact
    10021 aatgagcgtt tggataatct gaacgaccca taccgtttgt tccgttgcca caccattatg
    10081 aactgcgtag acgtatgtcc taaacacttg aatccgaccc gagccatcgg taagattaaa
    10141 gagattatgt tgaaacgggc cgtttaagaa atgatggttt ttgacgatat tgccaaacgg
    10201 aaaatccgtt ttcaaacccg ccggggattg ttggagttag atttaatctt cggcaggttt
    10261 atggaaaaag aattcgagca tttgagcgat aaagagctgt ccgagttttc cgaaatcctt
    10321 gaatttcaag atcaagaatt gcttgccttg attaacgggc attcggaaac ggacaaaggg
    10381 caccttatcc cgatgcttga aaaaatcaga cgggcatgag gcatctgaaa tctgcctgca
    10441 ggcagatttc aaaatgcaaa agccgtctga aggcaaagaa cgtgctgcgg atgcagtaac
    10501 gtgggttata acttgcaaag gagcaataat atgtccaaat caatcaaact caacgtaccg
    10561 ggtcaggcag gtttggagct gccggtattg gaagccagca tcgggcacga tgtggttgac
    10621 attcgggggc tgacaaaaaa tacaggtttg ttttctttcg accccggatt tgtttcaacc
    10681 gcaagctgtg agtctaaaat tacttacatc gacggcgatc aaggcttgct ttattatcgc
    10741 ggatacccca tcgagcagct ggccgaaaag tccgattatt tggaagtctg ctacctgttg
    10801 atttacggtg aactgccgac tcccgggcaa aaggcagaat ttgacaatac cgtccgccgc
    10861 cacacgatgg tgcatgaaca gctgacttgg ttcttccggg gcttccgccg cgacgcgcat
    10921 ccgatggcga tgatggtcgg cgtggtcggc gcactgtctg cgttctacca agacagcttg
    10981 gacattagca atcccgaaca ccgcaaaatc gcgatttacc gcctgatttc taaaatcccg
    11041 accattgcgg caatgtgcta ccgctattca aacggtctgc cgttcaatta tccgaagaat
    11101 aatctttctt attccgaaaa cttccttcat atgatgttcg ccacgccgtg tgaagactac
    11161 aaacccaatc ccgttttggc acgcgcgctc gaccgcatct ttattttgca tgccgaccac
    11221 gagcaaaacg cctcaacttc aaccgtccgt ctggcaggct cttcgggtgc gaatccgttt
    11281 gcctgtattg ctgccggtat cgcctgcctg tggggtgctt cgcacggcgg tgcgaacgaa
    11341 gccgtgttga aaatgttgga cgaaatcggc gatgtgtcta atgttgccgc atacatggaa
    11401 ggtgtgaaac aacgcaaata ccgtctgatg ggcttcggtc accgcgtgta ccgcaatatg
    11461 gatccgcgtg ccagcattat gcgcgaaacc tgctatgaag ttttgaagga attgggcttg
    11521 gaagacagtc cgaaattcaa actggcgatg gaattggaac agattgcgct gaaagacccg
    11581 ttctttatcg aacgcaaact gtatccaaac gtcgatttct attccggcat cgtcctgtcc
    11641 gcgctgggca tcccgaccga aatgtttacc gtcatcttcg ccctgtcgcg cagcgtgggc
    11701 tggatttcgc actggcacga gatgattagc gatccttcgc tgaaaatcgg ccgcccgcgc
    11761 cagctttata ccggttcgga acgccgcgat tatgtgccgc caggcgagag gtaacataca
    11821 ttaatatatt gtcaaacagg caatatcaga gaaccggatt gtttcccgaa tccgtctgat
    11881 tgtagtcgga tgaaatcaag acaagcaatc cggtttaaaa tagggtagaa taaaatgtct
    11941 tttcagacgg catcagttta gccgtcagga cgcggacttc taccctttgt ttatatttta
    12001 aagaaaagag cgcacgccat gatggacgaa aaactcaatt tctcttacct gttcggttcg
    12061 aacgcaccct acattgagga attgtacgag gcttttttgg aaaaccccga tgcggttgat
    12121 gaaaaatgga agcagtattt caccgatttg agcaaacagc cggggacggt tgctgtcgat
    12181 gtcgcacaca caccgattcg cgaatcattt gttactttgg cgaaaaagaa aattgcatct
    12241 gccgttgcgg gcggtgcgga tgaggcaatg ctgaaaaagc aagtcagcgt tttacggctg
    12301 atttctgcct atcgtatcca aggcgtgggt gcagcccaac ttgatccgct caaacgtatc
    12361 cccccgcgcg atattgaagc cctcgatccg aaattccacg gtctgtctga tgccgatatg
    12421 gcgcttcaat tcaatatggg cgagggcgat tttgccaatc gcggcaaact gcctttgtcc
    12481 caaatcatca gcaacctcaa acaaacctac tgcggccaca ttgcattgga atatatctat
    12541 attcccaata ccgaagagcg ccgctgggta cgcaattatt ttgaaagcgt attgtccaca
    12601 ccgcattaca atgccgatca aaaacgccgt atcttgaaag agatgactgc tgccgagact
    12661 ttggaacgtt atctgcatac caaatatgtc ggtcagaaac gtttcggtgt cgaaggcggc
    12721 gaaagcgcga ttgccggttt gaactacctg attcaaaacg ccggtaaaga cggcgtggaa
    12781 gaagtcatca tcggtatggc gcaccgtggc cgtctgaatg ttttggtgaa cattttgggt
    12841 aaaaaacccg gagatttgtt cgctgagttt gaaggccgtg ccgaaatcaa attgcctagc
    12901 ggcgacgtga aataccacat gggcttcagc tccgacatcg ccactccaca tggtccgatg
    12961 cacgtttctt tggcgttcaa cccgtcacac ttggaaatcg tcaatccggt agtggaaggt
    13021 tctgcacgcg ccaagcaaaa acgtttgggc gaaaacggcc gcgataaagt cttgcccgta
    13081 ttgattcacg gcgactccgc atttatcggt ttgggtgtca accaagcgac attcaatctg
    13141 tctaaaacgc gcggttatac caccggcggt acggttcata tcgtcatcaa caatcaaatc
    13201 ggctttacca cttccgatat ccgcgatacc cgttcaaccg tacactgtac cgatatcgca
    13261 aaaatggttt ccgctccggt tatccatgtg aacggcgatg atcctgagtg tgtttgcttt
    13321 gccatccaag ccgctttgga ttaccgcaaa aaattccata aagacatcgt gatcgacgtt
    13381 gtctgctacc gtaaatgggg ccacaacgag ggcgacgatc cgaccttgac ccaaccgatg
    13441 atgtacaaaa aagtatcgca acacccgggt gcgcgtgctt tgtacaccga gcaactgatt
    13501 gccgaaggcg tggtaaccca agccgaggct gacggttaca tccaagccta ccgtgatgct
    13561 ttggataaag gcgaacacgt cgagcaaaca acgttgagca acttccaacg cacacaaatt
    13621 gactggagca aataccaagg caaagattgg cgcgaacaca tcgaaaccgg tttgcctgcc
    13681 gcagatattg aacgtctcac tgagaagttt accgccgtac cggaaggctt tgccctgcat
    13741 ccgactgcaa aacgtgtgat tgaagcgcgt aaagccatgg catccggcaa acaggccata
    13801 gattggggta tggccgaaac cttggcatac gccagcctcg taaccaaagg tcacggcgtg
    13861 cgtatttccg gtgaggactc gggacgcggt acgttctcgc accgccacgc cgtattgcac
    13921 gatcaaaaac gcgaaaaatg ggacgacggt acttatgttc ccctgcgcca tatgggcgaa
    13981 ggaatgggcg agttcctggt tatcgattcc atcttgaacg aagaggccgt gatggcgttc
    14041 gagtacggct ttgcctgctc cgctcctgac aagctcacca tttgggaagc tcaattcggt
    14101 gacttcgcca acggcgcgca agtgacgatt gaccaattcc tgtcttcagg cgaaaccaaa
    14161 tggggtcgct tgtgcggtct gaccaccatc ctgccgcacg gttacgacgg tcaaggccct
    14221 gagcactctt ctgcacgcgt agaacgttgg ttgcaactgt gttctgagaa caatatgcaa
    14281 gtcatcatgc cgtctgaagc gtcgcaaatg ttccacctct tgcaacgtca agtcttaggt
    14341 tcataccgca aaccgctagt gattttcatg tccaaacgcc tgttgcgatt caaaggggca
    14401 atgagcccgc tggaaaactt caccgaaggt tcgactttcc gtccggttat cggcgatacc
    14461 gccgaacgcg caagcaacga cagcgtgaaa cgcgtggtat tgtgtgccgg tcaggtttac
    14521 tatgacttgg aagccggtcg agccgaacgt aaactggaag atgatgtcgc tattgtccgc
    14581 gttgagcagc tgtatccgtt cccatacgac gaggttaaag ccgaactggc gaaatatccg
    14641 aacgcaaaat ctgtggtttg ggcacaagaa gagccgaaaa accaaggcgc gttctaccaa
    14701 atccgccacc gcatcgaaga cgttatcagc gaagagcaaa aactgtctta tgccggtcgt
    14761 ccaagcagcg catcgcctgc agtgggctac tcaagcaaac acattgctca attgaaacaa
    14821 ttggttgaag acgctttggc attgtaagcc aagtagcatt ccgtctgagg ctgctcaggc
    14881 ggaacaccca tatgcagaat gaaaaacaca caacaggccg tctgaaaggg ccattggaga
    14941 cacaaaatga ttattgatgt aaaagtacct atgctgtctg aaagcgtatc tgaaggcacg
    15001 ctcttggaat ggaagaaaaa agttggcgaa gcagttgccc gtgacgaaat cctgatcgat
    15061 atcgaaacgg acaaagtggt tttggaagta ccttctccac aagccggcgt attggttgaa
    15121 atcgtagcgc aagacggtga aaccgttgtt gccgaccaag ttttggcacg tatcgatacc
    15181 gctgctactg ccgctgctga agcacctgcc gctgctactg ccgctgctga agcacctgcc
    15241 gccgctcctg ccgaagctgc cccggccgcc gctcctgctg ctgcacaaaa caacgccgct
    15301 atgcctgccg ccgccaaact ggctgcagag agcggtgttg acgtgaacgc attgcaaggt
    15361 tccggccgtg acggtcgcgt attgaaagaa gacgtacaaa atgccgctgc caaacctgcc
    15421 gcagccgctg ctcctgctgt tgcacttcct gccggcgcac gtcctgaaga acgcgtacca
    15481 atgagccgcc tgcgtgcccg tgttgcagaa cgcctcttgg cttctcaaca agaaaacgcc
    15541 attctgacta cattcaacga agtcaacatg aaaccaatca tggacttgcg tgcgaagtac
    15601 aaagaaaaat tcgagaaaga acacggcgtg aaactgggct ttatgtcctt cttcgttaaa
    15661 gccgctgttg ccgccctgaa aaaatacccg gttgtgaatg cttctgttga cggcaaagac
    15721 atcgtgtacc acggctactt cgacatcggt atcgcaattg gcagcccacg cggtttggtt
    15781 gtgccaatcc tgcgcgatgc cgaccaaatg agcattgccg acatcgaaca agcaattgtt
    15841 gattacgcga aaaaagccaa agacggcaaa atcgctatcg aagatctgac cggcggtaca
    15901 ttcagtatta ccaacggcgg tactttcggt tctatgatgt ccaccccgat catcaatccg
    15961 cctcaatctg cgattttggg tatgcacgcc actaaagagc gcgctgtggt tgaaaacggc
    16021 caagttgttg tccgtccaat gatgtatctg gctctgtctt acgaccaccg tatcattgac
    16081 ggccgcgaag ctgtattgac cttggtagcc attaaagacg cgttggaaga cccagcccgc
    16141 ctgttgttgg atctgtaatc gtttcagacg gccttttatt tgttaatgaa aaggccgtct
    16201 gaatttttat agtggattaa atttaaacca gtacggcgtt gcctcgcctt gccgtactat
    16261 ctgtactgtc tgcggcttcg tcgccttgtc ctgatttaaa tttaatccac tatatttaga
    16321 tgtagcgtaa tgtagtatcg tgctacaata ggctcaacga acgattgagg ccgtctgaaa
    16381 catttgattc gaatgaatcg gcagatatgg acttttagac ggctttttct taaaaccatc
    16441 aaaacgcagt cattcaaaat aaaaaagaaa cgaaaagtat cgtttttatt ttgagatact
    16501 gctaaaagca aaggatgaca cgatgtctca atatgatgta gtagtgattg gtgcaggccc
    16561 gggcggatac gttgccgcca tccgtgccgc acaactgggt ttcaaaactg cctgtgtcga
    16621 tgcaggcgtt aacaaagcag gcaatgcccc tgcattgggc ggtacttgct tgaacgtagg
    16681 ctgtatccct tctaaagccc tgttgcaatc cagcgaacat ttccacgctg cgcaacacga
    16741 ttttgccgaa cacggtatca ctgtcggcga cgtaaaattc gacgcggcca aaatgattga
    16801 gcgcaaagat gccatcgtga ccaaactgac cggcggcgtg aaattcctgt tccaaaaaaa
    16861 caaagtaacc agcctgttcg gtactgcttc ctttgccggt aaaaatggcg atgcttacca
    16921 aatcgaagtc gataacaaag gcgagaaaac cgttatcgaa gccaaacacg tcatcgtagc
    16981 gaccggttcc gtaccgcgtc cgctgccaca agtcgctatc gacaacgtta acgtattgga
    17041 caacgaaggc gcattgaacc tgaccgaagt acctgccaaa ctcggcgtga tcggttccgg
    17101 cgtgattggt ttggaaatgg gttccgtatg gaaccgcgtg ggttcagaag ttaccattct
    17161 tgaagccgca ccgactttct tggctgccgc cgaccaacaa atcgccaaag aagccttcaa
    17221 atacttcacc aaagagcaag gtctgagcat cgaattgggc gtgaaaatcg gcgacatcaa
    17281 atctgaaggc aaaggtgttt ccgttgctta cgaaactgct gccggcgaag ccaaaaccga
    17341 agtattcgac aaactgatcg ttgccatcgg ccgtattcca aacaccaaag gcctgaacgc
    17401 ggaagccgta ggcttggaaa aagacgagcg cggctttatc aaagtagatg gcgaatgccg
    17461 taccaacctg cctaacgtat gggcaatcgg cgacgtggtt cgcggcccga tgttggcaca
    17521 caaagccagc gacgaaggcg ttgccgttgc cgaacgcatt gccggtcaaa aaccgcatat
    17581 cgacttcaac aacgtaccgt tcgtgattta caccgatcct gaaatcgctt gggtgggtaa
    17641 aaccgaagag cagctcaaag ccgaaggcgt ggagtacaaa aaaggtactt caggttttgg
    17701 tgcgaatggt cgcgcattgg caatgggcaa agccaaaggt acggttaaag tgttggcaga
    17761 cgccaaaacc gaccgcatct tgggcgtaca catgattggt ccggttgtca gcgaattgct
    17821 taccgaaggc gtgactgcgc tcgaattctt cgccagcagc gaagacatcg cccgcattat
    17881 ccatgcccac ccaaccttgt ccgaagtggt tcacgaagct gcattggcgg ccgacaaacg
    17941 cgctttgcac ggttgataga cattaaggcc tccgacaaat tgaatgttcc gagagcttcg
    18001 ttttctgatt tataattccg tcaggcaaac aaacagcatt tacattcatt atgaacaaag
    18061 aaatagtcgg tgttttcttt ataccggcgg gcatcatcag catgtgtatg gccgcattgt
    18121 ggcagatgta tgtgatgatg accgaaactt atacgctcaa ccgtttcaaa gataaagaat
    18181 tggtttggcg cgtggcattg ttgtttatca gtttcagcct tgccgtttac ctgctctgtc
    18241 cgaattcgcg taaaaaaggc atcgtctttt ttattctcgg gggaggcggc gcaatcatgt
    18301 atctgctggc gcggatgtgg ttgcctttca gtaaatagta gggccgtctg aaaatatggg
    18361 attggccgca gggcgattcc taagacccac tcaccttaag gagaaatcca tgaatctaca
    18421 cgagtatcag gctaaagaac tgctggctgg ctacggtttg cccgtacaag gcggtatttt
    18481 ggcacgcaac ggcgaagaag ccgctgcagc ttacgacaaa ttgggcggta agtttgccgt
    18541 tgtcaaagca caagtacacg ccggcggccg cggtaaagcg ggcggcgtaa aagtcgtcaa
    18601 aagccgcgaa gaagccaaag aagtggctga aagcctgatt ggcaccaact tggtaactta
    18661 ccaaaccgat gccaacggcc aacctgtcaa cagcgttttg gtttgcgaag acatgtatcc
    18721 tgttcaaacc gagctgtatt tgggcgcagt ggtcgatcgt tctacccgcc gcattacatt
    18781 catggcttct accgaaggcg gcgtggaaat tgaaaaagtt gctgccgaaa ctcctgaaaa
    18841 aatcttcaaa gtaaccgttg atcctctggt cggcctgcaa ccttgccaag cgcgcgaagt
    18901 ggcgttccaa ctgggcttga aagacaaaca aatcaacgag ttcgttaaac tgatgaccgg
    18961 tgcgtacaaa gcgtttgtcg acaacgactt cgccctgttt gaagtaaacc cgctggcagt
    19021 tcgcgaaaac ggcgcactgg cttgcgtgga cggcaaaatc ggcatcgaca gcaacgcgct
    19081 ctaccgcctg ccaaaaatcg ctgaattgcg cgacaaatct caagaaaacg aacgtgagct
    19141 gaaagcttct gaattcgacc tgaactatgt tgccctggaa ggcaacattg gctgtatggt
    19201 taatggtgca ggtttggcga tggccactat ggacatcatc aaactcaaag gcggccaacc
    19261 tgccaacttc ttggacgttg gcggtggcgc aaccaaagac cgcgtggttg aagcgttcaa
    19321 actgattttg gaagacaaat ccgttaaagg cgtattgatc aacatcttcg gcggtatcgt
    19381 acgttgcgac atgattgcgg aagccatcgt ggcagccgtt aaagaaatca acgtcaacgt
    19441 tcctgtcgtt gttcgtttgg aaggtaacaa cgccgaactc ggcgcgaaaa tcctgaacga
    19501 atcaggtctg aaactgactt ctgcagacgg cctgaatgac gcagccgaaa aaattgttgc
    19561 agccgtaaac gcctaaggag aaaagaatga gcgtattgat taataaagac accaaagtat
    19621 tggttcaagg tttcaccggt aaaaacggta ctttccactc cgaacaagct ctggcttacg
    19681 gcactaaagt tgtcggcggc gttaccccag gcaaaggcgg tcaaacccac ctgaacctgc
    19741 ctgtattcaa caccatgaaa gaagccgtta aagaaaccgg cgcggacgca tctgtcattt
    19801 acgttccagc tccgtttgtt ttggactcta tcgttgaagc cgttgattca ggcgtaggct
    19861 tggtcgttgt gattaccgaa ggcgtgccca ctttggacat gctcaaagcc aaacgttact
    19921 tggaaactaa cggcaacggt acccgcttgg tcggccctaa ctgcccgggc gtgattactc
    19981 cgggtgagtg caaaatcggt attatgccgg gccacatcca cactccgggc cgcatcggta
    20041 ttatttcccg ttccggtaca ttgacttacg aagctgtggc acaaaccacc aaactgggct
    20101 tgggtcaatc aacctgtatc ggtatcggcg gcgacccgat tcctggtatg aatcaaatcg
    20161 acgcactgaa actcttccaa gaagacccgg ataccgacgc catcatcatg atcggtgaaa
    20221 tcggcggtac tgcggaagaa gaagcagccg aatacatcca atccaacgta accaaacctg
    20281 ttgtaggtta cattgctggt gttaccgctc ctaaaggcaa acgcatgggc cacgctggtg
    20341 cgattatttc cggcggcaaa ggtactgccg aggaaaaatt cgccgcgttt gaaaaagccg
    20401 gtatcgccta tactcgcagc cctgccgagt tgggcacgac gatgctggaa gtgttgaaag
    20461 caaaaggttt ggcataatca ggtttgacaa ctgattgaac atcaaatgcc gtctgaaacc
    20521 ggaaatcggg tttcagacgg cattttgttt gtcatttcaa aaagaggcag cctcaacata
    20581 cccacattaa tttttgccct ttgaggcagt cagaatattt atcggtcaaa caggagcaaa
    20641 cccgtaagtc aggttgcaca cgccttgttt gaagtcaggg aatgataagt atcgcatttg
    20701 aaaagactga tattattttt tgcaggattg ttccggtttc tgtttcagcc cgcgatattc
    20761 tttgtttgat aggattggaa tggatatagt ccgcacgatg ctgcaaatcc gtttcatcgc
    20821 gcacggtgtg ttcgtaaaaa cgccgttgtc aaataccgtg ttcgtgccgc cgctgtttgc
    20881 cggcggataa gtttttagca tgaggggaat atgcggagaa tttggtttta atcagccgcc
    20941 ggagtaggga ataatccgca tcgtcgggtg gcagcgtcca aatggcgtga atgtggttcg
    21001 gcagcacgca tacggcgacg gtttcaaagg gatattgttt ttgcacatcc atataagctg
    21061 cccgcgaagt cgatatgttc gacaagcagg cgcgatttcg ggtcggcgag tttgacggtg
    21121 aaaaagaatg tgccgccggc aatgaagttt ctgcgataac gcgccatagg atttttgttt
    21181 gtggtttggg tgttggagaa ggaatggttt gttgtttaag gttttaactt tgttggagct
    21241 gatgttttca gacgacattg gaggccgtct gaaagcgcta gcttcaacga agttaaaagt
    21301 tgaaacggca aaccgtcaat caagcaaccg cgtgcgtgcg taccgcacac accctacacg
    21361 ttgattttga agttccgtgt aattcacagg tagggtgtgt ggcgcagcca cgcacgcggt
    21421 gttgggtatg caggctacgg cttgctgctg cagaaactcc tgaagaaaaa gccaaagacg
    21481 ccgctgaaga ggctaaagaa gaagcaaaag acgcagcgga agatgccaaa gaagctgctg
    21541 aagacgctaa agaagaagct aaataattcg gatagcttta cagaaaaacg gattccaatg
    21601 attgggaatc cgttttttgt tttgtgagtt agggtagttg ttttagataa agctttttca
    21661 gttttcagac ggccattttt catgccgtct gaaagtgtta gttttaacta agttcaattt
    21721 acaacagaaa gccatcaatc aaacaaccgc gtgtgtggcg cagccacgca cgcggtcggt
    21781 tggatatgta ggctacggct tgctacgttt tttatcaaag gcaggcgcta agtgttcagc
    21841 attgttgatg ccgtataaaa ctaaattatc taacagccat aatctaaaag caggatcatc
    21901 gatatgatga tctgacgaac aatcgactcg ccattgctgt aaaacatatc cgatatttga
    21961 agctctaagc gccaattcta attttccatt tgtcattcca aagtccattt caatcacttt
    22021 tttagcttca tcatgattag gatggggaac caactcgatt ttaacaattc tattccaatc
    22081 aatatcattg ttaatcgttt cattttcttc aatattttca gctaataaaa tatttgcttg
    22141 aattattcgt gttaggacaa aatctgcaaa tcggtttttt gttctatcga atgcccgtaa
    22201 atgccatcga aatccgtcat taactagagc aagaggcaca ataactttct cagataatcc
    22261 agatgaaatt gaatgatatg agatttttag aggttttttt aaggataatg ctttcgatat
    22321 tttagaaaag attaagacat caggatttat taaaacagga ggtaaatcaa acggaagaag
    22381 aggttgatgt tgggaatcaa tatcaaatat taaactgcca ttaagaattt taattgcatt
    22441 ttcaaatgaa tgggggaatt gaggttcaaa gtcttcagta gagaggtaaa atttactttt
    22501 ggtatcaaaa cggagatttt tcgaaaaact agagcaatat agcgcaaaat ccctagtagc
    22561 aacagctggg gaaatttgaa atgcctgcat caaatctttt ctatttatct ttcctagaaa
    22621 gcatagatag aaatcaataa atataatcct attgcgttga gattggttca actctagcat
    22681 aaggggcttt atatgggaag gagcgattgt tttcataatt gcattatatt ccatcaatct
    22741 ttttcatggc aatatgaatt caaaaaaatt gcttgacgta atcaaaaaga gtgcatataa
    22801 tgcaaaaagc tacttcatag tagctctctg acttaaaatt tctgttatgt aattttttat
    22861 aggatttatt tatgaaaata aaatttgctc acttaagaca tccagccgta ggtggaggat
    22921 atattaactt tgctgttttt gatgctaaat caactaatgg taaccatagt gtcgtactga
    22981 aggaattgac cactgaagct cgtagaaatg gattaaaaat tgaccaatct gcgttaatat
    23041 ttgaacaaca tgggcgtaca caattctatg gagacaaaaa tctagtagat tttttatctg
    23101 caaattggta tccgcaatgg actcatactt taactattta aattatggag atgataatga
    23161 cgaacaaaat caaacctggt caaaatacag gaaataacgg tggaatttat caagaaattg
    23221 gttcacgagg tggtcgaaaa gataactacg caaccattaa agataatcaa aaagcaccac
    23281 ccactcaaaa tccaggaagc tcatggattc aaattaaaag aactccagat agctccagat
    23341 aataaggata gttctaatgt ttttaggtat ttttatagct ttatcaggca ttcttacatt
    23401 attagaaatg tttaatgtta tttcttcgga tatgaaatgg gggatgcctt taatgttcac
    23461 ctgtttggga tttagcatta tttttgatag cttcaaagcc aaacaaaatc gaaaacagaa
    23521 ggaataaaaa aacgggatgg tttaaaccat cccgtttttt aaagtatatt cttcaaatac
    23581 ttcccagtat aactcccctt aaccttcgcc acttcctccg ggctacctga agcaataatc
    23641 ctccctccgc cgtcgccgcc ttctggccct aagtccacaa tccaatcggc ggttttaatc
    23701 acatccagat tatgctcgat aatcactatc gagttgcctt tgcctttcag acgacctatg
    23761 acttccagca gcagggcgat gtcggcgaag tgcaggccgg tggtgggttc gtcgaggata
    23821 tagagcgttc tgccggtgtc gcgtttggag agttccaagg cgagtttgac gcgctgggct
    23881 tcgccgccgg agagggtggt ggcggattgg ccgaggcgga tgtagccgag gcctacgtcc
    23941 atcagggttt gcagtttgcg cgatacggtg gggacggcgt cgaaaaattc gcgggcttct
    24001 tcaacggtca tgtcgaggac ttggctgatg tttttgcctt tgtattggat ttcgagcgtt
    24061 tcgcggttgt agcgtttgcc gtggcagact tcgcagggaa catacacgtc gggcaggaag
    24121 tgcatttcga ctttgatgac gccgtcgcct tggcaggctt cgcagcggcc gcctttgacg
    24181 ttgaaggaga atctgccgac attgtagccg cgttcgcgcg agaggggtac gccggcgaag
    24241 agttcgcgga tgggggtgaa caggccggtg taggtggcgg gattggagcg tggggtgcgg
    24301 ccgatggggg attggtcgac gttgatgact ttgtcgaggt gttcgaggcc gcggatgtcg
    24361 tcgtatgggg cgggttcttc ttgggcgcgg ttgagttcgc gggcggtgat tttggcgagg
    24421 gtgtcgttaa tcagggtgga cttgccgctg ccggatacgc cggtgatgca ggtaatcaaa
    24481 ccgagcggca gttcgagggt aacgtttttg aggttgttgc cgcgcgcgcc tttgaggacg
    24541 agcattcggt cgggattgac gggtgtgcgt tcagacggca cggcaatgga ttttttgccg
    24601 ctgaggtatt gtccggtaac ggagttttca catttggcga cgttttcggg cgtgtcggca
    24661 atcaatacgt tgccgccgtg ttcgcctgcg cccgggccca tatcgacgac gaaatcggct
    24721 tcgcggatgg cgtcttcgtc gtgttcgact acaatcacgc tgttgcctaa atcgcgcagg
    24781 cgtttgaggg tggcgagcag gcggtcgttg tcgcgctggt gcaggccgat ggagggttcg
    24841 tccaaaacgt acatcacgcc ggtcaggccg ctgccgattt ggctggcgag gcggatacgc
    24901 tgggcttcgc cgccggagag ggtttcggcg gagcggctta aattcaggta atccagcccg
    24961 acgttaatca aaaagccaag ccgctcggtg atttctttga ggattttttc ggcgatttgt
    25021 tttttgttgc cgtccaaatc cagcgtttca aagaattggt gggttttggt cagcggccag
    25081 gcggagactt cgtgcaacgg ttcgccgctg acgtaaacgt agcgcgcttc tttgcgcaaa
    25141 cgtgcaccgc cgcagctcgg gcaggcgcgg tggttttggt attcgcgcaa tttttcgcgc
    25201 acggtttcgc tgtcggtttc gcggtagcgg cgttcgagat tgggaatgat gccttcgaag
    25261 gcgtggctgc ggttgaaggt ggtgccgcgt tcggataggt aggtgaaatc aatgacttcc
    25321 ttgcccgaac cgtgcaacac gacttttttg atttttgcag gtagggtttc ccaagcagcc
    25381 tgcacatcaa aaccgtaatg ccgtgccagc gactgaatca tttggaaata gaactggttg
    25441 cgtttgtccc agccgtcaat cgcgcccgaa gccagcgaca attcggggtg ggcgaccact
    25501 ttttcggggt cgaagaaatt ggtgttgccc aagccgtcgc aagtcgggca ggaacccatc
    25561 gggttgttga aggaaaacag gcgcggctct aattctggca ggctgtacga acatacgggg
    25621 caggcgaaac gcgcggaaaa ccaatgttct tcgccgctgt ccatctccat cgccagcgcg
    25681 cgctcgttgc cgtggcgtaa cgcggtttca aaactttccg ccagccgctg cttgatgtcc
    25741 gccttcactt ttacacggtc gatgaccacg tcgatattgt gcttgatgtt tttttccagc
    25801 ttcggcactt cgtccaactg atagacttcg ccgtccacgc gcacgcgcgc aaaaccctgc
    25861 gcctgcaagt cggcaaagaa atcgacaaac tcgcccttac gctctcgtac cgccggcgcc
    25921 agaatcatca cgcgcgtgtc ttccggcagt tttaagacgg catcaaccat ttgagatacg
    25981 gtttggctgg atagcggcag cttgtgttcg gggcagtagg gtgtgccgac gcgtgcgtac
    26041 aaaaggcgca gatagtcgtg gatttccgta accgtgccga cggtggaacg cggattgtgg
    26101 ctggtggatt tctgctcgat ggaaatcgcg ggcgacaggc cttcgatcaa atcgacatcg
    26161 ggtttgtcca tcatctgcaa aaactgccgc gcataggcgg acaggctctc gacataacgg
    26221 cgttggcctt cggcatataa agtatcaaac gctagcgacg atttgccgct gcccgacaat
    26281 cctgttacca ccacgagctt gtggcgcgga atgtccaaat cgacgttttt caaattatgc
    26341 gtgcgcgcgc cgcggatgcg gatggtgtcg ttgccgtgcg aatgtttggg atgatggttg
    26401 cacataatgg atgccgcctg aaaaataaag gaaaaccgat attgtagcac tttctcggat
    26461 gccgtctgaa gccgcgttca gacggcattt gtcggcggag tacggcagat tccgctataa
    26521 tgtcggcaat tttaacccgc ttgaacaaaa ggatgacaaa tgaaccgtct ttacccccac
    26581 ccgattatcg cccgtgaggg ctggccgatt attggcggcg gtttggcttt gagcctgctg
    26641 gtgtcgatgt gttgcggctg gtggtctttg ccgttttggg tgtttaccgt atttgccctg
    26701 cagtttttcc gcgaccctgc gcgtgagatt ccgcaaaatc ctgaagcagt gttgagtccg
    26761 gttgacggcc gtatcgtggt ggtcgagcgc gcacgcgatc cgtatcgtga tgtcgatgct
    26821 ttgaaaatca gtatttttat gaacgtgttc aacgtgcatt cgcaaaaatc gcctgccgat
    26881 tgtacggtaa cgaaagtggt ctataacaaa ggcaaattcg tgaatgcgga tttggacaaa
    26941 gccagcacgg aaaacgaacg taatgcggtg ttggcgacta cggcttccgg tcgtgaaatt
    27001 acttttgttc aagtggccgg tttggtggcg cgccgtattt tgtgctacac ccaagcaggt
    27061 gcgaaactgt ctcgcggcga acgctatggc tttatccgtt ttggctcgcg cgtggatatg
    27121 tatctgcctg tcgatgcgca ggcgcaagtg gcgattggcg ataaagtaaa cggtgtcagc
    27181 actgtattgg cgcgtttgcc gctgactgcg ccgcaaatcg aatccgagcc tgaatctgag
    27241 cctgctttac aaactgctcc ggttgaaaca gcggcaaacc catctgccga acaacggcaa
    27301 atcgaggcag tggcggctaa gattcaggcg gctgtgcaag atgtgttgaa agattaattt
    27361 tgcgaactga aatagaaaat atcagtatca tcattcacac gaatgaggaa gtttggtttt
    27421 ttgaattttt gctaatgttc acaccgttat gttcacgaaa atgggaatcc gggaacttaa
    27481 cgttacagcg atttatcgga aacggctgaa accgaacgaa ttggattccc gcctgcgcgg
    27541 gaatgacaac tcattagtta cctaaaactt aaaaaacgga aacctttacg ccgtcattcc
    27601 cacgaaagtg ggaatccggg aacttaacgt tacagtgatt tatcggaaac ggctgaaacc
    27661 gaacgaattg gattcccgcc tgcgcgggaa tgacggcaca ttagttacct aaaacttaaa
    27721 aaaaaaaaac agaaaccttt ccgcctcatt cccacgaaag tgggaatcta gaacccaaat
    27781 gctaaggcga tttatcggaa acggctgaaa ccgaacgaat tggattcccg cctgcgcggg
    27841 aatgacgact tattagttac ctaacactta aaaaacagaa acctttccgc gtcattccca
    27901 cgaaagtggg aatctagaac ccaaatgcta aggcgattta tcggaaacga ctgaaaccga
    27961 atgaattgga ttcccgcctg cgtgggaatg acgacccatt agttacctaa aatttaaaaa
    28021 acagaaacct ttccgcgtca ttcccatgaa agtgggaatc tagaacccaa atgctaaggc
    28081 gatttatcgg aaacggctga aaccgaagag accggattcc cgcctgcgcg ggaatgatgg
    28141 gtttctgctt ttgatttttc tgcttttgcg agaatgacgg cgtgaaagca agaatgatga
    28201 aacaaagaaa atgggaatga tggcatagtg gtttgttctt tgtctttgcc atatttccta
    28261 acaaattgat taaaaagaaa aaaggttttc agaatgccgt ctgaaaacct tttttgtttg
    28321 cctgtccgat tttaaaactt cacgttcacg ccgccggtaa agctgcggcc catttgcggc
    28381 gtatcagaga ggaagctgct gtgggcgtaa acggattggt tgagcaggtt gtcggctttg
    28441 acgtaccaat tccactcgcc atagcgcgta ttgcggcggt agtttgcgcc gaggttgagc
    28501 atatggtgtc cgggcgtgcg cgtttcgtag cgggcgagtt tgttttgggc gaacacgcgg
    28561 tagtagtcca aattggcatc gatgcggtcg gtcagcgagg ctttcaggtg gacgccgagg
    28621 cgcgcagccg gaacgcgagg ggcgttttgg tcgtcctgcg cgatgaaagg acggttgccg
    28681 taggcatctt ccctgccggg tagggaaggc aggtttttca gacggcctcg tacatagtcg
    28741 ccggaaacgc cgatgcggta gcgcggtgtc ggtttgaagt agatttctcc ttccgcgccg
    28801 tagaagtcgg cgccggattg gttgtagcgc acgagcttca tttcgctgtc gtcttcgatg
    28861 gatttggggc cgcgtccatc gtttaaggtt tgggcgtaaa tgtagttgcc gaagcggttg
    28921 cggtagagtg ccagattgta ttgccagcgg tcgccttcgt agcccaacgc gagttcgata
    28981 ttgttggaac gctctttgtt caggtgtttg ttgccgactt caaaagtgtt ggtggcaacg
    29041 tgtttgccgt gtgcgtacag ctcttgcgtt gacggcaggc gttcctgatg ggaggcggtc
    29101 aggctgagtt tgtgttgcgg cgtgaaatac cagttgcccg aaagtgcgaa cgagcgggcg
    29161 gtttggcggt gtgcgccgag gtcgggcagg ggctgcttgt agtagttttc ccgatcaatc
    29221 aatgctttgt cgtactggat ggaagctttt tgtttttcca cgcgtacgcc gccttcaagc
    29281 gtgaagttgt cccattctgc ctgttctacg ccgaaaaagc tgtaatgttg cactttattg
    29341 tcaagcagca tcggttgttt gaccgcttcg gatgtggcag ataaagcact ggatttttgt
    29401 tgtaaatatt gcacgcccca gctgcctttc agacggccta tgggttggtg gcgcaactcg
    29461 atgcgggcgt tttgcgtttg gttgttaaaa aagttttcga ccgcatcgcc tgctttttcg
    29521 tcgtggcggt agtcgttgcg gttcaagtat acgcgcaggg cttcaaaacc ggggaacggt
    29581 tgcttccatt cggcacggag ttcgtagcgt ttgttgcgca ggtctatcca cggtctgccg
    29641 ctgtgggtgt gtgcgtgtgc attatcgtcg tcgtggaagc cgcagctcaa gcccggattg
    29701 tcgtaatcga tgtcttcttc ggtcaacagg tgcggataaa gctgtaaata gcgtttgtta
    29761 atcaagctct tttgccagat gatgtcggcg tggcaatcat cgtattcgtg gctgtgggca
    29821 ggcagaccat attggtcgcg acggtcgctg tacgctacgc cgataaaacc tttttcgcca
    29881 acccaagaca gcccgatgct gcccgtttgc gaatcggcgc ggctgtcggg caggcgtttc
    29941 agattgcggt aacgcggtac ggcgtaatcg cccgatttgc ggtacaagcc ttccgtatgc
    30001 aatacaaagt ttttgcccaa gccgatattg atgcctgcgg atgtcagttt ttccaaattg
    30061 ccgctgctca aacgcaatcc ggcttcgccc gatacgccgt tttcaggcat tttttcgggg
    30121 attttgccat cggcaacatc gaccagcccc gccacattgc ccgagctgta caagagcgta
    30181 accggcccgc gcaggatttc gacctgttgc gacaaggcgg tatctaccat aatggcgtga
    30241 tcgggcgaaa aatccgccat atcgcctgtt tcgccgtgat ggttcaacac tttaatccgc
    30301 ctgcctgttt gaccgcgaat aacgggagcg gacgcgccgc cgccgtattg cgaggcgtgg
    30361 atgcccggta cgccgtctaa agcatcgccc aagttgacgg ctttttggcg caaggtatcg
    30421 ccggagatga ttttgtcgga ggcggtcgaa gtgtgcagca gccccgatgt ggcgcgcgga
    30481 cggcttttgc cgacgacgct gaccgtttcc aagtccaccg attgctcagt ttcatgcgct
    30541 tgggcaagga ggggtgtgtt gattaaaaga attgataaaa caatgggttt gagtgtagtt
    30601 tgtaccattt tggcttctcg actcatttca aaagtttgtt attatataac attgcattcc
    30661 ttatatcata agattttgag aacactcaga gggcataggc aaaagttttt caaatgacac
    30721 ggttgcggca tcgggcggtg tccatttgta tccgccgtca ttcgggggcg cggttgatgt
    30781 tgacgcagat tccgctgtgt ttgcccacta tgttcggctg cgggataatc aaaatttatg
    30841 agtgcataaa aacggcacgt tcccgaacgg tcgaaaaggt ggcaaaatgg cgtacttgtc
    30901 agaacgcgag gctctgcgcc aggttcacga gggcgcatcg ggcacgctaa gatataagag
    30961 ggtatggatg ggggtatcgg aaatgcagtt aataacaaac aaattataaa tcaataggtt
    31021 aatcacaaaa tgcttttgtt tatcgacaat tacgacagtt ttacttacaa catcgtccag
    31081 tatttcactg aattggggca ggaagttgcc gtgcgccgca acgatgatat tacgttggag
    31141 gaaatcgagg cattgaatcc gcaatatctc gttatcggcc ccggcccgtg ttcgcccaaa
    31201 gaagcgggga tttccgtggc ggcgatgcgc catttcgccg gccggctgcc gattatgggc
    31261 gtgtgcctcg ggcatcagac gataggcgag gcgttcggcg gcaggatagt ccgcgccaaa
    31321 acgctgatgc acggcaaggt gtcgcccgtg tcccattcgg gcaagggtat gtttaaaggt
    31381 ttgcccaatc cggttacctg tacgcgttat cacagcctcg ttatcgaacg cggcacgttg
    31441 ccggattgct tggaaatcac ggcttggact gcggacggcg agattatggg cgtgcgccat
    31501 aaggaatatg ccgtcgaggg ggtacaattc caccctgaag ctctgctgac cgaacacgga
    31561 catgatatgt taaacaattt tttagttgaa tttcaaaact tcaaaccgca aaaaatctga
    31621 cgtgatgccg tctgaatccc ttcagacggc attttcgtcc gaatattgaa cggaggacaa
    31681 aaaatgatta caccgcaaca ggctatcgaa cgattaatca gcaataatga gttgttttac
    31741 gatgaaatga ccgacttgat gcgccaaatg atgagcggaa aagtgccgcc cgaacaaatt
    31801 gcggcgattt tgacaggatt gcgtatcaag gttgaaaccg tttccgaaat taccgcagct
    31861 gcagccgtca tgcgcgagtt tgcgacaaaa gtgccgctgg agaatgcgga ggggctggtt
    31921 gatatcgtcg gtacgggcgg ggatggtgcc aaaaccttca atatttcgac gacttcgatg
    31981 tttgttgccg cagcggcagg cgcgaaggtt gccaaacacg gcgggcgttc cgtttcatct
    32041 tccagcggcg cggcggacgt gatggagcag atgggcgcaa acctcaacct gactcccgaa
    32101 cagattgccc aaagtatcag ccagaccggt atagggttta tgttcgcccc caatcaccac
    32161 agtgccatgc gccatgtcgc cccggtacgc cgttcgctcg gtttccgaag cattttcaac
    32221 atattgggtc cgttgaccaa tcctgcgggc gcgccgaacc agcttttggg cgtgttccac
    32281 accgatttgt gcggcatttt gtcgcgggtc ttgcaacaac tcggttcaaa acacgtttta
    32341 gtcgtttgcg gggagggcgg tttggatgaa attacactga cgggcaaaac gcgcgttgcc
    32401 gaactcaaag acggaaaaat cagcgaatac gacatccgcc cagaagattt cggtatcgaa
    32461 acccgccgca atttggatga aatcaaagtt gccaatactc aggaatcttt gttgaaaatg
    32521 aatgaggtgc tggatgggaa agaaggggct gcgcgcgata tcgtattgct taataccgcc
    32581 gccgccctat atgccggaaa tgtcgctgct tcgctttcag acggcatatc tgccgcacgg
    32641 gaagccatcg attcaggcag ggccaaatcg aaaaaagagg agtttgtcgg ttttcaacca
    32701 caacaaagat gccattttct tggaaagatg gagcttgggt gatgccgcca tgattatgga
    32761 acttttgtgg caaaacataa gcacttcacg aagagaactt accaaactgt ttttatataa
    32821 aaacttgggg ctgtactaga taaccagacc aaattcccat taactaattg tcttaaaatc
    32881 tgaatttgag attctattta aaatgccatt ggcatttctt taaatgcagc cccaaatgct
    32941 ctttgggaat gccgttaaac ttacgtaaat ggctaaattc actaatatca agcacatcat
    33001 aactacgaaa ggtatccgtg tatacaatgc catcaggctt aactttcttt cggataattg
    33061 gcaacaatgt cgctgattgc gcattaggaa caacgacggt a
//
LOCUS       DAWFHY010000014        33020 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.014, whole genome shotgun sequence.
ACCESSION   DAWFHY010000014 DAWFHY010000000
VERSION     DAWFHY010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 33020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33020
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.014"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(304..1563)
                     /gene="rho"
                     /locus_tag="WGQ68_000555"
     CDS             complement(304..1563)
                     /gene="rho"
                     /locus_tag="WGQ68_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEZ0714414.1"
                     /translation="MHVSELQTLHISKLLELAEEHGIENANRFRKQDLVFAIVRQMMK
                     KGEGFTCSGTLEILPDGFGFLRSADTSYLAGPDDIYVSPTQIRRFNLHTGDTIEGSVR
                     VPKDNERYFALVRLDSINGDHPEVCRHKILFENLTPLFPTEQLKLERDLKSEENLTGR
                     AIDLISPIGKGQRALLVAPPKSGKTVMLQNIAHAVTSNYPEVELIVLLIDERPEEVTE
                     MSRSVRGEVVSSTFDEPAQRHVQVAEMVLEKAKRMVEHKKDVVILLDSITRLARAYNT
                     VVPTSGKILTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALVETGSRMDDVIYEE
                     FKGTGNMELHLDRRMAEKRLFPAININKSGTRREELLVPNDQLQRMWLLRKFLHPMDE
                     IEAAEFLIGKIKASKNNDDFFELMRGK"
     gene            complement(1783..4167)
                     /gene="ppsA"
                     /locus_tag="WGQ68_000556"
     CDS             complement(1783..4167)
                     /gene="ppsA"
                     /locus_tag="WGQ68_000556"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HEZ0714415.1"
                     /translation="MADNYVIWFENLRMTDVERVGGKNASLGEMISQLTEKGVRVPGG
                     FATTADAYRAFLAHNGLSERISAALAKLDVEDVAELARVGKEIRQWILDTPFPEQLDA
                     EIEAAWNKMVADAGGADISVAVRSSATAEDLPDASFAGQQETFLNINGLDNVKEAMHH
                     VFASLYNDRAISYRVHKGFEHDIVALSAGVQRMVRSDSGASGVMFTLDTESGYDQVVF
                     VTSSYGLGENVVQGAVNPDEFYVFKPTLKAGKPAILRKTMGSKHIKMIFTDKAEAGKS
                     VTNVDVPEEDRNRFSITDEEITELAHYALTIEKHYGRPMDIEWGRDGLDGKLYILQAR
                     PETVKSQEEGSRNLRRFSINGEKTVLCEGRAIGQKVGQGKVRLIKDASEMDSVEAGDV
                     LVTDMTDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATELLKNGQE
                     VTVSCAEGDTGFIYAGLLDVQITDVALDNMPKAPVKVMMNVGNPELAFSFANLPSEGI
                     GLARMEFIINRQIGIHPKALLEFDKQDDELKAEITHRIAGYASPIDFYVDKIAEGVAT
                     LAASVYPRKTIVRMSDFKSNEYANLVGGSIYEPHEENPMLGFRGAARYVADNFKDCFA
                     LECKALKRVRDEMGLTNVEIMIPFVRTLGEAEAVVKALKENGLERGKNGLRLIMMCEL
                     PSNAVLAEQFLQYFDGFSIGSNDMTQLTLGLDRDSGLVSESFDERNPAVKVMLHLAIS
                     ACRKQNKYVGICGQGPSDHPDFAKWLVEEGIESVSLNPDTVIETWLYLANELNK"
     gene            4578..5399
                     /locus_tag="WGQ68_000557"
     CDS             4578..5399
                     /locus_tag="WGQ68_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEZ0714416.1"
                     /translation="MSSPRHVFYISDRTGLTAENIGEALLNQFGNLSFKRHTHPFVDT
                     PEKARAVVEKVNRSRQENGQRPIAFVSVVDDEIRRIIKGADAFQINFFETFLGLLEKE
                     LNTEATASEQGHHSIGNTKRYDARMEAVNFSLNHDDGVSDKNLQEADVILMGVSRSGK
                     TPTCLYLALQYGIRAANYPLIPDDLESADLPRMVKPYRDKLFGLTIQPERLQAIRQER
                     RPNSTYAKIDTCRSEVADAQSMFRRHGIPFANTTDKSVEELAVHILQACKLKRRF"
     gene            complement(5639..6301)
                     /locus_tag="WGQ68_000558"
     CDS             complement(5639..6301)
                     /locus_tag="WGQ68_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0714417.1"
                     /translation="MIHAVLFDLDGTLADTALDLGGALNTLLARHGLPAKSMDEIRTQ
                     ASHGAAGLIKLGAGITPDHPDYARWRTEYLEEYDRRYAQDTELFDGVNELIAELNQRG
                     IKWGIITNKPMRFTDKLVPKLGFIIPPAVVVSGDTCGEPKPSIKPMLYACGQIHADPQ
                     HTLYVGDAERDIQAGRNAGMKTVLAEWGYISDEDDTDSWQADFHIRTPLDLLECLDKI
                     QP"
     gene            complement(6314..7141)
                     /locus_tag="WGQ68_000559"
     CDS             complement(6314..7141)
                     /locus_tag="WGQ68_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEZ0714418.1"
                     /translation="MKPDIYALLERALLSGNPDEKGRLADEAFAAVQNADGAETNAPP
                     ADFPRAGRPDKPVLVAPSQLTPRKMNTTEGYAAMLHAITHIEFNAVNLTLDAAYRFRT
                     LPFQFVRDWVKVAKEEVYHFRLMRDRLRTFGFDYGSFEAHNHLWDMAYKTAYDPLLRM
                     ALVPRVLEARGLDVTPGIRAKVAQRGDSATCGVLDIIYRDEVGHVAIGNHWYQHLCRE
                     RGLEPITLFRSLIARYDMFIFRGYVNIEAREKAGFSRFELDMLEDFEQGLKQNKNTV"
     gene            7419..8042
                     /gene="lolA"
                     /locus_tag="WGQ68_000560"
     CDS             7419..8042
                     /gene="lolA"
                     /locus_tag="WGQ68_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HEZ0714419.1"
                     /translation="MMKPHNLFQFLAVCSLTVAVASAQAGAVDALKQFNNDADGISGS
                     FTQTVQSKKKTQTAHGTFKILRPGLFKWEYTSPYKQTIVGDGQTVWLYDVDLAQVTKS
                     SQDQAIGGSPAAILSNKTALESSYTLKEDGSSNGIDYVLATPKRNNAGYQYIRIGFKG
                     GNLAAMQLKDSFGNQTSISFGGLNTNPQLSRGAFKFTPPKGVDVLSN"
     gene            8315..9454
                     /locus_tag="WGQ68_000561"
     CDS             8315..9454
                     /locus_tag="WGQ68_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0714420.1"
                     /translation="MKKTLVAAAILSLALTACGGGNDTAAQAPSAKPEAEQSGKLNIY
                     NWSDYVDPETVAAFEKETGIKTRSDYYDSNETLEAKVLTGKSGYDLTAPSIANVGRQI
                     KAGAYQKIDKAQIPHYGNIDKDLLKMMEAVDPGNEYAVPYFWGINTLAINTQQVKKAL
                     GTDKLPENEWDLVFKPEYTAKLKSCGISYFDSAIEQIPLALHYLGKDPNSENPEDIKA
                     AVDMMKAVRGDVKRFSSSGYIDDMAAGNLCAAIGYGGDLNIAKTRAEEAANGVEIKVL
                     TPKTGVGVWVDSFMIPRDAQNVANAHRYIDYTLRPEVAAKNGSFVTYAPASRPARDLM
                     DEKYTSDASIFPNKELMEKSFIVSPKSAESAKLGVKLWQGLKAGK"
     gene            9587..10643
                     /locus_tag="WGQ68_000562"
                     /pseudo
     CDS             9587..10643
                     /locus_tag="WGQ68_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            10684..11214
                     /locus_tag="WGQ68_000563"
     CDS             10684..11214
                     /locus_tag="WGQ68_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEZ0714421.1"
                     /translation="MNAIRTFQNRTPEIHESCMIDEACVVIGEVSLAEDVSVWPCAVL
                     RGDVNSITVGARSNIQDGSVLHVSHKTAAKPEGSPLVIGEDVTVGHKVMLHGCRIGNR
                     VLVGMGTTVLDDAVIEDEVMIGAGSLVPPRKRLEGGYLYVGSPVRQVRVLTDEEKAFL
                     KYSAAHYVKLSKQYGM"
     gene            complement(11231..11362)
                     /locus_tag="WGQ68_000564"
     CDS             complement(11231..11362)
                     /locus_tag="WGQ68_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor domain protein"
                     /protein_id="HEZ0714422.1"
                     /translation="MPSKPEKRASDGIFACNSNADGQNQGASDTAIGHPVRMNLGLT"
     gene            complement(11381..12976)
                     /locus_tag="WGQ68_000565"
     CDS             complement(11381..12976)
                     /locus_tag="WGQ68_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007342770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEZ0714423.1"
                     /translation="MSQEILDQVRRRRTFAIISHPDAGKTTLTEKLLLFSGAIQSAGT
                     VKGKKTGKFATSDWMEIEKQRGISVASSVMQFDYKDHTVNLLDTPGHQDFSEDTYRVL
                     TAVDSALMVIDAAKGVEAQTIKLLNVCRLRDTPIVTFMNKYDREVRDSLELLDEVENI
                     LQIRCAPVTWPIGMGKNFKGVYHILNDEIYLFEAGGERLPHEFDIIKGIENPELEQRF
                     PLEIQQLRDEIELVQAASNEFNLDEFLAGELTPVFFGSAINNFGIQEILNSLIEWAPA
                     PKPRDATVRMVEPDEPKFSGFIFKIQANMDPKHRDRIAFLRVCSGKFERGMKMKHLRI
                     NREIAASSVVTFMSHDRELVEEAYAGDIIGIPNHGNIQIGDSFSEGEQLAFTGIPFFA
                     PELFRSVRIKNPLKIKQLQKGLQQLGEEGAVQVFKPMSGADLILGAVGVLQFEVVTSR
                     LANEYGVEAVFDSASIWSARWVSCDDKKKLAEFEKANAGNLAIDAGGNLAYLAPNRVN
                     LGLTQERWPDIVFHETREHSVKL"
     gene            complement(13083..13478)
                     /gene="hisI"
                     /locus_tag="WGQ68_000566"
     CDS             complement(13083..13478)
                     /gene="hisI"
                     /locus_tag="WGQ68_000566"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HEZ0714424.1"
                     /translation="MDKNLLEAVKFDEKGLVCAIAQDAETKRVLMVAWMNAEALQKTV
                     ETGFAHYYSRSRQKQWMKGEESGHTQKVRELRLDCDGDAIVMLIAQNGGIACHTGRES
                     CFYKVWRGSAWETADAVLKDEKEIYGSTH"
     gene            complement(13509..14276)
                     /gene="hisF"
                     /locus_tag="WGQ68_000567"
     CDS             complement(13509..14276)
                     /gene="hisF"
                     /locus_tag="WGQ68_000567"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HEZ0714425.1"
                     /translation="MALAKRIIPCLDVKDGRVVKGVNFIGLRDAGNPVEAAKRYNGEG
                     ADELTFLDITASSDNRDTILHIIEEVAGQVFIPLTVGGGVRTVADIRRLLNAGADKVS
                     INTAAVTRPDLIDEAAGFFGSQAIVAAVDAKAANPENTRWEIFTHGGRNPTGLDAVEW
                     AIEMQKRGAGEILLTGMDRDGTKQGFNLPLTRAVAEAVDIPVIASGGVGNVRHLIEGI
                     TEGKADAVLAAGIFHFGEIAIREAKRAMREAGIEVRL"
     gene            complement(14289..15026)
                     /gene="hisA"
                     /locus_tag="WGQ68_000568"
     CDS             complement(14289..15026)
                     /gene="hisA"
                     /locus_tag="WGQ68_000568"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HEZ0714426.1"
                     /translation="MLLIPAIDLKEGRCVRLKQGLMEEATVFSDSPAETALHWFKQGA
                     RRLHLVDLNGAFAGVPQNLPAIKDILAAVARDIPVQLGGGIRDLKTIGQYLDLGLNDV
                     IIGTAAVKNPDFVREACKAFPGRIIVGLDAKDGMAAIDGWATVTGHHVIDLAKRFEDD
                     GVNSIIYTDIGRDGMMSGVNIDATVKLAQAVRIPVIASGGLTGLDDIRALCAAEKHGV
                     AGAITGRAIYEGSIDFAQAQQLADSLD"
     gene            complement(15059..15697)
                     /gene="hisH"
                     /locus_tag="WGQ68_000569"
     CDS             complement(15059..15697)
                     /gene="hisH"
                     /locus_tag="WGQ68_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HEZ0714427.1"
                     /translation="MQIAIIDYGMGNLHSVLKSVRTAGQLAGKNTEIFLSGDPDRVSR
                     ADKVIFPGQGAMPDCMAALTRDGLDEAVKDALKNKPFFGICVGAQLLFDHSEEGNTDG
                     LGWFGGKVRRFARDLRDRQGCRLKVPHMGWNTVRQTQNHPLFQGIPQDTRFYFVHSYY
                     FAPENPETILGESDYPSPFACIVGKDNVFATQFHTEKSHDAGLTMLKNFLNW"
     gene            complement(15828..17330)
                     /gene="pta"
                     /locus_tag="WGQ68_000570"
     CDS             complement(15828..17330)
                     /gene="pta"
                     /locus_tag="WGQ68_000570"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEZ0714428.1"
                     /translation="MAKVLIVPVSAGLDASAAAQAFAKALDAQIFQAVDATAETLLAQ
                     GKSDDWFDALVGKVAALDAANLVIEGIAPDADKIYLAGKNVELALSLDAAAVFAVRSD
                     NADADELANRLNLAKQFFAAAPGVLEGFVVDGAAASVAEAAAEKTGLTFFGSSDALKD
                     VSVLAGREAKRLSPAQFRYNLIDFARQADKRIVLPEGAEPRTVQAAAICHEKGIARCV
                     LLAKREEVEAVAKERGISLPDSLEIIDPASLVEQYVEPMCELRKSKGLTPEDARKQLQ
                     DTVVLGTMMMAQNDVDGLVSGAVHTTANTIRPALQLIKTAPGASLVSSVFFMLLPNQV
                     LVFGDCAVNPNPTAQQLADIAIQSADSAKAFGIDPKVAMISYSTVNSGSGPDVDTVIE
                     ATKLAREKRPDLAIDGPLQYDAATVPGVGKSKAPGSQVAGQATVLVFPDLNTGNCTYK
                     AVQRSANVLSVGPLLQGLRKPVNDLSRGALVEDIVFTIALTAVQAKQMEG"
     gene            complement(17515..18573)
                     /gene="fbpC"
                     /locus_tag="WGQ68_000571"
     CDS             complement(17515..18573)
                     /gene="fbpC"
                     /locus_tag="WGQ68_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein FbpC"
                     /protein_id="HEZ0714429.1"
                     /translation="MTAALHIGHLSKSFQNTPVLNDISLSLDPGEILFIVGASGCGKT
                     TLLRCLAGFEQPDFGEISLSGRTIFSKNTNLPVRERRLGYVVQEGVLFPHLTVYRNTA
                     YGLGNGKGKTAQERQRIEAMLELTGISELAGRYPHELSGGQQQRVALARALAPDPELI
                     LLDEPFSALDEQLRRQIREDMIAALRANGKSAVFVSHDREEALQYADRIAVMKQGRIL
                     QTASPHELYRQPADLDAALFIGEGIVFPAALNADGTADCGLGRLPVQSGAPAGTRGTL
                     LIRPEQFSLHPHSAPTASIHAVVLKTTPKARHTEISLRVGQTVLTLNLPSAPTLSDGI
                     SAVLHLDGPALFFPGNTL"
     gene            complement(18594..20111)
                     /gene="fbpB"
                     /locus_tag="WGQ68_000572"
     CDS             complement(18594..20111)
                     /gene="fbpB"
                     /locus_tag="WGQ68_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease FbpB"
                     /protein_id="HEZ0714430.1"
                     /translation="MSPKKIPIWLTGLILLIALPLTLPFLYVAMRSWQVGINRAVELL
                     FRPRMWDLLSNTLTMMAGVTLISIVLGIACALLFQRYRFFGKTFFQTAITLPLCIPAF
                     VSCFTWISLTFRVEGFWGTVMIMSLSSFPLAYLPVEAALKRISLSYEEVSLSLGKSRL
                     QTFFSAILPQLKPAIGSSVLLIALHMLVEFGAVSILNYPTFTTAIFQEYEMSYNNNTA
                     ALLSAVLMAVCGIVVFGESIFRGKAKIYHSGKGVARPYPVKTLKLPGQIGAIVFLSSL
                     LTLGIIIPFGVLIHWMMVGTSGTFALVSVFDAFIRSLSVSALGAILTILCALPLVWAS
                     VRYRNFLTVWIDRLPFLLHAVPGLVIALSLVYFSINYTPAVYQTFIVVILAYFMLYLP
                     MAQTTLRTSLEQLPKGMEQVGATLGRGHFFIFRTLVLPSILPGITAAFALVFLKLMKE
                     LTATLLLTTDDVHTLSTAVWEYTSDAQYAAATPYALMLVLFSGIPVFLLKKYAFK"
     gene            complement(20179..21174)
                     /gene="fbpA"
                     /locus_tag="WGQ68_000573"
     CDS             complement(20179..21174)
                     /gene="fbpA"
                     /locus_tag="WGQ68_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     FbpA"
                     /protein_id="HEZ0714431.1"
                     /translation="MKTSIRYALLAAALTAATPALADITVYNGQHKEAAQAVADAFTR
                     ATGIKVKLNSAKGDQLAGQIKEEGSRSPADVFYSEQIPALATLSAANLLEPLPASTIN
                     ETRGKGVPVAAKKDWVALSGRSRVVVYDTRKLSEKDLEKSVLNYATPKWKNRIGYAPT
                     SGAFLEQVVAIVKLKGEAAALKWLKGLKEYGKPYAKNSVALQAVENGEIDAALINNYY
                     WHAFAREKGVQNVHTRLNFVRHRDPGALVTYSGAAVLKSSQNKDEAKKFVAFLASKEG
                     QRALTAVRAEYPLNPHVVSTFNLEPIAKLEAPQVSATTVSEKEHATRLLEQAGMK"
     gene            complement(21425..21894)
                     /locus_tag="WGQ68_000574"
                     /pseudo
     CDS             complement(21425..21894)
                     /locus_tag="WGQ68_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4375 domain-containing protein"
     gene            complement(21949..23325)
                     /gene="argH"
                     /locus_tag="WGQ68_000575"
     CDS             complement(21949..23325)
                     /gene="argH"
                     /locus_tag="WGQ68_000575"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEZ0714432.1"
                     /translation="MHDKTWSGRFNEPVSELVKQYTASIGFDRRLAEWDIQGSLAHAQ
                     MLKETGVLDDGDLADIRRGMAEILEEIRSGKIEWSSDLEDVHMNIERRLTDKIGDAGK
                     RLHTGRSRNDQVATDIRLWLRDQITVIQNLIQNLQTALLDLAEQNAETVMPGFTHLQV
                     AQPVSFGHHMLAYVEMLGRDNERMADCRRRVNRMPLGAAALAGTTYPIRREITAELLG
                     FEQICQNSLDAVSDRDFAVEFTAAASLVMVHLSRLSEELILWMSPRFGFIDIADRFCT
                     GSSIMPQKKNPDVPELVRGKSGRVIGHLIGLITLMKSQPLAYNKDNQEDKEPLFDTAD
                     TLIDTLRIYADMMRGVTVKPDNMRAAVMQGFATATDLADYLVKKGMPFRDAHEVVAQA
                     VRHADQAGVDLSELPLEVLQGFSDLIADDVYGVLTPEGSLNARNHLGGTAPEQVRFQV
                     KRWREVLA"
     gene            complement(23344..24213)
                     /gene="galU"
                     /locus_tag="WGQ68_000576"
     CDS             complement(23344..24213)
                     /gene="galU"
                     /locus_tag="WGQ68_000576"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEZ0714433.1"
                     /translation="MKPIRKAVFPVAGMGTRFLPATKASPKEMLPIVDKPLIQYAVEE
                     AVEAGCTEMVFVTGRNKRSIEDHFDKAYELETELEMRHKDKLLEHVRNILPPNITCLY
                     IRQAEALGLGHAVLCARAAIGDEPFAVILADDLIDAPKGALKQMVEVYGRSGNSILGV
                     ETVEASQTGSYGIVETEQLKQFQRIIGIVEKPKPEDAPSNLAVVGRYILTPRIFDLLT
                     GLPRGAGNEIQLTDGIAKLLDHEFVLAHPFEGTRYDCGSKLGYLEATVAYGLKHPETG
                     EPFRRLLEKYRTE"
     gene            complement(24241..24840)
                     /gene="rdgB"
                     /locus_tag="WGQ68_000577"
     CDS             complement(24241..24840)
                     /gene="rdgB"
                     /locus_tag="WGQ68_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEZ0714434.1"
                     /translation="MSEKPEKIVLASGNAGKLKEFGNLFEPYGITVLPQSEFSIPECP
                     EPYSTFVENALAKARHAAGHSGLPALADDSGICAAALNGAPGIHSARYAGSNPKSDTA
                     NNLKLAAELAGKADKSCCYVCVLVFVRHKDDPRPIIAEGVWHGQWNGVPAGENGFGYD
                     PYFYLPGHGKTAAELDSEVKNRESHRAQALAELLRKLAL"
     gene            complement(24833..25066)
                     /locus_tag="WGQ68_000578"
     CDS             complement(24833..25066)
                     /locus_tag="WGQ68_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714435.1"
                     /translation="MNRRKIYLLSVALFTLAFMLLVLLGAYLLTVGSKAFAVASFLFA
                     FGALFGQIGSLALYLRHKSLRAAQSAAKENRYV"
     gene            complement(25174..25707)
                     /locus_tag="WGQ68_000579"
     CDS             complement(25174..25707)
                     /locus_tag="WGQ68_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HEZ0714436.1"
                     /translation="MADFNQILTPGDVDGGIINVVNEIPAGSNHKIEWNRKLAAFQLD
                     RVEPAIFAKPTNYGFIPQTLDEDGDELDVLLVTEQPLATGVFLEARVIGVMKFVDDGE
                     VDDKIVCVPADDRNNGNAYKTLSDLPQQLIKQIEFHFNHYKDLKKAGTTKVESWGDAE
                     EAKKVIKESIERWNKQA"
     gene            complement(25808..26266)
                     /gene="nudB"
                     /locus_tag="WGQ68_000580"
     CDS             complement(25808..26266)
                     /gene="nudB"
                     /locus_tag="WGQ68_000580"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HEZ0714437.1"
                     /translation="MPMAKPLKYPVSALVVLYSGDGGILLIERTHPEGFWQSVTGSLE
                     PGETVAQTARREVWEETGILLADGQLQDWHDSTVYEIYHHWRHRYPKGVFENREHLFS
                     AEIPRDTPIALQPEEHVSYGWFDMEEAAEKVFSPSNRRAILELGRFLGKR"
     gene            26477..26553
                     /locus_tag="WGQ68_000581"
     tRNA            26477..26553
                     /locus_tag="WGQ68_000581"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26511..26513,aa:Pro,seq:ggg)
     gene            26711..28537
                     /gene="mafB"
                     /locus_tag="WGQ68_000582"
     CDS             26711..28537
                     /gene="mafB"
                     /locus_tag="WGQ68_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 2"
                     /protein_id="HEZ0714438.1"
                     /translation="MGISRKISLILSILAVCLPMHAHASDLANDSFIRQVLDRQHFEP
                     DGKYHLFGSRGELAERSGHIGLGKIQSHQLGNLMIQQAAIKGNIGYIVRFSDHGHEVH
                     SPFDNHASHSDSDEAGSPVDGFSLYRIHWDGYEHHPADGYDGPQGGGYPAPKGARDIY
                     SYDIKGVAQNIRLNLTDNRSTGQRLADRFHNAGSMLTQGVGDGFKRATRYSPELDRSG
                     NAAEAFNGTADIVKNIIGAAGEIVGAGDAVQGISEGSNIAVMHGLGLLSTENKMARIN
                     DLADMAQLKDYAAAAIRDWAVQNPNAAQGIEAVSNIFTAVIPVKGIGAVRGKYGLGGI
                     TAHPVKRSQMGAIALPKGKSAVSDNFADAAYAKYPSPYHSRNIRSNLEQRYGKENITS
                     STVPPSNGKNVKLADQRHPKTGVPFDGKGFPNFEKHVKYDTKLDIQELSGGGIPKAKP
                     VFDAKPRWEVDRKLNKLTTREQVEKNVQEIRNGNKNSNFSQHAQLEREINKLKSADEI
                     NFADGMGKFTDSMNDKAFSRLVKSVKENGFTNPVVEYVEINGKAYIVRGNNRVFAAEY
                     LGRIHELKFKKVDFPVPNTSWKNPTDVLNESGNVKRPRYRSK"
     gene            28540..29004
                     /locus_tag="WGQ68_000583"
     CDS             28540..29004
                     /locus_tag="WGQ68_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714439.1"
                     /translation="MAIWNYRYYLIPSAAIRNKFNANKDIILDEYRSNGFQNFNENKS
                     FENYFIDNDVILLSIINEAKKQLKLKESWDKDAIMFCDNFGNSLTVWPDDIECELDLR
                     FDYTKFIQKTIDWAIKYNCLLVIEKTGNVVSPNINNLMYEIKAYLESKPWPI"
     gene            29193..29375
                     /locus_tag="WGQ68_000584"
                     /pseudo
     CDS             29193..29375
                     /locus_tag="WGQ68_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223961.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
     gene            29387..29674
                     /locus_tag="WGQ68_000585"
     CDS             29387..29674
                     /locus_tag="WGQ68_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HEZ0714440.1"
                     /translation="MQLEIIGSKIYTEQDFHNQISKIFSIQDYYGNNLDALWDLLSTN
                     VERPITLVWKDAMFSKNQLENIFIEIVNVLERVKKQDEDYGFEEKFNYILE"
     gene            30170..31432
                     /locus_tag="WGQ68_000586"
     CDS             30170..31432
                     /locus_tag="WGQ68_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0714441.1"
                     /translation="MKSKSGVIASLTGAAVGGTPVDAQTGGAVGQNAVENNLYLTSEA
                     LKKDKQTARKIYSVIKEQVKHECSSTGRITECRQNIGRIIEFTQDKRFDSRFKDLKKE
                     SLYYLNKHPDLVASYLKAEYEKLDREDKSILHRYISPGAEIVSGSLGVVLSGVAGGGS
                     CAETFGLGCAAALVGVTSSYDHVITGTKNFGKKASEQRPTIAVQALKQLGLSEQAAEY
                     VQFSIDLFSVGKSGGGIPKAKPVFDAKPRWEVDRKLNKLTTREQVEKNVQETRRRSQS
                     SQFKAHAQREWENKTGLDFNHFIGGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGV
                     YQATVEIKKPDGSWEMKKGRGGKPMTNHSMFPKDWDEARIRAEVTSAWEKRKELEGQK
                     WRGTSKSGVEIEGFTEPNRTAYPLYNSK"
     gene            31459..31878
                     /locus_tag="WGQ68_000587"
     CDS             31459..31878
                     /locus_tag="WGQ68_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714442.1"
                     /translation="MYKIKCFYDQFSSGELFNYCQFLDNSSNQKINTRGTVYQYIIYV
                     LTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYHNEFDEEDGW
                     PDLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            31956..32039
                     /locus_tag="WGQ68_000588"
                     /pseudo
     CDS             31956..32039
                     /locus_tag="WGQ68_000588"
                     /inference="COORDINATES: protein motif:HMM:NF026862.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            32036..32278
                     /locus_tag="WGQ68_000589"
     CDS             32036..32278
                     /locus_tag="WGQ68_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein 31"
                     /protein_id="HEZ0714443.1"
                     /translation="MTNLKLDFYSEVIIKDSCPNDLLENGETIKGKKGVVLGISEEDG
                     IIYGYTILLFDIKYCIYIDKKYIIPTGKKFSRDDFY"
     gene            32356..32601
                     /locus_tag="WGQ68_000590"
     CDS             32356..32601
                     /locus_tag="WGQ68_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0714444.1"
                     /translation="MKDSTGQMRLATKDLAEAIKRGEVRSSAFTTKQLKAIEKGESKI
                     PDYTWHHHQDTGRMQLINEDLHHDTGHIGWRAMSKGK"
     gene            32604..32994
                     /locus_tag="WGQ68_000591"
                     /pseudo
     CDS             32604..32994
                     /locus_tag="WGQ68_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7813 a         7713 c         9340 g         8154 t
ORIGIN      
        1 aggcaactct aacttaattt gaatttccct agttatctag gacagcccca aatttataca
       61 aaaatgagcg cggttcggcg caaccttgaa tcaagttccc gcatcggttt tcattgccgg
      121 tacggatgcg ttcaagccgg ttttgcaaag gccgcgcttt cggcaagcgg acacggacac
      181 tgccggcggt tgcgccgtta acggggggag ggaggagctg cgccggccgt gtgaatgaaa
      241 gtgccgtctg aaacccgatt ttcaggcttc agacggcatt tcgcattaat gcgggtggcg
      301 cgtttatttg ccgcgcatca gttcaaagaa atcgtcgttg tttttagagg ctttgatttt
      361 cccgattaaa aattcggctg cctcgatttc gtccatcggg tgcaggaact tgcgtaagag
      421 ccacatacgt tgtaactggt cgttcggcac aagcagctct tcgcggcgcg tgccggattt
      481 gttgatgttg atggcgggga agaggcgttt ttccgccata cggcggtcaa ggtgcaattc
      541 catattgccg gtgcctttga attcttcgta aatcacatcg tccatacggc tgccggtttc
      601 aaccaatgcg gtggcgatga tggtcagcga accgccttct tccacgttgc gcgccgcgcc
      661 gaagaaacgt ttggggcggt gcagtgcgtt ggcatcgaca ccgccggtta ggattttgcc
      721 cgaggtaggc acgacggtat tgtaggcgcg ggcaaggcgg gtaatcgaat ccagcaggat
      781 gaccacgtct tttttgtgtt ctaccatacg cttggctttt tcaagcacca tttcggcaac
      841 ttggacgtgg cgttgcgccg gttcgtcaaa ggtggaggaa accacttcgc cgcggacgga
      901 acggctcatt tcggttactt cttcggggcg ttcgtcaatc aagaggacga tgagttcgac
      961 ttcgggatag tttgaggtaa cggcgtgggc aatgttttgc agcatcacgg ttttaccgct
     1021 tttgggcggg gcaaccaaga gggcgcgctg acctttgccg atgggcgaaa tcaggtcgat
     1081 ggctcgtccg gtcaggtttt cttcggactt taagtcgcgt tccagcttca actgttcggt
     1141 cggaaacagc ggggtcaggt tttcaaacag gattttatgg cggcatactt ccgggtggtc
     1201 gccgttgatg gaatccagtc tgactagggc aaaatagcgt tcgttgtctt tggggacgcg
     1261 cacgctgcct tcgatggtgt cgcccgtatg caggttgaag cggcggattt gggtgggcga
     1321 gacatagatg tcgtcggggc ccgcaagata ggacgtgtcc gcgctgcgga ggaagccgaa
     1381 gccgtcgggc aggatttcga gcgtgccgga gcaggtgaaa ccctcgcctt ttttcatcat
     1441 ttggcggacg atggcaaata cgaggtcttg tttgcggaat cggttggcgt tttcgatgcc
     1501 gtgttcttcc gccaattcta agagtttgga aatgtgcagg gtttgtaatt cggagacgtg
     1561 cataataatg atgtattttg aagaggaaaa agacaggcag atgccgtctg aaagaagaag
     1621 ctgaccgttg gcggttgctc ggggaagggg gaattgtagg cagtcggcgc gtgggtgtca
     1681 aatattatcg cggacggggc atcggcagga aatgccgtct gagcggagct gcttggaaaa
     1741 aaataccccc gcgcttttca ggctcggggg tatgggcatt gattatttgt tcaattcatt
     1801 cgccaaatat agccaagttt cgatgacggt atccgggttc agggaaacgc tttcaatgcc
     1861 ttcctcaacc agccatttgg cgaagtccgg atggtcggac gggccttgac cgcagatgcc
     1921 gacatatttg ttctgcttgc ggcaggcgga gatggcaagg tgcagcatca ctttgacggc
     1981 agggttgcgt tcgtcaaacg attcggatac caagccgctg tcgcggtcga gaccgagggt
     2041 cagttgggtc atgtcgttcg agccgatgga gaagccgtcg aagtattgca ggaattgttc
     2101 cgccaatacc gcgttgctcg gcagctcgca catcataatc aggcgcaggc cgtttttgcc
     2161 gcgttccaag ccgttttctt tcagggcttt gacaacggct tcggcttcgc ccaaagtgcg
     2221 gacgaacgga atcatgattt caacgttggt caaccccatt tcatcgcgga cgcgtttcaa
     2281 ggctttgcat tccaaggcga aacagtcttt gaagttgtcg gcgacataac gcgccgcgcc
     2341 ccggaagccc aacatcgggt tttcttcgtg cggttcgtag atgctgccac ccaccaagtt
     2401 agcgtactcg ttggatttga agtcggacat acggacgatg gttttacgcg gataaaccga
     2461 tgcggctaaa gttgccacgc cttctgcgat tttatcgaca tagaagtcaa taggagaggc
     2521 gtagccggcg atgcggtggg taatttcagc ttttaactcg tcgtcttgtt tgtcaaattc
     2581 caacaaggct ttggggtgga tgccgatttg gcggttgatg atgaattcca tacgcgccaa
     2641 gccgatgcct tcgctcggca ggttggcgaa gctgaatgcg agttcgggat tgccgacgtt
     2701 catcatgact tttacaggtg ctttaggcat attgtccaag gcgacatcgg taatctgtac
     2761 gtccaacagg ccggcataga tgaagccggt atcgccttcg gcgcaggata cggtaacttc
     2821 ctgaccgttt ttcagcaatt cggttgcatt gccgcagccg acgacggcag gaatgcccaa
     2881 ttcacgcgcg ataatcgctg cgtggcaggt acgtccgccg cgattggtaa cgatggcgga
     2941 agcgcgtttc atcaccggtt cccaatccgg atcggtcata tcggtaacca gtacgtcgcc
     3001 ggcttcgacg gaatccatct cggaagcatc tttaatcagg cgtactttgc cctgtccgac
     3061 tttctgaccg atggcgcggc cttcgcacaa gacggttttt tcgccgttga tagaaaagcg
     3121 gcgcaggttg cggctgcctt cttcttggga tttcacggtt tcgggacggg cttgcaggat
     3181 atagagtttg ccgtccaagc cgtcgcgacc ccattcgata tccatcgggc ggccgtagtg
     3241 tttttcgatg gtcagcgcgt aatgcgccaa ctcggtgatt tcttcgtcgg taatggagaa
     3301 gcggttgcgg tcttcttcgg ggacatcgac gttggttacg gatttaccgg cttctgcttt
     3361 gtcggtaaaa atcattttga tgtgtttcga acccatggtt ttacgcagga tggcgggctt
     3421 gcccgctttg agcgtaggtt tgaacacata aaattcgtcc gggttgaccg cgccttgtac
     3481 gacgttttcg cccagaccgt aagaggaggt aacaaagacg acttgatcgt agccggattc
     3541 ggtgtcgagg gtgaacatca cacctgatgc gccgctgtcg gaacgcacca tacgctgcac
     3601 gccggcggaa agggcgacga tgtcgtgttc gaagcctttg tggacgcggt aagaaatggc
     3661 acggtcgtta tacagggagg cgaatacatg gtgcatcgct tctttaacgt tatccaagcc
     3721 gttgatgttc aagaaggttt cctgttgtcc agcgaatgat gcgtccggca ggtcttcggc
     3781 ggttgcggaa gaacgtacgg caacggaaat gtccgcaccg ccggcatcgg caaccatttt
     3841 gttccatgcc gcttcgattt cggcatcgag ctgttcgggg aaaggcgtgt ccaaaatcca
     3901 ttggcggatt tctttgccga cgcgtgccag ttcggcaacg tcttcgacat ccaattttgc
     3961 cagtgcggcg gaaatgcgtt cgctcagacc gttgtgtgcg aggaatgcgc ggtaggcatc
     4021 ggctgtggtg gcaaagccgc cggggacgcg aacgcctttt tcggtcagct gactgatcat
     4081 ttcgcccagc gaggcgtttt taccgcccac gcgttcaaca tctgtcatac gcaggttttc
     4141 aaaccagatt acgtagttgt cggccatttg tgtgtccaat ccaaaatatg ttaaaaaaga
     4201 aacaaatccg cgtgcttatt ttaagcgatt cgttcctctg ctgtcatgtg ttttatccat
     4261 tttaaaatca tgatgccgtc tgaaaaattg cggtttcggc gtgtgtagcg gtttgaaact
     4321 tgcagccggt atacttcttt ttttgggtat tttctttgta aaacaggtgg tttgaatagg
     4381 ttaatgtttt ttctgtttga tttttttgtt tattttttaa aattttctgc caaaaaatac
     4441 tttatataaa taattttatt tcaaaattat attgtgtctg tttgggtgta atccgaggta
     4501 ggtgtgctgc gagtgctttc cttgtgtctg ccgctgctgt tatgatgata tttttattct
     4561 gtatttaagg atggaagatg agcagtccgc gccatgtgtt ttacatttcc gaccgtaccg
     4621 gtctgactgc cgagaatatc ggcgaggctt tgctgaacca gtttggcaat ctgtcgttca
     4681 aacgccatac gcatccgttt gtcgatacgc cggaaaaggc gcgcgcggtg gtggagaagg
     4741 tcaatcggag ccggcaggaa aacggtcagc gtccgattgc gtttgtcagt gtggttgatg
     4801 acgaaatccg tcggattatc aaaggggcgg atgcttttca gattaatttc tttgagactt
     4861 ttttgggact gttggagaaa gaactcaata ccgaagccac ggcatccgag caggggcatc
     4921 acagtatcgg taatacgaag cgttacgatg cgcgtatgga ggcggtcaat ttttctttga
     4981 accacgatga cggggtcagc gataagaacc ttcaggaagc ggatgtaatc ttgatgggtg
     5041 tatcgcgttc gggcaaaacg ccgacctgcc tttacctcgc cctgcaatac ggcatccgtg
     5101 cggcaaacta tccgctgatt cccgacgatt tggaatcggc cgatctgccg cgtatggtca
     5161 agccttatag ggataagctg ttcgggttga ccatccagcc ggaacgtttg caggccatcc
     5221 gccaagagcg ccgcccgaat tcgacttatg ccaaaatcga tacatgccgc agcgaggtgg
     5281 cagacgcgca gagtatgttc agacggcatg ggattccgtt tgcgaatacg acggataaat
     5341 cagtggaaga gcttgccgta cacatccttc aggcgtgcaa gctcaaacgc aggttttgac
     5401 gggttttgat tcggtttgaa ggtggaaatg ccgtctgaaa tcaggtttca gacggcagtt
     5461 ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt acaaatagta
     5521 cggagccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg agctaaggcg
     5581 aggcaacgct gtgctggttt agatttaatc cactatatgt ttgtggggcg gatatttttc
     5641 agggctgtat tttgtccaga cattcgagca gatcgagcgg cgttcggatg tggaaatccg
     5701 cctgccatga gtcggtatcg tcttcatctg agatgtagcc ccattcggcg aggacggttt
     5761 tcatgccggc gttgcggcct gcttggatgt cgcgttcggc atcaccgacg tagagtgtgt
     5821 gttgcgggtc ggcgtggatt tgtccgcacg catacagcat gggtttgatg ctgggcttgg
     5881 gttcgccgca ggtgtcgccg ctgacgacga cggcgggtgg gatgatgaag ccgagtttgg
     5941 ggacgagttt gtcggtgaag cgcatgggtt tgttggtgat gatgccccat ttgatgccgc
     6001 gttggttgag ttcggcaatg agttcgttca cgccgtcaaa gagctcggtg tcttgggcgt
     6061 agcggcggtc gtattcctcc agatattcgg tgcgccatcg ggcatagtcg gggtggtcgg
     6121 gggtgatgcc tgcgccgagt ttgatcagtc ctgccgcgcc gtggctggct tgggtgcgga
     6181 tctcgtccat gctttttgca ggtagtccgt ggcgagcgag cagggtgttg agtgcgcctc
     6241 cgaggtcgag ggcggtgtcg gcaagtgtgc cgtcgaggtc gaagagtacg gcgtgtatca
     6301 tgtgtgttcc ttttcagacg gtatttttgt tctgtttcaa accctgctcg aaatcttcca
     6361 gcatatccaa ttcaaagcgg ctgaagcccg ctttttcgcg cgcttcgatg ttcacatagc
     6421 cgcggaagat aaacatatcg taacgggcaa tcagactgcg gaacagggta atcggttcca
     6481 aaccgcgttc gcggcagagg tgttgatacc aatggttgcc gatggcgaca tgacccactt
     6541 cgtcgcggta aatgatgtcc aacacgccgc aggttgccga atcgccgcgc tgcgccacct
     6601 tcgcgcgtat ccccggcgta acgtccagcc cgcgcgcttc caacacgcgc ggcaccaaag
     6661 ccatacgcaa cagtgggtcg taggcggttt tgtacgccat atcccacaaa tggttgtgcg
     6721 cctcgaaact gccgtaatcg aagccgaaag tgcgcaatct gtcgcgcatc aggcggaaat
     6781 ggtacacctc ttccttcgcc actttcaccc agtcgcggac aaactgaaac ggcagcgtgc
     6841 ggaaacggta tgccgcgtcc aaagttagat tgacggcgtt gaactcgata tgcgtaatcg
     6901 cgtgcagcat cgccgcataa ccttcggttg tgttcatttt gcgcggggtc agttgcgacg
     6961 gcgcgaccaa aacaggcttg tctggccgtc ccgctcgggg gaagtccgcc ggcggcgcgt
     7021 ttgtttccgc cccgtccgca ttttgaacgg cggcaaacgc ctcatccgcc agccgtcctt
     7081 tttcatcggg gttgcccgaa agcagggcgc gttccagcaa agcataaata tcgggtttca
     7141 tttcaagtcc gccgtgttcg gaaaacaaat attatagcgt ttaaaaaaaa caagatgagg
     7201 catataatct ccgcgattcg gcattccgcg cccaaaccgt caaatcacgc ggcacacaat
     7261 taaagggcag cgtggcgcgc ctaccttttc cgagcggtca aaaaaatcag ccctcggaaa
     7321 acgcggtttg caaaatgcaa accgcccgta acgccgcccg tatgattgtt ttgctgcgcc
     7381 gatactttac gccacaccca tcccaacaag gaaaaataat gatgaaaccg cacaacctgt
     7441 tccaatttct cgccgtttgc tccctgaccg tcgccgtcgc ttccgcacag gcgggcgcgg
     7501 tagacgcgct caagcaattc aacaacgatg ccgacggtat cagcggcagc ttcacccaaa
     7561 ccgtccaaag caaaaagaaa acccaaaccg cgcacggcac gttcaaaatc ctgcgcccgg
     7621 gcctcttcaa atgggaatac acttcgccct acaaacagac tattgtcggc gacggtcaaa
     7681 ccgtttggct ctacgatgtt gatttggcac aagtgaccaa gtcgtcccaa gaccaggcca
     7741 taggcggcag ccccgccgcc atcctgtcga acaaaaccgc cctcgaaagc agctacacgc
     7801 tgaaagagga cggttcgtcc aacggcatcg attatgtgct ggcaacgccc aaacgcaaca
     7861 acgccggcta ccaatacatc cgcatcggct tcaaaggcgg caacctcgcc gccatgcagc
     7921 ttaaagacag cttcggcaac caaacctcca tcagtttcgg cggtttgaat accaatcccc
     7981 aactctcgcg cggcgcgttc aagtttaccc cgcccaaagg cgtggacgtg ttgagcaact
     8041 gatgccgtcc gccccgatgc cgtctgaaag ccgccgaggc ttcagacggc attttcacgc
     8101 aggcggaaca atgtcccgca ttaccgcccg ttcgggcatc ggaacggcaa acccgtgaaa
     8161 attaacggtt gcgccccggt tgtttttgcc gtttaatgca aaccttgctg caccaagggc
     8221 caagaaagcc gaccggccgc ccccacagct tccgatgcag gcggcccggc cgtccctgca
     8281 atgtttttta tttttgaacg aaaggtcgaa aaccatgaaa aaaacactgg tggcggcggc
     8341 aatcctgagc ctcgccttga ctgcgtgcgg cggcggaaac gataccgccg cccaagcccc
     8401 ctccgccaag cccgaagccg aacaatcggg caaactcaat atctacaact ggtcggatta
     8461 tgtcgatccc gaaaccgtcg ccgcctttga aaaagaaacc ggcatcaaga cgcgttccga
     8521 ttattacgac agcaacgaaa cactggaggc aaaagtcctg accggcaaat ccggctacga
     8581 cctgaccgcg ccgtccatcg ccaacgtcgg ccggcaaatc aaagcgggcg cgtatcagaa
     8641 aatcgacaag gcgcaaatcc cccattacgg caacatcgat aaagatttgc tgaaaatgat
     8701 ggaagccgtc gatccgggca acgaatacgc cgtcccctat ttctggggca tcaatacctt
     8761 ggcaatcaat acccagcagg tgaaaaaagc attgggtacg gacaagctgc ccgaaaacga
     8821 atgggatttg gtgttcaaac ccgaatacac cgccaaactc aaatcctgcg gcatcagcta
     8881 tttcgacagc gcaatcgaac agattccctt ggcgttgcac tatttgggca aagaccccaa
     8941 cagtgagaat cccgaagaca tcaaagccgc cgtcgatatg atgaaagccg tccggggcga
     9001 cgtgaaacgc ttcagctctt ccggctatat cgacgatatg gcggcgggca acctgtgtgc
     9061 cgccatcggt tacggcggcg atttgaacat tgccaaaacc cgtgccgaag aagccgcaaa
     9121 cggcgtggaa atcaaagtat tgaccccgaa aaccggcgtg ggcgtgtggg tggattcctt
     9181 tatgattccg cgcgacgcgc aaaacgttgc caacgcccac cgctatatcg actacacgct
     9241 ccggcccgag gtggcggcga aaaacggcag cttcgttacc tacgcgcccg ccagccgccc
     9301 cgcgcgcgat ttgatggatg aaaaatacac ctccgacgca tcgattttcc cgaacaaaga
     9361 actgatggaa aaaagtttca tcgtatcgcc caaatccgca gaatccgcca aactgggcgt
     9421 gaagctgtgg caagggctca aggccggaaa ataaccgcaa cccctgccgt ctgaaacctt
     9481 tcgggcggca ggaaacggtg cgtccgttat caaacagggg ggcgtttccc ctcctgccgg
     9541 ttatgattgg gttaagatta aaatgattta gtaaaatgag aaagatatgg atttaagtat
     9601 cgtagttccc atttataatg tcgaaagtta tttggaagcg tgtttaaatt ccatagaacc
     9661 catattaatc aatgaaaatg tcgaacttat tcttgtgaat gacgggtcaa aagacggaag
     9721 tgaagatata tgttataaat atatagataa aacaacaaac aacaaacaac aaacaacaaa
     9781 caacaaacaa caaacaacaa acaacaaaca acaaacaaca aacaacaaac aacaccagat
     9841 accgaacacc aaacatcaaa tatatatatc aggataacca agggacggca gaagctagga
     9901 ataccggaat aaaaaattca aacggaaaat atatagtctt tattgattca gatgatttta
     9961 ttaattgtca ggtcttgctg gattttctta gtaaagatga tattgatatg ccggatgtgg
    10021 tttttttaaa tgcggttaaa tatgataagg gaagtgtttc acattttggc gaagattatc
    10081 agcctgaaaa aatactcaag caatccaaag tcgaagtttt gaaaggatta tgccgattta
    10141 gaaaatttcc gggttcggcg tgtaataaga ttataaaaag ggaattgatt atcaaagaaa
    10201 aactgttttt tgaaaaagag gtttatgccg aagatatcga atggtcaatg agattattta
    10261 atgcggcaac aactttttct tatttggacg gttgttatta ctattatcgg cagggaagaa
    10321 aagattctaa aacaggaact gtttcggaaa aaagtataaa gtcattatta tatattttgg
    10381 agaaaaatgc ggaaatggaa tttgataggg atatatcaag ttatctttat tcttttcttt
    10441 cctacgaata tctcgttttg ctttttatca tgacgagtaa aaatatagag tgtgatgctg
    10501 atataaaaag aagggcgtat catttaaggt ttatgctgtt aaagtccaat aaattgatat
    10561 ataagctgat attcccgata atcacattat tcggggtcga tattacaggc aggattttaa
    10621 aagcaatcag ggggaatatt taataaatcc tttaacacta tacatcttac cgaaggagga
    10681 aaaatgaacg caatccgaac tttccaaaac cgcacgcccg aaatccacga aagctgtatg
    10741 atagacgaag cgtgcgtcgt catcggcgaa gtgtcgcttg ccgaagacgt ttccgtgtgg
    10801 ccgtgcgccg tgttgcgcgg cgatgtgaac agcatcaccg tcggcgcgcg cagcaatata
    10861 caggacggca gcgtcctgca cgtttcccac aaaaccgccg ccaaacccga agggtcgccg
    10921 ctggttatcg gcgaagacgt taccgtgggg cacaaagtga tgctgcacgg ctgccgtatc
    10981 ggcaaccgcg tcctggtcgg catggggacg acggttctgg acgatgccgt gattgaggac
    11041 gaagtgatga tcggcgcggg cagcctcgtg cctccgcgca aacgtttgga gggcggctat
    11101 ctttatgtcg gttcgccggt cagacaagtg cgcgtgctga ccgatgagga aaaagccttt
    11161 ttgaagtatt ccgccgcgca ttatgtgaaa ctgtcgaaac agtacgggat gtgaaatcac
    11221 atcggcgttc ctacgtcagc cctaaattca tgcggacggg atgcccgata gcggtatccg
    11281 atgcgccttg attttgaccg tctgcgtttg agttgcaggc aaaaatgccg tctgaagccc
    11341 ttttttcggg tttagacggc attttattgc cgattgcttt ttaaagtttg accgaatgct
    11401 cgcgcgtttc gtggaacacg atgtccggcc agcgttcttg cgtgagtccc agattcacgc
    11461 ggttgggggc gaggtaggcg aggttgccgc ccgcgtcgat ggcgaggttg cccgcgttgg
    11521 ctttttcaaa ttccgccagt tttttcttgt cgtcgcacga tacccagcgc gccgaccaga
    11581 tggatgcgct gtcgaacacg gcttccacgc cgtattcgtt ggcaaggcgc gaggtaacga
    11641 cttcaaactg caacacgccg accgcgccca aaatcaaatc cgcgccgctc attggtttga
    11701 acacctgtac cgcgccttct tcgccgagtt gttgcaagcc tttttgcagt tgcttgattt
    11761 tcagcgggtt tttgatgcgg acgctgcgga acagttcggg cgcgaagaat gggatgccgg
    11821 tgaacgccag ttgttcgcct tcggagaagc tgtcgccgat ttggatgttg ccgtggttcg
    11881 ggatgccgat gatgtcgccg gcgtaggctt cttcaaccag ctcgcggtcg tgggacatga
    11941 aggttaccac gctggaggcg gcgatttcgc ggttgatacg caggtgtttc attttcatgc
    12001 cgcgctcgaa tttgccggag cagacgcgca agaaggcaat acggtcgcgg tgtttcgggt
    12061 ccatattggc ttggattttg aagataaatc cggaaaactt cggctcgtcc ggctcgacca
    12121 tacgcacggt cgcatcgcgt ggtttcggcg cgggcgccca ttcaatcaat gaattgagga
    12181 tttcctgaat accgaagttg ttaatcgcag agccgaagaa tacgggcgtg agttcgccgg
    12241 cgaggaattc gtcgagattg aactcgttgg aagccgcctg caccaattcg atttcgtcgc
    12301 gcaactgttg gatttccaat ggaaagcgtt gttccaattc gggattttct atgcctttga
    12361 tgatgtcgaa ctcgtgcggc aggcgttcgc cgcccgcttc aaagagatag atttcgtcgt
    12421 tcaggatgtg gtatacgcct ttgaagtttt tgcccatacc gatcggccag gtaacgggcg
    12481 cgcagcggat ttgcaggatg ttttccactt cgtccaagag ttccagcgaa tcgcgcactt
    12541 cgcggtcgta tttgttcata aacgtaacaa tcggcgtatc gcgcaggcgg cagacgttta
    12601 agagcttgat ggtttgcgct tccacgcctt ttgccgcgtc gatgaccatt aatgcgctgt
    12661 ccacggcggt taaaacgcgg taggtgtctt cggagaagtc ttggtgtccc ggcgtgtcca
    12721 agaggttgac ggtgtggtct ttgtagtcga actgcatcac gcttgatgcc acggaaatgc
    12781 cgcgctgctt ctcgatttcc atccagtcgg aagtggcgaa tttgccggtt tttttgcctt
    12841 ttaccgtacc cgcgctttga atcgcacctg aaaacagcaa gagtttttca gtcaacgtgg
    12901 ttttacctgc gtcagggtgg gagatgatgg caaacgtgcg gcggcggcgc acttggtcga
    12961 ggatttcttg ggacatggtt ttctttgcaa aaaggttcag gccgcttttc agacggcccg
    13021 gacagtgttt gagacggcga aattgtacaa aaaaatgcct gataattcaa tgttggaggc
    13081 ggtcagtgcg tgctgccgta aatctctttt tcgtctttca ggacggcatc ggcggtttcc
    13141 cacgcgctgc cacgccagac tttgtaaaag cagctttctc gcccggtgtg gcaggcgatg
    13201 ccgccgtttt gggcgatgag catcacaatg gcgtcgccgt cgcagtcgag gcgcagttcg
    13261 cggacttttt gcgtgtgtcc cgactcttcg cccttcatcc attgtttttg gcgcgaacgg
    13321 ctgtaatagt gggcaaagcc ggtttcgacg gttttttgca gggcttcggc gttcatccac
    13381 gccaccatta aaacacgttt ggtttcggca tcttgggcga tggcgcaaac caaacctttt
    13441 tcgtcaaatt tgacggcttc aagcaggttt ttatccatat ttcctttcag acggcatggt
    13501 cggggcggtc agaggcgcac ttcgatgccg gcttcgcgca tagcgcgttt ggcttcgcgg
    13561 atggcgattt ccccgaaatg gaaaatgccg gcggcaagta cggcatcggc tttgccttcg
    13621 gttatgcctt caatcaggtg ccggacattg ccgaccccgc cggaggcgat gacgggaatg
    13681 tcgacggctt cggcaacggc gcgggtcagc ggcaggttga aaccctgttt cgtaccgtcc
    13741 ctgtccatac cggtaagcag gatttcgccc gcgccgcgtt tttgcatttc gatcgcccat
    13801 tccaccgcat ccaaaccggt cggattgcgc ccgccgtggg taaagatttc ccagcgtgtg
    13861 ttttcggggt tggcggcttt ggcatcgacg gcggcgacga tggcttgcga accgaaaaat
    13921 ccggcggctt cgtcaattaa atcgggacgg gtaacggcgg cggtgttgat gctgactttg
    13981 tccgcgccgg cattgagcag gcggcggatg tcggcaacgg tgcgtacgcc gccgccgacg
    14041 gtcaggggga tgaagacttg tccggcaacc tcttcgatga tgtgcaggat ggtgtcgcgg
    14101 ttgtcggatg aggcggtgat gtcgaggaag gtcaattcgt ccgcgccttc gccgttgtag
    14161 cgtttggcgg cttcgacggg gttgcccgcg tcgcgcaaac cgatgaagtt cacgcctttg
    14221 acgacgcgcc cgtcttttac gtcgagacag gggatgatgc gttttgccag tgccataatc
    14281 ggatgccttt agtcgaggga atctgccagt tgctgcgctt gggcaaaatc gatgctgccc
    14341 tcgtaaatcg cgcggccggt aatcgcgcct gctacgccat gtttttcggc ggcacacagg
    14401 gcgcggatgt cgtccaagcc ggtcagtccg ccggaggcga tgacgggaat gcggacggct
    14461 tgggcgagtt tgaccgtcgc gtcgatgttc acgccgctca tcataccgtc gcgcccgatg
    14521 tcggtgtaga tgatgctgtt gacaccgtcg tcttcaaagc gttttgccaa atcaattaca
    14581 tgatgcccgg ttacggttgc ccagccgtcg atggcggcca taccgtcttt ggcatccagc
    14641 ccgacgataa tcctgccagg gaaggctttg cacgcctcgc gcacgaagtc ggggtttttg
    14701 actgccgccg tgccgataat cacgtcgttc aggcccaaat ccaaatattg tccgatggtt
    14761 ttcaaatcgc gtatgccgcc gccgagctgt acggggatgt ctcgggcgac ggcggcaagg
    14821 atgtctttga tggcgggcag gttttgcgga acaccggcaa acgcgccgtt caaatctacc
    14881 agatgcaggc ggcgcgcgcc ctgtttgaac cagtgcagcg cggtttcggc gggcgaatcg
    14941 gaaaagacgg tcgcctcttc catcagccct tgtttcaggc ggacgcagcg tccttctttc
    15001 aaatcgatgg cgggtatcag cagcatagtt tttttccttg tgcggggccg tgtccggctt
    15061 accagtttaa aaagtttttc aacatcgtca gcccggcatc gtggcttttt tcggtgtgaa
    15121 attgcgtggc gaatacgttg tctttgccga cgatgcaggc aaacggggac gggtagtcgc
    15181 tttcgcccaa tatggtttcg ggattttcgg gggcgaaata gtagctgtgg acgaagtaaa
    15241 aacgcgtgtc ttgggggatg ccttgaaaaa gcgggtggtt ttgggtttgg cgcacggtgt
    15301 tccagcccat atgcgggact ttcagacggc atccctgccg gtcgcggagg tcgcgggcaa
    15361 agcgtctgac tttgccgccg aaccagccca agccgtcggt gtttccttct tcactgtggt
    15421 cgaataaaag ttgcgcgccg acgcagattc cgaaaaaggg tttgtttttt aaagcatctt
    15481 tgactgcctc gtccaagccg tcgcgcgtca gtgccgccat acagtcgggc atcgcgccct
    15541 gaccgggaaa aatgactttg tcggcgcggg acacgcggtc ggggtcgccg cttaaaaaga
    15601 tttcggtatt ttttccggca agctgccccg ccgtccggac ggatttcaat acggaatgca
    15661 ggttgcccat accgtaatcg ataatggcga tttgcatggc ttcctcctct ttttttgcaa
    15721 tatggctgcg attttaacaa acaaatgtgc cgggctgata aaaatgccgt ctgaaaacgg
    15781 gagtctgtct tcagacggca tagggtttaa acccggaaag ccgtttgtca gccttccatt
    15841 tgttttgcct gaacggcagt cagggcgatg gtgaacacga tgtcttccac cagcgcgccg
    15901 cgcgacaggt cgttgaccgg tttgcgcagg ccttgcagca gcgggccgac actcaagacg
    15961 ttggcgctgc gttggacggc tttataggtg cagttgccgg tgttcaggtc ggggaagacc
    16021 aaaacggttg cctgtcctgc gacctggctg cccggggctt tggatttgcc cacgcccggc
    16081 acggttgccg catcatattg cagcgggccg tcgatggcga ggtcggggcg tttttcccgg
    16141 gcaagtttgg ttgcttcgat gacggtatcg acatcggggc cgctgccgga gttgacggtg
    16201 gagtaggaaa tcatcgccac tttcgggtcg atgccgaagg cttttgcgga atcggcagac
    16261 tggatggcga tgtcggcaag ctgttgcgcg gtcgggttcg gattaaccgc gcagtcgccg
    16321 aagacgagga cttggttggg cagcagcata aagaatacgc tggacacgag gcttgcgccc
    16381 ggtgcggttt taatcagttg caaagcgggg cggatggtgt tggcggtggt gtgaaccgca
    16441 ccggatacca aaccgtccac atcattttgc gccatcatca tcgtaccgag taccacggtg
    16501 tcttgcagtt gcttgcgcgc gtcttcgggt gtcaggcctt tggatttgcg cagttcgcac
    16561 atcggctcga cgtattgttc gaccaatgag gcgggatcga tgatttccaa agagtcgggc
    16621 aggctgatgc cgcgttcttt ggcaacggct tcgacttctt cgcgtttggc aagcaggacg
    16681 cagcgggcaa tgcctttttc gtggcagatg gcggcggctt ggacggtgcg gggttctgcg
    16741 ccctcaggca ggacgatgcg tttgtcggct tggcgggcga agtcgatcag gttgtagcgg
    16801 aattgcgccg gcgacaggcg ttttgcttcg cggcctgcca atacggatac gtctttcagc
    16861 gcgtcgctcg aaccgaagaa ggtcaggccg gttttttcgg ctgccgcttc ggcaacggag
    16921 gctgccgcgc cgtccacgac aaaaccttcc aatacgcccg gcgcggcggc gaagaactgt
    16981 ttggcaaggt tcagccgatt tgccagttcg tcggcatcgg cgttgtcgga acggacggcg
    17041 aagacggctg ccgcgtcaag ggacaatgcc agttcgacgt ttttgcctgc gaggtagatt
    17101 ttgtcggcat cgggcgcgat gccttcgatg acgaggttgg cggcatcgag tgcggcaact
    17161 ttgccgacca gtgcgtcgaa ccagtcgtcg cttttgcctt gcgcgagcag ggtttcggcg
    17221 gttgcgtcaa cggcttggaa aatttgtgcg tccagtgctt ttgcaaaggc ttgtgcggcg
    17281 gcggaggcgt ccagtccggc agatacgggt acgatgagta cttttgccat gatatatcct
    17341 ttcgtatgct gcggtgtgcg gcatatgtgg ttggaagggg cggcatatag gcagaaacgg
    17401 ctgcctgcgt gccgtgcgtg ccgtgtttgg cttgaggcgc gcaggttgaa tatagcaaac
    17461 aaattctgtt tccaacaaga taaatatccg caggcttgtg gatgctgccg cctttcagag
    17521 ggtatttccg gggaagaaca gggcgggacc gtccaaatgg aggacggcgg aaatgccgtc
    17581 tgacagggtg ggggcggaag ggaggttgag cgtgaggacg gtttgtccga cccggagact
    17641 gatttcggta tgccgcgctt tgggcgtggt tttgagaacc acggcgtgaa tggaggcggt
    17701 gggtgcggaa tgggggtgaa ggctgaactg ttccggacgg atgagcagtg tgccgcgtgt
    17761 gcctgcgggt gcgccgcttt gaacgggcag gcggcccaat ccgcaatcgg cggtgccgtc
    17821 ggcgttgagc gcggcgggga acacgatgcc ttcgccgata aacagggcgg catcaaggtc
    17881 ggcaggttgt cggtacaatt cgtgagggct tgcagtttgg aggatgcgcc cctgtttcat
    17941 cacggcaatc cggtcggcgt attgcagggc ttcttcgcgg tcgtggctga cgaaaacggc
    18001 agatttgccg ttggcgcgca gggcggcaat catgtcttcg cgaatctggc ggcgcaactg
    18061 ttcgtccagc gcgctgaagg gttcgtccaa caaaatcagt tcgggatcgg gtgcaagggc
    18121 gcgggcgagg gcgacgcgct gttgctgtcc gcccgaaagt tcgtgcggat agcgtccggc
    18181 aagttcggaa atgccggtca attccaacat agcttcgatg cgctgccgtt cttgcgccgt
    18241 cttgcctttc ccgttgccca gcccgtaggc ggtgttgcgg taaacggtca ggtgggggaa
    18301 cagcacacct tcctgtacga cataacccaa acggcgttcg cggacgggga ggttggtatt
    18361 tttcgagaag atggttctgc cggaaagcga aatttcgcca aaatcgggtt gttcaaaacc
    18421 ggcaaggcag cgtaaaaggg tggttttgcc gcagccggac gcgccgacga taaagaggat
    18481 ttcgcccggg tcgaggctga gcgaaatgtc gtttaaaacc ggggtgtttt gaaaactttt
    18541 ggacaggtgt ccgatgtgca gggcggcggt catggcggta cttcctcaag ctgttatttg
    18601 aaggcgtatt tcttcagcag gaatacgggg atgccggaaa ataataccag catcagcgcg
    18661 taaggggtgg cggcggcgta ttgtgcgtct gatgtgtatt cccaaacggc ggtggagagt
    18721 gtgtggacat cgtcggtggt cagcagcagg gtggcggtca gctctttcat cagtttgagg
    18781 aagacgagtg cgaatgcggc ggtaatgccg ggcaggatgg acggcagtac caacgtcctg
    18841 aaaataaaga agtgtccgcg ccccaatgtt gcgccgacct gttccatccc ttttgggagt
    18901 tgttccaagg aagtcctcag ggtggtttgc gccatcggca ggtaaagcat gaaataggca
    18961 aggatgacga cgataaaggt ttggtaaacg gcaggggtgt agttgatgct gaaataaacc
    19021 aaggataggg cgataaccaa accggggacg gcgtgcagta aaaacggcag cctgtctatc
    19081 caaacggtta aaaaattgcg atagcgaacc gatgcccaaa caaggggcaa ggcacataat
    19141 atagtcaaaa tcgcacctaa agccgatacg cttaaggaac ggataaaggc atcaaatacg
    19201 gatacgagcg cgaatgtgcc ggaagtgccg accatcatcc aatgtatcaa tacgccaaag
    19261 gggataataa tgcccaaagt caacaagctg cttaaaaaaa caatcgcgcc gatctgaccg
    19321 ggcagtttga gggttttgac gggataagga cgggcaacgc ctttgccgct gtggtaaatc
    19381 ttggctttgc cgcgaaatat gctttctcca aatacgacga tgccgcacac cgccattaaa
    19441 acagcggaaa gcagggcggc ggtattgttg ttgtaggaca tttcgtattc ttggaaaatg
    19501 gcggtggtaa aagtggggta gttcaaaatg gataccgcgc caaattcgac cagcatatgc
    19561 agggcaatca gtaacacgct gctgccgatg gcgggtttga gctgggggag gatggcggaa
    19621 aaaaaggttt gcaggcggct tttgcccaag gacaggctga cttcttcgta agacaggctg
    19681 atgcgtttga gtgccgcctc gacgggcagg taggcgagcg ggaacgagga caggctcata
    19741 atcatcactg tcccccaaaa gccttcgaca cggaaggtca ggctgatcca ggtgaaacag
    19801 ctgacaaatg cggggatgca caaaggcagg gtgattgccg tctgaaaaaa ggttttgccg
    19861 aagaagcggt aacgttggaa caaaagggcg caggcaatgc ccaaaacaat ggaaatcagg
    19921 gtaacgcccg ccatcatcgt caaggtgttg gagagcaaat cccacatacg cgggcggaac
    19981 aacagttcga cggcgcggtt gatgccgacc tgccacgaac gcatagcgac atataaaaaa
    20041 ggcagggtaa gcggtagggc aatcagtagg atgaggccgg taagccaaat gggtattttt
    20101 ttaggagaca tagtgttttt tatcggcaaa acgggcggac agtataaatg tccacccgtt
    20161 tgacaatccg aaaacggctt atttcatacc ggcttgctca agcagccggg tggcgtgttc
    20221 tttttcggaa acagtggtgg cggacacttg gggtgcttcc aacttggcga tgggttccaa
    20281 attgaaggtg gataccacgt gcggattcaa aggatattcg gcacggacgg cggtcagggc
    20341 acgctgtcct tccttgctgg cgaggaaggc gacgaatttt ttcgcctcat ccttgttttg
    20401 ggaggatttt aacacggctg cgccggaata ggtaacgagt gcgccgggat ctctgtggcg
    20461 gacgaaattc aggcgggtgt ggacattttg tacgcctttt tcacgcgcga aagcgtgcca
    20521 gtagtagttg ttgatgaggg cggcatcgat ttcgccgttt tcaaccgctt gaagggcgac
    20581 ggagttttta gcgtaaggct tgccgtattc tttcagacct ttgagccatt tcaatgcggc
    20641 cgcttcgcct ttcagtttga cgatggcgac aacctgttcc aagaacgcgc cggaagtggg
    20701 ggcgtaaccg atgcggtttt tccatttcgg cgtggcgtaa ttcaggacgg atttttccaa
    20761 atctttttca gacagtttgc gggtgtcgta aacgacgacg cgcgaacgtc cgctcagtgc
    20821 cacccagtct tttttggcgg caaccggcac gcccttgccg cgtgtttcgt tgatggtgga
    20881 ggcgggcagg ggctctagga ggttggctgc ggaaagggtg gcgagtgccg ggatttgttc
    20941 ggaatagaat acgtcggcgg ggcttcggct gccttcttct ttgatttgac cggcaagctg
    21001 gtcgcctttg gcgctgttga gtttgacttt gatgccggta gcccgggtaa aggcatctgc
    21061 aacggcttgt gccgcttctt tgtgttggcc gttgtacacg gtaatgtctg ccagcgcggg
    21121 ggttgcggcg gtcagggctg cggcaagcag tgcgtatcgg atagatgttt tcatatcgat
    21181 tttctcctaa tgaatgagag tgtacacctt gttaaaacat aacggtgtgt agtgtattcc
    21241 ttcttcttta taaatgcaaa taattatttt ttaaatttgt tattatccga tccggttatg
    21301 gtttgttctg acttgtattt tttccgtgca tcgcgcccgt aaggcggaag cggcgggcaa
    21361 tgcgtggcgg aatgtgggta aaggcggcat tttgatttgt cggaatgctt gagaacccct
    21421 ctctttaaaa cagcgtctga acagggttgc cggaatagta ttgccatccc ggcggataca
    21481 gtttgtcggg gtcttgccaa tattgttcat ccagactgtt cggcagtgag gcggttttgt
    21541 tgtcgagatg ttttgttata tcccacattt ctttcaggtt tttaccttcc gattggaggt
    21601 ggcggatttc ttttctcaat gcgtctgccg cccgatggat cagcattgcg ctgtgtatcg
    21661 cgccgatttt ttcagaacgg aacaagtctt ttcggcggcg ggaaaacccc aattgcagac
    21721 aaattgcagt atgccgccgt tgccgatgtc ggcttccgcc cgccaaatca aaaccagctc
    21781 ctgttcacgt ccgtccatgc tttcgagttt gccgtcatgc tgttcaaaaa gtttgtctac
    21841 cgcctgccac atcatctgtt cgaagcggtc ggcttgggta tccgtatcgg tcatggtgtt
    21901 cttgtctttt aaaaatgccg tctgaacggc ttcagacggc atcgggggtt aagccaacac
    21961 ttcacgccag cgtttgactt ggaaacggac ttgttcaggc gcggtgccgc ccaagtggtt
    22021 gcgcgcgttt aagctgcctt cgggtgtcag cacgccgtaa acgtcgtcgg caatcaaatc
    22081 gctgaaacct tgtaagactt cgagcggcag ttcgctcaaa tcgacgcccg cttggtcggc
    22141 gtggcgcacg gcttgggcga cgacttcgtg ggcatcgcgg aaaggcatgc cttttttgac
    22201 cagataatcc gccaagtcgg tggcggtagc gaagccctgc atcacggcgg cgcgcatatt
    22261 gtcgggtttg acggttacgc cgcgcatcat atcggcgtaa atccgcaacg tgtcgataag
    22321 cgtgtcggcg gtgtcgaaca agggttcttt gtcttcctga ttgtctttgt tgtacgccaa
    22381 gggttgggat ttcatcaggg taatcagacc gataaggtgt ccgatgacgc ggccggattt
    22441 gccgcgcacg agttcgggca cgtcgggatt tttcttctgc ggcatgatgg acgaacctgt
    22501 gcagaaacgg tcggcgatgt cgataaagcc gaaacgcgga ctcatccaca aaatcaattc
    22561 ttcagacagg cggctcaggt gaaccataac cagcgaggcg gcagccgtga actcgacggc
    22621 aaaatcgcgg tcggatacgg catcgagcga gttttggcag atttgctcga aacccagtaa
    22681 ttcggcggta atttcgcgcc gaatcgggta ggtcgtaccg gcaagggcgg ctgcgccgag
    22741 cggcatacgg ttgacgcggc ggcggcagtc cgccatccgt tcgttatcgc ggccgagcat
    22801 ttcgacgtag gcgagcatat ggtgtccgaa gctgacgggc tgggcgactt gcaggtgggt
    22861 aaagccaggc ataacggttt cggcgttttg ttccgccaaa tccagcaatg ccgtctgaag
    22921 gttttgaatc aggttttgaa taacggtaat ctgatcgcgc agccacaggc ggatgtcggt
    22981 ggcgacttgg tcgttgcggc tgcggccggt gtgcaggcgt ttgcccgcgt cgccgatttt
    23041 gtcggtcagg cggcgttcga tgttcatgtg gacatcttcc aaatcggacg accattcgat
    23101 tttgccgctg cggatttctt cgaggatttc cgccatgccc cgacggatgt ccgccaaatc
    23161 gccgtcatcc aacacgccgg tttctttcag catttgcgcg tgggcaagcg agccttggat
    23221 gtcccattcg gcaaggcgtc ggtcgaaacc gatggaggcg gtgtattgtt tgacgagttc
    23281 ggaaacgggt tcgttgaaac gtccggacca ggttttgtcg tgcataagga ttccttgatg
    23341 tggttattcg gtacggtatt tttccaaaag gcggcggaag ggttcgccgg tttcggggtg
    23401 tttcaggccg taggcgacgg tggcttccag atagcccagt ttgctgccgc agtcgtagcg
    23461 cgtgccttca aaggggtgcg ccaggacaaa ttcgtgatcg agcagcttgg cgatgccgtc
    23521 tgtaagctgg atttcgttgc ccgcgccgcg cggcagtccg gtcagtaagt cgaaaatgcg
    23581 cggggtaagg atgtagcgtc cgacgacggc aaggttggag ggcgcgtctt cgggcttggg
    23641 tttttcgaca atgccgataa tgcgttggaa ctgtttgagc tgttcggttt cgacgatgcc
    23701 gtatgagccg gtttgcgatg cttcaacggt ttctacgccc aaaatgctgt tgccgctgcg
    23761 cccgtacact tcgaccattt gtttgagcgc gcctttgggg gcatcaatca ggtcgtcggc
    23821 aaggataacg gcaaagggtt cgtctccgat ggcggcgcgg gcgcacaaga cggcgtgtcc
    23881 caagcccagt gcttccgcct gacggatgta gaggcaggta atgttcggcg gcaggatgtt
    23941 gcggacgtgt tccaacaatt tgtctttatg gcgcatttcc aactcggttt cgagttcgta
    24001 tgccttgtcg aaatggtctt cgatgctgcg tttgttgcgt ccggtaacaa acaccatttc
    24061 cgtgcagccg gcttccacgg cttcttctac ggcgtattgg atcagcggct tgtcgacgat
    24121 gggcagcatt tctttcgggc ttgccttggt ggcgggcagg aagcgggttc ccatccctgc
    24181 gacggggaag acggctttcc gtatgggttt cattcttttt cctttgtatt gttttgatgt
    24241 ttaaagggcg agtttgcgta agagttcggc aagtgcctgc gcgcggtggc tttcgcggtt
    24301 tttgacctcc gaatccaatt cggcggcggt tttgccgtgt ccgggcagat aaaaatacgg
    24361 gtcgtaaccg aaaccgtttt caccggcggg aacgccgttc cattgtccgt gccatacgcc
    24421 ttcggcaata atcggacgcg ggtcgtcttt atggcggaca aaaaccaata cgcagacgta
    24481 gcaacagctt ttgtcggctt tgccggcaag ttcggcggca agtttcaggt tgttggcggt
    24541 atcggatttg ggattgctgc cggcgtaacg tgcggaatgt atgcccggag caccgtttaa
    24601 ggcggcggca cagatgccgc tgtcgtcggc gagtgcgggc agcccgctgt gtccggcggc
    24661 gtgccgggct tttgccagcg cattttcaac aaaggtggaa tagggttcgg ggcattcggg
    24721 tatgctgaac tcggattgcg gcaatacggt aatgccgtat ggttcgaata ggttgccgaa
    24781 ttctttgagt ttgcctgcat tgccgcttgc caaaacgatt ttttccggtt tttcagacat
    24841 agcggttttc ctttgcggcg gattgggcgg cgcgtaggga tttgtgccgc aggtagaggg
    24901 cgaggctgcc gatttgtccg aacagtgcgc cgaatgcgaa aagaaaggag gcgacggcga
    24961 aggctttgct gccgacggtc agcagataag cacccaagag gacgagcagc ataaatgcca
    25021 gtgtgaagag ggcaacagac aacagataga tttttcggcg gttcatggcg ttcggtcgga
    25081 aacggtatgt tcggattata gccgattggg acggtattcc ctagagcttg gaaaaatgcc
    25141 gtctgaaacg gcgttcagac ggcatggcgg gcgttatgcc tgtttgttcc aacgttcgat
    25201 ggattctttg atgacttttt tcgcttcttc cgcatcgccc cacgattcga ctttggtcgt
    25261 acctgctttt ttcaggtctt tgtaatggtt gaagtggaac tcgatttgtt tgatgagctg
    25321 ttgcggcaaa tcggacaaag ttttgtaggc gttgccgttg ttgcggtcgt cggcaggaac
    25381 gcagacgatt ttgtcgtcca cttcgccgtc gtcaacgaat ttcatcacgc cgataacgcg
    25441 cgcttccaag aatacgccgg ttgccaaagg ttgttcggta acgagcagca cgtccaattc
    25501 gtcgccgtct tcgtccaaag tttggggaat gaagccgtag ttggtcggtt tggcgaagat
    25561 ggcgggttcg acgcggtcga gttggaatgc ggccagtttg cggttccatt cgattttgtg
    25621 gttgctgccg gcggggattt cgttgacaac gttgatgatg ccgccgtcca cgtcgccggg
    25681 ggtcaggatt tggttgaagt ctgccatttg gtttcctttg tttgggaaag tgtgaagttt
    25741 gaaagtatag cacaaacgtc cggctgaaaa tgcgcccgat gcctctgaaa gggtgtacgg
    25801 gcgcgtgtta ccgtttgccc aaaaacctgc ccagttccaa aatcgcgcgc ctgttggacg
    25861 gggaaaatac tttttccgct gcttcttcca tatcgaacca accgtaggag acgtgttctt
    25921 cgggttgcag ggcgatgggc gtatcacgcg ggatttcggc agagaagagg tgttcgcggt
    25981 tttcaaacac gccttttgga tagcggtgcc gccagtggtg gtagatttcg taaaccgtgc
    26041 tgtcgtgcca gtcttgaagc tgcccgtccg ccagcaggat gccggtttct tcccaaactt
    26101 cgcgccttgc cgtttgggcg acggtttcgc ccggttcgag gctgccggtt accgactgcc
    26161 aaaatccttc cggatgcgtg cgttcgatga gcaggatgcc gccgtccccg ctataaagga
    26221 cgaccagtgc ggaaacgggg tatttgagcg gttttgccat cggcatcttt cggcgggctg
    26281 cggtaatgaa ggggcggatt atagcaaacg ccgcacgtta tggcgtttat ccttttccgt
    26341 atcctttttt ctgcacggat gggacgcgcc ggtgtttgcc ggtaaatttt ccgttgtgtc
    26401 aaaaagataa gggcggttgt gattttaatg cttgccaaag cgtcgggcgg aaactataat
    26461 ccgaaactta tcgagtcgga gtgtggcgca gtctggtagc gcacttgcat ggggtgcaag
    26521 gggtcgaagg ttcgaatcct ttcactccga ccaaaaattc cgaaagccgc tttcaaaagc
    26581 ggcttttttg ccgtccgtat gattatgatg tagagtacgc ggcgacagac attcaaatgc
    26641 cgtctgaaaa ccgttcagat ggcatctctt tatcttagtt tcattccgta ccatcttaag
    26701 gaacatcaaa ttgggcattt cccgcaaaat atcccttatt ctgtccatac tggcagtgtg
    26761 cctgccgatg catgcacacg cctcagattt ggcaaacgat tcttttatcc ggcaggttct
    26821 cgaccgtcag catttcgaac ccgacgggaa ataccaccta ttcggcagca ggggggaact
    26881 tgccgagcgc agcggccata tcggactggg aaaaatacaa agccatcagt tgggcaacct
    26941 gatgattcaa caggcggcca ttaaaggaaa tatcggctac attgtccgct tttccgatca
    27001 cgggcacgaa gtccattccc ccttcgacaa ccatgcctca cattccgatt ctgatgaagc
    27061 cggtagtccc gttgacggat ttagccttta ccgcatccat tgggacggat acgaacacca
    27121 tcccgccgac ggctatgacg ggccacaggg cggcggctat cccgctccca aaggcgcgag
    27181 ggatatatac agctacgaca taaaaggcgt tgcccaaaat atccgcctca acctgaccga
    27241 caaccgcagc accggacaac ggcttgccga ccgtttccac aatgccggta gtatgctgac
    27301 gcaaggagta ggcgacggat tcaaacgcgc cacccgatac agccccgagc tggacagatc
    27361 gggcaatgcc gccgaagcct tcaacggcac tgcagatatc gttaaaaaca tcatcggcgc
    27421 ggcaggagaa attgtcggcg caggcgatgc cgtgcagggc ataagcgaag gctcaaacat
    27481 tgctgtcatg cacggcttgg gtctgctttc caccgaaaac aagatggcgc gcatcaacga
    27541 tttggcagat atggcgcaac tcaaagacta tgccgcagca gccatccgcg attgggcagt
    27601 ccaaaacccc aatgccgcac aaggcataga agccgtcagc aatatcttta cggcagtcat
    27661 ccccgtcaaa gggattggag ctgttcgggg aaaatacggc ttgggcggca tcacggcaca
    27721 tcctgtcaag cggtcgcaga tgggcgcgat cgcattgccg aaagggaaat ccgccgtcag
    27781 cgacaatttt gccgatgcgg catacgccaa atacccgtcc ccttaccatt cccgaaatat
    27841 ccgttcaaac ttggagcagc gttacggcaa agaaaacatc acctcctcaa ccgtgccgcc
    27901 gtcaaacggc aaaaatgtca aactggcaga ccaacgccac ccgaagacag gcgtaccgtt
    27961 tgacggtaaa gggtttccga attttgagaa gcacgtgaaa tatgatacga agctcgatat
    28021 tcaagaatta tcggggggcg gtatacctaa ggctaagcct gtgtttgatg cgaaaccgag
    28081 atgggaggtt gataggaagc ttaataaatt gacaactcgt gagcaggtgg agaaaaatgt
    28141 tcaggaaata aggaacggta ataaaaacag taactttagc caacatgctc aactagagag
    28201 ggaaattaat aaactaaaat ctgccgatga aattaatttt gcagatggaa tgggaaaatt
    28261 taccgatagc atgaatgaca aggcttttag taggcttgtg aaatcagtta aagagaatgg
    28321 cttcacaaat ccagttgtgg agtacgttga aataaatgga aaagcatata tcgtaagagg
    28381 aaataatagg gtttttgctg cagaatacct tggcaggata catgaattaa aatttaaaaa
    28441 agttgacttt cctgttccta atactagttg gaaaaatcct actgatgtct tgaatgaatc
    28501 aggtaatgtt aagagacctc gttataggag taaataaaaa tggcaatttg gaattatcgg
    28561 tactacctta tcccatcagc tgctatcaga aataaattta atgcaaataa agatatcata
    28621 cttgatgagt atcgatctaa tggttttcag aattttaatg agaataaaag ttttgaaaat
    28681 tactttatcg ataatgatgt tatattatta tcaataataa atgaagcaaa aaaacagctt
    28741 aaattgaaag aatcttggga taaagacgca atcatgtttt gtgataattt tggtaatagt
    28801 cttaccgttt ggccagatga tatagagtgc gaacttgatt taagatttga ttatactaaa
    28861 tttattcaga aaaccattga ttgggcaata aaatataatt gtctacttgt aatagaaaaa
    28921 acaggaaatg tagtttcccc taatataaat aatctgatgt atgaaataaa agcatatttg
    28981 gaaagcaagc cgtggcccat atgaaaccta aactcaacaa gtaggatgtg tgcggaacgc
    29041 acgtatgcgg ttctcaaggt ttgagctaag aggccgtctg aaaacagaaa aactgtttca
    29101 gacgaccttt cttttaacca gttgccacag caaccggaca aaagcagcct acctccacat
    29161 ccatataggc aatacagggg agatattttg taaattctac gaatatttta tctgctaaac
    29221 agggtaggat atggtatgaa gcgaacattg gcttaataaa cactatgtca agatcgaatc
    29281 aggctggtac tagattgttg tattccaatt atggattgct atatataaca actgatcatt
    29341 atatctctgc aacacggttt gtagcttgga aataggagta taacttatgc aattagagat
    29401 tatcggtagt aaaatttata cggaacaaga ttttcataat caaatttcaa aaatattttc
    29461 tatacaagat tattatggga acaatcttga tgctttatgg gatttattaa gcacaaatgt
    29521 agaacgaccg attactttgg tatggaaaga tgctatgttc tcaaaaaatc aattagaaaa
    29581 tatatttatt gaaatcgtaa atgttctaga aagagttaag aaacaagatg aggattatgg
    29641 attcgaagaa aaatttaatt atattttaga gtaagtaaac cctaattaca ttacgtacac
    29701 aggcttaaaa ctccccagag ccaattaagc aagccgtaac ccatataaaa cttaaactca
    29761 acaagtagca tgtgtgcgga acgtacgcat gcggttctta aagtttgagc taagaggccg
    29821 tctaaaaaca gaaaaaccgt ttcagacggt ctttgtttaa cgccaccgat ccagcgggtt
    29881 acaaagcgca gtcaatgccg ctgcgcctta tgcctccgaa gcaataggca gaacatttgg
    29941 acacggtgaa aacaaaaacg aaaccgccca agccgtcgga catttccttt taggagcagc
    30001 tattgcccgc gtcaacggtg gtaattttgc tgccggcggc tcggcagcag ttgcagctga
    30061 aaaggcggcg gaacatcttg cccaacagta taacgacggt aaaaccgcaa tcgatccgca
    30121 aacaggcgag ttcaatgcca acctgctgcc ggaacatatc aaagaggaaa tcaaatcaaa
    30181 gagcggggtg attgcatcgc tgacgggcgc ggccgtgggc ggcacgccgg tagatgcgca
    30241 aaccggaggt gcggtcggac agaatgcggt ggaaaacaac ctctatctga catcggaagc
    30301 cttaaagaag gacaagcaga cagctcgtaa aatttattcc gtcataaaag agcaagtcaa
    30361 gcatgaatgc agttccacag gaagaattac cgaatgtcgt caaaatatag gacgcattat
    30421 cgaatttacc caagacaaac gctttgacag taggtttaag gacttaaaaa aagaatcctt
    30481 atattaccta aataaacatc ctgatttagt agcctcttat ttgaaggctg aatacgaaaa
    30541 gctggatagg gaagacaaaa gtatcctgca ccgctacatc tcacccgggg ctgaaatcgt
    30601 ttcgggcagt ttgggggttg ttctttcagg agtagccgga ggcggatctt gtgccgagac
    30661 tttcggctta ggctgtgccg ccgctttggt tggtgtaacg tcttcctacg atcatgtcat
    30721 tactgggacg aaaaacttcg gaaaaaaagc cagcgagcaa cgaccgacga ttgcggttca
    30781 ggccttgaag cagttggggc tgtcggagca ggctgcggaa tatgttcagt tctctataga
    30841 tttgttcagt gtgggtaaat cggggggcgg tatacctaag gctaagcctg tgtttgatgc
    30901 gaaaccgaga tgggaggttg ataggaagct taataaattg acaactcgtg agcaggtgga
    30961 gaaaaatgtt caggaaacga gaagaaggag tcagagtagt cagtttaaag cccatgcgca
    31021 acgagaatgg gaaaataaaa cagggttaga ttttaatcat tttataggtg gtgatatcaa
    31081 taagaaaggc acagtaacag gagggcatag tctaacccgt ggtgatgtac gggtgataca
    31141 acaaacctcg gcacctgata aacatggggt ttatcaagcg acagtggaaa ttaaaaagcc
    31201 tgatggaagt tgggaaatga aaaaaggaag gggcggaaaa cctatgacaa atcattctat
    31261 gttcccaaaa gattgggatg aggctagaat tagggctgaa gttacttcgg cttgggagaa
    31321 aagaaaggaa ttagaaggtc aaaaatggag aggtacaagt aaatctggtg ttgaaataga
    31381 aggatttacg gaacctaatc gaacagctta tccactttat aatagtaaat gaagtattga
    31441 ttaaaaaggt gtttttaaat gtataaaata aaatgttttt atgatcagtt tagttctggt
    31501 gaattattta actactgcca gtttttagat aattcttcta atcaaaagat aaatacacga
    31561 ggaacggtgt atcaatatat tatttatgtt ttaacaggag acctttatct acaaaaagac
    31621 atagatgaaa atttagaatt tatccatcaa gcagaaaata atcccaatga agtttattca
    31681 ggaggtggac aaggtttttg ctgggatatt tctgctgaaa aagtagtttt ctatcacaac
    31741 gaatttgatg aagaagacgg ctggcctgat ttaagctgtt cgctgcatac ttttaaaact
    31801 gctttaattg cttggaatgc ttttttgcaa ttgcctaaaa gtattcattc ggtggtggag
    31861 actgtgattg aggaataagc ataattagct taatgaatag aatcagcgat atagattgga
    31921 ctgcaaatcc acgcttatac gctgtgccat gattaacagg aagagagcaa cttggatggg
    31981 ctagtaggga tggtaagcat ttaaatatat caattgatgg aaagattaca cacaaatgac
    32041 taatttaaaa ttagatttct actctgaagt tattataaaa gattcttgcc ctaatgattt
    32101 gttagaaaat ggtgagacta ttaaaggaaa aaaaggagtg gtattaggta taagcgaaga
    32161 agatggtata atctatggct atactatttt actttttgat ataaaatatt gtatatatat
    32221 agataagaaa tatattatac cgactggaaa aaaattctct cgcgatgatt tttattagct
    32281 aatttaagta atttaagcaa gttttttaga tactgaaact ggtaattatg tttatgtaag
    32341 cataaggagt tcagattgaa ggattctaca gggcaaatga ggctggcaac caaggatttg
    32401 gcggaagcca ttaaacgagg agaagtacgt agttctgctt ttacaacaaa gcaactaaag
    32461 gcaatcgaaa aaggagaatc taaaataccg gattacactt ggcatcatca tcaagacact
    32521 ggaaggatgc aattgattaa tgaagacttg catcatgata ccggccatat cggttggaga
    32581 gcgatgagta aaggaaagta actatgtgga aaatcataaa agaggatagt gatgatttag
    32641 gatttgcaat taaatgctta ttctctcaat ctattgattt aaatgaattc aaattatgga
    32701 ttgaacaagt aatacgagat atgcccatcg aggacatccc tttttatatt tttgacttgg
    32761 cggatttcga tggggtaatt gccgatattg acaatattgt aggttttgtt tcgagttaca
    32821 gcctatcaaa atcgaaaaaa aaatgccttg accggcattg ccttcttaag ggggatagat
    32881 gtctatgacc ctcccatttc aaaagaaaaa gcattaaaag ccttagagaa acatcctgaa
    32941 atttatcaga agtttcggca tttctttccg tttgtagaac tgcccccgct ttaaacagtc
    33001 aaaataccgt ctgaaagcca
//
LOCUS       DAWFHY010000015        32660 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.015, whole genome shotgun sequence.
ACCESSION   DAWFHY010000015 DAWFHY010000000
VERSION     DAWFHY010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 32660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32660
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.015"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(201..947)
                     /gene="fabG"
                     /locus_tag="WGQ68_000592"
     CDS             complement(201..947)
                     /gene="fabG"
                     /locus_tag="WGQ68_000592"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEZ0714445.1"
                     /translation="MSTQDLSGKIALVTGASRGIGAAIADTLAAAGAKVIGTATSESG
                     AAAISERLAQWGGEGRVLNSAEPETVENLIADIEKTFGKLDILVNNAGITRDNLLMRM
                     KEEEWDDIMQVNLKSVFRASKAVLRGMMKQRAGRIINITSVVGVMGNAGQTNYAAAKA
                     GLIGFSKSMAREVGSRGITVNCVAPGFIDTDMTRALPEETRQTFTAQTALGRFGDAQD
                     IADAVLFLASDQAKYITGQTLHVNGGMLMP"
     gene            complement(1009..2574)
                     /gene="guaA"
                     /locus_tag="WGQ68_000593"
     CDS             complement(1009..2574)
                     /gene="guaA"
                     /locus_tag="WGQ68_000593"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEZ0714446.1"
                     /translation="MTQDKILILDFGSQVTQLIARRVREAHVYCELHSFDMPLDEIKA
                     FNPKGIILSGGPNSVYESDYQADTGIFDLGIPILGICYGMQFMAHHLDGEVQPGNQRE
                     FGYAQVKTIDSELTRGIQDDTPNTLDVWMSHGDKVSKLPTGFTVIGDTPSCPIAMMEN
                     AEKQFYGIQFHPEVTHTKQGRALLNRFVLDICGAQPGWTMPNYIEEAVAKIREQVGSD
                     EVILGLSGGVDSSVAAALIHRAIGDQLTCVFVDHGLLRLNEGKMVMDMFARNLGVKVI
                     HVDAEGQFMAKLAGVTDPEKKRKIIGAEFIEVFDAEEKKLTNAKWLAQGTIYPDVIES
                     AGAKTKKAHAIKSHHNVGGLPENMKLKLLEPLRDLFKDEVRELGVALGLPREMVYRHP
                     FPGPGLGVRILGEVKKEYADLLRQADDIFIQELRNTTDENGTSWYDLTSQAFAVFLPV
                     KSVGVMGDGRTYDYVVALRAVITSDFMTAHWAELPYSLLGRVSNRIINEVKGINRVVY
                     DVSGKPPATIEWE"
     gene            complement(2674..4539)
                     /gene="msbA"
                     /locus_tag="WGQ68_000594"
     CDS             complement(2674..4539)
                     /gene="msbA"
                     /locus_tag="WGQ68_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HEZ0714447.1"
                     /translation="MIEKLTFGLFKKEDARSFMRLMAYVRPYKIRIVAALIAIFGVAA
                     TESYLAAFIAPLINHGFSAPAAPPELSAAAGIISTLQNWREQFTYMVWGTENKIWTVP
                     LFLIILVVIRGICRFTSTYLMTWVSVMTISKIRKDMFAKMLTLSSRYHQETPSGTVLM
                     NMLNLTEQSVSNASDIFTVLTRDTMIVTGLTIVLLYLNWQLSIIVVLMFPLLSLLSRY
                     YRDRLKHVISDSQKSIGTMNNVIAETHQGHRVVKLFNGQAQAANRFDAINRTIVRLSK
                     KITQATAAHSPFSELIASIALAVVIFIALWQSQNGYTTIGEFMAFIVAMLQMYAPIKS
                     LANISIPMQTMFLAADGVCAFLDTPPEQDKGTLTPQRVEGRISFRNVDVEYRSDGIKA
                     LDNFNLDIRQGERVALVGRSGSGKSTVVNLLPRFVEPSAGNICIDGIDIADIKLDCLR
                     AQFALVSQDVFLFDDTLFENVRYSRPDAGEAEVLSALQAANLQSLIDASPLGLHQPIG
                     SNGSNLSGGQRQRVAIARAILKDAPILLLDEATSALDNESERLVQQALERLMENRTGI
                     IVAHRLTTIEGADRIIVMDDGKIIEQGTHEQLMSQNGYYTMLRNISNKDAAVRTA"
     gene            complement(4677..5603)
                     /gene="fabD"
                     /locus_tag="WGQ68_000595"
     CDS             complement(4677..5603)
                     /gene="fabD"
                     /locus_tag="WGQ68_000595"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEZ0714448.1"
                     /translation="MSFAFFFPGQGSQSLGMMNGFAEQAIVKATFDEASAILGQDLWA
                     MINGSDAEIIGQTVNTQPIMLAAGVAVYRAYLEAGGKTPAAVAGHSLGEYTALVAAGA
                     LNFADAVKLVRLRAELMQSAVPQGVGAMAAILGLEDEQVRQICTESAQDEVVEAVNFN
                     SPGQVVIAGNAAAVGRAMTAAKEAGAKRALPLPVSVPSHCSLMKPAADKLAEALKTVE
                     IKQPQIRVIHNADVAAYDDADKIKDALVRQLYSPVRWTETVNALVSDGIAESAECGPG
                     KVLAGLAKRINKTAACSALTDAGQIAAFIEAH"
     gene            complement(5784..6104)
                     /locus_tag="WGQ68_000596"
                     /pseudo
     CDS             complement(5784..6104)
                     /locus_tag="WGQ68_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223069.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
     gene            complement(6151..7113)
                     /locus_tag="WGQ68_000597"
     CDS             complement(6151..7113)
                     /locus_tag="WGQ68_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEZ0714449.1"
                     /translation="MQYAKISGTGSYLPANRVSNDDLAQKVDTSDEWITARTGIKFRH
                     IAAENEKTSDLAAEAARRALDAAGLDSGEIDLIIVATATPDMQFPSTATIVQQKLGIT
                     NGCPAFDVQAVCAGFMYALTTANAYIKSGMAKNALVIGAETFSRIVDWNDRTTCVLFG
                     DGAGAVVLSASDTPGIIHSKLKADGNYLKLLNVPGQIACGKVSGSPYISMDGPGVFKF
                     AVKMLSKIADDVIEEAGYTAAQIDWIVPHQANRRIIESTAKHLGLSMDKVVLTVQDHG
                     NTSAASIPLALDTGIRSGQIKRGQNLLLEGIGGGFAWGAVLLQY"
     gene            complement(7140..7343)
                     /locus_tag="WGQ68_000598"
     CDS             complement(7140..7343)
                     /locus_tag="WGQ68_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714450.1"
                     /translation="MPSEACLVFRRHFLRLKQRIGNPALPIHGNLHLKTICIPNHTPI
                     PVSVICTLSVLGNHRFFAYNSRI"
     gene            complement(7363..7779)
                     /locus_tag="WGQ68_000599"
     CDS             complement(7363..7779)
                     /locus_tag="WGQ68_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714451.1"
                     /translation="MKTSTVVFGGFFMADNRERIQIPVLENPGIREINHFFSVSNFEK
                     KAGVLVFRIIPEPKFGNTELTIYFEEGCYLPMIQTILEDGDIEVKNLKTENYSGNTME
                     IWGDFYPIEHISKNISTIQNIISEFMIQKQTPAPII"
     gene            complement(7806..8057)
                     /locus_tag="WGQ68_000600"
     CDS             complement(7806..8057)
                     /locus_tag="WGQ68_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0714452.1"
                     /translation="MKTLEKRMKALDKQMMKFGKSLEGRLDARLIESALDYIHYSERF
                     LAFEILCTYIEDFDVRLTEQESREISFINKEFEIESTSD"
     gene            complement(8193..9248)
                     /gene="plsX"
                     /locus_tag="WGQ68_000601"
     CDS             complement(8193..9248)
                     /gene="plsX"
                     /locus_tag="WGQ68_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEZ0714453.1"
                     /translation="MITLAVDAMGGDQGLAVTVPGATAFLQAHPDVRLIMTGDETQLR
                     QALTAAGAPMERIDICHTAQIVGMDEAPQSALKNKKDSSMRVAINQVKEGKAQAAVSA
                     GNTGALMATARFVLKTIPGIERPAIAKFLPSDTDHVTLALDLGANVDCTPEQLAQFAV
                     IGSELVHALHPQKGQPRVGLVNVGTEDIKGTDTVKQTYKLLQNSKLNFIGNIESNGIL
                     YGEADVVVADGFVGNVMLKTIEGAVKFMSGAIRREFQSNLFNKLAAVAALPALKGLKN
                     KLDPRKFNGAILLGLRGIVIKSHGGTDETGFRYALEEAYHEAKSAGLSKIEQGVAEQL
                     AALETAKAVQNENADGL"
     gene            complement(9336..9863)
                     /locus_tag="WGQ68_000602"
     CDS             complement(9336..9863)
                     /locus_tag="WGQ68_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HEZ0714454.1"
                     /translation="MKQKIWYTYDDIHRVIKALAEKIRNADIKYDAMIAIGGGGFIPA
                     RMLRCFLEIPIYAVTTAYYDSDNEGQVTEEVKKVQWLDPVPEALRGKNVLVVDEVDDS
                     RVTMEFCLTELLKEDFGTIGVAVLHEKIKAKAGKIPEGIPYFSGITVEDWWINYPWDA
                     LDIDEHNRLAEAGRG"
     gene            complement(10038..10217)
                     /gene="rpmF"
                     /locus_tag="WGQ68_000603"
     CDS             complement(10038..10217)
                     /gene="rpmF"
                     /locus_tag="WGQ68_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009889986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEZ0714455.1"
                     /translation="MAVQQNKKSPSKRGMHRSHDALTAPALSVDSTTGEVHRPHHISP
                     NGMYRGRKVVKAKGE"
     gene            complement(10251..10754)
                     /locus_tag="WGQ68_000604"
     CDS             complement(10251..10754)
                     /locus_tag="WGQ68_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003776445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEZ0714456.1"
                     /translation="MSDPNLIDPEIFAAEGQNLQGSFLLEELDERVSSHDYPADRQTK
                     ISFTLTGGRDRLQRLFLDLNVKADMPLICQRCIKPMPFMLDESSRIVLFSNEESLDES
                     MLADEELEGILIEKELDVRTLVEDQILMSLPFSPRHEDCGDNGTLEEVNRDKPNPFAV
                     LAGLKSS"
     gene            10902..11492
                     /gene="maf"
                     /locus_tag="WGQ68_000605"
     CDS             10902..11492
                     /gene="maf"
                     /locus_tag="WGQ68_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation protein Maf"
                     /protein_id="HEZ0714457.1"
                     /translation="MGLELPLILGTSSVFRREQMERLGIAFQAASPDFDETPMLGESA
                     PQTALRLAEGKARSLAGRFPEALIVGADQVAWCDGRQWGKPMNLANAQKMLMHLSGRE
                     IEFYSAVVLLNTVTGRMQRHIDKTVVVMRQLDELHILRYLEREPDAVYCSCALKSEDL
                     GALLIERIESTDPNALIGLPVFRLVDFLKNEGVEVL"
     gene            11551..12264
                     /locus_tag="WGQ68_000606"
     CDS             11551..12264
                     /locus_tag="WGQ68_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEZ0714458.1"
                     /translation="MMSPVLYLIPTPLGTPDTPCLLPHEQQAIVGLTDFVVEAEKTAR
                     AHLKHLGVTMPIRELNLQTLNEHTDLKTLLELLKPLQEGRSMGIVSEAGCPAVADPGA
                     NLVALAHKHGFEVRPLVGPSSLLLALMASGANGQNFAFKGYLPSEKNERIQSLKALEQ
                     RSRQQNETQLFIETPYRNDVLLADAVENLHSETRLCTATDLTLPTQEIISQTVVQWRK
                     RKEMPNLKKRPTIFVMYAG"
     gene            complement(12345..13982)
                     /gene="yidC"
                     /locus_tag="WGQ68_000607"
     CDS             complement(12345..13982)
                     /gene="yidC"
                     /locus_tag="WGQ68_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEZ0714459.1"
                     /translation="MDFKRLTAFFAIALVIMIGWEKMFPTPKPVPAPQQAAQQQAVTA
                     SAEAALAPATPITVTTDTVQAVIDEKSGDLRRLTLLKYKATGDEHKPFVLFGDGKEYT
                     YVAQSELLDAQGNNVLKGIGFTAPQKQYSLEGDKVEVRLSAPETNGLKIDKVYTFTKG
                     SYLVNVRFDIANGSGQTANLSADYRIVRDHSEPEGQGYFTHSYVGPVVYTPEGNFQKV
                     SFSDLDDDAKSGKSEAEYIRKTPTGWLGMIEHHFMSAWILQPKGGQSVCVAGDCSIDI
                     KRRNDKLYSASVSVPLAAIPAGAKAETAVNLYAGPQTTSVIANIADNLQLAKDYGKVH
                     WFASPLFWLLNQLHNIIGNWGWAIIVLTIIVKAVLYPLTNASYRSMAKMRAAAPKLQA
                     IKEKYGDDRMAQQQAMMQLYTDEKINPLGGCLPMLLQIPVFIGLYWALFASVELRQAP
                     WLGWITDLSRADPYYILPIIMAATMFAQTYLNPPPTDPMQAKMMKIMPLVFSVMFFFF
                     PAGLVLYWVVNNLLTIAQQWHINRSIEKQRAQGEVVS"
     gene            complement(14154..14375)
                     /gene="yidD"
                     /locus_tag="WGQ68_000608"
     CDS             complement(14154..14375)
                     /gene="yidD"
                     /locus_tag="WGQ68_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEZ0714460.1"
                     /translation="MNFLLSKLLLGLIRFYQYCISPLIPPRCRYTPTCSQYAVEAVKK
                     YGAFKGGRLAIKRIARCHPFGGHGHDPVP"
     gene            complement(14440..14805)
                     /locus_tag="WGQ68_000609"
     CDS             complement(14440..14805)
                     /locus_tag="WGQ68_000609"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEZ0714461.1"
                     /translation="MDYRFGRQYRLLKTDDFSSVFAFRNRRSRDLLQVSRSNGNGLDH
                     PRIGLVVGKKTAKRANERNYMKRVIRDWFRLNKNRLPPQDFVVRVRRKFDRATAKQAR
                     AELAQLMFGNPATGCGKQV"
     gene            complement(14808..14942)
                     /gene="rpmH"
                     /locus_tag="WGQ68_000610"
     CDS             complement(14808..14942)
                     /gene="rpmH"
                     /locus_tag="WGQ68_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEZ0714462.1"
                     /translation="MKRTYQPSVTKRKRTHGFLVRSKTRGGRAVLAARRAKGRKRLAV
                     "
     gene            15234..16790
                     /gene="dnaA"
                     /locus_tag="WGQ68_000611"
     CDS             15234..16790
                     /gene="dnaA"
                     /locus_tag="WGQ68_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEZ0714463.1"
                     /translation="MTLAEFWPLCLRRLHDMLPQGQFAQWIAPLTVGEEGGVWVVYGK
                     NQFACNMLKSQFAGKIEAVREELAAGRSAFVFKPGEGVRYEMAAVEGAVEPAEPSLHA
                     VSEGMPVQEVLLDELPSEEPVKPAASKTAADILAERMKNLPHEPRQAAGSASRPESVA
                     VAKARTDVQRDAEEARYEQTNLSPDYTFDTLVEGKGNRLAAAAAQAIAESPGQSYNPF
                     FLYGSTGLGKTHLVQAVGNELLKNRPDAKVRYMHSDDYIRSFMKAVRNNTYDVFKQQY
                     KQYDLLIIDDIQFIKGKDRTMEEFFYLYNHFHNEKKQLILTCDVLPAKIEGMDDRLKS
                     RFSWGLTLELEPPELEMRIAILQKKAEAAGISIEDEAALFIANLIRSNVRELEGAFNR
                     VGASSRFMNRPVIDIDLARTALQDIIAEKHKVITADIIIDAVAKYYRIKISDVLGKKR
                     TRNIARPRQVAMSLTKELTTLSLPSIGDSFGGRDHTTVMHGIRAVAKLREEDPELAQD
                     YEKLLILIQN"
     gene            17025..18128
                     /locus_tag="WGQ68_000612"
     CDS             17025..18128
                     /locus_tag="WGQ68_000612"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEZ0714464.1"
                     /translation="MLILQAERDSLLKPLQAVTGIVERRHTLPILSNVLIEGKGGQTK
                     LLATDLEIQIDTAGPEGGAGDFRITTNAKKFQDILRALPAGALVSLDWDDSRLTLKAG
                     KSRFALQTLPAADFPMMNVGEDISATFSLEQERFKTMLSQVQYSMAVQDIRYYLNGLL
                     MQVEGSQLRLVATDGHRLAYAACAIDADLPHAEVILPRKTVLELFKLLNNPDDPIQIE
                     LLDKQVRFQCNGTTIVSKVIDGKFPDFNRVIPLDNDKIFVLSRAELLGALERVSILAN
                     EKFRGARLFLQPGLLSVVCSNNEQEEAREEIEIAYQGGELEVGFNIGYLMDVLRNIHS
                     DDMQLAFGDANRSTLFTVPNNPNFKYIVMPMRI"
     gene            complement(18368..18907)
                     /locus_tag="WGQ68_000613"
                     /pseudo
     CDS             complement(18368..18907)
                     /locus_tag="WGQ68_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611454.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(19140..21197)
                     /gene="ppk1"
                     /locus_tag="WGQ68_000614"
     CDS             complement(19140..21197)
                     /gene="ppk1"
                     /locus_tag="WGQ68_000614"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEZ0714465.1"
                     /translation="MPEQNRILCRELSLLAFNRRVLAQAEDKNVPLLERLRFLCIVSS
                     NLDEFFEVRMAWLKRENKLHPRRRLDNGKMPSETIADVTEAARSLIRHQYDLFNNVLQ
                     PELAQEGIHFYRRRNWTGAQKKWIESYFDRELLPILTPIGLDPSHPFPRPLNKSLNFA
                     VELDGTDAFGRPSGMAIVQAPRILPRVVPLPSELCGGGHGFVFLSSILHAHVGKLFPG
                     MNVKGCHQFRLTRDSDLTVDEEDLQNLRAAIQNELHDREYGDGVRLEVADTCPAYIRD
                     FLLAQFKLTAAELYQVKGPVNLVRLNAVPDLVNRPDLKFPTHTPGRLKALGKTASIFD
                     LVRQSPILLHHPYQSFDPVVEMMREAAADPDVLAVKMTIYRTGTRSELVRALMKAALA
                     GKQVTVVVELMARFDEANNVNWAKQLEEAGAHVVYGVFGYKVHAKMALVIRREDGVLK
                     RYAHLGTGNYHQGTSRIYTDFGIITADEQITADVNTLFMEITGLGKPGRLNKLYQSPF
                     TLHKMVIDRIARETEHAKAGKPARITAKMNSLIEPTVIEALYRASAAGVQIDLIVRGM
                     CTLRPGVKGLSENIRVRSIIGRQLEHARVYCFHNNGADDTFISSADWMGRNFFRRIET
                     AAPITAPELKKRVIREGLEMALADNTHAWLMQPDGGYIRAAPAEGESEADLQNDLWTL
                     LGG"
     gene            complement(21366..21809)
                     /locus_tag="WGQ68_000615"
     CDS             complement(21366..21809)
                     /locus_tag="WGQ68_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HEZ0714466.1"
                     /translation="MPLRLLLGILCDLSMVAPVYAQGQPDAVNAYIQKKKVIVDTSKA
                     ELCFADDRQCHPVLIGTATPKGTFGLTLNSTDKPGYGGEVIGFKQEGDFLFALHRVWN
                     QIPSERRNERIASPSVSDRIMTNGCINVSDAVYEKLRHYFVLEVI"
     gene            complement(21816..22331)
                     /locus_tag="WGQ68_000616"
     CDS             complement(21816..22331)
                     /locus_tag="WGQ68_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0714467.1"
                     /translation="MKIKQIVKPGLAVLAAGILSACATKSNVKADGTTDNPVFPKPYS
                     VTLDNKRGTFPTYDELDLMRPGLTKDDIYKILGRPHYDEGMYGVREWDYLFHFHTPGV
                     GTDPENTSGVEDVTTCQYKVIFDKDKFARSFYWNPVFPKDAACPPPAPKAEPQVIIRE
                     IVPAKPKRIRQ"
     gene            22604..25234
                     /locus_tag="WGQ68_000617"
     CDS             22604..25234
                     /locus_tag="WGQ68_000617"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEZ0714468.1"
                     /translation="MQEQYRPAAIEPAAQKKWDDARIFNVSEDASKPKYYCLSMFPYP
                     SGKLHMGHVRNYTIGDVLSRFKRLNGFNVMQPMGWDAFGMPAENAAMKNNVAPAAWTY
                     DNIEYMKTQLKSLGFAIDWARETATCKPEYYRWEQWLFTKLFEKGIVYRKNGTVNWDP
                     VDQTVLANEQVIDGRGWRSGALIEKREIPMYYFKITDYAEELLNDLDKLEHWPEQVKT
                     MQRNWIGKSRGMTVRFAVSDDSKQGLEGDYARFLQVYTTRPDTLMGATYVAVAAEHPL
                     ATAAAADKPELQAFIAECKAGSVAEADMATMEKKGVPTGRYVVNPLNGDKLEVWIANY
                     VLWGYGDGAVMAVPAHDERDFEFATKYSLPKKQVIAVGDNAFDANQWQEWYGDKENGV
                     LVNSGDLDGMNFQTAFDAIAAKLQSQGAGEPKTQYRLRDWGISRQRYWGCPIPIVHCE
                     QCGDVPVPADQLPVVLPENVVPDGMGSPLAKMPEFYETACPCCGGAAKRETDTMDTFM
                     ESSWYFFRYMSPKFSDGMVSAEAAKYWGAVDQYIGGIEHAILHLLYARFFTKLMRDEG
                     LVNVDEPFERLLTQGMVVCETYYRENDKGGKDWINPADVELTFDDKGRPVSAVLKADG
                     LPVVISGTEKMSKSKNNGVDPQELINAYGADTARLFMMFAAPPEQSLEWSDSGVEGAH
                     RFLRRLWRTVYEYLKQGGAVKAFAGNQDGLSKELKDLRHKLHSTIAKVSDDYGRRQQF
                     NTAIAAVMELLNQYDKTDTGSEQGRAVAQEVLETAVRLLWPIVPHICETLWSELNGAK
                     LWEAGWPAVDEAALVKSEIEVMVQVNGKLRGKITVAADASKADLEAAALATEGAVKFM
                     EGKPAKKIIVVPGRLVNIVV"
     gene            25462..26253
                     /locus_tag="WGQ68_000618"
     CDS             25462..26253
                     /locus_tag="WGQ68_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003761076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HEZ0714469.1"
                     /translation="MNLFFDTELGKQQNKATHKIRVMSEAWLEKNGYCPCCGSKPMKR
                     FANNKPVADLFCPNCHEQYELKSKNQKTIGNIVPDGAYHTMLERIRSDTNPNFFFLAY
                     KKADYSIRQLVLVPKHFITPDMIIPRNKGIKNRPHHIMCSINLAPLPESGKIFLIDDS
                     RIIEPETVLKKWQSNLFLRNQNAERKGWLLAVMKCIDQLPEEFTLSQMYEFENKLSIQ
                     FPQNNHIKDKIRQQLQILRDQNTIEFIGRGLYKKIDKLRPTSKAV"
     gene            26383..26514
                     /locus_tag="WGQ68_000619"
     CDS             26383..26514
                     /locus_tag="WGQ68_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl-tRNA synthetase"
                     /protein_id="HEZ0714470.1"
                     /translation="MRNFDKLAEEAAEHAAEGAVKFIEGKPAKKIIAVPGRLANIVV"
     gene            26537..27469
                     /locus_tag="WGQ68_000620"
     CDS             26537..27469
                     /locus_tag="WGQ68_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0714471.1"
                     /translation="MLRCRPKSVLDSDGIFLKFNFLRSKPKYEISFLPSFLPSFKRIL
                     CLSAVISVLGACTVVADVYGQDSATMNAAAAEDYMKTVELNKSAGNVDTTSKTARRVQ
                     AVFRRMLPYADAANNTGHKFDWKMTVFKNDELNAWAMPGGKMAFYTGIVDKLKLTDDE
                     IAAIMGHEMTHALHEHGKNKVGQQILTNTAAQIGTQIILDKKPDTNPELVGLGMDILG
                     TYGITLPYSRSLEEEADEGGMMLMAQAGYHPAAAVRVWEKMNQENDQNGFIYAITSTH
                     PTNNARIENLKRLLPTVMPVYEQSVRNKGRVNPL"
     gene            27695..27898
                     /locus_tag="WGQ68_000621"
     CDS             27695..27898
                     /locus_tag="WGQ68_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714472.1"
                     /translation="MLSGYAVCTAAVSAWFDAVGHLCGYALIFGSAVLPVSSGIFSSA
                     SIVNLLILPFAKSSMQWLRCPCF"
     gene            complement(27867..28178)
                     /locus_tag="WGQ68_000622"
     CDS             complement(27867..28178)
                     /locus_tag="WGQ68_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HEZ0714473.1"
                     /translation="MKYKSEALAAIHEMMEGAYNIGAIDKKTMRGFDKSCLTEIKPLS
                     GGDIKAIREKEALSQAAFAIYLNVGKNHVSAWERGVKKPSGAALKLLTIVKNKGIEAI
                     A"
     gene            complement(28175..28543)
                     /locus_tag="WGQ68_000623"
     CDS             complement(28175..28543)
                     /locus_tag="WGQ68_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEZ0714474.1"
                     /translation="MRIFKNQWIVKFAKKHKISDSELLEAVERADNGLIDADLGGGVI
                     KQRIARQGQGRSGGYRSLILFKQADRAFFVYAFAKNDRENISDKELDVYRKAAAYYLK
                     YTRAELAALKEDGIITEIES"
     gene            complement(29357..29442)
                     /locus_tag="WGQ68_000624"
     tRNA            complement(29357..29442)
                     /locus_tag="WGQ68_000624"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29406..29408),aa:Leu,seq:gag)
     gene            complement(29456..29806)
                     /gene="secG"
                     /locus_tag="WGQ68_000625"
     CDS             complement(29456..29806)
                     /gene="secG"
                     /locus_tag="WGQ68_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEZ0714475.1"
                     /translation="MEAFKTLIWIVNIISALAVIVLVLLQHGKGADAGATFGSGSGSA
                     QGVFGSAGNANFLSRSTAVAATFFFATCMAMVYIHTHTTKHGLDFSNVQQTQQAPKPV
                     SNTEPSAPVPQQQK"
     gene            complement(29813..30586)
                     /locus_tag="WGQ68_000626"
     CDS             complement(29813..30586)
                     /locus_tag="WGQ68_000626"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEZ0714476.1"
                     /translation="MYHQIGMWDQKWVIGNWKMNGRLQNNNSLMHRFRILPTAERVLI
                     GLAAPTVYLLQLHNAMQIVLNNRILTCAQDVSRFPDNGAYTGEVSAEMLADTGTDIVL
                     IGHSERSLYFGEKNEIQRRKMENVLNVGLIPLLCVGESLEEREAGKEHEVIAHQLSIL
                     QGLDTKNIAVAYEPVWAIGTGKVATVEQIADMHAFIYKEILSLCGSDVKIRVLYGGSV
                     KADNAADIFAVPYVDGALVGGASLSYDSFTAIISAAQNA"
     gene            30770..31273
                     /locus_tag="WGQ68_000627"
     CDS             30770..31273
                     /locus_tag="WGQ68_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEZ0714477.1"
                     /translation="MHALHFSASDKAALYREVLPQIESVVADETDWVANLANTAAVLK
                     EAFGWFWVGFYLVDTRSDELVLAPFQGPLACTRIPFGRGVCGQAWAKGGTVVVGDVDA
                     HPDHIACSSLSRSEIVVPLFSDGRCIGVLDADSEHLAQFDETDALYLGELAKILEKRF
                     EASRQAA"
     gene            31375..32031
                     /locus_tag="WGQ68_000628"
     CDS             31375..32031
                     /locus_tag="WGQ68_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEZ0714478.1"
                     /translation="MDFEKARFNMVEQQIRPWDVLDFDVLDALAEIPRELFVDEDLQG
                     LAYADMALPLANGHKMLEPKVVARLAQGLKLTKNDTVLEIGTGSGYATALLAKLAGRV
                     VSDDIDAEQQNRAKAVLDGLGLDNIDYVQNNGLTELSAGAPFDAVYVGGAVNLVPEVL
                     KEQLKDGGRMVVIVGRKPVQRALLITRKGDVFEEKVLFDTLVAHLDDKDADPFGGFQF
                     "
     gene            32108..32431
                     /locus_tag="WGQ68_000629"
     CDS             32108..32431
                     /locus_tag="WGQ68_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEZ0714479.1"
                     /translation="MDIVQLGPAELKAWMDEGRMFCLLDVRTDEETAVCSLPNALHIP
                     MNLIPLRQNELPDDVPLVVYCHHGIRSLHTAMYLADAGFENLYNLQGGIDAWAVEIDG
                     NMMRY"
BASE COUNT         7045 a         7879 c         9570 g         8166 t
ORIGIN      
        1 aattaggaga tatttggggc gaaaatagac gaaaacctgt gtttgggttt cggctgtcgg
       61 gagggaaagg agttttgcaa aggtctcagc ttgtgcctga tccgcagggg cagggcgggg
      121 tgtggcgtgt gttcattttg cctgagaatg caaatgccgt ctgaaagggc ttcagacggc
      181 attgtcggta cggctgcccg tcagggcatc agcatgccgc cgttgacgtg cagcgtttga
      241 ccggtaatgt attttgcctg atcggaagcc aagaacaaga ctgcatcggc aatgtcttgc
      301 gcgtcgccga atctgcccaa ggcggtttgg gcggtaaagg tttggcgggt ttcttccggc
      361 agggcgcggg tcatgtcggt atcgataaag ccgggggcga cgcagttgac ggtaatgccc
      421 cggctgccga cttcgcgcgc catggatttg gagaaaccga ttaagcctgc ttttgccgcg
      481 gcatagttgg tttgaccggc attgcccatc acgccgacga cggatgtgat gttgatgata
      541 cggccggcgc gctgcttcat catgccgcgc aagacggctt tggaggcgcg aaagacggat
      601 ttgaggttga cctgcatgat gtcgtcccac tcttcttctt tcatacgcat caggaggttg
      661 tcgcgggtga tgccggcgtt gttgaccaga atatcgagtt tgccgaacgt tttttcgatg
      721 tcggcaatca ggttttcgac ggtttcgggt tcggcggaat ttaatacgcg gccttcgccg
      781 ccccattgcg ccaaccgctc gctaatcgcc gccgcaccgc tctcactggt cgccgtaccg
      841 atgactttgg caccggctgc cgccagtgtg tcggcaattg ctgcaccgat accgcgcgat
      901 gcgccggtta ccaaagcgat tttgccgctt aaatcttgtg tgctcatatt ttttcctttt
      961 ttccgataat cgaaagactg cgccggacgg tgcagccctg tttgctggtt attcccactc
     1021 gatggtggca ggcggtttgc cgctgacatc gtacaccacg cggttgatgc ctttgacttc
     1081 gttgatgatg cggttggaca cgcgtccgag cagtgagtaa ggcaactcag cccaatgtgc
     1141 ggtcatgaag tcgctggtga tgactgcgcg cagtgcgacg acgtagtcgt aagtgcgtcc
     1201 gtcgcccatt acgccgacgg atttcacagg caggaatacg gcaaatgcct ggctggtcag
     1261 gtcgtaccaa gatgtgccgt tttcgtcggt ggtgttgcgt aattcttgga tgaaaatatc
     1321 gtctgcttga cgcaataagt cggcgtattc ttttttcact tcgcccaaga tgcgcacgcc
     1381 caaaccgggg ccggggaacg gatggcggta caccatttcg cgcggcagac ccaaagcaac
     1441 gcccagttcg cgcacttcgt ctttaaacaa gtcgcgcaac ggctcaagca gcttgagctt
     1501 catgttttca ggcaggccgc cgacattgtg gtgggatttg atggcgtggg cttttttggt
     1561 tttcgcgcct gcggattcga ttacgtcggg gtaaatcgtg ccttgcgcca gccatttggc
     1621 gttggtgagt tttttctctt cggcatcaaa tacttcgata aattccgcgc cgatgatttt
     1681 gcgttttttc tcggggtcgg tcacgccggc gagtttcgcc ataaactgcc cttcggcatc
     1741 gacgtgtatc actttcacgc ccaagttgcg ggcgaacata tccatcacca ttttgccttc
     1801 gttcaggcgc aagagcccgt gatcgacgaa cacgcaggtc agttggtcgc cgatggcgcg
     1861 gtggatcagc gcggcggcta cggaagagtc cacgccgccg gacaagccta aaatcacttc
     1921 gtcgctgccg acttgttcgc ggattttggc aacggcttct tcgatgtagt tcggcattgt
     1981 ccagcccggt tgtgcgccgc aaatatccaa aacaaagcgg ttcaacaggg cgcggccttg
     2041 tttggtgtgg gttacttcgg ggtggaattg gatgccgtag aattgttttt cggcgttttc
     2101 catcatggca atcgggcagg acggcgtgtc gccgatgacg gtgaaaccgg taggcagttt
     2161 ggaaacttta tcgccgtggc tcatccatac gtcgagcgtg tttggcgtat cgtcttgaat
     2221 gccgcgtgtc agctcgctgt cgatggtttt gacttgcgcg taaccgaatt cgcgctggtt
     2281 gccgggttgc acttcaccat ccaagtggtg cgccataaat tgcatgccgt agcaaatgcc
     2341 caaaatcgga atgcccaagt caaagatacc ggtatcggct tgatagtcgg attcgtaaac
     2401 ggaattaggg ccgccagaga ggatgatgcc tttggggttg aaggctttga tttcgtccaa
     2461 aggcatatcg aaggaatgca gttcgcagta aacgtgggct tcgcgcacgc ggcgggcaat
     2521 cagctgggta acttgagaac cgaagtcgag gatgaggatt ttgtcttggg tcataatggg
     2581 ttttacagtg attgaagata atcggatggg tcggcggata tgatgccgtc tgaaagggtc
     2641 ggggcgattg taccatttcg gacggcattt tgtttatgcc gtccggacgg cggcatcttt
     2701 gtttgagata ttgcgtaaca ttgtgtagta gccgttttgg gacatcagtt gttcgtgtgt
     2761 gccttgttcg atgattttgc cgtcgtccat tacgatgatg cggtcggccc cttcgatggt
     2821 ggtcaggcgg tgggcgacga tgatgccggt gcggttttcc atcaggcgtt cgagcgcctg
     2881 ttggacgagg cgttcggatt cgttgtctaa tgcgctggtg gcttcgtcca ataataatat
     2941 cggcgcgtct ttcaaaatgg cgcgggcaat ggcaacgcgt tgccgctgtc cgccggataa
     3001 gttgctgccg ttcgatccga tgggctggtg cagtccgagc ggtgatgcgt cgatcaggct
     3061 ttgcaggtta gcggcttgga gggcggatag gacttcggct tcgcccgcgt cgggacggct
     3121 atatcggacg ttttcaaaca gggtgtcgtc aaacaggaat acgtcttggg agacgagggc
     3181 aaattgggcg cgcaggcagt cgagtttgat gtcggcgatg tcgataccgt ctatgcagat
     3241 gttgccggca gacggttcga caaagcgggg cagcaggttg acgacggtgg atttgccgct
     3301 gccggaacgt ccgaccaagg cgacgcgttc gccttgtctg atgtcgagat tgaagttgtc
     3361 gagggctttg atgccgtctg aacggtattc gacatcgacg ttgcggaagc tgatgcgccc
     3421 ttcgacacgc tgcggtgtga gcgtgccttt gtcctgttcg ggcggggtgt cgagaaatgc
     3481 acatacgccg tcggcggcga ggaacatcgt ctgcataggg atgctgatgt tggcaaggct
     3541 tttgatgggg gcgtacattt gcagcatcgc gacgatgaat gccataaatt cgccgatggt
     3601 ggtgtagcca ttttggcttt gccacagggc gatgaagatg acgacggcga gggcgatcga
     3661 ggcaatcagt tcgctgaacg gggaatgtgc cgccgttgcc tgcgtgattt ttttgctgag
     3721 gcggacgatg gtgcggttga tcgcgtcgaa ccggtttgcc gcctgcgcct gcccgttgaa
     3781 cagcttgacg acgcggtgtc cctgatgggt ttcggcaatc acgttgttca tcgtgcctat
     3841 gcttttttgc gagtcggaaa tgacgtgttt cagacggtcg cggtagtagc gcgagagcag
     3901 ggagagcagg gggaacatca ggacgacgat aatgctgagc tgccagttga ggtaaagcag
     3961 gacgatggtc aggccagtaa caatcatcgt gtcgcgcgtg aggacggtga agatgtcgct
     4021 ggcgttactg accgactgtt cggtcaggtt gagcatattc atcagtacgg tgccggacgg
     4081 cgtttcctga tggtagcggg aggaaagggt cagcattttg gcaaacatat ctttgcggat
     4141 tttgctgatg gtcatcacgg agacccaagt catcagatag gtgctggtaa agcggcagat
     4201 gccacggatg acgacgagga tgatgaggaa gagcgggacg gtccagattt tgttttccgt
     4261 cccccaaacc atataggtaa actgttcgcg ccagttttgc agggtggaaa tgatgccggc
     4321 ggcggcagac agctcgggcg gcgcggcagg tgcggaaaag ccgtggttaa tcaggggggc
     4381 gatgaaggcg gcaaggtagc tttcggtggc ggcaacgccg aaaatggcaa tcagggcggc
     4441 aacgatgcgg attttgtagg ggcggacgta tgccatcagg cgcataaagc tgcgcgcgtc
     4501 ttctttttta aacagtccga aagtcagttt ttctatcata tcggtagggc gggaactgca
     4561 acggcagccg ctgtcggtgg tttggtcggg atggattata gcgtaaatac ggggctgccg
     4621 ccgttcggac gtgaaaagca gcctgaagag ggtaggctgc tttttacaga acgaagtcag
     4681 tgcgcttcga taaaggcggc aatctgtccg gcatcggtca gcgcgctgca cgcggcggtt
     4741 ttgttgatgc gttttgccaa gcccgccaac actttgcccg gtccgcattc ggcggattcg
     4801 gcaatgccgt ctgaaacaag ggcgttgacg gtttccgtcc agcgtacggg gctgtaaagc
     4861 tggcggacga gcgcgtcttt gattttgtcg gcatcatcgt aggcggcaac gtcggcgttg
     4921 tggatgacgc ggatttgcgg ctgcttgatt tcgacggttt tgagggcttc ggcgagtttg
     4981 tcggcggcgg gtttcatgag gctgcaatgg gaaggtacgg acacgggcag cggcagggcg
     5041 cgtttggcac cggcttcttt ggcggcggtc atggcgcgtc cgacggcggc ggcattgcct
     5101 gcaattacga cttgtccggg cgagttgaag ttgacggctt caaccacttc atcctgtgcg
     5161 gattcggtgc aaatctgcct tacctgttca tcttccaaac ccaaaattgc cgccattgcg
     5221 cccacgcctt gcggtacggc ggactgcatc agttcggcgc gtaggcgcac gagtttgacc
     5281 gcgtcggcaa aattcaatgc gccggcggca acgagtgcgg tgtattcgcc gaggctgtgt
     5341 ccggcaacgg cggcaggcgt tttgccgccc gcttccaaat aggcgcggta aacggcaacg
     5401 ccggcggcga gcataatggg ctgggtgttg acggtttgac cgatgatttc ggcatcgctg
     5461 ccgttaatca tcgcccacaa gtcctgcccc aatatggcgg aagcttcgtc aaatgtggct
     5521 ttaacgatgg cttgctcggc aaagccgttc atcatgccta ggctttgtga gccttgtccg
     5581 ggaaagaaga atgcaaaaga catagtattc ctttgtttgg ttggataatc aggtattctg
     5641 cccgagtttt acgaaaaatt caggcgggac aaaataatat agtggattaa caaaaatcag
     5701 gacaaggcga cgaagccgca gacagtacaa atagtacggc aaggcgaggc aacgccgtac
     5761 tggtttaaat ttaatccact ataaatcagg atgagggtcg ccaggacatt gcccgaaata
     5821 gtcagacgga acagcacaac aaaacgcttt ttcgccgttt tatgcctgaa tatcctgctg
     5881 cccaagtatg cgcccgccca accgccgaac aaggcaggca gcagcaggcg gtgttcggga
     5941 atgcggcgtt ttccccgcac ggcacgccgt ttgtcgatgc cgtaaagtgc aaacgcatag
     6001 agggacaata ttgcgtaaca cgcgcccaat acggggaggc gcagcgaaac ggcagacaga
     6061 aatgccgcac acgccatcag tttgaagaaa gcctgccgct tcatgatatg ggaaatgccg
     6121 tctgaaagcc tgtttcagac ggcatcgggt tcaatattgc aacagcaccg cgccccacgc
     6181 gaaaccgccg ccgatgcctt cgagcagcag gttttgaccg cgtttgattt gtccgctgcg
     6241 gatgcccgta tccaaagcca gcggaatcga tgcggcggat gtgttgccgt ggtcttggac
     6301 ggtcaggacg actttgtcca tactcaaacc taaatgtttc gcggtcgatt cgataatgcg
     6361 gcggtttgcc tgatgcggca cgatccagtc gatttgagcg gcggtataac cggcttcttc
     6421 gataacgtca tcggcgattt tggacagcat tttgacggca aacttgaaca cgccgggacc
     6481 gtccatcgaa atgtacggcg aaccggaaac tttgccgcag gcgatttgcc cggggacgtt
     6541 taagagtttc agataattgc cgtcggcctt gagtttgctg tggatgatgc ccggcgtgtc
     6601 cgacgcgctt aaaaccaccg cgcccgcgcc gtcgccgaac aatacgcagg ttgtgcggtc
     6661 gttccaatcg acgatgcggc tgaaggtttc cgcgccgatg accagcgcgt ttttcgccat
     6721 gccactttta atgtaggcgt ttgccgtggt cagcgcgtac ataaagccgg cgcacaccgc
     6781 ctgtacgtca aacgcgggac agccgttggt gatgcccaat ttttgctgca cgatggtggc
     6841 ggtagaagga aactgcatat ccggcgttgc cgtcgccacg ataatcaaat cgatttcgcc
     6901 gctgtctaat ccggctgcat ccagcgcgcg gcgggctgct tcggcggcaa gatcgctggt
     6961 tttctcattt tcggctgcaa tatggcggaa cttgatgccc gtgcgcgcgg taatccactc
     7021 gtcagaggta tctacctttt gggcaaggtc gtcattgctg acgcggttgg caggaagata
     7081 gctgcccgtg ccggaaattt ttgcatactg catgggaacg gcctttttag agagttggtt
     7141 cagattcggc tattgtaagc gaaaaaccga tgattgccca atacggacag ggtgcaaatg
     7201 acggaaacag ggatgggggt gtggtttggg atacagatgg ttttcagatg cagattgccg
     7261 tggataggca gggcggggtt gccgatacgc tgttttaagc gaaggaaatg ccgtctgaaa
     7321 accaaacatg cttcagacgg cattttgatt gttggttttc catcaaatta ttggtgccgg
     7381 tgtttgtttt tgtatcataa attcagaaat tatattttga attgtagaaa tatttttaga
     7441 gatatgctca atagggtaga agtctcccca aatttccatt gtatttccac tataattttc
     7501 tgttttaagg tttttgactt caatatcccc atcttctaaa attgtttgaa tcattggcaa
     7561 ataacatcct tcttcaaaat agatagttaa ttcggtattg ccaaatttgg gttcaggaat
     7621 aattctgaaa acaaggacgc cggctttttt ctcaaaattt gatacggaaa aaaagtgatt
     7681 gatttcccta atgccaggat tttccaaaac ggggatttgg attcgctccc tgttgtctgc
     7741 cataaaaaat ccgccaaaaa cgaccgtcga tgttttcatt cttatcaaat cattggcttt
     7801 attggttaat cggacgtgct ttctatctca aattccttgt tgataaaaga aatttcccgg
     7861 gattcttgtt ccgtcagccg gacatcgaaa tcttcgatat aagtacacag gatttcaaaa
     7921 gccaaaaaac gttccgaata atgaatataa tccaatgcgg attcaatcag acgggcatca
     7981 agcctgcctt caagggattt tccgaacttc atcatctgtt tgtctagagc tttcatccgt
     8041 ttttctaatg tcttcatttt tcaaatactc cttattttac agggaagtat ttgggaagtg
     8101 ccggacaggc gcatcgccgt ccgcgcccgc aggtttggtg cgggcggatg ccgtctgaaa
     8161 ggggcggggg cgttcagacg gcatcgtgtg tgttacagac cgtcggcatt ttcgttttgg
     8221 acggctttgg cagtttcgag tgcggcgagt tgttcggcaa cgccttgttc gattttggaa
     8281 aggccggcgg acttggcttc gtggtaggct tcttcgagtg cgtagcggaa accggtttcg
     8341 tctgtgccgc cgtggctttt gatgacgatg ccgcgcagcc cgagcaggat ggccccgttg
     8401 aatttgcgcg gatcgagttt gtttttcagc cctttgaggg cgggtagggc ggcaacggcg
     8461 gcaagtttgt tgaacaggtt gctttggaat tcgcggcgga tggctccgct catgaatttg
     8521 accgcgcctt cgatggtttt gagcatgacg ttgccgacaa agccgtcggc gacgacgaca
     8581 tctgcttcgc cgtagaggat gccgttgctt tcgatgttgc cgataaagtt gagtttgctg
     8641 ttttgcagca gtttgtaggt ttgtttgacg gtgtccgtac ctttgatgtc ttccgtgccg
     8701 acgttgacca gcccgacgcg cggctgtcct ttttgaggat ggagtgcgtg gacgagttcg
     8761 ctgccgataa cggcaaattg ggcgagctgt tcgggcgtgc agtcgacgtt cgcgccaagg
     8821 tcgagggcga gggtaacatg gtcggtgtcg gacgggagga atttggcgat ggcggggcgt
     8881 tcgataccgg gtatggtctt gaggacgaaa cgtgcggttg ccatgagtgc gcccgtgttg
     8941 cctgcggata cggcggcttg ggctttgcct tctttaactt ggttgatggc aacgcgcatg
     9001 gaggagtctt ttttgttttt cagggcggat tgcggcgctt cgtccatgcc gacgatttgt
     9061 gcggtgtggc agatgtcgat gcgttccatc ggtgcgcctg ccgcggtcag ggcttggcgc
     9121 agttgcgttt cgtcgccggt cataatcagg cggacatcgg ggtgtgcttg gaggaatgcg
     9181 gttgcgccgg gtacggtaac ggcaagtcct tggtcgccgc ccatggcatc tacggcaagt
     9241 gtaatcatag ggttctccgg taggttttcg gtctgcggca ggggtcgtgc gggcttcaga
     9301 cggcatcggt tcggaaaata tgccgtctga aagggtcagc ctcggccggc ctcggcaagg
     9361 cggttgtgtt cgtcgatgtc gagtgcgtcc catggatagt tgatccacca gtcttctacg
     9421 gtgatgccgc tgaaataggg aatgccttcg gggattttac ctgctttggc tttgattttt
     9481 tcgtgcagta cggcgacgcc gatggtgccg aagtcttctt taagcagctc tgtcaggcaa
     9541 aattccatgg ttacgcggct gtcgtccact tcatcgacaa caaggacgtt tttgcctcgc
     9601 agggcttcgg gaacggggtc gagccattgg acttttttga cttcttctgt aacctgtcct
     9661 tcgttgtcgc tgtcgtaata ggcggtggtt acggcataaa tcggaatttc cagaaaacag
     9721 cgcagcatac gtgccggaat aaagccgccg ccgccgatgg caatcatggc atcgtatttg
     9781 atgtcggcgt tccggatttt ttctgccaat gctttgatga cgcggtggat gtcatcgtag
     9841 gtgtaccaga ttttctgttt catcggaatt tccttggtgg ttggggcttt tggaatattt
     9901 tcacggggta ttatacggaa cgctttgccc ctgtgtgttg acgtatggcg aaagtttgga
     9961 cggatggtgc gtccaatgca aaaaaagcca gtaattgcat ggcaatgttc tggctttttc
    10021 agtcagtcga atagggatta ttcgcctttg gctttgacca ctttgcggcc gcggtacata
    10081 ccgttggggg agatgtggtg cgggcggtgt acttcgccgg ttgtgctgtc gacagacagt
    10141 gcaggcgcgg tcagcgcgtc gtgcgaacgg tgcataccgc gtttggaagg ggattttttg
    10201 ttttgttgaa cggccatttc aagctcctag ataaataaac tgtgtcctaa ttaactgctt
    10261 ttcaaacctg ccaaaacagc aaaggggttg ggtttgtccc gattgacttc ttccagtgtc
    10321 ccattgtcgc cgcagtcttc gtgtcgcggc gaaaagggca gggacatcag gatttggtct
    10381 tctaccaatg tgcgcacgtc gagttctttt tcaatcagta tgccttcgag ttcttcgtcg
    10441 gcaagcatgg attcgtccaa ggactcttcg ttggaaaaca ggacgatacg gctgctttca
    10501 tcaagcatga acggcatggg tttgatacat ctctggcaaa tcaggggcat atcggctttg
    10561 acgttcaggt cgaggaacag gcgttgcagc cgatcgcgac cgccggtcag tgtaaacgat
    10621 attttggtct gcctgtcggc gggataatcg tgcgaactga cgcgttcatc caattcttcc
    10681 agcagaaaac tgccttgcag gttctgccct tcggcggcaa aaatttccgg gtcaatcaaa
    10741 ttagggtctg acataaacga ggtatgatat aatttagatg ttctaacgtc aatattttta
    10801 agaaaaatgt tgcggtacgc cgaaggcggg tatccgtgtt tcggcgcatg gctggatttt
    10861 accgcccctt cgggcgtgtt ttcaacggta aggaggatgg gatgggtttg gaactgcctt
    10921 tgattttggg tacgagttcg gttttccgcc gcgaacagat ggaaaggctc ggcattgcct
    10981 ttcaggcggc atcccccgat tttgacgaaa cgccgatgtt gggggaatcc gcccctcaga
    11041 cggcattgcg ccttgccgag ggtaaggcgc ggtcgttggc cggacgtttc cccgaggcgt
    11101 tgattgtcgg tgcggaccag gtggcgtggt gcgacggcag gcagtggggc aagccgatga
    11161 accttgccaa cgcgcaaaag atgctgatgc atttgagcgg cagggagatt gagttttaca
    11221 gcgcggtcgt tctgctaaat acggttacgg gcaggatgca gcggcatatt gataagacgg
    11281 tggtcgtgat gaggcagttg gacgagttgc acatcctccg ctatttggag cgcgagcctg
    11341 atgcggttta ttgttcgtgt gcgttgaaga gtgaggattt gggtgcattg ttgatcgaaa
    11401 ggattgagag taccgatccg aatgctttaa tcggtttgcc ggttttccgt ctggttgatt
    11461 tcttgaaaaa tgagggcgtg gaggttctgt aactgcccgt ttatgccgtc tgaaaggctc
    11521 agacggcatt tttaaccgat gaatgaggga atgatgtctc ctgttttgta tttgattcct
    11581 acgcctttgg gtacgcctga cacgccgtgc ctgttgccgc atgaacaaca ggcgattgtc
    11641 gggctgacgg attttgtcgt ggaagcggaa aaaacggcgc gcgcgcattt gaaacatttg
    11701 ggcgtgacaa tgcctatccg cgaactgaat ttgcaaacgc tgaacgaaca cacggatttg
    11761 aagactttgc tggaattgct gaaacctttg caagaagggc gcagtatggg cattgtcagc
    11821 gaggcgggtt gcccggctgt tgccgatccg ggtgcgaatt tggtggcatt ggcgcataaa
    11881 cacggttttg aagtacggcc tttggtcggt ccgtccagct tgttgctggc gctgatggct
    11941 tcgggtgcga atgggcagaa ctttgcgttt aaaggttatc tgccgtctga aaaaaacgag
    12001 cggattcaga gtttgaaggc tttggaacaa cgttcgcgcc agcagaatga aacgcagctg
    12061 tttatcgaaa cgccgtatcg caacgatgtg ttgttggcgg atgcggtgga aaacctgcat
    12121 tctgaaacgc gtttgtgtac ggctacggat ttgacgttgc cgactcagga aatcatcagc
    12181 cagacggttg tgcagtggcg aaaaagaaaa gaaatgccca atctgaaaaa acgcccgacg
    12241 atttttgtga tgtatgcagg ttgaagattt ccgccccgat agggggtgaa caataaaaat
    12301 gccgtctgaa caggtttcag acggcatttt tcatgctgcg gcatttagga aacgacttcg
    12361 ccttgggcgc gttgtttttc gatgctgcgg ttgatgtgcc attgctgggc gatggtcagg
    12421 aggttgttga ctacccagta caataccaga ccggcaggga agaagaagaa catgacggag
    12481 aaaaccaacg gcatgatttt catcattttc gcctgcatcg ggtcggtcgg cggcgggttc
    12541 agataagttt gggcgaacat cgttgccgcc ataatgatgg gcaggatgta gtaggggtcg
    12601 gcgcggctga ggtcggtaat ccaacccagc caaggtgcct ggcgcaattc tacggaggcg
    12661 aacaatgccc aatacaatcc gatgaagacg gggatttgca acagcatagg caggcagccg
    12721 cccagcgggt tgattttctc gtctgtgtaa agctgcatca tcgcctgttg ttgcgccata
    12781 cggtcgtcgc cgtatttctc tttgatggct tgcagtttgg gtgcggcggc acgcattttc
    12841 gccatagagc ggtaagaggc gttggtcaat ggatacagta cggctttgac gatgatggtt
    12901 aaaacgataa tcgcccagcc ccagttgccg atgatgttgt gcagttggtt caaaagccaa
    12961 aagagggggg aggcgaacca gtgtactttg ccgtagtctt tggccagttg caggttgtcg
    13021 gcgatgtttg cgatgacgga agtggtttgc gggccggcat acaggttgac ggcggtttcg
    13081 gctttcgcgc cggccgggat ggcggcaagg ggaacgctga cgcttgcgct gtacagcttg
    13141 tcgttgcggc gtttgatgtc gatgctgcag tcgccagcga cgcaaacgct ttgtccgcct
    13201 ttgggttgca aaatccaagc ggacataaag tggtgttcga tcataccgag ccagccggtc
    13261 ggggttttgc ggatgtattc ggcctcggat ttgccggatt tggcatcgtc gtccaagtcg
    13321 gagaagctga ctttttggaa gttgccttca ggggtataaa caacaggacc gacgtaagag
    13381 tgggtaaagt aaccttgacc ctcgggttcg ctgtggtcgc ggacgatgcg gtagtccgcg
    13441 ctcaggttgg cggtttgacc gctgccgttg gcgatgtcga agcggacgtt gaccagatag
    13501 ctgcctttgg tgaaagtata aactttgtcg attttcaggc cgttggtttc aggtgcgctc
    13561 aggcggactt caactttgtc gccttccaaa ctgtattgtt tttgcggagc ggtaaagccg
    13621 atgcctttca gaacgttgtt gccttgtgcg tccaaaagct cggattgggc gacgtaggtg
    13681 tattctttgc cgtcgccaaa caggacgaag ggtttgtgtt cgtcgccggt tgctttgtat
    13741 ttgagcaggg tcagccggcg caggtcgccg cttttttcat caatgacggc ttgaaccgtg
    13801 tcggtcgtta cggtaatcgg tgttgcgggc gcgagcgcgg cttcggcgga agcggttacg
    13861 gcctgttgtt gtgctgcctg ttggggcgcg gggacgggct tcggagtggg gaacatcttt
    13921 tcccatccga tcataatcac cagtgcgatg gcgaaaaacg ccgtgagtct tttaaaatcc
    13981 ataagagttt cctgaatggt cggaaatgcc gcaggttgcg tgttccgata gacggggaat
    14041 aagggcgggc ttcatacccg acggtcgtgc cgccagcccg aagtttcaga cggcattata
    14101 tagaaaccga tgggaaataa aaggaaagcg tgcaatttga atattgcgtc gggtcaggga
    14161 acggggtcgt gtccgtgtcc gccgaaaggg tggcagcgtg caatgcgctt gatggcgagc
    14221 cggccgcctt tgaatgcgcc gtattttttg accgcttcga ccgcgtattg cgaacaggtc
    14281 ggcgtatagc ggcagcgcgg cggaatcagc gggctgatgc aatattggta gaaccgtatc
    14341 agtcccagca ggagtttgga caataggaag ttcatcggga tgttccttgt atgggaaacc
    14401 cattgccgtc tgaaccttgc ctgcagagta ccgttctgat catacctgtt tcccgcatcc
    14461 ggttgcgggg ttgccgaaca tgagttgtgc cagttccgcc cttgcctgtt ttgcggtagc
    14521 cctgtcgaat ttacggcgga cgcgcacgac gaaatcctgc ggcggcagcc ggtttttgtt
    14581 caatctgaac cagtcgcgga taacgcgctt catataattt cgttcgttgg cgcgtttggc
    14641 ggtttttttg ccgaccacca gaccgatgcg gggatgatcc agcccgttgc cgtttgagcg
    14701 cgaaacttgc agcaggtcgc ggctgcggcg gtttctgaat gcaaaaacgg atgaaaaatc
    14761 atccgttttt aacaagcggt actgccttcc gaagcggtag tccaaaatta taccgccagg
    14821 cgtttgcggc ctttggcgcg gcgtgcagcc aatactgcgc ggccgccgcg tgttttggag
    14881 cgcaccagga agccgtgggt gcgtttgcgt ttggtaacgg aaggttgata agtgcgtttc
    14941 atgatgtttc ctaaaataga atgggtagat aattaaaccg tgaattacac tccaatttgc
    15001 cgcttttgtc aatcaatata gaaactttgc aggaagattg cccgattgcc tgcggatttc
    15061 cgccgccttt ctgtggataa atttttgcac gggttgtgga taaaatattc gtgagtcggt
    15121 ataatcgggc agtcgcgttt tgaaccgatg tgcgttcaac agatttgttt tctttttgaa
    15181 aatattatat tttctttgtt ttcgatttca tttttaccga ttcgagccta tccatgacat
    15241 tagcagagtt ttggccgctg tgcctccgcc gtcttcacga tatgttgcct caagggcagt
    15301 ttgcgcaatg gattgcgccc cttaccgttg gtgaggaggg tggcgtatgg gtggtgtacg
    15361 gcaagaacca gtttgcctgc aatatgctca agagccagtt tgccggaaaa atagaagcgg
    15421 tgagggaaga gttggctgcc ggccgttccg ccttcgtatt caaaccggga gaaggcgtgc
    15481 gttatgagat ggcggcggtt gaaggtgctg tcgaacctgc cgagccgtcc ttgcatgcgg
    15541 tgtcggaggg aatgcccgtg caggaggttc tgttggacga gctgccgtct gaagagcctg
    15601 tcaaacccgc tgcgtcgaaa acggcggcgg atattttggc ggaacgtatg aaaaacctgc
    15661 cgcacgagcc gcgtcaggct gccgggtctg cttcccggcc ggaatcggtg gcagttgcca
    15721 aagcacggac ggacgtgcag cgcgatgcgg aagaggcgcg ttacgagcag accaacctgt
    15781 ctccggatta cacgtttgat acgttggtag aaggtaaggg caaccgcctt gcggcggctg
    15841 cggcgcaggc gattgcggaa agcccggggc agagttacaa cccgttcttc ctctatggca
    15901 gtacgggttt gggcaaaacc caccttgtgc aggcggtcgg caacgagctg ctgaaaaacc
    15961 gtcccgatgc caaagtgcgc tatatgcatt cggatgacta tatccgcagc tttatgaagg
    16021 cggttcgcaa caacacttac gacgtgttca agcagcaata caagcaatac gacctgctga
    16081 ttatcgacga catccagttc atcaaaggca aagaccgtac gatggaagag tttttctatc
    16141 tgtacaacca tttccacaac gagaaaaaac agctcatcct cacttgcgat gtgttgcctg
    16201 cgaaaatcga aggcatggac gaccgcctca aatcccgttt ttcatgggga ctgactttgg
    16261 aactcgagcc gcccgaattg gaaatgcgca tcgccatttt gcagaaaaag gcggaagcgg
    16321 cgggcatcag tatcgaagac gaagccgcgc tgttcattgc caatctgatc cgttccaacg
    16381 tgcgcgagct tgaaggcgcg tttaatcgtg tcggagcgag cagccgcttt atgaaccgtc
    16441 ccgtcatcga catcgatttg gcgcgtaccg ctttgcagga cattattgcc gagaagcaca
    16501 aagtcatcac cgccgacatc atcatcgatg cggtggcgaa atattaccgc atcaaaatca
    16561 gcgacgtact cggcaaaaaa cgcacgcgca acattgcccg tccgcgccag gtggcgatga
    16621 gcctgaccaa agaactgact actttgagcc tgccgtctat cggcgattcg tttggtgggc
    16681 gcgaccatac gaccgttatg cacggcatca gggcagtggc gaaactgcgc gaagaagatc
    16741 cggaattggc tcaggattat gaaaagctgc tgattctgat tcaaaactga tggaaaacgt
    16801 tttcagacga catgatattg cccatgccgt ccgaagggta aagatatagc ggattaactt
    16861 taaaccagta cggcgttgcc tcgccttagc tcaaagagaa cgattctcta aggtgctgaa
    16921 gcaccaagtg aatcggttcc gtactatttg taccgtctgc ggcttcgtcg ccttgtcctg
    16981 atttaaattt aatccactat acaaaccgat ttaaaggagc gaacatgttg attttacaag
    17041 ccgagcgcga cagcctgctc aagccgttgc aagccgttac cggcattgtc gaacgccgcc
    17101 acaccctgcc catcctgtcc aacgtcctga ttgagggaaa aggcggtcag accaaactct
    17161 tggcaaccga tttggaaatc caaatcgaca ctgcgggtcc cgagggcggc gcgggcgact
    17221 tccgcatcac taccaatgcc aagaaatttc aggacatttt gcgcgcgctg cctgccggtg
    17281 cgctggtgtc gctggattgg gacgacagcc gtctgacgct gaaggcgggc aaatcgcgtt
    17341 ttgccctgca gaccctgcct gccgccgatt ttccgatgat gaatgtcggc gaggacatca
    17401 gcgcgacttt ctcgctggag caggagcgtt tcaaaaccat gctgtcgcaa gtgcagtaca
    17461 gcatggcggt gcaggacatc cgctattatc tcaacggtct gctgatgcag gttgagggca
    17521 gccagttgcg ccttgtggcg accgacggac accgccttgc ctatgcagcc tgcgcgattg
    17581 atgcggattt gccgcacgcc gaagtgattt tgccgcgcaa aacggtgctg gaactgttca
    17641 aactgttgaa caaccccgac gatccgattc aaatcgagct gctggacaaa caggtgcgtt
    17701 tccaatgcaa cggcacgacc atcgtcagca aggtcatcga cggcaaattc cccgatttca
    17761 accgcgtgat tcctttggac aacgacaaga ttttcgtttt gtcccgtgcc gaacttttgg
    17821 gtgcgctgga acgtgtgtcc attcttgcca acgaaaaatt ccgtggcgcg cgcctgttcc
    17881 tgcaacccgg cctgttgagc gttgtgtgca gcaacaacga gcaggaagaa gcgcgcgaag
    17941 aaatcgaaat cgcctatcag ggcggcgaac tcgaagtcgg tttcaatatc ggctatctga
    18001 tggacgtgtt gcgcaacatc cattccgacg atatgcagct tgccttcggc gatgccaacc
    18061 gctcgacgct gtttaccgtg ccgaacaatc cgaatttcaa atatattgtg atgccgatgc
    18121 ggatttgacg gttttccgga acacgatgcc cgtattggag atataccccg aaccgtgcag
    18181 acggattcgg ggttttgttc ggctgtcgga aaggtatagt ggattaacaa aaaccagtac
    18241 ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctgaag caccaagtga
    18301 atcggtttcg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa
    18361 tccactataa ttttcgcaag acgcgttttg cctgattcca aaaattctca atgccgttaa
    18421 tgtggttctg acggtctgca aattccttgg aatggttgat gcggtaatga atgaaatcgc
    18481 tcacgtccaa cttgtcgtag ctgctcagac tatcggtata aacaatgctg tccggcatga
    18541 ttttcttctt gatgacaggg agtaacgttt cagacttggc attgtttacg acaacggtat
    18601 agacccgtcc gttgcgtttc agaatgccga agacaaccac ttttcctgcc gcaccgcgac
    18661 cacgtctgcc tttacgccgt ccgccgaaat cgctttcgtc cggctcgaca gggccctcaa
    18721 aaacctcatc ggcagccaag gccaaatgat ggttgataac cgtgcggatt ttacggtaga
    18781 acagtgctgc cgaattggga tggataccca aaatatcggc ggcagaacgg gcggtaactt
    18841 ccagcacaaa aaaacggagc agttctttct gtactttttt ctttaatttg cagtgtgtta
    18901 tcttcatatt tcgagggtaa catatctgct aatctagtac agcccctaaa atttaatcca
    18961 ctataatgcc gtctggaatg cggcggattg gggttgggag cgtatagggg aagtgcttgt
    19021 gcggaaatgg cgaaggtgtg tttttgactc cggtccgccc tgtttagatt gctgtaaaac
    19081 catgccgtct gaaacggaaa ggtttcagac ggcatggttt ttgattgggg cggtgcgggt
    19141 cagcctccga gcagtgtcca caaatcgttt tgcaggtcgg cttcggattc gccctcggca
    19201 ggtgcggcgc ggatatagcc gccgtcgggc tgcatcagcc acgcgtgcgt attgtctgcc
    19261 aatgccattt ccaaaccttc gcggatgacg cgttttttga gttcgggcgc ggtaatcggc
    19321 gcggcggttt cgatgcggcg gaagaagttg cgccccatcc aatccgcgct ggagataaag
    19381 gtatcgtccg cgccgttgtt gtggaagcaa tacacgcgcg cgtgttcgag ctgcctgccg
    19441 atgatggagc ggacgcggat gttttcggac aagcctttta cacccgggcg caaggtgcac
    19501 ataccgcgca caatcaaatc gatttgtacg cctgccgcgc ttgcccgata cagggcttcg
    19561 atgacggtcg gttcgatgag cgaattcatc ttggcggtaa tccgcgccgg tttgccggct
    19621 tttgcgtgtt cggtttcgcg tgcgatgcgg tcgataacca ttttgtgcag ggtaaacggg
    19681 ctttggtaga gtttgttcag ccgcccgggt ttgcccaagc ctgtgatttc cataaacaat
    19741 gtgttcacat cggcggtgat ttgttcgtcg gcggtaatga tgccgaagtc ggtgtagatg
    19801 cgcgatgtgc cttggtggta gttacccgtg ccgaggtggg cgtaacgctt gagtacgccg
    19861 tcttcgcggc ggatgaccag tgccatcttg gcgtggactt tgtagccgaa cacgccgtac
    19921 acgacgtgcg cgcccgcctc ttcgagctgc ttcgcccagt tgacgttgtt ggcttcgtca
    19981 aaacgcgcca tcagttcgac gacgacggtt acttgtttgc cggcgagtgc cgccttcatc
    20041 agggcgcgga cgagttcgga acgcgtgccg gtgcggtaaa tcgtcatttt gacggcaagc
    20101 acgtcggggt cggcggcagc ttcgcgcatc atttcgacaa cggggtcgaa cgattggtag
    20161 gggtggtgca gcaggatggg cgattggcgc accaaatcga atatggacgc ggttttgccc
    20221 aaggctttca gtctgcccgg cgtgtgtgtg ggaaatttca aatcggggcg gttgactagg
    20281 tcggggacgg cgttgaggcg cacgaggttg accgggcctt tgacctgata gagttcggcg
    20341 gcggtcagtt tgaattgcgc gagcagaaag tcgcggatgt aggcgggaca ggtgtcggcg
    20401 acttcgagcc gcacgccgtc gccgtattcg cggtcgtgca gttcgttttg gatggcggcg
    20461 cggaggtttt gcaggtcttc ttcgtcaacg gtcaagtcgc tgtcgcgcgt caggcggaac
    20521 tggtggcagc ctttgacgtt catgcccggg aagagtttgc cgacgtgggc gtggaggatg
    20581 gacgacagga agacgaagcc gtgtccgccg ccacacagtt cggacggcag gggaacgacg
    20641 cgcggcagga tgcgcggtgc ctgcacaatc gccatccccg aaggcctgcc gaatgcgtcc
    20701 gtgccgtcga gttcgacggc gaagttgagc gatttgttca gcggtcgcgg gaaggggtgg
    20761 gacgggtcga gtccgatggg ggtcagaatc ggcagcaatt cgcggtcgaa atagctttcg
    20821 atccactttt tctgcgcgcc cgtccagttg cggcggcggt aaaaatggat gccttcttgt
    20881 gccagctcgg gctgaaggac gttgttgaac aggtcgtact ggtgccgtat cagggagcgc
    20941 gccgcttcgg taacgtcggc gatggtttca gacggcattt tgccgttgtc cagcctgcgc
    21001 cgggggtgca gcttgttttc acgcttgagc cacgccatgc ggacttcaaa aaactcgtca
    21061 agattggacg acacgatgca caggaagcgc aggcgttcca aaagggggac gtttttgtct
    21121 tccgcctgtg ccaacacgcg gcggttgaat gccagcaggc ttagttcgcg gcagaggatg
    21181 cggttttgtt cgggcataag gttctcccaa agatggttgt tgttcggtcg gagcgggcag
    21241 gcaatgccgt ctgaaggctt ggcggcatct gcgccggttt cagacggcat gacgtggcgg
    21301 acagtctgtt tcaggcatcg gggcaatgtg aaaacagata ccggcgcgtg cggtatccgt
    21361 ctgtttcaaa tcacttccaa cacaaaataa tgacgcagtt tttcgtacac cgcatcgctg
    21421 acgttgatgc agccgttggt cataatcctg tcggacacgg acggggaggc gatgcgttcg
    21481 ttccgccttt ccgacggtat ctgattccaa acgcggtgca gggcgaaaag aaaatcaccc
    21541 tcctgcttga aaccgatgac ttcgccgccg tatccgggct tgtcggtact gttcagcgtc
    21601 agcccgaacg tccccttggg cgtggcagtg ccgatgagga cggggtggca ctgacggtcg
    21661 tcagcgaaac agagttccgc tttggatgta tcgacgatga cttttttctt ttggatatag
    21721 gcattgaccg catccggctg cccctgtgca tatacggggg caaccatcga taggtcgcac
    21781 aatatcccta aaagcaggcg caacggcata gcggcttatt ggcggatgcg tttgggtttg
    21841 gcgggcacga tttcgcggat gatgacttgc ggctcggctt tgggtgctgg cggcggacag
    21901 gcggcatctt tcgggaagac ggggttccag tagaagctgc gggcaaattt gtctttatcg
    21961 aaaatcactt tgtattggca ggtagtaaca tcttccacgc cggaagtgtt ttcagggtcg
    22021 gtacctacgc ccggggtatg gaagtggaac aggtaatccc attcgcgcac gccgtacata
    22081 ccttcgtcgt aatgcgggcg gcccaggatt ttgtagatgt cgtctttggt caggccgggg
    22141 cgcatcaagt ccaattcgtc ataggtcggg aatgtgccgc gcttgttgtc gagcgttacg
    22201 gaatagggtt tcgggaaaac cggattgtcg gtcgtgccgt cggctttgac gttgcttttg
    22261 gttgcgcagg cagacaggat gcccgccgcc aatactgcca agcccggttt gacgatttgt
    22321 ttgattttca tatacagaat cctcttttac ccgatgcccg tctgcctttt gttcggaaga
    22381 gccgcttcag gatggtttta aatatttgat tttgattttt aatggtagca taaaacaacg
    22441 ggcgtatcag caggttcggc gtatttgtgc ggcatacggg gcgaagggtt tgccgtgtcg
    22501 cccgcctcct gccggcaatc tgccgattcg ccgcttccgg caacctgtta taattgatgc
    22561 cgactttatt tttccaacgt ttttaagcga aaacagaatg actatgcaag aacaataccg
    22621 gcccgccgcc atcgagcctg cggcgcagaa aaaatgggat gatgcccgta ttttcaacgt
    22681 ctccgaagac gcttccaaac ccaaatacta ctgcctctct atgttcccct atcccagcgg
    22741 caagctgcat atggggcatg tgcgcaacta caccattggc gacgtattga gccgcttcaa
    22801 acgcttaaac ggcttcaacg tcatgcagcc tatgggttgg gacgcgttcg gcatgccggc
    22861 ggaaaacgcg gcgatgaaaa acaacgtcgc ccccgccgca tggacttacg acaacatcga
    22921 atacatgaaa acccagctca aaagcctggg ttttgcgatt gactgggcgc gcgaaaccgc
    22981 cacctgcaaa cccgaatact accgctggga acaatggctg tttaccaagc tgtttgaaaa
    23041 aggcatcgtt taccgcaaaa acggcacggt aaactgggac cctgtcgatc aaaccgtcct
    23101 tgccaacgag caagttatcg acgggcgcgg ctggcgttcg ggcgcgttga ttgaaaaacg
    23161 cgaaatcccg atgtattact tcaaaatcac cgattacgcc gaagagttgc tcaacgattt
    23221 ggacaagctg gaacactggc cggaacaagt caaaaccatg cagcgcaact ggatcggcaa
    23281 atctcgcggt atgaccgtgc gctttgccgt ttcagacgac agcaaacaag gtttggaagg
    23341 cgattacgcg agattcctgc aagtttatac cacccgcccc gacacgctga tgggcgcgac
    23401 ttatgttgcc gttgccgccg agcatccgct ggcaaccgca gccgccgccg acaaacccga
    23461 attgcaggca tttatcgccg aatgcaaagc gggctccgtt gccgaagccg atatggcgac
    23521 gatggagaaa aaaggcgtgc cgaccggtcg ctacgtcgtc aacccgctca acggcgataa
    23581 gctggaagtg tggattgcca actatgtatt gtggggctac ggcgacggcg cggtgatggc
    23641 ggttccggcg cacgacgaac gcgatttcga gtttgccacc aaatacagcc tgccgaaaaa
    23701 acaggtcatt gccgtcggcg acaacgcatt tgacgcaaac caatggcaag aatggtacgg
    23761 cgacaaagaa aacggcgtat tggtcaacag cggcgacttg gacggcatga attttcagac
    23821 ggccttcgac gccatcgccg ccaagctgca aagccaaggc gcgggcgaac cgaaaaccca
    23881 ataccgcctg cgcgactggg gcatttcgcg ccaacgctac tggggctgcc cgattcccat
    23941 cgtccattgc gaacaatgcg gcgacgtacc cgtccctgcc gaccaactgc ccgtcgtcct
    24001 gcctgaaaac gtcgtacccg acggtatggg ttcgccgctg gcaaaaatgc ccgagtttta
    24061 cgaaactgcc tgcccgtgct gcggcggcgc ggcgaaacgc gaaaccgaca ccatggacac
    24121 cttcatggaa tcaagctggt atttcttccg ctacatgtcg cccaagtttt cagacggcat
    24181 ggtatcggcg gaagccgcaa aatactgggg cgcggtcgac caatacatcg gcggtatcga
    24241 acacgcgatt ctgcacctct tgtacgcgcg tttcttcacc aaactgatgc gcgacgaagg
    24301 tttggtcaat gttgacgaac cgtttgaacg cctgctgacg caaggcatgg tcgtttgcga
    24361 aacctactac cgtgaaaacg acaaaggcgg caaagactgg atcaaccctg ccgatgtcga
    24421 gctgactttc gacgataaag gccgccctgt ttccgccgtt ctgaaagcag acggactgcc
    24481 agtcgtcatc agcggcacgg aaaaaatgtc caagtccaaa aacaacggcg tcgatccgca
    24541 agagctgatt aacgcctacg gcgcggacac cgcccgcctg ttcatgatgt tcgccgcacc
    24601 gcccgaacag tccctcgaat ggagcgacag cggcgtcgaa ggcgcacacc gcttcctgcg
    24661 ccgcctgtgg cgtaccgttt acgaatacct gaaacaaggc ggcgcggtca aagcgtttgc
    24721 aggcaaccaa gacggtttgt ctaaagaact caaagatctg cgccacaaac tgcattccac
    24781 catcgccaaa gtcagcgacg actacggccg ccgccagcag ttcaacaccg ccatcgccgc
    24841 cgtgatggaa ttgctcaacc aatacgacaa aaccgacacc ggcagcgaac aaggccgcgc
    24901 cgtcgcccaa gaagtattgg aaaccgccgt acgcctgctg tggcccatcg tgccgcacat
    24961 ctgcgaaacc ctgtggagcg aattgaacgg cgcgaaactg tgggaagcag gctggccggc
    25021 agtcgacgaa gccgccttgg tcaaatccga aatcgaagtc atggttcaag tcaacggcaa
    25081 actgcgcggc aaaatcaccg tcgccgccga cgcatccaag gccgacctcg aagccgccgc
    25141 ccttgccacc gaaggcgcgg tgaaattcat ggaaggcaag cccgcgaaga aaatcatcgt
    25201 cgttcccgga cgcttggtaa atatcgtcgt ctaagccgct tttaaggttt agccatacgg
    25261 ataaaggccg tctgaaactt ggaaacaggg tttcagacgg cctttttaag gcagattggg
    25321 ttgtccgcat gacagacctc aaatatagtg gattaacttt aaaccggtac ggcgttgccc
    25381 cgccttgccc tgctgtctgc ggcttcgtct ctttgtcctg attttgttaa tccactaaaa
    25441 cttaatctca aaatccaaaa catgaattta tttttcgata ctgaattggg gaagcaacaa
    25501 aataaagcaa cccacaaaat ccgtgtaatg agcgaggctt ggttggaaaa aaacggctac
    25561 tgcccctgtt gcggaagcaa gccgatgaag agatttgcca ataacaaacc tgttgcagac
    25621 ctcttttgcc caaattgcca cgagcaatat gaattaaaga gtaaaaatca aaaaaccata
    25681 ggcaacatcg tgcctgacgg tgcatatcac accatgttgg agcgcatccg gtcagatacc
    25741 aaccccaact ttttctttct tgcatataaa aaagcggatt actccatacg gcaattggtg
    25801 cttgtaccca aacatttcat cacgccggat atgattattc ccagaaataa aggcattaaa
    25861 aaccggccgc accacattat gtgttccatc aatctcgccc ctttgcctga aagcggaaaa
    25921 atattcttaa tagacgattc ccgcattatc gaacccgaaa ccgttctgaa aaaatggcaa
    25981 tctaatctgt ttttacgcaa ccaaaatgcg gagcgcaaag gttggctttt ggctgttatg
    26041 aaatgtatcg accaactccc cgaagaattc acattgtcgc aaatgtatga atttgaaaac
    26101 aaactatcca tccaatttcc ccaaaacaac catatcaaag acaaaatccg ccaacagttg
    26161 caaattttgc gcgaccaaaa tacgatcgaa ttcattggtc gcggacttta caaaaaaatc
    26221 gacaaattgc gtccgacttc caaggcggtt tgatttcaaa tcatgatact gaattcacaa
    26281 gggatattta ttcctttgct attctgaaaa cagttgaagt tcagcgcaat attgccgaaa
    26341 aaggcagcag ccgtaaaaaa gtcggggaat tattggcaat ttatgcgtaa tttcgacaaa
    26401 ttggctgaag aagctgccga acacgccgct gaaggcgcgg tgaaattcat agaaggcaag
    26461 cctgcgaaaa aaatcatcgc cgtaccgggc agactggcga acatcgtggt gtaaatcttt
    26521 ttaaaaacat taaacaatgt taagatgccg tccgaaatcc gttttggatt cagacggtat
    26581 ttttttgaaa tttaattttt taaggagtaa acctaaatat gaaatttcct tccttccttc
    26641 cttccttcct tcctttaaac ggatactctg cctgtcggca gtaatctcgg tattgggggc
    26701 ttgtacggtc gttgcagatg tttacggtca ggattccgcc acaatgaatg ctgcggctgc
    26761 cgaagattat atgaaaacgg ttgagttgaa caagtctgcc ggcaatgtcg atactacatc
    26821 caaaacagcc cgtagggtgc aggcagtatt tcgacgtatg ttgccttatg ccgatgcggc
    26881 aaataatacc ggccataagt ttgactggaa aatgacggtt ttcaaaaacg atgagctgaa
    26941 cgcgtgggca atgcccggtg gaaaaatggc gttttatacg gggatagtcg acaaactcaa
    27001 gctgaccgat gacgaaattg ccgccattat ggggcatgaa atgacgcacg ccctgcatga
    27061 acacggtaaa aataaggtcg ggcaacaaat tttgaccaat acggcggcgc agataggcac
    27121 gcagattata ttagacaaaa aaccggacac taatccggaa ttggtcggat tgggtatgga
    27181 tattttggga acgtacggta ttaccttgcc ttatagccgc agcttggaag aagaagccga
    27241 tgagggggga atgatgttga tggcgcaggc aggctatcat ccggcggccg ctgtcagggt
    27301 ttgggaaaaa atgaatcagg aaaacgacca aaacggcttt atttatgcta ttacctctac
    27361 tcatccgaca aacaatgccc gtatagaaaa tctaaaacgg ttgttgccga ccgttatgcc
    27421 ggtttatgag caaagtgtca gaaataaggg gcgcgttaat ccactataaa aaacgtcggc
    27481 gttaagggaa ggggatcgag aatttgagcc gttgtttcaa aatgccgtct gaaatcgttt
    27541 gagattcaga cggcatttaa acagttctgc gcccgcccgt tttgccttca agcccgcgcc
    27601 gcttaaatcc aatcccccct gcaacgggcg caaaaaaatc tccgtgcctc gcggtcgggg
    27661 atgtttcggg tttcacgccc gtctgcgccg tgccttgctg tcgggctatg ccgtctgcac
    27721 cgccgccgtt tcggcttggt ttgatgcggt cgggcatttg tgcggctacg ccctgatatt
    27781 cggttcggcg gtcttgcctg tttcgtcagg catattcagc agcgcgtcta ttgtgaactt
    27841 gctgattttg ccgtttgcca agtcgtctat gcaatggctt cgatgccctt gtttttgacg
    27901 atggtcagca gcttcaacgc cgcgccgctg ggctttttaa cgccccgctc ccaagccgaa
    27961 acgtgatttt ttcccacgtt gagatagatg gcgaaagcgg cttgcgatag tgcctccttc
    28021 tccctgattg ccttgatgtc tccgccgctc aacggtttga tttcggtcag gcaggacttg
    28081 tcaaagccgc gcatagtctt tttgtcgatt gcgccgatgt tgtaagcccc ttccatcatt
    28141 tcatgaatgg cggctaatgc ctcgcttttg tatttcatga ttctatctcc gtgataatgc
    28201 cgtcttcttt caaagccgcc agctctgccc gcgtgtattt cagataatat gcggcggctt
    28261 ttcggtaaac gtccagttct ttatccgaaa tgttttctct gtcgttcttg gcaaaggcgt
    28321 aaacgaaaaa tgccctatct gcctgtttga acaatatcag gctgcgatag ccgccgcttc
    28381 tgccttgtcc ttgccttgct atgcgctgct taatcacacc gccgcctaaa tctgcgtcta
    28441 tcagcccgtt atccgccctc tctaccgctt ccagcagctc ggaatcactg attttatgtt
    28501 tcttggcaaa tttcactatc cattggtttt tgaatatccg cattgtgtag ccgttgatta
    28561 gtgatacttg gaattatacc agagacagac aacacggcaa gctgcctttt ttatgcccca
    28621 caatttctcc gtttatgccg cctgaaacac tggcggctaa cctgattatt ttttaatcaa
    28681 tcgcttttaa tggctcaaat tgcgttttta gcgcgtttcg ctgtcttcct attccccccg
    28741 tatataacca agcggtttaa ccgccattcc tgctcaaaat aggcgcggtt ccgtgcgttt
    28801 tggcttttca ttccgctacg ctcccactgc caagacggtg aaaaaccgga aaaccccaaa
    28861 agttgttacc gattcgttac catttgttac cgcatttgtt accgcatttg ttaccaattt
    28921 tttatctttt tcttatttta tagtgaatta aatttaaacc ggtacggcaa ggcgaggcaa
    28981 cgccgtaccg gtttaaattt aattcactat agtataggtt ttttccaaaa acccaaaaaa
    29041 aaccgctctg catcttggac ggtttccccc tcattcccta cggtgcgtca gtattccccg
    29101 caagctattt cccgcttggc attcctgatt tggtggtcgt tcagctttat ttttacggta
    29161 ttttctcgcg gtattttcgg atgctgtaaa aaatcaggtt agttcaatta gggcggattg
    29221 gacgggattg cactgtcagg aagggggacg gcaggataac aatcagcctg aaatccttgt
    29281 attgattgga attggttgac agcgttggat gggattgaac aaaaaacgcc tgaaattttc
    29341 aggcgttttt gtgtgttggt gccgacagcg agatttgaac tcgcacagcc tacggccact
    29401 accccctcaa gatagcgtgt ctaccaattt caccatgtcg gcatttgaaa aactgttatt
    29461 tctgctgctg aggaacaggg gcagaaggtt cggtattgct tacgggtttg ggtgcttgct
    29521 gagtttgttg tacgttgctg aagtccaaac cgtgttttgt cgtgtgggtg tgaatataca
    29581 ccatagccat gcaggttgca aagaaaaatg ttgctgcaac ggcggtcgag cggctgagga
    29641 agttagcgtt gccggcagag ccgaatacgc cttgcgcgct gccgcttccc gatccgaaag
    29701 tcgcgccggc atccgcgcct ttgccgtgtt ggagcaatac taacacgatg acggccaaag
    29761 cggaaattat attaacaatc caaattaggg ttttgaaggc ttccatattt ttctacgcat
    29821 tttgtgcggc actgatgatg gcggtaaagg agtcgtacga caatgacgcg ccgccgacga
    29881 gtgcgccgtc cacataaggt actgcgaaga tgtcggccgc gttgtccgct ttcacacttc
    29941 cgccgtaaag gacacggatt ttaacatcgc ttccgcacaa agacaagatt tctttgtaga
    30001 tgaatgcgtg catatcggca atctgttcga cggtggcgac tttgccggtg ccgatcgccc
    30061 agacgggttc gtaggcgacg gcgatgtttt tggtatccag cccttgcagg atggaaagct
    30121 gatgggcgat gacttcgtgt tctttgccgg cttcgcgctc ttcgaggctt tcgccgacgc
    30181 acaataacgg gatgagtccg acgttgagga cgttttccat tttgcggcgt tggatttcgt
    30241 ttttttcgcc gaaataaagg ctgcgttcgg agtgtccgat gaggacgatg tctgtgccgg
    30301 tgtcggcgag catttcggcg gacacttcgc cggtgtacgc gccgttgtcg gggaagcggc
    30361 tcacgtcttg ggcgcaggtg aggatgcggt tgtttaagac gatttgcatg gcgttgtgca
    30421 gttgcagcag gtaaacggtc ggggcggcga gtccgatgag gacgcgttcg gcggtgggga
    30481 ggatgcggaa gcggtgcatg agcgaattgt tgttttggag ccggccgttc attttccagt
    30541 tgccgatgac ccatttttga tcccacattc cgatttggtg atacatcttt tttgctccgt
    30601 gtcgtgtttt ttctgtctgc cgcgtgtggc gcgttgcaat gtgaagttta gtggatatgc
    30661 ggcgggttcg caacttgggg cgggcggctg cgggggcggt ttggaatgtt gtttcgggca
    30721 ggttgtttta taatggccgc ctgatatgta tgcaactata ggagatgtga tgcacgcgct
    30781 tcatttttcg gcttcggaca aggccgcgct ttatcgggag gtgttgccgc agattgagtc
    30841 tgtggtggcg gacgagacgg attgggtggc gaatttggcg aacacggcgg cggttttgaa
    30901 ggaggcgttc ggttggtttt gggtgggttt ttatttggtc gatacgcgtt cggacgaatt
    30961 ggttttggca ccgtttcagg ggcctttggc gtgtacgcgg attccgttcg gtcgcggggt
    31021 gtgcggtcag gcttgggcga agggcgggac ggtggtcgtc ggggatgtgg acgcgcatcc
    31081 cgaccatatt gcctgttcgt ctttgtcgcg ttcggagatt gtcgtgccgc tgttttcaga
    31141 cggccgctgt atcggcgtgt tggacgcgga cagcgaacat ttggcgcagt ttgatgagac
    31201 agatgctttg tatttgggcg aactggcgaa gattttggag aagcggtttg aggcttcgcg
    31261 ccaggcggct tgagtgaggg aaaggggcgg gcggcgcgtg ccgaagttgg cgcggcggga
    31321 gtgtggtttt ataatgcctg ccattgttga aacaattatt tgacggagca ctaaatggat
    31381 tttgaaaaag cgcggttcaa tatggtcgaa cagcaaatcc gtccttggga tgtattggat
    31441 tttgacgttt tggacgcttt ggcggagatt ccgcgcgagc tttttgtcga tgaggacttg
    31501 cagggtttgg cgtatgcgga tatggcgctg ccgcttgcca acggtcataa gatgctcgag
    31561 ccgaaagtcg tggcgcggct ggcgcagggc ttgaagctga cgaaaaacga tacggttttg
    31621 gaaatcggca cgggttcggg ctatgcgacc gcgctgttgg caaaattggc tggtcgtgtg
    31681 gtgtcggacg acatcgatgc cgaacagcaa aaccgcgcca aagcggtgtt ggacggcttg
    31741 ggtttggaca atatcgatta tgtgcaaaac aacgggttga ccgaactttc cgcaggtgcg
    31801 ccgtttgatg cggtttatgt cggcggtgcg gtaaaccttg tgcctgaagt gttgaaagaa
    31861 cagttgaaag atggcggacg catggtggtt atcgtaggcc gcaagcctgt tcagcgcgcg
    31921 cttttgatta cgcgcaaggg cgatgtgttt gaagagaagg tgctgttcga taccttggtg
    31981 gcgcatttgg atgataagga tgccgatccg tttggcggtt ttcagttttg atgttcggat
    32041 tgcgatgccg tctgaaagcg ggtttggggc ttcagacggc attttgcttg gtttttttgg
    32101 gggtgcgatg gatattgtgc aacttgggcc cgcggaattg aaagcgtgga tggacgaagg
    32161 gcggatgttt tgtttgttgg acgtgcgtac ggatgaagaa acggcggttt gttcgctgcc
    32221 aaatgcgctg catatcccga tgaatctgat tccgctgcgg caaaacgagc tgccggatga
    32281 tgtgccgctt gtggtgtatt gccatcacgg tatccgcagc ctgcatacgg cgatgtatct
    32341 ggcggacgcg gggtttgaaa acctgtacaa ccttcagggc ggcatcgacg cgtgggcggt
    32401 tgagattgac ggcaacatga tgcgttattg aggcaggcat ttcagacggc attccttaaa
    32461 tgcgtgtacc tgtggtgttc caaaaggtcg tctgaaagtg cagcttctgc gaagctaaag
    32521 cgcggtatca acgaagttaa aatttgcctg aaccttaaag gcagcctgca ccccaatccc
    32581 ctctccccgt gggagagggc taggacgagg ctggcaaact gaaggtttgc ttgggcagca
    32641 ttttcaatgt gcaggctgct
//
LOCUS       DAWFHY010000016        31704 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.016, whole genome shotgun sequence.
ACCESSION   DAWFHY010000016 DAWFHY010000000
VERSION     DAWFHY010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 31704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31704
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.016"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            59..244
                     /locus_tag="WGQ68_000630"
     CDS             59..244
                     /locus_tag="WGQ68_000630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714480.1"
                     /translation="MDGFVVPAKAGIRNQKPQKFIGKTETQKQRKPNGQDSRLRGNDG
                     SYITQKIETKQMGFPLA"
     gene            complement(520..1266)
                     /locus_tag="WGQ68_000631"
     CDS             complement(520..1266)
                     /locus_tag="WGQ68_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEZ0714481.1"
                     /translation="MDFRFDIIYEYRWMFLYGALTTLGLTVVATAGGSVLGLLLALAR
                     LIHLEKAGAPMRVLAWALRKVSLLYVTLFRGTPLFVQIVIWAYVWFPFFVHPSDGILV
                     SGEAAIALRRGYGPLIAGSLALIANSGAYICEIFRAGIQSIDKGQMEAARSLGLTYPQ
                     AMRYVILPQALRRMLPPLASEFITLLKDSSLLSVIAVAELAYVQNTITGRYSVYEEPL
                     YTVALIYLLMTTFLGWIFLRLEKRYNPQHR"
     gene            complement(1445..2116)
                     /locus_tag="WGQ68_000632"
     CDS             complement(1445..2116)
                     /locus_tag="WGQ68_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEZ0714482.1"
                     /translation="MQIKKIMKWLPVALSLLGALGYTGYGSEAVRTAVAVLDVLGAAG
                     DAGSDAPARRRASAKSGHRYTGTVSKVYDGDTLHVIDGDGAKHKIRMAYIDAPEMKQA
                     YGTRSRDNLRAAAEGRKVSVRVFDTDRYQREVAQVSVGKTDLNLMQVQDGAAWHYKSY
                     AKEQQDKADFADYADAQIQAERERKGLWKAKNPQAPWAYRRAGRSGGGNKDWMDAVGE
                     WLGIW"
     gene            complement(2185..2856)
                     /gene="rpiA"
                     /locus_tag="WGQ68_000633"
     CDS             complement(2185..2856)
                     /gene="rpiA"
                     /locus_tag="WGQ68_000633"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEZ0714483.1"
                     /translation="MTTQDELKRIAAEKAVEFVPENEYIGIGTGSTINFFIEALGKSG
                     KKIKGAVSTSKKSSELLAQYDIPVVSLNEVSGLAVYIDGADEVNHALQMIKGGGGAHL
                     NEKIVASASEKFVCIADESKYVSRLGKFPLPVEVVESARSLVSRKLLAMGGQPELRIG
                     YTTFYGNQIVDVHGLNIDKPLMMEDEINKITGVLENGIFARDAADVLILGTEEGAKVI
                     YPCQG"
     gene            complement(2933..3415)
                     /locus_tag="WGQ68_000634"
     CDS             complement(2933..3415)
                     /locus_tag="WGQ68_000634"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEZ0714484.1"
                     /translation="MTNIRIGQGYDVHQLTEGRKLILGGVEIPFEKGLLGHSDADALL
                     HAVTDALLGAAGLGDIGSHFPDTAAEFKDADSRVLLRAAYQSVQAQGWQAVNVDTTVI
                     AQKPKLAPHIPQMRANIAADLGIDISCVNIKGKTNEKLGYLGRMEGIEAQAAVLLVRI
                     "
     gene            complement(3447..4136)
                     /locus_tag="WGQ68_000635"
     CDS             complement(3447..4136)
                     /locus_tag="WGQ68_000635"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEZ0714485.1"
                     /translation="MKRKNIALIPAAGIGARFGADKPKQYVEIGSKTVLEHTIGIFER
                     HEAVDLTVVVVSPEDTFADKVQTAFPQVRVWKNGGQTRAETVRNGVAKLLEIGLASAD
                     DNILVHDAARCCLPSEALTRLIEQAGNAAEGGILAISIADTLKRAEGGQISATVERTS
                     LWQAQTPQLFRAGLLHRALAAENLDGITDEASAVEKLGIRPLLVQGDARNLKLTQPQD
                     AYIVRLLLNAV"
     gene            complement(4133..4867)
                     /gene="dnaQ"
                     /locus_tag="WGQ68_000636"
     CDS             complement(4133..4867)
                     /gene="dnaQ"
                     /locus_tag="WGQ68_000636"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEZ0714486.1"
                     /translation="MTTRQIILDTETTGLYADGGDRLVEFAGLEMVNRQMTDKNLHLY
                     VHPERDMPEEAARVHGLTIEVLEGKNAPPFAEVGRQIADFLRGAELIIHNAKFDVGFL
                     NMEFRRMGLPTVEELGCTVTDTLAMAREMFPGQKASLDALCNRFSVDRSKRVLHGALI
                     DCELLGEVYLAMTRRQFDLMGEAAEEKMETKPVVHTETKRSGKLKVIRADENELAAHG
                     QYLDGLGEACIWRKEAVPSENGGTDA"
     gene            complement(5023..6333)
                     /locus_tag="WGQ68_000637"
     CDS             complement(5023..6333)
                     /locus_tag="WGQ68_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0714487.1"
                     /translation="MYAKKGGLGLVKSRRFAPLFATQFLGAFNDNVFKTALFVMIGFY
                     GLGQNGFLPAGQMLNLGALLFILPYFLFSSLSGQLGNKFDKAVLARWVKVLEMIIMAV
                     AAYGFYIRSAPLLLACLFCMGAQSTLFGPLKYAILPDYLDDKELMMGNSLIESGTFVA
                     ILFGQILGTAVAGVPPYIVGILVLLVAVGGTVGSLFMPSVPAKAADTQIEWNIVRGTK
                     SLLRETVRHKPVFTAIIGISWFWFVGAVYTTQLPTFTQIHLGGNDNVFNLMLALFSIG
                     IAAGSVLCAKFSRERLMLAWVTVGALGLTVCGLVLVWLTHGHRFEGLNGIFWFLSQGW
                     AYPVMAVMMLIGFFGGFFSVPLYTWLQTASSETFRARAVAANNIVNGIFMVSAAVLSA
                     VLLFVFDSISLLYLIVALGNIPLSVFLIKRERRFLGAAAIRKKP"
     gene            complement(6323..6508)
                     /gene="ccoS"
                     /locus_tag="WGQ68_000638"
     CDS             complement(6323..6508)
                     /gene="ccoS"
                     /locus_tag="WGQ68_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HEZ0714488.1"
                     /translation="MESMFILVPISIILAFVIGWFFWWSGKNGQFDDLEGPAHRILMD
                     DDSTSKLIEKERENDVR"
     gene            complement(6508..6807)
                     /locus_tag="WGQ68_000639"
     CDS             complement(6508..6807)
                     /locus_tag="WGQ68_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714489.1"
                     /translation="MHYFSIHDQSGRHIGFFILLADDESEARPQSGRFAVKLEGGMEN
                     HVLSPFGQTEIPQYWRVIKDRIELFFDEAPVGTLRNEYLTVSGQTFVLNDLTGNM"
     gene            7158..8354
                     /locus_tag="WGQ68_000640"
     CDS             7158..8354
                     /locus_tag="WGQ68_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0714490.1"
                     /translation="MSQKLILVLNCGSSSLKGAVLDNGSGEVLLSCLAEKLNLPDAYI
                     TFKVNGEKHKVDLSAHPDHTGAVEALMEELKAHGLDSRIGAIGHRVVSGGELYSESIL
                     VDDEVIAGIEKCIPLAPLHNPAHLLGLRAAQSIFKDLPNVVVFDTSFHQTMPEVAYKY
                     AVPQELYEKYGLRRYGAHGTSYRFVADETARFLGKDKKDLRMVIAHLGNGASITAVAN
                     GESRDTSMGLTPLEGLVMGTRSGDIDPSVFGFLAENANMTIAQITEMLNKKSGLLGIS
                     GLSNDCRTIEEEAAKGHKGAKLALDMFIYRLAKYIGSMAVAAGGLDALVFTGGIGENS
                     DIIRERVIGYLGFLGLNIDQEANLKARFGNAGVITTADSKAVAVVIPTNEELMIAHDT
                     ARLSGL"
     gene            complement(8764..10605)
                     /locus_tag="WGQ68_000641"
     CDS             complement(8764..10605)
                     /locus_tag="WGQ68_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HEZ0714491.1"
                     /translation="MQTNYLSETDSIMKKLICLFAVFLMLCGRAFALDANDLLPPEKA
                     FVPELAVADDGVNVRFRIADGYYMYQAKIVGKTDPADLLGQPSFSKGEEKEDEFFGRQ
                     TVYHHEAQVAFPYAKAVGEPYKLVLTYQGCAEAGVCYPPVDTEFDISGNGTYHPQTDE
                     PASAKDRFLQPSSQNGSGVLPPPKGDEGGDSRFKLSWDTLNANLLAFFLAGLGLSFTA
                     CMYPLLPIVSSIVVGDKKAGKARAFVLSVVYVQGLALTYTLVGIVAGLTGALLTVWLQ
                     QAWVVLAASALMVVLALSMFGLFNIQLPNAVQSYFQNQSSRLSGGKIVSVFIMGILSA
                     LIVGPCVAPPLAFALGYIGQTGDAVLGGLALYTLALGTGVPLIAIGTFGGHILPKAGD
                     WMNAVKYAFGFILLAVAVYLATPHLPYYLVVALYTLLMLVPACMLLVNGRRQKRRPKA
                     VAFVLGGILLIGGAWFGWQGANGKTTALHHFLTLNPPAEAGKSSEHGKMFADTAALKA
                     AMDTALKEHPDKPVVLDFYADWCISCKEMAAYTLNQPEVHQAVDMERFFQIDVTANMP
                     EHQALLKEYGLFGPPGVFVVRSDGSRSEPLLGFVKADKFIEWYEQNR"
     gene            10696..11166
                     /locus_tag="WGQ68_000642"
     CDS             10696..11166
                     /locus_tag="WGQ68_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1841 family protein"
                     /protein_id="HEZ0714492.1"
                     /translation="MYDVNTHDVRRFFARVWQQRLNPLQLSALEQKALRIVEAHPEYH
                     CYLERIEDHLDTDWLPENGESNPFLHMSLHLSVQEQAGIDQPHGIRAIHDTLCAKHGW
                     LEAEHEMMEALAETLWTAQRYGTGLDVNFYMTRLRKLIGLGAENQARLNPHEIA"
     gene            complement(11239..12051)
                     /gene="hpnC"
                     /locus_tag="WGQ68_000643"
     CDS             complement(11239..12051)
                     /gene="hpnC"
                     /locus_tag="WGQ68_000643"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HEZ0714493.1"
                     /translation="MSVGHYENFPVGSLILPRRLRKPVHAVYAFARTADDMADEGSMP
                     SEARLAGLEGLQRELDVLASGGRSAHPLIARLDAEAVVPFGLDLQPFYDLLSAFSQDV
                     VKTRYGNFGELADYCRRSANPVGRIMLALYGKTDAVCVAQSDGICTALQLVNFWQDVA
                     VDWQKGRVYIPQDDLLKFGVSEGQIAEGRADAAFQRLMAYECRRAFRMLKAGSPLARE
                     LKGRIGLELRMIVLGAQLILQKLDACRYDVFAQRPVLDKKDWLIMLKKALWK"
     gene            complement(12138..12620)
                     /locus_tag="WGQ68_000644"
     CDS             complement(12138..12620)
                     /locus_tag="WGQ68_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0714494.1"
                     /translation="MAIVKNSVVSLHYEMYDANNQLLDKTEEPIAYLHGGYDGIFPLV
                     EEALHGKDAGDTVDVALSPDDAFGEQDPELVRIEDAGVFPVEVEVGMMFEADDPETGD
                     VVVYRVTDVADGKAVVDGNHPLAGMKIRFKATVESVRDASDEEIAHGHVHGPHGHHHH
                     "
     gene            13093..13374
                     /locus_tag="WGQ68_000645"
     CDS             13093..13374
                     /locus_tag="WGQ68_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714495.1"
                     /translation="MKKSLFAAALLSLVLAACGGEKAAEAPAAEAPAAEAPATEAPAA
                     EAPATEAPAAEAPAAEAPAAEAPATEAPAAEAAATEAPAAEAPAAEAAK"
     gene            13484..14851
                     /locus_tag="WGQ68_000646"
     CDS             13484..14851
                     /locus_tag="WGQ68_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0714496.1"
                     /translation="MPDLHTEFSRLLPADEIAELSPTLLKDQRNRFTSAPDIILQPRS
                     VESVQTIMRFCYEHRIPVTPQGGNTGLCGAAVSENGVLLNLSKLNRIRSINLSDNCIT
                     VEAGSVLQTVQQAAEASNRLFPLSLASEGSCQIGGNIACNAGGLNVLRYGTMRDLVIG
                     LEVVLPNGELVSHLHPLHKNTTGYDLRHLFIGSEGTLGIITAATLKLFARPSDKATAW
                     VGIPDIESAVRLLTETQAHFAERLCSFELIGRFAAELSSEFSKLPLPTHSEWHILLEL
                     TDSLPDSNLDDRLVEFLYKKGFTDSVLAQSEQERIHMWALRENISASQRKLGTSIKHD
                     IAIPIGRVADFVRRCAKDLEQNFKGIQIVCFGHLGDGSLHYNTFLPEILSNEVYRYEN
                     DINDAVYRNVLACNGTIAAEHGIGIIKKQWLDKVRTPAEIALMKSIKQHLDPYNIMNP
                     GKLLP"
     gene            14886..15392
                     /locus_tag="WGQ68_000647"
     CDS             14886..15392
                     /locus_tag="WGQ68_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HEZ0714497.1"
                     /translation="MGMTATYQLVDNEKLALLKNTDTEWQELFEELEDLEDEAAVCLD
                     IDKMWDVLHFVLTGTSAENPLKGNLLSESIVGTAPISDDDGYAAYIESAQIHEIISAL
                     EGFDIKSAMEHFSMQVCKEHHIYPDIWDYEDEIYDIKEEITDYFYKIGNFYKTARHSN
                     SNVLVSIY"
     gene            15483..15713
                     /locus_tag="WGQ68_000648"
     CDS             15483..15713
                     /locus_tag="WGQ68_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714498.1"
                     /translation="MSKSYRKHPFSPITTAVSEKQDKRLVNRKLRRIARECLKQGKEP
                     PHHIRAVSNVYDFAKDGHFRFSASRHPHLLRK"
     gene            complement(15820..16266)
                     /gene="smpB"
                     /locus_tag="WGQ68_000649"
     CDS             complement(15820..16266)
                     /gene="smpB"
                     /locus_tag="WGQ68_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEZ0714499.1"
                     /translation="MAIANNKKAFHDFFIEDRIEAGLVLEGWEVKAIRAARVQLKESY
                     IYWKKDAFYLVGCHITALPTASTHIKPDAVRPRKLLLNQSEINKLIGKTERAGYTIVP
                     LDLHFSRGKIKMEIGLAKGKKQHDKRQSMKEADWKREKQRLIKHTR"
     gene            complement(16321..17331)
                     /gene="waaF"
                     /locus_tag="WGQ68_000650"
     CDS             complement(16321..17331)
                     /gene="waaF"
                     /locus_tag="WGQ68_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEZ0714500.1"
                     /translation="MSIKILIISPSWIGDCVMTQPLFRRLKELHPGCTIDVFAPKWSM
                     AVFERMPEVNEILENPFGHGALELKRRWRVGRDLGRRGYDQVIVLPGSLKSAIIALAT
                     GIGKRTGYVGESRYFLLNDIRRLDKERLPLMVDRYTALAHPSQEDFDGHSGFPEFSID
                     ERRREISVETFGLNLGKPVLAFCPGAEFGPAKRWPAKHFAELGKHYSATGWQVWLFGS
                     QKDDEIAEEINCLSDGMCVNLCGKTDLSQAMDLLSLADTVVCNDSGLMHLAAALGRKV
                     VAVYGSSSPTHTPPLSDRAKIVSLHLECSPCFKRECPLGHTDCLNRLYPEKIVQAVEE
                     AV"
     gene            complement(17462..17537)
                     /locus_tag="WGQ68_000651"
     tRNA            complement(17462..17537)
                     /locus_tag="WGQ68_000651"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17502..17504),aa:Asn,seq:gtt)
     gene            complement(17728..18480)
                     /locus_tag="WGQ68_000652"
     CDS             complement(17728..18480)
                     /locus_tag="WGQ68_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEZ0714501.1"
                     /translation="MITLPSLNNLPSKWDEIRYWLETRVCECGVMPHPNLTECEAGQF
                     DRDFWDNIGCAPEEYVRIRRAIRLLEARYPDSLNELVCAAIATPLGEMLAVFGNKGLC
                     LLEFVGQKHLEQEIAAVQKALRGRFVFREDERTQLLRQELDLYFKGRLKTFATPLEQI
                     GTEFQKQAWDALLAIPYGETRSYKEQAQRLGNPKAVRAVAAANGQNKVSVLIPCHRVI
                     GSDGKLTGYAGGLNRKQFLLALERGEVQTALF"
     gene            18791..19315
                     /locus_tag="WGQ68_000653"
     CDS             18791..19315
                     /locus_tag="WGQ68_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HEZ0714502.1"
                     /translation="MQTRILSAVLLAFSTAAFAGGAFTLQFDNPSEDGGFTQNQLLSA
                     PYGFGCSGGNASPALSWKNPPAGTKSFVLTVYDKDAPTGLGWMHRVVADIPADVHRRN
                     ATSLQLSRCANIADDQSAAISAVISLRICRIRLTPSYTAKPMPSCCNHANTPQSATSA
                     ALCGTSSSVSTAAA"
     gene            complement(19404..19683)
                     /locus_tag="WGQ68_000654"
                     /pseudo
     CDS             complement(19404..19683)
                     /locus_tag="WGQ68_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(20055..21200)
                     /gene="dapE"
                     /locus_tag="WGQ68_000655"
     CDS             complement(20055..21200)
                     /gene="dapE"
                     /locus_tag="WGQ68_000655"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEZ0714503.1"
                     /translation="MTETQSLELAKALISRPSVTPDDRDCQKLLVERLYKIGFAAEEL
                     HFGDTKNIWLRRGTKVPVVCFAGHTDVVPTGPVEKWDSPPFEPTERDGRLYGRGAADM
                     KTSIACFVTACERFVAEHPDHQGSIALLITSDEEGDALDGTTKVVDVLKARGELIDYC
                     IVGEPTAVDKLGDMIKNGRRGSLSGNLTVKGKQGHIAYPHLAINPVHTFAPALLELTQ
                     EVWDEGNKYFPPTSFQISNINGGTGATNVIPGELNVKFNFRFSTESTEAGLKQRVHAI
                     LDKHGVQYDLQWSCSGQPFLTQAGKLTDVARAAIAETCGIEAELSTTGGTSDGRFIKA
                     IAKELIELGPSNATIHQINENVRLNDIPKLSAVYEGILARLLAGNAV"
     gene            complement(21314..21934)
                     /locus_tag="WGQ68_000656"
     CDS             complement(21314..21934)
                     /locus_tag="WGQ68_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenosine tetraphosphatase"
                     /protein_id="HEZ0714504.1"
                     /translation="MLTPKSCDLFNIPFFQFSQLKKYQPESIPQIKADYKENWQIWQQ
                     LIQQVAAELGAPFAPPHIERWCNGWQVRAHFFAYFKYEQYKNSAAILSILLNRCRLSV
                     SLDWHCYKADVSPIALPDYNRWLDNFDTEKYASFDMWHGAESEYDDYRTVAQQNESDR
                     KLQNDEDFFCIGKHIERDDLGRQDVAKWIAETVEDLLPLYEACHGK"
     gene            complement(21968..22477)
                     /locus_tag="WGQ68_000657"
     CDS             complement(21968..22477)
                     /locus_tag="WGQ68_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0714505.1"
                     /translation="MNLFETQSVTFAEPIEMLYACHGKVRRFCGQVAMLSDYIAENGC
                     NQLVLQTIRQIAQYFNVAAPLHHEDEEENFFPLLLQYAPQAQESVDELLRQHIGLHDN
                     WAAVSAEFAKLEADNAYVPDEEAFKRFVAGYDVHLAIEEPLFDMGNTFIPKEKLTEIG
                     EIMAARRRK"
     gene            complement(22573..23124)
                     /gene="azu"
                     /locus_tag="WGQ68_000658"
     CDS             complement(22573..23124)
                     /gene="azu"
                     /locus_tag="WGQ68_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="azurin"
                     /protein_id="HEZ0714506.1"
                     /translation="MKAYLALISAAVIGLAACSQEPAAPAAEATPAAEAPASEAPAAE
                     AAPADAAEAPAAGNCAATVESNDNMQFNTKDIQVSKACKEFTITLKHTGTQPKTSMGH
                     NLVIAKAEDMDGVFKDGVGAADTDYVKPDDARVVAHTKLIGGGEEASLTLDPAKLADG
                     EYKFACTFPGHGALMNGKVTLVD"
     gene            complement(23405..23827)
                     /locus_tag="WGQ68_000659"
     CDS             complement(23405..23827)
                     /locus_tag="WGQ68_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEZ0714507.1"
                     /translation="MNLEQLGRRDALLSGLLKQAGQWRRLDAAVKKLLPANLHPHFQT
                     ACIEDGRLVLLAANNMAASRLKMIAPSVLPQLAGLDASIRSVSVRLVPKPEKPPKTNT
                     LHLSKAALESFGSAAVKLEKRHPELAEALANLVRRHGA"
     gene            23958..26708
                     /gene="secA"
                     /locus_tag="WGQ68_000660"
     CDS             23958..26708
                     /gene="secA"
                     /locus_tag="WGQ68_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEZ0714508.1"
                     /translation="MLTNIAKKIFGSRNDRLLKQYRKSVARINALEEQMQALSDADLQ
                     AKTAEFKQRLADGQTLDGILPEAFAVCREASRRTLGMRHFDVQLIGGMVLHDGKIAEM
                     RTGEGKTLVATLAVYLNALAGKGVHVVTVNDYLASRDAGIMEPLYNFLGLTVGVIISD
                     MQPFDRQNAYAADITYGTNNEFGFDYLRDNMVTDQYDKVQRELNFAVVDEVDSILIDE
                     ARTPLIISGQADDNIQLYQIMNTVPPHLVRQETEEGEGDYWVDEKAHQVILSEAGHEH
                     AEQILTQMGLLAENDSLYSAANIALMHHLMAALRAHSLFHKDQHYVIQDGEIVIVDEF
                     TGRLMSGRRWSEGLHQAVEAKEGVEIKRENQTLASITFQNYFRLYTKLSGMTGTADTE
                     AFEFQSIYNLETVIIPTNRPVQRKDFNDQIFRSAEEKFEAVVKDIEECHKRGQPVLVG
                     TTSIENSELVSHLLQKAGLPHNVLNAKEHEREALIVAQAGKVGAITVATNMAGRGTDI
                     VLGGNLKHQTDAIRADETLSDEEKQAQIAALEDGWQAEHDKVMEAGGLHIIGTERHES
                     RRIDNQLRGRSGRQGDPGSSRFYLSFEDPLLRLFALDRAAAILNRLAPERGVAIEHNL
                     LTRQIEGAQRKVEGRNFDMRKQVLEYDDVANEQRKVIYSQRNEILTSKDISDLMQEIR
                     SDVVSDLVDTYMPPDSMEEQWDIPTLENRLAAEFRLHEDIQSWLKADNAIDGQDIKER
                     LIERIENEYAAKTELVGKQAMADFERNVMLQVIDNQWREHLAAMDYLRQGIHLRSYAQ
                     KNPKQEYKREAFTMFQDLWNGIKFHIASLLTSVQIEQNPVAVVEEQPIGNIQSIHSES
                     PDMEELLGQSQTDLVTEAFNPDGTDFSPEALEARGQIVHRNDPCPCGSGLKYKQCHGK
                     LA"
     gene            26723..26875
                     /locus_tag="WGQ68_000661"
     CDS             26723..26875
                     /locus_tag="WGQ68_000661"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714509.1"
                     /translation="MPSEHPAPVSDGILPEPPHPTAIPKNPDFVPSVPKTDIPSAPHH
                     DSIRLH"
     gene            26853..28625
                     /locus_tag="WGQ68_000662"
     CDS             26853..28625
                     /locus_tag="WGQ68_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEZ0714510.1"
                     /translation="MIPSDFIDELLAKTDIVDIIDEQVPLKKGGANYMACCPFHKEKT
                     PSFSVSPTKQFYHCFSCGAHGSAIGFVMEHQGLSFPEAVQFLADRVGMVVPKVHGQND
                     NPEVRAERKKKQQTLEETTAAAADFYAQQLKFNPAAKAYLDKRGLSAEVIAHYGLGYA
                     PDGWQPLTQVFQPYPNTALVDTGMVIDNEGRHYDRFRHRIMFPIRNPRGQVIGFGGRV
                     LDDSKPKYLNSPDTPLFDKGKNLYGLYEGRAAVKEAGRILVVEGYMDVVALAQFGVGY
                     GVAALGTATTAEHVKILMRQADSIYFCFDGDSAGRKAAWRALENALPQLKDDKSLHFL
                     FLPEEHDPDSYIRAYGKAQFEDALLNQSKPLSEYFWEHLSDGIHLNTQEGKAELVKTS
                     SPLLAQITAPALAYLLKQRLSELVGIDPDNLAQLLGQEAPKRHVKQKNYKLPPISVKQ
                     PVMLTLVQRQIRSLLINPDWAAYIDLPDYLALDGDFACLANLAESIKNHAAVPETAQV
                     LEYMRGSPYEETITRIFHSTHQSEEMNSSSEEDCENFQIGMKKLLNELKYSQIETLKQ
                     KSLQSGLNESEKKLLLSLLTAKQN"
     gene            28812..30740
                     /gene="rpoD"
                     /locus_tag="WGQ68_000663"
     CDS             28812..30740
                     /gene="rpoD"
                     /locus_tag="WGQ68_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEZ0714511.1"
                     /translation="MSRNQNHEEYQDDTRPLSIEEQRARLRQLIIMGKERGYITYSEI
                     NDALPDDMSDADQIDNIVSMISGLGIQVTEHAPDAEDILLSDNAAVTDDDAVEEAEAA
                     LSSADSEFGRTTDPVRMYMREMGQVDLLTREDEIIIAKKIENALKNMVQAISACPGSI
                     AEILELIEKIRKDEIRVDEVVEAIIDPNEVLLNELGLGHLETTAPEKPSNDNSDENED
                     DEESEEDADEISAANLAELKQKVIGHFAQIEKDYKKMIGRLEKHHSRHKDYLAYRDAI
                     ANKLLEVRFATRQIDSLSSSLRGKVENIRKLEREIRDICLDRVHMERDYFIQNFLPEI
                     TNLEWIEEEIAKGRVWSDALDRFRHAILEKQTELADMEKETRISIEELKEINKNMVSS
                     EKETAAAKQEMIQANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYK
                     FSTYATWWIRQAITRSIADQARTIRIPVHMIETINKMNRISRQHLQETGEEPDSAKLA
                     ELMQMPEDKIRKIMKIAKEPISMETPIGDDDDSHLGDFIEDANNVAPADAAMYTSLHE
                     VTKEILESLTPREAKVLRMRFGIDMNTDHTLEEVGRQFDVTRERIRQIEAKALRKLRH
                     PTRSDRLRSFLDSEDSKL"
     gene            30887..>31704
                     /locus_tag="WGQ68_000664"
     CDS             30887..>31704
                     /locus_tag="WGQ68_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0714512.1"
                     /translation="MSTFFRQTAQAMIARHIDRFPLLKLDQVIDWQPIEQYLNRQRIR
                     YVRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYS
                     TLCRYRNWLAQDDTLSELLKLINRQLTEKGLKIEKASAAVIDATIIQTAGSKQRQAIE
                     VDEEGQISGQTTPSKDSDARWIKKNGLYRLGYKQHTRTDAEGYIEKLHITPANTHECN
                     HLLPLLEGITEGTTIYADKGYDSAENRTSGRTSVVRRHYARAAYFGLIKVSAQS"
BASE COUNT         8112 a         9172 c         7490 g         6930 t
ORIGIN      
        1 gtccttgtgg gaatgacggg atgtaggttc gtaggaatga cgtggtgcag gtttccgtat
       61 ggatggattc gtcgttcccg cgaaagcggg aatccggaac cagaaaccac aaaaatttat
      121 cgggaaaacc gaaactcaaa aacaacggaa accgaacgga caggattccc gcctgcgcgg
      181 gaatgacggc tcatacatca cccaaaaaat tgaaaccaaa cagatgggat tccctcttgc
      241 gtgaggctga cagatgccgt ctgaaagact ttcagacggc atagcttttt ctctttgaat
      301 ttatagtgga ttaacaaaaa tcaggacaag gcggcgagcc gcagacagta cagatagtac
      361 ggaaccgatt cactcggtgc ttcagcacct tagagaatcg ttctctttga gctaaggcga
      421 ggcaacgccg tactggtttt tgttaatcca cgataaattt gccacaaaaa agctgcctca
      481 aatgaatacc cgggcagctt tttgttgata tgactccaat cagcggtgtt gcggattgta
      541 acgtttttcc aaacgcagga atatccagcc taagaaagtc gtcatcaaca gataaatcag
      601 ggcgacggtg taaagcggtt cttcataaac cgaataccgg cccgtaatcg tattctgaac
      661 atacgccaac tccgccacag caatgaccga cagcagcgag ctgtctttca agagcgtgat
      721 gaactcgctc gccaaaggcg gcagcatgcg gcgcaatgcc tgcggcagaa tcacatagcg
      781 catcgcctgc ggataggtca gccccaaaga acgcgccgcc tccatctgtc ctttgtctat
      841 agactggatg cccgcgcgga aaatctcaca gatatacgcc cccgagttgg cgatcagtgc
      901 caaagaaccg gcaatcagcg gcccgtatcc gcgacgcagc gcgattgccg cctcgccgct
      961 gaccaaaatg ccgtctgaag gatggacgaa aaacggaaac cacacatacg cccaaatcac
     1021 aatctgcaca aacagcggcg taccccggaa cagcgtaaca tacagcagcg aaactttacg
     1081 caacgcccac gccagcacgc gcatcggcgc accggctttt tccaagtgaa tcaggcgcgc
     1141 caacgccaac aacagaccca ataccgaacc gcccgccgtc gccacgaccg tcagccccaa
     1201 ggtcgtcagt gcgccgtaaa ggaacatcca gcggtattcg taaataatgt caaaacgaaa
     1261 atccatatac cgtccgtatc aaaaaccggc ggaactgccg ccgttgcaaa ataatccgcc
     1321 attttaccgt aaaaaccgcc gcctgaactt ttttatcgcg gcagacggcg gttgcgcgtc
     1381 tccgcaaaaa tgcagggcgc gcggttttca gacggcattt gccgttcaaa gccgtgcggt
     1441 gtctttacca aatgcccaac cattcgccca cggcatccat ccaatcctta ttgcccccgc
     1501 cgctcctgcc tgcccggcgg tacgcccacg gcgcttgcgg atttttagct ttccacaatc
     1561 ctttgcgttc cctttccgcc tgaatttgag cgtcggcata atcggcaaaa tccgccttat
     1621 cctgctgttc tttagcataa cttttataat gccacgccgc cccgtcctgc acctgcatca
     1681 ggttcaaatc ggttttgccg acagaaacct gcgccacttc gcgctggtag cggtcggtgt
     1741 cgaacacgcg cacgctgact ttcctgcctt ccgccgccgc acgcaggttg tcgcgcgaac
     1801 gcgtgccgta agcctgtttc atctccggcg cgtcgatata cgccatccgg attttgtgtt
     1861 tcgcgccgtc gccgtcgata acgtgaaggg tgtcgccgtc atagactttg gacaccgtgc
     1921 ctgtgtagcg gtggccggat ttcgccgatg ctcggcggcg ggcgggcgcg tcggaacccg
     1981 cgtcccctgc cgcgccgagt acgtcgagta cggcaaccgc cgtccgcacc gcctcgctgc
     2041 cgtaccccgt ataacccaac gcacccaaaa gcgacagggc gacgggaagc catttcatga
     2101 tttttttaat ctgcatattt ttcaaatgcc gatgccgtct gaacatctct gaaccggatt
     2161 tcagacggca tcttaacgtc aggattaccc ttggcaggga tagatgactt tcgcaccctc
     2221 ttccgtcccc aaaatcaaca catcggcggc atcgcgggcg aatattccgt tttcgagtac
     2281 gccggtgatt ttgttgattt cgtcttccat catcagcggt ttgtcgatat tcaagccgtg
     2341 gacatcgacg atttggttgc cgtaaaaagt ggtgtagccg atacgcaatt caggctgtcc
     2401 gcccatagcg agcagtttgc gcgaaacaag ggaacgcgcg ctttcgacga cttccacggg
     2461 cagagggaat ttgcccaaac gtgaaacata tttgctttcg tccgcaatgc agacgaattt
     2521 ttctgatgcg ctggcgacga ttttttcgtt gaggtgtgcg ccgccgccgc ctttaatcat
     2581 ttgcagggcg tggttcactt catccgcacc gtcaatatag accgccaacc ccgatacttc
     2641 gttcagggaa acgacgggaa tatcatattg ggcaagcagt tcactggatt ttttggaagt
     2701 agataccgcg cctttgattt ttttgccgct cttacccaag gcttcgatga aaaagttgat
     2761 ggtcgagccg gtaccgatgc cgatgtattc gttttcgggt acgaattcga ctgctttttc
     2821 ggcggcgatg cgcttgagtt cgtcttgtgt cgtcatattt ttgtcctttg ggaaaccgta
     2881 tcaacaaaca gccgccatct taacattttt ttgcacgtcc tgcccgccgc gttcaaatcc
     2941 gtaccagcaa taccgccgcc tgcgcctcta tgccttccat gcgcccgaga taaccgagtt
     3001 tttcgttggt tttgcctttg atgttgacgc acgaaatgtc tatgcccaaa tcggcggcga
     3061 tgttggcacg catttgcgga atatgcggcg cgagtttggg tttctgtgca atcacggtcg
     3121 tatcgacatt gaccgcctgc caaccctgcg cctgaacgct ttgatacgcc gcacgcaaaa
     3181 ggacgcggct gtccgcatct ttgaactctg cggcggtgtc ggggaaatgg ctgccgatat
     3241 cgcccaaacc tgccgcaccg agcagcgcgt cggtaacagc gtgcagcagc gcatcggcat
     3301 cggagtgtcc gagcagccct ttttcaaatg ggatttcaac tccgccaagt atcagctttc
     3361 tgccttcggt cagttggtgg acatcgtagc cctgtccgat acggatgttc gtcatcgttt
     3421 gtgttcctga tgttttgaat tgaagttcag acggcattga gcagcagcct gacgatgtat
     3481 gcgtcctgcg gctgcgtcag tttcaaattg cgcgcgtcgc cctgcaccag caaagggcgg
     3541 atgcccaatt tttccacggc ggacgcttca tcggtaatgc cgtccaagtt ttccgcagcc
     3601 aatgcgcggt gcagcagccc cgcgcggaaa agctgcggcg tttgtgcctg ccaaaggctc
     3661 gtccgctcga cagtggcact gatttgtccg ccttctgcgc gtttgagcgt atcggcaatg
     3721 gaaattgcca aaatcccgcc ttcggcggcg ttgcccgcct gttctatcaa ccgcgtcaaa
     3781 gcttcagacg gcaggcagca gcgcgcggca tcgtgtacca gaatattgtc atccgccgat
     3841 gccaagccga tttccaacag ttttgccaca ccgtttcgga cggtttcggc gcgggtctgt
     3901 ccgccgtttt tccacacccg aacctgtgga aatgccgtct gaaccttatc ggcaaacgtg
     3961 tcttcgggcg agacgacaac gacggtcaaa tcgacggcct catgccgttc aaaaatccca
     4021 atcgtatgtt ctaaaacggt tttgcttccg atttcgacat attgcttggg tttgtccgca
     4081 ccgaaacgcg ccccgatgcc ggcggcggga atcagcgcga tatttttgcg cttcatgcgt
     4141 ccgtcccgcc gttttcagac ggcacggctt ccttgcgcca gatacaggct tcgcccaagc
     4201 cgtccaaata ttgcccgtgc gccgccaact cgttttcgtc cgccctgatg actttcagtt
     4261 tgccgctgcg tttggtttcg gtatgcacca cgggtttggt ttccattttt tcctctgcgg
     4321 cctcccccat caggtcgaac tgccgccgcg tcatagcaag atagacttcg cccaaaagtt
     4381 cgcagtcgat caatgcgccg tgcaggacgc gcttgctgcg gtcgacggaa aaacggttgc
     4441 acagggcatc caggctggct ttctgcccag ggaacatttc gcgcgccatc gccagggtat
     4501 cggtaacggt acagccgagt tcctcgacag tcggcaaccc catccggcgg aactccatat
     4561 tgaggaagcc cacgtcgaat ttggcattgt ggataatcaa ctccgcaccg cgcaggaaat
     4621 cggcaatctg cctgccgacc tctgcaaacg gcggcgcgtt tttcccttcc aaaacctcta
     4681 tcgtcagacc gtgcaccctc gccgcctctt cgggcatatc gcgctcgggg tggacataga
     4741 ggtgcaggtt tttgtcggtc atttggcggt tgaccatttc taaaccggca aactcgacca
     4801 agcggtcgcc gccgtcggca tacaggccgg tggtttcggt atcgaggatg atttggcgtg
     4861 tcgtcatatc ggtgtctttc ttctatcttc gtaaattgat tattttttaa gcaatgtatt
     4921 tttctgtttt catttcaatg cacaaaccca cttattcaca gtgtgttcac aacattgggc
     4981 aggcggattg tgtattttgg ggacaatttt ttcagacggc attcaaggtt ttttcctgat
     5041 tgccgccgcg cctaaaaacc gcctttcgcg cttaatcaaa aataccgaca acggaatatt
     5101 gcccaaagcg acaatcagat acaacaagga aatgctgtca aacacaaaca gcaacaccgc
     5161 gctcaaaacg gcggcggaaa ccataaaaat accgttaacg atattgttgg cggcaacggc
     5221 gcgggcgcgg aaagtttcgc tgctggcggt ttgcagccag gtatagagcg gaacggagaa
     5281 aaatccgccg aaaaagccga tcagcatcat caccgccatc acggggtatg cccatccttg
     5341 cgataaaaac caaaaaatgc cgttcagccc ttcaaaacgg tgtccgtgcg tcagccacac
     5401 caaaaccaga ccgcaaaccg tcaaacccaa cgcaccaacc gttacccaag ccaacatcag
     5461 ccgttccctg ctgaacttgg cacacagtac cgaaccggcg gcaataccga tggaaaacag
     5521 ggcaagcatc aggttgaaaa cattgtcgtt gccgcccaga tggatttggg taaaggtcgg
     5581 cagttgcgtg gtataaaccg cgccgacaaa ccaaaaccac gaaataccga taatggcggt
     5641 aaaaacgggc ttgtgccgca ccgtttcacg cagcagggat tttgtgccac ggacaatatt
     5701 ccactcaatt tgggtatcgg cagccttggc gggtacagac ggcataaaca ggctgccgac
     5761 cgtgcctccg acggcgacca gcaaaaccag tatcccgaca atataaggcg gtacacctgc
     5821 caccgccgtt cccaaaatct gaccgaacag gatggcgaca aacgtacccg attcaatcag
     5881 gctgttgccc atcatcaact ctttgtcgtc gagataatcg ggcaggatgg cgtatttcag
     5941 cggcccgaac agcgtcgatt gcgcgcccat gcaaaacaga cacgccaaaa gcagcggggc
     6001 agaccggata taaaacccgt atgccgccac cgccataatg atcatttcca gcaccttgac
     6061 ccaacgcgcc aaaacggcct tgtcgaattt gttacccaac tgccccgaca gcgaggaaaa
     6121 caggaaatac ggcaaaataa acaacaacgc gcccaagttc aacatctgtc cggcaggcag
     6181 gaagccgttt tgccccaaac cgtaaaaccc aatcatcaca aacagcgcgg ttttgaacac
     6241 attgtcgttg aacgcgccga gaaactgcgt agcgaaaaga ggtgcgaaac ggcggctttt
     6301 aaccagtccc aaaccgcctt ttttagcgta catcgttttc cctctctttt tcaatcagtt
     6361 tacttgtcga atcatcatcc atcaggatgc ggtgcgccgg cccttccaag tcgtcaaact
     6421 gcccgttttt gcccgaccac caaaaaaacc agccgatgac aaacgccaaa ataatgctga
     6481 tgggcaccaa tataaacatg ctttccatca catattccct gtcaaatcgt tcaaaacaaa
     6541 agtctgcccc gacacggtca gatattcgtt acgcaaagtt ccgacgggag cttcgtcaaa
     6601 aaacagctcg atacggtctt tgatcacgcg ccaatattgg gggatttccg tctgaccgaa
     6661 cggcgacagg acatgatttt ccattccgcc ttcaagtttg acggcaaaac gcccgctttg
     6721 cggccgtgct tccgattcgt cgtcggcaag caggatgaaa aagcctatat gccgtcccga
     6781 ttggtcatga atactgaaat aatgcataaa tttcccaccc gccttttttc agacgacacc
     6841 aactaaaaac agggcgaatg taccagtttg gatgggaaga atgcaaagaa attctccctc
     6901 ccccagccga aaacaccggc aaaccgcata tccccctttt ttccgtcaaa atacccgact
     6961 tccgccattt tcacgcaaac gcccgattaa gccaagcaat tgcaaagatt ttttgttaga
     7021 atagcctgct tcttttatca accttttcag acggccccac tactttcccg cccaggaagg
     7081 caaaacggat tcggcacgaa tccggttagt atccgtgtcc gattccaatg ccgtctgaaa
     7141 ctttccggag taagaaaatg tcccaaaaat tgatcttggt tttgaactgc ggcagctcgt
     7201 ccctcaaagg cgcggtcctg gataacggca gcggcgaagt cctgctcagc tgccttgccg
     7261 aaaaactcaa cctgcccgat gcctacatca cattcaaagt aaacggcgaa aaacacaaag
     7321 tcgatctgtc cgcacatccc gaccacaccg gcgcggtcga agccctgatg gaagaactca
     7381 aagcccacgg cctcgacagc cgcatcggcg ccatcggcca ccgcgtcgtc agcggcggcg
     7441 aactgtacag cgaatccatc ctcgttgacg acgaagtcat tgccggcatc gaaaaatgca
     7501 tcccgctcgc ccccctgcac aaccccgccc acctcttggg cctgcgtgcc gcgcaaagca
     7561 ttttcaaaga cctgcccaac gtcgtcgtat tcgatacctc cttccaccaa accatgcccg
     7621 aagtcgccta caaatacgcc gttccgcagg agttgtatga aaaatacggc ctgcgccgtt
     7681 acggcgcgca cggtaccagc taccgcttcg tcgccgacga aaccgcgcgc ttcctcggca
     7741 aagacaaaaa agacctgcgt atggtcattg cccacttggg caacggcgcg tccatcaccg
     7801 ccgtcgccaa cggcgaatcg cgcgacacca gtatgggcct gaccccgctg gaagggctgg
     7861 taatgggtac gcgcagcggc gacatcgatc cttccgtatt cggcttcctt gccgaaaacg
     7921 ccaatatgac catcgcccaa atcactgaaa tgctgaacaa aaaatccggt ctgctcggca
     7981 tttccggcct gtccaacgac tgccgcacca ttgaagaaga agccgccaag gggcataaag
     8041 gcgcgaaatt ggccttggat atgtttatct accgccttgc caaatacatc ggcagtatgg
     8101 cggttgccgc aggcggtttg gacgcactgg tctttaccgg cggcatcggc gaaaactccg
     8161 acatcatccg cgaacgcgtg atcggctact tgggcttcct cggtctgaac atcgaccaag
     8221 aagccaacct gaaagcccgc ttcggcaacg ccggcgtgat taccactgcc gacagcaaag
     8281 ccgttgccgt ggtcattccg accaacgaag agctgatgat tgcccacgac actgcccgtt
     8341 tgagcggctt gtaaggtttt atccgcacac gaactgcctc tggaaacgga ggcagttttt
     8401 ttatccggct ttccatgctt aaacagcact gcctcttttc agacattgac ggttgcagcc
     8461 gactacctga accttaatga ttgactattt tttaatcatg ttattatttt ccataaaata
     8521 catgacatta agatgttttt ccacaaaaga tacacacacc ggcaaacacc ggctgtgttt
     8581 atcttttctt atgcctattt tttaatcatc gtatttttat cttttaattt caatacgcaa
     8641 actaacttat acacacggtt ttcacatctt tggactgctt ccgtgtgtat agtggatatt
     8701 gccgttttcc tttctgacaa aaatgccgtc tgagaacttc agacggcatt tgaaacatcg
     8761 gaatcagcgg ttttgttcat accactcgat aaacttgtct gctttgacaa aacccagcag
     8821 cggctcgctg cggctgccgt cggagcggac gacaaacacg cccggcggcc cgaacagacc
     8881 gtattctttc aacaacgcct gatgttcggg catgttggcg gttacatcga tttggaaaaa
     8941 gcgttccata tcgactgcct gatgcacttc cggctgattg agcgtgtaag ccgccatttc
     9001 tttgcaggaa atgcaccagt cggcataaaa atccaaaacg acgggtttgt cgggatgttc
     9061 tttcaacgcc gtatccatcg ctgccttcag cgcggcagta tcggcaaaca tttttccgtg
     9121 ttccgaagat ttgcctgctt cagccggcgg attgagggtc aggaaatggt gcagcgcggt
     9181 cgttttgccg tttgcgccct gccagccgaa ccacgcgccg cctatcagca atatgccgcc
     9241 caatacgaat gccacagctt tcggacggcg tttctgcctg cgtccgttga ccagcagcat
     9301 acaggcagga accagcatca gcagcgtgta cagcgcgacg acgagataat agggcaagtg
     9361 cggcgtggcg aggtaaacgg cgacggcaag caggataaag ccgaaagcgt atttgacggc
     9421 attcatccaa tcgcctgcct taggcaggat atgcccgccg aacgtgccga tggcaatcag
     9481 cggaacgccg gtgcccaacg ccaaagtgta aagtgccaaa ccgcctaaaa ccgcatcgcc
     9541 cgtctgaccg atgtagccca aagcaaatgc cagcggcggg gcgacgcacg gcccgacaat
     9601 cagcgcggac aatatgccca taataaagac ggaaacgatt ttaccgcctg aaagcctgct
     9661 gctttgattc tgaaaatacg actgcacggc gttgggaagc tggatgttga acagcccgaa
     9721 catagacagt gccaagacga ccattaaagc cgatgccgcc aacaccaccc aagcctgctg
     9781 caaccatacg gtcagcagtg cgcccgtcag tccggcaaca atgccgacca gcgtataagt
     9841 cagagccaaa ccctgaacat aaacgacgga cagcacaaac gcccgcgcct tgcccgcctt
     9901 tttgtcgccg accacaatac tggaaacaat cggcaacagg ggatacatac aggcggtaaa
     9961 actcagaccc aaaccggcga gaaaaaacgc caaaagattg gcgttgagcg tatcccaaga
    10021 cagcttgaaa cggctgtcgc cgccctcatc ccccttcggg ggcggcagca ccccgctgcc
    10081 gttttgagag gaaggctgca aaaagcggtc tttggcggat gccggttcgt cggtttgcgg
    10141 atggtaagtg ccgttgccgg aaatatcaaa ctcggtatcc acgggcggat agcacacgcc
    10201 ggcttcggca cagccctgat aggtcaaaac caatttatac ggttcgccga cagcctttgc
    10261 ataaggaaag gcaacctgcg cttcgtgatg gtaaaccgtc tgcctgccga aaaactcgtc
    10321 ttccttctct tcacccttgc tgaaagaagg ctgtcccaac aaatccgccg gatcggtctt
    10381 gccgacgatt ttcgcctgat acatatagta tccgtcggca atcctgaaac ggacgttcac
    10441 accgtcgtcg gcaacggcaa gctccggcac gaatgccttt tccggcggca gcagatcgtt
    10501 cgcatccagc gcgaaagctc gtccgcacaa catcaaaaat acggcgaaca ggcaaatcag
    10561 ttttttcata atcgaatccg tttcagacaa ataatttgtc tgcattataa atggtaaggt
    10621 tgacggtggg atttaattta tgtaaaaccc gccattatcc gaacctattt ccataaacat
    10681 cttatcgaac ccgccatgta cgatgtcaat acccacgatg tccgccgctt tttcgcccgc
    10741 gtgtggcagc agcggctcaa tccgctgcaa ctgagcgcac tggaacagaa agccctccgc
    10801 attgtcgaag cccatcccga ataccactgc tatctcgaac gcatcgaaga ccatctggac
    10861 accgactggc tgcccgaaaa cggcgaaagc aaccccttcc tgcatatgtc gctgcatttg
    10921 tccgtccaag aacaggcggg catagaccag ccgcacggca tacgcgcaat ccacgacacc
    10981 ctgtgcgcca aacacggctg gctggaagcc gaacacgaaa tgatggaggc actggcggaa
    11041 acactgtgga cggcgcaacg ctacggcacc ggtttggatg tcaatttcta catgacccga
    11101 ctgcgcaaac tcatcggctt gggtgcagaa aatcaagcca ggttgaaccc gcatgaaatc
    11161 gcctgaccat accaaccgcc tgcaaaatgc cgtctgaagc ggaacaaccc ctttcagacg
    11221 gcattcattt tcccccaatc atttccacaa cgcctttttc agcataatca accaatcctt
    11281 cttatccaaa acggggcgtt gtgcaaacac atcgtatcgg cacgcgtcca gtttctgcaa
    11341 aatcaactgc gcccccaaca caatcatacg gagttccaaa ccgatacgcc ctttcaattc
    11401 gcgcgccaaa ggcgaacccg ccttcagcat acggaatgcg cgccggcact catacgccat
    11461 cagccgctga aacgccgcat ccgccctacc ctccgcaatc tgtccttcgg aaacaccgaa
    11521 tttcaacaaa tcgtcctgcg ggatataaac cctgcccttt tgccaatcca cagccacatc
    11581 ctgccaaaaa ttcaccagtt gcaaagccgt acagataccg tcgctttgcg ccacgcacac
    11641 cgcatccgtt ttcccgtaca aagccagcat aatgcgtccg acagggttgg cggaacgccg
    11701 acaataatcg gccagctcgc cgaaatttcc ataccttgtt ttaaccacat cctgagaaaa
    11761 tgcagaaagc aaatcataaa acggctgcaa atccaaaccg aacggcacaa ccgcctcggc
    11821 atccaatcgt gcaatcaaag gatgcgccga ccggccgccc gatgccaaca cgtccaactc
    11881 gcgctgcaaa ccctccaacc ccgccaacct ggcttcagac ggcatactgc cctcgtccgc
    11941 catatcgtcc gccgtccgtg caaacgcgta caccgcgtga accggcttcc tcaacctgcg
    12001 cggcaaaatc agcgaaccga cgggaaaatt ctcataatgc ccaaccgaca taccttctcc
    12061 atccatcaaa caaaatgccg tctgaaacgg aacaaaccct tttcagacgg catcagatac
    12121 ctccaagctg ccggcaatca gtggtggtga tgaccgtgcg ggccgtggac atgaccgtgt
    12181 gcgatttcct catcggatgc atcgcgcacg ctttcaactg tagccttaaa gcggattttc
    12241 atgcctgcca aaggatggtt gccgtccacc accgccttgc cgtcggcaac atcggttaca
    12301 cgatagacga caacatcgcc agtttcagga tcgtcggctt caaacatcat gccgacttcg
    12361 acttcaacag ggaacacgcc cgcatcttcg atacggacca actccggatc ctgctcgccg
    12421 aacgcatcgt cgggcgacag cgccacatcg accgtatcgc cggcatcctt accgtgcaac
    12481 gcctcttcca ccaaagggaa aatgccgtcg taaccgccgt gcagatacgc aatcggttct
    12541 tcggttttgt ccaaaagctg attgttggca tcatacatct cataatgcag cgaaaccacg
    12601 gaatttttca cgatagccat atttgtcctt tcaggaacag cagattaatt acaggcgcat
    12661 tctaacacaa ccgccgcgcc ggccgattac cgttaacctg ttcataaact gtacagcaca
    12721 tatttcaatg taaatctttg ttattttatt gcggtgtaac ttttttacaa cattcttaaa
    12781 accattccga cctgtctgcc gactttccca atccgcctta ataaatcata caagatactg
    12841 aaattatatt aatctctata atatttatcc ctatcgaatt tttaacagca aaaccgtttt
    12901 acaggattta tcaatccgcc cgccagaaaa cttttcattc aaaccttttt cccatctgta
    12961 cgacattgca atcccttatt ccatagtgca taattacgca aattcagcga tgaatttcca
    13021 acccggtttg tagtatggtc gataaagacc tatttgtttc aataatttaa attggttcta
    13081 aaggttacta aaatgaaaaa atccctgttt gccgctgctt tgttgtcttt ggttctggca
    13141 gcctgcggcg gtgaaaaagc cgctgaagct cccgctgctg aagcacctgc cgccgaagct
    13201 cccgctactg aagcacctgc cgccgaagct cccgctactg aagcacctgc cgccgaagct
    13261 cccgctgctg aagcacctgc cgccgaagct cctgctactg aagcacctgc cgctgaagct
    13321 gccgctaccg aagcacctgc cgctgaagct cctgctgccg aagctgcaaa ataagcattt
    13381 tccgcttgca aaaaagcagg atacgttcag tatcctgctt ttttgatttt tcagacggca
    13441 tcagattccc ttcctcaatc ttctccctac ccttccgaca aacatgcctg accttcatac
    13501 cgaattttcc cgactcctgc cggcagatga aattgccgaa ctttctccga cgcttttaaa
    13561 agaccagcgc aaccgcttta cgtctgcacc agacatcatt ttgcagccgc gcagcgttga
    13621 aagcgtgcaa accattatgc gtttttgtta cgaacaccgc attccggtta cgccgcaagg
    13681 cggcaatacc ggtttgtgcg gcgcggcagt atcggaaaac ggcgtattgc tgaacctttc
    13741 caaactcaac cgcatccgca gcatcaattt gtcagacaac tgcataaccg tcgaagcagg
    13801 ttccgtactc caaaccgtcc aacaggcagc cgaagcctca aacaggctgt tcccactcag
    13861 tctcgccagc gaaggctcct gtcaaatcgg cggcaacatc gcctgcaatg ccggaggttt
    13921 gaacgtattg cgttacggca cgatgcgcga cctggttatc ggtttggaag tcgtcctccc
    13981 caacggcgaa ctggtttccc atctccatcc cctgcataaa aacaccaccg gctacgacct
    14041 gcgccatctg tttatcggca gcgaaggcac actgggcatc atcactgccg ccacgctcaa
    14101 gctttttgcc cgtccctcgg acaaggcaac ggcatgggtc ggcattcccg acatcgaatc
    14161 cgccgtccgc ctgctgaccg aaacccaagc tcactttgcc gaacgcctat gcagttttga
    14221 gctgatcggc cgttttgccg ccgagttgtc ttccgaattc agcaaactcc ccctgccgac
    14281 acattcagaa tggcatattt tacttgagtt gaccgactca ttacccgaca gcaatcttga
    14341 tgatcggctt gtcgaatttc tttataaaaa aggatttacc gacagcgtat tggcacaaag
    14401 cgaacaagaa cgtatccata tgtgggcatt gcgcgaaaac atctccgcat cgcaacgcaa
    14461 actgggcacc agcatcaaac acgatattgc cattcctatc ggacgcgttg ccgactttgt
    14521 ccgccggtgc gccaaagatt tggaacagaa ttttaaaggc atacaaatcg tctgcttcgg
    14581 acatcttggc gacggcagcc tgcactacaa tactttcctg cccgaaatcc tcagcaatga
    14641 agtctatcgt tacgaaaacg acatcaacga cgcagtttat cgcaacgttc ttgcctgcaa
    14701 cggcacgatt gccgccgaac acggcatagg tatcatcaaa aaacagtggc tggacaaagt
    14761 acgcacgcct gccgaaatcg ccctgatgaa aagcatcaaa caacaccttg atccatataa
    14821 cattatgaat ccgggcaaac tgcttccgta aataactgat tcaaaatgaa attataggaa
    14881 atatcatggg aatgacagca acctatcaac tggtcgataa tgaaaaactg gctttgctta
    14941 aaaacaccga taccgaatgg caagaattgt ttgaagaact ggaagatttg gaggatgagg
    15001 ctgccgtctg cctcgacatc gataaaatgt gggatgtttt gcattttgta ctgaccggaa
    15061 caagtgcaga aaatccctta aagggcaatc tgttatccga atcaatcgtc ggaacggcac
    15121 ccatttcgga cgacgacgga tacgccgctt atatcgaatc cgcccaaatc catgaaatca
    15181 tctcagcttt ggaaggcttc gacataaaaa gcgcaatgga gcatttcagt atgcaggtgt
    15241 gcaaagagca tcatatctac cccgacattt gggattatga agatgaaatt tatgatataa
    15301 aagaagaaat taccgactac ttttacaaaa tcggcaattt ttacaaaacc gcccggcatt
    15361 caaatagcaa tgtactggtc agcatttact aaatatattt caaacatcac ggaaacttta
    15421 atgaagacaa agtacggaat cgcactcaag gaaacctagg tatttcaacc tgggagacca
    15481 tcatgagtaa aagttacaga aaacacccct tttcccctat aaccacggca gtcagcgaaa
    15541 aacaggacaa acgccttgtc aaccggaaac tacggagaat agcgcgcgaa tgtctgaaac
    15601 aaggaaaaga accaccccac catatcaggg cagtcagcaa cgtttacgat ttcgccaaag
    15661 acgggcattt ccgtttttcc gcttcccgtc atccgcatct tttacgcaaa tagaaaacag
    15721 ggcaaccgcc ctgtttttcc gtttcgtatt tcaacaccat tgttcaaaca accgtcagac
    15781 ggcattgttc aaacgccgtc tgaaaccgca tcccccccat cagcgcgtat gcttaatcaa
    15841 ccgctgtttc tcgcgtttcc aatccgcctc tttcatactc tgacgtttgt cgtgctgttt
    15901 cttacctttt gccaaaccga tttccatctt gatttttccg cgcgaaaaat gcaaatccag
    15961 cggcacgatg gtgtagccgg cacgttcggt tttgccgatt aatttgttga tttccgactg
    16021 gttcaacaag agcttgcgcg gacgtacggc atctggttta atgtgtgtcg aggctgtggg
    16081 caaagccgta atatggcagc cgaccagata aaacgcgtct tttttccaat agatataact
    16141 ctctttaagc tgtacgcgcg cggcgcggat tgctttgact tcccagcctt ccaagaccaa
    16201 accggcttca atccggtctt caatgaaaaa atcgtgaaat gcttttttat tgttcgcaat
    16261 agccataaac atcctatcaa tatccgccgt cagacggcat aaacccgaaa acagaaccca
    16321 tcataccgcc tcttcaaccg cctgcacaat cttctcggga tacagcctgt tgaggcagtc
    16381 ggtatgcccc agcggacatt cccgcttaaa acagggcgaa cattccaagt gcaggctgac
    16441 gattttcgcc ctgtcgctca aaggcggcgt atgcgtcggg ctggaagaac cgtaaaccgc
    16501 caccaccttc ctgcccaatg ccgccgccaa gtgcatcagc ccgctgtcgt tgcacacgac
    16561 cgtgtccgcc aacgacaaca aatccattgc ctgcgacaaa tcggttttgc cgcacaaatt
    16621 gacacacata ccgtctgaaa ggcagttgat ttcctcggcg atttcatcat ctttttgcga
    16681 accgaacagc caaacctgcc aacccgtcgc cgaataatgt ttgcccaact cggcaaaatg
    16741 ctttgccggc caacgctttg ccggcccgaa ttccgcaccc ggacaaaaag ccagaacagg
    16801 ctttccaaga ttcaaaccga aagtttcgac agaaatttcc cgccgccgtt catcaatgga
    16861 aaactcgggg aatcccgaat gcccgtcaaa atcttcctga ctcggatgcg cgagagccgt
    16921 atatctgtcc accatcaaag gcagacgttc cttatccagc ctgcgtatat cgttcaatag
    16981 aaaataacgg ctttcgccga cataacccgt tctttttccg atgcccgttg ccagcgcgat
    17041 gatggccgat ttcaaagaac cgggcagcac gataacctga tcgtagccgc gccgtcccaa
    17101 atccctaccg acccgccaac ggcgttttaa ctccaacgca ccatgtccga acggattttc
    17161 aagaatctca ttcacttcag gcatacgctc aaacaccgcc atcgaccact tcggcgcgaa
    17221 cacatcaatc gtgcaaccgg ggtgaagttc cttcaaacgg cggaacaagg gctgggtcat
    17281 cacgcagtcg cctatccaac tgggagaaat aatcaggatt ttgatggaca taacaagaaa
    17341 ccgaaatcag acaggcggaa ttttaccgcg aaaccgttgg aaaacctatc tcgccgcatt
    17401 ccgaacgccg gacgtgcaaa tatgaaaaag cccgaacatt caagttcggg cttcaaaatt
    17461 ctggctcccc gacctgggct cgaaccaggg acctgcggat taacagtccg tcgctctacc
    17521 gactgagcta tcggggaatg gggcgtatta tagcgtccgg aaaaaatgtg tcaatcctta
    17581 attttggaaa aatgggcgac aaaacgacaa gcatatgaat cagaaagaca ttaagaccga
    17641 tgccttaaaa ggattgccgt tgtatgaatt tccacagccg tcatcacacc atatttaagc
    17701 ccgatgagcc gttctgccct cccccgctta aaacaatgcc gtctgaactt cgccgcgttc
    17761 caaagccagt aaaaactgtt tgcggttcaa cccgcccgcg tagccggtca gtttgccgtc
    17821 gctgccgatg acgcggtggc agggaatcag gacagacacc ttgttctgcc cgttggcggc
    17881 ggcaacggcg cggacggctt tggggttgcc caaacgctgc gcctgctcct tgtagctgcg
    17941 cgtttcgccg taaggaatcg ccaagagcgc gtcccatgcc tgcttttgaa actcggtgcc
    18001 aatctgctcc aaaggcgtgg caaaggtttt cagacgaccc ttgaagtata agtccaattc
    18061 ctgccgcaaa agttgcgtcc gctcatcctc ccgaaacaca aaccgtccgc gcaaggcttt
    18121 ttggacggcg gcaatttcct gttccaaatg cttctgtccg acaaactcca gcagacacaa
    18181 tcccttattt ccgaacaccg ccagcatctc gcccaaaggc gtggcaatgg cggcacacac
    18241 cagctcattc aaactgtcgg gataacgcgc ttccaacaga cggatggctc ggcggatacg
    18301 gacatattct tcaggcgcgc agccgatatt gtcccaaaaa tcccgatcga actgcccggc
    18361 ttcgcattcc gtcaaattgg gatgcggcat aacgccgcac tcacaaaccc gggtttcgag
    18421 ccaatagcgg atttcatccc attttgacgg cagattgttt aaggaaggca gggtaatcat
    18481 tcgtttgctc cgtatctcta tcatggattt gacggcaaaa ttcccaattt tcgccattcc
    18541 cgcacgctga tgcaggaacg ggctacgacg taaatcttga gggttaggtt gcggcaatgc
    18601 ctaaatatgc gatatttcca aagcctcagg gaaaggaatt aaagacttca gacgacctgc
    18661 aaaggctcgt ctgaaaccac gatttttgca tttgcgcatt ctggcacatc atccaaccgt
    18721 ttcggcacat tcctgccgcc gttgacagcc tataatgaat ccacttattc atcaagcaaa
    18781 ggaatcatct atgcaaaccc gcattctctc cgccgtactg ctggcttttt caaccgctgc
    18841 ctttgccggg ggcgcattca cgctgcaatt cgacaacccg tccgaagacg gcggcttcac
    18901 gcaaaaccag cttttgagcg cgccttacgg atttggctgt tcgggcggca atgcttcgcc
    18961 cgcgctgtcg tggaaaaatc cgcccgccgg gacaaaaagt ttcgtcctga ccgtttacga
    19021 taaagacgcg ccgaccggac tgggctggat gcaccgggtg gtcgccgaca ttcccgccga
    19081 tgtccaccgc cgcaacgcga cctcgctgca attaagccgc tgcgccaaca tcgccgacga
    19141 ccagtccgca gccatatcgg cggtaatcag tttgcggatt tgccgcatca ggttgacgcc
    19201 ttcgtacacg gcaaaaccga tgccgtcatg ctgcaaccac gccaacacgc cgcaaagcgc
    19261 gacctccgcc gcattgtgcg gcacttcttc atccgtcagc accgccgccg cataatcaaa
    19321 cgccgcgccc atccgcccgg ggtgcggcag cttcaccgca tcgcacactg cctgcgccgt
    19381 cccgtattgt gcggcaaacc tttctatggc ttcctgttcg aaagcaatcc attgcgcctg
    19441 atagaggccg tctgaatcgg gaatattaat aacgtcaaac gtctgcccgc ctgccaaggc
    19501 gaccgcctca cccgccgcaa tcctcaattc gcgcctcgcc caccgcagct tcttgcttcc
    19561 gcgccgcacg ataagcacag ccggttcata taccgccacg ctgcggtaca aggcggtatg
    19621 atgttgcacg atgccgccca aagcacccaa tcgttcgcgc gtatgaaagt aatttgacgg
    19681 cactattatc tccaacacgc agggccgcac ataaggcgca gccctgttta ccctgatttg
    19741 gaaagggtta cttcccaaaa aataaaccaa aatcccaccg ccccgaatgg ccgggtttca
    19801 accatattcc gtcggcgtgc ctgaaacttg acccctcccc tgtcggggag ggtcggggag
    19861 agggtaaaac ggggcagata caggcaatat ttccgttgcc accccgatgc cctctcccta
    19921 accctctccc acgggagagg gaatggattg ccgttgaaat aaatcgctct acataaaaaa
    19981 tcaatgtgtt atctcaaacc cacattaggt ctaatcaaat ggtcggatat ccatattcgg
    20041 caagcaagct gctttcagac ggcatttcca gccaacaagc gcgccaatat cccctcatac
    20101 accgcagaca gcttcggaat gtcgtttagc cgcacgtttt cgttgatttg gtggatggtg
    20161 gcattggacg ggcctaattc gatgagttct tttgcaatgg ctttgatgaa gcgtccgtcc
    20221 gaagtgccgc cggtggtgga caattcggcc tcaatgccgc aggtttcggc aatggctgcg
    20281 cgtgccacgt cggtcagttt gcccgcttgg gtcagaaagg gctgccccga acacgaccac
    20341 tgtaaatcgt attgcacgcc gtgtttgtcc aaaatggcgt gaacgcgttg tttcagccct
    20401 gcttcggtgg actcggtgga gaagcggaaa ttgaatttga cattcagctc gcccggaatg
    20461 acgttggtcg cgcctgtacc gccgttgata ttggaaattt gaaagctggt cggcgggaag
    20521 tatttgttgc cttcgtccca gacttcctgc gtcagctcta acaaggccgg ggcaaaagta
    20581 tgcacgggat tgattgccaa atgcggatag gcaatatggc cttgcttgcc tttgacggtc
    20641 aggttgcccg acagcgagcc gcgccggccg tttttaatca tatcgcccaa tttgtccacg
    20701 gctgtcggtt cgccgacgat gcagtagtca atcaactcgc cgcgcgcttt caatacatcg
    20761 acgactttgg tcgtgccgtc caacgcgtcg ccctcttcgt cggaagtaat cagaagcgca
    20821 atgctgcctt ggtggtcggg atgttcggca acgaagcgtt cacaggcggt aacgaagcag
    20881 gcgatgctgg ttttcatgtc cgccgcgccg cgcccgtata atcttccgtc gcgttcggtc
    20941 ggctcgaacg ggggcgaatc ccatttttca acagggcctg tcggtacaac gtcggtatgc
    21001 cctgcaaaac agacgacggg aactttcgtg ccgcgtcgca accagatgtt tttggtgtcg
    21061 ccgaaatgga gttcttcagc cgcaaaaccg attttgtaca ggcgttcgac aagcagtttt
    21121 tggcaatctc ggtcgtcggg ggtaacggat gggcgggaaa tcaacgcttt ggctagttct
    21181 agggattggg tttcggtcat atttgttcac ttttgaaatt agaccatctg aaacgttctg
    21241 aatgtgattt tcagacggca tttaggttag gttggcatac ggggtgggta tttttacccg
    21301 tcagtcttct gaatcatttg ccgtgacacg cttcgtaaag aggcagcaaa tcttccaccg
    21361 tttccgctat ccatttcgcg acatcctgcc tgcccaaatc gtcgcgttcg atgtgtttgc
    21421 cgatgcagaa aaagtcttcg tcgttttgca actttcggtc ggactcgttt tgttgcgcga
    21481 cggtgcggta gtcgtcgtat tcgctttccg caccgtgcca catatcgaaa gaagcgtatt
    21541 tttcggtatc aaaattatcc aaccagcggt tgtaatcagg cagcgcgatg ggcgacacgt
    21601 cggctttgta gcagtgccaa tccaagctga cgctcagtcg gcagcggttg agcagtatcg
    21661 acaaaatcgc ggcggaattt ttatattgtt cgtatttgaa gtaggcaaag aaatgggcgc
    21721 gaacctgcca gccgttacac cagcgttcga tgtgcggcgg cgcaaacggc gcacccaatt
    21781 cggcggcaac ctgctgaatc agctgctgcc atatctgcca gttttcttta tagtcagcct
    21841 tgatttgcgg aatgctttca ggctggtatt ttttaagctg ggaaaattgg aaaaacggga
    21901 tattgaacaa atcgcaactt ttcggggtca gcataatata tccttgagac gattgtttca
    21961 gacggcatta tttgcgccgg cgcgccgcca taatttcgcc gatttcggtc agtttttctt
    22021 ttgggataaa ggtgttgccc atatcaaaca gcggctcttc aatcgccaaa tgaacatcat
    22081 atcccgccac aaaacgtttg aacgcttcct catcggggac ataagcgttg tctgcttcga
    22141 gtttggcaaa ttcggcggaa acagccgccc agttgtcgtg cagcccgata tgttggcgca
    22201 aaagctcgtc cacgctttct tgggcttgcg gcgcatattg cagcagcagc gggaagaagt
    22261 tttcttcttc gtcttcatgg tgcagcggcg cggcaacgtt gaaatactgg gcgatttggc
    22321 ggatggtttg caaaacaagc tgattgcagc cgttttcggc gatgtagtcc gacagcatcg
    22381 cgacttggcc gcaaaaacgg cgtactttgc cgtggcaggc atacagcatt tcaatcggtt
    22441 cggcaaaggt aacgctttgg gtttcaaaca gattcatgtt ttcgttctca acggggcact
    22501 tttcaagcag tcattttata ataaaacagc ctgcacaaag caggctgtcc gtcttttgag
    22561 actttaagcg gattaatcga ccaaagtcac tttgccgttc atcaaagcac cgtgacccgg
    22621 gaaggtgcag gcaaatttgt attcgccgtc agccagtttg gcaggatcca gagtcaggga
    22681 agcttcttcg ccgccgccga tcagtttggt atgggcaaca acgcgtgcat catcaggttt
    22741 aacataatcg gtatcggcag cacctacgcc gtctttaaat acgccgtcca tatcttcagc
    22801 tttggcaatc acgagattgt gacccatgct ggttttgggt tgcgtaccgg tatgtttcag
    22861 agtgatggtg aactctttac atgctttgct gacttggatg tctttggtgt tgaactgcat
    22921 attgtcgttg gattcgacag ttgccgcaca gttgccggca gcaggggctt cggcagcatc
    22981 tgcaggagca gcttcggcgg caggcgcttc ggaagcgggt gcttcagcag caggagttgc
    23041 ctcggcagca ggcgcggcag gttcttgaga gcaggcagcc aaaccgataa cggcggcaga
    23101 aatcagagcc agatacgctt tcataacaaa tctccaatcg ataaaataat attcggtttt
    23161 acagaaatca aagtgcaacc gccattaaca aaaccttgaa aaagattccg ccgcgttgca
    23221 caaacagatg tttcggagcg gcattttgct acaaatttca tttgaaatca aagcctgtct
    23281 gcaagtttac aatcgtttac tttaaaaggg gcaattttac cccgaaccta tttctttagt
    23341 attagaccca ttatccttta cttcttaata ttaacggatg tttacacaaa ttcccgtata
    23401 cattttatgc gccatgcctt ctaaccaagt ttgccaatgc ctccgccaat tcgggatgcc
    23461 gtttttccaa ctttaccgcc gccgaaccga aactctccag cgcagcctta ctcaaatgca
    23521 gggtattggt tttcggcggt ttttccggtt tcgggaccag cctgaccgaa acagagcgta
    23581 tcgaagcgtc aagccctgcc aactgcggca ataccgacgg tgcaatcatt ttcaagcgcg
    23641 atgccgccat attgtttgcc gccaaaagga caagcctgcc gtcttcgata catgccgtct
    23701 gaaaatgcgg gtgcaggttg gcaggcagca gttttttcac ggcggcatcc aaccgccgcc
    23761 actgtcccgc ctgtttcaaa agtccggaaa gcagcgcgtc ccgcctgccc aactgttcca
    23821 aattcataaa acatacaccc aaaaagattg aaatacctca aacgcgcctt tatttcagac
    23881 ggcattagca ctttgcacaa acgcttgtgt taaaatcgcg ttttcgccca ctattatatc
    23941 aggcgcagga attattcatg ctgacaaaca ttgccaagaa aatcttcggc agccgcaacg
    24001 accgcttgct gaaacaatac cgtaaatccg ttgccagaat caacgcgctc gaagaacaga
    24061 tgcaagccct aagcgatgct gatctgcaag ccaaaactgc cgaattcaaa caacgcctcg
    24121 ccgacggtca gactttggac ggcattttgc ccgaagcctt cgccgtctgc cgcgaagcgt
    24181 cccgccgcac cctcggtatg cgccacttcg acgtgcagct tatcggcggt atggtgctgc
    24241 acgacggcaa aatcgccgaa atgcgtaccg gcgaaggcaa aaccctggtc gccaccctcg
    24301 ccgtctatct caacgcgctg gccggcaaag gcgtacacgt cgttaccgtc aacgactacc
    24361 tcgcctcacg cgatgcgggc attatggagc cgctctataa tttcctcggt ctgaccgtcg
    24421 gcgtgattat ttcagatatg cagccgttcg accgtcaaaa cgcctatgcc gccgatatca
    24481 cctacggcac caataatgaa ttcggcttcg actacctgcg cgacaatatg gttaccgacc
    24541 aatacgacaa agtgcagcgc gaattgaatt ttgccgttgt cgatgaagtg gattccatct
    24601 tgattgacga agcgcgcact ccgctgatta tctccggtca ggcggatgac aacatccagt
    24661 tgtaccaaat catgaacacc gttccgcccc acctcgtccg tcaagagaca gaagaaggcg
    24721 aaggcgacta ttgggtcgac gaaaaggcac atcaggtcat cctgagcgaa gcaggtcacg
    24781 aacacgccga gcaaatcctg acccaaatgg gattgctggc agaaaacgac tccctctatt
    24841 ccgccgccaa tatcgccctg atgcaccacc ttatggcggc attgcgcgcg cattccctct
    24901 tccacaaaga ccaacattac gtcatccaag acggcgaaat cgtcatcgtg gacgaattca
    24961 ccggccggct gatgtccggc cgccgctggt cggagggtct gcatcaagcc gtcgaagcca
    25021 aagaaggcgt ggaaatcaaa cgcgaaaacc aaacccttgc ctccatcacc ttccaaaact
    25081 atttccgcct gtacaccaag ctctccggca tgaccggcac agccgatacc gaagctttcg
    25141 agttccaaag catctacaac ctcgaaaccg tcatcatccc gaccaaccgc cctgtacagc
    25201 gcaaagactt caacgaccag attttccgtt ccgccgaaga aaaattcgaa gccgtcgtca
    25261 aagacatcga agaatgccac aaacgtgggc agcccgtcct cgtcggcacc accagcatcg
    25321 aaaactccga actggtctcc caccttctgc aaaaagccgg actgccgcac aacgtcctca
    25381 acgccaaaga acacgaacgc gaagccctga ttgtcgccca agccggcaaa gtcggcgcga
    25441 ttaccgttgc caccaatatg gcgggacgcg gtacggacat cgttttaggc ggcaacctga
    25501 agcaccaaac cgatgccatc cgcgccgacg aaaccttgag cgacgaagag aaacaggcac
    25561 aaatcgccgc actcgaagac ggctggcagg cggaacacga caaagtgatg gaagcaggcg
    25621 gtttgcacat catcggtacg gaacgccacg aaagccgccg catcgacaac caattgcgcg
    25681 gacgttccgg ccgtcagggc gaccccggat ccagccgctt ctatctctcc tttgaagacc
    25741 cattgctgcg cttattcgca ctcgaccgcg ccgccgccat cctcaaccgc ctcgcccccg
    25801 aacgcggcgt cgccatcgaa cacaacctgc tgacgcgcca aatcgaaggg gcgcaacgca
    25861 aagtcgaagg cagaaacttc gatatgcgca aacaggtttt ggaatacgac gacgttgcca
    25921 acgaacagcg caaagtcatt tacagccagc gcaacgaaat tctgaccagc aaagacatca
    25981 gcgacctgat gcaggaaatc cgttctgatg tcgtcagcga cctcgtggat acctatatgc
    26041 cgcccgacag catggaagaa caatgggaca tcccgacttt ggagaaccgt ctggctgccg
    26101 aattcagact gcacgaagac atccaatcct ggctgaaggc ggacaatgcg attgacggtc
    26161 aagacatcaa agaacgcctg atcgaacgca tcgaaaacga atatgccgcc aaaaccgaac
    26221 tggtcggcaa gcaggcaatg gccgatttcg agcgcaacgt gatgttgcag gtcatcgaca
    26281 accaatggcg cgaacacctc gccgctatgg actacctgcg acaaggcata cacctgcgca
    26341 gctatgccca aaaaaatccg aagcaggaat acaaacgtga agcctttacc atgttccaag
    26401 acctgtggaa cggcatcaaa ttccatattg cctccctgct tacctcggtt caaatcgaac
    26461 aaaaccctgt cgcggtggtt gaagagcaac ccatcggcaa catccagtcc atccattccg
    26521 aatcgcccga tatggaagaa cttttgggtc agtcgcaaac cgatctggtt accgaagcct
    26581 ttaatcccga tgggacagat ttcagccccg aagccttgga agcgcggggg caaatcgtcc
    26641 accgcaacga cccctgcccc tgcggcagcg gtttgaaata caaacaatgc cacggcaaac
    26701 tggcttaagc gtttgaacgc aaatgccgtc tgaacatccc gctcccgttt cagacggcat
    26761 tttgcctgaa ccgccacatc cgactgccat tccgaaaaat cccgatttcg taccgtccgt
    26821 accaaaaaca gacatcccgt ccgccccaca tcatgattcc atccgacttc attgacgagc
    26881 ttttagccaa aaccgatatt gtcgatatta tcgacgagca ggttccgctg aaaaaaggcg
    26941 gggcgaacta tatggcgtgt tgcccgttcc acaaggaaaa aacgccgtcg ttttcggtca
    27001 gtccaaccaa gcagttttac cattgtttca gttgcggggc acacggctca gcgattggtt
    27061 ttgtgatgga acatcaggga ctgtcgtttc cggaggcggt tcagttcctt gccgaccgcg
    27121 tgggtatggt cgtgccgaaa gtgcacgggc aaaacgataa tcccgaagtc cgtgccgaac
    27181 gtaagaaaaa acagcagaca ctggaggaaa cgacggctgc ggcagctgat ttttacgcgc
    27241 aacagctaaa attcaatcca gcggcaaaag cttatttgga caagcgcggc ttgagtgcag
    27301 aagttatcgc gcattatggt ttgggctatg cgcccgacgg ctggcagcct ttgacgcaag
    27361 tgttccaacc gtatcctaat accgcgttag tggatacggg gatggtgatt gacaatgagg
    27421 gacggcatta cgaccgcttc cgccatcgga ttatgttccc catccgcaat ccgcgcgggc
    27481 aggttatcgg tttcggcggc agggtgctgg acgactcgaa gccgaaatat ttaaattctc
    27541 ccgatacgcc tttgttcgat aaggggaaaa acctttacgg actgtatgaa gggcgtgccg
    27601 ctgtcaagga agcggggcgg attttggtgg tcgaaggcta tatggacgtg gtcgcgctgg
    27661 cacagttcgg cgtgggctac ggcgtggcgg ctttgggtac ggcgacgacg gcggaacacg
    27721 tcaaaatcct gatgcgtcag gcagacagta tttatttctg tttcgacggc gacagcgcgg
    27781 ggcgaaaagc ggcttggcgc gcgctggaaa acgcgctgcc gcagttgaag gacgacaaat
    27841 cgctgcattt tttgttcctg ccggaagaac acgaccccga cagctacatc cgcgcctacg
    27901 gcaaagcgca atttgaagac gcgcttctga atcaaagcaa gcctttgtcg gagtatttct
    27961 gggaacacct ttcagacggc attcatctca atacgcagga aggcaaggcg gaattggtaa
    28021 aaaccagttc gccgcttttg gcgcagatta ccgcgccggc attggcttat ttgttaaaac
    28081 aacggcttag cgagctggtc ggcatcgacc ccgacaacct cgcgcaactg ctaggacagg
    28141 aagcgccgaa gcggcacgtc aaacaaaaaa actacaaact gcctccgatt tccgtcaaac
    28201 agcccgtcat gctgacgctg gtacagcggc aaatccgcag cctcttgata aatccggatt
    28261 gggctgcata tatagacctg cccgattatc tggcgttgga cggtgatttc gcctgccttg
    28321 ccaatcttgc cgaatcgatt aaaaaccatg ccgccgtacc cgaaaccgct caggttttag
    28381 agtatatgcg cggctcgcct tacgaagaaa cgataacccg aatcttccat tcaacgcacc
    28441 aatcggaaga aatgaacagc agcagtgaag aagattgcga gaatttccaa atcggcatga
    28501 aaaaactgct caatgagtta aaatacagcc aaatcgaaac attaaaacaa aaaagcctgc
    28561 aatccggctt aaatgaaagc gagaaaaaac ttttgctgtc gctgctgacc gcaaaacaaa
    28621 attgaccggc ggattccgcc atccgtaaac cgttatgccg tctgaaaagc attcaccccg
    28681 gctgcaacaa cgacacctgc agaacaccca tccccaaaag ccttcagacg gcatcagagt
    28741 accctactct gccacgcctt caggtgcgtc caaacgcaaa ccgtcggcat cttaccaaca
    28801 gaaagcagac aatgtccaga aaccaaaatc acgaagaata tcaagacgac acccgtccgt
    28861 taagcattga agagcaacgc gcgcgcctgc gtcagctcat catcatgggt aaagaacgcg
    28921 gctacatcac ctactccgaa atcaacgacg ccctgccaga cgatatgtct gatgccgacc
    28981 aaatagacaa tatcgtcagc atgatttccg gtttgggcat ccaagttacc gaacacgccc
    29041 ccgatgcgga agacatattg ttaagcgaca atgccgccgt taccgacgat gatgccgtcg
    29101 aagaagccga ggccgccctt tccagtgcag attccgagtt cggcagaacc accgaccccg
    29161 tccgtatgta tatgcgcgaa atgggacagg tcgacctgct gacccgcgaa gacgaaatca
    29221 tcatcgcaaa aaaaattgaa aacgccctga aaaatatggt tcaggccatc tccgcctgcc
    29281 cgggatccat tgctgaaatc ttagaactca tcgaaaaaat ccgcaaagac gaaatccgcg
    29341 tcgacgaagt cgtagaagcc attatcgacc cgaatgaagt attgctcaac gaattgggct
    29401 tggggcactt ggaaaccaca gcgcccgaga aaccttccaa cgacaattcg gacgaaaacg
    29461 aagacgacga agaatcggaa gaagatgcgg atgaaatctc ggcagccaat ctcgccgaat
    29521 tgaaacaaaa agtcatcggc cactttgccc aaatcgaaaa agactacaaa aaaatgattg
    29581 gccgtttgga aaaacaccac agccggcaca aagactatct cgcctaccgc gacgcgattg
    29641 ccaacaaact gctggaagtc cgtttcgcca cccggcaaat cgacagcctc agcagcagcc
    29701 tgcgcgggaa agtagaaaac atccgcaaac tcgaacgcga aatccgcgac atctgcctcg
    29761 accgcgtcca tatggaacgc gactacttca tccaaaactt cctgcccgaa atcaccaatc
    29821 tagaatggat tgaagaagaa atcgccaaag gcagggtttg gagcgacgcg ctcgaccgct
    29881 tccgccacgc catcctcgaa aaacaaaccg agttggcgga tatggaaaaa gaaacccgca
    29941 tttccatcga agagttgaaa gaaatcaaca aaaatatggt gtcgagcgaa aaagaaaccg
    30001 cagccgccaa acaggaaatg attcaggcaa acttgcgcct cgtgatttcc atcgccaaaa
    30061 aatataccaa ccggggctta caattccttg atctgattca ggaaggcaac atcggtttga
    30121 tgaaagcggt cgataagttc gaataccgca gaggctataa attctccacc tacgcaacct
    30181 ggtggatccg ccaggcaatt acacgctcga ttgccgatca ggcgcgtacc atccgcattc
    30241 cggtacatat gattgaaacc atcaacaaga tgaaccgcat ctcgcgccaa caccttcaag
    30301 aaaccggcga agaacccgat tccgccaaac ttgccgaact gatgcagatg cccgaagaca
    30361 aaatccgcaa aatcatgaaa atcgccaaag agccgatttc gatggaaacc cccatcggcg
    30421 acgacgacga ttcgcacttg ggcgacttca tcgaagatgc caacaatgtt gcgccggccg
    30481 atgcggcaat gtacaccagc ctgcacgaag taaccaaaga aatcctcgaa agcctgacac
    30541 cgcgtgaggc aaaagtcctg cgtatgcgtt tcggcatcga tatgaacacc gaccacacgc
    30601 tggaagaagt cggcagacag tttgacgtaa cgcgcgaacg catccgacaa atcgaggcaa
    30661 aagcactccg caagctgcgg catccgacaa gaagcgaccg tttgagaagt ttcttggaca
    30721 gcgaagacag caagctgtaa accaaaaaac cgcaggtttc aaatacctgc ggttttttct
    30781 tacacaataa acaacgcttc cacatatccc acactcctat cccgagacct ttgcaaaatt
    30841 ccccaaaatc ccctaaattc ccaccaagac atttagggga tttctcatga gcaccttctt
    30901 ccggcaaacc gcccaagcca tgattgccag acacatcgac cgtttccctc tattgaagtt
    30961 ggatcaagtg attgattggc agccgatcga acaatacctg aaccgtcaaa gaatccgtta
    31021 cgtccgagac caccgcggtc gtcccgccta tcccctgttg tccatgttca aagccgtcct
    31081 gctcggacaa tggcacagtc tctccgatcc cgaactcgaa cacagcctca ttacccgcat
    31141 cgatttcaac ctgttttgcc gtttcgacga actgagcatc cccgattaca gcaccttatg
    31201 ccgctaccgc aactggctgg cgcaagacga caccctgtcc gaattgctca aactgattaa
    31261 ccgccaactg accgaaaaag gcctaaaaat agagaaagca tccgccgccg tcattgacgc
    31321 caccattatt cagaccgccg gcagcaaaca gcgccaagcc atagaagtcg atgaagaagg
    31381 acaaatcagc ggccaaacca caccgagtaa ggacagcgat gcccgttgga taaagaaaaa
    31441 cggcctctac agactcggtt acaaacaaca tacccgtacc gatgcggaag gctatatcga
    31501 gaaactgcac atcaccccag ccaataccca tgagtgcaac cacctgttgc ctttgctgga
    31561 aggcattacc gaaggcacga ccatctatgc cgacaaaggc tatgacagtg cggaaaaccg
    31621 aacatctgga agaacatcag ttgttagacg gcattatgcg cgggcagcct atttcggact
    31681 gattaaagtg agtgcgcaaa gcca
//
LOCUS       DAWFHY010000017        29991 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.017, whole genome shotgun sequence.
ACCESSION   DAWFHY010000017 DAWFHY010000000
VERSION     DAWFHY010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 29991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29991
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.017"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            85..792
                     /locus_tag="WGQ68_000665"
     CDS             85..792
                     /locus_tag="WGQ68_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0714513.1"
                     /translation="MVKTADGYKAIAHIRAGDRVLSKDEASGETGYKPVTARYGNPYR
                     ETVYIKVSDGIGKIQTLVSNKIHPFYSQGKWIQAGRLKKGDTLLSESGAKQTVQNITF
                     KQQPLKAYNLTVADWHTYFVKGDRAETEGVWVHNDCPYGEKYRTEVGSYTNTHESGRT
                     YSGKGTRQRSQISGAREARVNNDPHIATDFTPAKNNREAFKDESRRIDAHGGSKSTNN
                     YNRIESPGKKYRKQDGD"
     gene            795..1118
                     /locus_tag="WGQ68_000666"
     CDS             795..1118
                     /locus_tag="WGQ68_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HEZ0714514.1"
                     /translation="MIVDEKSTSDVLKQYQELGFDLTKPMIIEFFIGGSQKNLQSIES
                     RLISYRQDFQISIEQNEFGEMWTCYCSVNIVPSLENILAIETTLFGIAQKNDCNYEGF
                     GSYGN"
     gene            1177..1404
                     /locus_tag="WGQ68_000667"
     CDS             1177..1404
                     /locus_tag="WGQ68_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714515.1"
                     /translation="MDIKFNTLGVILNGVNPEEKFIKIIDDQENTGGFLILLSSNDKF
                     SSFDSYDDWVENLEILKEYLQESHWIIKWVG"
     gene            1531..2073
                     /locus_tag="WGQ68_000668"
     CDS             1531..2073
                     /locus_tag="WGQ68_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0714516.1"
                     /translation="MINHIEKYIGTISHGSKSDSGSQYKLNIAAIPSSPNRDLKTYIT
                     LELSKHDLNYKSRFEILFVCSLKYDENQIFPFLRWLAETIIENKKILLRGQVIYLPRS
                     IVDSTKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAELVEKKGWNAFEQ
                     FLLDNEVDNLSDMNRKPFVW"
     gene            2238..2915
                     /locus_tag="WGQ68_000669"
     CDS             2238..2915
                     /locus_tag="WGQ68_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0714517.1"
                     /translation="MAHIQTGEYVFAKDEASGKTGYKPVTARYGNPYQETVYIEVSDG
                     IGNNQTLISNRIHPFYSGGKWIKAEDLKAGSRLLSESGKTQTVRNIVVKPTPLKAYNL
                     TVADWHTYFVKGNQAETEGVWVHNDCPYDKGNQRYKDASYHGKNDNSVKSRAPTNGQA
                     ALDNSVQVKSTSPRRVGVDKANNEIVVLDKTQTFNNGSAEYHGHVRSWQDLHTDQKNA
                     LKKAGLD"
     gene            2941..3270
                     /locus_tag="WGQ68_000670"
     CDS             2941..3270
                     /locus_tag="WGQ68_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714518.1"
                     /translation="MTDKSKTEKLISSDDKQSVIDGILDMVFNSKAYEVPWISEKLME
                     LSKNKDLDIAGLSLTCFGHLARLHSNIGDYDKVISLLHSKQDDPELQGRAEDALEDIS
                     LFLSENH"
     gene            3687..4088
                     /locus_tag="WGQ68_000671"
     CDS             3687..4088
                     /locus_tag="WGQ68_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714519.1"
                     /translation="MKIKPLQFSNNNHRFYVDKICVTEQDVNILASDRNYELNIEIFI
                     DNIIHFQITDESYKVKFSEYLFENKEKNDWDRNPAINYFFEIIDDSYMDWLKEESFDF
                     FEKKYYKAYIFFFSDSVIEVISSTEPVFYSK"
     gene            4270..4659
                     /locus_tag="WGQ68_000672"
     CDS             4270..4659
                     /locus_tag="WGQ68_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714520.1"
                     /translation="MWKISKENCEDLGFAIVCMFYDAINLSEFKLWLDIVVRDTPIDT
                     IPLYIFDLIDFDKSIGEIYDVIGVVNYGYISNDQKNALTGIAFLRGIDVYDPPISKEK
                     ALKALEKYPEIYQRFQHFFPFVELPLL"
     gene            complement(4700..6157)
                     /locus_tag="WGQ68_000673"
     CDS             complement(4700..6157)
                     /locus_tag="WGQ68_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEZ0714521.1"
                     /translation="MHKILPIAHVLSRLGMLFSFILLIPAALSYAFSDGAYTAFATTA
                     TVTLSGSYIVRLATLRFRRELRPRDGFTLVLMLWLAFAAMAAMPMYLYFPNMGFTDAF
                     FESMSGLTTTGATVIPHVDGLAPSVNFWRHMLNWLGGMGIIVLAVAILPMLGVGGTQL
                     FKAEIPGIDKESKMSPRISQVAKKLWFGYTLITILAAACLHFAGMSWFDAVCHAMATL
                     SLGGFSTHDASIAYYNSPLIEAVIIVFTIVGGINFASHFAALNSRSLKTYWKDEECRT
                     MLLLLSGSILTAALYLWHTGYYAGFTESLRYTAFNFVSIGLANGLANTDFAQWPLLIS
                     LWMFFLANILANSGSTGGGIKTIRALVLFKFSLREMMVLLHPKAVRTVKISGKAIPDR
                     LALTVMSFIFIYFMTVVLFSFLLMASGMEFTTAFTAVIACITNAGPGLGEVGPAGNYA
                     GLDVMQKWICVTAMLLGRLEIFTVFILFTPAYWKK"
     gene            complement(6172..7002)
                     /locus_tag="WGQ68_000674"
     CDS             complement(6172..7002)
                     /locus_tag="WGQ68_000674"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HEZ0714522.1"
                     /translation="MAHYAIGDIQGCFDELTALLGKIGFNHGTDTLWLTGDIVNRGPK
                     SLETLQFCIRHENSVQIVLGNHDLHLLAVGCGEGAPKRSDTIEPILKHPDGKKMLDWL
                     RAQPLLIREGSRVMVHAGILPQWRITKAESLAREAEAELRGKKYVKFFSKMYGNKPAA
                     WDEGLKGYARLRFIVNAFTRMRALTFKNELDFDYKSTVKKMPPYLRPWFKAPDRQNLD
                     RTIIFGHWSSLGYTNADNVISLDTGALWGGQLTAVNLETEEITQVQAAGGIDWKSFAK
                     "
     gene            complement(7080..7469)
                     /locus_tag="WGQ68_000675"
     CDS             complement(7080..7469)
                     /locus_tag="WGQ68_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0714523.1"
                     /translation="MSKTIIHTDKAPAAIGAYSQAVRAGDTVYMSGQIPLDPATMTVV
                     GNGDFRAEARQVFQNLQAIAEAAGGTLADIVKLNAYLTDLGNFAVFNEVMAEFIAEPF
                     PARAAVGVASLPKGVQVEAEAVLVLNA"
     gene            complement(7605..8129)
                     /gene="nspA"
                     /locus_tag="WGQ68_000676"
     CDS             complement(7605..8129)
                     /gene="nspA"
                     /locus_tag="WGQ68_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein NspA"
                     /protein_id="HEZ0714524.1"
                     /translation="MKKALATLIALAIPAAALAEGASGFYVQADAAHAKASSSLGSAK
                     GFSPRISAGYRINDLRFAVDYTRYKNYKAPSTDFKLYSIGASAIYDFDTQSPVKPYLG
                     ARLSLNRASVDLGGSDSFSQTSIGLGVLTGVSYAVTPNVDLDASYRYNYIGKVNTVKN
                     VRSGELSVGVRVKF"
     gene            complement(8300..8494)
                     /locus_tag="WGQ68_000677"
     CDS             complement(8300..8494)
                     /locus_tag="WGQ68_000677"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714525.1"
                     /translation="MENAAASPAGRFRRHSRRRKCPTLKAQTHTKPYACTCNNPTDTA
                     VFSKPFASFTHPPRDAAATI"
     gene            complement(8714..9913)
                     /locus_tag="WGQ68_000678"
     CDS             complement(8714..9913)
                     /locus_tag="WGQ68_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase-like protein"
                     /protein_id="HEZ0714526.1"
                     /translation="MSTFPNRTMHTISFPNRTRLTALPPLSLYIHIPWCIKKCPYCDF
                     NSHSLKNGLPEAAYIDALLTDLQLELPNIWGRPVETVFFGGGTPSLFQAESIDRLLSG
                     VRSLLRLQPEAEITLEANPGTFEIEKFQGFKDAGITRLSIGVQSFNNDMLSRLGRVHN
                     GKEALTAIATALKLFDKINIDLMYALPNQTVRTALDDVQTAIATGATHISAYHLTMEP
                     NTPFGHTPPKGLPQDEAALDIEDAVHGALENAGFIHYETSAFAKPAMRCRHNLNYWQF
                     GDYLGIGAGAHGKISYPDRIERTVRRRHPNDYLALMQSQPSEAVERKTVAAEDLPFEF
                     MMNALRLTDGVPAAMLQERTGVPSAKIMAQIETARQKGLLETDPAVFRPTEKGRLFLN
                     DLLQCFL"
     gene            complement(9946..12471)
                     /gene="ligA"
                     /locus_tag="WGQ68_000679"
     CDS             complement(9946..12471)
                     /gene="ligA"
                     /locus_tag="WGQ68_000679"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEZ0714527.1"
                     /translation="MNPNSKHNTNFTNLLKPSDSDIKQFAAQHIRHLTDLLNRYAYEY
                     YTLDAPSVPDAEYDKLFRELEALERNHPELKLPDSPTQRVGGEPLAGFAEVRHEVPML
                     SLTNAFSPQDENGVFDHAEMYAFDQRVRDGLDGGKPEYVIEPKFDGLAISLLYRDGVL
                     VQAATRGDGTTGEDVTQNIKTVSNIPLRLHGENTPELIEVRGEVLMQKADFAALNKRQ
                     AENGQKPFANPRNAAAGSLRQLDSRITAQRKLHFFPYSVARRQGGFVAEEHIQELAYF
                     QKLGFSLPNGNFGCFKNIDEVLAFYEQMQQKRPELPYEIDGMVVKVNSLAQQHELGFI
                     SRAPRWAIAHKFPAEEALTIVEAIDVQIGRTGAVTPVARLQPVFVGGVTVTNATLHNQ
                     DEVSRKDVRVGDTVVVRRAGDVIPEVVRVIFERRPMRETAVAVSDGIGHRQDDLFAET
                     PSANQTQSVPLHKPYRLPTHCPICRSEIEREEGEAVARCSGGMLCQAQRAQGLIHFAS
                     RKAMDIDGLGEKQIEQLVAQDLVRHFADLYRLDIPTLQKMKETADKTVAESDQMPSEG
                     SSVGASGKHKKQPTKWAENILAGIESGKTPELARFLFALGIRHVGERTAKTLAQAFGT
                     LERVRRAPEPVLACLPDIGTVVAHSIAHFFAQAEQQAMIDELLAAGVAPQTQAVTIPP
                     ARHAEPQRWIARLPDFKISENKAQALWELAGKNIEGLQNDKALPADWQAWRSEPQNAA
                     LLENLKTFFAQMPSEDEAAQGSDGLNEAVAGKTFVLTGTLPTLKRDQAQSLIEAAGGK
                     VSGSVSKKTDYVVAGEAAGSKLEKANALGVSVLSEAELLTLLG"
     gene            complement(12619..13905)
                     /locus_tag="WGQ68_000680"
     CDS             complement(12619..13905)
                     /locus_tag="WGQ68_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HEZ0714528.1"
                     /translation="MIYIVLFLAAVLAVVAYNMYQENQYRKKVRDQFGHSDKDALLNS
                     KTSHVRDGKPSGGPVMMPKPQPAVKKTAKPQDPAMRNLQEQDAVYIAKQKQAKASPFK
                     TEIETALEESGIIGNSAHTVSEPQTGHSAPKPADAPAKPIPVPQTPTKPLITLKELSK
                     VELPWFDVRFDFISYIALTEAKELHALPRLSNRCRYQIVGCTMDDHFQIAEPIPGIRY
                     QAFIVGIQAVSRNGLASQEELSAFNRQVDAFAQSMGGQTLHTDLAAFIEVASALDAFC
                     ARVDQTIAIHLVSPTSISGVELRSAVTGVGFVLEDDGAFHYTDTSGSTMFSICSLNNE
                     PFTNALLDNQSYKGFSMLLDIPHSPAGEKTFDDLFMDLAVRLSGQLNLNLVNDKMEEV
                     STQWLKDVRTYVLARQEEMLKIGIQPGGKTALRLFS"
     gene            complement(14099..14671)
                     /gene="ampD"
                     /locus_tag="WGQ68_000681"
     CDS             complement(14099..14671)
                     /gene="ampD"
                     /locus_tag="WGQ68_000681"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HEZ0714529.1"
                     /translation="MDNHAEAHWQNGWLQSIRHTPSPNFSPRETGETVSLIVLHNISL
                     PPFKYGTDAVEKLFANRLDPDGHPFFSLIHTLRVSSHFLIKRDGETVQFVSCDNMAYH
                     AGVSSFRGREKCNAFSIGIELEGCDFEPFTEAQYRSLEALLDAICRRYPITAVTGHQD
                     IAPGRKTDPGHFFDWRRIRGKGFPVDRNAV"
     gene            14755..15750
                     /gene="mltG"
                     /locus_tag="WGQ68_000682"
     CDS             14755..15750
                     /gene="mltG"
                     /locus_tag="WGQ68_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEZ0714530.1"
                     /translation="MLKKMLKWTAVFLTVSAAVFAALLFVPKDNGRAYRIKIAKNQGI
                     SSVGRKLAEDRIVFSRHVLTAAAYVLGVHNRLHTGTYRLPSEVSAWDILQKMRGGRPD
                     SVTVQIIEGSRFSHMRKVIDATPDIEHDTKGWSNEKLMAEVAPDAFSGNPEGQFFPDS
                     YEIDAGGSDLQIYQTAYKAMQRRLNEAWESRQDGLPYKNPYEMLIMASLVEKETGHEA
                     DRDHVASVFVNRLKIGMRLQTDPSVIYGMGAAYKGKIRKADLRRDTPYNTYTRGGLPP
                     TPIALPGKAALDAAAHPSGEKYLYFVSKMDGTGLSQFSHDLTEHNAAVRKYILKK"
     gene            15810..16430
                     /locus_tag="WGQ68_000683"
     CDS             15810..16430
                     /locus_tag="WGQ68_000683"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEZ0714531.1"
                     /translation="MKPQFITLDGIDGAGKSTNLAVIKAWFERRGLPVLFTREPGGTP
                     VGEALREILLNPETKAGLRAETLMMFAARMQHIEDVILPALSDGIHVVSDRFTDATFA
                     YQGGGRGMPSEDIEILEHWVQGGLRPDLTLLLDVPLEVSMARIGQTREKDRFEQEQAD
                     FFMRVRSVYLNRAAACPERYAIIDSNRSLDEVRNNIEKVLDGHFGC"
     gene            16652..17932
                     /locus_tag="WGQ68_000684"
     CDS             16652..17932
                     /locus_tag="WGQ68_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme"
                     /protein_id="HEZ0714532.1"
                     /translation="MENSLKEAALKFHELPVPGKISVTPTKSLATDKDLALAYSPGVA
                     APCMEIHADPQNAYKYTAKGNLVAVISNGTAVLGLGDIGALAGKPVMEGKGVLFKKFA
                     GVDVFDIEIDEKDPQKLVDIIAALEPTFGGINLEDIKAPECFYIERELRKRCKIPVFH
                     DDQHGTAIITAAAVLNALRFTGRKIEEATLVCSGAGAAAIACLNQLLDLGLKRENVTV
                     CDSKGVIYQTREDKDRMDESKKFYAVEDNGQRVLADAVKGKDIFLGLSGANLLTPEML
                     NTMNEKPIVFAMANPNPEILPPLAKETRPDVVIGTGRSDFPNQVNNVLCFPFIFRGAL
                     DVGATTINEEMKRACVYALADLAMEEVTEEVVAAYGKKFEFGAEYLIPTPFDSRLLPR
                     VATAAAKAAMESGVATRPIADFEAYAAKLGEWKL"
     gene            18014..18284
                     /locus_tag="WGQ68_000685"
                     /pseudo
     CDS             18014..18284
                     /locus_tag="WGQ68_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713445.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18324..19358
                     /locus_tag="WGQ68_000686"
     CDS             18324..19358
                     /locus_tag="WGQ68_000686"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025462052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEZ0714533.1"
                     /translation="MPNCFKFHTVIERHWQKPYPVLSFLLKPLSGLFAKIAAKRRTDF
                     LSGKRQSEKLPVPVVVVGNIHAGGTGKTPIVAALVSGLQEKGVKVGIISRGYGRKSKA
                     VHVLNAESRAEDAGDEPLLLFRKTGAPTAVGSSRAEAGRALLAAHPDIGLIVADDGLQ
                     HYALRRDVEIAVFPAADTGRTDLDLLPNGSLREPLSRLDSVDTVVVSGGKADALFRPS
                     ENMFHSRIEVGRIYRLNNPSEILDTGRLKNQTVVAVAGIAKPARFFDSLRNMGITVKR
                     TVALPDHADISAADLPDADAVIITEKDAVKFSDGICTDNVWVLPVCAIIEPDLAAFVL
                     ERLEDVPKAV"
     gene            19564..20145
                     /locus_tag="WGQ68_000687"
     CDS             19564..20145
                     /locus_tag="WGQ68_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HEZ0714534.1"
                     /translation="MKLKTLLLPFATLALCTNAFAAPPSDASLARWLDTQNFDRDIEK
                     NMIEGFNAGFKPYADKALAEMPEAKKDQAAKAFNRYRENVLKDLITPEVKQAVRNTLL
                     KNAREIYTQEEIDGMIAFYGSPAGQSVVAKNPRLIKKSMSEIAVSWTALSGKIARHHL
                     PEFTEELRRIICGGKNPDAGCKQAGQLGKRHQK"
     gene            20214..20396
                     /locus_tag="WGQ68_000688"
     CDS             20214..20396
                     /locus_tag="WGQ68_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEZ0714535.1"
                     /translation="MEKKFLDILVCPVTKGRLEYHQDKQELWSRQAKLAYPIKDGIPY
                     MLENEARALGEEELKV"
     gene            20393..21154
                     /gene="kdsB"
                     /locus_tag="WGQ68_000689"
     CDS             20393..21154
                     /gene="kdsB"
                     /locus_tag="WGQ68_000689"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEZ0714536.1"
                     /translation="MTEFVVLIPARLDSSRLPGKALADIHGKPMVVRVAEQAAKSKAA
                     RVVVATDHPDIQTVCQAHGIEAVMTSNRHESGTTRLAEASAALKLPPHLIVVNVQGDE
                     PLIAPELIDRTAEVLVENNVQMATAAHELHDFDELMNPNAVKVVLDKNRNAIYFSRAP
                     IPYLRDAMRAGKREMPSETAVLRHIGIYAYRAGFLQRYAEMSVSPLETIESLEQLRVL
                     WHGYPIAVETAKEAPAAGVDTQEDLDRVRAVFQTV"
     gene            21177..21494
                     /locus_tag="WGQ68_000690"
                     /pseudo
     CDS             21177..21494
                     /locus_tag="WGQ68_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237659.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
     gene            21599..21796
                     /locus_tag="WGQ68_000691"
     CDS             21599..21796
                     /locus_tag="WGQ68_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714537.1"
                     /translation="MAHGHKARADGLAQQPDKAAEANEEEYLTKALSQNLLSTLDVAL
                     ARFPEDAWFQEIKQDAQKHFA"
     gene            21953..22045
                     /locus_tag="WGQ68_000692"
     tRNA            21953..22045
                     /locus_tag="WGQ68_000692"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21987..21989,aa:Ser,seq:gct)
     gene            22471..23256
                     /locus_tag="WGQ68_000693"
     CDS             22471..23256
                     /locus_tag="WGQ68_000693"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEZ0714538.1"
                     /translation="MSRIRQAFAALDGGKALIPYITVGDPDIRTTLALMHGMVANGAD
                     ILELGVPFSDPMADGPVIQRAAERALANGISLRDVLDVVRKFRETDTQTPVVLMGYLN
                     PVHKMGYREFAQEAAKAGVDGVLTVDSPVETIDPLYRELKDNGVDCIFLIAPTTTEDR
                     IKTIAELAGGFVYYVSLKGVTGAASLDTDEVSRKIEYLHQYIDIPIGVGFGISNAESA
                     RKIGRVADAVIVGSRIVKEIENNAGNEAAAVGALVKELKDAVR"
     gene            23293..24165
                     /locus_tag="WGQ68_000694"
     CDS             23293..24165
                     /locus_tag="WGQ68_000694"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEZ0714539.1"
                     /translation="MSWLDKILPPKIKNRSKDGSSNVPEGLWHKCPSCSATVYSTELQ
                     QNNQVCPKCNHHNPLSARQRLNLLLDEEGREEVAGNVKPIDPLKFKDGKKYPDRLSAA
                     RKLTGEDDALVVMKGKMNGLPVVVAAFEFRFIGGSMGSVVGERFVQGIRRAVADNCPF
                     VCVAASGGARMQEGVNSLMQMTKTSAALHLLTEKRLPFISVLTDPTMGGVSASFAFLG
                     DVVLAEPNALIGFAGPRVIEQTVRETLPEGFQRAEFLLEKGAIDQIVDRRDMKRRISD
                     LITLLCRQDKVSAA"
     gene            complement(24225..24869)
                     /locus_tag="WGQ68_000695"
     CDS             complement(24225..24869)
                     /locus_tag="WGQ68_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cryptic protein cnp1"
                     /protein_id="HEZ0714540.1"
                     /translation="MRRTTLLLLALAAGTSLAAGFSQKDTPINTRYRETPEEAAAREF
                     KEHTAKLPPLPDTHSDGWFDIYVDENYGKQPKILLDSLQIMPAPDGSIRYILNIRSDK
                     GYDNLTAEGIFCARFSIRFGNGKPSSYKVFGYGKPSSYKVFGYGDTVNRRWIQPRNAE
                     WKPIGGTLSRNDALRTVLYQAFCEGGVPADTKGLVQRLKERAGRYAPSIKPHNK"
     gene            24978..25202
                     /locus_tag="WGQ68_000696"
     CDS             24978..25202
                     /locus_tag="WGQ68_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HEZ0714541.1"
                     /translation="MNSETLDVTGLKCPLPILRAKKALAQMQQGDVLTVLATDGGAPG
                     DFEAFCRQTGHVLLDSSEQDGVFTLVVKHK"
     gene            25203..25349
                     /locus_tag="WGQ68_000697"
     CDS             25203..25349
                     /locus_tag="WGQ68_000697"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714542.1"
                     /translation="MPSEMRMSRPTALFLNIVCAACRFKNKPSGVQCRHFGRIAADLF
                     RNGC"
     gene            25565..26599
                     /gene="pyrC"
                     /locus_tag="WGQ68_000698"
     CDS             25565..26599
                     /gene="pyrC"
                     /locus_tag="WGQ68_000698"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEZ0714543.1"
                     /translation="MQTLTIIRPDDMHLHLRDGDALKAVAPYTARQMGRAVIMPNLKP
                     PVVSVADALAYKARIMAALPEGSAFEPLMTLYLTDNATPELVREAKAAGIVAFKLYPA
                     GATTNSDSGVTDLFKLIPVLEEMAKQGILFLVHGEVTDPEIDIFDREAAFIGRVMKPV
                     LAQVPNLKVVFEHITTAEAARLVLEAGDNVAATVTPQHLLLNRNDLLVGGVRPHHFCL
                     PVLKRETHRQALVAAVTGEKAHKFFLGTDSAPHAKSAKENACGCAGMFSAMTAVELYA
                     EVFEKAGALDKLEAFASKNGARFYGIPENAGTITLVKQSQTVPASVPYGDGELVPMRA
                     GGEIGWTVQY"
     gene            complement(26680..27105)
                     /gene="nusB"
                     /locus_tag="WGQ68_000699"
     CDS             complement(26680..27105)
                     /gene="nusB"
                     /locus_tag="WGQ68_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEZ0714544.1"
                     /translation="MKTARRRSRELAVQAVYQSLINRTAAPEIAKNIREMPDFAKADE
                     ELFNKLFFGTQTNAADYIQKIRPLLDRDEKDLNPIERAVLLTACHELSAMPETPYPVI
                     INEAIEVTKTFGGTDGHKFVNGILDKLAAQIRPDEPKRR"
     gene            complement(27183..27659)
                     /locus_tag="WGQ68_000700"
     CDS             complement(27183..27659)
                     /locus_tag="WGQ68_000700"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEZ0714545.1"
                     /translation="MNTIAPNLDGKHLRIGIVQARFTNEIGSEMLKVCCRTLQELGVA
                     DENITVATVPGALEIPIALMNFASSEKFDALIAIGVVIRGETYHFELVSNESGAGVSR
                     VALDYNIPIANAVLTTENDAQAIERIEEKASDAAKVAVECANLVNLLLEEQFEDEE"
     gene            complement(27706..28029)
                     /locus_tag="WGQ68_000701"
     CDS             complement(27706..28029)
                     /locus_tag="WGQ68_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2185 domain-containing protein"
                     /protein_id="HEZ0714546.1"
                     /translation="MNAFAQALSSALDRCIATNTVAKQNQPVGFLYREAPVFENDSGW
                     RFFSGDETDEYTDDPDNFSIVSLADITKTNPETAALLSQPEGSAWELAEDGTFQTVAD
                     WQPKD"
     gene            28196..28915
                     /gene="rnc"
                     /locus_tag="WGQ68_000702"
     CDS             28196..28915
                     /gene="rnc"
                     /locus_tag="WGQ68_000702"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEZ0714547.1"
                     /translation="MKDDVLKQQAHAAIQKKLGYAFRDISLLRQALTHRSCHTKHNER
                     FEFVGDSILNYTVARMLFDAFPKLTEGELSRLRASLVNEGVLAEMAAEMDVGEGLYLG
                     AGELKSGGFRRPSILADAMEAMFAAVSFDADFNTAEKVVRHLFADRVRCADFQNQAKD
                     GKTALQEALQARRFALPKYRIEEQIGHANDSMFVISCDLGELGFVCYAKGTSRKAAEQ
                     EAAKEALKWLEKKLPLKKKKK"
     gene            28962..29885
                     /gene="era"
                     /locus_tag="WGQ68_000703"
     CDS             28962..29885
                     /gene="era"
                     /locus_tag="WGQ68_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEZ0714548.1"
                     /translation="MDIETFLAGERAADGYRCGFVAIVGRPNVGKSTLMNHLIGQKIS
                     ITSKKAQTTRNRVTGIYTDDTAQFVFVDTPGFQTDHRNALNDRLNQNVTEALGGVDAV
                     VFVVEAMRFTDADRVVLKQLPKHTPVVLVVNKIDKDKAKDRYALEAFVAQVRAEFEFA
                     AAEAVSAKHGLRIANLLELIKPYLPESVPMYPEDMVTDKSARFLAMEIVREKLFRYLG
                     EELPYAMNVEVEQFEEEDGLNRIYIAVLVDKESQKAILIGKGGERLKKISTEARLDME
                     KLFDTKVFLKVWVKVKSGWADDIRFLRELGL"
BASE COUNT         7089 a         6977 c         8626 g         7299 t
ORIGIN      
        1 acgacctatt tccttcattt gaaacaggat attgggaatt aagtttttca aaaatcctac
       61 acctgctcct tccacggcag caccttggtc aaaacggcag acggctacaa agccattgcc
      121 catatccgag ccggagaccg cgtcctttcc aaggacgagg caagcggaga aacgggatac
      181 aaacccgtta ccgcccgata cggtaatccg tatcgggaaa ccgtttacat taaagtttca
      241 gacggcatcg gtaaaataca gaccctcgtt tccaataaaa tccacccgtt ttacagtcaa
      301 ggaaaatgga tacaggcagg tcgtctgaaa aaaggcgaca ccctgctttc cgaaagcggc
      361 gcaaaacaga cggttcaaaa cattaccttc aaacagcagc cgctcaaagc ctacaatctg
      421 accgttgccg actggcatac ttacttcgtc aagggtgata gggcggaaac ggaaggggtt
      481 tgggttcata atgattgtcc gtatggagaa aaatatagga cagaagttgg ttcttatact
      541 aatactcatg aaagtggtcg aacttattct ggaaagggca caagacaacg ttcccaaatc
      601 tctggagcaa gagaagctag ggttaataat gaccctcata tagctacaga ttttactcct
      661 gccaaaaata atcgagaggc attcaaagat gaatctagaa gaattgacgc tcacggtggt
      721 tccaaaagta ccaataatta caatcgaatt gaaagcccgg ggaaaaaata ccgaaaacaa
      781 gatggagatt aaaaatgatt gttgacgaaa aatctacttc agatgtactg aaacaatatc
      841 aagaactagg gtttgatttg acaaaaccta tgataataga gttttttata ggaggttctc
      901 aaaagaatct gcaatcaata gaaagccgac tcatttctta tcggcaagat tttcaaatca
      961 gtattgagca aaatgaattc ggagaaatgt ggacttgcta ttgttccgtc aatatcgtcc
     1021 cttcccttga aaacatttta gctattgaaa ctacgttatt tggtattgca caaaaaaatg
     1081 attgtaatta tgaaggtttt ggttcgtatg ggaactaaca aaaagtgtag gttgggttga
     1141 aaaaaccata ttcccattaa agattagggt gtgtctatgg acataaaatt caatactttg
     1201 ggggttatcc tgaatggggt caatcctgaa gaaaaattca taaaaattat tgatgatcag
     1261 gaaaataccg ggggatttct gatactgtta tcttcaaatg acaaattctc atcatttgac
     1321 agttacgatg actgggtcga aaacttagaa atattaaaag aatacctgca agaatctcat
     1381 tggataataa agtgggtcgg atagtcggca aacgtagtcc gtctgaaacg cttttacttt
     1441 tcagacggac tttctatcaa tgggaacaag gcgttttgtc tcaatctggt ctatgattat
     1501 tcgatacagt gatgggaagc taagaaaaat atgattaatc atattgaaaa atatataggc
     1561 actatttctc atggctcgaa aagcgattcg ggaagtcagt acaaattaaa tatcgcagct
     1621 attccctctt cccctaacag ggatttaaaa acttatatta ctttagagtt aagcaaacat
     1681 gatctgaatt ataaaagcag atttgaaata ctttttgtat gttctttaaa atatgatgaa
     1741 aatcagattt tcccattttt aagatggctg gcagaaacca tcattgagaa taaaaaaatc
     1801 cttcttcgag gacaggttat ttatttgcct agaagcattg ttgattcaac aaaaatggat
     1861 gcactgtatg tttccgcccc attttatttc gacgatgatt ttcaggtctg ttatggcgaa
     1921 cactacaata tcgttttccc tttgcttgtc cccttgtata aacaggaagc cgaattggtg
     1981 gaaaaaaagg gttggaatgc ttttgagcag ttcttgctgg ataatgaagt tgacaacctt
     2041 tcggatatga atcggaaacc gtttgtttgg taagggcttt cttaaatttg attaaaggtc
     2101 gtctgaaacc gtgtttaact ttcagacaac ctttccttca tttgaaacag gatattggga
     2161 actaagttct tcaaaaatcc tgtctccgtc ccttatacgt cggtgcttta gtcaaaacgg
     2221 cagacggcta caaagccatc gcacatatcc aaactgggga gtacgtcttt gccaaggacg
     2281 aagcaagcgg aaaaacggga tacaaacccg ttaccgcccg atacggcaat ccgtatcaag
     2341 aaaccgttta cattgaagtt tcagacggca tcggcaacaa ccaaaccctg atttccaacc
     2401 gcatccatcc gttttattcg ggcggcaaat ggattaaagc ggaagatttg aaagcgggaa
     2461 gcaggctgtt atccgaaagt ggcaaaaccc aaaccgtccg caacatcgtt gtcaaaccaa
     2521 caccgctcaa agcctacaat ctgaccgtcg ccgactggca tacctacttc gtcaagggta
     2581 atcaggcgga aacggaaggg gtttgggttc ataatgattg tccgtatgat aaaggcaacc
     2641 aacgatataa agacgcttct tatcatggca aaaatgataa ttctgtgaaa agtagagcac
     2701 caacaaacgg acaagcagct cttgataatt ccgttcaagt taaatcaact tctcctcgaa
     2761 gagttggggt tgataaagcc aataatgaaa tcgttgtatt agacaaaact caaactttta
     2821 ataacggttc tgcggaatat cacgggcatg tcagaagttg gcaagatttg cataccgatc
     2881 agaaaaatgc tttaaaaaaa gcaggattgg attagttaat tcaaaaggaa aaattaaaaa
     2941 atgactgata aaagtaaaac agaaaagttg atttcttctg atgataaaca aagtgttata
     3001 gatggcattc ttgatatggt atttaattcc aaagcatatg aagtaccgtg gatttctgag
     3061 aaattgatgg aattatcgaa aaataaagac ttggatattg ccggattatc gctaacctgt
     3121 ttcggacatc tcgccaggct acattcaaat atcggtgatt acgataaagt tatttcttta
     3181 ctacattcaa agcaagatga tccagagctt caaggtaggg ctgaagatgc gttagaagat
     3241 atttctttat ttttatctga aaatcattag gaaccgtagg tcgggttgaa aacccaacaa
     3301 tcaaaatgcc gtctgaaacc gtgtttaatt ttcagacggc atttccttca tttgaagcag
     3361 gatattgaga actgagttct tcaaaaatcc tacacctgct ctttccacgg cagcaccttg
     3421 gtcaaaacgg cagacggctg aaaagcaaac accgtccgtc gtgttgccgt ttgcggatga
     3481 gtacgggtca accccaatgc cgccgaaacc gtcgaagccg ccttcaacat tgccgccgcc
     3541 aaagccgcaa agttggcaaa aacggtaaaa ctggggagat aaaagccgat ggcaggaaag
     3601 taaatgtgag gatagacagt acggaggcag acctgcttta tccggcaggg caataagaaa
     3661 acaaaaatta gatatggaaa acgattgtga agattaaacc attacaattt tctaacaata
     3721 atcacagatt ttatgtggac aaaatttgtg ttaccgaaca agatgtgaac attttggcat
     3781 ccgaccgaaa ttatgaatta aatattgaaa tatttattga caatataatt cattttcaaa
     3841 taacagatga atcttataaa gtaaaatttt cagaatattt atttgaaaat aaagaaaaaa
     3901 atgattggga tagaaatcct gctataaatt attttttcga gataatagat gacagttata
     3961 tggactggtt gaaagaagaa agttttgatt tttttgaaaa gaaatattat aaggcttata
     4021 ttttcttttt tagcgattct gtaatagaag ttatcagctc gacagaacct gtattttatt
     4081 caaaataaca aattatcaaa caaagctctg attaaaaacc caacaatcaa aataccgtct
     4141 gaaacaatat tcggctttca gacggtattt ttgacacaaa gcaggtaacc aaaggagtgt
     4201 ttgacggaaa aggagaagct aaaataccgg atgtatcggt tgggaagcaa tggataaagg
     4261 taaataatta tgtggaaaat tagtaaagaa aattgtgaag atttaggatt tgcaatagtc
     4321 tgtatgttct atgatgctat taatctttct gaatttaaat tatggttgga tatagttgtc
     4381 agagatactc ctattgatac aattccattg tatatttttg atttgattga ttttgataag
     4441 agtatagggg aaatttatga tgtaattgga gtcgttaatt atggttacat ttcaaatgat
     4501 caaaaaaatg cattaacggg cattgccttc ttaaggggga tagatgtcta tgatccgcct
     4561 atttcaaaag aaaaagcatt aaaagcctta gagaaatatc ctgaaattta tcagaggttt
     4621 cagcatttct ttccttttgt agagcttccg cttctttaaa ggacaagatg ccgtctgaaa
     4681 agttttcaga cggcattttt tatttcttcc agtaggcggg ggtgaagagg atgaagacgg
     4741 tgaagatttc cagcctgccc aagagcatgg cggtaacgca gatccatttc tgcatcacgt
     4801 ccaaaccggc gtaattgccg gcgggcccga cttcgcccag gccggggccg gcgttggtga
     4861 tgcaggcgat gacggcggtg aaggcggtgg taaattccat accgctcgcc atcagcagga
     4921 agctgaagag gacgacggtc ataaagtaga tgaagatgaa ggacataacg gtcagcgcga
     4981 ggcggtcggg tatggctttg ccgctgattt tgacggtgcg gacggctttg gggtgcagca
     5041 gcaccatcat ttcgcgcagg ctgaatttga acaggacgag ggcgcgtatg gttttgatgc
     5101 cgccgccggt cgagccggag ttggcgagga tgttggcgag gaaaaacatc cacagggaaa
     5161 tcaggagcgg ccattgtgcg aagtcggtgt tggccagccc gtttgccagt ccgatggaga
     5221 cgaagttgaa ggcggtgtag cgcagggatt cggtaaaacc ggcgtaatag ccggtgtgcc
     5281 acaggtacag ggcggcggta aggatgctgc cggagagcag cagcagcatc gtccggcatt
     5341 cttcgtcttt ccaataggtt ttgaggctgc ggctgttgag ggcggcgaaa tggctggcaa
     5401 aattgatgcc gccgacaatg gtgaaaacga tgatgaccgc ctcgatgagg ggggagttgt
     5461 aataagctat gctggcatcg tgggtggaaa atccgcccag cgagagggtc gccattgcgt
     5521 gacagacggc atcgaaccag ctcatcccgg caaaatgcag gcaggctgcc gcgaggatgg
     5581 tgatcagggt gtagccgaac cagagttttt tcgccacttg ggaaatgcgc ggcgacattt
     5641 tgctttcttt gtcaatgccg gggatttcgg ctttgaataa ctgcgtgccg cctacgccga
     5701 gcataggcag gatggcgacg gcaaggacga tgatgcccat cccacccagc cagttgagca
     5761 tatgccgcca aaagttgacg gagggggcga gcccgtcgac gtgggggatg acggtcgcgc
     5821 cggtggtggt cagtcccgac atcgattcaa aaaatgcgtc ggtaaagccc atattcggga
     5881 aatacaggta catcggcatc gccgccatag cggcaaacgc cagccacaac atcaggacga
     5941 gggtgaagcc gtcgcgcggg cgcagttcgc gcctgaaccg gagggtggcg agccggacga
     6001 tgtacgagcc ggaaagggta acggtcgcgg tggtggcgaa ggcggtgtac gcgccgtccg
     6061 aaaaggcgta ggagagggcg gcgggtatca gcaggataaa ggaaaacagc atacccagtc
     6121 gggagaggac gtgggcgatg ggcaggattt tgtgcatagt ggggcggtcc gttattttgc
     6181 gaagcttttc cagtctatgc cgccggcggc ttggacttgg gtaatttctt ccgtttcgag
     6241 gttgacggcg gtcagctgtc cgccccacag cgcgccggtg tccagcgaga tgacgttgtc
     6301 ggcattcgtg tagcccagcg aggaccagtg tccgaagatg atggtgcggt cgaggttttg
     6361 ccggtcgggg gctttgaacc acgggcgcag gtaaggcggc atttttttca ctgtggattt
     6421 gtagtcgaaa tccagttcgt ttttaaaggt cagggcgcgc atccgcgtga aggcgttgac
     6481 gatgaagcgc aggcgggcat agcctttcaa accttcgtcc cacgcggccg gtttgttgcc
     6541 gtacattttg gagaagaatt tgacgtattt tttgccgcgc agttcggctt cggcttctct
     6601 agcgagcgat tcggctttgg ttatgcgcca ttgcggcagg atgccggcgt gtaccatcac
     6661 gcggctgccc tcgcgtatca acagcggttg cgcacgcagc cagtcgagca ttttttttcc
     6721 gtcggggtgt ttgagtatgg gttcgattgt gtcgctgcgt ttgggcgcac cttcgccgca
     6781 gccgacagcg agcaggtgca ggtcgtggtt gccgaggacg atttgcacac tgttttcgtg
     6841 ccggatgcag aattgcagcg tttcgaggga tttcgggccg cggttgacga tgtcgcccgt
     6901 cagccagagg gtgtccgtgc cgtggttgaa accgattttg ccgagcagcg cggtcagttc
     6961 gtcgaaacag ccttgtatgt cgccgattgc gtaatgtgcc attgcagatg ttgtgaagtg
     7021 ggaaagtgtt gcggttcgga cggcatggtt ttgaaatatc atgccgtccg aacgtggaat
     7081 tatgcgttca aaacgaggac ggcttcggct tcgacctgca cgcctttggg cagcgaggca
     7141 acgccgacgg cggcgcgggc ggggaagggc tcggcaataa attccgccat cacttcgttg
     7201 aagacggcga aattgcccaa gtcggtcagg taggcgttga gtttgacgat gtcggccagc
     7261 gtgccgcctg ccgcttcggc gatggcttgc aggttttgga acacttggcg cgcttcggcg
     7321 cggaaatcgc cgttgccgac gacggtcatc gtggcgggat cgagggggat ttgaccgctc
     7381 atgtaaacgg tgtcgcctgc tcggacggct tggctgtacg cgccgatggc ggcgggggct
     7441 ttgtcggtgt ggatgatggt tttggacatt tcggattcct caaaaaatag ggcggcagaa
     7501 gccgcagcat tcgggattat cgtacaaaac cgccggcttg tgtagttgcg gtggcagaaa
     7561 acaaaaccgc cgaaggctcg gcggtttgca gaataaggcg catatcagaa tttgacgcgc
     7621 acgccgacgg acagttcgcc ggaacggacg tttttgacag tgttgacttt gccgatgtag
     7681 ttgtagcggt agctggcatc caaatcgaca ttcggggtaa cggcatagct tacgcccgtc
     7741 aatacgccga ggccgatgga ggtttggctg aagctgtcgc tgccgcccaa gtcgacggag
     7801 gcgcggttga ggctcaagcg cgcgccgaga tacggtttga cgggcgattg ggtgtcgaag
     7861 tcgtaaatgg cggacgcgcc gatgctgtaa agtttgaaat cggtggatgg ggctttatag
     7921 tttttgtagc gcgtgtaatc gacggcgaag cggaggtcgt tgatgcggta gcctgcggag
     7981 atgcgcgggc tgaagccttt ggcagaacct aaagagcttg aggcttttgc gtgtgcggca
     8041 tcggcttgga cgtaaaagcc ggatgcgcct tccgccagtg cggcggccgg gatagcgagg
     8101 gcaatcagtg tggcaagtgc ttttttcata ttttggttcc tttatggtca gttagaaaaa
     8161 ttgttaagaa tccgttaaag aatcctgctg tattatactt aaattttctt tttgcatcgt
     8221 aatattttca atacttcaag atacgtagcg gtatccggct gctttgccga cggcaaagcc
     8281 gttaacccgc gcgttgcctt taaatggtgg cggcggcatc acgcggcgga tgggtgaaac
     8341 ttgcaaacgg tttggaaaaa acagcggtat ctgtcggatt gttgcaggtg caggcatacg
     8401 gttttgtgtg cgtctgtgcc ttaagcgtcg gacatttccg gcggcggctg tgccgtctga
     8461 aacgcccggc gggggatgcg gctgcgtttt ccatcgataa gcatattttc cggacgcgtt
     8521 cggggcgggt tttcccgggc ggccgccgat ttgtttgcgc ttatatagtg gattaacaaa
     8581 aatcaggaca aggcgacgaa gccgcagaca gtacagatag tacggaaccg attcacttgg
     8641 tgcttcagca ccttagagaa tcgttctctt tgagctaagg cgaggcaacg ccgtactggt
     8701 ttttgttaat ccactataaa aaacactgca gcaaatcgtt taaaaacaag cgtccttttt
     8761 cggtcgggcg gaatacggca gggtcggttt ccagcaggcc tttttgcctt gccgtttcga
     8821 tttgcgccat gattttggca ctcggtacgc ccgtgcgctc ctgcaacatc gcggcgggta
     8881 cgccgtcggt caggcgcagg gcgttcatca tgaattcgaa cggcaaatct tcggcggcaa
     8941 cggttttgcg ttcgacggct tcactcggtt ggctttgcat taaggcgagg tagtcgttgg
     9001 ggtggcggcg gcggacggtg cgctcgatgc ggtcgggata ggagattttg ccgtgcgcgc
     9061 ccgcgcctat gcccaaataa tcgccgaact gccagtagtt caaattgtgg cggcaccgca
     9121 tggcgggttt cgcaaaagcc gatgtttcgt aatggataaa gccggcgttt tccaatgcgc
     9181 cgtgtaccgc gtcttcgatg tcgagggcgg cttcgtcttg cggcaaacct ttgggcggcg
     9241 tgtgtccgaa cggcgtgttc ggttccatcg tcaggtgata cgcgctgatg tgggtcgcac
     9301 ccgtggcgat ggcggtttgt acgtcatcca atgccgtccg aacggtttgg ttcggcaggg
     9361 cgtacatcaa gtcgatattg attttatcaa ataatttcag ggcagtagct atggcggtta
     9421 aggcttcctt gccgttgtgg acgcgcccca gccttgagag catatcgttg ttgaaactct
     9481 gcacgccaat agaaagccgc gtgatgcctg cgtctttaaa tccttgaaat ttctcgattt
     9541 caaacgtacc cggattggct tccaaggtaa tttccgcttc cggctgcaag cgcaacagcg
     9601 aacgcacgcc gctcaacaaa cggtcaatcg attccgcctg aaacaggctg ggcgtaccgc
     9661 cgccgaaaaa taccgtttcc accggcctgc cccaaatatt gggcaattcg agctgcaagt
     9721 cggtcagcag cgcgtcgata taggcggctt cgggcaatcc gtttttcagg ctgtgggaat
     9781 tgaagtcgca atacgggcat tttttgatgc accacgggat atggatgtaa agcgacaggg
     9841 gcggcagggc ggtgagccgg gtgcggtttg gaaaggaaat ggtgtgcatg gtgcggttcg
     9901 gaaaagtgga caatgccgtc tgaaggcggt tcagacggca caggttcagc cgagcagggt
     9961 aagcagttcg gcttcgctga ggacggaaac gcccaaggca ttggcttttt ccagcttgct
    10021 gcccgcggct tctccagcga cgacgtaatc ggtttttttg gaaacgctgc cggaaacttt
    10081 gccgcctgcg gcttcgatta gggattgggc ttggtcgcgt ttgagggtgg gcagtgtgcc
    10141 agttaacacg aaggttttgc ctgctacggc ttcattgagg ccgtctgaac cttgcgccgc
    10201 ctcgtcttca gacggcattt gcgcgaagaa ggttttcagg ttttcgagta gggcggcgtt
    10261 ttgtggttcg ctgcgccatg cttgccagtc ggcggggagg gctttgtcgt tttgtagacc
    10321 ttctatgttt ttaccagcga gttcccataa ggcttgggct ttgttttcgc taattttgaa
    10381 gtcgggcagg cgggcgatcc agcgttgcgg ttcggcgtgg cgggcgggcg ggatggtaac
    10441 ggcttgggtt tgcggggcaa cgcctgcggc gagcagttcg tctatcatcg cctgctgttc
    10501 ggcttgggcg aagaagtggg caatggaatg cgccactacc gtaccgatgt cgggcaggca
    10561 ggcgaggacg ggttcggggg cgcggcggac gcgttccaat gtgccgaatg cctgcgccag
    10621 cgttttggcg gtgcgttcgc cgacgtggcg gatgccgagc gcgaacagga agcgcgcgag
    10681 ttcgggcgtt ttgccggatt ctatgcctgc gaggatgttt tccgcccact tggtcggttg
    10741 ttttttatgt ttgcccgacg caccgaccga actgccttca gacggcattt gatccgattc
    10801 ggcaacggtt ttgtccgctg tttccttcat tttttgcaag gtcgggatgt cgaggcggta
    10861 gagatcggcg aagtggcgga cgaggtcttg cgcgacaagc tgttcgattt gtttttcacc
    10921 caagccgtcg atgtccatcg ctttgcgcga ggcgaagtgg attaagcctt gcgcgcgttg
    10981 tgcctgacaa agcatgccgc cgctgcatcg ggcgacggct tcgccttctt cgcgttcgat
    11041 ttcgctgcgg cagatggggc agtgggtcgg caggcggtag ggcttgtgga gcggaacgga
    11101 ttgggtttga ttggcggacg gtgtttcggc aaacaaatcg tcctgccgat gcccgatgcc
    11161 gtctgaaacg gcaacggcgg tttcccgcat cgggcggcgt tcaaaaatca cgcgcacaac
    11221 ttcgggaatc acgtctccgg cacggcgtac gacgacggta tcgccgacgc gaacgtcttt
    11281 gcgcgatact tcgtcctgat tgtgcagggt ggcgttggta acagttacgc caccgacgaa
    11341 tacgggctgt aatcgggcaa ccggcgttac cgcgcccgtc ctgccgattt gcacgtcaat
    11401 cgcttcgaca atggtcaggg cttcttcggc agggaatttg tgggcaatcg cccagcgcgg
    11461 cgcgcgggag atgaagccga gttcgtgctg ttgcgccaag ctgttgactt tgacgaccat
    11521 gccgtcgatt tcatagggca gttcggggcg tttctgctgc atttgttcat aaaacgccaa
    11581 tacttcgtcg atatttttga agcagccgaa attgccattg ggcaggctga agccgagttt
    11641 ttggaaatag gcgagttctt ggatgtgttc ttccgcgacg aaaccgcctt gccggcgggc
    11701 gacggagtag gggaaaaagt gcagtttgcg ttgcgcagtg atgcgcgaat cgagttggcg
    11761 taggctgccg gcggcggcgt tgcgcggatt ggcaaagggt ttttgcccgt tttcggcttg
    11821 tcttttattg agggcggcaa aatcggcttt ttgcatcagt acttcgccgc gtacttcgat
    11881 gagttcgggc gtattttcgc cgtgcagccg caaggggatg ttggatacgg ttttgatgtt
    11941 ttgggtaacg tcttcgcccg ttgtgccgtc gccgcgcgtt gccgcctgta ccaatacgcc
    12001 gtcgcggtag agcaggctga tggcgaggcc gtcgaattta ggttcgataa cgtattcggg
    12061 tttgccgccg tccaaaccgt cgcgcacgcg ttggtcgaaa gcgtacattt ctgcatggtc
    12121 gaacacgccg ttttcatctt gcggggaaaa ggcgttggtc agcgacagca tcggcacttc
    12181 gtggcgtact tcggcaaatc ccgccagagg ctcgccgccg acgcgctggg tcgggctgtc
    12241 gggcagtttg agctcgggat ggtttcgttc caacgcttcg agctcgcgga ataatttgtc
    12301 gtattcggca tcgggtacgc tgggcgcgtc gagggtgtag tattcgtagg catagcggtt
    12361 gagcaggtcg gtgaggtggc ggatgtgttg tgcggcaaat tgttttatat cactatcaga
    12421 cggtttaaga agattggtaa agttagtgtt atgttttgag tttggattca tgagagaagg
    12481 ttttcagacg acctttgtct gatacgggat gaaacgggca aaggtcgtct gaaaaatgat
    12541 aggttgaaaa cagctgaatt ttacccgaaa aaaagcggat atgccgtaac gacatatccg
    12601 ctttgattgc attcgatttt aggagaacag gcgcagggcg gtttttcctc cgggctggat
    12661 accgattttg agcatttcct cctgacgtgc caaaacgtag gtgcgtacgt ctttaagcca
    12721 ttgggtagat acttcttcca ttttgtcgtt aaccaaattg aggttcagtt ggccggacag
    12781 tcggactgcc aaatccataa agaggtcgtc aaaagttttt tcgcctgccg gggagtgcgg
    12841 aatatcgagc agcatactga agcctttgta ggactggttg tccaaaaggg cattggtaaa
    12901 cggctcgttg ttgagcgagc agatggagaa catggtcgaa cccgacgtgt cggtatagtg
    12961 gaacgcgccg tcgtcttcca aaacgaaacc cacgcccgtt acggcggaac gcagttctac
    13021 gccgctgatg ctggtcgggg aaaccaaatg gatggcgatg gtctgatcga cgcgcgcgca
    13081 gaatgcgtcc agtgcggaag ccacttcgat aaaggcggca aggtcggtgt gcagcgtctg
    13141 accacccatg ctttgtgcga atgcgtccac ctggcggttg aatgcggaga gttcttcctg
    13201 cgaggcaagt ccgttgcggc tgactgcctg aatacccacg ataaatgcct gatagcggat
    13261 gcccgggatg ggttcggcaa tctggaaatg gtcgtccatg gtgcagccga caatctggta
    13321 gcggcagcgg ttggaaaggc gcggcagtgc gtgcagttct ttggcttcgg tcagcgcgat
    13381 ataggagatg aagtcgaagc gcacgtcaaa ccagggcagc tcgaccttcg acagctcttt
    13441 gagcgtaatc agcggttttg tcggcgtttg cgggacagga atgggttttg ccggcgcgtc
    13501 ggcaggtttc ggtgcggaat gtccggtttg gggttcggaa acggtgtggg cggagttgcc
    13561 gataatgccg ctttcttcca aggcggtttc gatttcggtt ttgaacgggg aggcttttgc
    13621 ctgtttctgc ttggcgatgt agacggcatc ctgttcttgc aggttgcgca tggcgggatc
    13681 ttggggtttt gccgtttttt tgaccgccgg ttggggtttc ggcatcatga ctggcccgcc
    13741 ggacggtttg ccgtcgcgga catggctggt tttgctgttg agcagggcat ctttgtcgga
    13801 gtgtccgaac tggtcgcgca cttttttgcg gtattggttt tcctgataca tattgtaggc
    13861 gacaacggcg aggacggcgg cgaggaacag tacgatgtaa atcatggcaa tcactttaaa
    13921 tttcgggatg caggatacgc aaagtgcggg tactgcggtt aaatcgggct tgcgtttccg
    13981 gcagtctgac ggaacggccg gttataacgt ttgaattata acgaaaattg cagggtctga
    14041 cagcagtgtg tcgaaataag cggaaatttt ccgaaatgcc gtctaaaatc tgtggttttc
    14101 agacggcatt tctgtccacg ggaaaccctt tcccccgtat ccgccgccag tcgaaaaaat
    14161 gaccggggtc ggttttgcgg ccgggcgcga tgtcttggtg ccccgttacc gccgtgatgg
    14221 ggtagcggcg gcagattgcg tccaacaagg cttcgagcga acggtattgc gcttcggtaa
    14281 agggttcgaa atcgcagcct tccagttcga tgccgatgga aaatgcgttg catttttccc
    14341 gtccgcgaaa cgaggatacg ccggcatggt atgccatatt gtcgcaggaa acgaactgta
    14401 ccgtttcgcc gtcgcgtttg attaaaaaat ggctggatac gcgcaaagtg tgtatcaggc
    14461 tgaagaacgg atgtccgtcg gggtcgagcc ggttggcaaa cagcttttcc accgcatccg
    14521 tgccgtattt gaagggcggc agcgaaatgt tgtgcagcac gatcagggaa accgtttccc
    14581 ccgtttccct cgggctgaaa ttgggcgacg gggtatggcg tatgctttga agccagccgt
    14641 tttgccagtg tgcttcggcg tgattgtcca tgatgttctt cctgtccggc gggtaatttg
    14701 ggttatactg tcgcccgaat tttaagacgt attccgaatg ctgggaatcc taccatgttg
    14761 aaaaaaatgt tgaaatggac tgccgttttt ttgaccgtat cggcagccgt tttcgccgcg
    14821 ctgctttttg ttcctaagga taacggcagg gcataccgaa tcaaaattgc caaaaatcag
    14881 ggtatttcgt cggtcggcag gaaacttgcc gaagaccgca tcgtgttcag caggcatgtt
    14941 ttgacggcgg cggcctacgt tttgggtgtg cacaacaggc tgcatacggg gacgtacaga
    15001 ttgccttcgg aagtgtctgc ttgggatatc ttgcagaaaa tgcgcggcgg caggccggat
    15061 tccgttaccg tgcagattat cgaaggttcg cgtttttcgc atatgaggaa agtcatcgac
    15121 gcaacgcccg acatcgaaca cgacaccaaa ggctggagca atgaaaaact gatggcggaa
    15181 gttgcgcccg atgccttcag cggcaatcct gaagggcagt ttttccccga cagctacgaa
    15241 atcgatgcgg gcggcagtga tttgcagatt taccaaaccg cctacaaggc gatgcaacgc
    15301 cgcctgaatg aggcatggga aagcaggcag gacgggctgc cttataaaaa cccttatgaa
    15361 atgctgatta tggcgagcct ggtcgaaaag gaaacagggc atgaagccga ccgcgaccat
    15421 gtcgcttccg tcttcgtcaa ccgcctgaaa atcggtatgc gcctgcaaac cgacccgtcc
    15481 gtgatttacg gcatgggtgc ggcatacaag ggcaaaatcc gtaaagccga cctgcgccgc
    15541 gacacgccgt acaacaccta cacgcgcggc ggtctgccgc caaccccgat tgcgctgccc
    15601 ggcaaggcgg cacttgatgc cgccgcccat ccgtccggcg aaaaatacct gtatttcgtg
    15661 tccaaaatgg acggcacggg cttgagccag ttcagccatg atttgaccga acacaatgcc
    15721 gccgtccgca aatatatttt gaaaaaataa accatgccgt ctgaaaagtt tgtgttttca
    15781 gacggcatac ccttaccgga actgcaagca tgaaaccgca attcatcact ttggacggca
    15841 tagacggagc cggaaaatcc accaaccttg ccgtcatcaa agcatggttt gaacggaggg
    15901 ggctgcccgt gctgttcacg cgcgagccgg gcggaacgcc ggtcggtgag gccttgcgcg
    15961 aaatcctgct caaccctgaa accaaagccg gtttgcgtgc ggaaacactg atgatgttcg
    16021 ccgccagaat gcagcatatt gaagacgtga tattgcctgc actttcagac ggcatccatg
    16081 tcgtgtccga ccgttttacc gatgcaacct tcgcctatca gggcggcggg cggggaatgc
    16141 cgtctgaaga cattgaaatt ttggaacatt gggtgcaggg cggtttgcgc cccgatttga
    16201 ccctgctgtt ggatgtgcca ctcgaagtgt cgatggcgcg tatcgggcag acgcgcgaga
    16261 aagaccgttt cgagcaggag caggcggatt tctttatgcg tgtgcgcagc gtttatctga
    16321 accgtgccgc cgcctgtccc gaacggtacg ccattatcga cagtaaccgc agcttggatg
    16381 aagtcagaaa caacatagaa aaagtgttgg acggacattt cggctgctga tgcggcaaat
    16441 attgaaacaa gcgcatccgc ccgcgccgaa aatcaaacgg cagtgccgca ggtgaaaatg
    16501 gcggtatgcg ccaaactttc ggcatgatag aattacgctc ggttacaagg caggatgcgt
    16561 cggcaatatt aacgaaccgc ccgtaacatg atgacccgaa agcgtttcgg acagtccgat
    16621 tcaaatcttt ttctcgcaac aggattgaca catggaaaac tcattgaaag aagccgccct
    16681 caagttccac gaattacccg ttccgggcaa aatttccgtt accccgacca aatctctggc
    16741 gaccgacaaa gatttggcgt tggcgtactc tccgggcgta gccgctcctt gtatggaaat
    16801 ccatgccgat ccgcaaaatg cctacaaata cactgctaaa ggcaacttgg tcgccgtcat
    16861 ttccaacggt acggccgttt tgggcttggg cgacatcggc gcgctggcgg gcaaacccgt
    16921 gatggaaggt aaaggcgtat tgttcaaaaa attcgccggt gtggatgtgt tcgacatcga
    16981 aatcgatgaa aaagacccgc aaaaactggt ggatatcatc gctgctttag agccgacctt
    17041 cggcggcatc aacctcgaag acatcaaagc acccgagtgt ttctacatcg aacgcgaatt
    17101 acgcaaacgc tgcaaaatcc ccgtattcca cgatgaccag cacggcacgg ccatcattac
    17161 cgccgccgcc gtattgaacg ctctgcgttt taccggccgt aaaatcgaag aagcgacctt
    17221 ggtttgctct ggtgcgggtg cggccgcgat tgcctgcttg aaccaattgc tggatttggg
    17281 cttgaaacgc gaaaacgtaa ccgtttgcga ctccaaaggc gtgatttacc aaacccgcga
    17341 agacaaagat cgcatggacg aatccaaaaa attctacgcc gttgaagaca atggccagcg
    17401 cgtacttgcc gatgccgtca aaggtaaaga catcttcttg ggcctctccg gcgcgaacct
    17461 gctgaccccc gaaatgttga acacgatgaa cgaaaaaccc atcgtgttcg ccatggccaa
    17521 cccgaatccg gaaatcctgc cgccgctggc aaaagaaacc cgtccggacg tggttatcgg
    17581 taccggacgt tcagacttcc cgaaccaagt caacaacgta ttgtgcttcc cgttcatctt
    17641 ccgcggcgcg ttggatgtcg gcgcgaccac catcaacgaa gaaatgaaac gcgcctgcgt
    17701 gtatgctttg gcggatttgg cgatggaaga ggtaaccgaa gaagtggttg ccgcttacgg
    17761 taagaaattt gaattcggcg cggaatacct gattccgact ccgttcgatt cccgcctgct
    17821 gccgcgcgtc gctacggctg ccgccaaagc agcgatggaa agcggcgtgg caacccgtcc
    17881 gattgccgac tttgaagcct acgccgccaa gctgggcgaa tggaagctgt aagccgtttg
    17941 tggttaaaat gccgtctgaa ctgttttcag gcggcatttt gctgtcagat tgataaatga
    18001 aagatactgg aaaatgaaag agatggagac tgtccgttat catatcggcg atatgcccga
    18061 gacttcaaaa caaaccgccc ccgtcatgac gacagggcag tgggtgttga cgatgattgt
    18121 tttcatgatt cctttggtca atttttgttt gggtgttcgg cagaggcaac ccgaaccgcg
    18181 ccaatttctg taaagcgcag ttgcttattt acctgattgg ttcgcttatc ggtttggtct
    18241 tcgcgttgtt tataggtggg tctgtatcgg gtacgcatga ttaatgcccc gggctgattt
    18301 tgcttcgagg atttgtatcg aatatgccga attgtttcaa atttcatacc gttatcgaac
    18361 ggcattggca aaaaccttat ccggttttgt cttttctgct taagccgctc tccgggctgt
    18421 ttgccaaaat tgcggcaaaa cggcggacgg attttttatc gggaaaacgg caaagcgaaa
    18481 agctgcccgt gcctgtggtt gtggtcggca atatccatgc cggcggtaca ggaaaaacgc
    18541 cgattgttgc cgcgctggtg tcgggtttgc aggaaaaggg cgtcaaggtc ggcatcatca
    18601 gccgaggcta cgggcgcaag agcaaggcgg ttcatgtatt gaatgccgag agccgagcgg
    18661 aagatgcggg cgatgagcct ttgctgctgt tccgcaaaac cggtgcgccg acggcggtgg
    18721 gcagcagccg tgcagaggca ggcagggcgt tgctggcggc gcatcccgac atcggactga
    18781 ttgtggcgga cgacggtttg cagcattacg ccctgcggcg agatgtggaa atcgcggtgt
    18841 ttccggcggc ggatacgggg cgcacggatt tggacttgct gcccaacggc agtttgcgcg
    18901 aacctttgtc gcggctggat tcggtggata cggtcgtcgt cagcggcggc aaggcggatg
    18961 cgctgtttag gccgtctgaa aatatgtttc acagccgtat cgaagtggga cgaatttacc
    19021 gtttgaacaa tccgtccgaa atactggaca caggccgtct gaaaaatcaa accgtcgtcg
    19081 ccgtggcagg tattgccaag ccggcgcgtt ttttcgattc gttgcggaat atgggcatta
    19141 ccgtgaagcg aaccgtcgcg ctgcccgacc acgccgacat ttcggcggca gatttgcccg
    19201 atgcggacgc ggtcattatt acggagaaag atgcggtcaa attttcagac ggcatttgca
    19261 ccgataatgt ttgggtgttg cccgtttgtg cgataatcga acctgatttg gcggcgtttg
    19321 tgttggagcg gttggaagat gtaccgaagg ccgtctgaaa gcacggtttg ggcggagtga
    19381 ttacggattt gaataagaac gcctcgcgcc gtcattcccg cgcaggcggg aatctaagtc
    19441 tcgaattttc aggaatgcct aggaggcttc agaaatccca aatctccgga tttccacttg
    19501 gacaggaatg agaaaaccgg tcgtattttt tatctgcatt aatcattcat taaaggattg
    19561 aatattaaac tgaaaacctt gttattgccc ttcgccacgc tggcattgtg caccaatgct
    19621 tttgccgctc cgcccagcga cgcgtcgttg gcgcgttggc tggatacgca gaattttgac
    19681 cgggatatag aaaaaaatat gattgagggc tttaatgccg gatttaaacc gtatgcggac
    19741 aaagcccttg ccgaaatgcc ggaagcgaaa aaagatcagg cggcaaaagc ctttaaccgc
    19801 tatcgtgaga atgttttgaa agatttgatt acgcccgaag tgaaacaggc tgtccgcaat
    19861 actttattga agaatgcccg tgagatatac acgcaagaag aaattgacgg catgattgcc
    19921 ttttacggtt cgcccgccgg tcagtccgtc gttgccaaaa atccgcgctt aatcaagaaa
    19981 tcgatgagtg aaatagcggt atcttggact gcattgtcag ggaaaatcgc gcgacatcat
    20041 ctgcccgagt ttacggaaga gttgcgacgc atcatctgcg gcggtaaaaa tcccgatgcg
    20101 ggctgcaagc aggccggaca gcttgggaaa aggcatcaga aataaatgat agccgtctga
    20161 aatattgcag agggcatccg attgattaaa ccatcaaacc cgaaagcaac cttatggaaa
    20221 aaaaattctt agacatcctc gtctgccccg ttaccaaagg caggctggaa tatcatcagg
    20281 acaaacagga attgtggagc cgtcaggcga agctggcgta tccgattaaa gacggcatcc
    20341 cctatatgct ggaaaacgaa gcgcgagcgt tgggcgaaga ggagctcaaa gtatgaccga
    20401 attcgtcgta ttgattccgg cgcggctgga ttcgtcgcgc ctgcccggaa aagccttggc
    20461 ggacatccac ggcaaaccga tggtcgtgcg cgttgccgaa caggcggcaa aaagtaaagc
    20521 cgcgcgcgtc gtcgtcgcca ccgaccatcc cgatattcag acggtctgtc aggcgcacgg
    20581 catcgaagcg gttatgactt caaaccggca cgaaagcggc acgacgcgcc ttgccgaagc
    20641 ctctgccgcg ctgaagctgc cgccgcattt gattgttgtg aacgtacagg gtgacgagcc
    20701 gctgattgcc cccgaactca tcgaccgcac cgccgaagta ctcgtcgaaa acaacgtcca
    20761 aatggcgacc gccgcccacg aattgcacga tttcgacgaa ttgatgaatc ccaatgccgt
    20821 caaagtcgtc ctcgacaaaa accgcaacgc catctacttc agccgcgccc cgattcccta
    20881 tctgcgcgat gcaatgcgtg ccggaaaacg cgaaatgccg tctgaaaccg ccgtcctgcg
    20941 acatatcggc atctacgctt accgcgccgg cttcctgcaa cgctatgccg aaatgagcgt
    21001 ttcgccgctg gaaaccatcg aatcgctgga acagctgcgc gtcctgtggc acggttatcc
    21061 cattgccgtc gaaaccgcca aagaagcccc cgccgccggt gtggatacgc aagaggactt
    21121 ggacagggtt cgcgccgtat ttcagaccgt ataaaacagg ttcaaaggga aaagatatgc
    21181 agcaacatat tgaaaagtgg caacacttga gccgggaaga acagaaaatc cttgctgaag
    21241 tatggggtct cgtgcaaaac gacgatcagg aggttcacta tgaaatgctc aaattgaacg
    21301 cacccgatga agccagcggc gaattttggt tcagaatggc agaaacactc agcaccctgc
    21361 cgcccaaccg ttccctcggc cttagaatga acggcggcag gctggcgacc gccgtatcca
    21421 tcctttccgt catgattgaa gacaatcccg acataccgca gctttgggcg caaaaaatta
    21481 ccgcgctcaa ttatagtgga ttaaatttaa accagtacgg cgttgcctcg ccttgtcgta
    21541 ctatctgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactatattt
    21601 ggcacacgga cacaaagccc gtgccgacgg tttggcacaa cagcccgaca aagcggcaga
    21661 agccaacgag gaggaatacc tgaccaaagc cctgtcgcaa aacctgctgt caacattgga
    21721 tgtcgcgctt gcacgttttc ctgaagacgc gtggtttcag gaaatcaaac aggatgcaca
    21781 aaagcatttt gcttgaggat gtggcagtca ggaatatttc cattcaggaa gaaaagaagt
    21841 gcctgattgg gtataatcag ggtaaatctt attttatttc aaaagattaa tatttgcttt
    21901 ctgtttttcc ttgacggtat cggaaaagtt gattatagtt acagcttcct taggagtaat
    21961 ggctgagagg ctgaaggcac ttccctgcta aggaagcatg tggggtcaac ctgcatcgag
    22021 ggttcgaatc cctcttactc cgccagataa aaaatagacg ctgtgtttta cagcgtctat
    22081 ttttttatgc aattttatag cgggttggtg caaaaccagt atggtattgc cctgtcttga
    22141 ttctgaattt tgttatagtg gattaacaaa aaccagtacg gcgttgcctc gccttagctc
    22201 aaagagaacg attctctaag gtgctggagc accaagtgaa tcggctccgt actatttgta
    22261 ctgtctgcgg cttcgtcgcc ttgtcctgat ttttgttaat ccactataat ccgagatgct
    22321 tgccgtttat ttccgcctcg ttcaaacggc ggctctgatt tgcgcggttt ctgtttgccg
    22381 tattcgccta tccgtaccgc aaatgttata ctgggaaaaa tttactgatt gtgttttacg
    22441 gcatatttgc cgataggatg gaagagacaa atgagcagaa tccggcaggc ttttgccgct
    22501 ttggatggcg gaaaggcatt gattccctat attacggtgg gcgaccccga tattcggaca
    22561 actttggcat tgatgcatgg catggttgca aacggtgcgg atattttaga gttgggcgtg
    22621 ccgttttccg atccgatggc ggacgggccg gttattcagc gtgcggcgga gcgggcgttg
    22681 gcaaacggga tttcgctgcg cgatgtcttg gatgtcgtca gaaaattccg tgaaaccgac
    22741 acgcaaacgc cggttgtttt gatgggatat ttgaaccctg tacataagat gggttatcgg
    22801 gagtttgctc aggaagccgc aaaggcgggt gtggacggcg tgttgacggt ggattcccct
    22861 gtcgaaacca tcgatccgct ctatcgcgag ctgaaggata acggggtcga ctgtattttc
    22921 ctgattgcgc cgacgacgac ggaagaccgt attaaaacca ttgccgagct ggcaggcgga
    22981 tttgtctatt atgtttcgct caagggcgta acgggcgcgg caagtttgga tacggatgag
    23041 gtttcgcgta aaatagagta tttgcatcag tatatcgata ttcccatcgg tgtcggtttc
    23101 ggcatcagca atgcggaaag cgcgcgcaaa atcggccggg ttgccgacgc ggtcattgtc
    23161 ggcagccgga ttgtgaaaga gattgaaaac aatgcgggca acgaggctgc cgccgtcggt
    23221 gctttggtca aagagttgaa ggatgccgtg cgctgattct gtcacgcatt ctaaatattt
    23281 taggagttgt ccatgagctg gttagataaa atcctgccac ccaaaatcaa aaaccgcagc
    23341 aaagacggtt cttccaatgt tcccgagggt ttgtggcaca aatgtccgtc ttgttcggca
    23401 accgtttatt cgaccgagtt gcagcagaac aatcaggttt gtccgaaatg caaccaccac
    23461 aatccgttgt cggcacgaca acgcctgaat ctgcttttgg atgaggaagg cagggaggaa
    23521 gttgcgggta atgtcaaacc gatagatcct ttgaagttta aagacggcaa aaaatatccg
    23581 gatcgtttga gtgcggcacg caagctgacc ggggaagatg atgctttggt ggtgatgaaa
    23641 ggcaagatga acggtctgcc cgtcgtcgtt gccgcgtttg aattccgctt tatcggcggt
    23701 tcgatgggtt cggttgtggg cgaacgtttc gtacaaggta tccgtcgggc ggttgccgac
    23761 aattgtccgt tcgtctgtgt ggcggcctcc ggcggtgcgc gtatgcagga gggtgtgaac
    23821 tcattgatgc agatgacgaa aaccagtgcc gcgctgcatt tgctgacgga aaaacgcctg
    23881 ccatttatat cggtgttgac cgatccgact atgggcggcg tatccgccag cttcgcattt
    23941 ttgggcgatg tcgtgcttgc cgaaccgaac gcgctgatcg gttttgccgg tccgcgcgtg
    24001 attgagcaga cggtgcgcga aacgctgccg gaaggcttcc aacgcgccga gttcctgctg
    24061 gaaaaaggcg caatcgacca gattgtcgac cgccgcgata tgaagcggcg catcagtgat
    24121 ttgattacgc tgttgtgccg tcaggacaaa gtttccgccg cttgatggct gatgaatcga
    24181 ataccgtctg aaaccgatgt ttcagacggt atttttgtgt ctggttattt gttgtgcggc
    24241 tttatcgacg gggcatagcg tccggcacgt tctttcaggc gttgtaccaa acctttcgtg
    24301 tcggcgggta caccgccctc gcagaatgcc tgatacagga cggtgcgcag tgcgtcgttg
    24361 cggcttaatg taccgcctat cggtttccat tcggcgtttc ggggctgtat ccagcggcgg
    24421 ttgaccgtgt cgccgtagcc gaacacttta taggaggaag gtttgccgta gccgaacact
    24481 ttataggagg aaggtttgcc gttgccgaac ctgatggaga agcgggcgca gaatatgcct
    24541 tcggcagtca ggttgtcgta gcctttgtcg gagcggatat tgagaatgta gcggatgctg
    24601 ccgtcgggcg cgggcataat ttgcaggctg tcgagcagga ttttcggctg tttgccgtaa
    24661 ttttcatcca cataaatgtc gaaccagccg tccgagtgcg tatcgggcag cggcggcagt
    24721 ttggcggtat gttctttaaa ttcgcgggcg gcggcttctt cgggcgtttc gcggtagcgg
    24781 gtgttgatcg gcgtgtcttt ttggctgaag ccggcagcaa gggacgtgcc agcggcgagt
    24841 gcgagcagca ggagggtggt gcggcgcata agtttctcca aattgaaaac ggcgttattt
    24901 tatgggttgg caaagggggc tgcaagcaac tggggtataa tctccccccg attcccattt
    24961 tttaaacggt acaaacgatg aacagcgaaa ctttagacgt aaccgggctg aaatgccctt
    25021 tgcctatttt gcgtgccaaa aaggctttgg cgcaaatgca gcagggcgac gtgttgaccg
    25081 ttctggcaac cgacggcggc gcaccggggg attttgaggc tttttgccgc caaaccggtc
    25141 atgtgctgtt ggattcttcc gaacaggacg gcgtgttcac gctggtcgtc aagcacaaat
    25201 aaatgccgtc tgaaatgcgg atgtcccgcc cgacggcgtt gtttttgaat atcgtatgtg
    25261 ccgcgtgccg tttcaaaaac aaaccgtccg gcgtgcagtg tcggcatttc gggcgtatcg
    25321 ccgccgattt gttccggaat ggctgttaac cgccttgccg tcggcaaaga agcaaaaccc
    25381 aagcacaatc aaaatctaaa ggctgtgttt gaagattccg ttgatgcaac ccaatcagtc
    25441 ggacaaaatg cctttcatcc aatggaaccg gtttccgacc ggacggaata accggctttc
    25501 ccctggcaaa cggatggaat cgaccgggta ttcaaacgca gccaaaacct aaaaaggaac
    25561 aaccatgcaa accctgacca ttatccgccc cgacgatatg cacctgcacc tgcgcgacgg
    25621 cgacgcgctc aaagccgttg ccccttatac cgcccgccaa atggggcgcg ccgtcattat
    25681 gcccaacctc aaaccgcctg tcgtcagtgt agccgacgcg cttgcctaca aagcgcgcat
    25741 tatggcggcg ttgcccgaag gcagcgcgtt tgagccgttg atgacgcttt atttgactga
    25801 taacgccacg cccgaacttg tacgcgaagc caaagccgcc ggcatcgtcg ccttcaaact
    25861 ctaccctgcc ggcgcgacca ccaattccga ttccggcgta accgacctgt tcaagctcat
    25921 ccccgtgttg gaagaaatgg cgaaacaggg cattttgttc ctcgttcacg gcgaagtaac
    25981 cgaccccgaa atcgacatct tcgaccgcga agccgccttt atcgggcgcg tgatgaaacc
    26041 cgttttggcg caagtgccga atcttaaagt cgtgttcgaa cacatcacca ccgccgaagc
    26101 cgcccgcctg gttttggaag caggcgacaa cgtagccgcc accgttaccc cgcaacacct
    26161 cctgctcaac cgcaacgacc tcttggtcgg cggcgtgcgc ccccatcatt tctgcctgcc
    26221 cgtactcaaa cgcgaaaccc accgtcaggc attggttgcc gccgttaccg gcgagaaggc
    26281 gcacaaattc ttcctcggca ccgactccgc gccgcacgcc aagtccgcca aagaaaacgc
    26341 ctgcggctgc gccggtatgt tcagcgcgat gaccgccgtc gagctttacg ccgaagtgtt
    26401 tgaaaaagca ggcgcgttgg acaaactcga agcctttgcc tcgaaaaatg gcgcaaggtt
    26461 ttacggcatt ccggaaaacg ccggcacgat caccctcgtc aaacaaagcc aaaccgtccc
    26521 cgcaagtgtt ccctacggcg acggcgaact tgtcccgatg cgcgcgggcg gcgaaatcgg
    26581 ctggacggta cagtattgat tgaacggtca agcaaaatgc cgtctgaaag gtttttcaga
    26641 cggcatttat gtattttcca attcggattt tccggagtgt caacggcgtt tgggctcgtc
    26701 ggggcggatt tgggcagcga gtttgtcgag gatgccgttg acgaatttgt gcccgtccgt
    26761 gccgccgaag gttttggtaa cttcgatggc ttcgttgata atgacggggt agggcgtttc
    26821 gggcatggcg gacagctcgt ggcaggcggt cagcaggacg gcgcgttcga tgggattgag
    26881 gtctttttcg tcgcggtcga gcagggggcg gattttttgg atgtagtccg ctgcgttggt
    26941 ttgcgtaccg aagaaaagtt tgttgaacaa ttcttcgtcc gctttggcaa agtcgggcat
    27001 ttcgcggatg tttttagcaa tttcgggcgc ggcggtgcgg ttgataaggg attggtaaac
    27061 ggcttgtacg gcaagctcgc gggaacggcg gcgggctgtt ttcatgattt ttccttgaaa
    27121 cggttgggcg gcacggtatg ccgtctgaaa cggaaagggc gtattggtgt acgccctgtt
    27181 tgttattctt cgtcttcaaa ctgttcttcg agcagcaggt tgacgaggtt ggcgcattcg
    27241 acggcgactt tggcggcatc cgaggctttt tcttcaatcc gttcgattgc ctgtgcgtcg
    27301 ttttcggtgg tcaggacggc attggcaatc gggatgttgt agtcgagtgc gacgcggctg
    27361 acccctgctc cggattcgtt ggaaaccagc tcgaaatggt aggtttcgcc acggataacg
    27421 acgccgatgg caatcagtgc gtcaaatttt tcagaagagg caaagttcat cagtgcgatg
    27481 gggatttcaa gtgcgccggg tacggtggcg acggtaatgt tttcgtctgc cacgcccaat
    27541 tcttggaggg tgcggcagca gactttgagc atttcgctgc cgatttcgtt ggtgaaacgt
    27601 gcctgtacga tgccgatgcg gaggtgtttg ccgtcgaggt tgggggcgat ggtgttcatt
    27661 gggtgtcctt tggtattcgg aggtttcgga atgccgtctg aagggtcagt ccttaggttg
    27721 ccagtcggcg acggtttgga atgtgccgtc ttctgccaat tcccacgcgc tgccttcagg
    27781 ttgggagagc agtgcggcgg tttcagggtt ggttttggtg atatcggcga ggctgacgat
    27841 gctgaagttg tccgggtcgt cggtgtattc gtcggtctcg tcgccgctga agaaacgcca
    27901 gccgctgtcg ttttcaaaaa cgggggcttc gcggtagagg aagccgacgg gctggttttg
    27961 tttggcgacg gtgttggtgg cgatgcagcg gtcgagtgcc gaggaaagtg cttgtgcaaa
    28021 tgcgttcatt gcgggaatac gttgggggaa aacttgcgga ttttaccacg attcttgcgt
    28081 tgtcggcaga cggcggcggt ttggtggtac aatgtgcgcc gtttgcagcc ttaaggtgtt
    28141 tctgtatttt tggagtatgg aaacgcattc gggctgtttt ttgcggaaga cggtaatgaa
    28201 agacgatgtt ttgaaacagc aggcacacgc ggcgatacag aagaaactgg gctatgcgtt
    28261 ccgcgatatt tcgcttttgc ggcaggcttt gacgcacagg agctgccata cgaaacataa
    28321 tgaacggttt gagtttgtcg gcgattcgat tttgaattat acggtggcac ggatgctgtt
    28381 tgacgcgttt ccgaagttga ccgagggcga gttgtcgcgg ttgcgggcga gtctggtcaa
    28441 tgagggcgtg ctggcggaaa tggcggcgga aatggatgtc ggagaaggct tgtatttggg
    28501 ggcgggcgag ttgaagagcg gcggctttag acggccttcg atactggcgg acgcgatgga
    28561 ggcgatgttt gccgccgtca gcttcgatgc cgatttcaac acggcggaaa aggtggtacg
    28621 ccatttgttt gccgatcgag tccggtgtgc cgatttccaa aatcaggcaa aagacggcaa
    28681 aactgctttg caggaggcgt tgcaggcgcg ccgtttcgct ttgccgaaat accgtatcga
    28741 agagcaaatc ggccatgcca acgacagtat gtttgtcatt tcctgcgatt tgggcgaact
    28801 gggtttcgtg tgctatgcca aagggacgag ccgcaaggcg gcggaacagg aggcggcgaa
    28861 agaggctttg aaatggctgg aaaagaagct gccgctgaag aagaaaaaga aatgaggcgg
    28921 cgcgtgaata tgccgtctga aatatggata tgaaagcgaa tatggatatt gaaaccttcc
    28981 ttgcagggga acgtgccgcc gacggatacc gttgcggctt cgtggcgatt gtcggtcgtc
    29041 cgaacgtggg caaatcaacg ctgatgaacc atctcatcgg tcagaaaatc agcatcacca
    29101 gcaaaaaggc gcagacgacg cgcaaccgcg taacggggat ttataccgac gataccgcgc
    29161 agtttgtgtt tgtcgatacg cccggttttc aaaccgacca ccgcaacgcg ctcaacgacc
    29221 gtttgaatca aaacgttacc gaggcactcg gcggtgtgga tgccgtggtt ttcgtcgtgg
    29281 aggcgatgcg ctttaccgat gccgaccgag tcgtgttgaa acaactgccc aagcacacgc
    29341 cggtcgtttt agtggtcaac aaaattgaca aggacaaggc gaaagaccgt tacgcgttgg
    29401 aggcgtttgt tgcccaggtg cgcgccgaat ttgaatttgc ggcagcggag gcagtcagcg
    29461 cgaaacacgg attgcggatt gccaacctgt tggagctgat taaaccgtat ctgcccgaaa
    29521 gcgtgccgat gtatcccgaa gacatggtta cggacaaatc ggcgcgtttt ttagcgatgg
    29581 aaatcgtgcg tgaaaaattg ttccgctatt tgggcgagga attgccttat gcgatgaacg
    29641 tcgaagtgga gcagtttgaa gaggaagacg gtttaaaccg catctatatc gccgttttgg
    29701 tcgataagga aagccaaaag gcgattttaa tcggcaaagg cggggagcgt ttgaagaaaa
    29761 tttccaccga agcgcggttg gatatggaaa aactgtttga taccaaagtg tttttgaaag
    29821 tgtgggtcaa agtcaaatcc ggttgggcgg acgacatccg cttcctgcgc gagctgggtt
    29881 tgtagttttt cttgctgaac tttacgcaaa tgccgtccga acaggtttca gacggcattt
    29941 tgtttcaatc gggaatatct ttgttaaaaa cgggttgata ttatctgtgc a
//
LOCUS       DAWFHY010000018        27088 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.018, whole genome shotgun sequence.
ACCESSION   DAWFHY010000018 DAWFHY010000000
VERSION     DAWFHY010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 27088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.018"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            13..300
                     /locus_tag="WGQ68_000704"
     CDS             13..300
                     /locus_tag="WGQ68_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0714549.1"
                     /translation="MALYKYQPSSKYFGQSMAVIAQSEFVEFAKINKSENVIDCFSFF
                     WNRRIKHDIWLISFSDNSEMVIKESLKDGHKIYKFEFCEIVDNCNFDDVFV"
     gene            284..679
                     /locus_tag="WGQ68_000705"
     CDS             284..679
                     /locus_tag="WGQ68_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714550.1"
                     /translation="MMYSFEANANAVKISETVSVDTGQGAKIHKFVPKNSKTYSSDLI
                     KTVDLTHIPTGAKARINAKITASISRAGVLAGGGKLARLGAKFSTRAVPYVGTALLAH
                     DVYETFKEDIQARGYQYDPETDKFAKVSG"
     gene            complement(1179..1254)
                     /locus_tag="WGQ68_000706"
     tRNA            complement(1179..1254)
                     /locus_tag="WGQ68_000706"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1219..1221),aa:Asn,seq:gtt)
     gene            complement(1347..1706)
                     /locus_tag="WGQ68_000707"
     CDS             complement(1347..1706)
                     /locus_tag="WGQ68_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEZ0714551.1"
                     /translation="MQQLLISILEDLNNTSTDIIASAVISTDGLPMATMLPSHLNSDR
                     VGAISATLLALGSRSVQELACGELEQVMIKGKSGYILLSQAGKDAVLVLVAKETGRLG
                     LILLDAKRAARHIAEAI"
     gene            complement(1729..2373)
                     /locus_tag="WGQ68_000708"
     CDS             complement(1729..2373)
                     /locus_tag="WGQ68_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEZ0714552.1"
                     /translation="MESTLSLQANLYPRLTPAGAFYAVSSDAPSAGKTLLHSLLKADA
                     DEMVSSEKLLTWADTADIDTALNLLYRLQKLEFLYGDENGHSDGINLSDEQLPLLMEQ
                     LSGSGKALLVDRNGLYLANANFHHEAAEELGLLAAEVAQMEKKYRLLIKNNLYINNNA
                     WGVCDPSGQSELTFFPLYIGSTKFILVIGGIPDLGKEAFVTLVRILYRRYSNRV"
     gene            complement(2375..2911)
                     /locus_tag="WGQ68_000709"
     CDS             complement(2375..2911)
                     /locus_tag="WGQ68_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HEZ0714553.1"
                     /translation="MRENKIIFTGPVGVGKTTAIAAISDEALVQTDASASDMTLDRKR
                     NTTVAMDYGAISLDEDAKVHLYGTPGQERFNFMWEILSQGSMGLVLLLDNARTNPLKD
                     LEFFLHSFRGLLEKAPVVVGITKMDIRSQPGIDVYHKYLAKHNLNVPVFEIDARKEDD
                     VKQLVSAMLFSIDPGLEV"
     gene            complement(2923..4020)
                     /locus_tag="WGQ68_000710"
     CDS             complement(2923..4020)
                     /locus_tag="WGQ68_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEZ0714554.1"
                     /translation="MEVQLPKIKTVRVMLAGMTAQQESVFKMAFKMHNTTRYETVSPS
                     DGSAVPDLVLADTDAEGGFELWKELAERYKDIPVAVCSEKVPDSEVPYLPKPIRFETL
                     FPMLRKLLQGENVYGKSFIAPADRSAKNNGNVQRTVTIRQFNPNKGLLGALRFAEKNR
                     QDIAILHGNKPVLIVFPSIQRVLLTESVQKLEELCKDENLQVSCKTVPDNPQWREKAK
                     VGIMSCMWQFSIWTAQGRLIYPISPDTPFTLKSWPNLTRLANVPGSIRLSAFLTKASV
                     NLNVLYKVMPLNLNDILNYLAATYTTGFLSVDLKTVSQQAYSDMADKINIGADSASDS
                     EMMKKAEKITTPSQSQSRGLLQRLMKKLLGS"
     gene            complement(4023..5459)
                     /locus_tag="WGQ68_000711"
     CDS             complement(4023..5459)
                     /locus_tag="WGQ68_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEZ0714555.1"
                     /translation="MENSLIIGLALAVLLMLLVMRAKQGKKTPKRKSQGVGNTQGQTP
                     GSNDSDWVDQIGQSVSDGTQPDWSWNESAETASAAVSAQEVDPLTEYQVYKQFGYQGK
                     AAESLAAYLDGIPDGEAKPENLIRELLDINLEVGDVDVLADNLQKYGKLIPSELLAKY
                     IEQALQRDSNHLRIRVLAEEGLGWGTQEIEKRAEGGSATAASASPPPDAGGKAYEAEE
                     IKRIPIVRGKKDVSGISQEEIGAIAGLVRADQGAKILKDKVSYETASKQYDRAIQTSE
                     KPANLIIDALKLDYQYADIDRFAGHLWKLYQTLGNYGRQVKERMLGWGYSLGYHEVFD
                     DLEKGPNDRQIKDIGMGHGYLPKNIQKFKSQHRDLVLQDSSLINTGSSPADDAVKEVE
                     SLLMYGQIEAAMDVLEQAVLKYPDESQLYITLIDIYERTEDWDRLGQFLRVLRERADR
                     LPEEVVMLMSRLLQRMNQNIKKIKRYGK"
     gene            complement(5679..6461)
                     /gene="bioC"
                     /locus_tag="WGQ68_000712"
     CDS             complement(5679..6461)
                     /gene="bioC"
                     /locus_tag="WGQ68_000712"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HEZ0714556.1"
                     /translation="MESLTAINKSRIRQAFQKALNDYDRHALIQQKMTINLMAHLQDY
                     LPDMPLENVLELGCGSGMLSALLQKQISANYWLFNDLCDVQPQLAEKLPQSFDFYCGD
                     AENFPFQRQFDLIASASAVQWFHQPDAFIAHCKTGLKANGLLAVATFGKDNLKEVRQI
                     TNIGLNYPTLSQWQTWLAKDFELLWCEDFKVILDFDTPLDVLKHLKYTGVTATNQKNW
                     TRKNLNGFIGDYLSAFSMPSGKVHLTYHPLFFIARYSAAGRQ"
     gene            complement(6449..7096)
                     /locus_tag="WGQ68_000713"
     CDS             complement(6449..7096)
                     /locus_tag="WGQ68_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF452 family protein"
                     /protein_id="HEZ0714557.1"
                     /translation="MKTKFYNHQGEHLILYFAGWGTPPDAVNHLILPENHDLLICYDY
                     QDLNLDFDFSAYRHIRLVAWSMGVWAAERALQGIRLKSATAVNGTGLPCDDNFGIPFA
                     VFKGTLENLTGNTRLKFERRMCGDKASFEDYQQFSARPFDEIHQELTALFAMIGQDRR
                     TDFIRWTNALVGSGDKIFMSANQHRYWTARCTVREIDGGHYLFSRFTHWSALWNH"
     gene            complement(7106..8248)
                     /gene="bioF"
                     /locus_tag="WGQ68_000714"
     CDS             complement(7106..8248)
                     /gene="bioF"
                     /locus_tag="WGQ68_000714"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HEZ0714558.1"
                     /translation="MKVFKQQLEQLGAQNQYRSIPDLIHQGRYITRENCKMLNMSSND
                     YLGLASDENLRRSFLQQYGGNFPSFTSSSSRLLTGNFPIYTDLEELVAQRFQRESALL
                     FNSGYHANLGILPALTTTKSLILADKFVHASMIDGIRLSRCAFFRYRHNDYEHLKNLL
                     EKNVGKFDRTFIVTESVFSMDGDVADLKQLVQLKKQFPNTYLYVDEAHAIGVYGQNGL
                     GIAERDNLIAEIDLLVGTFGKALASVGAYAVCNQVLKECLINQMRPLIFSTALPPFNV
                     AWTYFIFERLPQFSKERSHLEQLSAFLRREVAHRTQIMPSQTCIVPYILGGNEATLAK
                     AEYLQRQGYYCLPIRPPTVPKGTSRIRLSLTADMTMDEVRQFAACL"
     gene            complement(8270..8407)
                     /locus_tag="WGQ68_000715"
     CDS             complement(8270..8407)
                     /locus_tag="WGQ68_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009346942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase"
                     /protein_id="HEZ0714559.1"
                     /translation="MATETNVAEISALDYEGRGVAKVGGKTVFIKRALLDCLMLGWFR
                     L"
     gene            8471..9547
                     /locus_tag="WGQ68_000716"
     CDS             8471..9547
                     /locus_tag="WGQ68_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEZ0714560.1"
                     /translation="MICRKPIGHTPYFDMNKTICRTAALLISGFSYANTVIPDVSPVA
                     QGQHVFINIPQQRLFLYTDGKLTKVYPVAVGRAMTQTNLGEHKIGAKAYNPVWYIPKS
                     IQKERGDGVKTIAAGPDNPLGPVFVRLGDPKLGLGIHGTNAPASVPGVRSHGCVRMKS
                     PDALEFAKTIASGSPASVIYQMAGLNEDADRNLWLAAFRDPYGKNNLDIASLKKSIAQ
                     WAKTQGKTIAPEKVDAVLKDRTGSAVCLTCGKNGKMKMPLKSLAWIQGSSSYSQAEVI
                     EQTEETNSAEISETRTPEVPDVHTPEAQPHLNTQSDGTPTAYTEPAADSSPQVETPDQ
                     AASEPVDVLFSIDVIRQGNLRLGN"
     gene            complement(9754..11220)
                     /locus_tag="WGQ68_000717"
     CDS             complement(9754..11220)
                     /locus_tag="WGQ68_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEZ0714561.1"
                     /translation="MMKLGFKPIPLAIAAVLCALVLALPVPDGVKPQAWTLLAMFVGV
                     IAAIIGKAMPLGALSIIAVGLVAVTGVTADKPGAAMSDALSAFANPLIWLIAIAVMIS
                     RGLLKTGLGMRIGYLFIAVFGRKTLGIGYSLALSELLLAPVTPSNTARGGGIIHPIMQ
                     SIAGSYGSNPAKGTEGKMGKYLALVNYHSNPISSAMFITATAPNPLIVNLIAENLGSS
                     FRLSWGAWAWAMAVPGVIAFFVMPLILYFLYPPEIKETPNAVQFAKDRLREMGKMSAD
                     EIIMAVIFGILLLLWADVPALITGNHAFSINATATAFIGLSLLLLSGVLTWDDVLKEK
                     SAWDTIIWFGALIMMAAFLNKLGLIKWFSGVLAESVGGLGVSGTAAGVILVLAYMYAH
                     YMFASTTAHITAMFGAFLAAAVSLNAPAMPTALMMAAASNIMMTLTHYATGTSPVIFG
                     SGYTTMGEWWKAGFIMSVVNFLIFFVIGSIWWKVLGYW"
     gene            complement(11418..12341)
                     /gene="speB"
                     /locus_tag="WGQ68_000718"
     CDS             complement(11418..12341)
                     /gene="speB"
                     /locus_tag="WGQ68_000718"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEZ0714562.1"
                     /translation="MQYSTLAGQTDNSLVSNNFGFLRLPLNFMPYESHADWVITGVPY
                     DMAVSGRSGARFGPEAIRRASVNLAWEHRRFPWTFDVRERLNIIDCGDLVFSFGDSRD
                     FVEKMEAHAGKLLSSGKRCLSLGGDHFITLPLLRAHARYFGKLALIHFDAHTDTYDNG
                     SEYDHGTMFYTAPKEGLIDPSRSVQIGIRTEHSKKLPFTVLTAPQVNEDSVEETVRKI
                     KETVGNMPVYLTFDIDCLDPSFAPGTGTPVCGGLSSDRALKILRGLTDLDIVGMDVVE
                     VAPSYDQSDITALAGATIALEMLYLQGAKKD"
     gene            complement(12440..14332)
                     /gene="speA"
                     /locus_tag="WGQ68_000719"
     CDS             complement(12440..14332)
                     /gene="speA"
                     /locus_tag="WGQ68_000719"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HEZ0714563.1"
                     /translation="MPILTIREVCNINHWGIGYYDVDDSGEIIVRPNPSQHNQTVSLQ
                     KLTEAVQQKHQARLPVLFCFPQILEHRLRDINRAFQTAREECGYKGGYCLVYPIKVNQ
                     HRRVIESLMSSGQPHGLEAGSKAELMAVLAHAGTRQTLIVCNGYKDREYIRFALMGEK
                     LGHQVYLVIEKLSEIQMVLEEAEKLGIKPRLGVRARLASQGSGKWQSSGGEKSKFGLS
                     ASQVLQLVDILKQKNRLDCLQLLHFHLGSQLGNIRDVATGVHESARFYVELHKLGVNI
                     RCFDVGGGLGVDYEGNRTQSDCSVNYSLNEYASTVVWGISQACLEHGLPHPTIITESG
                     RGITAHHAVLVANVIGVERYKPRRLDAPSPEAPRVLHSMWETWTDISASREKRSLRSW
                     IHEGQFDLADVHNQYNVGLLSLAQRAWAEQLYLNICHEVGELFNEKHRSHRTIIDELQ
                     ERFADKLYVNFSLFQSLPDAWGIDQLFPVCPITGLNEPIARRAVLLDITCDSDGTIDH
                     YIDGDGIAGTMPMPDYPEEEPPLLGFFMVGAYQEILGNMHNLFGDTATADVVVGEDGQ
                     FTVIDYDEGNTVADMLEYVYQDPKELMKRYREQIEHSDLPASQAMSFLKELEAGLNGY
                     TYLEDE"
     gene            complement(14499..14783)
                     /locus_tag="WGQ68_000720"
     CDS             complement(14499..14783)
                     /locus_tag="WGQ68_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0714564.1"
                     /translation="MKKLLLAAVVSLSAATAFASDSAERQIYSDPNFEQNRVKAVKML
                     EQRGYQVHDVDADDHWGKPVLEVEAYKDGREYDIVLSYPDLKIIKEQLDR"
     gene            complement(14934..16742)
                     /gene="aspS"
                     /locus_tag="WGQ68_000721"
     CDS             complement(14934..16742)
                     /gene="aspS"
                     /locus_tag="WGQ68_000721"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEZ0714565.1"
                     /translation="MRTNYCGLISEQYLDQTVTVKGWVHRRRDHGGVIFIDLRDREGI
                     VQVVIDPDTPEAFAAADSSRNEYVLSITGRVRNRPEGTTNDKMISGKIEILAKEIEVL
                     NAAATPPFQIDDENISENVRLTNRVIDLRRPAMQRNLRLRYQVAMGVRRYLDAQGFID
                     IETPMLTRSTPEGARDYLVPSRVHPGEFFALPQSPQLFKQLLMVAGFDRYYQITKCFR
                     DEDLRADRQPEFTQIDLETSFLNEDEIMDITEGMAKQVFKDALNVDLGDFPRMPYAEA
                     MFYYGSDKPDMRINLKFTELTDLMKTEEFKVFRGAADMKGGRVVALRVPNGAKFSRKE
                     IDEYTKFVGIYGAKGLAYIKVNDVGNLSNGEDSGLQSPIVKFLSENALKEIIERTGAQ
                     NGDIIFFGADKTKVVNEAIGALRIKVGLEHGKDNGYFTDEWKPLWVVDFPMFEYDEEA
                     DRYVAVHHPFTAPKEGHEDLMVSDPANCLARAYDMVLNGWEIGGGSIRIHRADVQEKV
                     FAALKISPEEQQEKFGFLLDNLKFGAPPHGGLAFGLDRLVTLMTGAESIRDVIAFPKT
                     QRAQCLLTNAPNSVDDKQLRELSLRLRQKAAETKEA"
     gene            complement(16800..17501)
                     /locus_tag="WGQ68_000722"
     CDS             complement(16800..17501)
                     /locus_tag="WGQ68_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HEZ0714566.1"
                     /translation="MTEPAAEGGKAAKALKKYLITGILVWLPIAVTVWVVSYIVSASD
                     QLVNLLPKQWRPQYVLGFNIPGLGVIVAIAVLFVTGLFAANVLGRQILAAWDSLLGRI
                     PVVKSIYSSVKKVSESLLSDSSRSFKTPVLVPFPQPGIWTIAFVSGQVSNAVKAALPK
                     DGDYLSVYVPTTPNPTGGYYIMVKKSDVRELDMSVDEALKYVISLGMVIPDDLPVKTL
                     AGPMPSEKADLPEQQ"
     gene            complement(17644..18792)
                     /locus_tag="WGQ68_000723"
     CDS             complement(17644..18792)
                     /locus_tag="WGQ68_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HEZ0714567.1"
                     /translation="MPTMGAEMNTRNMRYILLTGLLPMASAFGETALQCAALTDNVTR
                     LACYDRIFAAQLPSSAGQEGQESKAVLNLTETVRSSLDKGEAVIVVEKGGDALPADSA
                     GETADIYTPLSLMYDLDKNDLRGLLGVREHNPMYLMPLWYNNSPNYAPGSPTRGTTVQ
                     EKFGQQKRAETKLQVSFKSKIAEDLFKTRADLWFGYTQRSDWQIYNQGRKSAPFRNTD
                     YKPEIFLTQPVKADLPFGGRLRMLGAGFVHQSNGQSRPESRSWNRIYAMAGMEWGKLT
                     VIPRVWVRAFDQSGDKNDNPDIADYMGYGDVKLQYRLNDRQNVYSVLRYNPKTGYGAI
                     EAAYTFPIKGKLKGVVRGFHGYGESLIDYNHKQNGIGIGLMFNDLDGI"
     gene            complement(18953..19216)
                     /gene="rpsT"
                     /locus_tag="WGQ68_000724"
     CDS             complement(18953..19216)
                     /gene="rpsT"
                     /locus_tag="WGQ68_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEZ0714568.1"
                     /translation="MANSAQARKRARQSVKQRAHNASLRTAFRTAVKKVLKAVEAGDK
                     AAAQAVYQESVKVIDRIADKGVFHKNKAARHKSRLSAKVKALA"
     gene            19519..20661
                     /locus_tag="WGQ68_000725"
     CDS             19519..20661
                     /locus_tag="WGQ68_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0714569.1"
                     /translation="MKKSVLAVLAALSLAACGGSEKNAVQPQADAASAANAEAAATDT
                     LNIYNWSNYVDESTVEDFKKANNLKLTYDLYENNETLEAKMLTGKSGYDLVVPGIAFL
                     PRQIEAGAYQKVNKDLIPNYKNIDPELLKMLETADPGNQYAVPYFSGVNTIAITAKGK
                     ELLGGKLPENGWDLLFKPEYTHKLKSCGIALWDTPSEMFPILLNYLGKDPKGSNPEDL
                     KAAAEVLKSIRPDVKRFSPSIIDELARGDICLAAGNGGDLNLAKARSEEVKNNVGIEV
                     LTPKGMGFWIESWLIPADAKNVANAHKYINYTLDPEIAAKNGIAVTFAPASKPAREKM
                     PAELVNTRSIFPNEQDMKDGFVMPQMSTDAKKLSVSLWQKIKVGTN"
     gene            complement(21933..24680)
                     /gene="tbpA"
                     /locus_tag="WGQ68_000726"
     CDS             complement(21933..24680)
                     /gene="tbpA"
                     /locus_tag="WGQ68_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein TbpA"
                     /protein_id="HEZ0714570.1"
                     /translation="MQQQHLFRLNILCLSLMTALPVYAENVQAGQAQEKQLDTIQVKA
                     KKQKTRRDNEVTGLGKLVKTADTLSKEQVLDIRDLTRYDPGIAVVEQGRGASSGYSIR
                     GMDKNRVSLTVDGLAQIQSYTAQAALGGTRTAGSSGAINEIEYENVKAVEISKGSNSV
                     EQGSGALAGSVAFQTKTADDVIGEGRQWGIQSKTAYSGKNRGLTQSIALAGRIGGAEA
                     LLIHTGRRAGEIRAHEDAGRGVQSFNRLVPVEDDNQYAHFVVEEECESGSYEKCKSKP
                     KKDVVGKDERQTVSTRDYTGPNRFLADPLSYESRSWLFRPGFRFENKRHYIGGILEHT
                     QQTFDTRDMTVPAFLTKAVFDANSKQAGSLPGNGKYAGNHKYGGLFTNGENGALVGAE
                     YGTGVFYDETHTKSRYGLEYVYTNADKDTWADYARLSYDRQGIGLDNHFQQTHCSADG
                     SDKYCRPSADKPFSYYKSDRVIYGESHRLLQAAFKKSFDTAKIRHNLSVNLGFDRFGS
                     NLRHQDYYFQNAKRAYLLKTPPKNGNQKISPNGSTSTPYWVSIGRGNVVTEQICRLGN
                     NTYTDCTPRSINGKSYYAAVRDNVRLGRWADVGAGLRYDYRSTHSDDGSVSTGTHRTL
                     SWNAGIVLKPADWLDLTYRTSTGFRLPSFAEMYGWRAGVQSKAVKIDPEKSFNKEAGI
                     VFKGDFGNLEASWFNNAYRDLIVRGYEAQIKDGKETAKGDPAYLNAQSARITGINVLG
                     KIDWNGVWDKLPEGWYSTFAYNRVRVRDIKKRADRTDIQSHLFDAIQPSRYVVGLGYD
                     QPEGKWGVNGMLTYSKAKEITELLGSRALLNGNSRNTKATARRTRPWYIVDVSGYYTI
                     KKHFTLRAGVYNLLNYRYVTWENVRQTAGGAVNQHKNVGVYNRYAAPGRNYTFSLEMK
                     F"
     gene            complement(24767..26917)
                     /locus_tag="WGQ68_000727"
     CDS             complement(24767..26917)
                     /locus_tag="WGQ68_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0714571.1"
                     /translation="MNNPLVNQAAMVLPVFLLSACLGGGGSFDLDSVDTEAPRPAPKY
                     QDVSSEKPQAQKDQGGYGFAMRLKRRNWYPSAKENEVKLNESDWEATGLPTDPKELPK
                     RQKSVIEKVETDDDSNIYSSPYLTPSNHQSGSAGNGVNQPKNKATNHENFQYVYSGWF
                     YKHAKQHLDLTNKIVQQGDDGYIFYHGKEPSRQLPASGKITYKGVWHFATDVKKSQNF
                     REIIQPSKSQGDRYSGFSGDDGEEYSNKNETTLQSGHEGYGFTSNLEVDFDNKKLTGK
                     LIRNNANTSNNQATTQYYSLEAQVTGNRFNGKATATDKPGNGETKQHPFVSDSSSLSG
                     GFFGPKGEELGFRFLSDDQKVAVVGSAKTKDKLENGAAASGSTDAAASNGAAGTLSEN
                     GKLTTVLDAVELKSGDKEVKNLDNFSNAAQLVVDGIMIPLLPKDSESGGNQADKSKNG
                     KNGKNGGTDFTYKTTYTPESDKKDTQAQTGAGGAQAASGTESVNGGQAGTKTYEVEVC
                     CSNLNYLKYGMLMRKNSKSAMQAGGNSSQADAKTEQVEQSMFLQGERTDEKEIPTDQN
                     VVYRGSWYGHIANGTSWSGNASDKEGGNRAEFTVNFAEKKITGTLTAENRQAATFTID
                     GKIEGNGFSGTAKTAELGFDLDQKNTTRTPKAYITDAKVQGGFYGPKAEELGGWFAYS
                     DDKQTKNATDASGNGNSASSATVVFGAKRQQPVQ"
BASE COUNT         6775 a         6887 c         6394 g         7032 t
ORIGIN      
        1 acgcaggcgc agttggcact ttataaatat cagccgtcta gcaagtattt tgggcaatcg
       61 atggcggtta tagcgcaatc tgaatttgtt gaatttgcga agattaataa gtctgaaaat
      121 gttattgatt gtttctcttt tttctggaat agaagaatta aacatgatat ttggctaatc
      181 tcattttctg ataattcaga aatggtaatt aaagaatccc tgaaagatgg tcataaaata
      241 tacaaatttg aattttgcga aattgtcgat aattgcaatt ttgatgatgt attcgtttga
      301 agcgaatgca aatgcagtaa aaatatctga aactgtttca gttgataccg gacaaggtgc
      361 gaaaattcat aagtttgtac ctaaaaatag taaaacttat tcatctgatt taataaaaac
      421 ggtagattta acacacatcc ctacgggcgc aaaagcccga atcaacgcca aaataaccgc
      481 cagcatttcc cgcgccggcg tattggcggg gggcggcaaa cttgcccgct taggcgcgaa
      541 attcagcaca agggcggttc cctatgtcgg aacagccctt ttagcccatg acgtatacga
      601 aactttcaaa gaagacatac aggcacgagg ctaccaatac gaccccgaaa ccgacaaatt
      661 tgcaaaggtc tcaggctaag tgcgcctgtt gccgcctaaa aggcggtccg gatgcctgat
      721 tatcgggtat ccggggagga ttaaggggat atttgggtag aattaggagg tgttatggag
      781 cgaaaacagc cgaaaacctg tgttggggtt tcggctgtcg ggagggaaag gaattttgca
      841 aaggtctcaa ttagtatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
      901 agtacaaata gtacggaacc gattcactcg gtgcttcagc accttagaga atcgttctct
      961 ttgagctaag gcgagccaat gccgtactgg tttttgttaa tccactataa aatcgagttt
     1021 atcctagctg tccaggacag cccctatttt ttcataacca tcacgaaagg aattttgcaa
     1081 agattttatt ccatctcaaa acaatcatct caaaaatgcg tttctgaccg ccggtaaaac
     1141 aaaaccctct aagaaaatac ttagagggtt ttgaaatttg gctccccgac ctgggctcga
     1201 accagggacc tgcggattaa cagtccgtcg ctctaccgac tgagctatcg gggaataagc
     1261 gcgaataata aaacagaaat accgaacgtg tcaatataaa ttgacaatcg attgcacttt
     1321 atctgcaagc ccgcaatctt tatatgttat atggcttccg caatatgcct tgccgcacgt
     1381 ttggcatcca ataggattaa accaagtctg ccggtttctt ttgccaccag caccaacacg
     1441 gcatctttac ccgcctgact taaaaggata tagcctgatt ttcctttaat catcacttgt
     1501 tccaattccc cgcaggcgag ttcctgcacc gagcgactcc ccaaagcaag caaagtggca
     1561 gaaatcgccc ctaccctgtc cgaattcaaa tgtgaaggaa gcattgtcgc catcggcaat
     1621 ccgtcggttg agataacggc agacgcgata atatccgtag atgtattgtt taaatcttca
     1681 aggattgaaa tcaataattg ctgcataacc cctcctctct cccaagtttt acacgcggtt
     1741 gctgtaacgg cggtataaaa tccttaccaa agtaacaaat gcctctttgc ccaaatcggg
     1801 aatgccgccg ataaccaaaa taaatttggt tgaaccgata tacaatggga aaaatgtcaa
     1861 ttcgctctga ccggaaggat cgcaaacgcc ccaagcgtta ttgttgatat acaggttgtt
     1921 cttaatcagc agccggtatt tcttttccat ctgtgcgact tctgccgcca acaaccccaa
     1981 ctcttccgcc gcctcatgat ggaaattggc gttggcaaga tacagaccgt tccgatcgac
     2041 caataacgcc ttaccgctgc cggacaattg ttccatcagc aacggcaatt gctcatccga
     2101 caaattgatg ccgtctgaat gaccgttttc atcgccatag aggaattcga gtttttgcaa
     2161 acggtacaac aggttcaaag cggtatcgat gtcggcggtg tccgcccagg taagcagctt
     2221 ctcactgctg accatttcgt ccgcatccgc tttcaacagg ctgtgcaaca aagttttacc
     2281 ggcactgggg gcatcgctgg atacggcata aaatgcaccg gcaggagtca ggcggggata
     2341 taaatttgct tgtagtgaaa gtgttgattc catattaaac ctccagtccc ggatcaatag
     2401 aaaataacat tgcgctaacc aattgtttta cgtcatcttc cttacgggca tcaatttcaa
     2461 aaaccggaac attaagatta tgttttgcaa gatatttgtg atacacgtcg ataccgggct
     2521 gagagcgtat atccatcttg gtaataccga caacgacggg tgccttctcc agcagccctc
     2581 gaaacgaatg taaaaagaat tccaaatctt tcaacggatt ggttcgggca ttatctaaaa
     2641 gcaagaccaa acccatactg ccttggctta agatttccca cataaagttg aaccgttcct
     2701 gaccgggcgt accatataaa tggactttgg catcctcatc caagctgatg gccccgtagt
     2761 ccatcgccac tgtcgtattc cttttcctat ccaaagtcat atcggatgcg gaagcatcgg
     2821 tctgaacgag tgcttcgtcc gaaatagccg caatggcagt ggttttccct acgccgacag
     2881 gtcctgtgaa aataatttta ttttctctca tctctccgcc tcttagctgc ccaacagttt
     2941 tttcatcagc ctttgcagaa ggccgcgcga ctgggattgg gatggtgttg tgattttttc
     3001 cgcttttttc atcatttcac tatcagaggc agaatcggct ccgatattta ttttatccgc
     3061 catatcggag tatgcctgtt gtgaaaccgt ttttaaatct accgacaaaa acccggttgt
     3121 ataggttgcc gcaagataat tcagaatatc attgaggttt aaaggcatca ctttatacaa
     3181 cacgttaagg ttgacggatg ccttggtcag aaatgccgac aagcgtatcg accccggcac
     3241 atttgccaac cgggtcaggt ttggccaaga tttcaacgta aacggagtat cgggagaaat
     3301 cggataaatc aacctgccct gcgccgtcca aatggaaaac tgccacatac aggacataat
     3361 gcctacttta gccttttcgc gccattgcgg gttatcggga acagtcttgc agctgacctg
     3421 caaattttcg tctttgcaca attcttcgag tttttgcaca ctttctgtca gcaaaacccg
     3481 ttgtatcgag gggaaaacaa taaggaccgg cttatttcca tgcaagatag cgatgtcctg
     3541 cctgttcttt tccgcaaacc gcaacgcccc caataatcct ttattcgggt taaactggcg
     3601 tatcgtaacc gtacgctgca cattcccgtt atttttcgcc gaccggtctg cgggtgcaat
     3661 aaacgatttc ccataaacat tctcgccctg caacaatttg cggagcatag gaaacaatgt
     3721 ttcaaaccga atcggtttgg gcaggtaggg aacttcagaa tcgggaactt tctccgaaca
     3781 gacggcgacg ggtatatcct tataacgctc ggcaagctct ttccaaagtt caaaaccgcc
     3841 ctcggcatcg gtatccgcca aaaccaaatc gggcacggca ctgccgtctg aaggggatac
     3901 tgtttcataa cgggtggtat tgtgcatttt gaatgccatt ttgaaaacgg attcctgctg
     3961 cgccgtcatc cccgccaaca ttacgcgtac tgttttaatt ttcggcagtt gaacttccat
     4021 ttttattttc cgtaccgttt tattttttta atattttgat tcatacgctg cagcagccgg
     4081 ctcatcagca taacgacctc ttcaggaagc ctgtccgcac gttccctcaa tacccttaaa
     4141 aactgcccca acctatccca atcttcagta cgttcataaa tatcgatcaa cgtaatataa
     4201 agctgggact cgtcgggata tttcaatacc gcctgctcca acacatccat tgccgcttca
     4261 atctgaccat acatcagcaa cgactctact tccttaaccg catcgtctgc cggagacgaa
     4321 ccggtgttga tcaacgaaga atcttgaagc accaaatccc gatgttgcga tttgaatttc
     4381 tgtatatttt tcggcagata cccgtgcccc ataccgatgt ctttgatttg ccggtcgttc
     4441 ggcccttttt ccaaatcatc gaaaacttca tggtaaccca ggctgtaccc ccaccccagc
     4501 atccgctctt taacctgcct gccgtagttg cccaacgttt ggtaaagttt ccacaaatgt
     4561 ccggcaaaac ggtctatgtc cgcgtattgg taatcgagtt tcaacgcatc gataatcagg
     4621 tttgcaggtt tttcggaagt ttggatggca cggtcgtatt gtttcgatgc cgtttcatag
     4681 ctgactttgt ctttaaggat tttcgcacct tgatcggcac ggaccaaacc ggcaatcgca
     4741 ccgatttcct cttgactgat tccggacacg tcttttttgc cccgcacaat cgggatgcgc
     4801 ttgatttctt cggcttcata agccttaccg ccggcatccg gcgggggcga tgcggaagct
     4861 gccgtcgcag aaccgccttc cgcacgtttt tcaatctcct gagtacccca tcccaaacct
     4921 tcttccgcca agacgcggat acgcaaatgg tttgaatcgc gctgtaatgc ctgttcgata
     4981 tattttgcca aaagttcgga aggaatcagt ttgccgtatt tttgcagatt gtctgccaaa
     5041 acatcgacat cccccacttc gagattgata tcgagcagct cgcggataag gttttcaggt
     5101 ttcgcttcac catccggaat gccgtccaga taggcagcca aagattcggc agccttgccc
     5161 tgataaccga attgcttata aacctgatac tccgtaagcg gatcgacttc ttgcgcggat
     5221 acggcggcgg atgcggtctc ggcactttcg ttccaagacc agtcgggttg cgtgccgtct
     5281 gaaaccgatt gtccaatctg atcgacccag tccgaatcat tgcttccggg tgtctgcccc
     5341 tgcgtattgc cgacaccttg ggatttgcgc ttcggtgttt tcttaccctg cttcgcacgc
     5401 ataaccaaaa gcatcagcaa taccgccagt gccaaaccga taattaatga gttttccaaa
     5461 ggatactccc gataccgtaa cgagcggata gctgaccgcc tgcaacggcc attgtccgct
     5521 tttattatta acgatttact gaaatagact tgtaagtttt aaatcatacc ataatttaac
     5581 gtttaacaat atgccttctg cacaagcctc gccatattac cttttaccca catcagtatc
     5641 aatacccgat ataaaaataa ctttgcccat aagctgcctt attgcctgcc cgccgcagag
     5701 tagcgcgcga taaaaaataa cggatgataa gtcaggtgca ctttgcccga cggcatactg
     5761 aacgccgaca agtaatcgcc aataaatcca ttgagatttt ttcttgtcca atttttttgg
     5821 ttcgtggctg ttacgcctgt atatttaagg tgtttgagta catctaacgg cgtatcaaag
     5881 tctagtatta ccttaaaatc ctcacaccat aaaagctcaa aatctttggc taaccaagtc
     5941 tgccattggg ataaagtcgg gtaatttaag cctatatttg taatttggcg gacttctttt
     6001 aaattgtctt tgccaaaggt tgcaaccgcc aataatccgt ttgctttcaa gcctgttttg
     6061 caatgagcga taaaagcgtc gggttgatga aaccattgca cggcagatgc gcttgcgatt
     6121 aagtcaaatt gtcgttgaaa aggaaagttt tccgcatcgc cgcaataaaa atcaaaggat
     6181 tgcggcagtt tttcagccag ttggggctgc acatcgcaca aatcattaaa taaccaataa
     6241 ttcgctgaaa tctgtttttg cagcaaggca ctcaacattc ctgagccaca gcccaattcc
     6301 aacacgtttt ccaatggcat atccggcaaa taatcttgca aatgcgccat tagattaatc
     6361 gtcatttttt gttggattaa ggcgtgccgg tcataatcgt ttaatgcttt ttggaaagcc
     6421 tgccgaatgc gcgatttatt tatggcagtc agtgattcca tagtgccgac caatgggtga
     6481 atcttgaaaa caggtaatgt ccgccgtcaa tttcccgaac ggtgcaacgc gctgtccaat
     6541 atcggtgctg attggcagac ataaaaattt tatcgcccga tccgaccaag gcatttgtcc
     6601 agcggataaa atctgtacgc ctatcttgcc cgatcatcgc aaaaagtgcg gtaagttctt
     6661 gatgaatttc gtcaaacgga cgggcggaaa attgttggta atcttcaaaa gatgctttat
     6721 cgccacacat tctgcgttca aattttaaac gagtatttcc cgtgaggttc tccaatgtgc
     6781 ctttaaaaac ggcgaaaggg ataccgaaat tatcatcgca aggcaaacct gtgccattca
     6841 ctgccgttgc ggattttaat cttattcctt gcaatgccct ctctgccgcc caaacgccca
     6901 ttgaccacgc caccaaacgg atgtgccgat aggcggaaaa atcaaaatcc aaatttaaat
     6961 cttgataatc atagcaaatc aataaatcgt gattttccgg caaaatcaaa tgatttacag
     7021 catcgggcgg cgttccccaa cctgcaaaat acaggattaa atgttcgcct tgatgattgt
     7081 aaaattttgt tttcatatca tatccttaca ggcacgccgc aaactgccgc acttcatcca
     7141 ttgtcatatc tgccgtcaaa gacaatcgta ttctggatgt accttttggt actgtcggtg
     7201 gtctgatggg caggcaataa taaccctgcc tttgcaggta ttccgctttg gcaagggtgg
     7261 cttcattccc gcctaaaata taggggacga tacaggtttg gctcggcatt atttgcgtcc
     7321 gatgcgccac ttcccgccgt aaaaatgcac ttaactgctc aagatggctt ctttcttttg
     7381 agaattgcgg caatcgttca aaaataaaat aagtccaagc cacattaaac ggcggcaatg
     7441 cggttgaaaa aatcaatggg cgcatttgat taatcaaaca ttctttcaat acttggttgc
     7501 agacggcata cgcccccacc gaggctaagg ctttaccgaa agtgccaacc aataaatcaa
     7561 tctcggcaat caaattatcc cgttcggcaa tccccaatcc gttttgccca taaacaccga
     7621 ttgcgtgagc ttcatccaca taaagataag tattgggaaa ctgttttttt aattggacaa
     7681 gctgtttcaa atccgccaca tcgccgtcca tactgaaaac agattcggta acgataaaag
     7741 tgcggtcaaa ttttccgacg tttttttcaa gcagattttt caaatgttca taatcattat
     7801 gacgataacg gaaaaacgca caccggctca aacggatgcc gtcaatcata ctggcgtgaa
     7861 caaatttatc tgccaaaatc aaacttttcg tcgtcgtcaa agcaggcaaa ataccgagat
     7921 tggcgtgata gccgctgttg aacaataacg cgctttcccg ttggaaacgt tgtgcgacaa
     7981 gctcttccaa atcggtataa ataggaaagt tgcccgttaa taaacgcgat gaagaactgg
     8041 taaaagaggg aaaattaccg ccgtattgct gcaaaaaaga ccggcgcaag ttttcatctg
     8101 atgccaaacc caaataatca ttagacgaca tattcagcat tttgcagttt tcccgcgtaa
     8161 tataccgccc ttgatgaatc aaatccggaa tcgaacgata ttggttttgc gcgccgagtt
     8221 gttcaagctg ttgtttaaaa accttcatta tgatgtaaat attcctgagt taaagcctga
     8281 accaacccag catcaaacaa tcaagtaatg cccttttaat aaaaaccgtt ttgccgccga
     8341 cctttgccac acccctgcct tcataatcca aggcggaaat ttccgcgaca tttgtttccg
     8401 ttgccattac ccaatccgtc cgcactgtac cgaagcgcat attttcgcac aaattggtac
     8461 ggtttcaggt atgatttgcc gaaaacctat cggacacaca ccatattttg atatgaataa
     8521 aacaatctgc cgcactgctg cactattgat aagcggtttt tcatacgcaa acaccgtcat
     8581 tcccgatgtc tcccccgtcg cacaaggtca acatgtcttc attaacatcc ctcagcaacg
     8641 cctgttcctc tacaccgacg gcaaactgac caaggtttat cccgttgcag taggtcgggc
     8701 gatgacgcaa accaatctag gcgaacataa aatcggtgcg aaagcctata accctgtttg
     8761 gtacatcccc aaatccatac agaaagaacg cggcgacggc gtaaaaacca tagccgccgg
     8821 cccggacaac cctttggggc cggtttttgt ccgcttaggc gaccccaaac tcggtttggg
     8881 catacacggg accaatgcgc cggccagcgt ccccggggtt cgaagtcacg gctgcgtccg
     8941 catgaaatcg cccgacgcgc tcgagtttgc caaaaccatc gccagcggct cgcccgcctc
     9001 cgtcatctat caaatggcgg gtctcaatga agatgcggat cgcaacctgt ggcttgccgc
     9061 cttccgcgac ccttacggta agaacaacct tgacatcgcc tctctgaaaa aaagtattgc
     9121 gcaatgggca aaaacacagg gtaaaaccat cgcgcccgag aaagtcgatg ctgtactcaa
     9181 agaccgcacc ggatcggccg tctgcctgac ctgcggcaaa aacggcaaga tgaagatgcc
     9241 gctcaaatcg ctggcgtgga tacagggttc ttcctcatac agccaagctg aagtcattga
     9301 acaaactgag gaaaccaact ctgccgaaat atccgagaca cgcacgcccg aagtgcctga
     9361 tgtacacacg cccgaggcac aaccgcattt aaatacccaa tccgacggca cgccgactgc
     9421 ctataccgaa ccggctgccg attcatcgcc gcaagtagaa acacctgatc aggctgcttc
     9481 cgagccggtt gatgtattat tttcaataga tgtgatacgg cagggaaatt tgcgtttagg
     9541 taattgaata accttctgat tatttaatgc ttatgtttat cagaagttga taggcggttt
     9601 ggtttttgtt tggcttttcc tacaccgccg cctgccgctt tctgcaacat tcaagcgcac
     9661 aaatatgcca tctgaaggct ttagacggca tatttcacga taatagtcgc ccaatcaaat
     9721 aaacacagat tggaaattta ttttattttt cccttaccaa taccccagaa ctttccacca
     9781 aatgctgccg ataacgaaaa aaatcagaaa attgaccacg ctcatgataa aacccgcctt
     9841 ccaccattct cccattgtgg tgtagcccga accgaaaatc acaggcgaag taccggtcgc
     9901 ataatgagtg agggtcatca taatgttaga tgcggccgcc atcatcagcg cggtcggcat
     9961 cgccggggca ttcagtgaaa cagcagcagc gagaaatgcg ccgaacatag cggtaatatg
    10021 tgcagtagta ctggcaaaca tataatgcgc atacatataa gcaagcacga ggattacgcc
    10081 cgcagccgtg ccgctaacgc ccaaaccgcc gacactttcc gccaacactc cggagaacca
    10141 tttaatcagt ccgagtttat ttaaaaatgc ggccatcata atcaatgcgc caaaccaaat
    10201 aatcgtatcc cacgcacttt tttctttcaa aacatcgtcc caagtcaata caccggaaag
    10261 caaaagcagg cttaatccga taaatgcggt ggcggtggcg ttgatactaa aagcgtgatt
    10321 gccggtaata agggcgggaa catctgccca caacagcaac aagataccga aaatgaccgc
    10381 cataatgatt tcgtctgccg acattttacc catctccctc agacggtctt tggcaaattg
    10441 aacggcattg ggcgtttctt taatttcagg cggatacaaa aaatataaaa tcaaaggcat
    10501 aacgaaaaag gcgataacgc cgggaacagc cattgcccac gcccacgccc cccaagaaag
    10561 acggaaacta ctgcctaaat tttcggcaat caagttgacg attaaagggt tgggggcagt
    10621 tgcagtaata aacatagccg acgaaatggg attggaatga tagttgacca aagccaaata
    10681 tttacccatc ttgccttctg tgccttttgc gggattggag ccgtaactgc cggcaatcga
    10741 ctgcataatc ggatgtataa tgccgccgcc gcgcgcggta ttggaagggg taacgggagc
    10801 cagcagcagt tcggaaagag cgagactgta accgatgccc agcgtttttc ttccaaaaac
    10861 ggcgataaac aaatatccga tacgcatccc cagccctgtt ttgagcaaac cgcgcgaaat
    10921 cataactgcg atggcaatca gccaaatcaa cggattggcg aacgcactca acgcatcgct
    10981 catcgccgcg cccggtttgt cggcggttac gccggttact gcgaccaacc cgacggcaat
    11041 aatcgacagc gcgcccaacg gcatggcctt gccgataatg gcggcaatca caccgacaaa
    11101 catggccagc agcgtccaag cctgaggctt gaccccgtcg ggtacgggca gtgccaaaac
    11161 cagggcgcac aatactgcgg caatggcgag gggtatcggt ttgaaaccca atttcatcat
    11221 attgacctcc gtaaaaaaga ccgtcccgaa aaatcggaaa aataatattt aactaattgt
    11281 tttataagat atattcagat atttcaccgt ctttccgata tgcggctccg ggcaactttt
    11341 gtttcagtat ttgaattttc attagactga atacgccgtt tgaacggcac ggcgaaaacc
    11401 cgggggatgc cggacgttca gtcctttttc gcaccttgaa ggtaaagcat ttccaaggca
    11461 attgtggcgc cggccaaagc ggtaatgtcg gattggtcgt aagagggggc aacttctaca
    11521 acatccatac cgacgatgtc gagatccgtc agcccacgta ggatttttaa tgccctgtcg
    11581 ctgctcaagc cgccgcatac gggcgtaccg gtcccggggg cgaacgacgg gtcgaggcag
    11641 tctatgtcga aagtcaggta aacgggcata ttgccgacgg tttctttgat tttacggacg
    11701 gtctcttcaa cactgtcttc attaacttgg ggggcggtca acacagtaaa aggcaatttt
    11761 ttactgtgtt cggtacgtat gccgatttgt acggaacggg acgggtcgat gaggccttcc
    11821 ttgggggcgg tatagaacat cgtaccgtgg tcgtattcgc tgccgttgtc gtaggtgtcg
    11881 gtgtgcgcgt caaaatgaat cagtgcgagt ttgccgaaat agcgggcgtg ggcgcgcaac
    11941 aacgggaggg taatgaaatg gtcgccgccc aaactcaaac agcgtttgcc ggaagaaagt
    12001 aatttgccgg cgtgcgcttc cattttttcg acaaaatccc tgctgtcgcc aaaagaaaaa
    12061 accaagtcgc cgcaatcaat aatgttcagg cgttcgcgca catcaaatgt ccatggaaac
    12121 ctgcggtgct cccaagcgag gttgacggag gcgcgccgga tggcttcagg accgaaacgc
    12181 gcgccggaac gccctgaaac cgccatatca taaggcacgc cggtaataac ccaatcggca
    12241 tgactttcat acggcataaa attaagcggc aggcgcaaaa acccgaaatt attggaaacg
    12301 agggagttgt cggtttgtcc tgccagtgtg ctgtattgca tcgtaatgat tccttgtaat
    12361 tggtttcaat cggtcgtgat gattggtgtt tgagtaagaa aatcgggctt cagacgacat
    12421 atccgatgcc ttgatgcgtc tattcgtctt ccaaataggt ataaccatta agccccgctt
    12481 cgagttcttt taagaaagac atagcctgcg aggcaggaag gtctgaatgt tcgatttgtt
    12541 cgcgatagcg tttcatcagc tctttcggat cttgataaac gtattcgagc atatcggcaa
    12601 cggtgtttcc ttcatcgtaa tcgatgacgg taaattgtcc gtcttcccct acaacaacat
    12661 cggcagtggc agtgtcgccg aaaagattgt gcatattgcc gagtatttcc tgatatgctc
    12721 ccaccataaa aaagcctaaa agcggcggct cttcttcggg ataatcaggc ataggcatcg
    12781 taccggcgat gccgtctccg tcgatgtagt ggtcaatcgt accgtctgaa tcgcaggtaa
    12841 tgtccaacaa cacggcgcgg cgcgcaatcg gttcattcaa accggtaatg ggacaaacag
    12901 ggaaaagttg atctatgccc caagcatcgg gcaaagattg gaagagtgag aaattgacat
    12961 acagcttatc ggcaaaacgt tcttgcaatt cgtcaataat ggttcggtga gaccggtgtt
    13021 tttcattaaa caattcgccg acttcatgac agatatttaa atacagttgc tccgcccacg
    13081 cacgttgcgc caaactcaac agcccgacat tatactgatt atgcacatcg gcaagatcaa
    13141 actgcccttc gtgtatccag ctgcgtaagg aacgtttttc ccgcgaggcg gaaatatccg
    13201 tccaagtttc ccacatactg tgcaacacac gcggtgcttc gggcgatggc gcatccagcc
    13261 ggcgcggttt gtaacgttca acgcctataa cattagcaac caaaacggcg tgatgtgcgg
    13321 taatgccgcg cccgctctcg gtaatgattg tcggatgcgg cagcccgtgt tcgagacaag
    13381 cctggctgat gccccatacg actgtggagg catattcgtt gaggctgtaa ttaacggaac
    13441 aatccgattg tgtgcggttt ccttcgtaat ccacgccaag cccgccgcct acatcaaaac
    13501 agcggatatt tacccccagt ttgtgcaact caacataaaa ccgagccgat tcgtgtacac
    13561 ctgtggcaac atcacggatg ttcccaagct gcgagcctaa atggaaatgc aaaagctgca
    13621 ggcagtccag cctgtttttt tgtttcaaaa tatcgaccag ttgcaaaact tgggaagccg
    13681 acaagccgaa ttttgatttt tccccacccg aagactgcca ttttcccgaa ccttgggaag
    13741 ccagtctggc gcgcacaccc aaacggggct tgatgccgag tttttccgcc tcttccaata
    13801 ccatttgtat ttcggacagc ttctcaatca ccaaataaac ctgatgcccc agtttttcgc
    13861 ccatcaaggc gaaacggata tattcacggt ctttatagcc gttgcagacg attaatgttt
    13921 gccgggtgcc ggcgtgtgcc aaaaccgcca tcagttcggc tttagaacca gcttccaaac
    13981 catgcggttg tccgcttgac ataagcgatt cgatgacgcg gcggtgttgg ttgaccttga
    14041 tagggtaaac caaacaataa ccgcccttat agccgcactc ttcccgtgcc gtctgaaagg
    14101 cgcggttgat gtcgcggagg cggtgttcga ggatttgcgg aaaacaaaac aaaacaggca
    14161 ggcgcgcctg atgtttttgt tgcacggctt cagtcagttt ttgcagtgaa acagtttgat
    14221 tgtgttgcga gggattgggg cggacgatga tttcgccgga atcgtcaaca tcataataac
    14281 ctatgcccca atgattaatg ttgcacactt cacggatggt aaggataggc ataataaacc
    14341 tgctccgtct gtcgtgttga aaaggaatga ttataacaaa tcagcgtgaa atgtcatttt
    14401 tttaataaga aaagcctgcc tcatacctga tgaggaacag gcaaaatgcc gtctgaacgc
    14461 ttcagacggc attttggttc atctttccat caaaggagtc agcgatcgag ctgctctttg
    14521 atgattttca ggtcggggta agacaacacg atgtcgtatt cgcggccgtc tttataggct
    14581 tccacttcca gcacaggctt accccaatgg tcgtcggcat cgacatcgtg aacctgataa
    14641 ccgcgctgtt ccaacatttt cacagctttt acgcggttct gttcaaaatt gggatcactg
    14701 taaatctgac gctcggcaga gtcgctggca aatgcggttg cggcactcag ggaaacaacg
    14761 gcagccaata acagtttttt cattttcagt cctttcttta tcggttgatt gaataagatg
    14821 tgtttttcaa tgccgccatt aaaacacagc aaaattaggt ttgaattaga gggaagtcaa
    14881 gaagcgtaaa tgccgtctga aaaaaacaga gccgtcaaac ggctccgttt tccttatgct
    14941 tctttggttt cagccgcctt ttggcgcagg cgcaggctca attcacgcaa ctgcttgtcg
    15001 tccacgctgt tgggcgcgtt ggtcagcagg cattgggcac gttgtgtttt cgggaaggca
    15061 atcacgtcgc ggatagattc ggcgccggtc atcagggtaa ccaaacggtc gaggccgaat
    15121 gcaaggccgc cgtgaggagg cgcaccgaat ttcaggttgt ccaagaggaa gccgaatttc
    15181 tcttgttgct cttcagggct gattttcagc gcggcaaaca ctttctcttg tacgtctgca
    15241 cggtggatac ggatagagcc gccgccgatt tcccagccgt tcaataccat gtcgtaggca
    15301 cgggccaggc agtttgcagg gtcggaaacc atcaggtctt catgaccttc tttaggcgcg
    15361 gtaaacggat ggtgtacggc aacgtagcgg tcggcttctt cgtcgtattc gaacattggg
    15421 aaatcgacaa cccacaaagg tttccattcg tctgtgaagt agccgttgtc tttgccgtgt
    15481 tccaagccga ctttgatacg caatgcgccg atggcttcgt tcacaacttt ggttttgtcc
    15541 gcaccgaaga aaataatatc gccgttttgt gcgccggtac gctcgatgat ttctttcagg
    15601 gcattctcag acaggaattt tacgattgga gattgcaggc cgctgtcttc gccgtttgag
    15661 aggttgccca catcgtttac tttgatgtat gccagacctt tcgcgccgta gatgccgaca
    15721 aatttagtgt attcgtcgat ttctttgcgg ctgaattttg cgccgttcgg tacgcgcagg
    15781 gcgactacac ggccgccttt catatctgcc gcgccacgga agactttgaa ttcttccgtt
    15841 ttcatcaggt cggtcaactc ggtaaatttc aagttgatac gcatatccgg tttgtcagag
    15901 ccgtagtaga acatggcttc ggcgtaaggc atacgcggga agtcgcctaa atctacgttt
    15961 aaagcatctt tgaagacttg tttcgccata ccttcggtga tgtccatgat ttcatcctcg
    16021 ttcaagaacg aggtttccaa gtcgatttga gtgaattcag gttgacggtc ggcacgcaag
    16081 tcttcgtcac ggaaacattt ggtgatttgg tagtaacggt cgaaaccagc aaccatcaac
    16141 agttgtttga acaattgcgg agattgcggc agagcgaaaa actcgcccgg atgaacgcgg
    16201 ctcggcacga ggtagtcgcg cgcgccttca ggcgtggagc gggtcagcat tggggtttcg
    16261 atgtcgataa agccttgtgc gtccaagtag cggcgaacgc ccatagcaac ttggtaacgc
    16321 aggcgcaggt tgcgttgcat ggcaggacgg cgcaagtcga taacgcggtt ggtcaggcga
    16381 acgttttcgc tgatattttc atcgtcgatt tggaacggag gtgtagcggc agcgttcaag
    16441 acttcgattt ctttggcaag aatctcgatt ttgccggaaa tcattttatc gttggtcgtg
    16501 ccttcgggac ggttgcgtac gcggccggta atgctcaaaa cgtattcgtt gcgggaggaa
    16561 tcggcagcgg caaacgcttc gggcgtgtcg ggatcgatca cgacttggac gatgccttcg
    16621 cggtcgcgca ggtcgataaa aatcacaccg ccgtggtcgc gtcgacggtg tacccagcct
    16681 ttgacggtaa cggtttggtc taagtattgc tcactgatca ggccgcaata gttggtacgc
    16741 ataaaatcac cttttattga tttaaactga aaacagaaaa tgccgtctga acggcggctt
    16801 tattgttgtt cgggcaaatc cgccttttca gacggcatag gtcctgccaa tgttttgacg
    16861 ggcaggtcgt cagggatgac catacccagc gaaatcacat atttcaatgc ttcgtccacg
    16921 ctcatatcga gttcgcgcac atcgcttttc tttaccataa tatagtaacc gccggtcgga
    16981 ttcggcgtgg tcggaacata cacggaaaga taatcgccgt ccttcggcaa tgcggcctta
    17041 accgcattcg acacctgccc tgacacgaaa gcaatcgtcc aaataccggg ctggggaaac
    17101 ggcacgagta ccggcgtttt aaacgaacgg ctgctgtcgg acagcagcga ttcggatact
    17161 tttttcacac tcgaatagat ggatttcaca accggaatcc gccccaacag gctgtcccac
    17221 gcggcgagga tctgccgacc caatacgttg gcggcaaaca atccggttac aaacaatacg
    17281 gcaatggcaa cgataacgcc cagccccggg atattaaacc ccaaaacata ttgcggccgc
    17341 cattgcttcg gcagcaggtt gacgagctga tcggacgcgg aaacgatata ggaaaccacc
    17401 caaaccgtta ccgcaatcgg cagccagacc aaaatgcccg taatcagata tttttttaac
    17461 gccttggcag ctttgccgcc ttcggccgca ggttccgtca tcttgcttga ttccgacaaa
    17521 gtccgtacaa accgcacatt atacgcgttt gcccggattc aaacgaaatt tttatcccgc
    17581 cccgccaaac cgccggcggc ttcagacggc acggcaactt gatataccgt ctgaacacgc
    17641 ggttcagatg ccgtccaagt cgttgaacat caacccgata ccgataccgt tctgcttgtg
    17701 gttgtagtcg atcaggctct cgccgtaacc gtggaatccg cgtaccacgc ctttgagttt
    17761 gcccttaatc ggaaacgtgt aggcggcttc aatcgcgccg tagcccgttt tggggttgta
    17821 gcgcaatacg gaatacacat tctgcctgtc gttcaggcgg tactgcagct tcacgtcgcc
    17881 ataccccata tagtcggcaa tatcgggatt gtcgttttta tcgccgctct gatcgaacgc
    17941 acgcacccac acgcgcggaa tcaccgtcaa tttgccccat tccatgcctg ccatggcgta
    18001 aatcctgttc cacgaacgcg attcgggacg gctctgtccg ttggactggt ggacaaaacc
    18061 cgcaccgagc atacgcagcc tgccgccgaa cggcaaatcc gccttcacag gctgggtcag
    18121 gaaaatttca ggtttgtaat ccgtattgcg gaacggcgcg gatttcctgc cttggttgta
    18181 aatctgccaa tcggatcttt gggtgtagcc gaaccacaga tccgcgcggg ttttaaacaa
    18241 atcttcggca attttgcttt tgaacgaaac ctgcaatttg gtttccgcac gtttctgctg
    18301 tccgaatttt tcctgtacag tcgtaccgcg cgtcggcgaa cccggggcat agttgggcga
    18361 attgttgtac cagagcggca taaggtacat cggattgtgt tcgcgtacgc ccaacagccc
    18421 gcgcaaatcg tttttgtcca agtcgtacat caggctcaaa ggcgtataga tgtcggcggt
    18481 ttcgcccgca ctgtcggcag gaagcgcatc cccgcctttt tcaacaacaa tgaccgcctc
    18541 gcccttatcc aggctgctgc ggacggtttc cgtcagattg agtacggctt tcgactcctg
    18601 cccttcctgc cctgccgaag acggaagctg tgccgcaaaa atcctgtcgt aacacgccaa
    18661 acgcgtaaca ttgtccgtca aagcggcgca ttgcagcgcg gtctctccaa aagcggatgc
    18721 catcggcaac agtcctgtca aaagaatata gcgcatattc cgtgtattca tctccgcccc
    18781 cattgtcggc atattggttt tcaaacggca ttttatagcg gattcggata aaaaatcgca
    18841 ccctttccgc catttcggga ttttgccccc gcaatacaga aaaccccgaa accgtcgggc
    18901 ttcagggttt tccgcttatc gcgtatcaac cgccttggcg gttttgcaaa aatcaagcca
    18961 aggcttttac ttttgcagac agacggcttt tgtggcgtgc cgctttgttt ttgtggaaca
    19021 cgcccttgtc ggcgatgcgg tcgatgactt tgacggactc ttggtaaacc gcttgtgcgg
    19081 cagctttatc gcctgcttcg actgctttca atactttttt cactgcggtg cggaatgcgg
    19141 tacgcaggct agcattgtgg gcgcgttgtt tgacggactg gcgggcacgt ttgcgtgctt
    19201 gtgcgctgtt tgccatattg gaatctcctg gaagaaaaat aaattctgta aacgcgcaat
    19261 tttaaagggg gtttacatta tatgcaagcc ttttctctct atccgcccgt cggacaccct
    19321 gacagcgcaa aaaaacggca aaatcgggcg gacgtttttc tccccttttg gaaacaggat
    19381 aattttttag gaaaatcagt ccaatatcgc tttgagtttg cacaaattta ctttacaatg
    19441 cccaaacctt gcgccggggt aacgatgggg cgcggggatt tctttttttc caacctcttt
    19501 catcaggaat ctgcccgtat gaaaaaatcc gtattagccg ttttggccgc gctgtctctg
    19561 gcagcctgcg gcggcagcga aaaaaacgcc gtgcaacctc aagcagacgc tgcgtctgct
    19621 gccaatgccg aagccgctgc caccgatact ctgaacatct acaactggtc gaactatgtt
    19681 gatgagagta cggtcgaaga ctttaaaaaa gccaacaatc tgaagctgac ttacgacttg
    19741 tacgaaaaca atgaaacgct ggaagccaaa atgctgaccg gcaaatccgg ttacgatttg
    19801 gtcgtgccgg gcatcgcctt cctgccgcgc caaatcgagg cgggcgcgta tcaaaaagtc
    19861 aacaaagacc tgattcccaa ctataaaaac atcgatcccg aactcttgaa aatgctggaa
    19921 accgccgacc cgggcaacca gtatgccgtc ccctatttct ccggcgtgaa cacgattgcg
    19981 attacggcga agggcaaaga gcttttgggc ggcaagctgc ccgaaaacgg ctgggatttg
    20041 ctgttcaaac ccgaatacac ccacaagctg aaatcctgcg gcatcgccct gtgggacacc
    20101 ccgagtgaaa tgttcccgat tttgctgaac tacttgggca aagaccccaa aggctcgaat
    20161 cctgaagact tgaaggcggc ggcggaagtg ttgaagtcta tccgtccgga tgtcaaacgt
    20221 ttcagcccgt ccatcatcga cgagctggca cgcggcgaca tctgcctggc ggcaggcaac
    20281 ggcggcgatt tgaacttggc gaaagcacgt tccgaggaag tgaaaaacaa cgtcggcatc
    20341 gaagtgctga caccgaaagg tatgggcttc tggattgagt cttggctgat tcccgccgat
    20401 gcgaaaaacg tcgccaatgc ccacaaatac atcaactaca cgctcgaccc cgaaatcgcg
    20461 gcgaaaaacg gcatcgccgt aacctttgcc cccgccagca aaccggcgcg cgaaaaaatg
    20521 cctgccgagc tggtgaacac ccgttccatc ttcccgaacg agcaggatat gaaagacggt
    20581 ttcgtgatgc cgcaaatgag cacggatgcg aaaaaactgt ctgtcagcct gtggcagaaa
    20641 atcaaagtcg gcaccaactg atttgaagca ttaaaaatgc cgtccgaacg atgttcggac
    20701 ggcattttat attggattga aatagaaata tttatatagt ggattaacaa aaatcaggac
    20761 aaggcgacaa agccgcagac agtacaaata gtacggaacc gattcacttg gtgcttcagc
    20821 accttagaga atcgttctct ttgagctaag gcgagccaac gccgtactgg tttttgttaa
    20881 tccactatac cgtcgttccc acggtcagga tttcagattg cggacatctg tcagaaaaga
    20941 caaaaaacct tccgccgtca ttccctacag gcgagaatcc gatccgttga aattcggttg
    21001 ttttaaataa attcttgcag ctttgatttt ctgtttttcc gataacgccg taactttgaa
    21061 acgcgaaagc ggtaatccga tccgttggga ttttgcaact tcaaatcaat ccgcaaactg
    21121 aaatcccgtc attcccgcgc agtcgtgaat ccgaacgcgt ccgcacgaaa acctgcatcc
    21181 cgtcattccc acggaagtgg gaatctagga cgtaaaatct caagaaaccg ttttatccga
    21241 taagtttccg caccgacaga cctggattcc cgcctgcgcg ggaatgacga aatttcggcg
    21301 agccgtaggg tgggctgtag ggtgggcttc agcccaccaa tttcaccgca tcaaaggttt
    21361 ttgggaatac ggcggtttcg gttttcggtg ggaatgggcg gaatggataa cattgacgga
    21421 gttgggggaa taggtggaaa cggtgggatt ggtgggctga agcccaccct acagccagcc
    21481 ctatgtccac gctgcacatc ctacgcccgc cttgcgtttc ttataagcag cattttacgc
    21541 aatactttgc gtccgctgaa aaaatttcaa acggtctttt ttatcaaatg cgaaaaatat
    21601 aacgtcattc ccgcgcaggc gggaatctag tctgtcggtg cggaaactta tcggataaaa
    21661 cggtttcttt agattttacg ccctagatcc cgtctgctcg ggaataacgg gatttgaggt
    21721 ttctgttttt gattttctgt tttcgaggaa ataacggttt agaagttacc cgaaacctca
    21781 aaaaaaccga aaccgaacgg actggattcc cgcctgcgcg ggaatgacgg gattcgagat
    21841 tacggtattg tcggggacga tgggaaatgg tgggaattgt gtaaaaaatg ccgtctgaag
    21901 cctttcagac ggcatttgcg gcgtttggac gtttaaaact tcatttccaa gctaaatgtg
    21961 tagtttcggc cgggggcggc atatcggttg taaacgccga catttttgtg ttggttgact
    22021 gcgccgccgg cagtttgccg cacattttcc caagtaacat agcggtagtt gaggaggttg
    22081 tacacgcccg cacggagggt gaagtgtttt ttaatcgtgt aataaccgga cacatccaca
    22141 atataccaag ggcgggtacg gcgcgcggtg gcttttgtat tgcggctgtt gccgttgagc
    22201 aaagcacggc tgcccaacaa ctctgtgatt tccttggctt tggaataagt cagcataccg
    22261 ttcacacccc atttgccttc cggttggtca tagcccaagc cgacgacata gcgcgagggt
    22321 tggatggcat caaacaggtg tgattgaata tcggtgcggt ctgcgcgttt tttgatgtcg
    22381 cggacacgga cacgattata ggcaaatgta gaataccaac cttcgggcaa tttatcccat
    22441 acgccgttcc aatcgatttt gcccaaaaca ttgatgccgg taatccgcgc gctttgggca
    22501 ttgaggtaag ccgggtcgcc tttggctgtt tctttgccgt ctttaatttg cgcttcataa
    22561 ccccggacaa tcaaatcgcg gtaggcattg ttgaaccaac ttgcctccaa gttgccgaaa
    22621 tcgcctttaa acacgatgcc ggcttctttg ttgaacgatt tttccggatc gattttgacc
    22681 gccttgcttt gaacacccgc ccgccagccg tacatttccg caaacgaggg caggcggaag
    22741 ccagttgaag tgcggtaagt caaatccagc cagtcggcag gtttgaggac gatgcctgcg
    22801 ttccaggaca gggtgcggtg cgtaccggtg gaaacgctgc cgtcgtccga atgcgtgctg
    22861 cggtagtcgt agcgcaaccc cgcgccgaca tccgcccacc tgcccaaacg gacattgtcc
    22921 cgaactgccg cgtaatagct tttgccgttg atgctgcgcg gcgtgcagtc cgtataagta
    22981 ttgttgccca agcggcagat ttgctccgta accacatttc ccctgcctat gctgacccaa
    23041 tagggagtgc ttgtgctgcc gttggggctg attttttgat tgccgttttt agggggcgtt
    23101 ttcaacaaat aggcgcgttt ggcattttga aaataataat cctgatggcg gagattagag
    23161 ccaaagcggt caaacccgag attcacgctc aggttgtggc ggattttggc ggtatcgaag
    23221 gattttttga atgccgcctg caagagcctg tggctttccc cgtaaatcac gcggtcggat
    23281 ttgtaatagg aaaacggctt gtcggcactc ggacggcaat atttgtccga accgtcggca
    23341 gaacagtgcg tctgctgaaa atgattgtcc aaaccgatgc cctgccggtc gtaagagagg
    23401 cgggcataat ccgcccaagt gtctttatcg gcattggtat agacatattc caaaccgtag
    23461 cggcttttgg tgtgcgtctc gtcgtaaaac acgcccgtac cgtattccgc gcccaccagc
    23521 gcaccgtttt caccgttggt aaacagaccg ccgtatttgt ggttgcccgc gtatttgccg
    23581 ttaccgggca aagaacccgc ctgttttgaa tttgcatcaa aaaccgcctt ggtcaggaat
    23641 gccggaaccg tcatatcgcg cgtgtcgaaa gtttgttgcg tgtgttcgag tatgccgccg
    23701 atgtagtgcc gcttattctc aaaacgaaaa cccgggcgga acagccacga tcggctttcg
    23761 tatgaaagcg gatcggcgag gaagcggttg ggacccgtgt agtctcgggt agaaaccgtt
    23821 tgacgttcgt ctttgccgac aacatctttt ttcggtttgc ttttacactt ttcataactc
    23881 ccgctttcgc attcttcttc gaccacgaaa tgagcgtatt ggttgtcgtc ctcaaccggc
    23941 accagcctgt taaagctctg aacgccgcgt ccggcatctt cgtgggcgcg gatttccccc
    24001 gcgcgccgcc cggtgtggat cagcaaagcc tccgcaccgc cgatgcgccc cgccagcgcg
    24061 atggattggg taagcccccg gtttttgccg gaataggcgg ttttactctg aatgccccac
    24121 tgcctgcctt ccccgataac atcgtcggcg gttttggttt gaaatgcgac cgaacccgcc
    24181 aatgcgccgc tgccttgttc gaccgagttt gagcctttgc tgatttcgac agccttgacg
    24241 ttttcatact cgatttcatt gattgcgccg ctgctgcccg ccgtcctcgt cccgcccaat
    24301 gccgcctgcg cggtgtagga ctgtatttgc gccaagccgt ccaccgtcaa ggaaacgcgg
    24361 tttttgtcca taccgcgtat cgagtagccc gagcttgcgc cgcgcccctg ttcgacgacg
    24421 gcgatgccgg ggtcgtagcg agtcaggtcg cggatatcga gtacctgttc cttgctgagg
    24481 gtgtcggcgg ttttgaccaa tttgcccagg ccggttactt cgttatcgcg gcgggttttc
    24541 tgttttttgg cttttacctg tatggtatcc aactgttttt cctgtgcttg tccggcttgc
    24601 acattttctg cataaacggg cagcgcggtc attaaagaca ggcataaaat atttaatcgg
    24661 aacaaatgtt gctgttgcat agtgtttccc taatcttcgc tttcagacgg catcggaagg
    24721 agcgatgccg tctgaagcct tattcttgat tgttcggcaa ccgtgcttat tgcacaggct
    24781 gttggcgttt cgcaccgaat acgacagttg cactgcttgc tgaatttcca ttgccggatg
    24841 catctgttgc atttttcgtt tgtttatcgt ccgaataggc aaaccatccg cccaactctt
    24901 cggctttggg cccgtaaaaa ccgccctgca ccttggcatc tgtgatatat gccttaggcg
    24961 tgcgggtggt atttttttga tcgagatcaa aacctaattc agcagttttt gccgtaccgg
    25021 aaaaaccgtt gccctcaatc ttaccatcaa tggtaaaggt tgccgcctgc ctgttttcag
    25081 cggttaacgt gccggtaatt tttttctcgg caaaattcac agtaaattcc gccctgttgc
    25141 cgccctcttt atcagaagca ttgccgctcc agcttgtgcc gttggcaata tgcccgtacc
    25201 aagacccccg ataaacgacg ttttggtcgg ttggaatctc tttttcatcg gtgcgctcgc
    25261 cttggaggaa catactttgt tcaacttgtt ccgttttagc atcagcttga ctactgtttc
    25321 ctcctgcctg catcgcggac ttgctgtttt tacgcatcaa cattccgtat ttcagataat
    25381 tgaggttgga acagcagact tcaacttcat aggttttcgt tcctgcctgc ccgccgttaa
    25441 cgctctcagt acccgaagca gcttgcgcgc cgcccgcgcc tgtttgggct tgggtgtctt
    25501 ttttatcact ttccggcgtg taggttgttt tgtaggtaaa gtctgtcccg ccgtttttac
    25561 cgtttttacc gtttttactt ttatctgcct gattgccacc gctttcggaa tccttgggca
    25621 ggagcggaat cataatgccg tcgacaacca gttgggcggc gttgctgaag ttgtcgagat
    25681 ttttgacttc cttatcgcca gatttcagct caaccgcatc caaaaccgtg gtcagcttac
    25741 cgttttcaga caacgtgcct gccgcaccgt ttgatgctgc cgcatctgtg ctgcctgaag
    25801 ccgccgcgcc attttccagt ttgtctttgg ttttcgcgct gccgacaacg gcaacttttt
    25861 gatcgtcgct caaaaagcgg aaacccaatt cctcaccctt cggaccgaaa aagccgccgc
    25921 tcaaagaaga cgagtcggaa acaaagggat gttgtttggt ttcgccgttg ccgggtttgt
    25981 cggttgccgt tgccttgccg ttaaagcggt tgcctgttac ttgagcctca aggctgtagt
    26041 attgggtggt ggcttggttg ttactggtat tcgcattgtt gcgtatcaat ttacccgtca
    26101 atttcttatt gtcgaaatcc acttctaaat tcgaggtaaa tccataaccc tcgtgaccgc
    26161 tttgtaaagt cgtttcattt ttattggaat attcctcgcc gtcatcaccc gaaaatccgc
    26221 tatacctgtc gccttgactt tttgaaggtt ggataatttc acgaaaattt tgagattttt
    26281 tgacatcggt cgcaaaatgc cacacacctt tataggtaat ttttccagaa gcgggaagtt
    26341 gtcgggaagg ttctttaccg tgatagaaga tataaccgtc gtcgccttgt tgtacaattt
    26401 tatttgtcaa atcaagatgt tgtttagcgt gtttgtaaaa ccaaccggaa taaacatatt
    26461 ggaaattttc gtgatttgtt gccttatttt taggttgatt tacaccgttg ccagcgctgc
    26521 cgctttgatg gtttgatggt gtgagatagg gggaagaata aatattgctg tcgtcgtctg
    26581 tttctacttt ttcaataacc gatttttgcc gtttagggag ttccttgggg tctgtcggca
    26641 atcctgtcgc ctcccaatca ctctcgttca gtttaacctc gttttctttt gccgacggat
    26701 accaattcct ccgtttcaac ctcatcgcaa aaccgtatcc gccttggtct ttttgggctt
    26761 gcggtttttc ggaagaaaca tcttgatact ttggcgcggg acgcggggct tcggtatcga
    26821 cagaatcaag atcgaaactg ccgcctccgc ccaaacaagc actcaacaaa aacacaggca
    26881 gcaccatagc agcctgattc accaatggat tgttcatata aacccaattc aattaaagaa
    26941 tgataaagat tattatttta tttattttta acaaatttgc aaatgctttt ttattttttt
    27001 agggaataca ccaaaatccc gtcatttccg cgcaggcggg aatccgaacg cgtctgcacg
    27061 gaaacctata tcccgtcatt cccacgta
//
LOCUS       DAWFHY010000019        26510 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.019, whole genome shotgun sequence.
ACCESSION   DAWFHY010000019 DAWFHY010000000
VERSION     DAWFHY010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 26510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26510
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.019"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(13..1308)
                     /locus_tag="WGQ68_000728"
     CDS             complement(13..1308)
                     /locus_tag="WGQ68_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEZ0714572.1"
                     /translation="MIRPDFQEYLPSYYFSSVNPHTVYPKLQCRLKTDTCIIGGGLSG
                     LCTALPLAEQGHETVVLEAARIGFGASGRSGGQVISDYACGMEEIEKQVGLEQAQWFW
                     QQSLQAVELVDERVRKHAIDCDWQRGYATVAIRSQHWEELQQWHEHAQKYYGATHYQL
                     WDNTELKQQLDSDMYQGAQFDPLSGHLHPLNYTLGIARAAAEAGTKIFEHSPMIRIEP
                     YQNGWLVYTPEGSVECKNLVYAVNTYAGLNPIFKPLERKAIAVSTFIIATEPLGARAK
                     GLIRNNMAVCDNRHILDYYRLSADGRLLFGGKDNEFIDNPARMTELVRQDMLKVFPQL
                     ADVKIEYSWGGECDITANLVPHFGRLAPNVFYTQGYSGHGMAITGIAGLAVAEAILGD
                     ECRLKPFERLRQPNIILQPFLRKLGSFLGSKYYQWKDSR"
     gene            complement(1434..2321)
                     /locus_tag="WGQ68_000729"
     CDS             complement(1434..2321)
                     /locus_tag="WGQ68_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0714573.1"
                     /translation="MQKSKLSWFLKLMLALSLAFLYIPLVVLVIYSFNESKLVTVWGG
                     FSTKWYGALLENDTILEAAWLSLRIAVVSSLAAVVLGMLAGYAMARIKRFRGSTLFAG
                     MISAPMVMPDVITGLSMLLLIIQVQIFLQGSEWLQHLYFDRGFFTIFLGHTTLCMAYI
                     TVVIRSRLVELDQSLEEAAMDLGARPLKIFFVITLPLIAPAIASGFLLGITLSLDDLV
                     ITSFLSGPGSSTLPQVIFSKIKLGLDPQMNVLATILIGIIGTLVIIVNYWMMRQATKR
                     DREAAEAYRQEKLAAEKAN"
     gene            complement(2321..3286)
                     /locus_tag="WGQ68_000730"
     CDS             complement(2321..3286)
                     /locus_tag="WGQ68_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEZ0714574.1"
                     /translation="MNLNKLKNKLFRRPGQRVVIAVPYIWLLVLFLIPFAIVLKISFA
                     EQEIAIPPFTPLTSIDEDLGRLNIAISYQNYADIFQNFWHTLNPFGDSENSNIYLMTY
                     WSSIKTALMTTVICLLVGYPTAYAISRANPSVRNGLLLAIMLPFWTSFLLRVYAWMGL
                     LGHNGIVNNLLIKMGIISEPLDLFYNAFSLNLVMVYAYLPFMILPLYTQLVKLDNRLL
                     EAASDLGAGPVKSFLTITLPLSKTGIIAGSMLVFVPAVGEFVIPELVGGSENLMIGKV
                     LWQAFFDQNNWPLASAVAVVMVALLVVPIALFQHYENRELEEGAK"
     gene            complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGQ68_000731"
     CDS             complement(3302..4426)
                     /gene="potA"
                     /locus_tag="WGQ68_000731"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714575.1"
                     /translation="MTATTASSAKPYLKIQGLVKKFGDNYAVDNIDLDIYQHEIFALL
                     GSSGSGKSTLLRMLAGMESPNQGKIILDGQDITKLAPYDRPINMMFQSYALFPHMTVE
                     QNIAFGLKQDKMPKGEIAARVEEMLRLVQMTKFAKRKPHQLSGGQQQRIALARSLAKR
                     PKILLLDEPLGALDKKLRQQTQLELVNTLEQVGVTCIMVTHDQEEAMTMATRIAIMSD
                     GQLQQVGTPSDVYDYPNSRFTAEFIGETNIFDGVVIEDHADYAVIECEGLENHVRIDH
                     GLGGPSEQDLWVSIRPEDIDLYKEKPEYLGDYNWAKGTVKEIAYLGSFAIYHIKLANG
                     RVVKSQVPAPYWYVRNITPPTWDETVYISWPENQPTPLFR"
     gene            complement(4621..4728)
                     /locus_tag="WGQ68_000732"
                     /pseudo
     CDS             complement(4621..4728)
                     /locus_tag="WGQ68_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="WGQ68_000733"
     CDS             complement(4807..5076)
                     /gene="rpsO"
                     /locus_tag="WGQ68_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEZ0714576.1"
                     /translation="MALTVEQKAQIVKDFQRKEGDTGSSEVQVALLTFRINDLTPHFK
                     ANPKDHHSRRGLLKMVSQRRRLLAYLRRTQPDTYRALITRLGLRK"
     gene            complement(5299..6234)
                     /gene="secF"
                     /locus_tag="WGQ68_000734"
     CDS             complement(5299..6234)
                     /gene="secF"
                     /locus_tag="WGQ68_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEZ0714577.1"
                     /translation="MELFKIKRDIPFMSYGKLTTFISLVTFIAAVFFLVTRGLNFSVE
                     FTGGTVMEVQYQQGADVNKMRERLDTLKIGDVQVQALGTNKHIMIRLPNKEGVTSAQL
                     SNQVMDLLKKDSPDVTLRQVEFIGPQVGEELVSNGLMALGFVVIGIIIYLSMRFEWRF
                     AVSAIIANMHDIVIILGCFAFFQWEFSLTVLAGILAVLGYSVNESVVVFDRIRENFRK
                     PAMRGHAVPEVIDNAITATMSRTIITHGSTEAMVVSMLVFGGAALHGFSMALTIGIVF
                     GIYSSVLVASPLLLMFGLSRDNIGKEPKKKEEIVV"
     gene            complement(6238..8091)
                     /gene="secD"
                     /locus_tag="WGQ68_000735"
     CDS             complement(6238..8091)
                     /gene="secD"
                     /locus_tag="WGQ68_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEZ0714578.1"
                     /translation="MNRYPLWKYLLIVFTIAVAAVYSLPNLFGETPAVQVSTNRQAII
                     INEQTQSKVDAALQNAGIQTDGMFVVDNSLKVRFKDTETQLKARDVIENTLGEGYITA
                     LNLLADSPEWMAKIKANPMFLGLDLRGGVHFTMQVDMKAAMQKTFERYSGDIRRELRR
                     EKIRSGTVRQAGNSLTVPLQDAGDVQKALPQLRKLFPEATLNSDGSNIVLTLSEEAVN
                     KVRSDAVKQNITTLHNRVNELGVAEPVIQQSGADRIVVQLPGVQDTAKAKDIIGRTAT
                     LELRMVEDDPAKLREALEGNVPSGYELLSSGGDRPETLLISKQVELTGDNINDAQPSF
                     DQMGAPAVSLSLDSAGGSIFGELTAANVGKRMAMVLIDQGKSEVVTAPVIRTAITGGR
                     VEISGSMTTAEANDTSLLLRAGSLAAPMQIVEERTIGPSLGKENIEKGFHSTLWGFAI
                     VAAFMVVYYRLMGFFSTIALSANILFLIGILSAMQATLTLPGMAALALTLGMAIDSNV
                     LINERIREELRAGVPPQQAINLGFQHAWATIVDSNLTSLIAGIALLVFGSGPVRGFAV
                     VHCLGILTSMYSSVVVFRALVNLWYGRRRKLQNISIGSVWKPKAEMAGGKE"
     gene            complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="WGQ68_000736"
     CDS             complement(8303..8569)
                     /gene="yajC"
                     /locus_tag="WGQ68_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEZ0714579.1"
                     /translation="MNQAVAQFAPLVLIMVVFYFLIMRPQQKKFKAHQAMLAALKVGD
                     KVVLAAGFKGKVTRVGEQFFTVDIGQGTKIEVEVERNAIAAKVD"
     gene            8697..9806
                     /locus_tag="WGQ68_000737"
     CDS             8697..9806
                     /locus_tag="WGQ68_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HEZ0714580.1"
                     /translation="MSLTRLILKFYALLRLFLGKNARTAWISHPACTGHEPGANHPDS
                     PDRILCIEQALRRAGIWQHLQTIEAEEISDTRLALAHTSKYLNRLESRLPEDGKICRL
                     DDDTVISKNSLSAARFSAGSAVQAVDMVMNRKAWHAFCAARPPGHHAGSGKAGGFCLL
                     NNVAAGAMYALAEYRLNRIAVIDFDVHYGDGTAEIFKDDPRILFFNLFETDLFPFPEN
                     NDMPDGGNMVHLPLPPGTGSRTFREAVRRQWLPRLAAFKPELVLLSAGFDAHRLDESG
                     RLNLHEADFAWLTHKIIQTASGCPGKIVSVLEGGYTLEPLAQSAAEHIRVLAGLGKSD
                     AATAYQKTLDPTKKRFAKPKTGQVRQPTQSDRYDI"
     gene            10169..11233
                     /locus_tag="WGQ68_000738"
     CDS             10169..11233
                     /locus_tag="WGQ68_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-butanediol dehydrogenase"
                     /protein_id="HEZ0714581.1"
                     /translation="MKAARFYDKGDIRIEDIPEPTVAPGTVGINVAWCGICGTDLHEF
                     MEGPIFIPPCGHPHPISGESAPVTMGHEFSGVVYAVGEGVDDIKVGQHVVVEPYIVHD
                     DVPTGSGSNYHLSKDMNFIGLGGCGGGLSEKIAVKRRWVHPISDKIPLDQAALIEPLS
                     VGHHAYVRSGAKAGDVALVGGAGPIGLLLAAVLKAKGIKVIITELSKARKDKARESGV
                     ADYILDPSEVDVVEEVKKLTNGEGVDVAFECTSVNKVLDTLVEACKPAANLVIVSIWS
                     HPATVNVHSVVMKELDVRGTIAYCNDHAETIKLVEEGKINLEPFITQRIKLDELVSEG
                     FERLIHNNESAVKIIVSPNL"
     gene            11374..11697
                     /locus_tag="WGQ68_000739"
     CDS             11374..11697
                     /locus_tag="WGQ68_000739"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HEZ0714582.1"
                     /translation="MGDSVLSAIQQTITQRKSANPSESYVAQLLHKGEDKILKKVIEE
                     AGEVLMASKDKNPSHLVYEVADLWFHTMILLTHHDLKAEDVLDELARRQGLSGLAEKA
                     ARTES"
     gene            11769..12092
                     /locus_tag="WGQ68_000740"
     CDS             11769..12092
                     /locus_tag="WGQ68_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HEZ0714583.1"
                     /translation="MDNCIFCKIAAKDIPAQTVYEDGEMVCFKDINPAAPVHLLLIPK
                     VHFDSLAHAAPEHQPLLGKMMLKVPEIAKAAGLADGFKTLINTGKGGGQEVFHLHIHI
                     MGTPV"
     gene            12139..12342
                     /gene="tatA"
                     /locus_tag="WGQ68_000741"
     CDS             12139..12342
                     /gene="tatA"
                     /locus_tag="WGQ68_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HEZ0714584.1"
                     /translation="MGSFSLTHWIIVLIIVVLIFGTKKLRNVGKDLGGAVHDFKQGLN
                     EGTDGKEAQKDDVIEHKKDEDKA"
     gene            12346..13032
                     /gene="tatB"
                     /locus_tag="WGQ68_000742"
     CDS             12346..13032
                     /gene="tatB"
                     /locus_tag="WGQ68_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatB"
                     /protein_id="HEZ0714585.1"
                     /translation="MFDFGLGELVFVGIIALIVLGPERLPEAARTAGRLIGRLQRFVG
                     SVKQEFDTQIELEELRKAKQEFEAAAAQVRDSLKEIGTDMEGNLHDISNGLKPWEKLP
                     EQRTPADFGVDENGNPLPDAANTLSDGISDVMPSERSYASAETLGDSGQTGSTAEPAE
                     TDQDRAWREYLTASAAAPVVQTVEVSYIDTAVETPVPHTTSLRKQAISRKRGLRPKHR
                     AKPKLRVRKS"
     gene            13047..13817
                     /gene="tatC"
                     /locus_tag="WGQ68_000743"
     CDS             13047..13817
                     /gene="tatC"
                     /locus_tag="WGQ68_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEZ0714586.1"
                     /translation="MSETQNEQPVQPLVEHLIELRRRLMWTVVGILVCFFGMMPFAQQ
                     LYTFIANPLMANLPKDTSMIATDVIAPFFVPVKVTLMAAFLISLPHTLYQIWAFVAPA
                     LYQNEKRLITPLVLSSVSLFFIGMAFAYFLVFPVIFKFLAGVTPVGVNMATDIDKYLS
                     FILGMFVAFGTTFEVPIVVILLTKIGVVTTGQLKRARPYVIVGAFVIAAIITPPDVIS
                     QTLLAIPLILLYEAGIWFGRFFTPRSEQDGDIRPPATT"
     gene            13870..14478
                     /gene="maf"
                     /locus_tag="WGQ68_000744"
     CDS             13870..14478
                     /gene="maf"
                     /locus_tag="WGQ68_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEZ0714587.1"
                     /translation="MNTLYLGSNSPRRMEILTQLGYQVVKLPADINETVRQNEDPARY
                     VQRMAEEKNRTALTLFCETNGTMPDFPLITADTCVVSDGIILGKPRSQAEAIEFLNRL
                     SGKQHTVLTAVCIHYRGKTSSRVQTNRVVFKPLSSEEISAYVQSGEPMDKAGAYAVQG
                     IGGIFIQSIGGSFSGIMGLPVYETVSMLQDLGYRSPLSALKP"
     gene            14487..14840
                     /locus_tag="WGQ68_000745"
     CDS             14487..14840
                     /locus_tag="WGQ68_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HEZ0714588.1"
                     /translation="MNRQTAYLLTSFSLIALIILSLSWELWIAPLRPGGSWLALKALP
                     LCLPLSGILKKKIYTYQYSSMLVLIYFAEAVMRLFDAYPAEQICAALSAVFSIIFFIS
                     CLSFVKQYKETNNAR"
     gene            14830..16644
                     /locus_tag="WGQ68_000746"
     CDS             14830..16644
                     /locus_tag="WGQ68_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase C-terminal domain-containing
                     protein"
                     /protein_id="HEZ0714589.1"
                     /translation="MPAETTVSGAHPAAKLPIYILPCFLWIGIVPFTFALRQQPSPDF
                     YHDAAAAAGLIVLLFLTAGKKLFDVKIPAISFLLFAMAAFWYLQARLMNLIYPGMNDI
                     VSWIFILLAVSAWTCRSLVAHFGQERIVTLLAWSLLIGSLLQSCIVVIQFAGWEDTPL
                     LQNIIVYSGQGVIGHIGQRNNLGHYLMWGILAAAYLNGQRKIPAALGTICLIMQTAVL
                     GLVNSRTILTYIAAIALILPFWYFRSDKSNRRTMLGIAAAVFLTALFQFSMNTILETF
                     TGIRYETAVERVANGGFTDLPRQIEWNKALAAFQSAPIFGHGWNSFAQQTFLINAEQH
                     TIHDNLLSNLFTHSHNIVLQLLAEMGISGTLLVAATLLTGIAGLLKRPLTPASLFLIC
                     TLAVSMCHSMLEYPLWYVYFLIPFGLMLFLSPAEASDGIAFKKAANLGILTASAAIFA
                     GLLHLDWTYTRLVNAFSPATDDSAKTLNRKINELRYISANSPMLSFYADFSLVNFALP
                     EYPETQTWAEEATLKSLKYRPHSATYRIALYLMRQGKVAEAKQWMRATQSYYPYLMPR
                     YADEIRKLPVWAPLLPELLKDCKAFAAAPGHPEAKPCK"
     gene            16834..17511
                     /locus_tag="WGQ68_000747"
     CDS             16834..17511
                     /locus_tag="WGQ68_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEZ0714590.1"
                     /translation="MSRVLLVDDDALLTELLTEYLSAEGLNVRSVPDGEAGVQEILSG
                     QYDVVVLDSMMPKMNGLDVLKNVRARSTVPIIMLTAKGDDIDRIIGLEMGADDYVPKP
                     CTPRELLARINAILRRAQHSGEQNNAPNSISVSDVVLYPAKRQASVKDMPLELTSTEF
                     NLLEVLMRHAGQVVSKETLSVEALDRKLAKFDRSIDVHISSIRHKLGDASLIQTVRGL
                     GYLFVKN"
     gene            17525..18931
                     /locus_tag="WGQ68_000748"
     CDS             17525..18931
                     /locus_tag="WGQ68_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEZ0714591.1"
                     /translation="MKLFQRIFATFCAVIVCAIFVASFSFWLVQNTLAENQFNQRRTI
                     ETTLMGSIISAFRARGDAGAREILTEWKDSPVSSGVYVIQGDEKKDILNRYIDSYTIE
                     RARLFAAGHPHSNLVHIEYDRFGEEYLFFTKDWDKLQARRLPSPLLIPGLPLAPIWHE
                     LIILSFIIVVGLLMAYILAGNIAKPIRILGNGMDRVANGELETRISQQVDDRDDELSH
                     LAIQFDKMVEKLEKLVAKERHLLHHVSHEMRSPLARMQAIVGLIQAQPQKQEQYLKRL
                     EGELTRMDTLAGELLTLSRLETSNMALEKESLKLLPFLGNLVEDNQSIAQKNGQTVTL
                     SADGKIPENTTILANESYLYRAFDNVIRNAVNYSPEGSTILINIGQDHKHWIIDVTDN
                     GPGVDEMQLPHIFTAFYRADSSANKPGTGLGLALTQHIIEQHCGKIIAENIKPNGLRM
                     RFILPKKKTGSKTEKSAN"
     gene            19049..21475
                     /locus_tag="WGQ68_000749"
     CDS             19049..21475
                     /locus_tag="WGQ68_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HEZ0714592.1"
                     /translation="MSAQTDPGYFFMPNHIILIGASERPYSLGERVLSNLLSTPFQGK
                     ITPVNPRHHTIAGLPAYTSLNKIPGSADLIIAVTPPDSYDTLFKTCRKKQLRHIILIQ
                     DWDSLSAAELHTAETAIRKHHGNGLNITACTTAGIQLPSLGLNISTQDGYAAGHTAIL
                     TGNAAVSRQIDNILNKLRQGTSRHISLNYGISPITSSDWLNRFGHSLHTKTAVLHHNP
                     EEDQRKLFSAIRQFTRHTPLILHITCRTTETDRAVLHCLARHCNFLISFNADDLEAAL
                     RAQLSDLPPLSRLDILSDTPAEWLNAHAPKTLALHFPNLPHHIRNGHLTGTPTPSICH
                     DIASRQLARPDTQAVLTILSPSGHEDYKKTARALIRLSEQTAKPLLVSSPFSDGITHF
                     DTPTQAIRTLSYRNTAAALKQAQLDIAPPQPCRLKTPQPQNIKKALEAADPALIAEAL
                     HLPPYRHTTHNAVQFQFGSHPLYGDILTARCNGQTTAVLPPFTTLDSRHLARFAELDG
                     TQTLDQFLHTLTIIPEYRQHILGITLNLNGGQYSSDFILKTPETHDTPKRKNTGKAAQ
                     TLEHAAAKMQSAAAYLKHKNPTAAEFLRHTSEAAAELLGSKTETGAAVPNVLAPYPAA
                     HLKTLSLKNNTTVTITPLLPEDAEAKQQFVRSLSPEARYTRFMTHTNELPAATLARLC
                     NPDYHCEAAWTAKDADSNIVAVVRHSRLNRNECEFGITLAEHMRGSGLAQKMMELIIQ
                     TAAQQGYRTMSADILKTNTPMIKLAEKSGFTLKESDTEKNLYRAYLNLAADKTTEKTN
                     KNLHTDHKIT"
     gene            21520..21765
                     /gene="rpsP"
                     /locus_tag="WGQ68_000750"
     CDS             21520..21765
                     /gene="rpsP"
                     /locus_tag="WGQ68_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEZ0714593.1"
                     /translation="MVVIRLARGGSKHRPFYNVIVTDSRSRRDGRFIERVGFYNPVAN
                     EKQERVRLNADRLNHWIAQGAQVSDSVAKLIKEQKAA"
     gene            21781..22290
                     /gene="rimM"
                     /locus_tag="WGQ68_000751"
     CDS             21781..22290
                     /gene="rimM"
                     /locus_tag="WGQ68_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEZ0714594.1"
                     /translation="MTDTQNRVAMGYIKGVFGIKGWLKIAANTEYSDSLLDYPEWHLV
                     KDGKTISITLEAGKVVNGELQVKFEGINDRDLAFSLRGYTIEIPREAFAPTEEDEYYW
                     ADLVGMTVVNKDHTVLGKVSNLMETGANDVLMIDGEHGQILIPFVSQYIETVDTGSKT
                     ITADWGLDY"
     gene            22290..23039
                     /gene="trmD"
                     /locus_tag="WGQ68_000752"
     CDS             22290..23039
                     /gene="trmD"
                     /locus_tag="WGQ68_000752"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEZ0714595.1"
                     /translation="MLIQAVTIFPEMFDSITRYGVTGRANRQGIWQFEAVNPRKFADN
                     RLGYIDDRPFGGGPGMIMMAPPLHAAIEHAKAQSSQTAKVIYLSPQGKPLTHQKAAEL
                     AELPHLILLCGRYEGIDERLLQTSVDEEISIGDFVVSGGELPAMMLMDAVLRLVPGVL
                     GDMQSAEQDSFSSGILDCPHYTKPLEFQGMAVPEVLRSGNHGLIAEWRLEQSLRRTLE
                     RRPDLLEKRSLIPKESRLLETIRQEQREIQS"
     gene            23055..23420
                     /gene="rplS"
                     /locus_tag="WGQ68_000753"
     CDS             23055..23420
                     /gene="rplS"
                     /locus_tag="WGQ68_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEZ0714596.1"
                     /translation="MNLIQQLEQEEIVRLNKEIPEFAPGDTVVVSVRVVEGTRSRLQA
                     YEGVVIARRNRGLNSNFIVRKISSGEGVERTFQLYSPTVEKIEVKRRGDVRRAKLYYL
                     RGLTGKAARIKEKLPARKG"
     gene            23742..24467
                     /locus_tag="WGQ68_000754"
     CDS             23742..24467
                     /locus_tag="WGQ68_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010361246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714597.1"
                     /translation="MSIIVENLTVSYRRRPAVHHVDITFEEHSMWAVFGPNGAGKSTF
                     LKSLMGLQPIDTGSIRLDGLSRQDIAYLPQQSDIDRSQPMTVFDLAAMGLWYEIGFFK
                     GINTAQKQRVHEALERVGMRQFARRQIAHLSNGQFQRVLFARMLVQNAKFLLLDEPFN
                     AVDARTTYELLDVLQKCHCGGHAIIAVLHDYEQVRAYFPSTLLLAREKIAAGATETIL
                     TEPYLAQANAKMQQQESPDWCAS"
     gene            24502..25377
                     /locus_tag="WGQ68_000755"
     CDS             24502..25377
                     /locus_tag="WGQ68_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEZ0714598.1"
                     /translation="MNLYDLLLAPFAEFDFMRYALASVFCLSLSAAPVGVFLVMRRMS
                     LIGDALSHAVLPGAAVGYMFAGLSLPAMGLGGVAAGMLMALLAGLVSRFTTLKEDANF
                     AAFYLSSLAIGVVLVSKNGSSVDLLHLLFGSVLAVDIPALQLIAAVSSLTLITLAVIY
                     RPLVLESIDPLFLKSVGGKGGLWHVLFLVLVVMNLVSGFQALGTLMSVGLMMLPAITA
                     RLWAKHMGALILLSVLTALLCGLSGLLISYHIEIPSGPAIILCCSVLYLFSVILGKEG
                     GILTKWLKNHRHHTT"
     gene            25454..26368
                     /locus_tag="WGQ68_000756"
     CDS             25454..26368
                     /locus_tag="WGQ68_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEZ0714599.1"
                     /translation="MKHLKLTLIAALLTTAATAAPLPVVTSFSILGDVAKQIGGERVS
                     IQSLVGANQDTHAYHMTSSDIKKIRSAKLVLLNGLGLEAADVQRAVKQSKVSYAEATK
                     GIQPLKAEEEGGHHHDHDHDHEGHHHDHGEFDPHVWNDPVLMSAYAQNVAEALIKADP
                     EGKVYYQQRLGNYQMQLKKLHSDAQAAFNAVPAAKRKVLTGHDAFSYMGKRYHIEFIA
                     PQGVSSEAEPSAKQVAAIIRQIKREGIKAVFTENIKDTRMVDRIAKETGVNVSGKLYS
                     DALGNAPADTYIGMYRHNIKALINAMKQ"
BASE COUNT         6868 a         7928 c         6110 g         5604 t
ORIGIN      
        1 tgcctgcgac gcttaacggc tgtctttcca ctgataatat ttcgagccga ggaaagaacc
       61 gagtttgcgc aaaaacggtt gcaggataat attcggctgg cgcaaccgct caaacggctt
      121 cagacggcat tcgtccccta aaattgcttc ggcaaccgcc agacctgcaa tgcctgttat
      181 cgccatcccg tgtccggaat aaccttgcgt ataaaaaaca ttcggggcta aacgtccgaa
      241 atgcgggaca aggttggcgg taatgtcgca ctccccgccc cacgaatatt cgattttgac
      301 atcggcaagc tgcggaaaaa ctttaagcat atcttggcgg acaagctcgg tcatacgcgc
      361 gggattgtcg ataaactcgt tatccttacc gccgaaaagc agtctgccgt ccgcgctgag
      421 gcggtaataa tccaaaatat ggcggttgtc gcatactgcc atattgttgc ggataagccc
      481 ttttgcacgc gcgcccaagg gttctgtggc aataataaag gtgctgacag caatcgcctt
      541 gcgctccaaa ggcttgaata tcggattcaa accggcataa gtattgacgg cgtacaccag
      601 atttttgcat tcgacgctgc cttcgggggt gtaaaccagc caaccgtttt gataaggttc
      661 aatgcgtatc atgggagaat gctcaaaaat cttcgtacca gcttcggcag cggcgcgggc
      721 gatgcccaac gtgtaattga gcggatggag atgcccggac aagggatcga actgtgcgcc
      781 ttggtacata tcgctgtcaa gctgctgctt cagttcagtg ttatcccaga gttgataatg
      841 agttgcaccg taatattttt gggcgtgctc atgccattgt tgcaattctt cccaatgctg
      901 cgaacggatg gcaaccgtgg cataaccgcg ctgccaatcg caatcaatgg catgtttgcg
      961 gacgcgttcg tccaccagtt cgaccgcctg caaagactgt tgccaaaacc attgcgcttg
     1021 ctccaaaccg acctgttttt caatttcctc cataccgcag gcgtaatcgc tgataacctg
     1081 cccgccactc cgtcccgacg cgccgaaacc gatacgcgcg gcttccaaca caaccgtttc
     1141 atgtccctgc tccgccaagg gcaatgcagt gcacaaacca ctcaatccgc cgccgatgat
     1201 acaggtatcg gttttcagac ggcattgaag tttcggataa acagtatgag gattaaccga
     1261 actgaaataa taagaaggca gatattcttg aaaatcaggg cgaatcattg tgtttgcttt
     1321 atcgggtata ttttcggacg gaatgataca ggctgtcgag ccatatcgtc caaacagaaa
     1381 atcggttgaa gaaaacaggc tgacccagtc atgcggtcag cctgccttat taattaattt
     1441 gctttctcgg cagccaattt ttcttggcgg taggcttctg ccgcttctcg gtcacgcttg
     1501 gttgcctgcc tcatcatcca ataattgacg atgatgacca atgttccgat gatgccgatc
     1561 aggatggttg ccaaaacgtt catctgcgga tcaagaccca gcttgatttt ggagaaaatc
     1621 acctgcggca atgtggatga accggggccg gagaggaatg aggtaatcac caaatcatcc
     1681 aaagacaggg taatgccgag cagaaagcct gaagcgatgg caggggcaat caaaggcaaa
     1741 gtgatgacaa aaaagatttt cagcggacgc gcgcccaaat ccattgcggc ttcttcgagc
     1801 gattggtcaa gctcaaccag acgcgaacgg ataacaacgg taatgtatgc catgcacagc
     1861 gtcgtatgtc ctaagaaaat agtgaaaaag ccacgatcga agtagagatg ttgtaaccat
     1921 tcgctgccct gcaaaaatat ctgtacctga ataatcagca gcagcataga cagaccggta
     1981 atcacgtcgg gcatcaccat aggtgcggaa atcataccgg cgaacaaggt actgccgcga
     2041 aaacgtttaa tccgcgccat cgcatagccc gccagcatgc ccaaaacgac ggcggcaagc
     2101 gaagacacaa cggcaatccg cagcgacagc caagcggctt ccaaaatggt gtcgttttcc
     2161 aacaatgcgc cgtaccactt ggtcgaaaag ccgccccaaa cggttaccag cttggattcg
     2221 ttaaacgaat agatgaccaa aacgaccagc gggatataca gaaacgccag cgacagtgcc
     2281 aacatcagtt tcaagaacca agataatttg gatttctgca ttatttggct ccttcttcca
     2341 attcgcggtt ttcataatgc tgaaacaggg caatcggcac gaccagcagc gcgaccatca
     2401 cgacggcgac ggcggaagcc agcggccagt tgttttgatc gaagaacgcc tgccacaaga
     2461 ctttaccaat catcaggttt tccgaaccgc cgaccagctc gggaatgacg aactcgccga
     2521 cagcagggac gaaaaccagc atggagcctg caataatgcc ggttttcgac aaaggcaggg
     2581 taatcgtcaa gaacgatttg accggccccg cgcccaaatc ggaagccgct tcaagcaggc
     2641 ggttgtcgag tttcaccagt tgcgtgtata gcggcagaat cataaacggc agataggcgt
     2701 aaaccatcac caaattgagc gaaaaggcat tgtagaacaa atccaaaggc tcgctgataa
     2761 tacccatttt aatcaacagg ttgtttacaa taccgttatg cccgagcaga cccatccacg
     2821 catagacgcg caacaggaac gatgtccaaa agggcagcat aatggcaagc agcaaaccat
     2881 tgcggacaga aggattggca cgagaaatcg cataggcggt cggataaccg accaacaggc
     2941 aaatcactgt cgtcatcaat gcggttttga ttgaagacca gtaggtcatc agatagatat
     3001 tgctgttttc actgtcgccg aacgggttaa gggtatgcca aaaattttgg aaaatatcgg
     3061 catagttttg ataactgatg gcgatattca gacgacctaa atcttcgtcg atgctggtca
     3121 gcggcgtaaa cggcggaatg gcgatttctt gttcggcaaa gctgattttc agcacgatgg
     3181 cgaacggaat cagaaacagc accaaaagcc aaatatacgg tacggcaatc accacacgct
     3241 gccccgggcg gcggaacagt ttgtttttca gtttattaag gttcattgca ttccccttaa
     3301 atcaacggaa caacggagtc ggttggtttt ccggccagct gatatagacg gtttcgtccc
     3361 aagttggcgg tgtaatgttg cgcacatacc agtaaggggc ggggacttgg cttttgacga
     3421 cgcgcccgtt tgcgagtttg atatggtata tggcgaagct gcccaaatag gcaatttctt
     3481 ttaccgtgcc tttcgcccag ttgtagtcgc ccaaatattc gggtttttct ttatataaat
     3541 caatatcctc tggtcgaatg ctaacccaaa ggtcctgctc gctcggacca cccaaaccgt
     3601 gatcgatgcg gacgtggttt tccaaacctt cgcattcgat aacggcatag tcggcatgat
     3661 cttcaatcac cacaccgtca aagatgttgg tttcgccgat aaactcggca gtgaagcggc
     3721 tgttgggata gtcgtacacg tcgctgggtg tgccgacttg ctgcaactga ccgtcggaca
     3781 taatggcgat gcgggtcgcc atcgtcatcg cctcttcttg gtcgtgtgta accataatac
     3841 aggttacgcc gacttgttcc agcgtattga ccaactcaag ctgggtttgt tggcgcagtt
     3901 ttttgtccaa tgcaccgagg ggctcatcca gcagtagaat tttcggacgt tttgccagac
     3961 tgcgtgccaa agcaatgcgc tgctgctgac cgccggacaa ttggtgcggt ttgcgtttag
     4021 caaatttggt catctgaacc aggcggagca tttcttcgac gcgcgcggcg atttcgcctt
     4081 taggcatttt gtcctgtttc agaccgaagg caatgttttg ttctacggtc atatgcggaa
     4141 aaagcgcgta actttggaac atcatattga tggggcgatc atagggtgca agtttggtaa
     4201 tatcctgacc atcaaggata atttttccct gattgggact ttccataccc gccagcatac
     4261 gcagcagtgt agattttccg ctgccggaac tgcccaaaag ggcgaagatt tcgtgttgat
     4321 aaatgtccaa gtcgatgtta tcgacagcgt aattgtcacc aaactttttc accaaacctt
     4381 ggattttgag ataaggtttg gctgaagacg cagtggttgc ggtcataatg gcaatactcc
     4441 aataaaaaga cgagtaccgg caaaacggat tttcgaatgg gtgataaaaa gctgtttgat
     4501 tgctggcggg agttaaacgt ttgatgccgt ctgaaactct tgtaaagcgc acgggcagca
     4561 tgaaatggaa caagattcca aagaacttta tattatatta gtttatgcgg ttttcgggca
     4621 atatagtgga ttaaatttaa accagtacag cgttgccttg ccttgccgta ctatttgtac
     4681 tgtctgcggc ttcgtcgcct tgtcctgatt taaatttaat ccactataaa tatggatttg
     4741 agcttgtcgg aaagcaacag aaaagaaaac accgcccatt tttctgggcg gtgtcggaaa
     4801 gcgtaattat ttacgcagac ccaagcgggt aatcaacgcg cgatacgtat cgggctgggt
     4861 acggcgcaag taggccagca ggcggcggcg ttggctgacc attttcaaca ggccgcgacg
     4921 gctgtggtgg tctttggggt tggctttgaa gtggggggtc aggtcgttga tgcggaaagt
     4981 caacagagcg acttgtactt cggaagagcc ggtgtcgcct tctttgcgtt ggaaatcttt
     5041 aacgatttgt gctttttgtt ctacggtcag tgccataatg aaaactccaa aaatataaga
     5101 atccccatag ggattcagac aagtttgcca agcctgaaga caacggcaaa ctccctatgc
     5161 ccaagatagg acaacgtggc attatgacac aattcccgca tttctgcaca atattttaag
     5221 accgtggaag taaccgttaa aaatgccgtc tgaagcattg tctgcttgag acggcaatgt
     5281 tcagacggca tatgcgcttc aaaccacgat ttcttctttc ttcttcggtt ctttaccgat
     5341 attgtcgcgg ctcaaaccga acattagcag aagcgggctg gcaaccaata cggaagaata
     5401 aatgccgaac acgatgccaa tggtcaacgc catagaaaag ccgtgcaagg ccgcaccgcc
     5461 gaacaccagc atagaaacaa ccatcgcctc ggtcgaaccg tgggtaatga tggtgcggct
     5521 catcgttgcg gtaatcgcgt tgtcgatgac ttccggcacg gcatgtccgc gcatcgccgg
     5581 cttgcggaag ttttcacgga tacggtcgaa gacgacgacg gattcgttca cagaatagcc
     5641 caatacggca aggatacccg ccaagacggt cagcgaaaat tcccattgga agaaggcaaa
     5701 gcagccgaga ataatcacga tgtcgtgcat attggcgata atggcagata cggcaaaacg
     5761 ccattcaaaa cgcatcgaca ggtaaataat gatgccgata acgacaaaac ctaaagccat
     5821 caatccatta cttaccaatt cctcaccgac ttgcgggccg ataaattcga cttggcgcaa
     5881 ggtaacgtcg ggactgtctt ttttcagcaa atccataacc tgattggaca actgtgcgga
     5941 agtaacacct tctttgttcg gcaggcggat catgatgtgt ttgttcgtac ccaatgcctg
     6001 aacctgtaca tcacctattt tcagcgtatc gaggcgttcg cgcatcttat tgacatccgc
     6061 accctgctga tattggactt ccattaccgt gccgccggta aattcgacag agaaattcag
     6121 acctctggta accaaaaaga atacggcggc aataaaggta accaacgaaa taaaggtcgt
     6181 cagtttgccg tagctcataa acggaatatc gcgtttgatt ttaaagagtt ccatagctta
     6241 ctccttgcct cctgccattt cagctttcgg cttccacacc gaaccgatgg aaatattctg
     6301 caatttgcgt ctgcgtccgt accacagatt gaccaacgca cggaatacga cgacggacga
     6361 atacatcgaa gtcagaatac ccaaacagtg tacgaccgca aaaccgcgta ccgggccgga
     6421 accgaatacc aaaagcgcga taccggcaat cagcgaagtc aggttggaat cgacaatggt
     6481 cgcccatgcg tgttggaaac cgagattgat tgcctgctgc ggcggcacgc cggcacgcaa
     6541 ttcttcgcgg atacgttcgt taatcaagac gttggagtcg attgccatac ccaaagtcaa
     6601 cgccagcgcg gccatacccg gtaacgtcaa cgttgcctgc atggcagaca aaataccgat
     6661 taggaacagt atgttggcac tcaatgcaat ggtagaaaag aaacccatca gacgatagta
     6721 aaccaccatg aatgcagcaa cgatggcaaa accccataaa gtcgaatgga agcctttttc
     6781 gatgttctcc ttacccaaag acggaccgat ggtacgttct tcgacaatct gcatcggtgc
     6841 ggcaagagaa ccggcacgca acagcaaaga cgtatcattg gcttcggctg tcgtcatgct
     6901 tccggaaatt tccacgcgtc cgcctgtaat ggcagtacgg ataaccggcg cggttacaac
     6961 ctcggatttt ccttggtcga tcaaaaccat cgccatgcgt ttgccgacat ttgcggcagt
     7021 cagttcgccg aaaatgctgc cgcccgcgct gtccaagctc agactgacgg caggtgcgcc
     7081 catttggtcg aaactcggtt gcgcatcgtt gatgttgtcg cccgtcagct cgacctgttt
     7141 gctgattagc agagtttcgg gacgatctcc gccgcttgaa agcagctcat aaccgctcgg
     7201 cacgttgcct tccaatgcct cgcgcaactt ggcaggatcg tcctccacca tacgcaattc
     7261 caaagtcgcg gtacggccga tgatgtcttt tgccttggca gtatcctgaa cgcccggaag
     7321 ctgcacgacg atacggtcgg cacctgactg ctggatgacg ggttcggcca cgcccaactc
     7381 gttcacacgg ttgtgcaggg tagtgatgtt ctgtttgacc gcatcggaac gcactttatt
     7441 gaccgcctct tccgaaagcg tcaagacgat attgctgccg tctgaattca gcgttgcttc
     7501 aggaaacagc ttgcgcaact gcggcagtgc cttttgcaca tcacctgcat cctgcaaagg
     7561 gacggtcagg ctgtttccag cctgacgcac cgtgccgctg cggatttttt cgcggcgcag
     7621 ttcgcggcgg atgtcgcccg aataacgttc aaacgttttc tgcatcgccg ctttcatatc
     7681 gacctgcatg gtgaaatgca cgccgccgcg caggtccaaa cccaaaaaca tcggattggc
     7741 tttgattttc gccatccatt cggggctgtc cgccaacagg ttgagcgcgg taatataccc
     7801 ttcgcccaaa gtgttttcga tgacgtcgcg cgctttaagc tgcgtttctg tgtctttgaa
     7861 acgcactttc agtgaattgt ccacaacaaa catcccgtcg gtctgaatac ccgcgttttg
     7921 cagcgcggca tccactttgg attgagtctg ttcgttgatg atgatggctt gtcggttggt
     7981 cgatacctgc acggcgggtg tttcgccgaa taggttgggc agcgaataca ctgcggcaac
     8041 cgcaatcgtg aacacaatca gcagatattt ccataaagga taacggttca tcattgttcc
     8101 ttaatggttg gaaccccacc ctttcggtgg tgtcggaatc gggctatttc agaagaggca
     8161 aaaacccttc ccagccaggc aagaccggaa ggcggcatct tgaatatgcc gccctgcgtg
     8221 tcggaacatg gtcaagcctt cggttggaat tcaaaacaaa gtgccgcatt cgggctttcc
     8281 agatgcggct tgtcggcaca aatcaatcga cttttgcggc aatcgcattg cgttctactt
     8341 cgacctcgat ttttgtaccc tgtccgatat ccacggtaaa aaactgttcg ccgactctgg
     8401 ttaccttacc cttgaaacct gccgccaaga ccactttgtc gccgactttc aaggcggcaa
     8461 gcattgcctg atgcgctttg aatttctttt gctgcggacg catgatcagg aagtagaaca
     8521 ccaccataat caacactaaa ggagcaaatt gtgcaacagc ttgattcata atttatccgt
     8581 tctttctaat atggttgaaa atcgagaggg gtatataata acataagacc gtaaacaata
     8641 tatcgggttt gccgtccgta ccgaccgtat accgcagcct gcccgtccac aaacccatgt
     8701 ccttgacccg cctaatcttg aaattctatg cgctgttgcg cctctttttg ggcaaaaacg
     8761 cccgcaccgc atggatttcg catcccgcct gcaccgggca cgaacccggc gcaaaccatc
     8821 ccgattcgcc cgaccgcatc ctctgcatcg agcaggcatt gcgccgcgcc ggtatttggc
     8881 agcacctcca aaccatagag gcggaagaaa tcagcgatac gcgcctcgcg cttgcccata
     8941 cgagcaaata tttaaaccgg ttagagtccc gcctgcccga agacggcaag atttgccgct
     9001 tggacgacga caccgttatc agcaaaaact ccctgtctgc cgcccgtttt tccgcaggtt
     9061 cggcagttca ggcagtcgac atggtcatga accgtaaagc atggcatgcc ttttgcgccg
     9121 cccgcccgcc cggacaccat gcgggcagcg gcaaagccgg cggattctgc ctgctgaaca
     9181 acgttgccgc cggtgcgatg tacgcccttg ccgaataccg gctgaaccgc atcgccgtca
     9241 tcgattttga tgtacactac ggcgacggta cggcagaaat attcaaagac gatccgcgca
     9301 tcctgttttt caacctgttt gaaaccgacc ttttcccctt ccccgaaaac aacgatatgc
     9361 ccgacggcgg caatatggtg cacctgccct tgccgccggg aacgggcagc cgcacattcc
     9421 gcgaagccgt ccgcaggcag tggctgcccc gacttgccgc attcaaaccc gaactggtgc
     9481 tgctgtcggc aggattcgac gcacaccgtc tagacgaatc gggcaggctc aacctgcacg
     9541 aggcggattt tgcctggctg acacacaaaa tcattcagac ggcatcgggc tgccccggca
     9601 aaatcgtatc cgtactcgaa ggcggctaca cccttgaacc tctggcacaa tctgctgccg
     9661 agcatatccg cgtgctggcg gggctgggca aatccgatgc cgcaaccgcc tatcagaaaa
     9721 cactggatcc aacaaaaaaa cggtttgcca aaccgaaaac cggacaggtg cgccaaccta
     9781 cccaatccga ccgttacgac atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     9841 cgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcaca ttagagaatc
     9901 gttctctttg agctaaggcg aggcaacacc gtactggttt ttgttcatcc accataaatg
     9961 ccatctgaag ccgattcggt tttcagacga catcattatt tgacctgacg caaacagaaa
    10021 gccttccgcg tgcttaaaca catctcttgt agtaaaatat ctaaacagat tcaactggaa
    10081 acggtcaggc ttcttcacgc cattcccatt tcaaccgccg cctgccagat tcagacggta
    10141 ttcctacttg aaaacaagga gcgttacaat gaaagcagca cgtttttacg acaaaggcga
    10201 catccgcatc gaagacatcc ccgaaccgac cgtcgccccc ggcaccgtcg gcatcaatgt
    10261 tgcctggtgc ggcatctgcg gtaccgacct gcacgaattc atggaaggcc cgattttcat
    10321 tccgccttgc ggtcatccgc acccgatttc cggcgagtcc gcacccgtca cgatgggaca
    10381 cgagttctcc ggcgtggtct atgccgtcgg cgaaggcgtg gacgacatca aagtcggtca
    10441 acacgtcgtg gtcgagcctt acatcgtaca cgacgacgta ccgaccggct cgggcagcaa
    10501 ctaccacctc tccaaagaca tgaactttat cggcttgggc ggctgcggcg gcggcctgtc
    10561 cgaaaaaatc gccgtcaaac gccgttgggt gcatccgatt tccgacaaaa tcccgttgga
    10621 tcaagccgct ttgatcgaac cgctgtctgt cggacaccac gcctatgtac gcagcggcgc
    10681 gaaagcaggc gacgtcgcat tggtcggcgg tgcaggtccg atcggtttgc tgttggctgc
    10741 cgtgttgaaa gccaaaggca tcaaagtcat catcaccgag ttgagtaaag cacgcaaaga
    10801 caaagcgcgc gaatctggcg ttgccgacta catcctcgac ccgtccgaag tcgatgttgt
    10861 cgaagaagtg aaaaaactga ccaacggcga aggcgtggac gtggcattcg aatgtaccag
    10921 cgtgaacaaa gtgttggata ctttggtcga agcctgcaaa cctgccgcca atttggttat
    10981 cgtatccatc tggagccacc ctgcaaccgt caacgtccac agcgtcgtga tgaaagagct
    11041 ggatgtgcgc ggcacgattg cctactgtaa cgaccacgcc gaaaccatca aactggtcga
    11101 agaaggcaaa atcaacctcg aacccttcat cacccagcgc atcaagctgg acgaactggt
    11161 ttccgaaggc ttcgagcgtc tgatccacaa caacgaatcc gccgttaaaa ttattgtgag
    11221 tccaaacctg taagcaaatt acatttgcaa cagcacaaat gccgtctgaa acgctcagac
    11281 ggcattttcc aacgaagcgc aacaggcgta ttgcccgaga gcagccgttt agcctatcat
    11341 tccgaacaaa cagaaacaaa cagaaggaaa atcatgggag attccgtact atccgccatc
    11401 caacaaacca tcacccagcg aaaatctgcc aatccgtccg aatcttacgt cgcacagctc
    11461 ttgcataagg gcgaagacaa aatcctaaaa aaagtgattg aagaggcggg cgaagtgttg
    11521 atggcatcca aagacaaaaa cccgtcccac ctggtttacg aagttgccga cttatggttt
    11581 cacaccatga ttcttctgac acaccacgac ctgaaggcgg aagacgtatt ggacgaactt
    11641 gcgcgccgcc aaggtttgtc gggcttggcc gaaaaagccg ctcgcacaga atcttgaatt
    11701 tatattaaaa tccgcacttt cccacattca atccgtctga ccgctgttca gacggcaacg
    11761 gagccgttat ggacaactgt attttctgca aaatcgccgc caaagacatt ccggcgcaaa
    11821 ccgtctatga agacggcgaa atggtttgtt tcaaagacat caaccccgct gctccggttc
    11881 atctgctgct gattcccaaa gtccatttcg attcgttggc acacgccgcg cccgaacatc
    11941 agcccctttt gggaaaaatg atgctgaaag ttcccgaaat cgccaaagcg gcaggactgg
    12001 cagacggctt caaaaccctg atcaacaccg gaaaaggcgg cggacaagag gtcttccacc
    12061 tgcatataca catcatgggc acacccgtat aaaccgttat ttcacaatca acccctaata
    12121 cttacttaag gatacatcat gggcagtttt tctctgacgc actggattat cgtactgatt
    12181 atcgtcgttt tgatattcgg caccaaaaaa ctgcgcaacg tcggcaaaga cctcggcggt
    12241 gcggttcatg acttcaaaca ggggctgaac gaaggtacag acggcaaaga agcccaaaaa
    12301 gacgatgtaa tcgaacacaa aaaagacgaa gacaaagcgt aatttatgtt tgatttcggt
    12361 ttgggcgagc tggtttttgt cggcattatc gccctgattg tcctcggccc cgaacgcctg
    12421 cccgaggccg cccgcaccgc cggacggctc atcggcaggc tgcaacgctt tgtcggcagc
    12481 gtcaaacagg aatttgacac tcaaatcgaa ctggaagaac tgaggaaggc aaagcaggaa
    12541 tttgaagctg ccgccgctca ggttcgagac agcctcaaag aaatcggtac ggatatggag
    12601 ggtaatctgc acgacatttc caatggtctg aagccttggg aaaaactgcc cgaacagcgc
    12661 acgcctgccg atttcggtgt cgatgaaaac ggcaatcccc ttcccgatgc ggcaaacacc
    12721 ctatcagacg gcatttccga cgttatgccg tccgaacgtt cctacgcttc cgccgaaacc
    12781 cttggggaca gcgggcaaac cggcagtaca gccgaacccg cggaaaccga ccaagaccgc
    12841 gcatggcggg aatacctgac tgcttctgcc gccgcacccg tcgtacagac cgtcgaagtc
    12901 agctatatcg atactgctgt tgaaacgcct gttccgcaca ccacttccct gcgcaaacag
    12961 gcaataagcc gcaaacgcgg tttgcgtccg aaacaccgcg ccaaacctaa attgcgcgtc
    13021 cgtaaatcat aaagagggca aatccggtgt ccgaaacaca aaacgaacaa cccgtccaac
    13081 cgcttgtaga gcatctcatc gagctgcgcc gccgcctgat gtggacggtt gtcggcatct
    13141 tagtctgctt tttcggcatg atgccgtttg cccaacaact ctatactttt atcgccaacc
    13201 cgctgatggc aaacctgccc aaagacacca gcatgattgc caccgatgtc atcgcaccgt
    13261 ttttcgtgcc ggtcaaagtt accctgatgg cggcattttt aatttcgctg ccgcatacgc
    13321 tctaccaaat ctgggcattt gtcgcccccg cactctacca aaacgaaaaa cgcctgatta
    13381 cgccgctcgt cctctccagc gtcagcctgt ttttcatcgg tatggcattt gcctactttt
    13441 tggttttccc cgtcattttc aaattccttg ccggcgttac ccctgtcggt gtcaatatgg
    13501 cgacagacat cgacaaatac ctctccttca tcttggggat gtttgttgcg ttcggcacaa
    13561 cgtttgaagt ccccattgtc gttatcctgt taaccaaaat tggtgtggta acaaccggac
    13621 agctcaaacg cgcccgcccc tatgtgattg tcggcgcgtt tgtcattgcc gccatcatca
    13681 cgccgcccga tgtgatttca caaaccctgc ttgccattcc gctgattctc ttatacgaag
    13741 caggtatttg gttcggacgc tttttcacgc cacgttcaga acaggatggc gacatacggc
    13801 cgcctgcaac aacctgacac tatgccgtcc gaacctccgc ctcataccgc aacagattaa
    13861 ggaatacctt tgaataccct ctatttaggt tcaaacagcc cgcgccgaat ggaaatcctg
    13921 acacagttgg gctatcaggt cgtcaagctg cctgccgaca tcaatgaaac ggtcagacag
    13981 aacgaagacc ctgcccgtta cgttcaaagg atggcagaag aaaaaaaccg aaccgccctg
    14041 accctctttt gcgaaaccaa cggcacaatg cccgatttcc ccctgattac cgccgacacc
    14101 tgcgtcgttt cagacggcat catattgggc aaaccccgct cccaagccga agcaatcgaa
    14161 tttttaaacc gattgtccgg caaacaacat accgtcctga ctgctgtctg cattcattat
    14221 cgcggcaaaa cgtcaagccg cgtccaaacc aaccgcgtcg ttttcaagcc cctgagttcg
    14281 gaagaaattt ccgcctatgt gcaaagcggc gaaccgatgg acaaagccgg tgcctacgcc
    14341 gtacaaggca taggcggcat ctttatccaa tctatcggag gcagcttcag cggcattatg
    14401 gggctgcccg tttatgaaac cgtttcgatg ttgcaggatt tgggataccg ctcccccttg
    14461 tccgccctta aaccgtaaag accgccgtga acagacaaac cgcttacctc cttacctctt
    14521 tcagcctgat cgcactgata atcctgtccc tttcctggga actgtggatt gcaccgttgc
    14581 gcccgggcgg ctcgtggctc gcgctcaaag ccctccccct ctgtctgccg ctttcaggca
    14641 tcttgaaaaa gaaaatctat acttaccaat acagctccat gctggttctg atttactttg
    14701 ccgaagccgt catgcgcctg ttcgacgcct atcccgcaga acagatttgc gccgcgcttt
    14761 ccgcagtatt cagcatcatc ttcttcatat cctgcctgtc gttcgtcaaa caatacaagg
    14821 aaacaaacaa tgcccgctga aacgaccgta tccggcgcgc accccgccgc caaactgccg
    14881 atttacatcc tgccctgctt cctttggata ggcatcgtcc cctttacctt cgcgctcagg
    14941 cagcaaccgt cgcccgactt ttaccacgat gctgcagccg cagccggcct gattgtcctg
    15001 ttgttcctca cggcaggaaa aaagctgttt gatgtcaaaa tccccgccat cagcttcctt
    15061 ctgtttgcaa tggcggcgtt ttggtatctt caggcacgcc tgatgaacct gatttacccc
    15121 ggtatgaacg acatcgtctc ttggattttc atcttgctcg ccgtcagcgc gtggacctgc
    15181 cggagcttgg tcgcacactt cggacaagaa cgcatcgtta ccctgcttgc ctggtcgctg
    15241 cttatcggct ccctgcttca atcctgtatc gtcgtcatcc agtttgccgg ctgggaagac
    15301 acccccctgc ttcaaaacat catcgtttac agcgggcaag gcgtaatcgg acacatcggg
    15361 cagcgcaaca acctcggaca ctacctcatg tggggcatac tcgccgccgc ctacctcaac
    15421 ggacaacgaa aaatccccgc cgccctcggc acaatctgcc tgattatgca gaccgccgtt
    15481 ttaggtttgg tcaattcgcg caccatcttg acctacatag ccgccatcgc cctcatcctt
    15541 cccttctggt atttccgttc ggacaaatcc aacaggcgga cgatgctcgg catagccgca
    15601 gccgtattcc ttaccgcgct gttccaattt tccatgaaca ccattctaga aacctttacc
    15661 ggcatccgct acgaaactgc cgtcgaacgc gttgccaacg gcggtttcac agacttgccg
    15721 cgccaaatcg aatggaataa agcccttgcc gccttccagt ccgccccgat attcgggcac
    15781 ggctggaaca gttttgccca acaaaccttc ctcatcaatg ccgaacagca caccatacac
    15841 gacaacctcc tcagcaactt gttcacccat tcccacaaca tcgtcctcca actccttgca
    15901 gaaatgggga tcagcggcac gcttctggtt gccgcaaccc tgctgacggg cattgccggg
    15961 ctgcttaaac gccccctgac ccccgcatca cttttcctaa tatgcacgct tgccgtcagc
    16021 atgtgccaca gtatgctcga atatcctttg tggtatgtct atttcctcat ccctttcgga
    16081 ctgatgctct tcctgtcccc cgcagaggct tcagacggca tcgccttcaa aaaagccgcc
    16141 aatctcggca tactgaccgc ctccgccgcc atattcgcag gattgttgca cttggactgg
    16201 acatacaccc ggctggttaa cgccttttcc cccgccactg acgacagtgc caaaaccctc
    16261 aaccggaaaa tcaacgagtt gcgctatatt tccgcaaaca gtccgatgct gtccttttat
    16321 gccgacttct ccctcgtaaa cttcgccctg ccggaatacc ccgaaaccca gacttgggcg
    16381 gaagaagcaa ccctcaaatc actaaaatac cgcccccact ccgccaccta ccgcatcgcc
    16441 ctctacctga tgcggcaagg caaagttgca gaagcaaaac aatggatgcg ggcgacacag
    16501 tcctattacc cctacctgat gccccgatac gccgacgaaa tccgcaaact gcccgtatgg
    16561 gcaccgctgc tacccgaact gctcaaagac tgcaaagcct tcgccgccgc gcccggtcat
    16621 ccggaagcaa aaccctgcaa atgacccgcg ccggcggatg cggataccgc ccgaaatgta
    16681 aagctccatg caagacattg caaaaaacag caaaccggta gggaaatacg ctatcaaaaa
    16741 acattgcagc cgtgttaaga taaaccgtca aacaatcttt tcacgccccg cccgaaacag
    16801 ggtcggggca tacccttacg aaaaggaaac accatgagcc gcgtattact cgtagatgac
    16861 gatgccctgc tgaccgaact gctgaccgaa tacctgagcg ccgaaggtct gaacgtccgc
    16921 agcgttcccg acggggaagc aggcgtacag gaaatcctga gcgggcagta cgatgtagtc
    16981 gtattggatt ccatgatgcc caaaatgaac ggcttggatg tcttgaaaaa cgtacgcgcc
    17041 cgaagcaccg tccccatcat catgctgacc gccaaaggcg acgacatcga ccgaatcatc
    17101 ggcttggaaa tgggcgcgga cgactatgtc cccaaaccct gcacaccacg cgaactcttg
    17161 gcacgcatca atgccatcct gcgccgcgca caacacagcg gcgaacagaa caacgcaccc
    17221 aacagcatct ccgtcagcga tgtcgtcctg taccccgcca aacgccaggc atccgtcaaa
    17281 gacatgccgc tcgaactgac cagcaccgaa ttcaacctgc tcgaagtcct gatgcgccat
    17341 gccggacagg tagtcagcaa agaaaccctg tccgtcgaag cactcgaccg caagctggca
    17401 aaattcgacc gcagtatcga cgtacacatc tccagcatcc gccacaagtt gggcgatgcc
    17461 tctctgattc aaaccgtacg cggcttgggc tacctgtttg tcaaaaactg aaataaacag
    17521 ataaatgaaa ctgttccaac gcatcttcgc cacattttgc gcggttatcg tctgtgcaat
    17581 ctttgtggcg agtttttctt tctggctggt gcagaacacc cttgccgaaa accagttcaa
    17641 ccaacgccgc accatcgaaa ccactttgat gggcagcatc atttccgcat tccgggcacg
    17701 cggggacgcg ggtgcgcgcg aaatcctgac ggaatggaaa gacagccccg tctcatcggg
    17761 cgtgtacgtt atacagggcg acgagaaaaa agatatcctg aaccggtata tcgacagcta
    17821 caccatcgaa cgcgcccggc ttttcgccgc cggacacccg cattccaacc tcgtccatat
    17881 cgaatacgac cgcttcggcg aagaatacct gttcttcacc aaagactggg acaaactcca
    17941 agcccgccgc ctgcccagcc ccctgttgat ccccggtctg ccgctcgccc cgatttggca
    18001 cgaactcatc atattgtcct tcatcatcgt cgtcggactg ctgatggcgt acatcctcgc
    18061 cggcaatatc gccaaaccca tcagaatctt aggcaacggc atggacaggg tggcaaacgg
    18121 agaacttgaa acccgtatct cccaacaggt cgacgaccgc gacgacgaat tgtcccatct
    18181 tgccatccaa ttcgacaaaa tggtggaaaa actcgaaaaa ctcgttgcca aagaacgcca
    18241 cctgctccat cacgtctccc atgaaatgcg ttctcccctt gcgcgcatgc aggcaattgt
    18301 cggactgatt caggcgcagc cccaaaaaca ggagcaatat ctcaaacggc tggaaggcga
    18361 actgacccgc atggatacgc tggccgggga actgttaacc ctgtcccgtc tcgaaacttc
    18421 caatatggct ttggaaaaag aaagcctgaa actcctgccc ttcctgggca acctggtaga
    18481 agacaatcaa agcattgccc agaaaaacgg acaaacggtt accctgtctg ccgacggaaa
    18541 aatccccgaa aacacaacca tccttgccaa cgaaagctac ctgtaccgcg ccttcgacaa
    18601 cgtcatccgc aacgccgtca actacagtcc cgaaggcagc accatcctga tcaacatcgg
    18661 acaagaccac aaacactgga taatcgacgt taccgacaac ggccccggcg tggacgaaat
    18721 gcagctcccg cacatcttca ccgctttcta ccgtgcagac tccagtgcca acaaacccgg
    18781 aacaggactg gggcttgcat tgacccaaca tattattgaa cagcactgcg gcaaaatcat
    18841 cgccgaaaac atcaaaccga acggtctgcg gatgcgcttt atcctaccca agaaaaaaac
    18901 cggttccaaa acagaaaaaa gtgcgaactg accataatgc cgtctgaacc ggcttcagac
    18961 ggcattgcac aaacagttac tccaaaacaa acacacagcg ggtgcctctc caaaaatcac
    19021 gcacataccg tccgcaaagg aatatatcat gtcggcacaa accgatccgg gctacttttt
    19081 catgcccaac cacattatcc tgataggcgc gagcgaacgt ccgtacagcc tgggcgaacg
    19141 cgtactcagc aacctgctga gtacgccctt tcaaggaaaa atcacccccg taaacccgcg
    19201 ccaccacacc atagccgggc ttcccgccta caccagcctc aacaaaatcc ccggcagtgc
    19261 agacctgatt attgccgtta ccccgcccga cagttacgac accctcttca aaacctgccg
    19321 caaaaagcag ctccgacaca tcatcctcat acaggactgg gacagcctat ctgccgcaga
    19381 actgcacacc gccgaaactg ccatccgcaa acaccacggc aacggactca acatcaccgc
    19441 ctgcaccacc gcaggcatcc aactgccctc actcggactt aacatcagta cccaagacgg
    19501 atacgccgca ggccataccg ccatactgac cggcaatgcc gccgtcagcc gccaaatcga
    19561 caacatcctg aacaaactcc gtcaaggcac atcccgccac atcagcctga actacggcat
    19621 cagccccatc acttcctccg actggctcaa ccgcttcggc cacagcctgc acaccaaaac
    19681 cgccgtccta caccacaacc ctgaagagga tcagcgcaaa ctgttcagcg caatccgcca
    19741 atttacccgc catacgccgc tgattctcca catcacctgc cgcacgacag aaaccgaccg
    19801 tgccgtactg cactgcctcg cccgacactg caacttcctc atcagtttca acgccgacga
    19861 cctcgaagcc gcactgcgcg cccaactgtc cgaccttcca cccctgtccc gactcgacat
    19921 cctgtccgac acgcctgccg aatggctgaa cgcgcacgcg ccaaaaaccc tcgccctcca
    19981 ctttcccaac cttccccacc acatccgcaa cggacacctg accggcacac ccacaccctc
    20041 aatctgccac gacatcgcct cacgtcagct tgcccgcccc gacacccaag ccgtcctgac
    20101 catcctcagc ccctccggac acgaggatta caaaaaaaca gcacgcgccc ttatccgcct
    20161 gtccgaacag accgccaaac ccctgctcgt cagcagcccc ttttcagacg gcataacaca
    20221 tttcgacacc cccactcagg caatccgcac cctttcctac cgcaacaccg ccgccgccct
    20281 gaaacaggca cagctcgaca ttgcaccgcc gcagccatgc cgtctgaaaa caccgcaacc
    20341 ccaaaacatc aaaaaagccc tcgaagccgc cgaccccgcc ctaatcgccg aagccctgca
    20401 cctccccccc taccggcaca ccacccataa cgccgtccaa ttccaattcg gcagccaccc
    20461 tctctatggc gacatcctga ccgcacgctg caacggacaa accactgccg tactcccgcc
    20521 gtttaccacg ctcgacagcc gccaccttgc ccgctttgcc gaactcgacg gcacacaaac
    20581 cctcgaccag ttcctgcaca cactgaccat cattcccgaa taccgccaac acattctcgg
    20641 catcaccctc aacctcaacg gcggacaata cagcagcgat ttcatcttaa agacacccga
    20701 aacacacgac acgcccaaac gcaagaacac aggcaaagcc gcccaaaccc tcgaacatgc
    20761 cgccgcaaaa atgcagagtg ccgccgcata cctgaaacac aaaaacccga cagccgccga
    20821 atttctccgc cacacaagcg aagccgccgc agaactgctc ggcagcaaaa ccgaaaccgg
    20881 agcagccgta cccaacgtac ttgcccccta tcccgcagca cacctcaaaa cactgtccct
    20941 aaaaaacaac acgaccgtta ccattacccc ccttttgccc gaagacgcag aagccaaaca
    21001 gcagttcgtc cgcagcctca gccccgaagc acggtacaca cgcttcatga cccacaccaa
    21061 cgaactgccc gcagccacgt tggcacgcct gtgcaacccc gattaccact gtgaagccgc
    21121 atggacggca aaggatgccg acagcaacat cgtcgccgtc gtccgccaca gccgcctgaa
    21181 tcgcaacgaa tgcgaattcg gcatcacact ggcggaacat atgcgcggca gcggactggc
    21241 acagaaaatg atggaactca tcatccaaac cgccgcacag caaggctacc ggactatgag
    21301 tgccgacatt ctcaaaacca atacccccat gatcaaactt gctgaaaaat caggatttac
    21361 cctcaaggaa tcggacaccg aaaaaaacct gtaccgcgca tatctgaacc ttgcggcaga
    21421 caaaacaaca gaaaaaacaa ataaaaactt gcacaccgac cacaaaataa cctaaaatcg
    21481 gcagtttcca tatatccgat tttctcaaaa ggactcaaaa tggtagttat ccgtttggca
    21541 cgcggcggct cgaaacaccg ccccttctac aacgtcatcg ttactgactc acgcagccgc
    21601 cgcgacggcc gcttcatcga acgcgtaggc ttctacaacc ccgtagccaa cgaaaaacaa
    21661 gagcgcgtcc gcctcaacgc agaccgcctg aaccactgga ttgcacaagg cgcgcaagtc
    21721 agcgactccg ttgcaaaact gattaaagaa caaaaagccg cctaatccac atttgccgcc
    21781 atgacagaca ctcaaaaccg ggtagccatg ggctacatca aaggcgtatt cggcataaaa
    21841 ggctggttga aaattgccgc caacaccgaa tattccgaca gccttttgga ctaccccgag
    21901 tggcatttgg tcaaggacgg caaaaccatc agcattaccc ttgaagccgg aaaagtcgtc
    21961 aacggcgaac tccaagtcaa attcgaaggc ataaacgacc gcgacttggc attctcattg
    22021 cgcggttaca ccatcgaaat accccgtgaa gcattcgccc cgacagaaga agacgaatac
    22081 tactgggcag acttggtcgg catgaccgtc gtcaacaaag accataccgt tttaggcaaa
    22141 gtaagcaacc tgatggaaac cggcgcaaac gacgtattaa tgattgacgg agaacacggg
    22201 cagattctga ttccgttcgt ttcccaatat atcgaaaccg tcgataccgg cagcaagacc
    22261 attactgccg actggggttt ggactactga tgcttatcca ggcagttacc attttccccg
    22321 aaatgttcga cagcattacc cgctacggcg taacgggacg cgcgaacaga cagggaatct
    22381 ggcagtttga agcagtcaat ccccgaaagt ttgccgacaa cagattgggc tacatcgacg
    22441 accgcccgtt cggcggcggc ccgggaatga ttatgatggc tccgccgctt catgcagcaa
    22501 tagaacacgc caaagcacaa tcttcccaaa ccgcaaaagt catctacctc agcccccaag
    22561 gaaaaccgct gacacaccaa aaagcggcag aactggcaga acttccgcat ctgattctgc
    22621 tgtgcggacg ctatgaggga atagacgaaa gactgctgca aaccagcgtc gatgaagaaa
    22681 tcagcatcgg agacttcgtt gtttccggcg gagagcttcc cgccatgatg ctgatggatg
    22741 cggtattgag gctcgtaccc ggcgtattgg gcgatatgca gtctgccgaa caagattcgt
    22801 tctcaagcgg cattttggac tgcccccact acaccaaacc cttagaattt caaggtatgg
    22861 ctgttccgga agtattgcgt tccggcaatc atggcttgat agcggaatgg cggttggaac
    22921 aatcgctgcg ccgcaccttg gagcgcagac ccgacttatt ggaaaagcgc agtttaatcc
    22981 caaaggaatc ccgcctcttg gaaaccatcc ggcaagagca acgggaaatc caatcataat
    23041 ttaggaaaaa aacaatgaac ctgattcaac agctcgagca agaagaaatt gtccgcctga
    23101 acaaagaaat tcccgaattc gcaccgggcg acaccgtagt cgtatccgta cgcgtcgtgg
    23161 aaggtacccg cagccgtctg caagcctacg aaggcgttgt cattgcacgc cgcaaccgtg
    23221 gtctgaacag caacttcatc gtccgcaaaa tctccagcgg cgaaggtgtt gaacgtactt
    23281 tccaactgta ctctccgacc gtcgaaaaaa tcgaagtcaa acgccgtggt gacgtacgcc
    23341 gtgccaaact gtactacctg cgcggcctga ccggcaaagc tgcacgcatc aaagaaaaac
    23401 tgcctgcacg caaaggttga ttcaaacccg ttttccccaa tgccgtctga accttctgac
    23461 ggcatttctt attgccgcaa catccgcaac ggaacagcac caagaagcac accccttccg
    23521 gcacatcccg aatccccgtc cgaaccgaac aaaatttaca gaccaaacgt tatacagtat
    23581 catatcagcc atataataat aaccgcttta ccgctttccc tttcaaaacc gcaaatcatc
    23641 atgacacctt cccttttact atcaggattg accttccgcc tcgtccttgc cctgattgcc
    23701 gtatcccttt tatggggcgt ttacttttgg gcagtatccg catgagcatc attgtcgaaa
    23761 acctgaccgt cagctaccgc cgccgtcctg ccgtacacca cgttgacatt acttttgaag
    23821 aacatagtat gtgggcggtt ttcggtccca acggcgcagg gaaatccacc tttctcaaat
    23881 ccttgatggg attgcagcct atcgatacag gcagcatccg gctggatgga ttgagccgcc
    23941 aagacatcgc ctaccttccc cagcagtccg atatcgaccg ctcccaacct atgactgttt
    24001 tcgacttggc ggcaatgggg ctttggtatg aaatcggctt tttcaaaggg ataaataccg
    24061 ctcaaaaaca acgcgttcac gaagccttgg agcgtgtagg aatgcggcag tttgcacgcc
    24121 gccaaatcgc ccacctctca aacggacaat tccaacgcgt cctttttgcc cgaatgctgg
    24181 ttcaaaatgc aaaattcctg cttttggacg aacctttcaa cgccgttgat gcacggacga
    24241 cctacgaact tctcgacgta ttgcagaaat gccattgcgg cggacacgct atcattgccg
    24301 tactgcacga ttacgaacaa gtccgtgcct actttcccag taccctgctg ctcgcccgag
    24361 aaaaaattgc ggcaggcgca accgagacca ttctgacaga accctacctc gcccaagcca
    24421 acgccaaaat gcagcaacag gaaagccccg actggtgcgc ctcataaata ctgcccgaaa
    24481 ccacgcccca caccagacac catgaatctc tacgacctgc tccttgcccc ctttgcagaa
    24541 ttcgacttta tgcgctacgc cctcgcatcc gtcttctgcc tgtccctcag tgccgcaccc
    24601 gtcggcgtat tcctcgtcat gcgccgtatg agcctgatag gcgacgcatt gagccacgcc
    24661 gtcctgcccg gtgccgccgt cggctacatg tttgccggct tgagcctgcc cgccatgggt
    24721 ttgggcggcg tagccgcagg catgctgatg gcactgcttg ccggactcgt cagccgcttc
    24781 accaccctga aagaagatgc caactttgct gccttttatc tcagcagcct cgccatcggc
    24841 gtagtcctcg tcagcaaaaa cgggagcagc gtcgatttgc tccatctcct tttcggctct
    24901 gtacttgccg tcgatattcc tgccctgcag ctcatcgccg ccgtctccag cctcacgctc
    24961 attacccttg ccgtcatcta ccgcccgctc gtactcgaaa gcatcgaccc cctgtttctc
    25021 aaatccgtcg gcggcaaagg cgggctttgg cacgtcctct ttctcgtcct agtcgtcatg
    25081 aacctcgtat ccggctttca agccctcggc acactcatgt ccgtcggact catgatgctg
    25141 ccagccatta ccgcccgcct gtgggcgaag cacatgggcg cactcatcct cctatccgtt
    25201 ctgacagccc tgctgtgcgg cttgagcgga ctgctcattt cctaccacat cgaaattccc
    25261 tccggtcccg ccatcatcct ctgttgcagc gtcctttatc tcttttccgt catactcggc
    25321 aaagaaggcg gcattctgac caaatggctc aaaaaccacc gccaccacac cacctgaccc
    25381 cgcaagccgc ttgaacaaac aggcagcttg ttttaccata acagatgtta cgttatatca
    25441 caggagaaca gttatgaaac acctcaaact cacccttatt gccgcattgc taaccaccgc
    25501 cgcaactgcc gcccccctgc ccgtcgtaac cagcttcagc attttaggcg acgttgccaa
    25561 gcaaatcggc ggagagcgcg tatccataca aagtttggtc ggagccaacc aagatactca
    25621 tgcctatcac atgaccagta gcgacattaa aaaaatccgc agtgcaaaac tcgtcctgct
    25681 caacggctta ggacttgaag ctgccgatgt acaacgtgcc gtcaaacaga gcaaagtatc
    25741 ctatgccgaa gcgaccaaag gcatccaacc cctcaaagcc gaagaagaag gcggacacca
    25801 tcatgaccac gaccatgacc acgaaggaca ccaccatgac cacggcgaat ttgacccgca
    25861 cgtctggaac gaccccgtcc ttatgtccgc ctatgcccaa aacgtcgccg aagccctgat
    25921 aaaggccgac cccgaaggca aagtttatta tcaacaacgc ttgggcaact accaaatgca
    25981 gcttaaaaaa ctgcacagcg acgcacaagc cgcatttaac gccgtccctg ccgccaaacg
    26041 caaagtcctg accgggcacg atgccttttc ctacatgggc aaacgttacc atattgaatt
    26101 catcgccccg caaggtgtga gcagcgaagc cgagccttca gccaaacaag tcgccgccat
    26161 catccgacaa atcaaacgcg aaggcatcaa agccgtcttt accgaaaaca tcaaggacac
    26221 ccgtatggtt gaccgtatcg ccaaagaaac cggtgtcaac gtcagcggca aactgtattc
    26281 cgacgcactc ggcaacgcgc ccgcagacac ctacatcggc atgtaccgcc acaacatcaa
    26341 agccttgatc aacgcgatga agcaataaaa ccgtttgatt ggtttgaatt tgaaaaggca
    26401 aaaggccgtc tgaaaattat agtggattaa caaaaatcag gacaaggcga cgaagccgca
    26461 gacagtacag atagtacggt aaggcgaggc aacgctgtac tggtttttgt
//
LOCUS       DAWFHY010000020        25524 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.020, whole genome shotgun sequence.
ACCESSION   DAWFHY010000020 DAWFHY010000000
VERSION     DAWFHY010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25524
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.020"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(508..702)
                     /locus_tag="WGQ68_000757"
     CDS             complement(508..702)
                     /locus_tag="WGQ68_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEZ0714600.1"
                     /translation="MKHKLASWWKTIKLTANLMAGVPDYENYVAQQRKHNPNAPVMTK
                     LQFQDYCRKRRCGANGGRCC"
     gene            complement(692..2773)
                     /locus_tag="WGQ68_000758"
     CDS             complement(692..2773)
                     /locus_tag="WGQ68_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein A"
                     /protein_id="HEZ0714601.1"
                     /translation="MKSLKTFLIWGIVVLVGLASFTTLALSRGEQVSAVWMVTAAISV
                     YCIAYRFYSLYIANHVMRLDPNRLTPAERHNDGLDYVPTHKGVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGTLWIIFGVVFAGAVQDMMVLFVSMRRDGKSLGDIVKQELGTVPG
                     VIASIGILMIMVIIMAVLALIVVKALVHSPWGTFTIAATMPIALFMGIYTRYIRPGKI
                     GEISIVGFILLMLAVIYGEDVAKSSIGHWFDLDGIQLTWAIMIYGFVASVLPVWLLLT
                     PRDYLSTFLKIGTIAALALGIVIVNPALQMPAVTHFIDGSGPVFSGALFPFLFITIAC
                     GAVSGFHALISSGTTPKMLENETHVRMIGYGGMLMESFVAIMALAAAASLDPGVYFAM
                     NSPAALIGTDANTAAEVITTKLQFPVDAATLLHTAKEVGENTILSRAGGAPTLAVGMA
                     HIMSRLIPGEAMMAFWYHFALLFEALFILTAVDAGTRVARFMIQDLGSIFYKPFGNTD
                     SIPANLIATFFAVALWGYFLYTGVTDPLGGINSLWPLFGIANQMLAGVALIMCAVVLI
                     KMKRDRYVWVVLVPAVGVLFVTCYAGLQKLFHSDPRISFLAHAGKYSDALAKNEVLAP
                     AKDIGEMAQIIFNDKINAGLTILFLSVVVIVAAYGLRTALKARKVGWPTAKEIPAVYR
                     DGKQPEAQSEA"
     gene            3249..3629
                     /locus_tag="WGQ68_000759"
     CDS             3249..3629
                     /locus_tag="WGQ68_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HEZ0714602.1"
                     /translation="MNIRFFALTVPVLSLAACAVPEAYDDGGRGHMPPVQNQVGTDDF
                     RAFSCENGLSVRVRHLDSGKVALRLDGRRAVLSSDVAASGERYTAEHGLFGNATEWHQ
                     KGGEAFFGFTDAYGNSVETSCRAR"
     gene            complement(3711..3898)
                     /locus_tag="WGQ68_000760"
                     /pseudo
     CDS             complement(3711..3898)
                     /locus_tag="WGQ68_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4440..5873
                     /locus_tag="WGQ68_000761"
     CDS             4440..5873
                     /locus_tag="WGQ68_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEZ0714603.1"
                     /translation="MNEYSQLIKHPDISLPPVSDGIKVDNPATGETLAFVRKTDSDKL
                     KNLIQKAAAAQKLWAAKTALERADVLWRWYFQIKENKEALARLMTMEQGKSLTEARGE
                     IDYAASFVRWFAEEARRIDGDVLTSVKASQKLVVLKQPVGVTAAITPWNFPSAMIARK
                     AAPALAVGCAMIVKPASLTPLSAYALASLAYEAGIPQDLLPVVSGSASEIGHEFATNP
                     IIRKISFTGSTEVGAKIFADSAADIKKLSLELGGNAPFIVFDDADLDKAVEGALASKF
                     RNSGQTCVCTNRVYAQSAIYDEFCRKLSEKAAALKLGNGLEDGVNQGPLIEEKAVEKV
                     EQHIADALAKGASCLTGGKRSALGGTFFEPTVLSGVTAQMAVAREETFGPLCPVFRFE
                     TEAEVIEAANNTEYGLAAYLFTADTARQWRVGEALEYGMVGINTGLISNEAAPFGGVK
                     RSGLGREGSKYGADEYLELKYLCMDVG"
     gene            5982..6770
                     /locus_tag="WGQ68_000762"
     CDS             5982..6770
                     /locus_tag="WGQ68_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimb protein"
                     /protein_id="HEZ0714604.1"
                     /translation="MMENGKTVPRWRFALKSAGWHLLISLSVAGLAALLVFKVWYPYP
                     YAELTGGLSLYQLVVAVDIVCGPLLTLILASPKKKTKARMVDFSMVGIIQLAALVYGL
                     HSVSLARPVVEAFEQDRMTIVTAAEVVVEDLHKAPEGLQSLSWFGIRRIALKEPEDAD
                     EKNKTLDLSLKGIEPSMRPDQWLPYSDKEAEEIRKHLKPLKVLADARKTTVADILKQA
                     GLAEGEELYYLPFTSSRQKEWIVITDKEGNTKGYAPIDGFIITP"
     gene            7138..8694
                     /locus_tag="WGQ68_000763"
     CDS             7138..8694
                     /locus_tag="WGQ68_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0714605.1"
                     /translation="MDFSWLAEPHTWIGFATLLVLEVVLGIDNLVFVAILANKVQPAR
                     RDRARIIGLGLAVVIRIIMLAFMAHIITLTEPLFQIGGLAVSGKDMIMLAGGIFLLYK
                     ATTELHERLEGHNRFTVADSQKKHAPFWGVVAQILILDAVFSIDSVITAVAMVDHIVV
                     AMGAVVVAMAVMISASKLLTEFVDRHPTVVMLCLGFLLMIGFSLIAEAFHFHIPKGYL
                     YAAIGFSILIELFNQISQRNSRKNDYIGSSWRKRTAENVLGMMGIRESVLADAGGESG
                     DDAHFEENEKSMIRSVLTLAERPIMGVMIPRRDIERLDISQSREEQYAQLQNTPYSRL
                     LVVGKAGVDEPLGYINKKDLLSQLLETGGLDIQTALRQPLVLPDGTTALGALELFRQS
                     SADYALVVDEFGAVLGMVTMKDLLETIAGEFPEEFEREEEPAVQGNPDESLTVEGALE
                     YVELASQLNLPQQEEDADFHTVAGLIMEELQTIPDVGDFADFHGWRFEVVEKEGQRIE
                     RVKITKLPEE"
     gene            8711..9457
                     /gene="surE"
                     /locus_tag="WGQ68_000764"
     CDS             8711..9457
                     /gene="surE"
                     /locus_tag="WGQ68_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEZ0714606.1"
                     /translation="MNVLISNDDGYLSEGIAVLARVTAEFANVRVVAPERDRSGVSNS
                     LTLERPLQLKQAQNGFYYVNGTPTDCIHIGQSVFSDFQADFVFSGINRGANMGDDTLY
                     SGTVAAATEAYLMGIPAVAFSLNDASGRYWATAEKALWTLLAHFFKTPPQFPILWNIN
                     IPAVAPEDVRGIKIARLGRRHHGQNVIPARNPRGEQIYWIGPVGRVSDKEEGTDFGEC
                     GAGFITVTPLQIDLTAYPDMAETAAFWHAD"
     gene            9713..10960
                     /locus_tag="WGQ68_000765"
     CDS             9713..10960
                     /locus_tag="WGQ68_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0714607.1"
                     /translation="MLKQTTLLAACASFAALLGGCATQQPAPVIAGNSGMQDAPSSAV
                     YNNPYGATPYSPAPAGDAPYVPPVQSAPVYTPPAYVPPSAPAVSGTYVPSYAPVDINA
                     ATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNTLSIGQIVKVKPAGYAAPKAAAVK
                     SRPAVPAAAQPPVQSAPVDINAATHTIVRGDTVYNISKRYHISQDDFRAWNGMTDNML
                     SIGQIVKVKPAGYAAPKTAAVESRPAVPAAVQTPVKPAAQPPVQSAPQPAAPAAENKA
                     VPAPAPQSPAASPSGTRSVGGIVWQRPTQGKVVADFGGNNKGVDIAGNAGQPVLAAAD
                     GKVVYAGSGLRGYGNLVIIQHNSSFLTAYGHNQKLLVGEGQQVKRGQQVALMGNTDAS
                     RTQLHFEVRQNGKPVNPNSYIAF"
     gene            complement(11167..11649)
                     /locus_tag="WGQ68_000766"
     CDS             complement(11167..11649)
                     /locus_tag="WGQ68_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0714608.1"
                     /translation="MTQQRQLPSHELIMSELMMPDTANFSGNVHGGELLLLLDQVAYS
                     CASRYSGNYCVTLSVDKVLFKEPIHIGDLVTFYAAVNYTGRTSMEIGIRVEAQNIRTG
                     EVRHTNSCYFTMVAVKDGKPVPVPPLEILTDRQRCRYEKAKKRRDISLQASEDMSCGC
                     "
     gene            complement(11802..11963)
                     /locus_tag="WGQ68_000767"
     CDS             complement(11802..11963)
                     /locus_tag="WGQ68_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714609.1"
                     /translation="MSGQPEKHHTSPIEDERKNPVYRMGRAVAGFMLVVWVAVLALVF
                     FLVFRFWLS"
     gene            complement(12037..12498)
                     /gene="recX"
                     /locus_tag="WGQ68_000768"
     CDS             complement(12037..12498)
                     /gene="recX"
                     /locus_tag="WGQ68_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEZ0714610.1"
                     /translation="MKPQKSLRARAMDILSRQELSRIGLKRKLAPHAESEEELENVLN
                     EFAERNWQSDLRYAEAYIRSKSRKHGSLRLKQALAQQGIDEETSRNLLPDRSSEKLAA
                     IAVLRKKFKHPAADLKEKQKQARFLAYRGFDADTVQTALKHAWDENWEDGC"
     gene            complement(12566..13273)
                     /locus_tag="WGQ68_000769"
     CDS             complement(12566..13273)
                     /locus_tag="WGQ68_000769"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HEZ0714611.1"
                     /translation="MNAAIEHVQAVAFDLDGTLCDSVPDLAAAAEAMLEQLGMKPLPA
                     KVVESYVGDGIGKLVHRVLTNDRDREADSELWEKGFVSYMKYYRDHLSVFTRPYPETE
                     AGLALLKSLGIPLAVITNKNEILAVELLKQLGLADYFSLILGGDSLPEKKPSPLPLRH
                     AAEVLGIDVANMVMVGDSRNDIIAAKAAGCLSVGVTFGYGDMTLLSQDDATRPDWIIG
                     SLPEIYENLQPQKNKEE"
     gene            13680..14945
                     /locus_tag="WGQ68_000770"
     CDS             13680..14945
                     /locus_tag="WGQ68_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003420866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEZ0714612.1"
                     /translation="MSEALAKETLNPFEIARKQVKTACDRLKTDPAVYEILKSPTRVL
                     EVNFPVKLDDDTVKTFTGYRSQHNNAVGPYKGGVRFHPGVNLDEVKALSIWMTIKCCV
                     AGIPYGGGKGGVTLDPRDYSEAELERIARAYSEAISPLIGEKIDIPAPDVNTNGKIMS
                     WMVDAYENVVKHSAPGVFTGKPVEFGGSLARTEATGYGVNLAAVQALEKLGKDVKGAT
                     YAIQGFGNVGYHTGYYAHQSGAKVVAVSTVDVAIYNENGLDMEAIIKEFQTKGFITNE
                     AGYGKEISNAELLALEVDVLAPCALENQLTSENAGKVRATIVVEGANGPTTPEADVIL
                     RQNGVLVVPDILANCGGVVVSYFEWVQNLQGYYWEFDEVQEKETVVLRRAFRDIWNLA
                     QEYDVDLRTASYMMSIRRVEKAMKLRGWY"
     gene            complement(15003..15809)
                     /locus_tag="WGQ68_000771"
     CDS             complement(15003..15809)
                     /locus_tag="WGQ68_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HEZ0714613.1"
                     /translation="MKKTLTLLAVSALFATSAHAHRVWVETAHTHGGEYLKADLGYGE
                     FPELEPIAKDRLHIFSKPMQLVTEKGKENMIQRGTYNYQYRSNRPVKDGSYLVIAEYQ
                     PTFWSKNKAGWKQAGIKEMPDASYCEQTRMFGKNIVNVGHESADTAIITKPVGQNLEI
                     VPLDNPANIHVGERFKVRVLFRGEPLPNATVTATFDGFDTSDRSKTHKTEAQAFSDST
                     DDKGEVDIIPLRQGFWKANVEHKADFPDQSVCQKQANYSTLTFQIGHSHH"
     gene            16154..16363
                     /locus_tag="WGQ68_000772"
     CDS             16154..16363
                     /locus_tag="WGQ68_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HEZ0714614.1"
                     /translation="MPYVNIKVTGGKEAPTAAQKAELIGGVTELLARVLGKNPETTVV
                     VIDEVDTDNWGIGGKSVSERRKEGR"
     gene            16452..17666
                     /locus_tag="WGQ68_000773"
     CDS             16452..17666
                     /locus_tag="WGQ68_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEZ0714615.1"
                     /translation="MDKFPKSAKLDHVCYDIRGPVHKKALQLEEEGNKILKLNIGNPA
                     PFGFEAPDEILVDVIRNLPTSQGYCDSKGLYSARKAIVHYYQTKGLRDITVDDVYIGN
                     GVSELITMSMQALLNDGDEILIPAPDYPLWTAAATLAGGTVRHYLCDEENGWFPNLAD
                     MEAKITPKTKAIVVINPNNPTGAVYSREILLEIAELARKHGLIIFADEIYDKILYDGA
                     VHHHIAALAPDLLTVTFNGLSKSYRVAGFRQGWMVLNGPKHHAKGYIEGLDMLSSMRL
                     CANTPMQHAIQTALGGYQSINEFILPGGRLLEQRNRAWELVSQIPGVSCVKPMGAMYM
                     FPKIDTEMYRIHDDMKFVYDLLVREKVLLVQGTGFNWIKPDHFRIVTLPYVHQIEEAM
                     GRLARFLQTYRQ"
     gene            complement(17753..20332)
                     /gene="clpB"
                     /locus_tag="WGQ68_000774"
     CDS             complement(17753..20332)
                     /gene="clpB"
                     /locus_tag="WGQ68_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEZ0714616.1"
                     /translation="MRYDKLTAKFQQALAEAQSLALAADGSYLEAGFVLKALLDDQNS
                     GAAALLAHSGVNVPQVKQRLQQHLSSLPKVSGQGGDIMPSRELQAVLNLMDKAATKRG
                     DVYIASELFLLALVQQNDATGKILKEAGATEQNINAAIDAVRGGQNVNDANAEDQRDA
                     LKKYTLDLTQRARDGKLDPVIGRDDEIRRAIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPESLRNKRLLVLDLAALIAGAKYRGEFEERLKGVLNDLAKDDGNTLIF
                     IDEIHTLVGAGKTDGAMDAGNMLKPALARGELHCIGATTLDEYRQYIEKDAALERRFQ
                     KVLVGEPSVEDTIAILRGLQERYEIHHGIDITDPAIVAAAELSDRYITDRFLPDKAID
                     LIDEAASRVKMEKETKPEAMDKIDRRLIQLRMEKAHVEKEKDDASKKRLELIDEEING
                     LQKEYADLDEIWKAEKAISDGAANIKKQIDEVKIKIEQAKRQGDLALASKLMYEDLEH
                     LEKQRAAAERADTDSTKPANKLLRNNVGAEEIAEVVSRMTGIPVSKMMEGERDKLLKM
                     EEVLHRRVVGQDEAVRAVSDAIRRSRSGLADPNKPYGSFLFLGPTGVGKTELCKALAG
                     FLFDSEDHLIRIDMSEYMEKHAVARLIGAPPGYVGYEEGGYLTEQVRRKPYSVILLDE
                     VEKAHPDVFNILLQVLDDGRLTDGQGRTVDFKNTVIVMTSNIGSQHIQQMGIQDYEAV
                     KEVVMEDVKEHFRPEMINRIDEVVVFHGLDQANIRSIAKIQLKGLEKRLEKQNLRLTV
                     SDAALDIIAKAGFDPIYGARPLKRAIQSEIENPLAKALLAGNYAPESEIRVEADGDRL
                     KFA"
     gene            20592..21602
                     /gene="trpS"
                     /locus_tag="WGQ68_000775"
     CDS             20592..21602
                     /gene="trpS"
                     /locus_tag="WGQ68_000775"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEZ0714617.1"
                     /translation="MSKKRVLTGVTTTGIPHLGNYVGAIRPAVRAAQNPDTESFLFLA
                     DYHGIIKCHEPEMIHQSTQAVAATWLACGLDPERTTFYRQSDIPEVMELNWILTCITA
                     KGLMNRAHAYKAAVQANSENNQEDPDFGVEMGLFSYPILMTADILMFNANEVPVGRDQ
                     IQHVEMARDIAGRFNHRFKEIFTLPEVKIDENVELLVGLDGRKMSKSYGNTIPLWEND
                     KKTQKSVNKIITNMKEPGEPKQPDESPLFEIYKAFSTPSETAAFTQMLAEGLAWGEAK
                     KLLAAKINAELAEPRERYNELTADPSQIEEILQAGAAKARKEARELLDKVRDAVGIRP
                     LK"
     gene            21670..22215
                     /locus_tag="WGQ68_000776"
     CDS             21670..22215
                     /locus_tag="WGQ68_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714618.1"
                     /translation="MLKTSFAVLGGCLLLAACGKSENTAEQPQNAVQSAPKPVFKVKY
                     IDNTAIAGLDLGQSSEGKTNDGKKQISYPIKGLPEQNVVRLTGKHTNDLEAVVGKCME
                     TDGKGAPSGWAANGVCHTLFAKLVGNIAEDGGKLTDYLISHSALQPYQAGKSGYAAVQ
                     NGRYVLEIDSEGAFYFRRRHY"
     gene            22534..22857
                     /locus_tag="WGQ68_000777"
     CDS             22534..22857
                     /locus_tag="WGQ68_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714619.1"
                     /translation="MKKLLIAAMMAAALAACSQEAKQEVKEAVQAVESDVKDTAASAA
                     ESAASAVEEAKDQVKDAAADAKASAEEAVTEAKEAVTEAAKDTLNKAADATQEAADKM
                     KDAAK"
     gene            complement(23066..24574)
                     /gene="ppx"
                     /locus_tag="WGQ68_000778"
     CDS             complement(23066..24574)
                     /gene="ppx"
                     /locus_tag="WGQ68_000778"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HEZ0714620.1"
                     /translation="MTTTPANILASVDLGSNSFRLQICENNNGQLKVIDSFKQMVRFA
                     AGLDEQKNLSASSQEQALDCLAKFGERLRGFRPEQVRAVATNTFRVAKNIADFLPKAE
                     AALGFPIEIIAGREEARLIYTGVIHTLPPGGGKMLVIDIGGGSTEFVIGSTLNPDITE
                     SLPLGCVTYSLRFFQNKITAKDFQSAISAARNEIQRISKNMRREGWDFAVGTSGSAKS
                     IRDVLAAEMPQEADITYKGMRALAERIIEAGSVKKAKFENLKPERIEVFAGGLAVMMA
                     AFEEMKLDSMTVTEAALRDGVFYDLIGRGLNEDMRGQTVAEFQHRYHVSLNQAKRTAE
                     TAQTFMDSLCHAKNVTVQELALWQQYLGRAAALHEIGLDIAHTGYHKHSAYILENADM
                     PGFSRKEQTILAQLVIGHRGDMKKMSGIIGTNEMLWYAVLSLRLAALFCRSRQDLSFP
                     KNMQLRTDTESCGFILRIDREWLERHPLIADALEYESVQWQKINMPFKVEVV"
     gene            complement(24690..25422)
                     /locus_tag="WGQ68_000779"
                     /pseudo
     CDS             complement(24690..25422)
                     /locus_tag="WGQ68_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629272.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
BASE COUNT         5870 a         6423 c         7068 g         6163 t
ORIGIN      
        1 cggaaactta tcggataaaa cggtttcttc agattttacg ttctggattc ccactttcgt
       61 gggaatgacg ggatgtaggt tcgtaggaat gacgtggtgc aggtttccgt gcggatggat
      121 tcgtcattcc cgcgcacgcg ggaatctaga ccttagaaca acagcaatat tcaaagatta
      181 tctgaaagtc cgagattcag attcccgcgt tcgcgggaat gacgaaaagt ggcgggaatg
      241 acggttcggg cattccttaa attacccgtg tatcgctgta aatcttagag atggcggaat
      301 atagcggatt aacaaaaacc agtacggcgt tgtctcgcct tggctcaaag agaacgattc
      361 tctaaggtgc tgaagcacca agtgaatcgg tttcgtacta tttgtactgt ctgcggcttc
      421 gtcgccttgt cctgattttt gttaatccac tatattataa aaattccgtc tgaagcggcg
      481 ttcagacgga atttgtttca agcaggctta acagcagcgt ccgccgtttg cgccgcagcg
      541 gcgtttgcgg caatagtctt gaaactgcag cttggtcatc acaggggcgt tgggattatg
      601 tttgcgttgt tgcgcaacgt agttttcata atcgggcaca cctgccatca agtttgccgt
      661 cagcttgatg gttttccacc aagacgcgag cttatgcttc actttgtgcc tccggctgtt
      721 tgccgtcgcg gtacaccgcc gggatttctt tggcggtcgg ccagccgact ttgcgtgctt
      781 tgagggcggt acgcaaaccg tacgcggcga caatcacgac aaccgacaag aagaggatgg
      841 tcagaccggc attaatcttg tcgttgaaga tgatttgcgc catttcgccg atgtctttag
      901 cgggcgcgag gacttcgttt ttagccaatg cgtcgctgta tttgccggcg tgggcaagga
      961 agctgatgcg cgggtcgctg tggaacagtt tttgcaggcc ggcgtagcag gttacaaaca
     1021 gtacgccgac ggcgggaacg agtaccaccc agacataacg gtcgcgtttc atcttaatca
     1081 gcaccacggc gcacataatc aaggctacgc ctgccagcat ttggttggcg atgccgaaca
     1141 aaggccagag cgagttgatg ccgcccagcg ggtcggttac gccggtgtag aggaagtagc
     1201 cccacaatgc cacggcgaag aaggtcgcaa tcaggttggc agggatggag tcggtgttgc
     1261 cgaaaggttt gtagaagatg ctgcctaagt cttgaatcat aaaacgtgcg acgcgcgtac
     1321 cggcatcgac ggcggtcagg atgaacaagg cttcaaacaa cagggcgaag tgataccaga
     1381 acgccatcat cgcctcgccc ggaatcaggc ggctcataat gtgcgccata ccgactgcga
     1441 gggtgggcgc accgccggca cgggaaagga tggtgttttc gcccacttcc ttagcggtgt
     1501 gcaacagggt tgccgcatcg acagggaatt gcagcttggt ggtaatcact tcggcggcgg
     1561 tattggcatc cgtaccgatc agggcggcag ggctgttcat ggcgaagtac acgccgggat
     1621 caagcgatgc ggcggcggca agcgccataa tggctacgaa actttccatc aacataccgc
     1681 cgtaaccaat catacggacg tgggtttcgt tttccagcat tttcggcgtg gtgccggagg
     1741 aaatcagcgc gtggaagccc gaaaccgcac cgcaggcgat ggtgatgaag aggaacggga
     1801 acaatgcgcc tgagaatacc ggacccgaac cgtcgataaa gtgggttacg gcaggcattt
     1861 gcaaagcggg attgacgatg acgatgccca aagccaaggc cgcaatcgta ccgattttca
     1921 ggaaggtgga gagatagtcg cgcggagtga gcagcaacca tacgggcaat acggaggcga
     1981 caaagccgta aatcataatc gcccaagtga gctggatgcc gtcaaggtcg aaccaatgtc
     2041 cgatggaact tttcgccaca tcttcgccgt aaattaccgc cagcatcagc aaaataaagc
     2101 cgacgatgga aatctcgccg attttgcccg gacggatata gcgcgtgtaa atacccataa
     2161 acagggcaat cggcatagtg gcggcgatgg taaacgtacc ccaagggctg tgaaccagtg
     2221 cttttacgac aatcaacgcc aacaccgcca taatgatgac cataatcatc aaaataccga
     2281 tggaggcaat cacgccgggg acagtgccga gttcctgttt cacaatatcg cccaaagact
     2341 taccgtcgcg gcgcatagag acgaacaaga ccatcatatc ctgtaccgcg ccggcaaaca
     2401 ccacgccgaa gataatccac aaagtaccgg gcaggtaacc catttgcgcc gccaaaaccg
     2461 ggccgaccaa agggcccgcg cccgcaatcg cggcaaagtg gtgtccgaac aatacgccct
     2521 tgtgcgtcgg aacgtagtcc aagccgtcgt tgtggcgttc tgccggagtc aggcggttag
     2581 gatcgagccg cattacgtgg ttggcgatgt agaggctgta aaaacggtag gcgatgcagt
     2641 aaacggatat ggcggcggta accatccata ccgcgctgac ctgttcgcct cggctgagag
     2701 ccagagtggt aaaggatgct aagccgacca gtaccactat gccccaaatg aggaaggttt
     2761 tgagtgattt catcgaataa atccttatct cacactgtcg gaatatgcct gaacggcggg
     2821 aaacggatga ccgcctgccg gcacgggcac aaaccggagt ctgaatgatt gacgaagtat
     2881 gaaacagtgc gcttgtcggg atttgtgcct tatgcccgca tcaaacagtg cagggatgcg
     2941 ggcgcacaaa acgttaaacg ccgaatagga ttttaacgca aattagcaca ctgatagcgg
     3001 tttttacttg gaatttggaa aaaatttaca ttccttcggg cggacaggcg ggtttggacg
     3061 gcatcgtcag gcaaaaggcg gcgccggaag acgggtaaag aaggggcgcg caatccggat
     3121 tattgattca tcgcgggtaa attccggtta tcgggcttgt gtgtttgcgc gtccgtttat
     3181 agtatggcgt tgccgcagct tggaatcagg gcggttgttt catatcttat ttttattggg
     3241 gagcttttat gaatatcagg tttttcgcgc tgaccgtacc ggttttgtct ttggcggcct
     3301 gtgccgtgcc ggaggcgtat gatgacggcg gacgagggca tatgccgccc gttcaaaacc
     3361 aagtcggcac ggacgatttt cgggcgtttt cctgcgagaa cggtttgtct gtgcgcgtcc
     3421 gccatttgga cagcggcaaa gtcgcgttgc ggctagacgg caggcgtgcc gtcctctctt
     3481 ccgacgttgc cgcatccgga gaacgctata ccgccgaaca cggtttgttc ggaaacgcaa
     3541 ccgagtggca ccagaaaggc ggcgaagcct ttttcggctt taccgatgcc tacggcaatt
     3601 cggtcgaaac ttcctgccgc gcccgttaaa cggggcagcc tgtttgaacc gccgtccgga
     3661 tttttcgggc ggttttttgt gtcggacgga cggttttttg tgtcggattt gttttgcagt
     3721 tcggcctccg gcagggttcg ggctgcctga taccgtccgt aacttcgggt attgccgagt
     3781 tcgtcggcaa cggccgctgc ggcttgggcg gcgttttgcc gaacgcctgc gttacttccc
     3841 tttgtaggtc gatctctttg gcaaccgcgt ctttgtcaaa gctgtttttc agacgacctt
     3901 ttatcaaata ttatcggcag cggctcaatg ccaactttaa acctgctccg atttcttcag
     3961 ggctgttatc caatgataaa atatagtgga ttaacaaaaa tcaggacaag gcgacgaagc
     4021 cgcagacagt acagatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc
     4081 gttctctttg agctaaggcg aggcaacgct gtactggttt ttgttaatcc actataatgt
     4141 ttggattttt gccatactct accacacgtt gcaactgcaa tctttgctct ttatatattg
     4201 caccccatca aagggctgca ttacttttct ttctaaagat tactatcata taaaaaatat
     4261 tttttaaacg acagggattg cgcccgcgcc atattcaaac actgcccgga tgttgcgctg
     4321 cgcgatcgga tgcttcagat ggcatcggaa gggtttgcag ttttggaata tgaagatgat
     4381 aatgtccgcg agattgacgg catttgaaag cggttattcc gcataccgga ggatacgaaa
     4441 tgaacgaata ttcccaatta atcaagcatc ccgatatttc ccttcccccg gtttcagacg
     4501 gcatcaaagt cgataatccg gcaacgggcg agactttggc gtttgtccgc aagacggatt
     4561 cggacaagct gaaaaacctg attcaaaaag cagctgcagc acaaaaatta tgggcggcaa
     4621 aaactgcgtt ggaacgcgcc gatgtgttgt ggcgttggta ttttcagatt aaagaaaaca
     4681 aagaagcatt agcgcgcctg atgacgatgg agcagggcaa aagcctgacc gaggcgcgtg
     4741 gcgaaatcga ttatgcggct tcgtttgtgc gctggtttgc cgaagaggcg cggcggattg
     4801 acggcgatgt gctgacgagt gtgaaagcgt cgcaaaaact ggtcgtgttg aaacagcccg
     4861 tcggcgttac cgctgcgatt acgccgtgga actttccgtc cgcgatgatt gcgcgcaagg
     4921 ccgcgcctgc tttggcggtg ggttgcgcga tgatcgtcaa acccgcatcg ctcacgcctt
     4981 tgagtgcgta tgccttggct tcgttggctt acgaagcggg cataccgcag gatttgttgc
     5041 ctgttgtcag cggcagtgct tcggaaatcg gccatgaatt tgctacgaac ccgattatcc
     5101 gcaaaatcag cttcaccggc tcgaccgaag tcggcgcaaa aatttttgcc gacagcgcgg
     5161 cggacattaa aaaactcagt ttggagctgg gcggcaacgc gccgtttatc gtgtttgacg
     5221 atgccgattt ggacaaagcc gtcgaaggcg cgctcgccag caagttccgt aacagcggtc
     5281 agacctgcgt ctgcaccaac cgcgtttacg ctcaatccgc catttacgac gaattttgcc
     5341 gcaaattgag tgaaaaagca gccgcgctca aattgggcaa cggcttggag gatggtgtga
     5401 accaagggcc gctgattgag gaaaaagcgg tggagaaagt cgagcagcac atcgccgacg
     5461 cgcttgctaa aggtgcaagc tgcctgaccg gcggcaaacg cagcgcgttg ggcggaacgt
     5521 ttttcgaacc gactgtttta agcggcgtaa cggcgcaaat ggcggtggca cgcgaagaaa
     5581 ccttcgggcc gttgtgtccg gtattccgtt ttgaaaccga agccgaggtc atcgaggctg
     5641 cgaacaatac ggaatacggt ttggcagctt accttttcac cgccgacacc gcccgccaat
     5701 ggcgcgtcgg cgaagccttg gaatacggta tggtcggcat caatacgggc ttaatcagca
     5761 atgaagcggc accgttcggc ggcgtgaaac gtagcggttt gggacgtgaa ggcagcaaat
     5821 acggtgcgga cgaatatcta gaattgaaat atctgtgtat ggatgtcggg tgacggatgc
     5881 cgtctgaacg gcaggatttc agacggcgcc gcattttaag cagtctctat ctgttgtaca
     5941 atgcgccctg tttttacggt tatttttgat ttgaacaaga tatgatggaa aacggaaaaa
     6001 cagtcccgag atggcgtttt gccttgaaaa gtgcgggctg gcacctctta atcagcctgt
     6061 cggttgcagg gctggcggca ttgctggttt ttaaggtttg gtatccttat ccttatgccg
     6121 agctgacggg agggctgtcg ctttatcagc tggtggtggc tgtcgatatt gtatgtggtc
     6181 cgctgctgac tttaattttg gcaagcccga agaaaaagac aaaggcacgc atggtcgatt
     6241 tttccatggt cggcatcatc cagctggcgg ctttggtgta cggtctacac agcgtttcgc
     6301 tggcgcgtcc tgtggtggaa gcgtttgaac aggatcgtat gaccattgtt acggcggcgg
     6361 aagtcgtggt cgaagatttg cacaaagccc ccgaagggct gcaaagcctg tcgtggttcg
     6421 gcatccgccg cattgcattg aaagaacctg aggatgcgga tgagaagaac aagacgctgg
     6481 atttgtccct gaaaggtatc gagccgagta tgcgtcccga ccagtggctg ccgtattccg
     6541 acaaggaagc agaagaaatc cgcaaacatc tgaaaccgct gaaagtcttg gcggatgcga
     6601 gaaaaacgac ggttgcggac attctgaaac aggcaggtct cgccgaaggg gaggagctgt
     6661 attacctgcc gtttaccagc agcaggcaga aagagtggat agtcattacc gataaagagg
     6721 gcaacaccaa aggctacgcg ccgatagacg gcttcatcat caccccttaa gcgttgggac
     6781 tccgtccgca ctcgaacatc cgttcttcgc ggcggtagaa tcagactgta tttgagaggg
     6841 gcgcaacccc tcaatcaggg caacacatag gggaaggctt tatgtgccgt ccgtgtgttg
     6901 aaacatttca aaataagctc atgccgtccg aacatccttt cagacggtat ggcgttttgc
     6961 cattgccccg ataagctgtt aaactattta aattatttcg accgaaggta cacgcacatg
     7021 caagaccccg agcagagcag caagcccgcc cgccgttttt tgtgcgtacc gtctgttcag
     7081 gcagcttgag cgcttcaggc tgccttttcg cgtttgaact tgaggaatac gaaaatcatg
     7141 gatttcagtt ggttggcaga accgcatacc tggataggtt ttgccacgct tttggtgttg
     7201 gaagtcgtat tggggataga caatcttgtc tttgtggcga ttttggcaaa caaggtccag
     7261 cccgcacggc gcgaccgcgc acggattatc gggctggggc tggcagtcgt catccgcatc
     7321 attatgcttg cttttatggc gcacatcatc acgctgaccg agccgctgtt ccaaatcggc
     7381 ggcctcgccg tttccggcaa ggacatgatt atgctcgcgg gcggtatttt cctgctttac
     7441 aaagccacca ccgaactgca tgaacgcctc gaagggcaca accgttttac cgttgccgac
     7501 agccaaaaaa aacacgcgcc gttttggggc gtggtcgcgc aaatcctgat actggatgcc
     7561 gtgttttcca tcgattcggt cattactgcg gtggcgatgg tcgatcatat cgtcgtggcg
     7621 atgggtgcgg tcgtcgtcgc gatggctgta atgatttctg ccagcaaact cttgaccgaa
     7681 tttgtcgaca gacaccctac cgtcgtgatg ctctgccttg gttttttgtt gatgatcggt
     7741 ttcagcctga ttgccgaagc cttccatttc cacattccca aaggctacct ctacgccgcc
     7801 atcggcttct cgattttaat cgaactgttt aaccagattt cccagcgcaa cagccgcaaa
     7861 aacgactaca tcggcagctc gtggcgcaaa cggactgctg aaaatgtctt gggtatgatg
     7921 ggcatacgcg aaagcgtgct tgccgacgcg ggcggcgaat ccggggacga cgcgcatttt
     7981 gaagaaaacg aaaaatcgat gatacgcagc gtgctgacgc ttgccgaacg cccgattatg
     8041 ggggtgatga tcccacgccg cgacatcgaa cggctggaca tttcccaaag ccgcgaagaa
     8101 cagtatgccc aactgcaaaa cacgccttac agccgcctgc tcgttgtcgg aaaggcgggc
     8161 gtggacgaac ctttgggcta catcaacaaa aaagacctgc tgtcccaact gctggaaaca
     8221 ggcggtctcg acattcagac ggcattgcgc cagccgctcg tcctacccga cggcacgacc
     8281 gccttgggcg cgctcgaact cttccgccaa agcagcgcgg attatgcttt ggtggtggac
     8341 gagttcggcg cggtattggg catggtaacc atgaaagacc tgctcgaaac catcgcaggc
     8401 gagttccccg aagaatttga gcgcgaagaa gaaccagccg ttcaggggaa tcccgatgaa
     8461 agcctgacgg tggaaggcgc gttggaatat gtggagctgg catcccaact caatctgccg
     8521 cagcaggagg aagatgccga tttccatacg gttgccgggc tgattatgga agaattgcaa
     8581 accatccccg atgtcggcga ttttgccgat ttccacggct ggcggtttga agtggtcgaa
     8641 aaagaagggc agcgcatcga gcgggtcaaa atcaccaaat tgcccgaaga ataagcattc
     8701 aggatagaaa atgaacgttt tgatttccaa cgacgacggc tacctctccg aaggtattgc
     8761 cgttttggcg cgcgttacgg cggaatttgc caacgtcagg gtggttgcgc ccgaacgcga
     8821 caggagcggg gtcagcaatt cgctgacgct ggaacgccct ttgcagttga aacaggcgca
     8881 aaacgggttc tactatgtca acggcacgcc gaccgactgc atccacatcg ggcagtctgt
     8941 attttcggat tttcaggccg attttgtctt ttcgggcatc aaccggggcg cgaatatggg
     9001 ggacgacacg ctttattcgg ggacggttgc ggcggcaacc gaagcctacc ttatgggcat
     9061 acccgctgtg gcgttttcct taaacgacgc ttccggacgc tattgggcaa ccgcagaaaa
     9121 ggcactgtgg acattgttgg cgcatttttt caaaaccccc ccgcagttcc ctattttgtg
     9181 gaacatcaat atccccgccg tcgcgccgga agatgtgcgg ggcattaaaa tcgcccgttt
     9241 gggcaggagg catcacggtc agaacgtcat ccccgcgcgc aatccgcgcg gcgaacagat
     9301 ttattggata ggcccggtag gcagggtttc cgacaaagaa gagggaacgg atttcggtga
     9361 atgcggcgca ggtttcatta ccgtaacgcc gctgcaaatc gacctgaccg cctatccgga
     9421 catggcggaa acagcggcgt tctggcatgc ggactgaccg tttcatcaaa tatagtggat
     9481 taacaaaaac cagtacggcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg
     9541 ctgaagcacc aagtgaatcg gttccgtact atttgtactg tctgcggctt cgtcgccttg
     9601 tcctgatttt tgttaatcca ctataccgtc gggctttgcg cttgagaaga acgcgcattt
     9661 ttattatgat ataccgcatt caggctatat atcggaacat gaaggttatc ttatgttgaa
     9721 acaaacgaca cttttggcgg cttgcgcctc ctttgccgca ctgttgggcg gttgtgctac
     9781 ccagcagcct gcccccgtca ttgcaggcaa ttcaggtatg caggacgcgc cgtcttcggc
     9841 agtttacaac aacccctatg gagcaacgcc gtacagcccg gctcctgccg gcgatgcgcc
     9901 ttatgtgccg ccggtgcaaa gcgcgccggt ttatacgcct cctgcttatg ttccgccgtc
     9961 tgcacctgcc gtttcgggta catacgttcc ttcttacgca cccgtcgaca tcaacgcggc
    10021 gacgcatact attgtgcgcg gcgacacggt gtacaacatt tccaaacgct accatatctc
    10081 tcaagacgat ttccgtgcgt ggaacggcat gaccgacaat acgttgagca tcggtcagat
    10141 tgttaaagtc aaaccggcag gatatgccgc accgaaagcc gcagccgtaa aaagcaggcc
    10201 cgccgtaccg gctgccgcgc aaccgcccgt acagtccgca cccgtcgaca ttaacgcggc
    10261 gacgcatact attgtgcgcg gcgacacggt gtacaacatt tccaaacgct accatatctc
    10321 tcaagacgat ttccgtgcgt ggaacggcat gaccgacaat atgttgagca tcggtcagat
    10381 tgttaaagtc aaaccggcag gatatgccgc accgaaaacc gcagccgtag aaagcaggcc
    10441 cgccgtaccg gctgccgtgc aaacccctgt gaaacccgcc gcgcaaccgc ctgtgcagtc
    10501 cgcgccgcaa cctgccgcgc ccgctgcgga aaataaagcg gttcccgcgc ccgccccgca
    10561 atctcctgcc gcttcgcctt ccggaacgcg ttcggtcggc ggcattgttt ggcagcgtcc
    10621 gacgcaaggt aaagtggttg ccgatttcgg cggcaacaac aagggtgtcg atattgcagg
    10681 aaatgcggga caacccgttt tggcggcggc tgacggcaaa gtggtttatg ccggttccgg
    10741 tttgagggga tacggaaact tggtcatcat ccagcataat tcttccttcc tgaccgcata
    10801 cgggcacaac caaaaattac tggtcggcga aggtcagcag gtcaaacgcg gtcagcaggt
    10861 tgccttgatg ggcaataccg acgcttctag aacgcagctt catttcgagg tgcggcaaaa
    10921 cggcaaacca gttaatccga acagctatat cgcgttctga ctttctgcct gaaatcaagt
    10981 tggaaatgcc gtctgaaagg ttttcagacg gcatttttgt ttttggcggc tgtgttttca
    11041 aatcattcgg aagggacggg gcatataaat ttgtttgact gtgtttgggt atttgcttga
    11101 tttcatccgt ttgcagtaga aacggatggc gcgatgtgcc tgtctttcag acggcatagt
    11161 ccgccgtcag cagccgcagg acatgtcttc agaggcttgc aggctgatgt ctctgcgttt
    11221 tttggctttt tcgtagcggc agcgttggcg gtcggtcagg atttccagcg gagggacagg
    11281 gacgggtttg ccgtctttga ccgcaaccat cgtgaagtag caactgttgg tatggcggac
    11341 ttcgccggtg cggatgtttt gtgcttcgac acggatgccg atttccatcg aggtgcgtcc
    11401 cgtgtagttg acagcggcgt aaaaggtaac caaatcgccg atgtggatgg gttctttaaa
    11461 caggactttg tcaaccgaca gggtaacgca ataattgccg ctgtaacggc tggcgcagga
    11521 ataggcgact tggtcgagca ggagcaggag ttcgccgccg tgtacgttgc cgctgaaatt
    11581 ggcggtgtcc ggcatcatca gttcggacat aatgagttcg tgcgaaggca gttggcgttg
    11641 ttgtgtcatg gtgtacctgt tttaagaaag ggtgttttga aatgggtgcg gggaaatttt
    11701 agggaatggg gaagtggaat tcaagctaaa aaaagctatg atgggatagt ctgccgaaat
    11761 ggaccgccga tgccgtctga agccttcaga cggcattttg tttaggaaag ccaaaaacgg
    11821 aagaccagga aaaacacgag tgccaatacg gcgacccaaa cgacgagcat gaatccggca
    11881 actgcccgtc ccatacggta aaccgggttt ttgcgttcgt cttcgatggg ggaggtgtgg
    11941 tgtttttcgg gttgtccgga cattttgttc tcaatcggaa aagttggaaa cgggcgtaag
    12001 gttatgttgc cgtgtaaaaa gattcaagaa ggaaggtcag cagccgtctt cccaattttc
    12061 gtcccaagcg tgtttcaatg ccgtctgaac ggtatcggca tcgaatccgc gataggcgag
    12121 gaaacgcgcc tgtttttgtt tttctttgag gtcggcggcg gggtgtttga attttttacg
    12181 caacacggct atggcggcca gtttttcgct tgagcggtcg ggaagcaggt tgcggctggt
    12241 ttcttcatct atgccctgtt gcgccaaagc ctgtttcagc ctcaatgaac cgtgtttgcg
    12301 gcttttgctg cggatatagg cttcggcata gcgcaaatcc gactgccagt tgcgttcggc
    12361 aaattcgttt aacacgtttt ccaactcctc ttcgctttcg gcgtgcggtg caagtttgcg
    12421 tttcagaccg atgcggctga gttcttggcg cgagaggatg tccatcgcgc gggcgcgtag
    12481 ggatttttgc ggtttcatgg cggcggtttc ggcgtggggc aggtttcaga cggcatagcg
    12541 gcgcaaaccg gagccgtccg aatgcctact cttctttgtt tttctgaggt tgcaggtttt
    12601 cgtaaatttc gggcagcgag ccgataatcc agtcggggcg ggtcgcatcg tcttgcgaga
    12661 gcagcgtcat atcgccgtaa ccgaaggtaa cgccgacgct caggcagccg gcggctttgg
    12721 cggcgatgat gtcgttgcgc gagtcgccga ccataaccat gtttgcaaca tcgataccca
    12781 aaacttcggc ggcgtgccgc agcggcaggg ggctgggttt tttctcgggc aggctgtcgc
    12841 cgccgagtat caggctgaag tagtcggcaa gtccgagctg tttcaacagt tcgacggcaa
    12901 ggatttcgtt tttgttggta atgactgcca gcgggatgcc caaagatttg agcaatgcca
    12961 gcccggcttc ggtttcggga taggggcggg tgaagacgct caaatggtcg cggtagtatt
    13021 tcatatagga tacgaaacct ttttcccaca gttcggaatc ggcttcgcgg tcgcggtcgt
    13081 tggtgaggac gcggtgaacc agtttgccga tgccgtcgcc cacatagctt tcgaccactt
    13141 tggcgggcag cggtttcata ccgagttgtt ccaacatcgc ttctgcggcg gcggcaaggt
    13201 cggggacgga atcgcacagt gtgccgtcca aatcgaaggc gacggcttgg acgtgttcga
    13261 tggctgcatt cataatctga tttcctgtcg ggttgaaagg gtttatttta acatcgtttg
    13321 cacagggctt ctaatgtata gattgttaac aatcgcaaca ggttttgcag gcggtgtttt
    13381 cggatttcgg ggcggaatcg ggcaagggtt tgaaatccca gcgatgttcc aattaaattt
    13441 ttaaaaaata aaaggatgcc tatatcttta tttaatttga catttatttt tcggacgcgc
    13501 gcgctgcttt ttcaggcgaa gtccgggctg attccgtgcg gcttgaaaaa taaaatcata
    13561 tatagattaa tcaaatcggg cttatgccga ctacacgcta ttacattttg tcgggaaatt
    13621 ggttatagta atgagcgaaa tgcacgcccc tgaaaatctt tttgtgaaaa ggaagcaaaa
    13681 tgtctgaagc ccttgcaaaa gaaaccctca atccgtttga aatcgcgcgc aaacaggtca
    13741 aaaccgcctg cgaccggcta aaaaccgatc cggccgttta tgaaatcctg aagagtccga
    13801 cccgcgtgtt ggaagtcaat tttcccgtca aactcgacga cgacacggtc aaaaccttca
    13861 cgggctaccg ttcgcaacac aacaacgccg tcggccccta taaaggcggc gtgcgcttcc
    13921 atcccggagt gaatctggac gaagtcaaag ccctgtcgat ttggatgacc atcaaatgct
    13981 gcgtagcggg cattccttac ggcggcggca aaggcggcgt taccttggat ccgcgcgatt
    14041 attccgaggc ggaactggaa cgcatcgccc gcgcttattc cgaagccatt tctccgctga
    14101 tcggcgagaa aatcgatatt cccgcacctg atgtgaacac caacggcaaa atcatgtctt
    14161 ggatggttga tgcctatgaa aacgtggtga aacattctgc accgggcgta tttaccggta
    14221 aaccggttga gttcggcggc tctttggcgc gtaccgaggc taccggttac ggcgtgaacc
    14281 tcgccgccgt ccaagccttg gaaaaactgg gcaaagacgt gaagggtgcc acttacgcca
    14341 tccaaggctt cggtaatgtc ggttaccaca caggttatta cgcgcatcaa tccggcgcga
    14401 aagttgttgc ggtttctact gttgacgttg ccatttacaa cgaaaacggc ttggatatgg
    14461 aagcaatcat caaagaattc caaaccaaag gtttcatcac caatgaagcc ggctacggta
    14521 aagaaatcag caatgccgaa cttttggctc tggaagtgga cgtacttgcc ccttgcgcac
    14581 tggaaaacca actgacttct gaaaatgccg gtaaagtccg cgcgacaatc gtggtcgaag
    14641 gcgcgaacgg cccgactacg cccgaagccg acgttatcct gcgtcaaaac ggcgtattgg
    14701 tcgtgcccga tattttggcg aactgcggcg gcgtggtggt atcttatttt gaatgggtac
    14761 aaaatctgca aggctattat tgggagtttg acgaagttca ggaaaaagaa accgtcgtcc
    14821 tgcgccgtgc gttccgcgat atatggaact tggcgcaaga gtatgatgtc gatttgcgaa
    14881 ccgcgtctta catgatgagt atccgccgtg ttgaaaaagc gatgaaactg cgcggttggt
    14941 attgatgttt gaacaaaaaa tgccgtctga agccttcaga cggcattttt gtgcgggcgg
    15001 gattaatggt gcgaatggcc gatttggaag gttaaagtcg agtagttcgc ctgtttttgg
    15061 cacacgcttt gatcggggaa gtcggctttg tgttcgacat tggctttcca gaagccttgg
    15121 cgcaagggga tgatgtccac ttcgcctttg tcgtctgtgc tgtcggagaa agcctgtgct
    15181 tcggttttgt gcgttttgct gcggtcgctg gtgtcgaagc cgtcaaaggt ggcggtaacg
    15241 gtggcattgg gcagcggttc gccacggaac agaacgcgga ctttgaagcg ttcgcctacg
    15301 tgaatgttgg cgggattgtc cagcgggacg atttccaagt tttgtccgac cggtttggtg
    15361 atgatggcgg tgtccgcgct ttcgtgtccg acgttgacga tgtttttgcc gaacattcgg
    15421 gtttgttcgc aatagcttgc gtcaggcatt tctttgatgc ccgcctgttt ccagcctgct
    15481 ttgttttttg accagaaagt aggctgatat tcggcgatga cgaggtaact gccgtcctta
    15541 acgggacggt tgcttcggta ctggtagttg tatgtgccgc gttgaatcat gttttccttg
    15601 cctttttcgg taaccagctg catcggtttg ctgaagatgt gcaggcggtc tttggcgatg
    15661 ggttcgagtt cgggaaattc gccgtagccc aagtcggctt taaggtattc gccgccgtgc
    15721 gtgtgggcgg tttcgaccca gacgcggtgg gcgtgggcgg atgtggcaaa tagggcggaa
    15781 acggcgagca gtgtcaatgt tttcttcatg gtgcagcctt tgtctcgtgt gggtcaaaga
    15841 ttttgttatg ttataacaaa ataggtatcg tgtgaagacg gatttgctgt ctgaaaactg
    15901 tctttttgta tagtggatta acaaaaacca gtacggcgtt gcctcgcctt agctcaaaga
    15961 gaacgattct ctaaggtgct gaagcaccaa gtgaatcggt tccgtactat ttgtactgta
    16021 tgcggcttcg tcgccttgtc ctgatttttg ttaatccgct atatcgattt gaatttttca
    16081 gaaaatgaag cggacgtttg ggcggaggca acttgtttga taagatagca atatttcaaa
    16141 acggagaaag atcatgcctt atgtcaatat taaagtaacc ggcggcaagg aagcaccgac
    16201 tgccgcgcaa aaagcggaac tgatcggcgg cgtaaccgaa ttgctggcac gcgtgctggg
    16261 caaaaatccc gaaacaacgg ttgtcgtgat tgatgaagtg gataccgata actggggaat
    16321 aggcggcaaa agcgtcagcg aacggcgcaa agagggcagg taaaaagcct gaaaatctcg
    16381 gtttgatgct ttaaattccg cgtgaaaaag agtacattcc cacccattgc ccaaaattta
    16441 cggaacacat catggataaa tttcccaagt ctgcaaagct cgatcacgtc tgttacgaca
    16501 tacgcgggcc ggttcacaaa aaagcccttc agttggaaga ggagggcaat aaaatcctta
    16561 aactcaatat cggcaaccct gcgccgttcg gctttgaagc ccctgatgaa atcttggtcg
    16621 atgtcatccg caacctgccg acttcgcaag gctattgcga ttctaaaggg ctgtattccg
    16681 cccgcaaagc cattgttcac tactatcaga ccaaaggttt gcgcgatatt acggttgatg
    16741 atgtctatat cggcaacggc gtgtccgagc tgattacgat gtctatgcag gcattgctca
    16801 acgacggcga cgaaatcctg attcccgcgc ccgactaccc tttgtggacg gcggcggcaa
    16861 cgcttgcggg cggtacggta cgccattatc tgtgcgacga agaaaacggc tggttcccca
    16921 accttgccga tatggaagcc aaaatcacgc ccaaaaccaa agccatcgtc gtcatcaatc
    16981 ccaataatcc gacaggcgcg gtgtacagca gggaaatcct gttggaaatc gccgaactgg
    17041 cgcgcaagca cggtttgatt attttcgccg acgagattta cgataaaatc ctttatgacg
    17101 gcgcggttca ccaccacatc gccgcgcttg cccccgacct tttgacggta acgttcaacg
    17161 gtttgtccaa atcctaccgc gtcgccggct tccgccaagg ctggatggtg ctcaacgggc
    17221 cgaaacatca cgcaaaaggt tacatcgagg gtttggatat gctctcgtct atgcgcctgt
    17281 gtgccaatac gccgatgcag cacgcgattc agacggcatt gggcggctat cagagcatca
    17341 acgaatttat tttgcccggc gggcggcttt tggaacagcg caacagggcg tgggaactgg
    17401 tcagccaaat ccccggcgtg tcctgcgtca aaccgatggg cgcgatgtat atgttcccaa
    17461 aaatcgatac cgaaatgtac cgtatccacg atgacatgaa attcgtttac gatttgctgg
    17521 tgcgcgaaaa agtcttgctg gtacagggaa cggggtttaa ttggattaaa cctgaccatt
    17581 tccgcattgt cacgctgcct tacgtccatc agattgaaga ggcgatgggc aggttggcaa
    17641 gattcctgca aacctaccgc caataagggg acggtttgtc tgccgaggat aaaaaatgcc
    17701 gtctgaaacg gagattcccg tttcagacgg cattttcaac agcaggaacg aatcaggcaa
    17761 atttcagtct gtcgccgtcg gcttctaccc tgatttcgct ttcgggcgca tagtttccgg
    17821 caagcagggc ttttgccagc gggttttcga tttccgactg gatggcgcgt ttgagcggac
    17881 gtgcgccgta aatcgggtcg aaaccggctt tggcgatgat gtccagtgcg gcatcggaaa
    17941 cagtcaggcg caggttttgt ttttccaaac gtttttccaa acctttgagc tggattttcg
    18001 caatgctgcg gatgtttgcc tgatccaaac cgtggaacac gaccacttcg tcgatgcggt
    18061 tgatcatttc aggacggaaa tgttctttca catcctccat cacaacttct tttaccgctt
    18121 cgtaatcctg aatgcccatt tgttggatgt gctggctgcc gatgttggaa gtcatcacaa
    18181 tcacggtatt tttaaaatct accgtcctgc cttgcccgtc ggtcaaacga ccgtcatcca
    18241 atacttgcag caggatgttg aacacatcgg gatgggcttt ttccacttcg tccagcagaa
    18301 tcacgctgta cggtttgcgg cgcacttgtt cggtcaggta gccgccttct tcgtagccga
    18361 catagcccgg aggcgcaccg atcaggcgtg caacggcgtg tttttccata tattcggaca
    18421 tatcgatgcg aatcagatga tcttcgctgt cgaacagaaa gcctgccagg gctttacaca
    18481 actcggtttt acccacgccg gtcgggccca agaacaggaa gctgccgtaa ggcttgttcg
    18541 gatcggcaag accggagcgg ctgcggcgga tagcgtcgga cacggcacgc acggcttcgt
    18601 cctgtccgac cacgcggcgg tgcaatactt cttccatttt cagcagttta tcgcgttcgc
    18661 cttccatcat tttggatacg ggaatgccgg tcatacggga aaccacctct gcgatttcct
    18721 ctgcgccgac attattacgc aagagtttgt ttgccggttt tgtgctgtcc gtatctgccc
    18781 gttcggcggc tgcacgctgt ttttccaaat gctccaaatc ttcatacatc aattttgaag
    18841 ccagtgccaa atcgccttgc cgttttgcct gttcgatttt aattttgact tcgtcaattt
    18901 gtttcttaat attagcagca ccgtctgaaa ttgctttttc ggctttccag atttcgtcta
    18961 aatcggcgta ttctttttgc agaccgttga tttcctcgtc tatcagttcc aaacgttttt
    19021 tgctggcatc gtctttttct ttttcaacgt gcgccttttc catccgaagc tgaattagac
    19081 ggcggtcgat tttgtccatt gcttccggct tggtttcttt ttccatcttg acacggctgg
    19141 cggcttcgtc aatcaaatca atcgctttat cgggcaggaa gcggtcggtg atgtagcggt
    19201 cgctcaactc cgctgcggca acgatagcag ggtcggtaat atcgataccg tggtggattt
    19261 cataacgctc ctgcaaaccg cgcaagatgg cgatggtgtc ttccacgctg ggctcgccaa
    19321 ccaatacttt ttggaaacgg cgttcgagtg ccgcgtcttt ttcgatgtat tggcggtatt
    19381 cgtccaaggt ggtcgcaccg atacagtgca gttcgccgcg tgccaaagcc ggtttcaaca
    19441 tattgcccgc gtccatcgcg ccgtcggttt tacctgcgcc aaccaaggta tgaatttcgt
    19501 cgatgaaaat cagagtgttg ccatcgtctt tcgccaagtc gttcaacacg cctttcaagc
    19561 gttcttcaaa ttcgccgcga tatttcgcgc cggcaatcaa agccgccaag tccagaacca
    19621 gcaggcgttt gttgcgcagg gattcaggca cttcgccgtt gacgatacgt tgcgccaagc
    19681 cttcaacaat agcggtttta cccacacccg gctcaccaat cagcacgggg ttgtttttgg
    19741 tacggcgttg caatacctga atcgcgcggc ggatttcatc atcacggccg ataacggggt
    19801 caagtttgcc gtcgcgggcg cgctgggtca gatcaagtgt gtattttttc aaagcatcgc
    19861 gttggtcttc ggcattcgca tcattcacgt tttgtcctcc tcgtaccgca tcaatcgcgg
    19921 cattaatgtt ttgttcggtc gcaccggctt ctttcaaaat tttgccggtt gcatcgttct
    19981 gctgcaccaa ggcaagcagg aaaagttcgc tggcgatata gacatcgccg cgcttggttg
    20041 cggctttgtc catcaagttc aatacagctt gcagctcacg gctaggcata atatcgccgc
    20101 cctggccgga cactttcggc aggctgctca aatgctgctg caaacgctgt ttcacctgcg
    20161 gcacgttcac gcccgaatgc gccagcaacg cggcggcacc gctgttttgg tcgtcaagca
    20221 gggcttttaa cacaaagcct gcttccagat agctgccgtc cgcagccaac gccaaactct
    20281 gagcttctgc aagggcttgt tggaatttgg cggttaattt gtcgtatcgc atttttgttt
    20341 ccttttcaaa atgtccgctg tcgaagccta tatgtgcata attgtggata actcaagttc
    20401 tgttttctgt ttttctatat ttaattcgat atatcattga atttaaagta tataaaaatg
    20461 tataataatg tgtataacta tatcttctta atatggaaaa gtctgttgtc ggctggatgt
    20521 aggtggcaaa tcgggtataa tcggcacatc tttttccctt tcagacggca ttgatgccgc
    20581 aaggacattt tatgagcaaa aaacgagttc tgaccggcgt aaccaccacc ggcatcccgc
    20641 atctgggcaa ctacgtcggt gctatccgcc ccgccgtccg cgcggcgcaa aaccctgata
    20701 ccgaatcctt cctcttcctc gccgattacc acggtatcat caaatgccac gagccggaga
    20761 tgattcacca atccacccaa gccgttgccg ccacttggct tgcctgcgga ctcgaccccg
    20821 agcgcaccac cttctaccgc caaagcgaca ttcccgaagt gatggaattg aactggattc
    20881 tgacctgcat caccgccaag ggtttgatga accgagccca cgcctacaaa gccgccgtgc
    20941 aggcaaattc agaaaacaat caggaagatc ccgatttcgg cgtggaaatg ggtttgttca
    21001 gctatccgat tctgatgacc gccgacatcc ttatgttcaa cgccaacgaa gtgcccgtcg
    21061 ggcgcgacca aatccaacac gtcgaaatgg cgcgcgacat cgccggccgc ttcaaccacc
    21121 gctttaaaga aatctttacc ctgcccgaag tgaaaatcga tgaaaacgtc gaactcttgg
    21181 tcggtttgga cggacgcaaa atgtccaaat cctacggcaa caccattccg ctttgggaaa
    21241 acgacaaaaa aacccaaaaa tcggtcaaca aaatcatcac caatatgaaa gagccgggcg
    21301 agccgaaaca gcccgacgaa agcccattgt ttgaaatcta caaagccttc tccacgccgt
    21361 ctgaaaccgc agcgttcacg caaatgcttg ccgaaggctt ggcttggggc gaggcaaaaa
    21421 aacttttggc ggcgaaaatc aacgccgaac tcgccgaacc gcgcgaacgc tacaacgagc
    21481 tgaccgccga cccttcgcaa atcgaagaga ttttgcaggc aggcgcggcg aaagcgcgta
    21541 aagaagcacg cgaattgttg gacaaagtac gcgatgcggt cggcatccgc ccgttgaaat
    21601 aaatccatgc cgtctgaaca tccattgtcc gtgtttcaga cggcattttg aaaccatcag
    21661 gagtgtggaa tgctgaaaac atcttttgcc gtattgggcg gctgcctgct gcttgccgcc
    21721 tgcggcaaat ccgaaaatac ggcggaacag ccgcaaaacg cggtacaaag cgcgccgaaa
    21781 ccggttttca aagtcaaata tatcgacaat acggcgattg ccggtttgga tttgggacaa
    21841 agcagcgaag gcaaaaccaa cgacggcaaa aaacaaatca gttatccgat taaaggcttg
    21901 ccggaacaaa acgtcgtccg gctgaccggc aaacatacca acgacttgga agccgtcgtc
    21961 ggcaaatgta tggaaaccga cggaaagggc gcgccttcgg gctgggcggc aaacggcgtg
    22021 tgccatacct tgtttgccaa actggtgggc aatatcgccg aagacggcgg caaactgacg
    22081 gattacctga tttcgcattc cgccctgcaa ccctatcagg caggcaaaag cggctatgcc
    22141 gccgtgcaga acggacgcta tgtgctggaa atcgacagcg aaggggcgtt ttatttccgc
    22201 cgccgccatt attgaggtat tcggacatcc cggaatatat ttgggttttt caaaccctgc
    22261 aggaaaaagt ccgcaccgtc gggaaactca aaaggagggg gatatgtgtt acaattttcc
    22321 gaactgtttt cataaaatag ttttcggacg tgtttcaata tggcattgat gccgccttat
    22381 tgttcgagaa aaaaccttta tatttaaata taatgggttt taactaaacg ggaaaccgtt
    22441 ttctctccgg ccgatgggca aaatcagccg attgatggaa cacggtccga tttttaagca
    22501 aaaccttaat caacaactca agaaggataa gacatgaaaa aattattgat tgccgcaatg
    22561 atggcggctg ccttggcagc ttgttcgcaa gaagccaaac aggaggttaa ggaagcggtt
    22621 caagccgttg agtccgatgt taaagacact gcggcttctg ccgccgagtc tgccgcttct
    22681 gccgtcgaag aagcgaaaga ccaagtcaaa gatgctgcgg ctgatgcaaa ggcaagtgcc
    22741 gaggaagctg taactgaagc caaagaagct gtaactgaag cagctaaaga tactttgaac
    22801 aaagctgccg acgcgactca ggaagcggca gacaaaatga aagatgccgc caaataattt
    22861 gttgccttgg caaaaatgat gggatgccgc ctgccggcaa cccaaacgaa accgcctgaa
    22921 gattttcagg cggtttttga gttgtatgcc ccttgttttt tacgcctgca tgaccgttcc
    22981 gagcagatgt gccgcgcata tcgggatgtt gcggacaggc aaatgccgtc tggacagggt
    23041 ttggacggca tttgttccgt aaggttcaga cgacctcgac tttaaacggc atattgattt
    23101 tttgccattg gacgctttca tattccaatg cgtcggcaat caggggatgg cgttccagcc
    23161 attccctgtc aatacgcagg atgaagccgc agctttccgt atccgtgcgc aactgcatat
    23221 ttttcgggaa agacaggtct tggcgcgaac ggcagaacag ggcagcaagg cgcagggaca
    23281 aaacggcata ccacaacatt tcgttggtgc cgatgatgcc gctcattttt ttcatatcgc
    23341 cgcgatgacc gatgaccagt tgggcaagta tggtctgttc tttgcgtgag aaacccggca
    23401 tatcggcgtt ttcgaggatg taggcggaat gcttgtgata gccggtgtgg gcgatgtcca
    23461 aaccgatttc gtgcagcgcg gcggcgcgtc cgagatactg ttgccacaag gcaagctctt
    23521 gaactgtaac gtttttagcg tggcagaggc tgtccataaa ggtttgcgcg gtctcggcgg
    23581 tgcgtttcgc ctgattgagg ctgacgtggt agcggtgttg gaactcggca accgtttgtc
    23641 cgcgcatatc ttcgtttaaa ccgcgcccga tcaaatcgta aaacacgccg tcgcgcaggg
    23701 cggcttcggt tacggtcata ctgtcgagtt tcatttcctc aaacgccgcc atcatcacgg
    23761 caagtccgcc ggcaaaaact tcgatgcgtt ccggtttcag gttttcaaat ttggcttttt
    23821 tgaccgaacc ggcttcgatg atgcgttcgg caagggcgcg catacctttg taggtaatgt
    23881 ccgcctcttg gggcatttcg gcggcaagca cgtcgcggat ggattttgcc gaacccgatg
    23941 tgccgacggc gaaatcccaa ccttcgcgcc tcatattttt gctgatgcgc tggatttcgt
    24001 tgcgggcggc ggaaatggca gattggaagt ctttggcggt gattttgttt tggaagaagc
    24061 gcaggctgta ggttacgcag cccaagggca ggctttcggt aatgtcggga ttcagcgtcg
    24121 agccgatgac aaattctgtc gaaccgcccc cgatgtcgat aaccagcatt ttgccgccgc
    24181 ccggggggag ggtgtggatc acgccggtat aaatcagccg cgcctcttcg cgccctgcga
    24241 tgatttcgat ggggaaaccc agtgccgctt cggccttggg aaggaaatct gcgatgtttt
    24301 tggcgacgcg gaatgtattg gttgccacgg cgcgtacctg ttcagggcgg aagccgcgca
    24361 ggcgttcgcc gaattttgct aggcagtcta aagcctgttc ctgtgaggat gcgctgaggt
    24421 ttttctgttc gtccagaccg gcggcgaagc gcaccatctg tttgaaggaa tcgataactt
    24481 ttaattgtcc gttgttgttt tcgcaaatct ggaggcggaa actgttggaa cccaaatcga
    24541 cggaggcgag gatgtttgcg ggggtggtgg tcatggcgga tatcggtggg ggaaaaacgc
    24601 aatgttactc tgacggcgca ggcgttgaca ataaatgatg cggcggtttt tgattctgcc
    24661 cacggatgtt gccgacggca tttttgcgct tatttgaaat ccttttccac gctcatgaaa
    24721 atctgcgtgt ttttgcgtgt gtaaaaactt ttcatattgc tgtcgatttt cagatagcgg
    24781 aaattgagtt gcggcgtaaa gcccttccaa gagattttgt cgtgccacaa cgacaggttt
    24841 gcctgatatt cgtggtcttt gcgcgggaag cggtacacaa tggtcccggg tgcgtcaaac
    24901 atcctgcggg tatagcgcag gtttgcccgc agacccaagc ccgccgtcga acgttttgac
    24961 tgcgccgaca cgcaaaccct tgcggacgga agcctgttcc gcctctttcg tcatgttgtg
    25021 cgaccagtcc gcaccgccgt aaagcagcca gtctttcggc gcggaataca tcagcgtcgc
    25081 gcccgccagc ggcatatggc tgtcgtatcg ggcggcggtg cggtcttcct gataacgctt
    25141 ccacatattg cccacgttca gcgtcagccg ccagcgttcg ctcaagcgtc gggagaaatc
    25201 ggcattgaag ccgccgacga aattgtatcg gctgccgcct aagaggtttt gcccgataaa
    25261 cggcacgatg ccgaacgagc gcgttaccga acgattttta tagccgagcg acaggcgcag
    25321 gcactgttcg ctgaaatctt tgttatccca ataatgcacg ccgccgccgc tgatgccgcc
    25381 gtagaggaaa tgatgcccgc ccgcattgat ttcgcgcgac acgcctaagc cctagcgcaa
    25441 accgtgtgcc ttttgcggca ggctgtcggc ggttttcgtc cagcttctgc ccgcaaattc
    25501 aatcgttttt ttggacgaag cgtt
//
LOCUS       DAWFHY010000021        25478 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.021, whole genome shotgun sequence.
ACCESSION   DAWFHY010000021 DAWFHY010000000
VERSION     DAWFHY010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 25478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25478
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.021"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..410)
                     /locus_tag="WGQ68_000780"
     CDS             complement(<1..410)
                     /locus_tag="WGQ68_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714621.1"
                     /translation="MKTDIQTELTQALLPHEKVWANEEKTILAKNILLDLVEKTDPSI
                     IELLLGNDELKRHFFVEVNGVLVFKLQDFRFFLDKHSVNNSYTKYANRIGLTDGNRFL
                     KDNSDIVLDFPFKDCVLNGGQSTEEGEEIYFKRN"
     gene            complement(614..1537)
                     /gene="truB"
                     /locus_tag="WGQ68_000781"
     CDS             complement(614..1537)
                     /gene="truB"
                     /locus_tag="WGQ68_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEZ0714622.1"
                     /translation="MNTGKPQKRAVNGVLLLDKPEGLSSNTALQKARRLFRAEKAGHT
                     GVLDPLATGLLPVCFGEATKFSQYLLDADKAYTATLKLGEASSTGDAEGEIVATARAD
                     ISLAEFQTACQALTGNIRQVPPMFSALKHEGKPLYEYARKGIVIERKARDITVYAIDI
                     AEFDAPKAVISVRCSKGTYIRTLSEDIAKHIGTFAHLTALRRTETAGFTIAQSHTLEA
                     LANLDETGRDSLLLPCDVLVAHFPQTVLNDYAVHMLQCGQRPRFDEDLTSDTPVRVYT
                     ENGHFVGLAEYQKEICRLKALRLMNTAASAV"
     gene            complement(1570..1941)
                     /gene="rbfA"
                     /locus_tag="WGQ68_000782"
     CDS             complement(1570..1941)
                     /gene="rbfA"
                     /locus_tag="WGQ68_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEZ0714623.1"
                     /translation="MRKPQRGYARQDRVKEQIMRELAELVRTGLKDPRAGFITVNEVE
                     VTRDYSHATVFYTILNQDAREITEEVLEHARGHLRSELAKRIKLFKIPELHFKYDESL
                     ERGLNLSALIDQVAAEKPVED"
     gene            2132..3376
                     /gene="clpX"
                     /locus_tag="WGQ68_000783"
     CDS             2132..3376
                     /gene="clpX"
                     /locus_tag="WGQ68_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEZ0714624.1"
                     /translation="MSNENRTCSFCGKSKSHVKHLIEGENAFICDECVSNCIEILHED
                     GNDGTPSESAGGEPEESGKLPTPAEIVANLNDHVIGQEQAKKALAVSVYNHYKRLRHP
                     KAGANVELSKSNILLIGPTGSGKTLLAQSLARKLDVPFVMADATTLTEAGYVGEDVEQ
                     IITKLLGKCDFDVEKAQRGIVYIDEIDKISRKGDNPSITRDVSGEGVQQALLKLIEGT
                     VASVPPQGGRKHPNQEFINVDTTNILFICGGAFAGLEKVIRQRTEKGGIGFGASVHSK
                     DENADITKLFGIVEPEDLIKFGLIPELIGRLPVIATLEELDEDALINILTEPKNALVK
                     QYQALFGMENVELEFEEGALRSIARQAMERKTGARGLRSIVERCLLDTMYRLPDLQGL
                     KKVVVGKAVIEEGREPELVFES"
     gene            complement(3441..4634)
                     /locus_tag="WGQ68_000784"
     CDS             complement(3441..4634)
                     /locus_tag="WGQ68_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEZ0714625.1"
                     /translation="MQNYLTPNFAFAPMIPERASGSRVWDTEGREYIDFSGGIAVNAL
                     GHCHPALVDALNAQMHKLWHISNIYTTRPAQELAQKLVANSFADKVFFCNSGAEANEA
                     ALKLARKYARDRFGGGKSEIVACINSFHGRTLFTVSVGGQPKYSKDYAPLPQGITHVP
                     FNDIAALEAAVGEQTCAVIIEPIQGESGILPATAEYLQTARRLCDRHNALLILDEVQT
                     GMGHTGRLFAYEHYGIVPDILSSAKALGCGFPIGAMLATEKIAAAFQPGTHGSTFGGN
                     PMACAVGSRAFDIINAPETLHNVRSQGQKLQTALLDLGRKTGLFSQVRGMGLLLGCVL
                     DAPYRGRASEITAAALKHGVMILVAGADVLRFAPSLLLDDEDTAEGLRRLEHVLTEFA
                     AANRP"
     gene            5038..5538
                     /locus_tag="WGQ68_000785"
     CDS             5038..5538
                     /locus_tag="WGQ68_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714626.1"
                     /translation="MNAMPQTAAQNETLHTCSIIVDDAQDFSPMLLDAGRDDTLISES
                     MIPQIRTVAALIAAERHDFSRSSPAEFTDAADFFAARILVLGVRRFHLDVSLLQILKT
                     ANSRARRFAEKHRLFFTPAQAELSLNKHKNRRLLTVETEHEVENKGNPVANSLAFVRK
                     LHTLPL"
     gene            5623..6177
                     /locus_tag="WGQ68_000786"
     CDS             5623..6177
                     /locus_tag="WGQ68_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714627.1"
                     /translation="MKKIIASALIATFALAACQDDTQARLEQQQKQIEALQQQLAQQA
                     DDTVYQLTPEAVKDTIPAEAQANGNNGQPVTGKDGQQYIYDQSTGSWLLQSLVGAAAG
                     AFIGNALANKFTRAGNQDSPVARRARAAYHQSAHPNARTSRDLNTRSLRAKQQAAQAQ
                     RYRPTTRPPANYRRPAMRGFGRRR"
     gene            6191..6361
                     /locus_tag="WGQ68_000787"
     CDS             6191..6361
                     /locus_tag="WGQ68_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009348581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714628.1"
                     /translation="MPSEGLSDGIFAFVRGIVMLPFDFQTAFCFPAFDVGMAILIYVR
                     WQGGIVMPPLGF"
     gene            6501..6668
                     /locus_tag="WGQ68_000788"
     CDS             6501..6668
                     /locus_tag="WGQ68_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714629.1"
                     /translation="MNLRKFLIVAKAEKGFYYSLILWVSKPGGAETLQKCRLKLEIGF
                     RRHWFMRQVGA"
     gene            complement(6665..8053)
                     /locus_tag="WGQ68_000789"
     CDS             complement(6665..8053)
                     /locus_tag="WGQ68_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0714630.1"
                     /translation="MSIKFADLNLDKNILSAVSSEGYESPTPIQAQAIPFALEGRDIM
                     ASAQTGSGKTAAFLLPTLQKLTKRSEKPGKGPRALVLTPTRELAAQVEKNALAYAKNM
                     RWFRTVSIVGGASFGYQTRALSKPVDLIVATPGRLMDLMQSGKVDFERLEVLILDEAD
                     RMLDMGFIDDIETIVEATPSDRQTLLFSATWDGAVGKLARKLTKDPEVIEVERVDDQG
                     KIEEQLLYCDDMRHKNRLLDHILRDANIDQCVIFTSTKAMTEVIADELYEKGFAANCL
                     HGDMPQGWRNRTLMDLRKGRCKILVATDVAARGIDVPTITHVINYDLPKQAEDYVHRI
                     GRTGRAGRTGIAITFAEVNEYVKVHKIEKYINRKLPELTIEGMEPTRKRKSAGGKPKG
                     KGGWGDRKSGGRRGDHKPGKEGFGGKTRGEGFKKEGFKKDGFKKTGEGFKGKRKAGDS
                     FAGRGERRYKDR"
     gene            8381..9328
                     /locus_tag="WGQ68_000790"
     CDS             8381..9328
                     /locus_tag="WGQ68_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0714631.1"
                     /translation="MTDITPFANRLGKNIKHLMKWAKRNGIEAWRIYDRDIPQFPFAA
                     DVYGDRIHLQEYDTGWLMQPEEYEAWLAEVLEAVVFVTGFAPEQIHLKRRERQKGLQQ
                     YEKTGKTGDDFVITENGRKFWVNLDKYLDTGLFLDHRNTRKKVGETAAGKRFLNLFSY
                     TGSFTVYAATGGAASSETVDLSNTYLDWAKRNFELNGIDTERHKIVRADVFQYLQTAY
                     GEGRRFDLIVMDPPSFSNSKKMSDILDIQRDHKKLIDGAVKLLASDGILYFSNNLRSF
                     VLDDLVSEQYAVKDISKQSVPEDFRNKKIHRCWEIRHES"
     gene            complement(9381..9713)
                     /gene="trxA"
                     /locus_tag="WGQ68_000791"
     CDS             complement(9381..9713)
                     /gene="trxA"
                     /locus_tag="WGQ68_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HEZ0714632.1"
                     /translation="MSSELIVHTGDAAFEQDVLKSDLPVLLDFWAPWCGPCKMIAPIL
                     DDIAAEFEGRLKVVKINIDDNEATPSRFGVRGIPTLMVFKNGDVVATKVGALAKGQLT
                     AFVEASIA"
     gene            10011..10484
                     /locus_tag="WGQ68_000792"
     CDS             10011..10484
                     /locus_tag="WGQ68_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0714633.1"
                     /translation="MTDFSVWETAPFGATVDHILQRYHNVHRAQFEELVPLAQKVAQV
                     HADTFPAEIAGLLADMQNELLMHMMKEERMLFPMINQGVGRGAAMPIGVMMHEHEEHD
                     RAIARLKELTDNFQPPEGACGSWTRLYALAKEMVEDLNDHIHLENDILFARVLDS"
     gene            complement(10554..11375)
                     /gene="nadE"
                     /locus_tag="WGQ68_000793"
     CDS             complement(10554..11375)
                     /gene="nadE"
                     /locus_tag="WGQ68_000793"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEZ0714634.1"
                     /translation="MMRIFSTGGKMDTQAVITHIVRWLDEYAARANAKGFVVGVSGGI
                     DSAVVSALAARTGRTTLLLDMPIRQHPDQLERARLHIRNLQRQYANVSAQTVDLTDTF
                     QTFEQTVGVHQTAFDNQPLSLANARSRLRMLTLYYYGQIHGLLVTGTGNKIEDFGVGF
                     FTKYGDGGVDISPIADLTKTQVYRLAEALGVDEAIQKAPPTDGLWDTERTDEEQMGAS
                     YPELEWAMGVYGTRKPEDFEGRQREVLEIYTRLHRAMQHKINPIPVCRIPPELLG"
     gene            11572..12927
                     /locus_tag="WGQ68_000794"
     CDS             11572..12927
                     /locus_tag="WGQ68_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEZ0714635.1"
                     /translation="MSDFFHSDVLSVSELNAFAKSLLENHLAGLWIAGEVSNLTRAAS
                     GHYYFSLKDSRAQVRCAMFKGAAARLAKPLKEGDHIEVSGKISIYEARGEFQITVNEV
                     RLKGLGQLYEAYERLKAQLQAEGAFAAERKKPLPTRPQCIGIVTSLAAAALRDVVTTL
                     KRRAPEIPVIVYPTPVQGAGSELQIAQAIKTASQRAECDVLIVCRGGGSIEDLWAFNE
                     EPVVRAIEACTVPVVSGVGHETDFTLADFVADVRAPTPTGAAELVSPNRQESLHRLAQ
                     AQGHLKTILEQRYFDASQKLDWLARQIRHPRQKLDDQRTYIHKLVQTLSYSMTQNLRA
                     HTARFERQTQTLKHCRPDVSVYKHNIDRFQTALSHAFHQLLTHRRQSLTAQAALLEAV
                     SPQHILERGFSVVKNTRGQVIRNADVLKQGQKLHIIFADGETDVRVSKEQGQQDLFDC
                     I"
     gene            13760..15301
                     /locus_tag="WGQ68_000795"
     CDS             13760..15301
                     /locus_tag="WGQ68_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEZ0714636.1"
                     /translation="MKFLDREASIAKPGFNRWLVPPAALAVHLAIGQIYAYSVFNAPL
                     TKLIGITESAAGDWKLTTVGWIFSIALAMLGASAALFGTWMERVGPRKAIFAAACCFS
                     LGFFVSAFGVRTHNLFLLYLGNGVIGGVGLGLGYIGPVSTLMKWFPDKPGMATGLAIM
                     GFGGGAMLASPLSVSLMNAFSNAASVGVAETFAVLGLLYLALMMFGAFTIRVPADGWK
                     PEGYTAPKTQNKLVSSNHVNVSQAIKTPQFWLLFWVLCLNVTAGIGVLGQASVMIQEL
                     FSETSVGRQAAVGAGAAAGFVSLLSLFNMGGRFLWSSVSDKIGRKNTYTIFFVLGSLL
                     YFAVPSIGEGGSKALFIIGFCVIISMYGGGFAAIPAYLKDLFGTYQVGAIHGRILLAW
                     STAAVIGPVLVNYIRQSQIDSGIPAAQAYSVTMYIMASLLIIGLLCNLAVKSVHEKYH
                     EKDIKTAARSGNPDDETAISDAYLVGEKVSGGGISVWWRWALAVIPLAYGVVMVFVKA
                     LDLFS"
     gene            complement(15370..16419)
                     /locus_tag="WGQ68_000796"
     CDS             complement(15370..16419)
                     /locus_tag="WGQ68_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEZ0714637.1"
                     /translation="MSKQPTSKRQWRDGAAPSAKKTAKPFKSKARPKDETGKTASQPY
                     GQKASDGIKLQNTPKQRAAKAKKLVVRNPNQKIMEHARDLKERRSDLSRMEPERLQKV
                     LAASGVGSRREMEEWINNGWVTVNGKTAQLGDKVTPDDHVTVKGSIIKLKWADRLPRI
                     ILYYKQEGEIVSRDDPQGRVSIFDRLPQAASSRWVAIGRLDINTSGLLILTTSGELVQ
                     RFAHPSFEVEREYAVRVLGGLTGEQMRVLTEEGVMLEDGLAKVERIREQGGEGANKWY
                     NVVIKEGRNREVRRIFESQGLTVSRLVRVGFGPIGLPNRLKRGQFYELNPAEVANILK
                     WAEMLLPGERRRKKA"
     gene            complement(16428..16583)
                     /locus_tag="WGQ68_000797"
     CDS             complement(16428..16583)
                     /locus_tag="WGQ68_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714638.1"
                     /translation="MHKLCLATSRSDDFIRKTAASGTISNRPTEDGILSTRPAAHAAE
                     EPPYQAS"
     gene            16643..17275
                     /gene="pdxH"
                     /locus_tag="WGQ68_000798"
     CDS             16643..17275
                     /gene="pdxH"
                     /locus_tag="WGQ68_000798"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEZ0714639.1"
                     /translation="MDLHNIREDYSKRELSEGDCADNPIEQFERWLDEAVRAQVNEPT
                     AVNVAAVDGRGRPNSRMVLLKEVNSEGFVFFTNYHSRKGRSLDAHPFAAMTFFWPELE
                     RQVRVEGRVERLAEKLSDEYFESRPYQSRLGAWASAQSEVIPNKAVLVAKAAAVGLKH
                     PLHVPRPPHWGGYIVIPDLLEFWQGRPSRLHDRIQYRLLDGGWIRERLSP"
     gene            complement(17404..18414)
                     /locus_tag="WGQ68_000799"
     CDS             complement(17404..18414)
                     /locus_tag="WGQ68_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HEZ0714640.1"
                     /translation="MNHTVTLPDQTTFAANDGETVLSAAARQNLNLPHSCKNGVCGQC
                     KAELVSGDIQMGGHSEQALSEAEKAQGKILMCRTTAQSDISLNIPGYNPNALPVRTLP
                     ARIESMVFKHDVALLKLALPKAPPFAFYAGQYIDLLLPGNVSRSYSIANPPDQEGILE
                     LHIRRRENGVCSEMIFGSEPKVKEKGIVRVKGPLGSFTLQEDSGKPVILLATGTGYAP
                     IRSILLDLIRQDSSRAVHFYWGARHQDDLYALKEAQGLACRLKNACFTPVLSRPGEGW
                     QGRKGHVQDIAAQDHPDLSEYEVFACGSPAMTEQTKNLFVQQHKLPENLFFSDAFTPS
                     AS"
     gene            complement(18506..19936)
                     /gene="gatB"
                     /locus_tag="WGQ68_000800"
     CDS             complement(18506..19936)
                     /gene="gatB"
                     /locus_tag="WGQ68_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEZ0714641.1"
                     /translation="MTWETVIGLEIHVQLNTKSKIFSGASTAFGAEPNAHASVVECAL
                     PGVLPVMNREVVEKAIKLGLALDAKINRKNVFDRKNYFYPDLPKGYQISQLDLPIVEH
                     GKLEIVVGDDVKTINVTRAHMEEDAGKSVHEGLNGATGIDLNRAGTPLLEVVSEPELR
                     SAAEAVAYAKALHSLVTWLDICDGNMAEGSFRVDANVSVRPKGQAEFGTRREIKNLNS
                     FRFLEQAINYEAEAQIEILEDGGKVQQATMLFDPEKGETRVMRLKEDAQDYRYFPDPD
                     LLPVIISDAQMQKAKAEMPELPKEMAARFVADYGVSEYDARLLTASRAQAAYFEEAAK
                     ESGQGKPTANWMNGELAAALNKEGMELADSPITAPRLAALVGKIADGTLSSKLAKKAF
                     EAMWAEPEATIAEIIEKHGLQQMTDTGAVEAMVDEVLANNAKAVEQFKSGNEKALNAI
                     VGQVMKASKGKANPAQVQELIKAKLA"
     gene            complement(19979..20910)
                     /locus_tag="WGQ68_000801"
                     /pseudo
     CDS             complement(19979..20910)
                     /locus_tag="WGQ68_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213235.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
     gene            complement(20907..22352)
                     /gene="gatA"
                     /locus_tag="WGQ68_000802"
     CDS             complement(20907..22352)
                     /gene="gatA"
                     /locus_tag="WGQ68_000802"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEZ0714642.1"
                     /translation="MTQYTLKQASVLLQSKQISAVELASAYLAAIAEKNPALNGYITI
                     DQDKTLAEARAADERIAQGNASALTGVPVAYKDIFCQTGWRSACASKMLDNFISPYTA
                     TVVQNLLDEGMVTLGRTNMDEFAMGSTNENSFYGAAKNPWNPEHVPGGSSGGSAAVVA
                     ARLAPAALGSDTGGSIRQPASHCGITGIKPTYGTVSRFGMVAYASSFDQAGPMAQTAE
                     DCAILLNAMAGFDPKDSTSLEREKEDYTRDLNQPLKGLKIGLPKEYFGEGNSADVLTA
                     LQNTIDLLKAQGAELIEVSLPQTKLSIPAYYVLASAEAGTNLSRYDGVRYGHRAAQFG
                     DLEEMYGKTRAEGFGSEVKRRIMIGTYVLSHGYYDAYYLKAQKLRRLVADDFQTAFAR
                     CDLILAPTAPTAAPKIGADASPVETYLSDIYTIAVNLAGLPALTLPAGFSGGGLPVGV
                     QLVGNYFAEAKILGAAHQIQLNSDWHGKRPE"
     gene            complement(22415..22705)
                     /gene="gatC"
                     /locus_tag="WGQ68_000803"
     CDS             complement(22415..22705)
                     /gene="gatC"
                     /locus_tag="WGQ68_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEZ0714643.1"
                     /translation="MALTLADVDKIARLSRLHLTAEEKEKSLQELNDIFTMVEQMQTI
                     NTDGIEPMAHPHEAALRLREDEVTETDRAAEYQAGAPEVRNRLYIVPQVIEE"
     gene            22856..23509
                     /locus_tag="WGQ68_000804"
     CDS             22856..23509
                     /locus_tag="WGQ68_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEZ0714644.1"
                     /translation="MGLGMEIGKLIVAFLVLINPFSALSLYLDLTNGHSTKERRKVAR
                     TAAVAVFAVIAVFALIGGTLLKVLGISVGSFQVGGGILVLLIAISMMNGNDNPAKQNL
                     GAQPETGQARPARNAGAIAVVPIAIPITIGPGGISTVIIYASAAKTYSDIALIIVAGL
                     VVSAICYAILIVAGKVSRLLGATGLTILNRIMGMMLAAVSVEIIVSGLKTIFPQLAG"
     gene            23514..24857
                     /locus_tag="WGQ68_000805"
     CDS             23514..24857
                     /locus_tag="WGQ68_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEZ0714645.1"
                     /translation="MFHSVNVFTGETLYRRPAQDYAEFERRLADLKMRGGAFAQLGVT
                     ERAARLQKFADRLEAEKERFAEMVCEEVGRCLHECRAEIGKSIELIRYYARLAPELLA
                     HKTIATQASLSQVRFEPLGVVFAVMPWNYPVWQVLRFAVPAMCAGNACAVKPAPSVAR
                     VSQALFDLVSDGIPLAGAWLDEAGTLKAVEDTDAMAFTGSTHTGRILAAHAGANLKKT
                     VLELGGSNAFIVMPDADLERAAAEACYSRFRDAGQSCNAAKRIIVTEAAADRFITLFL
                     AECAKLKMGDPKHPDTTLAPLHREDLRDRVHGQVEDAVSNGAVCLTGGKIPQGRGWFY
                     PATVLDRVNPACRVWREEVFGPAALILRAENEEHAIALANDSPFGLGACIYTADTERA
                     WRFAEKIQAGSVFINRHTSSDLRLPFGGVKDSGYGRELSEFGLYEFVNVKTYWQK"
     gene            complement(24906..25340)
                     /locus_tag="WGQ68_000806"
     CDS             complement(24906..25340)
                     /locus_tag="WGQ68_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HEZ0714646.1"
                     /translation="MNFALSVITFTLASFLPVPPAGAAVFTWKDGGGNSYSDVPKQLH
                     PDQSQILNLRTRQTKPAVKPAQADAGKRTDGAAQENNPDTAEKNRQLEEEKKRIAETE
                     RQNKEENCRISKMNLKAVGNSNAKNKDDLIRKYNNAVNKYCR"
BASE COUNT         5483 a         6532 c         7242 g         6221 t
ORIGIN      
        1 ttattgcgtt taaaataaat ctcttcgcct tcttcggtgc tttgtccgcc attcaacaca
       61 caatctttaa acggaaaatc caacacaata tccgaattgt ctttcaaaaa gcggttgccg
      121 tccgtcaaac caatgcggtt ggcgtatttt gtgtaggaat tattgacgct gtgtttgtcc
      181 aagaaaaaac ggaaatcctg caatttaaac accagcacac cattcacttc cacaaagaaa
      241 tggcgtttta actcatcatt ccccaataac aactcgataa tggacgggtc ggttttttcc
      301 accaaatcca acaggatgtt tttggctaaa atggtttttt cttcgttcgc ccatactttt
      361 tcgtgtggta gtagggcttg ggttaattcg gtttgaatgt ctgtcttcat cgttataaat
      421 cctcatcagt tattttaatg tgccattctt gtgtaacggc aaatggcttt gcaaataaat
      481 tctaataaaa tcaatccttt gatgtctttg ctgtttccgg gggcgttcgc aaagttgcga
      541 taagcggggc gggtgtccga gtgtgtcggg attttgcgga aatatcaaca tattattcaa
      601 acacagctgc cgttcagacg gcggatgccg ccgtgttcat caggcgcaag gctttcagac
      661 ggcatatttc tttttgatat tccgctaggc cgacaaagtg gccgttttcc gtgtaaacgc
      721 gtaccggcgt atcggaagtc aggtcttcat cgaaacgcgg ccgttgcccg cattggagca
      781 tatggacggc ataatcgttt aaaacggttt ggggaaaatg ggcaaccaat acgtcgcagg
      841 gcagcagcaa gctgtcgcgt ccggtttcgt ccaagtttgc caaggcttcg agcgtgtggc
      901 tttgggcgat ggtaaaaccg gcggtttcgg tgcggcgcag ggcggtcagg tgggcgaacg
      961 tgccgatgtg tttggcgatg tcttcgctga gggtgcggat ataggtgcct ttgctgcaac
     1021 gcacgcttat cacggctttg ggcgcatcaa attcggcaat atcgatggcg taaacggtaa
     1081 tgtcgcgcgc tttgcgttcg atgacgatgc ctttgcgcgc gtattcgtac agcggtttac
     1141 cttcgtgttt gagcgcggaa aacattggtg gcacttggcg gatgttgcct gtcagtgctt
     1201 ggcaggccgt ctgaaattcg gctaaggaaa tatcggcacg ggcggtggca acgatttcgc
     1261 cttcggcatc acccgtgctg ctggcttcgc cgagtttcag cgtggcggtg taggctttgt
     1321 cggcatccag caggtattgg gagaacttgg tcgcttcgcc gaagcagacg ggcaaaagtc
     1381 cggttgccaa aggatcgagc acgccggtat gtccggcttt ttcggcacgg aacaaacgcc
     1441 gcgctttttg cagcgcggtg ttgctggaaa ggccttcggg tttgtccaag agcaaaacac
     1501 cgttgacggc acgtttttgg ggtttgcctg tattcatggt tcgatgttcg gacggcatgg
     1561 gcatatccgt cagtcttcaa ccggtttttc cgccgccact tgatcgataa gggcggacag
     1621 gttcaaaccg cgttccaacg attcgtcgta tttgaaatgc agctcgggga ttttgaacag
     1681 cttgatgcgt ttggccaatt cgctgcggag gtgtccgcgc gcgtgttcca gcacttcttc
     1741 cgtaatttcg cgcgcgtctt ggtttaaaat ggtgtagaac acggtggcgt ggctgtaatc
     1801 gcgggtaact tcgacttcgt tgacggtaat gaagccggcg cgcgggtctt tcagtccggt
     1861 acggacgagt tcggcaagct cgcgcataat ttgttctttg acacggtctt ggcgggcata
     1921 gccgcgttgg ggttttctca tgaatatcct cagatttcag atttggtttc agacggcatt
     1981 tttgccgccg gggcggttgc gaaagggcgt attatagcct aatcggcggg attttaaggt
     2041 aatacattga taagaacgga tatagttatt atacttggca acccgaacgg cacgtgccgt
     2101 tttctgtaac gcatacattg taaagaaagc catgtccaac gaaaaccgta cctgttcctt
     2161 ttgcggaaaa tccaaatcac acgtcaaaca tttaattgag ggcgaaaacg cctttatctg
     2221 tgacgaatgc gtctcaaact gtattgaaat attgcatgaa gacggaaatg acggcacacc
     2281 ttcggaaagt gccggagggg agccggaaga atccggcaag ctgcccacgc ccgccgaaat
     2341 cgtcgccaac ctcaacgacc acgttatcgg tcaggaacaa gccaagaaag ccttggcggt
     2401 gtcggtgtac aaccattaca aacgcctgcg ccacccgaaa gccggagcta atgtcgaatt
     2461 gtcgaaatcc aacatcctgc ttatcggccc gaccggatcg ggtaaaaccc tgctggcgca
     2521 atctttggcg cgtaaactgg atgtgccgtt cgtgatggcg gatgcaacca cgctgactga
     2581 agcaggttat gtcggcgaag atgttgagca aatcatcacc aaactgttgg gcaaatgtga
     2641 tttcgatgtt gaaaaagccc agcgcggcat cgtctatatc gacgaaatcg ataaaatttc
     2701 acgcaaaggc gacaatccgt ccatcacgcg cgacgtgtcc ggcgaaggtg tgcaacaagc
     2761 cttgctgaaa ctgattgaag gtacggtggc aagtgttccg ccccaaggcg gccgcaagca
     2821 tccgaatcag gaatttatca acgtcgatac caccaacatc ctgtttatct gcggcggtgc
     2881 gtttgcaggc ttggaaaaag tcatccgcca gcgcaccgag aaaggcggta tcggtttcgg
     2941 tgcgtccgtt cacagcaagg acgaaaatgc cgacattacc aagctgttcg gcatcgtcga
     3001 accggaagat ttaatcaaat tcggtctgat tccggaatta atcggacgtt tgcccgtgat
     3061 tgcaacacta gaagaactgg atgaggacgc gctgattaat attttgaccg agccgaaaaa
     3121 tgctttggtc aagcagtatc aagccttgtt cggtatggaa aatgtcgagt tggaatttga
     3181 agaaggcgca ttgcgttcca tcgcgcgtca ggcaatggag cgcaaaaccg gcgcgcgcgg
     3241 cctgaggtcc atcgtcgaac gctgcctgct cgatacgatg taccgcctgc ctgatttgca
     3301 aggcttgaaa aaagtggtgg tcggtaaggc agtcatcgaa gagggcaggg aaccggaatt
     3361 ggtgttcgag tcctgatggc aaacaaaacc gcttcggaac gttgaaccac gtttccgaag
     3421 cggttttttg tttttgatgc ctaggggcgg tttgcggcgg caaattccgt cagcacgtgt
     3481 tccaaacgcc gcaaaccttc cgccgtatcc tcatcgtcca acagcagcga aggcgcgaaa
     3541 cgcaatacgt ccgcacccgc aaccaaaatc atcacgccgt gtttcaaggc ggcggcggtt
     3601 atttcggatg cgcgtccgcg ataaggcgcg tccaacacgc agccgagcag cagtcccatc
     3661 ccgcgaacct gtgagaacaa gcctgttttc ctgcccaaat ccagcaatgc cgtctgaagt
     3721 ttctgcccct ggctgcggac gttgtgcaag gtttccggcg cgttgatgat gtcgaaggcg
     3781 cggctgccga ccgcgcacgc catcgggttg ccgccgaaag tcgagccatg cgtgcccggt
     3841 tggaaggcgg cggcaatctt ttctgtcgcc agcatcgcgc cgatcggaaa gccgcagccc
     3901 aaggcttttg ccgaactcaa aatatcggga acaatgccgt aatgttcata ggcaaacagc
     3961 ctgcccgtat gccccatccc ggtttgaact tcgtccaaaa tcaacaacgc attgtgccgg
     4021 tcgcacagac ggcgcgcggt ttgcaaatat tccgcagtgg cgggcaggat gccgctttcg
     4081 ccctgtatcg gctcgatgat gaccgcgcag gtctgttcgc cgacggcagc ttccagcgcg
     4141 gcaatatcgt tgaacggaac gtgcgtaatg ccttgcggca ggggtgcata atccttgctg
     4201 tatttcggct ggccgccgac ggacacggta aacagcgtgc gtccgtggaa actgttgata
     4261 caggcgacga tttcgctttt tcctccgccg aaacggtcgc gcgcatattt ccttgccagc
     4321 ttcaacgccg cctcattcgc ttccgcgccc gagttgcaga aaaaaacctt gtcggcaaaa
     4381 ctgtttgcaa ccaatttttg cgccaattcc tgcgccggac gcgtcgtata gatattggaa
     4441 atgtgccaca gcttgtgcat ctgcgcgttt aaagcatcga caagggcagg gtggcagtgt
     4501 cccagcgcat tgacggcgat accgcctgaa aaatcaatat attcacgccc ttccgtatcc
     4561 caaacgcggc tgcctgaagc acgttcggga atcatcgggg caaaggcgaa attgggggtc
     4621 agatagtttt gcataattgc tcctgattgc ggttttgctc gggattatgc accgtttttc
     4681 aaagcagata aagggaaatt gccgacaata tcgggcagcg gtaaaaataa accgaaatat
     4741 tttcaatcgg ataggtgaaa aatcaaaaaa atttcgataa aatgcttgag gcgcggcagc
     4801 gttttgattt agaatctccc caatattttt atcttcagac ggcatttcat catgcaaggc
     4861 aaaaaattta caaccgctta ttgctattgg taccacacag acgtgcggta cttttttgcg
     4921 cttgcttgaa atccatccca tacgcaaaac aggccgctcg aaaacggggc ggcttttttg
     4981 ttaccttcac acaggtcgcc cgattcggca acacacgaca gaatcaggaa aatgaccatg
     5041 aatgcaatgc cccaaaccgc cgcacaaaac gaaaccctgc acacttgcag cattattgtc
     5101 gatgatgcac aggatttttc ccccatgctt ctcgatgcag ggcgggacga cacacttatc
     5161 agcgaatcga tgattccgca aatccgcact gttgccgccc tgatagccgc cgaacggcac
     5221 gacttcagcc gcagcagccc cgccgagttt accgatgccg ccgatttttt tgccgcacgg
     5281 attctcgtat tgggcgtgcg acgcttccat ctggacgtat cgctgcttca gattttgaaa
     5341 accgccaaca gccgcgcccg ccgctttgcc gaaaaacacc gcctgttttt cacacccgca
     5401 caagccgaat tgagcctgaa caaacacaaa aaccgccgcc tgctgaccgt agaaaccgaa
     5461 cacgaagtcg aaaacaaagg caacccagtc gccaacagcc tcgcatttgt ccgcaaactg
     5521 cacaccctgc ccctgtaaac atcacgattt gtcggcagaa tgcttgccaa aattcccgac
     5581 ggcgatacaa taaaaccgat tttccaccca aacaggacac acatgaaaaa aatcatcgcc
     5641 tccgcgctta tcgcaacatt cgcactcgcc gcctgccaag acgacacgca ggcgcggctc
     5701 gaacagcagc agaaacagat tgaagccctg caacagcagc tcgcacagca ggcagacgat
     5761 acggtttacc aactgactcc cgaagcagtc aaagacacca ttcctgccga agcacaggca
     5821 aacggcaaca acgggcaacc cgttaccggt aaagacgggc agcagtatat ttacgaccaa
     5881 tcgacaggaa gctggctgct gcaaagcctg gtcggcgcgg cggcaggcgc gtttatcggc
     5941 aacgcgctgg caaacaaatt cacacgggca ggcaaccaag acagtcccgt cgcccgacgc
     6001 gcgcgtgcag cctaccatca gtccgcacat cccaatgcgc gcaccagcag ggatttgaac
     6061 acgcgcagcc tccgtgcaaa acaacaggcg gcgcaggcgc agcgttaccg cccgacaacg
     6121 cgcccgcccg ccaattaccg ccgccccgct atgcgcggtt tcggcaggag gcggtaaacc
     6181 cggcgtgcca atgccgtctg aagggctttc agacggcatt tttgcatttg ttaggggcat
     6241 tgttatgttg ccgtttgatt ttcagacggc attttgtttc ccagcgtttg atgtcgggat
     6301 ggcaattctg atttacgtgc gttggcaggg cggcattgtc atgccgccgt tgggttttta
     6361 gacggcatca tatcgaaccg tcagaccata gggcgagcat tcttgcccac cgttgcaagg
     6421 gcgggatttg gtgggcagga atgcccaccc tgcatctgaa cctttccgcg tcattctcgc
     6481 gcaggaagga atttaggtat ttgaatttac ggaaattttt aatcgttgcc aaagcggaaa
     6541 agggttttta ttacagccta atcctttggg tatcaaagcc gggcggtgct gaaacattgc
     6601 aaaaatgccg tctgaaactc gaaatcggtt tcagacggca ttggtttatg cggcaggttg
     6661 gggcttagcg gtctttgtaa cggcgttcgc ctctgcctgc aaaagaatcg ccggctttgc
     6721 gtttgccttt gaagccttcg ccggtttttt tgaaaccgtc tttcttaaag ccttctttct
     6781 tgaaaccttc gccgcgcgtt ttgccgccga agccttcttt gcccggttta tgatcgccgc
     6841 gccggccgcc ggatttccta tcgccccagc cgcctttgcc ttttggctta ccgcctgcgg
     6901 atttgcgttt gcgggtcggt tccatgcctt cgatggtcag ttcgggcagt ttgcggttaa
     6961 tgtatttttc gattttgtgg actttgacgt attcgttcac ttcggcaaac gtaatcgcaa
     7021 tacccgtgcg gcctgcgcgg ccggtgcgcc cgatgcggtg gacgtagtct tccgcctgtt
     7081 tcggcaggtc gtagttgata acgtgggtaa tggtcggtac gtcgataccg cgtgcggcaa
     7141 cgtcggtggc aaccaaaatt ttgcagcggc ctttgcgcaa gtccatcagc gtgcggttgc
     7201 gccagccctg cggcatatcg ccgtgcaggc agtttgcggc gaaacctttt tcgtacaatt
     7261 catccgcgat gacttcggtc atggctttgg tggacgtgaa aatcacgcat tgatcgatgt
     7321 tggcgtcgcg caggatgtgg tcgagcaggc ggtttttgtg gcgcatatcg tcgcagtaca
     7381 gcagctgctc ttcgattttg ccttggtcgt ccacgcgttc gacttcgatg acttcagggt
     7441 ctttggtcag tttgcgcgcc agtttgccga ccgcgccgtc ccaagtggcg gagaacaaca
     7501 aagtctgacg gtcgctcggc gttgcttcca cgatggtttc gatgtcgtcg ataaagccca
     7561 tatccaacat acggtcggct tcgtccaaaa tcagcacttc caaacgttca aaatcaactt
     7621 tgccgctttg catcaggtcc atcagacggc ccggcgtggc gacaatcaga tcgaccggtt
     7681 tgctcagggc acgggtttgg tagccgaaag acgcgccgcc gacgatgctg acggtgcgga
     7741 accaacgcat atttttggca tacgccagcg cgtttttctc gacttgagcc gccagttcgc
     7801 gggtcggggt caacaccaaa gcacgcgggc ctttgcccgg tttttcgctg cgtttggtca
     7861 gtttttgcaa agtcggtaac agaaaggcgg cggttttgcc ggagccggtt tgcgccgaag
     7921 ccataatgtc gcggccttcc aaagcaaacg gaatcgcctg cgcctgaatc ggcgtcgggc
     7981 tttcgtaacc ctcgctgctg acggcggaca aaatgttttt atcaaggttc aaatcggcaa
     8041 atttaataga catggctatc cttggaagac aacaacgccc gcgtccgaaa ggttcagacg
     8101 gcctgaagca gggaagggta acgatacggg gaatgtgaag ttacagggtt gtcgcaagcg
     8161 gcttgcttgt ggtttacatc gacggcaacc tgcacagata cgctttgcaa aatcaaagca
     8221 acagaagaaa cgcgcccgta agggcggttt aggttggaag gcgatggctt cgtatgacaa
     8281 gataagcggt ggatggtata gggtttgccc gaaacggtca agcaatcggg cgttaattac
     8341 agtaaaattg ccgttttcca ctttattccg cctgaaacca atgaccgaca tcacaccgtt
     8401 tgccaaccgc ttgggcaaaa acatcaaaca ccttatgaaa tgggcgaaac gcaacggcat
     8461 cgaagcctgg cgcatttacg accgcgacat tccccaattc ccttttgccg ccgatgttta
     8521 cggtgatcga atccaccttc aggaatacga taccggctgg ctgatgcagc ccgaagaata
     8581 cgaagcgtgg cttgccgaag tattggaagc cgtcgttttc gttaccggtt ttgcgcccga
     8641 acaaatccac ctcaaacgcc gcgaacgtca aaaaggtttg cagcaatacg agaaaaccgg
     8701 caaaaccggc gatgattttg tcatcaccga aaacggccgc aagttttggg tcaacctcga
     8761 caaatacctc gataccggcc ttttcctcga ccaccgcaac acgcgcaaaa aagtcggcga
     8821 aaccgcagcg ggcaaacgct tcctcaacct gttttcctac acaggcagct tcaccgtcta
     8881 cgccgccacc ggcggcgcgg catccagtga aaccgtcgat ttgtccaaca cttatctcga
     8941 ttgggcgaag cgcaattttg aattgaacgg catcgatacg gaacggcaca aaatcgtccg
     9001 cgccgacgtg ttccaatatc ttcagacggc atatggggaa ggcaggcggt ttgacctcat
     9061 tgtgatggat ccgcccagtt tttccaacag caagaaaatg tccgacatcc tcgacattca
     9121 gcgcgaccac aaaaagctga ttgacggcgc ggtgaagctg ctcgcttcag acggcatttt
     9181 gtacttttcc aacaacctgc gcagcttcgt cttagacgac ttggtatcgg aacaatacgc
     9241 cgtcaaagac atttccaaac aatccgttcc cgaagatttc cgcaacaaaa aaatccatag
     9301 gtgctgggaa atccgccacg aatcttaagg gtgcgaaaaa gccgccggaa tcttccggcg
     9361 gctttttcga ttgcgcttta tcaggcgata gaggcttcga caaaggcggt cagctgacct
     9421 tttgccaatg cgccgacttt ggtggcgacg acgtcgccgt ttttgaacac catcagggtc
     9481 ggaatgccgc gcacgccgaa acgggacggg gtggcttcgt tgtcgtcgat gttgattttg
     9541 actactttca gacggccttc aaattcggcg gcgatgtcgt ccaaaatcgg ggcaatcatt
     9601 ttgcaggggc cgcaccacgg agcccaaaag tccagcagga cggggaggtc ggatttcaaa
     9661 acatcttgct cgaaagcggc atcgccggtg tgtacgatta attcgctgct cattattttt
     9721 ccttttcggt tgggattggc acgatagcgt gcaggatagg gcttgggcat aaaaatttca
     9781 agtgggcggg cgggataata gtttttctgt accgccccaa tcgcctttgc aaattctaaa
     9841 aaacggttcg gacagtattt tatttctgaa aattactttt attatcaaaa tgtaaggaat
     9901 acgtttgagg ctgtacagaa gcgcgcgggc ggtgtatgct ccgtttgaat tgattcttaa
     9961 agtatgaatg ttcaaaacca aacccgaaac cctgtaaaag gaacactgcg atgactgatt
    10021 tttccgtttg ggagaccgcc ccctttggtg caaccgtcga tcatatcctg caacgctacc
    10081 acaatgtcca ccgcgcacaa tttgaagaat tggtgccgct ggcgcagaaa gtggcccaag
    10141 tccatgccga tacctttccg gcagaaattg ccgggctgct tgccgatatg cagaacgaac
    10201 tgctgatgca tatgatgaag gaggagcgga tgctgtttcc gatgattaat cagggtgtcg
    10261 gacgcggtgc ggcgatgccc atcggcgtga tgatgcacga acatgaagaa cacgaccgcg
    10321 ccatcgcacg gctcaaagag ctgaccgaca atttccaacc gccagaaggg gcttgcggaa
    10381 gctggacgcg gctttacgcg ctggcgaaag aaatggtgga ggatttgaac gaccacattc
    10441 atttggaaaa cgatattttg tttgcccgcg tactcgattc ttaagggata ggagtcggat
    10501 atttccatgc cgtctgaaat acggttttcc gtttcagacg gcatttccac gtttcagccc
    10561 aacaattcgg gcggaatgcg gcacacggga atcgggttga ttttgtgctg catcgcgcgg
    10621 tgcagccgcg tatagatttc cagaacttcg cgctgccgcc cttcaaaatc ttcgggtttg
    10681 cgcgtgccgt acacgcccat tgcccactcc agttcgggat agcttgcgcc catctgttct
    10741 tcgtcggtgc gttccgtatc ccacaggccg tcggtcggcg gggctttttg aatcgcctcg
    10801 tccacgccca atgcttcggc aagccggtaa acctgggttt tggtcaggtc ggcaatcggg
    10861 ctgatgtcca cgccgccgtc gccgtatttg gtaaaaaatc ctacaccgaa atcttcaatt
    10921 ttgttacctg tccccgtaac cagtagtccg tgtatctgcc cgtagtagta cagggtcagc
    10981 atacgcaggc ggcttctggc gttggcgagg gaaagcggct gattgtcaaa tgccgtctga
    11041 tgaacgccga cggtttgttc aaaggtctgg aaggtgtcgg tcagatcgac cgtttgtgcg
    11101 cttacattgg catattgccg ttgcagattg cggatgtgca gccttgcccg ctcaagctgg
    11161 tcggggtgtt ggcgtatcgg catatccaga agcagcgtgg tgcggccggt gcgggcggcg
    11221 agtgcggaga cgacggcgga atcgatgccg ccggaaacgc ccacgacaaa cccttttgca
    11281 tttgcccggg cggcgtattc gtccaaccat cggacaatat gtgtgataac tgcctgcgta
    11341 tccatttttc ctcctgtcga aaatatccgc atcatagctt gtcggcggtg taatttgaat
    11401 agattgaggg cgggttgatg aatatttttc ccattccccg cttaaaaaag tcatacggga
    11461 aaaggcgggc ggcaagatgg cgcgtgttat aatattgttg acaatttagg cggactaata
    11521 ccgccttagg cttcaaaaca tattcatatt ttttattatt tgattgattc tatgtctgat
    11581 ttttttcatt ctgacgtgtt gtccgtttcc gagttgaacg catttgccaa aagtcttttg
    11641 gaaaaccacc ttgccggcct gtggattgcg ggcgaagtgt ccaacctgac ccgtgccgcc
    11701 agcggacatt attatttctc gctcaaagac agccgcgcgc aggtgcgttg cgcgatgttc
    11761 aaaggtgcgg cggcgcgttt ggcgaagcct ttgaaagaag gcgaccatat cgaagtatca
    11821 ggaaaaatca gtatttacga ggcgcggggc gaatttcaaa ttaccgtgaa tgaggtgcgg
    11881 ctcaaaggtt tggggcagct ttacgaagcc tacgagcggt tgaaggcaca gttgcaggcg
    11941 gaaggcgcgt ttgcggcgga acgcaaaaag ccgttgccta cccgtccgca atgtatcggc
    12001 atcgtcacca gcctggcagc agcggcattg cgcgatgtcg ttaccacctt gaaacgccgc
    12061 gcgcccgaaa ttcccgttat cgtttatccg acacctgttc aaggcgcagg cagcgagtta
    12121 caaattgccc aagcgattaa aaccgcctcg caacgcgccg aatgcgacgt gttgattgtc
    12181 tgtcgcggcg gcggcagcat tgaagacttg tgggcgttta acgaagaacc ggtcgtgcgc
    12241 gcaatcgaag cctgcacggt tcccgtcgtc agtggcgtgg gacacgaaac cgattttacg
    12301 cttgccgatt ttgtcgccga cgtgcgcgcg cccacgccga ccggcgccgc agaattggtc
    12361 agccccaacc gccaagaatc gttacaccgc ctcgcccaag cgcaaggcca tctgaaaacc
    12421 attttggaac aacgctattt cgatgccagc caaaaactcg actggctggc gcggcaaatc
    12481 cgtcatccgc gccaaaaact tgacgatcag cgcacctaca ttcacaaact ggtgcaaacc
    12541 ctgtcctact cgatgacgca aaacctccgc gcccataccg cccgttttga acgccaaacc
    12601 caaaccctga aacattgccg tcctgatgtt tccgtttaca aacacaacat cgaccgcttt
    12661 cagacggccc tgtcgcacgc cttccaccaa ctgcttaccc accgccgcca aagcctgacc
    12721 gcccaagcag cattgctcga agccgtttcg ccgcagcaca ttctggagcg cggcttttcc
    12781 gtcgtcaaaa acacacgcgg acaagtcatc cgcaacgccg atgttttgaa gcaggggcag
    12841 aagctgcaca ttatcttcgc cgacggcgaa accgacgtgc gcgtaagcaa agaacaaggg
    12901 cagcaggatt tgttcgattg catttaaacc caatcggaaa aaccgctccc cctccgccgt
    12961 cattcccgcg aaagcgggaa tccagtccct tgaccttaag ataatcttta attcgtcatt
    13021 cctgaaaaag cgggaatccg gaagcgaaaa gtggtgggaa tgtattagaa atgactgaaa
    13081 ctcaaaaaac cggattcccg cctacgcggg aatgacgagt cagaagttac tcgaaactta
    13141 aaaaaaacga aatcgaacgg actggattcc cgcctgcgcg ggaatgacga gtcagaagtt
    13201 actcgaaact taaaaaaccg aaatcgaacg aactggatgg attcccgcct acgcgggaat
    13261 gacgagtcag aagtgactcg aaacttaaaa aaccgaaatc gaacgaactg gatggattcc
    13321 cgcctacgcg ggaatgacga gtcagaagtt actcgaaact taaaaaaccg aaatcgaacg
    13381 aactggatgg attcccgcct gcgcgggaat gacgattgat acgtttcgat aatttattga
    13441 agatggctga aatccaaagt tacagaaatt tataaacaaa aaacagcaac ccgccgccgt
    13501 cattcccgcg aaagcgggaa tccagtccct tgaccttaag ataatcttta attcgtcatt
    13561 cccgcaaaag cgggaatccg gaagcgaaaa gccgcaggaa tttgtcggaa aaaacagaaa
    13621 cctttccgta cacccgaaat gtttcagacg gggcatcttg gggcggataa aaaaccgatc
    13681 cgatattttt ttatagtttt caataagttt ccattgagca aatgaaattt tatttttcaa
    13741 ctgacagaaa gaggatgtta tgaagttttt agaccgtgag gcaagcattg ccaagccggg
    13801 tttcaaccgt tggctcgtac cgcccgcagc attggcggtg caccttgcca tcgggcagat
    13861 ttacgcctat tccgtattca acgcgccgct gaccaaactt atcggcataa ccgaatcggc
    13921 ggccggagat tggaagctga cgaccgtagg ttggattttc agtatcgcac tcgcgatgct
    13981 gggcgcgtcg gccgccctgt tcggtacgtg gatggaacgg gtagggccgc gcaaagccat
    14041 atttgccgcc gcctgctgct tcagcctggg cttcttcgta tccgcattcg gcgtgcgtac
    14101 gcacaacctc ttcctgctct atttgggcaa cggcgtcatc ggcggcgtag gcttgggttt
    14161 gggctacatc gggccggttt ccacactgat gaaatggttt cccgacaagc cgggtatggc
    14221 gaccggtttg gcgattatgg gtttcggcgg cggcgcaatg ctggcctcgc cgctttccgt
    14281 atcactgatg aacgcctttt caaacgctgc ttcggttggg gttgccgaaa ccttcgccgt
    14341 attgggtctg ctttacctcg cactgatgat gtttggcgcg tttaccatcc gcgtacctgc
    14401 cgacggctgg aaacctgaag gctataccgc gccgaaaacg caaaacaagc tggtcagcag
    14461 caatcatgtc aacgtgtccc aagccattaa aacgccgcag ttttggctgt tgttctgggt
    14521 attgtgcctg aacgtaactg ccggcatcgg cgtattgggt caggcatccg tgatgattca
    14581 ggaactcttt tccgaaacct ccgtaggtag gcaggcggca gtcggcgcag gtgcggcggc
    14641 aggcttcgtc agcctcctga gcctgtttaa tatgggtggg cgctttttgt ggtcgagcgt
    14701 ttccgacaaa atcggacgca aaaacaccta caccatcttc ttcgtactcg gttcgctgct
    14761 gtatttcgcc gttccctcca tcggcgaggg cggcagcaaa gccctgttca tcatcggctt
    14821 ttgcgtgatt atttccatgt acggcggcgg atttgccgcc attcccgcct acctgaaaga
    14881 cctgttcggc acctatcagg tcggcgcgat tcacggacgc atcctgctgg cctggtcaac
    14941 cgccgccgtc atcggcccgg tactggtcaa ttacatacgc caaagccaaa tcgacagcgg
    15001 cataccggcg gcacaggcat acagcgtaac catgtacatc atggcgagcc tgctgattat
    15061 cggtttgttg tgcaacctcg ccgttaaatc cgtacacgaa aaataccacg aaaaagacat
    15121 caagacagcc gcacgcagcg gtaaccccga cgacgaaacc gccatatccg acgcttacct
    15181 tgtgggcgaa aaagtttccg gcggcggcat atcggtctgg tggcgttggg cattggccgt
    15241 cataccgctc gcctacggtg tggtgatggt ctttgtcaaa gcactcgacc ttttctcctg
    15301 aaaacgcccg aacaaaatgc cgtctgaaac agcaggtgtt tcagacggca tttttgtgtt
    15361 ttggcgggtt taggcttttt tgcggcggcg ttcgcccggc agcagcattt ccgcccattt
    15421 gaggatgttg gcgacttcgg cggggttgag ttcgtagaac tgtccgcgtt tgaggcggtt
    15481 gggcagtccg atggggccga agccgacgcg cacgaggcgg ctgacggtga gtccttggct
    15541 ttcaaaaatg cggcgcactt cgcggttgcg gccttcttta atcacgacgt tgtaccattt
    15601 gttcgcgcct tcgccgcctt gttcgcggat gcgttcgact tttgccaagc cgtcttcgag
    15661 catcacgcct tcttcggtga ggacgcgcat ttgttcgccg gtcagcccgc ccaagacgcg
    15721 cacggcgtat tcgcgttcga cttcgaagct ggggtgggcg aaacgttgga cgagttcgcc
    15781 ggaggtggtg agaatcagaa gtccgctggt gttgatgtcc aagcgtccga tggcgaccca
    15841 gcggctgctg gcggcctgcg gcagacggtc gaatatgctg acgcggcctt gcggatcgtc
    15901 gcgggaaacg atttcgcctt cttgtttgta atacaggatg atgcgcggca ggcggtccgc
    15961 ccatttgagt ttgatgatac tgcctttgac ggtaacgtgg tcgtcggggg taactttgtc
    16021 gcccagttgc gcggttttgc cgttgaccgt tacccagccg ttgttgatcc attcttccat
    16081 ttcgcggcgc gagccgacgc cggacgcggc aagcactttt tgcaggcgtt cgggttccat
    16141 gcgcgacagg tcgctgcggc gttctttcaa atcgcgcgcg tgttccataa tcttttggtt
    16201 gggattgcgg acgacgagtt ttttggcttt ggcggcgcgt tgtttggggg tgttttgcag
    16261 cttgatgccg tctgaagctt tttgtccgta aggttgggat gcggttttgc ccgtttcgtc
    16321 tttgggacgg gctttgcttt tgaaaggttt ggcggttttc ttggcagacg gggctgcgcc
    16381 gtcgcgccat tggcgtttgc tggtgggttg cttggacatc atttttccta actcgcctga
    16441 taaggcggtt cttctgcggc gtgtgcggca ggacgggttg ataaaatgcc gtcctcagtc
    16501 ggacggttcg atattgtacc tgatgcggcg gttttgcgga tgaaatcgtc gctgcggctt
    16561 gtggcgaggc aaagtttgtg caacggttga aatcggtttg cgtgcggggg tagaatcttt
    16621 caaatgacaa gaggagcggt aaatggattt gcacaatatt cgggaagatt acagcaaacg
    16681 ggaattgtcg gaaggcgatt gcgccgacaa tccgatcgag cagttcgagc ggtggttgga
    16741 cgaggcggta cgcgcacagg tcaacgagcc gacggcggtc aatgtggcgg cggttgacgg
    16801 acgcggcagg cctaacagcc gtatggtgct gctgaaggaa gttaattctg aaggttttgt
    16861 tttctttacg aattatcaca gccgcaaggg gcgttcgctg gatgctcatc catttgcggc
    16921 gatgacgttt ttttggccgg agctggagcg tcaggtgcgc gtggaagggc gtgttgaaag
    16981 gctggcggag aaattgtcgg acgaatattt tgagagccgc ccgtatcaga gccgtttggg
    17041 tgcgtgggca agcgcgcaga gcgaagtgat tccgaacaag gcggtattgg tggcaaaggc
    17101 ggcggcggtc ggactcaaac atcctttgca cgtgccgcgt ccgccgcatt ggggcggcta
    17161 tattgtgatt cccgatttgc ttgagttctg gcagggcagg ccgagcaggt tgcacgaccg
    17221 catccaatac cgtttgctgg atggtggctg gatacgcgag cggctgtcgc cttgaacagg
    17281 aaaaaacata tgccgtctga aaaccggaat cgggcttcag acggcatttt ttatggggaa
    17341 aatacggacg ggaagttttt tgtgttccga acggaaagcc tgaatctttt ttataccggg
    17401 gaattatgat gcggacggcg tgaatgcgtc ggagaaaaac aagttttccg gcagcttatg
    17461 ctgttgcaca aacagattct ttgtttgttc ggtcatggcc ggagaaccgc aggcaaatac
    17521 ttcgtattcc gacaggtcgg ggtggtcttg tgccgcgatg tcttgtacgt ggccttttct
    17581 tccctgccag ccctctccgg ggcgggacaa tacgggggtg aagcaggcgt ttttcagacg
    17641 gcatgccaac ccttgtgctt ctttgagggc atacaaatca tcttgatgac gcgcgcccca
    17701 gtagaaatgg acggcgcggc tgctgtcttg gcggataagg tcgagcagga tgctgcggat
    17761 gggggcgtag cctgtgccgg ttgccagcag gatgacgggc ttgccgctgt cttcctgcaa
    17821 ggtaaacgaa ccgagcggac ctttaacgcg gacgatgcct ttttctttga ctttgggttc
    17881 gctgccgaaa atcatttccg agcagacacc gttttcgcgc ctgcggatgt gtagttccaa
    17941 aatgccttct tggtcgggcg gattggcgat ggagtagctg cggctgacgt tgcccggcag
    18001 cagtaaatca atgtattgcc cggcgtagaa ggcaaacggc ggggctttgg gcagggcaag
    18061 tttcaagagg gcgacatcgt gtttgaaaac catactttcg atgcgtgcgg gcagggtgcg
    18121 gacggggagg gcgttggggt tgtagccggg gatgttgagg ctgatgtcgc tttgcgcggt
    18181 ggtgcggcac atcaaaatct tgccttgcgc tttttctgct tcggataaag cctgttcgga
    18241 gtgtccgccc atttgaatat cgccgctgac cagttcggct ttgcattgcc cgcagacacc
    18301 gtttttgcag gaatggggca gattaaggtt ttgtcgggcg gcggcggaca aaacggtttc
    18361 accgtcgttg gcggcaaagg tggtttggtc gggcagggta acggtgtggt tcatggaata
    18421 ttgtttttta tgctgtttta atgtggtcag actgtgaaaa tgccgtctga acgtcggatt
    18481 tgggtttcag acggcatctt gcttatcagg caagtttggc tttaatcagc tcttgaacct
    18541 gcgcggggtt ggctttgcct ttgctggctt tcatcacttg tcccacaatc gcattcaggg
    18601 ctttttcgtt gccggattta aactgttcca cggctttggc gttgtttgcc agcacttcgt
    18661 ccaccatcgc ttcaaccgcg ccggtatcgg tcatctgttg caaaccgtgt ttttcgatga
    18721 tttcggcaat agtggcttcg ggttctgccc acatggcttc aaaggctttt ttcgctaatt
    18781 tgctgcttaa tgtgccgtcg gcgattttgc ctaccagcgc ggcgaggcgc ggagcggtaa
    18841 tcgggctgtc ggcaagttcc atgccttctt tgttcagtgc ggcggcgagt tcgccgttca
    18901 tccagttggc agtcggcttg ccttgtccgc tctctttggc ggcttcttca aaataggcgg
    18961 cctgtgcgcg gcttgcggtg agcaggcgcg cgtcgtattc ggacacgccg tagtccgcca
    19021 cgaaacgcgc tgccatttct ttcggcagct cgggcatttc tgctttggct ttttgcattt
    19081 gagcgtctga aatgatgacg ggcagcaaat cggggtcggg gaagtagcgg tagtcttgcg
    19141 cgtcttcttt caggcgcatt acgcgggttt cgcctttttc gggatcaaac agcatcgttg
    19201 cctgctgtac tttgccgccg tcttccaaaa tctcgatttg cgcttctgct tcgtaattaa
    19261 tcgcctgctc caagaaacgg aaggaattga ggtttttaat ctcgcggcgc gtgccgaatt
    19321 ccgcttgacc tttcgggcga acggatacgt tggcatcgac gcggaacgaa ccttccgcca
    19381 tattgccgtc gcaaatgtcc agccaggtta ccaagctgtg caaggctttg gcgtaggcaa
    19441 cggcttcggc ggcggagcgc aattccggtt cggatacgac ttccaataaa ggcgtgccgg
    19501 cgcgattcag gtcgataccg gtcgcgccgt tcaagccttc atgcacggac ttgcccgcgt
    19561 cttcttccat atgggcgcgg gttacgttga tggttttcac atcgtcgccg actacgattt
    19621 ccaatttgcc gtgttcgaca atcggtaagt ccaactggct gatttgatag ccttttggta
    19681 agtcgggata gaagtagttt ttacggtcga acacgttttt ccggttgatt ttcgcatcca
    19741 aagccaaacc caatttgatg gctttttcaa cgacttctcg gttcattacc ggcagtacgc
    19801 ccggcaaagc acattccacc acgctggcgt gcgcgttggg ttctgcgccg aatgcggtcg
    19861 atgcgccgct gaagattttg gatttggtgt tcaattggac gtggatttcc aagccgatta
    19921 cggtttccca ggtcataaga gcttctttct ctaaaatatc aattcgatag tcttcgcgtc
    19981 aggctgtccg gcggagcggt ttttccgttt ggatgccgtc tgaaatgccg cgtgcttctg
    20041 tttcagaccg tccttcatct ctgcaaccat gttgtccagc atttcataac gcttgcggta
    20101 catcgagcgt tttttgcttt cgatgtcggt aaggtctttg cgggcggggg tttgggggag
    20161 atgccagtag ccgtcccgtt ccaaacttgc gccgtattcc tgccagaatg cgtcgtaatt
    20221 cattttgaca cgctttttaa gtttccagcg cagtttgacc tgatgttttt gtgcaatgcc
    20281 cattgcgccg tccaagccga gtacggcggc gaaatattgc agggcggtta ccatcagttg
    20341 ttggggacgc aagccgtgaa gttgtttggt tatgcggcgg acggtgtctt tggcttcttc
    20401 acccgccggc ccttgtacgg aggctgtcag caggtgtgtg ccgacaaagg cgaaggtcgc
    20461 catatacagc cggttgcctg cttcgtcgcg caaagataaa gaccacgcgc cttcttcgac
    20521 gcagttgtcg ttgcggttga gccacaagct gatgccgtct gaaaggtgcg ccaagaccag
    20581 atggaagcgg tagtcttcca tttggtgcag gatgtctgtg ccgaacagtt tttccgccat
    20641 gagaaaatct gccgtcatcg catctaaacg gcgtttgcag ttaaaacgcc tgtcaacaaa
    20701 ttcgcgcagc agcggatagg cattttgcgg gcagtggagg aacagcccct gacggatagg
    20761 ggatgcgttg acaaactgct cgaaggcgta aatttctttt tttccgaaaa attttcgcaa
    20821 tgtgtatttt aaatgttgca gccgatataa aggcgcatag ctgtaaacgg tgcgaaaatc
    20881 ggggaaggta aaggtgcggt tctgcttcat tcgggtcgtt tgccgtgcca atcgctgttg
    20941 agttggattt gatgcgccgc accgaggatt ttggcttcgg cgaagtagtt gccgacaagc
    21001 tgaacgccga cgggcagtcc gccgccgctg aagcctgcgg gcagggtcaa tgcgggcagt
    21061 cctgcgaggt tgacggcgat ggtatagata tcgctcaagt aggtttcaac cggcgaagca
    21121 tccgctccga ttttgggggc ggcagtgggt gcggtcggcg cgaggatgag gtcgcaccgt
    21181 gcaaatgccg tctgaaaatc atcggcaacg aggcggcgca gtttttgggc tttgagatag
    21241 taggcatcgt agtagccgtg cgacagtaca taagtgccga tcatgatgcg gcgtttgact
    21301 tcgctgccga aaccttcggc gcgggttttg ccgtacattt cttccaaatc gccgaattgg
    21361 gcggcacggt gtccgtagcg cacgccgtcg taacgtgaaa ggttggtgcc ggcttctgcg
    21421 gaggcgagga cgtagtaggc ggggatggac agcttggttt gcggcaggga aacttcaatc
    21481 aattccgcgc cttgggcttt cagcaaatca atggtgtttt gcaatgccgt cagaacatcg
    21541 gcgctgttgc cttcgccgaa atattctttg ggcaggccga ttttcaaacc tttgagcggt
    21601 tggttcaaat cgcgggtgta gtcttctttt tcgcgctcga ggctggtgga gtctttcggg
    21661 tcgaaacctg ccatcgcgtt taacagaatc gcgcagtctt cggcagtttg cgccatcggg
    21721 ccggcttgat cgaagctgga ggcgtaggcg accataccga agcgggaaac cgtgccgtat
    21781 gtgggtttga tgccggtaat gccgcagtgc gatgcaggct ggcggataga gccgccggta
    21841 tccgaaccga gcgcggcagg ggcaaggcga gcggcaacga cggcggcgga accgcctgac
    21901 gaaccgccgg ggacgtgttc cggattccac ggatttttgg ccgcgccgta gaatgagttt
    21961 tcattggtcg aacccatagc gaactcatcc atattggtgc ggccgagcgt taccatacct
    22021 tcgtcgagca ggttttggac gacggtggcg gtgtaggggg agatgaagtt gtcgagcatt
    22081 ttggaagcgc acgcgctgcg ccagccggtt tggcagaaaa tatccttgta ggcgacgggt
    22141 acgccggtaa gcgcggaggc gttgccctgc gcgatacgtt cgtcggcggc acgggcttct
    22201 gcaagggttt tatcttggtc gatggtgata tagccgttga gggcgggatt tttttcggcg
    22261 atggcggcaa ggtatgcgct tgccagttcg acggcggaaa tctgtttgga ctgcaacagg
    22321 acgcttgcct gtttcaatgt gtattgggtc ataaatcttc catccttaaa gggcttcggg
    22381 cggtattgct gatgccgtct gaagcatatt cggattattc ttcgataact tgcggtacga
    22441 tgtacagacg gttgcgtact tccggagcac ccgcctgata ttcggcggcg cggtcggttt
    22501 cggttacttc gtcttcgcgc aggcgcaggg cggcctcgtg cgggtgcgcc atcggttcga
    22561 tgccgtctgt gttaatggtt tgcatctgtt cgaccatagt gaaaatgtcg tttaattctt
    22621 gaagcgattt ttctttttct tccgcagtca ggtgcagtcg ggagagtcgg gcgattttgt
    22681 ctacgtcggc aagtgtcagt gccatgaaaa gcatccttgt ctgatggtaa agataccgtc
    22741 gttcgggtat gatggggcgt attgcttgaa tttttaatta taacggaaaa atccgttgcg
    22801 gcggcaattt gcagcatcgg atgcctgccg cacgggtttg aaggaggggg aaataatggg
    22861 attgggcatg gaaatcggca agctgattgt ggcttttttg gtgctgatta atccgtttag
    22921 cgcgttgtcg ctttaccttg acctgaccaa cgggcacagc acgaaggagc gcaggaaggt
    22981 cgcgcggacg gccgccgttg ccgtgtttgc cgtgattgcg gtatttgcgc tgatcggcgg
    23041 tacgctgctg aaggttttgg gcatcagcgt cggttcgttt caggtcggcg gcgggatttt
    23101 ggtgctgctg atcgccattt cgatgatgaa cggcaacgac aatcccgcca agcagaatct
    23161 cggcgcgcag ccggaaacgg ggcaggcgcg ccccgcccgc aatgccggag cgattgccgt
    23221 cgtgcccatc gccataccga tcaccatcgg cccgggcggt atttcgaccg tgattattta
    23281 cgcttcggca gccaaaacgt acagcgacat cgcgctgatt atcgtggccg gtttggtggt
    23341 cagtgcgatt tgttatgcca ttttaattgt tgcagggaag gtcagccgcc tgctgggcgc
    23401 gacggggctg acgattttaa accgtatcat gggtatgatg ctggcggcgg tatcggtgga
    23461 gattattgtg tcgggactga aaacgatatt cccgcaactg gcaggttgat tcgatgtttc
    23521 acagtgtcaa tgtatttacg ggcgaaacgc tttaccgccg tccagctcaa gattatgcgg
    23581 agtttgaacg gcggctggcg gatttgaaaa tgcgcggcgg ggcgttcgcg caactgggcg
    23641 taaccgaacg tgccgcgcgt ctgcaaaaat ttgccgaccg tttggaggcg gagaaagagc
    23701 gttttgcgga aatggtgtgc gaagaagtcg gacgctgcct gcacgaatgc cgtgcggaaa
    23761 tcggcaagtc tatcgaactg atacgctatt acgcccgcct cgctcccgaa ctgctcgccc
    23821 acaaaaccat cgcgacgcag gcgagtttga gtcaggtaag gtttgagcct ttgggcgtgg
    23881 tgtttgccgt catgccttgg aactatcccg tctggcaggt attgcgtttc gccgttcccg
    23941 ctatgtgcgc gggcaacgcg tgcgccgtca aacccgcgcc cagcgtggca cgcgtcagtc
    24001 aggcgttgtt tgatttggtt tcagacggca ttcctttggc aggcgcgtgg ttggatgagg
    24061 cgggcacgct caaggcggtt gaagataccg atgcgatggc gtttaccggc tcgacgcata
    24121 cggggcgcat ccttgcggca catgcgggcg caaacctcaa gaaaaccgtc ttggaactcg
    24181 gcggcagcaa cgcctttatc gtgatgcccg atgccgattt ggaacgcgcc gccgcagaag
    24241 cctgttattc ccgtttccgc gatgcggggc aatcgtgcaa cgccgccaaa cgcatcatcg
    24301 taaccgaagc cgcagccgat cgttttatca cgctgtttct tgccgaatgc gccaaattga
    24361 aaatgggcga ccccaaacat cccgatacta cgcttgcacc gctgcaccgc gaagatttgc
    24421 gggacagggt tcacgggcag gttgaagatg ccgtttcaaa cggcgcggta tgcctgaccg
    24481 gcggaaaaat cccacaaggg cgaggctggt tttatcccgc gacggtttta gacagggtaa
    24541 atcctgcctg ccgcgtctgg agagaagaag tattcgggcc ggccgccctg attttgcgcg
    24601 ccgaaaacga agaacatgcc atcgcccttg ccaatgattc cccgttcggg ctaggtgcct
    24661 gcatttatac cgccgatacc gaacgcgctt ggcggtttgc cgaaaaaata caggcgggat
    24721 cggtgtttat caaccgccat accagcagcg atttgcgcct gcctttcggc ggagtcaaag
    24781 attccggtta cgggcgcgaa ttgtccgaat tcgggctgta cgagttcgtc aacgtcaaaa
    24841 cctactggca gaaataaaca aaccccgtgc cgtttcagac ggcatggggt ttgcgctaga
    24901 gccgattaac ggcagtattt gtttacggcg ttattgtatt tccgaatcaa atcatccttg
    24961 ttttttgcat ttgaatttcc caccgctttc aggttcattt ttgaaatccg gcagttttct
    25021 tctttgttct gccgttcggt ttcggcaatt cttttctttt cttcctcaag ctgccggttt
    25081 ttctcggcag tgtcgggatt gttttcctgt gccgcgccgt ctgtgcgctt ccctgcgtcg
    25141 gcttgggcgg gtttgaccgc cggtttggtt tggcgcgtcc gcaggtttaa gatttggctt
    25201 tggtcgggat gaagctgttt cggtacatcc gaatagctgt tgccgccgcc gtccttccaa
    25261 gtaaagacgg cggctccggc aggcgggacg ggcaggaaag aggcgagggt aaatgtgatg
    25321 acggataaag caaagttcat tagggtttcc ttaagggttg gctgtcgggg cagcatcagg
    25381 attggcttgg gcggtgtctt ttccgggttc gggtcaaagt ggggaagaaa ctccgcctta
    25441 atatggtgct ttatatattg gggtgaaaca gattccca
//
LOCUS       DAWFHY010000022        24420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.022, whole genome shotgun sequence.
ACCESSION   DAWFHY010000022 DAWFHY010000000
VERSION     DAWFHY010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 24420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.022"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(54..3153)
                     /locus_tag="WGQ68_000807"
                     /pseudo
     CDS             complement(54..3153)
                     /locus_tag="WGQ68_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(3190..3293)
                     /locus_tag="WGQ68_000808"
                     /pseudo
     CDS             complement(3190..3293)
                     /locus_tag="WGQ68_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(3338..4555)
                     /locus_tag="WGQ68_000809"
     CDS             complement(3338..4555)
                     /locus_tag="WGQ68_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEZ0714647.1"
                     /translation="MDMQSKAKKLIEMIQTAPVEWKPLGGENGIAIIKTGQAVSKQKI
                     SNNIGSYPVINSGKEPLGYIDEWNTENDPIGITTRGAGVGSITWQEGRYFRGNLNYAV
                     TIKNRTELDVRFLYHILLEFEQEIHALCTFTGIPALNASNLKKLLIPIPPLETQQKIV
                     KILDKFTKLEAELALRKRQYRYYRDFLLDFDNQIGGIADGYKGRLKDVVWKTLGEVFD
                     LKNGYTPSKSNKEYWENGSIPWFRMEDIRENGRILDNSLKHISKSAVKGGKLFPAKSI
                     MMSTTATIGEHALIKVNYISNQQLTNFTIKDEFKDALDINFAFYYFFIIAEQSKKLIN
                     TSSLPIISMKELKKLKIPIPPLPEQEKIAAILDKFDTLTHSISEGLPHEIALRRKQYE
                     YYREQLLAFPKAT"
     gene            complement(4721..5731)
                     /locus_tag="WGQ68_000810"
     CDS             complement(4721..5731)
                     /locus_tag="WGQ68_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
                     /protein_id="HEZ0714648.1"
                     /translation="MSIILYTANDGTAQFALQEFGGQLWLTQADMAELYQTTKQNISK
                     HIKTILAEQELEEKATVNFQLTVQNENGRKVNRKIAHYSLPMIIAVGYRVRSARGIQF
                     RQWATERLDEYLTKGFAIDDERLKGTGGGDYWKELLNRIRDIRSSEKALYRQVLDLYA
                     TSQDYNPKSSESQTFFAAVQNKLHYAASRQTVAELIYSRADSSKDFMGLTTFQGAIPT
                     LNEAKIAKNYLTEDELFRLNRLVSAFFDLAEIKAQEQSPMYMRDWIAELDKFSGLYGQ
                     GTLQGAGSISRKQAEQKAEREYRAYEARILSPVEQAYLESVKALEKTAVQQIKQKKDR
                     TK"
     gene            complement(5728..7272)
                     /locus_tag="WGQ68_000811"
                     /pseudo
     CDS             complement(5728..7272)
                     /locus_tag="WGQ68_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742844.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
     gene            complement(7345..8349)
                     /gene="rfaD"
                     /locus_tag="WGQ68_000812"
     CDS             complement(7345..8349)
                     /gene="rfaD"
                     /locus_tag="WGQ68_000812"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HEZ0714649.1"
                     /translation="MTIIVTGAAGFIGSNIVKALNQRGITDIVAVDNLSKGEKFKNLA
                     ECEIAHYLDKHEFIRQVREHILPYQNIEAVFHQGACSDTMNHDGLYMMDNNYQYTLDL
                     LDWCQDERIPFLYASSAAVYGKGEIFREERELEKPLNVYGYSKFLFDQVLRRRMKEGL
                     TAQVVGFRYFNVYGQHEQHKGRMASVAFHHFHQYREHGYVNLFGSNDGYGNGEQTRDF
                     VSVEDVAKVNLYFFDHPELSGIYNLGTGRSQQFNELAAATVNACRAAEGKPEMSLKEL
                     VEEELIRYIPFPDALKGKYQSFTQADITKLREAGYTEEFFDVKSGVERYVKWMLENLA
                     "
     gene            complement(8390..9508)
                     /locus_tag="WGQ68_000813"
     CDS             complement(8390..9508)
                     /locus_tag="WGQ68_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0714650.1"
                     /translation="MPDFQRITIENIEYFIVDSIQNLRAEDSFIHRNNKLSVFGGNGE
                     ARKYVGSYIDDSGRKLSTFFEYENWGITETKNGRRVEYPLIQKNCFFNKKNLQRYLVD
                     AKVEYHKQEQKYHHDISRFYDDYVLSINNLPTENIFFSIHDVSDHLENSKGYRRGYIR
                     SEDDIWSIWRKIVLPKISYLSILKLLPVKDIEDSEPLFYFRIFLDYQFRSIVHPQLLS
                     GEKLEIPASEFNQFVEQEIIKQKSRKGQQKYRKDVINHMPQCPFTLITDEILLRASHI
                     KPYMVCITEKNEKEALDYLNGLALTPTYDWLFNQGYITFLDDGRLICGTRLSRYTWEK
                     LNINPNAKNKMRIYPEGREKYLEYHRKFVFQDNIDDFL"
     gene            complement(9510..10745)
                     /gene="dcm"
                     /locus_tag="WGQ68_000814"
     CDS             complement(9510..10745)
                     /gene="dcm"
                     /locus_tag="WGQ68_000814"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HEZ0714651.1"
                     /translation="MTISTMAQTHDTRLQKTLLFPSQQGWKSKTFLKPDSHIRLATVF
                     SGIGAVEQAFHRLNLNHTIVFAGDIDPYVKKSYLGNYKLNEDFWHNDITQFDASKFRN
                     QVDILVGGSPCQAFSMVGKRAGLEDTRGTLFYEFARVVDEVQPKIFIYENVKGLLNHD
                     NGKTWKVVKSVFYSLGYDLYFQIMNSKDYGIPQHRERIFVVGFHTPPINGFQFPEKIE
                     LEHTMQDFLEDYTDSKYFLREKGVKFVTSSKNRQKRYTQINGEIALCQKANQQFNWHG
                     DFIFQAARESEFDDFIFDVNNVEEKYYLSEKIKNYVLAGGTKNFKTSTETDLPVARPL
                     LQTMHKMHRAGVDNYVTHNRGRIRKLTPRECLRLMGFRDDFKILVSDTQMYRQAGNSI
                     VVDVLIAILKQMDITLYGE"
     gene            complement(10782..11753)
                     /gene="hldA"
                     /locus_tag="WGQ68_000815"
     CDS             complement(10782..11753)
                     /gene="hldA"
                     /locus_tag="WGQ68_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose synthase"
                     /protein_id="HEZ0714652.1"
                     /translation="MPAKFQQETLKSRFAQAKVLVVGDVMLDRYWFGDVSRISPEAPV
                     PVAKIGRIDQRAGGAANVARNIASLGGRAGLLSVTGDDEAADALDALMVQDGVASYLM
                     RDKQIATTVKLRVVARNQQLIRLDFEEHPNREVLEQIKQKYLEVLSEYDAIIFSDYGK
                     GGLSHISDMIDCAKHEGKIVLIDPKGDDYEKYAGATLITPNRAELKEVVGSWKNEGEL
                     TEKAQNLRRHLDLTAVLLTRSEEGMTLFSEGEPIYQPTRAQEVYDVSGAGDTVIAGMG
                     LGLAAGCTMPEAMYLANTAAGVVVAKLGTAVCSFAELTKALSGQSTM"
     gene            complement(11809..12549)
                     /gene="pyrF"
                     /locus_tag="WGQ68_000816"
     CDS             complement(11809..12549)
                     /gene="pyrF"
                     /locus_tag="WGQ68_000816"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEZ0714653.1"
                     /translation="MNPLISDFQTPQQRTPVIVALDFANEKDTLGFVRNLDPTLCQIK
                     IGKELFTATGRSLAESLINQGFKLFLDLKYHDIPHTVAQACKVAADMGVWMVDMHASG
                     GRRMMEAAAEAVAGYGTKPLLIGVTVLTSMEQSDLAEIGLNIAPEEQVIRLAKLAQSS
                     GLDGVVCSAQEAAPLRRELGQDFVLVTPGIRLDVAGNNDDQRRIMTPAEALAAGSTYL
                     VMGRPVTQAANPLAVLREVNRVANLEAN"
     gene            complement(13075..13722)
                     /gene="adk"
                     /locus_tag="WGQ68_000817"
     CDS             complement(13075..13722)
                     /gene="adk"
                     /locus_tag="WGQ68_000817"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEZ0714654.1"
                     /translation="MKALLLGAPGAGKGTQAQFITAAFGIPQISTGDMLRAAIKAGTP
                     LGLEAKKIIDEGGLVRDDIIIGMVKERIAQDDCKNGFLFDGFPRTLAQAEAMVEAGVD
                     LDAVVEIDVPDSVIVDRMSGRRVHLASGRTYHVTYNPPKVEGKDDVTGEDLIQRDDDK
                     EETVKKRLAVYHEQTEVLVDFYSKLEGEHAPKYIKVDGTQPVEAVKAEVLGALGK"
     gene            13937..14776
                     /gene="htpX"
                     /locus_tag="WGQ68_000818"
     CDS             13937..14776
                     /gene="htpX"
                     /locus_tag="WGQ68_000818"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HEZ0714655.1"
                     /translation="MKRIFLFLATNIAVLVVINIVLAVLGINSRGGAGSLLAYSAVVG
                     FTGSIISLLMSKFIAKQSVGAEVIDTPRTEEEAWLLNTVEAQARQWNLKTPEVAIYHS
                     PEPNAFATGASRNSSLIAVSTGLLDHMTRDEVEAVLAHEMAHVGNGDMVTLTLIQGVV
                     NTFVVFLSRIIANLIARNNDGSQSQGTYFLVSMVFQILFGFLASLIVMWFSRQREYRA
                     DAGAAKLVGAPKMISALQRLKGNPVDLPEEMNAMGIAGDTRDSLLSTHPSLDNRIARL
                     KSL"
     gene            complement(15077..15637)
                     /locus_tag="WGQ68_000819"
     CDS             complement(15077..15637)
                     /locus_tag="WGQ68_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714656.1"
                     /translation="MTANFAQTLVEIQDSLYRVVSTVQYGDDNLKRLTADKRKQYELN
                     FKISEGSTRVESDFKETLVRFGRDMLQDMPPKIRSATLVALTTLLVGGALGYGYLEYL
                     KQVASEGYQTERLYNAVDRLAESQERITSAILKGARGADFVQIGRRSYSREDISEANR
                     RAERVPYGAELVSDGNFTAVLSDIGD"
     gene            complement(15751..16143)
                     /locus_tag="WGQ68_000820"
     CDS             complement(15751..16143)
                     /locus_tag="WGQ68_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0714657.1"
                     /translation="MGKRMTFDTAKSRFQEKFPHLELLEFSGIYKPSSVRCPTHGVVQ
                     LLYYDTAIKSKYGCPECGKLKMKENTPPQNQKPVSILDTATGETLTFPSVQAAAKALN
                     TPYGSIRTKLDGRSNPDNLVCNRYKVLL"
     gene            complement(16351..16743)
                     /locus_tag="WGQ68_000821"
     CDS             complement(16351..16743)
                     /locus_tag="WGQ68_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0714658.1"
                     /translation="MAKSFNQAASELTDIFPNISLTGFDGVNYPVTVNCPMHGNVRYS
                     TFNALIKSKYGCPECAKMSKTQTPPNVGKPLLILDTTTNETLTFPSVTAAGAALGVHF
                     QQINHRLKGRTSPDNLISNRYKVLGYDR"
     gene            complement(16860..17270)
                     /locus_tag="WGQ68_000822"
     CDS             complement(16860..17270)
                     /locus_tag="WGQ68_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0714659.1"
                     /translation="MALTFTQAVSKLTSKFPHLNLVEFNGVRYPTVIVCPIHGRVTCS
                     TFKSMLDSKSGCPKCASYGVNSHKIPEDTIDKLSKNTVLEDTVTGETLTFPSRASAAR
                     SLGINPAAITDRIKGRVHTETLLAGRYKVHICTK"
     gene            complement(17284..17721)
                     /locus_tag="WGQ68_000823"
     CDS             complement(17284..17721)
                     /locus_tag="WGQ68_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF723 domain-containing protein"
                     /protein_id="HEZ0714660.1"
                     /translation="MKISIFWLYNAFILERSIMPKSLSLQDVQLRFSSKFPDKTVLKF
                     TKNYEPVTIQCPLHGSVVYGNLQAAMKSSTGCPECTRNPEKPSPNAVAIRLEDTETGE
                     IYEFASTLAASKFIGCSNSTLAVRLSGRTPFDRLIRYRYRLVK"
     gene            complement(18132..19430)
                     /locus_tag="WGQ68_000824"
     CDS             complement(18132..19430)
                     /locus_tag="WGQ68_000824"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEZ0714661.1"
                     /translation="MAKNVVVIGAQWGDEGKGKIVDWLAEEAGGVVRFQGGHNAGHTL
                     VVGGKKTILRLIPSGILHESLDCFIGSGVVVSPEALLGEIDELNAAGVKNVEGRLKIA
                     PTCPLILPYHIALDQAREASRGKGKIGTTGRGIGPAYEDKVARRAIRAADLLHPEKLR
                     EKLDAVLAYYNVQLQHLHNAEPVKAEDVMAVIEKVAPRIAPMITDVSRVLNEKNKNGE
                     KLLFEGAQGALLDIDYGTYPFVTSSNCLAGAASAGAGVGPQMLDYVLGIVKAYTTRVG
                     SGPFPTELFDEVGAGLAERGHEFGSVTGRARRCGWFDAAALKRSIQINGISGMCITKL
                     DVMDGVETINICVGYELPDGGKTDILPCGSDAVETCKPIYETMEGWKESTFGIKNYEE
                     LPDNAKAYLKRIEEVCGAPVAIVSTGPDREETIVLHHPFA"
     gene            complement(19534..20685)
                     /locus_tag="WGQ68_000825"
     CDS             complement(19534..20685)
                     /locus_tag="WGQ68_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0714662.1"
                     /translation="MQTWQLPEHIADVLPTNARQLESAREQLLALFRVHGYELVQPPL
                     MEYAHSLLTHIDAGLSLKTILVTDRLSGRQLGIRADITPQVARIDAHLLSANQGINRL
                     CYAGPVLHAQPDGLLNMREPLQAGAEMYGFADIRGDIELIDLMLKSMKIADMGKVLLS
                     LGHIGIFRALSDAAHLDAGQSATLLALMQDKDTGAVEAQVKAWKLDGMWAKAFSLLPR
                     LYGGREVLSDARGRLPDLSAVGGALGELQAVCDAFPDCEIHIDLSELRVDNYHTGLLY
                     AAYAADFHDAVARGGRYDGLGGYFGRARPATGFSFDLRSFIGRLPAIERQPAVLVDAE
                     DAEAAREAVEALREQGQCVVIDYGIGHNVSEELAGRLKKTDGVWQVVKR"
     gene            21020..22399
                     /gene="norM"
                     /locus_tag="WGQ68_000826"
     CDS             21020..22399
                     /gene="norM"
                     /locus_tag="WGQ68_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter NorM"
                     /protein_id="HEZ0714663.1"
                     /translation="MLLDLNRFSFSVFLKEVRLLTALALPMLLAQVAQVGIGFVDTVM
                     AGGAGKEDLAAVALGSSAFATVYITFMGIMAALNPMIAQLYGAGKTDEVGETGRQGIW
                     FGLFLGVFGMVLMWAAITPFRNWLTLSDYVEGTMAQYMLFTSLAMPAAMVHRALHAYA
                     SSLNRPRLIMLVSFAAFVLNVPLNYIFVYGKFGMPALGGAGCGLATMAVFWFSALALW
                     IYIAKENFFRPFGLTAKFGKPDWAVFKQIWKIGAPIGLSYFLEASAFSFIVFLIAPFG
                     EDYVAAQQVGISLSGILYMIPQSVGSAGTVRIGFSLGRREFSRARYISGVSLVLGWML
                     AVITVLSLVLFRSPLVSMYNNDPAVLSIAATVLLFAGLFQPADFTQCIASYALRGYKV
                     TKVPMFIHAAAFWGCGLLPGYLLAYRFDMGIYGFWTALIASLTIAAIALVWCLELCSR
                     EMVRSHKAV"
     gene            22548..23984
                     /locus_tag="WGQ68_000827"
     CDS             22548..23984
                     /locus_tag="WGQ68_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0714664.1"
                     /translation="MFIKSICLSNFKGFIGDNHQIKFKIPDGATAGSGLNIFIGENNS
                     GKSSIFEAINFLRNGIKEDEAYRIKSKLADDTQPNDACVELAFCGNIQNAITYFVQRN
                     KQAAFNNAINNEQNLKAKRNTVSCKKLDLWDNESQDYSNPSGIDAPFKALFETNFIWA
                     DTNPNDEAAFGSTTLCGLLLKEIAQAHINSAEYQTFRDSFNQVFNSPASNLRQQIATI
                     EQKVQRIFTEQFGQADIHFRFDELKIDSFFKNASIFIDDGVDIPMSEKGNGMQRSVAL
                     ALLQVYAEELAHDEEQGQTKPFYLFIDEPELCLHPKGQTKLLEALLEISRTKQVFLTT
                     HSPYFLVTPHLSNVGLFIFRKDGISNIVEDASLEPMFPWSPTWGEINFKAYKLPTVEL
                     HNELYGKLQHDCGQFREKDFEQWLQSRNIQFTKQWIRENNGSAQAPYDVTLQTFIRNH
                     IHHPENRRNALYTENELRQSIEEMIRLL"
     gene            complement(24221..24420)
                     /locus_tag="WGQ68_000828"
                     /pseudo
     CDS             complement(24221..24420)
                     /locus_tag="WGQ68_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5780 a         5796 c         5865 g         6979 t
ORIGIN      
        1 ccgcctccgc ataaaatcaa aaatgccgtc tgaaaggttc agacggcatt ttgtcaaata
       61 tcggtcccta ttccggcaaa cttctccaca aacgccgcaa tcttttgaaa aacactttgt
      121 ttcttcgtca gatattgcgg gttgagcgga ctcattttcg gcagggtttc ggtcagttcc
      181 gtgccgtttt cgctggcata gccgcgtttg agcgagccgg tcaaatagcg tttggcggcg
      241 gtttcgttca ggccttcggc gacaatcaat cctgccgctt cgtgccgcat cacctcttgc
      301 gcgtaggtgt agaaggtttc cagtatggcg ggaacgtcgg gtactttgtc caaatccgta
      361 tcgttgatga aatccacaat cagaccctct ttggcgcggt ggccgatgct ggcgcggatg
      421 atgcggcgga tttcttccac cagctccgct ttgcctttga tttttttgtg gtgttcgaaa
      481 accagttgca ggatgtaatc cagattgatt tcctgtgatt tgagcaaatc cacctcaaaa
      541 accacatcgt cccaatcgat ttttgactgt tctttctgtt cgcctgcttt ttggcggcgc
      601 aaccaatcgc ggatgtcgtt gtaggcggaa cggtagtcct gcaccgccct ttcagacggt
      661 atcggcactt gccgcatttc ctgcacgtct tcatcgctca agtagtattt ttcttgaaac
      721 gccttcatcg catcttcgtc cgccgcgtcc acactctgca actcgcgcag cgcggcaaat
      781 tcatcgtagt tctgcaatac gttttccgcc cgcaggtatt cgccgaagag tttggcaaaa
      841 tcttttttgt ctttttccgt ttcgatttcg tcgggttcgg ggaaacgttc acgcaactct
      901 tttgccacat ccaaataacc gcgccgcgct tcgccggtct ggctgtcggt atagccgttc
      961 atgtattctt cgtagctttt ttccagcacc acgtttttgg tgtgtttgtc gccgaacagg
     1021 gtaatcgcat caatggttgc ctgctccaaa tcgcggaagc agacaatatt gccgaaggtt
     1081 ttggtggcat cgtaaatgcg gttggtgcgc gaaaacgcct gcatcaggcc gtgatagcgc
     1141 aggtttttat cgacgaacag cgtgttcagc gtcggcgcgt caaaacccgt caaaaacatg
     1201 ccgaccacaa tcagcaaatc cacttcctga tttttcaccc gttttgccaa atcgcggtag
     1261 tagttttgaa aggctttgct gtccgtgccg aaattggttt tgaaacaggc gttgtaatcg
     1321 ttgatggcag cctgcaaaaa ttctttcgcg ctgccgtcca tcgcttccgg ttcaaaggtc
     1381 tcatcaacaa tttcaccgac ggcgttctgc tcttcgttgg ccgcaaagga aaaaatggtg
     1441 gccactttca gcgggtgcag gctgcctgcc tgttgtgttt tgaacgcttc gtaatagcac
     1501 ttcgccgcat ccacgctgct gaccgcaaac atggcgttga agcctttacc gcccgcattc
     1561 aggcggtgcg ttttctgcct gaactggttc aggatatatt gcgtgatttc gcggatgcgt
     1621 tcggggtgca gcagggcttg gcggttttcg gcagcactca gtttcttctc atcctgttcc
     1681 gcttccacgg ctttgaactg cgggcgcaca tcgttgtaat ccactttgaa tttcaatact
     1741 ttttcatcgc ggatggcatc ggtaatcaca taagaatgca actcacgccc gaacacgccc
     1801 gccgtggttt ccgcgcccaa agcgttttcg ggaaaaatcg gcgtaccggt aaagccgaac
     1861 tggcagaatt ttttaaattt ctttttcaga tttttttgcg cttcgccgaa ttgcgagcgg
     1921 tggcattcgt cgaaaataaa gacaacctgc tggtgataaa ccggcagatt atcctcactc
     1981 ttcatcaggt tgttcagctt ttggatggtg gtaacgatga ttttgttgtc gtctttttcc
     2041 aaattgcgtt tcaaacccgc cgtgctttcc gaaccgttta cgctgtcggg cgaaaaacgc
     2101 tggtattcct tcatcgtctg gtagtccaaa tccttcctgt ccaccacgaa gaaaaccttg
     2161 tcgataaacg ccgattccgt cgccagacgc gccgccttaa agctggtcag cgttttgccg
     2221 ctgcccgtgg tatgccaaac atagccgccg ctttccggtt tgctccaatt cttcgcctgc
     2281 gccgaactgt taattttcca cagaatgcgt tcggcggcgg caatctggta cggccgcata
     2341 atcagcagcg tatcattcgc atcgaacacg ctgtaatgca gcaaaacgct cagtaatacg
     2401 ctttttttgc aggaacgtgg cggtaaagtc tttcaaatcc ttaatcggat gattgtccga
     2461 ccgcgcccaa ttcatcgtga aatcgaagct gtttttgtcg cgcttggtgg tattggcgaa
     2521 atagcgcgtg tccgtgccgt tggaaatcac gaagatttgc aggaatttga acagcgaatt
     2581 ttcgctgttg aagctctctt tgctgtaacg gtgcacctga ttgaatgcct cgcgcaccgc
     2641 cacaccgcgc tttttcaatt caatctgcac cagcggcagg ccgtttacca acacggtaac
     2701 gtcatagcgg tttgcatgcg tgcccgtctg ctcaaactgg ttgataagct gcacatggtt
     2761 gcgggcaagg tttttcttgt ccagcagata aatgtttttc agacggccgt catcaaaagc
     2821 gaaatcatag atatggtcgt cgtggatttt gcgggtttta tcggtaatgt tttcagacgg
     2881 cctgtccaga tattccgtca aaaaccgcgc ccattcgccg tctgaaaacg ccacatcgtt
     2941 cagccgctgc aactgcgcgc gcaggttttc cagcagcctg ctttggctgt tcaaatcctt
     3001 gcggtattcg taaccctgat tctgcaaatc ggcgattaac tccgcttcca actggttttc
     3061 cgattggtag ctgcccgact gttcgatttt ttcatattgg tcgagcacga tgaaattcgg
     3121 cgtttcggcg atgggtttgg tttcgaggtt catatgggtc gtcctttgcg gtgtcgggtt
     3181 ttatagtgaa ttaaatttaa accggtaccg cgttggctcg ccttgccgta ctatttgtac
     3241 tgtctgcggc tcgccgcctt gtcctgattt aaatttaatt cactataaac ggtttgatgt
     3301 tcggattggg ctgcctgaaa atgtgcaggc tgctttttca ggtagcctta gggaaggcaa
     3361 gcagctgttc gcggtaatat tcgtattgtt tgcggcgcag ggcgatttcg tgcggcaggc
     3421 cttcgctgat agagtgggtc agggtatcga atttgtctag gatggcggca attttttcct
     3481 gttcggggag tggtgggatg gggattttta atttctttaa ttctttcatg ctaataatag
     3541 gtaaacttga agtattaata agttttttgc tctgctccgc aataatgaaa aaataataga
     3601 aagcgaaatt tatatctaaa gcatccttaa attcatcttt aattgtaaaa ttggtcagtt
     3661 gttggttact aatataatta acttttatta acgcatgctc tcctatggtt gccgttgtcg
     3721 acatcattat tgatttagca ggaaacagct tgccgccctt aactgcgctt tttgatatat
     3781 gcttcaatga attatctaaa atacggccat tttccctaat atcttccatt ctaaaccaag
     3841 ggattgagcc attttcccaa tattctttat ttgatttgga aggggtatag ccattcttca
     3901 agtcaaacac ctcccccaac gtcttccaaa ccacatcttt cagacggcct ttatagccat
     3961 cagctatccc cccgatttga ttgtcaaaat ctaaaagaaa gtcgcggtaa taccggtatt
     4021 ggcgtttgcg cagggctaat tcagcttcca gcttggtgaa tttgtcaagt atttttacaa
     4081 ttttttgttg ggtttccagg ggtgggatag ggattaataa ttttttcaag ttactggcat
     4141 ttagagcagg aatacctgta aatgtacata aggcatgaat ctcttgttca aattcaagta
     4201 atatatgata gagaaaacga acatccaatt ctgttctatt cttaatagta actgcataat
     4261 ttaagttgcc acgaaaatat cttccttctt gccacgttat tgagccgact cccgctcctc
     4321 ttgttgtgat gccgataggg tcattttcag tgttccattc atcaatatag ccaagcggct
     4381 ctttgccact gttaataact ggataactac caatattatt tgagattttc tgcttactta
     4441 cagcttgtcc tgtttttatg atagctatcc cattttctcc acccaacggc ttccactcca
     4501 ccggtgccgt ctgaatcatc tcaatcaatt ttttcgcttt gctttgcata tccatggttg
     4561 ttttccgttt ctttcccgct gtggcgggat gtggggcggt tgtggggtgc ttcggctcac
     4621 tctgtccgtt tgccgttatt tctgcgggct gaagtccgtc ctgctttcag gctgcctgca
     4681 atggtgtgca ggctgctttt tcaggtagcc tgaatggtag ttattttgtg cggtcttttt
     4741 tctgtttgat ctgttgcacg gctgtttttt ccaacgcttt aacgctttcc agataggctt
     4801 gctccaccgg cgacaggatg cgcgcttcat aggcgcggta ttcgcgttcg gctttctgct
     4861 ctgcctgttt gcggctgatg ctgcctgcac cctgtaatgt gccttgtccg tacagcccgg
     4921 aaaatttgtc caattcggct atccagtcgc gcatatacat ggggctttgc tcctgcgctt
     4981 tgatttccgc caggtcgaag aaggcggaaa ccagacggtt caggcggaac agttcgtctt
     5041 cggtcagata gtttttggcg attttggctt cattcagcgt ggggattgcg ccttgaaagg
     5101 tggtcagccc cataaagtct ttgctgctgt cggcacggct gtatatcagc tcagctacgg
     5161 tttgccggct ggcggcatag tgcagtttgt tttgaacggc ggcaaaaaag gtttggcttt
     5221 cgctgctttt ggggttgtag tcttggctgg tggcatataa atcaagcact tgccggtata
     5281 gggctttttc gctgctgcgg atgtcgcgaa tgcggttgag cagttctttc caatagtcgc
     5341 cgccgcctgt gcctttcagg cgttcgtcgt ctatggcaaa gcctttggtc agatattcgt
     5401 ccagccgttc ggttgcccat tggcggaatt ggatgccccg cgcggaacgg acgcggtagc
     5461 cgacggcaat aatcatgggc agggaataat gggcgatttt gcggtttacc ttgcgcccgt
     5521 tttcattttg aactgtcaac tggaagttga cagttgcctt ctcttccaat tcttgctctg
     5581 caagaatggt tttaatgtgt ttgctgatat tttgtttggt ggtttggtac agttctgcca
     5641 tgtccgcctg cgtcagccaa agctgtccgc caaattcctg caaggcaaat tgggcagtgc
     5701 cgtcgttggc ggtgtatagg atgatgctca tgcttcaatc tctgcactca cttcgtcaat
     5761 ttcacgccgc agccgttcga ttttggcaac ggtttcgctg atttcggcgt taagctgttt
     5821 gatgtcgata atttcgcggg tgtcttcggg ttcaacatag ctgctgacgg cgaggttgta
     5881 gccgttgtct ttgacggttt gctgggcggc gttttgggcg atatgcggca catcggcttt
     5941 atcggcgaag agtttgacga tttcggcaat gtgttcttcg gttaagacgt tgttgttggt
     6001 ttctttttta aagaagccgc ctgcgtcgat gaattggatg tcggtattgt ctttgtgttt
     6061 ggacaaaacc agtatattga cggcgatgcc ggtgccgtaa aagagattgg gcgcaagggc
     6121 gatgacggtt tccacgtagt tgccctccac cagatattgg cggattttct gctctgcgcc
     6181 gccgcgatag aaaatgccgg ggaatgagac gatggcggcg cggcctctgc cagaaaggta
     6241 gttcagtgcg tgcagaatga aggcaaaatc ggctttggat ttcggggcga gtacgcctgc
     6301 aggggcaaag cggtcgtcgt tgattaaggt ggggtcgccg ctgcctatcc agttgatgga
     6361 atagggcgga ttggaaacga cggcatcaaa gggtttgctg tctttgagtt tgggattggt
     6421 cagtgtgtcg cccaattcga tgtggaattt gttgtaattg acgttgtgca gaaacatatt
     6481 catgcgggcg aggttgtagg tggtgtggtt gatttcctgc ccgaagaagc cttcttcgat
     6541 gatgtgctcg tcaaactgtt ttttcgcctg caagagcagg ctgcccgagc cgcaagctgg
     6601 gtcgtagatt ttgtttactt tctcctgccc gtgcaccgcc agccgcgcaa tcagcttgga
     6661 tacgctttgc ggggtgaaaa attcgccgcc ggatttgcct gcgttggcag cgtagttgga
     6721 aatcaggtat tcgtaggcat cgccgaaaag gtcgatgtgg tggtcttcaa aactgccgaa
     6781 atcgagttcc gccacgcctt ttaggacggc ggcaaggcgt ttgttcttgt cggcaacggt
     6841 gctgccgagc cggctgctgg tggtgtcgaa gtcgtcaaac aggcctttaa tgtcttgttc
     6901 ggacggatag ccggaggcgg agctttcaat cgcggtaaaa atttctttca gcttggtgtt
     6961 gagctcttcg ttttgatggg cttcggcggc aatattgcaa aaaagctggc cggggtagat
     7021 gaaatagcct ttgactttga cggcatcgtc tttgatttcg ggcgtgatga tgctgtccgg
     7081 catagcggcg taatcaatac tgctgtcgcc tgcctgcata tagtcggtaa agttttcgct
     7141 gataaagcgg tagaaaagtg tgccgagaac gtattgttta aagtcccagc cgtccaccgc
     7201 gccgcgtact tcgtcggcaa ttttccaaat ttggcggtgc agttgggcgc gttgttatat
     7261 ttcggtcatc atcgaaatcc atataaaagt taaacaaatc gaaatcgcct gatattttca
     7321 gacgattttt tacgggcatt caaattaagc caaattttcc agcatccatt tgacgtagcg
     7381 ctcgacgcct gatttgacat cgaaaaattc ttccgtataa cctgcctcac gcaatttggt
     7441 gatgtcggct tgagtgaagc tttggtattt gcctttgagc gcatcgggaa atgggatgta
     7501 gcggataagt tcttcttcta ccagctcttt caagctcatt tcgggtttgc cttcggcggc
     7561 gcggcaggcg ttgacggtgg cggcagcgag ttcgttgaac tgttggctgc ggcctgttcc
     7621 gaggttgtag atgccggaaa gttcgggatg gtcgaagaag tagaggttga ctttggcaac
     7681 gtcttcgacg ctgacgaagt cgcgggtttg ttcgccgttg ccgtagccgt cgttgctgcc
     7741 gaacaggttg acgtaaccgt gttcgcggta ttggtggaag tggtggaagg cgacggatgc
     7801 catgcgacct ttgtgttgtt cgtgttgtcc gtaaacgttg aagtagcgga agccgacgac
     7861 ttgggcggtg agaccttctt tcatgcggcg gcgcaatact tggtcaaaca ggaatttgga
     7921 gtagccgtac acgttgagcg gtttttcgag ttcgcgctct tcgcggaaga tttcgccttt
     7981 gccgtaaacc gccgcactgg aggcataaag gaagggaatg cgttcgtcct gacaccagtc
     8041 caacagatcc agcgtgtact ggtagttgtt gtccatcata tacaaaccgt cgtggttcat
     8101 cgtatcggaa cacgcgcctt gatggaaaac ggcttcgatg ttttgataag gtaaaatgtg
     8161 ttccctcact tggcggatga attcatgttt gtcgagataa tgggcgattt cgcactcggc
     8221 aaggtttttg aatttttcgc ctttgctcaa attatcgacg gcaacaatgt cagtaatacc
     8281 gcgttgatta agtgctttga cgatgttgct gccgataaag ccggccgcgc ctgttacgat
     8341 gatggtcata ttcggtttcc tttgttttga gtaaaattaa taaacttagt tacaagaaat
     8401 catcgatatt gtcctgaaac acaaatttgc gatgatattc caaatatttt tctcttcctt
     8461 cgggatatat tctcatctta ttttttgcat ttggattgat attaagtttt tcccatgtgt
     8521 aacggcttag tcgagtaccg caaatcaaac gaccatcatc caagaaagta atataacctt
     8581 gattgaacaa ccaatcataa gtcggcgtta aagctaaccc atttaaataa tctaatgcct
     8641 ctttctcatt tttctcagta atacaaacca tataaggttt aatatgacta gcccttaaca
     8701 aaatctcatc tgtaattaat gtaaatggac attgcggcat atgatttata acatccttgc
     8761 gatatttctg ctgccctttt ctacttttct gtttaatgat ttcttgctct acgaattgat
     8821 taaattctga agccggtatt tctaattttt ctcctgataa gagttgcggg tgcacgatag
     8881 agcgaaattg ataatccaaa aatattcgga aataaaataa tggttctgaa tcttctatat
     8941 ctttgacagg caatagttta agaattgata aataactaat tttgggcaat acaatcttac
     9001 gccaaattga ccatatatca tcctccgaac ggatataacc cctacggtaa cctttagaat
     9061 tttccaaatg gtctgataca tcatggattg aaaagaaaat attttctgta ggcagattat
     9121 taatacttaa aacgtaatca tcataaaacc ttgaaatatc atgatgatat ttctgttctt
     9181 gtttgtgata ttcaactttt gcatcaacca aataacgctg taaatttttc ttattaaaaa
     9241 aacaattttt ctgaatgaga ggatattcta ccctccttcc attttttgtc tcagtaatcc
     9301 cccaattttc atactcaaaa aatgtactta gttttcgacc actatcatca atataactac
     9361 ctacatattt gcgtgcttcc ccattaccac caaaaacaga taacttgtta ttcctgtgaa
     9421 taaaactatc ttccgcacgt aaattttgaa tactatcgac aataaaatac tctatgtttt
     9481 caatagtaat tctttgaaaa tccggcattt tactctccat aaagcgtaat atccatttgt
     9541 tttaatatag cgattaatac atcaacaaca atactgtttc ccgcttggcg atacatttga
     9601 gtatcactaa ctaaaatttt aaaatcatct ctaaaaccca ttagccgcaa acattcccta
     9661 ggtgttaatt tacgaatacg tccacgatta tgagtaacat aattatcaac cccggcacga
     9721 tgcattttat gcatagtttg caataatggg cgagctacag gcaaatcagt ttccgtactg
     9781 gttttaaaat tttttgttcc tcctgccaaa acataatttt tgattttttc agaaagataa
     9841 tatttttcct caacgttatt tacatcaaaa ataaagtcat caaattcaga ttcgcgggct
     9901 gcctgaaaaa taaaatcacc atgccaatta aattgttgat ttgctttttg gcataaggca
     9961 atttctccat taatctgtgt ataacgtttt tgtctatttt tagaactggt aacaaatttc
    10021 acaccctttt cacgtaaaaa atatttgcta tcagtatagt cctctaaaaa atcttgcata
    10081 gtatgttcta attcaatctt ttcaggaaac tgaaaaccat ttataggagg ggtatgaaag
    10141 ccaacaacaa aaatacgctc acgatgttga ggaatcccat aatccttact attcattatt
    10201 tggaaatata agtcataacc aagtgaataa aaaacacttt ttacaacttt ccaagttttt
    10261 ccattatcat gattgagcaa gccctttaca ttttcataaa taaatatctt tggttggact
    10321 tcatccacaa cgcgagcaaa ttcatagaat aatgttcctc gtgtatcttc taatcctgca
    10381 cgtttgccaa ccatggaaaa tgcttggcaa ggactgcctc caactaaaat atcaacttga
    10441 tttctaaact tgctcgcatc aaattgagta atgtcgttat gccaaaaatc ttcatttaat
    10501 ttatagtttc caagataact ttttttaacg tatggatcaa tatctcctgc aaaaacaatg
    10561 gtatggttta aatttaatcg gtggaatgcc tgttcaaccg caccaatccc gctgaatacg
    10621 gttgctaatc taatatgtga atcaggttta agaaaagttt tagatttcca accttgttga
    10681 ctgggaaaga gcaaagtttt ttgtaatcga gtatcgtgtg tctgtgccat tgtcgaaata
    10741 gtcatactta tatcgttctg tttatcttat caatatgaaa actacattgt tgattgccct
    10801 gacaatgcct tggtcaattc ggcaaacgag caaaccgccg taccgagttt cgccacgaca
    10861 accccggccg cagtattggc aaggtacatg gcttcgggca tggtgcagcc tgccgccaaa
    10921 cccaagccca ttccggcaat gacggtgtcg cccgcaccgg atacgtcgta aacttcttgg
    10981 gcgcgggtgg gctggtaaat cggttcgcct tcactgaaca aggtcatgcc ttcttcgctt
    11041 cgggtcagta aaacggcggt caggtcgagg tggcggcgca ggttttgcgc tttttcggtc
    11101 agctcgcctt cgtttttcca actgccgacc acttctttca attcggcgcg gttgggcgta
    11161 atcagggttg cgccggcgta tttttcgtaa tcatcgcctt tggggtcgat taatacgatt
    11221 ttgccttcgt gtttcgcaca atcgatcata tcggagatgt gcgacaggcc gcctttgccg
    11281 tagtctgaaa aaataattgc atcgtattcg gacaatactt cgaggtattt ctgcttgatt
    11341 tgttccaata cttcgcggtt gggatgttct tcaaaatcaa ggcggataag ctgctggttg
    11401 cgggcgacga cgcgcagttt gacggtggtg gcgatttgct tgtcgcgcat cagataggag
    11461 gcgacgccgt cttgtaccat cagcgcgtcg agcgcgtcgg cggcttcgtc gtcgccggtt
    11521 accgacaaca gccctgccct gccgcccaac gaagcgatgt tgcgcgcaac gttcgccgca
    11581 ccgcccgcgc gttggtcgat acgtccgatt tttgctacgg gaacaggggc ttcgggcgaa
    11641 atacgggaca catcgccaaa ccaatagcgg tcgagcatca cgtcgccaac aaccaggact
    11701 ttggcttgtg cgaaacggga tttgagggtt tcttgttgaa acttggcggg catattgcct
    11761 ccgttgtgtt ttgttcagac gaccttaacc ggtaaggccg cctgaaaatc agtttgcttc
    11821 aaggtttgcc acgcggttca cttcgcgcaa tacggctagc ggattggcgg cttgggtaac
    11881 ggggcggccc atcacgagat aagtcgaacc tgccgccaag gcttcggcgg gcgtcatgat
    11941 gcggcgttgg tcgtcgttgt tgccggcaac gtccaagcgg ataccgggcg tgaccaagac
    12001 aaaatcttgt cccaactcgc ggcgcagcgg tgcggcttct tgggcggagc agaccacgcc
    12061 gtccaagccc gaactttgcg ccagtttcgc caaacggatg acttgttctt cgggagcgat
    12121 gttcaaaccg atttccgcca aatcgctttg ttccatgctg gtcaacacgg ttacgccgat
    12181 taagagcggc tttgtgccgt atccggcaac ggcttctgct gcggcttcca tcatacggcg
    12241 gccgcccgac gcgtgcatat cgaccatcca aacgcccatg tcggcagcga ctttgcaggc
    12301 ttgcgcgacg gtgtggggaa tatcgtggta tttcaaatcg aggaaaagtt tgaaaccttg
    12361 attgattaag ctttctgcca aactgcgtcc cgtcgcggta aacagctctt tgccgatttt
    12421 gatttgacac aatgtcgggt caaggttgcg gacgaatccg agcgtgtctt tttcgttggc
    12481 aaaatcaagg gcgacgatga cgggggtgcg ttgttgcgga gtttggaagt cggagattaa
    12541 aggattcatg gtgttttcgt ttcgggtttt cagacgacct ttatcgtctt tttcgcagta
    12601 tagtggatta actttaaacc ggtacggcgt tgtctcgcct tagttcaaag agaacgcttc
    12661 tctaaggtgc tgaagcacaa agtgaatcgg ttccgtacta tctgtactgc ctgcggcttc
    12721 gtcgccttgt cctgattttt gttaatccac tataacaacg taaattcaaa ccgtaggtcg
    12781 ggcattgatg tccgacctac gcggactcaa gcctccgaca aactgtttcg gataaatttg
    12841 aaccggtaca tcagccgtca ttcccgcgca ggcgggaatc cattgataaa actcagttca
    12901 tcagatttaa acagcgattg cccaaattca gaaatggatt cccgcctgcg cgggaatgac
    12961 gccaaatgat tatttttcgg ttgcaacacg ctgtttatca aaaacaggtc gtctgaaaac
    13021 ctgattttct gttttcagag tgcgaagcct gcccgtgggt acgacctttt cgatttattt
    13081 gcccaatgcg cccaatactt cggctttcac ggcttctacc ggctgagtgc cgtcaacttt
    13141 gatgtattta ggcgcgtgtt cgccttccag tttgctgtaa aaatcgacca aaacttcggt
    13201 ttgctcgtgg taaacggcaa ggcgtttttt cacggtttct tctttgtcgt cgtcgcgctg
    13261 aatcaaatct tcgccggtta cgtcgtcttt gccttcaact ttgggcgggt tgtaggtaac
    13321 gtggtaagta cggccggaag ccaaatgcac gcggcggccg ctcatacggt cgacaatcac
    13381 gctgtcaggc acgtcgattt caacgactgc atccaaatcc acgcctgctt caaccatcgc
    13441 ttcggcttgt gccaatgtgc gcgggaaacc gtcgaacagg aaaccgtttt tgcagtcgtc
    13501 ttgcgcgatg cgttctttga ccatgccgat aatgatgtcg tcgcgcacca agccgccttc
    13561 gtcaatgatt tttttcgctt ccaaacccaa gggcgtgcca gccttaatcg cggcgcggag
    13621 catgtcgccg gtagagattt gcggaatgcc gaacgcggcg gtgatgaatt gcgcctgagt
    13681 gcctttgccc gcgcccggcg cgcctaaaag taatgctttc atgaagtgat cctttgaaat
    13741 gtgtttattg gaatcaaaca gatggtttac gattctacac ggcttggcac aatagttcaa
    13801 aaaccaactg cacaaactgc catttgcaag caaatgtttt gcataccttt gccgagtctg
    13861 atattcacaa gccggcaact ttttgggaca atccccaaaa tttcaaaaca tcacacccta
    13921 ccaaaaagga acatccgtga aacgaatttt tctgtttttg gctaccaata tcgctgtttt
    13981 ggtcgtaatc aacattgttt tggcggtttt gggcatcaac agccggggcg gcgcgggcag
    14041 cctgttggcg tattccgccg tcgtcggctt cactggttcg attatttcgc tgctgatgtc
    14101 caaattcatc gccaaacaat cggtcggcgc ggaagttatc gacacgccgc gcaccgaaga
    14161 agaagcctgg cttttgaaca ctgtcgaagc ccaagcgcgg caatggaacc tgaaaacacc
    14221 cgaggtcgcc atctaccact cccccgaacc caatgccttt gccacgggcg catcgagaaa
    14281 cagctccctg atcgccgtca gcaccggttt gctcgaccat atgacgcgcg acgaagtgga
    14341 agccgtattg gcgcacgaaa tggcacacgt cggcaacggc gatatggtta cgctgacgct
    14401 gattcaaggc gtggtcaata cctttgtcgt gttcctgtcg cgcattattg ccaacctgat
    14461 tgcccgaaac aacgacggca gccagtccca gggaacttat ttcctggtca gcatggtatt
    14521 ccaaatcctg ttcggcttcc ttgccagctt aattgtcatg tggttcagcc gacaacgcga
    14581 ataccgcgcc gatgcgggcg cggcaaaact ggtcggcgcg ccgaaaatga tttccgccct
    14641 gcaaaggctc aaaggcaacc cggtcgattt gcccgaagaa atgaacgcaa tgggcatcgc
    14701 cggagatacg cgcgactccc tgctcagcac ccacccttcg ctggacaacc gaatcgcccg
    14761 cctcaaatcg ctttaaaccg atttgaaacg gcaaaaaccg tacgcgcaag cagtacggtt
    14821 ttttgtatat gctgtctgaa aacatcaggt ttccataccg acaccaaccg tatccatttc
    14881 cgcaccggat acgccacatt gatataccct gataaaccgt atccggacag aaaatatagt
    14941 ggattaacaa aaaccagtac ggcgttgcct cgccttagct caaagaaaac gattctctaa
    15001 ggtgctgaag caccaagtga atcggttccg tactatttat actgtctgcg gcttcgtcgc
    15061 cttgtcctga tttttgttaa tcccctatat cagataaaac agcggtaaaa ttgccgtctg
    15121 aaaccaactc tgcgccatac gggacacgct ctgcacgtct atttgcctcc gatatatcct
    15181 ccctactgta ggaacgtctg ccgatttgca cgaaatcggc acctctagca cccttcagga
    15241 tggcggacgt tatccgttct tgggattctg caagacggtc gacggcatta tacagacgct
    15301 cggtctgata cccttccgaa gcaacctgct tcaggtattc caaataaccg taacccaacg
    15361 cccctccgac aagcagggtc gtcaacgcta ccagcgttgc cgaacggatt ttagggggca
    15421 tatcttgaag catatctcta ccgaaccgaa ccaaagtctc tttaaagtcg gactctacac
    15481 gcgtagaacc ctcggaaatc ttgaagttca actcatactg cttccgtttg tccgctgtca
    15541 accgcttgag gttgtcatcc ccgtattgga cggttgacac aaccctgtac agactgtcct
    15601 gtatttcgac cagcgtctgt gcaaagttgg cggtcataac tggattccca acccttgccg
    15661 ccgattctga tactcaaaac ggtatccagg cgttccagca gggcttcggg agacagcagt
    15721 gcgtattctt caatattttc cataggttga ttatagcaga accttatacc tgttacagac
    15781 aaggttgtcg ggatttgaac gtccgtcgag cttggttcgt atagagccgt agggggtgtt
    15841 tagggctttg gcggcagctt gtacgctggg gaacgtgagt gtttcgcctg tggcggtgtc
    15901 gaggatggag acaggttttt ggttttgggg aggcgtattt tccttcattt taagtttccc
    15961 gcactccgga cacccatact ttgactttat agctgtgtcg taatacaaaa gttggacaac
    16021 cccgtgcgta ggacatctaa cactggaagg tttataaatg ccactgaact ccaacaattc
    16081 taaatgtgga aatttctctt gaaagcgtga tttggcggta tcgaaagtca ttcgcttacc
    16141 cataatttgt ctccataggt tatgtgcaaa tggattacac aaccaaaata tagaaatttt
    16201 caaatttttt gagacctacc tgaaatcctg cgcaagcaca atgtaaagaa aaatggtata
    16261 cgtctttact aaatctttgc ataaattaac tctaaccggg atttgaacgt ccgtcgagct
    16321 tggttcgtag gatttagggt gttgtgaacc ttacctatcg tacccaagca ctttgtacct
    16381 gtttgaaata aggttgtcgg gcgacgttcg acccttgagc ctgtgattga tttgttggaa
    16441 atgtacacct aaagcagcac ctgcggcagt aacgcttggg aacgtgagtg tttcgttggt
    16501 cgttgtgtcg aggatgagga ggggcttccc tacatttgga ggtgtttggg tttttgacat
    16561 cttagcacac tcaggacacc cgtactttga ctttataagg gcattaaatg tcgaataacg
    16621 aacgttcccg tgcataggac aattaacggt tactgggtaa ttcacaccgt caaagccggt
    16681 tagagagata ttagggaata tatcagtaag ttcagaagct gcttggttga aagatttggc
    16741 catttgagac tccaaaattg attgtcaaat ggatttaata gcaaatttat aaaaatgtca
    16801 aatttttcga gaagcacctg atagtggcga ggggtataca ctgttaaaaa gtgtatacgt
    16861 catttagtgc agatgtgaac cttatacctc cctgcaagta aagtctctgt gtgaacccga
    16921 ccttttatac ggtcagtgat agctgctggg tttataccca atgaccttgc agcagatgct
    16981 cttgagggga atgtaagtgt ttcgccagtt acagtatcct ccaatactgt atttttagat
    17041 aatttatcta ttgtatcttc tggaattttg tgggaattga caccataaga tgcacatttt
    17101 ggacacccac ttttagagtc caacatactt ttgaatgtag agcaagtaac cctcccgtgt
    17161 ataggacaga cgattaccgt cgggtaacga acgccattga actccacaag attcaaatgt
    17221 ggaaatttag aggttagctt agaaactgct tgagtaaatg taagtgccat agtggttgtg
    17281 aacttacttt accaacctat aacgatacct aatcaatcgg tcaaacggag tgcgtccgct
    17341 taaacgtaca gctaaagtgc tgttggagca accgataaat ttactggcag ccagtgtact
    17401 tgcaaactcg tagatttccc ctgtctctgt gtcctccaac ctaatggcaa cggcgtttgg
    17461 agagggcttt tcgggatttc gagtacattc aggacatcct gttgaggatt tcatggcagc
    17521 ttgtagattc ccgtaaacaa ccgacccatg caaagggcat tggattgtta ccggttcgta
    17581 gtttttagtg aacttcaaaa ccgtcttatc aggaaattta ctggaaaacc ttaactgaac
    17641 atcttgtagc gagagtgatt ttggcattat agacctttct aaaatgaaag cattatacaa
    17701 ccaaaatata gaaattttca aattttctct gaacgcactg gtatccgatg gagctaaatg
    17761 taaagaaaag ggtatatgtc cttactaaat ctttacacat tttaactgta tagtgtttga
    17821 caaacaacag gcataggcgc ataatgcacc cgtagttaga tagatagtaa ggagataacc
    17881 aatgacttag cacctgcccc cctctctctc gcgcaccccg cccctcgctt gcggctaatg
    17941 ggctttgcgc gaaatataca ctttcatctc ctgcatggct taaccgccta tgcaatagta
    18001 cacacatcaa ccaatcaggc tggcacgtct tggcattggc tgacgtacca ttgtagcccg
    18061 aaatgttgat accctactat ttatgaccaa cggcggatta gggcttcaga cggcaatttt
    18121 actgcaaaac cttatgcgaa cggatgatgc agcacaatcg tttcttcgcg gtcggggccg
    18181 gtggagacga tggcgaccgg cgcgccgcag acttcttcaa tccgtttcaa atatgctttg
    18241 gcattatcgg gcagttcctc ataatttttg atgccgaaag tcgattcttt ccaaccctcc
    18301 attgtttcgt aaatcggttt gcaggtttcc accgcatccg aaccgcaagg caggatatcg
    18361 gttttgccgc cgtcgggcaa ttcgtagccg acgcagatat tgatggtttc aacgccgtcc
    18421 attacatcga gtttggtaat gcacataccg gaaatgccgt tgatttggat ggagcgtttc
    18481 agggcggcgg catcaaacca gccgcagcgg cgtgcgcggc cggttaccga gccgaattcg
    18541 tgtccgcgtt ccgccaaacc tgcgcctact tcgtcgaaca attcggtcgg gaacgggcct
    18601 gaaccgacgc gcgtggtata ggctttgacg atgcccaaaa cataatccag catttgagga
    18661 cctacgcccg cgcctgcgga agcggctccc gccagacagt tggacgaggt aacgaaggga
    18721 taagtgccgt agtcgatgtc caacaacgca ccttgcgcgc cttcaaacag cagtttttcg
    18781 ccgtttttgt ttttctcgtt caacacgcgg gacacgtcgg taatcatcgg cgcaatgcgc
    18841 ggcgcgactt tttcgataac cgccatcacg tcttccgctt taaccggctc ggcattgtgc
    18901 agatgttgca gttggacatt gtaataggca aggacggcat ccagtttttc acgcagtttt
    18961 tcaggatgca gcaaatcggc ggcgcgaatg gcgcggcgtg ccactttgtc ttcgtaggca
    19021 gggccgatgc cgcggccggt cgtgccgatt ttgcctttgc cgcgcgatgc ttcgcgggct
    19081 tggtcgagcg cgatgtggta aggcaggatc agcgggcagg tcggcgcgat tttcagacgg
    19141 ccttcgacgt ttttcacgcc tgccgcgttc aactcgtcga tttcgcccaa cagggcttcg
    19201 ggggagacga caacgcccga accgatgaag cagtccaaac tttcatgcag gatgccgctc
    19261 ggaatcaggc gcaaaatggt ttttttgccg ccgacaacca aggtatggcc cgcattgtgg
    19321 ccgccttgga agcgcaccac gccgccggct tcttccgcca gccagtcaac gattttacct
    19381 ttaccctcgt cgccccactg tgcgccgatt actacaacat ttttagccat agccatataa
    19441 cctatcgata ttaaaaatta ttgcggaaat ccccaaaccg gtgcggcttt gcgccccgcc
    19501 acgatttgcc tttcatccgc catgaacggg tatttagcgt ttcacgacct gccaaacgcc
    19561 gtccgtcttt ttcagacggc ctgcaagctc ttccgaaaca ttgtgtccga taccgtaatc
    19621 gattacgaca cactgccctt gttcacgcaa ggcttcgacc gcttcgcgcg ccgcttcggc
    19681 atcttccgca tcgaccaaca cggcgggctg ccgttcgatg gcgggcaaac gcccgataaa
    19741 gctgcgcaag tcgaaactga atcccgttgc cgggcgcgcc ctgccgaaat atccgcccaa
    19801 tccgtcataa cgcccgccgc gcgcgaccgc gtcgtggaaa tcggcggcat aggcggcata
    19861 cagcaagccc gtgtggtaat tgtcgacacg cagctcggac aagtcgatat ggatttcaca
    19921 atcggggaat gcgtcgcaca ccgcctgcaa ttcgcccaac gcgccgccga ccgccgacaa
    19981 atccggcaac cgtccgcgcg cgtcggacaa cacttcacgc ccgccgtaca ggcgcggcaa
    20041 cagcgagaat gcttttgccc acatgccgtc cagcttccaa gccttgacct gcgcttcgac
    20101 cgccccggta tctttatcct gcatcaaggc aagcagcgtt gcggactgcc ccgcatccaa
    20161 atgcgccgca tcggacaagg cgcggaatat gccgatatgc cccagcgaaa gcagcacttt
    20221 gcccatatcg gcaattttca tgcttttcag catcaggtct atcagctcga tgtcgccacg
    20281 gatgtcggca aaaccgtaca tttccgcccc tgcctgcaag ggttcgcgca tattcagcag
    20341 accgtcgggc tgcgcgtgca acaccggacc ggcataacac aaccggttaa tcccttggtt
    20401 ggcggataaa agatgggcat caatacgcgc cacctgcggc gtgatgtcgg cgcgtatgcc
    20461 caactgcctg ccgctgagcc tgtccgttac caaaatggtt ttcagggaaa gccccgcatc
    20521 gatatgcgtc agcagggaat gtgcgtactc catcagcgga ggctgtacca gttcataacc
    20581 gtgtacgcgg aacagtgcca acaactgctc cctcgcgctt tcaagctgcc gcgcgttcgt
    20641 gggcagtacg tcggcgatat gttccggaag ctgccatgtc tgcatgagaa tctgccttct
    20701 attttatcat gtaaatacaa aaggctgccc gatccgcaat cagacggcat ctttgccgca
    20761 acgcatcacg ccgctgtcaa aacggcgatg cttccttccg attcagaatc cgaacagccc
    20821 ctgtccgacg gctcaatgcc gtcaagtcgt taaaaccaaa ctttaccata aaatacacac
    20881 aatctgaata tacttttata ccctgtatcc gccgtctgac ggcacggcat ttttttacta
    20941 tataatgtgc gccattattt ctcatcactt cctgctgccg ttttctgctt cagacggcat
    21001 ttttgttgat tgaacactta tgctgctcga cctcaaccgc ttttcctttt ccgtcttcct
    21061 gaaagaagtc cgcctgctga ccgctcttgc cctgcccatg ctgttggcgc aggtcgcgca
    21121 ggtgggcatc ggttttgtcg ataccgtgat ggcgggcggt gcgggcaagg aagacttggc
    21181 ggcggtggct ttgggcagca gcgcgtttgc cacggtttat attaccttta tgggcattat
    21241 ggcggcgctg aacccgatga ttgcccagct ttacggcgcg ggtaaaaccg acgaagtggg
    21301 cgaaacggga cggcagggga tttggttcgg gctgtttttg ggcgtgttcg gcatggtctt
    21361 gatgtgggcg gcgattacgc cgttccgcaa ctggctgacc ttgagcgatt atgtggaagg
    21421 cacaatggcg cagtatatgc tgttcaccag cttggcgatg ccggcggcaa tggtacaccg
    21481 cgcactgcac gcctacgcct ccagcctgaa ccgcccgcgc ctgattatgt tggtcagctt
    21541 tgcggcgttt gtgttgaacg tgccgctgaa ctatattttc gtttacggca aattcggtat
    21601 gcccgctttg ggcggcgcag gctgcggact ggcgacgatg gcggtgtttt ggttcagcgc
    21661 gctggcattg tggatttata tcgccaagga aaatttcttc cgcccattcg gactgacggc
    21721 gaaattcggc aaaccggatt gggcggtgtt caaacagatt tggaaaatcg gcgcacccat
    21781 cgggctgtct tattttttgg aagccagcgc gttttcgttt atcgtgtttt tgattgcgcc
    21841 tttcggcgag gattatgtgg cggcgcagca ggtcggcatc agtttgtcgg ggattctcta
    21901 tatgattccg caaagcgtcg gctcggcggg gacggtgcgc atcggctttt cgcttgggcg
    21961 gcgcgaattt tcgcgggcgc gttatatttc gggcgtgtca ctggtgttag gatggatgct
    22021 cgccgtgatt accgtgcttt ccttggtatt attccgttcg ccgctggtaa gtatgtacaa
    22081 caatgatccg gcggttttaa gcatcgccgc caccgtctta ctgttcgccg gcttgttcca
    22141 accggcagac ttcacccaat gtatcgcctc ctacgccttg cgcggctaca aagttacaaa
    22201 ggtgccgatg ttcatccacg ccgccgcctt ttggggctgc ggtctgctgc cgggctacct
    22261 gctcgcctac cgtttcgata tgggcattta cggcttctgg acggcattga ttgcctcgct
    22321 caccatcgcc gccatcgcct tggtgtggtg cttggaattg tgcagtaggg agatggtcag
    22381 atcgcataag gccgtctgaa aactccatgg gtaggatgaa gtctgctaat tacaatatta
    22441 atagatgatg tggcagggtt gagctaggag aatttagagt ttttgaaggg agagagtttg
    22501 gtgttttagc ttaatctcta tttctaactt aagataagga aaccgttatg tttataaaaa
    22561 gtatttgttt atctaatttt aaagggttta tcggtgataa ccatcagatt aaatttaaaa
    22621 ttcctgatgg agcaactgct ggtagtggtt tgaacatttt tatcggagaa aataactctg
    22681 ggaaatcgtc tatttttgaa gctataaatt tcttaaggaa cggtatcaaa gaagatgaag
    22741 cttatagaat aaaaagtaaa ctggcagatg atactcagcc aaatgacgca tgcgttgagc
    22801 tggctttttg tgggaatatt caaaatgcaa taacttattt tgttcaaagg aataaacaag
    22861 cagcttttaa taatgcaatt aataatgagc agaatttaaa agctaagaga aatacagttt
    22921 cttgtaagaa attggatttg tgggataacg aatctcagga ctattcaaat ccaagtggga
    22981 tcgatgctcc ttttaaggct ctttttgaaa ctaattttat ttgggcagat acaaatccta
    23041 atgatgaagc ggctttcggg tctacaactc tttgcggatt gttactgaaa gaaattgctc
    23101 aggcgcatat caattcggct gaatatcaaa catttcgtga tagttttaat caggtattta
    23161 attcaccagc ttctaatttg cgtcaacaaa ttgctactat cgaacagaaa gttcaacgta
    23221 tttttactga acaattcgga caagctgata ttcattttag atttgatgaa ctaaagatag
    23281 attcgttttt taaaaatgcc tctatcttta ttgacgatgg tgtggatatt ccaatgtccg
    23341 aaaaaggtaa tggtatgcag cgttctgttg cattagcctt gcttcaagtc tatgcagaag
    23401 agttagcgca tgatgaagag caaggtcaga ctaagccgtt ttatttgttt attgatgagc
    23461 ctgagctttg tttacatcca aaagggcaaa ctaaattact tgaggcgttg ttagaaattt
    23521 caagaacgaa acaagtattt ttgactacgc attccccata ttttttggtt acacctcatt
    23581 taagtaatgt tggtctgttt atttttagaa aggacggaat tagcaatatt gttgaagacg
    23641 cttcattgga gccaatgttt ccttggagcc caacttgggg agagattaat ttcaaggcgt
    23701 ataaattacc taccgtggag ttacataatg aattatatgg gaaattacaa catgattgtg
    23761 ggcaatttcg agaaaaagac tttgaacaat ggttacagag taggaatatt cagttcacta
    23821 aacaatggat acgagaaaac aatggatccg cacaagctcc atatgacgta accttgcaaa
    23881 catttatccg caaccatatt catcatcctg aaaatcggcg aaatgcactt tatacggaaa
    23941 atgaacttag acaatctatt gaggaaatga ttagattgtt atagttgaag gagtaaaaag
    24001 tgcggtcaat tctctcaatg ttttcaaaac gtcgtctgaa gccacacttt ctagcccaaa
    24061 gaagaaaatt cagttattca aaatttttgg gtaaccgttg atgatggtaa aaactaaaaa
    24121 actttgatcg cctgctgaaa taaggacaat ttgaggataa acagcaataa acctgtttaa
    24181 tctttgtttt gataaaaaca tatggagctg tcctagataa ctaggataaa ctcgatttta
    24241 ctaattgttt taaaatggaa caagaacttt tatctcactg ttgttaaaac gccgttcgta
    24301 cccctttaaa tacagctcaa aatgcgcttt gggaatgccg ttaaacttgc gtaaatgacg
    24361 ttttgcccgg ttccaaaagt tctcaattcc attaatatgg ttttgtcgtt cagcaaaata
//
LOCUS       DAWFHY010000023        23556 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.023, whole genome shotgun sequence.
ACCESSION   DAWFHY010000023 DAWFHY010000000
VERSION     DAWFHY010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 23556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.023"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(166..2379)
                     /locus_tag="WGQ68_000829"
     CDS             complement(166..2379)
                     /locus_tag="WGQ68_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0714665.1"
                     /translation="MTAISPIQDTQSATLQELREWFDSYCAALPNNDKKLVLAARSLA
                     EAHYPADAATPYGEPLPDHFLGAAQMVHELDLLPDAVAATLLADIGRYVPDWNLLVSE
                     RCNSTVAELVKGVDEVQKLTHFARVDSLATPEERAQQAETMRKMLLAMVTDIRVVLIK
                     LAMRTRTLQFLSNAPDSPEKRAVAKETLDIFAPLANRLGVWQLKWQLEDLGFRHQEPE
                     KYREIALLLDEKRTERLEYIENFLNILRGELKKYNVHFEVAGRPKHIYSIYKKMVKKK
                     LSFDGLFDIRAVRILVDTVPECYTTLGIVHSLWQPIPGEFDDYIANPKGNGYKSLHTV
                     IVGPEDKGVEVQIRTFDMHQFNEFGVAAHWRYKEGGKGDSAYEQKIAWLRQLLDWREN
                     MAESGKEDLAAAFKTELFNDTIYVLTPHGKVLSLPTGATPIDFAYALHSSIGDRCRGA
                     KVEGQIVPLSTPLENGQRVEIITAKEGHPSVNWLYEGWVKSGKAISKIRAYIRQQNAD
                     TVREEGRVQIDKQLAKLTPKPNLQELAENLGFKKLDDLYTAVGQGEISNRAIQKACGT
                     LNEPPPVPVSETTIVKQSKIKKGGKNGVLIDGEDGLMTTLAKCCKPAPPDDIVGFVTR
                     DRGISVHRKTCPSFRHLAEHAPEKVLDASWAALQEGQVFAVDIEIRAQDRSGLLRDVS
                     DALARHKLNVTAVQTQSRDLEASMRFTLEVKQVNDLPRVLASLGDVKGVLSVTRL"
     gene            2708..3349
                     /locus_tag="WGQ68_000830"
     CDS             2708..3349
                     /locus_tag="WGQ68_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrxB family glutaredoxin"
                     /protein_id="HEZ0714666.1"
                     /translation="MKLYIYDHCPFCVRARMAAGLFGADVEEAVLANDDEATPIGMIG
                     AKQVPVLQKEDGSFMGESLDIVRHFDREDRLKDEVRPEIRAWLDKVGGYNDKLVQPRV
                     IKIGLPEFATPEAVKYFTDKKEKSIGSFSANLNKTAQYLERINADLQELENLMDGASD
                     GINGGIGMEDILVFPVLRNLTVVRGIEWPQKVMGYLMTMSEKSGVPLYFDRAL"
     gene            3686..4180
                     /locus_tag="WGQ68_000831"
     CDS             3686..4180
                     /locus_tag="WGQ68_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714667.1"
                     /translation="MDAFDFFMQGNFKKIATEQGLDRKYFIFQYIVCNLSAISSFCLM
                     KIIHTYTILYILIAVIGIINGFLLRSKVLSIVKNKQKFLSDMFPLEMPFFFVEYKIPI
                     LARFLNRGMKWLYLPSFFCLVAICFSIYILNEEGGVDALFCLLLVWTLLSSKHLLRFF
                     IQRL"
     gene            4326..5126
                     /locus_tag="WGQ68_000832"
     CDS             4326..5126
                     /locus_tag="WGQ68_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEZ0714668.1"
                     /translation="MKKTIFNITKMDCPSEEQLIRMRLKDVSDIYELQFDIAGRCLTV
                     YHDNQDTTILQVLEPLNFDSHIISTEVIVDKIVFNKPDEHLEKRLLYQVLMINFVFFI
                     IECSVGIFANSMGLIADSLDMLADSFVYILALSAIGMTLAYKKRVAFLAGITQIILAL
                     FGVIEVIRRFIGAEQLPNYQLMIGTAFLALIANWLCLYLLSKNQNKEIHIKASMIFTS
                     NDIIINIGVIAAGALTLLTHSSYPDLIIGMIVFVIVLFGARNILKLAK"
     gene            complement(5578..6216)
                     /locus_tag="WGQ68_000833"
     CDS             complement(5578..6216)
                     /locus_tag="WGQ68_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEZ0714669.1"
                     /translation="MFQNFDLGVFLLAVLPVLLAITVREVARGYTARYWGDNTAEQYG
                     RLTLNPLPHIDLVGTIIVPLLTLMFTPFLFGWARPIPIDSRNFRNPRLAWRCVAASGP
                     LSNLAMAVLWGVVLVLTPYVGGAYQMPLAQMANYGILINAILFALNIIPILPWDGGIF
                     IDTFLSAKYSQAFRKIEPYGTWIILLLMLTGVLGAFIAPIVRLVIAFVQMFV"
     gene            6471..7313
                     /locus_tag="WGQ68_000834"
     CDS             6471..7313
                     /locus_tag="WGQ68_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEZ0714670.1"
                     /translation="MDKERILTPAVVFSVALLHLAMVALLWQAHKLPVIESGNVIEFV
                     DLGDFGGGDGAPEGAGAPAAPEPQPEPEPPKPVEPPKPVLKPVVTKKADADIQQPKEE
                     PKPEEKPKPEEKPKPEPKPEAKPVPKPAEKPVEKPSEKPAEHPSNASAKADSEQGNGE
                     DKGTGTKGDGTGRGEGSGKGSGGVKGEHGEGAGSSKGNPLRANGSIPRPAYPTLSMEN
                     DEQGTVVLSVLVSPGGHVESVKIVKSSGFSRLDNAARKAAQNGHFQANAWTEFKVPVK
                     FELN"
     gene            7379..8041
                     /gene="exbB"
                     /locus_tag="WGQ68_000835"
     CDS             7379..8041
                     /gene="exbB"
                     /locus_tag="WGQ68_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-system energizer ExbB"
                     /protein_id="HEZ0714671.1"
                     /translation="MNLKLVFESGDPVLIGVFVLMLLMSIVTWCLVVLRCIKLYRARK
                     GNAAVKRHMRDTLSLNDAVEKVRAVDAPLSKLAQEALQSYRNYRRNEASELAQALPLN
                     EYLVIQIRNSMAQIMRRFDYGMTALASIGATAPFIGLFGTVWGIYHALINIGQSGQMS
                     IAAVAGPIGEALVATAAGLFVAIPAVLAYNFLNRGTKILTQDLDAMAHDLHVRLLNQK
                     DS"
     gene            8044..8478
                     /locus_tag="WGQ68_000836"
     CDS             8044..8478
                     /locus_tag="WGQ68_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEZ0714672.1"
                     /translation="MAFGSMNSGDDSPMSDINVTPLVDVMLVLLIVFMITMPVLTHSI
                     PLELPTASEQTNKQDKQPKDPLRLTIDANGGYYVGGDSASKVEIGEVESRLKAAKEQN
                     ENVIVAIAADKAVEYDYVNKALEAARQAGITKIGFVTETKAQ"
     gene            complement(9165..9593)
                     /locus_tag="WGQ68_000837"
     CDS             complement(9165..9593)
                     /locus_tag="WGQ68_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01244 family phosphatase"
                     /protein_id="HEZ0714673.1"
                     /translation="MAILKLDEHLYISPQLTKADAEQIAQLGIKTVICNRPDREEESQ
                     PDFAQIKQWLEQAGVTGFHHQPVTARDIQKHDVETFRQLIGQAEYPVLGYCRTGTRCS
                     LLWGFRRAAEGMPVDEIIRRAQAAGVNLENFRERLDNARV"
     gene            complement(9658..11406)
                     /locus_tag="WGQ68_000838"
     CDS             complement(9658..11406)
                     /locus_tag="WGQ68_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HEZ0714674.1"
                     /translation="MIHYKITSSPLDHEWHILLTFTQDDDLPIEISLPNWVPGSYLIR
                     DFSRHITSIHASCNGTSMPLEQIAKNRWHAAAVRGEWQIRYTVYAFDLSVRGSFLTTE
                     RGFFDGSCLFLKVEGTETLPHRLELTGIPSEWRIATTLPETGRFVFQAASYAELIDRP
                     VEMGLIEFLDFEAAGIPHTIALSGIYPDFDRNRLVSDIKKICETELAVFSSPAPFQKY
                     LFLLHVGDHIYGGLEHADSTALLADRHSLPPYGMTDADDTYTTLLGLFSHEYFHAWNV
                     KSIKPAAFVPYDLDKENYTEQLWAFEGITSYYDDLFLARSRTISPESYLNLLAQGITR
                     VQQTRGRLRQTLAESSFTAWNKFYKPDENSPNAIVSYYQKGALAALCLDLIIRNRSNG
                     RHSLDTLMDKLYREWRDTHSGIPEKHWQIRCQEITGLDLEDFFQKTLYSTEDLPLAEC
                     LATAGVGLTFLPLPRQHGGGYAEHICPVPSAGDFGARFKQNTDHIVLTHVFNGGSAES
                     AALCPQDKIIALDGYACTDFTAQWARYHVNAKINIHFFRAGILRQTVLTVQAAAADTA
                     ILHITDRNLLDNWLFG"
     gene            11733..13166
                     /gene="ccoN"
                     /locus_tag="WGQ68_000839"
     CDS             11733..13166
                     /gene="ccoN"
                     /locus_tag="WGQ68_000839"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HEZ0714675.1"
                     /translation="MDTQTYNYKVVRQFAIMTVVWGIVGMLVGVIVAAQLFAPALDLS
                     NIGPWFHFGRLRPLHTNAVIFAFGGCGLIGTSYYVVQRTCNTRLFGGWLPAFTFWGWQ
                     AVIVAAVVSFPMGWTQGKEYAELEWPIDILITLVWVAYAIVFFGTIAKRKIKHIYVAN
                     WFYGGFILAVALLHIVNNISIPAGLMKSYPVYSGAIDAMVQWWYGHNAVGFFLTAGFL
                     GMMYYFVPKQAARPVYSYRLSVVHFWALIFTYMWAGPHHLHYTALPDWTQSLGMVLSL
                     ILFAPSWGGMINGIMTLSGAWDKLRTDPILKFLIVSLSFYGMSTFEGPMMSIKTVNAL
                     SHYTDWTVAHVHAGALGWVGFVTIGSVYYMIPRLFGKEQMHSTKLVEAHFWIATIGVV
                     LYIAAMWIAGVMQGLMWSSLNDDGTLTYSFVESVKRTMPYYVIRFAGGLLYLSGMCIM
                     AYNVYRTAIGGKAVDAEIPAVSQTQHH"
     gene            13193..13804
                     /gene="ccoO"
                     /locus_tag="WGQ68_000840"
     CDS             13193..13804
                     /gene="ccoO"
                     /locus_tag="WGQ68_000840"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HEZ0714676.1"
                     /translation="MKLQQLAEEKIGVLIVFTLLVVSVGLLIEVVPLAFTKAATQPAP
                     GVKPYNALQVAGRDIYIREGCYNCHSQMIRPFRAETERYGHYSVAGESVYDHPFQWGS
                     KRTGPDLARVGGRYSDEWHRIHLLNPRDVVPESNMPAFPWLARNKVDVDATVANMKAL
                     RKVGTPYSDEEIAKAPEALANKSELDAVVAYLQGLGLALKNVR"
     gene            13809..13979
                     /locus_tag="WGQ68_000841"
     CDS             13809..13979
                     /locus_tag="WGQ68_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HEZ0714677.1"
                     /translation="MDINGIRALFTVWIFICFLLVLYIVFNRRNKKNYDNAANSIFDE
                     NQDAQDKKSENR"
     gene            14003..15100
                     /gene="ccoP"
                     /locus_tag="WGQ68_000842"
     CDS             14003..15100
                     /gene="ccoP"
                     /locus_tag="WGQ68_000842"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HEZ0714678.1"
                     /translation="MNTTSQFTSNFWNIYIAVIVLLSFIALAWLLLSQNVVKRPKKGE
                     EVQTTGHEWDGIAEYDNPLPRWWFWLCVLTWLFGIGYLVMYPGVGDYKGLLKWTSHNQ
                     YEKEVKKADEQYGKLYAKFADMPIEKVAKDPQAKQIAQNLFNTYCIQCHGSDAKGSKG
                     FPNLTDSDWLWGGDPDKIHETIEKGRVATMPAWGPALGEEGVKDVAHYVMSLSKPEGQ
                     YDEERAARGQALFSGPPANCFTCHGDKGQGIQGLGPNLTDDVWLWGGTQKSIIETITN
                     GRSSQMPAWGHFLDKDKLHIMTAYVWGLSDKDGKAPVKKAEPAPTPAPAAEPAASAPA
                     EAAQAASEAKPAAAEPKAEEKAAPAAKADGK"
     gene            15213..15338
                     /locus_tag="WGQ68_000843"
     CDS             15213..15338
                     /locus_tag="WGQ68_000843"
                     /inference="COORDINATES: protein motif:HMM:NF024834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714679.1"
                     /translation="MHKHAIEGFNTMPAKGGRGDLSADEVKAAVDYMVNQSGGKF"
     gene            15540..16949
                     /locus_tag="WGQ68_000844"
     CDS             15540..16949
                     /locus_tag="WGQ68_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEZ0714680.1"
                     /translation="MDTQIKTEAGQQNNRRYLTVWRWHFYAGLLVMPFLTLLAVTGLG
                     MLLFANITGKEGERIHVVPQATVQPLSVQAEAARSAVNPETSSVVQYIAPRAEDMVAV
                     FRVNNEGKATMVAVDPYTAKVVSTMPRNQGWYHTMDEIHSDMMLGSTGDYLLETAASL
                     TIIMIISGLYLWWVKRRGIKAMLLPSKGRARSWWRNLHGTFGTWVSLILLLFCLSGIA
                     WAGIWGGKFVQAWSQFPAGKWGVEPNPVSVVPTHGEVLNDGKVKEVPWVLELTPMPVS
                     GTTVGKDGINPDEPMTLETVDRFAREIGFKGRYQLNLPKGEDGVWTLSQDSMSYDMIS
                     PFADRTVHIDQYSGKILADIRFDDYNPFGKFMAASIALHMGTLGWWSVLANVLFCLAV
                     IFIGISGCVMWWKRRPTGAVGIVPPAQKVKLPVWWMMALPLLAIALLFPTALLAIAVI
                     WLLDTLLLSRIPVLRRWFK"
     gene            17050..20259
                     /gene="recC"
                     /locus_tag="WGQ68_000845"
     CDS             17050..20259
                     /gene="recC"
                     /locus_tag="WGQ68_000845"
                     /EC_number="3.1.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HEZ0714681.1"
                     /translation="MFYLYQSNRLETLAALFARIQKVKPLKSALQPEQIIVQSQGMRR
                     YLNTCLARDLGVAANLSFSLPAGLTWKLMKKLIPGIPELSPFAPEVMRWRLLDLFRSE
                     AFRNTAEFEDVRNVLQDYLGSGESADYQLAGQLADIFDQYLVYRPQWIDAWQQGRRLG
                     LGDDEIWQSKLWRYLDDGRQSAPHRVALWEKLLAALDKDKLPERYFVFGISTMAPMYL
                     QLLHKLSEHCDVFVFALNPSGMYWGNVIEAAQILKGGGDPDLTQAGHPLLASLGKQGR
                     DFFDFLNEMEIEGETPVFEEGGRDTLLHALQTDIQNLKMPSENVGSVNTGDGSIRIVS
                     AHSPLRELQILKDKLLKILHEHPDWQPHDIAVLTPHIESYTPFIEAVFGQAQPGAQAL
                     PYSVSDVKISRRQPLFHALSCLFDLLESRFEVDKVLVLLETAPVLRRFGLTEDDLPLL
                     HDMVADLNVHWGLDGEMRGGTDQLFTWKQAVERMILGWMLPEGGNPMWQDVSAWYADV
                     NQTALFGRFAALLETLSDIVRIWRQPATIGEWVARCRDLLETLFQAEADDQKSVQNLE
                     NEWVKWQAETELAQFSGQLPQQTVIRHIRRFLDSESEAGFLRGGITFCSMVPMRSLPF
                     KVICLLGLNDGDFPRNTKAAVFDLIAKHPAKGDRARRDDDRYLFLEALISAREILYLS
                     YIGRDIRKDEELAPSSLLGELIDTVAAMAGIGSRQLAQNWIEQHPLQAFSRRYFQEGG
                     RSDGIFGTRTDYATALGQTPEPPQPFFDQPVENAEPVAEIGQDEFIRFWRNPVKVWLQ
                     QQLAWSEPHIGEAWEPAEPFEPQHADQIAETYIEARREGRDFAQTAARIGAESLLPSG
                     ELGRLWQQDFQTAAKQIDTAVLNSPKLPPLPYAIPSDGQILKGSLGNLYRCGQVFYAY
                     GKPNAPQRIAFLLEHLIFCAVMPSEAETRQTFIVQSGETEILAEIAQDRALQLLSEWM
                     AFFNIGQNRPLPFFAKTSLATAEAFAQKQDWEAALKKAQTAYHGSKVSKGQKDYTEVA
                     LVFGNASQNPLEQPLFENLVRLLADTLAAAEKKEEAGEA"
     gene            complement(20327..21895)
                     /gene="mtrF"
                     /locus_tag="WGQ68_000846"
     CDS             complement(20327..21895)
                     /gene="mtrF"
                     /locus_tag="WGQ68_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041421321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family antimetabolite efflux transporter
                     MtrF"
                     /protein_id="HEZ0714682.1"
                     /translation="MNQTDTRRGGRFLRTVEWLGNMLPHPVTLFIIFIVLLLIASAVG
                     AYFGLSVPDPRPVGAKGRADDGLIYVVSLLNADGLIKILTHTVKNFTGFAPLGTVLVS
                     LLGVGIAEKSGLISALMRLLLTKSPRKLTTFMVVFTGILSNTASELGYVVLIPLSAVI
                     FHSLGRHPLAGLAAAFAGVSGGYSANLFLGTIDPLLAGITQQAAQIIHPDYVVGPEAN
                     WFFMVASTFVIALIGYFVTEKIVEPQLGPYQSDLSQEEKDIRHSNEITPLEYKGLIWA
                     GVVFVALSALLAWSIVPADGILRHPETGLVSGSPFLKSIVVFIFLLFALPGIVYGRVT
                     RSLRGEQEVVNAMAESMSTLGLYLVIIFFAAQFVAFFNWTNIGQYIAVKGATFLKEVG
                     LGGSVLFIGFILICAFINLMIGSASAQWAVTAPIFVPMLMLAGYAPEVIQAAYRIGDS
                     VTNIITPMMSYFGLIMATVIKYKKDAGVGTLISMMLPYSAFFLIAWIALFCIWVFVLG
                     LPVGPGAPTFYPAP"
     gene            22227..22439
                     /locus_tag="WGQ68_000847"
     CDS             22227..22439
                     /locus_tag="WGQ68_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714683.1"
                     /translation="MKNEIQKIMDKYDPWHEDDFESYEDIARDVSLMTDKTFIEHYLL
                     EVYSEENGHFDQENVHAMIEEIKNAI"
     gene            complement(22718..23350)
                     /gene="mtrR"
                     /locus_tag="WGQ68_000848"
     CDS             complement(22718..23350)
                     /gene="mtrR"
                     /locus_tag="WGQ68_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux system transcriptional
                     repressor MtrR"
                     /protein_id="HEZ0714684.1"
                     /translation="MRKTKTEALKTKEHLMLAALETFYRKGIARTSLNEIAQAAGVTR
                     GALYWHFKNKEDLFDALFQRICDDIENCIAQDAEDAEGGSWAVFRHTLLHFFERLQSN
                     DIYYKFHNILFLKCEHTEQNAAVIAIARKHQAIWREKITAVLTEAVENQDLADDLDKE
                     TAVIFIKSTLDGLIWRWFSSCERFDLGKTAPRIIGIMMDNLESHPCLRRK"
BASE COUNT         5915 a         5424 c         6229 g         5988 t
ORIGIN      
        1 ttaaagcttt gtttcttaag tccgcagagt atgccatggt tagaccttca aagttgagta
       61 ttgtactatt ttgtttttaa ttgactatgc aacaaaaaat agcaaacccc gtcaatcaaa
      121 atgccgtctg aagcgttata cggctttcag acggcatttt tgtatttaaa gccgggtaac
      181 gctcaatacg cctttgacgt cgccgaggct ggcgaggacg cgcgggaggt cgttgacctg
      241 tttgacttcg agcgtgaacc gcatactggc ttccaagtcg cgggactggg tttgcacggc
      301 ggtaacgttg agtttgtggc gggcgagcgc gtcggatacg tcgcgcaaaa gcccggagcg
      361 gtcttgggcg cggatttcga tatcgacggc gaacacttgt ccttcctgca acgccgccca
      421 acttgcgtcc agtacttttt cgggcgcgtg ttcggcgagg tgtcggaaag aggggcaggt
      481 tttgcggtgt accgaaatgc cgcgatcgcg ggtaacgaag ccgacaatgt cgtcgggcgg
      541 cgcgggtttg cagcatttgg caagcgtggt catcaaaccg tcttcgccgt cgatgagcac
      601 gccgtttttg ccgccttttt tgattttgga ctgtttgacg atggtggttt cgctgacggg
      661 tacgggcgga ggttcgttca gcgtgccgca ggctttttgg atggcgcggt tggaaatttc
      721 gccttgtccg acggcagtgt agaggtcgtc gagttttttg aagccgaggt tttcggcaag
      781 ctcttgcagg ttgggcttgg gcgtgagttt ggcaagctgc ttgtcgattt ggacgcggcc
      841 ttcttcgcgc acggtgtcgg cgttttgctg gcggatgtag gcgcggattt tactgattgc
      901 cttgccggat ttgacccagc cttcgtacag ccagttgacg gaaggatgcc cttctttggc
      961 ggtaatgatt tcgacgcgct gtccgttttc aagcggggtg gacagcggca cgatttgccc
     1021 ttcgactttc gcgccccggc agcggtcgcc gatgctgctg tgcagggcgt aggcgaagtc
     1081 gatgggtgtc gcgcccgtag gcagggaaag gactttgccg tgcggggtta aaacgtaaat
     1141 cgtgtcgttg aacagctcgg ttttgaaggc ggcggcgagg tcttccttgc cgctttccgc
     1201 catattttcg cgccagtcca aaagttggcg caaccaggcg attttttgct cgtaggcgga
     1261 atcgcctttg ccgccttctt tgtaacgcca gtgcgcggcg acaccgaatt cgttgaattg
     1321 gtgcatatcg aaggtgcgga tttgcacttc cacacctttg tcttcgggac cgacgatgac
     1381 ggtgtgcaaa cttttatagc cgttgccttt cgggttggcg atgtagtcgt cgaactcgcc
     1441 ggggataggc tgccaaaggc tgtggacaat gcccagcgtg gtgtaacact cggggacggt
     1501 atcaaccaga atccgcacgg cgcggatgtc aaagaggccg tcgaagctga gttttttctt
     1561 caccattttt ttgtaaatgg agtagatgtg tttcgggcgg ccggcgactt cgaaatggac
     1621 attgtatttc ttgagttcac cgcgcaggat gttgaggaag ttttcgatgt attcgaggcg
     1681 ttcggtgcgt ttttcgtcca aaagcagcgc gatttcgcgg tatttttcgg gctcttgatg
     1741 gcggaagccc aaatcttcga gctgccattt gagctgccac acgcccaaac ggttggcgag
     1801 cggggcgaag atgtcgaggg tttctttggc gacggcgcgt ttttcggggc tgtcgggggc
     1861 gttgcttaaa aattgcaggg tgcgcgtacg catcgccagt ttgattaaca cgacgcggat
     1921 gtcggtaacc atcgccagca gcattttccg catggtttcc gcttgctggg cgcgttcttc
     1981 tggcgtggcg aggctgtcca cccgggcgaa gtgggtgagt ttctgcactt cgtccacacc
     2041 tttgaccagc tcggcgacgg tactgttgca gcgttcggaa accaataggt tccaatcggg
     2101 gacgtagcgt ccgatgtcgg caagcagggt ggcggcgacg gcatcgggga gcaggtcgag
     2161 ttcatgaacc atttgcgccg cgccgaggaa gtggtcgggc agcggctcgc catacggcgt
     2221 ggcggcatcg gcggggtaat gtgcttccgc cagcgaacgg gcggctaaga caagtttttt
     2281 atcgttgttc ggcagcgcgg cgcagtagct gtcgaaccat tcgcgcaatt cttgcagagt
     2341 cgcgctttgc gtgtcttgaa tcgggctgat ggcggtcatg atggggtctt ccgttccgat
     2401 aaaaacgagt ttattgtaac agcttgagac ctttgcaata acataggtta ctaaaatttt
     2461 atgctcaatc tcattttcaa aatgcaaaac ttttctgatt tttcctactt tttgctcaat
     2521 attaggaagg ttttaggcaa ttgaaaattt tttggcgcat ttttatgcgt caaatttcgt
     2581 taacagacta tttttgcaaa ggtctcagct tatttattta cggtgcatag gcgggaagat
     2641 aaacttgttt cgggtggcgc ggggtgttaa ggtagccggt accgacaggt tgacgaggaa
     2701 tattttgatg aaactgtata tttacgacca ttgcccgttt tgcgtgcgtg cgcggatggc
     2761 tgccgggctg tttggcgcgg acgttgaaga agccgtgctg gcaaacgacg acgaggcaac
     2821 gccgatcggt atgatcggtg cgaaacaagt gcccgtcctg caaaaagaag acggttcgtt
     2881 tatgggcgag agtttggaca ttgtgcggca tttcgaccgc gaagaccgtc tgaaagacga
     2941 agtccgcccc gagatacggg cgtggctgga caaggtgggc ggatacaacg acaaactggt
     3001 gcagccgcgc gtgatcaaaa tcgggctgcc tgaatttgca acgccggaag cggtgaaata
     3061 ttttacagac aaaaaagaga aaagcatcgg cagcttttcc gccaatttga ataaaaccgc
     3121 ccaatacctc gaacggataa acgcagattt gcaagagttg gaaaatttga tggacggtgc
     3181 ttcagacggc atcaacggcg gaatcggtat ggaggatatt ttggttttcc ccgttttgag
     3241 gaatctgacc gtggtgcgcg gcatcgaatg gccccaaaag gtgatgggtt atttgatgac
     3301 gatgagtgaa aaatcgggcg tgccgcttta ttttgacagg gcattgtaaa agtggctgtt
     3361 attaactgac agatgaagga ggttctaccc tatttgaggt aaactctatt taccattggc
     3421 cgaaatacca gtgcttatct ccaagacgac cttcccaatc ccttttgcct cttaaaaaga
     3481 agtgactagt gtgtaaagtt atagttcctt ttatagcttt ccaataaaat taaagtattt
     3541 aaaagcttgt ttagttctaa tttgttaatt tctgtatcta aataatgttc agaatagtat
     3601 tattttttaa ataaaccatg aatagtactt cacattgttt gtcgtattat ttagctttta
     3661 aatttctaat gtgaaagata gtttaatgga cgcctttgat ttttttatgc aaggcaattt
     3721 taaaaaaatt gcaactgaac aaggtttaga ccgtaaatat tttatattcc aatatatcgt
     3781 atgtaattta tctgccattt cttctttttg tctaatgaag attattcata cttatactat
     3841 tctctatata ttaatagctg tcatcggtat tattaatggc tttctcttga gaagcaaggt
     3901 tctatctata gtaaaaaata aacaaaagtt tttatctgac atgtttccct tggaaatgcc
     3961 tttctttttt gtagaataca aaatacctat cttggctaga ttcttgaata ggggaatgaa
     4021 atggctttat ctcccaagtt ttttctgttt agttgcgatt tgtttctcaa tatatatcct
     4081 taatgaagaa gggggggtgg atgccttatt ttgcctgcta ttggtttgga ctttgttatc
     4141 cagtaagcac ttattacgtt tttttatcca gagattataa acatatctaa actattatgc
     4201 gtggcggtac ttccaaaggt aaattttaac ctaatcaatt tactcaaacc atccaaaaat
     4261 taccaagcag caatataaac acagatttat ttgagccaac ctgatcaaaa caaggaacta
     4321 aatagatgaa aaagaccatt tttaacatta ccaaaatgga ctgcccgtcc gaagaacagc
     4381 tcattcgcat gcgtttaaaa gatgtgtctg atatttatga attacagttt gatattgctg
     4441 ggcggtgctt aaccgtctat cacgataatc aagatacaac gattttacaa gtacttgaac
     4501 cgcttaattt tgatagtcat atcatttcca ccgaagtgat tgttgataag atagtgttta
     4561 acaagcctga tgagcactta gaaaaaagat tattatatca agtgctcatg attaactttg
     4621 tgttttttat catagagtgt tcggttggta tttttgctaa ttctatgggg ctgattgctg
     4681 atagtctaga tatgctggcg gacagctttg tctatatatt ggcgctatca gccattggta
     4741 tgacgctggc gtataaaaaa cgtgtggcat ttttggcggg gataacgcag ataatattgg
     4801 cgttatttgg ggtgatagaa gtgattaggc gttttatcgg tgctgagcag ctgcccaatt
     4861 atcaactgat gattggcacg gcatttttgg cattgattgc aaactggctg tgcttatatc
     4921 tactgagcaa aaatcaaaat aaagaaatcc atatcaaagc cagcatgatt tttacatcca
     4981 atgacattat catcaatatc ggtgtgatag cggcgggagc attgacgtta ttgactcatt
     5041 ctagctatcc tgatttaatc attggcatga ttgtctttgt cattgtacta tttggtgcca
     5101 gaaatatttt aaaattggca aaataattcc tttcaccagc aaaggagttt ttatgcaaat
     5161 atcggtcaaa gctacctaca tacgtggcag tacttccaaa aacttgattt tttaaaatgt
     5221 ggttaatgcg agtttgttta tctggtattt gattggtcaa gccttcatcc aagcgtttga
     5281 cgatttcacg caaaaatacc cctgatttac acacagggtc taagaatgtc gcctttggat
     5341 ttgaccaaat ctcagacggc aataaatcca gcatttgatt ggcaagtttt ggcgatgtga
     5401 aaacctcatc attagacaga ctggagtttc ttaatcagtg tttcagttaa tgaaaggtat
     5461 tatcatgcaa cctggttcta ataatgatga aaaaaataag attacactac aggaaaacgc
     5521 ggcagggcaa tatgtcctgc cgcgttttct ggagcgttta tgccgtctga aagccagtca
     5581 gacgaacatc tgcacaaacg caatcaccag ccgcacaatc ggtgcaataa acgcacccaa
     5641 aaccccggtc agcatcagca gtaggataat ccacgtccca taaggttcga ttttgcggaa
     5701 cgcttgcgaa tatttcgccg acaggaaggt gtcgatgaaa atgccgccgt cccaaggcag
     5761 gatggggatg atgttgagcg cgaacagaat cgcattgatc agaataccgt agtttgccat
     5821 ttgagccaac ggcatctgat acgccccgcc gacatacgga gtcagcacca aaaccacgcc
     5881 ccacagaaca gccatcgcca gattcgacag cgggccggac gcggcaacgc aacgccaggc
     5941 aaggcgcggg ttgcggaagt tgcgcgaatc gataggaatc ggacgcgccc agccgaacag
     6001 gaagggcgtg aacatcaaag taagcagcgg tacgatgatt gtgccgacca aatcgatatg
     6061 gggtaggggg ttcagtgtca gcctgccgta ttgttcggca gtgttgtctc cccagtagcg
     6121 cgccgtatag ccgcgcgcca cctccctgac ggtaatggcg agcagcacgg gcaggacggc
     6181 aagcagaaac acgcccaaat caaaattttg aaacatggtt ttcctttgaa cagatgtggt
     6241 gtgcagcccg aaacggctga cagacagaag cggcaatata tcatctaacc tgattttatt
     6301 ggaataagaa atcagacgga atcatttttt ctgccggccc gttatctgat gatttgctgt
     6361 aatacagcgt ttaaacacgg tttgcctgtg aacggaacag acattcatat gaccaaaaat
     6421 tgtaaaaata ggttgcaaat aggaattgtt gctattatta tgcccgaatt atggataaag
     6481 aacgaatttt aacccccgca gtcgtgtttt ccgtcgcact gctgcatttg gcaatggtgg
     6541 cattgctctg gcaggcgcac aagctgcccg tgatagagtc aggaaatgtt atcgaatttg
     6601 tcgatttggg tgattttggc ggaggggacg gtgcacccga aggcgcaggt gcgcctgctg
     6661 cgcctgagcc gcaacctgag cctgagccgc ccaaacctgt cgagccgccc aagccggttt
     6721 tgaagccggt ggttacgaaa aaggcggatg cggatattca gcagcctaag gaagagccga
     6781 aacctgaaga aaagccgaaa cccgaagaaa aaccgaaacc agagcctaag ccggaagcga
     6841 agcctgtccc gaaaccggcg gaaaaaccgg tcgagaagcc gtctgaaaaa cctgccgaac
     6901 atcccagcaa tgcttctgcc aaagcagaca gcgagcaggg taatggggaa gataagggaa
     6961 ccggtaccaa aggagacgga acggggcgcg gagaaggcag cggtaaaggt agcggcggtg
     7021 tcaaaggcga acacggggaa ggagccggca gcagcaaagg caatccttta cgcgccaacg
     7081 gcagcattcc gcgcccggct tatcccacgc tttctatgga gaatgacgag cagggtacgg
     7141 ttgttttgag cgttttggtt tctccgggcg gtcatgtcga gtccgttaaa atcgtgaaaa
     7201 gcagtggttt ttctcggttg gacaatgcgg cacgcaaggc ggcgcaaaac gggcattttc
     7261 aagccaatgc ctggacggag tttaaagtcc ccgtcaagtt tgaattgaat taggcggttg
     7321 ccgcagcggt ttgcggcagc ttggatgttt gattggtttt ttcagtgttg gaatcattat
     7381 gaatttgaaa ttagtatttg aatcgggcga tcccgtcctg atcggtgtgt ttgtgttgat
     7441 gctgttgatg agtatcgtaa cgtggtgttt ggttgtcttg cgctgcatca agctgtatcg
     7501 ggcgcgcaaa gggaatgccg ccgtcaaacg gcatatgcgc gatactttgt cgctgaacga
     7561 cgcggtcgaa aaagtgcgcg ccgtcgatgc gcctttgtcc aaactggcgc aagaggcatt
     7621 gcagtcttac cgcaactacc gccgaaacga agcgtccgaa ctggcgcagg ctttgccgtt
     7681 gaacgagtat ttggtcattc aaatccgcaa cagtatggcg cagattatgc gccggtttga
     7741 ttacgggatg accgcgcttg cctccatcgg cgcgaccgcg ccgtttatcg ggctgttcgg
     7801 cacggtttgg gggatttacc acgccctgat caatatcggg caaagcgggc agatgagtat
     7861 tgcggcggtt gccggcccga ttggcgaggc actggtggcg acggcggcgg gtttgttcgt
     7921 ggcgattccg gcggtgttgg catacaactt cctcaatcgc ggcacaaaaa tactgaccca
     7981 ggatttggat gcgatggcgc acgatttgca cgtccgcctg cttaatcaaa aggatagctg
     8041 attatggcat ttggttcgat gaattccggc gacgattctc cgatgtccga catcaacgtt
     8101 acgccgttgg tggacgtgat gctggtgttg ctgattgtgt ttatgattac tatgccggtg
     8161 ctgacgcatt ccatcccttt ggaactgccg accgcgtccg agcagacaaa caagcaggac
     8221 aaacagccta aagaccccct gcgcctgacg attgatgcga acggcggcta ttatgtcggc
     8281 ggggattctg caagcaaagt ggaaatcggg gaagtggaaa gccgtctgaa agccgccaag
     8341 gagcagaatg aaaacgtgat tgtggcgatt gcggcagaca aggcggtgga atacgattat
     8401 gtaaacaaag ctttagaagc cgcccgtcag gcaggaatca ccaaaatcgg ttttgtaacc
     8461 gaaaccaagg cgcaataaag ttcgacgctt gaacgaatgc cgtctgaaag tctttcagac
     8521 ggcatttctt atgtttaatc agctatatat agttgattaa aacaaaaatg agacgacaga
     8581 gcgccgtctc catttctgcg caggcgggaa tacaaacatc tgatttttct tttattttca
     8641 aacattactg aaatgggtaa gtctggagtc ctgcctgcgc gagaataatg aaaatgtgca
     8701 tttctaattt ttatagtgga ttaacaaaaa tcaggacaag gcaacgaagc cgtagacagt
     8761 acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
     8821 agttaaggcg aggcaacgcc gtactggttt ttgttaatcc gctataccca ctgagacctt
     8881 tgcaaaaata gtctgttaac gaaatttgac gcataaaaat gcgccaaaaa attttcaatt
     8941 gcctaaaacc ttcctaatat tgagcaaaaa gtaggaaaaa tcagaaaagt tctgcatttt
     9001 gaaaatgagt ttgagcataa aattttagta acctatgtta ttgcaaaggt ctcccactgt
     9061 aaaaacggag aaggacagag gacggcagta aggtttgccg tcctcttttt caagttgcgg
     9121 tggaatgccg cttgaaggtg tggtttaaac gtttcggctt gtaatcagac gcgggcgttg
     9181 tccagccgct ctctgaagtt ttccaaattt acgcctgccg cttgggcgcg gcggatgatt
     9241 tcgtcaaccg gcataccttc tgccgcccgg cggaagcccc acaggaggga gcagcgcgta
     9301 ccggtccggc aatagccaag gacgggatat tcggcttgtc cgatgagttg gcggaaggtt
     9361 tcgacatcgt gtttttggat gtcgcgtgcg gtaacgggtt ggtgatggaa tccagtaacg
     9421 cctgcttgtt ccagccactg tttgatttgg gcgaagtcgg gttgcgattc ttcttcgcgg
     9481 tcggggcggt tgcagatgac ggttttgatg cccagttgcg cgatttgttc cgcatcggct
     9541 ttggtcagtt gcggagaaat atagaggtgt tcgtcaagtt tcagaatggc cattttgttt
     9601 cctttacttt gcggttgttt ttcagacggc attttgtgtg caatgccgtc tgaaagttta
     9661 accgaacaac cagttgtcca acaggtttcg gtctgtgata tgtaggatgg cagtatccgc
     9721 tgccgctgcc tgaaccgtca agacggtttg acgcaatatg ccggcacgga agaagtggat
     9781 attgattttt gcattgacgt ggtatcgggc ccattgtgcg gtaaagtcgg tgcaggcata
     9841 accgtctaaa gcaatgattt tgtcttgcgg gcacagtgcc gcagattccg cgctgccgcc
     9901 gttgaagaca tgggtcagga cgatgtggtc ggtgttttgt ttgaaacgtg cgccaaaatc
     9961 gcctgccgac gggacggggc agatgtgttc tgcgtatccg ccgccgtgtt gtcggggaag
    10021 cggcaggaag gtcagtccca cgcctgcggt tgccaggcat tcggcaagcg gcaaatcttc
    10081 ggtactgtat aacgtttttt ggaaaaaatc ttccaaatcc aagccggtaa tttcctgaca
    10141 gcggatttgc cagtgttttt ccggaatacc cgagtgtgtg tccctccact cccgatagag
    10201 tttgtccatt aacgtatcga gagaatgtct gccgttgctt cggttgcgta ttatcagatc
    10261 aaggcacaat gcggcaagcg cgcctttctg gtagtagctg acgatggcgt tggggctgtt
    10321 ttcatccggt ttgtaaaatt tgttccacgc ggtaaaactc gattccgcca aggtctgcct
    10381 caaacggccg cgggtttgtt gtacgcgcgt aatgccttgt gccagcaggt ttaaataaga
    10441 ttcgggcgag atggtgcggc tgcgtgccaa aaacaaatcg tcgtaatagg atgtaatgcc
    10501 ttcgaatgcc catagttgtt cggtatagtt ttctttgtcg aggtcataag ggacgaacgc
    10561 ggcaggtttg atggatttga cgttccacgc gtgaaaatat tcgtgggaga aaagtccgag
    10621 caatgtggtg taggtatcgt cggcatcggt cataccgtac ggcggaaggc tgtggcggtc
    10681 ggcgagcagg gcggtgctgt cggcgtgttc caaaccgccg taaatatggt cgccgacgtg
    10741 gagcaggaac aaatattttt gaaacggggc aggggaggaa aacaccgcca gttctgtttc
    10801 gcagattttt ttgatatccg aaaccagcct gttgcggtcg aaatcgggat atatgccgct
    10861 taaggcaatt gtgtgcggaa tgcctgccgc ctcaaaatct aaaaattcaa tcaagcccat
    10921 ctcgacaggt cggtcaatca attcggcata agatgccgcc tgaaagacaa acctccctgt
    10981 ttccggcagc gttgtggcaa tacgccattc ggacggaata cccgtcaatt ccaagcggtg
    11041 cggcagcgtt tccgttcctt cgactttcaa aaacaggcac gatccgtcaa aaaaaccgcg
    11101 ttctgtcgtc aggaaagaac ctcgaaccga caaatcgaat gcatatacgg tgtagcggat
    11161 ttgccactcg ccgcgtacgg cggcagcgtg ccagcggttt tttgcaattt gttcgagcgg
    11221 catggacgtg ccgttacagg atgcatggat agaagtgatg tggcgggaaa aatcccgaat
    11281 cagatagctg cccggaaccc agtttggcag gcttatttct ataggaagat catcatcttg
    11341 tgtgaatgtc agcaggatgt gccactcgtg gtcgagaggg gacgaggtta ttttataatg
    11401 aatcataact atcaaatata tcgttaaacg ggatggatta aataccttta aaatgcagct
    11461 taacctacat caataaagtg ccacaaaaag ggaaaattgg aaacaaaagc aatggcggtc
    11521 gggttgtaat ttattataac aatatgattt atatgtaata aaaaacagat taagcaaaga
    11581 gctcgccggc ggcgcgaatg taattccgct tggcgtaata aggcagatat gttaaattta
    11641 gaaaaaatta cgcttgcccg gattgcacct taaacaggct gcggaagcgg caggataaaa
    11701 atctttattc ccataaattc atggagactc tcatggacac acaaacttac aactacaaag
    11761 tggtgcgcca gttcgccatc atgactgtag tttgggggat tgtgggtatg ttggtcggcg
    11821 ttatcgtcgc cgcccagctt tttgctcctg ccctcgactt gtctaatatc ggaccttggt
    11881 tccacttcgg ccgcctgcgt ccgctgcaca ccaatgcggt tattttcgca tttggcggtt
    11941 gcggcctgat cggcacatca tactacgttg tccaacgtac ttgtaatacc cgtcttttcg
    12001 gcggttggct gccggcattt accttttggg gctggcaggc ggtaatcgtt gccgccgtcg
    12061 tcagcttccc tatgggttgg acccaaggta aggaatatgc cgaactggaa tggccgatcg
    12121 atattctgat tactttggtt tgggtggctt acgccatcgt attcttcggt acgattgcca
    12181 aacgtaagat taaacatatt tacgttgcca actggttcta cggcggcttt attttggcgg
    12241 tcgcactttt acacatcgtc aacaatatca gcatcccggc agggttgatg aagtcatacc
    12301 ccgtctattc cggtgcgatt gatgctatgg ttcaatggtg gtacgggcat aacgccgtgg
    12361 gcttcttcct gactgccggc ttcttgggta tgatgtacta tttcgtaccc aaacaagcag
    12421 cccgccccgt ttactcctac cgcctgtccg tcgttcactt ctgggcgttg attttcacct
    12481 atatgtgggc gggtccgcac catcttcact acactgcgct gcctgactgg acgcaatctt
    12541 tgggtatggt tctgtctttg attctgttcg caccctcttg gggcggtatg attaacggca
    12601 tcatgacctt gtccggcgcg tgggacaaac tgcgtacaga cccgattctt aaattcctga
    12661 ttgtatcctt gtccttctac ggtatgtcta cctttgaagg cccgatgatg tcgattaaaa
    12721 cggtcaatgc attgagccac tatacggact ggaccgtcgc gcacgttcat gcgggtgcgt
    12781 tgggctgggt aggctttgta accatcggtt ccgtctatta catgattccc cgtctgttcg
    12841 gcaaagaaca gatgcacagc accaagctgg tagaagcaca tttttggatt gcgaccatcg
    12901 gcgtggttct gtatatcgct gccatgtgga ttgccggtgt gatgcagggt ttgatgtgga
    12961 gttctttgaa cgatgacggt acgctgacct actcgtttgt cgaatccgta aaacgcacca
    13021 tgccttacta cgtgatccgt tttgcaggtg gtttattgta tctgagtggt atgtgcatta
    13081 tggcgtacaa cgtgtaccgc acagccatcg gtggtaaagc agtcgatgcc gaaatccctg
    13141 cggtttccca aacacagcac cactaaaata ctaagaaaga taggctacca aaatgaaatt
    13201 acaacaattg gctgaagaaa aaatcggcgt tctgattgtg ttcacgctgc ttgtagtcag
    13261 tgtcggtctg ttgattgaag ttgtgccctt ggcctttacc aaggcggcaa cacagccggc
    13321 gccgggcgtg aagccttaca atgccctgca ggttgccgga cgcgatattt acatccgtga
    13381 gggctgttac aactgccact cgcaaatgat tcgtccgttc cgtgcggaaa ccgagcgtta
    13441 cggtcattac tctgttgccg gagagtcggt ttacgaccat ccgttccaat ggggttccaa
    13501 acgtaccggt cctgatttgg cacgtgtggg cggtcgctat tccgacgaat ggcaccgtat
    13561 ccacctgctg aatccccgtg atgtcgtgcc tgagtccaat atgccggcat tcccgtggct
    13621 tgcacgcaat aaagtcgatg tcgatgcaac cgttgccaac atgaaggctt tgcgtaaagt
    13681 aggtactcct tacagtgatg aggaaattgc gaaagcacct gaggctttgg caaacaaatc
    13741 cgagctggat gctgtagtcg cctatctgca aggattgggt ctggctttga aaaacgtaag
    13801 gtaacatcat ggatattaac ggtattcgtg ctctcttcac ggtatggatc tttatctgtt
    13861 tcctgttggt actctatatc gtcttcaaca ggcggaataa gaaaaactac gataatgccg
    13921 caaacagcat ttttgatgaa aaccaagatg cgcaagataa gaaaagcgaa aaccgttaat
    13981 attgtgataa cggagcaaaa caatgaacac aacatcccaa tttaccagta atttctggaa
    14041 tatatatatt gcagttattg tcttactgag ctttatcgct ttggcttggc tgctgctgtc
    14101 tcaaaatgtt gtcaaacgtc cgaagaaggg cgaagaagta caaactacgg gtcatgagtg
    14161 ggacggcatt gccgaatacg acaatccgct gccccgctgg tggttttggc tgtgtgtttt
    14221 gacgtggctg ttcggtatcg gttatttggt tatgtatccg ggtgtcggcg actacaaagg
    14281 tctgctgaaa tggaccagcc ataaccaata tgaaaaagag gtcaaaaaag ccgatgagca
    14341 atacggcaaa ctgtatgcca agtttgcgga tatgccgatt gaaaaagtgg caaaagaccc
    14401 tcaagccaag caaatcgccc aaaacctgtt taacacttat tgtatccagt gccacggctc
    14461 tgatgctaaa ggctctaaag gttttccgaa tctgaccgat agcgattggt tgtggggcgg
    14521 tgatcccgat aaaatccacg agaccatcga aaaaggccgt gttgcgacta tgcctgcctg
    14581 gggtcctgct ttgggcgaag aaggcgtgaa agacgttgcc cattatgtga tgtccctttc
    14641 aaaacccgaa ggtcagtatg atgaggaacg tgccgcgcgc ggacaagcct tgttcagcgg
    14701 tccgcctgcc aactgtttca cttgccacgg cgataaggga caaggtatcc aaggtttggg
    14761 tccgaacctg actgatgacg tgtggttgtg gggcggtacg caaaaatcca ttatcgaaac
    14821 cattaccaac ggtcgcagca gccaaatgcc cgcttgggga catttcttgg ataaagacaa
    14881 actgcatatt atgactgctt atgtatgggg tctttccgat aaagacggta aagctccggt
    14941 gaaaaaagcc gagcctgcac cgactcccgc accggcggca gaacccgcag cctctgctcc
    15001 tgcagaagca gcacaagccg cgtccgaagc caaacctgcc gcagcagaac ctaaagccga
    15061 ggaaaaagcc gcacctgctg ccaaagcgga cggcaaatag gtttatgaaa ccgtttgtgc
    15121 cgcctgccat ggcaatgcga ttccgggtat tccccatgta ggcatcaaag ccgattgggc
    15181 cgaccgcatc aaaaaaggca aggacacgtt cgttgcacaa acacgcgatc gaaggtttca
    15241 atacgatgcc cgcaaaaggc ggtcgcggcg atttgagcgc tgatgaagtg aaagctgcgg
    15301 ttgactatat ggtcaaccag tcaggcggca aattctaact tgactgagtt tccgatatta
    15361 tcggttatga aaatccagat tcagtattga atctggattt tttgttttaa cgggcatccg
    15421 gttttaaacg cagactgcct atttgttgta atgaattttg actttagtca taaagaatta
    15481 gtctttattg tttataatcg cgcatcatca cgatagccct attttcaaaa ggacgattaa
    15541 tggatacaca aatcaaaact gaagccggtc agcagaataa ccggcgttat ctgaccgttt
    15601 ggcggtggca tttttatgcc ggtctgctgg ttatgccttt tcttaccctg cttgccgtta
    15661 cgggtttggg tatgctgctg tttgccaata ttaccggtaa ggagggcgag cggattcatg
    15721 ttgtgccgca ggcaacggta caacctctgt ctgttcaggc ggaagcggca cgcagtgccg
    15781 ttaatccgga aacttcgtcc gtcgttcagt atattgcgcc gcgcgccgag gacatggttg
    15841 ccgtgttccg tgtcaacaat gagggtaagg caacgatggt cgcggtcgat ccttatacgg
    15901 caaaagtagt cagcactatg ccgcgcaatc agggttggta tcacacgatg gatgaaatcc
    15961 acagcgatat gatgctcggt tcgacaggcg attatctttt ggaaacggct gcatcgctga
    16021 cgattatcat gataatcagc ggtttgtacc tttggtgggt gaaacggcgc ggcatcaagg
    16081 cgatgctgct gccgtcaaaa ggcagggcgc gttcttggtg gcggaatctg cacggcacgt
    16141 ttggaacttg ggtgtcgttg attttgctgt tgttctgcct gtcgggtatt gcttgggcgg
    16201 gtatttgggg cggcaagttc gtacaggctt ggagtcagtt ccctgccggt aaatggggtg
    16261 tcgaaccgaa ccccgtttca gtcgtgccga cccacggcga ggtattgaat gacggcaagg
    16321 ttaaggaagt gccgtgggtt ttggagctta cgcctatgcc tgtttcaggg acgactgtgg
    16381 gcaaagacgg cattaaccct gacgagccga tgacattgga aaccgtcgac cgctttgcgc
    16441 gggaaatcgg tttcaaaggg cgttatcagt tgaatttgcc caaaggcgag gacggcgtat
    16501 ggactttgtc gcaggattct atgagttacg acatgatcag cccgtttgcc gaccgcacgg
    16561 tacatatcga ccagtacagc ggcaaaatcc ttgccgacat ccgttttgac gattacaacc
    16621 cgttcggcaa atttatggcg gcaagcattg cgctgcatat ggggactctg ggctggtgga
    16681 gcgtgttggc gaacgtcttg ttctgccttg ccgtcatttt tatcggtatc agcggctgcg
    16741 tgatgtggtg gaaacgccgt ccgaccggag cggtgggcat cgttccgccg gcgcagaaag
    16801 tcaagctgcc ggtttggtgg atgatggcat tgccgctatt ggcaatcgca ctgctcttcc
    16861 cgaccgcact gcttgccatt gccgtgattt ggctgttgga tacgctgctg ttgtcgcgga
    16921 ttcctgtttt gaggagatgg tttaaatgac caaatgccgt ctgaaaggtt cagacggcat
    16981 tttgtttgaa ggcggacggg ggaaaggcta tataatcccg aatacttttc cgcaactttt
    17041 gtcgaaatca tgttttatct gtatcaatcc aaccgtcttg aaacgctggc ggcattgttt
    17101 gcccgcattc aaaaagtcaa accgctgaaa tcggctttac agcccgaaca gattattgtg
    17161 cagagtcagg ggatgcgccg ctatctcaat acctgcctcg cccgcgattt gggcgtggcg
    17221 gcgaatttgt cgttcagcct gcccgccggc ctgacgtgga agctgatgaa aaaactgatt
    17281 cccggtattc cggaactcag cccgtttgca cccgaagtca tgcgctggcg gctgctggat
    17341 ttgttccgca gtgaagcatt ccggaatacg gcagaatttg aagatgtgag gaatgtgctg
    17401 caagactatc tgggcagcgg cgaatcggca gattaccagc ttgcgggaca gcttgcggac
    17461 atattcgacc aatacctcgt gtaccgtcct cagtggatag acgcttggca gcagggcagg
    17521 cggctcggtt tgggcgacga cgaaatctgg cagtccaaac tgtggcgtta cctcgacgat
    17581 ggcaggcaga gcgcgccgca ccgtgtcgcg ttgtgggaaa agctgcttgc cgcgttggac
    17641 aaggataagc tgcccgagcg ttatttcgtg ttcggcattt ccacgatggc gccgatgtat
    17701 cttcagcttt tgcacaagct gtccgaacat tgcgatgtgt tcgtgttcgc actcaatccg
    17761 agcgggatgt attggggcaa cgtcatcgaa gcggcgcaaa tcctcaaagg cggcggcgat
    17821 cccgatttaa ctcaggcagg gcatccgctg ctcgcctcat tgggcaagca ggggcgcgac
    17881 tttttcgact ttttgaacga aatggaaata gaaggagaaa cgccggtatt tgaagaaggc
    17941 gggcgcgata cgcttttgca cgccctgcaa accgatattc agaacctgaa aatgccgtct
    18001 gaaaatgtgg gaagcgtcaa cacgggcgac ggctcgatac gcatcgtctc cgcacacagc
    18061 cccttgcgcg aattgcagat actcaaagac aagctgttga aaattctgca tgaacatccc
    18121 gattggcagc cgcatgatat cgccgtatta accccgcaca tcgaatccta tacgcctttt
    18181 atcgaagccg tgttcggaca ggcgcagccc ggtgcgcagg cattgccgta ttccgtttca
    18241 gacgtgaaaa tcagccgccg acaaccgctg tttcatgctt tgtcatgcct gttcgacttg
    18301 ttggaaagcc gatttgaagt cgataaagtg cttgtgcttt tagaaaccgc acccgtgttg
    18361 cgccgtttcg gactgactga ggacgatttg ccgcttttgc acgatatggt tgccgatttg
    18421 aacgttcact ggggtttgga cggagaaatg cgcggcggca cggatcagct gtttacctgg
    18481 aagcaggcgg tagaacgcat gatattgggc tggatgctgc ccgaaggcgg caacccgatg
    18541 tggcaggatg tcagcgcgtg gtatgccgac gtgaaccaaa ccgccctgtt cggacgtttt
    18601 gccgccctcc tcgaaaccct gtcggatatt gtacggatat ggcggcagcc cgcaacgatc
    18661 ggcgaatggg tggcgcgttg ccgggatttg cttgaaacat tgttccaagc tgaagccgat
    18721 gaccaaaagt cagtccaaaa ccttgaaaac gaatgggtca aatggcaggc ggaaacagaa
    18781 ttggcgcaat tttccggaca gttgccgcaa caaaccgtca tccgccatat ccgacgtttc
    18841 ctcgacagcg aaagcgaggc aggcttttta cgcggcggca tcaccttttg cagtatggtg
    18901 ccgatgcgga gcctgccgtt caaagtcatc tgcctgttgg gtttgaacga cggagatttt
    18961 ccccgcaata ccaaagccgc cgtattcgac ctgattgcca aacatcccgc caaaggcgac
    19021 cgcgcccgcc gcgatgacga ccgctacctc ttcctcgaag ccctcatcag cgcgcgcgaa
    19081 atcctctacc tgtcctacat cgggcgcgac atccgcaaag acgaagagct tgctccgtct
    19141 tcgctgttgg gcgaactcat cgacacagtt gccgctatgg cgggcatcgg tagccgccaa
    19201 cttgcacaaa actggataga acagcatccg ctgcaagcct tctcgcgccg atattttcaa
    19261 gaaggcggac gttcagacgg catattcggc acgcgtaccg actacgccac tgcgctcgga
    19321 caaacgcccg aaccgccgca accctttttc gaccaacccg tagaaaacgc cgaacctgtt
    19381 gccgaaatcg gacaggacga atttatccgt ttctggcgca accccgtcaa agtatggctt
    19441 cagcagcagc ttgcgtggag cgaaccccat atcggcgaag cctgggagcc tgccgaaccc
    19501 ttcgagccgc aacacgccga tcaaatcgcc gaaacctaca tcgaagcacg gcgcgaagga
    19561 cgggattttg cccaaaccgc cgcccgcatc ggggcggaaa gcctcctgcc gtcgggagag
    19621 ttgggcagac tttggcagca ggacttccaa accgctgcca aacaaatcga cacggcggtt
    19681 ttaaacagtc ccaaacttcc tccgctgccc tacgccatac cgtcggacgg gcaaatcctg
    19741 aaaggcagct tgggcaatct gtaccgctgc ggacaagtgt tttacgccta cggcaaaccc
    19801 aacgcgccgc aacgtatcgc ttttttgttg gaacacctga tattttgcgc cgttatgccg
    19861 tctgaagccg aaacgcggca aacctttatc gtccaatccg gagaaaccga aatattggcg
    19921 gaaatcgcgc aagacagggc attgcagcta ttgtcggaat ggatggcgtt tttcaatatc
    19981 gggcaaaacc gcccgctgcc gttttttgcc aaaacctcgc ttgccaccgc cgaagcgttt
    20041 gcccaaaaac aagattggga agccgccctg aaaaaagccc aaaccgccta ccacggcagc
    20101 aaagtcagca aagggcagaa agactatacc gaagtcgccc tcgtgttcgg caacgcaagc
    20161 caaaacccgc tcgaacagcc cctgtttgaa aacctcgtcc gtctgcttgc cgacacgctt
    20221 gccgcagcgg aaaaaaagga agaggccgga gaagcctgac gggatggaaa ggcaaatgcc
    20281 gtctgaaagc gtttaagcat ttcagacggc attttgttta tcgtgtttaa ggtgcgggat
    20341 agaatgtggg cgcgccggga ccgacgggca ggcccaaaac aaatacccaa atgcagaata
    20401 aggcaatcca cgcaatcaag aagaaagcgg aatacggcaa catcatagaa atcagcgtac
    20461 ccacgcccgc atcttttttg tatttgatca ccgtcgccat aatcagcccg aaataactca
    20521 tcatcggcgt aataatattg gtaacggaat caccgatgcg gtaagcggct tgaatgactt
    20581 cgggcgcgta gccggccaac atcagcatag ggacgaaaat cggcgcagtt accgcccatt
    20641 gcgcggaggc ggagcctatc atcagattga taaaagcaca aattaaaata aaaccgataa
    20701 acaacacgct gccgcccaag ccgacttctt ttaagaacgt cgccccttta acggcaatat
    20761 attgcccaat attcgtccaa ttaaaaaatg cgacaaactg tgcggcaaaa aagatgatga
    20821 ccaaataaag ccccagagta ctcatcgatt cggccatcgc attaacgact tcctgttcgc
    20881 cgcgcaaact tcgggttacc cggccataaa caatgcccgg cagcgcaaac aacaagaaaa
    20941 taaaaacaac aatcgatttt aaaaacggcg aaccggaaac caatcctgtt tcaggatgac
    21001 gcaaaatacc gtcggcaggg acgatgctcc aagccaatag ggcggataag gcaacaaaca
    21061 ccacgccagc ccaaattaat cctttatatt ccaaaggcgt gatttcattg gaatgccgaa
    21121 tgtctttttc ttcttgtgac aaatctgatt gataagggcc caattgcggt tcgacgattt
    21181 tttcagtaac aaaataacca atcaaagcaa tcacaaacgt actggctacc ataaaaaacc
    21241 agttggcttc agggcctacg acgtagtcgg gatggatgat ttgcgccgcc tgttgggtga
    21301 tgcctgccaa gagcggatcg attgtgccta agaacagatt ggccgaataa ccgcccgaaa
    21361 cgccggcgaa agccgcagcc aaaccggcaa gcggatggcg gccgagcgaa tgaaagatga
    21421 cggcggacaa agggattagg acgacataac ccaattcaga agccgtatta gacaaaatcc
    21481 ctgtaaaaac aaccataaaa gtggtaagtt tgcgtggcga ttttgtgagc aataagcgca
    21541 tcaatgcaga aatcaagccc gatttttccg caatccccac gcccaataaa gaaaccaaca
    21601 ccgttcccaa cggcgcgaaa ccggtgaaat ttttaacggt atgcgtcagg attttgatca
    21661 aaccgtcggc attgagcagg ctgacaacgt aaatcaaacc gtcatcggca cgtcctttcg
    21721 caccaacagg gcgcggatcg gggacggata gtccgaaata cgcaccgacg gcagaggcaa
    21781 tcagcaataa cacaatgaaa ataataaaaa gcgtaaccgg atgcggcaac atattgccca
    21841 gccattcgac tgtgcgtaaa aatcgcccgc cccgccgcgt atcggtttga ttcatctgtt
    21901 tctccttaaa gaaatatatc gtgtgcatta aatttttgaa tgtaacatga agtgttttgt
    21961 aagcaaacta aaataatcgc atatgtatat aaccgcaata gattaaaaca gaaaatgccc
    22021 tcatcgttat tctcataaag gcgggaatcc gaaagctcga cgttaaagcc ttatcaggaa
    22081 tgactggaac tcaaaaaacg gtttcccgct ttcgcggaaa tggcgggatt ttcggttttt
    22141 atttgcaaaa atgccggaga agaatgcagt ttacattttg aatggtattg ttattaattg
    22201 taaataaata ttggaaggta aatgatatga aaaatgaaat tcaaaaaatt atggacaaat
    22261 atgatccctg gcatgaagat gattttgagt cgtatgaaga cattgccaga gatgtatcgc
    22321 tgatgacaga taaaacgttc attgaacatt atttgttaga agtttattca gaagaaaacg
    22381 gacattttga ccaagagaat gtccatgcaa tgatagaaga aattaaaaat gcaatttagt
    22441 gaatatgaga cctttgcaaa aatagtctgt taacgaaatt tgacgcataa aaatgcgcca
    22501 aaaaattttc aattgcctaa aaccttccta atattgagca aaaagtagga aaaatcagaa
    22561 aagttttgca ttttgaaaat gagattgagc ataaaatttt agtaacctat gttattgcaa
    22621 aggtctcaat atgtattttt agaagaaggc tttgtgtcat tttgatgccg tctgaaaggg
    22681 tttgttcgtt tcagacggca ttgttaccaa ggcttgatta tttccggcgc aggcagggat
    22741 ggctttccaa gttgtccatc attatcccga tgatgcgcgg ggcggttttg cccaaatcga
    22801 aacgttcgca ggaagagaac caccgccaaa tcagcccgtc caaggttgat ttgatgaaga
    22861 taactgccgt ttccttgtcc aaatcgtcag ccaaatcctg attttccacc gcttcggtca
    22921 aaacggcggt aattttctcg cgccagattg cctgatgctt gcgggcaatg gcgataacgg
    22981 cggcgttttg ctccgtgtgt tcgcatttta aaaacaggat gttgtggaat ttgtagtaga
    23041 tgtcgttgct ttgcagccgc tcgaaaaagt gcagcagcgt gtggcggaat accgcccaag
    23101 accctccttc ggcatcttcg gcatcttgcg cgatgcagtt ttcgatgtcg tcgcagatac
    23161 gttggaacag cgcgtcgaac aagtcttcct tatttttgaa atgccaatag agcgcgccgc
    23221 gcgttacgcc ggcggcttgg gcgatttcgt tgagcgaggt gcgcgcaatc cctttgcggt
    23281 aaaaggtttc caaggcggca agcatcaggt gttctttggt ttttaaggct tcggttttgg
    23341 tttttctcat aatggcggtt tcgtttcggg tcggtttgat gagggcggat tataaaaaag
    23401 actttgtaac catgcaatcg tgtatgtata atgaaaccca tgaaattgag actacatctc
    23461 aactttgaaa acccatgaaa cctgcttcgc aacccgtttg aacatcgggt tggcgaagca
    23521 ggcggttttt atatcctgaa atatagtgga ttaaca
//
LOCUS       DAWFHY010000024        22692 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.024, whole genome shotgun sequence.
ACCESSION   DAWFHY010000024 DAWFHY010000000
VERSION     DAWFHY010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22692
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.024"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(322..798)
                     /gene="greA"
                     /locus_tag="WGQ68_000849"
     CDS             complement(322..798)
                     /gene="greA"
                     /locus_tag="WGQ68_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEZ0714685.1"
                     /translation="MQKIPLTVRGAELLKQELQQLKSVARPEVIEAIAEARSHGDLSE
                     NAEYEAAKERQGFIEGRISELEHKLSVAHIINPTEIHAEGKIVFGTTVTLEDLETEEH
                     VTYQIVGEDEADIKQGKIYVGSPIARALIGKEEGDTAEVQAPGGVREYDIIEVRYI"
     gene            1190..2491
                     /gene="aroA"
                     /locus_tag="WGQ68_000850"
     CDS             1190..2491
                     /gene="aroA"
                     /locus_tag="WGQ68_000850"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEZ0714686.1"
                     /translation="MTESVRLPVARLKPSTVALPGSKSISNRTLLLAALSDNACEIHS
                     LLKSDDTDRMLEALDKLGVEIEYLAEDRLKVHGTGGRFPNRTADLFLGNAGTAFRPLT
                     AALAVLGGDYHLHGVPRMHERPIGDLVDALRIAGADVEYLGKEHYPPLHIGKRQDNGE
                     RVIPIKGNVSSQFLTALLMALPLTGQAFEIRMVGELISKPYIDITLKLMAQFGVQVIN
                     EGYRVFKIPADARYHAPEHLHVEGDASSASYFLAAGLIAATPVRVTGIGANSIQGDVA
                     FARELEKIGADVVWGENFVEVSRPKERAVQAFDLDANHIPDAAMTLAIVALATGQTCT
                     LRNIGSWRVKETDRIAAMANELRKLGAKVVEEAEAIHITPPKTLTPDAVIDTYDDHRM
                     AMCFSLVSLLGVPVVINDPKCTHKTFPTYFDVFSSLTETTE"
     gene            complement(2534..3067)
                     /locus_tag="WGQ68_000851"
     CDS             complement(2534..3067)
                     /locus_tag="WGQ68_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEZ0714687.1"
                     /translation="MFPPDKTLFLCLSALFLASCGTTSGKHRQPKPKQTVRQIQAVRI
                     SHIDRTQGSQELMLHSLGLIGTPYKWGGSSTAIGFDCSGMIQFVYKNALNVKLPRTAR
                     DMAAASRKIPDSRLKAGDLVFFNTGGTHRYSHVGLYIGNGEFIHAPSSGKTIKTEKLS
                     TPFYAKNYLGAHTFFTE"
     gene            complement(3275..4801)
                     /locus_tag="WGQ68_000852"
     CDS             complement(3275..4801)
                     /locus_tag="WGQ68_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003765285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HEZ0714688.1"
                     /translation="MKTRSLLPLLFLLLCSCAAWLPPLDERTESRHFDTASPVRLDKV
                     LHIRHFPSDSRLSDIYLLDDPHEAFAARAALIESAEHSLDLQYYIWRNDISGRLLFNL
                     VYLAAERGVRVRLLLDDNNTRGLDDLLLALDSHPNIEVRLFNPFVLRKWRALGYLTDF
                     PRLNRRMHNKSFTADNRATILGGRNIGDEYFKVGEDTVFADLDILATGSVVGEVSHDF
                     DRYWASHSAHNATRIICSGNIGKGLQALGYNDETSRHALLRYRETVEQSPLYQKIQAG
                     RIDWQSVQTRLISDDPAKGLDRDRRKPPIAERLQDALKQPEKSVYLVSPYFVPTKSGT
                     DALAKLVQDGIDVTVLTNSLQATDVAAVHSGYVKYRKPLLKAGIKLYELQPNHAVPAT
                     KDKGLTGSSVTSLHAKTFIVDGKRIFIGSFNLDPRSARLNTEMGVVIESPKIAEQMER
                     TLADTTPEYAYRVTLDRHNRLQWHDPATRKTYPNEPEAKLWKRIAAKILSLLPIESLL
                     "
     gene            complement(4911..6317)
                     /locus_tag="WGQ68_000853"
     CDS             complement(4911..6317)
                     /locus_tag="WGQ68_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0714689.1"
                     /translation="MEKNTLSARAPSPWLPLLLAIAIFMQMLDATILNTALPEIATDL
                     DESPLDMQLAVVAYTLTVALLIPLSGYLADRFGTKKVFFGSIAVFMLGSALCAASGSL
                     FELTLSRVVQGIGGSMLVPIPRLTILRMYDKSKLLNAINYAVMPALIGPVLGPLAGGY
                     LVEYASWHWIFLLNLPIGLLGFILGRNIMPDVKGNDTALDFKGYLTFSAAACLLLLAA
                     ESLSHALPPYFALLPLCGGLLFARRYFRHMKTTSKPIYSADLFLIRTFRLGLAGNLFS
                     RLGISSIPFLMPLMFQVAFGFGASLSGWLVAPVALSSLLVKPLIAPLMKRFGYRTVLL
                     WNTKLLAAFIMLLALPDGNSSLWIWIFLSLAIGACNSLQFSAMNTLTLADLRPQQTGS
                     GNSLMAVNQQLAISMGIVVGALILKNWTFLIPASSGLHSAFRMTLLSIGGITLASSLV
                     FNRLHVSDGTNLTRNTPS"
     gene            6601..7380
                     /locus_tag="WGQ68_000854"
     CDS             6601..7380
                     /locus_tag="WGQ68_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HEZ0714690.1"
                     /translation="MSATIPPKIIRYDSNPTDVYFFGTCVLDLFMPEAGMDAITLIEQ
                     QGIRVHFPMAQSCCGQPAYSSGHPTEAFDVAKAQLDLFPENWPIVVPSGSCGGMMKHH
                     WPTLFKGSEYEERAVDCAGRIIEFTHFLLAIGFKPEDKGEPLKVAVHTSCAARREMNV
                     HLSGWQLIDGMENVERIVHDHESECCGFGGTFSVKQADISGAMVTDKVAALKETGATE
                     IISADCGCMMNIGGKIAKDEPDMPRPKHIASFLLERTGGKA"
     gene            7377..8078
                     /locus_tag="WGQ68_000855"
     CDS             7377..8078
                     /locus_tag="WGQ68_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HEZ0714691.1"
                     /translation="MSARENILAKLKKADALPMEEPAVFDYYREKGVSWDSEAERLKH
                     WAAAMRAVKTEIYWVTKSNWMQVFREAAEGKGLKNILLPLATEHGQIARAALADSNIE
                     PIAFEREIDTWKTEFFTNISAGFSGAQCGIARTGTLMLFSSPEEPRTLSLVPPVHFCL
                     FDTSKMYNEFHNAVEGEKLVENGMPTNVFLISGPSKTADIQLTLAYGAHGPRDLVILA
                     ILPDHISPADLEENA"
     gene            8075..9529
                     /locus_tag="WGQ68_000856"
     CDS             8075..9529
                     /locus_tag="WGQ68_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HEZ0714692.1"
                     /translation="MTTQTIKFHMKPETFKQNAAISLQDKPLRKSLRTAMDMLMTKRK
                     AVLTDEEELQSLRDLCEHIRQRSLSKLPALLEQLEENLTKLGVKVHWAETPAEACQII
                     HDIITAKNGKLMVKGKSMVSEEIELNHYLEAKGIKAVESDLGEFIVQMAGEKPTHIVM
                     PAIHKTKEQVSELFHQNLGTPLTDDVDQLTGFARKALRDIYSTADVGLSGVNFAVAET
                     GTLCLVENEGNGRLSTTVPPVHIAITGIEKVVAKLSDIPPLYSLLPRSAIGQNITTYF
                     NMITGPRRSEELDGPQEMHLVLLDNGRSQAYAEDQMRRTLQCIRCGACMNHCPVYTRI
                     GGAAYGTTYPGPIGEIISPHLLGLDATRDLPTACTMCGACVEVCPVRIPITEQMQRLR
                     VEAQRSPTETVPHPIRGQGASHTFGEQMAWRTFNGIFSGSKTYRAFGWAATKFRNLTP
                     RKQLGWTQNRVPMKPAKKTLHELMAEKMRQKEQA"
     gene            complement(9712..10197)
                     /gene="purE"
                     /locus_tag="WGQ68_000857"
     CDS             complement(9712..10197)
                     /gene="purE"
                     /locus_tag="WGQ68_000857"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEZ0714693.1"
                     /translation="MIQIGIIMGSNSDWPVMRQAAQFLEEFGVEYEARVVSAHRTPDL
                     MFQYAETARARGIKAIIAGAGGAAHLPGMVAAKTTVPVLGVPVPSKYLRGEDSLLSIV
                     QMPKGVPVATFAIGEAGAANAALFAVSMLANENPELAQKLADFRAKQEQTVLNMELEQ
                     I"
     gene            complement(10245..10961)
                     /locus_tag="WGQ68_000858"
     CDS             complement(10245..10961)
                     /locus_tag="WGQ68_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system YbgF family protein"
                     /protein_id="HEZ0714694.1"
                     /translation="MKTKLPLFIIWLSVSAACSSPVSRNIQDMRPEPQAEAGSSDAIP
                     YPVPTLQDRLDYLEGTLVRLSNEVETLNGKVKALEHAKTHPSSRAYVQKLDDRKLKEH
                     YLNTEGGSASAHTVETAQNLYNQALKHYKSGRFSAAASLLKGADGGDGGSIAQRSMYL
                     LLQSRARMGNCESVIEIGGRYANRFKDSPTAPEAIFKIGECQYRLQQKDIARATWRSL
                     IQAYPSSPAAKRAAAAVRKR"
     gene            complement(10961..11629)
                     /locus_tag="WGQ68_000859"
     CDS             complement(10961..11629)
                     /locus_tag="WGQ68_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEZ0714695.1"
                     /translation="MTTHLSNVAPDLQNYLNAIGEPEHPVLTRLREKTGHHRMGKMAI
                     AREQAAVLVWLAKLIRAEKYLEIGVFTGYSSTALALALPEHGRITACDINVSFTDTAR
                     QVWNEAGVAHKISLHLQPALLTLDDLIAQGEAGSYDLALIDADKPPTPRYFERCLKLV
                     RQGGIIAIDNILLNGRVMREAASDAPPSVGILKDFNQNLPNDTRIVPITLPVGDGLTL
                     LLKK"
     gene            complement(11640..11825)
                     /locus_tag="WGQ68_000860"
     CDS             complement(11640..11825)
                     /locus_tag="WGQ68_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3460 family protein"
                     /protein_id="HEZ0714696.1"
                     /translation="MYHYQSEATQFLNRLIEEKPELEQQRLENRGLLWDVELNPEEQE
                     NFEAAKVAKKPYTYYQD"
     gene            11968..13944
                     /gene="mutL"
                     /locus_tag="WGQ68_000861"
     CDS             11968..13944
                     /gene="mutL"
                     /locus_tag="WGQ68_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEZ0714697.1"
                     /translation="MPRIAALPDHLVNQIAAGEVVERPANALKEIVENSIDAGATAID
                     VELDGGGIRLIRVSDNGSGIHPDDIEHALHRHATSKIKTLNDLEHVASMGFRGEGLAS
                     IASVSRLTLTSRQEDSSHATQVKAEDGKLSSPTAAAHPVGTTIEAAELFFNTPARRKF
                     LKSENTEYAHCATMLERLALAHPHIAFSLKRDGKPVFKLPAQSLHERIAAIVGDDFQT
                     ASLEIDSGNGALRLYGAIAKPTFAKGKTDKQYCFVNHRFVRDKVMLHAVKQAYRDVLH
                     NALTPAFVLFLDLPPEAVDVNVHPTKTEIRFRDSRQVHQLVFHTLNKALADTRADLTE
                     SVGNAGEVLHDITGVTPAPMPSENDSENLFNRASDYPTGNKPDTRNAFGASGKTAPMP
                     YQSAYAPQQRSLSLRESRAAMNTYAELYKKTDDIDLELSRFEQARFGNMPSETPASQT
                     DTPLSDGIPSQSELPPLGFAIAQLLGIYILAQAEDSLLLIDMHAAAERVNYEKMKRQC
                     QENGRLQSQRLLIPATFAASHEECAALADYAETLAGFGLELSDMGGNTLAVRAAPAML
                     GKSDVVSLARDVLGELAQVGSSQTIEEHENRILATMSCHGSIRAGRRLTLPEMNALLR
                     DMENTPRSNQCNHGRPTWVKLTLKELDALFLRGQ"
     gene            14039..16168
                     /gene="dnaX"
                     /locus_tag="WGQ68_000862"
     CDS             14039..16168
                     /gene="dnaX"
                     /locus_tag="WGQ68_000862"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEZ0714698.1"
                     /translation="MAYQVLARKWRPKTFADLVGQEHVVKALQNALDEGRLHHAYLLT
                     GTRGVGKTTIARILAKSLNCENAQHGEPCGVCESCTQIDAGRYVDLLEIDAASNTGID
                     NIREVLENAQYAPTAGKYKVYIIDEVHMLSKSAFNAMLKTLEEPPEHVKFILATTDPH
                     KVPVTVLSRCLQFVLRNMTAQQVADHLAHVLDSEKIAYEPPALQLLGRAAAGSMRDAL
                     SLLDQAIALGSGKVAENDVRQMIGAVDKQYLYELLTGIINQDGAALTAKAQEMAACAV
                     GFDNALGELAILLQQLALIQAVPNALAHDDPDSDILHRLAQTISGEQIQLYYQIAVHG
                     KRDLSLAPDEYAGFMMTLLRMLAFAPLAAASCDANAVIENTELKSPSAQTAEKETAAK
                     KPQPRPEAETAQTPVQTASAAAMPSEGKTAEPVSNQENNDVPPWEDAPDEAQAAAGTA
                     QTSAKSIQTASEAETPPENQVSKNKAADNETNAPLSEVPSENPIQTTPNNEALETEAF
                     AHEAPAKPFNGYSFPDDDCPVEDNAEIPPPDWEHAAPADTAGGGADEEAEADGIGGNN
                     TPSAPPPEFSTENWAAIVRHFARKLGAAQMPAQHSAWTEYHPDTGLMVLAMTAEARAT
                     ADKKRLDKIRDTLAQAYGLQLTLQTQDWQDESGRETPAMQDKRVQAEDRQKAQALLEA
                     DPAAQKILQAFGAQWQPESLELAANRP"
     misc_feature    15228..15267
                     /gene="dnaX"
                     /locus_tag="WGQ68_000862"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01843"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="This ribosomal frameshift element is known only in
                     the genus Neisseria; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01843"
     gene            16249..16584
                     /locus_tag="WGQ68_000863"
     CDS             16249..16584
                     /locus_tag="WGQ68_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEZ0714699.1"
                     /translation="MFGKAGLGGLMKQAQQMQENMKKAQAKLAETEIEGEAGNGLVKI
                     TMTCAHEVRKIDISPDLIQEAADDKEMLEDLILAALKSARDKAEETANKTMGAFAQGL
                     PPGVGDFFR"
     gene            complement(17415..18461)
                     /gene="recA"
                     /locus_tag="WGQ68_000864"
     CDS             complement(17415..18461)
                     /gene="recA"
                     /locus_tag="WGQ68_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEZ0714700.1"
                     /translation="MSDDKSKALAAALAQIEKSFGKGAIMKMDGSQQEENLEVISTGS
                     LGLDLALGVGGLPRGRIVEIFGPESSGKTTLCLEAVAQCQKNGGVCAFVDAEHAFDPV
                     YARKLGVKVEELYLSQPDTGEQALEICDTLVRSGGIDMVVVDSVAALVPKAEIEGDMG
                     DSHVGLQARLMSQALRKLTGHIKKTNTLVVFINQIRMKIGVMFGSPETTTGGNALKFY
                     SSVRLDIRRTGSIKKGEEVLGNETRVKVIKNKVAPPFRQAEFDILYGEGISWEGELID
                     IGVKNDIINKSGAWYSYNGAKIGQGKDNVRVWLKENPEIADEIDAKIRALNGVEMHIT
                     EGKLDETDGERPEE"
     gene            18715..19479
                     /locus_tag="WGQ68_000865"
     CDS             18715..19479
                     /locus_tag="WGQ68_000865"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEZ0714701.1"
                     /translation="MCSCLVVKNTVIGSGRTKIAVPLVARDAAELSAVLEQIKNMPFD
                     IAEFRADFLECAGSIGEVLRHTQTVRDALPDKPLLFTFRRHGEGGSFPCSDDYYFELL
                     DALIESRLPDIIDIELFSGETAVRRAVADAQKNGIAALLCNHEFHRTPPQEEIVCRLK
                     QMEDCGADICKIAVMPQSAEDVLTLLSATLEAKRLAAKPVVTMSMGQTGAVSRLAGQV
                     FGSSITFGSGTQNSAPGQIGVSALRATLDCLENGAD"
     gene            19499..21514
                     /gene="rep"
                     /locus_tag="WGQ68_000866"
     CDS             19499..21514
                     /gene="rep"
                     /locus_tag="WGQ68_000866"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HEZ0714702.1"
                     /translation="MMKLNPQQLEAVRYLGGPLLVLAGAGSGKTGVITQKIKYLIVNV
                     GYLPHTVAAITFTNKAAAEMQERVAKMLHKSQTRGLTICTFHSLGMKILREEANHIGY
                     KKNFSILDSTDSAKIIGELLGGTGKEAVFKAQHQISLWKNDLKTPEDVVQTASNIWEQ
                     QTARVYASYQETLQSYQAVDFDDLIRLPAVLLQQNSEVRNKWQRRLRYLLVDECQDTN
                     TCQFTLMKLLTGAEGMFTAVGDDDQSIYAWRGANMENLRKMQENYPQMKVIKLEQNYR
                     STARILKIANKVIENNPKLFTKKLWSKLGEGEPVKVVACQNEQHEADWVVSQIVKQKL
                     IGGDKTQYADFAVLYRGKHQARIFEEALRGARIPYRLSGGQSFFDKAEIKDVLSYVRL
                     LANPNDDPAFLRAVTTPKRGIGDVTLGKLNTYAHEHECSLYEAAQNEEALATLNNTNR
                     QHLQAFMDMFGSYRAKAETGEAGEFINSLLEEIDYENHLMQNEEGKAGEIKWRNVSDL
                     VSWFARKGGEDGKNIIELAQTVALMTLLEGKDEEETDAVSLSTLHAAKGLEYPYVFLV
                     GCEEGVLPHNDSIEEGNVEEERRLMYVGITRAKRQLTLTHCVKRKKQGIWQFPEPSRF
                     IDEMPQEDLKILGRKGGEPIVSKEEGRRNLADIIGRLDNLKKSGAAD"
     gene            complement(21582..22640)
                     /gene="dinB"
                     /locus_tag="WGQ68_000867"
     CDS             complement(21582..22640)
                     /gene="dinB"
                     /locus_tag="WGQ68_000867"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEZ0714703.1"
                     /translation="MSSRKIIHIDMDAFYASVELREQPHLKGRPVVVAWEGARSVICA
                     ASYEARQFGLHSAMSVATAKRLCPQAVYVPPHFDLYRQVSAQIHAVFRRYTDLIEPLS
                     LDEAYLDVTRNFKNIPYASEVAKEIRAAIFAETGLTASAGIAPNKFLAKIASDWRKPN
                     GQFVLPPHKVMAFLETLPLGKIPGVGKVTLKKMQSLGMRTAGDLRRFERGELLNHFGR
                     YGYRLYDLVRGTDERPVKAERERLQISTEITLPEDLPLEQAAGHLPHLAEDLWRQITR
                     KNVEAQSVTLKLKTYDFRIITRTLTYSSVLPDCAALLQAAQMLIARVPPQTEDAFRLI
                     GIGVGHLVPKNQQQDLWA"
BASE COUNT         5425 a         6299 c         6175 g         4793 t
ORIGIN      
        1 ggaatgacgg gattttaggt ttctgatttt ggttttctgt ttttgaggga atgacgggat
       61 gcaggttttc ttaaccctgc gtcctagatt cccgcttttg cgggaatgac ggcgacaggg
      121 ttgctgttat agtggatgaa caaaaaccag tacggggttg tctcgcctta gctcaaagag
      181 aacgattctc taaggtgctg aagcaccaag tgaatcggtt tcgtactatc tgtactgtct
      241 gcggcttcgt cgccttgtcc tgatttttgt taatccacta taatttcctg cgtgtgtcgg
      301 gtgtatcgaa atcaagccga atcaaatata tcggacttcg ataatgtcgt attcgcgcac
      361 gccgcccggg gcttggactt ccgccgtatc gccctcttcc ttgccgatta aggcgcgggc
      421 aatcggtgag ccgacataga ttttgccctg tttgatgtcg gcttcgtctt cgccgacaat
      481 ttgataggta acgtgttctt ccgtttccaa atcttccagc gtaaccgtcg taccgaacac
      541 gattttgcct tcggcgtgga tttcggtcgg attgatgatg tgggcaacgg aaagtttgtg
      601 ttccagctcg gaaatgcggc cctcgataaa gccttggcgt tctttggcgg cttcgtattc
      661 ggcgttttcg gacaaatcgc cgtgcgatct ggcttcagca atcgcttcga tcacttcggg
      721 gcgtgccacg cttttgagct gctgcaattc ctgtttcagc aattccgcac cgcgtacggt
      781 cagggggatt ttttgcatcg gtcttttttc tccatattcc ggcacaccgt tttgcggcag
      841 caagcatacc gcgtaccgtc ttgttttgtg cgtccggata ttaaaataaa aatacaagcc
      901 gcccggaaaa tcggcggctt gtctgtcgtt gaacagcggc tattctacca aattctatga
      961 aattggcaat cgtgccgcgc cgccggcaaa cgcgccatgt ccgcaacaaa agctgaaaat
     1021 atgccgacaa agaaatttta gaaacaaaaa atttaaaaat aatcaatttt cggcataaaa
     1081 aaccacattt acggacttta aaaccgaaaa tgccaaacct gagatttttc atacggcatt
     1141 tgcaccagta taatgcaggc tgtttttatc tttatattta cgttttgcca tgaccgaatc
     1201 cgtccgcctc cccgtcgccc gcctcaaacc ttccaccgtc gccctgcccg gctccaaaag
     1261 catcagcaac cgcaccctgc tgctcgccgc cttgtccgac aatgcttgcg aaatccattc
     1321 cctgctcaaa tccgacgata ctgaccgtat gctcgaagca ctcgataaac tcggcgttga
     1381 aatcgaatat cttgccgaag accgtctgaa agtgcacggc acaggcggac gcttccccaa
     1441 ccgcactgcc gatttgtttt taggcaacgc gggaacggcg ttccgtccgc tgactgccgc
     1501 gcttgccgtt ttgggcggcg attatcatct gcacggcgtg cctcgtatgc acgaacgtcc
     1561 tatcggcgat ttggtcgatg cgctgcggat tgccggcgcc gatgtcgaat atctcggcaa
     1621 ggaacactat ccgccgcttc atatcggcaa acgtcaagac aacggcgaac gcgtgattcc
     1681 gattaaaggc aatgtgtcca gccagtttct gaccgccctt ttaatggcgt tgccgctgac
     1741 cgggcaggcg tttgaaatcc gtatggtcgg cgagttaatt tccaagccct atatcgacat
     1801 tactttaaaa ctgatggcgc aattcggcgt acaggttatc aatgaaggct accgcgtctt
     1861 caaaattccc gccgatgcgc gctaccacgc gcccgaacac ttgcacgtcg aaggcgatgc
     1921 ctccagcgcg tcctacttcc tcgcagccgg tttgattgcc gccacgcccg tccgcgttac
     1981 cggtatcggc gcaaacagca tacagggcga tgtcgccttt gcccgcgagc tggaaaaaat
     2041 cggtgcggac gtggtttggg gcgaaaactt cgtcgaagtc tcgcgcccga aagaacgtgc
     2101 cgtccaagcc tttgatttgg atgcaaacca tatccccgat gccgccatga ccctcgccat
     2161 cgtcgcgctt gctacagggc aaacctgcac gcttcgcaac atcggttcgt ggcgcgtcaa
     2221 agagaccgac cgcattgccg caatggcgaa cgagttgcgc aaactcgggg caaaagtcgt
     2281 cgaagaagcc gaagcaattc acatcacccc gcccaaaacg ctgacacccg acgccgtcat
     2341 cgacacgtac gacgaccacc gcatggcgat gtgtttctcg ctggtttcgc tgttgggcgt
     2401 acccgtcgtc atcaacgatc cgaaatgcac ccataaaacc ttcccgactt atttcgacgt
     2461 gttctcttca ctgaccgaaa caacggaata aggcggcatt ttgccgcgat tccggcgcgg
     2521 cgcggcgggc ggctcattct gtaaaaaaag tatgtgcgcc gaggtagttt ttggcgtaaa
     2581 acggtgtgga gagtttttcg gttttgatgg ttttgccgct gctgggggca tggatgaatt
     2641 cgccgttgcc gatatagagt ccgacgtgtg agtagcggtg tgtgccgccg gtgttgaaga
     2701 atacgaggtc gccggcctta aggcggctgt cggggatttt gcggcttgcc gccgccatgt
     2761 cgcgggcggt gcgcggcagc ttgacgttga gggcgttttt gtaaacgaat tgaatcatgc
     2821 cgctgcaatc gaagccgatt gcggtgctgc tgccgcccca tttgtagggc gtgccgatga
     2881 gtccgaggct gtggagcatg agttcctgcg agccttgtgt gcggtcgatg tggctgatgc
     2941 ggacggcttg gatttgccgg actgtctgtt tgggtttcgg ttggcggtgt ttgccggagg
     3001 tcgtgccgca tgaggcgagg aacagtgcgc tgagacagag gaaaagggtt ttgtcggggg
     3061 gaaacatggt ttttcctttg cgggttcgga tatccgtctg aaggtgtttc agacggtata
     3121 gtggattaat aaaaatcagg acaaggcgac gaagccgcag acagtacaaa cagtacggaa
     3181 ccgattcact tggtgcttca gcaccttaga gaatcgttct ctttgagcta aggcgaggca
     3241 acgtcgtact ggtttttgtt aatccgctat atttctataa taaactttct atgggcagca
     3301 gggataggat ttttgcggcg atgcgtttcc aaagtttggc ttcgggttcg ttcgggtagg
     3361 tttttcgggt ggcgggatca tgccattgca ggcggttgtg cctgtcgagg gtaacgcggt
     3421 aggcgtattc gggtgtggta tcggcaaggg tgcgctccat ctgttctgcg attttggggc
     3481 tttcgataac aacgcccatt tcggtgttga gacgcgcgga acgggggtcg aggttgaacg
     3541 aaccgatgaa gatgcgtttg ccgtccacaa tgaaggtttt ggcgtgcagg ctggttacgg
     3601 agctgccggt caggcctttg tcttttgtgg cggggacggc atggttgggt tgcagctcgt
     3661 agagtttgat gccggctttg agcagcggtt ttcggtattt gacatagccg gaatggacgg
     3721 cggcaacgtc ggtcgcctgt agcgagttgg tcagaacggt aacgtctatg ccgtcctgca
     3781 ccagttttgc cagtgcgtct gtgccggatt ttgtggggac gaaatagggt gaaaccagat
     3841 agacgctttt ttcgggctgt ttgagcgcgt cttgcagccg ctcggcaatc ggcggtttgc
     3901 ggcggtcgcg gtcgagtcct tttgcagggt cgtcgctgat gaggcgggtt tggacgctct
     3961 gccagtcgat gcgtcctgcc tgtatttttt ggtagagggg cgactgttcg acggtttcgc
     4021 ggtagcgcag gagcgcgtgt ctggacgttt cgtcgttgta tccgagtgct tgaagaccct
     4081 tgccgatgtt gccgctgcag atgatgcgcg tggcgttgtg ggcggaatgg cttgcccagt
     4141 agcggtcgaa gtcgtgcgat acttcgccga cgacgctgcc ggtggcgagg atgtccaggt
     4201 cggcgaaaac ggtgtcctca ccgactttga agtattcgtc gccgatattg cgtccgccga
     4261 gtatggtggc gcggttgtcg gcggtaaagg atttgttgtg catgcggcgg ttgaggcggg
     4321 ggaagtcggt caggtagccg agtgcacgcc attttcgtaa gacgaagggg ttgaacaggc
     4381 gcacttcgat attgggatgg ctgtcgaggg cgagcaggag gtcgtccaat ccgcgcgtgt
     4441 tgttgtcgtc caacagcagg cgtacgcgca caccgcgttc tgcggcaagg tacacgaggt
     4501 tgaacagcag tcggccggaa atgtcgttgc gccagatgta gtattgcaaa tcgaggctgt
     4561 gttcggcaga ttcgataagg gcggcgcggg cggcaaaggc ttcgtggggg tcgtcgagca
     4621 ggtaaatgtc ggacaggcgg ctgtcggacg ggaaatggcg gatgtgcagg actttatcca
     4681 aacggacggg ggaagcagtg tcaaaatggc ggctttccgt gcgttcgtcc aagggcggca
     4741 gccatgcggc gcacgagcag agcaggagga acaacagggg aaggaggctg cgtgttttca
     4801 taggaaatcg gttcagacgg cattgcctgt gttttggggt tggtgcgcat ggaagtgcgg
     4861 catcataatc caaacgttga aacgggtaaa agttttgcgt gcggaccgct tcaggacggt
     4921 gtattccgtg tcaggttggt gccgtctgaa acgtgcagcc gattgaatac cagcgatgag
     4981 gcaagggtga tgccgccgat gctgagcagg gtcatacgga aggcggaatg cagacctgaa
     5041 gaagccggta tcagaaatgt ccagttttta aggattaatg cgccgacaac aatgcccatg
     5101 ctgatggcaa gctgttggtt gaccgccatc aggctgttgc cgctgccggt ctgttgcggg
     5161 cgcaaatcgg cgagggtcag tgtattcatg gcagaaaact gcagggagtt gcacgcgccg
     5221 atcgccagcg agaggaaaat ccaaatccac agcgacgagt ttccgtcagg cagggcgagc
     5281 agcatgatga aggcggcaag cagcttggtg ttccaaagca gtaccgtgcg gtagccgaaa
     5341 cgtttcatga gcggtgcaat cagcggtttg accagcagcg aagacagggc gacgggggcg
     5401 accagccaac ccgacaggct tgcgccgaag ccgaaagcga cttgaaacat caggggcatc
     5461 agaaaaggaa tcgagctgat gccgagacgg ctgaacagat tgcctgccag tcccagacgg
     5521 aaagtgcgta tcagaaacag gtcggcggaa taaatcggtt tggacgtggt tttcatatgt
     5581 cggaaataac ggcgtgcaaa cagcagtccg ccgcacagcg gcaacagtgc aaaatacgga
     5641 ggcagcgcgt gcgacaggct ttctgcagca agcagtagca ggcaggcggc agcggaaaag
     5701 gtcagatagc ctttaaaatc cagagcggta tcgttgcctt tgacatcggg catgatgttg
     5761 cgtcccaata taaaccccag cagaccgatg ggcaggttga gcaggaaaat ccagtgccac
     5821 gaagcgtatt cgaccagata accgcccgcc aaaggcccta aaaccggccc gattaatgcg
     5881 ggcataaccg cataattgat ggcgttgagc agcttggact tgtcgtacat acgcaagatg
     5941 gtcagacgcg gtatcggaac cagcatcgaa ccgccgatgc cctgaacgac acgggaaagc
     6001 gtcaattcaa acagcgaacc cgatgcggcg cacaatgccg atccgagcat aaaaacggca
     6061 atcgaaccga aaaagacttt tttcgttccg aacctgtccg ccaaataacc gctcaaagga
     6121 atcagcaggg caaccgtcag cgtataggca acgactgcca gttgcatatc cagaggcgat
     6181 tcgtccaggt cggtggcaat ttcaggcagt gcggtattta aaatggtcgc atccaacatc
     6241 tgcataaaaa tggcaattgc cagcagaagc ggcagccaag gggatggtgc gcgggcggat
     6301 agggtgtttt tttccatagg gcgattgtac cccatccttg tgccgttatt gttttcagac
     6361 gctgtctgaa tgccgtcaga gtcggcatct tgaatgttca caagcaaacg aaccggtatt
     6421 gcattgtaat gataactatt atcgaaaaac atcagattaa ggtacagtaa gcgttatggg
     6481 gtagtttgta agaaaaaccg gattattttt taaaattaga cttgacccgc aacagtcaat
     6541 tacttaaagt aaacgcttac ctttctacag aaaaaaacgg gtttcccgtt atcaaaaaac
     6601 atgagcgcaa ccattccccc aaaaatcatc cgatacgaca gcaatccgac cgatgtctat
     6661 tttttcggca cttgcgtcct tgatcttttt atgcccgaag caggcatgga tgccattacc
     6721 ctaatcgagc aacagggcat acgcgtccat ttcccgatgg cgcaaagctg ctgcggccag
     6781 cctgcctatt catccggcca tccgaccgaa gccttcgatg tcgccaaagc acaactcgac
     6841 cttttccctg aaaactggcc gatcgtcgtg ccgtccggct cgtgcggcgg catgatgaaa
     6901 caccactggc cgacgctgtt taaaggcagc gagtacgagg aaagggctgt ggattgcgcc
     6961 ggccgcatca tcgagtttac ccatttcctg cttgccatcg gtttcaaacc cgaagacaag
     7021 ggcgaaccgc tcaaagtcgc cgttcacact tcctgcgccg cccgccgcga aatgaatgtc
     7081 catctttcag gctggcaact gattgacggt atggaaaacg tcgaacgcat cgtccacgac
     7141 cacgaaagcg aatgttgcgg cttcggcggc acattctccg tcaaacaagc cgatatttcc
     7201 ggcgcaatgg taacagacaa agtcgccgcg ctgaaagaaa ccggcgcaac cgaaatcatc
     7261 agcgcggact gcggctgtat gatgaacatc ggcggcaaaa tcgccaagga cgagccggat
     7321 atgccgcgtc cgaaacatat cgcatccttc ttgttggaac gcaccggagg caaagcatga
     7381 gcgcgcgtga aaatattttg gcaaaactga aaaaagccga tgccttgccg atggaagagc
     7441 ctgcggtttt cgattattac cgcgaaaagg gcgtttcttg ggacagcgaa gccgagcgtc
     7501 tgaaacattg ggctgccgca atgcgcgcgg tcaaaaccga aatttattgg gtgacgaaaa
     7561 gcaattggat gcaggttttc cgcgaagcgg cagaaggcaa gggtttgaaa aacatcctgc
     7621 tgcccttggc gaccgaacac ggacaaattg cccgtgccgc attggcggac agcaatatcg
     7681 aaccgattgc cttcgagcgc gaaatcgata cttggaaaac cgagtttttc acgaacatca
     7741 gtgcgggctt cagcggcgcg caatgcggca tcgcccgcac cggcacgctg atgctgtttt
     7801 ccagccccga agaaccgcgt actttgagtc tcgttccgcc cgtgcatttc tgcctgttcg
     7861 atacgtccaa gatgtacaac gagtttcata atgccgtcga aggcgaaaaa ctggtggaaa
     7921 acggtatgcc gaccaatgta ttcctgattt ccggcccgtc caaaaccgca gacatccaac
     7981 tgacgcttgc ttacggcgcg cacggcccgc gcgatttggt catcctcgcc atcctgcccg
     8041 accacatttc ccctgccgat ttggaggaaa acgcatgact acgcaaacca tcaagttcca
     8101 catgaagccg gaaactttca agcaaaacgc cgcaatttcc cttcaagaca agcctttgcg
     8161 caaaagcctg cgtaccgcga tggatatgct gatgaccaaa cgcaaagccg ttttgaccga
     8221 cgaagaagag ctgcaaagcc tgcgcgattt gtgcgaacac atccgccagc gttccctgtc
     8281 caaattgccc gcgctgttgg agcagttgga agaaaacctg accaagttgg gcgtgaaagt
     8341 acactgggcg gaaacccctg ccgaagcctg ccaaattatc cacgacatca tcacagccaa
     8401 aaacggcaag ctgatggtca aaggtaaatc catggtcagc gaggaaatcg agctgaacca
     8461 ttatcttgaa gcaaaaggca ttaaagcggt agaaagcgac ttgggcgagt tcatcgtcca
     8521 aatggcaggc gaaaaaccga cccatattgt gatgcctgct atccacaaaa ccaaagaaca
     8581 ggttagcgaa ctgttccacc aaaacctcgg tacgccgctg acagacgatg tagaccaact
     8641 gaccggcttc gcccgtaaag cactgcgcga tatttacagc actgccgatg tcggtttgag
     8701 tggcgtaaac ttcgccgttg ctgaaacagg tacgctgtgt ctggtggaaa acgaaggcaa
     8761 cggtcgcttg agtaccaccg taccgcccgt gcatatcgct attaccggca ttgaaaaagt
     8821 ggtggcgaaa ttgtccgaca tcccaccctt gtacagcctg ctgccgcgtt ctgccattgg
     8881 tcagaacatt accacttatt tcaatatgat taccggcccg cgccgtagcg aagagctgga
     8941 cggtcctcaa gaaatgcact tggtcctgct tgacaacggt cgcagccaag cctatgccga
     9001 agaccaaatg cgccgcacct tgcaatgtat ccgttgcggc gcgtgtatga accattgccc
     9061 ggtttatacc cgtatcggtg gcgcggcata tggcacaacc tatcccggtc cgattggcga
     9121 gattatttcc ccgcacctgt taggcttgga tgccacccgc gacctgccga ccgcctgtac
     9181 catgtgcggt gcgtgtgtgg aagtctgtcc ggtacgcatc ccgattaccg aacaaatgca
     9241 gcgtttgcgc gttgaagcgc aacgttcgcc gaccgaaacc gtgccgcacc ccatccgagg
     9301 gcaaggcgca tcgcatacct tcggcgaaca aatggcgtgg cgcacattca acggtatttt
     9361 cagcggcagc aaaacctacc gcgccttcgg ttgggcagcc accaagttcc gcaacctgac
     9421 cccgcgcaaa cagttgggtt ggacgcaaaa ccgcgtgccg atgaaaccgg cgaagaaaac
     9481 cctgcacgaa ctaatggcag aaaaaatgcg ccaaaaagaa caggcataaa aagttgttcg
     9541 caaaaatgcc gtctgaaacc cgaaacaggg cttcagacgg catttgtata gtggattaac
     9601 aaaaatcagg acaaggcgac gaaaccgaag acagtacaaa tagtacggca aggcgaggta
     9661 acgccgtact ggtttaaatt taatccacta tatattcgcg gacggcgggt tttaaatctg
     9721 ttccaattcc atattcaaaa cagtctgttc ttgtttggct cggaagtctg ccagtttttg
     9781 cgccagttcg gggttttcgt tggcgagcat ggaaacggcg aacaatgcgg catttgccgc
     9841 acctgcctcg ccgatggcga atgtggcgac gggtacgcct ttgggcattt gtacaatcga
     9901 taaaagcgaa tcttcgccgc gcaggtattt gctggggacg ggtacgccca aaacggggac
     9961 ggtggtcttg gcggcaacca taccgggtaa atgcgccgcg ccgcccgcac ccgcgatgat
    10021 ggctttgatg ccgcgcgccc gtgcggtttc ggcgtattgg aacatcaaat ccggggtgcg
    10081 gtgtgcggaa acaacgcgcg cctcatattc tacgccgaat tcttcaagaa actgcgctgc
    10141 ctgccgcata acgggccaat cgctgttgct gcccatgatg atgccgattt gtatcataaa
    10201 tcctccttgg tgcggatggg gtaaaaagcg gaaaaatgga aaaactatcg tttgcgcacg
    10261 gctgcggcgg cgcgttttgc cgccgggctg ctcgggtaag cctgtatcag gctgcgccaa
    10321 gtcgcccttg caatgtcttt ctgctgcaac ctgtattggc attcgccgat tttgaatatg
    10381 gcttcgggcg cggttgggct gtctttgaaa cggttggcgt aacgccctcc gatttcgatg
    10441 acggattcgc agttgcccat acgcgccctg ctttgcagca acagatacat actgcgttgc
    10501 gcgatgctgc cgccgtcgcc tccgtccgcg cctttcaaca gggaggcagc ggcagaaaac
    10561 ctgccgcttt tatagtgttt gagtgcctga ttgtagaggt tttgtgcggt ttcgacggta
    10621 tgtgcggatg cgctgccgcc ttcggtattg aggtaatgct ctttcaactt gcggtcgtcg
    10681 agtttttgga cgtatgccct gctggaaggg tgtgttttcg catgctccag tgctttgact
    10741 ttgccgttta aggtttccac ttcgttcgac aggcggacga gtgtgccttc cagataatcc
    10801 aaacggtctt gcagagtggg aacgggatag ggaatagcgt ccgaactacc tgcctctgcc
    10861 tgcggttcgg gccgcatatc ctgaatattg cgggaaacag gggaagaaca ggcggcggat
    10921 acggacagcc aaatgataaa aagcggtaat ttggtcttca ttattttttc agaagcaggg
    10981 tcaagccgtc gccgacgggc agggtgatgg ggacaatccg cgtatcgttc ggcaggtttt
    11041 gattgaaatc tttgaggatg ccgacgctgg gcggcgcatc ggaagccgct tcgcgcatca
    11101 cccttccgtt cagcaaaata ttgtcgatgg cgatgatgcc gccttgacgg acgagtttga
    11161 ggcaacgctc gaaatatcgc ggcgtgggcg gtttgtctgc gtctatcagt gccaaatcgt
    11221 agcttccggc ttcaccctgt gcaatcaaat catccaatgt cagcaatgcg ggttgcaggt
    11281 gcaggctgat tttatgtgcc acaccggcct cgttccaaac ctgacgcgcc gtatcggtaa
    11341 agcttacatt gatgtcgcag gcggtaatcc gcccgtgttc gggcaacgcc aatgcaagcg
    11401 cggtgctgct gtatccggta aatacgccga tttccagata tttttccgca cggatcagct
    11461 ttgccagcca aaccaaaacc gccgcctgtt cgcgcgcaat cgccattttg cccatacggt
    11521 gatgcccggt cttctcacgc agccgcgtca aaacgggatg ttcaggttcg ccgatggcgt
    11581 tcaaatagtt ttgcaggtcc ggtgcaacat tggacagatg ggtcgtcatt tcggcgggtt
    11641 cagtcttggt aataggtata aggttttttc gctacttttg ccgcctcgaa gttttcctgt
    11701 tcttcgggat tgagttcgac atcccacaaa agcccccggt tttccaaacg ctgctgttcc
    11761 aactcaggtt tttcttcaat caggcggttg aggaattgtg tggcttcgga ttggtagtga
    11821 tacatctttg cgctccaatt ttacgggata cggcgtgatt atactggtat tttccaaacg
    11881 ggataaacgg cttttatcaa gaatacaggc agaaagataa ggggttttat tatagaataa
    11941 agcgtttttg caacggaagc ctaccttatg ccacgcattg ccgccctgcc cgaccatctc
    12001 gtcaaccaaa tcgccgccgg cgaagtagtc gaacgccctg ccaacgcctt gaaagaaatc
    12061 gttgaaaaca gtatcgatgc aggcgcaacg gcgattgatg tcgagctgga cggcggcggc
    12121 atccgcctga ttcgcgtcag cgacaacggc agcggtatcc atccggacga catcgaacac
    12181 gcgctccacc gccacgccac cagcaaaatc aaaaccttaa acgatttgga acacgttgcc
    12241 agcatgggct ttcgcggcga aggcttggca agcatcgcct ccgtcagccg cctgaccctg
    12301 accagccgtc aagaagacag ttcgcacgca acccaagtca aagccgaaga cggcaaactc
    12361 agcagcccca ccgccgccgc ccaccccgtc ggcaccacca tcgaagctgc cgaactcttc
    12421 ttcaacaccc ccgcgcggcg caagtttctc aagtccgaaa acaccgaata cgcccactgc
    12481 gccaccatgc tcgaacgcct cgcgctggcg catccgcaca tcgccttctc actcaaacgc
    12541 gacggcaaac cagtgttcaa acttcctgcc caaagcctgc atgaacggat tgccgccatt
    12601 gtcggcgatg actttcagac ggcatccctg gaaatcgaca gcggcaacgg cgcattgcgg
    12661 ctctatggcg cgattgccaa gccaaccttt gccaaaggca aaaccgacaa acaatactgc
    12721 ttcgtcaacc atcgttttgt ccgtgacaaa gtcatgctcc atgccgtcaa gcaggcatac
    12781 cgcgacgtat tgcacaacgc gctgacgcct gctttcgtcc tctttctcga cctgccgccc
    12841 gaagccgtgg atgtcaacgt ccatccgacc aaaaccgaaa tccgcttccg cgacagccgg
    12901 caggtgcacc aactcgtgtt ccacacgctc aacaaagccc ttgccgacac gcgcgccgac
    12961 ctgaccgaaa gcgtcggcaa cgcaggcgaa gtgttgcacg atattaccgg cgttacgcct
    13021 gcaccaatgc cgtctgaaaa cgacagcgaa aatctgttta atcgcgcatc cgactacccg
    13081 acaggcaaca aacccgatac gcgcaatgcc tttggtgcat caggcaaaac cgcgcctatg
    13141 ccctatcagt ccgcatatgc gccgcaacaa cgcagcctgt ccctgcgcga aagccgcgcg
    13201 gcaatgaaca cctatgccga actctacaaa aaaaccgacg acatcgacct tgagttaagc
    13261 cgattcgagc aggcacgttt cggcaatatg ccgtctgaaa cgcctgcttc ccaaacagat
    13321 acgccgcttt cagacggcat cccgtcccaa tccgaactgc cgccgctcgg ttttgccatt
    13381 gcccaattat taggcatcta catccttgcc caagccgaag acagcctgct gctcatcgat
    13441 atgcacgccg ccgccgaacg cgtcaactac gagaaaatga aacgccaatg tcaggaaaac
    13501 ggcaggctgc aaagccaacg cctgcttatt cccgcaactt tcgccgcgtc ccacgaagaa
    13561 tgcgccgccc ttgccgatta tgccgaaacg ctggcaggct tcgggctgga attgtccgat
    13621 atgggcggca acaccctcgc cgtccgcgcc gcacccgcaa tgttgggcaa atccgatgtc
    13681 gtctccctcg cccgcgacgt attgggcgaa ctcgcccaag tcggcagcag ccaaaccatc
    13741 gaagaacacg aaaaccgcat cctcgccacg atgtcctgcc acggctcgat ccgcgccggc
    13801 cgccggctta ccctgcccga aatgaacgcg ctgctgcgcg atatggaaaa cacgccgcgc
    13861 agcaaccagt gcaaccacgg cagaccgacg tgggtcaaac tgactttgaa agaattggac
    13921 gcactgttct tgcgcggaca ataagccgaa agtgctagaa tacgccgccc gagaccgccg
    13981 ttcagacggc attccgacgc accgacagaa acatcacgac cgaaaccaag agaaaaatat
    14041 ggcctatcaa gttctcgccc gaaaatggcg gcccaaaacc tttgccgact tagtcggtca
    14101 ggaacacgtc gtcaaagccc tacaaaatgc cttggacgaa ggcaggctgc accacgccta
    14161 cctgctgacc ggtacgcgcg gcgtaggcaa aaccaccatc gcccgcatcc ttgccaaaag
    14221 cctcaactgc gaaaacgcgc aacacggcga accttgcggc gtgtgcgaaa gctgcaccca
    14281 aatcgatgcc ggacgctatg tcgacctgct cgaaatcgac gccgcctcca acacaggcat
    14341 cgacaacatc cgcgaagtct tggaaaacgc ccaatacgcg ccgaccgccg gcaaatacaa
    14401 agtctatatc atcgacgaag tgcatatgct ttccaaaagc gcgttcaacg ctatgctcaa
    14461 aacgctggaa gagccgcccg aacacgtcaa attcatcctc gccaccaccg atccgcacaa
    14521 agttcccgtt accgtcttga gccgctgcct gcaattcgtc ttacgcaata tgaccgcgca
    14581 gcaggttgcc gaccacctcg cccacgtcct cgacagcgaa aaaatcgcct acgaaccccc
    14641 cgccctgcaa cttttgggac gcgcagccgc cggatcgatg cgcgatgcct taagcctgct
    14701 cgaccaagcc atcgccctag gttcgggcaa agttgccgaa aacgatgtcc gccaaatgat
    14761 cggcgcggtt gacaaacaat acctttacga actgctgaca ggcatcatca accaagacgg
    14821 cgcagccctg accgccaaag cgcaggaaat ggcggcgtgt gccgtcggct ttgacaacgc
    14881 cttgggcgaa cttgccatac tgctgcaaca actcgccctg atacaggcag tgccgaatgc
    14941 cttggcgcac gacgaccccg attccgatat tttgcaccgc ctcgcccaaa ccataagcgg
    15001 cgaacaaatc cagctttact accaaatcgc cgtccacggc aaacgagacc tgagcctcgc
    15061 tcccgacgaa tacgccggct ttatgatgac cctgctgcgt atgctggcgt ttgcgccctt
    15121 ggcggcagct tcgtgtgatg caaacgccgt gattgaaaat accgaactaa aatccccatc
    15181 ggcacaaacc gccgaaaagg aaaccgccgc aaaaaagccc caaccgcgcc ctgaagcgga
    15241 aaccgcccaa acacccgttc agacggcatc cgcagcggca atgccgtctg aaggcaaaac
    15301 cgccgaaccc gtttccaatc aagaaaacaa cgatgttccg ccttgggaag acgcgccgga
    15361 cgaagcccaa gccgcagccg gcacggcgca aacgtcggca aaaagcattc agacggcatc
    15421 cgaagccgaa acgccgcccg aaaaccaagt ttccaagaac aaggcagccg acaacgaaac
    15481 caatgccccc ttgtccgaag tgccgtctga aaaccccatt cagacaacac cgaataatga
    15541 agcccttgaa acggaagcat ttgcgcacga agctcctgca aaacctttca acggttacag
    15601 ctttccggat gatgactgcc ccgtagaaga caacgcggaa atcccgccgc ccgattggga
    15661 acacgccgcc cctgccgata ccgcaggcgg aggcgcggac gaagaagcag aagcagacgg
    15721 catcggcgga aacaacacac cgtccgcccc accgcccgag ttttccaccg aaaactgggc
    15781 agccatcgtc cggcatttcg cccgcaaact cggcgcggcg caaatgccgg cgcaacactc
    15841 cgcgtggacg gaataccatc ccgacaccgg cctgatggtt ttggcaatga ccgccgaagc
    15901 acgcgccacc gccgacaaaa aacgtctcga caaaatccgc gacacccttg cccaagccta
    15961 cgggctgcaa ctcaccctgc aaacccaaga ctggcaggac gaatccggca gagaaacccc
    16021 cgcgatgcag gacaagcgcg tccaagccga ggacaggcaa aaagcacaag cattgctcga
    16081 agccgacccc gccgcacaaa aaatcctcca agcattcggc gcgcaatggc agcccgaatc
    16141 actggaattg gcggcaaacc ggccataaac agatataatg ccgcccgaac ccttcggacg
    16201 gcattgccgt ttccctcatt catttaaaca caaacaggag tattcagtat gttcggaaaa
    16261 gccggattag gcggcctgat gaaacaggcg cagcaaatgc aggaaaatat gaaaaaagcg
    16321 caagccaaac ttgccgaaac cgaaatcgaa ggcgaagcag gcaacggctt ggtcaaaatc
    16381 acaatgacct gcgcgcacga agtacgcaaa atcgacatca gccccgattt gattcaagaa
    16441 gccgccgacg acaaagaaat gctcgaagac ctcatcctcg ccgccctcaa atccgcccgt
    16501 gacaaagccg aagaaaccgc aaacaaaaca atgggcgcat tcgcccaagg actccccccc
    16561 ggagtgggcg acttcttccg ctgatccccg accgtcattc ccacgcaggc gggaatctag
    16621 aacgtagaat ctgagaaacc gttttactcg ataaatttcc gtgccgaggg gtctggattc
    16681 ccgccttcgc gggaatgacg gcatcagttt gcaggattcg gcgtgaacgg taaaaacagt
    16741 gagaatgata agaacgcaaa aacggcaaga atagcgggaa tcggcaggct gaagcccacc
    16801 ctaccattat ttacacatcc gtaccgctta aatgccgtct gaaacttcgt cattcccgtg
    16861 aaaacgggaa tccagccccg tgggagcaga aacttacacc ccgtcattcc cgcgaacgcg
    16921 ggaatccagt aaccgaaaaa ccacaggaat ctatcggaaa aacagaaacc cccgaccgtc
    16981 attcccgcga acgcgggaat ccagtaaccg aaaaaccacg ggaatctatc ggaaaaaaca
    17041 gaaaccctcg accgtcattc ccgcgaacgc gggaatctag aacgtagaat ctgagaaacc
    17101 gttttactcg ataaatttcc atgccaacag gtctggattc ccgccttcgc gggaatgacg
    17161 gcatattttt tgcatttgat ataaagggtc gtttgaattt tgttcagcaa gtgcaaagtg
    17221 ttgcacataa aagggcgtag gcttcatccg taaagtcggc gcaggataga ggcaaagcgg
    17281 gcgtaggtcg ggctgtagca actgtatttt tcaccccgtc gggcaaaaat accaaaactc
    17341 aaatcaagcc gttcggaggc agctcaaaaa acggtacttc gcaacagaag taccgtttat
    17401 cgggatttta agttttattc ttctgggcgt tcgccgtcgg tttcgtccaa ttttccttcg
    17461 gtgatgtgca tttctacgcc gttgagggcg cgaatttttg cgtcgatttc gtcggcaatt
    17521 tcgggatttt ccttcagcca gacgcggacg ttgtctttgc cctgaccgat tttcgcgccg
    17581 ttgtagctgt accacgcgcc ggatttgttg atgatgtcgt ttttcacgcc gatgtcgatc
    17641 aattcgccct cccaactgat gccttctccg tagaggatgt caaactctgc ctgacggaac
    17701 gggggggcga ctttgttttt gatgactttg acgcgggttt cgttgcccaa tacttcttcg
    17761 ccttttttga tggatccggt gcggcggatg tcgaggcgga cggaagaata gaatttcagc
    17821 gcgttgccgc cggtggtggt ttcggggctg ccgaacatta cgccgatctt catccggatt
    17881 tggttgatga acacaaccag cgtgttggtt tttttgatgt gtccggtcag tttgcgcaaa
    17941 gcctgactca tcaggcgcgc ctgcagtccg acgtggctgt cgcccatatc gccttcgatt
    18001 tcggctttgg ggacgagtgc ggcgacggaa tcaacgacta ccatatctat gccgcccgaa
    18061 cggacaagtg tgtcgcagat ttccaaagcc tgttcgccgg tatcgggctg ggacaggtaa
    18121 agctcttcga cttttacgcc gagtttgcgg gcgtaaacgg gatcaaaggc gtgttcggca
    18181 tcgacaaagg cgcacacgcc gccgtttttc tggcattggg cgacggcttc gaggcagagg
    18241 gtggttttgc cggaggattc gggaccgaag atttcgacga tgcgcccgcg cggcagaccg
    18301 ccgactccga gggcaaggtc taatccgagc gatccggtgg aaatgacttc gaggttttct
    18361 tcctgctggc tgccgtccat tttcatgatg gcgcctttgc cgaaactttt ttcgatttgc
    18421 gccagtgcgg cggcaagggc tttgcttttg tcgtctgaca tgggggttac tccggaacaa
    18481 atgcggtatg tgggatgcgg cgcaacacgg gctgcggcgc gggatgtgta tcgttttccc
    18541 gatgtgcggg ctatcggtaa tgctgcttca cggggttgcc attatcgcat atttccttgc
    18601 ttgccgatat gcggcaggac gcggcggctt gtgccggaat ggaatctgga tgccgtctaa
    18661 aaggcggccg gctttgttat aatggcggct gttttttctg tgtgtgcctg ttttatgtgt
    18721 tcctgccttg ttgtcaaaaa taccgttatc ggaagcggac gcaccaaaat cgccgtgccg
    18781 cttgtcgccc gcgatgctgc cgaactttcc gccgtacttg agcaaatcaa aaatatgccc
    18841 ttcgatattg cggagttccg cgccgacttt ttggaatgcg cgggcagcat cggcgaagta
    18901 ttgcgccaca cgcagaccgt ccgcgacgcg ctgcccgaca aaccgctgct gtttacgttc
    18961 agacggcatg gcgaaggcgg ctcgttcccg tgttcggacg attattattt tgaactgctc
    19021 gacgcgctga tcgaaagccg cctgcccgac atcatcgaca tcgagctgtt ttccggcgaa
    19081 accgccgtcc ggcgcgccgt ggcggatgct caaaaaaacg gcatcgctgc cctgctctgc
    19141 aatcatgagt ttcaccgcac gccgccgcaa gaagaaatcg tatgccgtct gaaacagatg
    19201 gaggactgcg gcgcggacat ctgcaaaatt gcggtgatgc cgcaaagcgc ggaagacgtg
    19261 ctgactttgc tttccgccac gctcgaagcg aaacggcttg ccgccaaacc ggttgttacg
    19321 atgtcgatgg ggcagacggg ggcggtcagc cgccttgccg gacaggtgtt cggctcaagc
    19381 atcacgttcg gttcgggaac gcaaaactcc gcgccggggc aaatcggcgt atccgccctc
    19441 cgtgcgacac tcgactgcct cgaaaacgga gcagactgat ttcagacagc atcaaaacat
    19501 gatgaaactc aatccccaac agctcgaagc cgtccgctac ctcggcggcc cactgctcgt
    19561 ccttgccggt gcaggcagcg gcaaaaccgg cgtgattact caaaaaatca agtatttgat
    19621 tgtcaatgtc ggctatctgc cgcataccgt tgccgcgatt acctttacca ataaagccgc
    19681 cgcggaaatg caggagcgcg tcgccaaaat gctgcacaaa tcgcaaacgc gcgggctgac
    19741 gatttgcacg ttccactctt tgggcatgaa gattctgcgc gaagaggcga accatatcgg
    19801 ttacaaaaaa aacttctcca ttctcgattc taccgacagc gcgaaaatca tcggcgaact
    19861 cttaggcggt acgggcaaag aagccgtatt caaggcgcag caccagattt ccttgtggaa
    19921 aaacgattta aaaacgcctg aagatgtcgt tcagacggca tcgaacattt gggaacaaca
    19981 aaccgcacgc gtgtatgcga gctatcagga aaccttacaa agctatcagg cagtggactt
    20041 cgacgacttg atccgcctgc ctgccgtgct gttgcagcaa aacagcgaag tgcgcaacaa
    20101 atggcagcgg cggctgcgtt atctgctggt tgacgaatgc caagatacga atacctgcca
    20161 atttacgttg atgaaacttc tgaccggcgc ggaaggtatg tttaccgccg tcggcgacga
    20221 cgaccagtcc atctacgcat ggcgcggcgc gaacatggaa aacctgcgca aaatgcagga
    20281 aaactatccg cagatgaagg tcatcaaact ggagcaaaac taccgctcca ccgcgcggat
    20341 tctcaaaatc gccaacaaag tcatcgagaa caaccccaag ctgttcacca aaaaactttg
    20401 gtcgaaattg ggcgaaggcg agcccgtcaa agtcgttgcc tgccaaaacg agcagcacga
    20461 agccgactgg gtcgtcagcc aaatcgtcaa acaaaaactc atcggcggcg acaaaaccca
    20521 atatgccgat ttcgccgtgt tgtaccgcgg aaagcatcag gcgaggattt tcgaggaagc
    20581 attgcgcggc gcgcgcatcc cctaccgtct ctccggcgga caaagctttt tcgacaaagc
    20641 cgaaatcaaa gacgtgttgt cttatgtgcg gctgcttgcc aaccccaatg acgatcccgc
    20701 ctttctgcgt gccgttacca cgcccaaacg cggcatcggc gatgtcacgc tgggcaagct
    20761 caacacttac gcgcacgaac acgaatgcag cctgtatgaa gccgcacaaa acgaagaagc
    20821 ccttgccacg ctgaacaata ccaaccgcca acacctgcaa gcctttatgg atatgttcgg
    20881 cagctaccgc gccaaagcgg aaaccggcga agcaggcgag ttcatcaaca gcctgctcga
    20941 agaaatcgac tatgaaaacc atttgatgca aaacgaagaa ggcaaggccg gcgaaatcaa
    21001 atggcgcaat gtcagcgatt tggtatcatg gtttgcgcga aaaggcgggg aagacggcaa
    21061 aaacatcatc gaactcgccc aaaccgtcgc cttgatgacg cttttggaag gaaaagacga
    21121 agaagaaacc gatgccgtct cgctatccac gctacacgcc gccaaaggtt tggagtatcc
    21181 gtatgttttc cttgtcggtt gcgaagaagg cgttttgccg cacaacgaca gtatcgaaga
    21241 gggcaacgtc gaagaagaac gccgcctgat gtacgtcggc atcacccgcg ccaaacgcca
    21301 actcacgctg acccactgcg tcaaacgcaa aaaacaaggc atatggcagt tccccgaacc
    21361 cagccgattc atagacgaaa tgccgcagga agatttgaaa atcctggggc gcaaaggcgg
    21421 cgaaccgatt gtcagcaagg aagaaggcag gcgcaacctt gccgatataa tcggaaggct
    21481 cgacaaccta aaaaaaagcg gcgcggcgga ttaaaccgga gccgcaatgc cgtctgaagg
    21541 cttcagacgg catatttttt ggacggcgcg cgtaaagcgg tttacgccca caaatcctgc
    21601 tgctggtttt tcggcacaag atgccccacg ccgataccga taaggcggaa cgcgtcttcc
    21661 gtctgcggcg ggacgcgcgc tatcaacatt tgcgcagcct gcagcagagc tgcgcagtcg
    21721 ggcaatacgg aggaataagt cagtgtgcgc gtgatgatgc ggaaatcgta ggtcttcagc
    21781 ttgagcgtta cgctttgggc ttcgacgttt ttgcgcgtga tttgccgcca caggtcttca
    21841 gcaagatggg gcaggtgtcc ggcagcctgc tcgagcggca ggtcttcagg cagggtgatt
    21901 tctgtggaga tttggaggcg ttcgcgttcg gctttgacgg ggcgttcatc cgtaccgcgc
    21961 accaaatcat agaggcggta tccgtagcgt ccgaaatggt ttaagagctc gccgcgctcg
    22021 aaacggcgca agtcgcccgc cgtccgcata cccagcgact gcattttttt cagcgttacc
    22081 ttgcccacgc cggggatttt gcccaaaggc agggtttcca aaaatgccat gactttgtgc
    22141 ggcggcaaca caaactgccc gttcggcttg cgccagtccg acgcgatttt cgccagaaat
    22201 ttgttcggcg cgatgcctgc ggatgcagtc aaacctgttt ccgcaaaaat ggcggcacgg
    22261 atttctttgg caacttcact ggcgtaaggg atgtttttga aattacgggt aacgtcaaga
    22321 taggcttcgt ccagcgacaa gggttcgatt aaatcggtat aacgcctgaa tacggcgtga
    22381 atctgcgcgg aaacctgacg gtacaaatcg aaatgcggcg gcacatacac cgcttgcgga
    22441 cacagccttt tcgccgttgc caccgacatc gcggaatgca gcccgaactg ccgtgcctca
    22501 tacgatgcgg cgcaaatcac cgaacgcgcg ccctcccacg cgacgaccac cggccgccct
    22561 ttcaaatgcg gctgttcgcg cagctctacc gatgcgtaga acgcgtccat gtcgatgtgg
    22621 atgattttgc gtgaagacat cggctctgct gaggataaaa aggatattct actgccggca
    22681 tcgggcaaat tc
//
LOCUS       DAWFHY010000025        22442 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.025, whole genome shotgun sequence.
ACCESSION   DAWFHY010000025 DAWFHY010000000
VERSION     DAWFHY010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22442
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.025"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     repeat_region   375..938
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=388..410
                     /rpt_unit_seq="ttctcatttcgcagtgctacaat"
     gene            complement(1002..1328)
                     /gene="cas2"
                     /locus_tag="WGQ68_000868"
     CDS             complement(1002..1328)
                     /gene="cas2"
                     /locus_tag="WGQ68_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HEZ0714704.1"
                     /translation="MSEAKFMRIIVFFDLPVITAAKRKAANQFRQFLLKDGYQMLQLS
                     VYSRIVKGRDSLQKHHNRLCANLPQEGSIRCLEITEKQYAAMKLLLGELKTQEKKVNS
                     DQLLLF"
     gene            complement(1321..2235)
                     /gene="cas1"
                     /locus_tag="WGQ68_000869"
                     /pseudo
     CDS             complement(1321..2235)
                     /gene="cas1"
                     /locus_tag="WGQ68_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582247.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR-associated endonuclease Cas1"
     gene            complement(2302..5548)
                     /gene="cas9"
                     /locus_tag="WGQ68_000870"
                     /pseudo
     CDS             complement(2302..5548)
                     /gene="cas9"
                     /locus_tag="WGQ68_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742773.1"
                     /note="Cas9, originally named Csn1, is the large,
                     multifunctional signature protein of type II CRISPR/Cas
                     systems. It is well known even to general audiences
                     because its RNA-guided endonuclease activity has made it a
                     popular tool for custom editing of eukaryotic genomes.;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II CRISPR RNA-guided endonuclease Cas9"
     gene            complement(5832..9266)
                     /gene="dnaE"
                     /locus_tag="WGQ68_000871"
     CDS             complement(5832..9266)
                     /gene="dnaE"
                     /locus_tag="WGQ68_000871"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEZ0714705.1"
                     /translation="MTEPTYIPLRLHTEFSITDGMVRIKKLIAKAQEYGLPALGISDL
                     MNEFGLVKFYKACRSAGIKPVGAADVWIGNPNAPDKPFRAMLIIRNDAGYLRLSELLT
                     AAYVGKDRNIHHAELNPEWLANGDNSGLICLSGAHYGEVGVNLLNGNEDAARTAALKY
                     AAWFPDAFYMELQRLPERPEWEACVSGSVKLAEELGLPVVATHPTQFMSRDDFNAHEA
                     RVCIAGGWVLTDKKRPRDFTPSQFFIPPETMLERFADLPEALENTVEIAKRCNLHITL
                     GKNFLPLFPTPDGLSRDDYLIKLSNKGLQERMVQLYPDEAERAAKMPEYQERLDFELN
                     IIIQMKFPGYFLIVQDFINWAKTHGCPVGPGRGSGAGSLVAYSLKITDLDPLKYALLF
                     ERFLNPERVSMPDFDVDFCQANRGRVIEYVRDKYGAQAVSQIVTFGTMSSKAVIRDVG
                     RVLELPFMLCDKLSKLIPLEANKPLGLDDAMKAEPQIQELIEAEEADELITLAKKLED
                     LTRGLGMHAGGVLIAPGKISDYSPVYQADESASPVSMYDKGDVEDVGLVKFDFLGLRN
                     LTIIEMAQNNIKNTTGDIVDVGKIPLDDQAAYQIFRDANTTAVFQFESTGMKKMLKTA
                     HTTKFEELIAFVSLYRPGPMDNIPDFVARMKGQEFQYIHPLLEGILAPTYGIMVYQEQ
                     VMQAAQIIGGYSLGGADLLRRAMGKKKPEEMVKHREIFAEGAAKQGISREKSDEIFNY
                     MEKFAGYGFNKSHAAAYALISYQTAWLKAHYPAEFMAATMSSELDNTDQLKHFYDDCR
                     ANGIEFLPPDINESDYRFTPYPDMKIRYALGAIKGTGEAAVESITAARQSGGKFTGLL
                     DFCERVGKEHMNRRTLEALIRGGAFDSIEPNRAMLLANIDLAMNNADQKAANANQGGL
                     FDMMEDAIEPVRLIDAPMWSESEKLAEEKTVIGFYLSGHPFGPYAQEVRQIAPQKLSK
                     LKPQDSVRLAGFVTAVRTMMGKRGKIAFVSLEDLSGQVEIMVGGQTLENCADCLKADQ
                     VLIIESKVSRDDYGGGDGLRIMANQVMTLQTARERYARSLSLALAPHHDIGGLVRLLS
                     SHQLPDTPRIPLQLSYANEKASGRLQVPPKWTVTPSSALFGELETLLGSRSVRVNW"
     gene            9804..10037
                     /locus_tag="WGQ68_000872"
     CDS             9804..10037
                     /locus_tag="WGQ68_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEZ0714706.1"
                     /translation="MEATVYLEDNEYIALCDLLKLAGLAESGGQAKALIAEGLVSRNG
                     ETEIRKTAKIRGGEVIEFDGARLEIADGYDPEA"
     gene            10015..10224
                     /locus_tag="WGQ68_000873"
     CDS             10015..10224
                     /locus_tag="WGQ68_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714707.1"
                     /translation="MDTTLKHKAEALLGEPLLEEPVRPELWECCGSDCGEACIQTIYW
                     ADKARYDAQRRKLKEAGWAEDENAV"
     gene            complement(10266..10508)
                     /locus_tag="WGQ68_000874"
     CDS             complement(10266..10508)
                     /locus_tag="WGQ68_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714708.1"
                     /translation="MCVVNGGQGVSILSGGVAAVISKNKCMANAKEKPLCRHSRAGGN
                     PDFDLLEMFKVNRYFKLPDSCFRGNGDMTVFNFNLL"
     gene            10517..11353
                     /locus_tag="WGQ68_000875"
     CDS             10517..11353
                     /locus_tag="WGQ68_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEZ0714709.1"
                     /translation="MIDLHCHSTVSDGMLSPAEIVRLAHQNGCTLLALTDHDHTGGIA
                     EARAEADRLGLRLINGVEISVTWRGRTIHVVGLDFDEQDENLQNLLAQVRQGRLKRLE
                     AIADKLEKKGIGGAYDGALELAANKEMVSRTHVAEFLIKNGYVKNKQQAFTKYLGDGK
                     SCAVRHEWATLEDCVSAVNGAGGLAVIAHPMRYDLSATAKRNLFEEFKNLGGAGIEVH
                     SGNCCKNDRLNYALLAERFGMLASAGSDFHRLNDFSGGILGACPELPENCRPVWAHFS
                     RH"
     gene            complement(11406..12698)
                     /locus_tag="WGQ68_000876"
     CDS             complement(11406..12698)
                     /locus_tag="WGQ68_000876"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HEZ0714710.1"
                     /translation="MQFSAFGEKFTQHSGILQLMDDLGDALKSDKPVNMLGGGNPARI
                     AEVNAVFADVFSKLAAEHAVENIGNYSNPQGDAALIDALTAFLNREYGWNLTADNIAL
                     TNGSQNAFFYLFNLFGGKFNLSDGTSAEKAILLPLAPEYIGYADVHVEGQHFVSVKPK
                     IENVEHEGEAGFFKYRVDFDALENLPELKAGKIGAICCSRPTNPTGNVLTDGEMARLD
                     ALAREHGIPLIIDNAYGMPFPNIIYSDVTLNWHENIILCFSLSKVGLPGVRTGIIVAA
                     PEVVKAVSSLNAIVNLAPTRFGAAIAAPLLESGEMKRLADQVIRPFYRNQAQTAVSLL
                     KRELGAYPMKIHKPEGAIFLWLWFENLPVSSQTLYEMLKAEGTLIIPGEHFFVGIDTQ
                     DYPHAGECIRMSIAQDAQTLEKGIAAIGKTVRKLYDNV"
     gene            complement(12756..14666)
                     /gene="pglD"
                     /locus_tag="WGQ68_000877"
     CDS             complement(12756..14666)
                     /gene="pglD"
                     /locus_tag="WGQ68_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent dehydratase PglD"
                     /protein_id="HEZ0714711.1"
                     /translation="MTLETLIALPRNIKKICFLIHDFLMIFIAFWFTQSLKADYSNEW
                     FDPANWQSFLLTALLTIGLFVRMGLYRAVTRFVSFHVLTTAFAGSIVSAVLFFLNTLI
                     FEERLRLALPIVYFLLLFVSVTGSRMVLRGLLSEHPKKQMIPVIIYGAGRSGRQLLEA
                     VKQMREYSAAAFVDDDPKLWHTVIYDLAVYQPDAIAFLIERYGVEKILLAIPGATQEQ
                     RRRIINKLEAYPCEVLTIPGMKDLMDGKISIGTLKKISVSDLLGRDSVAPDDRLMSAD
                     IEGKTVMVTGAGGSIGSELCRQIIRRRPEKLLLFELSEFALYAIEKELRETCIQKRLD
                     TEILPFLGSVQNRTLLEHVMTAFSVATVYHAAAYKHVPMVEFNTVEGIRNNIFGTLEC
                     ALAATTSGVRTFVLISTDKAVRPTNTMGASKRMAELCLQALAAEPGQKTRFSMVRFGN
                     VLGSSGSVVPLFEKQIAEGGPLTLTHPEITRYFMTIPEAAQLVIQAGAMGTGGDVFVL
                     DMGESVKIIDLARQMITLSGLKPNTPEQPDGDIEILITGLRPGEKLYEELLIGDNVRK
                     TAHPRIMTADEAMLPWHELSVLLDRIRSACDRYDQQAIRTLLINAPTGFTPSDGICDL
                     LWVRETHRKNAV"
     gene            complement(14714..15889)
                     /locus_tag="WGQ68_000878"
     CDS             complement(14714..15889)
                     /locus_tag="WGQ68_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEZ0714712.1"
                     /translation="MLNTALSPWPCFTQEEADAVSKVLLSNKVNYWTGNECREFEKEF
                     AAFAGTRYAVALSNGTLALDVALKAIGIGAGNDVIVTSRTFLASASCIVNAGANPVFA
                     DVDLNSQNISAETVKAALTPNTKAIIVVHLAGMPAKMDGIMALAKEHDLWVIEDCAQA
                     HGAKYKGKSVGSIGHVGAWSFCQDKIMTTGGEGGMVTTNDKELWSKMWSYKDHGKSYD
                     AVYHREHAPGFRWLHERFGTNWRMMEMQAVIGRIQLKRLPEWTVRRQENAAKLAESLG
                     KFKSIRLIEVADYIEHAQYKFYAFVKPEHLKDGWTRDRIVNELNVRKVPCYQGSCSEV
                     YLEKAFDNTPWRPKERLKNAVELGNTSLMFLVHPTLTDDEIAFCKKHIEAVLAEATQ"
     gene            complement(16008..16124)
                     /locus_tag="WGQ68_000879"
                     /pseudo
     CDS             complement(16008..16124)
                     /locus_tag="WGQ68_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(16360..17046)
                     /locus_tag="WGQ68_000880"
     CDS             complement(16360..17046)
                     /locus_tag="WGQ68_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0714713.1"
                     /translation="MKTINPETTVIVFDLDDTLYSEYEYKLSGIRSVIDTVAALYPDW
                     NSDNLWRNIDPDGKDWLDKLCRHCGFNESEKQVLLWQYRLHRPTLTPYAPPDFLSELT
                     APFAARALITDGRSLTQRLKLEALGLSSLFDDILISEACSSEKPDGKRFRHLQDKYAD
                     KAGCFIYIGDNISKDFIAPNTLGWITIGLLPSGQNIHRHQPESFNTEYHPDFWVNSLQ
                     DLVPIIDSNT"
     gene            complement(17033..18610)
                     /locus_tag="WGQ68_000881"
     CDS             complement(17033..18610)
                     /locus_tag="WGQ68_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEZ0714714.1"
                     /translation="MSKFFKRLFDIAASASGLIFLSPVFLILIYLIRKNLGSPVFFFQ
                     ERPGKDGKPFKMVKFRSMRDALDSDGIPLPDGERLTPFGKKLRAASLDELPELWNVLK
                     GDMSLVGPRPLLMQYLPLYDNFQNRRHEMKPGITGWAQVNGRNALSWDEKFACDVWYT
                     DNFSFWLDMKILFLTVKKVLIKEGISAEGEATMPYFTGNQTNEKNNILILSAGRRVEL
                     VQDFQTEAARFSDGISVFATDLNPRMSSACHVSDGSFEVPPISADSYIDSIFNLAVRE
                     NIGLIIPTIDTELQKLADERERFEAASVHIVVSDSDFIAQCRDKRKTATLFARYGIRS
                     PEIYDRKKLVFPCFAKPYDGSRAIGAKRIDTPADLTPEVLADTKLMFCQFIDIENKFS
                     EFTVDMYYDRQCRLKCAIPRKRLEVRSGEISKGVTCKNSLYQTLLEKMSVLQGARGCI
                     TVQFFYNEETGEFYGVEINPRFGGGFPLTYAAGGNYPSRLMREYLGDEEIPFSDDWEN
                     NLIMLRYDAKVLVHEND"
     gene            complement(18603..19769)
                     /gene="pglH"
                     /locus_tag="WGQ68_000882"
     CDS             complement(18603..19769)
                     /gene="pglH"
                     /locus_tag="WGQ68_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminyl-a1,3-N,
                     N'-diacetylbacillosaminyl-a1-diphospho-di-trans,
                     octa-cis-undecaprenol synthase PglH"
                     /protein_id="HEZ0714715.1"
                     /translation="MNITIVAPYCSLPSEPYFNRFWYLAERLSQSHDVLLITSRFRHY
                     DKSFRRHEDAAATSNGRLRVKLLDEPGYRKNVSLARVASHRVFVRNLARWLHSPQAAE
                     QDIVYSAYPLMATNLLLGKHKARLGYKLIVDVQDVWPESFSSVVPFLKKVPHKLLPFA
                     SRANRAYRCADALVAVSQTYLDRAKEANPNVPGEAVYIGTDFAAIAPPPRFRSKTTRL
                     FYLGTLSYSYDVETVCKGVRKLLDDGENVELHIMGGGPDLEKLKQHENRAIKFYGYLP
                     YADMISIAKGCDIAVNAIHSHAMQSVTNKLSDYMALQKPILNSQTNAEVLGLLNLLPH
                     ENYRSGDVDSFVQAAKDILKRKNDPVQSDEIVRRFRRDISYRKIVNLIERLAHE"
     gene            complement(19789..20313)
                     /locus_tag="WGQ68_000883"
     CDS             complement(19789..20313)
                     /locus_tag="WGQ68_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEZ0714716.1"
                     /translation="MSRLDTIRAVHLMLDIFKKMVDRDMPVRLNVAGIGEEMDNLKAQ
                     AERLGIDGKVTFLGGVRDLTAYFKDVDILVNTPHCIGDHGAGVGNNILEAGLYDTPVV
                     TYDMAGISEMVVNGETGYCIPFGDEEAFIAAVDTLVRQPLLREAMGKRLHAHVAALCS
                     DDEIYRTTMAAYTM"
     gene            complement(20252..20860)
                     /locus_tag="WGQ68_000884"
                     /pseudo
     CDS             complement(20252..20860)
                     /locus_tag="WGQ68_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243816.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(20857..22278)
                     /locus_tag="WGQ68_000885"
     CDS             complement(20857..22278)
                     /locus_tag="WGQ68_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEZ0714717.1"
                     /translation="MDTKEILGYAAGSIGSAVLAVIILPLLSWYFPADDIGRIVLMQT
                     AAGLTVSVLCLGLDQAYVREYYAAADKDTLFKTLFLPPLLSAAAIAALLLSRPSLPSE
                     ILFSLDDAAAGIGLVLFELSFLPIRFLLLVLRMEGRALAFSSAQLVPKLAILLLLPLT
                     VGLLHFPANTAVLTAVYALANLAAAAFLLFQNRCRLKAVRRAPFSSAVLHRGLRYGIP
                     IALSSIAYWGLASADRLFLKKYAGLEQLGVYSMGISFGGAALLFQSIFSTVWTPYIFR
                     AIEANTPPARLSATAESAAALLASALCLTGIFSPLASLLLPENYAAVRFIVVSCMLPP
                     LFCTLVEISGIGLNVVRKTRPIALATLGALAANLLLLGLAVPSGGARGAAVACAASFW
                     LFFVFKTESSCRLWQPLKRLPLYMHTLFCLASSAAYTCFGTPANYPLFAGVWAAYLAG
                     CILRHRKDLHKLFHYLKKQGFPL"
BASE COUNT         4715 a         5519 c         6515 g         5693 t
ORIGIN      
        1 tttgcaagat tttagtttaa tcatcaaaca gggaacagca aaaatccccg ctgcaacggg
       61 gatttttgtt cggctctcgc ctgtgtgatt aacttcacta ggagaagcta aaatgcttaa
      121 attccttatg cgtgggaaaa agttgctgct gtccgttgaa gtgaaccaca agttcatttt
      181 agcggttgct ttgctgttaa gtcaatgcag tagctaaccc gaccacaatc aaaaaaacgc
      241 aagttttccg cctactctgt gagtgggcgg ttttttctta tttcaaatct gtgacacaat
      301 atctgtcgcc tttcttgcca taattgcact gtgtctcaat gactttaacg cacgttcgct
      361 tatcgcaacg gctggttgta gcgcccattc tcatttcgca gtgctacaat gcggaacggg
      421 ttgacagact gaatttgagt gttgtagctc ccattctcat ttcgcagtgc tacaatcgac
      481 aacaaagccg tctatgacct gaccgagttg tagctccctt tctcatttcg cagtgctaca
      541 atatgcgcgt aaacacttta cgcaaggcgt tggttgtagc tccctttctc atttcgcagt
      601 gctacaatct tctgcctttt tacaagctcg ctttctttgt tgtagctccc tttctcattt
      661 cgcagtgcta caatgccacg cacctacacg gggtggtttg ttaagttgta gctccctttc
      721 tcatttcgca gtgctacaat tcgcacacgc aatggagctt atcgtttact gttgtagctc
      781 cctttctcct ttcgcagtgc tacaatttca ttccgattgc cttgcgttca aggtgtgttg
      841 tagctccctt tctcctttcg cagtgctaca attgcaatct gattttccgc cgtctataca
      901 tcgttgtagc tccctttctc ctttcgcagt gctacaatac ttcgacggga aatccttatt
      961 tcataagggt ttcccgtttt atttgttatg aaaaaatggg cttaaaataa caataattgg
     1021 tctgaattga cctttttttc ttgggttttc agctcgccca acagcagttt catggcggca
     1081 tattgctttt ctgttatctc caaacagcgg attgagcctt cttgcggaag gtttgcacat
     1141 agtctattgt ggtgtttttg caacgaatcg cggcctttga cgatacggct gtatacggaa
     1201 agttgcagca tttggtatcc gtcttttaat aaaaactggc ggaattgatt ggcggctttg
     1261 cgctttgccg ccgtgataac cggtaggtcg aagaagacga taatcctcat aaatttggcc
     1321 tcactcatat tggtatgctt tcaatggtag gatttcaggc agtttcagtt gtttggcgtt
     1381 tttatcagtt acgccggctt gaaatgacga aaccattttg tcgatggcag ccaaggtgct
     1441 gaaatgttgc cgttcgatgc tggtttgata gtagaggatt ttgatgaggt gttgtttgat
     1501 gccgagcgtc agttcagttt tcagacggcc ttgttcgtac aaatgtatca cggtcaggtc
     1561 ggccaatggg cgtaagggtt cgataaagtc atcggcgaga ttaaatggat tgagttcact
     1621 tctatgaaac aagcctaatg caggtaacca accgtataag gtcagggcgc gtgcaactgc
     1681 ggctcggagc acggcgtagg tgtagttcaa tgcggcattg acggcgttgt tgtcattacg
     1741 ggtaaatttt tcgccaaaga gggcttggaa gtagagggcg gcggcttacg cttcgcggtt
     1801 tcctgtatcg ccagagcgga cttcagatgc caaagtgcgc agccgtttgg ctgcaagatc
     1861 attgccggtt tcgtctgcga caaaggcttg attcaggatt ttttggcgga cgatatgttg
     1921 ccagaattgc ttttttaagg gttcgcttat attcagctgc agtttgagga ttttaagttg
     1981 gcggtgatat tgggcataag gcagccattg tccgcagggc aggaactgct cgtcacatgt
     2041 cagtaaggtt gcgccatgtt cggctaaggc tgacagaagt ggagcggtaa tcagggtttc
     2101 gcggttttcg atgatgataa cggcgatgtc ctctaatggt acggtgtggg actcgccgtt
     2161 ttgttgaatc agcagttgcc ggcgttgcaa ggatagtttg ccgccgtttt gaataagcag
     2221 gctgcgccaa gtcatgtttt tccccttatt tttatataaa aaatccctgc ttcaggccgc
     2281 ctgaaacagg gatagattaa tttaacggac aggcgggcgt tttttcagac ggcatggtct
     2341 gatttctttg cccagttcgt caatttggta tttttggaat gaaagggcgg ttttgacgcc
     2401 gataccttcc agtattccat ttttgccaat tttatgatca agatcatgaa tgcgtatatt
     2461 gatattacct gtgcctcgat ggcagctggc aaagtaacca aacattctag ctttttttgt
     2521 tataacctcg actaaatcat tagggtgtaa tgagaattta aagttgaaac tatcatcaat
     2581 aagttgccaa tcttcttcat cttttccttg tacaacagcc ctatccggca aaatcccttt
     2641 cgctacctgc caactgtaaa tcggtaccag ataatacttg tcgcctttct caaacacatc
     2701 tacgcgcacc atggttgcgt tgtcggcaat accgttatgg ttgcgcaccc atacgccggt
     2761 tttctgtact tgctctacgc gtacggcttt tacctgttgg gtgcggttgc ctgctttatc
     2821 gtatttgtaa aacggctcgg caaaggcttt ggcaggatcg tctttatgtg cttccagccg
     2881 tgctttcagt gcttcgtata gcttaggttc gcgctcccga ttgaccattt tttccaagtc
     2941 tttcagtttt aactgtgtta acggtacgcg caacacgctg acgccttcgt ccagtcgttt
     3001 ggcggatttg acggtctcca tatgcccttg cccgctcatc ttccgattgg gcgcgcgtga
     3061 aacaaacagt ggcgtaacgt attcgtgtac ggcttcgggg cgagatgata atttttcggc
     3121 aagcaacgtg cgcagttttt ctggggtatc ggcttcttcg aattcgggtt tgccgtccgg
     3181 tttgccgaag acgcgaatca tgacttcttg tgcgaaaaat tcccaaggtt gtgggaagtg
     3241 tgttttttga tgcagcactt ctcctgtttc tttgtctatg gttttaccgt caaacgcgtt
     3301 catctcttta tagcgtacaa aacgggtaat tttctgctgc atggcaacgg tcgagcaggc
     3361 aacgacgacg gcgtccaagg cgtgatggcg gtcgttttcc gcacgcactt tgcgtaatcc
     3421 ccaaaagccg cgcaacagat tggtaatttg tccgtttgag gcaaagacac gtcttttccc
     3481 tttacctgtc agcagcatat ggtcggcaac gaattggcac aggaaacggt tgacgtagcg
     3541 cgtatcgttc agattgcgtt ctttaaagcc ctcttcatcg aatttttgca gcagaatccg
     3601 ttgtttttta ctgcgtggga aacggctggt ttcgacacgc gctttaaatt cctgccattc
     3661 gcggctgttg tctttgccgt tgaagtattc gtaaggggtt tgattgcctt tgttttggtt
     3721 ttcgctgccc aataccagca ctttattgtt gaaactgtcg tcccatgtgc gcgagaacgg
     3781 cagggcatgg tcgatttcga catagccttt ttcgttcaga cggcctaagt tgatttcttt
     3841 gcccgaatac aggcatttgc cgtgttgttg ctcgtacagg cgcagtttca gaatatcttt
     3901 ggatttgggt tcgccgacaa aattggggaa atactctcgg aatttggtgg cggctttttc
     3961 acggtcttta cggttttctt cttggcgttt ttcgatttct ttgcggtctt taaacgattt
     4021 tcctacttcc cttgccgttt caatatggat acgcgcaggt gagtcgtaac ggcgcacaac
     4081 gccgttaatg acttttctgg cctgagataa ggcgcgtaag acgacggggt tgcggatttc
     4141 gtcggcaggg atgggtggca gataaatctt ttcttccgta ttcttcttgc cgtcatggtc
     4201 gccgtagatt tcggcgcagg cttcatcgta acgtttgcct tgttccatta gaggcacaat
     4261 tcggcgcaat gctttcaagg aaatttgaac gaacttgtcg aagctgatgt gttttaacag
     4321 tgcttctaag atttcgggct gtacgcggtc tttcagacgg cctgtaatgt cttcatcggt
     4381 tttgaacagg gagaatgccg tgccgatttc gtcttgtaat tcgggagaaa ggtttaatgg
     4441 ggattttttg tctttcaatc cttctttttc cagcgcacgg ctgatggcat ggtaggcctt
     4501 catttccatc aacgttgagg cttcggcatt gtctttacca tagcgcaagc ctttgaaaaa
     4561 ggcggtatcg tctagctcca aaagcttacg ggcttgtgcg taagtcagtt tggattttct
     4621 gtatggctcg tccataagcg tggcgcgttg ggtatcggtc aatggccgct cgctgccttg
     4681 ctctaaaata cgcaggttgt tcagcttggt cagccagacg aaacgttcgg cggtataggt
     4741 gtttttagcg gctttcggct ctgccgactc aaaggtgcaa tgccccaaca ttttttgaac
     4801 agcatcgccg gacagggcag ggcgttgcgt catcagcagg gtttcaatac cttctttaag
     4861 gccgtctgaa atatgcggat tgccgaattc tttttgtttt tcaaacaata aattcagctc
     4921 cgcctgcaaa tctttgcggc tgaacgtatg cgaataatcg ccgcgctggt tgcggatatg
     4981 gccgctttct ttctcaaatt tatttaaagc caattcggcc ggtgtgcgga aatcgcctgt
     5041 ctgtaaggca tgggcattgc cggctacgcc tttaagcaaa gcgccaagct ccttatcggc
     5101 agtttcgccc tcgtttttcc gttgcgataa atagccgcga tgtttgatta aatgcaacaa
     5161 gactgccgac cactccaaag gcgtcagttt gcggtctaat gcggctgagc gaagttgcca
     5221 tggtgtattc ggtaaggatt taatcaagcc gttttcatca aaatcggcgg cttgtaatac
     5281 gccttcgcgt tttaataggc gacgagctcg aagcaggcgg tgggcgcgac ggcgggtcag
     5341 gcggcacgct gcgtgccaag cgtcttgctg ctgcgagaga gtcacctgtt ttcggtactt
     5401 ctgcgcgctc aaatacgcgc acgcctaaat caatcaggca gattgggttt tcatcttcgt
     5461 caatttctac catcgcccag ccgacggatg cgatgccgat atcgaggccg aggatgtagt
     5521 tgattggatt aggtttgaag gcagccattt attactccgt aaacaacgtt gcaaataaaa
     5581 ataatgataa tatatcttat tgtcttattt atatacaaat agattattga cttatcatct
     5641 cacaacggct agaatcccaa acatattgtc gcactgcgaa atgagaaccg ttgctacaat
     5701 aaggccgtct gaaaagatgt gccgcaacgc tctgcccctt aaagcttctg ctttaagggg
     5761 catcgtttat ttcggctaaa aaatgccgtc tgaaaccgat tttgggcttc agacggcatt
     5821 tatattttgg gttaccagtt gacgcgcacc gaccggctgc cgagcaatgt ttccagttcg
     5881 ccgaacaatg cggagctcgg tgtaaccgtc catttcggcg gcacttgaag cctgcccgac
     5941 gctttttcgt tggcatacga cagttgcagc gggatgcgcg gcgtgtcggg cagttggtgg
     6001 gaggagagca gccgtaccag tccgccgatg tcgtgatgcg gggcgagggc gaggctgagg
     6061 ctgcgggcgt agcgttcgcg tgccgtttgc agggtcatga cttggtttgc catgatgcgc
     6121 agtccgtcgc cgccgccgta gtcgtcgcgg ctgactttgg attcgataat cagcacttgg
     6181 tcggctttga ggcagtcggc gcagttttcc aacgtctgac cgccgaccat gatttcaacc
     6241 tgcccgctca aatcttcaag gctgacgaag gcgattttgc cgcgcttgcc catcatggtg
     6301 cgtacggcgg taacgaatcc ggcgaggcgc acgctgtctt gcggtttgag tttcgacagt
     6361 ttctgcgggg cgatttggcg gacttcttgg gcatacgggc cgaacgggtg gccggacagg
     6421 taaaagccga tgacggtttt ttcttcggcg agtttttccg attcgctcca catcggcgcg
     6481 tcaatgagcc gcaccggttc gatggcgtct tccatcatgt cgaaaagccc gccctgattg
     6541 gcgttggcgg ctttttggtc ggcgttgttc atggcgaggt cgatgtttgc caagagcatg
     6601 gcgcggttgg gttcgatgct gtcgaacgcg ccgccgcgta tcagggcttc gagggtgcgg
     6661 cggttcatat gttctttgcc gacgcgctcg cagaagtcca agaggccggt aaacttgccg
     6721 ccgctttgcc gcgcggcggt gatggattcg acggcggctt cgcccgtgcc tttaatcgcg
     6781 ccgagcgcgt agcggatttt catgtccgga tacggcgtga agcggtagtc ggattcgttg
     6841 atgtcgggcg gcaggaactc aatgccgttg gcgcggcagt cgtcgtagaa atgcttgagc
     6901 tggtcggtgt tgtccaattc ggacgacatg gtcgccgcca taaattcggc ggggtagtgc
     6961 gccttgagcc atgcggtctg gtaggaaatc agggcgtagg cggcggcgtg ggatttgttg
     7021 aaaccgtagc cggcgaattt ttccatgtag ttgaagattt cgtcggattt ttcgcgcgaa
     7081 atgccttgtt ttgccgcgcc ttcggcaaag atttcgcggt gtttcaccat ttcttcgggc
     7141 tttttcttac ccatcgcgcg gcgcagcagg tccgcgccgc cgagcgagta gccgccgata
     7201 atttgcgccg cctgcatcac ttgttcctga tacaccataa tcccgtaggt cggcgcgagg
     7261 atgccttcga gcagcgggtg gatgtattgg aattcctgac ccttcatgcg cgcgacgaag
     7321 tcggggatgt tgtccatcgg accgggtcgg tagagcgata cgaaggcgat gagttcttca
     7381 aacttggtcg tgtgcgccgt tttcagcatt tttttcatgc cggtcgactc gaactggaag
     7441 acggcggtgg tgttcgcatc gcggaagatt tggtaggcgg cctggtcgtc aagcggaatc
     7501 ttgccgacat cgacaatatc gccggtggtg tttttgatgt tgttctgcgc catttcgata
     7561 atggtcaggt ttcgcagacc caaaaagtcg aatttcacca agcccacgtc ttccacgtcg
     7621 cctttgtcgt acatcgatac tggcgaggcg gattcgtctg cctgatacac ggggctgtaa
     7681 tcggaaatct tgcccggcgc aatcaatacg ccgcccgcgt gcatacccaa gccgcgcgtc
     7741 aggtcttcca gctttttcgc cagcgtaatc agttcgtccg cttcttccgc ttcgattaat
     7801 tcctgaatct gcggctccgc cttcatcgcg tcgtccaaac ccagaggttt gttggcttcc
     7861 aacggaatca gcttggacag tttgtcgcac agcataaacg gcagctccaa cacgcgcccc
     7921 acgtcgcgga tgaccgcttt ggacgacatc gtgccgaagg taacaatctg gctgaccgcc
     7981 tgagcgccgt atttatcgcg cacatattcg atcacgcggc cgcggtttgc ctgacagaag
     8041 tcaacgtcga agtcgggcat ggagacgcgt tcggggttta agaaacgttc aaacagcagc
     8101 gcgtatttca gcgggtcgag gtcggtaatt ttcagcgaat acgccaccag cgaacccgcg
     8161 cccgaaccgc gtcccggccc gaccggacag ccgtgcgttt tcgcccagtt gataaagtct
     8221 tgtacgataa ggaaatagcc ggggaatttc atctggatga tgatgttcag ctcaaaatcc
     8281 aaacgttcct gatattccgg catttttgcc gcacgctccg cctcgtcggg ataaagctga
     8341 accatgcgtt cctgcaagcc cttgtttgag agtttgatga gatagtcatc gcgcgataaa
     8401 ccgtcgggcg tggggaaaag gggcaggaag tttttgccca atgtgatgtg caggttgcag
     8461 cgtttggcga tttccaccgt gttttccaag gcctcgggca aatcggcgaa acgctccagc
     8521 atcgtttccg gcggaatgaa aaactggctc ggcgtaaaat cgcgcggacg ttttttgtcc
     8581 gtcaataccc agccgcccgc gatgcacacc cgcgcctcgt gtgcgttgaa atcgtcgcgg
     8641 ctcataaact gtgttggatg cgtcgctacc accggcaaac ccagttcctc cgccagcttc
     8701 acgctgcccg aaacgcaagc ctcccattcg gggcgttcgg gtaggcgttg cagctccata
     8761 tagaacgcat cggggaacca cgccgcatac ttcaacgccg ccgtacgcgc cgcgtcttca
     8821 ttgccgttca acagattcac gcccacttcg ccgtaatgcg cgccgctcaa acaaatcaag
     8881 ccgctgttgt cgccgtttgc cagccattcg ggattgagtt ccgcatgatg gatattgcgg
     8941 tctttgccga cataggccgc cgttaaaagc tcgctcaagc gcagatagcc cgcatcgttg
     9001 cggataatca gcatggcgcg aaacggcttg tcgggcgcat tcggattgcc tatccatacg
     9061 tccgccgcgc cgacgggctt aatccccgcg ctgcggcagg ctttatagaa tttcaccaag
     9121 ccgaattcgt tcatcaaatc gctgatgccc aaagcaggca aaccgtattc ctgcgctttg
     9181 gcaatcagtt ttttaatccg caccataccg tcggtaattg agaactcggt atgcaggcgc
     9241 aaggggatgt aggtcggctc ggtcatggca aaatcggcgt gaacaataaa aggcgtattg
     9301 tagcagggtt gtctttagat ggcggtgtag gtcatgccgt ttcagattca gacggcatga
     9361 cctgcaaatg tttttgagct tttacgacgg caaaaaaatg cctcctaccg tgtggtggag
     9421 gcttcccaag gagtattgat agatataaag gactatcaaa ctagttataa ggaactatat
     9481 accttattcg gacggatggc aagcagttaa attaatttta cgttcaaaca ggtttttgat
     9541 ttcgttttga tgccgattgc cggtgtatcg ggcagttcgc gtttgaggat gtgcatcagc
     9601 gtcaatgcgg attcgtcggg gaacaggatt tgcagtctgc cgttgggcag gatttctttg
     9661 aacggattgc ggcatatggg gtttcagacg gcatttttac ttttacggaa caggcagtgc
     9721 ggatacatcg ctggttttgc ggtaattttg ggtacggcgg cggcaggtgc tacaataacg
     9781 gcctctttct gcaagggaac attatggaag ccaccgtcta tctcgaagac aacgaataca
     9841 tcgccctgtg cgacctcttg aaattggccg gacttgccga aagcggcgga caggcgaagg
     9901 cgttgattgc cgaagggttg gtgtcgcgca acggcgaaac cgaaatccgc aaaaccgcca
     9961 aaatacgcgg cggcgaagtc atcgagtttg acggcgcgcg cttggaaatc gccgatggat
    10021 acgaccctga agcataaagc cgaagccctt ttgggcgagc cgcttttaga agaacccgtc
    10081 cgccctgagt tgtgggaatg ctgcggcagc gactgcggcg aggcgtgcat tcagacgatt
    10141 tactgggcgg ataaggcacg ctacgacgcg caacggcgga agttgaaaga agcgggttgg
    10201 gcggaagacg aaaatgccgt ctgaaaccag ttcggcttga taaaaccacg ttttcagaca
    10261 gtcttttata gtagattaaa attgaaaacg gtcatatcgc cattccctcg aaagcaggaa
    10321 tccggaagtt tgaaatagcg gttaacctta aacatttcca ataaatcaaa gtctggattc
    10381 ccgcctgcgc gggaatgacg gcatagcggc ttttcctttg catttgccat acacttgttt
    10441 tttgaaatca ccgccgccac accgcccgac aatattgaaa caccctgccc accatttacc
    10501 acgcacataa cgccttatga tagacctgca ctgccattcc accgtttccg acggtatgct
    10561 ctcccccgcc gaaatcgtgc gcctcgcgca tcaaaatggc tgcacgctgc tggcgttgac
    10621 cgaccacgac cacacgggcg gcatcgccga agcgcgtgcc gaagccgaca ggctcggttt
    10681 gcgcctgatt aacggcgtgg aaatctccgt aacgtggcgc gggcgtacca tacacgttgt
    10741 cggtttggat ttcgacgagc aggacgagaa cctgcaaaac ttgttggcgc aagtgcggca
    10801 aggccgtctg aaacgtcttg aagccatcgc cgacaagctc gaaaagaaag gcatcggcgg
    10861 cgcatacgac ggcgcgctgg agttggcggc aaacaaagaa atggtcagcc gcacccacgt
    10921 cgccgagttc ctcatcaaaa acggatacgt caaaaacaag cagcaggcgt tcaccaaata
    10981 cttgggcgac ggcaaatcct gcgccgtccg acacgaatgg gcgacgctgg aagactgcgt
    11041 ctccgccgtc aacggcgcgg gtgggttggc ggtgattgcc catccgatgc gctacgattt
    11101 gtccgccacc gccaagcgca atctgtttga agaatttaaa aacctcggcg gcgcaggcat
    11161 cgaagtccac agcggcaact gctgcaaaaa cgaccgcctc aactacgcgc ttttggcaga
    11221 acgcttcggt atgctcgcca gcgcgggcag cgacttccac cgtttaaacg acttcagcgg
    11281 cggcatcctc ggcgcgtgtc ccgagctgcc ggaaaactgc aggccggtgt gggcgcattt
    11341 ttcccgacat tgaatgcgga taaaaatgcc gtctgaaaac ctttcagacg gcattttttt
    11401 gcgttttaaa cgttgtcgta cagttttcgg acggttttac cgatggcggc aatgcctttt
    11461 tccagcgttt gagcgtcctg cgcgatactc atgcggatgc actcgcccgc gtgcgggtaa
    11521 tcctgcgtgt cgatgccgac gaagaaatgt tcgcccggaa taatcagcgt gccttcggct
    11581 ttgagcattt cgtacagggt ttgcgaagaa acgggcaggt tttcaaacca gagccacagg
    11641 aaaatcgcgc cttcgggttt gtggattttc atcgggtacg cgcccagctc gcgcttgagc
    11701 agcgagacgg cggtttgcgc ctgattgcgg taaaacggcc ggatgacttg gtcggcaagc
    11761 cgtttcatct cgccgctttc cagtagcggg gcggcgatgg ccgcgccgaa gcgcgtgggg
    11821 gcaaggttca caatcgcgtt caggctgctg acggctttga ccacttccgg cgcggcgacg
    11881 atgatgccgg tgcgcacgcc cggcaggccg actttggaaa ggctgaagca gaggatgatg
    11941 ttttcgtgcc aattcagcgt cacgtcgctg taaatgatgt tggggaacgg cattccgtag
    12001 gcgttgtcga taatcagcgg aatcccgtgt tcacgcgcca aagcgtccaa acgcgccatt
    12061 tcgccgtcgg tcagcacgtt gccggtcggg ttggtcgggc gcgaacagca aatcgcgccg
    12121 attttgcccg ctttgagttc gggcaggttt tccagtgcgt caaagtccac gcggtatttg
    12181 aagaagcctg cttcgccttc gtgttcgacg ttttcgattt tgggcttgac ggaaacgaag
    12241 tgctgccctt cgacatgcac gtcggcatag ccgatgtatt cgggcgcgag cggcaacaaa
    12301 atggcttttt ctgcggatgt gccgtctgaa aggttgaatt tgccgccgaa gaggttgaat
    12361 aaatagaaaa acgcgttttg cgaaccgttg gtcagcgcga tattgtcggc ggtcaggttc
    12421 cagccgtatt cgcggttgag gaaggcggtc agcgcgtcaa tcagcgcggc atctccttga
    12481 ggattggagt agttgccgat gttttcgacg gcatgttctg ctgccagttt ggaaaatacg
    12541 tcggcgaaca cggcattgac ttcggcgatt cgcgccggat tgccgccgcc gagcatattg
    12601 acgggcttgt cgcttttgag cgcgtcgccg aggtcgtcca tcagttggag gatgccgctg
    12661 tgttgcgtga atttttcgcc gaatgccgaa aactgcatat caaactccgt gtgtgtgtca
    12721 ggcaggttgg tttgtacgtt atgccgtctg aaggttcaga cggcattttt tctgtgtgtt
    12781 tctcgtaccc aaagcaggtc gcagatgccg tcgctcgggg taaagccggt cggggcgttg
    12841 atgagcaggg tgcggattgc ctgctggtcg taacggtcgc aggccgaacg gatgcggtcg
    12901 agcaggacag agagctcgtg ccacggcagc atcgcctcgt cagccgtcat aatgcgcgga
    12961 tgggcggttt tgcggacgtt gtcgccgatg agcagctctt cgtagagttt ttcgccggga
    13021 cgcagtccgg taatgaggat ttcgatgtcg ccgtcgggtt gttcgggtgt gttgggtttg
    13081 aggccgctta gggtaatcat ttggcgggca aggtcgatga ttttgacgga ttcacccatg
    13141 tcgaggacga atacgtcgcc gcccgtaccc atcgcgcctg cctgtatgac gagttgggcg
    13201 gcttcgggta tggtcatgaa ataacgtgtg atttcggggt gggtcagggt aagcgggccg
    13261 ccttctgcaa tctgtttttc aaacagcggg acaacggagc cggacgaacc taaaacattg
    13321 ccgaaacgta ccatgctgaa gcgggttttt tgtccgggtt cggcggcgag tgcctgaagg
    13381 cagagttccg ccatgcgttt gctggcaccc atggtgttgg tggggcggac ggctttgtcg
    13441 gtggagatga ggacgaaagt tcttacgccc gatgtcgtgg cggcaagcgc gcactcgagt
    13501 gtgccgaaga tgttgttgcg tatgccttcg acggtgttga actcgaccat ggggacgtgt
    13561 ttgtaggcag cggcgtgata gacggtcgca acggaaaagg cggtcatgac gtgttcgagc
    13621 agcgtgcggt tttgcaccga accgagaaag ggcaggattt cggtgtcgag gcgtttttgg
    13681 atgcaggttt cgcgcaattc tttttcgatg gcgtacaggg cgaattcgga taactcgaac
    13741 agcagcagct tttcggggcg gcggcggata atctggcggc agagttccga accgatggag
    13801 ccgcccgcgc cggttaccat gacggttttg ccttcgatgt cggcactcat caggcggtcg
    13861 tcgggcgcga cggaatcacg cccgagcagg tcggacacag agattttttt gagcgtgccg
    13921 atgctgattt ttccgtccat caggtctttc attccgggaa tggtcaacac ttcgcacgga
    13981 taggcttcca gtttgttgat gattcggcgg cgttgttcct gggtcgcgcc ggggatggcg
    14041 agcaggattt tttccacgcc gtagcgttcg atgaggaagg cgatggcatc gggctggtaa
    14101 acggcaaggt cgtagatgac ggtgtgccac agtttggggt cgtcgtctac aaaggcggcg
    14161 gcggaatatt cgcgcatttg tttgacggcc tcaagcagtt gtctgcccga ccgtcccgcg
    14221 ccgtaaatga tgacagggat catctgtttt ttggggtgtt cggacaacag tccgcgcaaa
    14281 accatacgcg agccggtcac ggaaacaaac agcagtaaga aatagacaat cggcagggcg
    14341 aggcgcagcc tttcttcaaa aatcaatgtg ttgaggaaga acaggacggc ggaaacaatg
    14401 ctgccggcaa aagcggtggt caggacgtgg aaactgacaa agcgtgtaac ggcacggtag
    14461 agccccattc tgacgaacag gccgatggtc aacagcgcgg tcaataggaa agactgccag
    14521 ttggcagggt cgaaccattc gttcgagtag tcggccttta ggctttgggt gaaccagaat
    14581 gcaatgaaaa tcatcagaaa atcgtgtatg aggaaacaga ttttcttgat gttgcgcggc
    14641 agggcgatca gggtttccag agtcatatcg tggggcggta tgtgctttca ggcggcatat
    14701 gccgtctgaa gggttattgt gtggcttctg ccaatacggc ttcgatgtgt tttttgcaga
    14761 acgcgatttc gtcgtcggtc agcgtcgggt gtaccaagaa catcaggctg gtgttgccca
    14821 actcgacagc gtttttcaaa cgctctttcg gacgccaagg cgtgttgtcg aaggcttttt
    14881 ccaaatagac ttcggagcag ctgccttgat agcaggggac tttgcgcacg ttcagttcgt
    14941 tcacgatgcg gtcgcgcgtc cagccgtctt tgaggtgttc gggtttgacg aaggcgtaga
    15001 acttatattg cgcgtgttcg atgtagtcgg cgacttcaat caggcggatg cttttgaatt
    15061 tgcccaagct ttccgccagc ttggcggcgt tttcttggcg gcgcaccgtc cattcgggca
    15121 gacgtttgag ctggatgcgg ccgatgacag cctgcatttc catcatgcgc cagtttgtgc
    15181 cgaatctttc gtgcagccag cggaagccgg gggcgtgttc gcggtggtac acggcatcat
    15241 agctcttgcc gtggtctttg tacgaccaca ttttgctcca cagctctttg tcgttggtgg
    15301 tgaccatgcc gccttcgccg ccggtggtca tgattttgtc ttggcagaac gaccaagctc
    15361 caacgtggcc gatagagccg acggatttgc ctttgtattt tgcgccgtgc gcttgggcgc
    15421 agtcttcaat cacccacaaa tcatgttctt ttgccaaagc cataatgccg tccattttgg
    15481 caggcatacc ggcaaggtga accacgataa tcgctttggt attcggggtc agcgcggctt
    15541 tgacggtttc cgcgctgatg ttttggctgt tcaaatccac atcagcaaac acgggatttg
    15601 cgcccgcgtt cacaatgcag gaagcggaag ccaagaaagt gcgcgaggta acaatcacat
    15661 cgttgcccgc gcctatgccg attgctttga gcgcgacatc gagtgccagc gtgccgttgg
    15721 aaagggcgac ggcgtaccgc gtgccggcaa aggcggcaaa ttctttttca aattcgcggc
    15781 attcgttgcc cgtccagtag ttgactttgt tggacagcag gactttggaa acggcatcgg
    15841 cttcttcttg ggtgaagcag ggccacgggg aaagggcggt gttcagcatg atggtttgtc
    15901 cgtcggtttt cagacggcat ttcctcattt ctgccgtctg aagggggcgt gttccgaaga
    15961 atcgggcgcg cgccgcaggt gttgtcaaaa tcggtctgta cgggggtgta tagtggatta
    16021 aatttaaacc agtacggcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc
    16081 gtcgccttgt cctgatttaa atttaatcca ctatatttta atcgcttatg ccgtcccggt
    16141 cttggggttt ttgcgcggca gcggctttgc cggattgccc gcgacggtca tgccgtccgg
    16201 gatgtcgcac acgataaccg cccccgcgcc cattcaactt ccatcgtttc caaatggtgt
    16261 cgaaaacgta gggcggaaag ccgtttacgc cccatgaata atattttgtc atgaaatagt
    16321 ccttttaata ttgtgagtgc atgtttgatt atgtaatggt taagtatttg aatcaattat
    16381 aggaactaaa tcttgaaggg agttaaccca aaagtcggga tgatattctg tgttaaaact
    16441 ttcaggctgg tggcggtgga tgttctgtcc ggagggaagc aatcctatgg ttatccagcc
    16501 caaagtgttc ggcgcgataa agtctttgga aatgttgtcg ccgatataga tgaagcagcc
    16561 tgccttgtcg gcatatttgt cttgaagatg gcggaagcgt ttgccgtcgg gcttttccga
    16621 agagcaggct tcagagatga ggatgtcgtc aaacagggaa gacagaccca aggcttccag
    16681 cttcaggcgt tgggtcaggc ttctgccgtc ggtaatcagc gcgcgtgcgg cgaagggcgc
    16741 ggtcagttcg gacaggaaat ccggcggagc atacggagtc agcgtcgggc ggtgcagccg
    16801 gtattgccac aaaagaacct gtttttccga ttcattaaaa ccacagtggc ggcacaattt
    16861 gtccagccaa tctttgccgt cggggtcgat gttgcgccat aggttgtctg aattccaatc
    16921 gggatacagt gcggcaacgg tatcgatgac cgaacggata ccggagagct tgtattcgta
    16981 ttccgaatac agggtatcgt ccaagtcgaa gacgatgacg gtggtttcag gattaatcgt
    17041 tttcatgaac caagaccttg gcatcgtagc gcaacataat gaggttgttt tcccagtcgt
    17101 ctgaaaacgg gatttcttcg tcgccgaggt attcgcgcat caaccggctc ggataattgc
    17161 cgcctgcggc atacgtcagc gggaagccac cgccgaagcg ggggttgatt tccacgccgt
    17221 agaattcgcc ggtttcttcg ttgtagaaga actgcaccgt gatacagccg cgcgcgccct
    17281 gcaataccga cattttttcc agcagcgttt ggtagaggct gtttttgcag gtaacgcctt
    17341 tgctgatttc gccggagcgc acttccaagc gttttcgcgg gatggcgcat ttcagacggc
    17401 attgacggtc gtaatacata tcgacggtaa actcggaaaa tttattttcg atgtcgataa
    17461 actgacagaa cattaatttg gtgtctgcca agacttccgg tgtcaggtcg gcaggtgtgt
    17521 caatccgttt tgcaccgatg gcgcggctgc cgtcataagg cttggcgaag caagggaaaa
    17581 ccaacttctt gcggtcgtaa atttcgggtg agcgtatgcc gtagcgggca aacaaggtgg
    17641 cggttttccg tttgtcgcgg cattgtgcga tgaagtccga atcggaaacg acgatgtgga
    17701 cacttgccgc ttcaaagcgt tcgcgctcgt ctgccagctt ttgcagttcg gtgtctatgg
    17761 ttgggatgat taaaccgata ttttcgcgta ccgccagatt gaaaatgctg tcgatatatg
    17821 aatcggcact aatcggcggc acttcaaatg aaccgtccga aacatggcag gcagaagaca
    17881 tgcgggggtt gaggtcagtg gcaaaaacgc tgatgccgtc tgaaaaacgt gcggcttcgg
    17941 tttggaagtc ctgcaccaat tcgacacggc gacctgccga cagaatcagt atattatttt
    18001 tttcattagt ttgatttcca gtgaaataag gcatagtggc ttccccttcc gccgaaatgc
    18061 cttctttgat caagactttt ttgactgtca aaaacaggat tttcatatcc agccaaaagc
    18121 tgaagttgtc ggtgtaccaa acatcgcagg cgaacttttc gtcccacgaa agcgcgttgc
    18181 gcccgttgac ctgcgcccag ccggtaatgc cgggtttcat ttcgtggcgg cggttttgga
    18241 agttgtcgta cagcggcaga tattgcatca gcagcgggcg ggggccgacc aggctcatgt
    18301 cgcctttgag gacgttccac agttcgggca gttcgtccag gctggcggca cgcagttttt
    18361 tgccgaacgg tgtcaggcgt tctccgtcgg gcagcggaat gccgtctgaa tcaagcgcgt
    18421 cgcgcatgga acggaatttg accattttaa aaggttttcc gtcctttccg gggcgttcct
    18481 gaaagaagaa gacgggcgaa cccagattct tgcggatgag gtatatcaaa atcaaaaata
    18541 ctggcgagag gaaaatcagt cccgaggcgg aggcggcaat gtcgaacagg cgtttgaaga
    18601 atttactcat gggctaatct ttcaatcagg ttgacgattt tccgatagga aatgtcgcgc
    18661 ctgaagcggc ggacgatttc gtcggactga acggggtcgt ttttgcgttt caaaatatct
    18721 ttggcggctt gaacgaagct gtccacgtcg ccggaacggt agttttcgtg cggtagtagg
    18781 ttcaagaggc cgaggacttc ggcattggtc tgactgttta aaatcggttt ttgcaaagcc
    18841 atatagtcgg aaagtttgtt ggtaaccgat tgcatggcgt ggctatggat ggcgttgacg
    18901 gcgatgtcgc agcctttggc gatggaaatc atgtcggcgt aggggaggta gccgtaaaac
    18961 ttgatggcgc ggttttcatg ctgtttcagc ttttctaggt cggggccgcc gcccatgatg
    19021 tgcaattcga cgttttcgcc gtcgtctaaa agtttccgaa cacctttgca cacggtttcc
    19081 acgtcgtagc tgtaactgag cgttcccaag tagaaaaggc gggtggtttt ggagcggaat
    19141 cgcggggggg gggcgatggc ggcaaaatcc gtaccgatat agacggcttc gccgggtacg
    19201 ttcggattgg cttctttggc gcggtcgaga taagtctgcg atacggcaac cagcgcgtcg
    19261 gcgcagcggt aggcacggtt ggcgcgtgaa gcaaagggca gcagcttgtg cggtactttt
    19321 ttcaaaaatg gtacgaccga ggagaaagac tccggccata cgtcctgcac atcgacaatc
    19381 agtttgtagc ccaaacgcgc tttgtgtttg cccaacagca ggttggtcgc catcagcgga
    19441 taggcagaat agacgatgtc ctgttccgcc gcctgcgggc tgtgcagcca gcgtgccaaa
    19501 ttgcggacga atacgcggtg gctggcgacg cgggcgaggg agacgttttt tcggtagcca
    19561 ggttcgtcca gcagcttgac gcgcagcctg ccgtttgaag tggcggcagc atcttcatgc
    19621 cgtctgaatg atttgtcgta gtggcggaaa cggctggtaa tcagcaacac gtcgtgcgat
    19681 tgcgacaatc gttctgccag ataccaaaag cggttgaaat acggttcgga cggcagcgag
    19741 caataagggg cgactatggt gatgttcatc ggcggctttc agacggcatt acatggtata
    19801 ggccgccatg gtggtacggt agatttcgtc gtcggaacag agtgcggcga cgtgtgcgtg
    19861 cagccttttg cccattgctt cgcgcaacag cggttgtctg acgagcgtgt ctacggcggc
    19921 gataaaggct tcttcgtcgc cgaaggggat gcagtaaccc gtttccccgt tgacgaccat
    19981 ttcggaaatg cccgccatat cgtaggtaac gacgggtgtg tcgtaaagtc cggcttctaa
    20041 gatgttgttg cccacgcctg cgccgtggtc gccgatgcag tggggcgtgt tcaccaaaat
    20101 atccacgtct ttgaagtagg cggtcaaatc gcgcacgccg ccgaggaagg taactttgcc
    20161 gtctatgccc agacgttccg cctgcgcttt caaattgtcc atttcttcgc ctatgcccgc
    20221 cacgttcagg cgtacgggca tatcgcggtc gaccattttt ttgaaaatgt ccagcatcag
    20281 atgcacggcg cggatggtgt ccagacgcga cagcgtgccg agtacggcgc attctttttc
    20341 ggttttccgg aaaggaaatt ccgggggggg ggagtgcgtt gtaggcgtag acaatccggt
    20401 cggcggggaa accgtggcgg atgagttttt cgcgttcgtg tttgcagtta ccgatgatgt
    20461 acacgcccag cttgtcgaag agtttggcga ttttggggta ggttgccgcg tccaaaccgt
    20521 gcgagtggta gatgattttg gttttcggcg aaacgatttt ggcggcaagg gcgcaggctg
    20581 gaacgacgcg cgccatttgg cagtcgatga tgtcgggttg ttcgcggcgc agcatccggg
    20641 cgtaggcaaa ggtgcttttg aggtagccgg caagcccgcc ccggtaaaaa tcaaccggct
    20701 gccaacggat gcccgatgct tgcgcctctc cgacaaaagg cccgtccgaa gaagcaagga
    20761 tgatgtcgtg cccgcgccgt ttgagcagcc gcccgaggcg gacggtggcg gtttccgtgc
    20821 cgcccaagcc tgccatagat gtggtcaaaa cgattttcat aatgggaaac cttgtttttt
    20881 caaataatga aacagtttgt gcaaatcttt ccggtggcgc aggatgcagc ctgccagata
    20941 tgccgcccat acgccggcaa acagggggta gtttgccggt gtgccgaagc aggtgtaggc
    21001 cgccgaggag gccaggcaga acaatgtgtg catataaagc ggcaggcgtt tgagcggctg
    21061 ccacaggcga caggagcttt ctgtcttgaa aacaaaaaac aaccagaatg aggcggcaca
    21121 ggcaaccgcc gcgccgcgcg cgccgccgga cggtacggca agccccagca gcagcaggtt
    21181 tgccgccagc gcgcccaagg tggcgagcgc gatcgggcgt gtttttcgga cgacgttcaa
    21241 accgatgccg ctgatttcta ccagcgtgca aaacagcggc ggcagcatac acgatacgac
    21301 gataaaccgg acggcggcgt agttttccgg cagcaggagg gaggcgaggg gcgagaaaat
    21361 gccggtcagg cagagggcgg aggcaagcag ggcggcggcg gattctgccg ttgccgagag
    21421 gcgggcgggc ggggtgtttg cttcgattgc gcggaaaata tacggtgtcc agaccgttga
    21481 aaagatgctt tggaacaata atgccgcccc gccgaacgaa atacccatcg aataaacgcc
    21541 gagctgttct aggccggcat attttttcag gaacaaacgg tcggcggatg ccagccccca
    21601 ataggcgatg ctgcttagtg cgatcggtat gccgtagcgc aggccgcgat gcaggacggc
    21661 ggatgaaaac ggtgcgcgcc ggacggcctt cagacggcat cggttttgaa acagcaaaaa
    21721 ggcggcggcg gcaaggtttg ccagcgcgta aacggcggtc aggacggcgg tgttcgctgg
    21781 aaagtgcagc agcccgaccg tcagcggcag cagcagcagg atggcgagct tgggcacgag
    21841 ttgcgcggac gaaaaggcaa gggcgcgtcc ttccatacgc aaaaccagta agagaaagcg
    21901 gatgggcagg aagctcagtt caaacagcac cagcccgatg ccggcggcgg catcgtcgag
    21961 tgaaaacagg atttcagacg gcagggacgg gcgggaaagc agcagggcgg ctatcgcggc
    22021 ggcagacagc agcggcggca ggaacagggt tttgaacaag gtgtctttgt cggcggcggc
    22081 atagtattcg cggacgtatg cctgatccag cccgaggcac aataccgaca ccgtcagtcc
    22141 cgccgccgtc tgcatcagca cgatgcgccc gatgtcgtcg gcggggaaat accacgacag
    22201 cagcggcagg atgatgacgg ctaaaaccgc gctgccgatc gagcctgccg cgtagccgag
    22261 gatttctttt gtgtccattt ttgatgtccg ggcggcggcg ggatgctgcc tgtgtcgtct
    22321 gaagcctttc ttgatcggaa tttgacggct ttcagaccgt cgcggctgcc ggcggggtgc
    22381 ggcagtccgg gtcgcgcttt tccgggcggg cggcggtctg aacggggccg ttttttatcg
    22441 gc
//
LOCUS       DAWFHY010000026        22399 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.026, whole genome shotgun sequence.
ACCESSION   DAWFHY010000026 DAWFHY010000000
VERSION     DAWFHY010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.026"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(77..1246)
                     /locus_tag="WGQ68_000886"
     CDS             complement(77..1246)
                     /locus_tag="WGQ68_000886"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEZ0714718.1"
                     /translation="MSEYLFTSESVSEGHPDKVADQVSDAILDAILAQDPKARVAAET
                     LVNTGLCVLAGEITTTAQVDYIKVARETIKRIGYNSSELGFDANGCAVGVYYDQQSPD
                     IAQGVNEGEGIDLNQGAGDQGLMFGYACDETPTLMPFAIYYSHRLMQRQSELRKDGRL
                     PWLRPDAKAQLTVVYDSETGKVKRIDTVVLSTQHDPEIGYEELKNAVIEQIIKPVLPS
                     ELLTDETKYLINPTGRFVIGGPQGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVD
                     RSAAYACRYVAKNIVAAGLATQCQIQVSYAIGVAEPTSISIDTFGTGKISEEKLITLV
                     REHFDLRPKGIVQMLDLLRPIYSKSAAYGHFGREEPEFTWERTDKAAALRAAAGL"
     regulatory      complement(1429..1539)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01725"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01725"
     gene            1592..2488
                     /locus_tag="WGQ68_000887"
     CDS             1592..2488
                     /locus_tag="WGQ68_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEZ0714719.1"
                     /translation="MFRLQFGLFPPLRTAMHILLTALLKCLSLLPLSCLHTLGNRLGH
                     LAFYLLKEDRARIVANMCQAGMNPDPKTVKAVFAETAKGGLELALAFFKKPEDIETMF
                     KSVHGWEHVQQALDKGEGLLFITPHIGSYDLGGRYISQRLPFPLTAMYKPPKIKAIDK
                     IMQAGRVRGKGKTAPTSIQGVKQIIKALRSGEATIVLPDHVPSPQEGGEGVWVDFFGK
                     PAYTMTLAAKLAHVKGVKTLFFCCERLPGGQGFDLHIRPVQGELNGDKAHDAAVFNRN
                     AEYWIRRFPTQYLFMYNRYKMP"
     gene            complement(2532..3596)
                     /gene="tsaD"
                     /locus_tag="WGQ68_000888"
     CDS             complement(2532..3596)
                     /gene="tsaD"
                     /locus_tag="WGQ68_000888"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEZ0714720.1"
                     /translation="MLVLGIESSCDETGVALYDTERGLRAHCLHTQMAMHAEYGGVVP
                     ELASRDHIRRLVPLTEGCLAQAGASYGDIDAVAFTQGPGLGGALLAGSSYANALALAL
                     DKPVIPVHHLEGHLLSPLLAEEKPDFPFVALLVSGGHTQIMAVRGIGDYALLGESVDD
                     AAGEAFDKTAKLLGLPYPGGAKLSELAESGRPEAFVFPRPMIHSDDLQMSFSGLKTAV
                     LTAVEKVREANGSETIPEQTRNDICRAFQDAVVDVLAAKVKKALLQTGFRTVVVAGGV
                     GANRKLRETFGNMTVQIPTPKGKPKHPSEKVSVFFPPTAYCTDNGAMIAFAGAMHLGK
                     GREVGAFNVRPRWPLSEIVR"
     gene            complement(3713..4900)
                     /gene="ccsB"
                     /locus_tag="WGQ68_000889"
     CDS             complement(3713..4900)
                     /gene="ccsB"
                     /locus_tag="WGQ68_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HEZ0714721.1"
                     /translation="MTEHYKTLPEHELLIQKSLISNLNLWDWVFAVLVFAATVFVQTR
                     SGMHMDIYETVMLWASAGIAVFLGWFFKPMRWFVPLSVLLAYAAVGLYGGNIKSAEIF
                     LLRYFLSSQSAIMWQCAFVFFALFAYISGAVLASVKNVPTNTLLGMGTVFAWVSAVAG
                     FTGLLVRWHESYLLRPDAGHIPVSNLYEVFILFLVITALMYLYYEGKFAIQKLGGFVF
                     GFMAVVVGFVLWYSVSREAHTIQPLIPALQSWWMKIHVPANFIGYGAFCISAMLGIAE
                     LVSLRAEGKGGKLWLPPSALIDEVMYKAIAVGFLFFTIATILGALWAADAWGRYWSWD
                     PKETWAFIVWLNYAVWLHLRLVAGWRGKVLAWWAIIGLFVTAFAFIGVNMFLSGLHSY
                     GTL"
     gene            complement(4893..6845)
                     /locus_tag="WGQ68_000890"
     CDS             complement(4893..6845)
                     /locus_tag="WGQ68_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein"
                     /protein_id="HEZ0714722.1"
                     /translation="MRFAVALLSLLGIASVIGTVLQQNQPQTDYLVKFGPFWAQIFGF
                     LGLYDVYASAWFVVIMMFLVVSTSLCLIRNVPPFWREMKSFREKVKEKSLAAMRHSSL
                     LDVKIAPEVAKRYLEVQGFQGKTINREDGSVLIAAKKGTMNKWGYIFAHVALIVICLG
                     GLIDSNLLLKLGMLTGRIVPDNQAVYAKDFKPESILSASNLSFRGNVNISEGQSADVV
                     FLNADNGILVQDLPFEVKLKKFHIDFYNTGMPRDFASDIEVTDKATGEKLERTIRVNH
                     PLTLHGITIYQASFADGGSDLTFKAWNLGDASREPVVLKATSIHQFPLEIGKHKYRLE
                     FDQFTSMNVEDMSEGAEREKSLKSTLNDVRAVTQEGKKYTNIGPSIVYRIRDAAGQAV
                     EYKNYMLPVLQEQDYFWITGTRSGLQQQYRWLRIPLDKQLKADTFMALREFLKDGEGR
                     KRLVADATKDAPAEIREQFMLAAENTLNIFAQKGYLGLDEFITSNIPKEQQDKMQGYF
                     YEMLYGVMNAALDETIRRYGLPEWQQDEARNRFLLHSMDAYTGLTEYPAPMLLQLDGF
                     SEVRSSGLQMTRSPGALLVYLGSVLLVLGTVLMFYVREKRAWVLFSDGKIRFAMSSAR
                     SERDLQKEFPKHVESLQRLGKDLNHD"
     gene            complement(7122..7745)
                     /locus_tag="WGQ68_000891"
     CDS             complement(7122..7745)
                     /locus_tag="WGQ68_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c4"
                     /protein_id="HEZ0714723.1"
                     /translation="MKRLTLLAFVLAAGAVSASPKADVEKGKQVAATVCAACHAADGN
                     SGIAMYPRLAAQHTAYIYHQTIGIRDGKRTHGSAAVMKPVVMNLSDQDILNVSAFYAK
                     QQPKSGEANPKENPELGAKIYRGGLSDKKVPACMSCHGPSGAGMPGGGSEIQAYPRLG
                     GQHQAYIVEQMNAYKSGQRKNTIMEDIANRMSEEDLKAVANFIQGLR"
     gene            7951..8580
                     /locus_tag="WGQ68_000892"
     CDS             7951..8580
                     /locus_tag="WGQ68_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEZ0714724.1"
                     /translation="MNLFQNAKFFTTVNHLKDLPDTPLEIAFVGRSNAGKSSAINTLT
                     NHVRLAYVSKTPGRTQHINFFELQNGNFMVDLPGYGYAQVPEAVRAHWVNLLGDYLQQ
                     RKQLIGLVLIMDARHPLKELDIRMLDFFHTTGRPVHILLSKADKLSKNEQIKTLSQVK
                     KLLKPYSDRQNISVQLFSSLKKQGIDEANRTVGSWFDAADAAASSPKEN"
     gene            complement(8702..11098)
                     /locus_tag="WGQ68_000893"
     CDS             complement(8702..11098)
                     /locus_tag="WGQ68_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HEZ0714725.1"
                     /translation="MIKKILTTCFGLVFGFCVFGVGLVAIAILVTYPKLPSLDSLQHY
                     QPKMPLTIYSADGEVIGMYGEQRREFTKIGDFPEVLRNAVIAAEDKRFYRHWGVDVWG
                     VARAAVGNVVSGSVQSGASTITQQVAKNFYLSSEKTFTRKFNEVLLAYKIEQSLSKDK
                     ILELYFNQIYLGQRAYGFASAAQIYFNKNVRDLTLAEAAMLAGLPKAPSAYNPIVNPE
                     RAKLRQKYILNNMLEEKMITVQQRDQALNEELHYERFVRKIDQSALYVAEMVRQELYE
                     KYGEDAYTQGFKVYTTVRADHQKVATEALRKALRNFDRGSSYRGAENYIDLSKSEDVE
                     ETVSQYLSGLYTVDKMVPAVVLDVTKKKNVVIQLPGGRRVTLDRRALGFAARAVNNEK
                     MGEDRIRRGAVIRVKNNGGRWVVVQEPLLQGALVSLDAKTGAVRALVGGYDFHSKTFN
                     RAVQAMRQPGSTFKPFVYSAALSKGMTASTVVNDAPISLPGKGPNGSVWTPKNSDGRY
                     SGYITLRQALTASKNMVSIRILMSIGVGYAQQYIRRFGFRSSELPASLSMALGTGETT
                     PLKVAEAYSVFANGGYRVSSHVIDKIYDRDGRLRAQMQPLVAGQNAPQAIDPRNAYIM
                     YKIMQDVVRVGTARGAAALGRTDIAGKTGTTNDNKDAWFVGFNPDVVTAVYIGFDKPK
                     SMGRVGYGGTIAVPVWVDYMRFALKGKQGKGMKMPEGVVSSNGEYYMKERMVTDPGLT
                     LDNSGIAPQPSRRAKEDDGGAAEGGRQAADDEVRQDMQETPVLPSNTGSKQQQLDSLF
                     "
     gene            11250..12365
                     /locus_tag="WGQ68_000894"
     CDS             11250..12365
                     /locus_tag="WGQ68_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HEZ0714726.1"
                     /translation="MRLFKSLKNPKKTDAKLPKKSSGLNNRAAIGIDIDQHSIKMVQL
                     SGRSLNQIQLEKYVIAKLPKNIIQGNKVQNYDQLVTYLQQAYAKLGTSCKNIIASVPQ
                     NLATIEQLTYTDKDAELDLQGFVESSISEVSSISLEEANYDYQVLSQSAAGEAVLAVA
                     SRKDEIEPLIDAFNAAGMKLSALDVDIFGQYNAYALWINHFAPELAAEKVAIFGVYAA
                     QTYALVIQDGKILYKQETSVSEEQLNQLIQRTYQVTEEKAEEIINSPQKPSDYQESVA
                     NYFNQQITQEIQRVLQFYYTTQTADDMTDIKHILLTGEAARQEGIAQTVASQTNADVQ
                     CVHPARYFADNLKTDKQQFELDAPTLTRAFGLAVRGL"
     gene            12368..12967
                     /locus_tag="WGQ68_000895"
     CDS             12368..12967
                     /locus_tag="WGQ68_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEZ0714727.1"
                     /translation="MNNLIKINLLPYREEMNKRKQQQFKTLMYGAVLTGVAAVAATYL
                     FIDNMINNQSERNTLLETSIAHLDTELSEIQKLKQEKDAFLIKKNKIEELQLKRLQAA
                     KILDSLNEAVPGSTYLTSLDAVTADSYRLSGRTSSDNRVAAMMRAMPNTGIFKQPELL
                     SIKKNNSHQEFTLQATLQPIVKAAESKENPASGNAQEAN"
     gene            12968..13615
                     /locus_tag="WGQ68_000896"
     CDS             12968..13615
                     /locus_tag="WGQ68_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEZ0714728.1"
                     /translation="MASKSSKTNLDLNNLHLLNLPARLFIALLAVAAVLGLGYAGLFK
                     SQMESLEEYEAKETELKNTYKQKSIDAASLNNLRDELASIRSAFDIMLKQLPTDAEIP
                     NLVQELHQAGSSNGLRLDSVMPQPPVDDGPIKRLPYSISITGNYEQISQFTRDVGSLS
                     RIITLESLKIAQSPENGGNPDGKSSILNLSAIATTYQAKSVEELAAEAAQNAEQK"
     gene            13633..14178
                     /locus_tag="WGQ68_000897"
     CDS             13633..14178
                     /locus_tag="WGQ68_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HEZ0714729.1"
                     /translation="MKHYALLISFLALSACSQGSEDLNEWMAQTRREAKAEIIPFQAP
                     TLPVAPVYSPPQLTGPNAFDFRRMETDKKGENAPDTKRIKETLEKFSLENMRYVGILK
                     SGQKVSGFIEAEGYVYTVGVGNYLGQNYGRIESITDDSIVLNELIEDSTGNWVSRKAE
                     LLLNSSDKNTEQAAAPAAEQN"
     gene            14197..16482
                     /locus_tag="WGQ68_000898"
     CDS             14197..16482
                     /locus_tag="WGQ68_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HEZ0714730.1"
                     /translation="MNTKLTKIISGLFVATAAFQTASAGNITDIKVSSLPNKQKIVKV
                     SFDKEIVNPTGFVTSSPARIALDFEQTGISMDQQVLEYADPLLSKISAAQNSSRARLV
                     LNLNKPGQYNTEVRGNKVWIFINESDDTVSAPARPAVKAAPAAPAKQQAAAPSTKSAV
                     SVSKPFTPAKQQAAAPFTESVVSVSAPFSPAKQQAAASAKQQTAAPAKQQAATPAKQT
                     NIDFRKDGKNAGIIELAALGFAGQPDISQQHDHIIVTLKNHTLPTTLQRSLDVADFKT
                     PVQKVTLKRLNNDTQLIITTAGNWELVNKSAAPGYFTFQVLPKKQNLESGGVNNAPKT
                     FTGRKISLDFQDVEIRTILQILAKESGMNIVASDSVNGKMTLSLKDVPWDQALDLVMQ
                     ARNLDMRQQGNIVNIAPRDELLAKDKAFLQAEKDIADLGALYSQNFQLKYKNVEEFRS
                     ILRLDNADTTGNRNTLVSGRGSVLIDPATNTLIVTDTRSVIEKFRKLIDELDVPAQQV
                     MIEARIVEAADGFSRDLGVKFGATGKKKLKNDTSAFGWGVNSGFGGDDKWGAETKINL
                     PITAAANSISLVRAISSGALNLELSASESLSKTKTLANPRVLTQNRKEAKIESGYEIP
                     FTVTSIANGGSSTNTELKKAVLGLTVTPNITPDGQIIMTVKINKDSPAQCASGNQTIL
                     CISTKNLNTQAMVENGGTLIVGGIYEEDNGNTLTKVPLLGDIPVIGNLFKTRGKKTDR
                     RELLIFITPRIMGTAGNSLRY"
     gene            17660..18172
                     /locus_tag="WGQ68_000899"
     CDS             17660..18172
                     /locus_tag="WGQ68_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEZ0714731.1"
                     /translation="MKNFNGKLILIGLMGAGKTTLGRQMAQRLDYRFYDSDHEIAAAA
                     GVPIPTIFEMEGEQGFRSRETAILKKLVILPHIVLSTGGGAVLKEENRALIRKSGTVV
                     YLHAPPETLLERTRCDNSRPLLQVADPLAKLRELYAARDPVYRQTADFTVESANCRET
                     VQTLLKRLSR"
     gene            18252..19331
                     /gene="aroB"
                     /locus_tag="WGQ68_000900"
     CDS             18252..19331
                     /gene="aroB"
                     /locus_tag="WGQ68_000900"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEZ0714732.1"
                     /translation="MKTLTVHTPSHSYPIFIGNGLLPQAGSLLKPHLGKRAAIIANET
                     VAPLYLGTLQTALDAAGVSHFSIILPDGEAHKNWQTLNLIFDGLMQNRAERKTTLIAL
                     GGGVIGDMVGFAAATYQRGAPFVQIPTTLLSQVDSSVGGKTAINHPLGKNMIGAFYQP
                     QAVLADLDTLHTLPVRELSAGMAEVIKYGALGDIGFFEWLEQNISGLMTLDREKLAQA
                     VYRCCQMKADIVAQDETEQGIRAWLNLGHTFGHAIETEMGYGTWLHGEAIAAGCVLAA
                     RLSEQLGKTSAADTARLAALLEAAGLPSAPPVFAFEKWLEHMSHDKKVSGGIMRFIGL
                     NRLGEANITEITDTDILRRTLQPYL"
     gene            19898..20740
                     /locus_tag="WGQ68_000901"
     CDS             19898..20740
                     /locus_tag="WGQ68_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HEZ0714733.1"
                     /translation="MCQLLGMNCNTPTDIMFSFEGFRRRGGITDHHADGFGIGFFEGK
                     GVRLFHDDKPSANSPVADLVRAYQIKSENVVAHIRKASQGQTSLANTHPFMREMWGGY
                     WLFAHNGHLIDFFPEQGEFFHPVGTTDSERAFCHILNRLRTRFAARPDDDTLFDAIAG
                     LTHEIRKFGLFNFMLSDGIALFAHASTLLHYIVRQAPFGKARLLDDDVMVDFAEVTTP
                     SDRVAVIATLPLTRDETWAQLAVDELVMFREGNIVRHDRPENPVYMSVEEGLETARAA
                     GVAV"
     gene            20770..21234
                     /gene="nrdR"
                     /locus_tag="WGQ68_000902"
     CDS             20770..21234
                     /gene="nrdR"
                     /locus_tag="WGQ68_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEZ0714734.1"
                     /translation="MKCPFCAHPDTRVADSRLMDERNAVRRRRYCPNCGKRFGTLETA
                     ELKMPAVIGPDKKRSPFNAQRLRNDLTAAARKSALTPEQIDETVRLTELRLYASGLRD
                     ISAAELVEIVLEELFGQSTEAAVRFAALHKRFDNPADFASWLAQAVKTGGKA"
     gene            21266..22375
                     /gene="ribD"
                     /locus_tag="WGQ68_000903"
     CDS             21266..22375
                     /gene="ribD"
                     /locus_tag="WGQ68_000903"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEZ0714735.1"
                     /translation="MFSDTDISMMENALRLAALGRFSTSPNPRVGCVIAQGSQIVGQG
                     FHVKAGEPHAEVHALRQAGEMAQGATAFVTLEPCSHYGRTPPCAEALLRSGVTRVVAA
                     MRDPNPLVAGKGLALLEAAGIKTECGLLEHQARELNRGFLSRIERRRPFVRLKCAVSL
                     DGKTALSDGSSFWITGEDARADVQVLRAESCAVLTGIGTVLADNPRLNVRAFPTLRQP
                     ARIVLDSRLRLPLDSHLLTDGQSPTYIATLERNEDRLHPYREHAHVRILMPSETADSK
                     IDLNHLMRLLADEGFGEIMVEAGSELTSAFLAEDLADEIVLYRSPKILGSGKDLFSLP
                     ENRAALSAPPLWMPVSTEILGHDIKTVFRKNDNAF"
BASE COUNT         5837 a         6513 c         5198 g         4851 t
ORIGIN      
        1 cctgattttt gttaatccac tatataaaaa tgccgtctga actgttcaga cggcattttt
       61 gattttcaaa ccggaattac agccccgctg ccgccctcaa tgcagcagct ttgtcggtgc
      121 gctcccaagt gaactcaggt tcttcgcggc cgaaatgtcc gtaagcggcg gatttactgt
      181 aaatcgggcg caagaggtcg agcatttgga cgatgccttt ggggcgcagg tcgaaatgtt
      241 cgcgaactaa ggtaatcagt ttttcttcgc tgattttgcc tgtgccgaaa gtatcgatgg
      301 aaatcgaagt cggttcggca acgccgatgg cgtaggaaac ttggatttgg cattgggttg
      361 ccaagcctgc ggcgacgatg tttttcgcca catagcggca ggcgtaggcg gcggaacggt
      421 caaccttgga cggatctttg ccggagaacg cgccgccgcc gtgcggagcc gcgccgccgt
      481 aggtatcgac gatgatttta cggccggtca aaccgcagtc gccttgcggg ccgccgatga
      541 cgaagcggcc ggtcgggttg atcaggtatt tggtttcgtc ggtcagcagt tcagacggca
      601 ggacgggctt gataatctgc tcaatgacgg cgtttttcaa ctcttcataa ccgatttccg
      661 gatcgtgttg cgtagagagg acgacggtat caatgcgttt taccttaccg gtttcgctgt
      721 cgtaaaccac ggtcagttgg gctttggcat caggacgcag ccacggcagg cggccgtctt
      781 tgcgcaattc gctttggcgt tgcatcaggc ggtggctgta atagatggca aacggcatca
      841 gggtcggggt ttcgtcgcag gcgtagccga acatcagacc ttggtcgcct gcgccttggt
      901 tcaagtcgat gccttcgcct tcgtttacgc cttgggcaat gtccggagat tgttggtcgt
      961 agtacacgcc gaccgcgcag ccgttggcat caaagcccag ctcggaggag ttgtagccga
     1021 tgcgtttgat ggtttcgcgt gcgactttga tgtagtccac ttgggcggtg gtggtgattt
     1081 cgcccgccaa tacgcacaag cctgtgttga ccaaagtttc tgcggcgaca cgcgctttgg
     1141 ggtcttgcgc cagaatcgca tccaaaatag catcggatac ttggtcggcc actttgtccg
     1201 gatggccttc ggataccgat tcggaagtaa acagatattc gctcattgag attttccttg
     1261 aaaaacaaac catcttcgtt agacggcatg ttgtatgaac ataatgtcga cagcgggaaa
     1321 tatagcaaaa tttccctatt cgtgccattc agttgagaaa cattcccatt tgaatggcac
     1381 tttggaatct ctgcccgtac gtttcgtaca ggcaaaaaaa ttcccgcatc aagcgggttt
     1441 ggattgctct ggtgagccac atcggctttt caaccgtcca ccttactttt ccttttgaaa
     1501 agcaggttgg catggaattc ccaactctta atgcagcgcg gatggtagca gaaaagcctt
     1561 tccgccgcaa tacttccctt tttcagacgg catgtttcgt ttacaattcg ggctgtttcc
     1621 ccctttgcga accgccatgc acatcctgtt gaccgccctg ctcaaatgcc tctccctgct
     1681 gccgctttcc tgtctgcaca cgctgggaaa ccggctcgga catctggcgt tttacctttt
     1741 aaaggaagac cgcgcgcgca tcgtcgccaa tatgtgtcag gcaggcatga atcccgaccc
     1801 caaaacggtc aaagccgttt ttgcggaaac ggcaaaaggc ggtttggaac ttgccctcgc
     1861 atttttcaaa aaaccggaag acatagaaac aatgttcaag tcggtacacg gctgggaaca
     1921 cgtgcagcag gctttggaca agggcgaagg gctgctgttc atcacgccgc acatcggcag
     1981 ctacgatttg ggcggacgct acatcagcca acggctgcca ttccccttaa ccgccatgta
     2041 taaaccgccg aaaatcaaag cgatagacaa aatcatgcag gcgggcaggg ttcgcggcaa
     2101 aggaaaaacc gcgcctacca gcatacaagg ggtcaaacaa atcatcaaag ccctgcgttc
     2161 gggcgaagca accatcgtcc tgcccgacca cgtcccctcc cctcaagaag gcggggaagg
     2221 cgtatgggtg gatttcttcg gcaaacctgc ctataccatg acgctggcgg caaaattggc
     2281 acacgtcaaa ggcgtgaaaa ccctgttttt ctgctgcgaa cgcctgcctg gcggacaagg
     2341 tttcgatttg cacatccgcc ccgtccaagg ggaattgaac ggcgacaaag cccatgatgc
     2401 cgccgtgttc aaccgcaatg ccgaatattg gatacgccgt tttccgacgc agtatctgtt
     2461 tatgtacaac cgctacaaaa tgccgtaacg aaaataaaaa tgccgtctga acaatttcag
     2521 acggcatttt gtcatctgac gatttccgac agcggccagc gcggacggac attgaacgca
     2581 ccgacctccc tgcccttgcc caggtgcatc gcaccggcaa aggcaatcat ggcaccgttg
     2641 tccgtgcagt atgccgtcgg cgggaaaaac acgctgactt tttcggacgg atgtttcggc
     2701 ttgccttttg gggtcgggat ttgcaccgtc atattgccga aagtttcacg gagtttgcgg
     2761 tttgcaccga ccccgccggc gaccactacg gttctgaacc ctgtctgcaa cagggctttt
     2821 ttcactttcg ccgccaacac atcgactacc gcatcttgaa acgcacggca aatatcgttg
     2881 cgggtttgct cgggaatggt ttccgaaccg tttgcctcac gcactttttc aacggcagtc
     2941 aaaacagcgg ttttcaaacc tgaaaaactc atctgcaaat cgtcggaatg aatcatcggg
     3001 cgcggaaaaa caaacgcttc gggcctgccc gattccgcaa gttccgacag tttcgcaccg
     3061 cccggatacg gcaagcccag cagtttcgcc gttttgtcga atgcctcgcc cgccgcatca
     3121 tcgacgctct cgcccaaaag cgcgtagtcg cctatgcccc tgaccgccat aatctgcgta
     3181 tgcccgcccg aaaccaacag cgcgacaaaa ggaaagtcgg gtttttcctc cgccaacagc
     3241 ggcgacagca gatgtccttc caaatgatgg acgggaataa caggcttgtc caacgctaaa
     3301 gccagcgcat tggcgtaact cgaacccgcc agcaacgcac cgcccaaacc gggcccctgc
     3361 gtaaaggcaa ccgcgtcaat gtcgccatac gatgcgcccg cctgcgccaa acagccttca
     3421 gtcaacggaa caaggcggcg gatatggtcg cggcttgcca attccggcac aaccccgccg
     3481 tattcggcgt gcattgccat ttgagtgtgc aggcagtgcg cccgcaatcc acgttccgta
     3541 tcgtaaagcg caacacctgt ttcgtcgcaa gaagactcga ttcctaatac caacatggtc
     3601 tgatgccgtt aaaaactgaa aaacgtattt tagcggattt cggcacgact gccgtatccc
     3661 aaaaacggaa catgccgtct gaagaccgtt cagacggcat cgtcgcaccg tatcaaagcg
     3721 ttccgtaaga atgcagcccg ctcaaaaaca tattcacgcc gataaaggca aatgcggtta
     3781 cgaacaaacc gataatcgcc caccacgcca gcactttgcc gcgccaaccg gcaaccagcc
     3841 gcaagtgcag ccaaacggcg taattgagcc agacgatgaa cgcccacgtc tctttcggat
     3901 cccaactcca atagcgtccc caagcatctg ccgcccacag cgcacccaaa atggtggcaa
     3961 tggtaaagaa cagaaagccg acggcaatcg ccttatacat cacctcgtcg atcaatgccg
     4021 acggcggcag ccacagtttt ccgccttttc cttccgcacg cagggaaacc agttcggcaa
     4081 taccgagcat cgcggaaatg caaaacgcgc cgtaaccgat aaagtttgcc ggaacgtgga
     4141 ttttcatcca ccaggactgg agcgcgggaa tcagcggctg gatggtatgc gcctcgcggg
     4201 acacgctgta ccacaagaca aatccaacca cgaccgccat aaagccgaac acgaagccgc
     4261 ccaatttctg tatggcgaac ttaccttcat aataaagata catcagcgcg gtaatcacca
     4321 aaaacaggat gaacacttca tacaggttgg aaaccggaat atgccccgca tcgggacgga
     4381 gcagatagct ttcgtgccaa cgtaccagca gaccggtaaa gcctgctacg gcagacaccc
     4441 atgcaaacac ggttcccata cccaacagcg tgttggtcgg cacatttttt acgcttgcca
     4501 aaaccgcgcc cgaaatatag gcgaacaggg cgaaaaagac aaaggcgcac tgccacatga
     4561 tcgccgactg gctgctgagg aaataccgca acaggaaaat ctctgccgat ttgatgttgc
     4621 cgccatacag accgacggcg gcataggcaa gcaatacgct taaaggaaca aaccagcgca
     4681 tcggtttgaa aaaccaaccc aaaaacacgg caataccggc actcgcccac aacatgaccg
     4741 tttcgtaaat gtccatatgc ataccggaac gggtctgtac gaaaaccgta gccgcaaaaa
     4801 ccagcacggc aaatacccaa tcccaaagat tcagattgct gatcaaagac ttctgaatca
     4861 gcagctcgtg ttccggaagg gttttatagt gttcagtcat gattcaagtc cttgccgagc
     4921 cgttgcagac tctcgacgtg ttttggaaat tccttctgca aatcccgttc gctgcgggcc
     4981 gaagacatgg caaaacggat tttgccgtct gaaaacaata cccacgcccg tttttcgcgc
     5041 acataaaaca tcaataccgt acccaatacc aacagcaccg agccgagata gaccaaaagc
     5101 gcacctgggg aacgggtcat ctgcaaaccc gacgaacgca cctcggaaaa cccatcaagt
     5161 tgcagcagca taggcgcggg atattcggtc aaacccgtgt acgcatccat actgtgcagc
     5221 aggaaacggt tccgcgcttc atcctgctgc cattcgggca agccgtaccg gcgtatggtt
     5281 tcatccaaag cagcgttcat cacgccgtaa agcatttcgt agaaataacc ctgcatctta
     5341 tcctgctgct ctttcgggat attggacgta ataaattcgt ccaatcccaa atagcctttt
     5401 tgtgcaaaga tgttcagcgt gttttccgca gccagcatga attgttcgcg gatttcggca
     5461 ggtgcgtctt tggttgcgtc ggcaaccaga cgtttgcgcc cttccccatc tttcaaaaac
     5521 tcacgcaatg ccataaaggt gtccgctttc aactgcttgt ccaaggggat acgcagccag
     5581 cggtattgct gctgcaagcc gctgcgcgtg ccggtaatcc aaaaataatc ctgttcctgc
     5641 aaaaccggca gcatatagtt tttatattcg accgcctgcc ctgccgcatc acggatacgg
     5701 taaacaatgg aagggccgat attggtgtat tttttacctt cctgagtaac ggcgcggaca
     5761 tcgttcagcg tggatttcag gcttttttcc cgttccgcgc cctcgctcat gtcctccaca
     5821 ttcatagaag tgaactgatc gaactcaaga cgatatttgt gtttgccaat ttccaacgga
     5881 aactggtgta tggatgttgc cttcaacacg acaggctcgc gcgaagcatc acccaaattc
     5941 cacgccttga atgtcaaatc cgaaccgccg tcggcaaaac tcgcctgata aatcgtgatg
     6001 ccgtgcaagg tcaaaggatg gttcacgcgg atggtgcgct cgagtttctc accggttgcc
     6061 ttgtccgtca cttcaatatc gctggcgaaa tcacgcggca tacccgtatt gtaaaaatcg
     6121 atatggaatt ttttcagttt gacttcaaaa ggcaagtcct gaaccaatat cccgttgtcg
     6181 gcattcagga aaaccacatc cgcactctgc ccctcggaaa tattgacgtt gcccctaaat
     6241 gagagattgg acgcactcaa aatactttcg ggcttgaaat ccttggcata aaccgcctga
     6301 ttgtccggaa caatccgacc ggtcagcata cccagtttca acagcaggtt actgtctatc
     6361 aacccgccca ggcaaatgac aatcaaagca acatgggcaa agatatagcc ccatttgttc
     6421 attgtgcctt ttttggcggc aatcagaacc gacccgtctt cacggttaat ggtttttccc
     6481 tgaaaacctt gtacttccag ataacgtttg gcaacctcgg gcgcaatttt tacatccaac
     6541 agcgaagaat ggcgcatcgc cgccagagat ttttctttaa ccttttcccg aaaagacttc
     6601 atttcgcgcc agaacggcgg cacattgcga atcaggcaca aactggtaga aaccaccaaa
     6661 aacatcatga taacgacaaa ccatgccgaa gcatagacgt catacagtcc cagaaaacca
     6721 aaaatctgcg cccaaaaagg tccgaatttg accaaataat ccgtctgcgg ctggttttgc
     6781 tgcaacaccg taccgataac cgatgcaata cccagcagac tgagcaaagc gaccgcaaag
     6841 cgcatggagc tgaaaaaagc gaaccacgga cgggaaagaa gtgggggaga tctacgggat
     6901 ttactcattg tgtgtttatt ccgccatcag gaatatggga aagcagaatt gggcaaacag
     6961 aaaacaacgt cccgattcta ctgtcttgat gctttttatt tcaagacaat gaagacagcc
     7021 tgcatcgatt ccaacggttg cgattgaaaa aacttatcgc agaattgcct gaagccgtct
     7081 gaaaactttt cagacggcct ctaaaacaga ctattgcgga attaacgcaa accttggata
     7141 aagttggcga ccgctttcaa atcttcttca gacatacggt ttgcaatatc ttccatgatg
     7201 gtatttttac gctgaccgga cttgtaggca ttcatctgtt caacaatata tgcctgatgc
     7261 tgaccgccca aacgcggata agcctgaatt tcgcttccgc ctcccggcat acccgcaccg
     7321 ctcggaccgt ggcaggacat acacgccggc acttttttat cgctcaaacc gccgcgatag
     7381 attttcgcac ccaattcggg attttcctta ggattggctt caccggattt gggctgctgt
     7441 ttggcataga atgcggatac gttcaaaata tcctgatcgc tcaaattcat taccaccggt
     7501 ttcatcacag ctgccgaacc gtgggtgcgt ttaccgtcgc ggatgccgat agtttgatga
     7561 tagatgtaag cagtatgctg tgccgccaaa cgcggataca tcgcaatgcc gctgttaccg
     7621 tctgctgcat ggcaagccgc acaaaccgtt gcggcaacct gtttgccttt ttccacgtct
     7681 gctttgggag aggcggaaac cgcaccggca gccaaaacaa aggccaataa agtcaatcgt
     7741 ttcatggagt gctcctgatt acagcattgg ataacgcaac aatgctcttt ttatattcaa
     7801 atacgggatt tttgacccga ttaaaaccga tgattctgta aacgtgttat tctatactaa
     7861 atttacatta aattaccact gtgtttcaca taaaaccaac cgcatatttt tgctgtcgga
     7921 caaacggcgg cggaaaacaa ggatatgccc atgaaccttt ttcaaaacgc caaattcttc
     7981 acgacggtaa accatctcaa agacctgccc gacacccctc tcgaaattgc ctttgtcggc
     8041 aggagcaatg ccggaaaatc cagtgccatc aataccctga ccaaccatgt ccgtcttgcc
     8101 tacgtttcaa aaacacccgg acggacgcag catatcaact tcttcgagct gcagaacggc
     8161 aattttatgg tcgatttgcc cggctacggt tatgcccaag tccccgaagc agtacgcgca
     8221 cattgggtca atctgctcgg cgactatctg caacagcgca aacagcttat cgggctggtt
     8281 ttgattatgg atgcccgcca tcctttaaaa gaactcgaca tccgtatgct ggattttttc
     8341 cacacgaccg gcagaccagt tcacatcctg ctgtcaaaag ccgacaaatt atccaaaaac
     8401 gaacagataa aaaccctgtc ccaagtcaaa aaactgctca aaccttattc cgacaggcaa
     8461 aacatcagcg tacagctgtt ttccagcctg aaaaaacaag gtattgacga ggccaaccga
     8521 actgtcggaa gctggtttga cgcagcagat gccgccgctt cctctccaaa ggaaaactga
     8581 ccccaattat acggaaaccg tattcccccc cacttgaccg accgcaaaca tttaaaaaat
     8641 tgccactgcc aaatctaaaa tgccgtctga aaagtctttc agacggcatt ttgcggagtc
     8701 tttaaaacag agaatccaac tgctgctgtt tggaaccagt attactcgga agcaccggcg
     8761 tttcctgcat atcttggcgg acttcgtcat ccgccgcctg ccgtccgcct tctgccgcgc
     8821 ccccgtcatc ttcttttgcc cgtcgggaag gttgcggcgc aataccgctg ttgtccagcg
     8881 tcaagcccgg atcggttacc atacgttcct tcatatagta ttcgccattg ctgctgacca
     8941 caccttcagg cattttcatc cccttgccct gctttccttt caacgcaaaa cgcatatagt
     9001 ccacccaaac cggcaccgca atcgtaccgc cgtagccgac acgccccata ctcttaggtt
     9061 tgtcgaagcc gatatatacg gcagtaacca catcagggtt aaaaccgaca aaccacgcat
     9121 ccttattgtc attggtcgta cccgttttac cggcaatatc cgttcttccc aacgcagctg
     9181 ccccccttgc cgtaccgaca cggaccacat cctgcataat cttatacata atataagcat
     9241 tgcgcggatc gattgcctga ggcgcatttt gcccggccac caaaggttgc atttgggcgc
     9301 gcaacctgcc gtctctgtca taaatcttat cgattacgtg cgaagaaacc ctatatccgc
     9361 cgttcgcaaa tacgctatat gcctccgcca ctttcaacgg cgttgtctcg cccgtaccta
     9421 aagccataga caggcttgcc ggcagctcgg acgacctgaa gccgaaacgc cggatatact
     9481 gttgcgcgta accgacaccg atagacatca aaatacggat ggaaaccata ttcttggaag
     9541 ccgtcagagc ctgtctcaaa gtaatgtagc cggaatatct gccgtctgaa tttttaggtg
     9601 tccaaaccga accgttcggc cctttccccg gcagggaaat cggcgcatcg ttaaccactg
     9661 tggacgcggt catcccctta gataatgccg ccgaatagac aaacggctta aaggtcgaac
     9721 ccggctgccg cattgcctga acggcacgat tgaatgtttt gctgtgaaaa tcataaccgc
     9781 cgaccagcgc gcgcacagct ccggtttttg catccagcga aaccaaagcc ccctgcagca
     9841 acggctcttg aaccaccacc caacgcccgc cgttgttttt gacacggatg accgcgcccc
     9901 tgcggatacg gtcctccccc attttttcat tattgaccgc gcgggccgca aaacccaagg
     9961 cgcgcctgtc aagcgtaacc cgcctgccgc cgggcagctg tatgacgaca tttttctttt
    10021 tagtcacatc caacacaacg gcgggaacca ttttatcgac ggtatagagt cccgacagat
    10081 actggctgac agtctcctcg acatcttcac tcttactcaa atcgatatag ttttccgcac
    10141 cgcggtagct gctgccgcga tcgaaattcc gtagagcctt gcgcaatgcc tcggttgcca
    10201 ccttctgatg atcggcgcgg accgtggtat aaaccttaaa accctgcgta taggcatctt
    10261 caccgtattt ctcatacagt tcctgacgca ccatttccgc cacatataaa gcactctgat
    10321 cgattttccg aacaaaccgc tcgtaatgca gttcctcatt caacgcctga tcgcgctgtt
    10381 gcacggtaat catcttctcc tcgagcatat tgttcaaaat atacttctgg cgcaacttgg
    10441 cacgttccgg attaacaatc ggattatagg cagacggagc cttgggcagt cccgcaagca
    10501 tggcggcttc cgccaaagtc aaatctcgga cattcttatt gaaatagatt tgcgcggcag
    10561 atgcaaaacc ataggcgcgc tgaccgaggt aaatctgatt gaaatacaac tcgaggattt
    10621 tgtctttgct taaagactgc tcgattttat aggcaagcaa cacctcattg aatttgcgtg
    10681 tgaacgtttt ttcactgctc aaataaaaat ttttcgccac ctgctgcgta atcgtactcg
    10741 cacccgactg cacgctgccg gacacgacat tgccgacggc agcgcgggca acaccccaaa
    10801 catccacccc ccaatgccgg taaaagcgtt tatcctcggc ggcgataacc gcattccgca
    10861 acacctctgg gaaatcgccg atttttgtaa attcgcgccg ctgctcccca tacataccga
    10921 tgacttcccc atccgccgaa taaatagtca acggcatttt aggctggtaa tgctgcaaag
    10981 aatccaaaga cggtagtttc ggatacgtta ccaaaatagc aatggcaacc aaacccactc
    11041 caaatacaca aaacccaaaa accaaaccaa aacaagtcgt taaaatcttt ttaatcatag
    11101 ctgaataata atttaccatt attggtatta aataaagtaa aatagcaacc gatttctaca
    11161 aagcacggtt tcaatgtgca aagaacaagg aatccattac ggataccgaa acggttactc
    11221 actgtacaaa taaagcagga actttcatca tgcgcttgtt taaaagcttg aaaaacccta
    11281 aaaaaacaga tgccaagctc cctaaaaaat cttcgggact caataaccgc gcggcaatcg
    11341 gcatcgatat cgaccagcat tccatcaaaa tggtccaatt gtcaggacgt agtttaaacc
    11401 aaattcaatt ggaaaaatac gtcattgcca aattaccaaa gaatatcatt caaggcaata
    11461 aagtccaaaa ttacgatcaa cttgttacat atttgcaaca agcctatgcc aaactgggta
    11521 cttcgtgcaa aaacatcatc gcgtccgtcc cgcaaaattt ggcaaccatc gagcaattga
    11581 cctacacaga caaagatgca gaattagacc tgcaggggtt cgtggagtcc tccatctccg
    11641 aagtcagctc gatatcgctc gaagaagcca attacgacta tcaggtcttg tcccaatcgg
    11701 ccgccggcga agctgtgttg gccgtcgcat cgagaaagga tgaaatcgaa cccctgattg
    11761 acgcattcaa cgccgccggt atgaaattat ccgcgcttga tgtggacatt ttcggacaat
    11821 acaacgccta cgcgctatgg ataaaccatt tcgcccccga gcttgcagcc gaaaaagtcg
    11881 ccattttcgg cgtatatgcc gcacagacct acgccttggt catccaagac ggaaaaatcc
    11941 tatacaaaca ggaaacctcc gtcagcgaag aacagctcaa ccaactcatc cagcgcacct
    12001 atcaggtaac agaagaaaaa gcggaagaaa tcatcaactc cccgcaaaaa ccttccgatt
    12061 accaagaaag cgtggcaaac tatttcaacc agcagattac ccaagaaata caaagggtct
    12121 tgcagtttta ttacaccacg cagaccgcag acgatatgac cgacatcaag catatcctgc
    12181 tgaccgggga agcggcgcgc caggaaggca tcgcccaaac cgtcgcctca caaaccaatg
    12241 cagatgtaca atgcgtccat cccgcgcgtt attttgcgga caacctcaaa acagacaaac
    12301 aacaattcga acttgatgcg ccgacactga ccagggcgtt cggtttggcg gtacggggat
    12361 tataattatg aacaatttaa tcaaaatcaa cctcctcccc tacagggaag agatgaacaa
    12421 gcgcaaacag cagcagttta aaacgctgat gtacggtgcc gtgctgacgg gcgttgccgc
    12481 cgttgcggca acctacctgt ttatcgacaa tatgatcaat aaccagtcgg aaagaaacac
    12541 gctgctggaa acctccatcg cacacttgga taccgagctg tcggaaatac aaaagctcaa
    12601 acaggaaaaa gatgccttcc tgattaagaa aaacaaaatc gaggagctcc agctcaaacg
    12661 cctccaagcc gcaaaaatcc tcgacagcct gaatgaggcc gtccccggaa gcacctacct
    12721 gacctcgctg gatgccgtta ccgccgactc ttatcggctc agcggcagga catccagcga
    12781 caaccgcgtt gccgccatga tgagggcgat gcccaatacc ggcatattca agcaacccga
    12841 attgttaagc atcaagaaaa acaattcgca tcaagaattt acccttcagg caacattaca
    12901 acccatcgta aaggcggccg aatccaaaga gaatccggct tcgggaaacg cacaggaggc
    12961 aaactgaatg gcttctaaat catctaaaac caacttggat ctcaacaacc ttcacctgct
    13021 caaccttcct gccaggcttt ttatcgccct gctggccgtt gccgccgtgc tggggctcgg
    13081 ttatgccgga ttgttcaaaa gccagatgga atcccttgag gaatacgaag caaaagaaac
    13141 cgaactgaaa aacacctaca aacagaaaag tatcgacgcg gccagcctga acaacctgag
    13201 ggacgaactt gcctcaatcc gctctgcctt cgatatcatg ttgaaacagc tgccgacaga
    13261 tgcagaaatt cccaacctgg ttcaagagct tcatcaggca ggttcgagca acggcctgcg
    13321 cttggacagc gttatgcccc aacctcccgt agatgacggc cccatcaaaa gattacccta
    13381 ttccatttcc attaccggaa attacgaaca gatcagccaa tttacccgcg atgtcggcag
    13441 cctctcccga atcattaccc ttgagtcgct gaaaatcgcc caatctccgg aaaacggcgg
    13501 caatcctgac ggcaagagca gcatcctgaa cctcagtgcc attgccacca cctaccaagc
    13561 aaaatccgta gaagagcttg ccgcagaagc ggcacaaaat gccgagcaaa aataacttac
    13621 gttagggaaa ccatgaaaca ctatgcctta ctcatcagct ttctggctct ctccgcgtgt
    13681 tcccaaggtt ctgaggacct aaacgaatgg atggcacaaa cgcgacgcga agccaaagca
    13741 gaaatcatac ctttccaagc acctaccctg ccggttgcgc cggtatacag cccgccgcag
    13801 cttacagggc cgaacgcatt cgacttccgc cgcatggaaa ccgacaaaaa aggggaaaat
    13861 gcccccgaca ccaagcgtat taaagaaacg ctggaaaaat tcagtttgga aaatatgcgt
    13921 tatgtcggca ttttgaagtc tggacagaaa gtctccggct tcatcgaggc tgaaggttat
    13981 gtctacactg tcggtgtcgg caactatttg ggacaaaact acggtagaat cgaaagcatt
    14041 accgacgaca gcatcgtcct gaacgagctg atagaagaca gcacgggcaa ctgggtttcc
    14101 cgtaaagcag aactgctgtt gaattcttcc gacaaaaaca ccgaacaagc ggcagcacct
    14161 gccgcagaac aaaattaaga agaggattac tccattatga ataccaaact gacaaaaatc
    14221 atttccggtc tctttgtcgc aaccgccgcc tttcagacag catcggcagg aaacattaca
    14281 gacatcaaag tttcctccct gcccaacaaa cagaaaatcg tcaaagtcag ctttgacaaa
    14341 gagattgtca acccgaccgg cttcgtaacc tcctcaccgg cccgcatcgc cttggacttt
    14401 gaacaaaccg gcatttccat ggatcaacag gtactcgaat atgccgatcc cctgttgagc
    14461 aaaatcagtg ccgcacaaaa cagcagccgt gcgcgtttgg ttctgaatct gaacaaaccg
    14521 ggccaataca ataccgaagt acgcgggaac aaagtttgga tattcattaa cgaatcggac
    14581 gataccgtgt ccgcccccgc acgccccgcc gtaaaagccg cgcctgccgc accggcaaaa
    14641 caacaggctg ccgcaccgtc taccaagtcc gcagtatccg tatccaaacc ctttaccccg
    14701 gcaaaacaac aggctgccgc accgtttacc gagtccgtag tatccgtatc cgcaccgttc
    14761 agcccggcaa aacaacaggc ggcggcatca gcaaaacaac agacggcagc accagcaaaa
    14821 caacaggcgg caacaccagc aaaacaaacc aatatcgatt tccgcaaaga cggcaaaaat
    14881 gccggcatta tcgaattggc tgcattgggc tttgccgggc agcccgacat cagccaacag
    14941 cacgaccaca tcatcgttac gctgaaaaac cataccctgc cgaccacgct ccaacgcagt
    15001 ttggatgtgg cagactttaa aacaccggtt caaaaggtta cgctgaaacg cctcaataac
    15061 gacacccagc tgattatcac aacagccggc aactgggaac tcgtcaacaa atccgccgcg
    15121 cccggatact ttaccttcca agtcctgccg aaaaaacaaa acctcgagtc aggcggcgtg
    15181 aataatgcgc ccaaaacctt cacaggccgg aaaatctccc ttgacttcca agatgtcgaa
    15241 atccgcacca tcctgcagat tttggcaaaa gaatccggga tgaacattgt tgccagcgac
    15301 tccgtcaacg gcaaaatgac cctctccctc aaagacgtac cttgggatca ggctttggat
    15361 ttggttatgc aggcacgcaa cctcgatatg cgccagcaag ggaacatcgt caacatcgcg
    15421 ccccgcgacg agctgcttgc caaagacaaa gccttcttac aggcggaaaa agacattgcc
    15481 gatctgggcg cgctgtattc acaaaacttc caattgaaat acaaaaatgt ggaagaattc
    15541 cgcagcatcc tgcgtttgga caatgccgac acaaccggaa accgcaatac gcttgtcagc
    15601 ggcaggggca gcgtgctgat cgatcccgcc accaataccc tgattgttac cgatacccgc
    15661 agcgtcatcg aaaaattccg caaactgatt gacgaattgg acgtacccgc gcaacaagtg
    15721 atgattgagg cgcgtatcgt cgaagcggca gacggcttct cgcgcgattt gggcgttaaa
    15781 ttcggcgcga caggcaagaa aaagctgaaa aatgatacaa gcgcattcgg ctggggggta
    15841 aactccggct tcggcggcga cgataaatgg ggggccgaaa ccaaaatcaa cctgccgatt
    15901 accgctgccg caaacagcat ttcgctggtg cgcgcgattt cctccggtgc cttgaatttg
    15961 gaattgtccg catccgaatc gctttcaaaa accaaaacgc ttgccaatcc gcgcgtgctg
    16021 acccaaaacc gcaaagaggc caaaatcgaa tctggttacg aaattccttt caccgtaacc
    16081 tcaatcgcga acggcggcag cagcacgaac acggaactca aaaaagccgt cttggggctg
    16141 accgttacgc cgaacatcac gcccgacggc caaatcatta tgaccgtcaa aatcaacaag
    16201 gactcgcctg cgcaatgtgc ctccggtaat cagacgattc tgtgtatttc gaccaaaaac
    16261 ctgaatacgc aggctatggt tgaaaacggc ggcacattga ttgtcggcgg tatttatgaa
    16321 gaagacaacg gtaatacgct gaccaaagtc cccctgttgg gcgacatccc cgttatcggc
    16381 aacctcttta aaacacgcgg gaaaaaaacc gaccgccgcg aactgctgat tttcattacc
    16441 ccgaggatta tgggtacggc cggcaacagc ctgcgctatt gatgcgtcaa aataagggca
    16501 tatgttttac ggcatatgcc ctttctttat gctttttgcc gcgaccgaaa tgccgtcatt
    16561 cccgcgcagg cgggaatcca acttgtccgg ttccggttgt ttttcgtctc gtaacttttg
    16621 agccgtcatt cccgcgaaag cggaaatcca gtccgttcgg tttcggtcgt ttccgataaa
    16681 ttcctgctgc ttttcatttc tagattccca ctttcgtggg aatgacggtg gagaggtttt
    16741 tgttgtttcg gagaagtttc tgcaacccta gaatctcgtt atttccacaa aaaacagaaa
    16801 cccgaaacag caacttaaaa cctcgtcatt cccgcaaaag cgggaatccg gtccgttcag
    16861 tttcggtcgt ttccgataaa ttcctgctgc ttttcatttc tagattccca ctttcgtggg
    16921 aatgacggcg gagaggtttt tgttgtttcg gagaagtttc tgcaacccta gaatctcgtt
    16981 atttccacaa aaaacagcaa cccaaaacgg caacttaaaa ccccgtcatt cccgcgcagg
    17041 cgggaatcta ggtctgtcgg ttcaggaact tatcggataa aacggtttct ccaaccctgc
    17101 gttctagatt cccactttcg tgggaatgac gggatatggg tttccgtgcg gacgtgttcg
    17161 gatttccgcc tgcgcgggaa tgacggcgac agatgcccaa cggtctttat agtggattaa
    17221 caaaaatcag gacaaggcga cgaagccgca gacagtacaa atagtacgga accgattcac
    17281 ttggtgtttc agcaccttag agaatcgttc tctttgagcc aaggcgaggc aacgctgtac
    17341 tggtttttgt taatccacta tagtattgat aaacatatta tcttcaatat attcaattgg
    17401 ataattgttt acctaagcaa agataattgc cttttcctga caaataagtg aaatcaacgg
    17461 attgtcaaaa cacagcctga aataaaaaac ctccctgatt tcttttattt ttccttaaaa
    17521 tcagaaaggt tcgggatggt cgggttattt ttccaaacgt accgccgccc tgccgatttc
    17581 gtataaaatt ccgccgtaac ccgacaagcc cgaaccctgt cgccccgaaa ggcggggtgt
    17641 caaacattaa ggaattgtga tgaaaaactt taacggcaaa ctcatcctca tcggactgat
    17701 gggcgcgggc aaaaccacgc tgggccggca aatggcgcag cggctggatt accgttttta
    17761 cgacagcgat cacgaaatcg ccgcagccgc cggcgttccc atccccacca tatttgaaat
    17821 ggaaggcgaa cagggattcc gttcgcgcga aaccgccata ctcaaaaagc tggttatcct
    17881 gccccatatc gtcctgtcca ccggcggcgg cgcggtgtta aaagaagaaa accgcgccct
    17941 tatccgcaaa agcggcacgg tcgtctatct gcacgccccg cccgaaaccc tgctcgaacg
    18001 cacgcgctgc gacaacagcc gtcctttgct gcaagttgcc gatcctttgg cgaaattacg
    18061 tgaactctac gccgcacgcg accccgttta ccgccaaacc gccgacttta ccgtagaatc
    18121 ggcaaactgc cgggaaaccg tgcaaaccct gctcaaacgc ttatcccgat aaaccggcat
    18181 atgcgccgcg cccagaaaac caagccgcgc cccgcccggc gggccggcgg ttcaaacttt
    18241 aaggaacaac aatgaaaaca ctgaccgtac acaccccgtc ccacagctac cccatcttta
    18301 tcggcaacgg actgctgccg caggcaggaa gcctgctcaa accgcatttg ggcaaacgcg
    18361 ccgccatcat cgccaacgaa accgtcgccc cgctctacct cggcacgctt cagacggcat
    18421 tggatgcggc aggcgtatcc catttcagca tcatcctgcc cgacggcgag gcgcacaaaa
    18481 actggcagac gctcaacctc atctttgacg ggctgatgca aaaccgcgcc gaacgcaaaa
    18541 ccacattaat cgcactgggc ggcggcgtga tcggcgacat ggtcggcttt gccgctgcca
    18601 cctaccagcg cggcgcaccg ttcgttcaaa taccgaccac actgttgagt caagtcgact
    18661 catcggtggg cggcaaaacc gccatcaacc acccgctcgg caaaaatatg atcggcgcat
    18721 tctaccagcc gcaggcggtg cttgccgact tggacacgct gcacaccctg cccgtccgcg
    18781 aattgtccgc cggtatggcg gaagtcatca aatacggcgc gctcggcgac atcggttttt
    18841 tcgaatggct cgaacaaaac atatccggac tgatgacgct cgatcgggaa aaactcgccc
    18901 aagccgtgta ccgctgctgc caaatgaagg cagacatcgt cgcccaagac gaaaccgaac
    18961 agggcatacg cgcatggctc aacctcggac acaccttcgg acacgccatt gaaaccgaga
    19021 tgggttacgg cacttggctg catggagaag ccatcgccgc cggctgcgtg ttggcggcgc
    19081 gtttgtccga acaactgggc aaaacctccg ccgcagatac cgcgcggctc gccgccctgc
    19141 tcgaagccgc tggactgccg tccgcaccgc ccgtgtttgc ctttgaaaaa tggctcgaac
    19201 atatgagcca cgataaaaaa gtcagcggcg gcatcatgcg ctttatcggt ctgaaccggc
    19261 tgggcgaagc caacatcacc gaaattaccg acacggacat cctccgccgc accctgcaac
    19321 cgtatctctg atttcctctg ccgatgtgct gccgcgcggg tttgacgcac gatgatgatt
    19381 ttccatcatc tttctccgta aaagcgggaa tccggtccgt tcggtttcgg tcgtttccga
    19441 taagttcccg ttgcttttca tttctagatt cccactttcg caggaatgac ggtggaaagg
    19501 tttttgttgt ttcggagaag tttctgcaac cctagaatct cgttatttcc acaaaaaaca
    19561 gaaacccaaa acagcaactt aaaacctcgt cattcccgca aaagcgggaa tccggtccgt
    19621 tcggtttcgg tcgtttccga taaattcccg ttgcttttca tttctagatt cccactttcg
    19681 tgggaatgac ggcggaaggg ttttggtttt ttccgataaa ttcttgaggc attgaaattc
    19741 cagattcccg cctgcgcggg aatgacagaa ccttaagttt ccgtctttgt tttctgtttt
    19801 cgcgggaata atggggaaca aaccgtattt cagacggcat tttcagttcg gggtataatc
    19861 cgaatacttg cgaccatctg aatcattggg acaaactatg tgtcaactgc tgggcatgaa
    19921 ctgcaatacg ccgaccgata ttatgttttc ctttgaaggc ttccgccggc gcggcggcat
    19981 taccgaccat catgccgacg gtttcggcat cggctttttc gaaggcaaag gcgtgcgcct
    20041 gttccatgac gacaaaccca gtgccaattc gcccgtcgcc gacctcgtgc gcgcctacca
    20101 gattaaatcg gaaaacgtcg tcgcccatat ccgcaaagca tcgcaaggac aaacctcgct
    20161 ggcgaacacc catcccttta tgcgcgaaat gtggggtggc tactggctgt ttgcccacaa
    20221 cgggcatttg attgattttt tccccgaaca gggcgaattt ttccaccccg tcggcacaac
    20281 cgattccgaa cgcgcgttct gccacatcct caaccgcctg cgcacccgct ttgccgcccg
    20341 tcccgacgac gacacgctgt ttgacgcgat tgcggggctg acgcacgaaa tccgcaagtt
    20401 cgggctgttt aactttatgc tttcagacgg cattgccctg tttgcccacg ccagcacact
    20461 gctgcactac atcgtccgcc aagccccgtt cggcaaggcg cgcctgctcg acgacgacgt
    20521 gatggtcgat tttgccgaag taaccacgcc ctccgaccgc gtcgccgtta tcgccaccct
    20581 gccgctgacc cgcgatgaaa cgtgggcgca acttgccgtg gacgaactgg tcatgttccg
    20641 cgaaggcaac atcgtccgac acgaccgtcc cgaaaacccc gtttatatga gtgtcgaaga
    20701 aggtttggaa accgcgcgcg ccgccggcgt cgccgtctga acttcagacg acataggagg
    20761 acgaacccga tgaaatgccc gttttgcgcc caccccgaca cccgcgttgc cgattcgcgc
    20821 ctgatggacg aacgcaacgc cgtccgccgc cgccgatact gccccaactg cggcaaacgc
    20881 ttcggcacgc tcgaaaccgc cgaactcaaa atgcccgccg tcatcggtcc ggacaaaaaa
    20941 cgttcgccct ttaatgcaca acgcctccgc aacgacctga ccgccgccgc ccgaaaatcc
    21001 gccctgacac ccgaacagat cgacgaaacc gtccgcctca cggaactcag gctgtatgct
    21061 tcgggtttgc gcgacatatc cgctgccgaa cttgtcgaaa tcgtactgga agagctgttc
    21121 gggcaaagca cggaagccgc cgtccgtttc gccgccctgc acaaacgctt cgacaatccg
    21181 gcagactttg cctcgtggct ggcgcaagcc gtcaaaacag gcggcaaagc ctgattcccc
    21241 caacccatac tgatacggta tccccatgtt ttcggacaca gatatatcca tgatggaaaa
    21301 cgccctccga cttgccgctt tggggcgttt ttccacttcg cccaatccgc gcgtcggctg
    21361 cgttatcgca caaggcagcc aaattgtcgg acagggcttc cacgtcaaag cgggcgaacc
    21421 gcacgccgaa gtccacgccc tgcgtcaggc gggcgaaatg gcacaaggcg cgaccgcctt
    21481 tgttaccctc gaaccgtgca gccattacgg acgcacaccg ccctgcgccg aagctctgct
    21541 ccgttccggc gtaacacgcg tcgttgccgc catgcgcgac cccaacccgc tggttgcagg
    21601 caaagggctt gccctgctcg aagcagcagg catcaagacg gaatgcggtt tactcgaaca
    21661 tcaggcaagg gaactcaacc gaggcttcct gtcgcgcatc gaacgccgcc gccccttcgt
    21721 ccgcctcaaa tgcgccgttt cgctggacgg caaaaccgcc ctttcagacg gcagcagctt
    21781 ttggattacc ggcgaagacg cgcgtgccga cgtacaggtt ttgcgtgccg aaagctgcgc
    21841 ggtgctgacc ggcatcggca cggtgttggc ggacaatccc cggctcaacg tccgcgcttt
    21901 tccaactttg cgccaacccg cacgcatcgt gttagacagc cgcctgcgcc tgcccttgga
    21961 cagccacctt ctgaccgacg gacaatctcc aacctacatc gccacactcg aacgcaacga
    22021 agacagactg cacccctatc gggaacacgc acacgtccgc atcctgatgc cgtctgaaac
    22081 ggcagacagc aaaatcgacc tgaaccacct gatgcgcctc cttgccgacg aaggtttcgg
    22141 agaaatcatg gtcgaagcag gctccgaact cacatccgca tttctggcag aagatctggc
    22201 ggacgaaatc gtgctgtacc gttcgcccaa aatcctcggc agcggcaaag acctgttttc
    22261 cctgcccgaa aaccgcgccg ccctttccgc accgcccttg tggatgcccg tttccaccga
    22321 aatcctcgga cacgacatca aaaccgtgtt ccggaaaaac gataacgcct tttaaagggt
    22381 ttgcgccgtt tcactatat
//
LOCUS       DAWFHY010000027        22301 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.027, whole genome shotgun sequence.
ACCESSION   DAWFHY010000027 DAWFHY010000000
VERSION     DAWFHY010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.027"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(152..970)
                     /gene="cysE"
                     /locus_tag="WGQ68_000904"
     CDS             complement(152..970)
                     /gene="cysE"
                     /locus_tag="WGQ68_000904"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEZ0714736.1"
                     /translation="MKKDHLNTTGFDLWHTIREETAAAAAAEPMLASFLHQTVLRHES
                     LGSVLAYHLSSKLGSPIMDVRALFEIYQQALGSDTQIGKCVEADLKAIYERDPACDEY
                     SLPLLYFKGFHAIQAHRINHRLYLDGRKTLAYFLQNRMSEVFGVDIHPAARFGYGLML
                     DHATGFVAGETAVLGNNISILHGVTLGGSGKEGGDRHPKIGDGVMIGANASILGNIRI
                     GSNAKIGAGSVVVSDVPPSITVVGVPAKPVARSLKTPSADMDQNIQFTEIDFMI"
     gene            1154..1732
                     /gene="grpE"
                     /locus_tag="WGQ68_000905"
     CDS             1154..1732
                     /gene="grpE"
                     /locus_tag="WGQ68_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEZ0714737.1"
                     /translation="MSEQTQQQNSEEAVENVEAVETVETVGNADGVQEQAAAEPAYED
                     LQARIAELEAQLKDEQLRALANEQNLRRRHQQEIADTHKFAGQKFAVEMLPVKDYLEM
                     ALLDQSGNFDALKMGVQMTLNELQKAFDATQIKEINPKAGDKLDPNIHQAMQAVASEQ
                     EPNTVVGVMKKGYTLSDRVLRPAMVTVAQKEA"
     gene            complement(1856..2071)
                     /gene="nqrM"
                     /locus_tag="WGQ68_000906"
     CDS             complement(1856..2071)
                     /gene="nqrM"
                     /locus_tag="WGQ68_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Na+)-NQR maturation NqrM"
                     /protein_id="HEZ0714738.1"
                     /translation="MKTLLLTFGIFLTVIIGMAVGYIFSKRSIKGSCGGITALGMKKM
                     CDCDTPCDTLQKKLDEEKQAGGIRVDR"
     gene            complement(2097..3152)
                     /locus_tag="WGQ68_000907"
     CDS             complement(2097..3152)
                     /locus_tag="WGQ68_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0714739.1"
                     /translation="MPSETRLPNFIRALIFALGFIFLNACSEQTAQTVTLQGETMGTT
                     YTVKYLSNNRGKLPSPAEIQKRIDDALKEVNRQMSTYQPDSEISRFNQTTAGKPLRIS
                     SDFAYVTAEAVRLNRLTHGALDVTVGPLVNLWGFGPDKSVTREPSPEQIKQAASYTGI
                     DKIILKQGKDYASLSKTHPKAYLDLSSIAKGFGVDKVAGELEKYGIQNYLVEIGGELH
                     GKGKNARGEPWRIGIEQPNIVQGGNTQIIVPLNNRSLATSGDYRIFHVDKNGKRLSHI
                     INPNNKRPISHNLASISVVADSAMTADGLSTGLFVLGETEALKLAEREKLAVFLIVRD
                     KGGYRTAMSSEFEKLLR"
     gene            complement(3304..4521)
                     /gene="nqrF"
                     /locus_tag="WGQ68_000908"
     CDS             complement(3304..4521)
                     /gene="nqrF"
                     /locus_tag="WGQ68_000908"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HEZ0714740.1"
                     /translation="MEIILGIVMFTVIVLVLALMILFAKSKLVSEGDITIKVNGEKEL
                     TMPAGGKLLGALANEGIFIPSACGGGGSCGQCRVVVKSGGGDILPTELSHISKREARE
                     GCRLSCQVNVKTDMDIEVPEEVFGVKKWECTVISNDNKATFIKELKLAIPEGEEVPFR
                     AGGYIQIEAPSHTVAYKDFDIPKEYHEDWDKYNLWQYVSKVDEPILRAYSMASYPEEK
                     GIIMLNVRIATPPPRVPDAPPGQMSSYIWSLKPGDKVTISGPFGEFFAKDTDAEMVFI
                     GGGAGMAPMRSHIFDQLKRLNSKRKITFWYGARSKREMFYVEDFDQLAAEFPNFTWHV
                     ALSDPLPEDNWDGYTGFIHNVVYENHLKNHEAPEDCEFYMCGPPIMNQSVIKMLKDLG
                     VEDENILLDDFGG"
     gene            complement(4535..5128)
                     /gene="nqrE"
                     /locus_tag="WGQ68_000909"
     CDS             complement(4535..5128)
                     /gene="nqrE"
                     /locus_tag="WGQ68_000909"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HEZ0714741.1"
                     /translation="MEHYLSLFIKSVFIENMALSFFLGMCTFLAVSKKVSTAFGLGVA
                     VIFVLGLSVPVNQLVYSLLKDGAIVEGVDLTFLKFITFIGVIAALVQILEMFLDKFVP
                     ALYNALGIYLPLITVNCAIFGAVSFMAQREYNFGESVVYGFGAGLGWMLAIVALAGIT
                     EKMKYSDAPKGLKGLGITFIAAGLMAMAFMSFSGIQL"
     gene            complement(5132..5758)
                     /locus_tag="WGQ68_000910"
     CDS             complement(5132..5758)
                     /locus_tag="WGQ68_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit D"
                     /protein_id="HEZ0714742.1"
                     /translation="MADMKRLKHLMFSPFINNNPIALQVLGICSALAVTTKLQTAIVM
                     GISVALVTGFSSFFISLVRNYIPNSIRIIVQMAIIASLVTLVDQLLQAFAYELSKQLS
                     VFVGLIITNCIVMGRAEAFAMKEPPLESLIDGIGNGAGYGIMLLVVATVRELIGSGKL
                     LGYTVFQTVQDGGWYQTNGLFLLAPSAFFIIGFLIWGLRTWKPEQAEE"
     gene            complement(5758..6534)
                     /locus_tag="WGQ68_000911"
     CDS             complement(5758..6534)
                     /locus_tag="WGQ68_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit C"
                     /protein_id="HEZ0714743.1"
                     /translation="MAKKFDKDSFSGTLIVVLAVSLICSIIVAGAVVGLKPIQEKQKL
                     QDKQGYILSVAGLMDKNTDISKTFAERIEQRVVDLATGEYVADAPKDFSARVAGKDPA
                     QSIQIKPEDDLAGIKSRAKYTEVYLVKGEDGKIGQIILPMHGNGLWSVMYGFVAIQPD
                     GNTINGITYYEQGETPGLGGEIGNPLWQQKFVGKKLFDGQGKLALHVGKGAGSDKEHG
                     VDALSGASLTSKGVQGSFAYWFGENGYIPYLNKLKSAGAQ"
     gene            complement(6527..7759)
                     /locus_tag="WGQ68_000912"
     CDS             complement(6527..7759)
                     /locus_tag="WGQ68_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HEZ0714744.1"
                     /translation="MGLKHFLEKIEPHFLPGGKHEKWYALYEAAATIFYTSGAVTRKA
                     AHVRDALDSKRMMILVWLALFPAMFYGMYNVGAQAFGALTPDLLQQSIANDWHYTLAD
                     ALGINMSSEAGVLGKMLFGAIYFLPIYATVFAVGGFWEVLFATVRKHEINEGFFVTSI
                     LFALIVPPTLPLWQAALGISFGVVVAKEVFGGTGKNFMNPALAGRAFLFFAYPANLSG
                     DAVWTAVDGYSGATALAQWAAHGADGLKNAVTGQTITWMDAFIGKLPGSIGEVSTLAL
                     LIGGAFIVFARIASWRIIAGVMIGMIAMSSLFNFIGSDTNAMFAMPWYWHLVVGGFAI
                     GMLFMATDPVSASFTNVGKWWYGALIGVMCVLIRVVNPAYPEGMMLAILFANLFAPIF
                     DYFVAQANIKRRKARSNG"
     gene            complement(7762..9105)
                     /locus_tag="WGQ68_000913"
     CDS             complement(7762..9105)
                     /locus_tag="WGQ68_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HEZ0714745.1"
                     /translation="MIKIKKGLNLPIAGRPEQVIYDGPVITEVALLGEEYAGMRPSMK
                     VKEGDAVKKGQVLFEDKKNPGVVFTAPVSGKIAAIHRGEKRVLQSVVIAVEGNDEIEF
                     ERYAPEALANLSGEEVRRNLIQSGLWTALRTRPFSKIPAVDAEPFAIFVNAMDTNPLA
                     ADPTVIIKEAAEDFRRGLLVLSRLTERKIHVCKAAGADVPSENAANIETHEFGGPHPA
                     GLSGTHIHFIEPVGANKTVWTINYQDVIAIGRLFATGRLNTERVIALGGSQVNKPRLL
                     RTVLGAKVSQITAGELVDADNRVISGSVLNGAIAQGAHDYLGRYHNQISVIEEGRSKE
                     LLGWVAPQPDKYSITRTTLGHFLKNKLFKFNTAVNGGDRAMVPIGTYERVMPLDILPT
                     LLLRDLIVGDTDSAQALGCLELDEEDLALCSFVCPGKYEYGPLLRKVLETIEKEG"
     gene            complement(9453..10874)
                     /locus_tag="WGQ68_000914"
     CDS             complement(9453..10874)
                     /locus_tag="WGQ68_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEZ0714746.1"
                     /translation="MRHLPLFSLMLFPASVYAADLDGANLNLLWSLPFALILLSIALG
                     PLFFSHTWHHHYGKITAFWTLLFLIPFSLVFGASAGIHTVAHALVEEYIPFILLLLAL
                     YTISGGILVWGDLNGTPKLNTALLAVGTALASIMGTTGAAMLMIRPLLKANQNRTRRV
                     HIVIFFIFLVANIGGGLTPLGDPPLFLGFLKGVDFMWTVKHMFAPVLISTAVLLTAFY
                     FIDNRFFKQESIAQDTPAQQEKPEKIAIFGKWNFLLLSGVVGAVLMSGLWKPEHPGFE
                     ILGSRYALQNLVRDVILIALTAVSMAITPKQVRAGNEFNFEPIAEVGKLFLGIFITIF
                     PVLSILKAGEAGALGGVVSLVHDTAGHPINVMYFWMSGILSAFLDNAPTYLVFFNMAG
                     GDAQALMTGSLFHSLLAVSMGSVFMGALTYIGNAPNFMVKAIAEQRGVPMPTFFGYMM
                     WSVAFLTPVFIVHTLIFFVFKLL"
     gene            complement(11232..11591)
                     /locus_tag="WGQ68_000915"
     CDS             complement(11232..11591)
                     /locus_tag="WGQ68_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEZ0714747.1"
                     /translation="MIRYLLIACGCISLLLGIIGIFLPLLPTTPFVLLSAACWAKASP
                     RFHRWLHRHRYFGPMVHNWEQNGAVPRKAKIFAISMMTASCLIMFWQFPQRWWVGAVS
                     SVFCSLVAIWMWRRPES"
     gene            complement(11588..12193)
                     /locus_tag="WGQ68_000916"
     CDS             complement(11588..12193)
                     /locus_tag="WGQ68_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1415 domain-containing protein"
                     /protein_id="HEZ0714748.1"
                     /translation="MTIMNINTSENKDAVAEHTGQWLEKAVIGLNLCPFAKAPHVKNL
                     VRIAISEAKHLDSFLEDLDEELQRLGNTPATELETTLLVHPTLFPDFDVFNDMLDIAD
                     AAVVENGLEGIIQIAPFHPDFQFEGTDSDDIGNYTNRSPYPTLHLIREDSIAKAAQAF
                     PDASAIFERNIALLEKMGHEGWAKLGITSCPYPHNKKNISK"
     gene            complement(12240..12722)
                     /locus_tag="WGQ68_000917"
     CDS             complement(12240..12722)
                     /locus_tag="WGQ68_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEZ0714749.1"
                     /translation="MPQLTLDKTDIKILQVLQENGRLTNVELSERVALSPSPCLRRLK
                     QLEDAGIVRQYAALLSPESVNLGLQAFIRVSIRKAKDAREDFAASVRKWPEVLSCFAL
                     TGETDYLLQAFFTDMNAFSHFVLDTLLSHHGVQDAQSSFVLKEIKHTTSLPLNHLLKE
                     "
     regulatory      12926..13023
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            13234..14334
                     /gene="gcvT"
                     /locus_tag="WGQ68_000918"
     CDS             13234..14334
                     /gene="gcvT"
                     /locus_tag="WGQ68_000918"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEZ0714750.1"
                     /translation="MITLKTTPFHQAHQDAGAKPVDFAGWELPIHYGSQIAEHEAVRT
                     DAGMFDVSHMLVTDVAGANAKAFFRKLIANDVAKLAFVGKALYSALLNDNGGVIDDLI
                     VYRTNEAETQYRIVSNGATREKDTAQFHKVGQEFGVSFNPRYDLGMLAVQGPKAIEKL
                     LTVKPEWADVVNNLKPFQGADLGNDWFVARTGYTGEDGVEVILPGTEAGVFFKALQAA
                     GVQPCGLGARDTLRMEAGMNLYGNDMDDNTSPLEAGMGWTVDLKDESRDFVGKAALLA
                     LKEKGVAVKQVGLLLGKGGILRAHMEVLTDKGKGETTSGVFSPSLKQSIAIARVPKDF
                     DGDTAKVLIRGKEVDVRVLKLPFVRNGQKQFD"
     gene            14496..14882
                     /gene="gcvH"
                     /locus_tag="WGQ68_000919"
     CDS             14496..14882
                     /gene="gcvH"
                     /locus_tag="WGQ68_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEZ0714751.1"
                     /translation="MSNNIPAELKYVASHEWLRLEEDGTITVGITHHAQELLGDIVFV
                     ELPEVGANLAAEEQAGVVESVKAASDVYAPIAGEVVAVNEDLPSAPETANSDSYGAGW
                     FFKIKPVNPADYDGLLTAEQYAGEVD"
     gene            15029..16276
                     /locus_tag="WGQ68_000920"
     CDS             15029..16276
                     /locus_tag="WGQ68_000920"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEZ0714752.1"
                     /translation="MQLTAVGLNHQTAPLSIREKLAFAAAALPKAVRNLARSNAATEA
                     VILSTCNRTELYCVGDSEEIIRWLADYHSLPIEEIRPYLYALDMQETVRHAFRVACGL
                     DSMVLGEPQILGQIKDAVRVAQEQESMGKKLNALFQKTFSVAKEIRTDTAVGENSVSM
                     ASASVKLAEQIFPDIGDLNVLFIGAGEMIELVATYFAAKSPRLMTVANRTLARAQELC
                     DKLGVNAEPCLLSDLPAILHEYDVVVSSTASQLPIVGKGMVERALKQRQSMPLFMLDL
                     AVPRDIEAEVGDLNDAYLYTVDDMVNIVQSGKEARQKAAAAAETLVSEKVAEFVRQQQ
                     GRQSVPLIRALRDEGEKARKQVLENAMKQLAKGATAEEVLERLSIQLTNKLLHSPTQT
                     LNKAGEEDKDLVHAVAQIYHLDK"
     gene            16552..16977
                     /locus_tag="WGQ68_000921"
                     /pseudo
     CDS             16552..16977
                     /locus_tag="WGQ68_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NosR"
     gene            16983..18212
                     /locus_tag="WGQ68_000922"
                     /pseudo
     CDS             16983..18212
                     /locus_tag="WGQ68_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003777561.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase family maturation
                     protein NosD"
     gene            18270..18890
                     /locus_tag="WGQ68_000923"
     CDS             18270..18890
                     /locus_tag="WGQ68_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714753.1"
                     /translation="MTTHHVELRKVTKRFGAQKAVNQVDLVLKAGESVGLAGHNGAGK
                     STIMKLILGLITPTEGEVMLLGERTGSKAGVRLRSQIGYLPETVALHPSLTGIETLDF
                     YAKLKKQPLTQNRGLLERVGISQAAHRRVGTYSKGMRQRLALAQALLGEPKVLLFDEP
                     TTGLDPASRQMFYEVVRELNGRGATVLLSTHALAELDGHADRIIVD"
     gene            19107..19601
                     /locus_tag="WGQ68_000924"
     CDS             19107..19601
                     /locus_tag="WGQ68_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide reductase accessory protein NosL"
                     /protein_id="HEZ0714754.1"
                     /translation="MKKTLLAIVAVSALSACRQAEEGPPPLPRQISDRSVGHYCSMNL
                     TEHNGPKAQIFLNGKPDQPVWFSTIKQMFGYTKLPEEPKGIRVIYVTDMGNVTDWTNP
                     NADTEWMDAKKAFYVIDSGFIGGMGAEDALPFGNKEQAEKFAKDKGGKVVGFDDMPDT
                     YIFK"
     gene            complement(19689..21350)
                     /locus_tag="WGQ68_000925"
     CDS             complement(19689..21350)
                     /locus_tag="WGQ68_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEZ0714755.1"
                     /translation="MTESITRDSMQYDVVIVGAGPSGLSAAIKLKQLAEQNGREISVC
                     VVEKGSEVGAHSLAGAVIDPIALNELISDWKEKGAPLTRTVTQDKVLFLTEKKAFNLP
                     ITPNFDNHGNYIVSLGEVVRWLAEQAENMGVEIYPGFAAAEVLYHEDGSVKGIATGNM
                     GIGKDGEPTDSFQPGMELWAQQTLFAEGCRGSLSKQIIERFQLDQNSQPQTYGLGIKE
                     VWEVPSEQHQPGLVVHSAGWPLDSKTYGGAFVYHFDDNKVAVGFVVGLDYQNPYLSPF
                     EEFQRFKTHPEIRKTFEGGRRIAYGARSLIEGGLQSLPKLSFKGGILVGDAAGFLNMP
                     RIKGIHTAMKSAMLAAEAVFPLLGNLEEVEGFDSGKEAADYQQRFEQSWLYQELYAAR
                     NVRPSFKWGVYLGSIYTGIDQMIFRGKAPWTLKHHGKDNEQLKKAAACKPIDYPKPDG
                     VLTFDRLSSVFLANLAHEENQPDHLVLNNPQTMIDVNYKEYASPETRYCPAGVYEIVE
                     ENGNPRLQINAANCVHCKTCDIKDPTQNITWICPEGASGPNYGGM"
     gene            complement(21480..22022)
                     /locus_tag="WGQ68_000926"
     CDS             complement(21480..22022)
                     /locus_tag="WGQ68_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0714756.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQKLTEEDVL
                     KKLDKEQMPASFEDMRRIMPDLGFEAKGYALSFEQLAQLQIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKGRDAGGDKAFFT
                     RSPKRQTEFAVGQVKWWRAY"
     gene            complement(22051..22163)
                     /locus_tag="WGQ68_000927"
                     /pseudo
     CDS             complement(22051..22163)
                     /locus_tag="WGQ68_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         5370 a         5559 c         6081 g         5291 t
ORIGIN      
        1 ttccggttgc gtgggaatcg ggtgtatcga ataaaaggca ttttgtccga ctggcaagtg
       61 ccgacatcgg cggcatatca aggcgcaggc ttgaagcggg caatgtcgtc tgaagcccgt
      121 ttggcgtttc agacggcatt ggtgcggata ttcaaatcat aaagtcgatt tcggtaaact
      181 ggatattttg atccatatcc gccgacggtg ttttgagcga tcgcgccacg ggtttggcgg
      241 gtacgccgac aaccgtgatg gacggcggca cgtctgaaac cacgacgctg cccgccccga
      301 ttttggcatt gctgccgatg cggatattgc ccaatatcga ggcgtttgcg ccgatcatca
      361 cgccgtcgcc gattttaggg tggcggtcgc cgccttcttt gcccgaaccg ccgagcgtta
      421 cgccgtgcaa aatcgaaata ttgttgccca acacggcggt ttcgccggca acaaagccgg
      481 tggcgtggtc gagcatcagc ccgtatccga aacgggcggc gggatggatg tccacgccga
      541 atacttcgga catacggttt tgcaggaaat acgccagcgt tttgcgcccg tcgagataca
      601 gccggtggtt gatgcggtgt gcctgaatcg cgtggaagcc tttgaaatat aaaagcggca
      661 gcgaatattc gtcgcaggcg ggatcgcgtt cgtagatggc ttttaagtct gcttcgacgc
      721 atttgccgat ttgggtgtcg ctgcccaacg cctgctggta gatttcaaac agcgcgcgca
      781 cgtccataat cgggctgccg agtttgctgg aaaggtggta ggcaaggacg gagccgaggg
      841 actcgtggcg caacacggtt tggtgcaaaa aacttgccag catcggttcg gcggcggccg
      901 cggccgcggt ttcttcgcgg atggtgtgcc agaggtcgaa accggttgtg tttaaatggt
      961 cttttttcat gagtgatgat gtttgaaaat cgatatggtc ggcagtatct taccgtctat
     1021 attatttttt cggtagggga tttgaaaatg aatttgaaat tctctgcttt tgcttgaagt
     1081 ttcttgaaaa tgtccttatc ttgcgcgggt aataactgga ttttgatttc caatttgttt
     1141 taagggatac gatatgagcg aacagacaca gcagcaaaac agtgaagaag cggttgaaaa
     1201 tgtggaggcg gtggaaaccg tcgagacagt aggaaatgcg gacggtgtgc aggaacaggc
     1261 tgccgcagag ccggcttatg aggatttgca ggcgcggatt gccgagctgg aagcgcagtt
     1321 gaaagacgag cagctgcgcg ctttggcaaa cgagcaaaac ctgcgccgcc gccaccagca
     1381 ggaaattgcg gatacgcaca agttcgccgg acagaagttt gccgtggaaa tgctgccggt
     1441 caaggattat ctggaaatgg cgcttttgga tcagagcggc aatttcgatg cgctgaaaat
     1501 gggcgtgcag atgactttga acgagttgca gaaagcattt gatgctacgc aaatcaagga
     1561 aatcaatcct aaagcgggcg ataagctcga tccgaatatc catcaggcga tgcaggcggt
     1621 ggcaagcgaa caggaaccca ataccgtggt gggcgtgatg aagaagggtt atacgctgtc
     1681 cgaccgcgtg ttgcgcccgg ctatggttac ggtggcacag aaggaagcct gaaggcgtct
     1741 ggggaataat ctgatttatt tcctgaagcg cgttttgcgt ataaaccgat cgaagtaaag
     1801 cggcaatgcc gtctgaatcc gcctgtcggg cttcagacgg cattttgttt cggtgttaac
     1861 ggtcaacccg tatgccgcct gcctgttttt cttcatccag tttcttttgc agggtgtcgc
     1921 aaggtgtgtc gcagtcgcac atttttttca tacccaaggc agtaatgccg ccgcaactgc
     1981 ctttgatgct gcgtttggag aaaatatagc cgaccgccat accgatgatg acggtcagga
     2041 agatgccgaa ggtaaggagc agggttttca tggtgtttcc taatcggttt gtatgtttag
     2101 cggagcagtt tttcaaattc ggaagacatg gcggtgcggt agccgccttt atccctgaca
     2161 atcaggaaaa cagcgagttt ttcgcgctct gccagcttta aggcttcggt ttcgcccaat
     2221 acgaataatc ctgtggacaa gccgtccgcc gtcatcgcac tgtctgcgac cacgctgatg
     2281 gaggcgaggt tgtggctgat gggtcgtttg ttgttcgggt tgatgatatg ggagaggcgt
     2341 ttgccgtttt tatcgacgtg gaaaatacgg taatcgccgg aagtggcaag cgaacggttg
     2401 ttcagcggga cgataatctg cgtattgccg ccttggacga tattgggctg ctcgataccg
     2461 atgcgccacg gttcgccgcg cgcgtttttg cctttgccgt gcaactcgcc gccgatttcg
     2521 accagataat tttgaatgcc gtatttttcc agttcgcccg caactttatc aacgccgaag
     2581 cctttggcaa tcgaagataa atccaaatag gccttggggt gggttttgct caaggaagcg
     2641 taatctttgc cttgtttcaa aatgattttg tctatgcccg tataagatgc cgcctgtttg
     2701 atttgttccg gcgacggttc acgggtaacg gatttgtcgg ggccgaatcc ccaaaggttg
     2761 accaaggggc cgacggttac gtccagtgcg ccgtgcgtca ggcggttcag gcggacggct
     2821 tcggcggtaa cgtatgcgaa gtcacttgaa atgcggaggg gtttgccggc tgtggtttgg
     2881 ttgaaccggc tgatttcaga gtcgggctga taggtggaca tctgccggtt gacttcttta
     2941 agcgcgtcat cgatgcgctt ttgtatttcg gcgggggagg ggagtttgcc ccgattattt
     3001 gaaaggtatt tgacggtata ggtcgtgccc atcgtttcgc cttgcagggt aacggtttgc
     3061 gcggtttgtt ccgaacaggc gtttaggaag ataaaaccca gggcaaatat caaggcgcgg
     3121 ataaagttcg gcaggcgtgt ttcagacggc atagtgtttg atagtcttgg caaatggctt
     3181 gaattatatc gcaaaacggc cggtatgttt ttatgttgat gccgtctgaa aggtgttcgg
     3241 acggtatcgg ataaaaaaag gaagaaaccg aggtttcttc cttttgtgtt tgaagccgaa
     3301 tatttaaccg ccgaaatcgt ccaagaggat gttttcgtct tccacaccca agtctttgag
     3361 cattttgatg acggactggt tcataatcgg cgggccgcac atataaaatt cgcagtcttc
     3421 cggcgcttcg tggtttttca gatggttttc gtaaaccacg ttgtgaatga agcctgtgta
     3481 gccgtcccag ttgtcttccg gcagtggatc ggacagggcg acgtgccacg tgaagttcgg
     3541 gaattctgct gcgagttggt caaagtcttc gacatagaac atctcgcgtt tggaacgcgc
     3601 accataccag aaggtaattt tgcgtttgga gttcaaacgt ttcaactggt cgaaaatgtg
     3661 ggaacgcatc ggagccatac ccgcaccacc gccgataaat accatttcgg catcggtgtc
     3721 tttggcgaaa aattcgccga acgggccgga aatcgtaact ttgtcgccgg gtttgagcga
     3781 ccagatgtag gaagacattt gtcccggagg cgcatcgggt acgcgcgggg gcggcgtggc
     3841 gatacgcacg ttcagcataa tgatgccttt ttcttcagga tacgaagcca tagagtaggc
     3901 gcgcaaaatc ggctcatcca ctttggaaac gtattgccac agattgtatt tgtcccagtc
     3961 ttcgtggtat tccttaggaa tgtcgaagtc tttgtaggca acagtgtgag aaggggcttc
     4021 aatttgaatg tagccgccgg cgcggaaagg aacttcttcg ccttcaggaa tggcaagctt
     4081 gagttcttta atgaacgtgg ctttgttgtc gttggagatg acggtgcatt cccatttttt
     4141 cacgccgaac acttcttcgg ggacttcgat gtccatgtcg gttttgacgt tgacctgaca
     4201 agacagacgg cagccttcgc gtgcttcgcg tttgctgatg tgggacagct cggtcggcag
     4261 gatgtcgccg ccgccgcttt ttacgacgac gcggcattgt ccgcacgaac cgcccccgcc
     4321 gcaggcggag gggataaaga tgccttcgtt ggcaagcgcg cccaagagtt tgccgccggc
     4381 gggcatcgtc agctcttttt cgccgttgac tttgatggtg atgtcgcctt cgctgaccag
     4441 tttggatttg gcaaacagaa tcatcagtgc caaaaccaaa acgatgacgg taaacatcac
     4501 gatacctaaa ataatctcca taccgatccc tttcttataa ctggatgccg gagaacgaca
     4561 taaacgccat cgccatcagg ccggcggcga taaaggtaat gcccaagcct ttgaggcctt
     4621 tgggagcgtc cgaatatttc attttttcgg taatgcccgc caaagcgaca atcgccaaca
     4681 tccagcccaa gcccgcgccg aagccgtata caacggattc gccgaagttg tattcgcgtt
     4741 gcgccataaa cgatacggcg ccgaaaatcg cgcagttcac ggtaatcagc ggcaggtaga
     4801 tgcccaatgc gttatagagg gcggggacga atttatccaa gaacatttcc aaaatctgca
     4861 ccaaagcggc aatcacgccg atgaaggtga tgaatttcaa aaaggtcaaa tccacgcctt
     4921 cgacaatcgc gccgtctttg agcagcgagt aaacgagttg gttgacaggg acggacagcc
     4981 cgagtacgaa aattaccgcc acacccaaac cgaatgcggt ggatactttt ttggatactg
     5041 ccaaaaacgt gcacataccc aaaaagaagg atagtgccat attttcaatg aagacggatt
     5101 tgatgaagag gctcaaatag tgttccatag cttattcctc cgcctgttcg ggtttccagg
     5161 tacgcagtcc ccaaatcaaa aagccgatga tgaagaacgc gctgggggcg agcaggaaca
     5221 agccgttggt ctgataccag ccgccgtcct gcacggtttg gaaaacggtg tagcccaaga
     5281 gtttgcccga gccaatcagt tcgcggacgg tggcgacgac aagcagcatt atcccgtagc
     5341 ccgcgccgtt gccgatgccg tcgatcaggc tttccagcgg cggctctttc atcgcaaatg
     5401 cttcggcgcg gcccatcacg atacagttgg taataatcag accgacgaat acggaaagct
     5461 gtttggacaa ttcgtaggca aatgcctgca agagttggtc gaccagcgta accagcgacg
     5521 cgataatcgc catttgcacg ataatacgga tgctgttggg gatgtagttg cgtaccagcg
     5581 aaatgaagaa gctggaaaaa ccggttacca aagctacgga aatacccatc acgatggccg
     5641 tctgaagttt ggtggtaacc gccaaagccg aacaaatacc caaaacctgc aaggcaatcg
     5701 ggttgttgtt gataaagggt gaaaacatca aatgtttcaa gcgtttcata tcagccatta
     5761 ttgcgctcct gctgatttca atttgttcag gtaggggata tagccgtttt cgccgaacca
     5821 gtaggcgaac gaaccttgca cgcctttgga tgtcagcgat gcaccggaga gggcatctac
     5881 gccgtgttct ttgtccgaac ccgcgccttt gccgacgtgc agggcgagtt tgccttgtcc
     5941 gtcaaacagt tttttgccga cgaatttttg ctgccacaaa ggattgccga tttcgccgcc
     6001 caagcccggg gtttcgcctt gttcgtagta ggtaatgccg ttgatggtgt tgccgtcggg
     6061 ttggatggcg acaaagccgt acatgaccga ccacaaaccg ttgccgtgca tcggcaggat
     6121 gatttggccg attttgccgt cttcgccttt taccaaataa acctcggtgt atttggcacg
     6181 gcttttgatg cctgccaaat cgtcttcagg tttgatttgg atgctttggg cagggtcttt
     6241 gccggcaacg cgtgcgctga agtctttagg cgcatcagcg acatattcgc cggtcgccaa
     6301 atcgacaaca cgttgctcga tacgctcggc aaaggttttg ctaatgtcgg tgtttttatc
     6361 catcaaaccg gctacgctca agatatagcc ttgtttgtct tggagttttt gtttctcttg
     6421 gattggtttc aaaccgacga ccgcaccagc aacgatgatg gaacaaatca ggctgaccgc
     6481 caacacgaca atcagcgtgc cgctgaagct gtctttatcg aatttcttag ccattgctgc
     6541 gcgcctttct gcgtttgatg ttcgcttgtg cgacgaaata gtcgaaaatc ggggcaaaca
     6601 ggttggcaaa cagaatcgcc aacatcatgc cttcggggta agccggattg accacacgga
     6661 tcaatacgca catcacaccg attagcgcgc cgtaccacca tttgccgaca ttggtaaagg
     6721 aagcagaaac ggggtcggtt gccataaaca gcataccgat ggcgaagccg ccgacgacca
     6781 aatgccagta ccaaggcata gcaaacatgg cgttggtgtc cgaaccgatg aagttgaaca
     6841 gcgaagacat cgcaatcata ccgatcatca cgccggcaat aatgcgccaa gaagcgatgc
     6901 gggcaaacac gataaacgcg ccgccgatta agagtgccaa agtggagact tcgccaatgg
     6961 agccgggcag tttgccgata aacgcgtcca tccaagtgat ggtttgaccg gttacggcgt
     7021 ttttcaggcc gtctgcaccg tgtgccgccc attgcgccag cgcggttgcg ccggaatagc
     7081 cgtcaaccgc cgtccaaacc gcatcgccgc tcaagttggc ggggtaggcg aagaacagga
     7141 aggcgcggcc tgccagcgca gggttcataa agtttttacc cgtaccgccg aatacttctt
     7201 tcgcaaccac aacgccgaaa gaaataccca aagccgcctg ccacagcggc agcgtgggcg
     7261 gaacgattaa ggcaaacaga atcgaggtaa caaagaaacc ttcgttgatt tcgtgtttgc
     7321 gtacggtggc gaacaagact tcccagaaac cgccgacggc aaatacggtc gcgtaaatcg
     7381 gcaggaagta aatcgcgccg aacagcattt tgcccaacac gcccgcttca gacgacatat
     7441 tgatgcccaa agcgtcggca agggtgtaat gccagtcgtt ggcgatgctt tgttgcagca
     7501 aatcgggcgt taaggcaccg aatgcctgtg cgccgacgtt gtacatgccg taaaacatgg
     7561 cggggaacaa agccagccac accaaaatca tcatgcgctt ggagtcgagc gcgtcgcgga
     7621 cgtgtgccgc tttgcgcgtt accgcaccgg atgtgtagaa aatcgtcgcc gccgcttcgt
     7681 agagggcata ccatttttca tgtttgccgc ccggcaggaa gtgcggttcg attttttcca
     7741 gaaaatgttt caagcccata atcagccttc cttctcaatg gtttccagca ctttgcgcaa
     7801 cagcgggccg tattcgtatt tgcccgggca gacgaagctg cacaaagcga ggtcttcttc
     7861 gtccaattcc aagcaaccca atgcctgcgc gctgtcggta tcgccgacga ttaaatcgcg
     7921 caaaagcagg gtgggcagga tatccaaggg catcacgcgc tcgtaagtac caatcggcac
     7981 catggcgcgg tcgccgccgt tgacggctgt gttgaacttg aagagtttgt ttttcaggaa
     8041 atggccgagg gttgtacgcg tgatggagta tttgtccggc tgcggcgcaa cccagccgag
     8101 cagctctttg ctgcggcctt cttcgataac ggaaatctga ttgtggtagc gtcccaaata
     8161 atcgtgcgcg ccttgtgcaa tcgcgccgtt caataccgaa ccggaaatca cgcggttgtc
     8221 tgcgtcaacc aattcgcccg cagtaatttg cgatactttc gcacccaaaa cggtacgcaa
     8281 gaggcgtggt ttgttgactt gagaaccacc caaagcaatc acgcgctcgg tgttcagacg
     8341 gcctgttgca aacaaacgtc cgatggcaat tacatcttga taattgatgg tccaaacggt
     8401 tttgttcgca ccgacaggct caatgaaatg aatgtgcgtg ccactcaaac cggcaggatg
     8461 cgggccgcca aattcatgag tttcgatgtt ggcggcgttt tcagacggca cgtctgcgcc
     8521 ggctgcctta caaacatgga ttttgcgttc ggtcaaacgg ctcaatacca gcaaacctcg
     8581 tctgaaatcc tcggcggctt ctttgataat gaccgtaggg tcggcagcca gcggattggt
     8641 gtccatcgca ttgacgaaga tggcgaacgg ctcggcatcg acggcaggga ttttgctgaa
     8701 cggacgggta cgcagcgcag tccacaaacc ggattgaatc agattgcggc gcacttcttc
     8761 gccgcttaag tttgccaacg cttcgggcgc gtagcgttcg aactcgattt cgtcgttgcc
     8821 ttcaacggca atcacgaccg actgaagtac gcgcttttcg ccgcgatgga tggcggcgat
     8881 tttgcctgaa accggcgcgg taaacaccac gcccggattc tttttgtctt caaacagcac
     8941 ttggcctttt ttgacggcat cgccttcctt gactttcatc gaggggcgca taccggcata
     9001 ttcttcgcca agcaacgcga cttcggtaat gacgggcccg tcataaatga cttgctccgg
     9061 tctgcccgcg atgggcaggt ttagaccttt tttgatttta atcatatatt tgcattactt
     9121 gtgatggtta aggtaaaaac ggcgtgtttt gataccgtgt cgcgtggcat caaaagcatt
     9181 gaataaatta atgtagcaaa gtgttagatt ctatcaggaa ttgtacctgt ttgtcagatt
     9241 tgctgctttt ttccttgcgg aagccgtttt tatagtggat taaatttaaa ccagtacagc
     9301 gttgcctcgc cttgccgtac tatttgtact gtctgcggct tcgtcgcctt gtcctgattt
     9361 ttgttaatcc actataattg tcggaagggg ggatattgat ttgattatgc cggaatttaa
     9421 aatgccgtct gaatgttcag acggcatagc gtttacagca gtttgaaaac gaaaaagata
     9481 agggtatgta cgatgaagac gggtgtcagg aaggcgaccg accacatcat atagccgaag
     9541 aaagtcggca tcggtacgcc gcgctgttcg gcaatggcct tgaccatgaa gttcggtgcg
     9601 ttgccgatgt aggtcagtgc gcccatgaat accgaaccca tagaaaccgc cagcagcgaa
     9661 tgaaacaggg aacccgtcat caaggcttgg gcatcgccgc ctgccatatt gaaaaaaacg
     9721 agataagtgg gcgcgttatc caagaatgcc gacaatatgc cgctcatcca aaaatacatc
     9781 acattaatcg gatgacctgc cgtatcgtga accagcgata ccaccccgcc cagcgcgcct
     9841 gcctcgcctg ctttcagaat gctcaggacg ggaaagatgg tgatgaagat gccgaggaag
     9901 agtttgccca cttcggcgat gggttcaaag ttgaattcgt tgcctgcgcg gacttgtttg
     9961 ggcgtgattg ccatagatac ggcggtcaat gcaatcagga tgacatcgcg gacgaggttt
    10021 tgcagggcgt aacggctgcc gaggatttca aatcccgggt gttcgggttt ccaaaggccg
    10081 gacattagaa ccgcgccgac cacgcccgaa agcaggagga agttccattt gccgaagatg
    10141 gcgatttttt cgggtttttc ctgttgtgcc ggcgtatctt gtgcaatgct ttcctgtttg
    10201 aagaaacggt tgtcgatgaa atagaaggcg gtcaacagga cagcggtgct gatcaggacg
    10261 ggggcgaaca tatgtttgac cgtccacatg aaatctacgc ctttgaggaa gccgaggaag
    10321 agtggggggt cgcccaaagg ggtcagaccg ccgccgatgt ttgcaaccag gaaaatgaag
    10381 aagatgacga tgtgcacgcg gcgggtacgg ttttggttgg ctttcagcag cggacgaatc
    10441 atcagcattg ctgcgccggt cgttcccatg atagaggcaa gtgccgtacc gacggcaagc
    10501 agggcggtgt tgagcttggg tgtgccgttc aagtcgcccc aaaccaaaat gccgcctgaa
    10561 atggtgtaca gggcaagcag cagcaggatg aaggggatgt attcttcaac gagtgcgtgt
    10621 gcgacggtat ggataccggc ggacgcgcca aaaaccaaac tgaacgggat gaggaagagc
    10681 aatgtccaaa aggcggtgat tttgccgtaa tggtgatgcc aggtatgcga aaaaaacaag
    10741 ggacccaatg cgatagacag caaaatcagg gcaaagggca ggctccacag caggtttagg
    10801 tttgcgccgt ccaaatctgc ggcgtaaacc gatgcgggga aaagcattag tgaaaacagg
    10861 ggtaggtggc gcatcgtgtt tcctcgattc aagcactgcc ttgcgcggcg cgtgggagtg
    10921 atacaggcac cgtgccgccc ggacataggc ggactgtgct gcctgtttgt ttttgaaaaa
    10981 agttttccga ttgattgtaa agtaaataat caggataaac cagtttccca aaccggaagg
    11041 cggcgggaag gcggattgct gtgcttggga atatatgttc tttttgataa ataattttat
    11101 ttaaaacaaa taaatatatg tctttaataa ataatctatc gaaaacgaaa aatgaattta
    11161 ttttaacata tatttgcaat gaaacaggtt tgccccccgt ttgtttgccc ttatcccttt
    11221 cagtacggca ttcaagattc gggcctgcgc cacatccata tggcgacaag ggaacaaaaa
    11281 accgatgaaa ccgccccgac ccaccagcgt tggggaaact gccaaaacat tatcaggcag
    11341 gatgcggtca tcatactgat ggcgaatatt ttggctttgc gcggcactgc gccgttttgt
    11401 tcccagttat gaaccatcgg accgaaatag cggtgccggt gcagccagcg gtgaaagcgc
    11461 ggggatgcct ttgcccagca ggcggcggag agcagtacga acggcgtggt cggcaacagc
    11521 ggcaaaaaaa tgccgatgat acccaacagc agggaaatgc agccgcaggc aattaaaaga
    11581 taacgtatca ttttgaaata tttttcttat tgtgcggata agggcaggat gtgataccga
    11641 gttttgccca gccctcatgt cccatttttt ccagcagggc gatattgcgt tcgaatattg
    11701 ccgaagcgtc gggaaaggct tgtgcggctt tggcaatgct gtcttcgcgg atgaggtgca
    11761 gcgtcggata gggagaacgg ttggtgtagt tgccgatgtc gtctgaatcc gtgccttcaa
    11821 attggaaatc gggatgaaac ggggcgattt ggatgatgcc ttccaagccg ttttcgacaa
    11881 cggcggcatc ggcaatgtcg agcatatcgt tgaatacgtc gaaatcgggg aatagggtcg
    11941 ggtgaaccag cagggtagtt tccagttcgg tggcgggtgt attgcccagt cgctgcagtt
    12001 cttcgtccaa gtcttccaaa aaactgtcaa ggtgtttggc ttcgctgatt gcgatgcgga
    12061 caaggttttt aacgtggggg gctttggcaa agggacacag gttcagaccg atgacggctt
    12121 tttccaacca ttgtccggtg tgttcggcaa cagcatcttt attttcggaa gtattgatat
    12181 tcattattgt catgtaaatg tgtttgcaga ttgcacgtgc gggaaaatcg ggaagggcac
    12241 tattccttca gcaggtggtt gagcggcagg gaggtggtgt gtttgatttc ttttaaaaca
    12301 aagctcgatt gtgcatcttg tacgccgtgg tgggacagga gcgtatccaa aacaaaatgg
    12361 gaaaacgcgt tcatatcggt aaaaaacgcc tgaagcaggt agtcggtttc ccctgtcagg
    12421 gcgaagcagc tcaagacttc cggccatttt cggaccgatg cggcaaagtc ttcccgcgcg
    12481 tcttttgctt tgcggatgga aacgcggata aatgcctgaa gccccaggtt gacggattcc
    12541 ggagacagca gcgcggcata ttggcggacg atgccggcat cttccaactg ctttagacgg
    12601 cgcaggcacg gagaaggcga aagtgcgaca cgttcggaca gttcgacatt ggtcagcctg
    12661 ccgttttcct ggagaacctg taagatttta atatcggttt tgtctaaagt gagttggggc
    12721 atatttgcgt tccgttttaa ggaattcgga ttgtctgtcc gtatgtttgc ggcaatccgc
    12781 acagatggag accatattaa catataaaaa gttataccgt catccgggac aaattttgtt
    12841 ttcggaaaat catgtgaaaa cagaggcggt cggtttgcat ctcttttaag acggcttgcc
    12901 caaaccgccg attcaagaca taatcgggaa atgtgcagga gagtgttaca cccaactaca
    12961 atgtaaccac cgaaggcgca gacaccctta aatcgctcag gtatcaggga ctgcacattg
    13021 aaacaaacaa tctggagagc ggcgttggaa taacgtccac cgaaggggag aaggccgtct
    13081 gaaccaccat tcagacaacc gcgcaaagca gtgagcagac tggtttgcca tcatgcggat
    13141 acggccgaaa atctcaggtt caaggacaga tagggtcatc cgcgcacagg tgcgcgggtg
    13201 gcatctgaac aaaaaatccg gagaaacttg agaatgatta ctctgaaaac caccccattt
    13261 catcaagccc atcaagatgc aggcgcgaag ccggtagatt ttgccggctg ggagctgccc
    13321 atccattatg gttcgcaaat cgccgaacac gaagccgtgc gcaccgacgc cggtatgttt
    13381 gacgtatccc atatgcttgt taccgacgta gcaggcgcaa atgccaaagc ctttttccgc
    13441 aaattgattg ccaacgatgt cgccaagctc gcttttgtcg gcaaagccct ttattctgct
    13501 ttgctcaacg acaacggcgg cgtgattgac gacttgatcg tttaccgcac caacgaggcc
    13561 gaaacccaat accgcatcgt gtccaacggc gcgactcgcg aaaaagatac ggcacaattc
    13621 cacaaagtcg gacaagagtt cggcgtttct ttcaatccgc gctacgacct cggcatgctc
    13681 gccgtacaag gcccgaaagc catcgagaaa ctcctgaccg tcaaacccga atgggcagat
    13741 gtcgtcaaca acctcaaacc gttccaaggc gcggatttgg gcaacgactg gtttgtcgcc
    13801 cgcaccggct ataccggcga agacggcgtc gaagtgattc tgccgggtac tgaagcaggc
    13861 gtattcttca aagctctgca agcagcaggc gtacagcctt gcggcctcgg cgcacgcgac
    13921 accctgcgca tggaagccgg tatgaacctc tacggcaacg atatggacga caataccagt
    13981 ccgctcgaag caggcatggg ttggaccgtt gatttgaaag acgaaagccg cgattttgtc
    14041 ggcaaagctg ccttgttggc attgaaagaa aaaggcgttg ccgtcaaaca ggtcggtttg
    14101 ctgctcggca aaggcggcat cctgcgcgcg catatggaag tgttgaccga caaaggcaaa
    14161 ggcgaaacca ccagcggcgt attctcgccc agcctgaaac aatccatcgc catcgcccgc
    14221 gtgccgaaag actttgacgg cgataccgcc aaagttctga ttcgcggcaa agaagtggac
    14281 gtacgcgtac tgaagctgcc gtttgtgcgc aacggacaga aacagtttga ttaatatcgg
    14341 ttcagacggc attttcattt catatgccgt ctgaaagcag gttttaattg ttgtccgata
    14401 cggacgtttg tagaaagcat tgaacaaggc atctgtggat attgattcat gcagatgccg
    14461 tctgaaaata acccctatca atggagtatc aaaccatgag caacaacatc ccggccgaac
    14521 tgaaatacgt tgccagccat gaatggctgc gccttgaaga agacggtacg attaccgtcg
    14581 gcattaccca ccacgcgcaa gagctgttgg gcgacatcgt gttcgtcgag ctgcccgaag
    14641 tcggcgcgaa ccttgccgct gaagagcaag ccggtgtggt tgagtctgta aaagccgcgt
    14701 ccgacgtgta cgcaccgatt gcaggcgaag tcgttgccgt caacgaagat ttgccaagtg
    14761 ctccggaaac tgccaacagc gattcttacg gcgcaggctg gttcttcaaa atcaaacctg
    14821 tcaatcctgc cgattacgac ggtctgctga ctgccgaaca atacgcgggc gaagtggact
    14881 aaatctctat cctgataatg ggatgactga tttgagaaac tgcactttca gacggcaagg
    14941 atgcgtttgg aagtgcagtt tttgtctgtg tcttcaaagt tcagttaggc tataataacg
    15001 gtctatattc atctttaccg attttttcat gcaacttacc gctgtcggac tcaatcatca
    15061 aaccgcacct ttaagcatac gggaaaagct ggcgtttgcc gccgccgccc tgcctaaagc
    15121 cgtccgcaat cttgcccgaa gcaatgcggc aacggaggcg gtaatccttt ctacctgcaa
    15181 ccgcaccgag ctttactgcg tcggtgattc ggaagaaatc atccgatggc ttgccgatta
    15241 ccacagtttg ccgattgaag aaatccgtcc gtatctgtac gcgctggata tgcaggaaac
    15301 cgtgcgccat gccttccgcg ttgcctgcgg cttggattcg atggtgttgg gcgagccgca
    15361 gattttagga cagattaagg atgcggtcag ggttgctcaa gagcaggaaa gtatgggtaa
    15421 gaaactcaat gccctgttcc aaaaaacctt ttccgttgct aaagagatcc gtaccgatac
    15481 cgctgtcggc gagaactcgg tttccatggc ttccgcttcc gtcaagttgg cagagcagat
    15541 tttccccgac atcggcgatt tgaatgtctt gtttatcggt gcgggtgaga tgattgagct
    15601 ggttgccact tattttgccg ccaaaagtcc ccggctgatg acggttgcca accggacgct
    15661 ggcgcgtgca caggagttgt gcgacaagct cggtgtcaac gccgaaccgt gcctgctgtc
    15721 cgatctgcct gccattttgc atgagtacga cgtggtggtt tcttcaacgg caagccagtt
    15781 gcccattgtc ggcaaaggta tggtggagcg cgcattgaaa caaaggcaga gtatgccgtt
    15841 gtttatgctt gacttggccg tgccgcgaga cattgaggcg gaagtcggag atttgaacga
    15901 tgcctatctt tatacggtgg acgatatggt caatatcgtc caaagcggca aggaggcaag
    15961 gcagaaggcc gccgccgccg ccgaaacgct ggtgtccgag aaggttgccg aatttgtcag
    16021 gcagcagcag ggcaggcaga gtgtcccgtt aatcagggca ttgagggatg agggagagaa
    16081 agcgcgcaaa caggtcttgg aaaatgcgat gaaacagctt gccaaaggcg caacggcaga
    16141 agaggttttg gaaaggctgt cgatccaact gaccaacaag ctgctgcatt cgccgaccca
    16201 aactttgaat aaggcggggg aagaagataa agatttggtt catgccgtcg cgcagattta
    16261 tcatttggac aaataacggt gcgccgggaa aaatgccgtc tgaagaggtt tcagacggca
    16321 tttttttgtg ccgcctgaca acatcgtgaa atcccacatt atatcgatgt aatcacaaag
    16381 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta cagatagtac
    16441 ggcaaggcga ggcaacgctg tactggttta aatttaatcc actatattat cccgtatgcg
    16501 gattggtttt aagatttgta aatttaattt gcatcaaaaa atcgccgata gatgattcat
    16561 ataatatcaa tattaaagag tatcggtata tcggggatag tcatgtcctg tttttcaatc
    16621 aaacgtatgt ccgcgtttcg ggcgcggata acggcgtttt ttgccgcctt tgtctttttg
    16681 acggcggcac tgcccgctta tgcggagcgt ctgcctgatt ttctggcgaa aatacagcct
    16741 tcggaaattt ttccgggtgc ggatcgttac ggcaagccgg aaggtaagcc tatggttgcc
    16801 cgcgtttaca aaggcgatga gcagttgggc ttggtctata tcacgaccga tgcggtcaat
    16861 acgcgcggtt attcgagcaa gccgattgat acgctgatgg cgctggcaaa cgacggcacg
    16921 atagccgggg cgaaattggt tgatcaccat gagtcgatta tgctgatcgg tatcccgcat
    16981 ttgcccgcgc ccgggcgggc gatacgctca aactggcttc cggcgtatat caaaccaaac
    17041 ttcacatcga caaaccgatt acgattgaag ggcctgccga ccgttctgca accatcgaag
    17101 gggacaggag cgggcgtacc atagccgtac acgcgccgga cgtaacgctc cgcaacctga
    17161 ccgttacccg ttccggcatg agcctgccag caatggatgc cggtatttat ctcgaagaaa
    17221 ccgctccgca cgccctgatt gaacacaata atattttgga taattcggtc ggcgtctatc
    17281 tgcatggttc ttccgaagcg atggtgcgcg agaacaaaat cgtcggcgac gcgactttgc
    17341 gcgtgaacga gcgcggcaac ggcgttaccg tttggaacgc gcccggtgcg caagtcgtcg
    17401 gcaacgacat ttccaaaggg cgggacggca ttttttccaa caccagcaca tataacacct
    17461 acaaaaacaa ccgcttcagc gatttgcgtt tcgccgtcca ctatatgtac accaacgaca
    17521 gcgaaatcag cggcaatatt tccgtgggca acaatatggg ctatgtgctg atgttttccg
    17581 agcggctcaa agtattcgac aatatcgccg tcggcagccg cgatcagggc attatgctca
    17641 actatgtcaa ctattccgat attcacgaca acattatcaa caaagcgggc aagtgcgtat
    17701 ttgcctataa tgccaactac gataaacttt tcgccaatca ttttgaaaac tgccaaatcg
    17761 gcatacactt taccgccgcc atcgaaggca cgtccctgca cgacaattcc ttcatcaaca
    17821 acgaaagcca ggtcaaatac gtcagcacgc gctttctcga ctggagcgag ggcggacacg
    17881 gcaactattg gagcgacaac agcgcgttcg atttgaacgg cgacggcttc ggcgacagcg
    17941 cgtaccggcc caacggcatc atcgaccaaa tcatctggcg cgcgcccgta tcgcgtctct
    18001 tgatgaacag tcccgcaatc agcatcgtca aatgggcgca ggcgcagttt cccgccgttc
    18061 tgcctggcgg cgtggtggac agcaaaccgc tgatgaagcc ttatgccccc aaaattcaaa
    18121 cccgttatca ggcgatgaag gacgagttgc tcaaagaagc cgaaacgcgg cagtcggaat
    18181 ggggcagggc ggagaacggt tctttgaact agtctgcttc agacggcatc cggatccaaa
    18241 tgccgtctga aaacacaaaa ggaacaacca tgaccacaca tcatgtcgaa ttgaggaagg
    18301 taaccaaacg gtttggggcg caaaaagccg tcaaccaagt cgatttggtt ttgaaggcag
    18361 gagaaagcgt cgggcttgcc ggacacaacg gcgcgggcaa gtccaccatt atgaagctga
    18421 tactcgggct gattaccccg accgaaggcg aagtgatgct tttgggcgaa cgtaccggta
    18481 gcaaagcggg ggtgcggctt cgcagccaaa tcggctacct gcccgaaacc gttgcgctgc
    18541 acccttcgct gaccggcatc gaaacgctgg atttttatgc caaacttaaa aaacagccgc
    18601 tcacgcagaa ccgggggctg cttgagcgtg tcggtatttc acaggcggca caccgccgcg
    18661 tcggcaccta ctcgaaaggg atgcgccaac gccttgcctt ggcacaagcc ctgctgggcg
    18721 aacccaaagt cctgctgttt gacgaaccga caaccggtct tgaccctgca tcaaggcaaa
    18781 tgttttacga agtcgtgcgc gaactcaacg ggcgcggcgc gaccgtattg ctcagcaccc
    18841 acgcccttgc cgagttggac ggacacgccg accgcattat cgtggattaa atttaaacca
    18901 gtacggcgtt acctcgcctt gccgtactat ttgtactgtc tgcggcttcg ttgccttgtc
    18961 ctgatttttg ttaatccact atatgtgggt atggcgggtt tgagcggaca aatcagcctg
    19021 accgtccccg ttttgctgac cgctcaggtt ttatgggtta tcattccgct tgttttggca
    19081 gccggaattt ttagaaagcg acaaatatga aaaaaaccct gttggcaatt gttgccgttt
    19141 ccgccttaag tgcctgccgg caggcggaag agggaccgcc gcctttaccc cggcagatta
    19201 gcgaccgttc ggtcggacac tattgcagta tgaacctgac cgaacacaac ggccccaaag
    19261 cccagatttt cttgaacggc aaacccgatc agcccgtttg gttctccacc atcaagcaga
    19321 tgttcggcta taccaagctg cccgaagagc ctaaaggcat ccgcgtgatt tacgttaccg
    19381 atatgggcaa tgttaccgat tggacgaatc ccaatgccga cacggagtgg atggatgcga
    19441 aaaaagcctt ttacgtcatc gacagcggct ttatcggcgg tatgggtgcg gaagacgcgc
    19501 tgccgttcgg caacaaagag caggctgaga aatttgcaaa ggataaaggc ggtaaggttg
    19561 tcggtttcga cgatatgcct gatacctata ttttcaaata atattgaaaa aaccggccga
    19621 aactttaaaa acgagtttcg gtcggttttt cttttcttgt tcggtatagt gtcggcagga
    19681 aagaaccttc acatcccgcc gtaattcggc ccgctcgcgc cttcggggca aatccaagtg
    19741 atgttttgcg tcgggtcttt gatgtcgcag gttttgcagt gcacgcagtt tgccgcgttg
    19801 atttgcaggc gcggattgcc gttttcttca acaatttcgt acacgccggc cggacaatag
    19861 cgcgtttcgg gcgaggcgta ttctttgtag ttcacgtcta tcatcgtttg cggattgttc
    19921 agcaccaaat ggtcgggctg gttttcttcg tgcgcgagat tggcaaggaa gacgctgctc
    19981 aagcggtcga aggtcaacac gccgtcgggt ttcggataat caatcggctt acacgcggcg
    20041 gcttttttaa gctgctcgtt gtctttgccg tgatgtttca aggtccacgg ggctttgcct
    20101 ctgaaaatca tctgatcgat gccggtgtag attgagccga ggtaaacgcc ccatttgaat
    20161 gacggacgga cattgcgcgc ggcgtaaagc tcttgataca gccagctttg ttcaaaacgt
    20221 tgctgataat ccgccgcctc tttgccgctg tcgaaaccct ccacttcttc aaggtttccc
    20281 aacaagggga acacggcttc ggcggcgagc atggcggatt tcatcgcggt atgaatgcct
    20341 ttgatgcgcg gcatattgag gaaacccgcc gcatcgccga ccaaaatgcc gcctttgaac
    20401 gagagcttcg gcaaactttg caaaccgcct tcaatcagcg aacgcgcgcc gtaagcaatg
    20461 cggcggccgc cttcaaaggt tttgcggatt tcgggatggg ttttgaaacg ttggaactct
    20521 tcaaacggcg acagataagg attttgatag tccaaaccga ccacgaagcc gacggcgact
    20581 ttgttgtcgt cgaaatggta aacaaacgcg ccgccgtagg ttttgctgtc cagcggccag
    20641 cctgcgctgt gcaccaccaa accgggctga tgctgttcgg acggcacttc ccaaacttct
    20701 ttaatgccca agccgtaagt ttgcggctgg ctgttttggt cgagttggaa acgttcgatg
    20761 atttgtttgg aaagcgaacc gcgacaacct tcggcaaaca gggtttgctg cgcccaaagc
    20821 tccatgccgg gttggaatga atcggtcggc tcgccgtctt tgccaatgcc catattgccg
    20881 gttgcaatgc ctttgaccga accgtcttcg tgatacagca cttcggcggc ggcaaagccc
    20941 ggatagattt ccacgcccat attttccgcc tgctccgcca accagcgcac gacttcgccc
    21001 aagctgacga tgtagttgcc gtgattgtcg aaattcgggg taatcggcag gttgaacgct
    21061 tttttctcgg tcaggaacaa caccttgtcc tgcgttactg tgcgtgtcag cggtgcgcct
    21121 ttttctttcc agtcggaaat caactcattc agcgcaatcg gatcgataac tgcgccagcc
    21181 agcgaatgcg cccccacctc cgaacctttc tccaccacgc aaacgctgat ttcgcgcccg
    21241 ttttgttcgg caagctgctt gagtttgatg gcggcagaca aacccgacgg gcctgcgccg
    21301 acaatcacga catcgtattg catactgtcg cgggtgatgg attctgtcat ggcggttcct
    21361 gtgtatttat tattgaattg caaatccgta attatacaac gggaacatat agttaccaaa
    21421 tacaacaaag gtcgtctgaa aaccatattt tcggttttca gacgaccttt gtcgaaattt
    21481 caataagcac gccaccattt tacctgtccg accgcaaact ccgtctgacg tttcggactg
    21541 cgtgtgaaaa acgccttatc cccgccggca tccctccctt tcggcacaac cgccaaaatc
    21601 ttacctgcca aatttccctc acgggtttgc caagcatcca aaaactgcgc cctgctcatc
    21661 gaaacatgac ccagcgacgg gtcggcaagc aaaaccgtat tgccgtctat accgcgcaat
    21721 accgagaaat gatcatcctt gcggtatttc agatacacga tgacggggat ttgcaactgt
    21781 gcaagctgct cgaaagacag ggcatagcct ttcgcttcaa aacccaaatc aggcataatg
    21841 cgccgcatat cctcaaacga cgcgggcatc tgctccttat ccagtttttt taacacgtcc
    21901 tcttccgtca gcttttgccc gtaaaaattg ttcaaaagcg tcgccaccga agccgccccg
    21961 caggaaaaat ccaaatcctg ctttacaata ttgaaatcgc gcctctcttt ccaactctgc
    22021 actttgattt ttccataagc aacaggatta tagtggatta aatttaaacc agtacggcgt
    22081 tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt
    22141 gttaatccac tataggtttc cgtgcggacg tgttcagatt cccgctttcg ctggaataac
    22201 ggcggagcag tttctgcttt tccgataaat gaccgtaact taaaatcccg tcatccccac
    22261 gaaagcaaaa atcctgcctg tcggatttcg gtttttttgg g
//
LOCUS       DAWFHY010000028        22232 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.028, whole genome shotgun sequence.
ACCESSION   DAWFHY010000028 DAWFHY010000000
VERSION     DAWFHY010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 22232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22232
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.028"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(120..1034)
                     /locus_tag="WGQ68_000928"
     CDS             complement(120..1034)
                     /locus_tag="WGQ68_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0714757.1"
                     /translation="MDAVQLKSFVAVAHEGNLTQAAKRLFLSQPAVSAQIKALEEYVG
                     TPLFRRTGKGMVLTRAGEILLPEAESLLQYKHKLEYFAKTLAGDYSEETSLGIIHPID
                     SAKLVALTDNIGQTAPKTRLHIQYGMSGEILSRIQHKTLHGGFILGNAAQRGIRSVFL
                     QNLTYALICPQSQYPHLTRSLPQSLQECVWIEMSGVSGSRKHLHQFWRSNRLSPKKQI
                     LCDYPQTIIDLVAGGIGVAMVPGNKAEAAAKEGAGVAIIESCRHSMPLNFIYAEEYED
                     NPHVSLLLECIEKVWGVQAVQPPVVSDN"
     gene            1290..2759
                     /locus_tag="WGQ68_000929"
     CDS             1290..2759
                     /locus_tag="WGQ68_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3360 family protein"
                     /protein_id="HEZ0714758.1"
                     /translation="MEKHNGTYRDLHRPASEFATRDEYLEHELQIMQPKRWRPNLPFR
                     DYRFEWEDLIPAMAGTIGKVVMVGAVAAAFAAPLGLPDSFVLENVRYELLIAAAFILL
                     VSGFFLPGANLPGTHGPLIPMIPIVVSAGGHPLAFGISIAVLGLLMALFRGGSIMAKL
                     TSNGVCGGLLLYLGFIGTTGQVKKLFSWASGFNMPYIAFTVIIVTIVMYALLEHWKKR
                     WLAVPLGCLIAGVVAFALGAPFEFHTAPGLPPMSPAYWWGENSGWHLGLPTAESFLVV
                     FPFAVLAVAMWSPDFLGHQVFQKISYPEKTDKVLMNIDDTMTSCSVRQAVGSILGGAN
                     FTSSWGTYIVPASIAKRPIPGGAVLTAVLCIIAGLWGYPMDLAIWQPVLSVALVVGVY
                     LPLLEAGMEMTRKGKTTQSAAIVVFSSALVNPVFGWALTMLLDNLGLIGCKERSAQLG
                     FAGRVLIPAVGFLILCVAMGAVGMLPGIPPFLEHFKSLG"
     gene            3011..3382
                     /locus_tag="WGQ68_000930"
     CDS             3011..3382
                     /locus_tag="WGQ68_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714759.1"
                     /translation="MNKTLSILPVAILLGGCAAGGGNTFGSLDGGTGMGGSIVKMAVG
                     SQCRAELDKRSEWRLTALAMSAEKQAEWENKICACVAQEAPERMTGNDVMQMLAPSTR
                     NQALAALTAKTVSACFKHLYR"
     gene            3455..4864
                     /locus_tag="WGQ68_000931"
     CDS             3455..4864
                     /locus_tag="WGQ68_000931"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEZ0714760.1"
                     /translation="MKYISTRGETAHKPFSEVLLMGLAPDGGLMLPEHYPQIGRETLD
                     KWRGLAYPELAFEIMRLFVTDIPEDDLRDILNRTYTEAAFGTKEITPVRTLSDGIKIQ
                     ALSNGPTLAFKDMAMQFLGNAFEYVLNKEGKKLNILGATSGDTGSAAEYALRGKKGVN
                     VFMLSPDGKMSAFQRAQMYSLQDGNIHNIAVKGMFDDCQDIVKAVQNDAAFKEKYHIG
                     TVNSINWGRIVAQVVYYFAGYFNATSSNDETVSFCVPSGNFGNVCAGHIAKQMGLPVR
                     RLIVATNENDVLDEFFKTGAYRPRNSAHTYVTSSPSMDISKASNFERFVFDLMDRDPQ
                     EINTLWAEVAAGKGFDLRFALEKVGGKYGFTSGKSTHPDRLATIRQVYEQDQELIDPH
                     TADGVKVAREVREEGETVVCLETALAAKFDATIREAVGDAAIPRPAALEGLENLPQRV
                     QTVPNSADAVKGIIEQTLA"
     gene            4910..5704
                     /locus_tag="WGQ68_000932"
     CDS             4910..5704
                     /locus_tag="WGQ68_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714761.1"
                     /translation="MTLPMQETRFSILLDELAAKQEAAIAPHLLADGTKVWVRKAGRH
                     NARWRYALLGMVARYLKLGVLKPVPSLGGEPAITTESKRLYELRSAGIAVPELLAVRK
                     NALMFGNLAGIPLDTQIRQEAEAGKADAWLAGLEAIARVHKKQQFLSQAFARNMMSDG
                     ENISFLDFEDDPSEVLTIAQCQARDWLCYIHSTALILKNGGLLEAAAEKWGGVLSDQP
                     AEIQKLIAATVKPILPIRRLEHPRWGRDALRLAASISLISLADIPP"
     gene            5892..6668
                     /locus_tag="WGQ68_000933"
     CDS             5892..6668
                     /locus_tag="WGQ68_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0714762.1"
                     /translation="MAAFNTQKVLSVHHWTDAYFTFTCTRDESLRFENGQFVMVGLMV
                     DGKPLMRAYSVASANWEEHLEFFSIKVQDGPLTSRLQHLKVGDDVLISKKPTGTLVAG
                     DLNPGKHLYLLSTGTGIAPFLSITKDPEIYEQFEKIILVHGVRYKKDLAYYDRFTKEL
                     PEHEYLGDLVKEKLIYYPIVSREEFEHHGRLTDLMVSGKLFEDIGLPKINPQDDRAML
                     CGSPAMLKDTCKVLDDFGLTVSPKTGVRGDYLIERAFVDQ"
     gene            complement(7308..9797)
                     /locus_tag="WGQ68_000934"
     CDS             complement(7308..9797)
                     /locus_tag="WGQ68_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEZ0714763.1"
                     /translation="MKKTCFHCGLDVPENLHLTVRYEGEDRETCCAGCQAVAQSIIDA
                     GLGSYYKQRTADAQKTELPPQEILDQIRLYDLPEVQSDFVETHGGTREAVLMLGGITC
                     AACVWLIEQQLLRTDGIVRIDLNYSTHRCRVVWDDGKIRLSDILLKIRQIGYTAAPYD
                     AQKIEAANQKERKQYIVRLAVAGLGMMQTMMFALPTYLYGGDIEPDFLQILHWGGFLM
                     VLPVVFYCAVPFYQGALRDLKNRRVGMDTPITVAIIMTFIAGVYSLATNAGQGMYFES
                     IAMLLFFLLGGRFMEHIARRKAGDAAERLVKLIPAFCHHMPDYPDTQETCEAAVVKLK
                     AGDIVLVKPGETIPVDGTVLEGSSAVNESMLTGESLPVAKMPSEKVTAGTLNTQSPLI
                     IRTDRTGGGTRLSHIVRLLDRALAQKPRTAELAEQYASSFIFGELLLAVPVFIGWTLY
                     ADAHTALWITVALLVITCPCALSLATPTALAASTGTLAREGILIGGKQAIETLAQTTD
                     IIFDKTGTLTQGKPAVRRISLLRGTDEAFVLAVAQALEQQSEHPLARAILNCRISDGS
                     VPDIAIKQRLNRIGEGVGAQLTVNGETQVWALGRASYVAEISGKEPQTEGGGSAVYLG
                     SQSGFQAVFYLTDPLKDSAAEAVRQLAGKNLTLHILSGDRETAVAETARALGVAHYRA
                     QAMPEDKLEYVKALQKEGKKVLMIGDGINDAPVLAQADVSAAAAGGTDIARDGADIVL
                     LNEDLNIVPHMFAQARRTQQIIRQNLIWAGAYNIIAVPLAVLGYVQPWIAALGMSFSS
                     LAVLGNALRLHKRGKMPSEKMPSEQCRTALL"
     gene            complement(9794..10972)
                     /gene="hflX"
                     /locus_tag="WGQ68_000935"
     CDS             complement(9794..10972)
                     /gene="hflX"
                     /locus_tag="WGQ68_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009347253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEZ0714764.1"
                     /translation="MTGRTGRNGSTQAQPERVMLVGVMLDKDGTGSSAARLNGFQTAL
                     AEAVELVKAAGGDSVRVETAKRDRPHTALFVGTGKAAELSEAVAADGIDLVVFNHELT
                     PTQERNLEKELKCRVLDRVGLILAIFARRARTQEGRLQVELAQLSHLAGRLIRGYGHL
                     QSQRGGIGMKGPGETKLETDRRLIAHRINALKKQLANLKKQRALRRKSRESGTIKTFA
                     LVGYTNVGKSSLFNRLTKSGIYAKDQLFATLDTTARRLYISPECSIILTDTVGFVSDL
                     PHKLISAFSATLEETAQADVLLHVVDAAAPNSGQQIEDVENVLREIHAGDIPCIKVYN
                     KTDLLPSEEQNTGIWRDAAGKIAAVRISVAENTGIDALREAIAESCAAAPNTDETEMP
                     "
     gene            complement(10977..12257)
                     /locus_tag="WGQ68_000936"
     CDS             complement(10977..12257)
                     /locus_tag="WGQ68_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEZ0714765.1"
                     /translation="MKSLFIRLLLLGSAAGVFYHTQNQSLPAGELVYPSAPQIRDGGD
                     ALHYLNRIRAQIGLHKLAHAPVLENSARRHARYLTLNPEDGHGEHHPDNPHYTAQKLT
                     ERTRLAGYLYNGVHENISTEEEAAESSDSDIRTQQRQVDGLMSAIYHRLSLLDRHTDE
                     AGAAFVRENGKTVLVFNQGNGRFERHCAQGRNQPEAGRKYYRNACHNGAVVYTDEAMP
                     AQELLYTAYPVGNGALPYFHGERPDPVPEYEITGNPASIDFSEAAGKITMKSFKLYQG
                     KNEIRPVRVLTAGNDPNGRLTAYQFALFPLKPLEYGTLYTAVFDYVRNGRRAQAKWQF
                     RTRKPDYPYFEVNGGETLAVRKGEKYFIHWRGRWCLEACTRYTYRQRPGSRLSIGRHE
                     AGGIVFSVDGMAGSRITLAPEGETERGVTLYLQD"
     gene            complement(12410..13135)
                     /locus_tag="WGQ68_000937"
     CDS             complement(12410..13135)
                     /locus_tag="WGQ68_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEZ0714766.1"
                     /translation="METQNKPTVTDIDRPILVPPGGHKKVLLHSCCAPCSGEVMEAML
                     ASGIDYTIYFYNPNIHPHKEYMLRKEENVRFAEKFGIPFIDKDDDYENDRKEWFAKAK
                     GMEFEPERGIRCTMCFDMRFEKAAQYAHEHGFPVFTSSLGISRWKNMAQINDCGHRAA
                     APYDDVVYWDFNWRKGGGSARMIEISKRENFYQQEYCGCAYSLRDSNAHRKSQGRIPI
                     KLGVLYYGDESTQYEPAPIRVDK"
     gene            complement(13208..13885)
                     /gene="radC"
                     /locus_tag="WGQ68_000938"
     CDS             complement(13208..13885)
                     /gene="radC"
                     /locus_tag="WGQ68_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEZ0714767.1"
                     /translation="MSIKQWPEGERPREKLLERGAAALSDAELLAILLRVGTRGMSAV
                     DLARYLLQEFGSLGRLMSAEVGKLSAYKGMGTASFTQFAVVREIGRRILAEELQESIV
                     LSDPDTVADYLRFHLGQEKVEVSVALLLNRQNQLIAVRELSRGTVAENTIYIREIVKL
                     ALDEYADSLIIAHNHPGGSPEPSQEDIMFTRRLAQAMSLVDVSLLDHFIVTAQTVRSF
                     RQLGLMP"
     gene            complement(14008..15357)
                     /gene="gshA"
                     /locus_tag="WGQ68_000939"
     CDS             complement(14008..15357)
                     /gene="gshA"
                     /locus_tag="WGQ68_000939"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEZ0714768.1"
                     /translation="MKLPVMSPEHSAQLQAFEAKILSNHAKIEAWFRTQWSVHRPPFY
                     GSVDIRNAGYKISSIDMNLFPGGFNNLNPNFIPLAAVAAQDAVQRACETAKSVLIIPE
                     NHTRNTFYLQNIYALGEILRSAGYEVRLGSLNPEVTEPTEFETALGDKILLEPLLRTR
                     DRVHLADGFSPCVVLLNNDLSAGIPDILKGISQTVLPPLHGGWTTRRKTNHFGAYNQV
                     TAEFAKLIGIDEWQINPYFEKIGGLDFQGREGEDALAEAVERVLAKIQAKYDESGITD
                     KPFVIVKADAGTYGMGVMSVKSADEVRGLNRKNRNKMAKVKEGLEVSEVIVQEGIYTY
                     ETLNGAVCEPVVYMMDRFVIGGFFRVHEGRGADENLNAGGMVFVPLSNSIPTGNGDNS
                     QEAPEACKRVFEQWDSLGMPRSEKDCDVDNEHNRLYVYGVMARLSLLAASIELEETA"
     gene            15617..17026
                     /gene="leuC"
                     /locus_tag="WGQ68_000940"
     CDS             15617..17026
                     /gene="leuC"
                     /locus_tag="WGQ68_000940"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HEZ0714769.1"
                     /translation="MTAQTLYDKLWNSHVVCEEEDGTVLLYIDRHLVHEVTSPQAFEG
                     LKMAGRKLWRIDSVVSTADHNTPTGDWDKGIQDPISKLQVDTLDKNIKEFGALAYFPF
                     MDKGQGIVHVMGPEQGATLPGMTVVCGDSHTSTHGAFGALAHGIGTSEVEHTMATQCI
                     TAKKSKSMLIAVDGKLKAGVTAKDVALYIIGQIGTAGGTGYAVEFGGEAIRSLSMEGR
                     MTLCNMAIEAGARSGMVAVDQTTIDYVKDKPFAPEGEAWDKAVEYWRTLVSDEGAVFD
                     KEYRFNAEDIEPQVTWGTSPEMVLDISSKVPNPAEETDPVKRSGMERALEYMGLEAGT
                     PLNEIPVDIVFIGSCTNSRIEDLREAAAIAKDRKKAANVQRVLIVPGSGLVKEQAEKE
                     GLDKIFIEAGFEWREPGCSMCLAMNADRLAPGQRCASTSNRNFEGRQGNGGRTHLVSP
                     AMAAAAAVTGRFTDIRMMA"
     gene            17123..17377
                     /locus_tag="WGQ68_000941"
     CDS             17123..17377
                     /locus_tag="WGQ68_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714770.1"
                     /translation="MNKLFITALSALALSACAGTWEGAKQDTARNLDKTQAAAERAAE
                     QTGNAVEKGWDKTKEAVKKGGNAVGRGISHLGGKIENATE"
     gene            17439..18080
                     /gene="leuD"
                     /locus_tag="WGQ68_000942"
     CDS             17439..18080
                     /gene="leuD"
                     /locus_tag="WGQ68_000942"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEZ0714771.1"
                     /translation="MKAFTKITAIVAPLDRSNVDTDAIIPKQFLKSIKRSGFGPNAFD
                     EWRYLDHGEPGMDNSKRPLNPDFSLNQPRYQGAQILLTRKNFGCGSSREHAPWALDDY
                     GFRAVIAPSFADIFFNNCYKNGLLPIVLTEEQVDRLFKEVEANEGYQLSIDLAEQTLT
                     TPSGETFTFDITEHRKHCLLNGLDEIGLTLQHADEIHAFEEKRRQSQPWLFNG"
     gene            18249..19520
                     /locus_tag="WGQ68_000943"
     CDS             18249..19520
                     /locus_tag="WGQ68_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEZ0714772.1"
                     /translation="MQQIKFIDLFSGMSGIRKGFEQACRKQSVACECVFTSEIKPAAL
                     EVLKQNYPDEVPYGDITKIETGDIPDFDILLAGFPCQAFSFAGKRLGFEDTRGTLFFD
                     VARILKAKKPKGFILENVEGLVTHDRKDPTQKIGRTLTVILETLETLGYYVSWKVLNA
                     KDFGIPQNRKRIYLTGSLKSKPDLSFETTLSPKLKNILESGLPTESSPFIKKLLKKFP
                     PSELYGKSVKDKRGGKNNIHSWDIELKGTVTEEEKQLLNMLLKERRKKKWASEIGIDW
                     MDGMPLTKAQISTFYKHPNLQNILDSLTDKGYLVLEHPKQKIGGRRIKDESLPKGYNI
                     VSGKKSFEINKILDPNDVAPTLVAMDMEHLFVVDNGGLRTLTGKEGLRLFGYPDDYSF
                     DIPKKDKYDLLGNTVAVPVIKAVSERLLHTL"
     gene            19536..20267
                     /locus_tag="WGQ68_000944"
     CDS             19536..20267
                     /locus_tag="WGQ68_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NgoBV family restriction endonuclease"
                     /protein_id="HEZ0714773.1"
                     /translation="MVKLTAQQIFDKLLNEEKILSVNGQIRFFLGDVDIIVKQKDVVG
                     NIIQEWLGGWLRKREIEFDVSTNTQMPPDFFLNKKDRSRELLEVKAFNRNAGPGFDIA
                     DFKMYSDEIIHKPYMLDVDYLIFGYDMDDNGNVTIKDLWLKKVWQITRSMDGWAINLQ
                     VKKGVVHKIRPGVWYSINKKNMPMFECLEDFVSAIEETVYQNPATRHNASLWKKKFEE
                     AYKKHYNRSISIPRWHEIAHKYKKK"
     gene            20301..21371
                     /gene="leuB"
                     /locus_tag="WGQ68_000945"
     CDS             20301..21371
                     /gene="leuB"
                     /locus_tag="WGQ68_000945"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HEZ0714774.1"
                     /translation="MTKHIAILRGDGIGPEIVAETVRVLDKLIAQGLDISYEYAPLGG
                     EAYDEYGHPYPEFTQNLCRKADAVLLGAVGSPQYDNLDRPLRPERGLLAIRKDLNLFA
                     NLRPAVLYPELANASTLKPEIVAGLDILIVRELTGDIYFGEPRGIRVLENGEREGYNT
                     MKYSESEIRRIAHVAFQSAQKRSKKVCSVGKANVLETTELWREIFEEIGKQYPDVELS
                     HMYVDNAAMQLVRAPKQFDVIATGNIFGDILSDEASMLTGSIGMLPSASLDENGKGLY
                     EPSHGSAPDIAGQNKANPLATILSLAMLLRYSLNDEARAQQVENAVQKVLQQGLRTSD
                     IYEEGTKLVSCSEMGDAILAAL"
     gene            21534..22097
                     /locus_tag="WGQ68_000946"
     CDS             21534..22097
                     /locus_tag="WGQ68_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HEZ0714775.1"
                     /translation="MKKIIFAALAAAAVGTASAATYKVDEYHANARFAIDHFNTSTNV
                     GGFYGLTGSVEFDQAKRDGKIDITIPVANLQSGSQHFTDHLKSADIFDAAQYPDIRFV
                     STKFNFNGKKLVSVDGNLTMHGKTAPVKLKAEKFNCYQSPMAKTEVCGGDFSATIDRT
                     KWGVDYLVNVGMTKSVRIDIQIEAAKQ"
BASE COUNT         5367 a         5845 c         5769 g         5251 t
ORIGIN      
        1 ggcatttcca aagggcattt taagccgtat ttgagagaat gcggatggcg ttttcagtgt
       61 tttcagcgtg ataaaattca aatttctatt ttaaaacaat cagcaaagca ggatttattt
      121 cagttgtccg agacaacggg cggctgcacc gcctgcactc cccatacttt ttcaatgcac
      181 tcgagcagga gtgagacgtg gggattatcc tcgtattctt ccgcataaat gaaattgagc
      241 ggcatactgt ggcggcacga ttcgataata gccacgcccg cgccttcttt tgccgccgct
      301 tcggctttgt ttcccggcac cattgccaca cctataccgc ctgcaaccaa atcgataatg
      361 gtttgggggt agtcgcacaa tatctgtttt ttgggtgaga gccggttgct gcgccaaaac
      421 tggtgcaggt gcttcctact tccggacacg cccgacattt ctatccatac gcattcttgc
      481 aggctctgcg gaagggagcg ggtcagatgg ggatattggc tttgcgggca aatcagcgcg
      541 taggtcaggt tttgcaggaa tacgctgcgg atgccccgtt gggcggcgtt gccgagtata
      601 aagccgccgt gcagggtttt gtgttggatg cgcgagagga tttcgccgct cattccgtat
      661 tggatgtgca ggcgcgtttt gggggctgtt tgaccgatat tgtccgtcag cgcgacgagc
      721 tttgccgaat cgatggggtg gataatgccc aaactggtct cttccgaata atcgcctgcc
      781 agcgttttgg caaaatactc caacttgtgt ttgtattgca gcagggattc cgcttcgggc
      841 aacagtattt cgcccgcccg cgtcaatacc atgcctttcc ccgtgcgcct gaacagcggc
      901 gtgccgacat attcttcaag ggctttaatt tgggcggaaa cggcaggctg ggaaaggaaa
      961 agtcgtttgg cggcttgggt aaggttgccc tcgtgcgcga cggcgacaaa tgattttaat
     1021 tgtacggcat ccatatatcc ctccttgtgc ggatgttttc tatatttgtg caatcgaaat
     1081 cttttaggtg gattgttgct gaaaattaac tttttaatca agtggtttgt aaattgtatc
     1141 agttttccgg atgatgatta ttaaaaaaaa agattggttt tattgcctgt ttgggcttta
     1201 aatggggtta cggcttccga acgcagcccg tatcaaaaag aaaagtcatg cgcccctttt
     1261 acgaggcgcg atatataagg aggaaggtta tggaaaaaca taatgggact tatcgggatt
     1321 tgcaccgtcc agcttcggaa tttgcgacgc gggacgaata tttggaacat gaattgcaga
     1381 ttatgcaacc aaaacgctgg cggcccaacc tgccctttcg cgattaccgc ttcgagtggg
     1441 aggatttgat tcctgcgatg gcgggaacga ttggaaaagt ggtgatggtg ggggcggtgg
     1501 cggcggcgtt tgccgcacct ttggggctgc ctgacagctt tgtactggaa aatgtgcgct
     1561 atgagctttt aatcgccgcc gcgtttatct tattggtatc gggctttttt ctgcccggcg
     1621 ccaacctgcc cggtacgcac gggccgctga ttccgatgat tcccatcgtt gtgtcggcag
     1681 gcgggcatcc tttggcgttc ggcatttcga ttgcggtttt aggtctgctg atggctttat
     1741 ttcgcggcgg cagtattatg gcgaagctga caagcaacgg cgtatgcggc ggattattac
     1801 tctatttggg ctttatcggc acgacggggc aggtaaaaaa attgttttcg tgggcaagcg
     1861 gttttaatat gccctacatc gcttttaccg tcattattgt aacgattgtg atgtacgctt
     1921 tgttggagca ttggaaaaaa cgctggttag ccgtgccttt gggatgcttg attgccggtg
     1981 tggtggcatt tgcattgggt gcgccgtttg agtttcacac cgcccccggc ctgcctccaa
     2041 tgagtcctgc ttattggtgg ggtgaaaaca gcggctggca tctggggttg ccgacggcag
     2101 aaagtttttt ggttgtcttt ccatttgcgg tattggctgt tgcaatgtgg tcgcccgatt
     2161 ttttaggaca tcaagtgttc caaaaaatca gctatccgga aaaaaccgat aaggtattga
     2221 tgaatataga cgacaccatg acaagttgtt ctgtccgtca agcagtgggt tctattttag
     2281 ggggtgcaaa ttttacctct tcttggggaa cttatatcgt accggcatcg attgccaaac
     2341 gccccattcc gggcggtgcg gttttaacgg cggttttatg tattatcgcc gggttatggg
     2401 gctatccgat ggacttggcg atttggcagc cggtattgag cgtagccttg gtcgtaggcg
     2461 tatacttacc gcttttggaa gcgggcatgg aaatgacgcg caaaggcaaa accacccaat
     2521 ccgccgccat cgtggtgttc tcttccgcct tggtcaatcc ggttttcggc tgggcgttga
     2581 cgatgctgtt ggataatttg ggcttaatcg gctgcaaaga gcgcagtgcg caattaggtt
     2641 tcgccggacg cgtgttgata cccgcagtag gtttcttgat cttgtgtgtg gcgatgggtg
     2701 cggtcgggat gctgcccggt atcccgccgt ttttggaaca cttcaaatct ttgggctagg
     2761 ctgaaatcgg aaatgccgtc tgaaccgctt tcagacggca tttttgcaaa caggcaaaat
     2821 gacggcggcg ggatttttta ttttcccgat tgaagtataa tgttgccggg cttcaactgg
     2881 atattcaaaa cggtttgttc caacactcgg aacggcgcat aaaacgccgc ccttcgcgtt
     2941 atcccgaacg gggcggctaa tcagattcta tcgccataaa aggcggggtt tcaaccgaaa
     3001 aggaattgag atgaataaaa ccttgtctat tttgccggtg gcaatcttac tcggtggctg
     3061 cgccgccgga ggcggtaaca cattcggcag cttagacggt ggcacaggca tgggcggcag
     3121 catcgtcaaa atggcggtcg ggagccaatg ccgtgcggaa ttggacaaac gcagcgaatg
     3181 gcgtttgacc gcgctggcga tgagtgctga aaaacaggcg gaatgggaaa acaagatttg
     3241 cgcttgcgtc gcccaagaag cacccgaaag gatgaccggc aacgatgtga tgcagatgct
     3301 ggctccgtcc acgcgcaatc aggcacttgc cgccctgacc gccaaaacgg tttccgcctg
     3361 cttcaaacac ctgtaccgct aaccttcttc agacggcatg aggcagatac cgtctgaagc
     3421 cgttttcctg caatttcgac agacgagaaa aatcatgaaa tacatcagca cgcgcggcga
     3481 aaccgcacac aaaccgttca gcgaagtttt attgatgggg cttgcacccg acggcggcct
     3541 gatgctgccg gaacattatc cgcaaatcgg gcgcgaaacc ttggacaaat ggcgcggttt
     3601 agcttatccc gaattggcgt ttgaaattat gcgcctgttt gttacggata ttccggagga
     3661 cgatttgcgc gacattctga accgtactta cacggaagcg gcgttcggta ctaaggaaat
     3721 cacccccgtc cgcacgcttt cagacggcat caaaatccaa gccttgtcca acggcccgac
     3781 gctggcgttc aaagatatgg caatgcagtt tttgggcaat gcgtttgaat atgttttaaa
     3841 caaagaaggc aaaaaactca atatcttggg cgcaaccagc ggcgatacgg gttcggctgc
     3901 ggaatatgcc ttgcgcggca aaaaaggtgt gaatgtattt atgctgtcgc ccgatggcaa
     3961 aatgagcgcg ttccaacgcg cgcaaatgta cagcctgcaa gacgggaata tccacaatat
     4021 cgccgtgaag ggaatgtttg acgactgtca ggatattgtg aaggcggtgc agaacgatgc
     4081 cgcgttcaag gaaaaatacc atatcggtac ggtcaattcg atcaactggg gacgcatcgt
     4141 cgcgcaagtg gtttattact ttgcaggcta tttcaacgcc acttcaagca atgacgaaac
     4201 cgtcagcttc tgcgtaccga gcggcaactt cggcaacgtt tgcgcgggac atattgccaa
     4261 acagatgggc ctgcctgtcc gccgcctgat tgttgcgacc aatgaaaacg atgtgttgga
     4321 cgagtttttc aaaaccggcg cataccgccc gcgcaacagc gcgcatactt atgttacctc
     4381 cagcccgtct atggacattt ccaaagcgtc caacttcgag cgtttcgtgt tcgacctgat
     4441 ggatcgcgat cctcaggaaa tcaatacgct gtgggcggaa gtagcggcgg gcaaaggctt
     4501 tgacctgcgg tttgctttag aaaaagtcgg tggcaaatac ggttttacct ccggcaaatc
     4561 cacccacccc gaccgcctcg ccaccatcag acaggtttat gagcaagacc aagaactcat
     4621 cgacccgcac actgccgacg gcgtaaaagt cgcccgcgaa gtgcgcgaag aaggggaaac
     4681 ggttgtgtgt ttggaaaccg cgttggcagc gaaattcgat gcgaccatac gcgaagccgt
     4741 cggcgatgcc gccattccgc gccctgccgc gctggaaggt ttggaaaacc tgccgcagcg
     4801 cgtgcaaacc gtgccgaaca gtgcggatgc ggtaaaaggc atcatcgaac aaacccttgc
     4861 ctgatattcc gtacaggggc attccgtccg aacttaagat tgaaagaaaa tgaccttacc
     4921 tatgcaggaa acccgttttt ctatactgct ggacgaactg gcggcaaaac aggaggcagc
     4981 catcgctccc cacctgcttg ccgacggaac caaggtatgg gtacgcaaag caggcaggca
     5041 caatgcgcgg tggcgttacg cgctgctcgg tatggttgcc cgatatttga aactgggggt
     5101 gttgaagccg gttcccagcc tcggcggcga gcctgccatt acaaccgaat caaaacgcct
     5161 gtacgaattg cgctcggcgg ggatagccgt tcccgaattg ctcgccgtca ggaaaaatgc
     5221 ccttatgttc ggcaatttgg caggcatccc gctcgatacg caaatccgcc aagaagccga
     5281 agccggaaag gcggacgctt ggcttgccgg tttggaagcc attgcacgcg tgcataaaaa
     5341 acagcagttc ctcagtcagg cgtttgcgcg gaatatgatg tcggacgggg aaaacatcag
     5401 ctttttggat tttgaagacg acccttccga agtcctgacc atagcgcaat gccaagcccg
     5461 cgattggctc tgctacatcc attcaaccgc gctgatattg aaaaacggcg gactgctgga
     5521 agcggctgcg gaaaaatggg gcggcgtatt gtcggatcag cctgccgaaa tacaaaagct
     5581 gattgccgcc accgtcaaac caatcctccc gatacgcagg ctggaacacc cacgctgggg
     5641 acgggacgcg ctcaggctgg cagcctcgat ttccctgatt tcccttgccg atataccgcc
     5701 ataagccgcc gcccgccccg cgatgccgtc tgaagccctt cagacggcat ataaccaacg
     5761 cgcatcaagc attgtcgcaa ccatccgttt cgtttagaat tttggcttta tcccgaatcg
     5821 ggcagacatt tccactctgc cgcacgtccc gaacaagcac tatccatttc ttttactgac
     5881 aggaaaccca aatggcagca ttcaataccc aaaaagtatt gtccgtacac cactggacag
     5941 acgcatattt tacctttacc tgcacccgcg acgaatcgtt gcgctttgaa aacggacagt
     6001 tcgttatggt cggattgatg gtggacggca agccgctgat gcgtgcatac agcgtcgcct
     6061 ccgccaactg ggaagaacac ctcgaatttt tcagcattaa agtccaagac ggcccgctga
     6121 ccagccgcct gcaacacctc aaagtcggcg acgacgtgtt aatcagcaaa aaaccgaccg
     6181 gaactctggt tgccggcgac ctgaatccgg gcaaacacct ttatttgttg agcaccggta
     6241 ccggcatcgc ccctttcttg agcatcacca aagaccccga aatttacgag caatttgaaa
     6301 aaatcatcct cgtacacggc gtgcgctaca aaaaagattt ggcgtactac gaccgcttta
     6361 ccaaagaatt gcccgagcac gaatacctcg gcgacttggt taaagaaaaa ctgatttact
     6421 acccgattgt ttcccgagaa gaattcgaac accacggccg cctaaccgac ctgatggtga
     6481 gcggcaaact gtttgaagac atcggcctgc ccaaaatcaa tcctcaagac gaccgcgcga
     6541 tgctgtgcgg cagccccgcg atgctgaaag acacctgcaa agttttggac gatttcggac
     6601 taaccgtttc tccgaaaaca ggcgtgcgcg gcgattacct gattgagcgc gcgtttgtgg
     6661 atcaataagc tgcttgaaaa tgccgtctga acggcatcag cgataaaaaa acccgaatcc
     6721 tgccaatcag gattcggggt ttttatagtg ggtaaaaatt agaaatacac atttccgtca
     6781 tttccgcgca ggcgggaatc cagacctatc cgtttcatta gcatttgaaa ataaaagaaa
     6841 aaccaagcat atggattccc ccctacgcga gactgacgac ggtgttctgc agtagccaaa
     6901 gcagaaaacc atccttattg tcatttcatc atgatataac aataaggatg ttaaaaatta
     6961 gttatcatta tatgcatgat atataagaaa aattgtccca ataagaaatg ttatgcagaa
     7021 aataaaatga aaaggatggt tttcattgaa aatggttgtt ctgccaccgt catacacaga
     7081 accggtattt ggaaaaaaaa taatccatat tgacatcatt attccattta tcgcataaat
     7141 ccacttttta atttttttcc tcattaatat aatctcttat ttaataaaat caaataccta
     7201 gcaattcgat ctcaaatttt aaacgccaac gtgtttgagc ggtaacaatg catactttaa
     7261 gtttgtaatc cttcatcgtc tcatttttgt tttaatcgac tatacgtcta aagcaacgcc
     7321 gtccggcatt gttcggacgg cattttttca gacggcattt tcccccgttt gtgaaggcgc
     7381 aaagcgttgc ccaaaaccgc cagcgaactg aaactcatac ccagtgcggc tatccacggt
     7441 tggacatagc ccaaaacggc aagcggtacg gcaatgatat tgtacgcgcc cgcccatatc
     7501 aggttttggc ggataatctg ctgcgtgcgg cgggcttggg caaacatatg cggcacgatg
     7561 ttcaaatcct cgttgagcag aacgatgtcc gcaccgtccc gggcaatgtc cgttccgccg
     7621 gctgcggcgg cggatacgtc tgcctgagcc aaaacgggcg cgtcgttgat gccgtcgcct
     7681 atcatcagca cttttttccc ttctttttgc aaggctttga cgtattccag tttgtcctcg
     7741 ggcatggctt gggcgcggta gtgcgcgaca cccagggcgc gtgcggtttc ggcaacggcg
     7801 gtttcgcggt cgccgctgag gatgtgcagg gtcaggtttt tgcctgccaa ctgccgcacc
     7861 gcctccgccg cgctgtcttt caaggggtcg gtcaggtaga acacggcttg gaaaccgctt
     7921 tgactgccga ggtaaaccgc gctgccgccg ccttctgttt gcggttcttt acctgaaatt
     7981 tcggcgacat aggatgccct gcccaatgcc caaacctgtg tttcgccgtt gacggtcagt
     8041 tgcgcgccca cgccttcgcc gatgcggttg aggcgttgtt taatagcaat gtcggggacg
     8101 ctgccgtctg aaatgcggca gttgaggatg gcgcgggcaa ggggatgttc ggactgttgt
     8161 tctaaagcct gcgccaccgc gagaacaaag gcttcgtctg tgcctctcaa caatgagata
     8221 cggcggacgg cgggtttgcc ttgggtcagc gtgccggttt tgtcgaagat gatgtcggtg
     8281 gtttgggcga gggtttcgat tgcctgcttt ccgccgatta aaataccttc gcgcgccagc
     8341 gtaccggtag aagctgccag cgcggtcggc gtggcaagcg ataaggcgca ggggcaggta
     8401 atgaccagca gggcgacggt aatccacaat gcggtgtgcg cgtcggcgta cagcgtccag
     8461 ccgatgaaga cggggacggc aagcaggagt tcgccgaata tgaaagacga ggcgtattgt
     8521 tccgccaact cggcagtgcg cggtttttgc gctaaggcgc ggtcgagcag gcggacgatg
     8581 tgcgacagtc gcgtgccgcc gccggtgcgg tcggtgcgta taatcagggg gctttgcgtg
     8641 ttgagtgtgc cggcggttac tttttcagac ggcattttgg cgacgggcag gctctcgccg
     8701 gtcagcatag attcgttgac ggcactactt ccttccagca ccgtgccgtc aacggggatg
     8761 gtttcgcccg gtttgaccag cacgatatcg cccgccttca atttgacgac agctgcctcg
     8821 caggtttcct gcgtatcggg gtaatcgggc atatgatggc aaaacgcagg aatcagcttc
     8881 accagcctct cggcggcatc gcctgcctta cggcgggcaa tgtgttccat aaagcgtccg
     8941 cccagcagga aaaacagcag catcgcgatg gattcgaaat acatcccctg ccccgcattt
     9001 gtcgcaaggc tgtaaacgcc ggcgataaag gtcatgatga tggcgacggt aatcggcgta
     9061 tccatgccga cgcggcggtt tttcaagtcg cgcagcgcgc cttgataaaa cgggacggcg
     9121 caatagaata cgacgggcag caccattaaa aagccgcccc aatggaggat ttgcaggaaa
     9181 tcgggttcga tgtcgccgcc gtaaaggtag gtcggcagcg cgaacatcat cgtctgcatc
     9241 atccccagcc cggcaacggc gaggcggacg atgtattgtt tgcgttcttt ttggttggcg
     9301 gcttcgattt tttgcgcgtc atagggtgcg gcggtgtagc ctatctgcct gattttcaac
     9361 agaatgtcgg aaaggcggat tttgccgtcg tcccagacga cgcggcagcg gtgcgtgctg
     9421 taattgagat cgatgcggac gatgccgtct gtacgcaaaa gctgctgttc gatcagccag
     9481 acgcaggcgg cgcaggtgat gccgccgagc attaaaaccg cctcgcgcgt gccgccgtgg
     9541 gtttccacaa agtcggactg gacttcgggc aggtcgtaca ggcggatttg gtcgaggatt
     9601 tcttggggcg gcagctcggt tttttgcgcg tcggcggtgc gttgtttgta ataactgccc
     9661 aagcccgcgt caataatgct ttgtgcgacc gcctgacagc cggcgcagca ggtttctcgg
     9721 tcttcgcctt cgtagcggac ggtcagatgc aggttttcgg gaacatccag cccgcagtgg
     9781 aaacaggttt ttttcatggc atttcggttt cgtctgtgtt tggtgcggcg gcacaagact
     9841 cggcaatggc ttcgcgcagt gcgtctatac cggtattttc agcaacggaa atgcggacgg
     9901 cggcaatttt tcccgcagcg tcgcgccata tgcccgtgtt ttgttcttca gacggcagca
     9961 ggtcggtttt gttgtacacc ttgatgcacg gaatatcgcc ggcatggatt tctcgcagta
    10021 cgttttccac gtcttcaatc tgctgtccgc tgttcggagc ggcggcatcg acgacgtgca
    10081 gcagcacatc ggcttgcgcg gtttcttcca gcgtggcgga aaaggcggaa atcagtttgt
    10141 gcggcagatc gctgacgaat ccgacggtat cggtcaggat gatgctgcat tcgggactga
    10201 tgtacagccg ccgcgccgtg gtgtcgagtg tggcgaaaag ctggtctttc gcatatatgc
    10261 ccgacttggt cagccggttg aacaggctgg atttgccgac attggtatag ccgaccagcg
    10321 caaacgtttt gattgtgccc gattcgcggg acttgcggcg cagggcgcgc tgttttttga
    10381 ggttggcaag ctgttttttc aaggcattga tccgatgggc gatcaatcgg cggtcggttt
    10441 ccagtttggt ttcgccgggg cctttcatgc cgataccgcc gcgctggctc tgcagatggc
    10501 cgtaaccgcg tatcaagcgt cccgccaaat ggctcaattg cgccaactcg acttgcagcc
    10561 tgccttcctg cgtgcgggcg cggcgagcga aaatcgccag aatcagccct accctgtcca
    10621 atacgcggca tttcagttct ttttcaaggt tgcgttcctg cgtgggcgtg agttcgtggt
    10681 tgaatacgac caaatcgatg ccgtctgcgg caactgcttc tgacagctcc gccgccttgc
    10741 ccgtgccgac aaacagcgcg gtgtgcggac ggtcgcgttt ggcagtctcc acgcgcacgg
    10801 aatcgccgcc cgccgctttg accagctcga cagcttccgc caatgccgtc tgaaaaccgt
    10861 tcagacgggc ggcactactg cccgtaccat ctttgtccaa cattacgccc accagcatga
    10921 cgcgttccgg ttgcgcttgg gtactgccgt tgcggcctgt tctgcctgtc atgtattcaa
    10981 tcctgtaaat aaagggttac gcctcgttcc gtttctcctt ccggtgcaag cgtgatgcgg
    11041 ctgcccgcca ttccgtcaac gctgaagacg atgccgcccg cctcgtgcct tcctatggac
    11101 aggcggctgc cgggtcgctg ccggtaggta taacgggtac acgcttccaa acaccagcgt
    11161 ccgcgccagt ggatgaaata tttttcgcct tttctaaccg caagtgtctc gccgccgttt
    11221 acctcaaaat aagggtaatc gggttttcgg gttctaaact gccatttcgc ctgcgcgcgc
    11281 cgtccgttgc ggacatagtc gaataccgcc gtataaagcg taccgtattc caaaggcttg
    11341 agcgggaaaa gcgcgaattg gtacgcggtc agcctgccgt tggggtcgtt gccggcggtt
    11401 aaaaccctga cggggcggat ttcgttttta ccctgataca gcttgaaact tttcatcgta
    11461 attttgcctg ccgcctcgga aaaatcaatg ctggcaggat tgcccgtgat ttcatattcc
    11521 ggcacggggt ctggacgctc gccgtggaaa taaggcagtg cgccgttgcc gacgggatag
    11581 gctgtataga gcagctcctg tgcgggcatg gcttcgtcgg tgtacacgac cgcaccgtta
    11641 tggcaggcgt tgcggtaata tttccgtcct gcttccggct gatttctgcc ttgggcgcaa
    11701 tgccgctcaa acctgccgtt gccctgattg aatacgagaa cggttttacc gttttcgcgc
    11761 acaaatgccg ctcctgcctc atccgtatgg cggtcaagta gggaaaggcg gtggtagatt
    11821 gcgctcatta atccgtccac ttggcgttgc tgcgtgcgga tgtcgctgtc ggacgattcg
    11881 gcggcttcct cttccgtgct gatgttttca tgcacgccgt tgtagagata cccggcaagg
    11941 cgtgtgcgtt cggtcagctt ttgtgcggtg tagtgcggat tgtcgggatg gtgttcgccg
    12001 tgtccgtctt cgggattgag cgtgagatag cgtgcgtgcc tgcgggcgga attttccaaa
    12061 accggcgcgt gtgccagctt gtgcaaaccg atttgggcgc ggatgcggtt gaggtagtgc
    12121 agcgcatcgc cgccgtccct gatttgcggt gcggacggat agacaagttc gcccgcgggc
    12181 agggattggt tttgggtatg atagaaaacg ccggccgccg aacccaacag gagcagccga
    12241 ataaaaaggg atttcataat gccgtcctcc cgacgcggca acggggtgtt ttatcaaaac
    12301 ggcaaacttg ccaatgcggt ttccgattgc agattgccaa accggcaaac ctttccccgt
    12361 gccgatgccg aacctgatgc cgtctgaacc ttcagacggc atcggatgtt tatttgtcca
    12421 cccggatggg tgcaggttcg tattgtgtcg attcgtcccc gtaatacagc acgccgagtt
    12481 tgatggggat tctgccctgc gatttgcggt gggcattgga atcgcgcaag gaataggcgc
    12541 aaccgcaata ttcctgctgg tagaagtttt cacgtttgct gatttcaatc atgcgcgcgc
    12601 tgccgccgcc tttgcgccag ttgaaatccc aatacaccac atcatcgtaa ggcgcggcgg
    12661 cgcggtgtcc gcagtcgttg atttgcgcca tatttttcca gcgtgaaatg cccagcgaac
    12721 tggtaaagac ggggaacccg tgttcatgcg cgtattgcgc cgccttttca aaacgcatat
    12781 cgaaacacat ggtgcagcgg atgccgcgtt ccggctcaaa ctccatgcct ttggctttgg
    12841 caaaccattc tttgcggtcg ttttcgtagt cgtcgtcttt atcgatgaaa ggaatgccga
    12901 acttttccgc aaagcgcacg ttttcctctt ttcggagcat atactctttg tgcggatgga
    12961 tattgggatt gtaaaaataa atagtgtaat caatgccgct ggccagcatg gcttccatca
    13021 cttcgccgct gcacggggcg caacaggaat gcagcaagac ttttttatgt ccgccgggcg
    13081 ggacgagtat ggggcggtca atgtcggtaa cggtaggttt gttttgtgtt tccattaact
    13141 taacgatatc tgtcgaatac ggttgaagcg gctattttat cagagccgcc gcatgtaaaa
    13201 cagggtgtca gggcatcagc ccgagctgcc tgaacgaacg gacggtttgc gccgttacga
    13261 taaaatggtc gagcagcgac acatcgacca gcgacattgc ctgtgccagc cgccttgtga
    13321 acatgatgtc ttcctgcgaa ggttcgggcg agccgcccgg atggttgtgc gcaataatca
    13381 ggctgtcggc atattcgtcc aatgccagtt tgacgatttc gcggatgtaa atcgtgtttt
    13441 ccgcaaccgt accgcgcgac agctctctga ccgcaatcag ttggttttgg cggttcagca
    13501 gcagcgcgac gctgacttcg actttttcct gccccaaatg aaagcgtaaa taatcggcca
    13561 ccgtatccgg atcggacagg acgatgctct cctgcaattc ttccgccaat atccgccgcc
    13621 cgatttccct gactacggca aactgtgtga aacttgccgt ccccatccct ttgtatgccg
    13681 acagtttgcc gacctccgcg ctcatcagcc tccccaaact gccgaactcc tgcagcaaat
    13741 aacgcgccaa atcgaccgca ctcattccgc gcgtgccgac gcgcagcagg attgccaaaa
    13801 gttcggcatc actcaaagcc gccgccccgc gttccaacag cttttccctg ggcctttccc
    13861 cctccggcca ttgcttgatg ctcatgtttt ccctttttta aatagtctga gatgccgtct
    13921 ggaacaaatg ttcccacacg ccaaatatcc gaaatgccgt ctgaaccgga tttccgcttc
    13981 agacggcatc tgtatttcaa aacagtctta cgccgtttct tccaactcga tggacgccgc
    14041 cagaagcgac aggcgtgcca ttacgccgta aacgtagagg cggttgtgtt cgttgtccac
    14101 gtcgcagtct ttttcagagc gcggcatacc cagcgagtcc cattgttcga atacgcgctt
    14161 gcaggcttcg ggcgcttctt gggaattatc gccgttaccg gtaggaatgc tgttagacag
    14221 cggaacaaac accataccgc cggcgttgag gttttcgtcc gcaccgcgcc cttcgtgtac
    14281 gcggaaaaag ccgccgatga cgaagcggtc catcatatac acgacgggtt cgcacaccgc
    14341 gccgtttaag gtttcataag tataaatccc ttcttggaca atcacttcgc tgacttccaa
    14401 gccttctttg actttcgcca ttttattgcg gtttttacgg ttcaatccgc gcacttcgtc
    14461 ggcggatttg acgctcatca cgcccatgcc gtaagtgccg gcatcggctt tgacgatgac
    14521 gaaaggtttg tcggtaatgc ccgattcgtc gtatttggct tgaattttcg ccagcacacg
    14581 ttctaccgct tccgccaacg cgtcttcgcc ttcacgccct tggaagtcca aaccgccgat
    14641 tttttcaaaa taagggttaa tttgccattc gtcgatgccg attaacttgg caaattcggc
    14701 ggtaacttgg ttgtacgcgc cgaaatgatt tgttttgcgg cgcgtcgtcc aaccgccgtg
    14761 caacggcggc aaaacggttt ggctgatgcc tttgaggatg tcgggaatgc cggcagacaa
    14821 atcgttgttc aacaaaacca cgcaaggcga aaagccgtct gcaagatgga cgcggtcgcg
    14881 ggtacgcaat aaaggttcca acaggatttt gtcgcccaat gcagtttcaa attcggtcgg
    14941 ttcggttact tccggattca ggctgcccaa gcgcacttca taccctgccg aacgcaaaat
    15001 ctcgccgagg gcgtaaatgt tttgcaggta aaacgtattg cgcgtgtggt tttcgggaat
    15061 aatcaatacg gatttcgccg tttcgcaggc gcgttgcacc gcatcttgcg cggcaaccgc
    15121 cgccagcggg ataaagttgg gattcagatt attgaagccg ccggggaaca aattcatatc
    15181 gatagacgaa attttgtaac cggcattgcg tatatcgacc gaaccgtaaa acggcgggcg
    15241 gtgtacgctc cactgcgtgc ggaaccacgc ctcgattttg gcgtgattgg acaggatttt
    15301 tgcctcaaac gcctgaagtt gcgccgaatg ttcgggcgac atcaccggta atttcatctt
    15361 tcgtctgcct ctgtgtgttt tttgacaaga tgcagatgat aatgcctttg aaagaatgac
    15421 gacaagtatt ctttcgtatc gaatcatgct ttagatataa ctgtttactg aaatggacaa
    15481 tacgtctaat tttttcgtta aaattgtttg caaaataaca ccggctcaaa aattagacaa
    15541 aatttgttgc gcggtataaa gaatacgtct aaaatccgcc caatccgcat taccatttat
    15601 ccaaaagaac aacatcatga cagcacaaac cctctacgac aaactttgga acagccacgt
    15661 cgtctgcgaa gaagaagacg gcaccgtcct gctctacatc gaccgccatt tggtgcacga
    15721 agttaccagc cctcaggcat ttgaaggctt gaaaatggcg gggcgcaagc tgtggcgcat
    15781 cgacagcgtc gtctccaccg ccgaccacaa caccccgacc ggcgattggg acaaaggcat
    15841 ccaagacccg atttccaagc tgcaagtcga tactttggac aaaaacatta aagagtttgg
    15901 cgcactcgcc tattttccgt ttatggacaa aggtcagggc atcgtacacg ttatgggccc
    15961 cgaacaaggc gcgaccctgc ccggtatgac cgtcgtctgc ggcgactcgc acacttccac
    16021 ccacggcgca ttcggcgcac tggcgcacgg catcggcact tccgaagtcg agcacacgat
    16081 ggcgacccag tgcattaccg cgaaaaaatc caaatccatg ctgattgccg ttgacggcaa
    16141 attaaaagcg ggcgttaccg ccaaagacgt ggcactttac atcatcgggc aaatcggcac
    16201 ggcgggcggt acaggctacg ccgtcgagtt tggcggcgaa gccatccgca gcctttctat
    16261 ggaaggccgc atgactttat gcaatatggc gatcgaagcc ggtgcgcgct caggcatggt
    16321 tgccgtcgac caaaccacca tcgactatgt aaaagataaa cccttcgcac ccgaaggcga
    16381 agcgtgggac aaagccgtcg agtactggcg cacgctggtg tctgacgaag gtgcggtatt
    16441 cgacaaagaa taccgtttca acgccgaaga catcgaaccg caagtcactt ggggtacctc
    16501 gcctgaaatg gttttagaca tcagcagcaa agtgccgaat cctgccgaag aaaccgatcc
    16561 ggtcaaacgc agcggtatgg aacgcgccct tgaatacatg ggcttggaag ccggtacgcc
    16621 attaaacgaa atccccgtcg acatcgtatt catcggctct tgcaccaaca gccgcatcga
    16681 agacttgcgc gaagccgccg ccatcgccaa agaccgcaaa aaagccgcca acgtacagcg
    16741 cgtgttaatc gtccccggct ccggtttggt taaagaacaa gccgaaaaag aaggcttgga
    16801 caaaattttc attgaagccg gttttgaatg gcgcgaaccg ggctgttcaa tgtgtctcgc
    16861 catgaacgcc gaccgcctcg caccggggca acgctgcgcc tccacctcca accgcaattt
    16921 tgaaggccgt caaggcaacg gcggacgtac ccacctcgtc agccctgcca tggcagccgc
    16981 agccgcagtg accggtcgct ttaccgacat ccgcatgatg gcgtaagtat tttcaggcgg
    17041 tctttaagaa tttgacgcgg ccgtctgaaa tatgtcccgt aaggcagaaa cacctttaat
    17101 attcaaacca aggagtttaa ccatgaacaa acttttcatt accgccctgt ccgccctcgc
    17161 cttgtccgcc tgcgccggca cttgggaggg cgcgaaacaa gacaccgccc gcaaccttga
    17221 caaaacacag gccgccgccg aacgcgccgc cgaacaaaca ggcaacgccg tcgaaaaagg
    17281 ctgggacaaa accaaagaag ccgtcaaaaa aggcggcaat gccgtcggac gcggcatttc
    17341 ccatctcggc ggaaaaatcg aaaacgccac cgaataaccg attcgcgatg ccgtctgaaa
    17401 ccgcttcaga cggcatcgtc agcacaagac gcaaacccat gaaagccttt accaaaatta
    17461 ccgccatcgt cgccccgctc gaccgcagca acgtcgatac cgacgccatc atccccaaac
    17521 aatttttaaa atccatcaaa cgcagcggct tcggccccaa tgcctttgac gaatggcgtt
    17581 acctcgacca cggcgaaccg ggcatggaca acagcaaacg cccgttgaac cctgatttct
    17641 cgctgaacca gccgcgttac caaggcgcgc aaatcctgtt gacgcgtaaa aacttcggtt
    17701 gcggctcttc acgcgaacac gccccttggg cattggacga ctacggcttc cgcgccgtca
    17761 tcgcccccag cttcgccgac atcttcttta acaactgcta caaaaacggc cttttaccta
    17821 tcgtgttgac cgaagaacaa gtcgatcggc ttttcaaaga agttgaagcc aacgaaggct
    17881 atcagctctc catcgacctt gccgaacaaa ccctgaccac accaagcggc gaaacattca
    17941 cattcgacat taccgaacac cgcaaacact gcctcttaaa cggcttggat gaaatcggtc
    18001 tgaccctgca acacgccgac gaaattcacg ccttcgaaga aaaacgccgc caaagccagc
    18061 cttggctgtt taacggttaa gcctatgccg tctgaagacc gttcagacgg cattcctgat
    18121 aacatataat tgctgcaact aatgtaaaca gaggggctat aaattgcttt catctttcaa
    18181 gcgggcttag caacgctcta aattaatgca atacagctct atttcaaata aatttttaga
    18241 tttttaatat gcaacagatt aaatttattg acttattttc cgggatgagc ggtatcagga
    18301 aaggatttga acaagcctgt cggaaacagt cggttgcttg cgaatgtgtt tttacctcag
    18361 aaatcaaacc ggcagctttg gaagtattga agcaaaacta ccctgatgaa gtgccgtatg
    18421 gagatataac gaaaattgaa acgggagata ttcccgattt tgacatcttg ttggcaggct
    18481 tcccttgcca ggctttttct ttcgccggaa aaagattggg ctttgaagat acgcggggaa
    18541 cgcttttctt tgatgtggca aggattttaa aggcaaaaaa accaaaaggt tttattttgg
    18601 aaaatgtgga aggattggtg acgcacgata gaaaagatcc gacacaaaaa ataggacgca
    18661 ccctgaccgt tattttggaa accttggaaa cattgggcta ctatgtttct tggaaagttt
    18721 taaatgcaaa agatttcggc attccccaaa acaggaagcg catttatctg acaggcagtt
    18781 tgaaatccaa acccgacttg tcttttgaaa caaccctaag tccgaaatta aaaaatattt
    18841 tggaatcggg actgcctacc gaaagcagtc ctttcatcaa aaaattgcta aagaaatttc
    18901 ccccgtccga actgtacgga aaatcagtaa aggacaaacg gggagggaaa aacaatatcc
    18961 acagttggga tattgaatta aaaggcacag taaccgaaga agagaagcaa ttattaaata
    19021 tgcttctaaa agaacggagg aagaaaaaat gggcttcaga aatcggcatc gattggatgg
    19081 atgggatgcc tttgacaaaa gcgcaaattt caactttcta taaacacccc aatcttcaaa
    19141 atattttgga cagcctgaca gataaaggct atttggtttt ggagcatccg aaacaaaaaa
    19201 ttggcgggcg aagaatcaaa gatgaatccc tgcccaaagg ctacaatatt gtttcaggaa
    19261 aaaaatcatt tgaaatcaat aaaatattag acccaaatga tgttgcgcca accttagttg
    19321 caatggatat ggagcacctt ttcgtcgttg acaacggcgg tttgcgcaca cttaccggaa
    19381 aagaaggttt acgcttattc ggctatccgg acgattattc gtttgatatt cctaaaaaag
    19441 acaaatacga tttattgggc aataccgttg ccgttcctgt gattaaggcg gtatctgaaa
    19501 ggcttctgca tactttataa accgataggg ataaaatggt aaaacttact gcacaacaaa
    19561 tatttgataa gttgctgaat gaggaaaaaa tcttatcggt caatggtcaa attagatttt
    19621 tcttgggcga tgtggatatt atcgtcaaac aaaaagatgt cgtcggcaac atcattcaag
    19681 aatggctcgg cggatggttg agaaaaaggg aaattgaatt tgatgtttca accaataccc
    19741 aaatgccccc cgatttcttt ttaaataaaa aagaccgcag tagagaattg ttggaagtaa
    19801 aggcgttcaa ccgaaatgcc ggcccgggtt ttgatattgc agattttaaa atgtattctg
    19861 atgaaatcat tcataaaccc tatatgctgg atgtagatta tttaatattc ggttacgata
    19921 tggacgacaa cggcaatgta accatcaagg atttatggct taaaaaagta tggcaaatta
    19981 cgagaagcat ggatgggtgg gcaattaatc ttcaagtcaa aaaaggcgtg gtgcataaaa
    20041 tccgcccggg tgtttggtac agcataaata aaaagaatat gcctatgttt gaatgcttgg
    20101 aagattttgt ttccgcaatt gaagaaaccg tttatcaaaa cccggcaaca cggcataacg
    20161 catccttatg gaaaaagaaa tttgaggaag cctataaaaa acactacaac cgatcgattt
    20221 ccatacctcg ttggcacgaa attgcacaca aatacaaaaa gaaataaccc ttcaaccatc
    20281 aacccataag gaaacttgaa atgaccaaac atatcgccat cctccgaggt gacggcatcg
    20341 gtcccgaaat cgtcgccgaa accgtccgcg tactcgacaa actcatcgcc caaggcttgg
    20401 acatcagcta cgaatacgcc ccattgggtg gtgaagccta cgacgaatac ggccatcctt
    20461 atcccgaatt tacgcaaaac ctctgccgca aagccgatgc cgtcctgctt ggtgcagtcg
    20521 gttctcctca atacgacaac ctcgaccgtc cgttgcgccc tgagcgcggc ctgttggcaa
    20581 tccgtaagga tttgaacctg tttgccaatt tgcgtcctgc tgttttatat cccgaattag
    20641 ccaacgcctc cacgctgaag cctgaaatcg ttgccggttt ggatattttg attgtgcgcg
    20701 aactcaccgg cgacatctat ttcggcgaac cgcgcggcat ccgcgttctg gaaaacggcg
    20761 aacgcgaagg ctacaacacc atgaaataca gcgaaagcga aatccgccgt attgcccatg
    20821 ttgccttcca atccgcccaa aaacgcagca aaaaagtctg ttccgtaggc aaagccaacg
    20881 ttttggaaac caccgaactg tggcgcgaaa tctttgaaga aatcggcaaa caatatcccg
    20941 atgtcgaact ctcccatatg tatgtggaca acgccgctat gcagctcgtg cgcgcgccca
    21001 aacaatttga cgtgattgcc accggcaaca tcttcggcga tattttatcg gacgaggcct
    21061 ctatgctgac cggctccatc ggtatgctgc cttctgcttc tttggacgaa aacggcaaag
    21121 gcctgtacga accgtctcac ggctccgctc ccgacatcgc cggacaaaac aaagccaacc
    21181 cgctggccac catcctctcg ctcgcaatgt tgctgcgtta cagcctgaac gacgaagcgc
    21241 gcgcgcaaca agttgaaaac gccgtccaaa aagtgctgca acaaggcttg cgtaccagcg
    21301 acatttacga agaaggcaca aaactcgttt cctgctccga aatgggcgat gcgatactcg
    21361 ccgccttgta aaaaaacaaa gcgcggatca caccgcgctt tttcatctgc ccttttccgc
    21421 ccgcaccgtt cgtcaaccgc ctgcaaaccc accgtttacc ggcaggaaat gccataaatc
    21481 cccgcctatg ttaggataaa cacctttatc ggcccacaca aaggattatc atcatgaaaa
    21541 aaatcatctt cgccgcactc gcagcggcag ccgttggcac tgcctccgcc gccacctaca
    21601 aagtggacga atatcacgcc aacgcccgtt tcgccatcga ccatttcaac accagcacca
    21661 acgtcggcgg tttttacggt ctgaccggtt ccgtcgagtt cgaccaagca aaacgcgacg
    21721 gtaaaatcga catcaccatc cccgttgcca acctgcaaag cggttcgcaa cactttaccg
    21781 accacctgaa atcagccgac atcttcgatg ccgcccaata tccggacatc cgctttgttt
    21841 ccaccaaatt caacttcaac ggcaaaaaac tggtttccgt tgacggcaac ctgaccatgc
    21901 acggcaaaac cgcccccgtc aaactcaaag ccgaaaaatt caactgctac caaagcccga
    21961 tggcgaaaac cgaagtttgc ggcggcgact tcagcgccac catcgaccgc accaaatggg
    22021 gcgtggacta cctcgttaac gttggtatga ccaaaagcgt ccgcatcgac atccaaatcg
    22081 aggcagccaa acaataaatc ttgatgcgaa tcagcacaaa tgccgtcttc atcaaaccgc
    22141 cccgccaacg gtaagccgat atggcggagc acaatccgcc gaaagatatt tcagcggcag
    22201 gcagggcttg ccggaaagtc cggaaagtat ag
//
LOCUS       DAWFHY010000029        21967 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.029, whole genome shotgun sequence.
ACCESSION   DAWFHY010000029 DAWFHY010000000
VERSION     DAWFHY010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21967
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.029"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            1..249
                     /locus_tag="WGQ68_000947"
                     /pseudo
     CDS             1..249
                     /locus_tag="WGQ68_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060537127.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            303..854
                     /locus_tag="WGQ68_000948"
     CDS             303..854
                     /locus_tag="WGQ68_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714776.1"
                     /translation="MKVLNGWSDRKMWRVLSALPIGVVFFDLIYGFVLNVLQGLDLQR
                     AVPDSEGVLAVTPDIAFNSLQIVANGGMAAVVCFGLAVVFLLNRSVRRRQVLEIGVFR
                     MLGLVAVLAFSAPSVWEWANALPLLLKGADVVNTGNARYVLTALCMPFPAVSCVIGLV
                     GRFRLQTASGRAAKSGGAGKADG"
     gene            complement(1117..1808)
                     /locus_tag="WGQ68_000949"
                     /pseudo
     CDS             complement(1117..1808)
                     /locus_tag="WGQ68_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002255623.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
     gene            complement(2114..2671)
                     /locus_tag="WGQ68_000950"
     CDS             complement(2114..2671)
                     /locus_tag="WGQ68_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714777.1"
                     /translation="MMQKHKCLIDFILTVASQIAYADLPLSLEELLTDKGKFKLESSI
                     SYINTERNQSEFANPIYVQTSATKIATVMAMMITPVMAQNLDSQVFDSQNVKAIQLSQ
                     AEMKETQGEFVPIIAAAAFGGALGAWEYHGANLYNHGKLGTAQGAATAAGIGAATGVA
                     AKQGWLLLPEADWLGIWHGDRVFMH"
     gene            complement(2916..3074)
                     /locus_tag="WGQ68_000951"
     CDS             complement(2916..3074)
                     /locus_tag="WGQ68_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714778.1"
                     /translation="MKPPLPICTLSALAACTLSGQGGSRVYGEIKARDLCVTAPILLS
                     AFCLACSR"
     gene            complement(3071..3913)
                     /locus_tag="WGQ68_000952"
     CDS             complement(3071..3913)
                     /locus_tag="WGQ68_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0714779.1"
                     /translation="MLVCNPYEVVIHGTTSSGKIFRPSDWAERLCGILSSFTKDHRLS
                     YSKWVRPILVDNIRCVAVDKKLETDNPQMFRFLMDFAADNDLRVIDCKALLEEREQGG
                     QNNPADEHVMLAQAIEEKHAAEKAQEQTASGASYVLREIGADDTATAFAALSVLRSAL
                     TDINRFTEQINKVQRPQGYRLLGIFEEGKHNAVAVCGFREACTLASGRHIHIDDIVTL
                     PQSRRKGYASRLLEEVRKIGAETGVTKIHLNVHVNHDRADAHRLYFKNGFEICAYHFR
                     CDPK"
     gene            4152..5072
                     /gene="pyrB"
                     /locus_tag="WGQ68_000953"
     CDS             4152..5072
                     /gene="pyrB"
                     /locus_tag="WGQ68_000953"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HEZ0714780.1"
                     /translation="MPNPLYRQHIISISDLSREQLECLLQTALKLKAHPRGDLLEGKL
                     IGSCFFEPSTRTRLSFETAVQRLGGKVIGFSDGANTSAKKGETLADTARIISGYTDAI
                     IQRHPKDGAARVAAEFSRVPVINAGDGTNQHPSQTLLDLVTIYETQGRLDKLKIAMAG
                     DLKYGRTVHSLCQALKRWNCEFAFVSPPSLAMPDYITEELDEAGCRYRILGSLEEAAE
                     WADILYMTRVQRERFDEQEFAKIQGKFNLEASMLARAKPNLRVLHPLPRVDEIHPDVD
                     ATPHAYYFEQATNGVYARMAILSLVLNEEV"
     gene            5082..5540
                     /locus_tag="WGQ68_000954"
     CDS             5082..5540
                     /locus_tag="WGQ68_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HEZ0714781.1"
                     /translation="METPKLSVEAIEKGTVIDHIPAGRGLTILRQFKLLHYGNAVTVG
                     FNLPSKTQGSKDIIKIKGVCLDDKAADRLALFAPEAVVNTIDNFKVVQKRHLNLPDEI
                     AEVFRCPNTNCAGHGEPVKSRFYVKKHNGQTRLKCHYCEKTYSRDSVAEA"
     gene            5605..6270
                     /locus_tag="WGQ68_000955"
     CDS             5605..6270
                     /locus_tag="WGQ68_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714782.1"
                     /translation="MMMHASVQSRFAPILYVLIFFAGFLTAQIWFNQKAYTEELPPLL
                     SALSAVALVWLAWAFVSARSKAKAEKFYREKMIQNESIHPVLHASLQHLEHKPQILAL
                     LVKNHGKGMAEQVRFKAEVLPDDEDARTIAAELAKMDMFALGTDAVASGETYGRVFAD
                     IFELSAALEGRAFKGMLKLTAEYKNIFGDACRSETALELGALNQALQEISKTSEKSKR
                     IFY"
     gene            complement(6333..7550)
                     /locus_tag="WGQ68_000956"
     CDS             complement(6333..7550)
                     /locus_tag="WGQ68_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEZ0714783.1"
                     /translation="MQRRIITLLCAAGMAFSTQTLAANLEVRPNAPERYTVKQGDTLW
                     GISGKYLYSPWQWGRLWDANRDQIHNPDLIYPDQVLVLRHVDGEPRLGLEQTDGIPVV
                     KMSPDKEVSGYGIPAIDVNFYRIFMRHPQIVSRKETAAAPRLLSGPEGRLLYTKGTRV
                     YTKGLKEPGRYLTYRINKNITDPDTGKFLGQEVAFSGIVRSLDYTDSVLEQRSKQAGE
                     RPKDNEYHTRTHPLITPLRTPSIQPLVVETAISEIQQGDYLMKMPEDTDRFNMMPHEP
                     SRPVQAKIVSVFEGTRIAGQFQTITIDKGEADGLDKGTVLSLYKRKKTMQVDLSNNFK
                     SRDTVELISTPAEEVGLAMVYRTSEHLSSAIILENISDISVGDTAANPGRDLDNIPDQ
                     GRSRVKFGFNRSE"
     gene            7692..8237
                     /gene="def"
                     /locus_tag="WGQ68_000957"
     CDS             7692..8237
                     /gene="def"
                     /locus_tag="WGQ68_000957"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEZ0714784.1"
                     /translation="MKNRRRIRRRKEKIMALLNILQYPDERLHTVAKPVEQVDERIRK
                     LIADMFETMYESRGIGLAATQVDVHERVVVMDLTEDRSEPRVFINPVIVEKDGETTYE
                     EGCLSVPGIYDTVTRAERVKVEALNEKGEKFTLEADGLLAICVQHELDHLMGIVFVER
                     LSQLKQGRIKTKLKKRQKHTI"
     gene            8324..9250
                     /locus_tag="WGQ68_000958"
     CDS             8324..9250
                     /locus_tag="WGQ68_000958"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEZ0714785.1"
                     /translation="MKVIFAGTPDFAAAALKAVAAAGFEIPLVLTQPDRPKGRGMQLT
                     APPVKQAALELGLRVAQPEKLRNNAEALQMLKEVEADVMVVAAYGLILPQEVLDTPKH
                     GCLNIHASLLPRWRGAAPIQRAIEAGDAETGVCIMQMDIGLDTGDVVSEHRYAIQPTD
                     TANEVHDALMEIGAAAVVADLQQLQSKGRLNAVKQPEEGVTYAQKLSKEEARIDWSES
                     AAVIERKIRAFNPVPAAWVEYQGKPMKIRRAEVVAQQGAAGEVLSCSADGLVVACGEN
                     ALKITELQPAGGRRMNIAAFAAGRHIEAGTKL"
     gene            9329..10588
                     /gene="rsmB"
                     /locus_tag="WGQ68_000959"
     CDS             9329..10588
                     /gene="rsmB"
                     /locus_tag="WGQ68_000959"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEZ0714786.1"
                     /translation="MSMALAQKLAADSIAAVAEGRNLQDVLAQIRTAHPDLTAQENGA
                     LQDIAYGCQRYLGSLKHMLAQMLKKPIDNPQLESLLLAALYQLHYTRNAPHAVVNEAV
                     ESIAKIGRGQYRSFANAVLRRFLRERDKLAASCKKDDVAKHNLPLWWVAYLKNHYPKH
                     WHNIAAALQSHPPMTLRVNRRHGNAESYLEKLAAEGIAAKALDEYAVTLEEAVPVNRL
                     PGFSDGIVSVQDFGAQQAAYLLNPKDGERILDACAAPGGKTGHILELADCRVTALDID
                     AGRLKRVEDNIARLGFQTASAACADARDLAAWYDGKPFDAILADVPCTASGVARRNPD
                     VKWLRRPTDALKTARQQEALLDALWQTLKQGGRMLLATCSVFVEENDGQLQKFLNRHA
                     DAEPIESRVLLPNKHQDGFYYALIQKH"
     gene            10554..11153
                     /locus_tag="WGQ68_000960"
     CDS             10554..11153
                     /locus_tag="WGQ68_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEZ0714787.1"
                     /translation="MAFITRLFKSIKQWLVLLPMLSVLPDAAAEGIDVSRAEARITDG
                     GQLSISSRFQTELPDQLQQALRRGVPLNFTLSWQLSAPIIASYRFKLGQLIGDDDNID
                     YKLSFHPLTNRYRVTVGAFSTDYDTLDAALRATGAVANWKVLNKGALSGAEAGETKAE
                     IRLTLSTSKLPKPFQINALTSQNWHLDSGWKPLNIIGNK"
     gene            11153..13273
                     /locus_tag="WGQ68_000961"
     CDS             11153..13273
                     /locus_tag="WGQ68_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HEZ0714788.1"
                     /translation="MRRFLPIAAICAVVLLYGLTAATGSTSSLADYFWWIVAFSAMLL
                     LVLSAVLARYVILLLKDRRDGVFGSQIAKRLSGMFTLVAVLPGVFLFGVSAQFINGTI
                     NSWFGNDTHEALERSLNLSKSALNLAADNALGNAIPVQIDLIGAASLPGDMGRVLEHY
                     AGSGFAQLALYNAASGKIEKSINPHKLDQPFPGKARWEKIQRAGSVRDLESIGGVLYA
                     QGWLSAGTHNGRDYALFFRQPVPKGVAEDAVLIEKARAKYAELSYSKKGLQTFFLATL
                     LIASLLSIFLALVMALYFARRFVEPVLSLAEGAKAVAQGDFSQTRPVLRNDEFGRLTK
                     LFNHMTEQLSIAKEADERNRRREEAARHYLECVLEGLTTGVVVFDEQGCLKTFNKAAE
                     QILGLPLTPLWGSSRHGWHGVSAQQSLLAEVFAAIGAAAGTDKPVHVKYAAPDDAKIL
                     LGKATVLPEDNGNGVVMVIDDITVLIHAQKEAAWGEVAKRLAHEIRNPLTPIQLSAER
                     LAWKLGGKLDEQDAQILTRSTDTIVKQVAALKEMVEAFRNYARSPSLKLENQDLNALI
                     GDVLALYEAGPCRFAAELAGEPLMVAADTTAMRQVLHNIFKNAAEAAEEADVPEVRVK
                     SEAGQDGRIVLTVCDNGKGFGREMLHNAFEPYVTDKPAGTGLGLPVVKKIIEEHGGRI
                     SLSNQDAGGACVRIILPKTVKTYA"
     gene            13266..14543
                     /locus_tag="WGQ68_000962"
     CDS             13266..14543
                     /locus_tag="WGQ68_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent Fis family transcriptional
                     regulator"
                     /protein_id="HEZ0714789.1"
                     /translation="MRSSDILIVDDEIGIRDLLSEILQDEGYSVALAENAEEARKLRH
                     QARPAMVLLDIWMPDCDGITLLKEWAKNGQLNMPVVMMSGHASIDTAVEATKIGALDF
                     LEKPISLQKLLSTVENALKYGAAQTETGPVFDKLGNSAAIQEMNCEVEAAAKRTSPVF
                     LTGEAGSPFETVARYFHKNGTPWVSPARVEYLIDMPMELLQKAEGGVLYVGDISQYSR
                     NIQAGIAFIVGKAEHRRVRVVASGSRAAGSDGIACEEKLAELLSESVVRIPPLRMQHE
                     DIPFLIQGIACNVAESQKIAPAAFSEEALAALTRYDWPGNFDQLQSVVATLLLEADGQ
                     EIGAAAVSSLLGQNVPAEGAEDMVGGFNFNLPLRELREEVERRYFEYHIAQEGQNMSK
                     VAQKVGLERTHLYRKLKQLGIGVSRRAGEKTEE"
     gene            14634..15821
                     /gene="dprA"
                     /locus_tag="WGQ68_000963"
     CDS             14634..15821
                     /gene="dprA"
                     /locus_tag="WGQ68_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEZ0714790.1"
                     /translation="MTEDERFAWLQLAFTPYIGAESFLLLLRRFGSASNALSAPAEQV
                     APAVRHKQAAEAWRNAEKRASARQAAEAALEWEMRDGCRLMLLQDEDFPEMLTQGLTA
                     PPVLFLRGNVRLLHKPSAAIVGSRHATPQAMRIAKDFGRTLSGKGIPVVSGMASGIDT
                     AAHQGALQAEGGTIAVWGTGIDRIYPPSNKNLAYEIAEKGLIVSEFPIGTRPYAGNFP
                     RRNRLIAALSQVTLVVEAALESGSLITAGLAAEMGREVMAVPGSIDSPHSKGCHKLIK
                     DGAKLVECLDDILNECPGLLQNTGASSYSINKDTPDTGRRAVQTAYAPPPAAKMPSEA
                     AAGGTAHGGILDKMGFDPVHPDVLAEQLAMPAADLYAALLELELDGSVAAMPGGRYQR
                     IRT"
     gene            15847..16308
                     /locus_tag="WGQ68_000964"
     CDS             15847..16308
                     /locus_tag="WGQ68_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein"
                     /protein_id="HEZ0714791.1"
                     /translation="MTEVIAYLIEHFQDFDTCPPPEDLGMLLEEAGFDTMEIGNTLMM
                     MEVLLNSSEFSAEPADSGALRVYSKEETDNLPQEVMGLMQYLIEEKAVSCEQREIIIH
                     ALMHIPGDEITVDTAKVLTLLLLWANKSELPVLVGDELMSALLLDNKPTMN"
     gene            16380..18686
                     /gene="topA"
                     /locus_tag="WGQ68_000965"
     CDS             16380..18686
                     /gene="topA"
                     /locus_tag="WGQ68_000965"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEZ0714792.1"
                     /translation="MAKNLLIVESPSKAKTLKKYLGGDFEILASYGHVRDLVPKSGAV
                     DPDNGFAMKYQLISRNGKHVDAIVAGAKEAENIYLATDPDREGEAISWHLLEILKSKR
                     GLKNIKPQRVVFHEITKNAVLDAVAHPREIEMDLVDAQQARRALDYLVGFNLSPLLWK
                     KIRRGLSAGRVQSPALRLICERENEIRAFEAQEYWTVHLDSHKGRSKFTAKLAQYNGA
                     KLEQFDLPNEAAQADVLKELEGKEAVVTAIEKKKRSRNPAAPFTTSTMQQDAVRKLGF
                     TTDRTMRTAQQLYEGIDVGQGAIGLITYMRTDSVNLADEALTEIRHYIENKIGKEYLP
                     SAAKQYKTKSKNAQEAHEAIRPTSVYRTPESVKPFLSADQFKLYQMIWQRTVACQMTP
                     AKFDQTTVDITVGKGVFRVTGQVQTFAGFLSVYEESSDDEEGEDSKKLPEMSEGDKLP
                     VDKLYGEQHFTTPPPRYNEATLVKALEEYGIGRPSTYASIISTLKDREYVTLEQKRFM
                     PTDTGDIVNKFLTEHFAQYVDYHFTAKLEDQLDEIAGGKRQWIPLMDKFWKPFIKQVE
                     EKEGIERAKFTTQELDETCPKCGEHKLQIKFGKMGRFVACAGYPECSYTRNVNETAEE
                     AAERIAKAEAEQAELDGRECPKCGGRLVYKYSRTGSKFIGCANYPKCKHVEPLEKPKD
                     TGVQCPQCKKGNLVERKSRYGKLFYSCSTYPDCNYATWNPPVAEECPNCHWPVLTIKT
                     TKRWGVEKVCPQKECGWKEQIEPPAPKE"
     gene            complement(18759..19454)
                     /locus_tag="WGQ68_000966"
     CDS             complement(18759..19454)
                     /locus_tag="WGQ68_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714793.1"
                     /translation="MMKDLNLSNSLFKGYNDKHGLMICGYEWGWSKADEAAYVAGEYK
                     LPENKIDHTFANKSLYFGEQAKKWRYDNTIKNWFEMWGHPLDENGLGGAFEKSLVQTN
                     WAATQGNTIDNPDKFTQPEHIDNFLYHIEKLRPKVILFMGSRLADFLNNQNVLPRFEQ
                     LVGKQTKPLETVQKEFDGTRFNVKFQSFEDCEVVCFPHPSASRGLSYDYIALFVPEMN
                     RILSDFKTTRGFK"
     gene            complement(19474..20025)
                     /locus_tag="WGQ68_000967"
     CDS             complement(19474..20025)
                     /locus_tag="WGQ68_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714794.1"
                     /translation="MEFLSLHPWWSFFIILTIILSYIGFCAHFHIQNGAVFFYSYRDV
                     TIVFLTPVILIALSYLIKNKEILSACILCITLIAFSWAWIIIYRPNTKRFFLSLLIVW
                     GKLLLSTIVWAILAISLGLAVITGNSKRKKYERRDRHAERNEKAFIGALLVGFELSRN
                     LLNLCCLHKDFSTPSKNIEVNRS"
     gene            20144..20521
                     /locus_tag="WGQ68_000968"
                     /pseudo
     CDS             20144..20521
                     /locus_tag="WGQ68_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679508.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            20610..21179
                     /gene="rsmD"
                     /locus_tag="WGQ68_000969"
     CDS             20610..21179
                     /gene="rsmD"
                     /locus_tag="WGQ68_000969"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEZ0714795.1"
                     /translation="MAAGKHTKHSNRVRIIGGQCRGRKLSFTSADGLRPTPDSVREKL
                     FNWLGQDLTGKTVLDLFGGSGALGIEAASRNAKRVLISDNNRQTVQTLQKNSRELGLG
                     QVQIVFSDGIAYLKTVSEQFDVVFLDPPFAWQDWQILFDALKPCLNPRAFVYLEAGTL
                     PNIPDWLTEYREGKSGQSTFELRVFQVAE"
     gene            21302..21553
                     /locus_tag="WGQ68_000970"
     CDS             21302..21553
                     /locus_tag="WGQ68_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhL family 4Fe-4S dicluster ferredoxin"
                     /protein_id="HEZ0714796.1"
                     /translation="MSLFITDECINCDVCEPECPNDAISQGEEIYEINPNLCTQCVGH
                     YDEPQCQQVCPVDCILIDQEHPETHDELMAKYEKIIQFK"
     gene            21650..21733
                     /locus_tag="WGQ68_000971"
     tRNA            21650..21733
                     /locus_tag="WGQ68_000971"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21684..21686,aa:Tyr,seq:gta)
     gene            21764..21837
                     /locus_tag="WGQ68_000972"
     tRNA            21764..21837
                     /locus_tag="WGQ68_000972"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21796..21798,aa:Gly,seq:tcc)
     gene            21847..21921
                     /locus_tag="WGQ68_000973"
     tRNA            21847..21921
                     /locus_tag="WGQ68_000973"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21879..21881,aa:Thr,seq:ggt)
BASE COUNT         5442 a         5537 c         6037 g         4951 t
ORIGIN      
        1 agccatttta gcttcgctga aacttcgttt tcgtatcaat cacagcacac attttgctga
       61 acgacaaaac catattaatg gaattgagaa cttttggaac caggcaaaac gtcatttacg
      121 caagtttaac ggcattccca aagcgcattt tgagctgtat ttaaaggagt gcgaatggcg
      181 ttttaacaac agtgagataa aagttcttgt tccattttaa aacaattagt aaaatcaagt
      241 ttgtcctagt tatctaggac agccccttgt tttttgttcg gcggcttgcg tggtcgggta
      301 aaatgaaagt tttgaacggt tggtcggaca ggaagatgtg gcgggttttg agtgctttgc
      361 cgataggcgt ggtgtttttt gatttgatct acggttttgt gttgaatgtg ttgcagggtt
      421 tggatttgca gcgtgccgtg ccggattcgg aaggcgtgtt ggcggttacg cccgatattg
      481 cattcaacag tttgcagatt gtcgccaacg gcggtatggc ggcggtggtc tgtttcgggt
      541 tggcggttgt gtttttgctc aaccgttcgg tgcggcggcg gcaggtgttg gaaatcgggg
      601 tgttccggat gttggggctg gtggcggtat tggcgttcag tgcgccgtcg gtgtgggagt
      661 gggcgaacgc gctgccgctg ctgctgaagg gcgcggacgt ggtcaatacg gggaatgcgc
      721 gttatgtgct gacggctttg tgtatgccct ttccggcggt gtcgtgcgtc atcgggctgg
      781 tggggcggtt caggcttcag acggcatcgg gcagggcggc aaagtcaggg ggtgcgggca
      841 aggcggacgg ataggacgca tttttcagcg ggtgcgtcga gaagcagccg atgtgtttgg
      901 cagccgcagc ttggggggta gtgctaatgg cggtttcttt gcttttatag tggattaaca
      961 aaaaccagta cggcgttgcc tcgccttagc ccaaagagaa cgattctcta aggtgctgaa
     1021 gcaccaagtg aatcggttcc gtactatccg tactgtttgc ggcttcgtcg ccttgtcctg
     1081 attactatat aaaataaatg ggcaaaaatc ggtttattat cgtttttgcc gcatttggat
     1141 ttgttctacc gtaaaacgtg tttgacgaac gggattctta ttaaaaaaca tctgatttct
     1201 aacaaaatca gtattttttg gcacgatggc taaaattttt ccttccattt cgccatcacg
     1261 tgttttccat gcgctcaaga attgtgattt gctcattgag acgtgcccca gcgatggatc
     1321 agccagcaaa acagtttctc cgttaatacc gttcaatacc gaaaaatggt tgtttttacg
     1381 gtattttaaa tacacaatta caggaatttt tagttgtacc aactgttcaa atggcaaagc
     1441 ataaccttgt gcttcaaaac ccagttcggg cattatgcgt tgcatatcat caaaagaagc
     1501 acgcatttgg gttttatcca ttttgtctaa gatttccgct tcagaataat gtctgccata
     1561 aaaattattc agtaacgtgg caatcgaagc cgcgccgcaa gaaaaatcca aatcttgttt
     1621 tactatgccg gaatctcgcc gtgctttcca actccgtaca tggatgtttt ggtaagaagc
     1681 ggggggtcaa caaacatagg ccaagcaaaa actatatttg gggcgaaacc aatcaaagcc
     1741 gcataattta tcaatttata aagatttttt atcataatat gtatacgcgg aataaacgca
     1801 tgaaacataa aaaaataaaa tcatatgcaa ttttattgca tgaataaata tgaataaaat
     1861 agataatgat gggagtaaat acgccatgta ttttggaagt ttaaatttat taataataaa
     1921 ataatttatc gtagcgcaaa taaatcccaa aattggaacg attagaaaaa aaattaatac
     1981 acccatcatc atgcaaactc tatattaata aatagctaac taatgctatt gtggaaatta
     2041 gttagctaac taaaagttat taatgattat tttcgagaat tgactgcatt attagcagca
     2101 ttggcaccaa aacctagtgc atgaataccc ggtctccatg ccaaattccc agccaatccg
     2161 cctccggcag cagcagccag ccctgttttg ccgctactcc tgttgccgca ccgattcctg
     2221 ccgcagtagc cgcgccttgc gcagttccta atttaccatg attatacaaa ttagcaccat
     2281 gatactccca tgcacctaat gcaccgccaa aagcagcggc tgcaataatg ggaacaaatt
     2341 caccttgtgt ttctttcatt tcagcctgtg ataattgaat tgctttcaca ttttggctgt
     2401 caaaaacttg gctgtctaaa ttttgcgcca ttacaggtgt aatcatcata gccattacag
     2461 ttgcaatttt cgttgcgctg gtttgcacat aaataggatt agcaaattcg ctttgattgc
     2521 gttcagtgtt gatgtagcta atactgcttt ctagtttgaa tttacccttg tcagtcaata
     2581 attcttccaa acttaaaggt aagtcagcat aagcaatttg gctggcaaca gtcaaaataa
     2641 aatctattag acatttgtgt ttttgcatca tttcgtttga ttttctagtt tttgagaatg
     2701 atacaaagtt tttttacaaa gtaaagagtc actctgaaaa acattttttc attataaatc
     2761 aaaatattga tagaataaat agcgagcatc gattcacggt gtactttagt gcaaagcgta
     2821 caacgcgagc ctgaaccacc agccgcaaca ggaaaagaaa gccgatagag tgcaatgctt
     2881 gccaacgtgc aagcgagctt gcaagaacgc ttggctcaac gagagcaggc aagacagaaa
     2941 gcagaaagca ggataggagc ggtaacgcaa aggtctcggg ctttgatttc gccgtaaacc
     3001 ctgctgccgc cttgtccgga aagggtgcag gcggcgagtg ccgacagggt gcagatgggg
     3061 agggggggtt tcatttgggg tcgcaacgga agtggtatgc gcagatttca aaaccgtttt
     3121 tgaaatacag gcggtgcgcg tcggcacggt cgtggttgac gtggacgttg aggtggattt
     3181 tggttacccc tgtttccgcg ccgattttgc ggacttcttc caaaaggcgc gaggcgtagc
     3241 ctttgcggcg gctttgcggc agggtaacga tgtcatcgat gtggatgtgg cggccgctgg
     3301 cgagggtgca ggcttcgcgg aagccgcaga cggcgacggc attgtgtttg ccttcttcaa
     3361 aaatacccag caggcggtag ccttgggggc gttggacttt gttgatctgt tcggtaaagc
     3421 ggttgatgtc ggtcagggcg gaacgcaaaa cgctcaaggc tgcaaaggcg gtggcggtgt
     3481 cgtccgcgcc gatttcgcgc aaaacgtagg atgcgcccga ggcggtctgt tcctgtgctt
     3541 tctcggcggc gtgtttttct tcgattgcct gtgccagcat gacgtgttcg tcggcagggt
     3601 tgttttgtcc gccctgttcg cgttcttcga gcagggcttt gcagtcgatg acgcgcaggt
     3661 cgttgtcggc ggcaaagtcc atcaggaagc ggaacatttg gggattgtcg gtttccagtt
     3721 ttttatcgac ggcgacgcag cggatgttgt ccaccagtat ggggcgcacc catttcgaat
     3781 aggaaagcct gtggtctttg gtgaacgagg acagaatgcc gcacaggcgt tccgcccagt
     3841 cgctgggacg gaaaatcttg ccggaactcg ttgtgccgtg gatgacgact tcgtaggggt
     3901 tgcagactaa catggcggct tcctgaaaag aaatgtctag cgcgattata ccttatgctt
     3961 atgcgggcgt gtttggatat gccgtctgaa aagtacggga ttcgtgcggt aaaactttgc
     4021 ggcggcaaat gtgcgataat acgcgccgta ttgccgcttt tgcgaagctg ttccgcaaac
     4081 atacgggcgg cgtggacgac gtataaccgg atacccgcct gacgcgggtt ttttacggaa
     4141 ggggggcaaa aatgcctaat ccgctttaca gacagcatat catctccatt tcggatttgt
     4201 cgcgcgaaca gttggaatgc ctgcttcaga cggcattgaa gctgaaggcg catccgcgcg
     4261 gcgacctgtt ggaaggcaaa cttatcggtt cgtgcttttt cgagccgtcc acgcgcacga
     4321 ggctgtcgtt tgaaacggcg gtgcagcgtt tgggcggcaa ggtcatcggt ttctcggacg
     4381 gcgcgaatac cagtgccaaa aaaggcgaga cgcttgccga taccgcccgc atcatttccg
     4441 gatatactga tgctatcatc caacgccacc ccaaagacgg cgcggcgcgc gtggcagcgg
     4501 agttttcgcg cgtccccgtt atcaacgccg gcgacggcac gaaccagcac cccagtcaga
     4561 cgctgctcga cctggttacc atttatgaaa cacagggacg tttggacaag ctcaaaatcg
     4621 ccatggcggg cgacttgaaa tacggacgta ccgtgcattc gctttgtcag gcgttgaaac
     4681 gctggaattg tgaatttgcc tttgtttcgc cgcccagcct agccatgccc gactatatta
     4741 ccgaagagtt ggacgaagcc ggctgccgat accgtatcct cggtagtttg gaagaagcgg
     4801 cggaatgggc ggatatcctg tatatgaccc gcgtccagcg cgaacgtttc gacgaacagg
     4861 aatttgccaa aatccaaggc aaattcaacc tcgaagcgtc tatgctcgcc cgcgccaaac
     4921 cgaacctgcg cgtgctgcac cccctgccgc gcgtggacga aatccatccc gatgtcgatg
     4981 ccacgccgca cgcctattat ttcgagcagg cgaccaacgg cgtttatgcg cgtatggcga
     5041 tattgtcgct ggtgttgaac gaagaagtgt gaggaaccga tatggaaacc ccgaaactca
     5101 gtgtcgaagc cattgaaaaa ggtacggtta tcgaccatat tcccgccggc agggggctga
     5161 ccatcctgcg ccagttcaaa cttttgcact acggcaacgc ggtaaccgtg ggcttcaacc
     5221 tgcccagcaa aacccaaggc agcaaagaca tcatcaaaat caaaggcgtg tgcttggacg
     5281 acaaagccgc cgaccgcctc gccctgttcg cccccgaagc ggtggtcaac accatcgaca
     5341 atttcaaggt cgtgcagaag cggcatttga acctgcccga cgaaatcgcc gaagtgttcc
     5401 gctgtccgaa cacgaattgc gccggccacg gcgagccggt caaaagccgg ttttatgtta
     5461 aaaagcacaa cgggcagacg cggctgaaat gccactactg cgaaaaaacc tacagccggg
     5521 attcggtggc ggaagcctga cggattccct taaaccgagt gggcggcatt tcgtctgccg
     5581 cctgttttgc caatctgaaa tggaatgatg atgcacgctt ctgtccaaag ccgtttcgca
     5641 ccgatacttt atgttttgat tttctttgcc ggttttttga ccgcgcaaat ctggttcaat
     5701 cagaaagcct atactgaaga gctgcctccg cttctgtccg cattgtccgc cgtcgcgctg
     5761 gtgtggctgg cgtgggcgtt cgtgtcggcg cgttcaaagg ccaaggcgga aaagttctac
     5821 cgcgaaaaaa tgatacagaa cgaaagcata caccccgtcc tgcacgcctc tttgcaacac
     5881 ttggaacaca agccgcaaat actcgccctg ctggtcaaaa accacggcaa agggatggcg
     5941 gaacaggtca ggttcaaggc ggaagtgctg cccgacgacg aagacgcgcg cacgattgcc
     6001 gccgagttgg caaaaatgga tatgttcgca ttggggacgg acgcggtcgc ctcgggcgaa
     6061 acctatggac gcgtgttcgc cgatattttc gagttgtcgg cggctttgga agggcgcgcg
     6121 ttcaaaggaa tgttgaaact gacggcggaa tataaaaaca tcttcggcga tgcctgccgt
     6181 tcggaaacgg cgttggagtt gggcgcactc aatcaggcgt tgcaggagat ttcaaaaaca
     6241 tcggaaaagt ccaaacggat attttattga agatggaaaa atgccgtctg aaacggaagg
     6301 tgtttcagac ggcatttttg tcggatgatt aattattcgg agcggttgaa gccaaacttc
     6361 acgcggctgc ggccctgatc cggtatattg tccaaatcgc gtcccggatt ggcggcggtg
     6421 tcgcctacgg aaatatcgga gatgttttcc aaaatgatgg cggacgacag gtgttcggag
     6481 gtgcggtaaa ccattgccaa gcccacttct tcggcaggag tggaaatcag ctcgacggta
     6541 tccctgcttt tgaaattgtt ggagaggtcg acctgcatcg ttttcttgcg tttgtagagg
     6601 ctcaaaaccg tgcctttgtc caaaccgtcc gcctcgcctt tgtcgatggt gatggtttga
     6661 aactggccgg caatccttgt gccttcaaac acggaaacga ttttagcctg aaccgggcgg
     6721 gacggttcgt gcggcatcat gttgaagcgg tcggtgtctt ccggcatttt catcaggtag
     6781 tcgccctgct gtatttcgga aatggcggtt tcgaccacca gcggctgtat cgaaggggtg
     6841 cgcagcgggg taatcaaagg atgggtgcgg gtatggtatt cgttgtcttt cggccgttct
     6901 ccagcctgtt tcgagcgttg ttcgaggaca gagtcggtat agtcgaggga gcgcacgatg
     6961 ccgctgaatg cgacttcctg cccgaggaat ttacccgtat ccggatcggt gatgttttta
     7021 ttgattcggt aggtcaggta gcggcccggc tctttcaggc ctttggtgta aaccctggtg
     7081 cctttggtgt acagcagcct gccttccggg cccgagagca ggcgcggcgc ggcagcggtt
     7141 tctttgcggg aaacgatttg cgggtgccgc ataaagatgc ggtagaagtt gacatcgatg
     7201 gcgggaatac cgtatccgga cacttcctta tccggactca ttttgacgac ggggatgccg
     7261 tctgtctgtt ccaagccgag gcgcggttcg ccgtcaacgt ggcgcaacac caatacctgg
     7321 tccggataaa tcaggtcggg attgtggatt tgatcccggt tcgcgtccca caggcggccc
     7381 cattgccacg ggctgtacag gtatttgccc gaaatgcccc acagggtgtc gccctgtttg
     7441 accgtgtagc gttccggcgc gttcgggcgc acctccaaat ttgccgccaa agtttgtgtt
     7501 gagaatgcca tacctgccgc gcagagcagg gttataatac gacgttgcat aaccgttccc
     7561 cttatctgat aaatttcggt ttgtcttgct tgattgggtt ggaaaaagcg gcggcagccc
     7621 ctcgggatgt gccgcgtgat aaaaaatgtt ccgcatttta acatcgaatt atccgcacca
     7681 tcacggtaat tatgaaaaac aggcggcgta tccgccgaag gaaagagaaa attatggctt
     7741 tattgaatat cttgcaatat cccgacgagc gtctgcacac ggtggcaaag cctgtcgaac
     7801 aagtcgacga gcgcatccgg aagctgattg ccgatatgtt tgaaacgatg tacgaatcgc
     7861 gcggcatcgg gctggcggcg acgcaggtcg atgtgcacga gcgcgtggtc gtgatggatt
     7921 tgaccgaaga ccgcagcgaa ccgcgcgtgt tcatcaaccc cgtcatcgtt gaaaaagacg
     7981 gcgaaaccac ttacgaagag ggctgcctgt ccgtgccggg catttacgac accgtaaccc
     8041 gcgccgaacg cgtcaaggtc gaggctttga acgaaaaagg cgaaaagttc acgctggagg
     8101 cggacggctt gctggcgatt tgcgtgcagc acgagttgga ccacctgatg ggcatcgtgt
     8161 ttgtcgaacg cctgtcgcag ctcaagcagg gacgcatcaa aaccaagctg aaaaaacgtc
     8221 agaaacatac gatttgaccc ttttgccgtg ccgtctgaac gctgcaaagt tttcagacgg
     8281 cacggtcttg tccgacaacc ttacgcatac gcaggaatat gctatgaaag tcatctttgc
     8341 cggcacgccc gattttgccg ccgccgcctt aaaagccgtt gccgccgccg gttttgaaat
     8401 cccgctggtg ctgacccagc ccgaccgtcc gaaagggcgc ggtatgcaac tgaccgcccc
     8461 gcccgtcaaa caagccgcgc tggaactcgg tttgcgcgtg gcacagcccg aaaaactgcg
     8521 taacaacgcc gaagccctgc aaatgctcaa agaggtcgag gcagacgtaa tggtggttgc
     8581 cgcctacggt ttgattctgc cgcaggaagt gttggatacg ccgaaacacg gctgcctcaa
     8641 catccacgct tcgctgttac cccgttggcg tggcgcggcg ccgattcaac gcgcgattga
     8701 agccggcgat gccgagacag gcgtgtgtat tatgcagatg gacatcggtt tggacaccgg
     8761 cgatgtggtc agcgaacacc gctacgccat ccaaccgacc gataccgcca acgaagtcca
     8821 cgacgcgctg atggaaatcg gtgcggcggc ggttgttgcc gatttgcaac agcttcaaag
     8881 caaaggccgt ctgaacgcgg tcaaacagcc cgaagaaggt gttacttacg cccaaaaact
     8941 aagcaaagaa gaggcgcgta tcgattggag cgaaagcgcg gcggttatcg aacgcaaaat
     9001 ccgcgccttc aaccccgtgc ctgccgcgtg ggttgagtat cagggcaagc cgatgaaaat
     9061 ccggcgggcg gaagtggtgg cgcaacaagg cgcggcaggc gaagtgttgt cctgttcggc
     9121 ggacggtttg gtcgttgcct gcggcgaaaa cgcgctgaag attaccgaat tgcagcctgc
     9181 cggcggcagg cgtatgaata tcgcggcgtt tgcagcagga cggcatatcg aagcagggac
     9241 gaagctgtaa atcccttcag acggcattcc gatccgcaaa tgggaatgcc gtctgaagtc
     9301 atcagttgaa gaaagcgaat cacataatat gagtatggca cttgcccaaa aacttgccgc
     9361 cgacagcatt gcggcggttg ccgaaggacg caaccttcag gacgtgttgg cgcaaatccg
     9421 caccgcgcat cccgacctta cggcgcagga aaacggcgcg ttgcaggaca tcgcctacgg
     9481 ttgccagcgt tatttgggca gtttgaaaca tatgctcgcg cagatgctga aaaaaccgat
     9541 tgacaatccg cagctcgaaa gcctgctttt ggcggcgttg taccaactgc attacacgcg
     9601 caacgcgccg cacgccgtgg tcaatgaggc ggtggaaagc atagccaaaa tcgggcgcgg
     9661 gcagtaccgt tcgtttgcca acgcggtttt gcgccgcttt ttgcgcgaac gcgacaagct
     9721 cgcggcttcc tgtaaaaaag acgatgtggc gaaacacaac ctgccgctgt ggtgggtggc
     9781 ttacctgaaa aaccattatc cgaaacactg gcacaacatc gccgccgcgc tgcaatccca
     9841 tccgccgatg acgttgcgcg tcaaccgccg gcacggcaac gccgaaagct atttggaaaa
     9901 actagctgcg gaaggcattg cggctaaggc gttggacgaa tatgcggtta cgttggaaga
     9961 agccgtgccg gtaaaccgcc tgcccggttt ttcagacggc atcgtttcgg tacaggactt
    10021 cggcgcgcag caggcggcgt atctccttaa cccgaaagac ggcgaacgga ttttggacgc
    10081 gtgcgccgcg ccgggcggca agacggggca tatcttggaa ctggcggatt gccgcgttac
    10141 cgccttagac attgatgcag gccgtctgaa gcgtgtggag gacaatatcg cccgtttggg
    10201 ctttcagacg gcatcggcgg cgtgtgccga tgcgcgggat ttggcggcgt ggtatgatgg
    10261 aaagcctttc gacgcaatcc ttgccgacgt gccatgcacc gcttccggcg tggcgcggcg
    10321 caatcccgac gtgaaatggc tgcgccgtcc gaccgacgcg ctcaaaaccg cccgccagca
    10381 ggaagccctg ctggacgcac tttggcagac gctgaaacag ggcggcagaa tgctgcttgc
    10441 cacctgttcc gtgttcgtcg aagaaaacga cgggcaattg caaaaattcc tcaaccgcca
    10501 tgccgatgcc gaaccgatcg aatcgcgggt gcttttaccg aacaaacacc aagatggctt
    10561 ttattacgcg cttattcaaa agcattaaac aatggcttgt gctgctgccg atgctttccg
    10621 ttttgccgga cgcggcggcg gaggggatag atgtgagccg cgccgaagcg aggataaccg
    10681 acggcgggca gctttccatc agcagccgct tccaaaccga gctgcccgac cagctccaac
    10741 aggcgttgcg ccggggcgtg ccgctcaact ttaccttaag ctggcagctt tccgccccga
    10801 taatcgcttc ttatcggttt aaattggggc aactgattgg cgatgacgac aatattgact
    10861 acaaactgag tttccatccg ctgaccaacc gctaccgcgt taccgtcggc gcgttttcga
    10921 cagactacga caccttggat gcggcattgc gcgcgaccgg cgcggttgcc aactggaaag
    10981 tcctgaacaa aggcgcgctg tctggtgcgg aagcagggga aaccaaggcg gaaatccgcc
    11041 tgacgctgtc cacttcaaaa ctgcccaagc cttttcaaat caatgcattg acttctcaaa
    11101 actggcattt ggattcgggt tggaaacctc taaacatcat cgggaacaaa taatgcgccg
    11161 ttttctaccg atcgcagcca tatgcgccgt cgtcctgttg tacggactga cggcggcaac
    11221 cggcagcacc agttcgctgg cggattattt ctggtggata gttgcgttca gcgcaatgct
    11281 gctgctggtg ttgtccgccg ttttggcacg ttatgtcata ttgctgttga aagacaggcg
    11341 cgacggcgta ttcggttcgc agattgccaa acgcctttct gggatgttta cgctggttgc
    11401 cgtactgccc ggcgtgtttc tgttcggcgt ttccgcacag tttatcaacg gcacgattaa
    11461 ttcgtggttc ggcaacgata cccacgaggc gcttgaacgc agcctcaatt tgagcaagtc
    11521 cgcattgaat ctggcggcag acaacgccct tggcaacgcc atccccgtgc agatagacct
    11581 catcggcgcg gcttccctgc ccggggatat gggcagggtg ctggaacatt acgccggcag
    11641 cggttttgcc cagcttgccc tgtacaatgc cgcaagcggc aaaatcgaaa aaagcatcaa
    11701 cccgcacaag ctcgatcagc cgtttccagg taaggcgcgt tgggaaaaaa tccaacgggc
    11761 gggttcggtc agggatttgg aaagcatagg cggcgtattg tacgcgcagg gctggctgtc
    11821 ggcgggtacg cacaacgggc gcgattacgc cttgtttttc cgtcagccgg ttcccaaagg
    11881 cgtggcagag gatgccgtct taatcgaaaa ggcaagggcg aaatatgctg agttgagtta
    11941 cagcaaaaaa ggtttgcaga cctttttcct ggcaaccctg ctgattgcct cgctgctgtc
    12001 gatttttctt gcactggtca tggcactgta tttcgcccgc cgtttcgtcg aacccgtcct
    12061 atcgcttgcc gagggggcga aggcggtggc gcaaggcgat ttcagccaaa cccgtcctgt
    12121 tttacgcaat gacgagttcg ggcgtttgac caagttgttc aaccacatga ccgagcagct
    12181 ttccatcgcc aaagaagcag acgagcgcaa ccgccggcgc gaggaagccg ccagacatta
    12241 tcttgaatgc gtgttggagg ggctgaccac gggcgtggtg gtgtttgacg aacaaggctg
    12301 tctgaaaacc ttcaacaaag cggcggaaca gattttgggg ttgccgctta cccccctgtg
    12361 gggcagcagc cggcacggtt ggcacggcgt ttcggcgcag cagtccctgc ttgccgaagt
    12421 gtttgccgcc atcggcgcgg cggcaggtac ggacaaaccg gtccatgtga aatatgccgc
    12481 gccggacgat gccaaaatcc tgctgggcaa ggcaaccgtc ctgcccgaag acaacggcaa
    12541 cggcgtggtg atggtgattg acgacatcac cgtgctgata cacgcgcaaa aagaagccgc
    12601 gtggggcgaa gtggcgaagc ggctggcaca cgaaatccgc aatccgctca cgcccatcca
    12661 gctttccgcc gaacggctgg cgtggaaatt gggcgggaag ctggatgagc aggatgcgca
    12721 aatcctgacg cgttcgaccg acaccatcgt caaacaggtg gcggcattga aggaaatggt
    12781 cgaagcattc cgcaattatg cgcgttcccc ttcgctcaaa ttggaaaatc aggatttgaa
    12841 cgccttaatc ggcgatgtgt tggcattgta tgaagccggt ccgtgccggt ttgcggcgga
    12901 gcttgccggc gaaccgctga tggtggcggc ggatacgacc gccatgcggc aggtgctgca
    12961 caatattttc aaaaatgccg ccgaagcggc ggaagaagcc gatgtacccg aagtcagggt
    13021 aaaatcggaa gcggggcagg acggtcggat tgtcctgacg gtttgcgaca acggcaaagg
    13081 gttcggcagg gaaatgctgc acaacgcctt cgagccgtat gtaacggaca aaccggcggg
    13141 aacgggattg ggtctgcctg tggtgaaaaa aatcattgaa gaacacggcg gccgcatcag
    13201 tttgagcaat caggatgcgg gtggcgcgtg tgtcagaata atcttgccaa aaacggtaaa
    13261 aacttatgcg tagcagcgat attttaattg tagacgacga aatcggcatc cgcgacctgc
    13321 tgtcggaaat cctgcaggac gaaggttatt cggtcgcatt ggcggaaaac gccgaagagg
    13381 cgcgcaagct gcgccatcag gcgcgccccg cgatggtgct gctggatatt tggatgcctg
    13441 attgcgacgg catcaccctt ttgaaggagt gggcgaaaaa cgggcagctc aatatgccgg
    13501 tggtgatgat gagcgggcat gccagcatcg ataccgccgt ggaagccacc aaaatcggtg
    13561 cgctcgactt tttggaaaaa ccgatttccc tgcaaaagct gctgtccacg gttgaaaacg
    13621 cgttgaagta cggtgcggcg caaaccgaaa cggggcctgt attcgacaag ctgggcaaca
    13681 gtgccgcgat tcaggagatg aactgcgaag tcgaagcggc ggcaaagcgc acgtcccctg
    13741 tatttttgac gggcgaagcg ggttcgccgt ttgaaaccgt ggcgcgctat ttccataaaa
    13801 acggtacgcc gtgggtcagt ccggcaaggg tcgaatatct gatcgatatg ccgatggaac
    13861 tgttgcagaa ggcggagggc ggcgttttgt atgtcggcga catctcccag tacagccgca
    13921 acatccaagc cggtattgcc tttattgtcg gaaaggcgga acaccgccgc gtcagggtgg
    13981 tcgcatcggg cagcagggcg gcaggttcag acggtattgc ctgcgaggaa aagctggcgg
    14041 aactgctgtc ggagtcggtc gtccgcattc cgccgctgcg tatgcagcat gaagatattc
    14101 ccttcctgat acaggggatt gcctgcaatg tggcggaaag tcaaaagatt gcgcccgccg
    14161 cattcagtga agaggcactt gccgcattga cccgttacga ctggccggga aatttcgacc
    14221 aactgcaaag cgtcgttgca acgctgttgt tggaggcgga cggacaggaa atcggcgcgg
    14281 cggcggtttc ttcccttttg gggcagaatg tgcctgccga gggagcggaa gatatggtgg
    14341 gcgggtttaa tttcaacctg cccctgcgcg aattgaggga ggaggtggag cggcgttatt
    14401 tcgagtacca catcgcccaa gaaggtcaga atatgagcaa agtggcgcag aaagtcggtt
    14461 tggaacgcac gcacctttac cgcaaactca aacagctcgg catcggcgtt tcgcgccggg
    14521 cgggggaaaa aaccgaagaa taggcccgga cggccggttt tccggctgcg ggcttttgtt
    14581 ttcagacggc atttggtgca aatgccgtct gaaatcgtaa ggggacggat tttatgacag
    14641 aggacgaacg tttcgcgtgg ctgcaattgg cgtttacgcc ctatatcggc gcggaaagct
    14701 tcctgctgct gctgcgccgt ttcggcagcg cgtcaaacgc cctgtccgca ccggcggaac
    14761 aggtcgcccc ggcggtgcgg cacaagcagg cggccgaggc ttggcgcaat gcggaaaaac
    14821 gcgcctcggc gcggcaggcg gcagaagcgg cgttggaatg ggaaatgcgg gacggatgcc
    14881 gtctgatgct gcttcaggat gaagatttcc ccgaaatgct gacgcagggg ctgaccgcgc
    14941 cgccggtttt gtttttgcgc ggcaacgtgc ggctgctgca caagccttcc gccgccatcg
    15001 tcggcagccg ccatgccacg ccgcaggcga tgcggattgc caaagatttc ggtaggacgt
    15061 tgtctgggaa aggcattccc gttgtgtcgg gtatggcttc gggcatcgat accgccgccc
    15121 atcagggtgc gttgcaggca gaaggcggca ccatcgccgt gtgggggacg ggcatagacc
    15181 gcatttatcc gccgtccaat aaaaaccttg cctatgaaat cgccgaaaaa gggctgattg
    15241 tcagcgagtt ccccatcggc acgcgcccgt atgccggcaa ctttccgcgc cgcaaccgcc
    15301 tgattgccgc gctgtcgcag gttacgctgg tggttgaagc tgcgttggaa tccggttcgc
    15361 tgattaccgc cggcctggcg gcagaaatgg ggcgcgaagt gatggcggta cccggttcga
    15421 tagacagtcc acacagcaaa ggctgccaca aactgattaa agacggcgca aaattggtgg
    15481 aatgcctgga cgacatcctg aacgaatgcc cggggctatt gcaaaatacg ggtgcttcat
    15541 catattctat aaataaggat acgcccgaca cgggacgccg cgccgttcag acggcatacg
    15601 ccccgccgcc tgccgcaaaa atgccgtctg aagcggcggc aggcggcaca gcccacggcg
    15661 gcatactgga taaaatgggt ttcgaccccg tccatcccga cgtgcttgcc gagcagcttg
    15721 ctatgcctgc cgcagatttg tatgccgcac tgttggaatt ggaattggac ggcagcgttg
    15781 ccgcaatgcc cggcggcaga taccagcgta tccgaacttg aacgcacttt atattaagga
    15841 acacgaatga ccgaagtcat cgcctacctc atcgaacatt tccaagattt cgatacctgt
    15901 ccgccgcccg aagacttggg tatgctgctt gaagaagcgg gtttcgatac gatggaaatc
    15961 ggcaacaccc tgatgatgat ggaagtattg ctcaacagct ccgaattttc cgccgaaccc
    16021 gccgacagcg gcgcattgcg cgtgtacagc aaagaagaaa ccgacaacct gccgcaggaa
    16081 gtgatggggc tgatgcagta tctgattgaa gaaaaagccg tcagctgcga acagcgggaa
    16141 atcatcatcc acgcgctcat gcacattccg ggcgacgaaa ttaccgtaga taccgccaaa
    16201 gtgctgaccc tgctgctttt atgggcaaac aagagcgagc tgcccgtgtt ggtcggcgac
    16261 gagctgatga gcgcgctttt actcgacaac aaacccacga tgaactgaag cggcttcaga
    16321 cggcccgccc gaggccgtct gaaacgccgg catcaaaacc accatccaga gaacgacaaa
    16381 tggcgaaaaa cctattaatc gtcgaatccc cgtccaaagc caaaaccctg aaaaaatatt
    16441 tgggcggcga ttttgaaatc cttgcatcct acggacacgt ccgcgacctc gtccccaaaa
    16501 gcggcgcggt cgatcccgac aacggctttg cgatgaaata ccaactcatc agccgcaacg
    16561 gcaaacacgt cgatgccatc gtcgccggtg ccaaagaagc tgaaaacatc tacctcgcca
    16621 ccgacccgga tagggaaggc gaagccattt cctggcatct tttggaaatc ctcaaatcca
    16681 aacgcggctt gaaaaacatc aagccgcagc gtgtcgtgtt ccacgaaatc accaaaaacg
    16741 ccgtgctcga tgccgttgcc catccgcgcg aaatcgaaat ggacttggtc gatgcgcaac
    16801 aagcccgccg tgctttggac tatttggtcg gctttaacct ttcgccattg ttatggaaaa
    16861 aaatccgccg aggtttgagc gcggggcgtg tgcaaagccc tgctttgcgc ctgatttgcg
    16921 aacgcgaaaa cgaaatccgc gcgtttgaag cgcaggaata ttggacggtg catctcgaca
    16981 gccacaaagg ccgcagcaag ttcaccgcca aactcgccca atacaacggc gcgaaactcg
    17041 aacaattcga cctgccgaac gaagccgcgc aagccgacgt attgaaagaa ctcgaaggca
    17101 aagaagccgt cgttaccgcc attgaaaaga aaaagcgcag ccgcaatcct gccgcgccgt
    17161 tcaccacttc taccatgcag caggatgccg tgcgcaaact cggcttcacc accgaccgca
    17221 ccatgcgtac cgcccaacag ctttatgaag gtatagacgt agggcagggc gcgatcggtc
    17281 tgattaccta tatgcgtacc gacagcgtga atttggcgga tgaagcgtta accgaaatcc
    17341 gccattacat tgaaaacaaa atcggcaaag aatatctgcc gagtgccgcc aaacaataca
    17401 aaaccaaatc caaaaacgcc caagaagccc acgaagccat ccgtccgact tccgtgtacc
    17461 gcacgcccga aagcgtcaaa cccttcctga gcgcagacca gttcaaactc tatcaaatga
    17521 tttggcagcg caccgtcgcc tgtcagatga cgcctgccaa attcgaccaa accaccgtcg
    17581 atattaccgt cggcaaaggc gtattccgcg taaccggaca agtgcaaacc ttcgcaggct
    17641 ttttgagcgt gtatgaagaa agcagcgatg acgaagaagg cgaagacagc aaaaaactgc
    17701 ccgaaatgag cgaaggcgat aaattgcccg tggacaaact ctacggcgaa caacacttta
    17761 ccactccgcc gccacgctac aacgaagcca cgctggttaa agccctcgaa gaatacggca
    17821 tcggccgccc ctcgacctac gccagcatca tctccacgct caaagaccgc gaatacgtta
    17881 cccttgagca aaaacgcttc atgcccaccg acacaggcga catcgtcaac aaattcctga
    17941 ccgaacactt cgcccaatac gtcgattacc actttaccgc caaactcgaa gaccagcttg
    18001 acgaaatcgc cggcggcaaa cgccaatgga ttcccttgat ggacaaattc tggaaaccgt
    18061 tcatcaaaca agtggaagaa aaagaaggca tcgaacgcgc caaatttacc acgcaggaac
    18121 ttgatgaaac ctgcccgaaa tgcggcgaac acaaactgca aatcaagttc ggcaaaatgg
    18181 gccgtttcgt tgcgtgtgcc ggctatcccg agtgcagcta cacgcgcaat gtcaatgaaa
    18241 ccgccgaaga agccgcagag cgcatcgcca aagccgaagc cgaacaggca gaactcgacg
    18301 gacgcgaatg tcctaaatgt ggtggtcgcc tagtgtacaa atacagccga accggcagca
    18361 aattcatcgg ctgcgccaac tatccgaaat gcaaacacgt cgagccgctg gaaaaaccga
    18421 aagataccgg cgtccagtgt ccgcaatgca aaaaaggcaa cctcgtcgag cgcaaatccc
    18481 gctacggcaa actgttttac agctgcagca cctatcccga ctgcaactac gccacctgga
    18541 acccgcccgt cgccgaagaa tgcccgaact gccattggcc ggtcttgacc atcaaaacca
    18601 ccaaacgctg gggcgtcgaa aaagtctgcc cgcaaaaaga atgcggctgg aaagaacaga
    18661 ttgaaccgcc cgcgccgaag gagtaagatt aggttggttt gaaagagaaa aggtcgtctg
    18721 aaaaattttc agacgacctt tgcttttctg tgattggttt atttgaatcc gcgtgttgtt
    18781 ttaaagtccg ataaaatccg gttcatttca ggcacaaaca aggcgatgta atcgtaagat
    18841 agaccgcgac tggcactggg atggggaaag cagacgactt cgcaatcttc aaacgattgg
    18901 aatttgacat tgaaacgtgt accgtcaaat tctttttgca ccgtctccag cggtttggtc
    18961 tgcttaccga ccaactgctc gaagcgtggc agtacatttt ggttgttcag aaaatccgcc
    19021 aacctgctgc ccatgaagag gatgactttc ggacgcagtt tttcgatgtg gtagagaaaa
    19081 ttatcgatgt gctcgggttg tgtgaacttg tcgggattgt cgatagtgtt gccctgtgta
    19141 gcagcccagt tggtttgaac cagggatttt tcaaatgcac cgcccaatcc attttcgtct
    19201 aaggggtgtc cccacatttc aaaccaattt tttatcgtat tgtcgtaacg ccactttttt
    19261 gcctgctctc cgaaatagag ggatttgttt gcaaatgtat ggtcgatttt gttttcaggg
    19321 agtttgtatt cacctgctac ataagcagcc tcatcggctt tactccaacc ccattcatag
    19381 ccacaaatca ttaagccatg tttgtcgttg tagcctttga acaggctgtt gctcaaattc
    19441 aaatccttca tcatgaactc ttccttttaa aatttaagag cgattgactt caatgttttt
    19501 agatggggtg gaaaaatcct tgtgtaggca acataaattc aataaatttc ttgataattc
    19561 gaaacctact aatagcgcac ctataaaagc tttttcatta cgttcagcat gacggtcacg
    19621 tcgttcatat tttttacgct tgctgttccc tgttattaca gctaagccaa gtgatatggc
    19681 gagaattgcc caaacaatag tacttaataa caattttccc catactatca atagggaaag
    19741 aaaaaacctt ttagtattag gtcgatagat tataatccat gcccatgaaa atgcaataag
    19801 agttatacat aagatacaag ctgataggat ttctttattt ttaattaaat aacttagggc
    19861 gatgaggatg acaggtgtca agaaaacaat agttacatcc cgatagctat aaaagaaaac
    19921 tgccccattt tgaatgtgga aatgtgcaca gaatccaata tagctaagga taatagttaa
    19981 tataataaaa aaagaccacc aagggtgaag agataggaat tccatgtttt cccttttttt
    20041 gtaaaaagga aaaaatctta tctaatagtt aattgcctaa ccagccagaa gaagtttaaa
    20101 atctatccca ataattcaac catctataca gaaagttcag cttatggaaa cccacgaaaa
    20161 aatccgcctg atgcgcgaat tgaataaatg gtcctaggag gatatggcgg aaaagctggc
    20221 gatgtcggca ggcgggtatg ccaaaatcga acgaggcgaa acgcagttga atatcccgcg
    20281 tttggagcag ttggcgcaga ttttcaaaat tgatatgtgg gacttgctca aatcgggcgg
    20341 cggcgggatg gtgttgcaga ttaatgaagg tgatagtggt ggcgatattg cgttgtatgc
    20401 gtcgggtgat gtttcgatga aaatagaatt tttaaaaatg gagttgaaac actgcaaaga
    20461 aatgttggaa caaaaagaca aagaaatcga gctgctccgc aagctgaccg aaaccgttta
    20521 aacagatatg ccgtctgaaa aaagttttca gacggcatat tctttgacag gtcttgtata
    20581 ataccgtttg aacttacagg tttttgatta tggcggcagg caaacatacc aaacacagca
    20641 accgggtacg cattatcggc gggcaatgcc ggggcaggaa attgagtttc acatccgccg
    20701 acggactgcg tccgacaccc gacagcgtgc gtgaaaagct gtttaactgg ctgggacagg
    20761 atttgacggg taaaacggtt ttggatctct tcggaggcag cggcgcactc ggtatagaag
    20821 ccgcttcgcg caacgccaaa cgcgtgctga tttcggataa caaccgccaa accgtgcaga
    20881 ccttgcagaa aaacagtcgc gaactgggtt tggggcaggt gcaaatcgtc ttttcagacg
    20941 gcatcgcata tttgaagacc gtatccgaac agtttgatgt tgtctttctc gacccgccgt
    21001 ttgcatggca ggactggcaa atcctgtttg atgccttgaa gccgtgcctg aacccccggg
    21061 cattcgtcta tctcgaggcg ggtacgctgc cgaatattcc cgattggctg acggaatata
    21121 gagaagggaa atcggggcag agtacatttg aattaagggt tttccaagtg gctgaataat
    21181 atgcgctttg ataatcattt ccgagttgta aacattcgtt tgcaaccgtc cggttcaaaa
    21241 aaaccttgtg ctataatccg cgcccgcccg gttttgataa tttagtggaa aaggaaaaga
    21301 aatgtcgctt tttattaccg acgagtgcat caactgcgac gtatgcgaac ccgaatgccc
    21361 caatgatgcc atttcccaag gcgaggaaat ttacgaaatc aaccccaacc tctgcacgca
    21421 gtgcgtcgga cactacgatg agccgcagtg ccagcaggtt tgcccggtgg actgcatcct
    21481 gattgaccaa gaacatcccg aaacccatga cgagttgatg gcgaaatacg aaaagattat
    21541 ccagtttaaa taaattcttt ttaaaacatc aaattatgtc tgttttgaaa taaaatcaaa
    21601 aaaaaacttg acggaaaagc aagccgctaa taaactaacg ttctcttttg gagggattcc
    21661 cgagcggtca aagggggcag actgtaaatc tgttgcgaaa gcttcgaagg ttcgaatcct
    21721 tctccctcca ccaaaattct tacttggggc agtagcgagt aatgcgggtg tagctcaatg
    21781 gtagagcaga agccttccaa gcttacggtg agggttcgat tcccttcacc cgctccaaac
    21841 aattaggccc atgtagctca ggggtagagc actcccttgg taagggagag gtcggcagtt
    21901 caaatctgcc catgggcacc atccctcgat tattcatttc tttaaggctt agatatatag
    21961 gatattg
//
LOCUS       DAWFHY010000030        21286 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.030, whole genome shotgun sequence.
ACCESSION   DAWFHY010000030 DAWFHY010000000
VERSION     DAWFHY010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21286
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.030"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(148..1590)
                     /gene="aldA"
                     /locus_tag="WGQ68_000974"
     CDS             complement(148..1590)
                     /gene="aldA"
                     /locus_tag="WGQ68_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEZ0714797.1"
                     /translation="MKQLAMYINGRFENDFNGEWRDVLNPSTEEAIASEPKGGKADVD
                     RAVAAARAAQPAWERLPAVERGAYLRKIAQGIRERADELTDTIVAEGGKTKDLARVEV
                     MFTADYLDYQAEWARRYEGEIIQSDRPRENILLFKRPLGVIAGILPWNFPFFLIARKM
                     GPALVTGNTIVVKPSSVTPINCHIFAEIVDAVGLPAGVFNVVNGPGAEIGNALSAHPQ
                     VDMVSLTGSVEAGRQVMEAASANITKVSLELGGKAPAIVLKDADLDLAVKSILASRVG
                     NTGQICNCAERVYVHSSLKDAFIEKMTAAMKGVRYGNPAEAEAGALEMGPLIEERAVK
                     AVAEKVERAVKQGAKLVCGGKRAEGRGYFFEPTLLTDTDNSMDIMKEETFGPVLPVSA
                     FDTLDQVIALANDCEFGLTSSVYTTNLNEAFYVTRRLQFGETYINRENFEAMQGFHAG
                     WKKSGIGGADGKHGLEEYLQTQVVYLETDI"
     gene            complement(1698..2615)
                     /locus_tag="WGQ68_000975"
     CDS             complement(1698..2615)
                     /locus_tag="WGQ68_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0714798.1"
                     /translation="MEKAEHLNSSRFVNLVKSGGGSYVEGSYRFDTLSNGISIHGGTV
                     TARCDFCSSRLAEPYVSFVLLLEGSLDFGINRCRFQIDADGGKIVLIAVGEEVLFSRY
                     LYRGGKTVKMTIKGMEQWLLRPEYARFAPLLYREPVRIWDLPPNLRGLAASCLKAVPK
                     GHLGKTLRREADVLRLLSDLWDTVSDGIGPAAGQTAEADAMPSEDFSRTLNAAFADGA
                     HQVNRLTDALNISERTLQRRMRDHFGITASEWLHHKQMQHALYLLQNGGKSIGETAYL
                     CGYRHVSSFTQAFRQYFGSTPAETKKENR"
     gene            2788..3588
                     /locus_tag="WGQ68_000976"
     CDS             2788..3588
                     /locus_tag="WGQ68_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714799.1"
                     /translation="MSPSPFIEMKDVAFAYGDRPILKNINFSIPQGNFAAVMGGSGSG
                     KTTLMRLITGQIRPQSGQVLIEGRDLAGFSADELYEHRRRMGVLFQHGALFTDLSVFD
                     NIAFPMRELTRLPEAVIRDLVLLKLNAVGLRGVENLMPSELSGGMSRRVALARTIALD
                     PEIMLYDEPFTGLDPISLGVIAHLISRVNKALRSTSIMVTHDIEKSLEIVDQVIFLAH
                     GEIMFSGSPQEMRELDSPWVRQFVGGLADGPVAYRYPAQTSLQQDLLG"
     gene            3636..4412
                     /gene="mlaE"
                     /locus_tag="WGQ68_000977"
     CDS             3636..4412
                     /gene="mlaE"
                     /locus_tag="WGQ68_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HEZ0714800.1"
                     /translation="MNFIRSVGAKTLGLIQSLGSITLFLLNILAKSGTAFVRPRLSVR
                     QVYFAGVLSVLIVAVSGLFVGMVLGLQGYTQLSKFKSADILGYMVAASLLRELGPVLA
                     AILFASSAGGAMTSEIGLMKTTEQLEAMNVMAVNPVARVVAPRFWAGVFSMPLLASIF
                     NVAGIFGAYLVGVSWLSLDSGIFWSQMQNNITIHYDVINGLIKSAAFGVAVTLIAVHQ
                     GFHCVPTSEGILRASTRTVVSSALTILAVDFILTAWMFTD"
     gene            4463..4957
                     /gene="mlaD"
                     /locus_tag="WGQ68_000978"
     CDS             4463..4957
                     /gene="mlaD"
                     /locus_tag="WGQ68_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HEZ0714801.1"
                     /translation="MKKNILEFWVGLFVLIGAAAVAFLAFRVAGGAAFGNSGKTYTVY
                     ADFGDIGGLKVNAPVKSAGVLVGRVGAIGLDPKSYQARVRLDLDGKYQFSSDVSAQIL
                     TSGLLGEQYIGLQQGGDTENLAAGDTISVTSSAMVLENLIGKFMTSFAEKNADGGNAE
                     KAAE"
     gene            4994..5584
                     /locus_tag="WGQ68_000979"
     CDS             4994..5584
                     /locus_tag="WGQ68_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HEZ0714802.1"
                     /translation="MKKSSLISALGIGILSIGMAFAAPADAVNQIRQNATQVLSILKS
                     GDANTARQKAEAYAIPYFDFQRMTALAVGNPWRTASDAQKQALTKEFQTLLIRTYSGT
                     MLKLKNANVNVKDNPIVNKGGKEIIVRAEVGVPGQKPVNMDFTTYQSGGKYRTYNVAI
                     EGASLVTVYRNQFGEIIKAKGVDGLIAELKAKNGSK"
     gene            5641..5919
                     /locus_tag="WGQ68_000980"
     CDS             5641..5919
                     /locus_tag="WGQ68_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HEZ0714803.1"
                     /translation="MHTELNSGTLHISGDITVKTLTGEAFERFQQQCRLKETIAVDFG
                     GVKRADSACVSLLLEVLRGCKGSVRLTGIPESVRALSELYEIKDWLKS"
     gene            5916..6743
                     /locus_tag="WGQ68_000981"
     CDS             5916..6743
                     /locus_tag="WGQ68_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HEZ0714804.1"
                     /translation="MKKTAYAFLLLIGFASAPAFAETRPADPYEGYNRAVFKFNDQAD
                     RYIFAPAARGYRKVAPKPVRAGVSNFFNNLCDVVSFGSNILRLDIKRASEDLVRVGIN
                     TTFGLGGLIDIAGAGGIPDNKNTLGDTFASWGWKNSNYFVLPVLGPSTVRDALGTGIT
                     SVYSPKNIVFRTPVGRWGTTAVSAVSTREGLLDLTDSLDEAAIDKYSYTRDLYMKVRA
                     RQTGATPAEGTEDNIDIDELVESAETGAAETAVQEDSVSETQAEAAGEAETQPGTQP"
     gene            6868..7230
                     /locus_tag="WGQ68_000982"
     CDS             6868..7230
                     /locus_tag="WGQ68_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ"
                     /protein_id="HEZ0714805.1"
                     /translation="MYEVNRSVFVLIPLEPFWNWLQTLPGNHLDGLTLEDIQADANSY
                     LVRPCETADEVWDEIEARFEDIFAAELADWCEDEREWPALDADIFNEWFDIQLSTVIT
                     DLEHEPLAREAFQPINLN"
     gene            7227..7613
                     /locus_tag="WGQ68_000983"
     CDS             7227..7613
                     /locus_tag="WGQ68_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004284370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEZ0714806.1"
                     /translation="MMKLTVRNYHLDGYGHVNNARYLEFFEEARWAFFEERGLLHELA
                     GLILIVARIDIRYSRPAVEGDVLQFSCRLKTPGTRRIVLTQTITLPNGKTAAEADITL
                     MPVHAATQRTVSLPATLARALEALSE"
     gene            7610..8098
                     /locus_tag="WGQ68_000984"
     CDS             7610..8098
                     /locus_tag="WGQ68_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0714807.1"
                     /translation="MKKILTAAVVALIGILLAIVLIPDSKTAPAFSLSDLHGKTVSNA
                     DLQGKVTLINFWFPSCPGCVSEMPKIIKTANDYKNKNFQVLAVAQPIDPIESVRQYVK
                     DYGLPFTVMYDADKAVGQAFGTQVYPTSVLIGKKGEILKTYVGEPDFGKLYQEIDTAL
                     AQ"
     gene            complement(8179..8346)
                     /locus_tag="WGQ68_000985"
     CDS             complement(8179..8346)
                     /locus_tag="WGQ68_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEZ0714808.1"
                     /translation="MEDDVWPGHRVIVMAGVTVGRGSVVGAGAVVTKDIPPYSLAAGN
                     PAVVKKNLPEG"
     gene            complement(8313..8687)
                     /locus_tag="WGQ68_000986"
     CDS             complement(8313..8687)
                     /locus_tag="WGQ68_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-o"
                     /protein_id="HEZ0714809.1"
                     /translation="MGSKFFFLLLRFAGSVLPPSHMRGIVGRRVRGFLARRVSPHIGR
                     GVNIERGAYVFPDTVLGDGSGIGANCEICRGLVVGKNVMMEPECLFYSNNHKFDRSKN
                     ALRATRKSVRLRWRTMSGRGTG"
     gene            8697..8800
                     /locus_tag="WGQ68_000987"
                     /pseudo
     CDS             8697..8800
                     /locus_tag="WGQ68_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8925..9140
                     /gene="rpmE"
                     /locus_tag="WGQ68_000988"
     CDS             8925..9140
                     /gene="rpmE"
                     /locus_tag="WGQ68_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003681847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEZ0714810.1"
                     /translation="MKQGIHPNYHEVNVTCSCGNKFATKSAMEKENFNIEVCSLCHPF
                     YTGTQKIVDTTGRVDKFNNKFGNLFKR"
     gene            9456..10085
                     /locus_tag="WGQ68_000989"
     CDS             9456..10085
                     /locus_tag="WGQ68_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEZ0714811.1"
                     /translation="MIQNVVTSIILYFGTAVDLLIILMLFFAKRKSRKDIINIYLGQF
                     LGSVSLILLSLLFAFVLDYIPSKEILGLLGLIPIFLGIKVLLLGDSDGEAIAKEGLRK
                     DNRNLIFLVAMITFASCGADNIGVFVPYFTTLNSANLIVALLTFLVMIYLLVFSAQKL
                     AQVPSVGETLEKYSRWFVAVVYLGLGIYILIGNNSFDMLWTVSGQEKIL"
     gene            10231..11907
                     /locus_tag="WGQ68_000990"
     CDS             10231..11907
                     /locus_tag="WGQ68_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEZ0714812.1"
                     /translation="MLTYTPSDARTPAKTHEKPWLLMLMAFAWLWPGVFSHDLWNPAE
                     PAVYTAVEALAGSPTPLVAHLFGQTDFGIPPVYLWVAAAFKHLLSPWAADPYDAARFA
                     GVFFAVVGLTSCGFAGFNFLGRHHGRSVVLILIGCIGLIPVAHFLNPTAAAFAAAGLV
                     LHGYSLARRRVIAASFLLGTGWTLMSLAAAYPAAFALMLPLPVLMFFRPWQSRRLMLT
                     AVASLAFALPLMTVYPLLLAKTQPALFAQWLDDHVFGTFGGVRHVQTAFSLFYYLKNL
                     LWFALPALPLAVWTVCRTRLFSTDWGILGVVWMLAVLVLLAVNPQRFQDNLVWLLPPL
                     ALFGAAQLDSLRRGAAAFVNWFGIMAFGLFAVFLWTGFFAMNYGWPAKLAERAAYFSP
                     YYVPDIDPIPMAVAVLFTPLWLWAITRKNIRGRQAVTNWAAGVTLTWALLMTLFLPWL
                     DAAKSHAPVVRSMEASLSPELKRELSDGIECIDIGGGDLHTRIVWTQYGTLPHRVGDV
                     QCRYRIVRLPQNADASQGWQTVWQGARPRNKDSKFALIRKIGENILKTTD"
     gene            12009..12614
                     /locus_tag="WGQ68_000991"
     CDS             12009..12614
                     /locus_tag="WGQ68_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0714813.1"
                     /translation="MMTLYSGITCPFSHRCRFVLYEKGMDFEIKDVDIYNKPEDLAVM
                     NPYNQVPVLVERDLVLHESNIINEYIDERFPHPQLMPGDPVMRGRGRLVLYRMEKELF
                     NHVQVLENPAATNKEQAKAREAIGNGLTMLAPSFSKSKYILGEDFSMIDVALAPLLWR
                     LGHYDVKLGKSAAPLLKYAERIFQREAFIDALTPAEKAMRK"
     gene            12686..13078
                     /locus_tag="WGQ68_000992"
     CDS             12686..13078
                     /locus_tag="WGQ68_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HEZ0714814.1"
                     /translation="MPTSTKPYILRALCEWCSDNSLTPHILVWVNEHTRVPMQYVRDN
                     EIMLNIGATATQNLQIDNDWISFSARFGGQAHDIWIPVGHVLSLFARETGEGMGFELE
                     AYRPDTPPENTSAETAPRPAKKGLKLVK"
     gene            complement(13107..13256)
                     /locus_tag="WGQ68_000993"
     CDS             complement(13107..13256)
                     /locus_tag="WGQ68_000993"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714815.1"
                     /translation="MPSEARIAGSDGIFNVPISNRQGQHGFDTPKRVLPINGFRLRPI
                     GQNAV"
     gene            complement(13277..13723)
                     /locus_tag="WGQ68_000994"
     CDS             complement(13277..13723)
                     /locus_tag="WGQ68_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEZ0714816.1"
                     /translation="MSLKIRLTEDMKTAMRAKDQVSLGTIRLINAAVKQFEVDERTEA
                     DDAKITAILTKMVKQRKDSAKIYTEAGRQDLADKENAEIEVLHRYLPQMLSAGEIRTE
                     VEAAVAETGAAGMADMGKVMGVLKTRLAGKADMGEVNKILKAVLTA"
     gene            complement(13855..14067)
                     /locus_tag="WGQ68_000995"
     CDS             complement(13855..14067)
                     /locus_tag="WGQ68_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEZ0714817.1"
                     /translation="MPAIRVKENEPFEVAMRRFKRAVEKTGLLTELRAREAYEKPTTE
                     RKRKKAAAVKRLQKRLRSQQLPPKMY"
     gene            complement(14356..16206)
                     /locus_tag="WGQ68_000996"
     CDS             complement(14356..16206)
                     /locus_tag="WGQ68_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0714818.1"
                     /translation="MYLPSMKHSLPLLAALVLAACSSTNTLPAGKTPADNIETADLSA
                     SVPTRPAEPEGKTLADYGGYPSALDAVKQKNDAAVAAYLENAGDSAMAENVRNEWLKS
                     LGARKQWTLFAQEYAKLEPAGRAQEVECYADSSRNDYTRAAELVKNTGKLPSGCTKLL
                     EQAAASGLLDGNDAWRRVRGLLAGRQTTDARNLAAALGSPFDGGTQGSREYALLNVIG
                     KEARKSPNAAALLSEMESGLSLEQRSFAWGVLGHYQSQNLNVPAALDYYGKVADRRQL
                     TDDQIEWYARAALRARRWDELASVISHMPEKLQKSPTWLYWLARSRAATGNTQEAEKL
                     YKQAAATGRNFYAVLAGEELGRKIDTRNNVPDADANSVLRMAEDGAIKRALVLFQNSR
                     TAGDAKMRRQAQAEWRFATRGFDEDKLLTAAQTAFDHGFYDMAVNSAERTDRKLNYTL
                     RYISPFKDTVIRHAQNVNVDPAWVYGLIRQESRFVIGAQSRVGAQGLMQVMPATAREI
                     AGKIGMDAAQLYTADGNIRMGTWYMADTKRRLQNNEVLATAGYNAGPGRARRWQADTP
                     LEGAVYAETIPFSETRDYVKKVMTNAAYYAALFGAPHIPLKQRMGIVPAR"
     gene            16583..17320
                     /locus_tag="WGQ68_000997"
     CDS             16583..17320
                     /locus_tag="WGQ68_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEZ0714819.1"
                     /translation="MIILDKVSKHYQTRDKTRFAAVEPTSLEIRDGEIFGLMGYSGAG
                     KSTLLRLINLLERPDSGKVNVCGQELTALDAAALRQARQNIGMVFQQFNLLNNRTVAG
                     NVAFPLEIAGWPSEKIKARVAECLEIVGLTERAGHYPAQLSGGQKQRVGIARALASKP
                     QVILADEPTSALDPATTRSVLECLEDINKRFNVTIVIVTHEMSVIRRLCDRAALLDKG
                     KVVEIVEVRGNQIHARSDIGRELIRED"
     gene            17322..18008
                     /locus_tag="WGQ68_000998"
     CDS             17322..18008
                     /locus_tag="WGQ68_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0714820.1"
                     /translation="MADLTFQQAVSNIIGMKGEIVRALGETFVMVGLSTTFAVVFGTL
                     LGVLLFVTSSRQLHYNKPVNFLLDNLVNLMRAFPFVILMIAMIPATRAIVGSTIGPIA
                     ASLVLSVSGLFYFARLVEQNLREVPKGVIEAAAAMGAPPIAIVCKVLLNEARAGMVSS
                     ITVLAIGLLSYSAAAGMIGGGGLGDLAIRYGYYRYQTEVIVFIVALLVLLVILIQSTG
                     NALARKLDKR"
     gene            18210..19073
                     /locus_tag="WGQ68_000999"
     CDS             18210..19073
                     /locus_tag="WGQ68_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714821.1"
                     /translation="MKTFFKTLSAAALALILAACGGQKDSAPAASASAAADNGAAKKE
                     IVFGTTVGDFGDMVKEQIQPELEKKGYTVKLVEFTDYVRPNLALAEGELDINVFQHKP
                     YLDDFKKEHNLDITEVFQVPTAPLGLYPGKLKSLEEVKDGSTVSAPNDPSNFARVLVM
                     LDELGWIKLKDGINPLTASKADIAENLKNIKIVELEAAQLPRSRADVDFAVVNGNYAI
                     SSGMKLTEALFQEPSFAYVNWSAVKTADKDSQWLKDVTEAYNSDAFKAYAHKRFEGYK
                     SPAAWNEGAAK"
     gene            complement(19135..19704)
                     /locus_tag="WGQ68_001000"
     CDS             complement(19135..19704)
                     /locus_tag="WGQ68_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEZ0714822.1"
                     /translation="MVRRLIIGISGASGFQYGVKALELLRAQDIETHLVVSKGAEMAR
                     ASETAYARDEVYALADFVHPIGNIGACIASGTFKTDGMLVAPCSMRTLASVVHGFGDN
                     LLTRAADVVLKERRRLVLMVRETPLNLAHLDNMKRVTEMGGVVFPPVPAMYRKPQTAD
                     DIVAHSVAHALSLFGIDTPDSAEWQGMAD"
     gene            19862..20722
                     /locus_tag="WGQ68_001001"
     CDS             19862..20722
                     /locus_tag="WGQ68_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEZ0714823.1"
                     /translation="MAKVKGGLGRGLDSLLANGADNSSGDRLTTVAVKDIRPGRYQAR
                     VQIDDEALQELADSIKAQGVIQPVIVREHGLSRYELIAGERRWRAAQIAGLTEIPAVI
                     KTISDETALAMGLIENLQRENLNPIEEAQGLKRLADEFGLTHETIAQAVGKSRSAISN
                     SLRLLSLPEPVQEMLYQRRLEMGHARALLTLPVVEQLELAQKAVKNGWSVREVERRSQ
                     AALQNKRPEPKKTAAADIGRLNDLLTEKLGVNAEIKTANHKKGKIILHFDTPETFDHI
                     LKQLGIDYRP"
     gene            20857..21195
                     /locus_tag="WGQ68_001002"
     CDS             20857..21195
                     /locus_tag="WGQ68_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714824.1"
                     /translation="MNTRIIVSAAFVALALAGCGSINNVTVSDQKLQERAAFALGVSP
                     NAVKISNRSNEGIRINFTATVGKRVSQCYVTSVISTIGVTTSDAICLGGGTHKGKSQC
                     NALLKAAGRC"
BASE COUNT         4756 a         6009 c         5552 g         4969 t
ORIGIN      
        1 cgccttgtcc tgatttttgt taatccacta tacaaagaca aaaacatcga cctcggtcat
       61 gactgattgc cggtgaagca ataaaaatgc cgtctgaacc tgaaaacggg ttcagacggc
      121 attttctatc agggttttaa agcggcatta aatgtcggtt tccagataaa cgacttgggt
      181 ttgcagatat tcttccaaac cgtgtttgcc gtccgcgccg ccgataccgg attttttcca
      241 accggcgtgg aaaccctgca tcgcttcaaa gttttcgcgg ttgatgtagg tttcgccgaa
      301 ttgcaggcgg cgggtaacgt agaaggcttc gtttaaatta gtcgtataaa cagaactggt
      361 cagaccaaac tcgcaatcgt tagccagtgc aatcacttgg tcgagcgtgt cgaaagcgga
      421 aacgggcaga acggggccga aggtttcttc tttcataatg tccatactgt tgtcggtgtc
      481 ggtcagcagg gtaggctcga agaaatagcc gcgtccttcg gcgcgtttgc cgccgcaaac
      541 cagtttcgca ccttgtttga ctgcccgttc cactttttcg gcaacggctt tgacggcgcg
      601 ctcttcaatc agcgggccca tttccagcgc gccggcttcg gcttcggcag ggttgccgta
      661 gcgcacgcct ttcatcgcgg cggtcatttt ttcgataaac gcgtctttca ggctgctgtg
      721 gacatagacg cgctcggcgc agttgcagat ttgaccggtg ttgccgacgc gcgaagccaa
      781 gatggattta accgccaaat ccaaatccgc gtctttcaaa acgatggcgg gggctttgcc
      841 gccgagttcc agcgaaactt tggtgatgtt ggcggaagcg gcttccatca cttggcggcc
      901 tgcttcgacg gagccggtca ggctgaccat atcgacttgc ggatgggcgg acaaggcgtt
      961 gccgatttcc gcgccgggac cgttcaccac gttgaacacg ccggcgggca gtccgaccgc
     1021 atcgacgatt tcggcgaaga tgtggcagtt gatcggggtt acgctgctgg gtttgacgac
     1081 gatggtgttg cccgttacca aagcgggccc cattttgcgg gcaatcagga agaaggggaa
     1141 gttccacggc aaaatgccgg caatcacgcc cagcggacgt ttgaacaata aaatattttc
     1201 acgcgggcgg tcgctttgga tgatttcgcc ttcgtaacgg cgcgcccatt cggcttggta
     1261 atcgagatag tcggcggtaa acatgacttc cacgcgtgcc aagtctttgg ttttgccacc
     1321 ttcggcaacg atggtgtcgg tcagctcgtc ggcacgttcg cgtatgcctt gggcgatttt
     1381 gcgcaaatac gcgccgcgtt cgaccgcagg cagacgttcc caagccggtt gcgccgcacg
     1441 cgccgccgct acggcgcggt caacgtctgc tttgccgcct ttgggttcgc tggcgatggc
     1501 ctcttcggta gacgggttca atacgtcgcg ccattcgccg ttgaagtcgt tttcaaagcg
     1561 accgttgatg tacatggcca attgtttcat ttcgggttct ccagttttgt agtcagatgt
     1621 agttttagtt tattcccaaa taaattgccg tacaagtttc tttacacgcg gatttctggt
     1681 ttgaaatcaa atgcggctta ccggttttct tttttggttt ccgccggcgt gctgccgaaa
     1741 tattgcctga atgcctgagt aaagctggaa acgtggcggt agccgcacaa atacgcggtt
     1801 tcgcctatgc ttttgccccc gttttgcaac aggtagagcg cgtgctgcat ttgtttgtgg
     1861 tgcagccact cgcttgcggt aataccgaaa tggtcgcgca tacggcgttg cagcgtcctt
     1921 tcactgatgt tcagcgcgtc tgtcagccgg ttgacttggt gtgcgccgtc ggcaaacgcg
     1981 gcatttaggg tgcggctgaa gtcttcagac ggcatagcgt ctgcttccgc cgtttgcccc
     2041 gccgccggcc cgatgccgtc tgaaaccgtg tcccacaagt ccgacagcag ccgcaacacg
     2101 tccgcctcgc ggcgtaatgt tttgcccaaa tgcccctttg ggacggcttt caggcaggat
     2161 gccgccaagc cgcgcaggtt cgggggcaaa tcccatatcc tgaccggttc gcggtaaagc
     2221 aggggtgcga aacgcgcgta ttccggacgc agcagccatt gttccatacc tttaatggtc
     2281 attttgaccg ttttgccgcc tcggtaaaga tagcggctga acaggacttc ttccccgaca
     2341 gcaattagga caatcttgcc gccgtccgca tcgatttgga agcggcagcg gttgatgccg
     2401 aagtccaaac tgccttccag caagagcacg aacgacacat aaggttcggc gaggcggctg
     2461 ctgcaaaaat cacaccgtgc cgttactgtg ccgccgtgga tggaaatgcc gttggacaaa
     2521 gtatcgaaac ggtagctgcc ctccacatag ctgccgccgc cgcttttgac tagattgacg
     2581 aaccggctgc tgttcaaatg ttctgctttt tccatagttt tctgataaag gttatcattt
     2641 gaaagataac atttttcgcc acagcaaaca atccgccgcc gacaacggca ttttcacaac
     2701 atttaccgtc cgtatgccgt ctgaaaattg cccgtcttcc cgataagcct taaaatacac
     2761 gccgtcaaac ctaccgtccg tccgtacatg agcccatccc cctttatcga aatgaaagac
     2821 gtcgccttcg cgtatggcga ccgcccgatt ctgaagaaca tcaatttcag cattccgcaa
     2881 ggcaattttg ctgccgtaat gggcggttcg ggcagcggca aaaccacgct gatgcgactg
     2941 attacaggac agattcgtcc gcagtccggg caggttttga ttgaaggacg ggatttggcg
     3001 ggcttttcgg ctgacgagtt gtatgaacac cgccgccgta tgggcgtatt gttccaacac
     3061 ggcgcgctgt ttactgattt gtcggtattc gacaatatcg cttttccgat gcgcgaactg
     3121 acgcgcctgc cggaagcggt gattcgggat ttggttttgt tgaaattgaa cgcggtcggt
     3181 ctgcgcggtg tggaaaacct gatgccgtcc gagttgtccg gcgggatgtc gcgccgcgtc
     3241 gcgcttgccc gcacaatcgc gctcgaccct gaaatcatgt tgtacgacga gccgtttacc
     3301 ggcctcgatc cgatttcgct gggtgtgatt gcccacttaa tcagccgcgt caacaaggct
     3361 ttgcgttcga ccagtattat ggtaacgcac gacattgaaa aatctttgga aatcgtcgat
     3421 caggtgattt tcttggcgca cggcgaaatt atgttttccg gctcgccgca ggaaatgcgc
     3481 gaactggatt cgccttgggt gcgccagttt gtcggcgggc tggcagacgg ccccgtagca
     3541 taccgctatc cggcgcaaac gtcgttgcag caggatttgc tcgggtaaat gagcaggtgc
     3601 cgtctgaaac cgttgaactt tacatataga aatttatgaa ttttatccgt tccgtcgggg
     3661 cgaaaaccct cggccttatt caatccctcg gcagtattac gctgtttctg ctgaacattc
     3721 tggcgaagtc cggtacggct ttcgtccgtc cgcgcctgag cgtgcgccaa gtgtattttg
     3781 ccggcgtgct gtcggtgctg attgttgccg tttcggggct gtttgtcggt atggtcttgg
     3841 gtttgcaggg ctatacgcag ttgtcgaaat tcaaatccgc cgatattttg ggctatatgg
     3901 ttgcggcttc gctgttgcgc gaactgggtc cggtgttggc ggcgattctg tttgccagca
     3961 gcgctggcgg tgcgatgacc agcgaaatcg gtttgatgaa aacgaccgaa cagctcgaag
     4021 cgatgaacgt gatggcggta aaccctgtcg cccgcgtggt tgcgccgcgc ttttgggcgg
     4081 gcgtgttttc catgccgctt ttggcttcga ttttcaacgt ggcgggtatt ttcggcgcgt
     4141 atttggtcgg tgtaagctgg ctgagcttgg acagcggcat tttctggtcg caaatgcaga
     4201 acaacatcac gatacattac gatgtaatca acggtctgat caaatccgcc gcgttcggcg
     4261 tggcggtaac gctgattgcc gtgcatcagg gcttccactg cgtcccgacc tcggaaggca
     4321 ttttgcgcgc cagcacgcgc acggtggttt cgtccgccct gacgattttg gcggtcgatt
     4381 ttatattgac cgcgtggatg tttacagatt gacagggcgt ctgaagacga aacgcggaac
     4441 atcggatatt caaggaactt taatgaaaaa gaacatattg gaattttggg tcggactgtt
     4501 cgtcctgatt ggcgcggcgg cggttgcctt tctcgctttc cgcgtggcgg gcggcgcggc
     4561 gttcggcaat tccggcaaga cttataccgt atatgccgat ttcggcgaca tcggcggttt
     4621 gaaggtcaat gcccccgtca aatccgcagg cgtattggtc gggcgcgtcg gcgctatcgg
     4681 acttgacccg aaatcctatc aggcgagggt gcgcctcgat ttggacggca agtatcagtt
     4741 cagcagcgac gtttccgcgc aaatcctgac ttcgggactt ttgggcgagc agtacatcgg
     4801 gctgcagcag ggcggcgaca cggaaaacct tgctgccggc gacaccatct ccgtaaccag
     4861 ttctgcaatg gttctggaaa accttatcgg caaattcatg acgagttttg ccgaaaaaaa
     4921 tgccgacggc ggcaatgcgg aaaaagccgc cgaataaacg tatgccatca tattcaaact
     4981 aggacactta atcatgaaaa aatcctccct catcagcgca ttgggcatcg gtattttgag
     5041 catcggcatg gcatttgccg cccctgccga cgcggtaaac caaatccgtc aaaacgccac
     5101 tcaagtattg agcatcttaa aaagcggtga tgccaacacc gcccgccaaa aagccgaagc
     5161 ctatgcgatt ccctatttcg atttccaacg tatgaccgca ttggcggtcg gcaacccttg
     5221 gcgcaccgcg tccgacgcgc aaaaacaagc gttgaccaaa gaatttcaaa ccctgctgat
     5281 ccgcacctat tccggcacga tgctgaaatt aaaaaacgcc aacgtcaacg tcaaagacaa
     5341 tcccatcgtc aataaaggcg gcaaagaaat catcgtccgc gccgaagtcg gcgtacccgg
     5401 gcaaaaaccc gtcaacatgg acttcaccac ctaccaaagc ggtggtaaat accgtaccta
     5461 caacgtcgcc atcgaaggcg cgagcctggt taccgtgtac cgcaaccaat tcggcgaaat
     5521 tatcaaagcg aaaggcgtgg acggactgat tgccgagttg aaggctaaaa acggcagcaa
     5581 gtaatccata agccatgccg ccagactgaa gatggcatgg attttttcct accgaacact
     5641 atgcatacag aactcaacag cggaacactg cacatcagcg gcgacatcac cgtcaaaacc
     5701 ctgaccgggg aggcttttga gcgtttccag cagcaatgcc gtctgaaaga aaccattgcc
     5761 gtcgatttcg gcggtgtcaa acgtgccgat tccgcctgcg tgtcgctgct gctcgaagtg
     5821 ctgcgcggct gcaaaggcag cgtcaggctg accggcattc ccgaatccgt gcgcgcgctg
     5881 tccgaactgt acgaaatcaa agactggctg aaatcatgaa aaaaaccgcc tatgccttcc
     5941 tcctgctgat cgggttcgct tccgcccctg catttgccga aacccgcccc gccgaccctt
     6001 atgaaggcta caaccgcgcc gttttcaaat tcaacgacca agccgaccgc tacattttcg
     6061 cccctgccgc gcgcggctac cgcaaagttg cgccgaaacc cgtccgcgcc ggcgtgtcca
     6121 atttttttaa taacctgtgc gacgtggtca gcttcggcag caatatcttg cgcttggaca
     6181 tcaaacgcgc aagcgaagac cttgtccgcg tcggcatcaa caccactttc ggtttgggcg
     6241 ggcttatcga catcgccggc gcgggcggca ttcccgacaa taaaaacacc ttgggcgaca
     6301 cgtttgcttc gtggggatgg aaaaacagca attatttcgt gttgcccgtc ttagggccgt
     6361 ccaccgtccg cgacgcgctc ggcacgggta ttacctccgt ttattcgccc aagaatatcg
     6421 tcttccgcac ccctgtcgga cgctggggca cgactgccgt atccgccgtc agtacgcgcg
     6481 aaggcctgct cgatttgacc gacagcctgg acgaagccgc catcgacaaa tacagctaca
     6541 cgcgcgacct ctatatgaaa gtccgtgcgc ggcagaccgg tgcaacacct gccgaaggta
     6601 cggaagataa catcgacatc gacgaattgg tcgaaagtgc cgaaaccggc gcggcggaaa
     6661 ctgccgttca agaagattcc gtatccgaaa cacaggcaga agcagcaggg gaagccgaaa
     6721 cgcaacctgg aacacaacct taaagccatg ccgtctgaaa atccgtgttc ggacggcatt
     6781 cccttctgtc ggaacggaca gtccgaaacc acagattggg ctataatctc ttttttgcgc
     6841 ataccgcgcg acagcaaagg aaaaaccatg tacgaagtca accgcagcgt attcgtcctc
     6901 atccctttag aacctttttg gaactggctg caaaccctgc cgggcaacca cctcgacgga
     6961 ctgactcttg aagacatcca agccgacgcc aattcctacc ttgtccgccc gtgcgaaacc
     7021 gccgacgaag tatgggacga aatcgaagcc cgttttgaag acattttcgc cgccgaactt
     7081 gccgactggt gcgaagacga acgcgagtgg cctgcgctcg atgccgacat tttcaacgaa
     7141 tggttcgaca tccagctttc taccgtcatc accgacctcg aacacgaacc gcttgcccgc
     7201 gaagccttcc aacccatcaa cctgaactga tgaagctgac cgtccgcaac taccatctcg
     7261 acggctacgg ccatgtcaac aatgcgcgct acctcgaatt tttcgaagaa gcgcgctggg
     7321 cgttttttga agaacgcgga ctgctgcacg agttggcagg cctcatactg attgtcgccc
     7381 gcatcgacat ccgatacagc cgccccgccg tcgaaggcga cgtattgcag ttttcatgcc
     7441 gcctgaaaac ccccggcacg cgccgtattg tgctgaccca gacgattaca ctgccaaacg
     7501 gcaaaaccgc cgcagaagcc gacatcaccc tgatgcccgt ccacgccgcc acacaacgca
     7561 ccgtcagcct gcccgccacc cttgcccgcg cattggaagc cttgtccgaa tgaaaaaaat
     7621 actcaccgcc gccgtcgtcg cactgattgg catcctcctt gccatcgtcc tcatccccga
     7681 cagcaaaacc gcgcccgcct tctccctgtc cgacctgcac ggaaaaaccg tttccaacgc
     7741 cgacctgcaa ggcaaagtta ccctgattaa tttttggttt ccctcctgtc cgggttgtgt
     7801 gagcgaaatg cccaaaatca ttaaaacggc aaatgactat aaaaacaaaa acttccaagt
     7861 acttgccgtc gcccagccca tcgatccgat agaaagcgtc cgccaatatg tcaaagacta
     7921 cggtttgccg tttaccgtca tgtatgatgc ggacaaagct gtcggacagg cgttcggcac
     7981 acaggtttat ccgacttccg tccttatcgg caaaaaaggc gaaatcctca aaacttatgt
     8041 cggcgaaccc gatttcggca aactctacca agaaatcgat accgcgctgg cgcaatagcg
     8101 atgccgtctg aagcgcaaaa aacagaaacg atgttcctgt tttttcagat catccgcccc
     8161 gacacgttca gacggcattc aaccttccgg cagattcttt ttcaccactg ccggattgcc
     8221 tgccgccaaa gagtagggcg gaatgtcttt tgtaaccacc gcgcctgcgc ccacgaccga
     8281 accgcgtccg acggttacgc ccgccattac aatcaccctg tgccccggcc agacatcgtc
     8341 ctccaacgta atcggacgga tttccgtgta gccctcaaag cgttttttga acggtcaaac
     8401 ttgtggttat ttgaataaaa cagacattcc ggctccatca tcacattttt gccgaccacc
     8461 agcccacggc agatttcaca gtttgccccg atgcccgagc cgtcgcccaa aaccgtatcc
     8521 ggaaacacat acgccccgcg ttcgatattg accccgcgtc cgatatgcgg agaaacccgc
     8581 cgcgccaaaa aaccgcgtac ccgtctgccg acgatgccgc gcatatgcga cggcggcaac
     8641 accgaaccgg caaaacgcag cagcaggaaa aagaattttg atcccatcaa acctccctca
     8701 aaagaacttt ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagc cgcagacagt
     8761 acagatagta cggcaaggcg aggcaacgct gtactggttt ttgttaatcc gctatacatt
     8821 ttatcgaata tcctcttgac gtaacaacag aaaatagaga gaatgtcgga cttgtctgta
     8881 gcataggtac atcccctatg cccgcttttt tgaaaggaaa tcaaatgaaa caaggtattc
     8941 acccgaacta ccacgaagtc aacgttacct gctcttgcgg caacaaattc gcaaccaaat
     9001 ccgcgatgga aaaagaaaac ttcaatatcg aggtttgctc cctgtgccac ccgttctata
     9061 ccggcaccca aaaaatcgtc gataccaccg gccgcgtgga caaattcaac aacaaattcg
     9121 gcaacctgtt caaacgctga ttgccgcttt gcaagaatta agccctgcca tcatttcgat
     9181 ggcagggctt tttaatattt ggcacactga cttcttatct atatagtgga ttaacaaaaa
     9241 tcaggacaag gcgacgaagc cgcagacagt acagatagta cggaaccgat tcacttggtg
     9301 cttcagcacc ttagagaatc gttctctttg agctaagacg aggcaacgcc gtactggttt
     9361 ttgttaatcc actatatttt gacggggttg aagttgacat ctacaaggtt taatgtttga
     9421 atacattaaa aaaatatatt tgaatgagat gttttatgat tcaaaatgtc gttacttcaa
     9481 taatcctgta ttttgggaca gccgtagact tacttattat cctaatgtta ttttttgcca
     9541 aaagaaaaag cagaaaagac atcattaaca tctatttagg ccaatttcta ggctctgtta
     9601 gcctgatatt gctaagtttg ctttttgcat ttgtcttaga ttatattcct agtaaagaga
     9661 ttttaggttt gctcggcttg attccgattt tcctaggcat caaagttttg cttttaggag
     9721 attccgatgg agaggctatt gccaaagagg gtttgcgcaa agataataga aacctgattt
     9781 ttctagtcgc tatgattact tttgcaagtt gtggcgctga caatattggt gtctttgtcc
     9841 catattttac taccttaaat tcagcgaatt tgatagtggc tttacttacc tttctagtca
     9901 tgatttatct cttggttttt tctgcccaaa aattggcaca agtcccttct gtcggagaaa
     9961 ctttggaaaa atatagcaga tggtttgttg ccgttgttta tttaggattg gggatatata
    10021 tcctgattgg aaacaacagt tttgatatgc tatggactgt gtcgggccag gaaaaaatat
    10081 tatgaaaaag atagtatctg caaagactgc cccgtgcagt cttttttgtt gccggtcttt
    10141 ttgtgtctga tgccgtctga agcagtctct gcacgacctt tgtgcgaata tttgctacac
    10201 ttggcaatcc gtccatttct tctcacgctt atgctgacct ataccccgtc cgatgcccga
    10261 acacctgcca aaacccacga aaagccgtgg ctgctgatgc tgatggcgtt tgcctggttg
    10321 tggcccggcg tgttttccca cgatttgtgg aatcctgccg aacctgccgt ctataccgcc
    10381 gtcgaagcac tggcaggcag ccccacccct ttggttgccc atctgttcgg tcaaaccgat
    10441 ttcggcatac cgcccgtgta tctttgggtt gccgccgcgt tcaaacattt gctgtcgccg
    10501 tgggcagccg acccgtatga tgccgcacgc tttgccggcg tgtttttcgc cgttgtcgga
    10561 ctgacttcct gcggctttgc cggtttcaac tttttgggca gacaccacgg gcgcagcgtc
    10621 gtcctgattc tcatcggctg tatcgggctg attccagttg cccatttcct caaccccact
    10681 gccgccgcct ttgccgccgc cggactggtg ctgcacggtt attctttggc tcgccggcgc
    10741 gtgattgccg cctcttttct gctcggtacg ggctggacgc tgatgtcgtt ggcagcagct
    10801 tatccggcag catttgccct gatgctgccc ttgcccgtac tgatgttttt ccgtccgtgg
    10861 caaagcaggc gtttgatgtt gacggcagtc gcctcacttg cctttgccct gccgcttatg
    10921 accgtttacc cgctgctctt ggcaaaaacg cagcccgcgc tgttcgcgca atggctcgac
    10981 gatcacgttt tcggtacgtt cggcggcgtg cggcacgttc agacggcatt cagtttgttt
    11041 tactatctga aaaacctgct ttggtttgca ttgcccgcgc tgccgctggc ggtttggacg
    11101 gtatgccgca cgcgcctgtt ttcgaccgac tgggggattt tgggcgtcgt ctggatgctt
    11161 gccgttttgg tgctgcttgc cgtcaatccg cagcgttttc aggataacct cgtctggctg
    11221 cttccgccgc ttgccctgtt cggcgcggcg caactggaca gcctgaggcg cggcgcggcg
    11281 gcgtttgtca actggttcgg cattatggcg ttcggactgt ttgccgtgtt cctgtggacg
    11341 ggctttttcg ccatgaatta cggctggccc gccaagcttg ccgaacgcgc cgcctatttc
    11401 agcccgtatt acgttcccga catcgatccc attccgatgg cggttgccgt actgttcaca
    11461 cccttgtggc tgtgggcgat tacccggaaa aacatacgcg gcaggcaggc ggttaccaac
    11521 tgggcggcag gcgttaccct gacctgggct ttgctgatga cgctgttcct gccgtggctg
    11581 gacgcggcga aaagccatgc gccggtcgtc cggagtatgg aggcatcgct ttccccggaa
    11641 ttgaaacggg agctttcaga cggcatcgag tgtatcgaca taggcggcgg cgacctgcac
    11701 acgcggattg tttggacgca gtacggcaca ttgccgcacc gcgtcggcga tgtacaatgc
    11761 cgctaccgta tcgtccgctt gccccaaaat gcggatgcgt cgcaaggctg gcagacggtc
    11821 tggcagggtg cgcgcccgcg caacaaagac agtaagttcg cactgatacg gaaaatcggg
    11881 gaaaatatat taaaaacaac agattgagcc gaatttctgg attaagtgcc ggaaatcgcg
    11941 tataattgcg cgattaaacc tttataaatc agccgttttg caggcatcac acaggagcga
    12001 caattattat gatgaccctc tattccggca ttacctgccc cttcagccac cgctgccgct
    12061 tcgttttgta cgaaaaaggt atggattttg aaatcaaaga cgtcgatatt tacaacaaac
    12121 ccgaagacct cgccgtcatg aatccgtata accaagttcc cgtgctggtc gagcgcgatt
    12181 tggtgctgca cgagtccaac atcatcaacg aatacatcga cgaacgcttc cctcatccgc
    12241 agcttatgcc cggcgatccc gttatgcgcg gccggggccg actggtgctg taccgtatgg
    12301 aaaaagaatt gttcaaccac gtccaagtgt tagaaaaccc cgccgccacc aacaaggaac
    12361 aggcaaaagc gcgcgaagcc atcggcaacg gtctgaccat gcttgcccct tcgttcagca
    12421 aaagcaaata catcctcggc gaagattttt ctatgattga tgtcgccctt gctccgctgc
    12481 tgtggcggct cggccactac gatgtcaaac tgggcaaaag tgccgcgccg ctgctcaaat
    12541 acgccgagcg catcttccag cgcgaagcct ttatcgacgc gctgacacct gcggaaaagg
    12601 cgatgcgcaa ataagtttga aatgcttgca aaacccaccg ttttgcaggc attttcctat
    12661 tttggcgtac aacacggaac ccattatgcc cacttccacc aaaccctaca tcctccgcgc
    12721 actttgcgaa tggtgcagtg ataacagcct cacgccgcac attcttgtct gggtcaacga
    12781 acacacgcgt gtccccatgc agtacgtccg cgacaacgaa attatgctca acatcggcgc
    12841 gaccgccacg caaaaccttc aaatcgacaa cgattggatc agcttttccg cccgcttcgg
    12901 cggacaggcg cacgacatat ggatacctgt cggacacgtc ctcagccttt tcgcacggga
    12961 gaccggcgaa ggtatggggt ttgaattgga agcgtatcgc cccgacacgc cgcctgaaaa
    13021 cacctctgcc gaaaccgcgc cccggcccgc caaaaaaggc ttgaaattgg tcaaataaat
    13081 ctatgccgtc tgaacggaat cgtgtttcag acggcatttt gtccgatggg gcgcaaacgg
    13141 aatccgttta tcggcaaaac ccgtttcggt gtatcgaaac cgtgttgccc ctgccgattc
    13201 gatattggga cattgaaaat gccgtctgaa cctgcgatac gggcttcaga cggcattttg
    13261 tccgatattc gggcaatcag gcggtcagca cggctttcag gattttgttg acttcgccca
    13321 tatcggcttt gccggcaagt cgggttttca atacgcccat cactttgccc atatccgcca
    13381 tacctgccgc tccggtttcg gcaacggcgg cttcgacctc ggtacggatt tcgccggcag
    13441 agagcatttg gggaaggtag cggtgcagta cctcgatttc ggcgttttct ttgtctgcca
    13501 aatcctggcg gccggcttca gtgtagattt tcgcgctgtc tttgcgctgt ttgaccattt
    13561 tggtcaggat ggcggtgatt ttggcatcgt cggcttcggt gcgttcgtcc acttcaaact
    13621 gtttgacggc ggcgttgatg aggcggatgg tgccgaggga aacttggtct ttggcgcgca
    13681 tcgcggtttt catgtcttcg gtaaggcgga ttttcaggct cataatgtcc tcgctgggat
    13741 gtcggatgga aacggcgggt ttcgatgccg tctgaaaagc aaaacacacc gcagagcagg
    13801 tcttgcggtg tgtcctcata tcacagggct gacctgtaat ctgtacttga acgtttagta
    13861 cattttgggc ggcagttgtt ggctgcgcag gcgtttttgc aggcgtttta cggctgccgc
    13921 ttttttgcgt ttgcgttcgg tagtcggttt ttcgtaggct tcgcgggcgc gcagctcggt
    13981 cagcaggccg gttttttcta cggcgcgttt gaaacggcgc atagcgactt caaatggttc
    14041 attctctttt acgcggattg caggcatttt atttccttta ataaattcgg ttgtttcatc
    14101 tgcccatcat atcggtggaa agggtagggc agacggtgtt gaaggtttac cgtatcgctg
    14161 tgccttgcgg cggcgggata cgcgccgtga cggaaacatc acctcctaac gggtcggctt
    14221 gtgccgtgtc aagctgcttg aaaaagcagg aaaaacaatt ttcggattgt cttatattta
    14281 tggttcgccg tcaatgccgt tcgggataaa aatgccgtct gaaagaccgg gcgggtttca
    14341 gacggcatcg gtacgtcagc gtgcaggaac aatgcccata cgctgtttga gcgggatgtg
    14401 cggcgcgccg aagagggcgg cgtagtaggc ggcattggtc atcacttttt tgacatagtc
    14461 gcgcgtttcg gaaaacggga tggtttcggc atataccgcg ccttcgaggg gcgtgtccgc
    14521 ctgccatcgg cgcgccctgc cgggaccggc gttatagcct gcggtggcga ggacttcgtt
    14581 gttttgcagg cggcgtttgg tgtccgccat ataccacgtc cccatacgga tattgccgtc
    14641 ggcggtgtaa agttgtgcgg catccatacc gattttgccg gcgatttcgc gcgcggtggc
    14701 aggcataacc tgcatcagcc cctgcgcgcc tacgcgggat tgcgcgccta taacgaagcg
    14761 gctttcctga cgaatcaggc cgtaaaccca agccggatcg acattaacat tttgcgcgtg
    14821 gcggattacc gtgtctttaa acggcgaaat atagcgcaag gtgtagttga gtttgcggtc
    14881 ggtgcgttcc gcgctgttga ccgccatatc gtaaaaaccg tggtcgaacg cggtttgtgc
    14941 ggcggtcagc agcttgtctt cgtcgaaccc gcgtgtggca aaacgccatt ccgcctgagc
    15001 ctgacggcgc attttcgcat cgccggcggt ccggctgttt tggaacagta ccagcgcacg
    15061 cttaatcgcg ccgtcttccg ccatacggag gacgctgttt gcgtcggcat cgggaacatt
    15121 gttgcgcgta tcgattttgc gccccaactc ttcccctgcc agcaccgcat aaaaattcct
    15181 gcccgtcgct gccgcctgtt tgtaaagttt ttccgcctct tgcgtgttgc ccgttgcggc
    15241 gcggctgcgt gccagccagt agagccaggt cgggcttttt tgcagttttt cgggcatatg
    15301 cgagataacg gaggccagct cgtcccaacg tcgggcgcgc aaggcggcgc gggcgtacca
    15361 ctcgatttgg tcgtcggtca gttggcggcg gtcggcaacc ttgccgtaat agtccaaggc
    15421 ggcaggcaca ttgaggtttt gcgactgata atgccccaat acgccccacg cgaaactgcg
    15481 ttgttcgagg cttaaaccgc tttccatttc ggacagcagg gcggcggcat tcggcgattt
    15541 gcgtgcttct ttgccgatga cgttcaacag agcatattcg cgcgaacctt gtgtaccgcc
    15601 gtcaaacggg ctgcccaatg cggcggcaag gttgcgtgcg tctgtggttt ggcggccggc
    15661 cagcagtccg cgcacgcgcc tccaggcgtc gttgccgtcc aacaagccgg atgcggctgc
    15721 ctgttccaac agtttggtgc agcccgaagg cagtttgccc gtatttttga ccagttcggc
    15781 ggcacgcgta tagtcgttgc ggctcgaatc ggcgtagcat tcgacttctt gggcgcgccc
    15841 tgccggttcg agtttggcat attcctgtgc aaacagcgtc cactgtttgc gtgcgcccaa
    15901 agacttcagc cactcgttgc ggacattttc cgccatcgca ctgtcgccgg cgttttccaa
    15961 ataggcggcg acggcggcat cgtttttctg tttcactgca tccagtgcgg acgggtagcc
    16021 gccgtaatct gccagcgttt ttccttccgg ttcggcaggg cgggtgggaa cgcttgccga
    16081 aaggtcggca gtttctatat tgtctgccgg ggtcttgccg gctggcagtg tgtttgtcga
    16141 agaacacgcg gcaagcacca gagccgccag cagcggcagg gaatgcttca tagagggtag
    16201 gtacatcgga tttccttaag aatcggaacc ctgaacggtc agggttggaa aagacaaaat
    16261 gccgtctgaa caggcgtttg catgaattat atgccgaaac tgcaccggct ttggaatgtt
    16321 tccgacataa tttatatttt tcaatcattt gccgttttgg tgcgaacctc tgcctttgcc
    16381 cgtttcagac ggcattgtcc gaaatggttg cctgcttcct gccttattga caaaaaaacg
    16441 ctttcccgat aatatcctac gaaaattaac ctgccgattt gacacagctt gcgggcataa
    16501 cagctaaagc gttccgacaa tttcagcttt atcttccgcg cccgttgtgt ccgacatcgg
    16561 gctttgttgt atgggaaaga caatgattat tttggacaag gtttccaagc attaccaaac
    16621 gcgcgacaag acccgttttg ccgccgtcga gccgaccagc ctcgaaatcc gcgacggcga
    16681 aatcttcggg ctgatgggtt attcgggtgc aggcaaatcc accctgttgc gcctgattaa
    16741 cctgttggaa cgccccgaca gcggcaaggt caacgtctgc ggacaagagc tgaccgcgct
    16801 cgatgccgcc gcattgcgtc aggctcggca gaatatcggc atggtgtttc agcagttcaa
    16861 cctgttgaac aaccgcaccg tcgccggcaa cgtcgccttc ccattggaaa tcgccggatg
    16921 gccgtctgaa aaaatcaaag cgcgcgttgc cgaatgcctc gaaatcgtcg gattgaccga
    16981 acgcgccggc cactatcccg cccagctttc cggcggacaa aaacagcgcg tcggcatcgc
    17041 ccgcgcgctc gcatccaagc cgcaagtcat cctcgccgac gaacccactt ccgccctcga
    17101 ccccgccacc acgcgcagcg tcttggaatg tttggaagac atcaacaaac gcttcaacgt
    17161 aaccatcgtc atcgtaaccc acgaaatgag cgtcatcaga cgactgtgcg accgcgccgc
    17221 cctgttggat aaaggcaaag tcgtcgaaat cgtcgaagta cgcggcaacc aaatccacgc
    17281 ccgatccgac atcgggcgcg aactcatcag agaggactga aatggcagac ctgaccttcc
    17341 aacaagccgt ttccaacatc atcggtatga aaggcgaaat cgtccgcgca ttgggcgaaa
    17401 ccttcgtaat ggtcggcttg tccaccacat tcgccgtcgt tttcggcacg ttgctgggcg
    17461 tgctgctctt cgtaacctcc agccgccagt tgcattacaa caagccggta aatttcctgc
    17521 tcgacaacct cgtcaacctc atgcgcgcct tccccttcgt cattttgatg attgcgatga
    17581 tacccgccac acgcgccatc gtcggcagca ccatcggccc gattgccgcc tcgctggtgt
    17641 tgagcgtgtc gggattgttt tattttgccc gactggtgga acaaaacctg cgcgaagtcc
    17701 ccaaaggcgt gattgaagcc gccgccgcga tgggtgcgcc tccgattgcc atcgtctgca
    17761 aagtcctctt gaacgaagcg cgcgcgggca tggtttccag cattaccgtg cttgccatcg
    17821 ggcttttgtc atacagcgcg gcggcaggga tgataggcgg cggcggcttg ggcgacctcg
    17881 ccatccgcta cggctactac cgctaccaaa ccgaagtcat cgtcttcatc gtcgccctcc
    17941 tcgtgctgct ggtcatcctg attcaaagca ccggcaacgc gttggcgcgg aaactcgaca
    18001 aacgttaaac ccaaaatgcc gtctgaagcc cggatgaagc cggccttcag gcttgagaac
    18061 gatacggtag ggtgaaatat ccgccgcacc gttaaaaatg ctataaaatc cccctgttcg
    18121 cggcaaatgc cgtctgaacg ccgaatccgg acggcagggt ttcctgcccg tcatttttgt
    18181 ttgaaaccgc cacaacatca ggagaaaata tgaaaacctt cttcaaaacc ctttccgccg
    18241 ccgcactcgc gctcatcctc gccgcctgcg gcggtcaaaa agacagcgcg cccgccgcat
    18301 ccgcttctgc cgccgccgac aacggcgcgg cgaaaaaaga aatcgtcttc ggcacgaccg
    18361 tcggcgactt cggcgatatg gtcaaagaac aaatccaacc cgagctggag aaaaaaggct
    18421 acaccgtcaa actggtcgag tttaccgact atgtgcgccc gaatctggca ttggctgagg
    18481 gcgagttgga catcaacgtc ttccaacaca aaccctatct tgacgacttc aaaaaagaac
    18541 acaatctgga catcaccgaa gtcttccaag tgccgaccgc gcctttggga ctgtacccgg
    18601 gcaagctgaa atcgctggaa gaagtcaaag acggcagcac cgtatccgcg cccaacgacc
    18661 cgtccaactt cgcccgcgtc ttggtgatgc tcgacgaact gggttggatc aaactcaaag
    18721 acggcatcaa tccgctgacc gcatccaaag cggacatcgc cgagaacctg aaaaacatca
    18781 aaatcgtcga gcttgaagcc gcgcaactgc cgcgcagccg tgccgacgtg gattttgccg
    18841 tcgtcaacgg caactacgcc ataagcagcg gcatgaagct gaccgaagcc ctgttccaag
    18901 aaccgagctt tgcctatgtc aactggtctg ccgtcaaaac cgccgacaaa gacagccaat
    18961 ggcttaaaga cgtaaccgag gcctataact ccgacgcgtt caaagcctac gcgcacaaac
    19021 gcttcgaggg ctacaaatcc cctgccgcat ggaatgaagg cgcagccaaa taaggcagtc
    19081 gtataaaatg atgccgtctg aaccgtatcc gtgttcagac ggcatttttg tcctttaatc
    19141 cgccattccc tgccattccg ccgaatccgg cgtatcgatt ccgaacagcg acaaagcgtg
    19201 tgcaacactg tgcgccacta tgtcgtccgc cgtctgcggt ttgcggtaca tcgcaggaac
    19261 agggggaaac accacgccgc ccatttccgt tacccgcttc atattgtcca aatgggcaag
    19321 gttcagcggc gtttcgcgca ccatcagcac cagccgccgc ctttccttca aaaccacatc
    19381 cgccgcacgc gtcaagaggt tgtcgccgaa gccgtgcacg accgaggcaa gcgtccgcat
    19441 cgaacagggg gcgaccagca tcccgtccgt tttaaacgta ccgctggcaa tgcacgcccc
    19501 gatattgccg atcggatgca cgaagtccgc caaggcatat acctcgtctc tcgcataagc
    19561 cgtttccgaa gcgcgcgcca tctccgcacc tttcgatacc acaaggtgcg tttcgatatc
    19621 ttgcgcgcgt aaaagttcca aagccttcac gccgtattgg aaaccgctcg ccccactgat
    19681 gccgattatc aaacgccgta ccatcatccg cctttcccat aaaaccgcct gcaacggcaa
    19741 accggctatt atagtgaaaa aacagaaatc cgataaatac ggatacaaat tgtcggcaac
    19801 acccaatatc cgataaaata tccgatttaa catcctatct gaataggcac gggagggcgg
    19861 tatggcaaaa gtaaaaggcg gattggggcg cggtttggat tcgctgctcg ccaacggcgc
    19921 agacaacagc agcggcgacc gattgaccac ggttgcggtt aaagatatcc ggcccggccg
    19981 ctatcaggcg cgtgttcaaa tcgatgacga agccttgcag gagctggcag attcgattaa
    20041 ggcgcaaggc gtgatacagc ccgtcatcgt gcgcgaacac ggactgtccc gatacgaact
    20101 gattgcgggg gagcggcgtt ggcgcgccgc acagattgcc ggtctgaccg aaatccctgc
    20161 cgttatcaaa accatcagcg acgaaaccgc attggcaatg ggtttgatcg aaaacctcca
    20221 gcgcgaaaac ctcaacccca tcgaagaagc acaaggcttg aaacgccttg ccgacgagtt
    20281 cggactgacc cacgaaacca tcgcccaagc cgtcggtaaa agccgaagcg cgatttccaa
    20341 cagcctgcgc cttttaagcc tgcccgaacc cgtgcaggaa atgctttacc aacgccgcct
    20401 cgaaatgggg cacgcccgcg cactgctgac cctgcccgtc gtcgaacagc tcgaattggc
    20461 gcaaaaggcc gtcaaaaacg gctggtcggt gcgcgaagtc gaacgccgca gccaagccgc
    20521 ccttcaaaac aaacgtcccg agcccaaaaa gactgccgcc gccgacatcg gccgcctgaa
    20581 tgatttgctg actgaaaaac tgggtgtcaa cgccgaaatc aaaaccgcca accataaaaa
    20641 aggcaaaatc atcctgcatt tcgacacgcc cgaaaccttc gaccatatct tgaagcagtt
    20701 gggcatagat taccgacctt aattttgcgg gatataccgt ctgaaatata gagaatagct
    20761 ttccagattt taagtgggaa atataattct attgacattt ttctgcttca cgtaagaatc
    20821 gttttcctgt tttcattttt aattttcgag gaaattatga acacacgcat catcgtttcg
    20881 gctgcgttcg ttgcgttggc attagcaggt tgcggctcaa tcaataatgt aaccgtttcc
    20941 gaccagaaac ttcaggaacg tgccgcgttt gccttgggcg tcagcccaaa tgccgtaaaa
    21001 atcagcaacc gcagcaatga aggcatacgc atcaacttta ccgcaactgt gggtaagcgc
    21061 gtgagccaat gctatgttac cagtgtaatc agcacaatcg gcgttaccac ttccgatgca
    21121 atttgtttgg gaggcggaac gcacaaaggc aaaagtcaat gcaatgcttt gcttaaagcg
    21181 gcaggccgtt gctaatcctt tattcggaaa aggtcggcgg attcgcattt gaagtgcaac
    21241 tttccctaac agaaaaaggc cagtatgcgg tagcatacga cctttc
//
LOCUS       DAWFHY010000031        21248 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.031, whole genome shotgun sequence.
ACCESSION   DAWFHY010000031 DAWFHY010000000
VERSION     DAWFHY010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 21248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21248
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.031"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(240..488)
                     /locus_tag="WGQ68_001003"
                     /pseudo
     CDS             complement(240..488)
                     /locus_tag="WGQ68_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(517..846)
                     /locus_tag="WGQ68_001004"
     CDS             complement(517..846)
                     /locus_tag="WGQ68_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0714825.1"
                     /translation="MGSLIIEDLQEGFGKEAVKGKEITVHYTGWLENGTKFDSSLDRR
                     QPLTITLGVGQVIKGWDEGFGGMKEGGKRKLTIPSEMGYGARGAGGVIPPHATLIFEV
                     ELLKVYE"
     gene            complement(924..1142)
                     /locus_tag="WGQ68_001005"
     CDS             complement(924..1142)
                     /locus_tag="WGQ68_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2061 domain-containing protein"
                     /protein_id="HEZ0714826.1"
                     /translation="MLKTLTFAALHFSVAFSVTYVLTGSIGVSGAVALVEPLINTVVF
                     YFHEKAWNLYEKNKTVKQTQPFQLHRCS"
     gene            1264..2217
                     /locus_tag="WGQ68_001006"
     CDS             1264..2217
                     /locus_tag="WGQ68_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEZ0714827.1"
                     /translation="MNHKKIVVLDADTLPGRVFHFDFPHELAVYGTTGADETAERVRD
                     AHIVITNKVMISADIIAANPQLELIAVSATGVNNVDIGAAKAAGVAVCNVRAYGNESV
                     AEHAFMLMIALMRNLPAYQRDVAAGLWEKSPFFCHYGAPIRDLNGKTLAVFGRGNIGR
                     TLAGYAQAFGMGVVFAEHKHAPDVREGYVSFEDAVRAADVLSLHCPLNAQTENMIGEN
                     ELRQMKPGAVLINCGRGGLVDENALLAALKYGQIGGAGVDVLTEEPPRGGNPLLNARL
                     PNLIVTPHTAWASREALDRLFDILLANIHAFVKGEAQNRVV"
     gene            complement(2292..4349)
                     /gene="metG"
                     /locus_tag="WGQ68_001007"
     CDS             complement(2292..4349)
                     /gene="metG"
                     /locus_tag="WGQ68_001007"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEZ0714828.1"
                     /translation="MTRKILVTSALPYANGSIHLGHMVEHIQTDVWVRFQKLRGHECH
                     YCCADDTHGTPVMLAAQKQGIAPEDMIAKVREEHLADFTDFGIGYDNYYSTHSPENKQ
                     FSQDIYRALKANGKIESRVIEQLFDPEKQMFLPDRFVKGECPKCHAQDQYGDNCEVCG
                     TTYSPTELINPYSAVSGAKPELRESEHFFFKLGECADFLKAWTSGNNPHDGKPHLQPE
                     ALNKMKEWLGEGEETTLSDWDISRDAPYFGFEIPDAPGKYFYVWLDAPVGYMASFKNL
                     CDRIGVDFDEYFKADSQTEMYHFIGKDILYFHALFWPAMLHFSGHRAPTGVYAHGFLT
                     VDGQKMSKSRGTFITAKSYLEQGLNPEWMRYYIAAKLNSKIEDIDLNLQDFISRVNSD
                     LVGKYVNIAARASGFIAKRFEGRLKDVADSALLAKLAAESDTIAEQYESREYAKALRD
                     IMALADIVNEYVDANKPWELAKQEGQDERLHEVCSELINAFTMLTAYLAPVLPQTAAN
                     AAKFLNLEAITWANTRETLGKHAINKYEHLMQRVEQKQVDDLIEANKQSIAAAAVPAA
                     EESKYEKVAEQASFDDFMKIDMRVAKVLNCEAVEGSTKLLKFDLDFGFEKRIIFSGIA
                     ASYPNPAELNGRMVIAVANFAPRKMAKFGVSEGMILSAATADGKLKLLDVDAGAQPGD
                     KVG"
     gene            complement(4465..6303)
                     /gene="glmS"
                     /locus_tag="WGQ68_001008"
     CDS             complement(4465..6303)
                     /gene="glmS"
                     /locus_tag="WGQ68_001008"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEZ0714829.1"
                     /translation="MCGIVGAIRAHHNVVDFLTDGLKRLEYRGYDSSGIAVNTDGKIK
                     RVRRVGRVQLMEDAAREKGISGGIGIGHTRWATHGGVTEPNAHPHISGGMIAVVHNGI
                     IENFESERKRLEGLGYRFESQTDTEVIAHSINHEYAQNGGRLFEAVQEAVKRFHGAYA
                     IAVIAQDKPDELVVARMGCPLLVALGDDETFIASDVSAVIAFTRRVAYLEDGDIALLA
                     SDGIKRLTDKSGLPAERKVKVSELSLASLELGPYSHFMQKEIHEQPRAIADTAEVFLD
                     GGFIPENFGKNAKSVFESIRSVKILACGTSYYAALTAKYWLESIAKIPADVEIASEYR
                     YRSVIADPDQLVITISQSGETLDTMEALKYAKSLGHRHSLSICNVMESALPRESSLVL
                     YTRAGAEIGVASTKAFTTQLVALFGLAVTLAKVRGLVSEEDEARYTEELRQLPGSVQH
                     ALNLEPQIAAWAQQFAKKTSALFLGRGIHYPIALEGALKLKEITYIHAEAYPAGELKH
                     GPLALVDENMPVVVIAPNDSLLDKVKANMQEVGARGGELFVFADLDSNFNATEGVHVI
                     RAPRHVGELSPVVHTIPVQLLSYHVALARGTDVDKPRNLAKSVTVE"
     gene            complement(6503..6610)
                     /locus_tag="WGQ68_001009"
                     /pseudo
     CDS             complement(6503..6610)
                     /locus_tag="WGQ68_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6690..7208
                     /locus_tag="WGQ68_001010"
     CDS             6690..7208
                     /locus_tag="WGQ68_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714830.1"
                     /translation="MKQMLLAVGVAAVLAGCGKDAGGYEGYWREKSDKKEGMIAVKKE
                     KGNYFLNKINVVTGKEESMLLSEKDGALSINAGIGEIPIKLSDDGKELYVERRQYVKI
                     DAAMKDKIIAHQKKCGQTAQAYLDARNALPSNQTYQQRQAAIEQLKRRFEAEFDELEK
                     EIKCNGKPTLLF"
     gene            complement(7625..8950)
                     /locus_tag="WGQ68_001011"
     CDS             complement(7625..8950)
                     /locus_tag="WGQ68_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HEZ0714831.1"
                     /translation="MKKYLFRAALYGIAAAILAACQSKSIQTFPQPDTSVINGPDRPV
                     GIPDPAGTTVGGGGAVYTVVPHLSLPHWAAQDFAKSLQSFRLGCANLKNRQGWQDVCA
                     QAFQTPVHSFQAKQFFERYFTPWQIAGNGSLAGTVTGYYEPVLKGDDRRTAQARFPIY
                     GIPDDFISVPLPAGLRSGKALVRIRQTGKNSGTIDNAGGTHTADLSRFPITARTTAIK
                     GRFEGSRFLPYHTRNQINGGALDGKAPILGYAEDPVELFFMHIQGSGRLKTPSGKYIR
                     IGYADKNEHPYVSIGKYMADKGYLKLGQTSMQGIKSYMRQNPQRLAEVLGQNPSYIFF
                     RELTGSRNDGPVGALGTPLMGEYAGAVDRHYITLGAPLFVATAHPVTRKALNRLIMAQ
                     DTGSAIKGAVRVDYFWGYGDEAGELAGKQKTTGYVWQLLPNGMKPEYRP"
     gene            9161..10000
                     /locus_tag="WGQ68_001012"
     CDS             9161..10000
                     /locus_tag="WGQ68_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HEZ0714832.1"
                     /translation="MLVAFLIMLREGIEAALIVGIVAGFLKQSGHSKLMPKVWFGVVL
                     ASLMCLGLGYGIHSATGEIPQKQQEFVVGIIGLVAVAMLTYMILWMKKAARSMKQQLQ
                     DSVQAALNRGSGQGWALVGMAFLAVAREGLESVFFLLAVFKQSPTWQMPAGAVAGGLA
                     AAVIGALIYQGGMRLNLAKFFRWTGAFLIIVAAGLLAGSLRALHEAGIWNALQDIVFD
                     SSKYLHEDSPLGVLLGGFFGYTDHPTQGEALVWLLYLIPVITWFLCGSRPSETLTRKE
                     ELK"
     gene            9997..11163
                     /locus_tag="WGQ68_001013"
     CDS             9997..11163
                     /locus_tag="WGQ68_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HEZ0714833.1"
                     /translation="MRKFNLTALSVMLALGLTACQPPEAEKAAPAASSEAQTANEGGS
                     VSIAVNDNACEPMELTVPSGQVVFNIKNNSGRKLEWEILKGVMVVDERENIAPGLSDK
                     MTVTLLPGEYEMTCGLLTNPRGKLVVTDSGFKDTANEADLEKLSQPLADYKAYVQGEV
                     KELVAKTKTFTEAVKAGDIEKAKSLFADTRVHYERIEPIAELFSELDPVIDAREDDFK
                     DGAKDAGFTGFHRIEYALWVEKDVSGVKEIAAKLMTDVEALQKEIDALAFPPGKVVGG
                     ASELIEEVAGSKISGEEDRYSHTDLSDFQANVDGSKKIVDLFRPLIETKNKALLEKTD
                     TNFKQVNEILAKYRTKDGFETYDKLGEADRKALQASINALAEDLAQLRGILGLK"
     gene            11306..12571
                     /gene="efeB"
                     /locus_tag="WGQ68_001014"
     CDS             11306..12571
                     /gene="efeB"
                     /locus_tag="WGQ68_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HEZ0714834.1"
                     /translation="MSTNNPTQPTRRTLFKTAIAAGAVGAIGGYLGGKKQGETAERTA
                     ESQHSPQAYPCYGEHQAGIVTPQQAFSIMCAFDVTAQSAKQLENLFRTLTARIEFLTQ
                     GGEYQDGDDKLPPAGSGILGKAFNPDGLTVTVGVGSSLFDGRFGLKDKKPVHLQEMRD
                     FSNDKLQKSWCDGDLSLQICAFTPETCQAALRDIIKHTVQTAVIRWSIDGWQPKSEPG
                     AMAARNLLGFRDGTGNPKVSDPKTADEVLWTGVAANSLDEPEWAKNGSYQAVRLIRHF
                     VEFWDRTPLQEQTDIFGRRKYSGAPMDGKKEADQPDFAKDPERNTTPKDSHIRLANPR
                     DPEFLKKHRLFRRAYNYSRGLASSGQLDVGLVFVCYQANLADGFIFVQNLLNGEPLEE
                     YISPFGGGYFFVLPGVEKGGFLGQGLLGV"
     gene            complement(12643..12972)
                     /locus_tag="WGQ68_001015"
     CDS             complement(12643..12972)
                     /locus_tag="WGQ68_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEZ0714835.1"
                     /translation="MSLPPCPQCASEYTYEDGGQYICPECAHEWNETESAADLAAQVL
                     DANGAVLQNGDTVILIKDLKVKGSSMVIKQGTKVKGIRLQEGDHNIGCKIDGSAMNLK
                     SEFVKKA"
     gene            complement(13048..14418)
                     /gene="glmU"
                     /locus_tag="WGQ68_001016"
     CDS             complement(13048..14418)
                     /gene="glmU"
                     /locus_tag="WGQ68_001016"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEZ0714836.1"
                     /translation="MPQNTLNIVILAAGKGTRMYSKMPKVLHRIGGKPMVGRVIDTAA
                     ALNPQNICVVIGHGKEQVLDTVKRDVVWVEQTEQLGTGHAVKTALPHLSAEGRTLVLY
                     GDIPLIDVKTLETLLEAAGDEVGLLTDVPTAPTGLGRIIRDGNGSVTAIVEEKDANTA
                     QKAVKETNTGILVLPNAKLENWLNSLSSNNAQGEYYLTDLIAKAVADGIKVHPVQVRA
                     SYLAAGVNNKLQLAELERIFQTEQAQELLKAGVTLRDPARFDLRGRLKHGQDVVIDAN
                     CIFEGEIELGDNVEIGANCVIKNAKIGANSKIAPFSHLESCEVGENNRIGPYARLRPQ
                     AKLAADVHIGNFVEVKNAAIGNGTKANHLTYIGDAEVGSKTNFGAGTIIANYDGVHKH
                     KTVIGDEVRIGSNCVLVSPVKIGNKVTTGAGSTITRNVEDNKLALARARQTVIEGWVR
                     PEKDKQ"
     gene            complement(14473..14745)
                     /locus_tag="WGQ68_001017"
     CDS             complement(14473..14745)
                     /locus_tag="WGQ68_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex protein"
                     /protein_id="HEZ0714837.1"
                     /translation="MRKTFLILMTAAALLSGCAWETYQDGNGKTAVRQKYPAGTPVYY
                     QDGSYSKNMNYNQYRPERHAVLPDQTDGSEQGVRGQNWQKPKFQNR"
     gene            complement(14738..15451)
                     /locus_tag="WGQ68_001018"
     CDS             complement(14738..15451)
                     /locus_tag="WGQ68_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEZ0714838.1"
                     /translation="MQENPTVWLFDLDNTLHNADAGIFTLINRAMTRYMARRLKLSES
                     AASDLRQDYWHRYGATLAGLQIHHPEIDIAEFLRESHPIDEVLTRLHGMPKTQNTLSR
                     LKGRKAVFSNGPSFYVRAIVGALGLENRFDALFGTDDFGLLYKPNPQAYLNVCRLLDV
                     PPECCIMVDDSADNLHQAKALGMKTVRFGAKVHDLPFIDASVSDMAQLAQYAETLSEH
                     RQNHYNTRTTPENTKECYA"
     gene            15510..16511
                     /locus_tag="WGQ68_001019"
     CDS             15510..16511
                     /locus_tag="WGQ68_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0714839.1"
                     /translation="MKRKIWLLPLLAVSAYLQAQTEVRLAVHKSFSLPKGLIARFERA
                     NDAKVSIIQAGGANEMLNKLILSRANPIADAVYGLDNANIGKAREMGILAAAQPESAP
                     VAVGLPSALAVDYGYVSINYDKKWFEGKKLPLPQTLQDLTRPEYKNLLVVPSPATSSP
                     GLGFLMANISGMGEEGAFKWWAQMRQNGVKVAKGWSEAYYTDFSHNGGAYPLVVGYAA
                     SPAAEVYFSKGKYSEPPTGNLFLKGGVFRQVEGAAVLKGAKQPKLAAKLVQWLQSREV
                     QQAVPSEMWVYPAVKNTRLPDVFRFAQAPTHTTAPAQRDIDANQRGWVSRWIRTVLK"
     gene            16605..17453
                     /locus_tag="WGQ68_001020"
     CDS             16605..17453
                     /locus_tag="WGQ68_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0714840.1"
                     /translation="MDFKQFDFLHLISVSGWEHLAEKAWAFGLNLAAALLIFLVGKWA
                     AKRIVAVMRAAMTRAQVDATLISFLCNVANIGLLILVIIAALGRLGVSTTSVTALIGG
                     AGLAVALSLKDQLSNFAAGALIILFRPFKVGDFIRVGGFEGYVREIKMVQTSLRTTDN
                     EEVVLPNSVVMGNSIVNRSTLPLCRAQVIVGVDYNCDLKVAKEAVLKAAVEHPLSVQN
                     EERQAAAYITALGDNAIEITLWAWANEADRWTLQCDLNEQVVENLRKVNINIPFPQRD
                     IHIINS"
     gene            17479..17964
                     /locus_tag="WGQ68_001021"
     CDS             17479..17964
                     /locus_tag="WGQ68_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEZ0714841.1"
                     /translation="MDALHTIARNLTKKRQTVSCAESCTGGLLAAVFTSVAGSSQWFD
                     QSFVTYSNKAKEDRLGVLPETLLEHGAVSRQTVYEMARGAKAVAQADYAVGISGIAGP
                     GGGSESKPVGTVWFGFAFPGGSCEAMRRFDGNRESVRAQAVAFALERLAGLIENGGDA
                     V"
     gene            complement(18066..19634)
                     /gene="msrAB"
                     /locus_tag="WGQ68_001022"
     CDS             complement(18066..19634)
                     /gene="msrAB"
                     /locus_tag="WGQ68_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional peptide-methionine (S)-S-oxide
                     reductase MsrA/peptide-methionine (R)-S-oxide reductase
                     MsrB"
                     /protein_id="HEZ0714842.1"
                     /translation="MKHRTFFSLCAKFGCLLALGACSPKIVDAGAATVPHTLSTLKTA
                     DNRPASVYLKKDKPTLIKFWASWCPLCLSELGQTEKWAQDAKFSSANLITVASPGFLH
                     EKKDGDFQKWYAGLNYPKLPVVTDNGGTIAQSLNISVYPSWALIGKDGDVQRIVKGSI
                     NEAQALALIRDPNADLGSLKHSFYKPDTQKKDSKIMNTRTIYLAGGCFWGLEAYFQRI
                     DGVVDAVSGYANGNTKNPSYEDVSYRHTGHAETVKVTYDADKLSLDDILQYFFRVVDP
                     TSLNKQGNDTGTQYRSGVYYTDPAEKAVIAAALKREQQKYQLPLVVENEPLKNFYDAE
                     EYHQDYLIKNPNGYCHIDIRKADEPLPGKTKTAPQGKGFDAATYKKPSDAELKRTLTE
                     EQYQVTQNSATEYAFSHEYDHLFKPGIYVDVVSGEPLFSSADKYDSGCGWPSFTRPID
                     AKSVTEHDDFSYNMRRTEVRSHAADSHLGHVFPDGPRDKGGLRYCINGASLKFIPLEQ
                     MDAAGYGALKSKVK"
     gene            19779..21044
                     /gene="ftsY"
                     /locus_tag="WGQ68_001023"
     CDS             19779..21044
                     /gene="ftsY"
                     /locus_tag="WGQ68_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEZ0714843.1"
                     /translation="MFSFFRRKKKQETPALEEAQIQETAAKAESETDQVIENIKEDAE
                     SLAESVKGQVESAVETVSGAVEQVKETVAEMLSEAEEAAEKAAEQVEAAKEAVAETVG
                     EAVGQVQEAVATTEEHKLGWAARLKQGLTKSRDKMAKSLAGVFGGGQIDEDLYEELET
                     VLITSDMGMEATEYLMKDVRDRVSLKGLKDGNELRGALKEALYDLIKPLEKPLVLPET
                     KEPFVIMLAGINGAGKTTSIGKLAKYFQAQGKSVLLAAGDTFRAAAREQLQAWGERNN
                     VTVISQTTGDSAAVCFDAVQAAKARGIDIVLADTAGRLPTQLHLMEEIKKVKRVLQKA
                     MPDAPHEIIVVLDANIGQNAVNQVKAFDDALGLTGLIVTKLDGTAKGGILAALASDRP
                     VPVRYIGVGEGIDDLRPFDARAFVDALLD"
BASE COUNT         4536 a         5274 c         6346 g         5092 t
ORIGIN      
        1 aaaagaatga cgaattttag gtttctgttt ttggttttct gtccttgtgg gaatgatgaa
       61 attttaagtt ttaggaattt atcgaaaaaa cagaaaccgc tccgccgtca ttcccgcgca
      121 ggcgggaatc taggtctgtc ggtacggaaa cttatcggga aagcggtttc ttgagatttt
      181 gcgttctgga ttcccacttt cgtgggaatg acgggatgta ggttcgtggg aatgacggtt
      241 taggtatttt tatagaaagc cgtaggtggt gtttctatgc aaccggcagt tgattcattg
      301 cggcaggttg acggcaggtg cttggtgttg atgttggtgg tggtgtcggt ggcggctttg
      361 atggtgtcgt ctttggcggc gcgctcaacc ggctgtccgc agaaccattt taccgaaccg
      421 tcttgacgct tggcccacag ggagagtttt ttgtctttga tttcgttgtt tacgttgctt
      481 gaagccatta tgtcagacgg tattgcccgg gcagctttat tcgtacactt tcagcagctc
      541 gacttcaaat atcaaagtgg cgtgcggagg aatcacgccg cccgcgccgc gtgcgccgta
      601 gcccatttcc gaagggatgg tcagcttgcg tttgccgcct tccttcatgc cgccgaagcc
      661 ttcgtcccag cctttgatga cttgtccgac gccgagcgtg atggtcagcg gctggcggcg
      721 gtcgaggctg gagtcgaatt tggttccgtt ttccagccaa cctgtgtaat gcacggtaat
      781 ctctttgcct ttaactgctt cttttccgaa gccttcctgc aaatcttcaa taatcaggct
      841 gcccatattt gtcctttcgt tgcttgttgg tcaaaacggc aagggtaaca taccgtccgg
      901 cgaagtcaaa tgccgctcaa acgtcagctg catcggtgca gctgaaacgg ctgtgtttgt
      961 ttgactgttt tatttttttc gtaaaggttc catgcttttt catggaaata gaaaacgacg
     1021 gtgttgatta gcggttcgac cagcgcaact gctcccgata cgcctatact gcccgtcagt
     1081 acataggtta cactgaaggc gacgctgaaa tgcagtgcgg caaaagtcag ggttttaagc
     1141 atcatcctct cccggattgg acattgacgg agagatgata aagattatca taaggctgcg
     1201 cggtttaaat ttgctatttg ttgttagtgt agataaatcg ttttttaaat aaggatagga
     1261 attatgaatc ataaaaagat agtcgtattg gatgcggata ctttgcccgg ccgggttttt
     1321 cattttgatt ttccgcacga gcttgcggtt tacggtacga caggtgcgga tgaaacggca
     1381 gaacgggtgc gcgacgcaca tattgtcatt accaataagg taatgatttc cgccgacatt
     1441 attgcggcta atccgcagtt ggagctgatt gccgtcagtg cgaccggtgt gaacaatgtc
     1501 gatattgggg cggcaaaggc ggcgggcgtt gcggtatgca atgtccgcgc atacggaaac
     1561 gaatcggttg cggaacacgc ctttatgctg atgattgcct tgatgcggaa tttgcccgcc
     1621 tatcagcgtg atgttgcggc aggattatgg gagaagtcgc catttttctg tcattacggc
     1681 gcgccgattc gggatttgaa cggtaaaacg ctggcggttt tcggacgcgg caatatcggg
     1741 aggacgctgg ccggatacgc gcaggcattc ggtatggggg tggtgtttgc cgaacacaaa
     1801 cacgcgcccg acgtgcgcga aggctatgtt tcctttgaag atgcggtacg ggctgctgat
     1861 gtgttgtcgc tgcactgtcc gctaaacgcc caaactgaaa atatgatagg cgaaaacgaa
     1921 ttgcggcaga tgaagcctgg cgcggtttta atcaattgtg ggcgcggcgg gctggtggat
     1981 gaaaacgcgc tgcttgccgc actcaaatac gggcagatcg gcggggctgg tgtcgatgtt
     2041 ttgacggagg agccgccaag gggcggcaat cccctgctga atgcacgact tcccaatctg
     2101 attgttacgc cgcataccgc gtgggcgagc cgcgaggctt tggacaggct gttcgatata
     2161 ttgttggcga acattcacgc ctttgtgaaa ggcgaggcgc aaaaccgggt agtttgagct
     2221 tgtcgggatg acggaaaaca atgccgtctg aacgcccaaa gggttcagat ggcatttttt
     2281 gagatttcta cttaaccgac tttgtcgccc ggttgcgcgc ctgcatccac atctaagagc
     2341 ttcagtttgc cgtctgccgt ggcggcactc aaaatcatgc cttcggatac gccgaatttt
     2401 gccattttgc gcggggcgaa gttggcgacg gcgatgacca tccttccatt taactcggca
     2461 gggttgggat aagacgcggc gatgccggag aagatgatgc gtttttcaaa accgaaatcg
     2521 aggtcgaatt ttaagagttt ggtgctgcct tcgacggctt cgcagttcaa tactttggcg
     2581 acgcgcatat cgattttcat aaagtcgtcg aaactcgcct gttcggcaac tttttcgtat
     2641 ttgctctctt cggcggcagg cacggctgcg gcggcgatgc tttgtttgtt ggcttcgatt
     2701 aaatcgtcca cttgtttttg ctccactcgt tgcattaaat gttcgtattt gttgatggcg
     2761 tgtttgccca aggtttcgcg tgtatttgcc caagtgatgg cttccaaatt caggaatttg
     2821 gcggcgtttg cggcggtttg cggcaagacg ggggcgaggt aggcggtcaa catggtgaag
     2881 gcgttgatga gctcgctgca cacttcgtgc aggcgttcgt cttgaccttc ctgtttcgcc
     2941 agttcccacg gcttgttggc atcaacgtat tcgttgacaa tgtctgccaa ggccatgatg
     3001 tcgcgcaggg ctttggcgta ttcgcggctt tcgtattgct cggcgatggt gtcgctttcg
     3061 gcggcaagtt ttgccagcaa tgcgctgtcg gcaacgtctt tcagacggcc ttcaaagcgt
     3121 ttggcgataa aacctgaggc gcgggcggcg atgttgacgt atttgccgac aaggtcgctg
     3181 tttacgcggc tgataaagtc ttgcaggttc aaatcgatgt cttcgatttt gctgttgagt
     3241 ttggcggcga tgtagtagcg catccactcg gggtttaaac cttgttccag ataggatttg
     3301 gcggtgataa acgtgccgcg cgatttggac attttttgtc cgtcgacggt taggaagccg
     3361 tgtgcgtaca cgccggtcgg ggcgcggtgg ccggagaaat gcagcatggc aggccagaac
     3421 agggcgtgga aatagaggat gtctttgccg atgaagtggt acatctcggt ttggctgtcg
     3481 gctttgaagt attcgtcaaa atcgacgccg atgcggtcgc acaggttttt aaacgacgcc
     3541 atgtagccga cgggcgcgtc cagccagacg tagaagtatt tgcccggcgc atcggggatt
     3601 tcaaaaccga aatacggcgc gtcgcgggaa atgtcccagt cggacagtgt ggtttcttcg
     3661 ccttcgccca gccattcttt cattttgttg agggcttctg gttgcagatg gggcttgccg
     3721 tcgtgcgggt tgttgccgga agtccaagct ttgaggaagt cggcgcattc gcccagtttg
     3781 aagaagaagt gttcggattc gcgcaattcg ggtttggcac cggaaacggc ggaatacggg
     3841 ttaatcagtt cggtcgggga ataggtcgtg ccgcagactt cgcagttgtc gccgtattgg
     3901 tcttgggcgt ggcatttcgg gcattcgcct ttgacgaagc ggtcgggcag gaacatttgc
     3961 ttttcaggat cgaaaagctg ctcgatgacg cggctctcaa tcttgccgtt ggctttcagc
     4021 gcgcggtaaa tgtcttggga aaactgtttg ttttcagggg aatgggtgct gtaataattg
     4081 tcgtaaccga tgccgaaatc ggtaaagtcg gcgaggtgct cttcgcgcac tttggcaatc
     4141 atgtcttcgg gcgcgatgcc ttgtttttgc gcggcaagca tcacgggcgt gccgtgggtg
     4201 tcgtcggcgc agcagtagtg gcattcgtgg ccgcgcagtt tttggaagcg aacccaaacg
     4261 tcggtttgga tgtgttcgac catgtggccg aggtggatgc tgccgttggc atagggcagg
     4321 gcggaggtaa ctaagatttt gcgtgtcata ttgtgctttg caaacaatgg gtaaaggcgg
     4381 attataccgc aaatcaaacg gggaaatgcc gtctgaagcc tgaaaaatca ggcttcagac
     4441 ggcatgttcg caaacaatcc ggacttattc gaccgtaacg gatttcgcca ggttgcgcgg
     4501 tttgtccaca tcagtaccgc gtgcgagggc gacatggtag gaaaggagct gcacggggat
     4561 agtatgcacg acgggggata attctccgac atggcgcggt gcgcggataa cgtgcacgcc
     4621 ttcggtagca ttaaaattgc tgtcgaggtc ggcaaagacg aaaagttcgc cgccgcgcgc
     4681 gccgacttcc tgcatattgg ctttgacttt gtccaacagg ctgtcgttgg gtgcgatgac
     4741 gacgacgggc atgttttcgt ccaccaaggc aagcggcccg tgcttcagtt cgccggcagg
     4801 ataggcttcg gcgtggatgt aggtgatttc cttcagtttt aacgcgcctt cgagggcaat
     4861 cgggtaatgg atgccgcgcc ccaaaaacag cgcgctggtt ttcttggcaa actgttgcgc
     4921 ccaggcggca atttgcggtt cgaggtttaa agcgtgctgc acgctgccgg gaagctggcg
     4981 gagttcttcg gtgtaacgtg cttcgtcttc ttcggaaacc aaaccgcgca ctttcgccag
     5041 cgttaccgcc aaaccgaaca gcgcaaccag ctgcgtggta aatgctttgg ttgaggcgac
     5101 gccgatttcc gcaccggcac gggtataaag cacgaggctg ctttcgcgcg gcagggcgga
     5161 ttccatcacg ttgcagatgg agaggctgtg gcggtgtccc aaggattttg cgtatttcaa
     5221 cgcctccatc gtgtccagcg tttcgccgga ttgggaaatg gtaatgacca gttggtcggg
     5281 atcggcaatc acgctgcggt atcggtattc gctggcgatt tcgacatcgg ccgggatttt
     5341 ggcgatggat tccaaccaat atttggcggt cagcgcggcg taataggaag tgccgcaggc
     5401 aaggattttg acgctgcgga tgctttcaaa cacgcttttg gcgtttttgc cgaagttttc
     5461 agggatgaag ccgccgtcga ggaaaacttc cgccgtatcc gcaatcgcgc gcggctgctc
     5521 gtggatttcc ttttgcataa agtggctgta cggccccagc tccaaagagg cgagcgagag
     5581 ttcggatacc ttgactttgc gttcggcagg caggccgctt ttatcggtca gccgtttgat
     5641 gccgtctgaa gccagcagcg cgatgtcgcc gtcttcgagg tacgccacgc ggcgcgtaaa
     5701 ggcgatgacg gcggatacgt ccgaagcgat aaaggtttca tcgtcgccca aagcgaccaa
     5761 aagcgggcag cccatacgcg ccacaactaa ttcatcaggc ttgtcttggg caataaccgc
     5821 gatggcgtat gcgccgtgga aacgtttgac cgcttcctgc acagcttcaa acagcctgcc
     5881 gccgttttgc gcgtattcgt gattgatgct gtgtgcgatg acctcggtat ctgtttgaga
     5941 ttcaaaacgg tatcccaaac cttccaagcg tttgcgttcg ctttcaaagt tttcgatgat
     6001 gccgttgtgg acgaccgcaa tcatcccgcc gctgatgtgc gggtgggcgt tcggctcggt
     6061 aacgccgccg tgtgtcgccc agcgcgtatg cccgatgccg atgccgccgc tgatgccttt
     6121 ttcgcgtgcc gcgtcctcca taagctgcac gcgtccgacg cggcgcacgc gtttgatttt
     6181 gccgtcggtg ttgacggcaa tgcctgatga gtcataaccc cggtattcga ggcgtttgag
     6241 accgtcggtc agaaaatcga cgacgttgtg atgggcgcgg atggcgccga cgataccgca
     6301 cataactgtt ccttagtatc cggttgaaaa aaaacaggcg cggacggctc ccgtgccgca
     6361 ccttcctctt cggattataa accgcctccc gcgccgaaaa acagcaaaat gccgcctgaa
     6421 ggcttgggct tgctcaaaaa agggagggat ttccccgcct gaccgggatg ggcgttcaga
     6481 cggcattgcc tgccgccggt ttatatagtg gattaaattt aaaccagtac ggcgttgcct
     6541 cgccttgccg tactatctgt actgtctgcg gcttcgtcgc cttgtcctga tttaaattta
     6601 accaactata gtttttgcaa atcaatattg acaagctgaa aaaaaaaaac aatatactcg
     6661 ctcggtctta atgttaacgg agtatggaaa tgaaacaaat gcttttagcc gtcggcgtgg
     6721 cggcggtgtt ggcaggctgc ggcaaggatg ccggcggtta cgagggttat tggcgcgaaa
     6781 agtcggacaa aaaagagggt atgattgccg tcaaaaaaga aaaaggcaat tacttcctta
     6841 ataaaatcaa cgtggttaca ggtaaggaag aatctatgct tttgtctgaa aaagacggcg
     6901 cgctttcgat aaacgcgggg ataggggaaa tcccgatcaa actttccgac gacgggaaag
     6961 agctgtatgt cgaacgcagg cagtatgtca aaatcgatgc ggcgatgaag gacaaaatca
     7021 tcgcccatca gaaaaagtgc ggacaaacgg cacaggcata tctcgacgcg cgaaatgcgt
     7081 tgccgtcaaa ccaaacgtat cagcagcgtc aggcggcgat cgagcaattg aaacggcggt
     7141 ttgaagccga gtttgacgaa ttggaaaaag aaatcaaatg caacggcaaa ccgacattgt
     7201 tgttttagcg taggacaacc gggaggatgc cgccgtccga atcggatgtg tggtttccgt
     7261 accggtacgg gcgggcagga atgtccgctt tttttgttcg gacgcgtttg aatatccgtc
     7321 cgattccgtc cgtttgcaaa gggtattttt gttcgggcgg aaattatagt ggattaacaa
     7381 aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctgaag
     7441 caccaagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     7501 tttttgttaa tccactataa ttccgtcaaa taagaaagga attttgtgcc tgcggtatcg
     7561 caaaacttcg ccttaatgcg cccgattgcc tagggatggg cttcagatgg cattgttttc
     7621 cggtttacgg gcggtattcg ggcttcatac cgttgggcag aagctgccag acatatcccg
     7681 tggttttctg tttgccggca agttcgccgg cttcgtcgcc gtatccccaa aaataatcca
     7741 cgcgcaccgc gcctttaatc gcgctgccgg tatcctgcgc cataatcagg cggttgaggg
     7801 ctttgcgggt aaccggatgg gcggtggcga caaataaggg cgcacccaag gtaatgtagt
     7861 gccggtcgac tgcgccggca tattccccca tcagcggcgt gcccagtgcg ccgacaggac
     7921 cgtcatttct gcttccggta agctctcgga aaaagatata gctggggttt tgacccaaaa
     7981 cttcggcgag gcgttgcgga ttttgccgca tataagactt aatgccctgc atggaggttt
     8041 gtccgagttt gaggtagccc ttatccgcca tgtattttcc gatggaaaca tatggatgct
     8101 cgtttttgtc ggcatagccg atgcggatgt atttgccgga cggggttttc agacggcccg
     8161 agccttggat gtgcataaaa aagagttcga cggggtcttc ggcgtaaccg agtatcggtg
     8221 ctttgccgtc aagcgcgccg ccgttgattt ggtttcgcgt gtggtagggg aggaagcggc
     8281 ttccttcaaa cctgcccttg attgccgttg tgcgtgcggt gatggggaat cgggagaggt
     8341 cggcggtgtg cgtgccgccg gcattgtcga ttgtgccgct gtttttcccc gtctgcctga
     8401 tgcggacaag ggcttttccg ctccgcaaac cggcaggcag ggggacggag ataaaatcgt
     8461 cgggaatacc gtaaatcggg aagcgggctt gtgccgtccg cctgtcgtcg cccttcagca
     8521 ccggttcgta atagccggta accgtaccgg caaggcttcc gttgcctgca atctgccacg
     8581 gcgtgaaata gcgttcaaaa aactgttttg cctgaaagga atggacgggg gtttgaaagg
     8641 cttgggcgca cacatcctgc cagccttggc ggtttttcaa attggcgcag ccgaggcgga
     8701 aggattgcag gcttttggcg aaatcctgcg ccgcccagtg gggcagggac aggtgcggta
     8761 caacggtata gacggccccg ccgccgccga ccgtcgttcc ggcggggtcg gggatgccga
     8821 ccggccggtc cgggccgttg atgacggatg tgtcgggttg cggaaaggtt tggatgctct
     8881 tgctttggca ggcggcgagg atggcggcgg cgatgccgta cagggcggcg cggaataggt
     8941 attttttcat aatggacaat gttgccggca gtaataagaa agatggtttc gggcggcgtt
     9001 gcggcagccg tggagagggg attttaacac agggcgcagc tgcagcctgc ggaactttcc
     9061 gccgcgcggt actgcagata aaaataactt gcatttgtat ttacaagcaa tgaaaatatt
     9121 ccgataatat attattcatc atccttgttc gttcgcgttt atgctggtcg cttttttaat
     9181 tatgttgcgc gagggtattg aagccgcgct cattgtcggc atcgttgccg gttttctgaa
     9241 acagtccgga cattccaaac tgatgcctaa ggtctggttc ggggtcgtcc ttgcttcttt
     9301 gatgtgtttg gggctggggt acggcatcca ttcggcaacg ggcgagattc cccagaagca
     9361 gcaggagttc gtcgtcggca ttatcggttt ggttgctgtc gcgatgctga cttatatgat
     9421 tttatggatg aaaaaggcgg cgcgttcgat gaagcagcag cttcaggatt ctgtacaggc
     9481 ggctttgaac cgtggcagcg gtcaaggatg ggccttggtc ggtatggcgt ttcttgccgt
     9541 ggcgcgcgaa ggtttggaga gcgttttttt cctgcttgcc gtattcaaac agagcccgac
     9601 gtggcagatg ccggccggcg cggtagcggg gggtttggct gccgccgtga ttggcgcgtt
     9661 gatttatcag ggtgggatgc gcctgaatct ggcgaagttt ttccgttgga caggggcgtt
     9721 tctgattatc gttgccgccg gtctgcttgc cggctcgctg cgcgcgctgc atgaggcagg
     9781 tatttggaac gcgcttcagg atattgtgtt cgactcatca aaatatttgc acgaagacag
     9841 cccgttgggc gtgctgctcg gcggattttt cggctatacc gaccatccga cgcagggcga
     9901 ggccttggtt tggctgctgt accttattcc cgtcataact tggtttttgt gcggcagcag
     9961 gccgtctgaa actttaaccc gtaaagagga gctgaaatga gaaaattcaa tttgaccgca
    10021 ttgtccgtga tgcttgcctt aggtttgacc gcgtgccagc cgccggaggc ggagaaagct
    10081 gcgccggccg cgtccagtga ggcgcaaacc gccaacgagg gcggttcggt cagtatcgcc
    10141 gtcaacgaca atgcctgcga accgatggaa ctgaccgtgc cgagcggaca ggttgtgttc
    10201 aatattaaaa acaacagcgg ccgcaagctc gaatgggaaa tcctgaaagg cgtgatggtg
    10261 gtggacgagc gcgaaaacat cgcccccgga ctttccgata aaatgaccgt caccctgttg
    10321 ccgggcgaat acgaaatgac ttgcggtctt ttgaccaatc cgcgcggcaa gctggtggta
    10381 accgacagcg gctttaaaga caccgccaac gaagcggatt tggaaaaact gtcccaaccg
    10441 ctcgccgact ataaagccta cgttcaaggc gaggttaaag agctggtggc gaaaaccaaa
    10501 acctttaccg aagccgtcaa agcaggcgac attgaaaagg cgaaatccct gtttgccgac
    10561 acccgcgtcc attacgaacg catcgaaccg attgccgagc ttttcagcga actcgacccc
    10621 gtcatcgatg cgcgtgaaga cgacttcaaa gacggcgcga aagatgccgg atttaccggc
    10681 tttcaccgta tcgaatacgc cctttgggtg gaaaaagacg tgtccggcgt gaaggaaatt
    10741 gcagcgaaac tgatgaccga tgtcgaagcc ctgcaaaaag aaatcgacgc attggcgttt
    10801 cctccgggca aggtggtcgg cggcgcgtcc gaactgattg aagaagtggc gggcagtaaa
    10861 atcagcggcg aagaagaccg gtacagccac accgatttga gcgacttcca agccaatgtg
    10921 gacggatcta aaaaaatcgt cgatttgttc cgtccgttga tcgagaccaa aaacaaagcc
    10981 ttgttggaaa aaaccgatac caacttcaaa caggtcaacg aaattctggc gaaataccgg
    11041 actaaagacg gttttgaaac ctacgacaag ctgggcgaag ccgaccgcaa agcgttacag
    11101 gcctctatta acgcgcttgc cgaagacctt gcccaacttc gcggcatact cggcttgaaa
    11161 taagccgcaa gcgttcagac ggtatttggc ggcagatacc gtctgaagtt ttatctgcaa
    11221 aaccggaaag gtattgaaaa aggcggaggt ttccgttccc cgcgttccgc tgtatcggat
    11281 acagagttta aacaaaggat aaaacatgag tacgaacaac ccgacacaac cgaccaggcg
    11341 cactcttttt aaaaccgcga tcgcagctgg agcagtcggc gcaatcggag gttatctcgg
    11401 cggcaaaaaa cagggcgaaa ccgccgaacg caccgccgaa agccaacact cgccccaagc
    11461 ctatccctgc tacggcgaac atcaggcagg catcgttacg ccgcagcagg cgttttcgat
    11521 tatgtgcgcc ttcgacgtaa ccgcgcaaag tgccaagcag ctggaaaacc tgttccgcac
    11581 gctgaccgcc cgcatcgagt ttctcaccca aggcggcgaa taccaagacg gcgacgacaa
    11641 acttccaccc gccggcagcg gcattttggg caaagccttc aaccccgacg ggttgaccgt
    11701 taccgtgggg gtgggcagca gcctgtttga cggccggttc ggactcaaag acaaaaaacc
    11761 ggttcatttg caggaaatgc gcgacttctc caacgataag ctgcaaaaaa gctggtgcga
    11821 cggcgatttg agcctgcaaa tctgcgcctt cacccccgaa acctgccaag ccgccctgcg
    11881 cgacatcatc aaacacaccg tccaaaccgc cgttatccgt tggagtatcg acgggtggca
    11941 gcctaaatcc gaacccggcg cgatggcggc gcgcaacctg ttgggcttca gggacggcac
    12001 gggcaacccc aaagtttccg atcccaaaac tgccgacgag gttttgtgga cgggggtggc
    12061 cgccaacagc ctcgacgaac cggagtgggc gaaaaacggc agctatcagg cagtccgcct
    12121 tatccgccac tttgttgagt tttgggacag gacgccgctt caagagcaaa ccgacatttt
    12181 cgggcggcgc aaatacagcg gcgcgccgat ggacggcaaa aaagaagccg accaaccgga
    12241 ttttgccaaa gaccccgaga ggaataccac gcccaaagac agccatatac gcctggcgaa
    12301 tccgcgcgat cccgagttcc ttaaaaaaca ccgcctcttc cgccgcgcct acaactattc
    12361 gcgcggactc gcctcaagcg gacagcttga tgtcgggctg gtgttcgtct gttatcaggc
    12421 aaaccttgca gacggcttca tcttcgtgca aaacctcctc aacggcgaac cgctggaaga
    12481 atacatcagc cccttcggcg gcggctattt cttcgtgctg cccggcgtgg aaaaaggcgg
    12541 ctttttgggg caagggctgc tgggcgtata aatccgccat ataaaaaacg ccgtccgaac
    12601 cttgccaaac gggttcggac ggcgtttctt gtttttgggc ggtcaggctt ttttgacgaa
    12661 ttcggatttt aaattcatcg cgctgccgtc gattttgcag ccgatgttgt gatcgccttc
    12721 ttgcaggcgt atgcctttga cttttgtgcc ttgtttgatc accatcgagc tgccttttac
    12781 cttgaggtct ttgatgagga tgacggtatc gccgttttgc agcactgcgc cgttggcatc
    12841 gagcacttga gccgcaaggt cggcggcgga ttcggtttca ttccattcgt gggcgcattc
    12901 ggggcagatg tattgtccgc cgtcttcata ggtgtattcg gaggcgcatt gcgggcatgg
    12961 gggtaatgac atggttttcc gtccttatcg gatgtttgtt tttgggatgc cgtctgaaac
    13021 ctgaaaccgg cttcagacgg catagcttta ttgtttgtct ttttcaggac gcacccaacc
    13081 ttcgatgacg gtttggcggg cgcgggcgag ggcgagtttg ttgtcttcga cattgcgggt
    13141 aatcgtgctg cccgcgcctg tggttacttt gttgccgatt ttgactgggg agaccaagac
    13201 gcagtttgaa ccgatgcgga cttcgtcgcc gatgacggtt ttgtgtttgt gcacgccgtc
    13261 gtagttggca ataatcgtgc ctgcgccgaa gttggttttg ctgccgactt cggcatcacc
    13321 aatgtaggtg aggtggttgg ctttggtgcc gttgccgatg gcggcgtttt tgacttcgac
    13381 gaagttgccg atgtgtacgt cggcggctag tttggcttgc ggacgcaggc gggcgtatgg
    13441 gccgattcgg ttgttttcgc cgacttcgca gctttcgagg tgggagaagg gggcgatttt
    13501 gctgtttgcg ccgattttgg cgtttttgat gacgcagttt gcgccgattt cgacgttgtc
    13561 gccgagctcg atttcgcctt caaagataca gtttgcatca atcacgacat cttgtccgtg
    13621 tttcagacga ccccgcaaat cgaaacgtgc cggatcgcgc agggttacgc ctgctttgag
    13681 caattcttgc gcctgttcgg tttggaagat gcgttcgagt tcggcgagct ggagtttgtt
    13741 gttcacgccg gcggcgaggt aggaggcgcg cacttggacg ggatggactt taataccgtc
    13801 ggcaacggct ttggcgatga ggtcggttaa ataatattcg ccttgggcat tgttgctgga
    13861 aaggctgttc agccagtttt ccagtttggc gttgggcagg acgagaatgc ctgtgttggt
    13921 ttctttcact gctttttggg cggtattggc gtctttttct tcgacaatgg cggttacgct
    13981 gccgttgccg tcgcggatga tgcgccccaa gcctgtcggg gcggtgggaa catcagtcaa
    14041 cagcccgact tcgtcgcctg cggcttcgag cagggtttcg agggttttaa cgtcaatcag
    14101 gggaatgtcg ccgtacagta ccagcgtgcg gccttcggcg gaaaggtggg gcagggcggt
    14161 tttgacggcg tggccggtac cgagctgttc ggtttgttca acccaaacga catcgcgttt
    14221 gacggtgtcc aagacttgct ctttgccgtg gccgatgacg acgcagatgt tttgcggatt
    14281 cagtgcggct gcggtgtcga taacgcgccc gaccatgggc ttgccgccga tgcggtgcag
    14341 cacttttggc attttggaat acatgcgcgt gcctttgccg gcggcgagga tgacgatgtt
    14401 taaagtgttt tgcggcatga cggtttcctg tgcgatgccg tctgaagcgg cttcagacgg
    14461 catagggtgg gtttatcggt tttgaaattt gggcttttgc cagttctgtc cgcgtacgcc
    14521 ttgttcggaa ccgtcggttt ggtcgggcag cacggcatgg cgttcggggc ggtattggtt
    14581 gtagttcata tttttcgagt agctgccgtc ttggtaataa acgggcgtgc cggcgggata
    14641 tttttgacgg acggcggtct tgccgttgcc gtcttgataa gtttcccacg cgcagcccga
    14701 caaaagggcg gcggcagtca tcaggataag gaaagtttta cgcataacat tctttcgtat
    14761 tttcgggagt tgtgcgggta ttgtaatgat tttggcggtg ttctgacaaa gtttctgcat
    14821 actgtgccag ttgcgccata tcgcttacgg aagcatcgat aaagggcagg tcgtgcactt
    14881 ttgcgccgaa ccggacggtt ttcatgccca atgcttttgc ctgatgcagg ttgtccgcgc
    14941 tgtcgtccac cataatgcag cattcgggcg gtacgtccaa aaggcggcag acattgagat
    15001 acgcttgcgg attgggtttg tacagcagtc cgaaatcatc tgtgccgaac agtgcgtcga
    15061 aacggttttc caaaccgagt gcgccgacaa tggcgcggac gtaaaacgac gggccgttgg
    15121 aaaaaaccgc cttgcgccct tttaggcggc tcagggtgtt ttgtgttttc ggcataccgt
    15181 gcagacgggt caaaacctcg tcaatcggat ggctttcgcg caaaaattcg gcgatgtcga
    15241 tttcgggatg gtggatttgc agtccggcga gcgttgcgcc gtagcggtgc caatagtctt
    15301 gacgcaggtc ggacgcggcg gattcggaga gtttgaggcg gcgtgccata tagcgtgtca
    15361 tggcgcggtt gatgagtgtg aagatgccgg catcggcatt gtgcagcgtg ttgtcgaggt
    15421 cgaacagcca cacggtcggg ttttcttgca ttttgaaccg tgaaaatttg ttagaatgtt
    15481 attttacagc gaatagagga ggactcggaa tgaaacggaa aatttggctg ctgccgctgc
    15541 tggcggtttc ggcatacctg caggcgcaga cggaagtcag gctggcggtg cataagtcgt
    15601 tcagcctgcc caaagggctg attgcgcgct tcgagcgggc aaacgatgcg aaggtgtcga
    15661 ttattcaggc gggcggcgcg aacgaaatgc tcaacaaact gattttgagc cgcgccaacc
    15721 cgattgccga cgcggtgtat ggtttggaca acgccaatat cggcaaggcg cgggaaatgg
    15781 gcattttggc ggcggcgcaa cccgaatccg cccccgtcgc ggtcgggctg ccttcggctt
    15841 tggcggtcga ttacggctat gtgtccatca attacgacaa aaaatggttt gaaggcaaaa
    15901 agctgcccct gccgcaaacc ctgcaggatt tgacccgccc cgaatataaa aacctattgg
    15961 tcgtgccgtc ccccgccacg tcgtccccgg ggctgggctt cctgatggcg aacatcagcg
    16021 ggatgggcga agaaggcgcg ttcaaatggt gggcacagat gcggcagaac ggcgtgaagg
    16081 tcgccaaagg ctggagcgag gcgtattaca ccgacttttc gcacaacggc ggcgcgtatc
    16141 cgctggtggt cggttatgcc gccagtccgg cggcggaagt gtatttttcc aaaggcaaat
    16201 acagcgagcc gccgacgggc aacctgtttt taaaaggcgg cgtattccgc caggtcgaag
    16261 gcgcggcagt cttgaagggc gcgaaacagc cgaagctggc ggcaaaactg gtgcaatggc
    16321 tgcaaagtcg ggaagtgcag caggcggttc cgtccgaaat gtgggtttac cccgccgtca
    16381 aaaacacgcg cctgcccgac gtgttccgct tcgcccaagc cccgacgcac accaccgccc
    16441 ccgcgcagcg cgatattgat gcgaaccagc gcggatgggt ttcccgttgg attagaacgg
    16501 ttttgaaata aaacaaacat accgccccgc agggcttcat acggcatttt tacacctgtg
    16561 ccgattacgc cgcacggggc ggatgttcga tcaagaggaa aacaatggac ttcaaacaat
    16621 ttgatttttt acacctgatc agtgtttccg gttgggagca tctggctgaa aaggcgtggg
    16681 cgttcgggct gaaccttgcc gccgcgctgc ttattttttt ggtcggaaaa tgggcggcga
    16741 aacgcattgt cgctgtgatg agggcggcga tgacgcgcgc gcaggtcgat gccacgctga
    16801 ttagtttttt gtgtaatgtt gccaatatcg gcttattgat tttggtgatt attgccgcat
    16861 tgggcagatt gggcgtttcc acaacatccg taaccgcctt aatcggcggc gcgggtttgg
    16921 cggtggcgtt gtccttgaaa gaccagctgt ccaattttgc cgccggtgcg ctgattatcc
    16981 tgttccgccc gttcaaagtc ggcgatttta tccgcgtcgg cggttttgaa ggatatgtcc
    17041 gagagattaa aatggtgcag acttctttgc ggacgaccga caacgaagaa gtcgtgctgc
    17101 ccaacagcgt ggtgatgggc aacagcatcg tcaaccgttc caccctgccg ctttgccgcg
    17161 cccaagtgat agtcggcgtc gattacaact gcgatttgaa agtggcgaaa gaggcggtgt
    17221 tgaaagccgc cgtcgaacac cccttgagcg ttcaaaacga agagcggcag gccgccgcct
    17281 acatcaccgc cttgggcgac aatgccatcg aaatcacatt atgggcttgg gcaaacgaag
    17341 cagaccgctg gacgctgcaa tgcgacttga acgaacaagt ggtcgaaaac ctccgcaaag
    17401 tcaatatcaa catcccgttc ccgcaacgcg acatacacat catcaattct taaacgccgt
    17461 ctgaaagggg agtgggaaat ggacgcgctg cacaccatcg cccgaaacct gacgaaaaaa
    17521 cgtcaaaccg taagctgtgc cgaatcctgc acgggtgggc tgcttgccgc cgtattcaca
    17581 agcgttgcag gcagttcgca atggttcgat caaagttttg taacatacag caacaaagcc
    17641 aaagaagacc gcttgggcgt gttgcccgaa accctgctcg aacacggcgc ggtcagccgc
    17701 caaaccgtct atgagatggc gcgcggcgcg aaagccgtgg cgcaggcgga ttacgccgtc
    17761 ggtatttccg gcatcgccgg tccgggcggc ggcagcgaaa gcaaacccgt cggcacggtt
    17821 tggttcgggt ttgcctttcc gggcggaagt tgcgaagcaa tgcgccgttt tgacggcaac
    17881 cgcgaatccg tccgcgcgca ggcggtcgcc ttcgcgttgg aacggttggc ggggctgatt
    17941 gaaaacggcg gcgatgccgt ctaaacaaaa tctccgtctg gacaaaatcc ccgccggata
    18001 aaaaataccg tctgaaacgt tgcaggtttc agacggtatt ttgtcgggtt aggcggcggt
    18061 gcggcttatt tcactttact cttcaacgcg ccgtagcctg ccgcgtccat ttgttccagc
    18121 gggatgaatt tcaagctcgc gccgttgatg cagtagcgca gtccgccttt gtcgcgcggg
    18181 ccgtcgggga agacgtgtcc caaatgcgaa tcggcggcgt ggctgcgcac ttcggtgcgg
    18241 cgcatgttgt agctgaaatc atcgtgttcg gtaacggatt ttgcatcaat cgggcgcgtg
    18301 aagctcggcc agccgcagcc ggaatcatat ttgtcggcgg agctgaacaa aggttcgccg
    18361 ctgacaacgt ccacataaat gccgggtttg aacaaatggt cgtattcgtg gctgaaggca
    18421 tattcggtcg cgctgttttg ggtaacttgg tattgctctt cggtcagggt gcgtttgagt
    18481 tcggcgtcac tcggtttttt atacgttgcc gcatcgaagc ctttgccttg cggggcggtc
    18541 ttggttttgc ccggcagcgg ttcgtcggct ttgcggatat cgatgtggca gtagccgttg
    18601 gggtttttaa tcaggtagtc ctgatggtat tcctcggcat cgtagaagtt tttcagcggc
    18661 tcgttttcaa caacgagggg cagttggtat ttttgctgct cgcgtttgag ggcggcggcg
    18721 atgacggctt tttcggcggg gtcggtgtag tacacgccgc tgcggtattg cgtaccggtg
    18781 tcgttgccct gtttgttgag gctggtcgga tcaacgacgc ggaagaaata ttgcaggatg
    18841 tcgtctaggc tgagtttgtc ggcatcgtag gtcactttga cggtttcggc gtggcccgta
    18901 tggcggtagg acacgtcttc atagctcgga tttttcgtgt tgccgttggc gtagccggat
    18961 accgcgtcaa ccacgccgtc gatgcgttgg aaataggctt ccaagcccca gaagcagccg
    19021 ccggcgaggt agatggtgcg cgtgttcatg atttttgaat cctttttctg agtgtcgggt
    19081 ttgtagaacg aatgtttcaa gctgcccaaa tcggcattcg ggtcgcggat taacgccaac
    19141 gcctgcgctt cgttgatgct gcctttgacg atgcgctgca cgtcgccgtc tttaccgatt
    19201 aacgcccacg aagggtaaac gctgatattc aggctttggg cgatcgtgcc gccgttgtcg
    19261 gttacgacgg gcagcttggg ataattcaaa ccggcatacc atttttggaa gtcgccgtct
    19321 tttttctcgt gcaaaaagcc cggagacgcg acggtaatca ggttggcgga gctgaatttt
    19381 gcatcttgcg cccatttttc ggtctgtccc aattcggaca gacacaaagg acaccagctc
    19441 gcccaaaatt taatcagcgt cggtttgtct tttttcaagt aaacactggc ggggcggttg
    19501 tccgcagttt tcaaagtgga taaagtgtgc ggcacggtcg cggctccggc atcgacgatt
    19561 ttgggcgaac aagcacccag cgcaagcagg cagccgaact tggcgcaaag ggaaaagaaa
    19621 gtacggtgtt tcattttgag tttcctgtgt ggacggtttg catgattaga cgtttgagat
    19681 gccgaaacct tacagctcgg attttcagac aaccttgccg cgtaaaatac gctacaatac
    19741 gccctatttc aagtttctaa aattaaaagg aaaattcaat gttcagcttc ttccgtcgca
    19801 agaaaaaaca ggaaacgccg gctctcgagg aggctcaaat tcaggaaacc gcagcaaaag
    19861 cagagtccga aaccgatcaa gtaattgaaa atattaaaga agatgctgaa tctttagcag
    19921 aaagcgtcaa agggcaggtc gaatctgccg ttgaaaccgt cagcggtgcg gttgaacagg
    19981 taaaggaaac cgttgccgag atgctgtctg aagcagagga agcggcggaa aaagcagcgg
    20041 aacaagtcga agcggcaaaa gaagccgttg ccgaaaccgt cggcgaggct gtcgggcaag
    20101 ttcaagaagc cgttgcgaca actgaagaac acaagctcgg ttgggcggcg cgtttgaaac
    20161 aaggtttgac caaatcgcgc gacaaaatgg cgaaatcgct ggcgggcgtg ttcggcggcg
    20221 gacaaatcga cgaagatttg tacgaagagc tggaaaccgt gctgattacc agcgacatgg
    20281 gcatggaagc caccgaatac ctgatgaaag acgtgcgcga ccgcgtcagc ctcaaagggc
    20341 tgaaagacgg caacgaattg cgcggcgcgt tgaaagaagc cttgtacgac ctgattaagc
    20401 ctctggagaa acctttggtt ttgcccgaaa ccaaagagcc gtttgtcatc atgcttgccg
    20461 gcatcaacgg cgcgggcaaa accacgtcta tcggtaaact cgccaaatat ttccaagcgc
    20521 agggcaaatc tgtgctgctt gccgccggcg acaccttccg cgccgccgcc cgtgagcagc
    20581 ttcaagcttg gggcgaacgc aacaacgtaa ccgtgatttc gcaaaccacg ggcgattccg
    20641 ccgccgtgtg cttcgatgcc gtccaagccg ccaaagcgcg cggcatcgac attgtgctgg
    20701 ccgacaccgc cggccgcctg cccacgcagc ttcatttgat ggaagaaatc aaaaaagtga
    20761 aacgcgtgct gcaaaaagcc atgcccgacg cgccgcacga aatcatcgtc gtgcttgatg
    20821 ccaatatcgg tcaaaacgcc gtcaaccaag tcaaagcctt tgacgacgca ttagggctga
    20881 ccggtttaat cgtgaccaaa ctcgacggca cggcaaaagg cggcatcctc gccgcgcttg
    20941 cctccgaccg ccccgtcccc gtccgctata tcggcgtggg cgaaggcata gacgatttgc
    21001 gcccgtttga cgcgcgcgcg tttgtggacg cactgctgga ttgagccgaa atgccgtccg
    21061 aaaacagcag accgatgccg tcattcccgc gcaggcggga atccagacct tgggataacg
    21121 gcaatattca aaggttataa aagacccgtc attcccgcgc aggcgggaat ctagacctta
    21181 gaacaacagt aatattcaaa ggttatctga aagtccggga ttctagattc ccactttcgt
    21241 gggaatga
//
LOCUS       DAWFHY010000032        20727 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.032, whole genome shotgun sequence.
ACCESSION   DAWFHY010000032 DAWFHY010000000
VERSION     DAWFHY010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20727
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.032"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(64..240)
                     /locus_tag="WGQ68_001024"
     CDS             complement(64..240)
                     /locus_tag="WGQ68_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714844.1"
                     /translation="MKSRRFFKALLLIAALVGAFYAGMQTQAYLYEDLCLDLGGGKNP
                     GSYPICVIEKVPAR"
     gene            complement(243..1601)
                     /locus_tag="WGQ68_001025"
     CDS             complement(243..1601)
                     /locus_tag="WGQ68_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEZ0714845.1"
                     /translation="MKPLDLNFICQALKLPMPSESKPVSRIVTDSRDIRVGDVFFALA
                     GGRFDAHDFVEDVLAAGAAAVVVSREDCVAMDGALKVDDTLAALQTLAKAWRENVNPF
                     VFGITGSGGKTTVKEMLAAVLRRRFGDNAVLATAGNFNNHIGLPLTLLKLNEKHRYAV
                     IEMGMNHFGELAVLTQIAKPDAALVNNAMRAHVGCGFDGVGDIAKAKSEIYQGLCSDG
                     MALIPQEDANMAVFKTATLNLNTRTFGIDSGDVHAENIVLKPLSCEFDLVCGDERAAV
                     VLPVPGRHNVHNAAAAAALSLAAGLSLNDVAEGLQGFSNIKGRLNVKAGIKGATLIDD
                     TYNANPDSMRAAIDVLARMPAPRIFVMGDMGELGEDEAAAMHAEVGAYARDQGIEAAY
                     FVGDNSVEAAEKFGADGLWFAAKDPLIQVLRHDLPERATVLVKGSRFMQMEEVVEALE
                     DK"
     gene            complement(1726..3372)
                     /locus_tag="WGQ68_001026"
     CDS             complement(1726..3372)
                     /locus_tag="WGQ68_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HEZ0714846.1"
                     /translation="MKQSARIKNMDQTLKNTLGICALLAFCFGAAIASGYHLEYEYGY
                     RYSAVGALASVVFLLLLARGFPRVSSVVLLIYVGTTALYLPVGWLYGAPSYQIVGSIL
                     ESNPAEAREFVGNLPGSLYFVQALFFMFGLTVWRYCVSGGVFADVKNYKRRSKIWLTI
                     LLTLILSCAVMDKIASDKDLREPDAGLLLNIFDLYYDLASAPAQYAAKRAHILEAAKK
                     ASTWHIRHVAPKYKNYVVVIGESARSDYMNVYGFPLPDTPFLSQTKGLLINGYQSTAH
                     ATNLSLPQTLGLPGEPNNNIVSLAKQAGFRTAWLSNQGMLGHFANEISTYALRSDYPW
                     FTQRGDYGKSAGLSDRLLLPAFKRVLIGNAGTKPRLIVMHLMGSHSDFCTRLDKDARR
                     FQYQTEKISCYVSTIAQTDKFLEDTVKILNENKESWSLVYFSDHGLMHVGKGGERTLT
                     HGAWKRQSYGVPLVKISSDDTRREMIKVRRSAFNFLRGFGSWTGIETDELPDDGYDFW
                     GNVPDVQGEGNNLAFIDGLPDDPAPWYAGKGKSTKNTSKK"
     gene            complement(3395..4873)
                     /locus_tag="WGQ68_001027"
     CDS             complement(3395..4873)
                     /locus_tag="WGQ68_001027"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEZ0714847.1"
                     /translation="MFSKLTPLAETDFPPLPCANAAGRLLHSDSRQIKQGDIFVACPG
                     EYADGRSYIPAAVANGAAFVFWDDDGKFAWNPEWKVPNQGIKDLKHRAGMLAAQVYGN
                     VSDGLKVWGVTGTNGKTSITQWLAQAADLLGEKTTIIGTVGNGFWGALEETTHTTPAP
                     VDVQTLLYRFRQQGATAAAMEVSSHGLDQSRVNGVPFRSAIFTNLTRDHLDYHGTMEA
                     YGAIKSRLFYWHGLQHAVINVDDGYGAELIGRLKKDCPDLAIYSYGFSGNADIRITGF
                     TASSDGIEAVFRTPWGEGKCRTRLLGRFNAQNLAACVALLCANGYPLDKVLDVLEKIR
                     PASGRMDCIMNSGKPLVVVDYAHTPDALEKALATLQEIKPQGAALWCVFGCGGNRDRG
                     KRPLMGAAAVQGADKVVVTSDNPRLENPHDIINDILPAVPAPECVEADRAAAIRYAVE
                     QAAANDIILIAGKGHENYQDVQGVKHRFSDLEIVGQALLTRK"
     gene            complement(4898..6643)
                     /locus_tag="WGQ68_001028"
     CDS             complement(4898..6643)
                     /locus_tag="WGQ68_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEZ0714848.1"
                     /translation="MLIKSEYKPRMLPKEEQVKKPMTSNGRISFVLMAIAVLFAGLIA
                     RGLYLQTVTYNFLKEQGDNRIVRTQTLPATRGTVSDRNGAVLALSAPTESLFAVPKEM
                     KEMPSAAQLERLSELVDVPIDVLRNKLEQKGKSFIWIKRQLDPKVAEEVKALGLENFV
                     FEKELKRHYPMGNLFAHVIGFTDIDGKGQEGLELSLEDSLHGEDGAEVVLRDRQGNIV
                     DSLDSPRNKAPKNGKDIILSLDQRIQTLAYEELNKAVEYHQAKAGTVVVLDARTGEIL
                     ALANTPAYDPNRPGRADSEQRRNRAVTDMIEPGSAIKPFVIAKALDAGKTDLNERLNT
                     QPYKIGPAQVRDTHVYPSLDVRGIMQKSSNVGTSKLSARFSSKEMYDLYHELGIGVRM
                     HSGFPGESAGALRNWQKWRPIEQATMSFGYGLQLSLLQLARAYTVLTHDGELLPVSFE
                     KQAVAPKGKRVIKASTAREVRDLMVSVTEPGGTGTAGAVDGFDVGAKTGTARKLVNGR
                     YVDNKHVATFIGFAPAKNPRVIVAVTIDEPTANGYYGGVVTGPVFKKIMGGSLNILGV
                     SPTKPLTAAAVKTPS"
     gene            complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="WGQ68_001029"
     CDS             complement(6704..6967)
                     /gene="ftsL"
                     /locus_tag="WGQ68_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEZ0714849.1"
                     /translation="MNKLNFLLLLAVCVSAFSVVMQQNQYRLNFTALDKAKKQEIALE
                     QDYAQMRLQQARLANHEAIRAAAEKQNLHPPVSGNTFMVAHQR"
     gene            complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="WGQ68_001030"
     CDS             complement(6964..7950)
                     /gene="rsmH"
                     /locus_tag="WGQ68_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEZ0714850.1"
                     /translation="MSGAESYRHITVLLNEAVDALAVREDGVYVDGTFGRGGHSRLIL
                     SRLGDAGRLIVFDKDPQAIAVAEELARSDKRVGVVHGGFASFQTALDGLGIGKVDGAL
                     FDLGISSPQIDDGSRGFSFRFDAPLDMRMDTMRGMSAAEWIAVASEQDLHEVIKNYGE
                     ERFSRQIARAIVAQRAESPIDTTRKLAQIVAQNVRTRERGQDPATRTFQAIRIFINRE
                     LEEVGAVLPQVMCRLKEGGRLAVIAFHSLEDRIVKQFVKKYSQHAPLPRWAAVREADL
                     PEPPLKIVGRALKPGEAEIAANQRARSAVLRVAERTAGSIPEQSQRKTSEWQ"
     gene            complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="WGQ68_001031"
     CDS             complement(7947..8402)
                     /gene="mraZ"
                     /locus_tag="WGQ68_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEZ0714851.1"
                     /translation="MFGGAHELSIDSKGRLAVPAKFRDILSRLYTPAVVVTLESKHKL
                     LMYPVAEWEKVAAQLLNLKVADNPVLRRFQNLLLHNAEILEWDSAGRVLLPAGLRKRV
                     DFDREVVLVGRANRLELWGREQWEAEMVQALDDDPDELAFQLSQTDLQL"
     gene            8681..9898
                     /locus_tag="WGQ68_001032"
     CDS             8681..9898
                     /locus_tag="WGQ68_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0714852.1"
                     /translation="MTAPVPTCNMSGNPINLHKLTLIMPLPLPSPEARQSSLKLQTLI
                     AEKIGKHGNWIPFSRFMELVLYAPQYGYYTGGSHKIGNTGDFITAPTLTSLFAQTLAR
                     QLQELLSQTAGNIYEFGAGTGQLAADLLGSISDGISRYYIIEISPELAARQKNLIQAR
                     APEASQKVVHLTALPEAFDGIIIGNEVLDAMPVEIVRKNEGGSFEHVGVCLDNDRFTY
                     SARPLHDLQLSALASLYFPQTDYPYTSELHPQQYAFIRTLASRLERGGMIFIDYGFDA
                     AQYYHPQRNQGTLIGHYRHHVIHNPFDFIGLADLTAHVNFTDIAQAGTDAGLDLIGYL
                     PQSHFLLNLGITELLAQTGKTDSAAYICEAAAVQKLIDQHEMGELFKVIAFGKNIGID
                     WAGFRFGDICHKL"
     gene            10030..10106
                     /locus_tag="WGQ68_001033"
     tRNA            10030..10106
                     /locus_tag="WGQ68_001033"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10064..10066,aa:Met,seq:cat)
     gene            10114..10189
                     /locus_tag="WGQ68_001034"
     tRNA            10114..10189
                     /locus_tag="WGQ68_001034"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10147..10149,aa:Ala,seq:ggc)
     gene            complement(10471..11292)
                     /locus_tag="WGQ68_001035"
     CDS             complement(10471..11292)
                     /locus_tag="WGQ68_001035"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEZ0714853.1"
                     /translation="MDFLIVLKALMMGLVEGFTEFLPISSTGHLIVFGNLIGFHSNHK
                     VFEIAIQLGAVLAVVFEYRQRFSNVLHGVGKDRKANRFVLNLAIAFIPAAVMGLLFGK
                     QIKEHLFNPLSVAVMLVLGGLFILWVEKRQSWAEPKIADVDALRPIDALMIGVAQVFA
                     LIPGTSRSGSTIMGGMLWGIERKTATEFSFFLAVPMMVAATAYDVLKHYRFFTLHDVG
                     LILIGFVAAFVSGLVAVKALLRFVSKKNYIPFAYYRIVFGIAIIILWLSGWISWE"
     gene            complement(11325..11969)
                     /locus_tag="WGQ68_001036"
     CDS             complement(11325..11969)
                     /locus_tag="WGQ68_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0714854.1"
                     /translation="MKLKHLLPLLLSAVLSAQAYALTEREDYLVLDKPIPQEQSGKIE
                     VLEFFGYFCVHCHHFDPLLLKLGKALPSDAYLRTEHVVWQPEMLGLARMAAAVNLSGL
                     KYQANPAVFKAVYEQKVHLEDRAVAGKWALSQKGFDGKKLMRVYDSPEAAAAASKMQK
                     LTEQYGIDNTPTVIVGGKYRVIFNNGFDGGVHTIKELVAKVREERKRQPPAVQK"
     gene            complement(11972..12835)
                     /gene="ftsN"
                     /locus_tag="WGQ68_001037"
     CDS             complement(11972..12835)
                     /gene="ftsN"
                     /locus_tag="WGQ68_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0714855.1"
                     /translation="MNKFSQSGKGLSGFFFGLILATVIIAGILFYLNQSGQNAFKIPA
                     SSKQPAETEILKPKNQPKEDIQPEPADQNALSEPDAAKEAEQSDAEKAADKQPVADKA
                     DEVEEKADEPEREKSDGQAVRKKALTEEREQTVGEKAQKKDAETVKKQAVKPSKETEK
                     KASKEEKKAEKEKVAPKPTPEQILNSGSIEKARSAAAKEVQKMKTPDKAEATHYLQMG
                     AYADRRSAEGQRAKLAILGISSKVVGYQAGHKTLYRVQSGNMSADAVKKMQDELKKHE
                     VASLIRSIESK"
     gene            complement(12941..14437)
                     /locus_tag="WGQ68_001038"
     CDS             complement(12941..14437)
                     /locus_tag="WGQ68_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HEZ0714856.1"
                     /translation="MSLALVYSRALSGMNAPLVEVEAHLANGLPHFNIVGLPDTEVKE
                     SRDRVRAAIIQSGFEFPAKKITVNLAPADLPKESGRFDLPIAIGILAASGQVAPEKLA
                     EYEFAGELALSGLLRPVRGALAMAWQGMQAKRAFVLPEENAGQAAVMRGITVYGARSL
                     GEVAAHLNGIEPLAQTECSVPQMPSEHGGQPDLCDVKGQHTARLALEIAAAGGHSLLM
                     MGPPGTGKSMLSQRLPGILPPLTEDELVEVWALRSLLPNHQQQLDSNRPFRSPHHSAS
                     AAAMVGGGSDPRPGEISLAHHGVLFLDELPEFDRKVLEVLREPLENGEIHISRAARQA
                     VYPAKFQLVAAMNPCPCGYLGHPVKPCRCTPESVARYRSKISGPLLDRIDLTIEVPSL
                     SAAELMQQEAGERSASVLERVIAARDKQYARQGKVNAALSVSELDTSARIQKEAQEAL
                     GSLLEKLSLSARSFHRIMRVARTLADLAGDEEVGRSHVMKAIGFRRAL"
     gene            complement(14451..14771)
                     /locus_tag="WGQ68_001039"
     CDS             complement(14451..14771)
                     /locus_tag="WGQ68_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003763371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HEZ0714857.1"
                     /translation="MFGKQLFEEVGSKISETIANSPAKDVEKNIKAMLGGAFNRMDLV
                     TREEFDIQQQVLIKTRTKLAALEARLAKLEAAQNPERAALEAAEAAAEEAVAEIRQQT
                     EAGE"
     gene            15254..16780
                     /gene="putP"
                     /locus_tag="WGQ68_001040"
     CDS             15254..16780
                     /gene="putP"
                     /locus_tag="WGQ68_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HEZ0714858.1"
                     /translation="MNPMYITFAIYLVAVLLIGLAAYFSTRNFDDYILGGRSLGPFVT
                     AMSAGASDMSGWLLMGLPGAIYLSGLNEAWIAIGLLVGAYFNWLLVAGRLRVHTEYAN
                     NALTLPDYFFHRFGAGGHLMKVVSALIILFFFTIYCASGIVAGATLFQSLFEGMTYNQ
                     AMWLGAGATIAYTFLGGFLAVSWTDTLQASLMIFALILTPVMVYLGLGGAEQMSAAIQ
                     SVAAGTGKEYGSLFAGTTVIGIISTAAWGLGYFGQPHILARFMAAESAKSLVSARRIG
                     MTWMALCLAGAVAVGYFGIAYFGANPDKVSSMSGNHERIFIALSTLLFNPWIAGIILS
                     AILAAVMSTLSCQLLVCSSAITEDFYKGFLRKNAQQSELVWVGRLMVLAIAVISILIA
                     SDPNSKVLGLVSYAWAGFGAAFGPIVILSVLWKRITAYGALSGMVAGALTVVVWAEWV
                     KKPAQAAGESGLLTMYEIVPGFIVCLIVAVLVSLFNKEPSRETQERFEKADADYRAAR
                     "
     gene            17031..20636
                     /gene="putA"
                     /locus_tag="WGQ68_001041"
     CDS             17031..20636
                     /gene="putA"
                     /locus_tag="WGQ68_001041"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEZ0714859.1"
                     /translation="MFHFAFPAQTALRQAITDAYRRNEIEAVQDMLQRAQMSDEERNA
                     ASELARRLVTQVRAGRTKAGGVDALMHEFSLSSEEGIALMCLAEALLRIPDNATRDRL
                     IADKISDGNWKSHLNNSPSLFVNAAAWGLLITGKLTATNDRQMSSALSRLISKGGAPL
                     IRQGVNYAMRLLGKQFVTGQTIEEALQNGKEREKMGYRFSFDMLGEAAYTQADADRYY
                     RDYVEAIHAIGKDAAGQGVYEGNGISVKLSAIHPRYSRAQHGRVMGELLPRLKELFLL
                     GKKYDIGINIDAEEANRLELSLDLMEALVSDPDLAGYKGIGFVVQAYQKRCPFVIDYL
                     IDLARRNNQKLMIRLVKGAYWDSEIKWAQVDGLNGYPTYTRKVHTDISYLACARKLLS
                     AQDAVFPQFATHNAYTLGAIYQMGKGKDFEHQCLHGMGETLYDQVVGPQNLGRRVRVY
                     APVGTHETLLAYLVRRLLENGANSSFVNQIVDENISIDTLIRSPFDTIAEQGIHLHNA
                     LPLPRDLYGKCRLNSQGVDLSNENVLQQLQEQMNKAAAQDFHAASIVNGKARDVGEAQ
                     PIKNPADHDDIVGTVSFADAALAQEAVGAAVAAFPEWSATPAAERAACLRRFADLLEQ
                     HTPALMMLAVREAGKTLNNAIAEVREAVDFCRYYANEAEHTLPQDAKAVGAIVAISPW
                     NFPLAIFTGEVVSALAAGNTVIAKPAEQTSLIAGYAVSLMHEAGIPTSALQLVLGAGD
                     VGAALTNDARIGGVIFTGSTEVARLINKALAKRGDNPVLIAETGGQNAMIVDSTALAE
                     QVCADVLNSAFDSAGQRCSALRILCVQEDVADRMLDMIKGAMDELVVGKPIQLTTDVG
                     PVIDAEAQQNLLNHINKMKGVAKSYHEVKTAADVDSEKSTFVRPILFELNNLNELQRE
                     VFGPVLHVVRYRADKLDSVIDQINSKGYALTHGVHSRIEGTVRHIRSRIEAGNVYVNR
                     NIVGAVVGVQPFGGHGLSGTGPKAGGSFYLQKLTRIPEWVAPTLSQIGQADEAALKRL
                     EALVHKLPFNAEEKKAAAAALGHARIRTLRRAETVLTGPTGERNSISWHAPKRVWVHG
                     GSLLQAFCALTELAASGVQAVVEPDSPLASYTADLEGLLLVNGKPETADISHVAALSP
                     LDSARKQELAAHDGALIRILPSENGLDILQVFEEISCSVNTTAAGGNASLMAVAD"
BASE COUNT         4860 a         6002 c         5025 g         4840 t
ORIGIN      
        1 tcgggcctgc gcccgtttga ggttggcttg ccgcccggca aggtttcgga cggcttttgc
       61 agattaacgt gcagggactt tctcaatcac gcaaatcggg taactccccg gatttttgcc
      121 gccgcccaag tctaaacata aatcttcata aagatacgcc tgcgtctgca ttccggcata
      181 aaacgcgccg accagcgcgg caatcaacaa taaggcttta aaaaaacgtc gacttttcat
      241 tttcacttat cctccaatgc ctcgaccact tcttccatct gcataaagcg cgaacctttc
      301 accaacacgg tggcgcgttc gggcaaatcg tggcgcaaca cttgaatcaa cgggtctttg
      361 gcggcgaacc acaaaccgtc cgcgccaaat ttttccgccg cttcgacgct gttgtctccg
      421 acaaaataag ccgcttcgat gccttggtcg cgggcgtacg cgccgacttc ggcgtgcatg
      481 gcggcggctt cgtcctcacc cagttcgccc atatcgccca tcacaaaaat gcgcggcgca
      541 ggcatacgcg ccaacacgtc aatcgctgcc ctcatgctgt cggggttcgc gttgtaggta
      601 tcgtcaatca gggtcgcgcc cttgatgccg gctttgacgt tcagacggcc tttgatgttg
      661 ctgaagcctt gcaaaccttc cgccacatcg ttcagactca aacccgcagc caaagacagc
      721 gcggcggcag cggcggcgtt gtggacattg tggcggccgg gaacaggcag caccacggcg
      781 gcgcgttcgt cgccgcacac caaatcaaat tcgcacgaca acggtttcag cacgatattt
      841 tccgcgtgga catcgccgct atcgatgccg aaagtgcgcg tattcaaatt aagcgttgcc
      901 gttttgaaga cagccatatt ggcatcttct tgaggaatca gcgccatgcc gtctgaacat
      961 aagccttgat aaatctcgct tttcgctttg gcaatatcgc ccactccgtc gaaaccgcag
     1021 ccgacatggg cgcgcatggc gttgttgacc aatgcggcat cgggtttggc gatttgtgtc
     1081 aaaaccgcca gttcgccaaa atggttcata cccatttcaa tcacggcata gcggtgtttt
     1141 tcgtttaatt tcaacaaagt caacggcaat ccgatgtggt tgttgaagtt gcctgccgtc
     1201 gccaaaacgg cattatcgcc gaaacggcgg cgcaataccg cagccaacat ttccttcacc
     1261 gtcgtcttac cgcccgagcc ggtaataccg aacacaaacg ggttcacatt ctcgcgccac
     1321 gcctttgcca gcgtttgcaa tgcggcaagc gtgtcatcga ctttcaacgc gccatccatt
     1381 gcaacgcaat cttcgcgcga aaccacaacc gccgccgcac ccgcagccaa tacgtcttca
     1441 acaaaatcat gcgcgtcaaa ccgcccgccc gccaatgcga aaaacacatc gcctacgcgg
     1501 atgtcgcggc tgtcggttac gatgcgcgac acgggtttgc tttcagacgg catcggaagc
     1561 ttgagggctt ggcagatgaa atttaggtcc agtggtttca tatttacttt cgttaatatt
     1621 cgggcggcgg acacatcggc agcggctgat ttttttatcg cctgttttgc tgtggtaaaa
     1681 cacagattat tttcccattc tcattcggca ttttttctgt acgtatcatt ttttagacgt
     1741 atttttagtc gatttgcctt ttcccgcata ccacggcgcg gggtcgtcgg gcagtccgtc
     1801 gataaaggca aggttattgc cttcgccctg cacatcggga acattccccc aaaaatcata
     1861 gccgtcatcg ggcaactcgt cggtttcgat acccgtccaa ctgccgaatc cgcgtaaaaa
     1921 attaaacgcg ctgcgcctca ctttaatcat ttcgcgccgc gtgtcatcgg acgaaatttt
     1981 aaccagcggc acgccgtagc tttgacgctt ccacgcacca tgtgtcaacg ttcgctcgcc
     2041 gcctttaccg acatgcatca aaccgtggtc ggaaaagtaa accaaagacc agctttcttt
     2101 attttcattc aatatcttaa ctgtatcttc taaaaattta tcggtttgcg cgatggtgga
     2161 aacatagcag gatatttttt cagtttgata ctgaaaccgc cgcgcatcct tatccaaacg
     2221 tgtgcaaaaa tcactgtgcg aacccatcag gtgcatcaca atcagccgag gcttcgtgcc
     2281 tgcatttcct atcaaaaccc gtttgaacgc cggcaacaaa aggcggtcgc tcaaccccgc
     2341 gcttttgcca taatcacccc tttgggtaaa ccacggataa tcgctgcgta gggcataggt
     2401 ggaaatttcg ttggcaaaat gccccaacat tccttgatta gacagccacg ccgtccgaaa
     2461 acccgcctgc ttcgccaagc tgacgatgtt attgttcggt tctcccggca gccccaaagt
     2521 ctgcggcagc gaaagattcg tcgcgtgggc ggtcgattgg taaccgttta tcaacagccc
     2581 tttggtctga ctcaaaaaag gcgtatcggg caatgggaaa ccgtaaacat tcatataatc
     2641 cgaacgcgcg ctctcaccga taaccacaac ataattttta tacttgggcg caacatgacg
     2701 gatatgccat gttgacgctt tttttgctgc ttccaaaatg tgggcgcgct tggcggcata
     2761 ttgtgccggc gcggaagcca aatcgtaata caggtcgaaa atattcaaca acaggccggc
     2821 atcaggttct cgcaaatctt tatcgctggc gattttatcc atcaccgcgc aggacaaaat
     2881 caaagtcaat aatatagtca gccatatttt gctgcggcgt ttatagtttt ttacgtcagc
     2941 aaataccccc cccgatacac aatatctcca aactgtcaag ccaaacatga aaaataatgc
     3001 ctgcacaaaa taaagcgacc cgggaagatt gccgacaaat tcacgcgcct cggcaggatt
     3061 gctttccaat atcgaaccga ctatctgata agacggcgca ccatacagcc agccgaccgg
     3121 caaatatagg gcggttgtgc cgacgtaaat cagtaaaaca actgaagaaa cgcgcgggaa
     3181 accgcgtgcc aataataata aaaatacaac cgaagccaaa gcacccacgg cagaataacg
     3241 gtagccgtat tcatattcca agtgataacc tgatgcgatg gccgcgccaa aacaaaaggc
     3301 taaaagcgcg caaatgccca atgtattttt taatgtctga tccatatttt ttattcgggc
     3361 ggattgtttc atacgatgcc gtccgaatat cccattattt acgagttaac aaagcctgtc
     3421 cgacgatttc aagatcggaa aaacggtgct tcacgccttg tacatcctga tagttttcat
     3481 gccctttgcc ggcaatcagg atgatgtcgt ttgcggcggc ttgttcaacc gcataacgga
     3541 tggcggcggc acggtcggct tcgacgcatt cgggcgcggg aacggcaggc aggatgtcgt
     3601 tgatgatgtc gtgcggattt tccaaacgcg gattgtcgct ggtgacgacg actttatccg
     3661 cgccctgtac ggctgccgcg cccatcagcg ggcgtttgcc tcggtcgcgg ttgccgccgc
     3721 aaccgaatac gcaccacaaa gccgcaccct gcggtttgat ttcctgcaag gtggcgagtg
     3781 ctttttccaa tgcgtcgggc gtgtgggcgt aatcgacgac gaccaagggc ttgccgctgt
     3841 tcatgatgca gtccatacga cctgaagcag gacggatttt ttccagtaca tccaatacct
     3901 tatccagcgg atagccgttg gcgcaaagca aagcgacaca ggcggcgaga ttttgcgcgt
     3961 tgaaccgtcc gagcaggcgc gtgcggcatt tcccttcgcc ccacggggtt cggaatacgg
     4021 cttctatgcc gtctgaagag gcagtgaagc cggtaatgcg gatgtcggcg tttccgctga
     4081 atccgtagct gtaaatggcc aaatcggggc agtctttttt cagacggcct atgagttctg
     4141 cgccgtatcc gtcatctaca ttgataacgg cgtgttgcag gccgtgccag taaaacaggc
     4201 gcgacttgat agcgccgtag gcttccatcg tgccgtggta gtcgaggtgg tctcgggtga
     4261 gattggtgaa gattgcgctg cggaatggca cgccgttgac gcgcgactgg tcaagcccgt
     4321 ggctggaaac ctccatcgcg gcggcggttg cgccttgttg acggaaacgg tagagcaggg
     4381 tttggacatc gacgggggcg ggggtggtgt gcgtggtttc ttccaatgcg ccccaaaagc
     4441 cgttgccgac cgtgccgata atggtagttt tttcacccaa caaatcggcg gcttgcgcca
     4501 gccattgtgt gatggaagtt ttgccgttgg taccggttac gccccagact ttgaggccgt
     4561 ctgaaacgtt gccgtacact tgcgccgcca acataccggc tcggtgtttc aaatctttaa
     4621 tgccttgatt ggggactttc cattcgggat tccacgcaaa ttttccgtcg tcgtcccaaa
     4681 aaacaaaagc cgcgccgttg gcaacggcgg cggggatata actgcgaccg tcggcatatt
     4741 cgcccgggca ggcaacgaaa atatcgcctt gtttgatttg gcggctgtct gaatgcaaca
     4801 aacgccctgc cgcgtttgca cacggcagag gcggaaagtc ggtttcagcc aaaggggtta
     4861 acttgctgaa cataaaacaa tctcgttgat actcggatta agacggtgtt ttgacggctg
     4921 cggcggtcag aggcttggtc ggggaaacgc ccaagatgtt caggctgccg cccataattt
     4981 ttttgaagac cggtcctgtc actacgccgc cgtagtaacc gtttgcagtc ggctcgtcga
     5041 tggttaccgc cacaatcaca cggggatttt tggcaggtgc aaaaccgata aaggtagcga
     5101 cgtgtttgtt gtccacatag cggccattga ccagtttgcg cgccgtaccg gttttcgcac
     5161 ctacgtcgaa accatctacc gcacctgctg tacctgtacc gccgggttcg gttacagaaa
     5221 ccatcaaatc acgaacttca cgcgcggtcg aagctttaat gacacgcttg cctttaggcg
     5281 caaccgcttg tttttcaaaa cttaccggca acaattcgcc gtcatgggtc aagacagtat
     5341 aggcacgcgc caattgcaac aggcttaatt gcagaccata accgaaagac atggttgcct
     5401 gttcgatagg ccgccatttt tgccaattac gcaacgcgcc cgcactttct cccggaaaac
     5461 cggaatgcat acgcacaccg atgcccaatt catgatacaa atcatacatt tctttcgaac
     5521 tgaatcgtgc agacagtttg cttgtaccga cattggaaga tttctgcatg ataccgcgca
     5581 catccaaaga aggataaaca tgggtatcgc gcacctgagc cggtccgatt ttataaggct
     5641 gcgtattcag ccgttcgttc aaatcggttt tgcccgcatc caatgctttc gcaatcacaa
     5701 acggtttgat tgccgaaccg ggttcgatca tatcggttac ggcacggttg cgccgctgtt
     5761 cgctgtctgc ccggccgggc ctgttgggat cgtaggcggg cgtattggcc aaggcgagga
     5821 tttcccccgt gcgggcatcc aaaaccacca ccgttccggc ttttgcctga tggtattcga
     5881 ccgccttgtt caactcttca taggccaagg tctgaatcct ctgatcgagg gaaaggatga
     5941 tgtctttgcc gtttttcggg gctttattgc gcggggagtc caagctgtcc acaatattgc
     6001 cctgccggtc ccgcaaaacg acttccgcgc cgtcttcgcc atgcaggctg tcttcaagcg
     6061 aaagttccaa accttcctga cctttgccgt caatatcggt aaatccgatg acgtgtgcaa
     6121 acaggttgcc catcgggtaa tggcgtttta attctttttc aaatacaaag ttttccaaac
     6181 ccaaggcttt gacctcttcg gcaaccttgg gatcgagctg ccgcttaatc cagataaacg
     6241 acttgccttt ctgttcgagc ttgttcctca aaacatcaat cggaacatcg acaagctcgg
     6301 acaggcgttc caattgtgcg gcagacggca tttccttcat ctctttaggc acggcaaaca
     6361 gggactccgt cggcgcactc aacgccaaaa ccgcaccgtt ccggtccgaa accgtaccgc
     6421 gtgtagccgg caatgtttga gtccgcacaa tccggttgtc gccctgttct ttcaaaaagt
     6481 tatacgttac cgtctgcaga tacagtccgc gggcaatcag accggcaaac aagaccgcta
     6541 ttgccatcag gacgaagctg atccgtccgt tactggtcat cggctttttg acctgctctt
     6601 ctttgggcag catccggggc ttatattcac tcttaatcaa catttttact tctcgttatt
     6661 attatcctga cgcaggaatc cgattccggc acacaggctg cttctatctt tgatgcgcca
     6721 ccataaaggt attgcccgaa accggcggat ggaggttttg tttttctgcc gccgccctga
     6781 tcgcttcgtg gttcgccaaa cgcgcctgtt gcagcctcat ttgcgcataa tcctgctcca
     6841 aggcgatttc ctgttttttc gccttgtcca aagctgtgaa gttgagcctg tactggtttt
     6901 gctgcatcac aacggaaaaa gcggaaacgc acaccgcaag cagcagaagg aaattcaatt
     6961 tgttcattgc cattcagacg tttttctctg tgattgttcc ggtatcgaac cggcagtccg
     7021 ctccgccaca cgcaaaaccg cacttctcgc cctctgattg gcggcaattt ccgcctcacc
     7081 cggctttaat gccctgccca cgattttcag gggcggctcg ggcaaatccg cttccctgac
     7141 cgccgcccaa cgcggcaggg gcgcgtgttg cgaatatttt ttgacaaact gcttcacaat
     7201 gcggtcttcc aacgaatgga aagcaatgac cgccaaacgt ccgccctctt tcagacgaca
     7261 catgacctgc ggcaatactg cccctacttc ttcaagctcg cggttaataa agatgcggat
     7321 tgcctggaag gtgcgcgtcg caggatcctg cccccgctcg cgagtacgga cgttttgtgc
     7381 cacgatctgc gccagcttgc gggttgtatc gattggactt tccgcccgtt gcgcaacaat
     7441 ggcgcgcgca atctggcggc taaaccgctc ttcaccataa ttcttgatta cctcgtgcaa
     7501 atcctgttcc gacgcaaccg ctatccactc tgcggcagac ataccgcgca tcgtatccat
     7561 acgcatatcc aaaggggcat cgaaacgaaa gctgaaaccg cggctgccgt catcgatttg
     7621 cggagacgaa atccccaaat caaacagcgc accgtccacc ttgccgatac ccaaaccgtc
     7681 caatgccgtc tgaaacgaag caaaaccgcc atgcacgaca ccgacccgtt tgtccgaacg
     7741 cgccagctct tctgccacag caatcgcctg cgggtctttg tcgaaaacaa tcaaccgccc
     7801 cgcatcgccc aaacgcgaca aaatcagccg ggaatgccct cccctgccga acgtaccgtc
     7861 cacatagaca ccgtcttcgc gcacggcaag cgcatccacc gcctcattca gcaagaccgt
     7921 gatatgccgg taactttctg ctccactcac aattgcaaat ccgtctgact caactggaag
     7981 gcaagttcgt caggatcgtc atccaaagcc tgaaccatct cagcctccca ctgctcgcga
     8041 ccccaaagct ccaaacggtt ggcacgaccg accaaaacga cttcacggtc gaaatccacc
     8101 cttttcctca gtccggcagg aagcagcacc cggccggcgc tgtcccattc caaaatttcc
     8161 gcgttatgca gcaaaagatt ttgaaaccgc cgcaaaacag ggttatccgc cacttttaag
     8221 tttaaaagtt gcgccgcaac cttttcccac tccgcaacag ggtacatcaa cagcttgtgt
     8281 ttcgactcga gcgttaccac tacggcaggc gtatagaggc gcgacagaat gtcacggaat
     8341 ttggcaggaa cagccaaccg ccccttactg tcgatgctta attcgtgtgc gccgccgaac
     8401 atgacatgtc ccaagccgaa atcaaaatca caagggtaaa agagacactt tgccccacaa
     8461 ttccccacca atcgacacta taagaaattt taaacactcg gtcaaatcag ggcatgaaaa
     8521 ctcattaaca tatctgaaat ttttattcct tttaaaaaca ataagataaa aaatgacgac
     8581 aacggcacgg cgggtgcggt acagaataat cgaaccaata aacaactata tatgattaat
     8641 ttaataatat aaacacaata tatagtatta agataaagcc atgacagcac ccgtaccaac
     8701 gtgtaatatg tcgggaaatc caataaattt acacaagcta acacttatca tgcccctccc
     8761 cctcccctct cccgaagcac ggcaatcctc gctcaaactg caaaccctca ttgccgaaaa
     8821 aatcggtaaa cacggcaact ggattccatt ttcacgtttt atggaattgg ttttatacgc
     8881 tccgcaatac ggctactaca ccggcggcag ccataaaatc ggcaataccg gggattttat
     8941 taccgcaccg accctcacct ctctgtttgc acagacactg gcacgccaac ttcaagaact
     9001 tctatctcaa acggcgggca atatctatga attcggcgcg ggaaccggac agcttgccgc
     9061 cgatttgttg ggcagcattt cggacggcat cagccgttac tatattattg aaatatcgcc
     9121 ggagctggca gcacgtcaga aaaacctgat tcaagcccgc gcaccggaag catctcaaaa
     9181 agttgtccac ttgaccgcac ttcccgaagc gtttgacggc atcatcatcg gcaacgaagt
     9241 actcgatgcc atgcctgtcg aaatcgtccg taaaaatgaa ggcggctcat tcgagcatgt
     9301 cggcgtttgc ctagataatg accgttttac ctattcggca cgaccgctgc acgacttaca
     9361 gctatctgcc ttggcttccc tctattttcc tcaaacagat tatccctaca ccagcgaact
     9421 acatccgcaa caatatgcct ttatccgcac ccttgcctca agacttgaac gcgggggtat
     9481 gatattcatc gactacggtt ttgatgcagc gcagtattac caccctcaac gcaatcaagg
     9541 tactctgatc ggacactacc gacatcacgt tatccacaat ccttttgact tcatcgggtt
     9601 ggccgacctg accgcgcatg tcaactttac cgacattgcc caagccggga cggatgccgg
     9661 attagatttg ataggttacc ttccccaatc ccatttctta ttgaacttgg gcattaccga
     9721 gctattggca cagacgggga aaacggattc ggcagcctac atctgtgaag ctgctgccgt
     9781 tcagaaactg attgaccagc atgaaatggg cgaactgttt aaagtcatcg cattcggaaa
     9841 aaatatcggc atcgactggg caggattccg cttcggcgac atctgccaca aactctaacc
     9901 ctcatgccgc ctgaatccgc ttcagacggc ataaactttt taacatttaa aaacagtcaa
     9961 ctaattcaaa attaaaaaat acggcttgtc aaaaaaacag aaaaacatat aatagcgtct
    10021 tcacgaaacg gcgaattagc tcagtcggtt agagcagagg aatcataatc cttgtgtccg
    10081 gggttcgagt ccctgattcg ccaccaaatt ttcgggggta tagctcagtt ggtagagcgc
    10141 ttgcatggca tgcaagaggt cagcggttcg attccgctta cctccaccag ataaaaaagc
    10201 acagaccgta aaaaggtatg tgctttttta ttgcctgatt gccagcaaat aaagaataaa
    10261 ccactgcctt caaaacaggc aatcgacttt aaacctatcg ccccgcctgt cctgatttta
    10321 tagtgaatta aatttaaacc ggtacagcgt tggctcgcct tgccgtacta tctgcggctt
    10381 cgtcgccttg tcctgatttt tgttaattca ctatatcagc ccgccagaca aacccgaccc
    10441 gaataatgtc ttcaggtcgg gtttatggtt tcattcccaa cttatccagc ctgacagcca
    10501 caatataatg atggcaatac caaaaacaat gcggtaatag gcaaaaggaa tataattttt
    10561 cttggaaaca aacctcagca acgctttgac cgccaccaag cctgaaacaa aggcagcaac
    10621 aaagccaatc aaaatcaaac cgacatcatg cagggtgaaa aaccggtaat gcttcaggac
    10681 atcataagcc gttgcggcaa ccatcatcgg cacagccaag aaaaacgaga attctgtcgc
    10741 agttttccgt tcgattcccc aaagcatccc gcccataatc gtactgcccg aacgggacgt
    10801 acccggaatc agtgcaaaca cttgggcaac gccgatcatc aaggcatcaa tcggacgcaa
    10861 tgcatcaaca tcggcaattt taggctctgc ccagctttgg cgtttctcca cccacaaaat
    10921 aaacaaaccg cccaaaacca gcatgaccgc cacactcaaa ggattgaaca gatgttcttt
    10981 aatctgtttg ccgaacaaca gccccatcac ggcggcaggt ataaaagcaa tggcaagatt
    11041 gaggacgaag cggttggctt tccggtcttt tcccacgcca tgcaacacat tgctgaaacg
    11101 ctgccggtat tcaaacacta ccgccaaaac tgcaccgagc tggatggcaa tttcaaaaac
    11161 cttgtgattg ctgtgaaaac caatcagatt gccgaacaca atcaaatgcc cggtgctgga
    11221 aatcggtaaa aattcggtaa aaccttctac caagcccatc atcagggctt tcaggacaat
    11281 cagaaaatcc attgcttgcg cttctttcgg atacgggagt tcggctattt ctgtacagca
    11341 gggggctgac gcttgcgttc ttccctgact ttggcaacca attctttaat cgtatgaacg
    11401 ccgccgtcaa agccattatt gaagataacg cggtattttc cgccgacaat aacggtcggc
    11461 gtgttgtcaa taccgtattg ttctgtcagt ttctgcattt ttgatgctgc tgctgcggcc
    11521 tcgggagagt cataaacgcg catcagtttt ttaccgtcaa agcctttttg agacaaagcc
    11581 cattttccgg ccaccgccct gtcttccaaa tgaaccttct gttcgtaaac tgctttaaac
    11641 acagcagggt ttgcctgata tttcaaaccc gacaaattga cggcggccgc catcctggcc
    11701 agaccgagca tttcaggctg ccagaccacg tgctccgtcc tcaaataggc atcagacggc
    11761 aatgccttgc ccaatttcaa taacaaagga tcgaaatgat ggcaatgtac gcagaaatag
    11821 ccgaaaaatt ccaaaacctc aattttgccc gactgttctt gaggaatggg tttatccaac
    11881 acaagatagt cttccctttc cgtcagggca tatgcctgcg cggacaacac tgccgacagc
    11941 agcagcggca acagatgttt gagcttcata attatttgct ttcgatagaa cggatcaggc
    12001 tggcgacttc atgttttttc aactcgtcct gcattttttt caccgcatcg gcagacatat
    12061 tgccgctttg cacccggtaa agcgttttat gtcccgcctg ataaccgacc accttggaag
    12121 atatgcccaa gattgccagt ttggcacgct gtccttccgc gctccggcgg tcggcatacg
    12181 cgcccatttg cagataatgc gttgcttccg ccttgtcggg cgttttcatt ttctgcactt
    12241 ctttggcagc ggcactgcgc gctttttcga tgctgccgct gttgaggatt tgttccgggg
    12301 tcggtttggg tgcaactttt tccttctccg cctttttctc ttcttttgaa gcttttttct
    12361 ctgtttcttt agatggtttt accgcttgtt ttttaaccgt ttcggcatct ttcttctgcg
    12421 ctttttcccc gacggtttgt tcacgctctt ccgtcagtgc tttcttgcgc actgcctgtc
    12481 cgtccgactt ttcccgctcc ggctcgtccg ccttttcctc aacctcgtcg gctttgtcgg
    12541 caacgggctg cttgtcggca gctttttccg catccgactg ctctgcctct ttcgcagcat
    12601 ccggttcgga caaggcgttt tgatcggccg gttcaggttg gatgtcttcc ttaggctggt
    12661 ttttcggttt caggatttcc gtttctgcag gctgcttcga cgaagccggg attttgaacg
    12721 cattttgacc gctctggttc agataaaaca aaataccggc aataatgacc gtcgccagta
    12781 tcaaaccgaa gaaaaaaccg gacagacctt ttccggattg ggaaaatttg ttcataaaca
    12841 taccttaatg tgtttcagac ggcattaacg ccgtttgctt acgggcggat attctaacaa
    12901 tatcgccata tttgggcaaa acctgcttcc attcccgttc ctataaagca cggcggaaac
    12961 ctatggcttt catgacgtgg cttctgccga cttcttcgtc gcctgccaaa tccgccaatg
    13021 tacgcgccac gcgcataatg cggtggaagc tgcgggcgga aagggagagt ttttccagca
    13081 ggctgcccaa tgcttcctgc gcttcttttt ggatgcgggc ggatgtgtcg agttcactga
    13141 cactcaaggc ggcattcact ttgccttgcc gtgcgtattg tttgtctcta gcggcgataa
    13201 cgcgttccaa aacggacgcg ctcctttccc ctgcttcctg ctgcatcagt tcggcggcgg
    13261 acaggctcgg gacttcgatg gtcaaatcga tgcggtcgag cagcggcccg gaaatcttgc
    13321 tgcggtaacg cgcgacgctt tcgggcgtgc agcggcaggg tttgacggga tgcccgagat
    13381 aaccgcacgg gcaggggttc atggcggcaa caagttggaa tttggcagga tagacggctt
    13441 ggcgcgccgc gcgggaaatg tggatttcgc cgttttccaa cggttcgcgc aaaacttcca
    13501 aaactttgcg gtcgaactcg ggcagctcgt ccaaaaacaa tacgccgtgg tgcgccaatg
    13561 aaatctcgcc cggacgcgga tccgaaccgc cgccgaccat agccgccgcg ctggcgctgt
    13621 gatgcggact gcggaaagga cggttgctgt cgagttgttg ttggtggttg ggcaggagcg
    13681 aacgcaatgc ccaaacttct accaattcgt cttcggtcag cggcggcagg atgccgggca
    13741 gccgttggga gagcatagac ttgcctgttc ccggtggacc catcatcaag aggctgtgtc
    13801 cgcctgcggc agcgatttcc aaagcaaggc gcgcggtgtg ctgacctttc acatcgcaca
    13861 aatcaggttg tccgccatgt tcagacggca tttgcggtac ggaacattcg gtttgcgcca
    13921 aaggttcgat accgttcaaa tgggcggcga cttcgcccaa agagcgcgcg ccgtaaacgg
    13981 taatgccgcg catcacggcg gcttgtcctg cgttttcttc aggcaaaaca aatgcacgtt
    14041 ttgcctgcat accctgccac gccatcgcca acgcgccgcg cacggggcgc aacagccccg
    14101 acagtgccaa ttcccccgca aactcgtatt ccgccagttt ttcgggcgca acctgccccg
    14161 atgcagcaag gatgccgatt gcaatcggca aatcgaaacg ccccgactct ttgggcaggt
    14221 cggcgggggc gaggttgacg gtaatttttt tggcggggaa ttcaaaaccg ctttgaataa
    14281 tggcggcacg gacgcggtcg cggctttcct ttacttccgt atcgggcagc ccgacgatgt
    14341 tgaaatgtgg caggccgttg gcaaggtggg cttccacttc gaccaacggc gcattcatac
    14401 cgctcaaggc gcggctgtaa accaaggcaa gcgacatatt tcagacggcc ttattcgccg
    14461 gcttcggttt gctgcctgat ttcggcgacg gcttcttcgg cagcggcttc agccgcttcc
    14521 aatgctgccc gttcgggatt ttgcgcggct tcgagtttcg ccaaacgcgc ttccaaagcc
    14581 gccagtttgg tacgggtttt gattaaaacc tgctgctgga tgtcgaattc ttcgcgcgta
    14641 accagatcca tacggttgaa cgcgccgccc agcatcgcct taatattttt ttccacatct
    14701 ttggcagggc tgttggcgat ggtttcgctg attttcgaac cgacttcttc aaaaagctgc
    14761 ttgccgaaca taatctgtat ccttcctgaa catatcaaat tcaatcgcct attgtataag
    14821 gaaaaatgcc gtctgaaaac gggcggcgga taatcggcaa aacataccgc gcctcctttg
    14881 cggttgctaa attacacctc aaaacacctg ccgcctgaaa acggatttca tattccgcgc
    14941 aacgcctgat tacaagacac tacaaaacaa tatactgttt taaatgattt ttccgacgcg
    15001 catcgtttca aactcggctt tttaagccat taagtgcttt gcaaacaaca gaaattgggt
    15061 tatgctgaaa cggattattt acaatttcat atagttttat tacatatctt attgtgattg
    15121 aagataattt atccgaatcc ccctttcggg tatccggatt ttccgttgta cttttattag
    15181 aaaaacattt caggcgcaag ttgcttgcaa tttcaaagcc tgtaattctg aagttttaga
    15241 cgaggaatag cacatgaatc ctatgtacat tacttttgca atctatctgg ttgcagtcct
    15301 tctcatcggg cttgccgcct atttttctac gcgcaatttc gacgattata ttttgggcgg
    15361 gcgcagccta ggcccgtttg ttaccgcgat gtcggcaggc gcgtccgata tgtccggctg
    15421 gctgctgatg ggtctgccgg gcgcgattta cctgagcggt ttgaatgagg cttggattgc
    15481 catcggcctc ttggtcggcg cgtatttcaa ctggctcttg gtggcgggcc gcctgcgcgt
    15541 gcataccgaa tacgccaaca atgcgctgac gctgccggat tatttcttcc accgcttcgg
    15601 cgcgggcgga cacttgatga aagtggtttc cgcactgatt atcctgttct tcttcacgat
    15661 ttattgcgcc tcgggcattg tggcgggcgc aaccctgttc caaagcctgt ttgaaggtat
    15721 gacttacaat caggcaatgt ggctgggcgc gggcgcgacc atcgcctata ccttcttggg
    15781 cggtttcttg gcggtcagct ggacggatac gctgcaggct tctttgatga ttttcgccct
    15841 gattttaacg ccagtgatgg tctatctggg cttgggcggc gcggaacaga tgtctgccgc
    15901 gattcaaagc gtcgccgcag gcacgggcaa agaatacggc agcctgtttg ccggtacgac
    15961 cgtcatcggc atcatttcca ccgccgcatg gggcttgggc tatttcggac agccgcacat
    16021 tttggcgcgc tttatggccg ccgaaagcgc gaaatcgctg gtatccgcac gccgcatcgg
    16081 tatgacttgg atggcgttgt gccttgcggg cgcggtagcg gtcggttatt tcggcattgc
    16141 gtatttcggt gcaaatcccg acaaagtttc ttctatgagc ggcaaccacg aacgcatctt
    16201 catcgcgctt tccaccctgc tgttcaaccc ttggatcgcc ggcatcattt tgagcgcgat
    16261 tttggcggcg gtgatgtcca ccctgtcctg ccagcttttg gtttgctcca gcgcgattac
    16321 cgaagacttc tacaaaggct ttttgcgtaa aaacgcgcaa cagtcggaac tggtatgggt
    16381 cggccgcctg atggtcttgg cgattgccgt gatttccatc ctgattgctt ccgatccgaa
    16441 cagcaaagta ttgggactgg tgtcttacgc gtgggcaggt ttcggtgcgg cattcggccc
    16501 gattgtgatt ctgtccgtat tgtggaaacg catcactgcc tacggcgcgt tgtcgggtat
    16561 ggttgcaggt gccttaaccg tggtcgtatg ggcggaatgg gtgaaaaaac cggctcaagc
    16621 ggcaggcgaa agcggcttgt tgacgatgta cgaaatcgtc ccgggcttta ttgtttgctt
    16681 gatcgttgcc gtgttggttt ccctgttcaa caaagaacct tcgcgcgaaa cccaagaacg
    16741 ctttgaaaaa gccgacgcag attaccgcgc cgcccgataa acccctttca gacggcacgg
    16801 catcccggtt cgtgccgtct gaacccgttt ccgggtcaaa cacaaataaa ccttgcctgc
    16861 atacaggcct tatttgtgtt ttacccgttt cattttcaaa gaaacggaag cacctaccct
    16921 gcccgcattt gaagccttgt atttcatctg cggatttatt acgatattac cgtattcaga
    16981 ccgcaccgat gccgtctgaa ccccccgaaa aactttggag aatccaaaaa atgtttcatt
    17041 ttgcatttcc ggcacaaact gccctgcgcc aagcgataac cgatgcctac cgccgtaatg
    17101 aaatcgaagc cgtacaggat atgttgcaac gtgcacagat gagcgacgaa gagcgcaacg
    17161 ccgcctccga gcttgcccgc cgtttggtta cccaagtccg cgccggccgc accaaagccg
    17221 gcggcgtgga tgcgctgatg cacgagtttt cactctccag cgaagaaggc atcgcgctga
    17281 tgtgtctggc agaagccctg ctgcgtatcc ccgacaacgc cacgcgcgac cgcctgattg
    17341 ccgacaagat ttcagacggc aactggaaaa gccatttgaa caacagccct tccctcttcg
    17401 tcaacgccgc cgcctggggc ctgctgatta ccggcaaact gaccgccaca aacgacagac
    17461 aaatgagttc cgcactcagc cgcctgatca gcaaaggcgg cgcaccgctc atccgccaag
    17521 gcgtaaatta cgccatgcgc ctgttgggca aacagttcgt aaccggacag accattgaag
    17581 aagccctgca aaacggcaaa gaacgcgaaa aaatgggcta ccgcttctcc ttcgatatgt
    17641 tgggcgaagc cgcctacacc caagccgatg ccgaccgcta ctaccgcgac tatgtcgaag
    17701 ccatccacgc catcggcaaa gatgcggcag gacaaggcgt ttacgaaggc aacggcattt
    17761 ccgtcaaact ctccgccata catccgcgct actcgcgcgc ccaacacggc cgtgtgatgg
    17821 gcgaactgtt gccgcgcctg aaagagctgt tccttttggg taaaaaatac gatatcggta
    17881 tcaacatcga tgccgaagaa gccaaccgtc tggagctgtc tttggatttg atggaggcac
    17941 tcgtttccga tcccgatttg gcaggctaca aaggcatcgg tttcgttgtc caagcctacc
    18001 aaaaacgttg tccgttcgtt atcgactacc tgatcgacct tgcccgccgc aacaaccaaa
    18061 aactaatgat ccgcctcgtc aaaggcgcgt attgggacag cgaaatcaaa tgggcgcaag
    18121 tggacggctt gaacggctat ccgacctaca cccgcaaagt ccacaccgac atctcctacc
    18181 tcgcctgcgc gcgcaaactg ctttccgcgc aagacgcggt atttccgcaa ttcgccaccc
    18241 acaacgccta caccttgggc gcaatctacc aaatgggcaa aggcaaagac tttgaacacc
    18301 aatgtctgca cggcatgggc gaaaccctgt acgaccaagt cgtcggcccg caaaacttag
    18361 gccgccgcgt gcgcgtgtac gccccggtcg gcacacacga aaccctgctc gcctacttgg
    18421 tgcgccgcct gttggaaaac ggcgcgaact cgtctttcgt caaccaaatc gtcgatgaaa
    18481 acatcagcat cgacacgctc atccgcagcc cgttcgacac catcgccgaa caaggcatcc
    18541 acctgcacaa cgccctgccg ctgccgcgcg atttgtacgg caaatgccgt ctgaactcgc
    18601 aaggcgtgga cttgagcaac gaaaacgtat tgcagcagct tcaagaacag atgaacaaag
    18661 ccgccgcgca agacttccac gccgcatcca tcgtcaacgg caaagcccgc gatgtcggcg
    18721 aagcgcaacc gattaaaaac cctgccgacc acgacgacat cgtcggcaca gtcagctttg
    18781 ccgatgccgc gcttgcccaa gaagcggttg gcgcagccgt tgccgcgttc cccgaatgga
    18841 gtgcgacacc tgccgccgaa cgcgccgcct gcctgcgccg tttcgccgac ctgctcgaac
    18901 agcacacccc cgcgctgatg atgcttgccg tgcgcgaagc aggcaaaacg ctgaacaacg
    18961 ccattgccga agtgcgcgaa gccgtcgatt tctgccgcta ctacgcaaac gaagccgaac
    19021 ataccctgcc tcaagacgca aaagccgtcg gcgcgattgt cgccatcagc ccgtggaact
    19081 tcccgctcgc catctttacc ggcgaagtcg tttccgcatt ggcggcaggc aacaccgtca
    19141 tcgccaaacc cgccgaacaa accagcctga ttgccggtta tgccgtttcc ctcatgcacg
    19201 aagccggcat cccgacttcc gccctgcaac tcgtcctcgg cgcaggcgac gtgggtgcgg
    19261 cattgaccaa cgatgcccgc atcggcggcg tgattttcac cggctcgacc gaagtggcgc
    19321 gcctgatcaa caaagccctt gccaaacgcg gcgacaatcc cgtcctgatt gccgaaaccg
    19381 gcggacaaaa cgccatgatt gtcgattcca ccgcacttgc cgaacaagtc tgcgccgacg
    19441 tattgaactc cgccttcgac agcgcgggac aacgctgctc cgccctgcgt atcttgtgcg
    19501 tccaagaaga cgttgccgac cgtatgctcg acatgatcaa aggcgcgatg gacgaactcg
    19561 tcgtcggcaa accgattcag ctcactaccg atgtcggccc cgtcatcgat gccgaagcac
    19621 agcaaaacct gttgaaccac atcaacaaaa tgaaaggcgt tgccaagtct taccacgaag
    19681 tcaaaaccgc tgccgatgtc gattccgaaa aatccacgtt cgtccgcccc atcctgtttg
    19741 aattgaacaa cctcaacgaa ctgcaacgcg aagtcttcgg tcccgtcctg cacgtcgtcc
    19801 gctaccgcgc cgacaaactc gacagcgtca tcgaccaaat caacagcaaa ggctacgccc
    19861 tgacccacgg cgtacacagc cgcatcgaag gcacagtacg ccacatccgc agccgcatcg
    19921 aagccggcaa cgtttacgtc aaccgcaaca tcgtcggcgc agtcgtcggc gtacagccct
    19981 tcggcggaca cggtctgtcc ggcacaggcc ccaaagcagg cggttcgttc tacctgcaaa
    20041 aactgacccg catccccgaa tgggttgccc cgaccctgag ccaaatcgga caggcagacg
    20101 aagccgcgct caaacgcctc gaagcactgg ttcacaaact accgttcaac gccgaagaga
    20161 aaaaagccgc agcggccgct ttgggacacg cccgcatccg caccctgcgc cgtgccgaaa
    20221 ccgtccttac cggcccgacc ggcgagcgca acagcatctc atggcacgcg ccaaaacgcg
    20281 tctgggtaca cggcggcagc ctcctgcaag ccttctgcgc cctaaccgaa cttgccgcct
    20341 ccggcgtaca ggcagtggtc gaacccgaca gcccgctggc ttcctacacc gccgacttgg
    20401 aaggtctgct gctggtcaac ggcaaacccg aaaccgccga catcagccac gttgccgccc
    20461 tgtcgccttt ggacagcgcg cgcaaacagg aacttgccgc ccacgacggc gcactcatcc
    20521 gcatcctccc ttcggaaaac ggactcgaca tcctgcaagt gtttgaagaa atctcttgca
    20581 gcgtcaacac cacagccgcc ggcggcaacg ccagcctgat ggcggttgcc gactgatttt
    20641 accgaaatcc ccgagcgcgg cccgtgaacc aatgccgtct gaaaaccttt cagacggcat
    20701 ttttatagtg gattaacaaa aatcagg
//
LOCUS       DAWFHY010000033        20019 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.033, whole genome shotgun sequence.
ACCESSION   DAWFHY010000033 DAWFHY010000000
VERSION     DAWFHY010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 20019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20019
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.033"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(102..1886)
                     /gene="lpdA"
                     /locus_tag="WGQ68_001042"
     CDS             complement(102..1886)
                     /gene="lpdA"
                     /locus_tag="WGQ68_001042"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEZ0714860.1"
                     /translation="MSLVELKVPDIGGHENVDIIAVEVNVGDTIAVDDTLITLETDKA
                     TMDVPAEVAGVVKEVKVKVGDKISEGGLIVVVEAEGTAAAPKAEAAAASAQEAPKAAA
                     PAPQAAQFGGSADAEYDVVVLGGGPGGYSAAFAAADKGLKVAIVERYKTLGGVCLNVG
                     CIPSKALLHNAAVIDEVRHLAANGIKYPEPELDIDMLRAYKDGVVSRLTGGLAGMAKG
                     RKVDVIQGDGQFLDSHHLEVSLTAGDAYELAAPTGEKKIVAFKNCIIAAGSRVTKLPF
                     IPEDPRIIDSSGALALKEVPGKLLIIGGGIIGLEMGTVYSTLGSRLDVVEMMDGLMQG
                     ADRDLVKVWQKQNEYRFDNIMVNTKTVAVEPKEDGVYVTFEGANAPKEPQRYDAVLVA
                     AGRAPNGKLISAEKAGVAVTDRGFIEVDKQMRTNVPHIYAIGDIVGQPMLAHKAVHEG
                     HVAAENCAGHKAYFDARVIPGVAYTSPEVAWVGETELSAKASGRKITKANFPWAASGR
                     AIANGCDNGFTKLIFDAETGRIIGGGIVGPNGGDMIGEVCLAIEMGCDAADIGKTIHP
                     HPTLGESIGMAAEVALGTCTDLPPQKKK"
     gene            complement(1963..3555)
                     /gene="aceF"
                     /locus_tag="WGQ68_001043"
     CDS             complement(1963..3555)
                     /gene="aceF"
                     /locus_tag="WGQ68_001043"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEZ0714861.1"
                     /translation="MSIVEIKVPDIGGHENVDIIAVEVKAGDTIAVDDTLITLETDKA
                     TMDVPADAAGVVKEVKVKVGDKISEGGVILTVETGAAAAEAAPAAAEAQPAPAAAPVA
                     AGGATVQVAVPDIGGHTDVDVIAVEIKVGDTVAEDDTLITLETDKATMDVPCTAAGVV
                     KAVFLKVGDKVSEGSAIIEVETVGSAAAAPAQAAQAAAPAAAPPPTAAAAPAAAPAAA
                     KIDEAAFAKAHAGPSARKLARELGVDLGQVKGTGLKGRIVGDDIKAFVKSVMQGGAAK
                     PAAAGASLGSGLDLLPWPKVDFSKFGNVEVKELSRIKKISGQNLSRNWVVIPHVTVHE
                     EADMTELEEFRKQLNKEWEREGVKLSPLAFIIKASVSALKAFPEFNASLDGDNLVLKN
                     YFNIGFAADTPNGLVVPVIKDVDQKGLKQISQELTELSKKAREGKLKPQEMQGACFTI
                     SSLGGIGGTGFTPIVNAPEVAILGVCKSQIKPVWNGKEFAPRLMCPLSLSFDHRVIDG
                     AAGMRFTVFLAKLLKDFRRITL"
     gene            complement(3705..6368)
                     /gene="aceE"
                     /locus_tag="WGQ68_001044"
     CDS             complement(3705..6368)
                     /gene="aceE"
                     /locus_tag="WGQ68_001044"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HEZ0714862.1"
                     /translation="MSTQLHDVDPIETQEWLDALSSVLEYEGGERAQYLLENLVKYCR
                     DKGVRMPHGTTTPYLNTVSVENEKGIPGDQNIEHRIRAFVRWNAAAIVLRAGKKDLEL
                     GGHIASFQSAATMYEVGFNHFWKAKGEGEEGDLVFFQGHVAPGIYARAFVEGRLTEDQ
                     LNNFRQEVDGHGLPSYPHPHLLPDFWQFPTVSMGLGPIMAIYQARFLKYLESRGLAKT
                     KGRKVWCFCGDGEMDEPESQGAIALAAREGLDNLIFVINCNLQRLDGPVRGNGKIIQE
                     LEGNFAGAGWNVVKVIWGRRWDRLLAKDKDGILRQRMEECLDGDYQTYKSKDGAYVRE
                     HFFNTPELKTLVADMTDEQLWALNRGGHDPQKVYNAYDRAANHADGKPTVILAKTIKG
                     YGMGASGEGQNVAHQAKKMDKASLKQFRDRFDIPVTDEQIESGDLPYLTFAPDTEEYK
                     YLHARRDALGGYLPQRKPTQEVLEVPELSAFDAQLKSSGEREFSTTMAFVRILSTLLK
                     DKKIGKRVVPIVPDESRTFGMEGMFRQYGIWNPKGQQYTPQDKDQLMFYKESVDGQIL
                     QEGINEPGAMADWIAAATSYANSNFAMIPFYIYYSMFGFQRIGDLAWAAGDMHARGFL
                     LGGTAGRTTLNGEGLQHEDGHSHIQADLIPNCVSYDPTFQYEVAVIVQDGLRRMYANN
                     EDVFYYITLMNENYTHPDMPEGAEQDILKGMYLLKAGGKGDKKVQLMGSGTILQEVIA
                     GAELLKADFGVEADIWSCPSFNLLHRDAIETERFNRLHPLEAEKVPFVTSQLQGHDGP
                     VIAATDYIRSYADRIRAYIPNDYHVLGTDGFGRSDSRANLRRFFEVDRYNVAVAALAA
                     LAEQGKVSKETVQQAIEKYGIKADSAPSWKR"
     gene            7062..8774
                     /locus_tag="WGQ68_001045"
     CDS             7062..8774
                     /locus_tag="WGQ68_001045"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEZ0714863.1"
                     /translation="MKASQFFISTLKEAPAEAALASHKLMIRAGLIKANASGLYTWMP
                     MGLRVLRKVENVVREEMARAGSVELLMPVVQPAELWQESGRWEFYGKELLRLKDRHDR
                     DFCMGPTCEEVIADIVRKEINSYKQLPKNFYHIQTKFRDEVRPRFGVMRAREFVMKDA
                     YSFHADYASLQTTYDAMYDSYCRIFTRLGLAFRPVAADTGSIGGTGSHEFQVLAESGE
                     DVIAYSDTSDYAANIELAPTLPLKGERAAAQAVLTKVHTPNVKTIESLVEFLNIPVEQ
                     TLKSIVVEGENEGEIVLLLLRGDHEFNDIKAEKLAGVRSPLTMASPAAIVEQFGANGG
                     SLGPVSFAGKVYADFATEKGADWVIGANEDGYHYTSFNFGRDAAEPEFVDLRNVVEGD
                     ESPDGQGRLKLARGIEVGHVFQLRDKYTKAMNVSFLDNNGKSQIMEMGCYGIGITRVV
                     AAAIEQNNDEKGIIWTKAMAPFEVVIVPMNYKKSDTVREAADKIYAELLAAGADVLLD
                     DRDERAGVLLNDSELLGIPHRIVIGDRALKEGNVEYAERRNNEAQAVAIGEIVARVTA
                     SLNA"
     gene            8837..9493
                     /locus_tag="WGQ68_001046"
     CDS             8837..9493
                     /locus_tag="WGQ68_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HEZ0714864.1"
                     /translation="MASVFLEGEAVEVGNIFCIGRNYAAHIEELKNEIPSEPVVFMKP
                     SGSILNSGGTILLPEFSRDVQFECELVLLVGKDSDGMGEGKDILGCVAGYGVGLDLTA
                     RDIQCRLKAQGLPWLKAKGFRHSACVSDFAAAGRIGNPEKMLFSLKQNGVLKQRGDTG
                     LMIYPIREILHKLAADYGLGKGDLVFTGTPSGVGAIGAGDNLALELDGLVHASFTVGC
                     "
     gene            complement(9503..9958)
                     /gene="ruvX"
                     /locus_tag="WGQ68_001047"
     CDS             complement(9503..9958)
                     /gene="ruvX"
                     /locus_tag="WGQ68_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEZ0714865.1"
                     /translation="MHKAPKGTALAFDFGETRIGVAQGDAELGLSHPLSTVTGGSNDE
                     KFAAIAKLVQEWQPRYFVVGLPVHTDGTKHEMTHLSRKFGRRLNGRFNLPVYWVDERL
                     SSVYAESLLSEAQVFGKKRKSVLDQVAAQAILHGFFEGGPAECFNGREG"
     gene            complement(9951..10499)
                     /locus_tag="WGQ68_001048"
     CDS             complement(9951..10499)
                     /locus_tag="WGQ68_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HEZ0714866.1"
                     /translation="MNLSNHFLVAMPDMEDAFFSQSVVYICKHDEDGALGIAINKPSP
                     ITMDMIFSATGKNIPMRMQHDSVMMGGPVQVDRGYVVHTPIGNWQSSIGVSDNIALTS
                     SRDVIENISREGAVDKALISIGYSSWGKGQLERELADNAWLTVPADEHILFDIPYEHR
                     YAAAFAKLGIDPLALHGKAGHA"
     gene            complement(10501..11061)
                     /locus_tag="WGQ68_001049"
     CDS             complement(10501..11061)
                     /locus_tag="WGQ68_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HEZ0714867.1"
                     /translation="MNRLLLLSAAVLLTACGSGETDKIGRASTVFNILGKNDRIEVEG
                     FDDPDVQGVACYISYAKKGGLKEMVNLEEDASDASVSCVQTASSISFDETAVRKPKEV
                     FKHGASFAFKSRQIVRYYDPKRKTFAYLVYSDKIIQGSPKNSLSAVSCFGGGIPQTDG
                     VQADTSGNLLAGACMISNPIENPDKR"
     gene            11389..13179
                     /locus_tag="WGQ68_001050"
     CDS             11389..13179
                     /locus_tag="WGQ68_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEZ0714868.1"
                     /translation="MRYKPLLLALMLVFSTPAVAAHDAAHNRSAEVKKQTKNKKEQPE
                     AAEGKKEKGKNGAVKDKKTGGKEAAKEGKESKKTAKNRKEAEKEATSRQSARKGREGD
                     KKSKAEHKKAHGKSVSGSKEKNAKTQPENKQGKKEAKGQGNPRKGGKAEKDTVSANKK
                     VRSDKNGKAVKQDKKYREEKNAKTDSDELKAAVAAATNDVENKKALLKQSEGMLLHVS
                     NSLKQLQEERIRQERIRQARGNLASVNLKQREAWDKFQKLNTELNRLKTEVAATKAQI
                     SRFVSGNYKNSQPNAVALFLKNAEPGQKNRFLRYTRYVNASNREVVKDLEKQQKALAV
                     QEQKINNELARLKKIQANVQSLLKKQGVTDAAEQTESRRQNAKIAKDARKLLEQKGNE
                     QQLNKLLSNLEKKKAEHRIQDAEAKRKLAEARLAAAEKARKEAAQQKAEARRAEMSNL
                     TAEDRNIQAPSVMGIGSADGFSRMQGRLKKPVDGVPTGLFGQNRSGGDVWKGVFYSTA
                     PATVESIAPGTVSYADELDGYGKVVVVDHGENYISIYAGLSEISVGKGYMVAAGSKIG
                     SSGSLPDGEEGLYLQIRYQGQVLNPSSWIR"
     gene            13306..14790
                     /locus_tag="WGQ68_001051"
     CDS             13306..14790
                     /locus_tag="WGQ68_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEZ0714869.1"
                     /translation="MSKPVFKKIALYTLGAISGVAVSLAVQGFAAEKDRRDNEVLPVQ
                     SIRTMAEVYGQIKANYYQDKPDADLFEGAMKGMVAGLDPHSEYMDKKGYAEIKESTSG
                     EFGGLGMEIGQEDGFVKVVSPIEDTPAERAGVKSGDFIVKIDNVSTRGMTVSEAVKKM
                     RGKPGTKITLTLSRKNADKPIVVNLTRAIIKVKSVRHHLIEPDYGYIRVSQFQERTVE
                     SVNTAAKELVKENKGKPLKGLVLDLRDDPGGLLTGAVGVSAAFLPSEAVVVSTKGRDG
                     KDRMVLKAIPEDYVYGMGGDSLAGIPAELKTIPMTVLVNSGSASASEIVAGALQDHKR
                     AVIVGTQSFGKGSVQTLIPLSNGSAVKLTTALYYTPNDRSIQAQGIVPDVEVKDKERI
                     FESREADLVGHIGNPLGGEDVNSETLAVPLEKDADKPAVKEKGKKKKDEDLSSRRIPN
                     PAKDDQLRKALDLVKSPEQWQKSLGLAAKKPVSNKDKKDKKDKK"
     gene            15119..17146
                     /gene="uvrB"
                     /locus_tag="WGQ68_001052"
     CDS             15119..17146
                     /gene="uvrB"
                     /locus_tag="WGQ68_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEZ0714870.1"
                     /translation="MEVIRYPDSPFKLHQPFQPAGDQPTAIADLLEGLSDGLAYQTLL
                     GVTGSGKTYTMANVIAQSGRPAIIMAHNKTLAAQLYAEMREFFPENAVEYFVSYYDYY
                     QPEAYVPSRDLFIEKDSAINEHIEQMRLSATKNLMTRNDVIIVATVSAIYGIGDPTEY
                     QQMVLSVKEGDTIEQRDIIATLVSMQYERGDLDFKRGSFRVRGDVIDVYPAESSENAL
                     RISLFDDEIDRLDMFDPLSGSLIQRVGRYTVFPSSHYVTPRDTVLRACESIKEELRER
                     IEFFAREQRPVEQQRIEQRTRFDLEMLYEMGFCKGIENYSRHFSGKKEGEPPPTLMDY
                     LPDNAIMFIDESHVTVTQIGGMYKGDASRKQNLVDYGFRLPSARDNRPLKFHEFEKVM
                     PQTIFVSATPAKYEEEHAGQVVEQVVRPTGLVDPQIIIRPVATQVDDLMSEINDRIQK
                     GERILVTTLTKRMAEQLTDYYSELGIKVRYLHSDIDTVERVEIIRDLRLGLFDVLVGI
                     NLLREGLDIPEVSLVAILDADKEGFLRSHRSLIQTIGRAARNVNGVAILYADKITDSM
                     KAAIDETERRREKQIKFNEEHGIVPQQIKKQVKDIIDGVYHEEDSGKGRRQGKNKVKV
                     GEIHNEEDAIKEIAKLEKAMQQAARDLQFEEASVLRDRIRGIREGLLFGSE"
     gene            17258..17545
                     /locus_tag="WGQ68_001053"
     CDS             17258..17545
                     /locus_tag="WGQ68_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714871.1"
                     /translation="MTYSFRVENINDISDIPENHLEIVRAALPSNRSNALSREPDGAQ
                     AVFVYEIDGEFRAKLFDRIKDTDSYLQATEDNYSNDPRSIEVVYVLEQQQG"
     gene            complement(17726..18442)
                     /gene="trmB"
                     /locus_tag="WGQ68_001054"
     CDS             complement(17726..18442)
                     /gene="trmB"
                     /locus_tag="WGQ68_001054"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEZ0714872.1"
                     /translation="MTDTAENQTPNDRQAGHPRSIRSFVLRQSHMTAAQQRAIDTLWD
                     SFGIDYQATPVDLDANFGSNRPKILEIGFGMGTATAEIALRLPETDFLAIDVHGPGVG
                     NLLKLINENHLENIRVMRHDAVEVVENMLQDGSLDGIHIFFPDPWHKKRHHKRRLIQA
                     PFIAKLLPKLKTGGYIHLATDWEEYAQQMLEVLSSFDSLQNTATDYAPTPDYRPETKF
                     EARGKRLGHGVWDLVFKRIR"
     gene            complement(18564..19967)
                     /locus_tag="WGQ68_001055"
     CDS             complement(18564..19967)
                     /locus_tag="WGQ68_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003674669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0714873.1"
                     /translation="MNNPDLPYRQALERLSQKQYNFTEVRRLLTEAASAGHPAAAFEL
                     AKHLMDADSPYQDREQGMEMLRIAAEQGHPYARYNLAYIQELEGAPPETLISLYRPLA
                     EEGLPEAQVRLMYLLYASRHFEEALKWAKTSAKNNNPHGQYLLAQYCRYGTPPDFETA
                     HLLYREAAAQGLAAAQWQLGLQYRFGQGTPTDTTQAINHLRAAAQQGYIPAYTPLAEL
                     ILPTAPDKAVYRFQQAAQENDPDAHAALADIYLQGKHPERNYKLALHHAEAAAAERHP
                     EGLRILGDICRYGLGMTSDKEKARHYYRQAAEAGSLTAYQKLISDSALNHPDQYGGIK
                     DSAIRRQRAEQLYQKAQALHYGLQCAPEYAAALKLYTEAAELGHSKAQTNLGSMYYFG
                     QGMTADYNEARKWFEKAAAKKDSMAFYNLACIHYSGHGVGPDKEKACRYLQEAINNGY
                     GQKSVLQELLQQWQNAV"
BASE COUNT         4718 a         4999 c         5565 g         4737 t
ORIGIN      
        1 ggagaagata ttttaatccg ctgtaaaata gaaaatgccg tctgaacatt tcagacggca
       61 tttgctcaaa taaaccttat cggctgttta ttcagtcgga tttatttttt cttttgcgga
      121 ggcaggtcgg tacaagtacc caatgccact tccgccgcca taccgatgga ttcgcctaag
      181 gtcgggtgcg ggtggatggt tttgccgatg tctgccgcgt cgcagcccat ttcgatggca
      241 aggcagactt cgccgatcat gtcgccaccg ttcggaccga caatgccgcc gccgatgatg
      301 cggccggttt cggcatcaaa aatcagcttg gtaaagccgt tgtcgcaacc gttggcaatc
      361 gcacggccgg aagccgccca cgggaagttg gctttggtga ttttgcggcc ggaggctttg
      421 gcggacagtt cggtttcgcc cacccacgcc acttcggggg aagtgtaggc aacgcccgga
      481 atcacgcgcg cgtcgaagta ggctttgtgg ccggcgcagt tttcagcagc aacgtggcct
      541 tcgtgaacgg ctttgtgtgc caacattggt tgaccgacga tgtcgccgat ggcgtagatg
      601 tgcggcacat tggtacgcat ttgtttgtcc acttcgatga agccgcgatc ggtaacggca
      661 acgcctgctt tttccgcgct gatgagtttg ccgttgggcg cgcggccggc ggcaaccaat
      721 acggcatcgt aacgttgcgg ctctttaggc gcatttgcac cttcaaaggt aacgtaaacg
      781 ccgtcttctt tcggctcgac tgcaacggtt ttggtgttga ccataatgtt gtcaaaacgg
      841 tattcgtttt gtttttgcca tactttcacc aagtcgcggt ctgcgccttg catcaggccg
      901 tccatcattt caaccacatc caaacgcgaa cccagcgtgc tgtaaaccgt acccatctcg
      961 aggccgataa tgccgccgcc gataatcagc agtttgcccg gtacttcttt cagagccaat
     1021 gcgccgctgg aatcgatgat gcgcggatct tcaggaatga aaggcagttt ggttacgcgg
     1081 ctgcctgctg caatgataca gtttttgaag gcaacgattt ttttctcgcc ggtaggggct
     1141 gccagttcgt acgcgtcgcc ggcagtcagc gatacctcca agtggtgcga atctaagaat
     1201 tggccgtcgc cttggataac atccacttta cggcctttcg ccatacctgc caaaccgccc
     1261 gtgaggcggg aaaccacgcc gtctttgtag gcgcgaagca tatcgatgtc gagttccggc
     1321 tcggggtatt tgataccgtt ggcagccaag tggcgcactt cgtcgataac ggcggcattg
     1381 tgcaacaagg ctttggaagg gatacagccg acgttcaggc aaacgccgcc caaagttttg
     1441 taacgttcga cgatggcgac tttcaagcct ttatcggcag cggcaaatgc agcggagtaa
     1501 ccgccgggac cgccacccaa tacgaccacg tcgtactcgg catcggcaga accgccaaat
     1561 tgcgcggctt gcggagcagg agcggcagct ttaggggctt cttgcgccga ggcggcagcc
     1621 gcttcggctt taggagcggc agccgtgcct tcagcttcaa cgacaacaat caaaccgcct
     1681 tcggagattt tgtcgccgac tttgactttc acttctttga ctacgcccgc aacttcggca
     1741 ggcacgtcca tcgtcgcttt gtcggtttcc aaggtaatca gggtgtcgtc aacggcgatg
     1801 gtgtcaccca cgttcacttc aaccgcgata atatctacat tttcgtgtcc gccaatgtcg
     1861 ggcactttca attcaactaa gctcatgtct catctttctg aatgatgttc aaacttcctt
     1921 ttcagacggc acagaccgct atgccgtctg aagccgatcg ggttacaggg taatgcggcg
     1981 gaagtctttc aatagcttcg ccaagaatac ggtgaagcgc ataccggccg caccgtcgat
     2041 gacgcggtgg tcgaaggaca ggctcaacgg gcacatcagg cgcggggcaa actctttgcc
     2101 gttccaaacc ggtttgattt gggatttgca cacgcccaag atggcgactt cgggagcgtt
     2161 cacaatcggc gtgaagcctg tgccgccgat accgcccaag ctggaaatgg taaagcacgc
     2221 gccttgcatt tcttgcggtt tgagcttgcc ttcgcgggct tttttagaca attcggtcag
     2281 ctcttggctg atttgtttca agcctttttg atccacgtct ttgatgacgg gaacaaccaa
     2341 gccgttcggc gtatcggctg cgaaaccgat gttgaagtag tttttcagca ccaggttgtc
     2401 gccgtccaaa gaagcgttga attcggggaa ggctttcagc gcggaaacgg aggctttgat
     2461 gatgaacgcc agaggagaca gtttcacgcc ttcgcgttcc cattctttgt tcagttgttt
     2521 gcggaattct tccagctcgg tcatatccgc ttcttcgtgt acggtaacgt gcggaatcac
     2581 aacccagttg cgagacaggt tttgaccgga gattttctta atgcgggaca attctttaac
     2641 ttcgacattg ccgaatttgg agaagtccac tttaggccac ggcagcaagt ccagaccgct
     2701 gcccaaagat gcgccggctg cggcaggttt tgccgcgcca ccctgcatta cggatttcac
     2761 aaaggctttg atgtcgtcgc ccacgatgcg gcctttcaag ccggtacctt tgacttggcc
     2821 caaatccacg cccaattcgc gcgccagttt gcgtgcggaa gggccggcgt gtgctttggc
     2881 gaaagcggcc tcgtcgattt tggcagcggc aggcgcggcg gcgggtgcgg cggcagcagt
     2941 cggaggcgga gcggcagccg gtgcggcagc ttgagcggct tgagcagggg ctgctgcggc
     3001 agagccgacg gtttctactt cgataatggc agagccttcg gatactttgt cgccgacttt
     3061 taagaatacg gctttaacga caccggcagc ggtacaaggt acgtccattg tcgctttatc
     3121 ggtttccaaa gtaatcagcg tgtcgtcttc ggcaacggtg tcgcccactt tgatttcaac
     3181 ggcgattaca tccacatcgg tatggccgcc gatatcggga acggctactt gaacggttgc
     3241 accgcctgcg gcaacgggtg cggcagcagg tgcaggttgt gcttcggcag cagcaggagc
     3301 ggcttcggca gcggcggcac cggtttcaac ggtcaaaatt acgccgcctt cggagatttt
     3361 gtcgccgact ttgactttaa cttctttcac gacaccggcc gcatcggcag gcacatccat
     3421 cgtggctttg tcggtttcca aagtaatcag ggtgtcgtca acggcgatgg tgtcgcccgc
     3481 tttaacttct acggcgatga tgtcgacgtt ttcgtgaccg ccgatatcgg ggactttgat
     3541 ttctacgata ctcatttgag ttcctttaat ctttttcagt tggatgccgt ctgaaaactg
     3601 cttcacgcgg catcggtacg gtttgtctgt tcaaaccatt cgggcattcg gttttcagac
     3661 ggcatgatgt ctgaatgggg caaacaggcc gtctgaaaca tcaatcagcg tttccagcta
     3721 ggggctgaat cggctttgat gccgtatttt tcaatggctt gttgaacagt ttctttgctg
     3781 actttgcctt gttccgccaa tgcggccaat gctgctacgg caacgttgta gcgatccact
     3841 tcaaagaagc ggcgcaggtt ggcgcgactg tcggaacggc cgaaaccgtc agtgcccaag
     3901 acgtggtagt cgttagggat gtaggcacgg atgcggtcag catagctgcg gatatagtcg
     3961 gtagcggcga taaccggacc gtcatgacct tgcagttgag aggtaacgaa aggcactttt
     4021 tcagcttcca gcggatgcag gcggttgaag cgttcggttt cgatggcgtc gcggtgcagc
     4081 aggttgaagg acgggcaaga ccagatgtct gcttccacgc cgaagtcggc tttcagcagc
     4141 tcggcaccgg caatcacttc ttgcagaatc gtaccggaac ccatcagttg gactttcttg
     4201 tcgcctttgc cgccggcttt cagcaggtac atacctttca agatgtcttg ttccgcacct
     4261 tcgggcatat ccggatgggt gtagttctcg ttcatcaggg tgatgtagta gaacacgtct
     4321 tcattattgg catacatacg gcgcagaccg tcttgtacga tgacggcgac ttcgtattgg
     4381 aaagtcgggt cataagatac gcagttcgga atcaggtcgg cctggatgtg gctgtggccg
     4441 tcttcgtgtt gcaggccttc gccgttcagc gtcgtacggc cggcagtacc gcccagcagg
     4501 aagccgcgcg cgtgcatatc gcccgccgcc caagccaagt cgccgatacg ttggaaaccg
     4561 aacatagaat agtaaatgta gaacggaatc atggcgaagt tgctgttggc gtagctggtt
     4621 gcagccgcaa tccagtcggc catcgcgccc ggttcgttaa taccttcttg caagatttga
     4681 ccgtcaacgg attctttata gaacatcagt tggtctttgt cttgaggggt atattgctga
     4741 cctttcggat tccaaatacc gtattggcgg aacatacctt ccatgccgaa agtacggctt
     4801 tcgtcgggaa cgataggtac gacgcgtttg ccgatttttt tgtctttcag caaagtggac
     4861 aggatgcgga cgaatgccat cgtggtcgag aactcgcgtt caccgctgga tttgagttgt
     4921 gcgtcgaatg ctgacagctc gggcacttcc aatacttcct gcgtcggttt gcgttgcggc
     4981 aggtagccgc ccaaagcatc gcggcgtgcg tgcaggtatt tgtattcttc cgtatcgggg
     5041 gcaaaagtca ggtaaggcag atcgccgctt tcgatttgtt cgtcggtaac cggaatgtca
     5101 aagcggtcgc ggaattgttt cagggacgct ttgtccattt ttttggcttg gtgggcaacg
     5161 ttctgacctt cgccggatgc gcccataccg taacctttaa tggttttcgc caagatgacg
     5221 gtaggtttgc cgtcggcatg gttcgctgcg cggtcgtagg cgttgtacac tttttgcggg
     5281 tcgtggccgc cgcggttcaa tgcccagagt tgctcatcgg tcatatcggc aaccaatgtt
     5341 ttcagttcgg gcgtattgaa gaagtgttcg cgcacatacg cgccgtcttt ggatttgtaa
     5401 gtttggtagt cgccgtccaa acattcttcc atacgttggc gcaggatacc gtctttgtct
     5461 ttcgccaaga ggcggtccca gcggcggccc caaatgactt tgacgacatt ccagccggcg
     5521 ccggcaaagt tgccttccaa ttcttggatg attttgccgt tgccgcgtac cggaccgtcc
     5581 aagcgttgca gattgcagtt gatgacgaaa atcaggttgt ccaagccttc gcgtgcagcc
     5641 agtgcgattg caccttgaga ttcgggttcg tccatttcgc cgtcgccgca gaaacaccat
     5701 actttacggc ctttggtttt tgccaaacca cgcgattcca agtatttcag gaaacgcgcc
     5761 tgataaatcg ccatgatggg ccccaagccc atggatacgg tcgggaactg ccaaaagtcg
     5821 ggcaagaggt gggggtgcgg ataggaaggc agaccgtgtc cgtccacttc ttggcggaag
     5881 ttgttcagct ggtcttcggt cagacggccc tcgacgaatg cgcgtgcata gatgcccggg
     5941 gcgacgtgac cttggaagaa gaccaaatcg ccttcttcgc cttcgccttt ggctttccaa
     6001 aagtggttga aaccgacttc gtacatggtg gcggcagatt ggaaagatgc gatgtgccca
     6061 cccagttcca aatctttctt gccggcgcgc aatacgatgg cggcggcgtt ccagcgcacg
     6121 aatgcgcgga tgcggtgttc gatgttttgg tcgcccggaa tgcctttttc gttttcaacc
     6181 gaaacggtat tcaaatacgg tgtggtcgtg ccgtgtggca tacgtacgcc cttgtcgcgg
     6241 cagtatttga ccaggttttc caagaggtat tgcgcgcgtt cgccgccttc atattcgagg
     6301 acggagctta acgcgtccag ccactcttgg gtttcgatag ggtcaacatc gtgtaattgg
     6361 gtggacatag tatctatcct ttatgttgag tgtttaatcg gcagggaggg tgtagccaac
     6421 ccctttattt tcgtttgtga aaaataataa gcaaaaacga aaatttctaa atcaaaccga
     6481 aaaaaataat ttaagtttaa tgttttcaaa aaattataaa agttttggaa acaagggaaa
     6541 acttgaaaaa aatgttcgat ctgctccaca cgaaatccta tcagcaatcg ggtctgctgg
     6601 caaataacca tgcctgtaaa agcaggtttt atactgaagt tggcgtttgg taaggtcaag
     6661 tctttttgat ttcgaaatat tctgtttgat tttggcgata ttttttcgga attgtggtat
     6721 atcgaatcat tatgtctctg tcggcaattt tcctattgta gaaaatctat acacaagctt
     6781 tgtggataac cgtgttgata agccacggct tgtgttaaaa aagtcttacg caacaaatgt
     6841 acgcttattt tgattaaaaa ataggcacat ttaaatctat aaaaatcatg tgcttatgtt
     6901 caatatgatg tttctggtca tgtttttgcc gtctgttatg ggatttctcc cgcgcttggg
     6961 gcagggggtg aattttggag gttggtgcaa aacctgcttt tttcgcgtta caatctgcac
     7021 ccgatttact tttgaatttg cctgtttgga aaaatacttt tatgaaagcc agccaattct
     7081 ttatctctac tttaaaagaa gcccctgccg aagccgcgct tgccagccac aagctgatga
     7141 ttcgcgccgg tctgattaaa gccaacgcgt ccggccttta tacttggatg ccgatggggc
     7201 tgcgcgtgtt acgcaaagtc gaaaacgtcg tgcgcgagga aatggcgcgc gcgggcagcg
     7261 tggagctgct gatgccggtg gtgcagcctg ccgagctgtg gcaggaatcc ggccgctggg
     7321 aattttacgg taaagaactg ctgcgcctga aagaccgcca cgaccgcgat ttctgcatgg
     7381 gcccgacctg cgaggaagtc atcgccgaca tcgtgcgcaa agaaatcaac agctacaaac
     7441 agctgccgaa aaacttttac cacatccaaa ccaaattccg cgacgaagtg cgcccgcgtt
     7501 tcggcgtgat gcgcgcgcgc gagttcgtca tgaaagacgc ttattccttc catgccgact
     7561 acgcctcgct tcagacgacc tatgatgcca tgtatgactc ttactgccgc atcttcaccc
     7621 gtctgggctt ggcattccgc cccgtcgccg cagacaccgg cagcatcggc ggtacgggtt
     7681 cgcacgagtt tcaagtgttg gcggaaagcg gcgaagatgt tattgcgtac agcgacactt
     7741 ccgattacgc cgccaatatc gagttggcac cgaccttgcc gcttaaaggc gaacgcgccg
     7801 ctgctcaggc tgtgttgacc aaagtacata caccaaacgt caaaaccatt gagtctttgg
     7861 ttgaattcct gaatattccg gttgaacaaa ccctcaaatc catcgtggtc gaaggcgaaa
     7921 acgaaggcga aatcgtctta ttactgttgc gcggcgacca tgagttcaac gacatcaagg
     7981 cagaaaaact cgcaggcgtg agatcgccat tgacgatggc aagtcctgcc gcgattgtcg
     8041 aacagttcgg cgcaaacggc ggctcgctgg gtccggtcag ctttgcaggt aaagtttatg
     8101 ccgattttgc tactgaaaaa ggagcggact gggtcatcgg tgcgaacgaa gacggctacc
     8161 actacaccag cttcaacttc gggcgcgatg ccgccgagcc tgaattcgtc gatttgcgta
     8221 atgtggtcga aggcgacgaa agccccgacg ggcaaggccg tctgaaactc gcgcgcggca
     8281 ttgaagtcgg acacgtcttc cagttgcgcg acaaatatac taaagccatg aacgtcagct
     8341 tcctcgacaa caacggcaaa tcgcaaatta tggaaatggg ctgctacggc atcggcatca
     8401 cccgcgtcgt tgccgccgcc atcgagcaga ataacgacga aaaaggcatc atctggacca
     8461 aagcaatggc gccgtttgaa gtcgttatcg tgccgatgaa ctacaaaaaa tcagacaccg
     8521 tacgcgaagc cgccgacaaa atctatgccg aattgctggc ggcaggcgcg gatgtgctgc
     8581 tggatgaccg cgacgaacgc gcgggcgtat tgctgaacga ctccgagctg ttgggcatcc
     8641 cgcaccgcat cgtcatcggc gaccgcgcct tgaaagaagg caatgtcgaa tacgccgaac
     8701 gccgcaacaa cgaagcgcag gcagttgcaa tcggagaaat tgttgcgcgt gtaacagctt
     8761 cattaaatgc gtaagcttgg tcaaatgctg tctgaaaata atttcagacg gcatttttat
     8821 ttggggagaa agtgaagtgg cttcagtatt tttagaagga gaagcagtcg aggtcggtaa
     8881 tattttctgt atcggcagga actatgccgc ccatatcgaa gagttaaaaa acgaaatacc
     8941 ttccgaacct gtggtgttta tgaagccgtc aggcagcatt ttgaatagcg gcggtacaat
     9001 cttgctgccc gagtttagta gggatgtgca gtttgagtgc gaactcgtgt tgctggtcgg
     9061 taaggattca gacggcatgg gcgagggtaa agatattttg ggatgtgtgg cggggtacgg
     9121 cgtgggtttg gatttgaccg cgcgagacat ccaatgccgt ctgaaggcgc aggggctgcc
     9181 ctggctgaag gcaaaagggt tcaggcattc cgcgtgcgtg tcggattttg cggcggcagg
     9241 cagaatcggc aatcccgaga agatgttgtt ttcattaaaa caaaacggcg tattgaaaca
     9301 gcgcggggat accggtttga tgatttaccc gattcgggaa attttacaca aactggcggc
     9361 agattacggt ttggggaaag gggatttggt gtttaccgga acgccctccg gcgtgggcgc
     9421 aatcggtgcg ggggacaatt tggcattgga gctggacggc ttggtgcacg cctctttcac
     9481 ggtagggtgt taaccgcgcc gcttaaccct cacgcccgtt gaaacattcc gccggaccgc
     9541 cctcgaaaaa accgtgcaag atggcttgcg ccgccacttg gtcgagcacc gatttgcgtt
     9601 ttttgccgaa gacctgtgct tccgaaagca ggctttcggc atagacggac gacagccgtt
     9661 cgtccaccca atagacgggg agattgaacc tgccgttcag cctgcgtccg aacttgcgcg
     9721 acaggtgcgt catttcatgt ttcgtgccgt cggtatgcac gggcagtccg acgacaaaat
     9781 aacgcggctg ccattcttga accagcttgg cgattgccgc gaacttttca tcgttgctgc
     9841 cgccggtaac ggtgctcaaa ggatgggata gccctaattc cgcgtctcct tgtgccacgc
     9901 cgatacgcgt ttcgccgaag tcgaatgcca atgctgtacc ttttggggct ttatgcatgt
     9961 cccgcctttc cgtgcaaagc cagggggtcg atgccgagtt tggcgaatgc ggcggcgtaa
    10021 cggtgttcgt aggggatgtc gaacaggatg tgttcgtcgg cgggaacagt cagccacgca
    10081 ttgtcggcaa gttcgcgttc gagctggcct tttccccagc ttgaatagcc tatgctgatc
    10141 aaggctttgt caaccgcacc ttcgcgtgaa atattttcaa tcacgtctcg ggaagaagtt
    10201 agcgcgatat tgtctgaaac gccgatactg ctttgccagt tgccgatcgg ggtatgcacg
    10261 acataacctc gatcgacttg caccggcccg cccatcatca cgctgtcgtg ctgcatccgc
    10321 atggggatgt ttttgccggt ggcggaaaaa atcatgtcca tcgtaatcgg agagggtttg
    10381 ttgatggcga tgccgagtgc gccgtcttca tcgtgtttgc agatatagac gaccgattgt
    10441 gaaaaaaacg cgtcttccat atcgggcatg gcgacgagaa agtggttgga gaggttcata
    10501 tcagcgtttg tcgggatttt ctatcgggtt ggaaatcatg caggcgccgg caagcaggtt
    10561 gccggaagta tcggcttgca ccccatcggt ttgcggtatg ccgccgccga aacaggaaac
    10621 cgcgcttaag gaatttttcg gcgagccttg gatgatttta tcgctgtaca ccaaataggc
    10681 gaaggttttg cgtttggggt cgtaataacg gacaatctgc cggctcttga acgcgaagct
    10741 cgcaccgtgt ttgaaaactt ctttcggttt gcgcacggcg gtttcgtcaa aagaaatcga
    10801 agatgccgtc tgaacgcacg aaaccgatgc gtcggacgcg tcctcttcca aattgaccat
    10861 ttccttcaag ccgccttttt ttgcatacga aatataacag gcaacccctt gaacgtcggg
    10921 atcgtcgaat ccttccactt cgatacggtc gtttttgccc agtatgttga aaacggtact
    10981 tgcccgtccg attttatcgg tttcgccgct gccgcaggca gtcagcagga cggcggcaga
    11041 cagcagtagc agtctgttca tcaaaatttc cttatcggtt gaatccccgt tttccgtgaa
    11101 ggcggcaggg cttgctttgt ttggaggggc gcacgttccc aaaatcgggc aacatacggt
    11161 atgcggctta atgtgctacc ctgtgtgtcg gaacacaata tccgttgcgg cattgaaaat
    11221 atgacgcaca atataaggct gtttgagccg attgcaagta tccgcgcaaa gaaggcgcaa
    11281 gattgggcaa acggcatttt cagacggcat aactgacagt ataatccaaa cgtccggctg
    11341 ctttcgtgca gccttcagta ttatccaact ttttccgaag gtcgaaccat gcgctacaaa
    11401 ccccttctgc ttgccctgat gctcgttttt tccacgcccg ccgttgccgc ccacgacgcg
    11461 gcacacaacc gttccgccga agtgaaaaaa cagacgaaga acaaaaaaga acagcccgaa
    11521 gcggcggaag gcaaaaaaga aaaaggcaaa aatggcgcag tgaaagataa aaaaacaggc
    11581 ggcaaagagg cggcaaaaga gggcaaagag tccaaaaaaa ccgccaaaaa ccgcaaagaa
    11641 gcagagaagg aggcgacatc caggcagtct gcgcgcaaag gacgcgaagg ggataagaaa
    11701 tcgaaggcgg aacacaaaaa ggcacatggc aagtccgtgt ccggatccaa agaaaaaaac
    11761 gcaaaaacac agcctgaaaa caaacaaggc aaaaaagagg caaaaggaca gggcaatccg
    11821 cgcaagggcg gcaaggcgga aaaagacact gtttctgcaa ataaaaaagt ccgttccgac
    11881 aagaacggca aagcagtgaa acaggacaaa aaatacaggg aagagaaaaa tgccaaaacc
    11941 gattccgacg aattgaaagc cgccgttgcc gctgccacca atgatgtcga aaacaaaaaa
    12001 gccctgctca aacaaagcga aggaatgctg cttcatgtca gcaattccct caaacagctt
    12061 caggaagagc gtatccgcca agagcgtatc cgtcaggcgc gcggcaacct tgcttccgtc
    12121 aacctcaaac agcgcgaggc ttgggacaag ttccaaaaac tcaataccga gctgaaccgt
    12181 ttgaaaacgg aagtcgccgc tacgaaagcg cagatttccc gtttcgtatc ggggaactat
    12241 aaaaacagcc agccgaatgc ggttgccctg ttcctgaaaa acgccgaacc gggtcagaaa
    12301 aaccgctttt tgcgttatac gcgttatgta aacgcctcca atcgggaagt tgtcaaggat
    12361 ttggaaaaac agcagaaggc tttggcggta caagagcaga aaatcaacaa tgagcttgcc
    12421 cgtttgaaga aaattcaggc aaacgtgcaa tctctgctga aaaaacaggg tgtaaccgat
    12481 gcggcggaac agacggaaag ccgcagacag aatgccaaaa tcgccaaaga tgcccgaaaa
    12541 ctgctggaac agaaagggaa cgagcagcag ctgaacaagc tcttgagcaa tttggagaag
    12601 aaaaaggccg aacaccgcat tcaggatgcg gaagcaaaaa gaaaattggc tgaagccaga
    12661 ctggcggcag ccgaaaaagc cagaaaagaa gcggcgcagc agaaggctga agcacgacgt
    12721 gcggaaatgt ccaacctgac cgccgaagac aggaacatcc aagcgccttc ggttatgggt
    12781 atcggcagtg ccgacggttt cagccgcatg caaggacgtt tgaaaaaacc ggttgacggt
    12841 gtgccgaccg gacttttcgg gcagaaccgg agcggcggcg atgtttggaa aggcgtgttc
    12901 tattccactg caccggcaac ggttgaaagc attgcgccgg gaacggtaag ctatgcggac
    12961 gagttggacg gctacggcaa agtggtcgtg gtcgatcacg gcgagaacta catcagcatc
    13021 tatgccggtt tgagcgaaat ttccgtcggc aagggttata tggtcgcggc aggaagcaaa
    13081 atcggctcga gcgggtcgct gccggacggg gaagaggggc tttacctgca aatacgttat
    13141 caaggtcagg tattgaaccc ttcgagctgg atacgttgat cggattgccg aaacggtagg
    13201 ctttatgcaa atcgtgccga tatggtgcgc tgttccgtga agtttgccgt ttgccgtacc
    13261 tgtattttcc ggtttttgga aatcaagaga gaaagttaaa gaagaatgtc gaaacctgtt
    13321 tttaagaaaa tcgcacttta tactttgggt gcaatcagcg gcgtggccgt cagtctggcg
    13381 gtgcagggtt ttgccgccga gaaggacagg cgggataacg aagtcctgcc ggtgcaatcc
    13441 atccgcacaa tggcggaggt ttacggtcaa atcaaggcaa actactatca ggacaaaccc
    13501 gatgccgatt tgtttgaagg tgcgatgaag ggtatggtgg ccggtttgga tccgcattcc
    13561 gaatatatgg ataaaaaagg ttatgccgag ataaaggagt ccaccagcgg cgaatttggc
    13621 ggcttgggga tggaaatcgg gcaggaagac ggatttgtca aagtggtttc gccgattgag
    13681 gacacgcctg cggaacgggc gggggtgaaa agcggcgatt tcattgtgaa aatcgataat
    13741 gtttcgacac gcggcatgac ggtcagcgaa gcggtgaaga aaatgcgggg caagccgggt
    13801 acgaagatta ctttgacgct gtcgcgcaaa aatgccgaca agccgatagt cgtcaacctg
    13861 acccgtgcca ttattaaagt gaaaagcgtc cgccatcacc tgatcgaacc cgattacggc
    13921 tatatccgcg tgtcgcagtt ccaagagcgg acggtcgaaa gcgtcaatac cgccgcaaaa
    13981 gagctggtaa aggaaaataa aggaaaaccg ctcaaggggc tggtgttgga tttgcgcgac
    14041 gaccccggcg ggcttttgac tggcgcggtc ggcgtgtcgg cggcatttct gccgtctgaa
    14101 gcagtcgtcg tcagcaccaa gggacgcgac ggcaaagacc gcatggtact gaaagccatt
    14161 cctgaagatt atgtgtacgg gatgggcggc gattcgttgg cgggcattcc tgccgagttg
    14221 aaaaccatac cgatgacggt attggtcaat tccggttcgg cttccgcgtc ggagattgtc
    14281 gcaggtgcat tgcaggatca taaacgcgcg gtcatcgtcg gtacgcagag cttcggcaaa
    14341 ggttcggttc agactttgat tcctttgtcc aacggcagcg cggtcaagct gacaacggca
    14401 ctgtattata cgccgaacga ccgttctatt caggcgcagg ggattgttcc cgatgtcgaa
    14461 gtaaaagata aggaacgcat ttttgaaagc cgcgaggcgg atttggtcgg acacatcggc
    14521 aatcctttgg gcggcgagga tgtgaacagt gaaacccttg ccgtgccgct tgaaaaagat
    14581 gcggataagc ccgctgtaaa agaaaaaggt aaaaagaaaa aggacgagga tttgtcttca
    14641 aggcggattc ccaaccctgc caaagacgac cagttgcgga aagctttgga tttggtcaag
    14701 tcgcccgagc agtggcagaa gtctttgggg ctggcggcga aaaagccggt ttcaaataaa
    14761 gataagaaag ataaaaaaga taagaagtag gtttttacga tgccgtctga aaggatttca
    14821 gacggcatat tatgtttgaa aggacaggtt atgagcgatt ttttaccgga atttgaaccg
    14881 tgcggcattt atagtgggtt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
    14941 aaatagtacg gcaaggcgag gcaacgccgt actggttttt gttaatccac tatacttggg
    15001 tagcgattgg gtttcagacg gcatacattc aggatttcac cgccttatgc cgtctgaaag
    15061 acgtttccga cccatcccta ctacacgctt tgtccgcaaa cgcatcacag gcaagattat
    15121 ggaagtcatt cgataccccg attccccctt caaactccac caacccttcc agcccgcagg
    15181 cgaccagcct accgctattg ccgacttgct cgaagggctt tcagacggcc ttgcctacca
    15241 aaccctgctc ggcgtaaccg gttcgggcaa aacctacacc atggcgaacg tcatcgccca
    15301 aagcggccga cccgccatca tcatggcgca caacaaaacc cttgccgccc agctttatgc
    15361 cgaaatgcgc gagtttttcc ctgaaaacgc ggtggaatat ttcgtctcct actacgacta
    15421 ttaccaaccc gaagcctatg tgccaagccg cgatttgttc atcgaaaaag acagtgcgat
    15481 caacgaacac atcgagcaga tgcgcctttc cgccaccaaa aacctaatga cgcgcaacga
    15541 cgtgattatc gtcgccaccg tgtccgccat ttacggtatc ggcgacccga ccgagtatca
    15601 acaaatggtg ttgtccgtca aagaaggcga taccatcgag cagcgcgaca tcatcgccac
    15661 gctcgtttcc atgcaatacg aacgcggcga tttggacttc aaacgcggca gcttccgcgt
    15721 gcgcggcgac gtgattgacg tgtaccccgc cgaaagctcc gaaaacgcct tacgcatcag
    15781 cctgtttgat gacgaaatcg accgcctcga tatgttcgac ccgctttcag gcagccttat
    15841 ccagcgcgtc ggccgctaca ccgtcttccc gtccagccac tacgttaccc cgcgcgacac
    15901 cgtcttgcgc gcctgcgagt ccatcaaaga agaattacgc gaacgcatcg aatttttcgc
    15961 ccgcgaacaa cgccccgtcg aacaacaacg catcgaacag cgcacccgtt tcgacctcga
    16021 aatgctctac gaaatgggct tctgcaaagg aatcgaaaac tactcccgcc acttttccgg
    16081 caaaaaagaa ggcgaaccgc cgcctacgct gatggactac ctgcccgaca acgccatcat
    16141 gttcatcgac gaaagccacg ttaccgtaac ccaaatcggc ggcatgtaca aaggcgacgc
    16201 atcgcgcaag caaaacctcg tcgactacgg cttccgcctg ccttcagccc gcgacaaccg
    16261 cccgctcaaa ttccacgaat ttgaaaaagt catgccgcaa accatcttcg tttccgctac
    16321 ccccgccaaa tacgaagaag aacacgccgg acaagtggtc gaacaagtcg tccgtcccac
    16381 agggctggtc gacccccaaa tcatcatccg ccccgtcgcc acccaagttg atgacttaat
    16441 gagcgaaatc aacgaccgca tccaaaaagg cgaacgcatc ctcgttacca ccctcaccaa
    16501 acgtatggcg gagcaactca ccgactatta cagtgaactc ggcatcaaag tgcgctacct
    16561 gcacagcgac atcgataccg tcgagcgcgt tgaaatcatt agagatttgc ggctcggcct
    16621 gtttgacgta ctcgtcggca tcaacctctt gcgcgaaggc ctcgacatcc ccgaagtctc
    16681 ccttgttgcc atcctcgacg ccgacaaaga aggcttcctg cgctcccacc gcagcctgat
    16741 tcaaaccata ggccgcgccg cgcgcaacgt gaacggcgtc gcgattctgt acgccgacaa
    16801 aatcaccgac tctatgaaag ccgccatcga cgaaaccgaa cgccgccgtg aaaaacaaat
    16861 taaattcaac gaagaacacg gcatcgtgcc gcaacagatt aaaaaacagg tcaaagacat
    16921 cattgacggc gtgtaccacg aagaagacag cggcaaaggc cgtagacaag gcaaaaacaa
    16981 agttaaagtc ggcgaaattc acaacgaaga agacgcgatt aaagaaatcg ccaaattgga
    17041 aaaagccatg caacaggcag ctagggattt gcagtttgaa gaagcgtcgg tgttaaggga
    17101 taggattcgg gggattaggg aagggttgtt atttgggagt gagtaataaa agcacttgaa
    17161 agtttagttg atgaccacaa tgttcttaaa agatttggag aaacaaaaga tgtggagaaa
    17221 catcgtaact tgatcaatta cattctgagg actgaaaatg acatattctt ttcgtgtaga
    17281 aaacataaat gatatatctg atattcctga aaatcatttg gaaatagtta gagctgctct
    17341 gcctagtaat agaagtaatg ccttatctcg tgagccagat ggggcacaag ctgtttttgt
    17401 ttatgaaatt gatggtgaat ttagagctaa gttatttgac cgcataaaag atacagacag
    17461 ttatttacaa gcaacagagg ataattactc taatgaccct cgcagtatag aagtagtata
    17521 cgttttggag caacagcaag ggtagcctga gggtcgcaca aactctcaag ctgacgtgca
    17581 gtatgtgtga tacgcacgca tgcgtttttt cgttcaaacc attgttttaa accttgtatc
    17641 gaaacatcac tcgctgcttt atcttgaaaa aatgccgtct gtaagcaaac atgcttcaga
    17701 cggcattttt tcaaacagcg gtttgttatc tgatccgttt gaataccaag tcccaaacgc
    17761 cgtgtccgag acgtttgccg cgcgcttcga atttggtttc ggggcggtag tccggggtag
    17821 gggcgtagtc tgtcgccgta ttttgcaggc tgtcgaagct actgaggact tcaagcatct
    17881 gctgtgcata ttcttcccag tctgtcgcca ggtggatata gccgccggtt ttgagtttgg
    17941 gcagtagttt ggcgatgaag ggggcttgta tcagacggcg tttgtggtgg cgttttttgt
    18001 gccacgggtc ggggaagaat atgtggatgc cgtcgagcga gccgtcttgc agcatatttt
    18061 cgacaacttc tacggcatcg tgccgcatca cgcggatgtt ttctaaatgg ttttcgttga
    18121 tgagtttgag caggttgcct acgccgggac cgtgtacgtc gatggcgagg aagtcggttt
    18181 cgggcaggcg gagggcgatt tctgcggttg ctgtccccat accgaagcct atttcgagga
    18241 ttttggggcg gttgcttccg aaattggcat caagatcgac cggtgttgct tggtaatcga
    18301 tgccgaagct gtcccataag gtatcgatgg cgcgttgctg cgcggcggtc atatggcttt
    18361 ggcggaggac gaaactgcgg atgctgcggg gatgtccggc ttgacggtcg tttggtgttt
    18421 ggttttcggc ggtgtctgtc ataatgcttc cgtgcgcttg tgcaatcagg atgccgtctg
    18481 aatgtgaaag gcctgttcgg acggcgggaa gggaaagggg cgattatacc tgtttcgttt
    18541 cggcagggta ggtgtaacgc tgttcagacg gcattttgcc attgttgcag cagttcttgc
    18601 aggacgcttt tttgcccgta tccgttgttt atggcttctt gcaggtagcg gcaggctttt
    18661 tctttgtccg gcccgacacc gtgtccgctg taatggatgc aggcgaggtt gtagaacgcc
    18721 atactgtctt tttttgcggc ggctttttca aaccatttgc gtgcttcatt gtagtcggcg
    18781 gtcataccct gtccgaagta atacatgctg cccagattgg tttgggcttt gctgtgtccg
    18841 agttctgcgg cttctgtgta gagtttgagc gcggctgcgt attcgggcgc gcattgtaat
    18901 ccgtaatgca gggcttgggc tttttgataa agctgttctg ccctttgccg cctaatggcg
    18961 gaatctttaa tgccgccgta ttggtcagga tggtttaacg cgctgtcgga tatgagtttc
    19021 tgataggcgg taaggctgcc ggcttcggct gcctgccggt aataatgccg ggctttttct
    19081 ttatcggaag tcatgcccag tccgtagcgg cagatgtcgc ccagtatccg caaaccttcg
    19141 ggatggcgtt cggcggcggc tgcttcggcg tgatgcaggg caagtttgta gtttctttcc
    19201 ggatgcttgc cttgcaggta gatgtcggca agtgcggcat gggcatcggg gtcattttcc
    19261 tgtgcggcct gttgaaaccg gtaaacggct ttatcgggag ccgtaggtag gatgagctcg
    19321 gcaagcgggg tgtaggcggg aatgtatcct tgttgtgcgg cggcgcgcaa atgattgata
    19381 gcttgggtcg tgtcggttgg tgtaccttgt ccgaaacggt attgaaggcc gagctgccat
    19441 tgtgccgccg ccaagccttg tgctgcagcc tcgcggtaga gaaggtgtgc cgtttcaaaa
    19501 tccggcggcg tgccgtaccg gcagtattgg gcaagcaggt attgcccgtg ggggttgttg
    19561 ttttttgcgc ttgtttttgc ccatttcagg gcttcttcaa aatgtcggga cgcatacagc
    19621 aggtacatca ggcggacttg tgcttcaggc agcccttcct ctgctaatgg tctgtaaagc
    19681 gatatcaggg tttccggggg tgcgccttcc aattcttgga tatatgccag attgtaacgc
    19741 gcgtagggat gtccctgttc ggcggcgatg cggagcattt ccataccttg ttcgcggtct
    19801 tggtaggggc tgtccgcgtc catcaggtgt tttgccaact cgaatgcggc ggcgggatga
    19861 cctgccgagg ccgcttctgt cagcagtcgg cggacttcgg taaagttata ttgtttttga
    19921 gacaggcgtt ctaaggcctg ccgatagggt aagtcgggat tgttcatatc agatcgtttt
    19981 ccgttttgtc gaatcatcgt tattgtgtta tgaagttag
//
LOCUS       DAWFHY010000034        19941 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.034, whole genome shotgun sequence.
ACCESSION   DAWFHY010000034 DAWFHY010000000
VERSION     DAWFHY010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.034"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            316..1629
                     /locus_tag="WGQ68_001056"
     CDS             316..1629
                     /locus_tag="WGQ68_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HEZ0714874.1"
                     /translation="MLTFIGLLIIGVIVWLLLTEKVSPIIALILVPLFGALLAGFDVS
                     QLKEFYSGGTKSVMQIVIMFMFSILFFGIMNDVGLFRPMIGGLIKLTRGNIVAVSVGT
                     VLVSVVAQLDGAGATTFLLVVPALLPLYKRLHMNPYLLFLLLTSSAGLINLLPWGGPT
                     GRVASVLGADVGELYKPLLTVQIIGVVFILALSLLLGVREKRRIVRELGALPAVADLI
                     KPVPLSEEEQKLARPKLFWWNVLLFLAAMSLLFSGIFPPGYVFMLAATAALLLNYRSP
                     QEQMERIYAHAGGAVMMASIILAAGTFLGILKGAGMLDAISKDIVHILPDALLPYLHI
                     AIGVLGIPLELVLSTDAYYFGLFPIVEQITSQAGVAPEAAGYAMLIGSIVGTFVTPLS
                     PALWMGLGLAKLSMGKHIRYSFFWAWGLSLAILASSIAAGIVPLP"
     gene            1852..2009
                     /locus_tag="WGQ68_001057"
                     /pseudo
     CDS             1852..2009
                     /locus_tag="WGQ68_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692565.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
     gene            2022..3272
                     /locus_tag="WGQ68_001058"
     CDS             2022..3272
                     /locus_tag="WGQ68_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0714875.1"
                     /translation="MFVPLAMLAGMFSYYETFHETEALQDDLLRQAALYVAPDSKPET
                     LPEGDGDTRILVQMPQQEDPVVSLPAHLADGLHTLQADGDDDYYRVYIRTTEQGRIAV
                     MQENEYREDLAADAARQSVLPLLAALPLMILLTVWITHKAMRPVRKLSQSLEQRRIND
                     LSALSVDNIPSEIRGFVTAINLLLKRADEDIRHRQRFVADAAHELRTPMTALSLQAER
                     LNNMSLPPDAARQPAVLQQSIRRNKHLLEQLLALARSQSDETPLTKTTFGLQSRFRQV
                     LQELMPLALEKRQDIGVAVRGDVEVSADETEIYTLIKTFADNAVRYTPPEGRIDLGFT
                     DEGKYLAVWVEDNGNGIPESERARVLDPFYRILGTEQQGTGLGLSIADTLAKKYGGHL
                     ELTDSRRFGHGLLIRALLDKETLK"
     gene            complement(3307..4800)
                     /gene="rng"
                     /locus_tag="WGQ68_001059"
     CDS             complement(3307..4800)
                     /gene="rng"
                     /locus_tag="WGQ68_001059"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HEZ0714876.1"
                     /translation="MLSGLPIPKDIARPPETILVNITPQETRVAVLEENNICELHIER
                     NSEHSLVGNIYLGVVRRVLPGMQSAFIDIGLERAAFLHIVDVLEQRRNPEETQRIEHM
                     LFEGQSVLVQVIKDPINTKGARLSTQISLAGRFLVHLPQEDHIGVSQRIEDDAERSSL
                     RERLDKLLPENACRGYIIRTNAENATDEQLQSDIDYLTKVWEHIQEQAKIRPPETLLY
                     QDLPLSLRVLRDMVGCDTQKILVDSTVNHGRMTRFAEQYVHGALGRIELFKGERPLFE
                     THNVEQEISRALQPRVNLNFGSYLIIESTEAMTTIDVNTGGFVGARNFDETIFRTNLE
                     ACHTIARELRLRNLGGIIIIDFIDMAQESHREAVLQELAKALAFDRTRVTLHGFTSLG
                     LVELTRKRSRENLNQVLCEPCPSCQGRGRLKTPQTVCYEIQREIVREARRYDAESFRI
                     LAAPNVIDLFLDEESQSLAMLIDFIGKPISLAVETAYTQEQYDIVLM"
     gene            5014..5271
                     /gene="grxC"
                     /locus_tag="WGQ68_001060"
     CDS             5014..5271
                     /gene="grxC"
                     /locus_tag="WGQ68_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEZ0714877.1"
                     /translation="MQTVTMYTGPFCPYCTMAKRLLHAAGVGHIDEIRVDASPEAFAE
                     MQQLSGQRSVPQIFIGETHVGGFTDLYRLQQEGGLDGLLNP"
     gene            5292..5735
                     /gene="secB"
                     /locus_tag="WGQ68_001061"
     CDS             5292..5735
                     /gene="secB"
                     /locus_tag="WGQ68_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEZ0714878.1"
                     /translation="MSEELQPVFSIERLYVKDLSLEVPHAPQIFLEQGEPEVDMRVST
                     GSQKLEDGYYNVDVTVTVTAKLDNERTMFLNEVTQSGIFRLENIPEEDADLLLGVACP
                     NILFPYAREAVSGTVTRAGFPPVLLAPINFEAIYQQQQEAEAAGA"
     gene            5821..7863
                     /gene="recG"
                     /locus_tag="WGQ68_001062"
     CDS             5821..7863
                     /gene="recG"
                     /locus_tag="WGQ68_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEZ0714879.1"
                     /translation="MMSPETRKQLKITDVSAKKLDKLNLHTAWDLVLHLPLRYEDETH
                     IMPIKDAPIGVPCQVEGEVIHQEVTFKPRKQLIVQIADGSGSVLFLRFIHFYASHQKQ
                     TAVGKRIRAVGEIKHGFYGDEMIHPKIRDAEGGGLAESLTPVYPTVNGLNQPTLRRII
                     QTALDVTPLHDTLPDALLCRLKLPHLAESLRLLHSPPPSFTIHQLSDGTLPAWQRLKF
                     DELLAQQLSMRLARQKRIGGTAAALGGDGTLTQALRQALPFALTDAQEKVVSEICRDM
                     AQTYPMHRLLQGDVGSGKTIVAALSALTAIESGAQVAVMAPTEILAEQHFIKFKQWLE
                     PLGIEVVRLFGSLRKKAKDEAKAKLADGSVKIAVGTHALFSDGVAFHNLGLTIVDEQH
                     RFGVAQRLALKNKGREVHQLMMSATPIPRTLAMSFFADLDVSVIDELPPGRTPIKTRL
                     VNNVRRAEVEGFVLGTCRKGRQAYWVCPLIEESETLQLQTAAETLARLQTALPELNIG
                     LVHGRMKAAEKAEVMARFSSGGLNVLVATTVIEVGVDVPNAALMVIEHAERMGLAQLH
                     QLRGRVGRGAAESVCVLLFAEPLGELAKARLKVIYEHTDGFEIARQDLNIRGPGEFLG
                     ARQSGVPMLRFAKLEEDLHLLEQVREIAPMLIEQNPEIVEAHLARWLSGREGYLGV"
     gene            complement(8688..9731)
                     /locus_tag="WGQ68_001063"
     CDS             complement(8688..9731)
                     /locus_tag="WGQ68_001063"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEZ0714880.1"
                     /translation="MSKKIKVGIVGATGYTGVELLRLLAAHPDVEVAAVTSRSEAGTA
                     VADYFPSLRGVYGLAFQTPDEAGLEQCDIVFFATPNGIAMKDAPRLIEQGVRVIDLSA
                     DFRIRDIPTWEHWYGMTHAAPDLVSQAVYGLSELNREAVAQARLVANPGCYPTCVSLP
                     LVPLLRQCRLKPGMPLIADCKSGVSGAGRKGNVGSLLCEAGDNFKAYGIAGHRHLPEI
                     RQTIAGLQDGIAEGFVFTPHLAPMIRGMHATVYLHLSDGSNPETVLRDYYRDSLFVDI
                     LPAGSTPETRSVRGANLCRISIQQAAQSDVWVVLSVIDNLVKGAAGQAVQNMNIMFGL
                     KETHGLDAIPLLP"
     gene            complement(9728..10021)
                     /locus_tag="WGQ68_001064"
     CDS             complement(9728..10021)
                     /locus_tag="WGQ68_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714881.1"
                     /translation="MKTLVLLLLSFSTTTAFAAYGLGLGQAPKYPADFRAYGYVYSGR
                     QGWVLKTEADAIKLDTLFKRFVYRPSAANQNPVGQAVGQRSDGIPANHTKEKP"
     gene            10383..11537
                     /locus_tag="WGQ68_001065"
     CDS             10383..11537
                     /locus_tag="WGQ68_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0714882.1"
                     /translation="MSIHLDFGISPKTFRQTYLYQKPKLFKGAVRNLESASWKDINEI
                     YQRADPTAPLFHLRKKGAIVPKEEYVESFDDLGKTRYRFIKSVIYEHMKNGASLVYNH
                     INNEPFSDHIARQVARFAGAHTIVSGYLAFGSDESYKNHWDIRDVYAIQLFGKKRWQL
                     TAPDFPMPLYMQQTKDTDISIPEHIDMDIILEAGDVLYIPRGWWHRPIPLGCETFHLA
                     VGTFPPNGYNYLEWLMKKFPTIESLRHSFSDWEQDRTRINDTAAQIAAMIADPINYEA
                     FSEDFLGKERTDTAFHLEQFANPNATPLSDDVRLRLNANNLDTLEKGYLIGNGMKISV
                     DELGKKVLEHIGKNEPLLLKNLLVNFNQGKHEEVRKLIYQLIELDFLELL"
     gene            12091..12807
                     /locus_tag="WGQ68_001066"
     CDS             12091..12807
                     /locus_tag="WGQ68_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein"
                     /protein_id="HEZ0714883.1"
                     /translation="MNRPKQPFFRPEVAVARQTSLTGKVILTRPLSFSLWTTFASISA
                     LLIILFLIFGNYTRKTTVEGQILPASGVIRVYAPDTGTITAKFVEDGEKVKAGDKLFA
                     LSTSRFGAGGSVQQQLKTEAVLKKTLAEQELGRLKLIHGNETRSLKATVERLENQELH
                     ISQQIDGQKRRIRLAEEMLQKYRFLSANDAVPKQEMMNVKAELLEQKAKLDAYRREEV
                     GLLQEIRTQNLTLASLPQAA"
     gene            12872..13366
                     /locus_tag="WGQ68_001067"
     CDS             12872..13366
                     /locus_tag="WGQ68_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEZ0714884.1"
                     /translation="MRSEQIIRAGRSGYIAIPNVEVGQQVDPSKLLLSIVPERTELYA
                     HLYIPSSAAGFIKPKDKVVLRYQAYPYQKFGLASGSVVSVAKTALGRQELSGLGMVSS
                     DLAKSNEPVYLVKIKPDKPTITAYGEEKPLQIGMTLEADILHEKRRLYEWVLELIYSM
                     SGKL"
     gene            13676..13978
                     /locus_tag="WGQ68_001068"
     CDS             13676..13978
                     /locus_tag="WGQ68_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714885.1"
                     /translation="MTWVGGSGNAQQSSYYCEICESAGLNDTGNPDKSLGLPNNTFSN
                     PPGWGATIGALAGSRIGMPEFGTFASHAIENFDWSWYRRYREIAETIEREYSGGLP"
     gene            14262..14510
                     /locus_tag="WGQ68_001069"
                     /pseudo
     CDS             14262..14510
                     /locus_tag="WGQ68_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146716.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            14571..16313
                     /gene="hrpB"
                     /locus_tag="WGQ68_001070"
     CDS             14571..16313
                     /gene="hrpB"
                     /locus_tag="WGQ68_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-partner secretion system transporter HrpB"
                     /protein_id="HEZ0714886.1"
                     /translation="MKFFPAPCLLVILAVIPLKTLAADENDAELIRSMQRQQHIDAEL
                     LTDANVRFEQPLEKNNYVLSEDETPCTRVNYISLDDKTARKFSFLPSVLMKETAFKTG
                     MCLGSNNLSRLQKAAQQILIVRGYLTSQAIIQPQNMDSGILKLRVSAGEIGDIRYEEK
                     RDGKSAEGSISAFNNKFPLYRNKILNLRDVEQGLENLRRLPSVKTDIQIIPSEEEGKS
                     DLQIKWQQNKPIRFSIGIDDAGGKTTGKYQGNVALSFDNPLGLSDLFYVSYGRGLAHK
                     TDLTDATGTETESGSRSYSVHYSVPVKKWLFSFNHNGHRYHEATEGYSVNYDYNGKQY
                     QSSLAAERMLWRNRLHKTSVGMKLWTRQTYKYIDDAEIEVQRRRSAGWEAELRHRAYL
                     NRWQLDGKLSYKRGTGMRQSMPAPEENGGGTIPGTSRMKIITAGLDAAAPFMLGKQQF
                     FYATAIQAQWNKTPLVAQDKLSIGSRYTVRGFDGEQSLFGERGFYWQNTLTWYFHPNH
                     QFYLGADYGRVSGESAQYVSGKRLAGAVAGFRGSHKVGGIFAYDLFAGKPLHKPEGFQ
                     TANTVYGFNLNYSF"
     gene            16406..19357
                     /locus_tag="WGQ68_001071"
                     /pseudo
     CDS             16406..19357
                     /locus_tag="WGQ68_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
BASE COUNT         5079 a         4766 c         5277 g         4819 t
ORIGIN      
        1 tttgttaatc cactataaaa tcccgcgtgt acggcacgcc ccgcgctccg caatgatagg
       61 ggcggtttcg tttgaatcgg cgcaccggga tgaatgcccc cgcgccgtcc cgataccgat
      121 ttgccggttg ttccgtatcg gttgccgccg tgaaatgacg gacggatgca ggaatggcgg
      181 caggcgtgaa tgggaggaaa tgaccgattg acttgtttca aaataaaaaa gtagaatgca
      241 gggcttatcc cgcgtctgtg ggcggttttg cctgcacggt cggttttctg tttaaaataa
      301 aataaaggag aaatgatgct gacgtttatc ggactgctga ttatcggggt catcgtatgg
      361 ctgctgctga cggaaaaagt gtcgcccatc atcgcattaa tcttggtgcc gctgtttggg
      421 gcgttgctgg cggggtttga tgtatcccaa ttaaaagaat tttattcggg cggcaccaaa
      481 tcggtgatgc agattgtgat tatgtttatg ttttccattt tgttttttgg aatcatgaac
      541 gatgtggggc tgttccgtcc gatgataggc ggtttgatta agctgactcg gggtaatatc
      601 gtggcagtga gtgtggggac ggtcttggtg tcggtggtgg cgcagttgga cggggcgggt
      661 gcgacgacgt ttttattggt cgtccccgcc cttttgccgc tttacaagcg tctgcatatg
      721 aatccttacc tgctgttttt gctgctaact tccagtgcgg gattgattaa ccttctgccg
      781 tggggcgggc cgactgggcg ggttgcaagc gtgttgggcg cagatgtggg cgaattgtat
      841 aaacctttgt tgacggtgca aattatcggt gtagtgttta tccttgcgct gtccctgctt
      901 ttgggtgtgc gtgaaaaaag gcggattgtc cgggagttgg gcgcgttgcc cgccgtggcg
      961 gatttgataa agccggtgcc tttgtcggaa gaagaacaaa aattggcgcg tccgaaactg
     1021 ttttggtgga atgtcctgct gtttttggcg gcgatgagcc tgcttttttc gggcatcttc
     1081 ccgccgggtt atgtatttat gctggctgca acggcggcgt tgcttttgaa ttaccgcagc
     1141 ccgcaggaac agatggagcg gatttatgcc cacgccggcg gcgcggtgat gatggcgtcc
     1201 attattttgg cggcaggtac gtttttgggg attttgaagg gtgcggggat gttggacgcg
     1261 atttccaaag acattgtgca tatcctgcca gacgcgctgc tgccttatct gcatattgcc
     1321 atcggtgtgt tgggcattcc gcttgagttg gttttgagta cggacgctta ttatttcgga
     1381 ctgtttccga ttgtggagca gattacctcg caggcgggcg tggcgcccga agcagcaggt
     1441 tatgcgatgt tgatcggcag tatcgtcggc acttttgtta cgccgctttc gccggctttg
     1501 tggatgggct tgggtttggc gaaattgtcg atgggcaaac acatccgtta ttcgtttttt
     1561 tgggcgtggg gtttgtcgct ggcgatattg gccagttcga tagcggcagg aatcgtgcct
     1621 ctgccgtaaa cggcgaagcc gtctgaagcc cgaatcgttc ctgtggtctg tcgcaggctc
     1681 tgcctgcctg cccgtgtgtt gcaatcgcgc cccaatgccg tctgaaggca gttgccggcg
     1741 cggttttttt gtgctgcagt tttgaaaaat accggcaagc gtaaaggccg ctaaattgcg
     1801 gctaattttg gggtgttaga ataggcacgg tttgtctgaa agggaaagtt gatgcgtgtt
     1861 ttgctggtgg aagacgatgc gatgattgcg gaggcggtgt cggcaagttt gaaagacggc
     1921 ggctatgcgg tggattgggt caaaaacggc gcgcggctgc cgctgcccag ccttatgact
     1981 tgatgctgct ggatttgggt ttgccggggc ggacggtttg gatgtttgtt ccgctggcaa
     2041 tgcttgcagg tatgttttcc tactacgaaa ccttccacga aacggaagcg ttgcaggacg
     2101 acctgctccg tcaggcggca ttgtacgtcg cccccgattc caaacccgaa actttgcccg
     2161 agggcgacgg cgatacgcgt attttggtac agatgccgca gcaggaagac cctgttgtca
     2221 gcctgcctgc gcatctggcg gacggtctgc acacgcttca ggcggacggg gacgacgatt
     2281 attaccgcgt ctatatccgc acgaccgaac agggacggat tgccgttatg caggaaaacg
     2341 aataccgtga agatttggcg gcggatgcgg cacggcaaag cgtgttgccc ctgctggcgg
     2401 cactgccgct gatgatactg ctgaccgtgt ggattacgca caaagccatg cgccccgtcc
     2461 gcaaattgtc gcaaagtctc gaacaacgcc gaatcaatga cctgtctgct ttgagtgtgg
     2521 acaatattcc cagtgaaatc agagggttcg taaccgccat caacctgctt ttgaaacgtg
     2581 cggatgaaga catccgccac cgtcagcggt ttgtcgccga cgcggcacac gaattgcgta
     2641 cgccgatgac agccctttcc cttcaggcgg aacggctcaa caatatgtcg ctcccacccg
     2701 atgcagcgcg gcagcctgcc gttttgcagc agagcatcag gcgcaacaaa cacctgctcg
     2761 aacagctttt ggcactggcg cgttcgcagt cggacgaaac ccctctgacg aaaacgacat
     2821 tcggtctgca aagccgtttc cgccaagtgt tgcaggaact gatgccgctg gctttggaaa
     2881 aacgtcagga catcggtgtg gcggtcagag gcgatgtcga agtgtctgcc gacgaaacag
     2941 aaatctatac gctgattaaa acctttgccg ataacgcggt acgttacacg ccgcctgaag
     3001 gcaggataga tttgggtttc acagacgaag ggaaatatct tgccgtgtgg gtggaagata
     3061 acgggaacgg cattcccgaa tccgaacgcg cccgcgtcct cgatccgttt taccgtattt
     3121 tgggaacgga gcagcagggg acggggctgg gtctgtccat cgccgacacg cttgccaaaa
     3181 aatacggcgg acatttggaa ctgaccgaca gccgacgttt cggacacggt ctgttgatac
     3241 gggcgttgtt ggacaaggaa accctgaaat agacagatgc cgtctgaagg cttcagacgg
     3301 cattttttac atcagcacta tatcgtattg ttcctgcgtg taagcggttt cgaccgccag
     3361 agaaatcggt ttgccgatga aatctatcag cattgccaag gattgcgatt cttcgtccaa
     3421 aaacaaatcg atgacgttgg gggcggcgag gatgcggaaa ctttcggcat cgtaacggcg
     3481 cgcttcgcgg acgatttcgc gctggatttc gtagcacacg gtttgcggcg ttttcagacg
     3541 gcctctgcct tggcaggaag ggcagggttc gcagaggact tggtttaagt tttcgcgcga
     3601 gcgtttgcgc gtcagctcga ccagtcccag gctggtaaaa ccgtgcaggg taacgcgggt
     3661 acggtcgaag gcgagggctt tggcaagctc ctgcaacacg gcttcgcggt ggctttcctg
     3721 cgccatatcg atgaagtcga tgatgatgat gccgccgagg ttgcgcagcc tcaattcgcg
     3781 ggcgatggtg tggcaggctt cgaggttggt gcggaaaatc gtttcatcga aattgcgtgc
     3841 gccgacgaag ccgcctgtgt tcacgtctat cgtggtcatc gcttcggtgg attcgataat
     3901 caggtagctg ccgaagttga ggttgacgcg cggttgcagg gcgcggctga tttcctgttc
     3961 gacgttgtgg gtttcaaata gcgggcgttc gcctttgaac aattctatcc tgcccaatgc
     4021 gccgtggacg tattgttcgg caaaacgcgt catgcggccg tggtttacgg tggaatcgac
     4081 gaggattttt tgcgtgtcgc agccgaccat atcgcgcaac acgcgcaaac tcaagggcaa
     4141 atcctgataa agcagggttt ccggcggccg gattttcgcc tgttcttgga tgtgttccca
     4201 cactttggtc aggtagtcga tgtcggactg gagctgttcg tcggtggcgt tttcggcgtt
     4261 ggtgcggatg atgtagcccc ggcaggcatt ttccggcagg agcttgtcga ggcgttcgcg
     4321 caggctgctg cgttcggcat cgtcttcgat gcgttgggac acgccgatgt ggtcttcttg
     4381 cggaagatgg acgaggaaac gccccgccag cgagatttgg gtggaaagcc gcgcgccttt
     4441 ggtgttgatc gggtctttga tgacctgcac caaaacagac tgcccttcaa acagcatatg
     4501 ttcgatgcgc tgggtttctt cggggttgcg gcgttgttcg aggacatcga cgatgtgtaa
     4561 aaacgccgcg cgttccaagc cgatgtcgat aaacgcgctc tgcatcccag gcagcacgcg
     4621 gcgcaccacg cccaaataga tattgccgac taggctgtgt tcgctgttgc gctcgatgtg
     4681 cagctcgcag atattgtttt cctccaacac cgctacgcgc gtttcctgcg gcgtgatgtt
     4741 gaccaatatc gtttcgggcg ggcgcgcgat gtctttgggg atggggagtc ctgacaacat
     4801 ggtttttcct gaaaatgtaa taaaaatatt tttgcatccg ttccgccgcg cagcgcgggt
     4861 gcaaaaatat ttttcacaat gtctatcata ctttaaaaaa gaaactttga cacattccgc
     4921 acatgccgtt tgaacgtgca tctgttccgc acttgccaaa cgggggaaat gcccctatat
     4981 tgattatcat tgcaaaactt tcggaaaccc aatatgcaga ccgttaccat gtacacaggt
     5041 ccgttttgcc cctactgcac gatggcgaaa aggctgctgc acgcggcagg tgtcggacat
     5101 atcgacgaaa tccgtgtcga tgcaagtccc gaagcctttg ccgaaatgca gcagctttcc
     5161 ggacagcgca gcgtgccgca gattttcatc ggcgaaacgc acgtcggcgg atttaccgac
     5221 ctctaccgcc tccagcagga aggcgggctg gacggactgc tgaaccctta acccccaact
     5281 aggaaaacaa aatgagcgaa gaactgcaac ccgtattcag catcgagcga ctgtatgtca
     5341 aagacttgtc tttggaagtg ccgcacgcgc cgcaaatctt tttggaacag ggcgagcccg
     5401 aagtggatat gcgcgtttcc accggcagcc aaaagctgga agacggctac tacaacgtgg
     5461 acgttaccgt aaccgttacc gccaagctgg ataacgagcg cacgatgttt ttgaacgaag
     5521 taacccaaag cggcatcttc cgtttggaaa acatccccga agaagatgcc gacctgctgc
     5581 tcggcgtggc gtgtccgaac atcctcttcc cttacgcgcg cgaagcggtt tccggtacgg
     5641 taacgcgcgc cggcttcccg cccgtcctgc tcgcgccgat taattttgag gcgatttacc
     5701 aacaacagca ggaagccgaa gccgccgggg cttgattcct gcctgtcgat gccgtctgaa
     5761 tccgtttcag acggcatttt tgttttcgga taaaataggc gcgtccaaag gaatctgcct
     5821 atgatgtcgc ccgaaacccg aaaacagctc aaaatcaccg atgtttccgc caagaagctc
     5881 gacaaactca acctccatac cgcgtgggat ttggtgttgc acctgccgct gcgttacgag
     5941 gacgagacgc acattatgcc gattaaggac gcgccgattg gcgtgccgtg tcaggtcgag
     6001 ggggaggtta tccatcagga agtaacgttc aaaccgcgca agcagctgat tgtccaaatc
     6061 gccgacggct ccggcagcgt cctttttctg cgcttcatcc acttttacgc cagccatcag
     6121 aaacagacgg cggtcggcaa acgcatccgc gccgtgggcg aaatcaaaca cggcttttac
     6181 ggcgacgaga tgattcatcc caaaatccgc gatgccgagg gcggcggttt ggcggaaagc
     6241 ctcacgccgg tttacccgac cgtaaacggt ttgaaccagc ccactttgcg ccgtattatt
     6301 cagacggcgt tggacgttac gccgctgcac gacacgctgc ccgatgccct gctgtgccgt
     6361 ctgaagctgc cacacctcgc cgaaagcctg cgtcttttgc attcgccgcc gccgagtttc
     6421 acgattcatc agctttcaga cggcacgctg cctgcatggc aacggctcaa attcgacgaa
     6481 cttttggcgc aacagctttc gatgcgcttg gcgcgacaga agcgtatcgg cggcacggcg
     6541 gcggcattgg gcggcgacgg cacattgacc caagccctgc gccaagccct gccgtttgcc
     6601 ctgaccgatg cgcaagaaaa agttgtttcc gaaatctgcc gcgatatggc gcaaacctac
     6661 ccgatgcacc gcctgctgca gggcgatgtc ggcagcggca aaaccattgt ggctgctttg
     6721 tctgctttga cggctatcga atccggtgcg caggtggctg taatggcgcc cactgaaatc
     6781 cttgccgaac agcattttat taaatttaaa caatggctcg aacctttggg cattgaagtt
     6841 gtccgccttt ttggcagttt gcgtaaaaaa gccaaagacg aagccaaagc caaactggct
     6901 gacggcagcg tcaaaatcgc cgtcggcacg cacgccctgt tttcagacgg cgtggcgttt
     6961 cacaatttgg gcttgaccat tgtggacgaa cagcaccgtt tcggcgttgc ccaacgcctc
     7021 gccctcaaaa acaaagggcg cgaagtccat cagctgatga tgtccgccac gcccatcccg
     7081 cgcacgcttg cgatgagttt tttcgccgat ttggacgtgt ccgtcatcga cgaattgccg
     7141 cccgggcgca caccgattaa aacgcgcctc gtcaacaacg tccgccgtgc cgaagtcgaa
     7201 ggcttcgtcc tcggcacttg ccgaaaaggg cggcaggcgt attgggtctg cccattgatt
     7261 gaagaaagcg aaaccctgca actgcaaacc gccgccgaaa ccctcgcccg gcttcagacg
     7321 gcattgcccg aactcaatat cggactggta cacgggcgca tgaaggccgc cgaaaaggcc
     7381 gaagtgatgg cgcggttttc ttcggggggg ctgaacgtct tggtcgccac caccgtcatc
     7441 gaagtcggcg tagatgtgcc caatgccgcc ctgatggtca tcgaacacgc cgagcgcatg
     7501 ggcttggcgc agctgcacca attacgcggt cgggtcgggc gcggcgcggc agaaagcgtg
     7561 tgcgtcctcc tgtttgccga acccttgggc gaactcgcca aagcgcggtt gaaagtgatt
     7621 tacgaacaca ccgacggctt tgaaatcgcc cgccaagacc tcaatatccg cggccccggc
     7681 gaatttctcg gcgcgcgcca aagcggcgtg cctatgctgc gcttcgccaa gcttgaagaa
     7741 gacttacacc ttttggaaca agtgcgcgaa atcgccccga tgctgattga gcaaaacccc
     7801 gaaatcgtcg aagcgcattt ggcaaggtgg ctttccggca gggaagggta tttgggcgtg
     7861 tgagcaaaat gccgtctgaa atattggaaa aaagtgtaac ggaaatctga ttttgcatta
     7921 tttttgacac attttcatga ttttaaatca cgtcattccc aagtaggcgg aaaccggcaa
     7981 tgagaagccg cagagactta ccaggaaaaa cggcaagccc tccgccgtca ttcccgcgca
     8041 ggcgggaatc tagaaatgaa aaaccacggt gttatcggca atgactgaaa ccggagaaat
     8101 gacgaaattg aagcgtgcgg aaatgtgtcg gaaatcgctg aagccggata aactagattc
     8161 ccgcttttgc gggaatgacg ggattttagg tttttggttt gttttcgcgg caatgatggg
     8221 attttaggtt tttggtttgt tttcacggca atgatgggat tttaaattgc aggtatttac
     8281 cgggaaaaac ggcaagccct ccgccgtcat tcccgcgcag gcgggaatct agaaataaaa
     8341 aaccacggtg ttatcggcaa tgactgaaac cggagaaatg acgaaattga agcgtgcgga
     8401 aatgtgtcgg aaatcgctga agccggataa actatagtgg attaaattta aatcaggaca
     8461 aggcgacgaa gccgcagaca gtacagatag tacggcaagg cgaggcaacg ctgtactggt
     8521 ttaaatttaa tccactatag attcccgctt ttgcgggaat gacggtattt tagatttttg
     8581 ttttcgcgga ataatgagcc aaaagccgtc cgaacccaaa aaaaccgccg ctttcagatg
     8641 gcatcaaaaa accgcaggca cgatgcctgc ggtttgccgt ttgcgtttca ggggagcagg
     8701 gggattgcgt ccaagccgtg tgtttccttc agtccgaaca tgatgttcat attttgcacc
     8761 gcctgtcccg ccgcgccttt gacgaggttg tcgatgacgg aaaggacgac ccacacatcg
     8821 gattgcgccg cctgttggat gctgatgcgg cagaggtttg cgccgcgcac gctgcgggtt
     8881 tcgggtgtcg aaccggcggg caggatgtcc acgaacaggc tgtcgcgata atagtcgcgc
     8941 aaaacggttt cggggttgct gccgtctgaa aggtggaggt aaacggtggc gtgcataccg
     9001 cgtatcatcg gcgcgaggtg cggcgtgaac acgaatcctt cggcgatgcc gtcctgaagc
     9061 ccggcgatgg tctgcctgat ttcgggcagg tggcggtgtc cggctatgcc gtaggctttg
     9121 aagttgtcgc cggcttcgca caacagcgaa ccgacattgc ctttcctgcc cgcgccggac
     9181 acgccggatt tgcagtcggc aatcagcggc ataccgggct tcagacggca ttgccgcaac
     9241 agcggcacga gcggcaggga tacgcaggtc gggtagcagc cggggttggc gacgaggcgt
     9301 gcctgtgcga cggcttcgcg gttgagttcg ctcaatccgt acacggcttg ggaaacgagg
     9361 tcgggggcgg cgtgggtcat gccgtaccag tgttcccagg tcggaatgtc ccgtatgcgg
     9421 aagtcggcgg aaaggtcgat gacgcgcacg ccctgttcaa tcaggcgcgg cgcgtctttc
     9481 atggcgatgc cgttgggcgt ggcgaagaag acgatgtcgc attgttccaa acctgcctcg
     9541 tcgggcgttt ggaaggcgag gccgtacacg ccgcgcaaac tcggaaagta atcggcaact
     9601 gcagttcccg cttcgctgcg gctggttacg gcggcgactt cgacatcggg atgggcggca
     9661 agcaggcgca gcagttccac gcccgtgtag cccgtcgcgc cgacaatgcc gactttgatt
     9721 tttttactca tggtttttcc tttgtgtggt tggcgggtat gccgtccgaa cgctgtccga
     9781 cggcttgtcc gacggggttt tgattggcgg cggaaggacg gtacacaaag cgtttgaaaa
     9841 gcgtgtctaa tttaatggca tccgcctctg tttttaaaac ccagccctgc cgtccggaat
     9901 aaacataacc gtaggcgcga aagtcggcag gatatttcgg tgcctgcccc aaacccaaac
     9961 cgtatgcggc gaaagcggtg gtcgtggaga aggaaagcag gaggaggacg agggttttca
    10021 taaggttcca aaccgaacgg ggcgatgaag cccgattata gcaaaaggtc gtctgaaaac
    10081 cgttttcaga cgacctttta tttaattaca ataaaaatgt taacaacaaa aaacaaaccg
    10141 ctttttttcg tttggaaaca aaacaaattt ttatagtgga ttaaatttaa accagtacag
    10201 cgttgcctcg ccttgccgta ctatttgtac tgtctgcggt ttcgtcgcct tttcctgatt
    10261 tttgttaatc cactataaca aaattggcaa caattccttg acgcacacac aaaaaaaact
    10321 ttaatatttg taaatatatg taacattata aattttaaat attttctgag taaggggaag
    10381 taatgagcat ccatctcgac ttcggcatta gtcctaaaac gttccgacag acttatctgt
    10441 atcaaaagcc caagctcttt aaaggagcgg ttcggaatct cgaatccgca tcttggaaag
    10501 atatcaacga gatataccaa cgagcagacc caaccgcacc gctgtttcat ctgcgtaaaa
    10561 aaggcgcaat cgttcctaaa gaagaatacg tcgaaagttt cgacgatttg ggcaaaactc
    10621 gctaccgttt tattaaatcc gttatctacg aacatatgaa gaatggtgcg tcgttagtct
    10681 ataaccatat taacaacgag ccgttttcag accatatcgc ccgtcaagtc gcccgctttg
    10741 ccggcgcaca tactattgtt agtggatatc ttgcttttgg cagcgacgaa tcttataaaa
    10801 accattggga catccgagat gtgtatgcca tccagctttt cggcaagaaa cgttggcaac
    10861 ttactgcccc tgatttccct atgccattgt atatgcaaca gactaaagat actgatattt
    10921 ccattcctga acatatcgat atggatatta tccttgaagc aggtgatgtc ctctacatcc
    10981 cgcgcggttg gtggcacaga cctatcccgc tcggctgtga aaccttccac ctcgctgtcg
    11041 gtacctttcc gcccaacggc tataattacc tcgagtggct aatgaagaaa ttccccacca
    11101 tagaaagtct gcgccacagt ttctcagact gggagcaaga taggacgcgt atcaacgata
    11161 ctgccgcaca aattgctgcc atgattgccg accccatcaa ttacgaagcc ttcagtgaag
    11221 acttcctcgg caaagaacgt accgataccg cttttcatct cgaacagttc gcgaatccca
    11281 acgctactcc gctttcagac gacgtcaggt tgagactaaa tgccaataat ttggatacgt
    11341 tggaaaaggg atatttgatt gggaatggga tgaagataag cgtagatgag ttggggaaaa
    11401 aagtgttaga acatatcggt aagaatgaac cgttattgtt gaaaaatcta ctggttaact
    11461 tcaatcaggg aaaacatgaa gaagttagga agttgattta tcagttgata gagttagatt
    11521 ttctggaact tttgtgaggg attctatgaa aaactggaag caatttacat ttttcgtaat
    11581 attagtaata gcttgttatc aattgctata ttttttatca gatatgtttc ttctcgacta
    11641 cattaacaaa tatagttgga atttgaattt tattcagggt actttgaatt ttttttcaat
    11701 atatctgcca tatgtttttg ttagccgaat ttttaggaat accaaccaag aaaaggagta
    11761 taaaaatgat tgaacttcaa cttcatgaat tgaagctggt ttcaggggga ggtcctgtaa
    11821 cagacaatat agctggaaat gtagctaatg ctgccacaac caaaggaggt cggtgtatac
    11881 aatctgactc gtgattgggt aaatgatgcc gttaatgcac ctccttataa cggaagacca
    11941 atctttgaga ttgaacatgg attaactgct cccgcaacaa aagcagataa atcaggaaac
    12001 ggctacactg acggtacaga ttactgctga tatttctcat ctttcagacg atctctaggg
    12061 gtcgtctgaa actttcttaa cttcaatttt atgaatagac ccaagcaacc cttcttccgt
    12121 cccgaagtcg ccgttgcccg ccaaaccagc ctgacgggta aagtgattct gacacgaccg
    12181 ttgtcatttt ccctatggac gacatttgca tcgatatctg cgttattgat tatcctgttt
    12241 ttgatatttg gtaactatac gcgaaagaca acagtggagg gacaaatttt acctgcatcg
    12301 ggcgtaatca gggtgtatgc accggatacg gggacaatta cagcgaaatt cgtggaagat
    12361 ggagaaaagg ttaaggctgg cgacaagcta tttgcgcttt cgacctcacg tttcggcgca
    12421 ggaggtagcg tgcagcagca gttgaaaacg gaggcagttt tgaagaaaac gttggcagaa
    12481 caggaactgg gtcgtctgaa gctgatacac gggaatgaaa cgcgcagcct taaagcaact
    12541 gtcgaacgtt tggaaaacca ggaactccat atttcgcaac agatagacgg tcagaaaagg
    12601 cgcattagac ttgcggaaga aatgttgcag aaatatcgtt tcctatccgc caatgatgca
    12661 gtgccaaaac aagaaatgat gaatgtcaag gcagagcttt tagagcagaa agccaaactt
    12721 gatgcctacc gccgagaaga agtcgggctg cttcaggaaa tccgcacgca gaatctgaca
    12781 ttggccagcc tcccccaagc ggcatgagac agaacaaagc tggcttgaac gcaccattgc
    12841 cgatatttct caagaagttt tggattttga aatgcgctct gaacaaatca tccgtgcagg
    12901 acggtcgggt tatatagcaa taccgaacgt cgaagtcgga cagcaggttg atccttccaa
    12961 actgctcttg agcattgttc ccgaacgtac cgagctatat gcccatctat atatccccag
    13021 cagtgcagca ggctttatca agccgaaaga caaggttgtc ctacgttatc aggcatatcc
    13081 ctatcaaaaa ttcgggcttg cttccggcag tgtcgtatca gtagcaaaaa cggcactggg
    13141 cagacaggaa ttgtcgggat tgggcatggt atcctccgat ttggcgaaga gcaacgaacc
    13201 tgtttatctc gtgaaaataa aacccgacaa accaaccatc actgcatacg gtgaggaaaa
    13261 accgctgcaa atcggcatga cgttggaagc agacatcctg cacgagaaac ggcggctgta
    13321 cgaatgggta ttggagctga tttatagtat gtcgggcaaa ctgtaagact tgaggctgaa
    13381 agataaagaa gttgtctgaa accttttcag acgaccagaa gacaataaaa aacacaaact
    13441 tcaaaactgc ctgaaaccat ccccttttac aggcactccg tttgtaaaag caaggatagg
    13501 gcatctgccc ttttgattgt ttgattaacg atacaaggag tttcaaaatg agagttttat
    13561 agtggattaa caaaaaccag tacagcgttg cctcgccttg ccgtactatt tgtactgtct
    13621 gcggcttcgt cgccttgtcc tgatttaaat ttaatccact atatgtgttc atgaaatgac
    13681 ttgggtcgga ggctcaggta atgcgcaaca aagctcatat tattgcgaaa tttgcgaatc
    13741 tgcagggctt aacgatacgg gaaatcctga taaatcttta ggattgccaa acaatacgtt
    13801 cagtaatccg cctggttggg gagctacaat cggagcttta gcaggtagcc gcatagggat
    13861 gcctgaattt ggtacgtttg cgagccatgc cattgaaaat ttcgactggt catggtatcg
    13921 acgttatagg gaaattgccg aaacgattga acgagaatat tcaggcggtt tgccttaata
    13981 gttgaggagg tcatgatgtt tgccaaacat tatcaattca tcgcactcgg catcatgctg
    14041 cttctttata tgttgattct ctatacgacc gatttttcca atctgacgta ttggatgctg
    14101 ttttttatct gttttattac aggaaaaata ttagctcgtt tgttagagaa aagctttaaa
    14161 taaaatagca gctagtcgca aaaggtcgtc tgaaaccttt tcaggcggcc tttctaaaat
    14221 acatccaact tcctaatccc tatttttcaa aaaggaaatc tatgccccat ctgcaaaacc
    14281 tgtctttggg cttaaagaaa aagctgcctg ttatcctgta aacagaaata tcagaatgcg
    14341 gcttggcatg tctggcggct gtggcgggat ttcatggttt ccatacggat ttacgcgcac
    14401 tgcgttcaaa atactgtccg agacctttgc aaaattcccc aaaatcccct aaatgtcttg
    14461 gtgggaattt aggggatttt ggggaatttt gcaaaggtct cattctataa ctgtaaatac
    14521 ttttaaattt atgacaaaat agtaaatatt gctaaaataa tattgatgtc atgaaatttt
    14581 ttcctgctcc atgtctgttg gttatcctgg ctgtcatacc ccttaaaacc ttagctgccg
    14641 atgaaaacga tgcagaactt atccgttcca tgcagcgtca gcagcacata gatgctgaat
    14701 tgttaactga tgcaaatgtc cgtttcgagc aaccattgga gaagaacaat tatgtcctga
    14761 gtgaagatga aacaccgtgt actcgggtaa attacattag tttagatgat aagacggcgc
    14821 gcaaattttc ttttcttcct tctgtgctca tgaaagaaac agcttttaaa actgggatgt
    14881 gtttaggttc caataatttg agcaggctac aaaaagccgc gcaacagata ctgatcgtgc
    14941 gtggctacct cacttcccaa gctattatcc aaccacagaa tatggattcg ggaattctga
    15001 aattacgggt atcagcaggc gaaatagggg atatccgcta tgaagaaaaa cgggatggga
    15061 agtctgccga gggcagtatt agtgcattca ataacaaatt tcccttatat aggaacaaaa
    15121 ttctcaatct tcgcgatgta gagcagggct tggaaaacct gcgtcgtttg ccgagtgtta
    15181 aaacagatat tcagattata ccgtccgaag aagaaggcaa aagcgattta cagatcaaat
    15241 ggcagcagaa taaacccata cggttcagta tcggtataga tgatgcgggc ggcaaaacga
    15301 ccggcaaata tcaaggaaat gtcgctttat cgttcgataa ccctttgggc ttaagcgatt
    15361 tgttttatgt ttcatatgga cgcggtttgg cgcacaaaac ggacttgact gatgccaccg
    15421 gtacggaaac tgaaagcgga tccagaagtt acagcgtgca ttattcggtg cccgtaaaaa
    15481 aatggctgtt ttcttttaat cacaatggac atcgttacca cgaagcaacc gaaggctatt
    15541 ccgtcaatta cgattacaac ggcaaacaat atcagagcag cctggccgcc gagcgcatgc
    15601 tttggcgtaa cagacttcat aaaacttcag tcggaatgaa attatggaca cgccaaacct
    15661 ataaatacat cgacgatgcc gaaatcgaag tgcaacgccg ccgctctgca ggctgggaag
    15721 ccgaattgcg ccaccgtgct tacctcaacc gttggcagct tgacggcaag ttgtcttaca
    15781 aacgcgggac cggtatgcgc caaagtatgc ccgcacctga agaaaacggc ggcggtacta
    15841 ttccaggcac atcccgtatg aaaatcataa ccgccggatt ggatgcagcg gccccgttta
    15901 tgttgggcaa acagcagttt ttctacgcaa ccgccattca agctcaatgg aacaaaacgc
    15961 cgttggttgc ccaagataaa ttgtcaatcg gcagccgcta caccgttcgc ggatttgatg
    16021 gggagcagag tcttttcgga gagcgaggtt tctactggca gaatacttta acttggtatt
    16081 ttcatccgaa ccatcagttc tatctcggtg cggactatgg ccgcgtatct ggcgaaagtg
    16141 cacaatatgt atcgggcaag cggctggcgg gtgcagtggc cggcttcaga ggcagccata
    16201 aagtaggcgg tatatttgct tatgacttat ttgccggcaa gccgctgcac aagcccgaag
    16261 gttttcagac ggccaacacc gtttacggct tcaatttgaa ttacagtttc taaccctctc
    16321 ctgcattttt taccgacatt cagacagtct ttcatcattt acaggccgtc tgaagcgatt
    16381 acaaacatca tttaaaggga taaatatgaa caaaggtctg catcgtaccg ttttcagcaa
    16441 aaagcacaac actatggttg cagttgccga aaccgccaac agtcagggta aaggcaaaca
    16501 ggcaggaact tccgctccgg cttcgccaca aatttcagac ggcctttaca gtaaactcaa
    16561 aaccaccctt aaaaccttgg tctgctcttt ggtttccctg agtatggtat tgcctgccca
    16621 tgcccaaatt accaccgaca aatcagcacc taaaaaccag caggtcgtta tccttaaaac
    16681 caacactggt gcccccttgg tgaatatcca aactccgaat ggacgcggat tgagccacaa
    16741 ccgctatacg cagtttgatg ttgacaacaa aggggcagtg ttaaacaacg accgtaacaa
    16801 taatccgttt ctggtcaaag gcggtgcgca attgattttg aacgaggtac gcggtacggc
    16861 tagcaaactc aacggcatcg ttaccgtagg cggccaaaag gccgacgtga ttattgccaa
    16921 ccccaacggc attaccgtta atggcggcgg ctttaaaaat gtcggtcggg gcatcttaac
    16981 taccggtgcg ccccaaatcg gcaaagacgg tgcactgaca ggatttgatg tgcgtcaagg
    17041 cacattgacc gtaggagcag caggttggaa tgataaaggc ggagccgact acaccgagat
    17101 acttgctcgt gcagttgctt tgcaggggaa attacagggt aaaaacctgg cggtttctac
    17161 cggtcctcag aaagtagatt acgccagcgg cgaaatcagt gcaggtacgg cagcgggtac
    17221 gaaaccgact attgcccttg atactgccgc actgggcggt atgtacgccg acagcatcac
    17281 actgattgcc aatgaaaaag gcgtaggcgt caaaaatgcc ggcacactcg aagcggccaa
    17341 gcaattgatt gtgacttcgt caggccgcat tgaaaacagc ggccgcatcg ccaccactgc
    17401 cgacagcact gaagcatcac cgacttatct ctccatcgaa accaccgaaa caggcgcagc
    17461 aggcgcattt atctccaatg gcggccggat agagagcaaa ggcttattgg ttattgatac
    17521 cggggaggac atcggtttgc gtaacggagc ggtcgttcaa aacaacggca accgccctgc
    17581 tgctacggta ttaaatgccg gccgtaattt ggttatcgaa agcaaagcaa atgtgaacaa
    17641 tgctaaaggt tcgaccaatc tgtcgaccgg cggtcgtact acgatcaatg atgctactat
    17701 tcaagcgggc agttccgtgt acagctccac caaaggcgat actgaattgg gtgaaaatac
    17761 ccgtattatt gctgaaaacg taaccgtatt gtccaacggc agtattggca gtgctgctgt
    17821 aattgaggct aaagacactg cacacattga agcgggtaaa ccgctttctt tagagacttc
    17881 aaacgttgct tctaacatcc gtttgaacaa cggtagcatt aaaggcggaa agcaggtggt
    17941 attgatggct gatgacgaca ttcaggcaaa agcctcccac ctgaatgcgt ccggcaatct
    18001 gtatattcat gcaggtaagg atctggattt gaatgccgat aaagacttat caacacaaag
    18061 tatcagcctg agggcagaca acaccgccct catcagcagc aacggtaaca ccttgactgc
    18121 agaaaagaat ctggatattc aggcaggtag cttgagtgtg cgtcaaagta atctgcaatc
    18181 cagtggcggt aatgtgcaga tgagcgcgac taaaggcaac atcagcttga atcagagttg
    18241 gataaatgcc agtcagaata ttgatacggc agcacttcaa ggcaatatta tttcagacgg
    18301 tctgactgct gttgccgaag ttggacgcgt atctcttctt gccaacggca atgttgattt
    18361 caacggtcta aataccttga ttgcggaagg agacattaat gctggttcag tcggtaaagg
    18421 ccgtctgaaa atggacaata ccgatattta tgcttctgca ggcgatgtga aactggttgc
    18481 cggaggtcaa ttagatttag gcaacggcac cgttaacggc ggtcatatca gcttagacag
    18541 caataaaggc agcatggtgg tgcagaatgt acacctgaat gcccgcgcat cactgaaagt
    18601 ggatgcggat caaactttaa ccattaataa cagcaagctc aattccgatc acaataccca
    18661 gattaataca aatcatggtc atatgacgct taatcagctt gatgctcatt cacgtcgtca
    18721 tatgagtatc agtgcacagg gtaaaggcaa aggaaaagac agcggtcaaa ttttacaaaa
    18781 cgaccagcaa aacagtaaaa gtactttggc ggcagatggt gtattgtcat tgaacagcag
    18841 cgcattacag gttttggaca atactaccct gcgcggtggc gcgataaaca tcaaagctgg
    18901 cggaggcatc atcaaacggg ggcatatcga ttgggaaacc caagatacgg caactatgcg
    18961 ttcagcggaa ctgaaaccac tgtccggtat gatgtcgata gaatcgggcg gtgataaccc
    19021 tttaaccgtt gaacccggta accgtatcgt ttctgcaggt gatttggccg tgaaacacaa
    19081 cggcacattc caaatcagtg ccagagcagg aaataacggc aatccgagtg cgcaaacagc
    19141 cagcgtttca gctaaaggca atatcgggat tgtggcggga gaggtggata ttgatgcagc
    19201 aaatatggcc gcagggaaag atttggcttt agtggcaact aaagggaata tttcactaaa
    19261 cagtattagg aatacgttta gtaactatca attgaaaaca gataaacaca atatcacaca
    19321 gcaacttact gatgtagagc aggagcttag taagttaacg agcgatccta aatatcgtaa
    19381 agcacaggat cttccacaaa tgttgaggcg taaatataag aggcgtgaca aagtattcgg
    19441 tgatagtgag gccagattac gtggtttacg agcaaagata aatgctgctg atgaggcttg
    19501 ggcagaacgc caatccccag taaaagcgtt acttgaacgt aaacaattgt tacaacaagc
    19561 ccttttaaca gtttcccaac cgggtagtgg tcacgaaaac caaggtagca cccttagcgg
    19621 tcaaaatatc aaactgctcg ctgcaggtgg tatccgtatc cagggctcca aagttgctgc
    19681 cacccaacag gcaaatatcc aagctgcagg atttttacct gcaccagccg cagaggaact
    19741 gcaagaaggt cgacttcaat cagccattga catcagcggt gtacttgata cttttgaata
    19801 tggtcagcaa ggcagcgata aatacggcta tgccattttc agcaggccat ctgaaatttc
    19861 aggaaaaaca ggtgttaccc tctctgcacc aaatgccaat gagaacagcc gcatcagtct
    19921 gagtgcggca aacatcgaag c
//
LOCUS       DAWFHY010000035        19776 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.035, whole genome shotgun sequence.
ACCESSION   DAWFHY010000035 DAWFHY010000000
VERSION     DAWFHY010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19776
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.035"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            46..1224
                     /locus_tag="WGQ68_001072"
     CDS             46..1224
                     /locus_tag="WGQ68_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEZ0714887.1"
                     /translation="MAFLKLTEQNVQGKTVLIRADMNVPFKDGKISDDTRIRASLASV
                     KYCLDNGASVIVMTHLGRPTEGEFHPEDDVAPVAAHLGGLLGKDVKVLNDWRENKPAL
                     NAGDVVMLQNVRINKGEKKNDLELGKAYASLCDVFVNDAFGTAHRAQASTEAVAQAAP
                     VACAGVLMAGELDALGKALKQPARPMVAIVAGSKVSTKLTILESLADKVDQLIVGGGI
                     ANTFLLAEGKAIGKSLAEHDLVEESKKIMAKMAAKGGSVPLPTDVVVAKAFAADAEAV
                     VKDIADVAEDEMILDIGPKSAAALADLLKAADTVVWNGPVGVFEFDQFAGGTKALTEA
                     IAQSKAFSIAGGGDTLAAIAKFGVTDQIGYISTGGGAFLEFLEGKELPAVAALEKRGE
                     "
     gene            complement(1292..1540)
                     /locus_tag="WGQ68_001073"
     CDS             complement(1292..1540)
                     /locus_tag="WGQ68_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEZ0714888.1"
                     /translation="MLTSEQVKAMIEGVAKCEHIEVEGDGHHFFAVIVSSEFEGKARL
                     ARHRLIKDGLKAQLESNELHALSISVAATPAEWNAKAQ"
     gene            complement(1631..2548)
                     /gene="ftsX"
                     /locus_tag="WGQ68_001074"
     CDS             complement(1631..2548)
                     /gene="ftsX"
                     /locus_tag="WGQ68_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HEZ0714889.1"
                     /translation="MSIIHYLSLHVESARTALKQLLRQPFGTLLTLIMLAVAMTLPLF
                     MYLGIQSGQSVLGKLNESPQITVYMETAAAQSDSDTVRSLLVRDKRLDNIRFISKEDG
                     LEELQSNLDQNLISMLDGNPLPDVFIVTPDPATTPAQMQAIYRDITKLPMVESANMDT
                     EWVQTLYQINEFIRKILWFLSLTLGMAFVLVAHNTIRLQILSRKEEIEITKLLGAPAS
                     FIRRPFLYQAMWQSILSAAVSLGLCGWLLSAVRPLVDAIFKPYGLNIGWRFFYPGEIA
                     LVFGFVIALGIFGAWLATTQHLLGFKAKK"
     gene            complement(2545..3195)
                     /gene="ftsE"
                     /locus_tag="WGQ68_001075"
     CDS             complement(2545..3195)
                     /gene="ftsE"
                     /locus_tag="WGQ68_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEZ0714890.1"
                     /translation="MIRFEQVSKTYPGGFEALKNVSFQINKGEMIFIAGHSGSGKSTV
                     LKLISGITKPSRGKILFNGQDLGTLSDNQIGFMRQHIGIVFQDHKILYDRNVLQNVIL
                     PLRIIGYPPRKAEERARIAIEKVGLKGRELDDPVTLSGGEQQRLCIARAVVHQPSLLI
                     ADEPSANLDRAYALDIMELFKTFHEAGTTVIVAAHDETLMADYGHRILRLSKGRLA"
     gene            complement(3390..3872)
                     /locus_tag="WGQ68_001076"
     CDS             complement(3390..3872)
                     /locus_tag="WGQ68_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEZ0714891.1"
                     /translation="MKYLNLAAITLAATFAAHTASADELAGWKDNTPQSLQSLKAPVR
                     IVNLWATWCGPCRKEMPAMSKWYKAQKKGSVDMVGIALDTSDNIGNFLKQTPVSYPIW
                     RYTEANSRNFMKSYGNNVGVLPFTVVEAPKCGYKQTITGEVNEKSLTEAVKLAHSKCR
                     "
     gene            3996..4349
                     /gene="arsC"
                     /locus_tag="WGQ68_001077"
     CDS             3996..4349
                     /gene="arsC"
                     /locus_tag="WGQ68_001077"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271974.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEZ0714892.1"
                     /translation="MPEIKIFHNPRCSKSRAALSLLEERGIAAEVVKYLDTPPDLSEL
                     KDIFNKLGLASARGMMRVKDDLYKELGLDNPNLDNDALLRAIADHPALLERPIVLANG
                     KAAVGRPLENIEAVL"
     gene            4346..5002
                     /locus_tag="WGQ68_001078"
     CDS             4346..5002
                     /locus_tag="WGQ68_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEZ0714893.1"
                     /translation="MNKRLFCSRNGLRYYLLGGFCLSVFPLLLVFASSVWAVYRTGGQ
                     VLPPYVRADAALVLGAAAWDKRPSPVFRERINHAIALYQSRRVGKIVFTGGRTKKGYM
                     TEAEVGRRYALKQGVPARNILFENTSRNTYENLNNIRPVLRANGIASVVIVSDPYHLA
                     RAAEMAEDLGVRVYMSATPTTRFDAGNKKKIFMLQEGYALSLYRLEKWGSRFLGWLSD
                     "
     gene            5118..6512
                     /locus_tag="WGQ68_001079"
     CDS             5118..6512
                     /locus_tag="WGQ68_001079"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEZ0714894.1"
                     /translation="MTVKTRFAPSPTGYLHIGGVRTALFSWAFARHHKGEFLLRIEDT
                     DLARSTAESVNIILDGMKWVGLNYDNADNVVYQTRRFDRYKEVIAELLEKGHAYYCYC
                     SKEELEAMREKAEKEGTATYDRRWRPEAGKTLPEIPAGVQPVVRFKTPLDGVTRWADL
                     VKGEISIPNEALDDLIIARSDGSPTYNFCVVVDDYDMGVTHVIRGDDHVNNTPKQINI
                     LKAIGATLPEYGHLPMILNEQGKKISKRSGDTVAITDFGAMGILPEAMLNYLARLGWA
                     HGDDEFFTMEQFIEWFDLKDVSPSPSRMDLKKLYWINGEHIKITPNGKLAELVKPRLA
                     LRDIHETEKPALEDVLALVKDRAQDLNTLADECLYFYVKQTPTEADVQKHWDDEAAAR
                     MLRFAERLEGLEDWNAEAIHDFFKPFCDEEGIKMGKLGMPLRLAVCGTAKTPSVDAVL
                     ALIGKEEVLKRIRA"
     gene            6533..6928
                     /locus_tag="WGQ68_001080"
     CDS             6533..6928
                     /locus_tag="WGQ68_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714895.1"
                     /translation="MPSDVGIRLQTASKRKLNMKKILYFLMVVFSTSVWAGDAEDNLL
                     SIQSGYRALLQKQNNLDGKIIGMQSDLEDARRRLQAAQADIARLEAEIPAAMAQKARQ
                     AEELRQIGVRLDHAWNAVYGAGGTKASGN"
     gene            6932..7423
                     /locus_tag="WGQ68_001081"
     CDS             6932..7423
                     /locus_tag="WGQ68_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0714896.1"
                     /translation="MNSLFVDNTVFITRLKAGHIGRLVQALFEEWHGFEPWSSVDKIR
                     AYYGRCLKDDELPLAFAAVDDFGTLLGSAAVKRHDMKSFPQYEYWLGDVFVLPEYRGK
                     GIGRRLVAHCIGAARSLGIKFLYLYTPDVQIFYESFGWAVVGRHFHNGEWVTVMRLDM
                     DKV"
     gene            7468..7578
                     /locus_tag="WGQ68_001082"
                     /pseudo
     CDS             7468..7578
                     /locus_tag="WGQ68_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(7652..10246)
                     /gene="mutS"
                     /locus_tag="WGQ68_001083"
     CDS             complement(7652..10246)
                     /gene="mutS"
                     /locus_tag="WGQ68_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEZ0714897.1"
                     /translation="MSKSAVSPMMQQYLGIKAQHTDKLVFYRMGDFYEMFFDDAVEAA
                     KLLDITLTTRGQVDGEPVKMAGVPFHAAEQYLARLVKLGKSVAICEQVGEVGAGKGPV
                     ERKVVRIVTPGTLTDSALLEDKETNRIVAVSPDKKYIGLAWASLQSGEFKTKLTTVDK
                     LDDELARLQAAEILLPDSKNTPQLQTASGVTRLNAWQFAADAGEKLLTEYFGCQDLRG
                     FGLDGKEHAVAIGAAGALLNYIRLTQNLMPQHLDGLSLETDSQYIGMDAATRRNLEIT
                     QTLSGKKSPTLMSTLDLCATHMGSRLLALWLHHPLRSRAHIRARQEAVAALESQYKPL
                     QCRLKNIADIERIAARIAVGNARPRDLAALRDSLFALSEIDLSANGSSLLETLKAVFP
                     ETLPVAETLKVAVMPEPAVWLKDGNVINHGFHPELDELRRIQNHGDEFLLDLEAKERE
                     RTGLSTLKVEFNRVHGFYIELSKTQAEQAPADYQRRQTLKNAERFITPELKAFEDKVL
                     TAQEQALALEKQLFDGVLKNLQTALPQLQKAAKAAAALDVLSTFSALAKERNFVRPKF
                     ADYPAIHIENGRHPVVEQQVRHFTANHTDLDHKHRLMLLTGPNMGGKSTYMRQVALIV
                     LLAHTGCFVPADAATIGPIDQIFTRIGASDDLASNRSTFMVEMSETAYILHHATEQSL
                     VLMDEVGRGTSTFDGLALAHAVAEHLLQKNKSFSLFATHYFELTYLPEAHAAALNMHL
                     SALEQGRDIVFLHQIQPGPAGKSYGIAVAKLAGLPVRALKSAQKHLNELENQAAANRP
                     QLDIFSTMPSEKGDELNVGNFVDKAEEKHFEGILATALENLDPDRLTPREALSELYRL
                     KDLCKSVS"
     gene            10688..11692
                     /gene="gap"
                     /locus_tag="WGQ68_001084"
     CDS             10688..11692
                     /gene="gap"
                     /locus_tag="WGQ68_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003766473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0714898.1"
                     /translation="MSIKVAINGFGRIGRLALRQIEKAHDIEVVAVNDLTPAEMLLHL
                     FKYDSTQGRFQGTAELKDDAIVVNGKEIKVFANPNPEELPWGELGVDVVLECTGFFTN
                     KTKAEAHIRAGARKVVISAPGGNDVKTVVYGVNQDILDGSETVISAASCTTNCLAPMA
                     AVLQKEFGVVEGLMTTIHAYTGDQNTLDAPHRKGDLRRARAAALNIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPVATGSLTELVSVLERPVTKEEINAAMKAAASESYGYNEDQ
                     IVSSDVVGIEYGSLFDATQTRVMTVGGKQLVKTVAWYDNEMSYTCQLVRTLEYFAGKI
                     "
     gene            complement(11762..11881)
                     /locus_tag="WGQ68_001085"
                     /pseudo
     CDS             complement(11762..11881)
                     /locus_tag="WGQ68_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11950..12630)
                     /gene="tsaA"
                     /locus_tag="WGQ68_001086"
     CDS             complement(11950..12630)
                     /gene="tsaA"
                     /locus_tag="WGQ68_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HEZ0714899.1"
                     /translation="MTYTITPIGTARSPYKQKFGIARQPGLASAAEVCIELNPEFTAD
                     SVRGLEDFDYVWISFIFHGVLDEGWAQMVRPPRLGGKQKMGVFATRSPHRPNHLGLSL
                     LKLERIETGKPVRLYCSGADLLDSTPIIDIKPYIPFVESKPDASSGFVSGKPEELEVV
                     WLENIGAENLSESTQNLISQSIAQDPRPAYQDIPERIYVMNIADYEVRFQIEENRATV
                     IDLSPTPL"
     gene            12745..13383
                     /locus_tag="WGQ68_001087"
     CDS             12745..13383
                     /locus_tag="WGQ68_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamidase"
                     /protein_id="HEZ0714900.1"
                     /translation="MIVSIDVDAQKTFTPLCPKELPVNEGHLIVEELNAQAALADLRV
                     MTKDAHHAAAKWLVDNPVDMLKPTGLPDADLTWVAHAMVGTRGYELLDGLPSAKEYDY
                     CVWKGVDPELHPYGACFHDIEEKLSTGLIEWLRCQNTDTVIVGGLATDYCVKTTVLQL
                     LKGGNWQVIVNEAACRGIAPDTIETAWQEMRSAGAVILKNAEEIKKKHINNQ"
     gene            13421..14389
                     /gene="waaC"
                     /locus_tag="WGQ68_001088"
     CDS             13421..14389
                     /gene="waaC"
                     /locus_tag="WGQ68_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase I"
                     /protein_id="HEZ0714901.1"
                     /translation="MKILLVRLSSMGDLIHTLPAIEDLARQCPGVELHWLCEAGFTDI
                     ARLHPFVKKIHVMKWRQWRKHLFQAETWREMGHLKQTLRQEVFDFVLDSQGLIKSACF
                     AKMAKSPICGLDKNSAREGWAALAYVKTYAVPKGKNAVWRNRELFAQVFGYVMPETQV
                     FGLTVPEAGRLKNLAQPYYAALHATSRDSKLWPVENWRSLLQKLNEEQQCNIYLPWGN
                     EDEKTRAKQIADGLPFAIVCDKINLLQAAYLLKYAVGIVGVDTGLLHLANALEKPVVG
                     IYTDTDPIKTGVQVSPIAKNLGNIGQIPTADLVYQALMDCVAADKG"
     gene            14616..15365
                     /locus_tag="WGQ68_001089"
     CDS             14616..15365
                     /locus_tag="WGQ68_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEZ0714902.1"
                     /translation="MKALVAVKRVVDYNVKVRVKADGSDVDIGNVKMSMNPFDEIAVE
                     EAVRLKEAGKVSEIVAVSLGEKKCEETLRTALAMGADRAIHVETDAKLEPLAVAKLLK
                     AVADKENPQIFFLGKQAIDDDANQVAQMLAALLNAAQGTFASKVQIEGDEVQIVREID
                     GGEETIALKLPAVISADLRLNEPRFVKLPNIMAAKKKPLEKLAPADLVADISPRLKTV
                     KFAEPKARQAGVKVASVAELVEKLKNEAKVI"
     gene            15376..16311
                     /locus_tag="WGQ68_001090"
     CDS             15376..16311
                     /locus_tag="WGQ68_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEZ0714903.1"
                     /translation="MSVLIIAEHDNKQLNPATLHAVTAAAKLGKVDLLVAGNGASSVV
                     EFAKQVAGVEKVLVADAAHYAEGLAEELAPLVVKLAADYRYVAATATTFGKNLLPRVA
                     ALLDVPQISDLTEIVDNTTFVRPIYAGNAFETVQANAEKLVLTFRATAFDAAAVQGGN
                     AEVINIETTPAQNLSRFVNRQLSHSDRPELTQAKVIVSGGRALGSAEKFNEVLTPLAD
                     VLGAAIGASRAAVDAEYAPNDAQVGQTGKVVAPQLYFAIGISGAIQHVAGMQDSKVIV
                     AINKDADAPIFNVADYGLVGDLFEIVPQLTEALKN"
     gene            16374..16985
                     /locus_tag="WGQ68_001091"
     CDS             16374..16985
                     /locus_tag="WGQ68_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEZ0714904.1"
                     /translation="MITTYKTITSPTQAGFKDKGSRFIAFAYPIRTLADVKKYLDPLK
                     EEHHKARHWCYAYRLGVDGVQFRANDDGEPSGSAGRPILGQIDSVGITDVLVVVVRYF
                     GGTLLGVSGLIHAYKEATAQALAIAEVIEKNIEKTVWLKCEYPFLNEAIRIAKQYQAD
                     ILEQDLQLDCRLTVSLSLANYEVCVAAWKNTRQIEVNTEKPFE"
     gene            complement(17037..17354)
                     /locus_tag="WGQ68_001092"
     CDS             complement(17037..17354)
                     /locus_tag="WGQ68_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4298 domain-containing protein"
                     /protein_id="HEZ0714905.1"
                     /translation="MQKRIDEIQSKYREWCHLLPQLEEDIRRWKYVVTLIRDMDNFYT
                     HEYQACHQAIEDGVELDLRTEGEYSIMSEDALWNALGEFHQLAWLYLRSSVDALDKYT
                     QED"
     gene            17512..18783
                     /gene="purD"
                     /locus_tag="WGQ68_001093"
     CDS             17512..18783
                     /gene="purD"
                     /locus_tag="WGQ68_001093"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEZ0714906.1"
                     /translation="MKLLVIGNGGREHALAWKLAQSPKVETVFVAPGNAGTAIEPKLQ
                     NIDLTAHQDLIEFSRKENIVFTVVGPEAPLAAGVVDDFRAAGLKIFGPTQYAAQLESS
                     KDFAKAFMAKYNIPTAQYQTFENADAAHDYVNQKGAPIVIKADGLAAGKGVIVAMTLD
                     EAHAAIDDMLLDNKMGNAGARVVIEDFLQGEEASFIVMVDGNNVLPMATSQDHKRLLD
                     GDKGLNTGGMGAYSPAPVVTPVVYERAMNEIILPTVAGMKAEGHEFTGFLYAGLMIDQ
                     SGAPYTIEFNCRFGDPETQPIMSRLNSDLSDLVEAAIDGRLDSVTAEWSPQTAVGVVL
                     AAQNYPETPKKGDVISGLDAANQVGKVFHAGTTANEKGDVLTNGGRVLCVVGLGDNVA
                     QAKAKAYGALEKISFDGMQYRKDIADKAINR"
     gene            18915..19220
                     /locus_tag="WGQ68_001094"
                     /pseudo
     CDS             18915..19220
                     /locus_tag="WGQ68_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            19374..19776
                     /locus_tag="WGQ68_001095"
                     /pseudo
     CDS             19374..19776
                     /locus_tag="WGQ68_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4811 a         4561 c         5490 g         4914 t
ORIGIN      
        1 atcaattgtt tctgtatcgc aaacactgat tgaaaaggac tgattatggc atttttaaaa
       61 ctgaccgaac aaaacgtgca gggcaaaacc gtcctcatcc gcgccgatat gaacgtgccg
      121 ttcaaagacg gcaaaatcag cgacgacacc cgtatccgcg cttcgctcgc gtccgtcaaa
      181 tactgcttgg acaacggcgc gtccgttatc gtgatgaccc acttgggccg cccgactgaa
      241 ggcgagttcc accccgaaga cgacgttgcg cccgttgccg cgcatttggg cggtctgttg
      301 ggcaaagacg tgaaagtatt gaacgactgg cgcgaaaaca aaccggcttt gaacgcgggc
      361 gatgtcgtga tgctgcaaaa cgtgcgtatc aacaaaggcg agaagaaaaa cgatttggaa
      421 ctgggcaaag cctacgcttc tttgtgcgac gtgttcgtca atgacgcgtt cggcaccgcc
      481 caccgcgccc aagcctcgac cgaagccgtc gcccaagccg cgcccgttgc ctgcgccggc
      541 gtattgatgg cgggcgaact cgacgctttg ggcaaagcct tgaaacagcc tgcacgcccg
      601 atggttgcca tcgttgccgg cagcaaagtg tccaccaagc tgaccatcct cgaatcgctg
      661 gcggacaaag tcgaccaact catcgtcggc ggcggtatcg ccaacacctt cctgttggcg
      721 gaaggtaaag ccatcggcaa atctttggcg gaacacgatt tggtggaaga atccaaaaaa
      781 attatggcaa aaatggcggc caaaggcggt tctgtgccgc tgccgaccga tgtcgtcgtt
      841 gccaaagcct ttgccgccga tgccgaagcg gtggtaaaag acattgccga cgttgccgaa
      901 gacgagatga ttttggacat cggcccgaaa tctgccgccg cgcttgccga tttgcttaaa
      961 gccgccgaca cggtcgtttg gaacggaccg gtcggcgtgt tcgagtttga ccagttcgca
     1021 ggcggtacga aagcccttac cgaagccatt gcccaaagca aagcgttctc cattgctggc
     1081 ggcggcgaca cgctggcggc gattgccaaa ttcggcgtta ccgaccaaat cggctacatc
     1141 tccaccggag gcggcgcgtt ccttgagttc ttggaaggta aagagttgcc tgccgtagcc
     1201 gctttggaaa aacgcggcga gtaaacggtt tgatataaaa aaatgccgtc tgaaacgttg
     1261 tgctgtttca gacggcattt ttgtgtggcg gttattgtgc tttggcattc cattctgccg
     1321 gtgtggcggc taccgaaatg gaaagtgcgt gcagttcgtt gctttccagt tgggctttaa
     1381 gcccgtcttt aatcaggcgg tggcgcgcga ggcgtgcctt gccttcaaat tctgatgaaa
     1441 cgatgacggc gaaaaaatga tgtccgtcgc cttctacttc gatgtgttcg cattttgcca
     1501 cgccttcaat catggctttt acttgttccg aggtcagcat ggtaggttcc ttgttggaaa
     1561 aatgcgccat tatactggca aattgaaatg ccgtctgaaa acgggttcgg acggcatttt
     1621 tgacggtgtt ttattttttg gctttgaagc cgagcaggtg ctgggtggtg gcaagccacg
     1681 cgccgaatat ccccagtgcg atgacgaagc cgaacaccag tgcgatttct cccgggtaga
     1741 agaaacgcca gccgatatta agtccgtagg gtttgaagat ggcatcgacc aaggggcgca
     1801 cggcagagag cagccaaccg caaagcccca agctgacggc ggcggaaagg atgctctgcc
     1861 acatggcttg ataaaggaat gggcggcgga taaacgacgc gggcgcgccc aagagtttgg
     1921 tgatttcgat ttcttctttg cggctgagga tttgcagtcg gatggtgttg tgcgcgacaa
     1981 ggacgaacgc catccccagc gtcagggaaa gaaaccataa gattttacgg atgaattcgt
     2041 tgatttggta cagtgtctgt acccattccg tatccatgtt ggcggattcg accataggca
     2101 gtttggtaat gtcccggtag attgcctgca tttgggcggg cgtggttgcc gggtcggggg
     2161 taacgataaa gacatccggc agggggttac cgtcaagcat ggaaatcaga ttttggtcga
     2221 gattggactg taattcttcc aaaccgtctt ctttgctgat aaagcggatg ttgtcgagcc
     2281 gtttgtcgcg caccagcagg ctgcggacgg tatcgctgtc gctttgtgcg gcggcggttt
     2341 ccatatagac ggtgatttgc ggcgactcat tgagtttgcc caacacgctt tgcccgcttt
     2401 ggatgcccaa gtacataaac agcggcaggg tcatggcgac ggcgagcatt atgagggtaa
     2461 gcagtgtgcc gaaaggttgg cgcagaagct gtttgagcgc ggtgcgcgcg gattcgacgt
     2521 gcagcgagag gtagtggatg atgctcatgc gagtcgtcct ttcgagaggc gcaggatgcg
     2581 gtgtccgtag tccgccatca gggtttcgtc atgtgcggcg acgatgacgg ttgttcccgc
     2641 ttcgtggaag gttttgaaca attccataat atcgagcgcg taggcgcggt cgaggttggc
     2701 ggagggttcg tcggcaatca gcaggctggg ctggtgaacg acggcgcggg cgatgcacag
     2761 gcgttgttgt tcaccgccgg agagggttac gggatcgtcc aattctcgtc ctttcaggcc
     2821 gactttttcg atggcgatgc gggcacgctc ttcggctttg cgcggcggat agccgataat
     2881 ccgaagcggc aggatgacgt tttgcaggac gttgcggtcg tagaggattt tgtggtcttg
     2941 gaacacgatg ccgatatgtt ggcgcataaa gccgatttgg ttgtcggaca atgtgccgag
     3001 atcctgcccg ttaaacagga ttttgcccct gctcggcttg gtgatgcccg aaatcagctt
     3061 gaggacggtg gatttgcccg aaccggagtg tcccgcgata aatatcattt cgcctttgtt
     3121 gatttggaag ctgacgtttt tcagggcttc aaaaccgccg ggataggttt tggaaacttg
     3181 ttcgaaacgg atcataggag gtcctgtaag gaagggcggc atatcgtttc gggcggttgc
     3241 gtccgcaggg atatgccgtc tgaacaatag gtaagacagt gccgattata aagattccgc
     3301 ctgtttttag atagtggata agtttggcgg ataccgaagg tcgggcggga aaaatgccgt
     3361 ctgaagcggc ttcagacggc atccggcgtt taacggcatt ttgaatgggc gagtttgacg
     3421 gcttcggtca ggcttttttc gttcacttcc ccggtaatgg tttgtttgta cccgcatttc
     3481 ggggcttcga cgacggtaaa gggcagtacg ccgacattgt ttccgtagga tttcataaag
     3541 tttcggctgt tcgcctcggt gtaacgccaa atcgggtagg aaacaggagt ttgtttgagg
     3601 aagttgccga tattgtcgga tgtgtcgagc gcgatgccga ccatatcgac gctgcctttt
     3661 ttctgcgctt tgtaccattt ggacatggcg ggcatctctt ttcggcacgg gccgcaccag
     3721 gttgcccaaa ggttgacgat gcgtacgggg gctttgagtg attgcaggct ttgtggggtg
     3781 ttgtctttcc atccggccag ttcgtctgcc gaggcggtat gtgcggcaaa tgtggcggca
     3841 agggtgattg cggcaagatt caagtatttc atcgttttgt cctttgtatg tcgattcggg
     3901 tattgtaaac cgtttggtac ggttgtaggc gggatattga gtaaaaccct gtgtaagccg
     3961 ataataaggg gattgtcgag aagcaaggaa atgtcatgcc tgaaatcaaa attttccaca
     4021 atccgcgttg cagcaaatcg cgtgccgcct tgtccctatt ggaagaacgc ggcattgctg
     4081 ccgaagtggt caaatatttg gatacgccgc ccgacttgtc cgaattgaag gatattttca
     4141 acaaattggg cttggcatcg gcgcgcggga tgatgcgcgt gaaagatgat ttgtataagg
     4201 aattgggttt ggacaacccc aatttggaca atgacgcgct gctgcgtgcc atcgccgatc
     4261 atcccgccct gttggagcgt ccgattgttt tggcaaacgg taaggcggct gtcggcagac
     4321 cgctggaaaa tatcgaagcg gtattgtgaa caaacggctg ttttgcagcc gcaacggctt
     4381 gcgctattac ctgttgggcg gtttctgttt gagtgttttt ccgcttttgc tggtgtttgc
     4441 ctcatccgtt tgggcggttt atcggacggg cgggcaggtt ttgccgccgt atgtccgggc
     4501 ggatgccgcg ctggtgttgg gcgccgccgc gtgggacaaa cgcccttccc cggttttccg
     4561 cgaacgcatc aaccacgcca tcgcgcttta tcagagccgc agggtaggca aaatcgtgtt
     4621 tacgggagga cgaaccaaaa aaggctatat gaccgaagcc gaggtcgggc ggcggtacgc
     4681 gctcaaacag ggggttccgg cgcgcaacat cctgtttgaa aacacttcgc gcaacactta
     4741 tgaaaacctg aacaacatcc gtccggtttt gcgtgccaac ggtattgcca gcgtggtgat
     4801 tgtcagcgat ccctaccatt tggcgcgggc ggcggaaatg gcggaggatt tgggtgtccg
     4861 cgtttatatg tctgccaccc cgaccacgcg gtttgatgcg ggaaataaga aaaaaatctt
     4921 tatgctgcaa gagggatatg cgttatccct ctaccgtttg gaaaaatggg gcagccgctt
     4981 tttgggatgg ctgtctgatt gaagcggtag aaaatgccgt ctgaaactta tccgcaggca
     5041 gggttcggac ggcatttttg atgcccaagc acggtaaaac cagtaaaata aacgctttca
     5101 aagatttcgg agccatcatg accgtcaaaa cccgtttcgc ccccagcccc accggctacc
     5161 tgcacattgg cggcgttcgc accgccttgt tttcgtgggc gtttgcccgc catcataaag
     5221 gcgaattttt attgcgcatc gaagacaccg acttggcgcg ttctaccgcc gaatccgtca
     5281 acatcatcct cgacggcatg aaatgggtcg gcctgaacta cgacaacgcc gacaatgtcg
     5341 tgtaccaaac ccgccgtttc gaccgctaca aagaagtcat tgccgaactt ttagaaaaag
     5401 gccatgccta ctactgctat tgcagcaaag aagagctgga agcgatgcgc gagaaagccg
     5461 aaaaagaagg cacggcgact tacgaccgcc gctggcggcc ggaagcgggc aaaaccctgc
     5521 ccgaaattcc cgccggcgtg caacccgtcg tccgcttcaa aacgcctttg gacggtgtaa
     5581 cccggtgggc ggacttggtc aaaggcgaaa tttccatccc caatgaagcc ctcgacgacc
     5641 tgattatcgc ccgatccgac ggctcgccga cctacaactt ctgcgtcgtg gtggacgact
     5701 acgacatggg cgttacccac gttatccggg gcgacgacca tgtgaacaac accccgaaac
     5761 aaatcaacat attaaaagcc atcggcgcta ccctgcctga atacggccac ttgcccatga
     5821 ttcttaacga acaaggcaaa aaaatctcca aacgcagcgg cgataccgtt gccattaccg
     5881 atttcggcgc aatgggcatc ctgcccgaag ccatgctcaa ctatctggca cgcttgggct
     5941 gggcgcacgg cgacgacgag ttctttacga tggaacaatt catcgaatgg tttgatttga
     6001 aagacgtttc cccgtcccca agccgtatgg atttgaaaaa gctctattgg atcaacggcg
     6061 aacacatcaa aatcacgccc aacggcaaac tcgccgaact cgtcaaaccg cgtctcgctc
     6121 tgcgcgatat ccatgaaact gaaaaacctg cattggaaga cgtgttggca ctggtcaaag
     6181 accgcgccca agacctgaac acacttgccg acgagtgcct ctacttctac gtcaaacaaa
     6241 cgcctactga agcggacgtg cagaaacact gggacgatga agccgccgcc cgtatgctgc
     6301 gctttgccga acgcctcgaa gggctggaag actggaatgc cgaagccatc cacgacttct
     6361 tcaaaccctt ctgcgacgaa gaaggcatta aaatgggcaa actcggcatg cccctgcgcc
     6421 ttgccgtctg cggtacggcg aaaaccccca gcgtcgatgc tgtgttggca ttaatcggca
     6481 aagaagaagt gttgaaacgc atccgcgctt aagttatcca caggtgcagg cgatgccgtc
     6541 tgatgtcgga atacggcttc agacggcatc caaacggaag ttgaacatga aaaaaatatt
     6601 gtattttctg atggttgttt tttctacaag cgtatgggcc ggggatgcgg aagacaatct
     6661 gctcagcatc caatccggct atcgtgcctt actgcaaaag caaaacaatc tggacggaaa
     6721 aatcatcggg atgcagtcgg atttggaaga tgcgcgcaga cggttgcagg cggctcaggc
     6781 agacatcgcc cgtttggaag cggaaattcc tgcagcaatg gcgcaaaaag cccggcaggc
     6841 tgaagagctg aggcaaatcg gagtgcgttt ggaccatgct tggaatgcgg tttacggcgc
     6901 agggggaacg aaggcgtcgg ggaattgacc gatgaattcc ctctttgtgg acaatactgt
     6961 tttcattaca cggctgaaag ccgggcatat cggcaggttg gttcaggcgt tgtttgagga
     7021 gtggcacgga tttgaaccgt ggtcttctgt ggataagatt cgtgcctatt acggcagatg
     7081 tttgaaagat gacgaactgc cgctggcttt tgcggctgtg gatgatttcg gaaccctgtt
     7141 gggttcggct gcggtcaagc ggcatgatat gaaaagtttc ccacagtatg aatattggtt
     7201 gggggatgtc tttgttttac ctgaatatcg cggaaaaggc attggcagga ggctggtcgc
     7261 ccattgcata ggcgcagcgc gttcgctggg gataaagttc ttgtatcttt atacgcctga
     7321 tgtgcaaata ttttatgaat cattcggctg ggcggttgtc gggcgacatt tccataacgg
     7381 tgaatgggtt acggttatgc gtttggatat ggataaggtt taagccgaat ggacaaatgc
     7441 cgtctgagct gttcggacgg cattttttat agtggattaa atttaaatca ggacaaggcg
     7501 acgaagccgc agacagtaca aatagtacgg caaggcgagg caacgccgta ctggtttaaa
     7561 tttaatccac tatatgtttg gaaatttcct gtcagataat gtttatccac agatttttcc
     7621 atatggtttg atgctgttcc gacaacggaa attaagatac ggatttgcac aaatctttca
     7681 gacggtacag ttctgacaat gcttcgcgcg gagtcagtct gtcgggatcg agattttcca
     7741 aggctgttgc cagtatacct tcaaaatgtt tttcctctgc tttgtccaca aagttgccca
     7801 cattcagttc atctcctttt tcagacggca tggtactgaa aatatccagt tggggacggt
     7861 tcgcggcggc ttggttttcc agttcgttca aatgcttttg ggcggatttc aatgcgcgta
     7921 caggcagtcc ggcgagtttg gcgacggcga tgccgtagct tttgccggcg ggacccggtt
     7981 ggatttggtg caggaacacg atgtcccgtc cctgttcgag cgcggaaagg tgcatattga
     8041 gtgcggcggc gtgggcttcg ggcaggtagg tcagctcgaa atagtgggtg gcaaacaggc
     8101 tgaaggattt gtttttttgc agcaggtgtt cggcaacggc gtgcgcgagg gcgaggccgt
     8161 cgaaagtgga agtaccacgt ccgacttcgt ccattaaaac aaggctttgt tcggtggcgt
     8221 gatgcaggat gtaggcggtt tcgctcattt cgaccatgaa agtggagcgg ttggaggcga
     8281 ggtcgtccga tgcgccgatg cgggtgaaga tttgatcgat gggcccgatt gtggcggcat
     8341 cggcaggcac aaaacagccg gtgtgtgcca ataaaacaat cagcgcgact tggcgcatgt
     8401 aggtggattt gccgcccata ttggggccgg tgagcagcat gaggcggtgt ttgtggtcaa
     8461 ggtcggtgtg gttggcggtg aagtggcgta cctgctgttc gacaacggga tggcggccgt
     8521 tttcgatgtg gatagccgga tagtcggcaa acttggggcg gacgaagttc cgctcttttg
     8581 ccaaggctga aaatgtggac aacacgtcca gcgcggcggc ggctttggcg gctttttgaa
     8641 gctgcggcaa tgccgtctga aggtttttca atacgccgtc aaagagttgt ttttctaagg
     8701 cgagggcttg ctcttgagca gtcagcactt tgtcttcaaa ggctttcaac tccggcgtga
     8761 tgaagcgttc ggcgtttttg agggtttgcc ggcgttggta gtcggcaggt gcttgttcgg
     8821 cttgggtttt ggataattca atgtaaaagc cgtgaacgcg gttgaactcg actttgagtg
     8881 tggacaaacc ggtacgttcg cgttccttgg cttccaaatc cagcaaaaat tcgtcgccat
     8941 ggttttgaat gcggcgcaat tcgtccagtt cgggatgaaa accgtggttg atgacattgc
     9001 cgtctttcag ccagacggca ggttcgggca tcacggcgac tttgagggtt tcggcaactg
     9061 gcagggtttc ggggaaaacg gctttgaggg tttccaacag gctgctgccg tttgcggaca
     9121 agtcgatttc ggacagggca aacaggctgt cgcgcagggc ggcgaggtcg cgcgggcggg
     9181 cgttgccgac ggcgatgcgg gcagcaatgc gttcaatgtc cgcaatgttt ttcagacggc
     9241 attggagggg tttgtattgg ctttccagcg cggcaacggc ttcttggcgc gctcggatgt
     9301 gggcgcggct gcgtaagggg tggtgcaacc agagagccaa gaggcggctg cccatatggg
     9361 tggcgcaaag gtcgagcgtg gacatcaggg tcggcgattt tttgccggag agggtttgcg
     9421 tgatttcaag attgcggcgc gtggcggcat ccataccgat atattggctg tcggtttcga
     9481 gcgacaggcc gtccaaatgt tgcggcatca ggttttgcgt cagacggata tagttcaaca
     9541 gtgcacctgc cgcgccaatc gcaacggcgt gttctttgcc gtccaaaccg aagccgcgca
     9601 aatcctggca gccgaaatat tccgtcagca gtttttcccc cgcgtcggcg gcaaactgcc
     9661 acgcgttcag gcgcgtaaca cccgatgccg tctgaagttg cggtgtgttt ttactgtcag
     9721 gcaacagaat ttccgccgcc tgcaggcgcg ccagttcgtc gtccaattta tccacagttg
     9781 tcagcttggt tttgaattcg ccgctttgca gcgatgccca cgccaaaccg atgtattttt
     9841 tgtcggggga cacggcaacg atgcggttgg tttccttgtc ttccagcaat gcggaatcgg
     9901 tcagcgtgcc gggcgttacg atgcgcacga ctttgcgctc caccggtcct ttgcctgcgc
     9961 cgacttcgcc gacctgttcg caaatcgcca cgcttttgcc caacttgacc aggcgcgcca
    10021 gatattgttc ggcggcgtga aacggcacgc ctgccatttt gaccggctcg ccatccacct
    10081 gtccgcgcgt ggtcagggta atatccaaaa gttttgccgc ttctaccgca tcgtcgaaaa
    10141 acatctcgta aaaatcaccc atgcggtaaa acaccagttt gtcggtatgt tgcgctttga
    10201 tgccgaggta ttgctgcatc attggggaaa cggcggattt ggacatgggg ctgccttatc
    10261 attaggaaaa cggtattgta gcaaaaggtt ttgtcttcga cggaatgaga ctgaccgtaa
    10321 gcctgtcaat aaggttgtgg agacaagtag gaaaacagca aagttatcca cggagatatt
    10381 attaagtcaa aaaacatcca cgtttatctg tcctatacgg caagccttaa actcaaactt
    10441 atttgacaaa aagataatga aaatattgga tatttgctac ttacgcacaa gcaggttggc
    10501 aatcatcaaa atcatcatta aaatttaaat tcaaatattg atggtaaagg ttttggaaat
    10561 ctgtgcaaac agcaaaattc atctatatca aataagcgtc aagtagttag atgtaatagt
    10621 ggaatgtaac tttatttcag aatacaatgc aggcatcgga ctttatttca caacctggag
    10681 acaaaatatg agcattaaag tagcgattaa cggtttcggc cgcatcggcc gcctcgcatt
    10741 gcgtcaaatt gaaaaagccc acgacatcga agtcgtcgcc gtcaacgacc tgacccctgc
    10801 cgaaatgctg ctgcaccttt tcaaatacga cagcacgcaa ggccgtttcc aaggtactgc
    10861 cgaattaaaa gacgatgcca tcgtggtaaa cggcaaagaa atcaaagttt ttgccaatcc
    10921 gaatcccgaa gaattgcctt ggggcgagtt gggtgtggat gtcgtcctcg aatgtaccgg
    10981 cttctttacc aataaaacca aagccgaagc acacatcagg gccggcgcgc gcaaagtcgt
    11041 gatttccgca cccggcggca atgatgtgaa aaccgtcgta tatggcgtaa accaagacat
    11101 tttggacggc agcgaaaccg ttatctccgc tgcttcctgc accaccaact gccttgcccc
    11161 gatggcggca gtcctgcaaa aggaattcgg cgtagtcgaa ggcctgatga ccaccatcca
    11221 cgcctacacc ggcgaccaaa acacccttga cgcgccgcac cgcaaaggcg atttgcgccg
    11281 cgcccgcgcc gccgcgctca acatcgtgcc caacagcacc ggtgccgcca aggccatcgg
    11341 tctggttatc cctgaattga acggcaaact cgacggctcc gcccaacgcg tccctgttgc
    11401 caccggctcg ctgaccgaat tggtttccgt cctcgaacgc cctgtaacca aagaagaaat
    11461 caacgccgcg atgaaagccg ccgccagcga atcttacggc tacaacgaag atcaaattgt
    11521 ttcttccgac gttgtcggca tcgaatacgg ttcgctcttc gatgcgaccc aaacccgcgt
    11581 gatgaccgtg ggcggcaaac aattggtgaa aactgttgcc tggtacgaca acgaaatgtc
    11641 ctacacctgc caactcgtcc gcactttgga atactttgca ggcaaaatct aaggcttgaa
    11701 caaacctgtg gataagtaaa tgccgtctga acacattgcg ttcagacggc attgtttatc
    11761 tgtatagtgg attaaattta aaccagtacg gcgttacctc gccttgccgt actatttgta
    11821 ctgtctgcgg cttcgttgcc ttgtcctgat ttaaatttaa tccgctataa cttgatattg
    11881 cggcttatgg cgaacaaaca acagccgttt gttcaactgc tatgcggcaa aaccggattt
    11941 ttgcccgatt taaagcgggg ttggggaaag atcaataacg gttgcacggt tttcttcgat
    12001 ttgaaaccta acttcgtaat ccgcaatatt catcacataa atccgttcgg gaatatcctg
    12061 ataggcgggg cgcgggtctt gggcaatgct ttggctgatt aggttttggg tgctttcaga
    12121 taaattttcc gcgccgatgt tttccagcca tacaacttca agctcttcgg gtttgccgct
    12181 gacgaagccg gatgatgcat cgggtttgga ttcgacaaag gggatgtagg gttttatgtc
    12241 gataattggt gtgctgtcca acaggtctgc gccgctgcaa tagaggcgga cgggtttgcc
    12301 ggtttcgatg cgttcgagtt tcaggagcga gagtccgaga tggttggggc ggtgggggct
    12361 gcgcgtggcg aacacgccca ttttttgttt gccgccgagc cgtggcgggc gcaccatttg
    12421 cgcccagcct tcatccaata cgccgtgaaa aataaaactt atccacacat aatcgaaatc
    12481 ttccagcccg cgcacgctgt ctgcggtaaa ttcgggattc agctcgatgc atacttctgc
    12541 ggcagaggct aaaccgggct ggcgggcgat gccgaatttc tgtttgtagg gcgatcgggc
    12601 ggtgccgatg ggggtaatgg tataagtcat aaggttgtgg ataagttttt ttggaaaaac
    12661 cttatcttat cacaatcggt tttaccgttt ttgggcagta atttgttata atacagggca
    12721 ttttaatcgc gttgaaataa tgagatgatt gtttccattg atgttgatgc acaaaaaacg
    12781 tttacgcctt tgtgtcctaa agagctgcct gtgaacgagg ggcatttgat tgttgaagag
    12841 ttgaatgccc aagccgcttt ggcggatttg cgcgtgatga cgaaagatgc gcatcatgcg
    12901 gcagcaaaat ggcttgtgga taaccctgtt gatatgttga agccgacagg tttgcctgat
    12961 gcggatttga cttgggtggc tcatgcgatg gtcggtacgc gcggctatga attattggac
    13021 ggactgcctt ctgccaaaga atacgattat tgcgtttgga aaggtgttga tcctgaattg
    13081 catccttacg gcgcgtgttt tcacgatatt gaggaaaaac tgagcacagg gctgattgaa
    13141 tggctgcgtt gtcaaaatac ggatacggtc attgtcggcg gcttggctac ggattattgt
    13201 gtgaaaacaa cggttttgca gttgcttaaa ggtggtaatt ggcaggttat cgtcaatgaa
    13261 gcggcttgtc ggggtattgc gccggatacc atcgaaacgg catggcagga aatgcgttct
    13321 gccggtgctg tcatcttaaa aaacgccgaa gaaattaaaa aaaaacacat taataatcaa
    13381 taagttaaaa ttgtttcacg tgaaacatac ttcttaaaat atgaaaattt tgcttgtccg
    13441 tttgtctagt atgggcgatt taattcacac tttgcccgca attgaggatt tggcgcgaca
    13501 atgccctggt gtagaactcc attggctgtg tgaagctgga tttacagata ttgcgcgcct
    13561 gcatccgttt gtgaaaaaaa tccatgtgat gaaatggcgg caatggcgca aacatctctt
    13621 tcaggctgaa acttggcggg aaatgggtca tctgaaacag actttgcggc aggaagtatt
    13681 tgatttcgta ttggacagtc aaggtctgat taaaagcgcg tgtttcgcta aaatggcaaa
    13741 atccccgatt tgtggtttgg ataaaaacag tgcgcgcgag ggatgggctg ctttggcgta
    13801 tgtaaaaaca tacgctgtac cgaagggaaa aaatgccgtt tggcgcaacc gtgaactgtt
    13861 tgcccaagta tttgggtatg taatgccgga aacgcaggta tttggcttga ctgttcctga
    13921 agcaggccgt ctgaaaaatt tagcgcagcc gtattatgcg gctttgcatg cgaccagtcg
    13981 ggacagtaag ttatggcctg tggaaaactg gcggtcattg ctgcaaaagt tgaatgaaga
    14041 acagcaatgc aatatttacc tgccttgggg aaatgaagat gaaaaaacgc gtgccaaaca
    14101 aattgcagac ggactgccgt ttgctattgt gtgcgacaaa ataaatttat tgcaggcagc
    14161 gtatctgctg aaatacgcgg tcggaattgt cggcgtggat accggtttgc tgcatttggc
    14221 aaatgccttg gaaaaacctg tggtcggcat ttataccgat accgatccga ttaaaacagg
    14281 cgttcaggtt tcgccaattg caaaaaattt gggcaatatc gggcagattc cgaccgcaga
    14341 tttggtttat caagcgctga tggattgtgt tgcagcagat aaaggctaaa gggtcgtcta
    14401 aaactgatat ggcggcatga tattttccaa tttattagat acaggaatgt atatccgcta
    14461 gtctgagtaa atgctttaaa aatggtataa tcggcaagca ttatccgaaa gatttttgaa
    14521 tgaattcgtt tttaaggaaa acggatttgg ttttataggg aagaaggttt ggtttcgctg
    14581 gatggtgaaa gcagcgttct ttttaggagg gttcaatgaa agcactggtc gcagtaaagc
    14641 gcgtagtgga ctacaacgtc aaagttcgtg taaaagccga tggttcggat gtggatatcg
    14701 gcaatgtcaa aatgtcgatg aatccgtttg acgaaatcgc tgtggaagaa gccgtccgtt
    14761 tgaaagaagc cggaaaagta agcgagatcg tagcggtttc tttgggcgaa aagaaatgcg
    14821 aagaaacctt gcgtacagct ttggcgatgg gtgccgaccg tgcaattcat gttgaaaccg
    14881 atgcgaaatt ggagccgctg gcagttgcca agttgctgaa agccgttgcg gacaaagaaa
    14941 atccgcaaat tttctttttg ggtaaacaag cgattgatga cgatgctaat caagtggcgc
    15001 aaatgctggc agctttgttg aatgcggcgc aaggtacgtt tgcttcaaaa gtacaaattg
    15061 aaggtgatga agtacagatt gtgcgcgaaa ttgatggcgg cgaagaaacc atagcattga
    15121 aattgcccgc tgttatcagt gcagatttgc gtttgaacga gccgcgcttc gtcaaactcc
    15181 ccaatattat ggcggcgaag aaaaaacctt tggaaaaact ggctcctgcc gatttggttg
    15241 ctgacatttc acctcgtctg aaaacggtga aattcgccga gcctaaagcg cgtcaggcag
    15301 gcgtaaaagt agcaagcgtt gccgaattag ttgaaaaatt gaaaaacgaa gccaaagtga
    15361 tttgaggaga ctgaaatgag cgtattgatt attgccgaac acgacaacaa acagttgaat
    15421 cctgccactt tgcatgctgt taccgctgcc gcaaaactag gcaaagtcga tttattggtt
    15481 gccggaaacg gcgcatcgtc cgtcgtggag ttcgcgaagc aagtagcggg agtggaaaaa
    15541 gttttggttg cagatgctgc ccattatgct gaaggtttgg ctgaagagtt ggctccgctg
    15601 gttgtcaaat tggcggcgga ttaccgctat gttgcagcaa cggcaaccac attcggtaaa
    15661 aaccttttgc cccgcgtagc cgccttatta gacgtaccgc aaatttctga tttgaccgag
    15721 attgtagata acacgacttt tgtacgtccg atttatgcag gtaatgcatt tgaaaccgtg
    15781 caagccaatg cggaaaaatt ggtgctgacc ttccgtgcga cggcttttga cgcagcggcg
    15841 gtgcaaggtg gtaatgctga ggtaattaat attgaaacaa cccctgccca aaacctgagt
    15901 cgttttgtta accgtcagct ttcccattcc gatcgtcccg aactgactca ggcaaaagtg
    15961 attgtttctg gtggtcgtgc gttgggtagt gcggaaaaat ttaatgaagt gctgacaccg
    16021 ttggcggatg ttttaggtgc ggcaatcggt gcatcccgtg cagcagttga tgccgaatat
    16081 gcgccaaacg atgctcaagt cggacaaacc ggtaaagtag ttgcgccgca actctatttt
    16141 gcaatcggta tttcaggtgc gattcaacac gttgccggta tgcaagacag taaggtgatt
    16201 gttgcaatca ataaagatgc tgatgcgccg attttcaatg tagccgatta cggattggtt
    16261 ggcgatttat ttgaaatcgt tccacaatta acagaagcat tgaaaaatta atgtttcacg
    16321 tgaaactttc agacgacttt gttattaatg aggttgtctg aaaacttttc aacatgatta
    16381 cgacttataa aaccattact tccccgacgc aggctgggtt taaagataaa ggcagccgtt
    16441 ttatcgcatt tgcctatccg attcggacat tggctgatgt gaaaaaatac ctcgatccgt
    16501 taaaggaaga gcaccataaa gcacgacact ggtgttatgc ctatcgtttg ggcgtggatg
    16561 gtgtgcaatt tcgtgccaac gatgatggag agccatcagg aagtgctgga cgaccaattt
    16621 tgggacagat tgattcggtg ggcatcaccg atgttttggt cgtggtcgtc cgctatttcg
    16681 gtggtacttt attgggcgtt tcaggtctga tacatgcgta caaagaggcg acggctcagg
    16741 cgttggcgat tgcggaagtg attgaaaaga atattgaaaa aaccgtttgg ctgaaatgtg
    16801 aatatccatt tttgaatgaa gcgatacgca tcgccaaaca atatcaggcg gatatattgg
    16861 agcaggattt gcagttggat tgccgattga cggtaagttt gtcgttggca aattatgaag
    16921 tctgtgttgc ggcttggaag aatacacggc agattgaagt aaatacggaa aagccttttg
    16981 aatgaaaggt cgtctgaaat gctttgtgat ggtatttcag acgacctctt cgctgactaa
    17041 tcttcttgtg tatatttgtc taaggcatcg acgctggagc gcaaatataa ccaagccagt
    17101 tgatggaatt cgcccagcgc gttccatagc gcatcttcac tcataatgct gtattcgcct
    17161 tccgtacgca aatccagttc taccccgtct tcaatagcct gatgacacgc ctgatactcg
    17221 tgggtataga aattgtccat gtcgcgaatt aaagtgacga catatttcca acggcggatg
    17281 tcttcttcca gttgcggtag taaatgacac cattcgcggt atttgctttg gatttcatca
    17341 atacgttttt gcatggttgc ttgtcctttt ttaatttatg gataagttga ataggttgca
    17401 gagtggaatg aagcggtctt ttgtttgatt ctactaaaat ttcttcagat gggataaaag
    17461 tccctttgta tcgagttttc gttaaaagta catatttgtc aaggagatgg tatgaaactg
    17521 ctggttatcg gtaatggcgg tcgcgaacac gcgctggctt ggaaattggc gcagtcgcct
    17581 aaagtggaaa cagtatttgt tgcgcctggt aatgccggta cagcgattga acccaagttg
    17641 caaaacatcg acttgactgc gcatcaggat ttgattgaat tcagtcgtaa agaaaatatt
    17701 gtttttaccg tcgtcggccc tgaagcgcct ttggcggcag gtgttgtgga tgatttccgt
    17761 gccgcaggac taaaaatatt cggtccgaca caatatgcgg cgcagttgga aagttccaaa
    17821 gatttcgcca aagcatttat ggcgaaatac aacattccga ccgcgcaata tcaaaccttt
    17881 gaaaacgccg atgccgcaca tgattacgtc aatcagaaag gtgcgcctat cgttatcaaa
    17941 gccgatggtt tggcggcagg taaaggcgtg attgtggcga tgactttgga tgaagcgcat
    18001 gctgcgattg atgatatgct gctggacaac aaaatgggca atgccggcgc gcgtgttgtg
    18061 attgaagatt tcctgcaagg cgaagaagcg agttttatcg tcatggttga tggcaataat
    18121 gtactgccta tggcaaccag ccaagaccac aagcgtcttt tagacggcga caaaggtctg
    18181 aatacgggcg gtatgggtgc gtacagccct gcgcctgtgg taacgcctgt tgtgtacgag
    18241 cgcgcgatga atgaaattat tttgccgacc gtagcaggga tgaaagcgga ggggcatgag
    18301 ttcaccggtt tcctgtacgc aggtttgatg attgatcaaa gcggtgcacc ctatacgatt
    18361 gagtttaact gccgtttcgg cgatcctgaa acccagccga ttatgagccg actgaacagc
    18421 gacttatcgg atttggttga agcggcaata gacggcagac tcgatagcgt aaccgcggaa
    18481 tggagcccgc aaactgccgt gggcgtggta ctggcggcgc aaaattaccc cgaaacgccc
    18541 aaaaaaggcg atgttatttc cggtctggat gctgccaacc aagtaggcaa agttttccat
    18601 gcaggtacaa cggcaaacga gaaaggtgac gtattgacca acggcggacg cgtattgtgt
    18661 gttgtgggat tgggcgacaa tgtggcgcaa gcgaaagcta aggcatatgg cgcgttggaa
    18721 aaaatcagtt tcgacgggat gcaataccgt aaagacattg ccgataaagc gattaaccgt
    18781 taactagaaa gaggatgatg ccgtctgaag ccaagtatga ctgatgtgct tcagacggta
    18841 ttttatatta tgagaccttt gcaaaattcc ccaaaatccc ctaaattccc accaagacat
    18901 ttaggagatt tctcatgagc accttcttcc agcaaaccgc ccaagccatg attgccaaac
    18961 acatcgaccg tttcccgcta ttgaagttgg accaagtgat tgattggcag ccgatcgaac
    19021 catacctgaa ccgtcaaaga acccgttacc tccgagacca ccgcggtcgt cccgcctatc
    19081 ccctgttgtc catgttcaaa gccgtcctgc tcggacaatg gcacagcctc tccgatcccg
    19141 aactcgaaca cagcctcatc acccgcatcg acttcaacct gttttgccgc tttgacgaac
    19201 tgagcatccc tgattacagt atagtggatt aacaaaaacc agtacggcgt tgcctcgcct
    19261 tagcttaaag agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta
    19321 tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaatccac tatactttat
    19381 gccgctaccg caactggctg gcgcaagacg acaccctgtc cgaattgctg gaactgatta
    19441 accgacaact gaccgaaaaa aacctaaaag tagagaaagc atccgccgcc gtcattgaca
    19501 ccaccattat tcagaccgcc ggcagcacac agcgtcaggc tatagaagtc gatgaagaag
    19561 gacaagtcag cggccaaacc acaccgagta aggacagcga tgcccgttgg atcaagaaaa
    19621 acggcctcta caaactcggt tacaaacaac atacccgtac cgatgcagaa ggctatatcg
    19681 agaaactgca cattaccccc gccaatgctc atgagtgcaa acacctgtcg ccgttgttag
    19741 aagggatagc tgaaggtacg accgtctatg ccgaca
//
LOCUS       DAWFHY010000036        19450 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.036, whole genome shotgun sequence.
ACCESSION   DAWFHY010000036 DAWFHY010000000
VERSION     DAWFHY010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19450
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.036"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(173..682)
                     /locus_tag="WGQ68_001096"
     CDS             complement(173..682)
                     /locus_tag="WGQ68_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003662333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEZ0714907.1"
                     /translation="MTMILSILSLFFIIRLLFLAVSIKHEKALIAKGAKQYGKTNSTL
                     LAAVHTLYYLACFVWVWLSDTAFNGISLIGTLTVMASFVILSLIIKQLGEIWTVKIYI
                     LPNHQINRSWLFKTFRHPNYFLNIIPELIGIALLCQAWYVLLIGLPIYLLVLFKRIRQ
                     EEQAMATLF"
     gene            complement(1014..1346)
                     /locus_tag="WGQ68_001097"
     CDS             complement(1014..1346)
                     /locus_tag="WGQ68_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspG"
                     /protein_id="HEZ0714908.1"
                     /translation="MQILSFQPDIAERMLEGTEGESVNENAQFVRTDNGYWIAWHEGV
                     AALLAPDTPPGIPCFWVEGAESLEELCVMVERGEFDEVEEFDGDDDEWLETAQGCGHH
                     GDACACGH"
     gene            complement(1391..1498)
                     /locus_tag="WGQ68_001098"
                     /pseudo
     CDS             complement(1391..1498)
                     /locus_tag="WGQ68_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            1637..2473
                     /gene="cysT"
                     /locus_tag="WGQ68_001099"
     CDS             1637..2473
                     /gene="cysT"
                     /locus_tag="WGQ68_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HEZ0714909.1"
                     /translation="MLALKTPGVLPGFKLSLGLTVLCLSLLVVLPFAMMAAKAAEIGW
                     SGFWNTITEPNVLAAVWLSLRMSFYAMLTNVVFGTLVAWVLVRYEFPGKGLANALVDL
                     PFALPTAVTGIALATLYAPNGWIGRFFEPLGIKIAFTPVGIWIALVVVSLPFIVRAVQ
                     PVLEELSGEYEEAAATLGANRFTTFRRVLLPEITPALLTGAGMMFARATGEYGSVIFI
                     AGNIPMVSEILPLIITGKLEQFDVQGASAVALFMLLVSFVILFALNVMLWALGRRSGA
                     KG"
     gene            2725..3585
                     /gene="cysW"
                     /locus_tag="WGQ68_001100"
     CDS             2725..3585
                     /gene="cysW"
                     /locus_tag="WGQ68_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysW"
                     /protein_id="HEZ0714910.1"
                     /translation="MKPYSANPNLTEPRRLRVLLIAAALGFLLLMLVVPLVAVFYEAL
                     KGGWDLYLKSLNDPEAWSAIKLTLITALIVVPVNAVLGVAMAWLLTRFDFRGKQLLTT
                     LLDLPFSVSPVVAGLMFVLLFGAHTALGGWLEAQGIQIIFAIPGIILATLFVTFPFVA
                     REIIPLMQAQGDSEEQAALILGASGWQMFWRVTLPNIKWALLYGIILTNARAMGEFGA
                     VSVVSGHIRGETNTVPLLVEIFYNEYNFTGAFALSGVLALLALATLAVQNIITKLQDK
                     KLAAAERNAA"
     gene            3582..4655
                     /locus_tag="WGQ68_001101"
     CDS             3582..4655
                     /locus_tag="WGQ68_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HEZ0714911.1"
                     /translation="MSITIQNLNKHFGAFHALKNINLNVPTGKLVSLLGPSGCGKTTL
                     LRIIAGLENADGGNILFDGQDVTAKHVRERKVGFVFQHYALFRHMNVFDNVAFGLTVL
                     PKSERPSKGQICTKVEELLKLVQLSHLAKSYPHQLSGGQRQRIALARALAVEPKLLLL
                     DEPFGALDAKVRKELRTWLRDIHHNLGVTSILVTHDQEEALEVSDEIVVMNHGKIEQT
                     GSAEAIYRKPENAFVTEFLGETDAFEGRIEKGFWHYNGFAWKLDAQYKWQEQTAIGYI
                     RPHEWQIAAEHETPMICAEIEKVHAVGALTHILVKHGKQDVHITLAGSDAARYPIAEG
                     KELKLIPKQVYVFSQNELIEYSI"
     gene            complement(4712..6238)
                     /gene="ilvA"
                     /locus_tag="WGQ68_001102"
     CDS             complement(4712..6238)
                     /gene="ilvA"
                     /locus_tag="WGQ68_001102"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HEZ0714912.1"
                     /translation="MNTPLPYSDYLIRILTASVYDVAVETPLEPARSLSVRLKNNILL
                     KREDLQPVFSFKIRGAYNKMSKLPKDALACGVIAASAGNHAQGVALSAQRLGCRAVIV
                     MPETTPKIKVDAVKSRGGEVVLRGVSYNDAYDYAMELAEKEGLTYIAPFDDHDVIAGQ
                     GTVGMEIVSQHPDPIRAVFVPIGGGGLAAGVAAFIKQVRPEIKVIGVQTNDSCCMKQS
                     VEAGEIVHLKDVGLFSDGTAVKVVGNETFRLCKELLDEIITVDTDAVCGAVKDIFDDT
                     RSITEPAGALALAGLKAYIAREGAENQTLIAVTSGANMNFHRLRHVSERSELGEGNEG
                     IFAVTIPEERGSFLKFVNILGNRNITEFNYRYGDDEKAHIFVGLQAAGPQDLAVIGSR
                     LDEAGLPNVDLTNNEIAKIHIRYMVGGRTDKVENERLVSFEFPERPGALARFLNHMQG
                     GWNITLFHYRNHGADYGRILVGIDVPPHDAAAFDGFLESLGYSYHEETQNAAYKLFLA
                     "
     gene            6386..7555
                     /locus_tag="WGQ68_001103"
     CDS             6386..7555
                     /locus_tag="WGQ68_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEZ0714913.1"
                     /translation="MTAHKILPALLSIILGVSHATAASPAPNRPTAHAAPTLQTPETL
                     TAAHIVIDLQSRQTLSAKNINTPVEPAALTQLMTAYLVFKNMKSGNIRSEENLKIPES
                     AWASEGSRMFVRPGDTVSTDKLLKGMIALSANDAALTLAGRLGNGSIENFVQQMNKEA
                     RRLGMKNTVFKNPTGLSREGQVSTAKDLAQLSEALMRDFPEYYPLFSIKSFKFKNIEQ
                     NNRNILLYRDNNVNGLKAGHTESGGYNLAVSYSGNGRHILVITLGSESAETRASDNSK
                     LLNWALQAFDTPKIYPKGKTVAQIQISGGSKKTVRAGFLKEAYITLPHKEAKMAEQIL
                     ETIQPIPAPVKKGQILGKIKIRQNGYTIAEKEIVALENVKKRSRWQRLWACLTGQ"
     gene            complement(7680..8252)
                     /locus_tag="WGQ68_001104"
     CDS             complement(7680..8252)
                     /locus_tag="WGQ68_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003755233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEZ0714914.1"
                     /translation="MTYEIQASVREAQGTGASRRLRREGQIPGILYGEGQEPVAIAVD
                     HKTVFYALEKESFHTALIKLSLNGETKDVIVRDFQMHPFRREVQHIDFQAVKADQLVR
                     IRVPLHIVNAENSQAVKLQGGRVSLLNTSVEVVALPANIPAFLDLDCAEVVAGDILHL
                     SDIKLPEGVESVSLKRNENLAVATVTGKKR"
     gene            complement(8319..9302)
                     /locus_tag="WGQ68_001105"
     CDS             complement(8319..9302)
                     /locus_tag="WGQ68_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEZ0714915.1"
                     /translation="MAAYDSLMVFTGNANPELAQRVVRHLDISLGNASVSKFSDGEVA
                     VELLENVRGRDVFILQPTCAPTNDNLMEILTMADALKRASAGRITTAIPYFGYARQDR
                     RPRSVRVPISAKLVANMLYSAGIDRVLTVDLHADQIQGFFDIPVDNIYATPILLNDIK
                     QQRIENLTVVSPDIGGVVRARAVAKSLNADLAIIDKRRPKANVAEVMNIIGDIQGRTC
                     LIVDDMIDTANTLCKAAVALKERGAERVLAYASHAVFSGEAVSRIASSEIDQVVVTDT
                     IPLSEAAKQCDRIRQVTIAGLLAETVRRISNEESVSYLFNEEVMTGSMLLP"
     gene            complement(9469..9544)
                     /locus_tag="WGQ68_001106"
     tRNA            complement(9469..9544)
                     /locus_tag="WGQ68_001106"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9509..9511),aa:Gln,seq:ttg)
     gene            complement(9553..9628)
                     /locus_tag="WGQ68_001107"
     tRNA            complement(9553..9628)
                     /locus_tag="WGQ68_001107"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9593..9595),aa:Gln,seq:ttg)
     gene            complement(9641..9716)
                     /locus_tag="WGQ68_001108"
     tRNA            complement(9641..9716)
                     /locus_tag="WGQ68_001108"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9681..9683),aa:Gln,seq:ttg)
     gene            complement(9724..10569)
                     /gene="ispE"
                     /locus_tag="WGQ68_001109"
     CDS             complement(9724..10569)
                     /gene="ispE"
                     /locus_tag="WGQ68_001109"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEZ0714916.1"
                     /translation="MNIADGRQAFPAPAKLNLDLRITGKREDGYHNIESIFCLIDLQD
                     TVYLKSRDDGKIILHNPVDGMPQEADLSYRAASLLQKYARNPAGVEIWLDKKIPTGAG
                     LGGGSSDAATVLLVLNRWWQCGLTQRQLIDSGAALGADVPFFIFGKNAFARGIGDRLD
                     EMDIPKQWYVIVKPPVHVSTAKIFTHEGLTRNSASSIMPTFQNLQPFRNDMQAVVFKE
                     YPEVWKAYSELSRYGFALMTGSGACVFTACQDRNSAYNIYRQVSGLYEAYLAEGLSKH
                     PWLSV"
     gene            complement(10579..11160)
                     /locus_tag="WGQ68_001110"
     CDS             complement(10579..11160)
                     /locus_tag="WGQ68_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein LolB"
                     /protein_id="HEZ0714917.1"
                     /translation="MKHTVSASVILLLTACAQLPQNNENLWQPSEHTRSFTAEGRLAV
                     KAEGKGSYANFDWTYQPPVETININTPLGSTLGQLCQDRDGALAVDGKGNVYQAESAE
                     ELSRQLVGFKLPIQYLHIWADGRRVAGAPYRILPDGILEQYGWTVGRTADSGGQVRTL
                     QLNNGNLNIRLVFTEIGMPSETETPERCAARTR"
     gene            complement(11300..13144)
                     /locus_tag="WGQ68_001111"
     CDS             complement(11300..13144)
                     /locus_tag="WGQ68_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714918.1"
                     /translation="MLPNRFKMLTVLTATLIAGQVSAAGGGAGDMKQPKEVGKVFRKQ
                     QRYSEEEIKNERARLAAVGERVNQIFTLLGGETALQKGQAGTALATYMLMLERTKSPE
                     VAERALEMAVSLNAFEQAEMIYQKWRQIEPIPGKAQKRAGWLRNVLRERGNQHLDGLE
                     EVLAQADEGQNRRVFLLLAQAAVQQDGLAQKASKAVRRAALKYEHLPEAAVADVVFSV
                     QGREKEKAIGALQRLAKLDTEILPPTLMTLRLTARKYPEILDGFFEQTDTQNLSAVWQ
                     EMEIMNLVSLHRLDDAYARLNVLLERNPNADLYIQAAILAANRKEGASVIDGYAEKAY
                     GRGTEEQRSRAALTAAMMYADRRDYAKVRQWLKKVSAPEYLFDKGVLAAAAAVELDGG
                     RAALRQIGRVRKLPEQQGRYFTADNLSKIQMLALSKLPDKREALRGLDKIIEKPPAGS
                     NTELQAEALVQRSVVYDRLGKRKKMISDLERAFRLAPDNAQIMNNLGYSLLTDSKRLD
                     EGFALLQTAYQINPDDTAVNDSIGWAYYLKGDAESALPYLRYSFENDPEPEVAAHLGE
                     VLWALGERDQAVDVWTQAAHLTGDKKIWRETLKRHGIALPQPSRKPRK"
     gene            complement(13309..14145)
                     /locus_tag="WGQ68_001112"
     CDS             complement(13309..14145)
                     /locus_tag="WGQ68_001112"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEZ0714919.1"
                     /translation="MQIIHTIRELRAWRKNAGKVAFVPTMGNLHEGHLALVREAKKRA
                     DSVVVSIFVNRLQFGQGEDFDKYPRTLQQDADKLEAEGVAVVFAPDEKELYPNVEQRY
                     NVEPPNLQNELCGKFRPGHFRGVATVVSKLFNIVAPDVACFGKKDYQQLAVIKGLTED
                     LNFDVEIVPVDTGRAEDGLALSSRNQYLSAAERDEAPRLYRELKAVAESLAQGSLDYA
                     GLEKRAVQSLTEYGWVVDYVEIRRVDTLEVARAGDKKLVVLAAARLGTTRLIDNLEIK
                     LP"
     gene            complement(14102..14278)
                     /locus_tag="WGQ68_001113"
     CDS             complement(14102..14278)
                     /locus_tag="WGQ68_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714920.1"
                     /translation="MQTEQGLLLMPSESRFRRHFVLPCAARIIGAFCRAGSPKDKDYR
                     YANHTYHSRTARVA"
     gene            complement(14268..15059)
                     /gene="panB"
                     /locus_tag="WGQ68_001114"
     CDS             complement(14268..15059)
                     /gene="panB"
                     /locus_tag="WGQ68_001114"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEZ0714921.1"
                     /translation="MITVNTLQKMKAAGEKIAMLTAYESSFAALMDDAGVEMLLVGDS
                     LGMAVQGRKSTLPVSLRDMCYHTECVARSAKNAMIVSDLPFGAYQQSKEQAFAAAAEL
                     MAAGAHMVKLEGGVWMAETTEFLQMRGIPVCAHIGLTPQSVFAFGGYKVQGRGGKAQA
                     LLNDAKAHDDAGAAVVLMECVPAELAKKVTETVSCPTIGIGAGADCDGQVLVMHDMLG
                     IFPGKTAKFVKNFMHGQSSIQAAVRAYVAEVKAKTFPAAEHIFAD"
     gene            complement(15201..15276)
                     /locus_tag="WGQ68_001115"
     tRNA            complement(15201..15276)
                     /locus_tag="WGQ68_001115"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15241..15243),aa:Thr,seq:tgt)
     gene            complement(15311..15386)
                     /locus_tag="WGQ68_001116"
     tRNA            complement(15311..15386)
                     /locus_tag="WGQ68_001116"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15351..15353),aa:Thr,seq:tgt)
     gene            15552..16343
                     /locus_tag="WGQ68_001117"
     CDS             15552..16343
                     /locus_tag="WGQ68_001117"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEZ0714922.1"
                     /translation="MARHPYRRLRPAKSSFPEVGISEEGNIRSLHLGSDTIQSSMNLD
                     HPSELVLSYSRAMMGWLLFTDALPQHITQIGLGGGSFARWIDTYLPDTRQTAVDINPQ
                     VIAIARNLFELPFEGEKFEIIEADGAEYIKVFRHNTDVILVDGFDGEQIIDALVEEPF
                     FRDCRNALSSDGIFVTNWWSGDKRYQRFIERLLSVFEGRVLELPAESHGNVAVMAFQS
                     SPKEQNIDKLKKRADKLSNAYGLDFHRMLAGLKASNPNNGKHFHL"
     gene            16398..17039
                     /locus_tag="WGQ68_001118"
     CDS             16398..17039
                     /locus_tag="WGQ68_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0714923.1"
                     /translation="MLKPETIHISGPAGILETIHIPSEQVPARGVAVINHPNPLQGGT
                     NTNKVIQTAAKALSKLGFHCYLPNLRGVGGSEGTHDYGRGETQDCLAVIDYARAQHPE
                     APEFALSGFSFGGYVATFAAQARIPDLLLLIGAAVCHYTGRPEPSAVPDVAKTLMIHG
                     AEDEVVEIGKALKWAEPQDLPVITIAGSTHFFHGKLIVLRDTILRFAPVCLNG"
     gene            complement(17117..18040)
                     /locus_tag="WGQ68_001119"
     CDS             complement(17117..18040)
                     /locus_tag="WGQ68_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0714924.1"
                     /translation="MKKRNNPLPLLNGVKPSYLVLPHEKQFYGLPLLHFLCIRFPFVG
                     ADDWRRRLNSGFVVGSDGAALDEHSLFEPGKTMFYYRETSSEDEPRIPFEEKILHIDE
                     HLIVVDKPHFLPVIPSGRFLRETLLTRLRLRPELQHLNVEDITPLHRLDKDTAGVMLL
                     SHNPATRGAYQTMFQNKTVWKTYEALAPTRTDLPYPLDVVSRLVRGEKFFTTQEAEGE
                     PNAHTTVELIENRGEFSLYRLTPHTGKKHQLRVHMMGLGMPLLNDALYPVPSEAGSED
                     YRKPLKLLAKKIAFADPLSGRERVFCSRFCL"
     gene            18204..19073
                     /locus_tag="WGQ68_001120"
     CDS             18204..19073
                     /locus_tag="WGQ68_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="meta-pathway of phenol degradation family
                     protein"
                     /protein_id="HEZ0714925.1"
                     /translation="MKRIFLPALPAILPLSAYADLPLTIEDIMTDKGKWKLKTSLTYL
                     NSENNRAELAAPVYIQTGATSFIPIPTEIQENGSNTDMLVGTLGLRYGLTGNTDIYGS
                     GSYLWHEERKLDGNGKTRNKRMSDVSLGISHTFLKDDKNPALISFLESTVYEKSRNKA
                     SSGKSWLIGATTYKAIDPIVLSLTAAYRINGSKTLSDGIRYKSGNYLLLNPNISFAAN
                     DRISLTGGIQWLGTQPDRIDGKKETARNTSTYAHFGTGFGFTKTTALNASARFNVSGQ
                     SSSELKFGVQHTF"
     gene            19116..>19450
                     /locus_tag="WGQ68_001121"
     CDS             19116..>19450
                     /locus_tag="WGQ68_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714926.1"
                     /translation="MKKQITSAVMMLSMIAPAMANGLDNQTFENQVFHTQADAPMQLA
                     ELSQKEMKETEGEWIPNAVGGIMGGVGGHFSYMASAIASGSYNREAHWTAIGGGALIG
                     AMNPINGGRA"
BASE COUNT         4843 a         5503 c         4530 g         4574 t
ORIGIN      
        1 gcgggaatga cggaaagtgg cgggaatgac ggttcgggca ttccttaaat tacccgtgta
       61 tcgctgtaaa tcttagagat ggcggaatat agcggattaa caaaaaccag tacggcaagg
      121 cgaggcaacg ccgtactggt ttttgttaat ccgctataat tgatgaaacg ggttaaaaaa
      181 gtgttgccat cgcctgttct tcttgtcgga tacgcttaaa taagaccagc aaataaatgg
      241 gcaggccaat caataaaaca taccacgctt gacataataa ggcgatgcca atcagttcgg
      301 gtatgatgtt taaaaaataa ttggggtggc ggaatgtttt aaacaaccac gaacgattaa
      361 tttgatgatt tggtaaaata tagattttaa ccgtccaaat ctcccccaac tgcttaataa
      421 tcaatgacaa tatcacaaac gaagccatca ccgtcagcgt accaatcaag gatatgccat
      481 taaaagcagt gtcagaaagc catacccaaa caaaacacgc caaataataa agcgtatgaa
      541 ctgccgcaag cagcgtggaa ttggtttttc cgtattgttt tgcccctttg gcaatcaagg
      601 ctttttcatg tttaatagag acggctaaaa ataacagtct gatgataaaa aacaggctta
      661 aaatgcttaa aatcattgtc attgatgttt ttccattgaa attgaaataa atataaatcg
      721 gattaatggt atttttaatt aatgatgttt cagaccatca tgctctataa acaattccca
      781 ttaagtccgc gccgcaacct gctataataa gtctgcaatc ggcgcaaatc aatgctttgc
      841 gtttattgcc atcccaaaat aattgatgct gccttaatta taataccaag ataagttttt
      901 ttattcaata aaatacaaag ggaagcgttc agcccattgc aaacagatgc aatccaccga
      961 ttatttaaaa aacggcaaag ccttgccccc cttgcggcaa gcctgcaatg cctttaatgt
     1021 ccgcaggcgc aagcgtcgcc gtggtgcccg caaccctgtg ccgtttcgag ccattcgtcg
     1081 tcatcgccgt caaactcttc cacttcgtca aactcgccgc gttccaccat gacgcacaac
     1141 tcttcaaggc tttccgcgcc ttccacccaa aaacagggaa tgcccggcgg cgtatcgggc
     1201 gcaagcagtg ccgctacgcc ttcatgccac gcaatccaat agccgttgtc cgtacggacg
     1261 aattgtgcgt tttcgttgac cgactcgcct tccgtacctt ccagcatacg ttccgcaatg
     1321 tccggctgaa aagataaaat ctgcataagt gttcctttat atgatggttt tccgtcaaaa
     1381 caaggcgtta tagtggatta aatttaaacc agtacggcgt tgcctcgcct tgccgtacta
     1441 tttgtactgt ctgcggcttc gtcgccttgt cctgatttaa atttaatcca ctatatttta
     1501 aaacatcgcg cccgcttgag aaactgccaa ccgctttata acaaattcgt ctttgcacca
     1561 aactttccat tctttccgtt tttcggacgg catcgttaaa gtagtccttc cttttcctta
     1621 ttttcagcat tgttttatgt tagcgctcaa aacgcccggc gtactgcccg gcttcaaact
     1681 cagccttggt ctgaccgtat tgtgcctgtc gctgcttgtg gtcttgccgt ttgcgatgat
     1741 ggcggcgaag gcggcggaaa tcggctggag cggattttgg aacacgatta ccgaacccaa
     1801 tgtcttggcg gccgtctggc tgagtttgcg gatgtcgttt tatgcgatgc tgaccaatgt
     1861 cgtgttcggc acgctggtgg cgtgggtatt ggtgcgttat gaattcccgg gcaagggtct
     1921 ggcgaacgcg ctggtcgatt tgccgtttgc gctgccgacg gcggttacgg gtatcgcgtt
     1981 ggcgaccctg tatgcgccca acggttggat aggccgtttt ttcgagcctt tgggcatcaa
     2041 aatcgcgttt acgcccgtcg gcatttggat tgcgctggtc gtcgtcagcc tgccctttat
     2101 cgtccgcgcc gtgcagccgg tgttggagga attatcgggc gaatatgagg aagcggcggc
     2161 gactttgggc gcaaaccgct ttacgacgtt ccgccgcgtg ctgctgccgg aaatcacgcc
     2221 ggcactcttg accggcgcgg gaatgatgtt cgcgcgggca acgggggaat acggctcggt
     2281 gatttttatc gcgggcaata ttccgatggt ttctgaaatc ctgccgctga ttattacggg
     2341 caagctggaa cagttcgacg tacagggcgc gtcggcggtg gcgttgttta tgctgctggt
     2401 ttcgtttgtc attctgtttg cgctgaacgt gatgctgtgg gcgttgggca ggcgttcggg
     2461 cgcgaagggt tgaggtcgtc tgaaatacct gttaccgtca ttcccgcgca ggcgggaatc
     2521 cattggtgaa tttcggctgc cttatttatt ttctgttttc ctgttgccct gtggtggatt
     2581 cccgcctgcg cgggaatgac ggtagctaga cgtttttatt cccttaatca ataaaaggtt
     2641 gtctgaaaac gaatccgctc ccaaaaacac agtttttcag acgacctcca aacattttaa
     2701 aaccaaccag agaacaccac cgccatgaaa ccctattccg ccaatcccaa cctgaccgaa
     2761 ccgcgccggc tgcgcgtgtt gctgattgcc gccgcgctgg gctttctgct gctgatgctg
     2821 gtcgtgccgc tcgtcgccgt gttttacgaa gccttaaaag gcggttggga tttgtacctg
     2881 aaatccttaa acgatcccga agcgtggtct gccatcaaat tgacgctgat taccgcgctg
     2941 attgtcgttc ccgtcaatgc cgtcttgggc gtggcgatgg cgtggctgct gacccgtttt
     3001 gacttccgag gcaagcagtt gctgactacc ctgctcgatt tgccgttttc cgtatcgccc
     3061 gtggtggccg gtttaatgtt cgtcttattg ttcggcgcgc acacggcatt gggtggctgg
     3121 ctcgaagcgc aaggcataca gattatcttc gccatccccg gcattattct ggcgacgctg
     3181 ttcgttacct tcccctttgt cgcgcgcgaa atcatcccgc tgatgcaggc tcagggcgac
     3241 agcgaagaac aggcggcatt gatactcggc gcaagcggct ggcagatgtt ttggcgcgtt
     3301 accctgccca acatcaaatg ggcgttactc tacggcatca tcctcaccaa cgcccgcgcg
     3361 atgggcgagt tcggcgcggt cagcgtggta tcgggacaca tacgcggcga aaccaacacc
     3421 gtcccgcttt tggtcgaaat cttctacaac gaatacaact tcaccggcgc attcgccctc
     3481 tccggcgtat tggcactttt agcactggcg acgctggcgg tgcagaacat catcaccaaa
     3541 ttacaagaca aaaaactcgc cgccgccgaa aggaacgcag catgagcatt actattcaaa
     3601 atctaaataa acacttcgga gcgttccacg cgctgaaaaa catcaacctc aacgtcccca
     3661 ccggcaaact cgtttcccta ctcggccctt caggctgcgg caaaactaca cttttacgca
     3721 ttatcgccgg actggaaaac gccgacggcg gcaatattct ctttgacgga caagacgtaa
     3781 ccgccaaaca tgtgcgcgag cgcaaagtcg gcttcgtgtt ccaacactac gctcttttcc
     3841 gccatatgaa cgtgtttgac aacgtcgctt tcggtttgac cgtattgccc aagtccgaac
     3901 gcccgtccaa aggacaaatc tgcactaaag tcgaagaatt gctgaaactc gtacaactct
     3961 cgcatttggc aaaatcctat ccgcaccaac tctccggcgg gcaacgccag cgcatcgccc
     4021 tcgcccgtgc gcttgcggtc gaaccgaaac tcctgctttt ggacgaaccc ttcggcgcgt
     4081 tggatgccaa agtacgcaaa gaattacgca cctggctgcg cgacatccat cacaacctgg
     4141 gtgtaaccag cattttggtt acacacgacc aagaagaagc cctcgaagtt tccgacgaaa
     4201 tcgtcgtgat gaaccacggc aaaatcgaac aaaccggcag cgccgaagcc atttaccgca
     4261 aacccgaaaa tgccttcgtt accgagttcc tcggcgaaac cgacgcattt gaaggacgca
     4321 tcgaaaaagg cttctggcat tataacggct tcgcgtggaa attggacgca caatacaaat
     4381 ggcaggagca aaccgccatc ggctacatcc gtccgcacga atggcagatc gccgccgaac
     4441 acgaaacacc gatgatttgt gccgaaatcg aaaaagtcca cgccgtcggg gcattgacgc
     4501 atattttggt gaaacacggc aaacaagacg tgcacatcac gctggctggc agcgatgccg
     4561 cgcgttaccc aatcgccgaa ggcaaagaat tgaagctgat tccaaaacag gtttatgtct
     4621 tctctcaaaa cgaactgatt gaatattcga tttaaccatg aaagcgcaat gccgtctgaa
     4681 aggctttcag acggcattgt gctttcaagc gtcaggcaag aaacagcttg tacgcggcat
     4741 tttgcgtttc ctcatgatag ctgtatccca gactttccaa gaaaccgtca aatgcggcgg
     4801 catcgtgcgg cggcacatcg ataccgacca aaatccgccc gtaatccgca ccgtggttgc
     4861 ggtaatggaa aagcgtaata ttccaccctc cctgcatatg gttcaaaaag cgtgccaatg
     4921 cccccggacg ctccggaaac tcaaaactga ccagacgctc gttttctact ttgtccgtcc
     4981 gccctccgac catatagcgg atatggattt tggcaatctc attgttggtc aaatcgacat
     5041 tgggcaatcc cgcctcatcc aaccggctgc cgataaccgc caaatcctgc gggcctgccg
     5101 cttgaagtcc gacaaagata tgcgcttttt catcgtcccc gtagcggtag ttgaactcgg
     5161 taatattcct atttcccaat atattgacaa acttaaggaa gctgccgcgc tcttcgggaa
     5221 tggtaacggc aaaaatacct tcgttgccct cgcccaattc gctccgttcc gaaacgtggc
     5281 gcaaacggtg aaaattcata ttcgcgccgc tggtaacggc aatcagggtt tggttttccg
     5341 cgccttctcg ggcaatatag gctttcagac ccgccaacgc caacgctccg gccggctcgg
     5401 taatgctgcg cgtgtcatcg aaaatatcct tgaccgcgcc gcaaaccgca tcggtatcga
     5461 ctgtaatgat ttcatccaaa agttctttgc agaggcggaa ggtttcgttt ccgacgactt
     5521 tgaccgcagt gccgtctgaa aacagcccga catctttcaa atggacgatt tcacccgctt
     5581 cgaccgactg cttcatacag caggaatcgt tggtctgaac gccgataact ttgatttcgg
     5641 ggcggacctg cttgataaat gccgccacgc ccgccgccaa accgccaccg cctatcggta
     5701 cgaatacggc gcggattgga tcgggatgct ggctgacaat ttccatcccc accgtcccct
     5761 gtcccgcaat cacatcatga tcatcaaacg gcgcgatata ggttaaccct tctttttccg
     5821 ccaactccat cgcataatcg taggcatcgt tgtatgaaac gccccgcaaa accacctcgc
     5881 cgccacggct cttaaccgca tccactttga ttttcggcgt agtctccggc ataacgataa
     5941 cggcacggca gcccaaacgc tgtgcggaca atgccacgcc ttgagcatga ttgcccgcgc
     6001 ttgccgcaat cacgccgcaa gcgagcgcat ctttcggcaa cttggacatt ttgttgtacg
     6061 cgccgcgtat tttgaacgaa aaaaccggct gcaaatcttc gcgtttcaaa aggatgttgt
     6121 ttttcaaacg tacagaaagg ctgcgtgccg gttccaaagg cgtttcgacc gcgacatcat
     6181 agacagatgc cgtcaggatg cggatgaggt aatcggaata aggaaggggc gtgttcataa
     6241 ttcaatatgg gataatcggt ttattaaaat cgcaaaaccc aaaaccatac gcccaagacg
     6301 gcgcgaaatc aagaaaaatc cgcccgatca gacaccctaa gcgtataatc ggcaggctga
     6361 aacacgcaca caattagaat atttcatgac agcacataaa atcctgcccg ccctgctttc
     6421 catcatctta ggcgtttctc atgcaacggc tgcatcgccc gcgcccaaca gaccgacggc
     6481 acacgccgcc cccacgctcc aaacacccga aaccctcaca gcggcacaca tcgttatcga
     6541 ccttcaaagc agacagactt tatccgccaa aaacatcaat acccctgtcg aaccggcggc
     6601 actaacccaa ctgatgaccg catatctggt tttcaaaaac atgaaatcgg gaaatatccg
     6661 atctgaagaa aacttaaaaa tacccgaatc cgcatgggct tcagaaggaa gcagaatgtt
     6721 tgtacgtccc ggcgatacgg tcagcaccga caaactctta aaaggcatga ttgcactatc
     6781 cgcaaacgat gccgccctaa cccttgccgg ccggctgggc aacggctcga ttgaaaattt
     6841 tgtgcaacaa atgaacaaag aagcccgacg cttgggcatg aagaacactg tattcaaaaa
     6901 tccgacaggc ttgagtagag aaggacaggt ttccaccgcc aaagacctcg cccagctgtc
     6961 tgaagcattg atgcgcgact ttccggaata ttacccgctg ttttccatca aatctttcaa
     7021 attcaaaaat atagagcaaa acaaccgcaa tatcctttta tatagggaca acaatgtaaa
     7081 cggtctgaaa gccggacaca cagaaagcgg cggctacaac cttgccgtgt catactccgg
     7141 caacggcagg cacatccttg tcatcacatt gggttcggaa tcggcggaaa cacgcgcatc
     7201 ggacaacagc aagctactga actgggcatt gcaggctttc gatacgccca aaatatatcc
     7261 gaaaggcaaa accgttgccc aaatccaaat ttccggaggc agcaaaaaaa ccgtccgcgc
     7321 aggcttcctc aaagaagcct acatcactct gccacataag gaagcgaaaa tggcagaaca
     7381 aattctagaa accatacagc cgattcccgc cccagtaaaa aaagggcaaa ttttaggaaa
     7441 aatcaaaatc agacaaaacg gatacaccat tgccgaaaaa gaaatcgtcg cactggaaaa
     7501 tgtaaaaaaa agaagccggt ggcaaaggct ttgggcgtgt ctgacagggc agtaatctgc
     7561 cgtttcaaat atcccgtttt tccaacaaat aaagaaatgc cgtctgaaac acggttcaga
     7621 cggcataaaa caacagggcg gtacgtattg catacgcgcc gccctgctgc tgaaatcaat
     7681 tagcgtttct taccggtaac ggtagcaaca gccagatttt cgttacgttt cagggaaacg
     7741 ctttctacac cttcaggcag tttgatgtct gacaagtgca gaatgtcgcc ggcaaccact
     7801 tcggcacaat ccaaatccaa gaaagcaggg atgttggcag gcaaagcaac tacttcaaca
     7861 gaagtgttta acagagatac gcggccgcct tgcagtttga ccgcttggga attttcagcg
     7921 ttaacgatgt gcaggggaac acggatgcgt acaagttgat cggctttcac agcttggaag
     7981 tcgatgtgtt gaacttcgcg gcggaacggg tgcatttgga aatcacggac gataacgtct
     8041 ttggtttcac cgttcagaga caacttaatc aacgcagtat ggaaagattc tttttccaat
     8101 gcgtagaata cggttttgtg atccacagcg attgcaacag gctcttgacc ttcaccgtac
     8161 agaatgccgg ggatttggcc ttcgcgacgc aggcggcggc tcgcaccagt gccttgtgct
     8221 tcacgaacag aagcttgaat ttcataagtc atgttaaata ctccaagtta ggtaaaatcg
     8281 ccgtcatcgg ccgcgaccag cttaaaacgg cttcgggctt atggcagcaa catgctgcct
     8341 gtcatcactt cttcattaaa aagatatgag acggattctt cgttgctgat gcggcggacg
     8401 gtttcggcca acagaccggc aatcgttacc tgacggatac ggtcgcattg tttggccgct
     8461 tcagacaaag gaatggtatc ggttacgacc acctggtcga tttcggacga ggcaatacgg
     8521 ctgaccgcct ctccggagaa tactgcatgg ctggcatatg ccaagacacg ttccgcaccg
     8581 cgctctttca gggcgacggc agctttacac aaggtattcg cggtgtcaat catatcatcc
     8641 acaatcaagc aagtacgacc ttgaatatcg ccgataatgt tcatgacttc cgccacattg
     8701 gctttcgggc ggcgtttgtc gataatcgcc aaatcggcat ttaatgattt tgccacggcg
     8761 cgggcgcgga cgacaccgcc gatgtccggg ctgacgacgg tcagattttc aatccgctgt
     8821 tgtttgatgt cgttcaacag aatcggggtg gcataaatat tgtccaccgg aatatcgaag
     8881 aaaccttgaa tctggtcggc atgcaaatcg acagtcaaaa cacggtcgat ccctgccgaa
     8941 tacagcatat ttgccaccag tttggcagaa atcggaacgc ggacggaacg cggacggcgg
     9001 tcttggcgcg catagccgaa atacggaatg gctgtggtaa tacgacctgc cgaagcacgc
     9061 ttcagtgcat ccgccatcgt caggatttcc atcaggttgt cattggtcgg cgcacaggtc
     9121 ggctgaagga tgaaaacatc gcgcccgcgt acgttttcca acagttcgac ggcaacttcg
     9181 ccgtctgaaa acttggatac ggaagcattg cccaaagaaa tgtccaaatg cctgacaaca
     9241 cgttgtgcca attcgggatt ggcattgcct gtaaatacca tcaaactgtc gtacgcagcc
     9301 atattctcac ctgattttat gtttaacttc cgctcagaaa acacaatgct tccctctgct
     9361 tgtaccaagc ccgacattat acctgcaaca tccgacacaa acaaaggcat ttcaatattt
     9421 ttatttctat gaaataaaag cgtgtaagca ggcttacacg cttttatctg gctggggagg
     9481 aaggattcga accttcgcat gctggaatca aaatccagtg tcttaaccgc ttgacgactc
     9541 cccaaaaagg gctggctggg gaggaaggat tcgaaccttc gcatgctgga atcaaaatcc
     9601 agtgtcttaa ccgcttgacg actccccaac tcgtttgact tggctgggga ggaaggattc
     9661 gaaccttcgc atgctggaat caaaatccag tgtcttaacc gcttgacgac tccccaacaa
     9721 tgtttatacg gacaaccaag gatgttttga aagaccctct gccaaatatg cctcgtacaa
     9781 acctgaaact tgtcggtata tattgtatgc gctattccta tcttgacacg ccgtgaatac
     9841 acacgcacca gaacctgtca ttaaggcaaa tccatatcgg gacaactcgg aataggcttt
     9901 ccaaacttca gggtattctt taaatacaac tgcctgcata tcatttctaa acggttgcag
     9961 gttttggaaa gtcggcatta tgcttgaggc ggaatttcgt gtcaagcctt cgtgtgtgaa
    10021 aatttttgca gtggaaacgt ggacgggcgg tttgacgatg acataccact gtttcggaat
    10081 atccatttcg tccagcctgt cgcctatacc ccgcgcaaac gcatttttgc cgaaaataaa
    10141 aaacggtacg tccgccccca gagccgcgcc cgaatcaatg agctgccgct gcgtcagacc
    10201 gcactgccac caacggttca acaccagcaa aacggttgcc gcatccgagc ttccgccgcc
    10261 caaacccgcc cctgtcggga tttttttgtc cagccatatt tccacgccgg cggggttgcg
    10321 cgcatatttt tgcagcaacg atgcggcacg gtagcttaaa tccgcttcct gcggcattcc
    10381 atcaaccgga ttgtgcagga tgattttgcc gtcatccctc gatttcaaat atacggtatc
    10441 ctgcaaatct atcaggcaga atatgctttc gatattgtga taaccgtctt cccgctttcc
    10501 agtaatcctc aaatcgagat tcagttttgc aggtgcggga aacgcctgcc gtccgtccgc
    10561 aatattcatc tgtccgcctt atctcgtgcg cgccgcacag cgttccgggg tttcagtttc
    10621 agacggcata ccgatttcgg tgaaaaccag cctgatgttc aaatttccgt tattcagttg
    10681 caacgttcgg acttgccccc cactgtcggc ggttctgccg acagtccaac cgtattgttc
    10741 caatatgccg tccggcagga tgcggtaagg cgcgcccgcc acacgcctgc catctgccca
    10801 gatatgcaga tattggattg gtagtttgaa accgaccagc tgcctgctta attcttccgc
    10861 actttccgcc tgatagacat ttcctttgcc gtccactgcc aatgcgccgt ccctgtcttg
    10921 acacaactgc ccgagcgtac tgcccaaagg ggtattgata ttgatggttt ccacgggcgg
    10981 ttggtatgtc caatcgaaat ttgcatacga acctttccct tccgctttca ctgccaaccg
    11041 cccttccgcc gtgaaactgc gggtgtgttc ggacggctgc cacaggtttt cgttattttg
    11101 aggtaattgc gcgcaagcgg tcaaaagcag gatgaccgat gcggatacag tgtgtttcat
    11161 cagaatttcc ttaacggatg ccatcctgcc ggccgatggg ttcggcgtgc cgtgcaaaac
    11221 gcgtgcgaac tgcgtgcggt ttgcccgaat gccgcagctt cccggcaaac cttatgccgt
    11281 ctgaaaggat ggacctgcat tatttccgag gttttcggga aggttggggc aatgcgatgc
    11341 cgtgacgttt gagcgtttcc cgccatattt tcttgtctcc cgtaaggtgt gccgcctgcg
    11401 tccatacgtc aaccgcctga tcgcgttcgc ccaatgccca caacacttcg cccaaatggg
    11461 cggcaacttc gggctcgggg tcgttttcaa acgaataccg cagatacggc agcgcgcttt
    11521 ccgcgtcgcc tttcaggtaa tacgcccagc ctatgctgtc gttgacagcg gtatcgtccg
    11581 ggttgatttg gtatgccgtc tgaagcaggg cgaaaccttc gtccaaacgt ttggaatcgg
    11641 tcagcaggct gtagcccaga ttattcataa tctgagcgtt atcgggtgca agcctgaacg
    11701 ccctttcaag atctgaaatc atttttttcc gcttgccaag ccgatcgtaa acaactgacc
    11761 gctgtaccaa tgcctctgcc tgtaactctg tattactgcc ggcaggcggt ttttcgataa
    11821 tcttgtccaa ccccctcaaa gcctcccgtt tatcgggcag cttcgacagg gcgagcatct
    11881 gtattttgga caaattgtct gccgtaaaat accgcccctg ctgttcggga agtttccgca
    11941 ccctgccgat ctgccgcaaa gccgccctgc cgccgtccaa ctcgacagcc gccgcagccg
    12001 ccagcacacc tttgtcgaac aggtattccg gcgcggatac ttttttcagc cactgcctga
    12061 ctttggcgta atccctgcgg tcggcataca tcatcgccgc cgttagcgcc gccctgctcc
    12121 gctgttcctc cgtccccctg ccgtatgcct tttcggcgta gccgtcgata acggaagcac
    12181 cttcttttcg gtttgccgcc aatatcgctg cctgaatata caggtctgca ttcggattgc
    12241 gttccaacag cacgttcaaa cgcgcatagg catcatccag cctgtgcagg gaaaccagat
    12301 tcataatttc catttcctgc cagacggccg aaaggttttg ggtgtctgtc tgctcgaaaa
    12361 agccgtcgag tatttcggga tatttgcgtg cagtcagacg caacgtcatt aaagtggggg
    12421 gcaatatttc cgtatcgagc ttcgccaaac gctgcaaagc tccgattgcc ttttcctttt
    12481 cgcgtccctg tacgctgaac accacatcgg caaccgccgc ttcgggcaga tgttcatatt
    12541 tcaacgccgc gcggcgaacc gctttcgatg ctttttgcgc caacccgtcc tgttgcacgg
    12601 cggcttgtgc caacaataaa aacaccctgc ggttctgtcc ttcgtccgcc tgagccagca
    12661 cttcttccag tccgtccaga tgctgatttc ctctttccct cagcacgttc cgcagccacc
    12721 ccgcccgttt ttgcgcctta cccggtatag gctcaatctg ccgccatttc tgataaatca
    12781 tttccgcctg ttcaaacgcg ttcagcgaca cggccatttc caaggcgcgt tcggcgactt
    12841 cgggggattt tgtgcgttcc aacatcagca tataggttgc cagagccgtt cccgcctgcc
    12901 ccttttgcaa ggcggtttcc cctcccagca acgtaaatat ctgattaacc cgctcgccca
    12961 ctgccgcaag ccgtgcgcgt tcgtttttga tttcttcctc gctgtaacgc tgctgctttc
    13021 tgaaaacctt tccgacttcc ttcggctgtt tcatatcccc cgcaccgcct ccggcggcag
    13081 atacctgtcc ggcaatcaag gttgccgtca acacagttaa cattttgaaa cggttaggta
    13141 acataatcat cctttatccg acagccgtcc gaaccttcag acggcatata atcaggcgta
    13201 actttatcac aagcagaatc gtttgtagat ttgcgaaacc ccgttcaaaa aaataaatgc
    13261 cgtctgaaac gcaaatccgc ttcagacggc attcccgacg cttgcggttt aagggagttt
    13321 tatttccaaa ttgtcaatca ggcgcgtcgt ccccagacgg gcggcggcca agaccaccag
    13381 tttcttatct cccgcccgcg ccacttcgag cgtatcgacg cggcggattt cgacataatc
    13441 gaccacccag ccgtattctg tcagggattg gacggcacgt ttttccaaac ctgcataatc
    13501 caaactgccc tgcgccaagg attcggcaac agcctttaat tcgcggtaca ggcgcggtgc
    13561 ttcgtcgcgt tccgccgcac tcaaatactg gttgcggctg gagagtgcca aaccgtcttc
    13621 cgcgcgcccc gtatcgacag gcactatttc aacatcaaaa ttcaaatcct cggtcaaacc
    13681 tttaatcacg gcaagctgct gatagtcttt tttgccgaaa caggcaacat ccggcgcgac
    13741 aatattgaac agcttggaca ctaccgttgc caccccgcgg aaatgtcccg gacgaaactt
    13801 gccgcacaac tcattttgca gattgggcgg ttcgacgttg taacgctgtt ccacgttcgg
    13861 atagagttct ttctcatcgg gcgcgaaaac aacggcaaca ccctcggctt ccagcttgtc
    13921 cgcatcctgt tgcaaagtac gcggatattt gtcgaaatcc tcgccctgac cgaattgcag
    13981 gcggttgacg aaaatgctga ccacgacact gtccgcgcgt tttttcgcct cacgcacaag
    14041 cgcaagatgc ccttcatgca gattgcccat ggtcggcaca aatgccacct ttcccgcatt
    14101 tttacgccac gcgcgcagtt ctcgaatggt atgtatgatt tgcataacgg taatccttat
    14161 ccttcgggct tcctgcccga caaaacgcgc cgattatacg cgccgcgcac ggcaaaacaa
    14221 aatgccgtct gaaacggctt tcagacggca tcagcagcaa accttgttca gtctgcaaaa
    14281 atatgttccg cagcagggaa ggttttggct ttgacttcgg caacatacgc ccgaaccgcc
    14341 gcctgaatgc tgctttgccc gtgcataaag tttttgacga atttcgccgt cttacccggg
    14401 aaaatgccca gcatatcgtg catcaccaaa acctgaccgt cgcaatccgc gcccgcaccg
    14461 atgccgatgg tcggacagga aacagtttca gttacctttt ttgccagttc cgccggtacg
    14521 cactccatca gcacgaccgc cgcccccgca tcgtcatgcg ccttggcatc gttaagcaac
    14581 gcctgcgcct tgccgccgcg cccctgaacc ttatatccgc caaaggcaaa cacggattgc
    14641 ggcgtcagac cgatgtgggc acagaccgga ataccgcgca tttgcagaaa ttcagtcgtt
    14701 tccgccatcc acacgccgcc ttcgagtttg accatatgcg cgccggccgc catcagttcg
    14761 gcggcggcgg caaacgcctg ctccttactc tgctgatatg caccaaacgg caaatcgctg
    14821 acaatcatcg cattttttgc actgcgtgct acacattcgg tgtgatagca catatcgcgc
    14881 aggctgacgg gcagcgtcga tttccgcccc tgaaccgcca tccccaaaga atcgccgacc
    14941 agcagcattt ccacgccggc atcgtccatc agtgcggcaa aactggattc gtaagcggtc
    15001 agcatagcga ttttttcgcc cgccgccttc attttttgca gtgtgttcac agtaatcatc
    15061 agatattgtt accccgccct tttcagacgg ccttttcagt ttgaaacggg ctattatagt
    15121 gaaatgcccg cagccctgcc accgaccgtg ccgcaaatgc aaaacagccc gactgtttta
    15181 cacaatcgga ctgtcaaatc tggtgccggc accaagagtc gaactcggga ccccctgatt
    15241 acaagtcagg tgctctacca actgagctat accggcttac tgaaatgttt tgcaacacca
    15301 tcagcagaaa tggtgccggc accaagagtc gaactcggga ccccctgatt acaagtcagg
    15361 tgctctacca actgagctat accggcaaag aaagcgaatt atgcaggcaa tgccgcacct
    15421 tggcaaggaa tttatccgaa agccccggca aatccgaata agtaatttat ttgaaaagaa
    15481 attaatttga agcaggacaa ccatcggcat tctgataaaa tatctgcctt tcaaaccgac
    15541 cgttcacacc gatggcaaga catccctacc gccgcctgcg tcctgcaaaa tccagctttc
    15601 ccgaagtcgg catttccgaa gaaggcaata tccgttcgct tcacttgggc agcgacacca
    15661 tccaaagctc aatgaacctc gaccacccgt ccgagctggt actctcttac agccgtgcca
    15721 tgatgggatg gctgctgttt accgacgctc ttccgcagca tatcacccaa atcggcttgg
    15781 gcggcggttc gtttgcacgc tggatagaca cctacctgcc cgacacgcgc caaaccgccg
    15841 tggacatcaa cccgcaggtt atcgccattg cccgcaacct gtttgagttg cctttcgagg
    15901 gcgagaaatt tgaaattatt gaagcagacg gtgcggaata tatcaaagtc ttccgccaca
    15961 acaccgatgt gattttggtg gacgggttcg acggcgaaca aatcatcgat gcgctggttg
    16021 aagaaccgtt tttccgagac tgccgaaacg cactctcttc agacggcata ttcgtaacca
    16081 actggtggag cggcgacaaa cgctaccaac gcttcatcga acggttgttg agcgtttttg
    16141 aagggcgcgt cttggaactt cctgccgaaa gccacggcaa tgtcgcggta atggccttcc
    16201 aaagcagccc caaagagcaa aacatagaca aactcaaaaa acgtgccgac aaactgagca
    16261 acgcatacgg attggacttc caccgtatgc ttgccggcct gaaagcgtcc aaccccaaca
    16321 acggcaagca tttccacctt taagcaacta tcttcacggc tgcattggaa catgcggaaa
    16381 atcaagagga aatcattatg ctgaaacccg aaaccataca catatccggt cctgccggca
    16441 ttttagaaac catccatatc ccgtccgaac aagtaccggc acgcggtgtt gccgtcatca
    16501 atcatcccaa ccccctccag ggcggaacaa acaccaacaa agttatccaa actgccgcca
    16561 aagccttaag caaactcggc ttccactgct acctgcccaa ccttcgcggc gtaggcggca
    16621 gcgaaggcac acacgattac ggacgcggcg aaacgcaaga ctgcctcgcc gtcatcgact
    16681 atgcccgtgc ccaacatccc gaagcccccg aatttgcctt atccggcttc tccttcggcg
    16741 gttatgtcgc cacatttgcc gcacaagcgc gcatacccga tttattgctg ctcatcggcg
    16801 cggcagtctg ccactatacc ggccgtccgg aaccgtcagc cgttcccgac gttgcaaaaa
    16861 cgctgatgat acacggcgcg gaagatgaag tcgtcgaaat cgggaaagca ttgaaatggg
    16921 cagaaccgca agatttgccc gtcattacca tcgccggttc cacgcatttc tttcacggca
    16981 aactcatcgt cctgcgcgac accatcctcc gcttcgcgcc cgtatgcctg aacggataaa
    17041 acccctttca gacggcatcg ataatatcaa acccgtatcg gcggcatctg ccgatacggg
    17101 tttgcattcc ctctcattat aggcaaaacc tactgcaaaa caccctttcc cgacccgaca
    17161 aaggatctgc aaacgcaatc ttttttgcca ataacttcaa aggtttccga taatcctcgc
    17221 tgcccgcttc agacggcacg ggatagagcg cgtcattcaa cagcggcata cccaaaccca
    17281 tcatatgcac gcgcaactgg tgtttcttgc cggtatgcgg cgtaaggcgg taaaggctga
    17341 attcccccct gttttcaatc agttcgaccg tcgtatgtgc gtttggttcg ccttccgcct
    17401 cttgcgtcgt aaaaaatttc tcacccctca ccaaacgcga aaccacatcg agcggatacg
    17461 gcaaatccgt ccttgtcggc gcaagtgcct catacgtttt ccataccgtt ttgttttgaa
    17521 acatcgtctg ataagccccg cgcgtggcag gattatgcga cagcagcatc acgcctgccg
    17581 tatccttgtc caagcggtgc agcggcgtaa tgtcctcaac attcaaatgc tgcaattcag
    17641 gacgcaggcg cagacgtgtg agcagcgttt ccctcaaaaa acgcccgctg gggatgacgg
    17701 gcagaaaatg cggcttgtcc accacaatca aatgctcatc aatatgcaaa atcttttctt
    17761 caaacggaat acgcggctca tcctcactgc tggtttcccg ataataaaac atcgtcttac
    17821 ccggctcgaa caaagaatgt tcgtccaacg ccgcaccatc cgaaccgacc acaaaaccgc
    17881 tgttcaaccg cctgcgccaa tcgtccgcgc ccacaaaagg aaagcggatg cacagaaaat
    17941 gcagcagcgg cagcccgtaa aactgctttt catgcggcag caccaaataa ctgggtttga
    18001 caccgttcaa cagcggaaga ggattattgc gttttttcat gcggcggatt gtaaccgttt
    18061 ttcacaaact tttcagacgg catatcaaaa cataattgat aaaaacaaac atattgtttt
    18121 ttatgattta aaaatttatt tagacaagat tatcggatat tcattattat cagtaaataa
    18181 gaattaatat caataggaga aatatgaagc gcatcttttt gcccgccttg cccgccatcc
    18241 tgcctttatc cgcttatgcc gacctgccct tgacgattga agacataatg accgacaagg
    18301 gcaaatggaa actgaaaact tcccttacct acctgaacag cgaaaacaac cgcgccgaac
    18361 ttgccgcacc ggtttacatc caaaccggcg caacctcgtt tatccccatt ccgaccgaaa
    18421 tccaagaaaa cggcagcaat accgatatgc tcgttggcac gctcggtttg cgctacggac
    18481 tgaccgggaa taccgacatt tacggcagcg gcagctatct gtggcacgaa gaacgcaaac
    18541 tcgacggcaa cggcaaaacc cgcaacaaac ggatgtccga cgtatccctc ggcatcagcc
    18601 acactttcct taaagacgac aaaaaccccg ccctaatcag ctttcttgaa agcacggttt
    18661 acgaaaaatc gcgcaacaaa gcctcgtcgg gaaaatcctg gctcatcggc gccaccacct
    18721 acaaagccat agatccgatt gtcctttccc tcaccgccgc ctaccgcatc aacggcagca
    18781 aaaccctttc agacggcatc cgctacaaat cgggcaacta cctgctgctc aaccccaaca
    18841 tctcatttgc cgccaacgac agaatcagcc tgaccggagg catccaatgg ctgggcacac
    18901 agcccgaccg catagacggc aaaaaagaaa ccgcaagaaa cacatccacc tacgctcatt
    18961 tcggcacagg tttcggtttc accaaaacca cggctttaaa cgcatccgca cgtttcaacg
    19021 tttcagggca aagcagttcc gaactgaaat ttggcgtaca gcatacattt taagcaggtt
    19081 ttgattttcc aaccttgtaa tccaaaggag ttattatgaa aaaacaaatc acctcagccg
    19141 taatgatgct gtctatgatt gcccccgcaa tggcaaacgg cttggacaat cagacatttg
    19201 aaaaccaagt gttccacacg caggcagatg cgccgatgca gttggcggag ctttctcaaa
    19261 aggagatgaa ggagactgag ggggagtgga ttcctaatgc tgttggaggc attatgggag
    19321 gagttggagg gcatttcagc tatatggcta gtgctattgc tagtggctcg tataatcgag
    19381 aggcacattg gacagcaatt ggaggtggtg cgttaattgg tgcgatgaac cctattaacg
    19441 gaggaagagc
//
LOCUS       DAWFHY010000037        19271 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.037, whole genome shotgun sequence.
ACCESSION   DAWFHY010000037 DAWFHY010000000
VERSION     DAWFHY010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 19271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19271
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.037"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(409..1488)
                     /gene="apbC"
                     /locus_tag="WGQ68_001122"
     CDS             complement(409..1488)
                     /gene="apbC"
                     /locus_tag="WGQ68_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HEZ0714927.1"
                     /translation="MNIQNIRTLLDTVAVPNTARTLGSEKAVRSVEQRSDGIHIALHF
                     GFPVAHIASETADRIQEILMPLTGDTHIHLSMDTEIGTHKVQPGVTTIKGVKNIIAVA
                     SGKGGVGKSTTTANLAAAMARMGARVGVLDADLYGPSQPTMLGVHDRKPDQKNQKLIP
                     VESSDGIQVMSIGFLVDTDQAVVWRGPMVSQALQQLMFQSEWDEVDYLFIDLPPGTGD
                     IQLTLSQRIPVTGSVIVTTPQDIALIDARKAVDMFRKVNIPILGVLENMSVHICTNCG
                     HSEALFGTDGGKDLAARLNLPLLGQLPLSLPVREAMDGGTPAQLFDEHPAIARIYTDA
                     AFQIALGIADKGKDFSSRFPKIVVE"
     gene            1699..1774
                     /locus_tag="WGQ68_001123"
     tRNA            1699..1774
                     /locus_tag="WGQ68_001123"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1732..1734,aa:Lys,seq:ttt)
     gene            1853..2362
                     /locus_tag="WGQ68_001124"
     CDS             1853..2362
                     /locus_tag="WGQ68_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein disulfide oxidoreductase"
                     /protein_id="HEZ0714928.1"
                     /translation="MKKKLLSGIKFAVQTALVFLLVSLFLDWVRKPEEPAGAAGRPLT
                     LLSGQRLTLGQFSRDRTVLVYFWGSWCGVCRYQSPIIDDLAADGVPVVGVAVRSGSSA
                     EVAAYMAKRGLGFPTVNDEDGGLARSWRIVATPAVVLVKNGKMVHYTTGISSYWGLRA
                     RIFQADVFG"
     gene            2705..3145
                     /gene="nadR"
                     /locus_tag="WGQ68_001125"
     CDS             2705..3145
                     /gene="nadR"
                     /locus_tag="WGQ68_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family adhesin repressor NadR"
                     /protein_id="HEZ0714929.1"
                     /translation="MPTQSKHASINISLIQAREALMTQFRPILNQANITDQQWRIIRL
                     LAENGTLDFQDLANQACILRPSLTGILTRLEKAGLVVRLKPSNDQRRVFLKLTAEGEK
                     LYEEIGEEVDERYDAIEEVLGREKMLLLKDLLAELAKIEDALNS"
     gene            3260..3760
                     /gene="hpaC"
                     /locus_tag="WGQ68_001126"
     CDS             3260..3760
                     /gene="hpaC"
                     /locus_tag="WGQ68_001126"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     reductase component"
                     /protein_id="HEZ0714930.1"
                     /translation="MADLQKTFQTSFRDAMASCAAGVHVITTDGAAGRYGITMTAVTP
                     VTDEPPTVMLCINRSARIIPILSENGSLCINTLADEHQDVAEHFAGLTGLSPEERFAY
                     HIWHRGKTGQLEIEGALAHLHGHIVGKHEIGTHFVFYVRLDEIKNCGCKRPALLYFRR
                     QFRFLD"
     gene            3776..4471
                     /locus_tag="WGQ68_001127"
     CDS             3776..4471
                     /locus_tag="WGQ68_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEZ0714931.1"
                     /translation="MKAMILAAGRGERMRPLTDTTPKPLLDVAGKPLIGWHLCRLKQA
                     GFTEIVINHAWLGRQIEDALGDGSAYGVNIAYSPEPAGGLETAGGIAQALPLLGDTPF
                     LVVNGDVLTDIDFTAAFQTASSLPEHISAHLWLVENPPHHPDGDFSLLPDGSVQSEVS
                     GGNGLTFSGVGIYRPEMFDGIEAGSVAKLAPVLRGEMRQNRVSGQKHTGLWLDVGTVC
                     RLKEAQALAGAWK"
     gene            complement(4717..5148)
                     /locus_tag="WGQ68_001128"
     CDS             complement(4717..5148)
                     /locus_tag="WGQ68_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0714932.1"
                     /translation="MSSNAWLFWALASAGFASLTAVFAKMGLQGIDSDFATFIRTLVI
                     LAALVLFLTYTGKWQGVNGFTGRNWTFLILSGLATGASWLAYFKALQLGNASQVAPID
                     KFSLVLVALMAVVFLDERPNTQEWIGLGLVTAGVLVLALKR"
     gene            5323..6999
                     /locus_tag="WGQ68_001129"
     CDS             5323..6999
                     /locus_tag="WGQ68_001129"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEZ0714933.1"
                     /translation="MSFKTDAEIAQSSTMRPIGEIAAKLGLNADNIEPYGHYKAKINP
                     AEAFKLPQKQGRLILVTAINPTPAGEGKTTVTIGLADALRHIGKDAVIALREPSLGPV
                     FGVKGGAAGGGYAQVLPMEDINLHFTGDFHAIGAANNLLAAMLDNHIYQGNELDIDPK
                     RVLWRRVVDMNDRQLRNIIDGMGKPVDGVMRPDGFDITVASEVMAVFCLAKDISDLKE
                     RLGNILVAYAKDGSPVYAKDLKAHGAMAVLLKDAIKPNLVQTIEGTPAFVHGGPFANI
                     AHGCNSVTATRLAKHLADYAVTEAGFGADLGAEKFCDIKCRLAGLKPDAAVVVATVRA
                     LKYNGGVERANLGEENLEALAKGLPNLLKHISNLKNVFGLPVVVALNRFVSDSDAELA
                     MIEKACAEHGVEVSLTEVWGKGGAGGADLARKVVNAIESQTNNFGFAYDVELGIKDKI
                     RAIAQKVYGAEDVDFSAEASAEIASLEKLGLDKMPICMAKTQYSLSDNAKLLGCPEGF
                     RITVRGITVSAGAGFIVALCGNMMKMPGLPKVPAAEKIDVDEHGVIHGLF"
     gene            7113..8204
                     /gene="ychF"
                     /locus_tag="WGQ68_001130"
     CDS             7113..8204
                     /gene="ychF"
                     /locus_tag="WGQ68_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002642527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEZ0714934.1"
                     /translation="MSLKCGIVGLPNVGKSTLFNALTQSGIEAANYPFCTIEPNVGIV
                     EVPDPRMVELAKIVNPQKMQPAIVEFVDIAGLVAGASKGEGLGNQFLANIRETDAIVN
                     VVRCFDDDNIVHVAGRVDPIADIETIGTELALADLASVEKAIVREEKRARSGDKDAQK
                     LVDLCKKLLPHLDEGKPVRSFGLDAEERAMLKPLFLLTAKPAMYVGNVSEDGFENNPH
                     LDHLKELAAKENAPVVAVCAAMESEIAELEDDEKAEFLAEMGLEEPGLNRLIRAGYDL
                     LGLQTYFTAGVKEVRAWTIHKGDTAPQAAGVIHTDFERGFIRAQVISYEDFIALGGEA
                     KAKEAGKMRVEGKEYVVQDGDVMHFLFNV"
     gene            8242..8541
                     /locus_tag="WGQ68_001131"
                     /pseudo
     CDS             8242..8541
                     /locus_tag="WGQ68_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010950985.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8530..8634
                     /locus_tag="WGQ68_001132"
                     /pseudo
     CDS             8530..8634
                     /locus_tag="WGQ68_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8645..10516
                     /locus_tag="WGQ68_001133"
     CDS             8645..10516
                     /locus_tag="WGQ68_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0714935.1"
                     /translation="MSQALPYRPDIDTLRAAAVLSVIVFHIEKNWLPGGFLGVDIFFV
                     ISGFLMTMILHREMSGGGGRFSLKAFYIRRIKRILPAFFAVLAATLTGGFFLFTKDDF
                     FLLWKSALTALGFASNLYFARGKDYFDPAQEEKPLLHIWSLSVEEQFYFVFPILLLLV
                     ARKSPRVQFGFLAALCALSLAASFMPSAFDKYYLPHLRACEMLVGSLTAVWMRCRQPA
                     VGRRCAAAGALFAVCILSTCLFSYSEQTAYFPGPAALIPCLAVAALIYFNHYEHPLKK
                     FFQWKITVAAGLISYSLYLWHWPILAFMRYIGPDNLPPYSPAAAIVLTLAFSLISYHC
                     IEKPFKKWKGSFAQSVLWIYALPMLILGAGSFFAMRLPFMAQYDRLGLTRSNTSCHNN
                     TGKQCLWGDTEKQPELLVLGDSHADHYKTFFDAVGKKEKWSATMVSADACAYVEGYAS
                     RVFQNWAACRAVYRYAEEHLPLYSKVVLAMRWGSQMPENSRSLAYDAGFFQKFDRMLH
                     KLSSEKQAVYLMADNLASSYNVQRAYILSSRIPGYRQTLRPDDESTLKANARIRELAA
                     KYPNVYIIDAAAYIPANFQIGGLPVYSDKDHINPYGGTELAKRFSEKQRFLDTRHNH"
     gene            10579..11874
                     /gene="tyrS"
                     /locus_tag="WGQ68_001134"
     CDS             10579..11874
                     /gene="tyrS"
                     /locus_tag="WGQ68_001134"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEZ0714936.1"
                     /translation="MSVIQDLQSRGLIAQTTDIEALDALLNEQKISLYCGFDPTADSL
                     HIGHLLPVLALRRFQQAGHTPIALVGGATGMIGDPSFKAAERSLNSAETVAGWVESIR
                     SQLTPFLSFEGGNAAIMANNADWFGKMNCLDFLRDIGKHFSVNAMLNKESVKQRIDRD
                     GAGISFTEFAYSLLQGYDFAELNKRHGAVLEIGGSDQWGNITAGIDLTRRLNQKQVFG
                     LTLPLVTKSDGTKFGKTEGGAVWLNAKKTSPYQFYQFWLKVADADVYKFLKYFTFLSI
                     EEIDAVEAKDQASGSKPEAQRILAEEMTRLIHGEEALAAAQRISESLFAEDQSSLTES
                     DFEQLALDGLPAFEVSDGINVVEALVKTGLASSNKEARGFVNSKAVLLNGKPAEANNP
                     NHAAERPDDACLLTDEHKRFGKYTILRRGKRNHALLVWK"
     gene            12401..13321
                     /gene="ribF"
                     /locus_tag="WGQ68_001135"
     CDS             12401..13321
                     /gene="ribF"
                     /locus_tag="WGQ68_001135"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEZ0714937.1"
                     /translation="MRIRLGRHNAPDFPHGAAVTIGNFDGVHLGHKHILQKLRLEADA
                     RGLPVVAVIFEPQPKEFFAALAGKTPPCRISPLRTKLELLEGTGCVDAVWVLRFDQNF
                     SEISAQGFIDRLLRQTLNTRYLLVGDDFRFGAGREGCFELLAQQPDMQTERTPSVIVE
                     DIRTSSTAVRQALSDGNLAYAKKLLGHDYVLSGRVVHGRKLGRTLNAPTANIRLPRHR
                     YALGGVFVVEADGAFGTRRGVASFGFNPTVDSDCFQKLEVHLFDFQGDLYGQRLNVRF
                     LHKLRDEEKFDGMEELKRQIEADMEAAKCW"
     gene            13462..16251
                     /gene="ileS"
                     /locus_tag="WGQ68_001136"
     CDS             13462..16251
                     /gene="ileS"
                     /locus_tag="WGQ68_001136"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEZ0714938.1"
                     /translation="MTDYSKTVNLLESPFPMRGNLAKREPAWLKSWYEQKRYQKLREI
                     AKGRPKFILHDGPPYANGDIHIGHAVNKILKDIIIRSKTQAGFDAPYVPGWDCHGLPI
                     EVMVEKLHGKDMPKARFRELCREYAAEQIARQKKDFIRLGVLGDWDHPYLTMDFKTEA
                     DTVRMLGEIYKSGYLYRGAKPVQFCLDCGSSLAEAEVEYKDKVSPAIDVAYPFKDTAA
                     LAAAFGLAGIEGKAFAVIWTTTPWTLPASQAVSVGADVVYQLIDTPKGKLVLAKDLAE
                     DALKRYGFSDGIAILAETTGDKLENLHMNHPFLERDIPMLNGDHVTTDAGTGLVHTAP
                     AHGLEDYAVCNKYGIELYNPVNAEGRYIGETPRVAGMRVWEANPVILQWLEETGNLLA
                     SSKIEHSYAHCWRHKTPLIYRATGQWFVGMDKAGADGKTLRDKAIKAVDDTEFFPSWG
                     RARLEAMIEGRPDWVVSRQRYWGTPMTFFVHKETGELHPNSAELLEKVALKIEEKGIE
                     AWFSLDKSELLSAEDCENYDKLSDTMDVWFDSGSTHYSVVKQREELEWPADLYLEGSD
                     QHRGWFQSSMLTGCASSMGRAPYKQLLTHGFVVDGEGKKMSKSIGNVVAPQEVYNEFG
                     ADILRLWAASTDYSGELAISKEILKRVTESYRRIRNTLSFLFANLSDFNPIEDAVQQA
                     DMVEIDRYALVLARRLQERLAGDYYPRYAFHFAVKEMVSFCSEDLGAFYLDILKDRLY
                     TTKADSRARRSAQTALYHITRSLVLLIAPILCFTGEEAWDIIGGGEEDSVLFHTWHEF
                     PTINEKTEAELVKKWTAIREAREAVTAAIEPLRADKTVGSSLQAEAEITAPEEMAGYL
                     NALGEELRFAMLVSKAEVKVGSELAVAAKASDGEKCERCWHYTRDVGAVAGYETVCKR
                     CAENVGGEGETRHYA"
     gene            17038..17532
                     /locus_tag="WGQ68_001137"
     CDS             17038..17532
                     /locus_tag="WGQ68_001137"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HEZ0714939.1"
                     /translation="MSSSVSSKTRYWVLALAAIVLDQWSKWAVLSSFQYRERVNVIPS
                     FFDLTLVYNPGAAFSFLADQGGWQKYFFLVLAVAVSAYLVRAILRDEFATLGKTGAAM
                     IIGGALGNVIDRLIHGHVVDFLLFYWQNWFYPAFNIADSFICVGAVLAVLDNIVHRKD
                     GKKT"
     gene            17562..18530
                     /gene="ispH"
                     /locus_tag="WGQ68_001138"
                     /gene_synonym="lytB"
     CDS             17562..18530
                     /gene="ispH"
                     /locus_tag="WGQ68_001138"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEZ0714940.1"
                     /translation="MNGKTIILANPRGFCAGVDRAISIVERALEEFGAPIYVRHEVVH
                     NKFVVDNLREKGAVFIEDLAEVPPGATLIYSAHGVSKAVQQEAAERGFRVFDATCPLV
                     TKVHKEVARLDAQDCEIIMIGHKGHVEVEGTMGQLAPGKMLLVETVGDVAKLEVRNPD
                     KLAYVSQTTLSVDETKDIIAALNARFPNIRNPHKEDICYATTNRQTAVKELAEQCDIV
                     IVVGSPNSSNSNRLREVAAGIGTDAYMVDNASYLQRAWFEGKNKVGVTAGASAPEVLV
                     QEVLATIRRWGHETVREGEGAEESIVFVLPKELRREGETKPDLCKR"
     gene            complement(18617..19174)
                     /locus_tag="WGQ68_001139"
     CDS             complement(18617..19174)
                     /locus_tag="WGQ68_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEZ0714941.1"
                     /translation="MGFGCREGKEFCKGLSLPEIIRDLLEMPSETAVADIARTYSAHY
                     LNPNNRNMTLFPDALPCLDKLKAQGYWLAVATGKGRAGLDGAISQTATGGYWLATACA
                     GEYPSKPSPEMVFGICRELGLDPKEALVVGDTAHDLHMAANAGAAAVGVTTGAHSREQ
                     LLSAPHLAVLDGLSELPGFLARHYA"
BASE COUNT         4408 a         5171 c         5344 g         4348 t
ORIGIN      
        1 ctagattccc gcctacgcgg gaatgacggg tcttttataa tctttgaata ttgctgttgt
       61 tctaaggtct agattcccgc ctgcgcggga atgacggttt agaagttgcc cgaaacctca
      121 aaaaaaaacc gaaaccgaac aagccggatt cccgcctgcg cgggaatgac ggcggagcgg
      181 tttctgtttt ttccgataaa tgccgcaatc tcaaatcccg tcattcccac gaacctacat
      241 tccgtcattc ccacgaaagt gggaatccag gacgcggaat ctcaagaaac cgtttacccg
      301 ataagtttcc gtgccgacag acctagattc ccgcctacgc gggaatgacg gcatatcggc
      361 aggcgcgggg cttgggacgg cattgctgtt gcggtttcgg acgcggcttt attcgacgac
      421 gattttaggg aaccggctgc tgaagtcttt gcctttgtcg gcaatgccca gggcgatttg
      481 gaatgcggca tcggtgtaga ttcgggcgat ggcggggtgt tcgtcgaaca gttgcgccgg
      541 tgtgccgccg tccatggctt cgcgcacggg caggcttagg ggaagctgtc cgagcagggg
      601 gaggttgagg cgtgcggcta aatctttgcc gccgtccgtg ccgaacagtg cttcgctatg
      661 cccgcagttg gtgcagatgt ggacggacat attttccaat acacctaaaa tgggaatgtt
      721 gacttttcgg aacatatcca cggctttgcg cgcgtctatc agggcgatgt cctgcggcgt
      781 ggttacgatg acggaaccgg ttacggggat gcgttgggac agcgtgagtt ggatgtcgcc
      841 cgtacccggg ggcaggtcga taaacaggta gtccacttcg tcccactcgc tttggaacat
      901 cagctgctgc aaggcttggc tgaccatcgg cccgcgccag acgacggctt ggtcggtatc
      961 gacgagaaag ccgatagaca tgacctgtat gccgtctgaa gattcgacgg gaatgagttt
     1021 ttggtttttc tgatcgggtt tgcggtcgtg tacgcccagc atcgtgggct ggctcgggcc
     1081 gtaaaggtcg gcatcgagta cgccgacgcg cgcgcccatg cgcgccattg cggcggcaag
     1141 gttggcagtg gtggttgatt tgcccacgcc gccttttccc gatgcgacgg cgatgatgtt
     1201 tttcacgcct ttgatggtgg taacgccggg ctggactttg tgtgtgccga tttcagtgtc
     1261 catggacaga tggatgtgtg tgtcgcctgt cagcggcatc aggatttcct gtatgcggtc
     1321 ggctgtttct gaggcaatgt gcgcgacggg gaagccgaaa tgcagggcga tatggatgcc
     1381 gtctgaacgc tgttcgaccg aacggacggc cttttcgctg ccgagcgtgc gtgccgtatt
     1441 cggaacggcg acggtgtcga ggagggtgcg gatgttttgt atgttcataa tgtgtgtttc
     1501 tgtgtgcagt ttgggaaacg ggcggaaagt gtacattgat ttcgggcggt ttgcttttcc
     1561 ttgtgcgaac ttttacacgg tatgcggcgg attgtgaaaa tctgttgcca aaatatcccg
     1621 tgcaaacagt ggtctgcaat taagcctgta aaatttgctt gacgaggcag gatggtgctg
     1681 tataatgcac ggcttatcgg gtcgttagct cagccggtag agcagcggac ttttaatccg
     1741 ttggtcgagc gttcgaatcg ctcacgaccc accagataac cggaagccaa gttttaagac
     1801 ttggcttttt gtttgcctgc cggttgcgcg ggcgtaccgt ttgagggggc gtatgaaaaa
     1861 gaaactgctt tcgggcatca aatttgccgt tcagacggca ttggtgtttt tattggtgtc
     1921 gctgtttttg gattgggtac gcaagcccga agagccggcc ggtgcggcag ggcggccttt
     1981 gaccctgctg tcggggcagc ggctgacttt gggacagttt agccgggata ggacggtgct
     2041 ggtgtatttt tggggaagct ggtgcggcgt gtgccgttat cagtcgccga taatcgatga
     2101 tttggcggcg gacggcgtgc cggtcgtcgg cgtggcggta cgttccggca gttcggcgga
     2161 agtggcggcg tatatggcaa aacgcggctt gggctttccg actgtcaacg atgaggacgg
     2221 gggtttggca aggtcttggc ggattgtcgc aacgcctgcc gtcgttttgg tcaaaaatgg
     2281 gaaaatggtc cattatacga cgggaatcag cagttattgg ggcttgcgcg cacgaatttt
     2341 tcaggcggat gttttcggtt aacttttgtt tttgttaaac tttacgctat gtagagaatc
     2401 aaacggcata taccttgctc gtctgatgcg gcggcttttg ttttttctgg aagaaagttc
     2461 tttcgacagt aaatgtaata ctttgttgag attgtaggga aaatagacta agatataact
     2521 attaaaatat ttttagatag gattatcgga attaaagtct tttataccca gtcgtccgat
     2581 gcggtttata gcgtattgtt gctatatgtt cgttttgtta tataacggtt gcatcaaaat
     2641 ttacgcccac aggctttccc gacggtttga aagtttgatt ttcgataact tggagacttg
     2701 aacaatgcct acccaatcaa aacatgcgtc tatcaatatc agtctgatac aggcaaggga
     2761 agccctgatg acccaattca ggcctattct gaatcaggcg aatattaccg atcagcaatg
     2821 gcggattatc cgtcttttgg cggaaaacgg cacgctggac tttcaagatt tggcgaatca
     2881 ggcgtgtatt ctgcgcccca gcctgaccgg tatcctgacc cgccttgaaa aagcgggttt
     2941 ggttgtccgc ctgaaacctt ccaacgacca acgccgcgtt tttctgaagc tgactgccga
     3001 gggcgagaag ctgtatgagg aaatcggcga agaagtggac gagcgttacg acgctatcga
     3061 ggaagtgctg ggccgcgaga aaatgctgct gcttaaagac ctgttggcag aacttgccaa
     3121 aatcgaggat gcgttgaact cgtaatacgc cgtaacgcgc ggaaacgtcc gaccgacggc
     3181 tttttgaatc aaaactgctg cacatggggg atgccttgtg tgcagcattc ttatataggg
     3241 gacagtttaa aggggaaaaa tggcggattt gcagaaaact tttcaaactt cgttccgtga
     3301 tgcgatggca tcttgtgcgg caggcgttca tgtcatcacg acagacggtg cggcagggcg
     3361 ttacggcatt acaatgacgg cggttacacc ggttacggac gagccgccga ccgtgatgct
     3421 gtgcatcaac cggagtgcgc gaatcattcc gatcctgtcg gaaaacggca gcctctgcat
     3481 caatacgctg gcggacgaac atcaggatgt tgccgaacat tttgccgggc tgaccgggct
     3541 gtcgcccgaa gagcggtttg cctaccacat ctggcatcgc ggcaaaacgg gacaacttga
     3601 aatagagggc gcgttggcgc acctgcacgg gcatattgtc ggcaaacatg aaatcggcac
     3661 gcattttgtg ttttacgtca ggctcgacga aatcaaaaac tgcgggtgca aacgccccgc
     3721 gctgctgtat ttcagacggc agtttagatt tttagactga tattcggaca gatatatgaa
     3781 agcgatgata ctggcggcag gacgcggcga gcgtatgcgc cctttgaccg ataccactcc
     3841 gaagccgctg ctcgatgtgg cgggtaagcc tctaatcggt tggcacctat gccgtctgaa
     3901 gcaggcgggg tttaccgaaa tcgtcatcaa ccacgcttgg ctgggccggc agatagaaga
     3961 tgctttgggc gacggctcgg cttatggcgt gaacatcgcc tattcgcccg aacccgcagg
     4021 cggtttggaa acggcaggcg gcatcgcgca ggcattgccg ctgttgggcg ataccccgtt
     4081 tttggtcgtc aatggcgacg tgctgaccga catcgatttt accgccgcgt ttcagacggc
     4141 atcgtccctg ccggaacata tttccgccca tctgtggctg gtggaaaatc cgccgcacca
     4201 tcctgacggc gatttttccc tgctgccgga cggcagcgta cagtcggaag tatccggcgg
     4261 caacggattg acatttagcg gcgtgggcat ttaccgtcct gaaatgtttg acggaatcga
     4321 agcgggcagt gtggcgaaac tcgcgcccgt attgcgtggc gaaatgcggc aaaaccgcgt
     4381 gagcggtcag aagcatacgg gcttgtggct ggatgtcggc acggtatgcc gtctgaaaga
     4441 ggctcaagcc cttgcagggg cttggaagtg aaataaaacg gtggcttgcc gagccggctg
     4501 ccgaacggtt ccgaccggat gtccggcata ttttcgtgga ggcgtgaaaa tagggcgttc
     4561 ggggctttcc gtccaacaaa tgccatctga agccgacccc tgtcggatgc ggcggtttgc
     4621 cgtcggcgcg gtgtcggatt ttattgggta actttgttca gaccgccgcc cgtcagattg
     4681 tcaaaacccg gtttcaggcg gtatggcgga ttcggtttaa cgtttcaacg ccagcaccaa
     4741 cacgcccgcc gttaccagcc ccaagcctat ccattcctgc gtgttcgggc gttcgtccaa
     4801 gaaaaccacc gccatcagcg cgaccaagac caggctgaat ttgtcgatgg gggcgacttg
     4861 cgaggcgttg cccagttgca gggctttgaa gtaggcgagc caggatgcgc cggtagcaag
     4921 gccggatagg ataaggaatg tccagttgcg ccccgtaaag ccgttcacac cctgccattt
     4981 gccggtgtag gtcaaaaaca ataccaaagc ggcgaggatc accaaggtgc ggataaaggt
     5041 ggcgaaatcg gaatcgatgc cctgtaaacc cattttggcg aaaacggcgg tcaatgaggc
     5101 gaagcctgcc gatgccaatg cccaaaacag ccatgcgttg ctgctcatgt tttctccttg
     5161 gattgtgaac aatatgaacg gtatttttgt tgcagcgtca aaaatttcac tgcgggtttg
     5221 gtgcggataa cgttataata tgcctgatat tattttcaat ccacctgttt gtcgcctgat
     5281 gctttcagac ggcatgtccc tcctcatttc taaaggaaaa tcatgagctt caaaaccgat
     5341 gccgaaatcg cccaatcctc caccatgcgc ccgattggcg aaattgccgc caagcttggt
     5401 ctgaatgccg acaacattga gccttacggt cattacaagg cgaaaatcaa tcctgccgaa
     5461 gcgttcaaac tgccgcaaaa acagggcagg ctgattttgg ttaccgccat caacccgact
     5521 ccggcgggcg aaggcaaaac caccgtaacc atcggtttgg cggacgcgtt gcgccacatc
     5581 ggcaaagatg ccgtgattgc cctgcgcgaa ccttctctgg ggccggtgtt cggcgtgaaa
     5641 ggcggcgcgg caggcggcgg ctatgcccaa gttttgccga tggaagacat caacctgcac
     5701 ttcaccggag attttcacgc catcggtgcg gcaaataatc tgcttgccgc gatgctcgac
     5761 aaccatatct atcagggcaa cgagttggac atcgacccca aacgcgtgct gtggcggcgc
     5821 gtggtcgata tgaacgaccg ccagttgcgc aacatcatcg acggcatggg taaacccgtt
     5881 gacggtgtga tgcgccccga cggttttgat atcaccgttg cctccgaagt gatggcggta
     5941 ttctgccttg ccaaagacat cagcgatttg aaagagcgtt tgggcaacat cctcgtcgcc
     6001 tacgccaaag acggcagccc cgtttacgcc aaagatttga aggcacacgg cgcgatggcg
     6061 gtcctgctca aagatgcgat taagcccaat ttggtgcaaa ccatcgaagg cacgcccgcc
     6121 ttcgtacacg gcggcccgtt cgccaacatc gcccacggct gcaactccgt aaccgcaacc
     6181 cgtctggcga aacaccttgc cgattacgcc gtaaccgaag caggcttcgg cgcggatttg
     6241 ggcgcggaaa aattctgcga catcaaatgc cgccttgccg gtttgaaacc tgatgcggcg
     6301 gtcgtcgtgg cgactgtccg cgctttgaaa tacaacggcg gcgtggaacg tgccaacctc
     6361 ggtgaagaaa acctcgaagc cttggcaaaa ggtttgccca acctgctgaa acacatttcc
     6421 aacctgaaaa acgtattcgg actgcccgtc gtcgttgcgc tcaaccgctt cgtgtccgac
     6481 tccgatgccg agttggcgat gattgaaaaa gcctgtgccg aacacggcgt tgaagtttcc
     6541 ctgaccgaag tgtggggcaa aggcggcgcg ggcggcgcgg atttggcgcg caaagtcgtc
     6601 aacgccattg aaagtcaaac caataacttc ggtttcgcct acgatgtcga gttgggcatc
     6661 aaagacaaaa tccgtgcgat tgcccaaaaa gtgtacggcg cggaagatgt tgatttcagc
     6721 gcggaagcgt ctgccgaaat cgcttcactg gaaaaactgg gcttggacaa aatgccgatc
     6781 tgcatggcga aaacccaata ctctttgagt gacaacgcca aactgttggg ctgccccgaa
     6841 ggcttccgca ttaccgtgcg cggtatcact gtttccgcag gcgcaggttt catcgtcgcc
     6901 ctgtgcggca acatgatgaa aatgcccggc ctgcccaaag ttccggctgc cgagaaaatc
     6961 gatgtggacg aacacggcgt gattcacggc ttgttctgaa cggtttttga aaccagatgc
     7021 cgtctgaagc cgtttcagac ggcatttttt cggaacgcgg gcggcggtat gctataatcc
     7081 gccgttaaat ttctctattt tcaggaaaaa acatgagttt gaaatgcggc atcgtcggtt
     7141 tgcccaacgt cggcaaatcc accctcttta acgcgctgac ccagtcgggt atcgaagcgg
     7201 caaactatcc tttctgtacc atcgaaccca acgtcggcat cgtcgaagtg cccgacccgc
     7261 gtatggtcga attggcaaaa atcgtcaatc cgcaaaaaat gcagcctgcc atcgtcgaat
     7321 ttgtcgatat tgccggtttg gtcgcgggcg cgagcaaagg cgagggcttg ggcaaccagt
     7381 tccttgccaa catccgcgaa accgacgcga ttgtgaatgt cgtgcgctgc tttgacgacg
     7441 acaatatcgt ccacgttgca ggccgcgtcg atccgattgc cgacatcgaa accatcggta
     7501 cagagttggc gcttgccgac ttggcaagtg tggaaaaagc catcgtccgc gaagaaaaac
     7561 gcgcccgctc aggcgacaaa gacgcgcaaa agctggtcga tttgtgcaaa aaactgctgc
     7621 cgcatctgga cgaaggcaaa cccgtgcgtt ccttcggttt ggacgcggaa gaacgcgcga
     7681 tgctcaagcc gctgttcctg ctgaccgcta aacccgcgat gtatgtgggc aacgtttccg
     7741 aagacggttt tgaaaacaat ccgcacctcg accacctgaa agaattggct gcaaaagaaa
     7801 acgcccccgt cgtcgccgta tgcgccgcga tggaaagcga aatcgccgaa ttggaagacg
     7861 acgaaaaagc cgagttcctc gccgaaatgg gcttggaaga accaggcctg aaccgcctga
     7921 tccgcgcggg ctacgacctc ttaggcctgc aaacctactt caccgccggc gtgaaagaag
     7981 tccgcgcttg gaccatccac aaaggcgaca ccgccccgca agccgccggc gtgattcaca
     8041 ccgactttga acgtggcttc atccgcgccc aagttatctc ctacgaagac ttcatcgctt
     8101 taggcggcga agccaaagcc aaagaagccg gcaaaatgcg tgtggaaggc aaagaatacg
     8161 tcgtgcagga tggggatgtg atgcactttt tgtttaacgt gtaacccaaa tgcggcaggt
     8221 tttcagacgg ctttgccgga aatgccgtct gaagccggtt ttggtggttt tcggcgtttc
     8281 cataccgccg gaatgcagcc gtatcaaaat atagcggatt aacaaaaatc aggacaaggc
     8341 ggggcaacgc tgtaccggtt tttgttaatc cactataaaa tcccacccgc atttccgatt
     8401 tgccctcctc gattcctgca aaacaaaccg cctgccccgc tgttgcaaaa agccgtccgg
     8461 cattccgaat atcccgagcc ccgatacaaa atgacctttc agacggcatt tgcgccgccg
     8521 cgtttcgagt atagtggatt aactttaaat caggacaagg cgacgaaacc gcagacagta
     8581 cagatagtac ggcaaggcga ggcaacgccg tactggttta aatttaatcc actataaaaa
     8641 cattatgagc caagccttac cctaccgccc ggacatcgac acattgcgcg ccgccgccgt
     8701 cttgtccgtc atcgtgttcc atatcgaaaa aaattggctg ccgggcgggt ttctcggtgt
     8761 cgatatattc tttgtgattt caggcttttt gatgacgatg attcttcacc gcgaaatgtc
     8821 gggggggggg gggaggtttt ccctgaaggc attttatatc cgccgcatca agcggattct
     8881 gcccgcattt ttcgccgtat tggcggcaac gctgacaggc ggcttctttt tattcaccaa
     8941 agatgatttc tttcttttat ggaaatccgc gctgaccgcc ttgggtttcg cctccaacct
     9001 gtattttgca agggggaagg attatttcga tcccgcgcag gaagaaaagc ccttgctgca
     9061 catttggtct ttgtcggtcg aagaacaatt ttactttgtc tttccgatat tgctgctgct
     9121 tgtcgcccgc aaaagcccgc gcgtacagtt cggcttcctc gccgcattgt gcgccttaag
     9181 ccttgccgct tcctttatgc cttccgcgtt cgataaatat tacctgcccc acctgcgcgc
     9241 ctgcgaaatg ctggtcggat cgctgaccgc cgtgtggatg cgctgccggc aacctgccgt
     9301 cggcagacgc tgtgccgccg ccggcgcatt gtttgccgtg tgcatattgt caacctgcct
     9361 gttttcctat tcggaacaaa ccgcctattt cccgggcccc gccgctttga ttccctgtct
     9421 ggctgttgcc gcgctgattt atttcaatca ttacgaacac ccgcttaaaa aatttttcca
     9481 atggaaaatc accgttgccg ccggtttgat ttcctattcg ctttatctgt ggcattggcc
     9541 gatattggcc tttatgcgct atatcggccc ggacaacctg ccgccttatt cgccggcggc
     9601 agcgatcgtc ctgactctag cgttttccct gatttcttat cactgcatcg aaaagccgtt
     9661 taaaaaatgg aaaggttcgt tcgcacaatc cgttttatgg atttatgcct tgcctatgct
     9721 cattttgggg gcgggctcgt ttttcgcgat gaggctgccg tttatggcgc aatacgaccg
     9781 cttggggctg acgcgttcca acacctcctg ccacaacaat accggcaaac aatgcctatg
     9841 gggggatacg gaaaaacagc cggaactgct ggttttgggc gattcccacg ccgaccatta
     9901 caaaacattc ttcgatgccg tgggcaaaaa agaaaaatgg tccgccacta tggtttccgc
     9961 cgacgcttgc gcctatgtgg aaggctacgc gtcccgtgtg ttccaaaact gggccgcctg
    10021 ccgcgccgtt taccgctatg ccgaagaaca cctgcccctg tattcaaaag tggttttggc
    10081 gatgcgctgg ggcagccaaa tgcccgaaaa cagccgctcc cttgcctacg atgccggttt
    10141 tttccaaaaa ttcgaccgta tgctgcacaa actctcatcc gaaaaacaag ccgtttacct
    10201 gatggcggac aaccttgcct cgtcttacaa cgtacaacgc gcctatatct tgtcttcacg
    10261 cataccgggt taccgccaaa ccctgcgccc ggacgacgaa agcactctga aagccaatgc
    10321 acgcatcagg gaattggcag ccaaataccc caacgtctat attattgatg ccgccgccta
    10381 tatccccgca aatttccaaa tcggcggatt gccggtttac tcggacaaag accacatcaa
    10441 cccttacggc ggcacggaat tggcgaagcg tttttccgaa aaacaacggt ttctcgatac
    10501 gcgccataac cattgattcg cttaaatttg ttacaatcgg cggtttgcaa aaccgctaat
    10561 tttttttgaa agagaccgat gagcgtcatc caagacctgc aatcgcgcgg ccttatcgcg
    10621 caaaccaccg acatcgaagc cttagacgct ttgttgaacg aacaaaaaat ttccctttat
    10681 tgcggcttcg acccgaccgc cgacagcctg cacatcggac acctgctgcc cgtattggca
    10741 ttgcgccgct tccaacaggc ggggcatacg ccgattgcac tggtgggcgg tgcgaccggt
    10801 atgatcggcg accccagctt caaagccgcc gaacgcagct tgaattccgc cgaaaccgtt
    10861 gccggctggg tggaaagcat ccgcagccaa ttaacccctt tcttgagctt tgaaggcgga
    10921 aacgccgcca ttatggcgaa caacgccgac tggttcggca aaatgaactg cctcgacttc
    10981 ctgcgcgaca tcggcaagca tttctccgtc aacgccatgc tgaacaaaga atccgtcaaa
    11041 cagcgcattg accgcgacgg cgcaggcatt tccttcaccg agttcgccta ctccctgctg
    11101 caaggctacg acttcgccga gttgaacaaa cgccacggcg cggttttgga aatcggcggc
    11161 tccgaccaat ggggcaacat caccgccggt atcgacctga cccgccgcct gaatcaaaaa
    11221 caagtgttcg gcctgaccct gcctttggtt accaaatcag acggcaccaa attcggcaaa
    11281 accgaaggcg gcgcagtatg gctgaacgcg aaaaaaacct cgccctatca gttctaccaa
    11341 ttctggctga aagtcgccga tgccgatgtg tataaattcc tgaaatactt taccttcctg
    11401 tccatcgaag aaatcgatgc cgtcgaagcc aaagaccagg caagcggcag caagcccgaa
    11461 gcgcaacgca tcctcgccga agaaatgacc cgcctgattc acggcgaaga agcccttgcc
    11521 gccgcgcagc gcatttccga aagcctgttt gccgaagacc aaagcagcct gaccgaaagc
    11581 gacttcgagc agctcgccct cgacggcctg cctgcatttg aagtttcaga cggcatcaat
    11641 gtcgtcgaag ccttggtcaa aaccggtttg gcatcctcca ataaagaagc gcgcggcttt
    11701 gtgaacagca aagcggtttt gctcaacggc aaacctgccg aagccaacaa ccccaaccac
    11761 gccgccgaac gccccgacga tgcctgcctg ctgaccgacg aacacaaacg tttcggcaaa
    11821 tacaccatcc ttcggcgcgg caaacgcaat cacgcgcttt tggtttggaa ataatccgat
    11881 tgccgcagaa atgccgtcat tcccgcgcag gcgggaatcc ggacctgtcc gcacggaaac
    11941 ttatcgggca aaacggtttc ttagattcca cgttctagat tcccgcctga gcgggaatga
    12001 cgagtttcaa gattacggtg ttgtcggaac gcaactgaac cgtcattccc acgaaagtgg
    12061 gaatctagaa tctcggggtt tgagcaactg tttttatccg ataagtttct gtgcggacag
    12121 gtccggattc ccgcctgcgc gggaatgacg gcggagggtt gtttgtctcg gtttacctgg
    12181 ttaaaaaaga acgattttca ctgatgttgc atcaggtttg gggcgatgtt tcaacacata
    12241 gcaccgcgcc tgctgcgcgc tttatgcgtt tggcgcgttc ggctgcggga aatttgccta
    12301 cttttcccgc gtcgggcggg cgtaacgggc ggcacactgt ctataaaccg caataccgtt
    12361 tacaatgacc gcctgtttga ccacataccc gaacataaca atgagaatca ggctggggcg
    12421 gcacaacgcg cccgactttc cgcacggggc cgccgtaacc ataggcaatt tcgacggcgt
    12481 acacctcgga cacaaacaca tcctccaaaa actccgcctc gaagccgacg cgcgcggact
    12541 gcccgtcgtt gccgtcatct tcgaacctca acccaaagaa ttttttgccg ccctcgcagg
    12601 caaaaccccg ccctgccgca tcagccccct gcgtaccaaa ctggaattgc tggaagggac
    12661 gggctgcgtc gatgccgtct gggttttgcg tttcgatcaa aatttttccg aaatatccgc
    12721 gcaagggttt atcgaccgcc tgctgcgtca aaccttgaat acgcgttatt tgctcgtcgg
    12781 cgacgatttc cgtttcggtg cggggcggga aggctgtttt gaacttttgg cacaacagcc
    12841 cgatatgcag accgagcgta cgccttccgt catcgtcgaa gacatccgca ccagcagtac
    12901 cgccgtgcga caagcccttt cagacggcaa ccttgcctat gcgaaaaaac ttttgggaca
    12961 cgactacgtc ttgagcggca gggtggtgca cggcagaaaa ctcggacgca ccttaaacgc
    13021 cccgactgcc aacatccgcc tgccccgcca ccgttatgca ctcggcggcg tgttcgtcgt
    13081 cgaagcggac ggcgcgttcg gcacgcggcg cggcgtggcg agcttcggct tcaatcccac
    13141 cgttgatagc gactgttttc aaaagctgga agtccacctg ttcgactttc aaggcgattt
    13201 gtacggacaa cggttgaacg tccgcttcct gcacaaactg cgcgatgagg aaaagtttga
    13261 cggtatggaa gaactgaaaa ggcagattga agccgatatg gaagccgcaa agtgttggta
    13321 gaaaaacctt atacaaacca tccgattggg ctacaatcaa ccttttaact gttcagacgg
    13381 cataggggtt tcccgttgtg aaatactgtt tgaggcgcaa tgccgtctga accgaaatat
    13441 tgtaacaata gagattaaaa aatgaccgat tacagcaaaa ccgtcaacct gctcgaaagc
    13501 ccgtttccga tgcgcggcaa tcttgccaag cgcgagcctg catggctgaa aagctggtac
    13561 gagcaaaaac gctaccaaaa actgcgcgaa atcgccaaag gccgtccgaa attcattctg
    13621 cacgacggcc cgccgtatgc caacggcgac atccacatcg gtcatgccgt caataaaatt
    13681 ctcaaagaca tcattatccg cagcaaaacc caagccggtt ttgacgcgcc ttatgtgccg
    13741 ggttgggact gccacggcct gcccatcgaa gtgatggtag aaaaactgca cggcaaagat
    13801 atgcccaaag cacgtttccg cgaattgtgc cgcgaatacg ccgccgaaca gattgcccgt
    13861 cagaaaaaag actttatccg cttgggcgtg ttgggcgact gggaccatcc ttacctgact
    13921 atggatttca aaaccgaagc cgataccgtg cgtatgctcg gcgaaatcta caaatccggc
    13981 tatctctacc ggggtgcgaa accggttcaa ttctgcttgg actgcggttc ttcattggcc
    14041 gaagcggaag tggaatacaa agacaaagta tcgcctgcga ttgacgttgc ctatccgttt
    14101 aaagacaccg ccgcgcttgc cgccgcattc ggtttggcag gtatcgaagg caaagcgttt
    14161 gccgtcatct ggacgaccac gccttggacg ctgccggcga gccaagccgt atctgtcggc
    14221 gcggacgtgg tgtatcaatt aatcgatacg cccaaaggca aattggtgct ggcgaaagat
    14281 ttggcggaag acgctttgaa acgctacggc ttttcagacg gcatcgccat ccttgccgaa
    14341 accaccggcg acaagctgga aaacctgcat atgaaccatc cgtttttgga acgcgatatt
    14401 cctatgctca acggcgacca cgttactacc gatgccggta ccggcttggt acacaccgcc
    14461 cccgcgcacg gtttggaaga ctacgccgtc tgcaataaat acggcatcga gctttacaac
    14521 cccgtcaacg ccgaaggccg atacatcggc gaaacgccgc gtgtcgccgg tatgcgcgtt
    14581 tgggaggcga accccgtcat cctgcaatgg ttggaagaaa ccggcaacct tttggcaagc
    14641 agtaaaatcg aacacagcta cgcccactgc tggcggcaca aaacgccgct gatttaccgc
    14701 gcgacaggtc aatggtttgt cggtatggac aaagccggtg ccgacggcaa aaccctgcgc
    14761 gacaaagcca tcaaggccgt ggacgacacc gaattcttcc cgtcttgggg tcgcgcgcgt
    14821 ttggaagcca tgattgaagg tcgtcctgac tgggtggttt cacgccaacg ctattggggc
    14881 acgccgatga ctttctttgt tcacaaagaa acgggcgagc tgcatccgaa ctctgccgaa
    14941 cttttggaaa aagttgccct gaaaatcgaa gaaaaaggca tcgaagcgtg gttctccctc
    15001 gataagagcg aactcttgag cgcggaagat tgcgaaaatt acgataaact ttctgacaca
    15061 atggacgtat ggttcgactc tggctcgacc cattattccg ttgtgaaaca acgcgaagaa
    15121 ttggaatggc cggctgattt gtatctcgaa ggcagcgacc aacaccgcgg ctggtttcaa
    15181 tcgtccatgc tgaccggctg cgcctcatca atgggtcgcg caccgtataa acagctgctg
    15241 acccacggtt ttgttgtaga cggcgaaggc aaaaaaatgt cgaaatccat cggcaacgtc
    15301 gtcgcgccgc aagaagtcta taacgaattc ggcgcggaca tcctgcgtct gtgggcggca
    15361 tctaccgatt acagcggcga attggcgatt tctaaagaaa tccttaagcg cgtaaccgaa
    15421 agctaccgcc gtatccgcaa taccttgagc tttttgtttg ccaacttgag cgactttaat
    15481 ccgattgaag atgccgtgca acaggcggat atggtggaaa tcgaccgcta tgccctggtg
    15541 ttggcgcgtc ggctgcaaga gcgtctggca ggcgattact atccgcgtta tgccttccat
    15601 tttgccgtga aagaaatggt ttctttctgc tcggaagact tgggcgcgtt ctacctcgat
    15661 atcctgaaag accgcctcta caccaccaaa gccgacagcc gcgcccgccg cagcgcgcaa
    15721 accgccctgt accacatcac gcgcagcctg gttctcttga ttgcaccgat tttgtgcttt
    15781 accggcgaag aagcgtggga catcatcggc ggcggcgaag aagacagcgt cctcttccat
    15841 acttggcacg agttcccgac catcaacgaa aaaaccgaag ccgaactggt gaaaaaatgg
    15901 acggcaatcc gcgaagcccg cgaagcggta accgccgcca tcgagccttt gcgcgccgac
    15961 aaaaccgtcg gttcgtcctt gcaagccgaa gccgaaatca ccgcgccgga agaaatggcc
    16021 ggctacctga acgccttggg cgaagaattg cgctttgcca tgctggtgtc taaagcagaa
    16081 gtgaaagtag gcagcgaact tgccgttgcc gctaaagcca gtgatggtga aaaatgcgaa
    16141 cgctgctggc actacacccg cgatgtgggc gcggttgcag gctatgaaac cgtctgcaaa
    16201 cgctgtgcag agaatgtcgg cggagaaggc gaaacgcgcc attacgcctg ataaagtttg
    16261 agcaaatgcc gtctgaaacc gccgaacagc atttcagacg gcattttttg tgccgcgatt
    16321 tgtctttata acggcggagg ggtttcaaga ttgcggtgtt gtcggaatgc aactgaaccg
    16381 tcattcccac gaaagtggga atctagaatc tcgaggtttc agtcatttcc gatagattcc
    16441 cgccgcgtcg tgggtctgga ttcccgcctg tgcgggaatg acggatttcg agattgcggt
    16501 gttgtcggaa cgcaactgaa ccgtcattcc cacgaaagtg ggaatctaga atctcggggt
    16561 ttcagtcatt tccgatagat tcccgccgcg tcaggggtct ggattcccgc ctgtgcggga
    16621 atgacggatt tcgagattac ggtgttgtcg gaacgcaact gaaccgtcat tcccacgaaa
    16681 gtgggaatct agaatctcgg ggtttcagtc atttccgata gattcccgcc gcgtcggagg
    16741 tctggattcc cgcctgcgcg ggaatgacgg atttcgagat tgcggtgttg ttggaacgca
    16801 actgaaccgt cattcccacg aaagtgggaa tctagaacga ggggtttgag caactgtttt
    16861 tatccgataa gtttctgtgc ggacaggtct ggattcccgc ctgcgcggga atgacgggtt
    16921 tcgagattac ggtgttgtcg ggaatgacgg gtttcaagat tccggcattt gccgtttcgg
    16981 gtacaggcag ggggttttcg ggtaaaatgg cactctttta tccggctgtt gaaaaatatg
    17041 tcttcatctg tttcaagtaa aacgcgctat tgggtattgg cacttgccgc catcgtgctg
    17101 gaccagtggt cgaagtgggc ggtgctgtcg tcgtttcagt atcgggaacg cgtcaatgtc
    17161 attccttcgt ttttcgatct gaccttggtg tacaacccgg gcgcggcgtt cagcttcctt
    17221 gccgatcagg gcggctggca aaaatacttt tttttggtgc tggcggtggc ggtgagcgcg
    17281 tatttggtac gcgccatctt gcgcgatgag tttgcaaccc tcggcaaaac gggtgcggcg
    17341 atgattatcg gcggtgcgtt aggcaatgtc atcgatcgcc tgatacacgg tcatgtcgtc
    17401 gatttcttat tgttttattg gcaaaattgg ttttatcccg cctttaatat tgccgacagc
    17461 tttatctgcg tcggtgcggt gttggcggtg ttggacaata tcgtccatcg caaagatggc
    17521 aaaaaaacgt gaatgccgtc tgaacacgga atgtaaaact tatgaacgga aaaaccatca
    17581 tccttgccaa tccgcgcggc ttctgcgccg gcgtggatcg ggcaatcagt attgtcgagc
    17641 gtgctttgga agaattcggc gcgccgattt atgtgcgcca cgaagtcgtc cacaacaaat
    17701 tcgtcgtcga taacctgcgt gaaaaaggcg cggtgtttat cgaagacttg gcggaagtgc
    17761 cgccgggcgc gacgctgatt tattcggcac acggtgtatc gaaggcggtg cagcaggaag
    17821 cggcggagcg cggtttccgc gtgtttgatg cgacttgccc gctggttacg aaagtgcata
    17881 aggaagtcgc ccgactggat gcccaagact gtgaaatcat catgatcggg cataaggggc
    17941 acgttgaggt cgaaggaacg atggggcagc ttgcgccggg caaaatgctt ttggtcgaaa
    18001 cggtcggaga tgtggcaaaa ctcgaagtca gaaaccccga caaactcgcc tatgtcagcc
    18061 aaaccacgct ctcggtcgat gaaaccaaag acatcattgc cgcgctgaac gcgcgtttcc
    18121 ccaatatccg caatccgcac aaggaagaca tctgctatgc gacgaccaac cggcaaaccg
    18181 ccgtcaaaga gctggcggaa cagtgcgaca tcgtgattgt ggtcggttcg cccaattcgt
    18241 ccaacagcaa tcgcctgcgc gaagtggcgg caggcatcgg gaccgatgcg tatatggtgg
    18301 acaacgcgtc ctacctgcaa cgcgcgtggt ttgagggcaa aaacaaagtc ggcgtaacgg
    18361 caggcgcgtc cgcgcccgaa gtgttggtgc aggaagtact ggcaaccata cgcagatggg
    18421 ggcacgaaac cgtacgcgaa ggggagggcg cggaagaaag cattgtgttc gtcctgccca
    18481 aagagctgcg ccgcgagggc gaaaccaaac ccgatttgtg caaacgttga gcaagcgtcg
    18541 gatgtttggg caacacaaat gccgtctgaa caggcttcag acagcatttt tgccgcgtgc
    18601 cggatgcgga aaccgatcag gcgtaatgtc gtgcaagaaa accgggcagt tcggacaaac
    18661 cgtccaatac ggcgagatgc ggtgcgctaa ggagctgttc gcgcgaatgt gcgccggtgg
    18721 taacgccgac tgccgccgca cccgcgtttg ccgccatatg caggtcgtgc gccgtatcgc
    18781 cgacgaccaa tgcctctttc gggtcgagtc ccagttcgcg gcagattccg aacaccattt
    18841 cgggcgaggg tttggaggga tattcccccg cgcaggcggt ggcgagccaa tagccgccgg
    18901 tggcggtttg gctgatggca ccgtccaagc ccgcccgccc tttgcccgtg gcgacggcaa
    18961 gccagtatcc ttgtgctttg agcttgtcca gacagggcag ggcatcggga aagagcgtca
    19021 tattgcggtt gttgggattg aggtaatgcg cggaataagt gcgcgcgatg tcggcaacgg
    19081 cggtttcaga cggcatttcg agcaggtcgc ggatgatttc gggcaggctg agacctttgc
    19141 aaaattcctt tccctcccga cagccgaaac ccaaacacag gttttcggct gttttcgccc
    19201 caaataccgc ctaattctac ccaaataccc ccttaatcct ccccggatac ccgataatca
    19261 ggcatccggt c
//
LOCUS       DAWFHY010000038        18408 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.038, whole genome shotgun sequence.
ACCESSION   DAWFHY010000038 DAWFHY010000000
VERSION     DAWFHY010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18408
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.038"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(152..1084)
                     /gene="cysK"
                     /locus_tag="WGQ68_001140"
     CDS             complement(152..1084)
                     /gene="cysK"
                     /locus_tag="WGQ68_001140"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEZ0714942.1"
                     /translation="MKIANSITELIGNTPLVKLNRLTEGLKAEVAVKLEFFNPGSSVK
                     DRIAEAMIEGAEKAGKINKNTVIVEATSGNTGVGLAMVCAARGYKLAITMPESMSKER
                     KMLLRAFGAELILTPAAEGMAGAIAKAKSLVDAHPDTYFMPRQFDNEANPEVHRKTTA
                     EEIWRDTDGKVDVFVAGVGTGGTITGVGEVLKKYKPEVKVVAVEPEASPVLSGGEKGP
                     HPIQGIGAGFIPTVLNTKIYDSITKVPNEAAFETARAIAEKEGILVGISSGAAVWSAL
                     QLAKQPENEGKLIVVLLPSYGERYLSTPLFADLA"
     gene            complement(1278..1883)
                     /locus_tag="WGQ68_001141"
     CDS             complement(1278..1883)
                     /locus_tag="WGQ68_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714943.1"
                     /translation="MILLHLDFLSALLYAAVFLFLIFRAGMLQWFWASIALWLGISVL
                     GAKLMPGIWGMTRAAPLFIPHFYLTLGSIFFFIGHWNRKTDGNGWQADPEHPLLGLFA
                     VSNVSMTLAFVGICALVHYCFSGTVQVFVFAALLKLYALKPVYWFVLQFVLMVLAYVH
                     RCGIDRQPPSTFGGSQLRLGVLAAALMQVSVLVLLLSEIGR"
     gene            complement(1880..2731)
                     /gene="dapF"
                     /locus_tag="WGQ68_001142"
     CDS             complement(1880..2731)
                     /gene="dapF"
                     /locus_tag="WGQ68_001142"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEZ0714944.1"
                     /translation="MNTLKFTKMHGLGNDFMVIDAVSQDFTPEDAPIAAWADRFRGVG
                     FDQLLVVGRSETEGVDFRYRIFNADGSEVGQCGNGARCFARFVADKGLTDKKEICVET
                     ANGVIFPKLSDNGMVTVNMGKPKFMPSEIPFVPESGEGDDACIYGVHLESGIQPVSCV
                     NMGNPHAVIVVDDVECAPVRETGSLIEPHRQFPERVNVGFMQVVGRTAIRLRVFERGV
                     GETQACGTGACAAVVAGIRLGLLDEGKTVEVILPGGTLYIEWACGGDVMMTGPAETVF
                     EGELAYS"
     gene            2868..3164
                     /locus_tag="WGQ68_001143"
     CDS             2868..3164
                     /locus_tag="WGQ68_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714945.1"
                     /translation="MFRILLLLFPCAILAACTRNIPANPAVSNCAHLQNRTILYFDAH
                     GKPADGAVKGGFYKIIEIQGANCILIQDFNSTGNALGGSYVPDCRKSSLFPKNI"
     gene            3178..3846
                     /locus_tag="WGQ68_001144"
     CDS             3178..3846
                     /locus_tag="WGQ68_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0714946.1"
                     /translation="MNHDITFLTLFLLGFFGGTHCIGMCGGLSSAFALQLPPHINRFW
                     LILLLNTGRVSSYTAIGLILGLIGQVGVSLDQTRVLQNILYTAANLLLLFLGLYLSGI
                     SSLAAKIEKIGKPIWRNLNPILNRLLPIKSIPACLAVGILWGWLPCGLVYSASLYALG
                     SGSAATGGLYMLAFALGTLPNLLAIGIFSLQLKKIMQNRYIRLCTGLSVSLWALWKLA
                     VLWL"
     gene            complement(3919..6039)
                     /gene="pnp"
                     /locus_tag="WGQ68_001145"
     CDS             complement(3919..6039)
                     /gene="pnp"
                     /locus_tag="WGQ68_001145"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEZ0714947.1"
                     /translation="MFDKHVKTFQYGNHTVTLETGEIARQAAAAVKVSMGDTVVLVAV
                     TTNKEVKEGQDFFPLTVDYLERTYAAGKIPGGFFKREGKQSEKEILTSRLIDRPIRPL
                     FPEGFYHDIQIVAMVVSVDPEIDSDIPAMLGASAALVLSGVPFAGPIGAARVGYINGV
                     YVLNPTKAELAKSQLDLVVAGTSKAVLMVESEAKILPEDVMLGAVVYGHDQMQAAINA
                     INEFADEVNPEVWDWKAPETNEELVAKVRGIAGETIKEAFKIRQKQARSAKLDEAWNA
                     VKEALITEETDTLAANEIKGIFKHLEADVVRSQILDGQPRIDGRDTRTVRPLNIQTGV
                     LPRTHGSALFTRGETQALAVATLGTSRDEQIIDALSGEYTDRFMLHYNFPPYSTGEVG
                     RVGAPKRREIGHGRLAKRALLAVLPKPEDFSYTMRVVSEITESNGSSSMASVCGGCLS
                     LLSAGVPLKAHVAGIAMGLILEGNKFAVLTDILGDEDHLGDMDFKVAGTTEGVTALQM
                     DIKIQGITKEIMQIALAQAKEARLHILDQMKAAVAGPQELSAHAPRLFTMKINQDKIR
                     EVIGKGGETIRSITAETGTEINIAEDGTITIAATTQEAGDAAKKRIEQITAEVEVGKV
                     YEGTVVKILDNNVGAIVSVMPGKDGLVHISQIAHERVRNVGDYLQVGQVVNVKALEVD
                     DRGRVRLSIKALLDAPAREENAAE"
     gene            complement(6276..7139)
                     /locus_tag="WGQ68_001146"
     CDS             complement(6276..7139)
                     /locus_tag="WGQ68_001146"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEZ0714948.1"
                     /translation="MSEIGLVKIYSGKVRDLYEIDDKRMLMVASDRLSAFDVILDDPI
                     PSKGEILTQISNFWFKKLAHIMPNHFTGQTVYDVLPENEAKVLEKRAVVAKKLTPVKV
                     EAIVRGYLAGSGWKDYQKTGSVCGIRLPEGMQEAQQLPEVIFTPSTKAAVGDHDENIS
                     FEECGRIIGKELAEEVRAKAVRLYTEAAEYAKSRGIIICDTKFEFGLDTNGTLTLMDE
                     VLTPDSSRFWPADQYKVGTNPPSFDKQFVRDWLEQSGWNKKAPAPKVPADVIQKTVEK
                     YREALTLLTQD"
     gene            7337..8212
                     /gene="rfbD"
                     /locus_tag="WGQ68_001147"
     CDS             7337..8212
                     /gene="rfbD"
                     /locus_tag="WGQ68_001147"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEZ0714949.1"
                     /translation="MRILLTGSKSQLARCLRDRLPEDWETIATDSASLDITDADAVCN
                     MVKSFQPDAIVNTAAYTAVDKAEGDAAAAFAVNASAVYNLALAAHRAHARFIHISTDY
                     IFDGKGKRPYQESDFTNPSNVYGQSKTAGELLALSANPDSLILRTSWLFSEYGDNFVR
                     TMLNLARERSPLSAVHNQIGCPTYAGDLSAAIIRLLQQSNPVRGIYHYVGGKSVSWYE
                     FAQHIFQAASQQQTSFPVPELTAVSDKEYPTAAPRPAYSILDCRKIENDFSIKPSDWQ
                     KALAQVVSKLLSSGY"
     gene            complement(8951..9184)
                     /locus_tag="WGQ68_001148"
     CDS             complement(8951..9184)
                     /locus_tag="WGQ68_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HEZ0714950.1"
                     /translation="MKLTLMFREYCSLCHKMRDALKPFQNEYGFGLEVVDVDENPVLE
                     EKYNELVPVLLAGDEEICHWFLDEDRLKQFLER"
     gene            complement(9350..9550)
                     /locus_tag="WGQ68_001149"
     CDS             complement(9350..9550)
                     /locus_tag="WGQ68_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HEZ0714951.1"
                     /translation="MFVCICNAVTDHQIKETIAAGATTMGDLQSQLGVASCCGCCGEL
                     AASFLTAHNAQPTVTAGINVQA"
     gene            complement(9722..10597)
                     /gene="xerD"
                     /locus_tag="WGQ68_001150"
     CDS             complement(9722..10597)
                     /gene="xerD"
                     /locus_tag="WGQ68_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEZ0714952.1"
                     /translation="MEEGLIDRLLETLWLDRRLSQNTLNGYRRDLEKIARRLSQSGRM
                     LKDADEADLAAAVYVDGEQRSSQARALSACKRLYIWMEREGIRTDNPTRLLKPPKIDK
                     NIPTLITEQQISRLLATPDTDTPHGLRDKALLELMYATGLRVSEAVGLNFGSVDLDRG
                     CITALGKGDKQRMIPMGQESAYWVERYYTEARPLLLKGRNCDALFVSQKKTGISRQLA
                     WMIVKEYASQAGIGHISPHSLRHAFATHLVRHGLDLRVVQDMLGHADLNTTQIYTHVA
                     NVWLQGVVKEHHSRN"
     gene            complement(10649..11089)
                     /locus_tag="WGQ68_001151"
     CDS             complement(10649..11089)
                     /locus_tag="WGQ68_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEZ0714953.1"
                     /translation="MDVKYEFTLPSSSGADFHSAEHLPLVVYFYPKDSTPGCTTEGLD
                     FNARLEQFEALGYTVVGISRDGVKAHQNFCAKQGFRFELLSDKDETVCRLFDVIKLKK
                     LYGKESLGIERSTFVLNKDGEIAHEWRKVKVAGHAQEVLETLSR"
     gene            11410..12348
                     /locus_tag="WGQ68_001152"
     CDS             11410..12348
                     /locus_tag="WGQ68_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEZ0714954.1"
                     /translation="MSIHTLKRLPSSLLLGLCLSLPSAHLFADNDILGQFLEQNMLTS
                     SDPIEIFAESTIHPTNTQAITGGLILSSQSALVVNNKTGQILYQKNADRIMPIASISK
                     LMSAMVVLDANLDMNETVTITPDEIDRIKGTGSRLAIGTALTRKKLLHLSLMSSENRA
                     THALGRTYPGGMGAFVAAMNRKAQSLGMYGSRFYEPTGLNFQNVSTAKDLSLMVNAAA
                     QYPQIRTNSTSNYASVQTKNGQQNYKNSNALVREGMWNIELQKTGYIREAGRSMVVKA
                     NIQNQPVTIVLLNSPTSATRVNDARKIESWMLQQRS"
     gene            12477..12770
                     /gene="hfq"
                     /locus_tag="WGQ68_001153"
     CDS             12477..12770
                     /gene="hfq"
                     /locus_tag="WGQ68_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HEZ0714955.1"
                     /translation="MTVKGQMLQDPFLNALRKEHVPVSIYLVNGIKLQGQVESFDQYV
                     VLLRNTSVTQMVYKHAISTIVPARSVNLQHENRPQAAPTSTLVQVETVQQPAE"
     gene            12855..13421
                     /locus_tag="WGQ68_001154"
     CDS             12855..13421
                     /locus_tag="WGQ68_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0714956.1"
                     /translation="MLLQNILPFAHCLLRKALPEGGNALDGTAGNGHDTLFLAQTAGI
                     RGKVWAFDIQPQALSRTRCRLKEARYENVQLILDGHENLKQYVSEPLNAAIFNFGWLP
                     GGDKSLTTHTETSIPALCATLSLLKEGGMLIAVLYPGHENGKQEAEAIEQWAKNLPQE
                     QFAVLRYGFTNRKNNPPYLLAFEKLRQK"
     gene            13475..13849
                     /locus_tag="WGQ68_001155"
     CDS             13475..13849
                     /locus_tag="WGQ68_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HEZ0714957.1"
                     /translation="MQYLIVKYSHQIFVTITILVFNIRVFLLWKNPEKPLVGFWKALP
                     HLNDTMLLFTGLWLMKITHFSPFNAPWLGTKILLLLAYIALGMMMMRARPRSTKFYTV
                     YLLAMCCVACIVYLAKTKVLPF"
     gene            13859..14353
                     /gene="folK"
                     /locus_tag="WGQ68_001156"
     CDS             13859..14353
                     /gene="folK"
                     /locus_tag="WGQ68_001156"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEZ0714958.1"
                     /translation="MNNRHFAVIALGSNLENPAQQVRAALDTLSSHPDIRLEQASSLY
                     MTAPVGYDNQPDFVNAVCTVSTTLDGIALLAELNRIEADFGRERSFRNAPRTLDLDII
                     DFDGISSDDPRLTLPHPCAHERSFVIRPLAEILPDFVLGKHGKVAELSKRLGNQGIRL
                     LPDK"
     gene            complement(14480..14959)
                     /locus_tag="WGQ68_001157"
     CDS             complement(14480..14959)
                     /locus_tag="WGQ68_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HEZ0714959.1"
                     /translation="MPESIFKQISLDILKLHRDSVYSLLATSGCNCQVHEAAYVNIDG
                     KYYISLSCEPEVGEVKTGILLIEDESRNLRLSWVGSARELDRKDSVYKRALSALSRKL
                     GRCKDRLHTAVQPFLLELVPEKGRFSVGDEEVWISRNDLVRALYPVGYSMRQAMLQI"
     gene            complement(15179..15916)
                     /gene="ubiE"
                     /locus_tag="WGQ68_001158"
     CDS             complement(15179..15916)
                     /gene="ubiE"
                     /locus_tag="WGQ68_001158"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HEZ0714960.1"
                     /translation="MGGQKTHFGFSTVNEDEKAGKVAEVFHSVAKNYDIMNDVMSAGL
                     HRVWKHFTINTAHLKKGDKVLDIAGGTGDLSRGWAKRVGKEGEVWLTDINSSMLTVGR
                     DRLLNEGMILPVSLADAEKLPFPDNYFNLVSVAFGLRNMTHKDTALKEMCRVLKPGGT
                     LLVLEFSKIYKPLEGAYDFYSFKLLPVMGKLIAKDAESYQYLAESIRMHPDQETLKQM
                     MLDAGFDSVDYHNMSAGIVALHKGVKF"
     gene            complement(15959..16327)
                     /locus_tag="WGQ68_001159"
     CDS             complement(15959..16327)
                     /locus_tag="WGQ68_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HEZ0714961.1"
                     /translation="MNNIPVEIRLLKNRTVLVLTYGDEPKNLPAEFLRVYSPSAEVRG
                     HGVGQDVLQTGKADVQITDLQPVGQYALKISFSDGHDSGLYDWAYLHRLAYGYDAMWQ
                     EYLDKLAAAGASRFEKRSDC"
     gene            16447..18066
                     /gene="mnmC"
                     /locus_tag="WGQ68_001160"
     CDS             16447..18066
                     /gene="mnmC"
                     /locus_tag="WGQ68_001160"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HEZ0714962.1"
                     /translation="MDNLVWDGVPDIRTLDQTIRKHAHPLNLIVCLPDNQIPDFQTAQ
                     DASDAECRLKHRLDQATQCLQFDSINLIEHILPDVRFWLVPPSRTRRLHEHFHHISWQ
                     TEAIPQTESKPDKPWFALPQTSERQKPEHILVIGAGISGAATAHALASYGISVTVLEA
                     RKAAQAASGNRQGLLYAKISPHNTEQTELLLTGYGYTKRLLGHILPESETWGGNGIIH
                     LNYSRTEQQRNHELGLQKHHNHLYRSITQAEAEKIAGIPLSVPYDHPSCGLYWQHGVW
                     LNPPAFVRALLNHPLIGLHEDTPLTDISHDGEKWIASTPNGIFSATHIIYCTGAHSSC
                     LPETNLAALPLRQIRGQTGLTPSTPFSEQLRCAVSGESYISPSWHGLHCYGASFIPNS
                     SHTGWNEAEEASNRQALAQLSPALAESLFAANPIPQKHQGHAAMRCDSPDHLPLVGAL
                     GDIAAMQQTYAKLALDKNYRIDAPCPYLPNAYANTAHGTRGLATAPICAAAVAAEILG
                     LPHPLSKRLRHALHPNRTIIRAIVRRKDLTP"
     gene            complement(18063..18212)
                     /locus_tag="WGQ68_001161"
     CDS             complement(18063..18212)
                     /locus_tag="WGQ68_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714963.1"
                     /translation="MDSKGNLLGIPYSLFWEENVMGVFDILPSGLVCLFEYDFLWSGR
                     HAAKA"
BASE COUNT         4717 a         5459 c         3934 g         4298 t
ORIGIN      
        1 aaaatatttc cctcaagcga tgcggcgaca ccaacacctg attaaaacac aaccggcgtt
       61 ttcggcatat atgcaaaaac gcatttccca gcacggcgtt acaccgatac cgaaaaatgc
      121 gtctgaatgt ttcgacaatc cgattaaagc attatgccaa atctgcaaaa agtggcgtag
      181 agagatagcg ttcgccataa gaaggcagca gcacgactat cagcttgcct tcgttttcag
      241 gctgtttggc aagctgcaac gcactccaaa ccgccgcacc ggaagaaata cccaccaaaa
      301 tgccttcttt ttccgctatt gcgcgggcgg tttcaaaagc cgcttcgttc ggcactttgg
      361 taatgctgtc gtaaattttg gtattcaaaa cggtcggaat aaagcctgcg ccgatgcctt
      421 gaatcgggtg cgggcctttt tcgccgccgc tcaatacggg tgaagcttca ggctcgacgg
      481 caaccacttt aacttcgggt ttgtattttt tcaacacttc gcccacgccg gtaatcgtac
      541 cgcccgtgcc gacgccggca acgaagacat cgactttgcc gtccgtatcc cgccaaattt
      601 cctcggcggt tgttttgcgg tggacttcgg ggtttgcctc attgtcgaac tggcgcggca
      661 taaaataagt gtcgggatgc gcgtccacca aggatttcgc tttggcaatc gcgcccgcca
      721 taccttcggc ggcgggggtc agaatcagct ccgcaccaaa cgcgcgcaac agcattttgc
      781 gctccttact catgctttcc ggcatggtaa tcgccagctt gtagccgcgt gcggcacata
      841 ccattgccaa accgacaccc gtattgccgc tggttgcttc gacaatgacg gtgtttttgt
      901 tgattttgcc cgctttttcg gcaccctcaa tcattgcttc ggcaatgcgg tctttgacgc
      961 tgctgcccgg attgaaaaat tccagtttca cggcaacctc tgccttcaaa ccttcggtca
     1021 gacggttcag tttgaccaaa ggcgtgttgc cgattagctc ggtgatgctg tttgcaattt
     1081 tcatcttctg ctcctgaata ataaggtttc gacagtaagg cgcaatcccc ataggcatcc
     1141 tgtccgattg caggggattt gcgccgaaac ataaacaaat ttcggatatg gctttatcct
     1201 aaacctatgc cgcctaaaaa accaataccc aaaatttctt tctttataac tttttgttcc
     1261 aatacggccg caccgtatta tcttccaatt tctgaaagca gcagcaccag taccgagacc
     1321 tgcatcaacg ccgccgccaa cacgccgagg cgcagctgcg aaccgccgaa cgttgacggc
     1381 ggctgccggt ctataccgca gcggtggaca taagcaagca ccatcagcac aaactgcaac
     1441 acaaaccaat aaaccggctt cagcgcataa agtttgagca gtgccgcaaa cacaaacact
     1501 tgaaccgttc ccgaaaagca ataatgcacc aacgcacata ttccgacaaa agcaagcgtc
     1561 atcgatacat tactgacggc aaaaagcccg agcagcggat gttcggggtc tgcctgccat
     1621 ccgtttccat ctgttttccg gttccaatgc ccgatgaaaa aaaatatgct gcccaaagtc
     1681 aggtaaaaat gggggatgaa caaaggcgcg gcgcgggtca ttccccatat gccgggcatc
     1741 agtttcgccc ccagaaccga tatgccgagc cacagcgcaa tactcgccca aaaccattgc
     1801 aacattcctg cgcggaatat cagaaacaga aaaaccgccg catacagtaa ggcagacaaa
     1861 aaatccaaat gcagcaaaat catgaatacg ccaactcgcc ttcaaacacg gtttccgcag
     1921 ggccggtcat catcacatcg ccgccgcagg cccattcgat atataaagtt ccgcccggca
     1981 aaatcacctc taccgttttc ccttcatcca acaaccccag acggataccc gccaccacag
     2041 ccgcacacgc gcccgtaccg cacgcttggg tttcgcccac gccgcgctcg aacacgcgca
     2101 aacgaatcgc ggttcggccg acaacctgca taaagccgac attgacgcgt tcgggaaact
     2161 gcctgtgcgg ttcgataagc gaaccggttt cgcgcaccgg cgcgcattcc acgtcatcga
     2221 ccacaatcac cgcatgggga ttgcccatat tgacgcagct gacaggctga atgccggatt
     2281 cgagatgcac cccgtaaata caggcatcat ccccctcgcc cgattcgggg acaaacggta
     2341 tttcagacgg cataaacttc ggtttgccca tattgaccgt aaccataccg ttatcggaca
     2401 atttcggaaa aataacgcca tttgccgttt caacacaaat ttctttctta tcggtcaaac
     2461 ccttgtctgc aacaaaacgg gcaaaacaac gcgccccgtt gccgcactgc ccgacctcgc
     2521 tgccgtcggc attgaaaata cggtaacgga aatccacgcc ttcggtttcc gaacgcccga
     2581 ccaccaaaag ctggtcgaag cccacgcctc ggaagcggtc cgcccatgcc gcaatcggcg
     2641 cgtcctcggg ggtaaaatcc tgactgaccg cgtcaatcac cataaaatcg ttgcccaaac
     2701 cgtgcatttt ggtaaatttc aacgtgttca tattgttccg aaatcccgta tcgtctgttg
     2761 cttccgaatc cgaaatcccc cgcaccgaag ggaaatgctt ccatctcccg ccgcctgagg
     2821 attaaaatca gccattatac tgtttgttac cccttacaag aaacaccatg ttccgcatat
     2881 tactgttgct tttcccttgt gcaatccttg ccgcctgcac ccgaaacata ccggcaaacc
     2941 cggccgtttc aaattgcgcg catcttcaaa accgcaccat cctgtatttt gacgcacacg
     3001 gcaaacccgc cgacggcgca gtaaagggcg gattttacaa aattatcgaa atacagggtg
     3061 caaactgcat ccttattcaa gactttaaca gcacgggcaa tgccctcggc ggatcctatg
     3121 tgccggactg ccgcaaatca tcccttttcc cgaaaaatat ttgacttcaa actgcctatg
     3181 aaccacgaca tcactttcct caccctgttc ctactcggtt tcttcggcgg aacgcactgc
     3241 atcggtatgt gcggcggatt aagcagcgcg tttgcgctcc aactcccccc gcatatcaac
     3301 cgcttttggc tgatcctgct gcttaacaca ggacgggtaa gcagctatac ggcaatcggc
     3361 ctgatactcg gattaatcgg acaggtcggc gtttcactcg accaaacccg cgtcctgcag
     3421 aatattttat acacagccgc caacctcctg ctgctctttt taggcttata cttgagcggt
     3481 atttcttcct tggcggcaaa aatcgagaaa atcggcaaac cgatatggcg gaacctgaac
     3541 ccgatactca accggctgtt acccataaaa tccatacccg cctgccttgc ggtcggaata
     3601 ttatggggct ggctgccgtg cggactggtt tacagcgcgt cgctttacgc gctgggaagc
     3661 ggtagtgcgg caacgggcgg gttatatatg cttgcctttg cactgggtac gctgcccaat
     3721 cttttggcaa tcggcatttt ttccctgcaa ctgaaaaaaa tcatgcaaaa ccgatatatc
     3781 cgcctgtgta cgggattatc cgtatcatta tgggcattat ggaaacttgc cgtcctgtgg
     3841 ctgtaagccc cattcattcc cgatacccgt aaaaaatacc gtctgaaacc tgttcagacg
     3901 gcactttcac cctaagcgtt actcggcggc attttcctca cgggcaggcg cgtccagcag
     3961 ggctttgatg gacaggcgca cacggcctct gtcatccact tccaatgctt tcacgttcac
     4021 cacctgaccg acttgcaggt agtcaccgac attgcgtacg cgctcgtggg cgatttggct
     4081 gatgtgtacc aaaccgtctt tgcccggcat cacgctgaca atcgcgccga cgttgttgtc
     4141 gaggattttc accacagtgc cttcatacac tttgcccact tccacttcgg cagtaatctg
     4201 ctcgatacgt tttttcgccg catcgccggc ttcttgagtg gtcgcagcaa tggtaatcgt
     4261 accgtcttcg gcaatattga tttccgtacc ggtttcagcg gtaatcgaac ggatggtttc
     4321 gccgccctta ccgataactt cgcggatttt gtcttggttg attttcatcg taaacaaacg
     4381 cggcgcgtgt gcggacaact cttgcgggcc cgcaacggcg gctttcatct gatccaagat
     4441 gtgcagacgc gcttctttgg cctgtgccaa agcgatttgc ataatttctt tggtaatgcc
     4501 ttggattttg atgtccattt gcagcgcggt aacgccttcg gtcgtaccgg ccactttaaa
     4561 gtccatatcg cccaagtggt cttcgtcgcc caaaatgtcg gtcaggacgg caaatttgtt
     4621 gccttccaga atcagaccca tcgcgatacc ggcaacgtgt gctttcaaag gcacgccggc
     4681 agacagcagg ctcaggcagc cgccgcagac ggaagccata gaggaagagc cgttggattc
     4741 ggtaatttcg gagaccacgc gcatggtgta gctgaaatct tcaggtttcg gcaatacggc
     4801 caacaatgca cgtttagcca aacggccgtg accgatttca cggcgtttcg gcgcacctac
     4861 gcgacccact tctccggtag agtacggcgg aaagttgtag tgcagcatga agcggtcggt
     4921 gtattcgccg gacagcgcgt caatgatttg ctcgtcgcgc gaagtaccca aagttgcaac
     4981 cgccaaagct tgggtttcgc cacgggtaaa caatgcggaa ccgtgtgtac gcggcaatac
     5041 gccggtttgg atgttcaaag gacggacggt gcgggtgtcg cggccgtcga tgcgcggttg
     5101 accgtccaaa atttggctgc ggacaacatc ggcttccaag tgtttgaaaa tacctttgat
     5161 ttcgttggcg gccaaagtat cggtttcttc ggtaatcaag gcttctttta ccgcattcca
     5221 agcctcgtcc aatttggcgg aacgcgcttg tttttgacgg attttgaacg cttctttaat
     5281 ggtttcgccg gcaatcccgc ggactttggc aactaattct tcattggttt caggtgcttt
     5341 ccaatcccaa acttccggat tgacttcgtc ggcaaattca ttgatggcgt taatcgccgc
     5401 ctgcatttga tcgtggccgt aaaccaccgc gcccaacatc acgtcttcgg gcaggatttt
     5461 ggcttcggat tccaccatca acacggcttt ggacgtaccg gcgaccacca agtccaattg
     5521 cgatttagcc aattcggctt tagtcgggtt caaaacgtac acgccgttta tataacccac
     5581 gcgtgccgca ccaatcggac cggcaaacgg tacgccgctc aacaccagcg cggcagacgc
     5641 gcccaacatt gcaggaatgt cggaatcgat ttcgggatcg acggacacga ccatcgctac
     5701 gatttggatg tcgtggtaga aaccttcggg gaacagcgga cgaatcgggc ggtcgatcag
     5761 acggctggtc aggatttctt tttcgctttg tttgccttcg cgtttgaaga aaccgccggg
     5821 gattttacct gcggcgtaag tgcgttccaa ataatcgacg gtcaggggga agaagtcttg
     5881 accttctttc acttctttgt tggtggtaac ggcaaccaaa acaacggtgt cgcccataga
     5941 gactttaacg gcagcggcgg cttggcgggc aatttcgccg gtttccaaag taacggtgtg
     6001 gttgccgtat tggaaagttt taacgtgttt gtcgaacatt attgttcctt tcaaaatacc
     6061 gcactgctaa aacactaata atgcacacta aaatccgaat gtgcatagtt agggtttcag
     6121 accgtgcggc aggttgtaaa caagctttca gacgacattt cggacgctga aagcaattgc
     6181 ggattataaa ggcaaccatc cttaaaatcc agtattgata caataaaaag gccgtctgaa
     6241 acatatttct ttcagacggc cttcaaactt aaaaatcaat cctgagtcag caaagtcaat
     6301 gcttcccgat acttctcgac agttttctga atcacatcgg caggcacttt cggcgcaggg
     6361 gcttttttgt tccaaccgct ttgttccagc cagtcgcgga caaattgttt gtcaaaagac
     6421 ggcggatttg tgccgacttt atattggtcg gcaggccaga agcggctcga atcgggggtc
     6481 aatacctcat ccatcagcgt cagcgtaccg tttgtatcca aaccgaattc aaatttggta
     6541 tcgcaaataa taataccgcg cgatttggca tattccgccg cttcggtgta aagccgaacc
     6601 gccttggcgc gcacttcttc cgccaattct ttgccgataa tgcgtccgca ttcttcaaag
     6661 ctgatgtttt catcgtgatc gccgactgcg gctttggtcg atggcgtaaa aatcacttca
     6721 ggcagttgtt gcgcttcctg cataccttcg ggcagccgaa taccgcaaac cgagccggtt
     6781 ttttgataat ctttccaacc gctgcctgcc agataaccgc gcacaatcgc ctctactttc
     6841 accggagtga gctttttagc cacgacggcg cgtttttcta aaactttggc ttcgttttca
     6901 ggcaaaacat cgtaaaccgt ttgaccggta aagtggttgg gcataatatg cgccagtttt
     6961 ttaaaccaaa aattggaaat ctgcgtcaga atctcccctt tgctcggaat cgggtcgtcc
     7021 aaaatcacat caaacgcgga caggcggtcg gaagcgacca tcagcatacg tttatcgtcg
     7081 atttcatata aatcgcgcac ttttccagaa tagatcttta ccaaaccaat ctcactcatt
     7141 tcgcccccct gaaaatatct tgaaaatacc gacccgacac ccgacaggtt tgaatcgcaa
     7201 accgatattc tagccgaagt cggcgcaaaa caatacccat ggcacaaaaa gccaacccgt
     7261 caaccgtcgg caaagttttg gcactataat accgacagca agtcctacaa tacactttta
     7321 ccaaaggaaa tacctcatga gaatcctatt gacaggctcg aaaagccaac tggcacgctg
     7381 cctgcgcgac cgtcttccgg aagactggga aaccattgcg acggattccg catccctaga
     7441 cattaccgat gccgatgccg tctgcaacat ggtcaaaagt ttccaacccg acgccattgt
     7501 caacacggct gcctatactg ccgtcgacaa agcggaaggc gatgcggcag cggcatttgc
     7561 cgtcaatgct tccgccgttt acaaccttgc cttggcagca catcgcgccc atgcccgatt
     7621 catccacatc tcaaccgact atatctttga cggaaaaggg aaaagaccct accaggaaag
     7681 cgactttacc aacccttcca atgtatacgg acaatccaaa accgccggcg aactgctcgc
     7741 actgtctgcc aatcccgaca gccttatcct gcggacttct tggctgttta gcgaatacgg
     7801 ggacaacttt gtccgcacca tgctgaacct tgcgcgggaa cgttccccgc tgtccgccgt
     7861 ccacaaccaa atcggctgcc cgacctatgc cggcgacttg tccgccgcca tcatccgcct
     7921 gttgcagcag tccaatcccg ttcgcggcat ttaccactac gtcggcggca aatccgtatc
     7981 ctggtacgaa tttgcccaac atattttcca agcggcatcg caacagcaga catccttccc
     8041 cgttcccgaa ttgactgccg tttcagacaa ggaatatccg accgccgccc ccaggcccgc
     8101 atacagcatt ttggactgcc gcaaaatcga aaacgacttc agcatcaaac cgtcagactg
     8161 gcaaaaagcc cttgcacagg tcgtttccaa gctgctctct agtgggtact aggtataatg
     8221 caggcatccg ataaagcaaa gcccccgaag ctgcaacttc gggggcttgt cggatagaaa
     8281 ggaggcttga agaaatggcg aaagtcattt tattcctgtt tcttttgtta gtttgctctc
     8341 cagcctacta atgaaaaact gaaataacaa ggggtggcaa attaccgcgc ccctttcgcc
     8401 aacctttctt cgagccattt tactacaatg gctttaaaaa tcaaggattg cctgatgctg
     8461 cccgcccctc cgtttccgcc gccaagcacc gccttggcgg ttttcttata tagtggatta
     8521 acaaaaacca gtacggcgtt gcctcgcctt gccgtactat ttgtactgtc tgcggcttcg
     8581 tcgccttgtc ctgatttttg ttaatccact ataaaatcta ccgattacac aaacacatat
     8641 catctttaca caaacacata tcatctttac acaatcatgc ttccatcaac agtaaaacat
     8701 gatatgattg ccaacaataa catctcacaa taaattttct aatttttatt gaaaaaatca
     8761 ataaataaga attctcccaa ccgacaaata aaataaagaa agggttaata tgcaacatcg
     8821 aagaagacta gcaatttacc aagcatccaa acgtgcttcc tttaccggca ggtcatccgc
     8881 cccgcaaaaa cgtaaagaac gttaatttga aaaaaatgcc gtctgaagtc ctgcttcaga
     8941 cggcattttt ttaccgttcg agaaactgtt tcaacctgtc ctcatccaaa aaccagtgac
     9001 agatttcctc atctcccgcc aacaaaacgg gaaccagctc attgtatttt tcttccaaaa
     9061 caggattttc atccacatcg accacttcca gcccgaaccc gtattcattt tgaaaaggtt
     9121 tgagtgcatc gcgcattttg tggcacaagc tgcaatattc acgaaacatc aaggtcaatt
     9181 tcatccgcgt ttccttatct gtcaatttgc acacgccaaa gccttagacg cagcagaatc
     9241 atggtctatt tgggaaaaaa caatgttttc aaggaagatg atactcaagt cctgccaaaa
     9301 acagcaaaaa tgccgtctga acagttcaga cggcatttcc aaaaccgttt tacgcttgaa
     9361 cattgatacc cgccgtaacc gtcggttgcg cattgtgcgc cgtcagaaac gaagcggcaa
     9421 gctccccaca gcagccgcag cagctcgcta cgcccaattg cgactgcaaa tcgcccattg
     9481 tggtcgcgcc ggcggcgatg gtttccttga tttgatggtc ggtaacggca ttgcagatgc
     9541 agacaaacat tttgtgctcc gtgtgttctc aaactatcgt accgatagcg gcttttatta
     9601 tcgtatgcaa atataaataa aaacggttcg cattgcaagg tcggcataca cggtttatct
     9661 tggtaatttt ttatcaagtt ttcatttccg attgatttct tattaaaccg aagcagaagc
     9721 ctcagtttcg ggaatggtgt tccttcacta caccctgcaa ccatacgttg gcaacatggg
     9781 tataaatctg cgtcgtattc aaatcggcat gtcccaacat atcctgaacc acgcgcaaat
     9841 ccaagccgtg ccgcaccaga tgcgtggcaa aggcgtggcg caggctgtgc gggctgatgt
     9901 gcccgatgcc tgcctgactt gcatattctt tgacaatcat ccatgccaac tgacgggaaa
     9961 tgcccgtctt tttctgactg acaaacaatg cgtcgcaatt cctgcctttc agcagaagtg
    10021 ggcgtgcctc cgtataatag cgttccaccc aatacgccga ctcctgcccc atcgggatca
    10081 tcctctgctt gtcgcccttg cccagcgcgg taatacagcc cctgtccaaa tccacactgc
    10141 cgaaattcag cccgaccgcc tcgctgacgc gcaagccggt cgcgtacatc aattcgagca
    10201 gtgccttgtc ccgcaaaccg tgtggcgtat cagtatccgg agtggcaagc agccgggaaa
    10261 tctgctgttc tgtaatcagt gtcggaatat tcttgtcgat tttgggcggt ttcagcaaac
    10321 gggtgggatt gtccgtcctt atgccttcac gctccatcca tatatacagg cgtttgcacg
    10381 ccgataatgc gcgcgcctgc gaactccgtt gctctccgtc aacataaacc gccgccgcca
    10441 aatccgcttc gtccgcatcc ttcagcattc tgcccgattg ggacaggcgg cgggcgattt
    10501 tttccaaatc gcgccggtaa ccgtttaaag tattctgact gagccgcctg tccaaccaca
    10561 gcgtttcaag cagcctgtcg atcaaacctt cttccatacc gttccaaaca aatgccgtct
    10621 gaatcttctt cagacggcat ggttcacatt atcgggaaag cgtttccaat acttcctgcg
    10681 cgtgacccgc cactttgact ttccgccatt catgggcgat ttctccatcc ttattcaaga
    10741 cgaacgtact gcgctcgata cctaacgact ctttcccgta cagtttcttc aatttgatga
    10801 catcaaacag gcggcacact gtttcatcct tgtcgctcaa cagctcgaac cggaaaccct
    10861 gcttggcgca aaaattctga tgcgccttta cgccgtcgcg ggaaataccg accacggtat
    10921 aacccaatgc ctcaaactgt tccaaacgcg cattgaaatc caagccttcc gtcgtacagc
    10981 ccggcgtact gtctttcgga taaaaataca cgaccaaagg cagatgttct gccgaatgaa
    11041 aatccgcacc gctgctcgaa ggcagggtaa attcatattt cacatccata gtcctactcc
    11101 cgatattccc attattcaaa acgacacgca gacgcaccgc cgcaattgcc aaaccaaccc
    11161 cgattctacc gccccaaagg acagggattc aaccgccgga aacatccaaa ccgacacacg
    11221 acggcatgaa aaatatccat gtcaaaccac aaaatatgtt ccgatttaaa aacagaatgt
    11281 tataaaaccc aatccccaaa acacaacaag accgcccgcc tacgggcagt ctcctgtcag
    11341 acgacatact ttacagatgg ctgttttttc aacaaaataa cgccaatact caaaaatatg
    11401 gaatcaaaaa tgtccatcca tactctgaaa cgcctgccct catcgctgct gctcggtctc
    11461 tgcctttccc tgccgtcagc ccaccttttt gccgacaacg acattttagg gcaattttta
    11521 gaacagaaca tgcttacctc ctccgatccg atagaaatat tcgccgaaag cacgatacac
    11581 cccaccaaca cccaagccat tacaggcggt ctgattctct cctcacagtc tgccctggtc
    11641 gtcaacaaca aaaccggaca gatactgtat cagaaaaacg ccgacaggat tatgcccatc
    11701 gcctccattt ccaaactgat gagcgcgatg gtcgttttgg atgcaaactt ggacatgaac
    11761 gaaaccgtta ccattacgcc cgacgaaatc gaccgcatca aagggaccgg cagccgtctt
    11821 gccataggta cggcacttac acgcaaaaaa ctgctgcacc tgagcctgat gagcagcgaa
    11881 aaccgcgcca cccatgcatt gggcagaacc taccccggcg gcatgggcgc atttgtcgcc
    11941 gccatgaacc gcaaagccca aagcctcggt atgtacggca gccgctttta cgaaccgacc
    12001 ggactcaact tccaaaacgt ttctaccgcc aaagacctga gccttatggt caacgccgcc
    12061 gcccaatatc cgcaaatccg caccaactcg acttccaact acgcctcggt acagaccaaa
    12121 aacgggcagc agaactacaa aaactccaat gccctggtca gagaaggcat gtggaacatc
    12181 gaattgcaga aaaccggcta catacgcgaa gcaggcaggt ctatggttgt caaagccaac
    12241 attcaaaacc aacccgttac catcgtattg ctgaactcgc ccacatccgc cacacgcgtc
    12301 aacgacgccc gcaaaatcga atcgtggatg ctgcagcaac gctcctgaca tacaaatgcc
    12361 cggcggaaaa ccgcccaaac acaaaaaaaa ttggtaattt ttcctattca aggctacaat
    12421 tcgacacgca cagggcgtct gcccgatttc acaatcataa taacggagtt aaaaatatga
    12481 cagttaaagg acaaatgttg caagatccct tcctgaacgc cctgcgtaaa gagcacgttc
    12541 cggtttcgat ttacttagtt aacggtatca aattacaagg tcaggttgag tctttcgatc
    12601 aatacgttgt tctcctgaga aacacttccg tcacccaaat ggtttacaaa cacgccattt
    12661 ccaccatcgt accggcacgc tccgtcaacc tacaacatga aaacagaccc caagccgcac
    12721 cgacttcgac cctcgtccaa gtggaaaccg tccagcagcc tgccgaataa tccgcacgaa
    12781 gcatgacgtg tcatatcttt caatacctta ccggacaacg gtaaggtatt tttattttca
    12841 gacagcattt aaaaatgtta ttgcaaaaca tccttccatt cgcccattgc cttttgcgga
    12901 aggcacttcc cgaaggtggc aatgctttgg acggcaccgc cggcaacgga cacgacaccc
    12961 ttttcctcgc acaaaccgca ggcatccggg ggaaagtgtg ggcattcgac atccagccgc
    13021 aagccctgag ccgcactcga tgccgtctga aagaagcgag atacgagaat gtgcaactca
    13081 tcttagacgg acacgaaaac ctgaaacaat atgtttcaga accactaaat gccgccattt
    13141 tcaactttgg ctggctgccc ggcggagata aaagccttac cacccatacg gaaaccagca
    13201 ttcccgccct ttgcgcaacc ctctccctgc tgaaagaagg cggtatgctt attgccgtcc
    13261 tctatccggg acacgaaaac ggcaaacagg aggcagaagc aatcgaacaa tgggcaaaaa
    13321 acctgcctca agaacagttt gccgttttgc gttacggctt taccaaccgg aaaaacaacc
    13381 cgccctatct gttggcattt gaaaagttac gtcaaaaata aatgtttgcg gtaaaataag
    13441 ccgattctaa cccttatttt gatacgagcc gaccatgcag tatctgattg tcaaatacag
    13501 ccatcaaatc ttcgttacca tcaccatttt ggtattcaac atccgtgttt tcctactttg
    13561 gaaaaatcca gaaaagccct tggtcggctt ttggaaggca ctgccccacc ttaacgacac
    13621 catgctgctg tttacgggat tgtggctgat gaaaattacc catttctccc cgttcaacgc
    13681 gccttggctc ggtacaaaaa tcctgcttct gctcgcctat atcgcattgg gtatgatgat
    13741 gatgcgcgcc cgtccgcgtt cgaccaagtt ctacaccgtt tacctgctcg ccatgtgttg
    13801 cgtcgcctgc atcgtttacc ttgccaaaac caaagtcctg cctttctgaa acaccgttat
    13861 gaacaacaga cattttgccg tcatcgccct gggcagtaat cttgaaaacc ctgcccaaca
    13921 ggtacgcgcc gcattggaca cgctgtcgtc ccatcctgac atccggcttg aacaggcttc
    13981 ctcactgtat atgaccgccc ccgtcggtta cgacaatcag cccgatttcg tcaatgccgt
    14041 ctgcaccgtt tccaccactc tggacggcat tgccctgctt gccgaactca accgtatcga
    14101 agccgatttc ggacgcgaac gcagcttccg caacgcgccg cgcacattgg atttggacat
    14161 tatcgacttt gacggcatct ccagcgacga cccccgactc accctgccgc atccgtgcgc
    14221 gcacgaacgc agtttcgtca tacgcccttt ggcagaaatc ctccctgatt ttgttttagg
    14281 caaacacgga aaggttgccg aattgtcaaa acggctgggc aatcaaggca tccgtctttt
    14341 accggataag taattccgca cgcggatgcc gtctgaatgc cggtttcaga cggcattttt
    14401 cctttgccgc caacacgcgt gcaaaaaaat cgccccttgg aaaaggggcg caaaaggaac
    14461 acaaaccact accaaaactt taaatctgca acattgcctg ccgcatactg tatccgacag
    14521 gatataaagc cctcactaaa tcgtttcgag aaatccaaac ttcttcatcg ccgacagaaa
    14581 atctgccttt ctccggcacc agctccaaca aaaacggttg aaccgccgta tgcagcctgt
    14641 ccttacaccg ccccagcttt ctggacaagg cggacagggc acgcttatag acactatcct
    14701 tgcggtcaag ttcccgcgca ctgccgaccc agctcaaacg aaggttgcgg ctttcatcct
    14761 caatcagcaa aatgcctgtt ttgacttccc ctacctctgg ctcgcatgaa agcgaaatat
    14821 aatatttgcc gtcgatgttg acatacgccg cctcatgcac ctgacagttg cagccggaag
    14881 tggcaagcag cgaataaaca gaatcccgat gcagtttcaa aatatcaagg gaaatctgtt
    14941 tgaaaatact ttccggcatt tttccctcct ttcgtctgct gcgttgttta ctggattgac
    15001 tgcattatac agacatctcc caatcttgca aatgataatt gttcctattt tttaaaaaat
    15061 ttataaatta ttgatagata tgtgaattaa ttttaagatt taaaatctct ttctcaaaaa
    15121 aacacaaaaa tgccgtctga aacgtgttca gacggcattg gctgcacagg cagtccgttt
    15181 aaaatttcac gcccttatgc agcgcgacga tgcccgcact catattgtga taatccacgc
    15241 tgtcaaagcc tgcatccagc atcatctgtt tcaaagtttc ctgatcgggg tgcatacgga
    15301 tggattcggc aagatattga taactctccg catctttggc aatcagtttg cccatgaccg
    15361 gcagcagctt gaacgaataa aaatcatacg cgccttcgag tggcttgtag attttggaaa
    15421 actccaatac cagcaatgtc ccgccgggtt tcaaaacacg gcacatctct ttcagcgcgg
    15481 tatccttgtg cgtcatgttc cgcaaaccga acgccacgga aaccaagttg aaataattgt
    15541 cagggaaagg cagtttttcc gcatcggcaa gcgacactgg caaaatcatg ccttcgttca
    15601 acagacggtc gcgcccgacg gtcagcatag aggaattaat atcggtcagc caaacctcgc
    15661 cttccttgcc gacccgtttc gcccaaccgc gcgacaaatc gcccgtaccg cccgcaatgt
    15721 ccaacacttt atcgcctttt ttcaggtgcg ccgtgttgat ggtgaaatgc ttccacaccc
    15781 tgtgcagccc tgccgacatc acatcgttca taatgtcgta gtttttggcg acggagtgga
    15841 acacttccgc cacttttccg gctttctcat cttcgttgac cgtgctgaac ccgaaatgcg
    15901 ttttctgtcc gcccatattc taaccacctc cctgatgccg tcctccggca tcccaaactt
    15961 aacaatctga tcgcttttca aaacgggaag cacccgccgc cgccaatttg tccaaatatt
    16021 cctgccacat cgcatcgtat ccgtatgcca gtctatgcag atacgcccaa tcgtaaagac
    16081 cgctgtcgtg cccgtccgaa aaactgattt tcagcgcgta ctgtccgaca ggctgcaaat
    16141 ccgtaatttg cacatccgcc ttgccggtct gcaaaacatc ctgtcccacg ccgtgtccgc
    16201 gcacttccgc actcggcgaa tagacgcgta aaaattcggc aggcagattt ttaggttcgt
    16261 ccccataagt caaaaccaac acggtacggt ttttcaacaa acggatttca acaggaatat
    16321 tattcaaaaa acacctcggt tcggcataca atatccggca ggcgcaggcg tgccttcaaa
    16381 cggcgcgtat tttaacagct ttatcccgat accgatattc aaacccacac tttaaacggc
    16441 attgatatgg acaatctcgt atgggacggc gttcccgaca tccgcacact cgaccaaacc
    16501 atccgcaaac acgcacaccc gctcaacctg attgtctgcc tccccgataa tcagattccc
    16561 gattttcaaa ccgcacaaga tgcttcggac gcggaatgcc gtctgaagca ccgtttggat
    16621 caagcaacgc agtgcctcca gttcgacagc atcaacctca tcgaacacat cctgcccgat
    16681 gtccgcttct ggctggttcc cccttcacgc acccgccgcc tgcacgaaca cttccaccac
    16741 atttcctggc agaccgaagc catcccgcaa accgaaagta agcccgacaa accctggttt
    16801 gcacttccac aaacatccga acggcaaaaa ccggaacaca tcctcgttat cggcgcgggc
    16861 atatccggcg cggcaaccgc ccacgcctta gcatcatacg gcatttccgt taccgtattg
    16921 gaagcccgaa aagccgccca agccgccagc ggcaaccgcc aagggctgct ctacgccaaa
    16981 atctcgccac acaataccga acaaaccgaa cttttgctca ccggctacgg ctacaccaaa
    17041 cgcctgctcg gacacatcct gcccgaatcc gaaacctggg gcggcaacgg catcatccac
    17101 ctcaattaca gccgcaccga acaacaacgc aatcacgaat tgggtttgca aaaacaccat
    17161 aaccacctct accgcagcat cacgcaggca gaagccgaaa aaatcgccgg tatcccactg
    17221 tccgtcccat acgaccaccc ttcatgcgga ctctactggc aacacggcgt atggctcaat
    17281 ccacccgcat tcgtccgcgc cctcctcaac catccgctca ttggactaca cgaagacaca
    17341 ccgttaaccg acatttccca cgacggggaa aagtggattg caagcacgcc aaacggcata
    17401 ttttccgcca cacacatcat ctactgcacc ggcgcgcaca gctcctgcct gcccgaaacc
    17461 aacctcgccg ccctgcctct caggcaaata cgcggacaaa ccggcctcac accatccacc
    17521 ccgttttccg aacaactgcg ttgcgccgtt tcaggcgaaa gctacatcag cccgtcgtgg
    17581 cacggactgc actgctacgg cgcgagtttt attcccaaca gcagccatac cggatggaac
    17641 gaagccgaag aagcctcaaa ccgccaagca ttggcacagc ttagccccgc cctcgccgaa
    17701 tcattgtttg ccgccaaccc aatcccccaa aaacaccaag ggcacgccgc catgcgctgc
    17761 gacagccccg accaccttcc cctagtcggc gcactcggcg acattgccgc tatgcaacaa
    17821 acttacgcca aactcgcgct ggacaaaaac tatcgcatcg acgccccctg cccgtacctg
    17881 cccaatgcct acgccaacac cgcccacggc acacgcgggc ttgccaccgc ccccatctgc
    17941 gccgccgccg ttgcagccga aatcctaggc ttgccccatc ccctctcaaa acgcctgcgc
    18001 cacgccctac accccaaccg caccatcatc cgcgccatcg tcagaaggaa ggatctaacc
    18061 ccttaagcct ttgccgcatg ccgtcccgac cacagaaaat catattcaaa taaacatacc
    18121 aatcctgacg gtaaaatatc gaaaacaccc ataacgtttt cttcccaaaa taaggagtaa
    18181 ggaatgccta acaaattgcc tttgctgtcc aagatgaata ttccgtaaca ttcgaagact
    18241 tccttaaccc tgccagccgt ttcggtttct gtaccccctg ttagataagg aaggaatgaa
    18301 aaagccctgt tgattgtatg aagtaaaggg cgttaatttt tgttaatctt cccttctcag
    18361 gaactcaata tataaggatg ttaattttta tttcccttct cgaggact
//
LOCUS       DAWFHY010000039        18145 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.039, whole genome shotgun sequence.
ACCESSION   DAWFHY010000039 DAWFHY010000000
VERSION     DAWFHY010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 18145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18145
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.039"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(230..1354)
                     /gene="mafB"
                     /locus_tag="WGQ68_001162"
                     /pseudo
     CDS             complement(230..1354)
                     /gene="mafB"
                     /locus_tag="WGQ68_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(1394..1519)
                     /locus_tag="WGQ68_001163"
                     /pseudo
     CDS             complement(1394..1519)
                     /locus_tag="WGQ68_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            complement(1848..2258)
                     /locus_tag="WGQ68_001164"
     CDS             complement(1848..2258)
                     /locus_tag="WGQ68_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714964.1"
                     /translation="MKIYNLDNIITSSALSLYKSTWVKLDLISCAKFIIEGTDFTFDW
                     DEGIENWIVFFHKNKVIGYLCAKLPFAILDNEQLKNQLYHKYPHVLTLQIKNIETEKI
                     SLNVNICRLTLCPSFHDEHDTLVCSVQDFYVNTV"
     gene            complement(2846..3229)
                     /locus_tag="WGQ68_001165"
     CDS             complement(2846..3229)
                     /locus_tag="WGQ68_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714965.1"
                     /translation="MMTEPHGYPSNNLPVKIINDIIYATQLVEDLVLGKIKIVDFLKS
                     YNNFYYWLGFDELPQSEKIKFLNYLNILSIHKEIQDKTVNRVYTDCFDIDKLHSLGRI
                     TTNECINGIKKIYQKNKLEFDNILK"
     gene            complement(3195..4640)
                     /gene="mafB"
                     /locus_tag="WGQ68_001166"
     CDS             complement(3195..4640)
                     /gene="mafB"
                     /locus_tag="WGQ68_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
                     /protein_id="HEZ0714966.1"
                     /translation="MKLSIQKFMMLFAAAISLLQIPISHANGLDARLRDDMQAKHYEP
                     GGKYHLFGDGRGSVKNRVYAVQTFDATAVGPILPITHERTGFEGVIGYETHFSGHGHE
                     VHSPFDHHDSKSTSDFSGGVDGGFTVYQLHRTGSEIHPEDGYDGPQGSDYPPPGGARD
                     IYSYYVKGTSTKTKTNIVPQAPFSDRWLKENASAASGFLSRADEAGKLIWENDPNQNW
                     WANRMDDIRGIIQGAANPFLTGFQGLGVGAITDSAVNPVTYAAARKTLQGIHNLGNLS
                     PEAQLAAATALQDSAFAVKDSINSARQWADAHPNITATAQTALSVAEAAGTVWRGKKV
                     ELNPTKWDWVKNTGYKKPAVRLMQTVDGEMAGGNKPPKPSTQQHSTHSDNNIGLPAPY
                     VKPDTSISPTGTIYVDSKGNAIPTPIGGYITGSPDARFIQVRDSNGNLTGARIDDAHH
                     ANTHQDPRALTGHAHVPGVTNDDGTPWLPIK"
     gene            complement(4680..5642)
                     /gene="mafA"
                     /locus_tag="WGQ68_001167"
     CDS             complement(4680..5642)
                     /gene="mafA"
                     /locus_tag="WGQ68_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0714967.1"
                     /translation="MQARLLIPILFSVFILSACGTLTGIPSHGGGKRFAVEQELVAAS
                     ARAAVKDMDLQALHGRKVALYIATMGDQGSGSLTGGRYSIDALIRGEYINSPAVRTDY
                     TYPRYETTAETTSGGLTGLTTSLSTLNAPALSRTQSDGSGSKSSLGLNIGGMGDYRNE
                     TLTTNPRDTAFLSHLVQTVFFLRGIDVVSPANADTDVFINIDVFGTIRNRTEMHLYNA
                     ETLKAQTKLEYFAVDRTNKKLLIKPKTNAFEAAYKENYALWMGPYKVSKGIKPTEGLM
                     VDFSDIRPYGNHTGNSAPSVEADNSHEGYGYSDEVVRQHRQGQP"
     gene            complement(5826..6545)
                     /locus_tag="WGQ68_001168"
     CDS             complement(5826..6545)
                     /locus_tag="WGQ68_001168"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEZ0714968.1"
                     /translation="MTQQIKYKRVLLKLSGESLMGSDPFGINHDTIVQTVGEIAEVVK
                     MGVQVGIVVGGGNIFRGVSAQAGSMDRATADYMGMMATVMNALALKDAFETLGIKARV
                     QSALSMQQIAETYARPKAIQYLEEGKVVIFAAGTGNPFFTTDTAAALRGAEMNCDVML
                     KATNVDGVYTADPKKDSSATRYETITFDEALLKNLKVMDATAFALCRERKLNIVVFGI
                     AKEGSLKRVVTGENEGTLVHC"
     gene            complement(6763..7617)
                     /locus_tag="WGQ68_001169"
     CDS             complement(6763..7617)
                     /locus_tag="WGQ68_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003795802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEZ0714969.1"
                     /translation="MAEITAKMVADLRAATGLGMMECKKALVEAEGNFDKAEEILRIK
                     SGAKAGKLAGRTAAEGVLAYAINGNVGALVEVNCETDFVAKDAGFVEFANFVAKTAAE
                     KKPASVEELSELVEAERKAIIAKLGENMSVRRFQVIDTANQLVAYIHGALATEGVLVE
                     YKGSEDVARKIGMHIVAAKPQCVSEAEVDAETVEKERHIYTEQAIASGKPADIAAKMV
                     EGRIRKFLAEITLNGQAFVMNPDQTVAQFAKENGTEVVSFVRYKVGDGIEKAVVDYAA
                     EVAAAAKV"
     gene            complement(7746..8474)
                     /gene="rpsB"
                     /locus_tag="WGQ68_001170"
     CDS             complement(7746..8474)
                     /gene="rpsB"
                     /locus_tag="WGQ68_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEZ0714970.1"
                     /translation="MSQITMRQMIEAGVHFGHQTRFWNPKMAQYIFGARNKIHIVNLE
                     KTLPMFQDAQEAVRRLVANKGTVLFVGTKRQARDIIREEATRAGMPFVDYRWLGGMLT
                     NYKTVKQSIKRLEEKTAALENAAESGFSKKEILEMQRDVEKLERSLGGIKNMKGLPDA
                     IFVIDTGYQKGTLVEAEKLGIPVIAVVDTNNSPDGVKYVIPGNDDSAKAIRLYCRGIA
                     DAVLEGKNQALQETVAAAQEAAAE"
     gene            complement(8651..8884)
                     /locus_tag="WGQ68_001171"
     CDS             complement(8651..8884)
                     /locus_tag="WGQ68_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714971.1"
                     /translation="MWHIVAIGYLFVAVMYSAAQPSIARALIYLVFWAVLPTVFTVFV
                     VTVRRRNRLMKQQEQAEFEQQRAQRQKDSGTKP"
     gene            8962..9543
                     /locus_tag="WGQ68_001172"
     CDS             8962..9543
                     /locus_tag="WGQ68_001172"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEZ0714972.1"
                     /translation="MRNEEKRALRRELRGRRSQMGRDVRAAATVKINHLLKRYIKKGR
                     KIGVYWPMGKELRLDGFVRAAQKRGVKLYLPYIEPRSRRMWFTPYPESGMERERIRGR
                     ARLNVPQFAGRKIRVHGLSVLLVPLVGIDREGYRLGQAGGYYDATLAAMKYRLQAKTV
                     GVGFACQLVDRLSVEAHDLPLDGFVSEAGILCF"
     gene            9604..10194
                     /locus_tag="WGQ68_001173"
     CDS             9604..10194
                     /locus_tag="WGQ68_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0714973.1"
                     /translation="MSEKIILPVLSLGGVRLEPLDVHHETGLREAVCDGEVWKLGVTS
                     APHPDRVADYIGTALATRLAFAVVDEEAGRVVGTTAYYHFEPQIPRLDIGFTWYAASA
                     RRTRINTCCKIMLLDYAFDVLACRCVGWRTDILNLASQRAIERLGAEKDGVLRMHMLR
                     KDGSVRDTVVYSMLREDWCKNREILTGRLAGYGVQV"
     gene            10732..12198
                     /locus_tag="WGQ68_001174"
     CDS             10732..12198
                     /locus_tag="WGQ68_001174"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HEZ0714974.1"
                     /translation="MAEATDVVLVGGGIMSATLGVLLKELEPSWEITLIERLEDVALE
                     SSNAWNNAGTGHSALCELNYAPLGANGIIDPARALNIAEQFHVSRQFWATLVAEGKLE
                     DNSFINAVPHMSLVMNEDHCSYLQKRYDAFKTQKLFENMEFSTDRNKISDWAPLMMRG
                     RDENQPVAANYSAEGTDVDFGRLTRQMVKYLQGKGVKTEFNRHVEDIKRESDGAWVLK
                     TADTRNPDGQLTLRTRFLFLGAGGGALTLLQKSGIPEGKGYGGFPVSGLFFRNSNPET
                     AEQHNAKVYGQASVGAPPMSVPHLDTRNVDGKRHLMFGPYAGFRSNFLKQGSLMDLPL
                     SIHMDNLYPMLRAGWANMPLTKYLLGELRKTKEERFASLLEYYPEANPDDWELITAGQ
                     RVQIIKKDSEKGGVLQFGTEIVAHADGSLAALLGASPGASTAVPLMIRLMHQCFPERA
                     PSWEGRLKELVPGYGIKLNENPERADEIIAYTAKVLDI"
     gene            complement(12386..12760)
                     /locus_tag="WGQ68_001175"
     CDS             complement(12386..12760)
                     /locus_tag="WGQ68_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HEZ0714975.1"
                     /translation="MKLLTTAILSSAIALSSMAAAAGTNNPTVAKKTVSYVCQQGKKV
                     KVTYGFNKQGLTTYASAVINGKRVQMPVNLDKSDNVETFYGKEGGYVLGTGVMDGKSY
                     RKQPIMITAPDNQIVFKDCSPR"
     gene            complement(12998..13297)
                     /locus_tag="WGQ68_001176"
     CDS             complement(12998..13297)
                     /locus_tag="WGQ68_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEZ0714976.1"
                     /translation="MKIFENIEDVKAIRKKTGLNQIDFWGKVGVTQSGGSRYETGRKM
                     PKPVRELLRLVHIECIDLAKVNKKDMEIAALLKKHHPDLYAELSKQTKSERKKQS"
     gene            complement(13452..14231)
                     /gene="map"
                     /locus_tag="WGQ68_001177"
     CDS             complement(13452..14231)
                     /gene="map"
                     /locus_tag="WGQ68_001177"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEZ0714977.1"
                     /translation="MNGIIIKTPEEIEKMRELGKLVAEALDYIGQFVKPGVTTDEIDK
                     LVYDYHVNVQGGYPAPLHYGNPPYPKSCCTSVNHVICHGIPDDKPLKEGDIINIDLTI
                     KKDGFHGDSSRMFTVGKVSPIAQRLIDVTHASMMAGIEAVKPGATLGDVGYACQQVAE
                     NAGYSVVQEFCGHGIGRGFHEAPQVLHYGKKGQGPVLKPGMIFTVEPMINQGKRHLRI
                     LNDGWTVVTKDRSLSAQWEHEVLVTETGYEILTVSPASGKP"
     gene            complement(14271..14921)
                     /locus_tag="WGQ68_001178"
     CDS             complement(14271..14921)
                     /locus_tag="WGQ68_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714978.1"
                     /translation="MPSEPPSDGIARHPKSTIKMAKKPNKPFRLTPKLLIRVVLLICI
                     TAIGALAIGIVSTFNPNGGKTLQTEPQHTDSPRETEFWLPNGVVGQDAAQPEHHHAAS
                     SEPAQPDGTDESGSGLPSPAAPKKNRGKPQPADTAQTDRQPDDAGTQAENTLKETPVL
                     PTNVPRPEPRKETPEKQAQPKETPKEKETPKENHTKPDTPKNTPPKPHKEILDNLF"
     gene            complement(14929..15537)
                     /locus_tag="WGQ68_001179"
     CDS             complement(14929..15537)
                     /locus_tag="WGQ68_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEZ0714979.1"
                     /translation="MKPKPHTVRTLIAAIFSLALSGCVSAVIGSAAVGAKSAVDRRTT
                     GAQTDDNVMALRIETTARSYLRQNNQTKGYTPQISVVGYNRHLLLLGQVATEGEKQFV
                     GQIARSEQAAEGVYNYITVASLPRTAGDIAGDTWNTSKVRATLLGISPATQARVKIVT
                     YGNVTYVMGILTPEEQAQITQKVSTTVGVQKVITLYQNYVQR"
     gene            complement(15601..16194)
                     /locus_tag="WGQ68_001180"
     CDS             complement(15601..16194)
                     /locus_tag="WGQ68_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoheptose isomerase"
                     /protein_id="HEZ0714980.1"
                     /translation="MTTLQERVAAHFAESIRAKQEAEKVLVEPTVQAAELMLQCLMND
                     GKILACGNGGSAADAQHFAAEMTGRFEKERMELAAVALTTDTSALTAIGNDYGFNHVF
                     SKQVRALGRAGDVLVGISTSGNSANVIEAVKAAHERDMHVIALTGRDGGKIAAMLKDT
                     DVLLNVPYPRTARIQENHILLIHAMCDCIDSVLLEGM"
     gene            complement(16199..16546)
                     /locus_tag="WGQ68_001181"
     CDS             complement(16199..16546)
                     /locus_tag="WGQ68_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEZ0714981.1"
                     /translation="MRLNHKQGEAGEDAALAFLQSQGCTLLARNWHCAYGEIDLIVKN
                     GGMILFVEVKYRKNRQFGGVAYSISPSKLLKLQRSVEYYLQQNRLTNVPCRLDAVLIE
                     GNRPPEWIQNITG"
     gene            16592..17467
                     /gene="rsmI"
                     /locus_tag="WGQ68_001182"
     CDS             16592..17467
                     /gene="rsmI"
                     /locus_tag="WGQ68_001182"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEZ0714982.1"
                     /translation="MFQKHLQKASDSVVGGTLYVVATPIGNLADITLRALAVLQKADI
                     ICAEDTRVTAQLLSAYGIQGKLVSVREHNERQMADKIVGYLSDGMVVAQVSDAGTPAV
                     CDPGAKLAGRVREAGFKVVPVVGASAVMAALSVAGVEGSDFYFNGFVPPKSGERRKLF
                     AKWVRAAFPIVMFETPHRIGATLADMVELFSERRLMLAREITKTFETFLSGTVGEIQT
                     ALSADGNQSRGEMVLVLYPAQDEKHEGLSESAQNIMKILTAELPTKQAAELAAKITGE
                     GKKALYNLALSWKNK"
     gene            complement(17502..17907)
                     /locus_tag="WGQ68_001183"
                     /pseudo
     CDS             complement(17502..17907)
                     /locus_tag="WGQ68_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215060.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(18026..18136)
                     /locus_tag="WGQ68_001184"
                     /pseudo
     CDS             complement(18026..18136)
                     /locus_tag="WGQ68_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003677171.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
BASE COUNT         4027 a         4187 c         4830 g         5101 t
ORIGIN      
        1 gattatttgg caatggattg ccttcataat cttgtcgttg cggatatttt ccctgtacac
       61 catcggttcg cggcactctt tgggttctta taacctctcc atttggacga atatctattt
      121 tcacgccatc aggacctttc catgtttcat attgagccgc atctgaacca gtacgtctaa
      181 aaccttgttc ttgcaatgct tgacgtaatt catctcgcgt agttggtctg atagatttaa
      241 caactggctt gtttccacct gccatctctc catctaaagt ctgcataggt cgggcagcag
      301 gtgttttata gccggtattt ttaacccaat cccatttagt cgggttaagt tctacttttt
      361 tacctctcca aaccgtacct gcggcctctg ctgcggaaag ggcagtttgg gctgttgcag
      421 ttatattcgg atgggcatca gcccattgtc tggcagagtt gataccgtct tttaccgcaa
      481 aagcactgtc ttgtaatgcg gttgcggccg caagttgtgc ttcgggactt aaatttccta
      541 aatgattaat accttgtaga gtctgctgcg cggctgtatc tgtgactggg cttactgcac
      601 tgtctgtaat tgccccaatc cctactcctt gaaaacccat taaaaaagga ttaaccgcac
      661 cttggacgat gccgcgaata tcatccatac ggttagccca ccaattttta ttggggtcgc
      721 tttcccatat cagttttcct gcttcatccg cacggctgaa aaaaccagag gcggcaccgg
      781 cattttcttt tagccaacgg tctgaaaatg gggcttgagg gacaatatta gtctttgttt
      841 ttgttgaagt tcctttgaca taatagctgt atatatccct tgctcctccg gggggcggat
      901 aatcgctgcc ttgcggcccg tcatatccat cctccggatg aatttccgac cctgttcgat
      961 gaagttggta aacagtaaaa ccaccgtcta caccgccgct gaaatcagaa gtgctttttg
     1021 aatcatgatg atcgaacgga ctgtgtactt catatccgtg tcctgaaaaa tgggtttcat
     1081 aaccgataat gccttcaaac cctgtccgtt cgtgtgtaat aggcagtaca ggactgaccg
     1141 cagttgcatc aaatgtttgg acggcgtaaa cccgattttt aacactgccg cgagcattac
     1201 caaacagatg gtatttacca cccggttcgt agtgttttgc ctgcatatca tcgcgtaaac
     1261 gggcatccaa accgttcgca tgactaatgg ggatttgcag caacgatatt gctgctgcaa
     1321 acagcatcat gaatttttga ataggcaatt tcattttgtt ttccttggta gcaagcggtt
     1381 atggtagtgt gaatcaaggt tgcccttgtc tatgttgtcg cactacttca ttgctgtatc
     1441 catacccctc atgactgtta tcagtctcta cggatggggc ggagttaccc gtatgattgc
     1501 cgtatggccg gatacttgta tccgacaaaa atatttgacg catctatcgt ttccgaaatg
     1561 ccgctgttga aaaatgtcgg attcgagaat ccgacctacg gttgctgaac aatgttttgt
     1621 agaacttgct caaaatccat gtttcgccat tttcctttat atatacctaa tacagcacgg
     1681 gttacgattt cgtttaagtc ataaatagcc atttacgtcc tctgctaatt ttcagcgagt
     1741 agattgggtt accaaaccca acaaaaaagc agcctgaaaa atttcaggat gcttgtttta
     1801 tgttttttat caatgaatta ggttttaatc cagcctgcgc ttgctagtta aacagtattt
     1861 acataaaaat cttgtactga acaaacaagc gtatcatgtt catcatgaaa tgatgggcat
     1921 aacgttaatc tacaaatgtt tacatttaaa gatatttttt cagtttcaat atttttaatt
     1981 tgtaaggtta atacatgagg atatttgtga tatagctgat tttttaattg ttcgttgtct
     2041 aatatagcaa aaggtaattt tgcacacaaa taaccaataa ctttgttttt atgaaaaaaa
     2101 actatccaat tttcaattcc ttcatcccaa tcaaaagtaa aatccgttcc ttctatgata
     2161 aatttagcac aagatattaa atctaactta acccaagtgg atttatacag actcaatgct
     2221 gatgaagtta taatattatc taaattataa attttcataa atcaacgttc cttacctggt
     2281 gttatatgca caggtctaat cccatcttta ttcggctttc tacgataaat gttaattata
     2341 tgtgtcggtt ttccgtctgt atagccaact acgtcgttcg ttggaacttt tgccttattt
     2401 tgactatccc ataaattatg tttatctgca tatctagcag catctaatgt agctttttcc
     2461 gcatcaacat ctgatttaaa taaacttttg ctatctatct tgtctagact agatgcatca
     2521 ggtttgtgtc gtaaaaagca ctgtcctgta ataggctcgc ggcagcaagt tgtgcttccg
     2581 gacttaattt tcctaaatca ttaatacctt gtaaagtttg ctgcgcagag atgtaggtcg
     2641 gatatttgta tccgacaaaa atatttgacg catctatcgt ttccgaaatg ccgctgttgg
     2701 aaaatgtcgg attcgagaat ctgacctacg gctgctgact attttcttgt ttatataatt
     2761 ctacaactgc aaactactat caaatctttt tatctctggc tctacactca gcaagaatat
     2821 ttttatttta caaaacatag gctagttatt tgagtatatt gtcaaattct aatttgtttt
     2881 tttgatagat tttttttata ccattaatac attcattagt tgtaattctg ccaagcgaat
     2941 gtaatttatc aatatcaaaa caatcggtat aaaccctatt cacagtttta tcttgtattt
     3001 ccttatgaat acttaatata ttgagatagt ttagaaattt tattttctca gattgaggta
     3061 actcatcaaa accaagccaa taataaaaat tgttatatga ttttaaaaaa tcaacaattt
     3121 ttatttttcc taaaaccaaa tcttcaacta actgggttgc ataaataata tcatttatga
     3181 ttttaacagg taaattattt gatgggtaac catggggttc cgtcatcatt tgtaactcct
     3241 ggtacatgtg cgtgtcctgt aagtgcgcgt gggtcttgat gggtatttgc atgatgagca
     3301 tcatctattc tggctccagt caagtttcca ttactatctc ggacttggat aaaacgagca
     3361 tcaggcgaac ctgttatata accgcctatt ggtgttggta ttgcatttcc tttgctgtct
     3421 acataaattg ttcctgtcgg agaaatagat gtatcaggtt taacatatgg ggcaggtaag
     3481 cctatattgt tatcagagtg tgtagagtgt tgctgcgtac ttggtttcgg tggcttattt
     3541 cccccagcca tctccccgtc tacagtctgc ataaggcgaa cagcaggttt tttatagccg
     3601 gtatttttaa cccaatccca tttggtcggg ttaagttcta cttttttacc tctccaaacc
     3661 gtacctgcgg cctctgctac ggaaagggca gtttgggcag tagctgtaat gttcggatgg
     3721 gcatcagccc attgtctggc agagttgata ctgtctttta ccgcaaaagc actgtcttgt
     3781 aatgcggttg cggccgcaag ttgtgcttcc ggacttaaat ttcctaaatt gtgaataccc
     3841 tgtaaagttt tccgtgctgc cgcataggtt accgggttta ccgcactgtc tgtaattgcc
     3901 ccaactccca atccctgaaa acccgttaga aaaggattgg ctgcaccttg gatgatgccg
     3961 cgaatatcat ccatacggtt agcccaccaa ttttgattgg ggtcgttttc ccatatcagt
     4021 tttcctgctt catccgcacg gctgagaaaa ccagaggcgg cactggcatt ttcttttagc
     4081 caacggtctg aaaatggggc ttgagggaca atattagtct ttgtttttgt tgaagttcct
     4141 ttgacataat agctgtatat atcccttgct cctccggggg gcggataatc gctgccttgc
     4201 ggtccgtcat atccatcctc cggatggatt tccgaccctg tccgatgaag ttggtaaaca
     4261 gtaaaaccac cgtctacacc gccgctgaaa tcagaagtgc tttttgaatc atgatgatcg
     4321 aacggactgt gtacttcatg tccgtgccct gaaaaatggg tttcatagcc gataacacct
     4381 tcaaatcctg tccgttcgtg tgtaataggc agtatggggc cgaccgcagt tgcatcaaat
     4441 gtttggacgg cgtaaacccg atttttaaca ctgccgcgac catcgccgaa cagatggtat
     4501 ttaccacccg gttcgtagtg ttttgcctgc atatcatcgc gtaaacgggc atccaaaccg
     4561 ttcgcatgac taatggggat ttgcagcaac gatattgctg ctgcaaacag catcatgaat
     4621 ttttgaatag acaatttcat tttgttttcc ttggtagcaa gcggttatgg tagtgtgaat
     4681 caaggttgcc cttgtctatg ttgtcgcact acttcatcgc tgtatccata cccctcatga
     4741 ctgttatcag cctctacgga tggggcggag ttacccgtat gattgccgta tggtcggata
     4801 tcggagaaat cgaccattaa tccttccgtc ggtttaattc ctttgcttac tttatacggc
     4861 cccatccaca atgcgtaatt ttctttatag gcagcttcaa acgcattggt tttgggtttg
     4921 atgagcaatt ttttattggt tctgtctact gcgaaatatt ccagttttgt ttgggctttc
     4981 agtgtttcgg cattgtatag gtgcatttcg gttctgttgc gtatcgttcc gaatacgtcg
     5041 atgttaataa acacatctgt atcggcattg gcaggagaaa caacgtctat gccgcgcagg
     5101 aaaaatacgg tctgtaccaa gtgggaaaga aaggcagtgt cgcgcgggtt agtcgtcaag
     5161 gtttcatttc gataatcccc catcccgcca atatttaagc ccagactgct tttacttccg
     5221 ctaccgtctg attgggtgcg agagagtgca ggggcattaa gtgtagataa agaagtggtt
     5281 aaacctgtca aaccgcctga tgttgtttca gcggtggttt cgtaacgtgg ataggtgtaa
     5341 tcggtacgga cggcagggct gtttatgtat tcgccacgaa tcagtgcatc aatggagtag
     5401 cgaccccctg tcaaactgcc tgaaccttgg tcgcccatag ttgcaatgta caatgcaact
     5461 tttcgtccgt gtaatgcctg taaatccatg tctttaacgg cagctctggc agaagcggcc
     5521 acaagttctt gttcgaccgc aaagcgttta cctccgccat gcgatggaat acctgtcagt
     5581 gtcccgcagg cggataaaat aaaaactgaa aaaagaatag gtatcagcag ccgtgcttgc
     5641 atagattttc tcctttgatg aaaaacaaat tgtatcaaaa ttgtaaatat agtggattaa
     5701 atttaaacca gtacggcaag gcgaggcaac gccgtactgg tttaaattta atccactata
     5761 atctccatat aataaatggc tgtctgaagc ccatatgcga ctatatctgc cgacactatg
     5821 gtcaatcagc agtgaaccag cgtaccttcg ttttcaccgg ttactacacg tttgagcgag
     5881 ccttctttag caatgccgaa tactacaata ttgagcttgc gttcgcggca gagggcgaaa
     5941 gcggtggcat ccatcacttt gaggtttttc aacaaggctt cgtcaaaagt aatggtttcg
     6001 tagcgcgtgg cggatgagtc ttttttgggg tctgcggtgt acacgccgtc gacgttggtg
     6061 gctttaagca tcacgtcgca gttcatttcc gcaccgcgca atgcggcggc agtgtcggtc
     6121 gtgaagaacg ggttaccggt accggcggca aaaatcacga ctttgccttc ttccaaatat
     6181 tggatggctt taggacgggc gtaagtttca gcgatttgct gcatagacag tgcggattgt
     6241 acgcgcgctt tgatgcctaa agtttcaaat gcgtctttga gtgccaacgc gttcatcacg
     6301 gtcgccatca tgcccatgta gtcggcggtg gcgcgatcca tgctgcctgc ttgggcggat
     6361 acgccccgga aaatattgcc gccgccgaca acaataccga cttgcacgcc cattttaacg
     6421 acttcggcaa tttcgccgac ggtttggacg atggtgtcgt gattgatgcc gaacggatcg
     6481 gaacccatca gggattcgcc ggagagtttc agtaatacgc gtttgtattt gatttgctgt
     6541 gtcatgggat accttgcttt cttgaatgtc gtcggtcgga gttgggccgt ctgaatttcc
     6601 aacggtcgga atattaatga tagcgtgtgt cggtttatta ttgttttgtt ttaagacggt
     6661 ctgaatattc ggtcgtctta aaataggtac cgcaaacggt tttctcaaaa aaaagcaccc
     6721 tgattcgttt ggaatccagg tgctttcttt ttcataagtg ctttacactt tggcggctgc
     6781 ggctacttcg gctgcgtagt cgacaacggc tttctcgata ccgtcgccta ctttgtagcg
     6841 gacgaagctg accacttcag tgccgttttc tttggcgaat tgggcaacag tttgatcggg
     6901 gttcatcacg aatgcttggc cgttcagagt gatttcagcc aagaatttgc gaatacggcc
     6961 ttcaaccatt ttagcggcga tgtcggcagg tttgccggaa gcgatggctt gttcggtgta
     7021 gatgtggcgt tctttttcaa cggtttcggc atctacttcg gcttcgctta cgcattgtgg
     7081 tttagcggca acaatgtgca taccgatttt gcgtgctacg tcttcagagc ctttgtactc
     7141 aaccaatacg ccttcggtcg ccaatgcgcc gtggatgtag gcaaccagtt ggttggcagt
     7201 gtcgatcact tggaagcgac ggacagacat gttctcgccc aatttggcga tgatggcttt
     7261 gcgttctgct tcaaccagtt cgctcagttc ttcaacagaa gccggttttt tctcggcggc
     7321 ggttttcgca acgaagttgg caaattctac gaagcccgcg tctttcgcaa cgaagtcggt
     7381 ttcgcagttt acttcgacca atgcgccgac attgccgttg atcgcgtaag ccaatacgcc
     7441 ttcggcagcg gtacggccgg ccagtttacc ggctttcgca ccggatttga tacgcaggat
     7501 ttcttcggct ttgtcgaagt tgccttcggc ttcaaccaag gcttttttgc attccatcat
     7561 gcccaggccg gtagcggcgc gcagatcggc aaccattttt gcagtaattt ctgccatttt
     7621 gaatctccta gatttttggg aaatacggaa aggattttcc gcattcggga atcgtgtgcc
     7681 gcgaacggac gttcagacgg catatttgag aaaaggggca taacgcccct cttcggtttg
     7741 ccggattact cggcagcggc ttcttgggca gcggctacgg tttcttgcag cgcttggttt
     7801 ttgccttcca aaactgcgtc agcgatgccg cggcagtaca ggcggatggc tttggcggag
     7861 tcgtcgttgc cggggatgac gtatttcacg ccgtcagggc tgttgttggt atcgactacg
     7921 gcgataacgg ggatgcccag tttttcagcc tcgaccagag tacctttttg gtagccggta
     7981 tcgataacga aaatcgcgtc gggcaggcct ttcatgtttt tgataccgcc caaagaacgt
     8041 tccagttttt caacatcgcg ttgcatttcc agaatttctt ttttgctgaa accgctttcg
     8101 gcagcatttt ccaaggctgc ggttttttct tccaggcgtt tgatggattg cttaacggtt
     8161 ttgtagttgg tcagcatacc gcccaaccag cggtaatcaa cgaaaggcat accggcacgg
     8221 gtcgcttctt cgcggatgat gtcgcgggct tggcgtttgg tacctacgaa caatactgta
     8281 cctttgttgg caaccaaacg gcgtacggct tcttgcgcgt cttggaacat cggcagggtt
     8341 ttttccaagt ttacgatatg gattttgttg cgtgcgccga aaatgtattg tgccattttc
     8401 gggttccaga aacgggtttg gtggccgaag tggacaccgg cttcaatcat ctgacgcata
     8461 gtaatttgag acattttgat ttccttgaaa gggttaaggg cacacattca atcgcataga
     8521 acttgatagg cgatgcctat tgtcaagcac ccttcggcga aagtgggcat aagttttaaa
     8581 aaataaaaga gtgtttccga aatggaaaac tgacggatta tagcggataa gaggccgtct
     8641 gaaaagggat tcagggtttt gtgccgctgt ctttttgccg ttgtgcacgc tgctgttcga
     8701 attctgcctg ttcctgctgc ttcatcaagc ggttgcggcg gcggacggtt acgacgaaga
     8761 ccgtgaacac ggtgggcaga accgcccaaa aaaccaaata aatcaaggcg cgcgcaatac
     8821 tcggctgcgc ggcggaatac ataacggcaa caaaaagata gccgatggcg acgatgtgcc
     8881 acattgtgtg cgtcctgttt gaatgttaac aaaatcgtta taatcgcaac attctaccgc
     8941 aatcctgaaa ggccgtctga aatgaggaac gaggaaaaac gcgccctgcg ccgcgaattg
     9001 cgcgggcggc gttcgcaaat ggggcgggac gtgcgggcgg cggcaacggt aaaaatcaac
     9061 cacctgctca aacgttatat taaaaaaggg cggaaaatcg gcgtgtattg gccgatgggc
     9121 aaggaattgc gtttggacgg ctttgtccgc gcggcgcaaa aacgcggtgt aaaactttat
     9181 ctgccttata tcgaaccgcg ttcgcggcgg atgtggttta cgccgtatcc tgaaagcgga
     9241 atggaacggg aacgcatacg gggcagggcg aggttgaacg tgccgcagtt tgcagggcgc
     9301 aaaatccgcg tgcacggttt gtcggtattg ctcgttccgc ttgtcggcat agaccgcgaa
     9361 ggctaccgtt tggggcaggc aggcggctat tacgatgcga cgcttgcagc gatgaaatac
     9421 cgtttgcagg caaaaaccgt gggcgtgggc tttgcctgcc agttggtgga cagactgtcg
     9481 gtcgaggcgc acgacctgcc gctggacggt tttgtgtcgg aggcggggat attgtgcttt
     9541 tagccgttca gacggcattt cggtcggaaa tgccgtctga aggtggaata ttgggagaaa
     9601 aggatgtcgg aaaagataat actgccggtc ttgtcgttgg gtggcgtacg gctcgaaccg
     9661 ttggatgtgc atcatgaaac gggtttgcgc gaggcggttt gcgatgggga agtttggaag
     9721 ctgggagtaa cgtccgcgcc ccatccggat agggtggcag actatatcgg gacagcattg
     9781 gcaacgcgtt tggcgtttgc ggttgtcgat gaagaggcgg gcagggtggt cgggacaacg
     9841 gcgtattatc attttgaacc gcagattccg cgtttggata ttggattcac atggtatgcg
     9901 gcatcggcac ggcggacacg gataaacacc tgttgcaaaa tcatgctgtt ggattacgca
     9961 tttgatgttt tggcttgccg ttgcgtggga tggcgcacgg atattctgaa ccttgcctcg
    10021 caacgtgcca tcgaacggct gggtgcggaa aaagacggcg tgctgcgtat gcatatgctc
    10081 cgtaaagacg gcagtgtgcg cgatacggtc gtgtacagta tgctgaggga agattggtgc
    10141 aaaaacaggg aaatcctgac cggcaggttg gcagggtatg gcgtgcaggt gtgaggcagt
    10201 attttcaaat ttatagtgga ttaacaaaaa ccagtacagc gttgcctcgc cttagctcaa
    10261 agagaacgat tctctaaggt gctgaagcac caagtgaatc ggttctgtac tatttgtact
    10321 gtctgcggct tcgtcgcctt gtcctgattt ttgttaatcc actataaaaa acttaacgca
    10381 atatgctgac aatcttttaa aaattaatat ataaacaatt ttgatttttt atctattaaa
    10441 aatttgatat atatagataa ataactattt ttatttttca tttcgcagtt tcccgacttg
    10501 gcgggcgaca gaaaaacttt gcatttccca gtaaaatcca ctacattcac gataacatat
    10561 tacaagcccc tgaagcggtg tcggaagcca tacttgcaac gcattccaac cccgaaacag
    10621 catactcgga cacacaccat tgtcggtttt ccctgctttt gcgattcagg cgtttgtagt
    10681 acggcaaata accataaggg cagaagatgc ctgttttttt aggagtaaac catggctgaa
    10741 gcgacagacg ttgtcttggt gggcggcggc attatgagcg cgactttggg cgttttgctc
    10801 aaagaactcg aaccgtcttg ggaaatcacc ctgattgaac gcttggaaga tgtggcgttg
    10861 gaatcgtcaa acgcgtggaa caacgccggc acggggcatt ccgcgctgtg cgaattgaac
    10921 tatgcgccgt tgggtgcaaa tgggattatc gatccggcgc gcgccctcaa tattgccgaa
    10981 cagtttcatg tcagccgcca gttttgggcg acgctggtcg cggaaggcaa gttggaagac
    11041 aattccttca tcaatgccgt gccgcatatg tctttggtga tgaatgaaga ccattgttct
    11101 tatcttcaaa aacgttatga cgcgtttaaa acccaaaaac tttttgaaaa tatggaattt
    11161 tccaccgatc ggaacaaaat ttccgattgg gctccgctga tgatgcgcgg ccgggacgaa
    11221 aaccaacccg tcgccgccaa ctactccgcc gaaggtacgg atgtcgattt cggacggctg
    11281 acgcgccaaa tggtgaaata tttgcagggc aagggcgtaa aaaccgagtt caaccgccac
    11341 gtcgaagaca tcaaacgcga atccgacggc gcgtgggtgc tcaaaaccgc cgatacccgc
    11401 aaccccgacg ggcagctcac cctccgtacc cgcttcctct tcctcggcgc gggcggcggc
    11461 gcgctgaccc tgctgcaaaa atccggcatc cccgaaggca aaggctacgg cggcttcccc
    11521 gtgtccggcc tgttcttccg caacagcaac cccgaaactg ccgaacaaca caacgccaaa
    11581 gtgtacgggc aggcttccgt cggcgcgccg ccgatgtccg tcccgcacct cgacacacgc
    11641 aacgtggacg gcaaacgcca ccttatgttc ggcccttacg caggcttccg ttccaacttc
    11701 ctcaagcaag gctcgcttat ggatttgccg ctgtccatcc atatggacaa cctctatcct
    11761 atgctgcgcg ccggctgggc gaatatgccg ctgaccaaat acctgctggg cgaattgcgt
    11821 aaaaccaaag aagaacgctt cgcctccctg ctggaatact accccgaggc aaaccccgac
    11881 gactgggaac tcatcaccgc agggcaacgc gtccaaatca ttaaaaaaga ctccgaaaaa
    11941 ggcggcgtgc tccagtttgg tacggagatt gtcgcccacg ccgacggctc gctcgccgca
    12001 ttgctgggcg cgtcgccggg cgcatcgacc gctgtgccgc tgatgatccg gctgatgcac
    12061 caatgcttcc ccgagcgcgc cccgtcttgg gaaggccgtc tgaaagagct ggtaccgggt
    12121 tacggcatca agttgaacga aaaccctgaa agggcggatg aaattatcgc ctataccgcg
    12181 aaagtgttgg atatttaagc cgaatctttc aagaaaagcc gtacccccat atggtgttga
    12241 cgccgtccct gccagttatg cccttcatcc ggcagggcag gttttgatac acggggcagg
    12301 taatcgtttt ttcccttttg cgaataatgg aacggtcaaa aaaacagcgt tttcatttct
    12361 gaaaacgctg ttttttgttg gcttgttaac gtggggaaca gtctttgaag acgatttggt
    12421 tgtcaggtgc ggtaatcata ataggctgtt tgcgatagga tttgccatcc atcacgccgg
    12481 tacccaaaac ataaccgcct tctttgccgt agaatgtttc cacattgtcg gatttgtcca
    12541 aattgacagg catttgcaca cgtttgccgt tgatgacggc ggaagcgtat gtggtcaggc
    12601 cctgtttgtt aaagccgtag gttactttga cttttttacc ttgctggcag acgtagctga
    12661 cggttttttt ggcaacggtg gggttgttcg tgccggcagc agcagccata ctgctgagcg
    12721 cgattgcgga agacaggatt gcggtggtca gaagtttcat ttttattcct ttggatggat
    12781 gtggagtttg cccgtttcgg acggtttgac attctaatgt cggcgtatcc tgaaataaac
    12841 aggaaattgg ataaagtttg taaaaaggcg ggttcgggcg gttcggcggt ttgaggcagg
    12901 ggcggcaggc gggatacggc gacctgtccg gtgtttcaga cggcattgtt tgcgtttttc
    12961 ggaaatgaaa aactgtcggg catccggagg ttgcggttta actttgtttt tttctttcgg
    13021 acttggtctg tttggacaac tcggcataca ggtcgggatg gtgttttttc aacagggcgg
    13081 cgatttccat atcttttttg ttgactttcg ccaaatcgat gcattcgata tggacgaggc
    13141 ggagcagttc gcgtacgggt ttgggcattt tgcggccggt ttcgtagcgc gagcctccag
    13201 actgggtaac gccgaccttg ccccagaagt ctatctggtt cagcccggtc tttttacgga
    13261 tggctttaac atcttctata ttttcaaata ttttcataaa acttccttta agtttgtctg
    13321 gttgcggggc aaattccgat ttgctttgtt ttcgtaatgt gccgggtcaa gcctgttttt
    13381 atattatata tcatatctgc cgtttctttc agacggcatt gtttgttttt atggggcgga
    13441 tacgtccggt ttcagggttt gccggaggcg gggctgacgg tgaggatttc gtagccggtt
    13501 tcggtcacca agacttcgtg ttcccattgg gcggagaggg agcggtcttt ggtaaccacc
    13561 gtccagccgt cgttgaggat acgcaggtgg cgtttgcctt ggttgatcat cggttcgacg
    13621 gtaaaaatca tacccggttt tagaacgggg ccctgtcctt tttttccgta gtgcaacact
    13681 tgcggggctt cgtggaaacc gcgcccgatg ccgtgtccgc agaattcctg tacgacggaa
    13741 tagccggcgt tttcggcaac ctgttggcag gcgtaaccta cgtcgcccag tgtcgcgccg
    13801 ggtttgacgg cttctatgcc cgccatcatg gaggcgtggg ttacgtcgat caggcgttgg
    13861 gcgatggggg agactttgcc gacggtaaac atacggctgg agtcgccgtg gaagccgtct
    13921 tttttgatgg tgaggtcgat gttgataatg tcgccttctt tgagcggctt gtcgtcggga
    13981 atgccgtggc agatgacgtg gttgacggag gtgcagcagg atttggggta gggcgggttg
    14041 ccgtagtgca ggggggcggg atagccgcct tggacgttga cgtggtagtc gtaaacgagt
    14101 ttgtcgattt cgtcggtggt tacgccgggt ttgacgaatt gtccgatgta gtcgagggct
    14161 tcggcgacga gtttgcccag ctcgcgcatt ttttcgattt cttcgggggt tttgatgatg
    14221 atgccgttca taatgcttcc ttggattgcg ggtgtgcctg ccgtgccggg tcagaagagg
    14281 ttgtcgagaa tttctttatg gggtttgggc ggcgtgtttt tcggggtgtc cggtttggta
    14341 tggttttctt tgggcgtttc tttttctttg ggtgtttctt tgggctgcgc ctgtttttcg
    14401 ggtgtttctt ttcggggttc gggacggggg acgtttgtgg gcagtacggg ggtttctttg
    14461 agtgtgtttt cagcttgtgt tccggcgtcg tccggctgcc tgtcggtttg agctgtgtcg
    14521 gcaggttgcg gtttgccccg gtttttcttg ggtgcggcag gggacggcag tccgctgccg
    14581 ctttcgtctg tgccgtccgg ctgtgcgggt tcggatgagg cggcgtggtg gtgttcgggt
    14641 tgggcggcat cttgtccgac tacgccgttt ggcagccaga attcggtttc gcgggggctg
    14701 tcggtgtgtt gcggttcggt ttggagggtt ttgccgccgt tcgggttgaa tgtgctgacg
    14761 atgcctattg ccaatgcgcc gatggcggtg atacagatga gcaatacgac gcgtatcagg
    14821 agtttggggg tcagcctgaa gggtttgttc ggtttttttg ccattttgat tgtgcttttg
    14881 gggtgtcggg caatgccgtc tgaaggcggt tcagacggca ttgccgagtc agcgttggac
    14941 gtagttttgg tagagggtga tgactttttg tacgccgacg gtggtgctga ctttttgggt
    15001 aatctgcgcc tgttcttcgg gggtgaggat gcccataacg taggttacgt tgccgtaggt
    15061 aacgattttg acgcgcgcct gtgtggcggg gctgatgccc aacagcgtgg cgcggacttt
    15121 ggatgtgttc caagtgtcgc cggcgatgtc gccggcagtg cgcggcaggg aggcgacggt
    15181 aatgtagttg tatacgcctt cggcggcctg ttcggaacgt gcaatctgac cgacgaactg
    15241 tttctcgcct tcggtggcga cttgtccgag cagcagcagg tggcggttgt agccgacaac
    15301 ggagatttgg ggcgtgtagc ctttggtttg gttgttttgg cgcagatagg agcgggcggt
    15361 ggtttcgata cgcaacgcca taacgttgtc gtcggtttgc gcgccggtgg ttcggcggtc
    15421 gacggcggat ttcgcgccga cggcggcgct tccgattact gcgctgacgc agccgctaag
    15481 ggcaaggctg aaaatggcgg caatcagggt gcggacggtg tgcggtttgg gtttcatcgg
    15541 gtgcttcctt tcttgggcgt ttcagacggc attgctttgc gccatgccgt ctgaaaaggg
    15601 ttacattcct tccagcagta cggagtcgat acagtcgcac atggcgtgta tcagcaggat
    15661 gtggttttcc tgaatacggg cagttcgagg atagggaacg ttgagtaaaa cgtcggtgtc
    15721 tttgagcatg gcggcgattt tgccgccgtc gcggccggtc agggcgatga cgtgcatatc
    15781 gcgttcgtgt gcggctttga cggcttcgat gacgttggcg gaattgccgg aagtggaaat
    15841 gccgaccaag acgtcgcctg cacgtccgag ggcgcgtacc tgtttgctga agacgtggtt
    15901 gaagccgtag tcgttaccga tggcggtcag cgcggaggtg tctgtggtca gggcgacggc
    15961 ggcgagttcc atgcgctctt tttcaaagcg tccggtcatt tcggcggcga agtgctgggc
    16021 atcggcggcc gaaccgccgt tgccgcacgc cagaatcttg ccgtcgttca tcaggcattg
    16081 cagcatcagc tcggcagcct gtacggtcgg ctcgaccagt actttttcgg cttcctgttt
    16141 ggcacggatg ctttcggcaa aatgggcggc aacgcgttct tgtaatgtcg tcatatcgtc
    16201 aacctgtaat attctgtatc cactcgggcg ggcggtttcc ttcaatcagt accgcatcga
    16261 ggcggcacgg tacgtttgtc agcctgttct gttgcagata atactctaca cttcgttgca
    16321 gtttcaataa tttggatggg gaaatgctgt atgcgacacc gccgaattgc cgatttttgc
    16381 ggtattttac ttcaacaaac agaatcatac cgccgttttt gacaatcagg tcgatttcgc
    16441 cgtaggcgca gtgccagttg cgggcaagca gtgtgcagcc ttgggattgg aggaaggcaa
    16501 gcgcggcatc ttcccctgcc tcgccctgtt tgtggtttag gcgcataatc ggtttaacct
    16561 ttataatgtt tgtttttcag acggcattct tatgtttcag aaacatttgc agaaagcctc
    16621 cgacagcgtc gtcggaggga cattatacgt ggttgccacg cccatcggca atttggcgga
    16681 cattaccctg cgcgctttgg cggtattgca aaaggcggac atcatctgtg ccgaagatac
    16741 gcgcgttacc gcgcagcttt tgagcgcgta cggcattcag ggcaaacttg tcagcgtgcg
    16801 cgaacacaac gaacggcaga tggcggacaa gattgtcggc tatctttcag acggcatggt
    16861 tgtggcacag gtttccgatg cgggtacgcc ggccgtgtgc gacccgggtg ccaaactcgc
    16921 cggccgcgtg cgtgaggccg ggtttaaagt cgttcccgtc gtgggcgcaa gcgcggtgat
    16981 ggcggctttg agcgtggccg gtgtggaagg atccgatttt tatttcaacg gttttgtacc
    17041 gccgaaatcg ggagaacgca ggaaactgtt tgccaaatgg gtgcgggcgg catttcctat
    17101 cgtcatgttt gaaacgccac accgaatcgg ggcaacgctt gccgatatgg tggaattgtt
    17161 ctccgaacgc cgattaatgc tggcgcgcga aattacgaaa acgtttgaaa cgttcttaag
    17221 cggcacggtt ggggaaattc agacggcatt gtctgccgac ggcaaccaat cgcgcggtga
    17281 gatggtgttg gtgctttatc cggcgcagga tgaaaaacac gaaggcttgt ccgagtccgc
    17341 gcaaaacatc atgaaaatcc tcacagccga gctgccgacc aaacaggcgg cggagcttgc
    17401 cgccaaaatc acgggcgagg gaaaaaaagc tttgtacaat ctggcactgt cttggaaaaa
    17461 caaatagtgc cggacgagcc ggcaccatag tgtgaacgga atcagcggac acgacccgtc
    17521 atgcggcaca gaacatctct accctgaaca cggtgggcga cgaccatggc gacgtgtccg
    17581 gcgacggcgg caaacagcag caagcccaaa ttgccgtgga acgtgttgcc caagtttgcc
    17641 atccactcga ttttttccgg cgaacccttg catcacttca acgccgaaca ctttcaacgg
    17701 gccgcggccg ctgccgtatt ggcggatcat gccgataacg ggaacagcaa gcatgagcag
    17761 atacagaatg ccgtggcctg ccgccgcagc cttgctgtcg ctttgcggac gcttggcgcg
    17821 gttggcaacc gcccacacga tgcgcaatgt aatcaccgtc agcgtaagga aaccgaaaga
    17881 tttgtgcagg ccgaacaggc tgcccaccca ttccgcctct tcgtatagtg gattaacaaa
    17941 aaccagtacg gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc
    18001 ttgtcctgat ttttgttaat ccactataaa tcgtccacag gacaatggtg gtcagaatgc
    18061 cgatgaagcc ggcaacggtc agccagtgca gcgcgcgtgt caacgggtcg taaacggcag
    18121 gttggtttgt gttcataaaa aactt
//
LOCUS       DAWFHY010000040        17835 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.040, whole genome shotgun sequence.
ACCESSION   DAWFHY010000040 DAWFHY010000000
VERSION     DAWFHY010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17835
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.040"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(379..4548)
                     /locus_tag="WGQ68_001185"
     CDS             complement(379..4548)
                     /locus_tag="WGQ68_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEZ0714983.1"
                     /translation="MTDTAPTDTDPTENGTRKMPSEHRPTPPAKKRRPLLKLSAALLS
                     VLILAVCFLGWLAGTEAGLRFGLYQIPSWFGVNISSQNLKGTLLDGFDGDNWSIETEG
                     ADLKISRFRFAWKPSELMHRSLHITEISAGDIAIVTKPTPPKEERPPLSLPDSIDLPA
                     AVYLDRFETGKISMGKAFDKQTVYLERLDASYRYDRKGHRLDLKAADTPWSSSSGAAS
                     VGLKKPFALDTAIYTKGGLEGKTIHSTARLSGSLKDVRAELAIDGGNIRLSGKSVIHP
                     FAESLDKTLEEVLVKGFNINPAAFVPSLPDAGLNFDLTAIPSFSDGIALEGSLDLENT
                     KAGFADRNGIPVRQVLGSFVIRQDGTVHIGNTSAALLGRGGIRLSGKIDTGKDILDLN
                     IGINSVGAEDVLQTAFKGRLDGSIGIGGTTASPKISWQLGIGTARTDGSLAIASDPAN
                     GQRKLVLDTVNIAAGQGSLTAQGYLELFKDRLLKLDIRSRAFDPSRIDPQLPAGNING
                     SINLAGELAKEKFTGKMRFLPGTFNGVPIAGSADIVYESRHLPRAAVDLRLGRNIIKT
                     DGGFGKKGDRLNLNITAPDLSRFGFGLAGSLNVRGHLSGDLDGGIRTFETDLSGAARN
                     LHIGKAADIRSLDFTLKGSPDTSRPIRADIKGSRLSLSGGAAVVDTADLMLDGTGVQH
                     RIRTHAAMTLDGKPFKFDLDASGGINRELTRWKGSIGILDIGGAFNLKLQNRMTLEAG
                     AERVAASAANWQAMGGSLNLQHFSWDKKTGISAKGGAHGLHIAELHNFFKPPFEHNLV
                     LNGDWDVAYGRNARGYLNISRQSGDAVLPGGQALGLNAFSLKTRFQNDRIGILLDGGA
                     RFGRINADLGIANAFGGNMANAPLGGRITASLPDLGALKPFLPAAAQNITGSLNAAAQ
                     IGGRVGSPSVNAAVNGSSNYGKINGNITVGQSRSFDTAPLGGRLNLTVADAEVFRNFL
                     PVGQTVKGSLNAAVTLGGSIADPHLGGSINGDKLYYRNQTQGIILDNGSLRSHIAGRK
                     WVIDSLKFRHEGTAELSGTVGMENSGPDVDIGAVFDKYRILSRPNRRLTVSGNTRLRY
                     SPQKGISVTGMIKTDQGLFGSQKSSMPSVGDDVVVLGEVKKEAAAPLPVNMNLTLDLN
                     DGIRFAGYGADVTIGGKLTLTAQSGGSVRGVGTVRVIKGRYKAYGQDLDITKGTVSFV
                     GPLNDPNLNIRAERRLSPVGAGVEILGSLNSPRITLTANEPMSEKDKLSWLILNRAGS
                     GSSGDNAALSAAAGALLAGQINDRIGLVDDLGFTSKRSRNAQTGELNPAEQVLTVGKQ
                     LTGKLYIGYEYSISSAEQSVKLIYRLTRAIQAVARIGSRSSGGELTYTIRFDRFSGSD
                     KKDSAGNSKGK"
     gene            complement(4603..6450)
                     /locus_tag="WGQ68_001186"
     CDS             complement(4603..6450)
                     /locus_tag="WGQ68_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor"
                     /protein_id="HEZ0714984.1"
                     /translation="MHDTRTMMIKPTALLLPALFFFPHAYAPAADLSENKAAGFALFK
                     NKSPDTESVKLKPKFPVRIDTQDSEIKDMVEEHLPLITQQQEEVLDKEQTGFLAEEAS
                     DNVKTMLRSKGYFSSKVSLTEKDGAYTVHITPGPRTKIANVGVAILGDILSDGNLAEY
                     YRNALENWQQPVGSDFDQDSWENSKTSVLGAVTRKAYPLAKLGNTRAAVNPDTATADL
                     NVVVDSGRPIAFGDFEITGTQRYPEQIVSGLARFQPGTPYDLDLLLDFQQALEQNGHY
                     SGASVQADFDRLQGDRVPVKVSVTEVKRHKLETGIRLDSEYGLGGKIAYDYYNLFNKG
                     YIGSVVWDMDKYETTLAAGISQPRNYRGNYWTSNVSYNRSTTQNLEKRAFSGGIWYVR
                     DRAGIDARLGAEFLAEGRKIPGSDIDLGNSHATMLTASWKRQLLNNVLHPENGHYLDG
                     KIGTTLGAFLSSTALIRTSARAGYFFTPENKKLGTFIIRGQAGYTVARDNANVPSGLM
                     FRSGGASSVRGYELDSIGLAGPNGSVLPERALLVGSLEYQLPFTRTLSGAVFHDMGDA
                     AANFKRMKLKHGSGLGVRWFSPLAPFSFDIAYGHSDKKIRWHISLGTRF"
     gene            6980..8209
                     /gene="sstT"
                     /locus_tag="WGQ68_001187"
     CDS             6980..8209
                     /gene="sstT"
                     /locus_tag="WGQ68_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEZ0714985.1"
                     /translation="MAFGKSLFHAIGRVSLVRQIAAGLALGIVIGSVSPQLGLAAGLF
                     GSLFVGALKAVAPVLVFILVAATIAQHQKGNKAHIRPIIILYLIGTFSAALTAVIAGM
                     VFPTHIVLAGAGDVSAAPPSGIVEVLKSLLMNLVANPINAIANANYIGILAWALVLGA
                     ALRNHGSDVTRQVVADLAEAVSTVVKWIIRFAPLGIFGLVSSTIAETGFGALAGYAKL
                     LAVLLGCMAFIALVVNPAIVWWKIRRNPYPLVFTCLRESGVYAFFTRSSAANIPVNMA
                     LAKKLGLHEDTYSISIPLGATVNMGGAAITITVLAMAAAHTQGIQVDFATALLLSLVA
                     TVSACGASGVAGGSLLLIPLACSLFGISNDVAMQVVAVGFIIGVIQDSAETALNSSTD
                     VLFTAAADLGRQRNRAE"
     gene            8465..9925
                     /locus_tag="WGQ68_001188"
     CDS             8465..9925
                     /locus_tag="WGQ68_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0714986.1"
                     /translation="MFKRSVIAMACIFALSACGGGGGGSPDVKSADTLSKPAAPVVSE
                     KETEAKEDAPQAGSQGQGAPSAQGSQDMAAVSEENTGNGGAATADNPKNEDEGAQDDM
                     PQKAAGTDSSTPNHTPDPNMPAGNMENQATDAGESEQPANQPDMANTADGMQGDDPSA
                     GGENAGNTAAQGANQAGNNQAAGSSDSTPASNPATTNSGGDFGRTNVANGIKLDGPSQ
                     NITLTHCKDKVCGSDFLDEEAPPKSEFESLDDSGRINKYKKDGQDKFTNLVATEVKAN
                     GTNKYVIIYKDKSTSSARVRRSARSRRSLPAEMPLIPVNQADTLIVDGEAVSLTGHSG
                     NIFAPEGNYRYLTYGAEKLPGGSYALRVQGEPAKGEMLAGTAVYNGEVLHFHTENGRP
                     YPTRGRFAAKVDFGSKSVDGIIDSGDDLHMGTQKFKAAIDGNGFKGTWTENGGGDVSG
                     KFYGPAGEEVAGKYSYRPTDAEKGGFGVFAGKKEQD"
     gene            complement(10000..10215)
                     /locus_tag="WGQ68_001189"
     CDS             complement(10000..10215)
                     /locus_tag="WGQ68_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714987.1"
                     /translation="MFKLGVYTCLGLFAGWVLLLIVQLWFSFLEAELFFKITLTMAGL
                     FVIILAALLVCGQYFSEKKMKDDGFIN"
     gene            complement(10208..10423)
                     /locus_tag="WGQ68_001190"
                     /pseudo
     CDS             complement(10208..10423)
                     /locus_tag="WGQ68_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242091.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
     gene            complement(10498..11841)
                     /gene="argG"
                     /locus_tag="WGQ68_001191"
     CDS             complement(10498..11841)
                     /gene="argG"
                     /locus_tag="WGQ68_001191"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEZ0714988.1"
                     /translation="MSQNHTILQSLPVGQKVGIAFSGGLDTSAALLWMKLKGALPYAY
                     TANLGQPDEDDYNAIPKKAMEYGAENARLIDCRAQLAHEGIAAIQCGAFHVSTGGIAY
                     FNTTPLGRAVTGTMLVSAMKEDDVNIWGDGSTYKGNDIERFYRYGLLTNPALKIYKPW
                     LDQQFIDELGGRHEMSEFLIANGFNYKMSVEKAYSTDSNMLGATHEAKDLEFLNSGIK
                     IVKPIMGVAFWDENVEIKPEEVSVRFEEGVPVALNGKEYADPVELFLEANRIGGRHGL
                     GMSDQIENRIIEAKSRGIYEAPGMALFHIAYERLITGIHNEDTIEQYRINGLRLGRLL
                     YQGRWFDSQALMLRETAQRWVAKAVTGEVTLELRRGNDYSILNTESPNLTYQPERLSM
                     EKVEGAAFTPLDRIGQLTMRNLDITDTRAKLGIYSQSGLLALGEGSVLPQLGNKK"
     gene            12168..12308
                     /locus_tag="WGQ68_001192"
     CDS             12168..12308
                     /locus_tag="WGQ68_001192"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714989.1"
                     /translation="MQTAALKQTLPEKRKLDPLPNPLPWEREQITAPSTFSDGIGKET
                     HL"
     gene            12305..13105
                     /locus_tag="WGQ68_001193"
     CDS             12305..13105
                     /locus_tag="WGQ68_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEZ0714990.1"
                     /translation="MNAFNLTIIGDEILHGSRQDKHFAFFKSLLESKGLKLNQVQYLP
                     DEPDLLVRQLRRSFSDGIPTFVTGGIGATPDDHTRQAAADALELPVVRHPEAAGFIEG
                     ITRKRGEPLDSPEHAQRLKMADFPEGAELVPNPFNNIAGFSIREHYFFPGFPVMAHPM
                     AEWVLETYYADRFNQTERGSRSVYVFEQPESRITPLMEHIEQTYPGVRSYSLPSVGWT
                     HSDGTQVKPHIEFGIKAEGEAVNLLDAAWAEVLHSLDGLGAELKNRVN"
     gene            13145..14131
                     /locus_tag="WGQ68_001194"
     CDS             13145..14131
                     /locus_tag="WGQ68_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HEZ0714991.1"
                     /translation="MQYRIRRENEAPEAKNAGETLWERDIMREVLLSAYQDRRRERMW
                     KNIWRAVSTLILVALIAGIFRKDEAALQLAGNTPHTAVVNLYGEIGNGVEDQVKKLKD
                     GMEAAYKNPQAKAIVIRANSPGGSPVVSNTAFEEIRRLKAQHPGIPVYLVAEDMCASG
                     CYYIAAAADKIYADPSSIVGSIGVIGSSFDATGLMEKIGVKRRVKIAGSNKGMGDPFS
                     PETPEQSKIWEEMLTGIHGEFIKAVKTGRGGRLKFRQYPDVFSGRVYTGADALKVGLV
                     DGLGNIYSVARDVVKAPDVVDYTPKDDFGRILGRRFGAELKASVREALQAVR"
     gene            14787..15049
                     /locus_tag="WGQ68_001195"
                     /pseudo
     CDS             14787..15049
                     /locus_tag="WGQ68_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021111752.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(15087..15461)
                     /locus_tag="WGQ68_001196"
                     /pseudo
     CDS             complement(15087..15461)
                     /locus_tag="WGQ68_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709227.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15634..15990)
                     /locus_tag="WGQ68_001197"
     CDS             complement(15634..15990)
                     /locus_tag="WGQ68_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mimic protein"
                     /protein_id="HEZ0714992.1"
                     /translation="MNEHNLLIFCLKDNVSISEYTEMIDWAYKNIQSETVVEITESQI
                     IEYQNRGLWRLVSEITDNWLFGPSEGDWLIDKESILAVKEKLQNSDFSTEPLVKNIIH
                     VLEYAIKNKKTVIFHF"
     gene            complement(16130..16474)
                     /locus_tag="WGQ68_001198"
     CDS             complement(16130..16474)
                     /locus_tag="WGQ68_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714993.1"
                     /translation="MNLENYENILIKLLFYHNNLVNEYSYFIENKNLFKIVSRTKTSK
                     GFFFTIEKPLNFLSKKTYFEFNFKYLHSGKERFGSFMCWINTNLMEFEGVFFNDLLPD
                     NMIINNFFEIND"
     gene            complement(16633..17034)
                     /locus_tag="WGQ68_001199"
     CDS             complement(16633..17034)
                     /locus_tag="WGQ68_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714994.1"
                     /translation="MKLKSLDFPTGYFYFDNAAINSDKVEVIAVGYRNTDKTIKIFIE
                     DVIHFRVVDESYFIDTFMDLISEDADRALLHENGGQSFFELLDECYAEWILKESYFPL
                     NREFFKYYIFMFEQTFIEIIGSSATYSIIEG"
     gene            complement(17038..17778)
                     /locus_tag="WGQ68_001200"
                     /pseudo
     CDS             complement(17038..17778)
                     /locus_tag="WGQ68_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713004.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
BASE COUNT         4104 a         4376 c         4861 g         4494 t
ORIGIN      
        1 atgtaggttt tcttaaccct gcgtcctaga ttcccgcttt tgcgggaatg acggaaagtg
       61 gcgggaatga cggttcgggc attccttaaa ttacctgtgt atcgctgtaa atcttagaga
      121 tggcggaata tagcggatta acaaaaaccg gtacggcgtt gcctcgcctt ggctcaaaga
      181 gaacgattct ctaaggtgct caagcaccga gtgaatcggt tccgtactat ttgtactgtc
      241 tgcggcttcg tcgccttatc ctgatttttg ttaattcact atatcgattc tgttcccttg
      301 caggcggacg gcggcggcgg acggtggaaa agcaggtttt caaatgtccg gtttggcggc
      361 gcgccgtctg aaaaccgctt attttccttt gctgtttccg gcggagtctt ttttgtccga
      421 accggagaag cggtcgaaac gtatggtgta tgtcagctcg ccgcccgacg aacggctgcc
      481 gatacgggca accgcctgta tggcgcgggt cagccggtaa atcagtttga cggactgttc
      541 cgcgctggag atgctgtatt cgtagccgat gtagagtttg ccggtcagtt gtttgccgac
      601 ggtcagcacc tgttcggcgg ggttgagttc gccggtttgc gcgttgcggc tgcgcttgct
      661 ggtaaagccc aaatcatcca ccagcccgat gcggtcgttg atttgcccgg caagcagcgc
      721 gcctgcggct gcggacaggg cggcattgtc gccgctgctg ccgctgccgg cgcggttgag
      781 gatgagccaa gagagcttgt ctttttcact catcggttcg tttgccgtca gcgtaatgcg
      841 cgggctgttg aggctgccca atatttccac gcccgcaccg acgggggaaa ggcggcgttc
      901 ggcgcggatg ttgaggttgg ggtcgttgag cgggccgaca aaggagaccg tgcctttggt
      961 aatgtccaaa tcctgcccgt atgccttata acgccctttg atgacgcgga ccgtgcccac
     1021 gccccgcacg cttccgcccg attgggcggt cagggtcagt ttgccgccta tggtaacgtc
     1081 cgcgccgtag ccggcgaagc ggatgccgtc attgaggtct aaagtcaggt tcatattgac
     1141 ggggagcggt gccgccgcct cttttttgac ttcgcctaat acgacgacat cgtcgccgac
     1201 ggacggcatc gaggattttt gcgaaccgaa cagcccctga tccgttttaa tcatcccggt
     1261 aacggatatg cctttttgcg gcgaatagcg caggcgggtg ttgccggaaa ccgtcaggcg
     1321 gcggttgggg cgggacagga tgcggtattt gtcgaacacc gcgccgatat cgacatcggg
     1381 tccgctgttt tccataccga ccgtaccgga gagttccgcc gtcccttcgt gacggaattt
     1441 caggctgtcg attacccatt tcctgcccgc gatatgcgaa cgcagcgagc cgttgtccaa
     1501 gatgatgcct tgggtttggt tgcggtaata gagtttgtcg ccgttgatgc tgccgcccaa
     1561 gtgcggatcg gcgatgctgc cgccgagggt tacggcggca ttcaggctgc ctttgacggt
     1621 ttgtccgacc ggtaggaagt tgcggaatac ttcggcatcg gcaacggtca ggttgagcct
     1681 gccgcccaaa ggcgcggtat cgaaagagcg gctttgcccg acggtgatgt tgccgttgat
     1741 tttcccgtag ttgctgctgc cgttgacggc ggcattgacg gacggagagc ctacccgtcc
     1801 gccgatttgc gcggcggcat tcaggctgcc ggtaatgttt tgcgcggcgg cgggcagaaa
     1861 gggcttcaat gcgcccaagt cgggaaggga ggcggtaatc ctgccgccga gcggtgcatt
     1921 tgccatattg ccgccgaagg cgttggcgat gcccaaatcg gcgttaatcc gcccgaaacg
     1981 cgcgccgccg tcaagcagga ttccgatacg gtcgttttga aagcgcgttt tcagggaaaa
     2041 tgcgttcaaa cccaaagcct gcccgccggg caatacggca tcgccgcttt gccggctgat
     2101 attgaggtag ccgcgcgcgt tgcgcccgta ggcgacatcc cagtcgccgt ttaaaaccag
     2161 attgtgttcg aagggcggtt tgaagaaatt gtgcaactcg gcgatatgca gaccgtgtgc
     2221 gccgcctttt gccgatatgc cggttttttt atcccaagaa aagtgttgca ggttgaggct
     2281 gccgcccatt gcctgccaat ttgccgcact tgccgccacg cgttccgcac cggcttcgag
     2341 cgtcatacgg ttttgcagct tgaggttgaa tgcgccgccg atgtcgagga tgccgatgct
     2401 gcctttccat cgggtaagtt ccctgttgat gccgcctgaa gcgtccaaat cgaatttgaa
     2461 cggtttgcca tccagcgtca tggcggcgtg tgtgcggatg cggtgctgca cgcccgtgcc
     2521 gtccagcatc aggtcggcgg tatcgacaac cgccgctccg cccgacagcg aaaggcggct
     2581 gcctttgatg tcggcgcgta tcgggcggct tgtgtcgggc gaacctttga gcgtgaaatc
     2641 gagcgaacgg atgtctgccg ccttgccgat gtgcaggttg cgcgccgcgc cggaaaggtc
     2701 ggtttcaaag gttcggatgc cgccgtccaa atcgccggaa aggtgtccgc gtacatttaa
     2761 agaccccgcg agtccgaaac cgaaacggga taaatcgggt gcggtgatat tgaggttaag
     2821 ccggtcgcct tttttgccga agccgccgtc tgttttaata atgttccgcc ccagccgcaa
     2881 atcgacggcg gcacgcggaa ggtggcggga ctcgtaaaca atgtcggcac tgccggcaat
     2941 cggtacgccg ttgaacgtgc cgggtaaaaa ccgcattttg cctgtgaatt tctcttttgc
     3001 cagttcgccg gcaaggttta ttgagccgtt gatattgcct gccggaagtt gcggatcgat
     3061 gcgcgaaggg tcgaatgcgc gggaacggat gtccagcttg agcaggcggt ctttaaacag
     3121 ctcgagatag ccttgcgcgg tcaggctgcc ttgcccggcg gcgatgttga cggtgtcgag
     3181 caccagtttc cgctgtccgt ttgcggggtc gcttgcaatg gcgaggctgc cgtccgtgcg
     3241 cgccgtgccg atgccgagtt gccaagagat tttgggcgag gcggtcgtgc caccgatgcc
     3301 gatgctgccg tccaacctgc ctttgaacgc ggtttgcagt acgtcttccg cgccgacgga
     3361 gttgatgcct atatttaaat cgaggatgtc ttttccggtg tcgattttgc ccgacagcct
     3421 gatgccgccc cgtccgagca gggcggcgga cgtattgccg atatgcaccg tgccgtcctg
     3481 ccggatgaca aagctgccta aaacctgacg gacggggatg ccgttgcggt cggcaaaacc
     3541 ggctttggtg ttttccaaat cgagcgagcc ttccagcgcg atgccgtctg aaaacgacgg
     3601 gatggcggtc aggtcgaaat tcagcccggc atcgggcagg gaaggtacga aggcggccgg
     3661 attgatgttg aaccctttga ccagtacttc ttccaatgtt ttatccaatg attcggcaaa
     3721 cgggtggatg acggattttc ccgagaggcg gatattgccg ccgtcgatcg ccagttcggc
     3781 gcgcacatcc ttcaggctgc cgctcagccg agccgtactg tgtatggttt tgccttcgag
     3841 tccgcctttg gtgtaaatgg cggtatcgag ggcaaacggt tttttcaagc cgaccgaggc
     3901 cgcccccgac gaactgctcc acggcgtgtc ggcggccttc aggtcaaggc ggtgtccttt
     3961 gcggtcgtaa cggtatgaag catccagccg ttcgagatag acggtttgtt tgtcaaaggc
     4021 tttgcccatg ctgattttgc ccgtctcgaa gcggtcgaga tagacggcgg caggcaggtc
     4081 tatgctgtcg ggaaggctga gcggcgggcg ttcttcttta ggcggagtcg gtttggtaac
     4141 gatggcgatg tcgccggcgg aaatttcggt aatgtgcagg ctgcggtgca tcagttcgga
     4201 cggtttccac gcgaagcgga agcggctgat tttaaggtct gccccctcgg tttctatcga
     4261 ccaattgtcg ccgtcgaagc cgtcgagcag cgtgcctttg aggttttggg aggaaatgtt
     4321 tacgccgaac caagacggga tttggtacag cccgaagcgc aaacccgctt ccgtgccggc
     4381 gagccagccg aggaaacata ctgccaaaat cagaacagac agcagtgccg ccgacagctt
     4441 cagcagcggg cggcgttttt ttgccggcgg ggtagggcgg tgttcagacg gcattttgcg
     4501 cgtgccgttt tcggtcggat cggtatctgt cggtgcggta tcggtcataa tgtctgtttc
     4561 aaaatggttt gctgcaatgc cgtctgaagc ggccgtatcg gtttagaagc gcgttcccaa
     4621 gctgatgtgc cagcggattt tcttgtcgct gtgcccgtag gcgatgtcga aggaaaacgg
     4681 cgcgagcggg ctgaaccagc gcacgcccag tcccgaaccg tgtttcagct tcatacgttt
     4741 gaaattggcg gcggcgtcgc ccatatcgtg gaacaccgcg ccggaaaggg tgcgcgtaaa
     4801 cggcagttgg tattccaggc tgcccaccaa gagggcgcgt tcgggcagga ccgatccgtt
     4861 cgggccggca agcccgatgc tgtcgagttc gtaaccgcgc acggaagacg cgccgccgct
     4921 gcggaacatc agccctgaag gaacgttggc attgtcgcgg gcaacggtgt aacccgcttg
     4981 tccgcgtatg atgaacgtgc cgagtttttt gttttcgggc gtgaagaaat aacctgcgcg
     5041 ggcagaggtg cggatcagcg cggtggagga caggaatgcg cccaaagtcg taccgatttt
     5101 gccgtcgagg taatggccgt tttcgggatg cagcacgttg ttgagcagct ggcgtttcca
     5161 agaggcggtc agcatcgtgg cgtggctgtt gcccaaatcg atatccgagc cggggatttt
     5221 ccggccttct gcgagaaact ccgcccccag cctggcatcg atgcccgcgc ggtcgcgcac
     5281 ataccagatg ccgccggaga aggcgcgttt ttcgaggttt tgggtggtcg aacggttgta
     5341 ggaaacgttg cttgtccagt agttgccccg atagttgcgc ggctggctga tgccggcggc
     5401 aagcgtggtt tcgtatttgt ccatatccca gacgaccgaa ccgatatagc ctttgttgaa
     5461 gaggttgtaa tagtcgtagg cgattttgcc gcccaaaccg tattccgaat cgaggcggat
     5521 gccggtttcg agcttgtggc gtttgacctc ggttacgctg actttgacgg ggacgcggtc
     5581 gccttggagg cggtcgaagt cggcttgtac ggacgcgccg gaataatgcc cgttttgttc
     5641 gagcgcctgt tggaagtcga gcagcaggtc gaggtcgtag ggcgtgcccg gttggaagcg
     5701 cgccaggccg gagacgattt gttcggggta acgctgcgtg ccggtaattt caaagtcgcc
     5761 gaaggcgatg gggcggccgc tgtccacgac gacgttcaaa tcggcggtgg cggtatcggg
     5821 gttgacggcc gcccgggtgt tgccgagctt ggcaagcggg taggctttgc gcgttaccgc
     5881 gccgaggacg gaagttttgc tgttttccca actgtcctga tcgaaatcac tgcctaccgg
     5941 ctgctgccag ttttccagcg cgttgcggta gtattcggcg aggttgccgt ctgaaaggat
     6001 gtcgccgagg atggcgacgc cgacgttggc gattttggtg cgcgggcccg gtgtgatgtg
     6061 taccgtataa gctccgtctt tttccgtcag gctgactttg ctgctgaaat agcctttgct
     6121 gcggagcatc gttttaacgt tgtccgatgc ttcttcggcg aggaagcccg tctgttcttt
     6181 gtccaatact tcttcctgct gctgcgtgat gagcggcagg tgttcttcga ccatatcttt
     6241 gatttcactg tcctgcgtgt cgatgcggac ggggaatttg ggttttaatt tgactgattc
     6301 ggtgtcgggg cttttgtttt tgaacaatgc gaaacccgcc gccttgtttt cggaaaggtc
     6361 ggcggcaggc gcgtatgcgt gcggaaagaa aaataaagcc ggcaggagca gggcggtcgg
     6421 tttaatcatc atggtacggg tatcgtgcat cggtgtggcg gatttcgatt ctaacactgt
     6481 tcggacgggg cgggacgctt cagacggcat actgccgccg gtatttcgga ggttgcggcc
     6541 cgctggaaat gtatctgctt gttttaaggt attttgtttc taatccgcat atggaaaaca
     6601 ggctgcgttt cggaatagtg tcggaaaata aacgggatta aataaattta ttttcgttca
     6661 ataaattaaa ccgtcgcgcc gaatgataaa gcaaagcctg tccggcactg catagggcaa
     6721 atcaacgcaa ccttcatagt ggattaacaa aaaccagtac ggcgttggct cgccttagct
     6781 caaagagaac gattctttaa ggtgctgaag caccaagtga atcggttccg tactatttgt
     6841 actgtctgcg gcttcgtcgc cttgtcatga tttttgttaa tccactataa aacacagccg
     6901 aaaaataaaa aaaacacaga gaatgagttt tcttttaaaa tacaacattt tttaacatat
     6961 cacaacataa ggaagaatta tggcgttcgg caaatcctta tttcatgcga tcggccgcgt
     7021 cagcctggtc aggcagattg ccgccggttt ggcgttgggc atcgtaatcg gttcggtttc
     7081 cccgcaactg ggcttggcgg caggcttgtt cggcagcctg tttgtcggcg cgctcaaagc
     7141 ggtcgcgccg gttttggtat ttattttggt ggcggccaca atcgcgcagc accaaaaagg
     7201 caacaaggcg catatcaggc cgattatcat cctttacctc atcggtacgt tttccgcagc
     7261 cctgaccgcc gtcatcgccg gtatggtttt cccgacgcac attgttttgg cgggcgcggg
     7321 cgatgtgtcc gccgcgccgc cttccggcat tgtggaagta ttgaaatcgc tgctgatgaa
     7381 cctggtcgcc aacccgatta acgcgattgc caatgccaac tatatcggca ttttggcttg
     7441 ggctttggtt ttgggcgcgg cgttgcggaa tcacggttcg gacgttacgc ggcaggtcgt
     7501 tgccgatttg gcggaagcgg tttccaccgt cgtgaaatgg attatccgtt ttgccccttt
     7561 gggcattttc gggctggtgt cgtccacaat cgcggagacg ggtttcggcg cgctggcggg
     7621 ttatgcgaag ctgcttgcgg tactgttggg ctgtatggcg tttatcgcgc tggtggtcaa
     7681 tcccgccatc gtgtggtgga aaatccgccg caacccttat ccgctggtgt ttacctgcct
     7741 gcgcgaaagc ggcgtgtatg ccttctttac ccgttcttcc gccgccaata tccccgtgaa
     7801 tatggctttg gcgaaaaaac tggggctgca cgaagacact tattcgattt ccatcccgtt
     7861 gggggcgacc gtcaatatgg gcggcgcggc gattacgatt accgttttgg ctatggcggc
     7921 ggcgcacact caaggcatac aggttgattt tgccaccgcg ctgctgttga gcctggtggc
     7981 gacggtcagc gcgtgcggcg cgtccggtgt ggcaggcggt tcgctgctgc tgattccgct
     8041 ggcgtgcagc ctgttcggca tcagcaacga tgtcgctatg caggtggtcg ccgtcggttt
     8101 cattatcggc gtgattcagg attcggcgga aactgcgctg aactcttcga ccgacgtttt
     8161 gtttaccgct gccgccgatt tgggccgaca gaggaaccgg gcggaataaa ccaagctatg
     8221 ccgtctgaag cccgtttggc gttcagacgg catattttta caattttata ggcatacggc
     8281 ggaaacaata tttattgaat atgtttcaac acacagggcg gcacataaag caccgcccta
     8341 tgtgttgccc tgatttggaa gggttgcgcc cttcccaaat aaagtctgat tccaccgccc
     8401 cgaaggacgg atgtccaagt ggcggggttt aaaccaaaaa ggaaatacga tgaaggagat
     8461 gatgatgttt aaacgcagcg taatcgcaat ggcttgtatt tttgcccttt cagcctgcgg
     8521 gggcggcggt ggcggatcgc ccgatgtcaa gtcggcggac acgctgtcaa aacctgccgc
     8581 ccctgttgtt tctgaaaaag agacagaggc aaaggaagat gcgccacagg caggttctca
     8641 aggacagggc gcgccatccg cacaaggcag tcaagatatg gcggcggttt cggaagaaaa
     8701 tacaggcaat ggcggtgcgg caacagcaga taatcccaaa aatgaagacg agggggcgca
     8761 agatgatatg ccgcaaaaag ccgccggtac agatagttcg acaccgaatc acaccccgga
     8821 tccgaatatg cctgccggaa atatggaaaa tcaagcaacg gatgccgggg aatcggagca
     8881 gccggcaaac caaccggata tggcaaatac ggcggacgga atgcaggggg acgatccgtc
     8941 ggcaggcggg gaaaatgccg gcaatacggc tgcccaaggt gcaaatcaag ccggaaacaa
     9001 tcaagccgcc ggttcttcag attccacccc tgcgtcaaac cctgccacta cgaatagcgg
     9061 cggtgatttt ggaaggacga acgtggctaa tggcatcaaa cttgatgggc cgtcgcaaaa
     9121 tataaccttg acccattgta aagacaaagt atgcggtagc gatttcttag atgaagaagc
     9181 accgccaaaa tcagaatttg aatcgttaga tgattctggg cgaattaata aatataaaaa
     9241 agatggacag gataaattta ctaatttggt tgcgacagaa gttaaagcta atggaactaa
     9301 caaatatgtc atcatttata aagacaagtc cacttcatct gcgcgagtta ggcgttctgc
     9361 acggtcgagg aggtcgcttc ctgccgagat gccgctaatc cccgtcaatc aggcggatac
     9421 gctgattgtc gatggggaag cggtcagcct gacggggcat tccggcaata tcttcgcgcc
     9481 cgaagggaat taccggtatc tgacttacgg ggcggaaaaa ttgcccggag gatcgtatgc
     9541 cctccgtgtg caaggcgaac cggcaaaagg cgaaatgctc gcgggcacgg cagtgtacaa
     9601 cggcgaagtg ctgcattttc atacggaaaa cggccgtccg tacccgacta ggggcaggtt
     9661 tgccgcaaaa gtcgatttcg gcagcaaatc tgtggacggc attatcgaca gcggcgatga
     9721 tttgcatatg ggtacgcaaa aattcaaagc cgccatcgat ggaaacggct ttaaggggac
     9781 ttggacggaa aatggcggcg gggatgtttc cggaaagttt tacggcccgg ccggcgagga
     9841 agtggcggga aaatacagct atcgcccgac agatgcggaa aagggcggat tcggcgtgtt
     9901 tgccggcaaa aaagagcagg attgatgttg atgccgtctg aagcgggggg cgttcagacg
     9961 gcattgtgat gttgggtcgc gatccggttc aagtcctcat cagttgataa acccgtcgtc
    10021 tttcattttc ttttcggaaa aatactgacc gcataccagt aaggcggcga ggatgatgac
    10081 aaacagcccc gccatagtca gtgtgatttt gaagaacaat tccgcttcga gaaaagaaaa
    10141 ccagagttgc acgatcagca gcagcaccca gccggcaaac agtccgagac aggtataaac
    10201 gccgagctta aacacgatcc cgctccaaaa tgacgacgta ggcctcgcgt ttgaaaaaca
    10261 gcagcatacg gcgcaaatct ttttccatcg ttacaacccg ccagccttcg ggggtttatt
    10321 tattgaggaa ctcgctgaat ttgatggggt tgacctttgc cgcgccgaaa aacaggctgg
    10381 acaacaggct ttcctgataa atgatgactt tgtattcttt cataggattc cttttaacat
    10441 aagggatcat cttttcagac gaccccttaa gttgctaatg atgcaaaaca gcaaacctta
    10501 tttcttattg cccaactgcg gcaataccga gccttcgccc aatgccaaca aaccgctttg
    10561 cgagtaaata cccagtttgg cgcgggtgtc ggtaatgtcg aggttgcgca tcgtgagctg
    10621 tccgatgcgg tcgagcgggg taaacgccgc accttcgact ttttccatac tcaggcgttc
    10681 gggttggtag gtcaggttgg gcgattcggt gttcagaatc gagtagtcgt tgccgcgccg
    10741 cagttcgagg gtaacttcgc cggtaacggc tttggcgacc cagcgttggg cggtttcgcg
    10801 caacatcaag gcttggctgt cgaaccagcg gccttggtag agcaggcggc cgaggcgcag
    10861 gccgttgatg cggtattgtt cgatggtgtc ttcgttgtgg atgccggtga tcaaacgctc
    10921 gtaggcgatg tggaacaacg ccatacccgg ggcttcgtag atgccgcgcg atttggcttc
    10981 gatgatgcgg ttttcgattt ggtcgctcat acctaagccg tggcggccgc cgatgcggtt
    11041 ggcttcaagg aagagttcga cagggtcggc gtattctttg ccgttcagtg caaccggcac
    11101 gccttcttca aagcgcacgc tgacttcttc gggtttgatt tcgacgtttt cgtcccagaa
    11161 ggcaacgccc ataataggtt tgacgatttt gatgcccgag ttcaaaaatt ccaaatcttt
    11221 ggcttcgtgg gtcgcgccga gcatattgga atcggtggaa taggcttttt ccaccgacat
    11281 tttgtagttg aaaccgttgg caatcaaaaa ttcgctcatt tcatgacggc cgccgagttc
    11341 gtcgataaat tgctggtcga gccagggttt gtagattttc agcgcgggat tggtgagcaa
    11401 accgtagcgg tagaaacgct cgatgtcgtt gcctttgtag gtgctgccgt cgccccaaat
    11461 attcacatcg tcttctttca ttgcggaaac aagcatagtg ccggttacgg cgcggcccag
    11521 aggcgtggtg ttgaaatagg cgatgccgcc ggtggaaacg tggaacgcgc cgcattggat
    11581 ggcggcgatg ccttcgtgtg ccagctgtgc gcggcagtca atcaggcggg cgttttccgc
    11641 accgtattcc atcgcttttt tgggaatggc gttgtagtcg tcttcgtcgg gctgaccaag
    11701 gttggcggtg taggcgtaag gcagcgcgcc tttgagtttc atccacaaca gtgcggcaga
    11761 ggtatcaaga ccgccggaga aggcgatgcc gactttttga ccgacgggga gggattgcag
    11821 aatagtatgg ttttggctca taagggtatc cttcggactt ggaaaaagga gttcgtcgtg
    11881 tttccttttc gattgaaacc ccgccacttg gacatctgtc cttcggggcg gtagaatcag
    11941 actttatttg ggaggggcgt aatcccttcc aaatcaggac ggcacatagg gcggtgcttt
    12001 atgtgtcgtc ccgtgtgttg aaacattatc ttataaatat aacccattgt cagccgttaa
    12061 ggctgtctga aaagaagctt tgcccgtttc gctgaaaatc ttttcagacg gtctttacgc
    12121 catcctcaac ctgactccct cccctgcggg ggagggttgg ggagagggtg cagacagcag
    12181 cccttaagca aacgctgcct gaaaaacgca agctcgaccc tctccctaac cctctcccat
    12241 gggagaggga acaaatcaca gcaccatcga cattttcaga cggcatcggt aaagaaaccc
    12301 atctatgaac gcgttcaacc tcaccatcat cggcgacgaa atcttacacg gcagccgcca
    12361 agacaagcat ttcgcctttt tcaaatccct gctcgaatcc aaagggctga agctcaatca
    12421 ggtgcaatat ctgcccgacg aacccgattt gctggtcagg caactgcgcc gcagcttttc
    12481 agacggcata ccgacgtttg ttaccggcgg catcggcgcc acgcccgacg accacacgcg
    12541 ccaagcggcc gccgacgctt tggagttgcc cgtcgtccgc catcctgaag ccgccgggtt
    12601 tatcgaaggc ataacccgga aacgcggcga gccgcttgat tcgccggaac acgcccaacg
    12661 cctgaaaatg gcagattttc ccgaaggcgc ggaattggtg cccaatccgt ttaacaacat
    12721 cgccggattt tccatccgcg agcattattt cttccctggt tttcctgtga tggcgcaccc
    12781 gatggcggaa tgggtattgg aaacttatta tgccgaccgc ttcaatcaaa ccgaacgcgg
    12841 cagccgcagc gtgtatgtgt ttgagcagcc cgaatcgcgc attacgccgc ttatggagca
    12901 tatcgagcaa acttatcccg gcgtgcgttc gtacagcctg cccagcgttg gttggacgca
    12961 ttcagacggc acgcaggtca aaccgcacat tgagttcggc atcaaagccg aaggagaagc
    13021 cgtcaacctt ttggacgcgg cgtgggcgga agtgttgcac agcttggacg gattgggagc
    13081 agagctgaaa aatcgggtaa actgaaaacc gtttgcccaa atcggacaat atttaaggaa
    13141 aaccatgcaa taccgaatca gaagagaaaa cgaagcaccc gaagcaaaaa atgcggggga
    13201 aaccttatgg gagcgcgaca tcatgcgcga agtgctgttg tccgcctatc aggacaggcg
    13261 cagggagcgg atgtggaaaa acatctggcg cgccgtcagc accctgattc tggttgccct
    13321 gattgcaggc attttccgaa aagacgaagc cgcattgcag ttggcgggca acacgccgca
    13381 taccgcagtc gtcaatctgt acggcgaaat cggcaacggt gtagaagacc aggtcaaaaa
    13441 actcaaagac ggtatggagg ccgcctacaa aaacccgcag gcaaaagcca tcgtcatccg
    13501 cgccaacagc cccggcggtt cgcccgtcgt ctccaacacc gcttttgaag aaatacgccg
    13561 tctgaaggcg cagcaccccg gcattcccgt ttatctcgtg gcggaagata tgtgcgcgtc
    13621 cggctgctac tacatcgcgg cggcggcgga caaaatctat gccgacccgt ccagcatcgt
    13681 cggcagcatc ggcgttatcg gcagcagttt cgacgcgacc ggcctgatgg aaaaaatcgg
    13741 cgtgaaacgc agggttaaaa ttgcaggcag caacaaaggc atgggcgatc cgttttcgcc
    13801 cgaaacgccc gaacagtcaa aaatctggga agaaatgctg accggcatac acggtgagtt
    13861 catcaaagcc gtcaaaaccg gacgcggcgg ccgcctcaaa ttccgacaat atcccgacgt
    13921 gttcagcggt cgcgtgtaca caggcgcgga tgcgctgaaa gtcgggctgg tggacggact
    13981 cggcaatatt tacagcgtgg cgcgagacgt ggtcaaagcc cccgatgtgg tggattacac
    14041 tccgaaggac gatttcggca gaatcttggg cagacgcttc ggcgcagaac tcaaagcaag
    14101 cgtgcgcgaa gccctgcagg ccgtccggta gcgttgttta cagtatttct aagggaatac
    14161 caaaataaaa atatacacaa aaacactcac ctccccagta tgctgccact aagtcagccg
    14221 cgtcactatt taccgtgcac ttaaagccgt aaaaggcagg ttgtcccggg aggttttgtt
    14281 catcctgata ccgatcggtt gttgttgctc aaaggacaga aggccgccga taaacgagat
    14341 tacctgtttg tcgctattga cgatttttat actctgccat tttgccggac aaaaccgcag
    14401 acagtgctgc caagtttctg accgaacatc tggctgaccc ctgcttgtac ctgattgagt
    14461 acgtttactc tgacaatgat aggtaatata aagagccgtc caacatgctt tcggtgcagc
    14521 ttgttataat aatgggattg gttggaggct tgcccgattt gcttgtccgc agaccaacgg
    14581 taaggcggag cgggttatcc gtaccttgat ggagatgtgg catgaggaac agtcgtttga
    14641 cagtccggaa catcggcaaa aggagttgtg ctgctttatt aatttagggg ctgccctaga
    14701 tcactaggga aattcaaatt aagttagaat tatccctatg agagaaagtc gtctaagacg
    14761 gtataaacaa aataaactca ttttgttatc gtggctatga tgtattggat gcgggcgaat
    14821 ttagccattt ccgtatcaat cacagcacac attttgccga acgacaaaac catattaatg
    14881 gaactgagaa cttttggaat caagcaaaac gtcatttacg caagtttaac ggcattccca
    14941 aagcgcattt tgagctgtat ttaaaggagt gcgaatggcg ttttaacaac agtgaaataa
    15001 aagttcttgt tccattttaa aacaattagt aaaatcgagt ttatcctagt tgtccaaggc
    15061 agcctcatct ctttatagat agctgcttac caagtatcct taattgctaa ttcaagccaa
    15121 tcttcaaaac tattaaaaaa ttcatggttt tctatattat ctaaattaat gacaataacc
    15181 ctagaatttc ctgttacatc attaccatca aaataagctt cccattctcc attactagcg
    15241 aatgggataa gatttaaatc aggcaggata gaatttctaa atatataagc tacttctgcc
    15301 ccttctgttc cataattctc aaactaccag tgaaaattat gtggctgaat ataaggtatt
    15361 aaagatgtat cgttagagat acgaacatac ttttcaggat aacaaaacct ttcaggaagc
    15421 atattactag gatataaatt aaattcattt tgaataatca tttcgttacc ttaggatcat
    15481 aaatactacc attaggaaac tgggtagacc aatttggtgg tgctagttca ttttcaattt
    15541 gttttttaat tctaaattga atagcttctc cccatgtttg cggcatctaa tgctgcttgc
    15601 tctgcatcaa tattactaaa aattggatta gtctcaaaaa tgaaaaatta ctgttttttt
    15661 attttttata gcatattcaa gtacatgaat aatatttttc actaagggct ctgtagaaaa
    15721 atctgaattt tgtaattttt cttttacagc caaaatactt tccttatcta ttagccaatc
    15781 cccctcactt ggtccaaata accaattatc ggtaatttca gaaacaagtc tccataatcc
    15841 acgattttga tattcaataa tttgactttc cgtaatttct acaactgttt cagattgaat
    15901 atttttataa gcccaatcaa tcatttcagt atattcacta attgaaacat tgtcttttaa
    15961 acagaaaatt aacaggttgt gttcattcat ttgtctaagt tcctgatatg agtttgttgt
    16021 aaataatctt ttgcttgctt accttgtaaa ttaaaggatt aaccgcacct tggatgatgc
    16081 cgcgaatatc atccatacgg ttaccccacc aaattttatt ggtgtatcgt taatcattta
    16141 tttcaaaaaa gttatttatt atcatattat cagggagcag gtcgttaaaa aaaaccccct
    16201 caaattccat taaattagta tttatccagc acataaacga accaaagcgt tctttccctg
    16261 agtgtaaata tttaaaatta aactcaaaat aagttttttt gcttagaaaa tttaatggtt
    16321 tttctatagt gaaaaaaaag cccttactcg ttttagttcg agatactatt ttaaataaat
    16381 ttttattttc aataaaataa gaatattcat ttactaagtt gttatgataa aaaagtaatt
    16441 ttattaaaat gttttcataa ttttctaggt tcatttctag ctcaatttaa aatgatttca
    16501 ttgttgccgt accaatgcga aatttaccat caacaaaatt tcccttcact gatacaggta
    16561 cgccatcaac ttgggtagtt ttattttcag gctgcctgaa agaaagatag gcaacttatt
    16621 actcatctta cgctagccct caataattga atacgttgca ctagaaccaa ttatttctat
    16681 gaatgtttgc tcaaacataa aaatatagta tttaaagaat tctctattca aaggaaaata
    16741 actttctttc aatatccatt ccgcataaca ctcatcaaga agttcaaaaa aagattgacc
    16801 accattttca tgaagcaaag ctctatctgc atcttccgaa attaaatcca taaaagtatc
    16861 tataaaatac gattcatcaa caaccctaaa atgaataaca tcttcaataa aaatttttat
    16921 ggttttatcc gtatttctat aaccaactgc tataacttct actttatcag agtttattgc
    16981 tgcattatca aaatagaaat agccagttgg gaaatctaag ctttttaatt tcataattta
    17041 tcgtgtattt ccatatctga tttttatttt tcggttatta ctaattgtta tttcaagtgt
    17101 tggttctcca ccactactat cattacgagc attaaccgca cgcctatcag gtaaattgcc
    17161 cgttatcgtt ccattaggac gtgtttgtac atttcgaaca ttcagactat taaaatcatt
    17221 tagtgcgtca ttgtaatccc ccactttatc atactgaaca gcgggacctt ttgttttact
    17281 cctttgagta gtattgctca aaatgtcttt gatttgtaaa tcactgcgca ccttaccggt
    17341 ttctatgcga gctgccacac tactcgggct ataggggtcg ctatcaatcg caggattacc
    17401 accgctggtt ttttgtccgc tagcctgttt cccaacctgc tgtactgtat cgccaatttc
    17461 atctaccgta cctaacgcct tagtctgcat atggcgaaca gcaggttttt tatagccggt
    17521 atttttaacc caatcccatt tagtcgggtt aagttctact tttttacctc tccaaaccgt
    17581 acctgcggcc tctgctacgg aaagggcagt ttgggctgtt gcagttatat tcggatgggc
    17641 atcagcccat tgtctggcag agttgatact gtcttttacc gcaaaagcac tgtcttgtaa
    17701 tgcggttgcg gccgcaagtt gtgcttcggg acttaaattt cctaaattat taataccttg
    17761 tagagtctgc tgcgcagaga tgtagcaact gtatttttac cccgtcgggt aaaaatacag
    17821 ttgctacggc tgctg
//
LOCUS       DAWFHY010000041        17623 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.041, whole genome shotgun sequence.
ACCESSION   DAWFHY010000041 DAWFHY010000000
VERSION     DAWFHY010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17623
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.041"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..564
                     /locus_tag="WGQ68_001201"
     CDS             <1..564
                     /locus_tag="WGQ68_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0714995.1"
                     /translation="VSVGYDFGGWRIAADYASYRKWNNNKYSVNTKEVSRNNSSGIWQ
                     ELKTENQENGTFHAASSLGLSAIYDFKLNDKFDKFKPYIGARVAYGHVKHQVHSVRKE
                     TTTTFSPPAQGATVPGKIVQGPTNKPAYHESNSISSLGLGVIAGVGFDITPKLTLDTG
                     YRYHNWGRLENTRFKTHEVSLGMRYHF"
     gene            complement(538..747)
                     /locus_tag="WGQ68_001202"
     CDS             complement(538..747)
                     /locus_tag="WGQ68_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714996.1"
                     /translation="MPSERLSDGIFPCVLNRVHETPDAFGGNVGCRAMFGGDQRTKYA
                     GKGLMPSEGSDGIGVGESEVVAHAQ"
     gene            complement(765..1322)
                     /locus_tag="WGQ68_001203"
     CDS             complement(765..1322)
                     /locus_tag="WGQ68_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0714997.1"
                     /translation="MDALKLLTNRRSSKKLKHPAPDAAELEQIFKAATQVPDHGNMRP
                     FRFTVIQGEVGLQRFRDVLKQTVAELNFGDDAMKKAEKVGNMAPMVIGVTFAPNRDVP
                     KPKPEWEQMLTAGCAAYALQLAATAQGFDNVWITGMWVNSPLLREAFGCADKDKIIGL
                     MMVGTPTEEVHKPKNTDLEAFVSHW"
     gene            complement(1398..3032)
                     /locus_tag="WGQ68_001204"
     CDS             complement(1398..3032)
                     /locus_tag="WGQ68_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipooligosaccharide phosphoethanolamine
                     transferase LptA"
                     /protein_id="HEZ0714998.1"
                     /translation="MIKPNLRPKLGSSALIAFLSLYSSLVLNYAFFAKVVELHPFNGT
                     GADIFLYTMPVVLFFLSNFVFHVIALPFVHKVLIPLILVISAAVSYQEIFFNIYFNKS
                     MLNNVLQTTAAESARLITPGYVLWIVCLGVLPALAYIAVKVKYRVWYKELLTRLVLAA
                     VSFLCALGIAMLQYQDYASFFRNNKSVTHLIVPSNFIGAGVSKYKDWKRSNIPYTQLD
                     MAVVQNRPAGSLRRFVVLVVGETTRAANWGLNGYSRQTTPLLAARGDEIVNFPQVRSC
                     GTSTAHSLPCMFSTFDRTDYDEIKAEHQDNLLDIVQRAGVEVTWLENDSGCKGVCGKV
                     PNTDVTSLNLPEYCRNGECLDNILLTKFDEVLNKNDKDAVLILHTIGSHGPTYYERYT
                     EAERKFTPTCDTNEINKCTRATLVNTYDNTVLYVDQFIDKVIRKLENRDDLESVVHYV
                     SDHGESLGENGMYLHAAPYAIAPSGQTHIPMVMWFSKAFRQHGGIDFQCLKQKAAENE
                     YSHDHYFSTVLGLMDISNSQTYRKEMDILAACRRPR"
     gene            complement(3106..3435)
                     /locus_tag="WGQ68_001205"
     CDS             complement(3106..3435)
                     /locus_tag="WGQ68_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0714999.1"
                     /translation="MFRCCLKRLQTASAVSAFSGGVLEVVRLCCRRLSVLFNPGVMLF
                     VPDCPVGGAVGFFQYEMLCPFFWQGRLQTGSNLAYDVFMSAGRLNGGRNPPHSRRFLA
                     LLCSVAL"
     gene            complement(3435..4541)
                     /gene="serC"
                     /locus_tag="WGQ68_001206"
     CDS             complement(3435..4541)
                     /gene="serC"
                     /locus_tag="WGQ68_001206"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEZ0715000.1"
                     /translation="MSLYPIYNFSAGPAVLPEAVLETARQEMLDYNGTGFPVMAMSHR
                     SEMFLSILHHAEQDLRQLLKVPDNYKILFLQGGATTQFNMAAMNLAHGFRTADAVVTG
                     NWSRIAYEQMSRLTDTEIRLAAHGGEQFDYLDLPPVETWDVAPDSAFVHFAVNETVNG
                     LQYREVPRLSEGMPPLVCDMSSEILSREFDVADYGLIYAGAQKNIGPAGVTVVIVRED
                     LLERCPNDIPDVFNYRSHLNRDGMYNTPSTYAIYMSGLVFRWLQAQGGVKKIEAVNRL
                     KAQTLYETIDGSGGFYINRIRPNARSKMNVVFKTASEDLDRRFVLEAELQGLCLLKGY
                     KSVGGMRASIYNAMPLEGVRALADFMRDFQRRYG"
     gene            4906..5337
                     /gene="rimP"
                     /locus_tag="WGQ68_001207"
     CDS             4906..5337
                     /gene="rimP"
                     /locus_tag="WGQ68_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003685973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEZ0715001.1"
                     /translation="MDIQTILEKTLPGLGYELVDFELTAQGTLRVFIDKESGITVEDC
                     ATVSNHLSRVFMVEDIDYKNLEISSPGLDRPLKKAADFVRFAGQNAKIKTRLPIDGQK
                     NFIGKIEGCENDTVTVSFDGKTVQIGLGNIDKARLRPEFKF"
     gene            5365..6867
                     /gene="nusA"
                     /locus_tag="WGQ68_001208"
     CDS             5365..6867
                     /gene="nusA"
                     /locus_tag="WGQ68_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEZ0715002.1"
                     /translation="MSREMLQLAEALASEKNVDAEVVFQALEFALSTAAKKKADREHM
                     DVRVQINRDTGEYQTFRRWLIVADEDYTYPDVEKTIEEIQEEIPGTAIQIGEYYEEQL
                     PNEGFGRQAAQTAKQIILQRIRDAEREQNLNEFLAVKEDIVSGTVKRVERHGIIVEVV
                     AGKLDALIPRDQMIPRENFRSGDRIRALFLRVEEIGNTGRKQVILSRTSGDFLVKLYA
                     NEVPEIADGMLEIRAVARDPGQRAKVAVKANDQRIDPQGTCIGVRGSRVNAVSNELSG
                     ERIDVVLWSPEPAQFVMSALSPAEVSRIVIDEDKHAVDVIVAEDQLALAIGRGGQNVR
                     LASDLTGWQLNIMTSAEADERNAAEDAAIRRLFMDHLNVDEETADVLVQEGFATLEEV
                     AYVPAAELLAIEGFDEEIVDMLRNRARDAILTMAIAAEEKLGEVSDDMRNLEGIDADM
                     LRSLAEAGITTRDDLAELAVDELIEITGVNEETAKAVILTAREHWFTEDK"
     gene            6879..9767
                     /gene="infB"
                     /locus_tag="WGQ68_001209"
     CDS             6879..9767
                     /gene="infB"
                     /locus_tag="WGQ68_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEZ0715003.1"
                     /translation="MSNTTVEQFAAELKRPVEDLLKQLKEAGVSKNSGSDSLTLDDKQ
                     LLNAYLTKKNGSNGGTISIRRTKTEVSTVDGVKVETRKRGRTVNIPSAEELAAQVKAA
                     QTQAAPVRPEQTAEDAAKARAEAAARAEARAKAEAEAAKLKAAKAGNKAKPAAQKPTE
                     AKAETAPVAAETKPAEESKAEKAQADKMPSKKPAEPKEKAAKPKHERNGKGKDAKKPA
                     KPAAPAVPQPVVSAEEQAQRDEEARRAAALRAHQEALLKEKQERQARREAMKQQAEQQ
                     AKAAQEAKTGRQRPAKPAEKPQAAAPALENKPVNPAKAKKENRRNRDDEGQGRNAKGK
                     GGKGGRDRNNARNGDDERVRGGKKGKKLKLEPNQHAFQAPTEPVVHEVLVPETITVAD
                     LAHKMAVKGVEVVKALMKMGMMVTINQSIDQDTALIVVEELGHIGKPAAADDPEAFLD
                     EGAEAVEAEALPRPPVVTVMGHVDHGKTSLLDYIRRAKVVQGEAGGITQHIGAYHVKT
                     PRGVITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTIEAIAHAKAAGVPI
                     VVAVNKIDKEAANPERIRQELTQHEVIPDDWGGTVQFIDVSAKKGTNIDALLEAVLLE
                     AEVLELAAPVDAPAKGIIVEARLDKGRGAVATLLVQSGTLKKGDMLLAGTAFGKIRAM
                     VDENGKSITEAGPSIPVEILGLSDVPNAGEDAMVLADEKKAREIALFRQGKYRDVRLA
                     KQQAAKLENMFNNMGETQAQSLSVIIKADVQGSYEALAGSLKKLSTDEVKVNVLHSGV
                     GGITESDVNLAIASGAFIIGFNVRADASSRKLAENENVEIRYYNIIYDAIDDVKAAMS
                     GMLSPEEKEQLTGTVEIRQVISVSKVGNIAGCMVTDGVVKRDSHIRLIRNNVVIHTGE
                     LSSLKRYKDDVKEVRMGFECGLMIKGFNEIMEGDQLECFDIVEVARTL"
     gene            complement(9978..11372)
                     /locus_tag="WGQ68_001210"
     CDS             complement(9978..11372)
                     /locus_tag="WGQ68_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase/DUF3482 domain-containing protein"
                     /protein_id="HEZ0715004.1"
                     /translation="MNKQPLSLAVVGHTNTGKTSLLRTLLRDSGFGEVKNAPSTTRHV
                     EEAAISDGADMLVFLYDTPGLEDAGGVLEWLETHTDTRSDGIERLQQFLGSHGAHHDF
                     NQEAKVLRQVLQSDMAMYVIDAREPVLDKYRDELTILSWCAKPVMPVFNFTGGQLPES
                     WTTMLARRNLHVFAGFDTVAFDFEGELRLWENLATMLPERSTLDRLTAMRRREWQRLD
                     GEARREIADFLLDAAAFRQEVDENEDTATVLQTMQAEIRQLERQMQQRLFALYRFYHS
                     EIDGGDWMPQAFRQDPFDSELLKQYGIRTGTGAATGALIGLGLDIATLGGSLGLGTAI
                     GGFLGGILPNTRTISDKLSGRQTLHTDPETLTLLAARALDLLHVLQTRGHAAQSDIEL
                     HSRKAPWDAARLPPELNKARSHWKWSSLNTHRPETSRAERAGYVEKLQIRLSGKYSEL
                     NLNQDKAASRRQYK"
     gene            complement(11446..12786)
                     /locus_tag="WGQ68_001211"
     CDS             complement(11446..12786)
                     /locus_tag="WGQ68_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2868 domain-containing protein"
                     /protein_id="HEZ0715005.1"
                     /translation="MLNPSRKLVELVRILEEGGFIFSGDPVQATEALRRVDGSTEEKI
                     IRRAEMIDRNRMLRDTLERVRAGSFWLWVAAATFAFFTGFSGTYLLMDNQGLNFFLVL
                     AGVLGMNTLMLAVWLAMLFLRVKVGRFFSSPATWFRGKDPVNQAVLRLYADEWRQPSV
                     RWKIGATSHSLWLCTLLGMLVSVLLLLLVRQYTFNWESTLLGDSSSVRLVEMLAWLPA
                     KLGFPVPDARAVIEGRLNGNIADARAWSGLLVGSIVCYGILPRLLAWVVCKILLKTSE
                     NGLDLEKPYYQAVIRRWQNKITDADTRRETVSAVSPKIVLNDAPKWAVMLETEWQDGE
                     WFEGRLAQEWLDKGVAANREQVAALETELKQKPAQLLIGVRAQTVPDRGVLRQIVRLS
                     EAAQGGAVVQLLAEQGLSDDLSEKLEHWRNALAECGAAWLEPDRAAQEGRLKDQ"
     gene            12929..13555
                     /locus_tag="WGQ68_001212"
     CDS             12929..13555
                     /locus_tag="WGQ68_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEZ0715006.1"
                     /translation="MYTGERFNTYSHLSGLILAAAGLVLMLLKTIGHGDGYRIFSVSV
                     YGISLLLLYLSSSLYHGIAAGKLKSILKKTDHCMIYVLIAGSYTPFALVSLRNGPGWT
                     VFSLSWLLAAAGIAQELTIGRKSEKRLLSIVIYVVMGWMVLAVMKSLTASLPSAGLAW
                     LAAGGMLYSVGIYWFVNDEKIRHGHGIWHLFVLGGSITQFVSVYGYVI"
     gene            complement(13619..15037)
                     /locus_tag="WGQ68_001213"
     CDS             complement(13619..15037)
                     /locus_tag="WGQ68_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0715007.1"
                     /translation="MENILSVLVGTVNRFLWDYLLIYALLGIGLFFTLYLGAPQITKL
                     GAGFKSVFGGLFAKGDKDDKSLSQFQALAVAISAQIGTGNVAGVATAITAGGPGAIFW
                     MWVSAVLGMSTIFAEALLAQKYRVVSHGKYIGGPAFYITHGLTPKIGRGAARFLSGFF
                     SIALIIALGFIGNATQANSIASAVTIAFDVPSLAVGIVLAVLAGMVVIGGVNRIANIA
                     QFVVPFMAVVYILCAVVILFEFSDHIVPMFNHIFTAAFNPEAVLGGAAGIGMREAIRF
                     GVARGLFSNEAGMGSTPHAHATADVKHPVQQGMAAFVGVFIDTILVCTATALIILLTD
                     ANLSGEQGAAVTQFAFNKAFPGFGSQLLAVCLTFFALTTIIGWYYFGESNIRFLFRGR
                     HLGIYRALVLLAIVLGTLGKVDLVWSLSDMFNGFMVIPNLIALFLLRKEIRAIYDDYL
                     AQKKAGRDLSYPYEFHEFHDKG"
     gene            15364..16092
                     /locus_tag="WGQ68_001214"
     CDS             15364..16092
                     /locus_tag="WGQ68_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004285004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEZ0715008.1"
                     /translation="MAGHSKWANIQHKKARQDAKRGKIFTRLIKEITVAARMGGGDPG
                     ANPRLRLALEKAAENNMPKDNVQRAIDKGTGNLEGVEYIELRYEGYGIGGAALMVDCL
                     TDNKTRTVADVRHAFTKNGGNLGTDGCVAFNFVHQGYLVFEPGVDEDALMEAALEAGA
                     EDVVTNDDGSIEVITAPNDWAGVKSALEAAGYKSVDGDVTMRAQNETELSGDDAVKMQ
                     KLIDALEDLDDVQDVYTSAVLNLD"
     gene            complement(16151..16639)
                     /locus_tag="WGQ68_001215"
     CDS             complement(16151..16639)
                     /locus_tag="WGQ68_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEZ0715009.1"
                     /translation="MTPLFRKAVWLLFAVSVCAFAGSLAAQYVLGMEPCVLCISQRLC
                     VLATALCTAIVLMCRPRRRAGGLFGTVFISIPAVTGISVAAYQLWLQSLPPGTAPSCG
                     APWTFRLKGWPLFDWFEPVVRGFGNCAEPDYLLGVALPVWSVAYFLAVVLTVWWAWAR
                     AK"
     gene            complement(17396..>17623)
                     /locus_tag="WGQ68_001216"
     CDS             complement(17396..>17623)
                     /locus_tag="WGQ68_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEZ0715010.1"
                     /translation="NIFEDFKKEVLKIPQIMECHLVSGEYDYLIKVRLPDMSAYRDML
                     GNILLQLPAASESRSYVVMEEVKENPVLDLD"
BASE COUNT         4653 a         4947 c         4433 g         3590 t
ORIGIN      
        1 gtgtcggtcg gctacgattt cggcggctgg aggatagcgg cagattatgc cagttacaga
       61 aaatggaaca acaataaata ttccgtcaac acaaaagagg tatcaagaaa caatagcagt
      121 ggcatctggc aagaactgaa gacggaaaat caggaaaacg gtacattcca cgccgcttct
      181 tctctcggct tatccgccat ttacgatttc aaactcaacg ataaattcga taaattcaaa
      241 ccctatatcg gtgcgcgcgt cgcctacgga cacgttaaac atcaggttca ttcggtgaga
      301 aaagaaacca cgactacttt cagtccacca gcgcaaggcg ctacagtgcc aggcaaaatc
      361 gtacaaggtc cgaccaacaa acctgcctat cacgaaagca acagtatcag cagcttaggt
      421 cttggtgtca tcgccggtgt cggtttcgac atcacaccca agctgacttt agacaccgga
      481 taccgttacc acaactgggg acgcttggaa aacacccgct tcaaaaccca cgaagtctca
      541 ttgggcatgc gctaccactt ctgattcccc gacaccgatg ccgtctgaac cttcagacgg
      601 catgagacct ttgcctgcgt acttggtacg ctggtcgcct ccgaacatgg cgcgacaccc
      661 gacatttccg ccgaacgcat cgggcgtttc atgaacccgg tttaaaacgc atggaaaaat
      721 gccgtctgaa agcctttcag acggcattgt gcttgagatt ccgtttacca atggctgaca
      781 aacgcttcca aatcggtatt cttgggctta tgcacttcct ctgtcggcgt gccgaccatc
      841 atcagcccga tgattttatc cttatccgca catccgaaag cctcacgcaa caaggggctg
      901 ttgacccaca tccccgttat ccagacattg tcgaaacctt gcgccgttgc cgccagttgc
      961 agcgcatacg ccgcacaacc cgccgtcagc atctgctccc attccggttt cggcttaggc
     1021 acatcgcggt tcggcgcaaa cgttaccccg atgaccatcg gcgccatatt gcccactttt
     1081 tccgcctttt tcatcgcatc gtcgccgaaa ttcaattcgg caaccgtttg cttcaacaca
     1141 tcgcgaaaac gttgcaatcc tacctcgcct tgaatcacgg taaaacggaa ggggcgcata
     1201 ttgccgtgat cgggaacttg ggttgccgcc ttaaatattt gttccaactc cgccgcatcg
     1261 ggggcggggt gcttcagctt tttggaagat cggcggttcg tcaataattt taaagcatcc
     1321 atatcgttat actccggtca tcggtcgggt gttcggacac atatgccgtc cgaaggcttc
     1381 agacggcata tccggcatca gcgcggacgg cggcaggctg ccaatatatc catttccttc
     1441 cgataggttt ggctgttgga aatgtccatc agccccaata ccgtgctgaa atagtggtcg
     1501 tgcgaatatt cgttttccgc cgctttttgt ttgaggcatt ggaaatctat gcccccgtgt
     1561 tggcggaagg ctttggaaaa ccacataacc atcgggatat gcgtctgccc ggaaggcgcg
     1621 atggcgtaag gcgcggcgtg caggtacatc ccgttttcgc ccaaactttc gccgtggtcg
     1681 gaaacataat gcaccacgct ttccaaatcg tcgcggtttt caagtttgcg gataaccttg
     1741 tcgataaact ggtccacata caaaaccgta ttgtcgtaag tgttgaccag cgtggcgcgg
     1801 gtgcatttgt tgatttcgtt ggtgtcgcag gtcggcgtga atttgcgttc ggcttcggta
     1861 tagcgttcgt aatacgtcgg cccgtggctg ccgatggtat gcaggattaa aaccgcgtct
     1921 ttatcgtttt tgttgaggac ttcgtcgaac ttagtcagca ggatattgtc gaggcactcg
     1981 ccgttgcggc agtattcggg caggttgagc gaggtaacgt cggtattcgg cactttgccg
     2041 cacacgccct tgcagccgga atcgttttcc aaccaagtaa cttccacgcc ggcgcgctgc
     2101 acgatgtcca gcaggttgtc ttggtgttcg gctttgattt cgtcataatc cgtgcggtcg
     2161 aaggttgaga acatacacgg cagggagtgc gcggtcgatg tgccgcagct tctgacctgc
     2221 gggaaattga caatttcatc gccgcgcgcg gcaagcagcg gcgtagtttg gcggctgtaa
     2281 ccgttcaaac cccagttggc ggcacgcgtg gtctcgccca cgaccagcac cacgaaacgg
     2341 cgcaggctgc cggccgggcg gttttgcacg accgccatat ccaattgcgt ataaggaata
     2401 ttggaacgct tccaatcttt gtatttcgac acgcccgcgc cgatgaaatt agacggcaca
     2461 atcagatggg ttactgattt attgttgcgg aaaaacgagg cgtaatcctg atattgcaac
     2521 attgcgatgc ccaacgcgca caaaaaggaa acggcggcaa gcacaaggcg cgtcaaaagc
     2581 tccttatacc aaacgcggta tttaaccttg acggcgatat acgccagcgc gggcaatacg
     2641 cccaaacata caatccacag cacatagccc ggcgtaatca ggcgcgcgct ttcggcagcc
     2701 gtagtttgca agacattatt caacatcgac ttgttgaaat agatattgaa aaatatttct
     2761 tggtaagaca ccgccgcact gataaccaat atcaacggaa tcaatacctt atgcacgaaa
     2821 ggcagggcaa tgacgtgaaa aacgaaatta cttaaaaaaa acagcaccac cggcatcgta
     2881 tagaggaaga tatccgcccc ggtgccgtta aaaggatgaa gctcgacaac tttggcaaaa
     2941 aaggcgtaat tcaataccag cgaggaatac agggaaagga aggcaatcag cgcggaagag
     3001 ccgagcttcg gcctcaggtt cggttttatc atttggaatg tgtcggataa gggttggaaa
     3061 aggcatccgg catttggaat ccggattatt gaaaaagatt cttaattata aggcaacgga
     3121 gcaaagcagg gcaagaaaac ggcggctgtg cggggggttc cgcccgccat tcaaacgtcc
     3181 ggcagacata aaaacatcgt aagcaagatt cgaaccggtc tgcaaccgcc cctgccagaa
     3241 aaacgggcaa agcatttcat attggaaaaa cccgaccgcg ccgccgacgg gacagtccgg
     3301 cacaaacagc atcacgcccg gattgaaaag gacggatagc cgccggcagc acaatcttac
     3361 cacctccaaa acgccgccgg agaacgccga aacagccgat gccgtctgaa gtcgcttcag
     3421 acaacatcgg aacatcaacc gtaacgccgt tggaaatcgc gcataaaatc tgccaaagcc
     3481 cgcacgcctt caagcggcat cgcattataa atgctggcac gcatcccacc gacagactta
     3541 tagcccttca acaggcacaa gccctgcaat tcggcttcca acacaaagcg gcggtccaag
     3601 tcttcgcttg cggttttgaa gaccacgttc attttggagc gcgcattcgg acggatacgg
     3661 ttgatataga aaccgccgct gccatctatc gtctcataca aggtttgcgc cttcagccga
     3721 ttgaccgctt caattttttt cacaccgccc tgcgcctgca gccagcggaa caccagcccc
     3781 gacatataaa tcgcgtaagt tgacggcgtg ttgtacatac cgtcgcggtt caaatgcgaa
     3841 cggtagttga acacatcggg aatatcgttc ggacaacgct cgagcaaatc ctcacgcaca
     3901 atcaccaccg taactcctgc cggcccgatg tttttctgtg cgcctgcgta aatcagtccg
     3961 tagtcggcaa catcaaactc gcgcgacaaa atctcgctgg acatatcgca caccagcggc
     4021 ggcatgcctt ctgaaaggcg cggcacttca cggtattgca gcccgttgac cgtttcattg
     4081 acggcaaaat ggacaaacgc cgaatcgggt gcaacatccc acgtttccac aggcggcagg
     4141 tcgagatagt cgaactgctc gccgccatgc gccgccaaac ggatttccgt atcggtcaaa
     4201 cggctcatct gttcataagc gatacggctc cagttgcccg ttaccaccgc gtcggcagtg
     4261 cggaaaccgt gtgccagatt catggctgcc atattaaatt gggttgttgc tccgccctgc
     4321 agaaacaata tcttatagtt gtcaggcact ttcaaaagct gcctcaaatc ctgttccgca
     4381 tgatgcagga tgctcaaaaa catttccgaa cggtggctca tcgccatcac gggaaaaccc
     4441 gtaccgttgt agtccaacat ttcctgccgc gccgtttcca acacggcttc gggcaatacg
     4501 gcagggccgg cggaaaaatt gtaaatcgga taaagagaca tgatgcagcc ttgattctga
     4561 acaataaccc gcctgatttt aggcttgcag caggctttgt gcaaggatgg gaaatacctg
     4621 tcctccgccc gattccatgc cgcccgaaca cggaaaacaa catcaatata ttgatttaca
     4681 aacataaaaa tcatgctagc gacaaataga tacatttgtt ttgtcaacaa tattcacgat
     4741 ttcccattac aaacctccct tacacccgct tttttccgtc ccaaaaacac aaaataaatc
     4801 aacactttca tttctccgca aaagcggtta taattacgcc gatttttcaa ctttgacgaa
     4861 aaatggccac tgggccattt ttttattatg tacataggga gcagcatgga tattcaaacc
     4921 atcctcgaaa aaaccctgcc cggcctgggc tacgaactgg tcgatttcga actgaccgcg
     4981 caaggaacat tgcgcgtgtt catcgacaaa gaaagcggca ttaccgtcga agactgcgca
     5041 accgtcagca accacttgag ccgcgtcttc atggttgaag acatcgacta caaaaacctg
     5101 gaaatttcca gccccggact cgaccgcccc ttgaaaaaag ccgccgactt cgtgcgcttt
     5161 gccggtcaga atgccaaaat caaaacccgc ctgccgatag acggtcagaa aaactttatc
     5221 ggtaaaatcg aaggctgcga aaacgatacc gttaccgtat ccttcgacgg caaaaccgta
     5281 caaatcggat tgggcaacat cgacaaagcc cgtctgcgcc ccgaattcaa attctaaaac
     5341 acaacaatat tggagatgtt caaaatgagt cgtgaaatgt tacagctggc agaagcactg
     5401 gcaagcgaaa aaaacgttga tgcggaagtc gtcttccaag cactggaatt cgccctgtct
     5461 accgccgcca agaaaaaggc agaccgcgaa cacatggacg tgcgcgtcca aatcaaccgc
     5521 gacaccggcg aataccaaac cttccgccgc tggctgattg tcgccgatga agactatacc
     5581 tatcccgatg tcgaaaaaac catcgaggaa atccaagagg aaattcccgg caccgccatc
     5641 caaatcggcg aatactacga agagcagctg cccaacgaag gcttcggccg ccaagccgcg
     5701 caaaccgcca aacaaatcat cctgcaacgc atccgcgatg ccgagcgcga gcagaatctg
     5761 aacgagtttc tcgccgtcaa agaagacatc gtgtccggca cggtcaaacg tgtcgaacgc
     5821 cacggcatca tcgtcgaagt cgttgccggc aaactggacg cgctgattcc gcgcgaccaa
     5881 atgattccgc gcgaaaactt ccgcagcggc gaccgcatcc gcgccctctt cctgcgcgtc
     5941 gaagaaatcg gcaacaccgg ccgcaaacaa gtcatcctga gccgtacttc cggcgatttc
     6001 ctcgtcaaac tgtacgccaa tgaagtacct gaaattgcag acggcatgct tgaaatccgc
     6061 gctgtcgccc gcgacccggg ccagcgtgcc aaagtcgccg tcaaagccaa tgaccagcgc
     6121 atcgatccgc aaggcacctg tatcggcgtt cgcggttcgc gtgtcaatgc cgtcagcaac
     6181 gaattgtccg gcgagcgcat cgacgttgtc ctttggtcgc ccgagcctgc ccaattcgtc
     6241 atgagcgcgc tctcacccgc cgaagtcagc cgcatcgtca tcgacgaaga caaacacgcc
     6301 gtcgatgtca tcgttgccga agaccagctc gcgctcgcca tcggacgcgg cggtcaaaac
     6361 gtgcgccttg cttccgacct gaccggctgg cagctcaaca tcatgacttc cgccgaggca
     6421 gacgaacgca atgcggcaga agatgccgcc atccgccgcc tgtttatgga tcacttgaac
     6481 gtggacgaag aaaccgccga cgtactggtt caggaaggtt ttgcaacctt ggaagaagtc
     6541 gcctatgttc ctgccgccga actgcttgcc attgaaggat ttgacgaaga aatcgtcgat
     6601 atgctccgca accgcgcccg cgatgccatc ctgaccatgg cgattgccgc cgaagaaaaa
     6661 ctgggcgaag tgtccgacga tatgcgcaac ctcgaaggca tagatgccga tatgctccgc
     6721 agccttgccg aagcaggcat taccacccgc gacgacttgg cagagcttgc tgtggacgaa
     6781 ctgattgaaa tcaccggtgt aaacgaagaa accgcaaaag ccgtcatcct gaccgctcgc
     6841 gaacactggt ttaccgaaga caaataaagg gggtacagat gagtaacaca accgtagaac
     6901 aatttgccgc cgagctgaaa cgccccgtcg aagacctgtt gaaacagttg aaagaagccg
     6961 gcgtcagcaa aaacagcggc agcgattccc tgacgctgga cgacaaacag cttctgaacg
     7021 cctacctgac caagaaaaac ggcagcaacg gcggcaccat cagcatccgc cgcaccaaaa
     7081 ccgaagtcag caccgttgac ggcgtaaaag tcgaaacacg caaacgcgga cgcactgtca
     7141 acattccttc tgccgaagaa ttggcagcac aggtaaaagc cgcccaaacc caagccgcac
     7201 ctgtccggcc ggagcagacg gcagaagacg cggcaaaagc ccgagccgaa gctgccgcac
     7261 gtgcagaagc ccgtgccaag gcagaagcgg aagcggcaaa actgaaagcg gcaaaagcag
     7321 gcaacaaagc caaacctgcc gcgcagaaac ccaccgaagc aaaagccgaa accgcacccg
     7381 ttgcggcgga aaccaaaccc gccgaagaaa gcaaagcgga aaaagcccaa gccgacaaaa
     7441 tgccgtctaa aaaacccgcc gagcccaaag aaaaagccgc caagccgaaa cacgagcgaa
     7501 acggcaaagg caaagatgcc aaaaaaccgg cgaaacctgc cgcacctgcc gtgccgcaac
     7561 ccgtggtcag cgcggaagaa caggcgcaac gcgatgaaga agcacgccgt gccgccgcac
     7621 ttcgcgccca ccaggaagcc ctgttgaaag agaaacagga acgccaggca cgccgcgaag
     7681 ccatgaaaca acaggcagaa caacaggcaa aagccgcaca ggaagccaaa accggcagac
     7741 agcgtcccgc caaacctgcc gagaaaccgc aggcagccgc gccggccctc gaaaacaagc
     7801 ctgtcaatcc ggcaaaagcg aaaaaagaaa accgccgcaa ccgcgatgac gaaggtcaag
     7861 gccgcaacgc caaaggcaaa ggcggaaaag gcgggcgcga ccgcaacaat gcacgcaatg
     7921 gcgacgacga gcgcgtacgc ggcggtaaaa aaggcaaaaa actcaaactc gagccgaacc
     7981 aacacgcctt ccaagcaccg accgaacctg tcgtacacga agttttggtg cctgaaacca
     8041 ttaccgttgc cgacttggca cacaaaatgg cagtcaaagg cgtggaagtg gtcaaagccc
     8101 tgatgaagat gggcatgatg gttaccatca accaatccat cgaccaagac accgccctga
     8161 ttgtcgtcga agaactcgga cacatcggca aacccgccgc cgccgacgat cccgaagcct
     8221 tcttggacga gggcgcggaa gcggtggaag ccgaagcatt gccgcgtccg ccggtggtta
     8281 ccgtgatggg gcacgtcgac cacggtaaaa cctcgctgct ggactacatc cgccgcgcca
     8341 aagtggtgca gggcgaagca ggcggcatta cgcagcacat cggcgcgtac cacgtcaaaa
     8401 cgccgcgcgg cgtgattacc ttcttggata ccccgggcca cgaagcgttt accgccatgc
     8461 gcgcacgcgg cgcgaaagcg accgacatcg tgattctcgt ggttgccgca gacgacggcg
     8521 tgatgccgca aaccatcgaa gcgattgccc acgccaaagc ggcaggcgta ccgattgtgg
     8581 ttgccgtcaa taaaatcgac aaagaagccg ccaaccccga acgcatccgt caggaactga
     8641 cccaacacga agtcatcccc gacgattggg gcggcacggt tcaattcatc gatgtttccg
     8701 ccaaaaaagg gacaaacatc gacgcgctgc tcgaagccgt cttgctcgaa gccgaagttt
     8761 tagaactggc cgccccggtc gatgcgcccg ccaaaggcat catcgtcgag gctcgtttgg
     8821 acaaagggcg cggcgcggtt gccacattgc tggttcaaag cggtacgctg aaaaaaggcg
     8881 acatgctgct ggccggtacg gcattcggca aaatccgcgc gatggtcgat gaaaacggca
     8941 aatccattac cgaagccggc ccgtccatcc cggtcgaaat cctcggcttg tccgacgtac
     9001 ccaatgcggg cgaagacgcg atggtattgg cggacgagaa aaaagcgcgc gaaatcgccc
     9061 tcttccgcca aggcaaatac cgcgacgtgc gccttgccaa acagcaggcg gcgaagctgg
     9121 aaaatatgtt caacaatatg ggcgaaaccc aggcccaatc tttgtcggtc atcatcaagg
     9181 cagacgttca gggctcttac gaggctttgg cgggcagcct gaaaaaactg tccaccgacg
     9241 aggtgaaagt gaacgtgttg cacagcggcg tgggcggcat taccgaatcg gatgtcaacc
     9301 ttgccatcgc ttcgggcgcg ttcattatcg gctttaacgt gcgtgcagat gcctcttcgc
     9361 gcaaacttgc cgaaaatgaa aacgtggaaa tccgctacta caacatcatc tacgatgcca
     9421 tcgacgacgt gaaagcggcg atgagcggta tgctttctcc cgaagagaaa gaacagctta
     9481 ccggcacggt cgaaatccgt caggtcatca gcgtttccaa agtcggcaac attgcaggct
     9541 gtatggttac cgacggcgtg gtcaaacgcg attcccatat ccgcctcatc cgcaacaacg
     9601 tggtcatcca taccggcgag ctgtcgtctt tgaaacgcta caaagacgat gttaaagaag
     9661 tccgtatggg cttcgagtgc ggcctgatga ttaaaggctt caatgaaatt atggaaggcg
     9721 accaactgga atgcttcgac atcgtcgaag ttgcacgcac cctgtgattt tgatgcggaa
     9781 aaatgccgtc tgaagcgttc agacggcata cgaaacgggt tctgtatcat acagaacccg
     9841 ttttttgtcg caaatcggct tcagacagcc ctcttgcctt atcccgattt gaatctgact
     9901 tgccatacaa acaggcttca gacggcatta tagtgaatta aatttaaacc ggtacagcgt
     9961 tggctcgcct tgccgtacta tttgtactgt ctgcggctcg ccgccttgtc ctgatttaaa
    10021 tttaattcac tatattttcc cgacaggcgg atttgaagtt tctccacata tccggcgcgt
    10081 tcggcgcggc tggtttcggg gcggtgcgta ttgagcgacg accatttcca atgactgcgg
    10141 gctttgttga gttcgggcgg gagtctggcg gcgtcccacg gggctttgcg gctgtgcagc
    10201 tcgatatccg actgtgccgc gtgtccgcgc gtttgcagga cgtggagcaa atcgagggcg
    10261 cgggcggcga gcagggtcag ggtttcaggg tcggtgtgca aggtttggcg gccggagagt
    10321 ttgtcggaaa tggtgcgggt attgggcagg atgccgccca aaaagccgcc gattgccgta
    10381 cccaatccga gcgagccgcc gagtgtggcg atgtccagcc ccaagccgat gagcgcgccg
    10441 gttgccgcgc ccgtgccggt gcggatgccg tattgtttga gcaattcgct gtcgaacggg
    10501 tcttggcgga aggcttgcgg catccagtcg ccgccgtcga tttcgctgtg gtagaaacgg
    10561 tagagggcaa acagccgctg ctgcatctgc cgttcgagtt ggcgtatttc cgcctgcatg
    10621 gtttgcagca cggtggcggt atcctcgttt tcgtccactt cctgcctgaa ggcggcggca
    10681 tcgagcagga aatcggcgat ttcgcggcgc gcttcgccgt ccagccgctg ccattcgcgc
    10741 cggcgcatgg ctgtcaggcg gtcaagtgtg ctgcgttcgg gcaacatggt ggcgaggttt
    10801 tcccacaggc gtagttcgcc ttcaaaatca aaggcgacgg tgtcgaaccc tgcgaaaacg
    10861 tgcaggtttc tcctcgccag catggttgtc cacgattcgg gaagctgtcc gccggtaaag
    10921 ttgaacacgg gcataaccgg tttggcacac catgaaagga tggtcaattc atcccgatat
    10981 ttgtcgagga cgggttcgcg cgcgtcgatg acgtacattg ccatatcgct ttgcaagact
    11041 tgccgtaaga ctttggcttc ctgattgaaa tcatggtgcg caccgtggct gccgagaaac
    11101 tgttgcagcc gctcgatgcc gtctgaacgc gtatccgtat gggtttccag ccattccaaa
    11161 acaccgcccg cgtcttcgag tccgggcgtg tcatacagga aaaccagcat gtccgcgccg
    11221 tcgctgatgg cggcttcttc gacatggcgc gtagtggacg gggcattttt gacttcgccg
    11281 aagccgctgt cgcgcaatag ggtgcgcagg agcgaggttt tgccggtatt ggtatgcccg
    11341 acgacggcga gggaaagggg ttgtttgttc atgatgtttt tgaagaatgg attttcagac
    11401 ggtctttttt cagaatggcg gcttaacaga acatttcaag tgagtttatt ggtctttcaa
    11461 acgcccttcc tgcgccgccc tgtcaggctc aagccacgcc gcgccgcatt cggccagcgc
    11521 gttacgccaa tgttccagct tttccgaaag gtcgtctgaa agcccctgtt ccgccaaaag
    11581 ctgcaccacc gcgccgccct gcgccgcttc cgaaagtcgg acgatctgcc gcaacacgcc
    11641 gcggtcgggc acagtttggg cgcgcacgcc gataagcagt tgcgccggtt tctgcttcag
    11701 ctctgtctcc agcgcggcaa cctgttcccg attggcggca acgcccttat ccagccattc
    11761 ctgcgccagc ctgccctcga accattcgcc gtcctgccat tcggtctcca gcatgaccgc
    11821 ccatttcggc gcatcgttca agacgatttt cggcgaaacg gcggacacgg tttcccgacg
    11881 cgtatccgca tcggtgattt tgttctgcca gcggcggatg accgcctgat aatagggctt
    11941 ttccaaatcc aagccgtttt cgcttgtttt caaaaggatt ttacacacta cccaagccaa
    12001 gaggcgcggc aggatgccgt agcagacgat actgccgacc agcagccccg accaagcccg
    12061 cgcatcggcg atgttgccgt tcagacggcc ttcgatgacc gcccgcgcat caggcacggg
    12121 aaaacccagt ttcgcaggca gccatgccaa catttccacc agccgtaccg aagacgaatc
    12181 gcccaacagc gtgctttccc agttgaacgt atattgccgc accaaaagca gcaacaatac
    12241 cgacaccagc attccgagca gcgtgcagag ccacaggctg tgcgacgttg cgcctatttt
    12301 ccaacgtacc gaaggttgcc gccactcgtc cgcatacagc cgcaacaccg cctgatttac
    12361 agggtctttg ccccgaaacc acgtcgccgg actgctgaaa aaacgcccca ctttcacgcg
    12421 caggaacaac attgccaacc atactgccag catcagcgta ttcatgccca acacgcccgc
    12481 caaaaccaaa aagaaattca gaccctgatt gtccattaga agataagtac ctgaaaaacc
    12541 ggtaaaaaac gcaaacgtcg ccgccgccac ccacaaccag aacgaccccg cacgcacacg
    12601 ttccaacgtg tcccgcagca tacggttcct gtcgatcatc tccgcccgac ggatgatttt
    12661 ttcctccgta ctgccgtcca cgcggcgcaa agcctccgtc gcctgcacgg gatcgccgct
    12721 gaaaataaag ccgccttctt ccaaaatacg gaccagctca accagttttc gggatggatt
    12781 caacataaaa tgccgtctga aaataaaaaa cagattttaa cacacgaatt ttcaagaata
    12841 ttcacagtgt aggcaaagag taaatcccac acagaggcaa aagtatcggc gtaaactgac
    12901 tgcctctact ttcccgaaag attgtgcgat gtatacaggc gaacgcttca atacttacag
    12961 ccatttgagc ggtttgattc tggcggcggc aggtttggtg ctgatgctgc tgaaaaccat
    13021 aggacacggg gacggctacc gtatcttcag cgtatcggtt tacggcatca gccttcttct
    13081 gctctatttg agttcctcgc tgtaccacgg aattgcagcc ggaaaactga aaagcatttt
    13141 gaaaaaaacc gaccactgca tgatttatgt cctgattgcc ggaagctaca caccgtttgc
    13201 actggtttct ttgagaaacg ggccgggctg gacggtattt tcactgtcct ggctgctggc
    13261 ggctgcagga atcgcacaag aactcaccat cggacggaaa agcgaaaaac gtctgctgtc
    13321 tattgtgatt tatgtcgtca tgggttggat ggtcttggcg gtaatgaaat ccctgacagc
    13381 ctcactcccg tcggcaggac tggcttggct ggcggcaggc ggtatgctgt acagcgtcgg
    13441 catttactgg tttgtaaacg atgaaaaaat ccgacacggg cacggaatct ggcatctgtt
    13501 cgtattgggc ggcagcatca cccaatttgt cagtgtgtac ggttatgtaa tctgaatgcc
    13561 gtctgaaaaa caaaacctcc cgatcctgaa gattgggagg ttttctgttt gccggacatc
    13621 agcccttgtc gtggaactcg tggaattcat acggataaga caaatcccga cccgcttttt
    13681 tctgtgccaa ataatcatca taaatggcac ggatttcctt acgcaacaaa aacagggcta
    13741 tcaagttcgg gataaccata aagccgttga acatatcgga caggctccaa accaaatcga
    13801 ctttgccgag cgtacctaaa acgatggcaa gcagaaccaa tgcgcgatag atgcccaagt
    13861 gtcttcccct gaaaagaaaa cggatgttgg actcaccgaa ataataccag ccgataatgg
    13921 tcgtcaaggc gaagaaggtc agacacacgg caagcaattg cgaaccgaag cccggaaatg
    13981 ccttgttaaa ggcaaattga gtaaccgccg cgccctgttc gcccgaaagg ttggcatcgg
    14041 tcagcaggat aatcagcgca gttgccgtac ataccaaaat cgtatcgata aacacaccga
    14101 caaatgccgc cataccttgc tgcacaggat gcttcacatc cgcagtcgcg tgggcgtgcg
    14161 gagtcgaacc catccccgcc tcattggaaa acagaccgcg cgccacgccg aaacgtatcg
    14221 cttcgcgcat accgataccc gccgcaccgc ccaaaacggc ttcgggattg aaggcggcgg
    14281 taaagatgtg gttgaacatc ggcacaatat ggtcggaaaa ttcaaacagg ataacgacgg
    14341 cgcacaaaat ataaacaacc gccataaacg gcacgacaaa ttgggcaata ttggcaatac
    14401 ggttcacgcc gccaatcaca accatgcccg caaggacggc aagcacaata ccgactgcca
    14461 aagaaggcac atcaaatgca atggtaacgg cagaagcaat ggagtttgcc tgtgtcgcat
    14521 taccgataaa gcccaatgcg ataatcaacg caatggaaaa gaaaccggac aaaaaacgcg
    14581 ccgctcccct gccgattttc ggagtcagac cgtgggtgat gtagaacgcc ggcccgccga
    14641 tgtatttgcc gtggctgacg acgcggtatt tctgcgccag cagtgcctcc gcaaaaatcg
    14701 tggacatccc caaaacggca gaaacccaca tccaaaaaat cgcgcccggc ccgcctgcgg
    14761 tgatggcggt cgccacgccg gcgacgttgc ccgtaccgat ttgcgcggat atggcaaccg
    14821 ccaacgcctg aaactgcgat aaagacttgt cgtctttatc gcctttggca aacaagccgc
    14881 cgaatacgga tttgaatccc gcgcccagct tggtaatctg cggcgcacca agatacagcg
    14941 taaaaaacag gccgataccc aaaagcgcgt aaatcagcag gtagtcccaa aggaaccgat
    15001 tgactgtacc caccagaaca gacaatatat tttccataaa ataaacctta tcttacaatt
    15061 aaaatgactg ccttccaaaa gacattccaa taaggaaaca cggcgagcag accgtatttg
    15121 ccgcaacaga tgccttaaat tgtcaacaat cggggagaag ctgcgccatc ataccgtaaa
    15181 atatcgttaa ttaaaacatt tctttatttt taagcggaaa gcggaggaaa tcgctttcag
    15241 acagcataga caacggcacg gcataaaaca ggatattttg ggtacttgca acttatgtta
    15301 aaatgccgac cgtaaaaaat ctgacaaaaa cagattaatt atttgaaata agaaaggaaa
    15361 tttatggcag gccatagcaa gtgggcgaat atccagcata aaaaagcccg tcaggatgcc
    15421 aaacgcggca aaatcttcac ccgtttaatc aaagaaatca ccgttgcggc gcgtatgggc
    15481 ggcggcgacc ccggtgccaa cccgcgcctg cgcctggctt tggaaaaagc agccgaaaac
    15541 aatatgccca aagacaatgt gcaacgcgcc atcgataaag gtacgggcaa cttggaaggc
    15601 gtggaataca tcgagttgcg ctacgaaggc tacggcatcg gcggcgcggc tttgatggtg
    15661 gactgcctga ccgacaacaa aacccgcacc gttgcggacg tacgccacgc gtttaccaaa
    15721 aacggcggca acttgggtac cgacggctgc gtggcgttca acttcgtgca tcagggctat
    15781 ttggtattcg aacccggcgt tgacgaagac gcgctgatgg aagcggcttt ggaagccggt
    15841 gcggaagacg tggttaccaa cgacgacggt tccatcgaag tcattaccgc gccaaacgat
    15901 tgggcgggcg taaaatccgc tttggaggcg gcaggttaca aatccgttga cggcgacgtt
    15961 acgatgcgcg cccaaaacga aaccgaactc tccggcgacg atgccgtcaa aatgcaaaaa
    16021 ctgattgacg cgctggaaga tttggacgac gtgcaagacg tttacacttc cgccgtattg
    16081 aatttggact gatacgcagc acagcagaca tacaataaaa tgccgtctga acctttcaga
    16141 cggcattgat ttatttagcc cttgcccacg cccaccaaac cgtcaggaca accgccagaa
    16201 aatacgccac actccaaaca ggcaaagcaa cccccagcag ataatccggt tcggcacaat
    16261 ttccgaaccc gcgcacgaca ggctcgaacc aatcaaacaa aggccagccc ttcaagcgga
    16321 acgtccacgg cgcaccgcac gaaggagccg tacccggcgg cagcgactgc aaccacaact
    16381 gatatgccgc aacagaaata cccgtaacgg ccggaatgct gataaagaca gtaccgaaca
    16441 aaccgcccgc ccttcttctt ggtctgcaca tcaggacaat tgccgtacac aatgcggttg
    16501 ccaaaacgca taaccgctga ctgatacaca aaacgcaagg ctccataccc aaaacatact
    16561 gtgccgccaa agaaccggca aatgcacaga ccgaaacggc aaacagcagc caaacggctt
    16621 ttctaaataa cggggtcatt ttctcaacac accaatcaaa ataccgatat gccgattttg
    16681 ctggatatat cccgagatgg caagggacga atcgacacca gacaggtgcg gacgatttcg
    16741 gttttcccga tatgcacaag acctaacaag ggacaaaacg gcgatttgcc cgcacaatgc
    16801 ccgcaaaaaa atacatctag aggatttgaa tttcggcaaa tttagcgaat caaaagttta
    16861 tttcaatgaa atcatatgat ttttttgaat aagcggattg atgggttttt gaaggaattt
    16921 gttactggat agccatcggg caagtttttt gcaaaatttg aatcggtcgg gtaaattttc
    16981 aaaaaataat tgacagcgga taagaaacgg cggataattc ccgccgtcga gttgcttgat
    17041 gcagcttgat ttttctcctc tatttctcct ttgtagactt ggcacacatt caactggatg
    17101 tgtgcatttt tttatctccg ctttttttga aatttaatta cttttattgt ttgaaattcc
    17161 attctttaag aaatttaaca agaaaaacgc ttgctttttg accggaaaag ctgcataatt
    17221 cattccgtta gctggttcga gtagtcagtt aatagtttct cctctatttc tcctttgtag
    17281 acttggcaca cattcaaccg gatgtgtgca tttttttatc tgaagcaaca agcctctgtg
    17341 cgtgatgttg ttatgtttca tttaggtgtc aaaccgcata gccagcctga aatattcaat
    17401 ccaaatccaa aaccggattt tctttgacct cctccatcac aacataactc ctactctccg
    17461 aagcggcagg aagttgtaac aaaatattgc cgagcatatc ccgataggca gacatatcgg
    17521 gcaaacgtac tttaatcaga tagtcgtatt cgcccgacac caagtggcat tccataattt
    17581 gcggaatttt cagcacttct tttttgaaat cttcgaaaat att
//
LOCUS       DAWFHY010000042        17425 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.042, whole genome shotgun sequence.
ACCESSION   DAWFHY010000042 DAWFHY010000000
VERSION     DAWFHY010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17425
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.042"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(405..1817)
                     /gene="trkA"
                     /locus_tag="WGQ68_001217"
     CDS             complement(405..1817)
                     /gene="trkA"
                     /locus_tag="WGQ68_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEZ0715011.1"
                     /translation="MKILILGNGQVGSTVAQNLAAIPNNDVTVIDIDEKALQETGSRL
                     DVQTVFGNGASPFTLERAGAEDADLLLALSRSDETNIVACKVAADLFNIPGRIARVRS
                     SEYLEYLSPKLENNENGSLSIFGITETISPEQLVTEQLAGLIDCPGALQVLRFADDRV
                     RMVIIQARRGGLLVGRSIADIAQDLPDGADCQICAVYRNNRLIVPAPQTVIIEGDEIL
                     FAAAAENIGAVIPELRPKETSTRRIMIAGGGNIGYRLAKQLEHAYNVKIIECRPRRAE
                     WIAENLDNTLVLQGSATDETLLDNEYIDEIDVFCALTNDDESNIMSALLAKNLGAKRV
                     IGIVNRSSYVDLLEGNKIDIVVSPHLITIGSILAHIRRGDIVAVHPIRRGTAEAIEVV
                     AHGDKKTSAIIGRRISGIKWPEGCHIAAVVRAGTGETIMGHHTETVIQDGDHIIFFVS
                     RRRILNELEKLIQVKMGFFG"
     gene            complement(1915..3438)
                     /locus_tag="WGQ68_001218"
     CDS             complement(1915..3438)
                     /locus_tag="WGQ68_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HEZ0715012.1"
                     /translation="MTVIKQEDFIQSICDAFQFISYYHPKDYIDALYKAWQKEENPAA
                     KDAMTQILVNSRMCAENNRPICQDTGIATVFLKVGMDVQWDADMSVEEMVNEGVRRAY
                     TWEGNTLRASVLADPAGKRQNTKDNTPAVIHMSIVPGGKVEVTCAAKGGGSENKSKLA
                     MLNPSDNIVDWVLKTIPTMGAGWCPPGILGIGIGGTPEKAVLMAKESLMSHIDIQELQ
                     EKAASGAELSTTEALRLELFEKVNALGIGAQGLGGLTTVLDVKILDYPTHAASKPIAM
                     IPNCAATRHVEFELDGSGPVELTPPRVEDWPDLTYSPDNGKRVDVDKLTKEEVASWKT
                     GDVLLLNGKILTGRDAAHKRLVDMLNKGEELPVDFTNRLIYYVGPVDPVGDEVVGPAG
                     PTTATRMDKFTRQMLEQTDLLGMIGKSERGAATCEAIADNKAVYLMAVGGAAYLVAKA
                     IKSSKVLAFPELGMEAIYEFEVKDMPVTVAVDSKGESIHATAPPQWQAKIGIIPVES"
     gene            complement(3745..4551)
                     /locus_tag="WGQ68_001219"
     CDS             complement(3745..4551)
                     /locus_tag="WGQ68_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HEZ0715013.1"
                     /translation="MNMKKWIAAALACSALALSACGGQGKDAAAPAANPDKVYRVASN
                     AEFAPFESLDSKGNVEGFDVDLMNAMAKAGNFKIEFKHQPWDSLFPALNNGDADVVMS
                     GVTITDDRKQSMDFSDPYFEITQVVLVPKGKKISSSDDLKNMNKVGVVTGYTGDFSVS
                     KLLGNDNPKIARFENVPLIIKELENGGLDSVVSDSAVIANYVKNNPTKGMDFVTLPDF
                     TTEHYGIAVRKGDEATVKMLNDALKKVRESGEYDKIYAKYFAKENGQAAK"
     gene            complement(4603..4854)
                     /locus_tag="WGQ68_001220"
     CDS             complement(4603..4854)
                     /locus_tag="WGQ68_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715014.1"
                     /translation="MGSIAQRLEYLEEANDVLRMQNHVLSTAFKALIRALPADTAEVA
                     VESIQLAFEDALAELSYEDSPHTDLFHDVTYAFFREKER"
     gene            4954..5715
                     /locus_tag="WGQ68_001221"
     CDS             4954..5715
                     /locus_tag="WGQ68_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715015.1"
                     /translation="MDANKEPNWEKRLENLRQTRRMKAVPTYAPLSRPSETGTDAPVP
                     KLDDESRARVLKAYLKKWQEEHNAAVADCGEEVETDPMIVLQENWLNAQASLQMSVSE
                     KHIESRRRILFDAKKDSAEFETGQIEGGENADAAESGTAADGVAENGEGGAEEALMPV
                     QINILNPKAVNRREVFCLSEQELTERLIKRLRPHLTDTVNGIIRTAVQKQMAVFTYQL
                     QQMLHEQAGAVVEDVLEHDVRKILNDIKYELKYKR"
     gene            6013..7182
                     /gene="metZ"
                     /locus_tag="WGQ68_001222"
     CDS             6013..7182
                     /gene="metZ"
                     /locus_tag="WGQ68_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HEZ0715016.1"
                     /translation="MSKKLHPQTLAIRGGKEQTEYREHNQALFLTSSFMWDNAQHAAD
                     LFSKKIKGFTYTRTANPTTAAFEKRIAALEGAERAVATSTGMSAIQAAFFTFLQAGDH
                     VISSRSLFGTTVGFINNIVTKFGIEVSHVSPTDINEWKAAVKANTKLLFLETPSNPLG
                     EVADLEALAELAHGIGALLVVDNSLLSPVGSQPLKHGADISVSSATKAIDGHGRVMGG
                     VLAGSEELMAQVAVYCNSCGLAMSPFNAWQLLSGVETLSLRMEKQFDNALKIAQWLQA
                     QPQVQAVYYTGLPDHPQAELIRKQQNGGGIVIGFEVADQAAAWKVVDGVELFSRTANL
                     GDVRSTITHPWTTTHGRMQPEEKLAANIRPGLVRLSVGLEYVGDLIDDLKQALAR"
     gene            7277..7483
                     /locus_tag="WGQ68_001223"
     CDS             7277..7483
                     /locus_tag="WGQ68_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2788 domain-containing protein"
                     /protein_id="HEZ0715017.1"
                     /translation="MDEAVFADWALKICLTGLIIFLGFIVWNLGKESKAGKFGIAILF
                     LVLGLGVFGFVFKELLIKFLVLPK"
     gene            complement(8480..9232)
                     /locus_tag="WGQ68_001224"
     CDS             complement(8480..9232)
                     /locus_tag="WGQ68_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEZ0715018.1"
                     /translation="MTDILIDNTATETVRTLIRAFPLVPVSQPPEQGSYLLAEHDTVS
                     LRLVGEKSSVIVDFASGAAQYRRTKGGGELIAKAVNHTAHPTVWDATAGLGRDSFVLA
                     SLGLAVTAFEQHPAVACLLSDGIRRALLNPETQDTAAHINLHFGNAAEQMPALVQTQG
                     KPDIVYLDPMYPERRKSAAVKKEMTYFHRLVGEAQDEAALLHTARQTAKKRVVVKRPR
                     LGEHLAGQAPAYQYTGKSTRFDVYLPYGTDKG"
     gene            complement(9336..9803)
                     /locus_tag="WGQ68_001225"
     CDS             complement(9336..9803)
                     /locus_tag="WGQ68_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEZ0715019.1"
                     /translation="MTELQHPVLVVDDETDILDLMEMTLMKMGLRVHTASGVAEAKNK
                     LDSQRYSLVLTDMRMPDSSGLEVVQHINSRLLDTPVAVITAFGNADQAQEALRCGAFD
                     PDTMQIQDYLDQIERDIIEQTLKQTEGNRTQAAKRLGISFRSMRYRMERLNIG"
     gene            complement(9855..11372)
                     /locus_tag="WGQ68_001226"
     CDS             complement(9855..11372)
                     /locus_tag="WGQ68_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEZ0715020.1"
                     /translation="MVISNTRELEKLKDRIPNLINIARIAIVFPLLIMHIFGLEVSGH
                     ENLHASWTAWAFYVWLAISCWLIFFSIIHPHWQWQSLKMPRFSAVADITMIGVLTYLF
                     GGIDSGFGILILPFVVCSCLLSYGRYPLLYASYAAILLIFNAIADGDIGKYPHISDAR
                     TASATFILVAASYLSAIFTSLSVRYIDSAGQLARDNHLAYRRIKGLSQTVLERVQEAV
                     VVINTEGLAVLFNRKAKDLFPALEIGRRADLSDSAAELWDQASPHTFEYVLGTLGLTA
                     RIRAVPMNKEPDKLLILYIRPQSEIQAEALSVKLAALGQLTANLAHEIRNPMSAIRHA
                     NDLLRENMEAGAADPFNAKLCKIIDGNVCRIDKMLEDISSLNKRNKTERETIGLIPFW
                     EEFKQEFLLGHPDAAGCIRLDMQGNHPTAYFDPAHLRQIMWNLANNAWRHSRKQPGSI
                     SVTIRPAQKNTVCILFADRPKCRNTCSNPFTPRRKTAPASGCMSPANWRTPISAI"
     gene            complement(11392..13695)
                     /gene="parC"
                     /locus_tag="WGQ68_001227"
     CDS             complement(11392..13695)
                     /gene="parC"
                     /locus_tag="WGQ68_001227"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEZ0715021.1"
                     /translation="MNMQPHAPHTDSNTLMLGQYAERAYLEYAMSVVKGRALPEVSDG
                     QKPVQRRILFAMRDMGLTAGAKPVKSARVVGEILGKYHPHGDSSAYEAMVRMAQDFTL
                     RYPLIDGIGNFGSRDGDGAAAMRYTEARLTPIAELLLSEIHQGTVDFVPNYDGAFDEP
                     LHLPARLPMVLLNGASGIAVGMATEIPPHNLNEVTQAAIALLKKPTLETADLMQYIPA
                     PDFAGGGQIITPADELRRIYETGKGSVRVRARYEIEKLARGQWRVIVTELPPNANSAK
                     ILAEIEEQTNPKPKAGKKQLNQDQLNTKKLMLDLIDRVRDESDGEHPVRLVFEPKSSR
                     IDTDTFINTLMAQTSLEGNVSMNLVMMGLDNRPAQKNLKTILQEWLDFRTVTVTRRLK
                     FRLNQVEKRLHILEGRLKVFLHIDEVIKVIRESDDPKADLMAAFGLTEIQAEDILEIR
                     LRQLARLEGFKLEKELNELREEQGRLNILLGDENEKRKLIIKEMQADMKQYGDARRTL
                     VEEAGRAVLTQTTADEPITLILSEKGWIRSRAGHNLDLSQTAFKEGDRLKQTLEGRTV
                     LPVVILDSLGRTYTLDAAEIPGGRGDGVPVSSLIELQNGAKPVAMLTGLPEQHYLLSS
                     SGGYGFIAKLADMVGRVKAGKVVMTTDSGETVLPPIAVYASSFINPDCKIIAATSQNR
                     ALAFPIGELKIMAKGKGLQIIGLNAGESMTHTAVSSEPEILIESEGRRGAAHKDRIPI
                     SLLEAKRGKKGKLLPISGSLKQLSSPK"
     gene            13992..14675
                     /locus_tag="WGQ68_001228"
     CDS             13992..14675
                     /locus_tag="WGQ68_001228"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEZ0715022.1"
                     /translation="MELVFIRHGQSEWNAKNLFTGWRDVKLSEQGLAEAAAAGKKLKE
                     NGYEFDIAFTSVLTRAIKTCNIVLEESDQLFVPQIKTWRLNERHYGQLQGLDKKQTAE
                     KYGDEQVRIWRRSYDTLPPLLDKDDEFSAHKDRRYAHLPADVVPDGENLKVTLERVLP
                     FWEDQIAPAILSGKRVLVAAHGNSLRALAKHIEGISDEDIMGLEIPTGQPLVYKLDDN
                     LKVLEKFYL"
     gene            complement(15488..>17425)
                     /locus_tag="WGQ68_001229"
     CDS             complement(15488..>17425)
                     /locus_tag="WGQ68_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0715023.1"
                     /translation="ISPEIFGTLFQSVMDAQERREAGAHYTETANIDKVINGLFLENL
                     RAEFEAVKALKRDKDKKLAAFYQKIQNLQFLDPACGCGNFLIVAYDRIRALEDDIIAE
                     ALKDKTGGLFDSPSVQCRLKQFHGIEIDEFAVLIARTAMWLKNHQCNIRTQIRFDGEV
                     ACHTLPLEDVAEIIHANSLRTPWQAADYIFGDPPFIGSTYQTKEQKNDLESICGHIKG
                     YGLLDYVCNWYVKAAGIMAQHPQVQTAFVSTNSICQGQQVEILWGSLLKQDIEIHFAH
                     RTFQWTSQAAGKAAVHCIIVGFRQKPQMPSEKTLYDYPDIKGEPVKHAAANINPYLID
                     APDVIIAKRSRPVHCEPDMVNGSKPTEGGNLILSTDEKEALIAAEPLAEQYIRPFVGA
                     EEFINGKTRWCLWFHGVSDVKRNHDLKQMPQVQARIQAVKTMREASSDKQTQKDAATP
                     WLFQKIRQPSDGNFLIIPRVSSESRRFVPIGYLSYETVVSDLVFTLPNASLYHFGILS
                     STMHNAFMRTVAGRLESRYRYSNTVVYNNFPFPESCRMPSENDRQDPLRAAVETAAQA
                     VLDARGQYRREAREAGLPEPTLAELYAPDAGYTALDKAHAALDKAVDKAYGYKTGKNT
                     DDEAERTAFLFELYRKAAAIA"
BASE COUNT         3637 a         4254 c         5206 g         4328 t
ORIGIN      
        1 ccaagaaaaa cacttggcta cgaaacgcca agtgttttat tcttgaatct gttccaacca
       61 ctaatacact agtgttgcac ttgaaatccg aatccaaggg cttttgaata ttgccgttgc
      121 ccgacgttct ggattcccgc aacttttcgt cattcccgcg aaagcgggaa tctagaacct
      181 ctaaattttc agataacctt tgaatattgc cgctatccca aggtctggat tcccgcctgc
      241 gcgggaatga cgggtctttt ataacctttg aatattgccg ctatcccaag gtctggattc
      301 ccgcttacgc gggaatgacg gggagggcgg atgccgtctg aagcccacct accattattc
      361 cgcttccata ccgcttggcg gcaatgtgtc cggaatgggg cggtttatcc gaaaaagccc
      421 attttgactt ggatgagttt ttccagttcg ttcaggatgc gccggcgcga gacgaaaaag
      481 atgatgtgat cgccgtcttg gatgacggtt tcggtatggt gtcccataat ggtttcgccg
      541 gttccggcgc ggacgacggc ggcaatgtgg cagccttcgg gccatttgat gccgctgatg
      601 cgcctgccga tgatggcgga agtttttttg tcgccgtgtg cgacgacttc gatggcttcc
      661 gccgtgccgc gccggatggg gtggacggca acgatgtcgc cgcgccggat gtgggcgagt
      721 atcgagccga tggtgatgag gtggggggag acgacgatgt cgattttgtt gccttcgagc
      781 aaatcgacgt agcttgagcg gttgacgatg ccgatgacgc gcttcgcgcc gaggtttttc
      841 gccaaaaggg cggacataat gttgctttcg tcgtcgttgg tcagggcgca aaatacgtcg
      901 atttcgtcga tgtattcgtt gtcgagcagg gtttcgtcgg ttgccgaacc ttgcaggacg
      961 agggtgttgt cgaggttttc ggctatccat tcggcacggc gcggccgaca ttcgatgatt
     1021 ttgacgttgt atgcgtgttc gagctgtttg gcgaggcggt agccgatgtt gccgccgccg
     1081 gcaatcatga tgcggcgggt gctggtttct ttggggcgca attcgggtat gaccgcgccg
     1141 atgttttcgg cggcggcggc aaataggatt tcgtcgcctt cgatgatgac ggtttgcggc
     1201 gcggggacga tgaggcggtt gttgcggtaa acggcgcaga tttggcagtc ggccccgtcg
     1261 ggcaaatctt gggcgatgtc ggcaatgctg cgtccgacaa gcagtccgcc gcgccgcgcc
     1321 tgtatgatga ccatccgcac gcggtcgtct gcaaaacgta aaacctgcaa tgcgcccggg
     1381 cagtctatca ggccggcaag ctgttcggta acgagctgtt cggggctgat ggtttcggtt
     1441 atgccgaata tggaaaggct gccgttttcg ttgttttcga gcttggggct gaggtattcg
     1501 aggtattcgc tggaacggac gcgcgcgatg cggccgggga tgttgaacag gtcggcggca
     1561 actttgcagg cgacgatgtt ggtttcgtcg ctgcgggaga gcgcgagcag caagtcggca
     1621 tcttccgcgc cggcgcgttc taatgtgaag ggggatgcgc cgttgccgaa aacggtttgg
     1681 acatcgaggc ggctgcctgt ttcctgcaat gctttttcgt cgatgtcgat aacggttacg
     1741 tcgttgttgg gtatggcggc aaggttttgt gcgacggtag aacctacctg tccgttgcct
     1801 aaaatgagga ttttcacggt gtgggtcgcc gagtgatgaa atggtggatt gtaccgcaac
     1861 caggggcgga aatgccgtct gaaagcagat tttgtgttca gacggcatgg cgcctcaaga
     1921 ttcgacgggg atgatgccga ttttcgcctg ccattggggc ggggcggtgg cgtggatgga
     1981 ttcgcctttg ctgtctacgg cgacggttac gggcatgtct ttgacttcaa attcgtaaat
     2041 ggcttccatg cccaattcgg ggaacgccaa gactttggaa gatttgatgg cttttgccac
     2101 gagatacgcc gcgccgccga ctgccatcag gtacacggct ttgttgtcgg cgatggcttc
     2161 gcaggtggcc gcgccgcgtt cggatttgcc gatcatgccc aagaggtcgg tttgttcgag
     2221 catttggcgg gtgaatttgt ccatgcgggt ggcggtggtg gggcctgccg ggccgacgac
     2281 ttcatcgccg accggatcga cgggaccaac gtagtaaatc aggcggttgg tgaaatcgac
     2341 gggcaattct tcgcctttgt tgagcatatc gacgaggcgt ttgtgtgcgg catcgcggcc
     2401 ggtgaggatt ttgccgttca acagcaatac gtcgccggtt ttccagcttg ccacttcttc
     2461 tttggtcagc ttgtcgacat cgacgcgttt gccgttgtcg gggctgtaag tcaaatcggg
     2521 ccagtcttcg acgcgcggcg gcgtgagttc gacagggcct gagccgtcca attcgaactc
     2581 gacgtggcgg gtggcggcgc agttcggaat catggcaatc ggtttggagg cggcatgggt
     2641 cggataatcg aggattttca cgtccaacac ggtggtcagt ccgcccaagc cttgtgcgcc
     2701 gatgcccagc gcgttgactt tttcaaagag ttcgaggcgc agggcttcgg tggtggataa
     2761 ttctgcgccg gacgcggctt tttcctgcaa ttcttggatg tcgatgtggc tcatcaggga
     2821 ctcttttgcc atcaacacgg ctttttcagg cgtgccgccg atgccgatgc ccaagatgcc
     2881 gggaggacac cagcccgcgc ccatcgtcgg aatggttttc aatacccaat cgacgatgtt
     2941 gtcggaaggg ttgagcatgg cgagtttgga tttgttttca gaaccgccgc cttttgccgc
     3001 gcaggttact tcgactttac cgcccggcac gatgctcatg tggatgacgg cgggcgtgtt
     3061 gtctttggtg ttttgacgtt tgccggccgg atcggcgagg acagaggcgc gcagggtgtt
     3121 gccttcccaa gtgtaggcgc ggcgcacgcc ttcgttaacc atttcttcca cgctcatgtc
     3181 cgcatcccac tgcacatcca taccgacttt caaaaacacg gttgcgatac ctgtgtcttg
     3241 gcagatgggg cggttgtttt cggcacacat gcggctgttg accaaaatct gcgtcatcgc
     3301 gtctttggcg gcggggtttt cttccttctg ccacgcttta tagagcgcgt cgatgtagtc
     3361 tttgggatgg tagtagctga tgaattggaa ggcatcgcaa atgctttgga taaagtcttc
     3421 ctgtttgatg acggtcatga ttgtgttcct tttcaaggtt gtggcggtaa gtgtctgctg
     3481 ctgttttaag caaccttttt gaaggctgtt ttttatcagg ttgaaatagt gccagcgttt
     3541 gtacggtaaa gacaatacat aattctaatc tcttgattat caatttgttt taataaaata
     3601 ctgttttact cggattttga tatttgtaat ctttgcctgt tgctttgcgg tagggagaaa
     3661 tcagcaggca gttcattgta ggccgattga tgaacaatgc cgtctgaaag ggcttcagac
     3721 ggcattgtgt tcggatgggc gggcttattt tgcggcctgt ccgttttctt ttgcaaaata
     3781 tttggcgtag attttgtcgt attcgccgct ttcgcgtact tttttcaacg catcgttcag
     3841 cattttgacg gttgcttcgt cgcctttgcg taccgcgatg ccgtagtgtt cggtggtgaa
     3901 gtcgggcagg gtaacgaagt ccattccttt ggtcggattg tttttcacat aattggcgat
     3961 gaccgcgctg tcgctgacca cggaatccaa gccgccgttt tccagttctt tgataatcag
     4021 gggaacgttt tcaaagcgcg cgattttcgg gttgtcgttg cccaagagtt tggaaacgga
     4081 gaaatcgccc gtgtagccgg ttaccacgcc gactttgttc atgtttttca aatcgtcgga
     4141 agaagatatt tttttgcctt tcggaacgag gacgacttgg gtgatttcaa aatacgggtc
     4201 gctgaagtcc atagactgtt tgcggtcgtc ggtaatggtt acgcccgaca tcacaacgtc
     4261 cgcatcgccg ttgttcaagg cggggaaaag gctgtcccac ggctggtgtt tgaattcgat
     4321 tttaaaattg cccgccttcg ccatcgcgtt catcaaatcc acatcgaaac cttcgacatt
     4381 gcctttcgag tctaaagatt caaagggggc aaactcggcg ttggaagcca cgcggtacac
     4441 tttgtcgggg tttgcggcgg gcgcggcggc atctttgccc tgaccgccgc aggcagacag
     4501 cgcgagcgcg gaacaggcaa gggcggcggc aatccatttt ttcatattca tcaaaattcc
     4561 tttcggtaat caaaaagcct aaaaaatcag tttaacataa aattaacgtt ctttttcacg
     4621 gaaaaacgca taagtaacgt cgtggaacaa atccgtatgc gggctgtcct catagctcaa
     4681 ttctgccaag gcgtcctcaa aagcaagctg gatggactcg accgcgactt cggcggtgtc
     4741 ggcgggaagg gcgcggatta gggctttgaa ggcggtggac aggacgtggt tctgcatacg
     4801 cagcacgtcg ttcgcctctt ccagatattc caaacgctgt gcgatgctgc ccatgatgta
     4861 tcctcgcgaa tctgttaaaa tgcgccatta tatcaaaccg cgctttgggc gcgggtttgt
     4921 ctgcttgcaa agattaagaa ccggaagccg aaaatggatg cgaataaaga gccgaattgg
     4981 gaaaaacgtt tggaaaacct gcgtcagacg cgccgtatga aggcagtgcc gacttacgcg
     5041 ccgctgtccc gcccctctga aaccggaacg gacgcgcctg tgccgaaact ggacgatgag
     5101 agccgagccc gtgtgttgaa ggcgtatctg aaaaaatggc aggaggaaca caatgccgcc
     5161 gttgccgatt gcggcgagga agtcgaaacc gacccgatga ttgtccttca ggaaaactgg
     5221 ctcaacgcgc aggcttccct gcaaatgtcg gtttcggaaa aacacatcga atcgcggcgg
     5281 agaatcctgt tcgatgccaa aaaagacagt gccgaatttg aaaccggaca gattgaaggc
     5341 ggggaaaacg ccgatgcggc agaatcgggt acggcggcag acggggttgc ggaaaacggc
     5401 gaaggcggtg cggaagaggc gttgatgccc gtccaaatca atatcctgaa tccgaaagcc
     5461 gtcaaccggc gcgaagtgtt ctgcctgtcc gaacaggagc tgaccgaacg gctgattaaa
     5521 cgtctgcgcc cgcatctgac cgataccgtc aacggcatca tccgtacggc ggtgcaaaag
     5581 cagatggcgg tttttaccta tcagctccag cagatgctgc acgagcaggc gggtgcggta
     5641 gtcgaagacg tgttggaaca cgatgtccgc aaaatcctca acgacatcaa gtacgagtta
     5701 aaatacaaac gctgattgcc atgccgtctg aaagccgttt cagacggcat ttgcattctc
     5761 atccaacttg aaagcctcat agaacaggcg caaaatatta atatagtgga ttaacaaaaa
     5821 tcaggacaag gcgacgaagc tgaagacagt acaaatagta cggaaccgat tcacttggtg
     5881 cttcagcacc ttagagaatc gttctctttg agttaaggcg aggcaacgct gtaccggttt
     5941 ttgttaatcc actataaatt aaacaacggg cgtgcgctcc gttgccgaac ggacaaaacc
     6001 gaggaatcac cgatgagcaa aaaactccat ccgcaaacgc tcgccatacg cggcggcaaa
     6061 gaacaaaccg aataccgcga acacaatcag gcattgtttc tgaccagcag cttcatgtgg
     6121 gacaacgccc aacacgctgc cgatttgttt tcaaaaaaaa tcaaaggctt cacttatacc
     6181 cgtaccgcca acccgaccac agccgccttt gaaaaacgca tcgccgcttt ggaaggtgcg
     6241 gaacgcgcgg tcgccacttc gaccggcatg tccgcgattc aggcggcgtt tttcaccttt
     6301 ttgcaggcgg gcgaccatgt gatttccagc cgcagcctgt tcggcacgac cgtcggcttt
     6361 atcaataaca tcgttaccaa attcggcatc gaagtgagtc acgtgtcgcc gactgatata
     6421 aacgaatgga aagccgccgt caaagccaac accaaactgc tgtttttgga aacgccgtcc
     6481 aatcccttgg gcgaagtggc cgacttggaa gccttggcgg aattggcgca cggcatcggc
     6541 gcgctcttgg tggtggacaa cagcctgctg tcgcctgtcg gctctcagcc tttgaaacac
     6601 ggcgcggata tttccgtttc ctccgcgacc aaagccatag acggacacgg gcgcgtgatg
     6661 ggcggcgtgt tggcgggttc ggaagaactg atggcgcagg tcgccgttta ctgcaactct
     6721 tgcgggctgg caatgtcgcc gtttaacgca tggcagttgt tgagcggcgt ggaaaccctg
     6781 tctctgcgta tggaaaaaca gttcgacaac gccttgaaaa tcgcgcaatg gctgcaagcg
     6841 cagccgcagg ttcaagccgt gtattacacc ggattacccg accatcctca ggcggagctt
     6901 atccgcaaac agcaaaacgg cggcggcatc gtcatcggct ttgaagtcgc cgaccaagca
     6961 gccgcgtgga aagtcgtgga cggcgtcgaa cttttctccc gtaccgccaa cctcggcgac
     7021 gtgcgctcga ccatcaccca tccgtggaca accacgcacg gcaggatgca gcccgaagaa
     7081 aaactcgccg ccaacatccg ccccggattg gtgcgcctgt cggtcggttt ggaatatgtt
     7141 ggcgatttga ttgacgattt gaaacaggca ctcgcgcgct gattgcgtgt tcagacggca
     7201 tcggcggaaa aacaccgcaa atgccgtctg aaacccttat cttatatggc atacacgtca
     7261 acaataggaa aaaaatatgg atgaagcggt ttttgccgat tgggcgttga aaatctgcct
     7321 gaccggtttg attatttttt tgggttttat cgtttggaat ttgggcaagg agtctaaagc
     7381 gggcaaattc ggcatcgcca ttttgttttt ggttttgggc ttgggcgtgt tcggttttgt
     7441 gttcaaagag ttattgatta agtttttggt attgccgaaa taggtttgcc gtctgtcaaa
     7501 atgctgtctg aaatcataaa aagtaatctg caccctaaaa gtttgattta accgccaact
     7561 gattaaggtg cggattttta tgacaaaata aaaatcatct ttccgcagtc attcccacgg
     7621 acgcgaaagt ctgtcttttt gaatcccgga taattttaca tcgtcattcc cgcgcaggcg
     7681 ggaatctagt ccgttcggtt tcagtcattt acgataaatt cctgttgctt ttcatttctg
     7741 gattcccact tccgtgggaa tgacggcgga gcggataaat tctcgcaatc taaaacctcg
     7801 tcatttccat aaaaaacagc aacccgaaac agcaacttaa aaacagcaac ctgaaacttc
     7861 gtcattcccg cgtaggcggg aatctagtcc gttcggtttc ggtcatttac gataaattcc
     7921 tattgctttt catttcctga ttcccacttc cgtgggaatg acggcggagc ggataaattc
     7981 tcgcaatcta aaacctcgtc atttccataa aaaacagcaa cccgaaacag caacctgaaa
     8041 cctcgtcatt cccgcgcagg cgggaatcca gatcattggg tagcggcaat gttcaaaagt
     8101 cgtctgaaaa atcagaagtt ctagattccc gtttttacgg gaatgacgga atttcagacg
     8161 gcatccgccc gccccgtcat tcccgcgcag gcgggaatct agtccgttcg gtttcggtca
     8221 tttacgataa attcctgttg cctttcattt ctagattccc acttccgtgg gaatgacggt
     8281 ttggaagttg cctgaaactc gaaaacagct atagtggttt aaatttaaac cactataaaa
     8341 ctgaatgaat cggattccca cttccgtggg aatgacaaac tttaaggtgt gatgatttat
     8401 ccaaaacagt cgaaacgcaa aaaccggttt ctcgtttgca taagaacggc aaatttttcg
     8461 gtgtcttgtt ttatgggcgt tatcccttgt ccgtcccgta gggcaggtaa acgtcgaagc
     8521 gggtgctttt gccggtgtat tggtaggcag gggcttgtcc ggcaaggtgt tcgccgaggc
     8581 gggggcgttt gacgacgacg cgtttttttg ctgtttggcg tgcggtatgc aggagtgccg
     8641 cttcatcttg cgcttcgccg acgagccgat ggaagtaggt catttctttt ttaacggcgg
     8701 cacttttgcg gcgttcggga tacatggggt cgagatagac gatgtcgggt ttgccttgtg
     8761 tttgaacaag ggcgggcatt tgttcggcgg cgttgccgaa atggaggttg atgtgcgcgg
     8821 cggtgtcttg cgtttcggga ttgaggaggg cgcggcggat gccgtctgaa agcaggcagg
     8881 cgacggcggg atgttgctcg aaggcggtaa cggccagccc gagcgaggcg aggacgaagc
     8941 tgtcgcgccc caatcctgcg gttgcgtccc aaacggtggg gtgcgcggtg tggttgacgg
     9001 ctttggcgat gagttcgccc ccgccttttg tgcgccggta ttgtgccgcg ccggaggcaa
     9061 aatcgacgat gacgctgctt ttttccccga caagcctgag gctgacggta tcgtgttcgg
     9121 caaggaggta actgccttgt tcgggcggtt gggaaacggg cacaaggggg aatgcccgta
     9181 tcagggtgcg gacggtttcg gtggcggtgt tgtcaataag gatgtcggtc atggtgtttt
     9241 gtgtccgttc gggcgtgggg cgaacccgct ttcccgtgcc gtttcagacg gcattttttc
     9301 gggtgggcgg agatggtgcg gatgccgttt tgtcgtcagc cgatgttgag gcgttccata
     9361 cggtagcgca tggaacggaa gctgatgccc aagcgtttgg cggcctgcgt gcggttgcct
     9421 tcggtttgtt tgagggtttg ttcgatgatg tcgcgttcga tttggtcgag atagtcctgt
     9481 atctgcatgg tatcggggtc gaacgcgccg caacgcaacg cttcctgtgc ctgatcggcg
     9541 ttgccgaagg cggtgatgac ggcaaccggc gtatcgagca ggcggctgtt gatgtgttgg
     9601 acgacttcca gccccgagct gtccggcata cgcatatcgg tcaggacgag cgaatagcgt
     9661 tggctgtcga gcttgttttt ggcttcggca acgcctgacg cggtatggac gcgcaagccc
     9721 attttcatca gggtcatttc catcaggtcg agaatgtcgg tttcgtcatc gacgacgagg
     9781 acggggtgtt gcagttcagt cattggtttt ttccggtaat gtgagttcga aacatttggc
     9841 ttccggtagg taggtcaaat cgccgaaatt ggcgtgcgcc agttcgcggg cgacatacag
     9901 cccgaggccg gtgccgtttt ccgccgtggt gtaaaagggt tcgaacaggt gttcctgcac
     9961 ttcgggcggt cggcaaagag gatgcagacg gtgttttttt gcgcggggcg gatggtgacg
    10021 gaaatcgagc cgggctgttt gcggctgtgc cgccacgcgt tgttggcgag attccacata
    10081 atctgccgca ggtgggcggg atcgaaatag gcggtcgggt gattgccctg catatccaag
    10141 cggatgcagc cggcggcatc gggatggccg agcaggaact cttgtttgaa ttcttcccaa
    10201 aacggtatca ggccgatggt ttcgcgttcg gttttgttgc gcttgttgag tgaggaaatg
    10261 tcttcgagca ttttgtcgat gcggcagacg ttgccgtcga tgattttgca caatttggcg
    10321 ttgaacggat ctgccgcccc cgcttccata ttttcgcgca gcaggtcgtt ggcgtggcgg
    10381 atggcggaca tcgggttgcg gatttcgtgg gcaaggttgg cggtcagttg tccgagcgcg
    10441 gcaagtttga cggacagggc ttctgcctga atttcgcttt gcgggcggat gtagaggata
    10501 agcagcttgt ccggctcttt gttcatcggc acggcgcgga tgcgggcggt caggccgagt
    10561 gtgccgagga cgtattcgaa agtgtgcgga gaggcttggt cccacagttc ggcggcagaa
    10621 tcggacaggt cggcgcgccg tccgatttcg agtgcgggga aaaggtcttt cgccttccgg
    10681 ttgaacagca ccgccagccc ttcggtattg atgacgacga cggcttcttg aacgcgttcg
    10741 agtacggttt ggctcaagcc tttgatgcgc cggtaggcga ggtggttgtc gcgggccagt
    10801 tggccggcgc tgtcgatgta tctgaccgac agtgaggtga agatggcgga aagataggag
    10861 gcggcgacga ggatgaaggt tgccgaggcg gttcgggcat ccgatatgtg cggatatttg
    10921 ccgatatcgc cgtcggcaat ggcgttgaat atcagcagga tggcggcgta gctggcatag
    10981 agcagggggt aacgcccgta gctgagcagg caggagcaga cgacgaaggg cagaatcagg
    11041 atgccgaagc cggaatcgat gccgccgaac aggtaggtca gcacgccgat catcgtgatg
    11101 tccgccaccg cgctgaaacg cggcattttc agcgactgcc attgccaatg cggatggatg
    11161 atggaaaaga aaatcagcca gcaggaaatg gcgagccaaa cataaaatgc ccacgccgtc
    11221 cacgaagcgt gcaggttttc atgtccgctg acctccaacc cgaagatatg cataatcagc
    11281 agggggaaga cgatggcgat gcgggcgatg ttgatcagat tgggaatccg gtctttgagt
    11341 ttctcaagtt cgcgggtatt ggaaattacc atagtatgtg cagagtcggg tttatttcgg
    11401 ggaagaaagc tgtttcaggc tgcccgatat gggtaatagt ttgccttttt tgccgcgttt
    11461 tgcctcaagc agggagatgg ggatgcggtc tttgtgcgct gcgccgcgcc tgccttcgct
    11521 ttcaatcagg atttccggct cggaagaaac ggcggtatgc gtcatcgatt cgccggcgtt
    11581 taatccgatg atttgcagcc ctttgccttt cgccataatt ttcaattcgc cgatggggaa
    11641 ggcgagggcg cggttttgac tggtggcggc aatgattttg cagtcggggt tgatgaacga
    11701 ggaggcatag acggcaatcg gcggcaggac ggtttcgcca ctgtctgtgg tcatcaccac
    11761 tttgcccgct tttacgcgtc cgaccatatc agccaacttg gcgatgaagc cgtagccgcc
    11821 gctgcttgat aataaataat gttgttccgg caatcctgtc aacatcgcaa cgggtttcgc
    11881 gccgttttgc aactcgatca aggatgaaac cggcacgccg tcgccgcgtc cgccgggaat
    11941 ttcggcggca tcgagtgtat aggttctgcc caatgaatcg aggatgacga cgggtaaaac
    12001 cgttctgcct tcaagggttt gtttgaggcg gtcgccttct ttgaacgcgg tttggctcaa
    12061 atcgagattg tgtccggcac ggctgcgtat ccagcctttt tccgacagaa tcagtgtgat
    12121 gggttcgtcg gcggtggttt gtgtcagcac ggcgcgtccg gcctcttcca ccagcgtgcg
    12181 gcgtgcatcg ccgtattgtt tcatatccgc ctgcatctct ttgataatca gcttgcgctt
    12241 ttcgttttca tcgcccaaaa ggatgttcag acggccttgt tcttcgcgca attcgttcaa
    12301 ttctttttcg agtttgaaac cttccaaacg tgccaactgg cgcaggcgga tttccaaaat
    12361 gtcttcggct tggatttcgg tcagcccgaa cgccgccatc aaatcggctt tcgggtcatc
    12421 tgattcgcgg atgactttaa tcacttcgtc gatgtgcaga aagactttca gacggccttc
    12481 gaggatgtgc agccgttttt ccacttggtt caaacggaat ttcagacggc gtgttacggt
    12541 tacggtgcgg aaatccagcc attcctgcaa aatcgttttc aggtttttct gcgcggggcg
    12601 gttgtccagc cccatcatca ccaagttcat ggacacattg ccttccagcg aagtttgcgc
    12661 catcagcgtg ttgatgaagg tatcggtatc gatgcggctg gatttcggtt cgaacaccag
    12721 tcgcacggga tgttcgccgt cggactcgtc gcgcacgcgg tcgattaaat ccagcatcag
    12781 ctttttggta ttgagctggt cttggttgag ctgctttttg cccgctttcg gtttcgggtt
    12841 ggtctgctct tcgatttcgg caaggatttt ggcggaattg gcgttcggcg gtagctcggt
    12901 tacgatgacg cgccattgtc cgcgcgccaa tttttcgatt tcataacgcg cacgcacgcg
    12961 cacgctgccc ttgccggttt cataaatccg gcgcaattcg tccgccggcg tgatgatttg
    13021 accgccgccg gcaaaatcgg gcgcaggaat atattgcatc aggtcggcgg tttccagcgt
    13081 cggctttttc aacagcgcaa tcgctgcctg cgtaacttcg ttcaaattgt gcggcggaat
    13141 ttcggtcgcc atacctaccg cgatgcctga cgcgccgttg agcaacacca taggcagacg
    13201 tgcaggcagg tgcagcggtt cgtcaaacgc gccgtcgtag ttcggcacga aatccaccgt
    13261 cccctgatgg atttcggaca acagcaattc cgcaatcggc gtcagccgcg cttcggtgta
    13321 acgcatcgcc gccgcaccgt cgccgtcgcg cgaaccgaag ttgccgatgc cgtcgattaa
    13381 gggatagcgc aaggtaaaat cctgcgccat ccgcaccatc gcctcatagg cggaactgtc
    13441 gccgtgcggg tggtatttac ccaaaatctc gccgaccacg cgcgccgatt tcaccggctt
    13501 cgcccccgcc gtcaaaccca tatcgcgcat ggcaaacaaa atgcgccgct gcacgggctt
    13561 ctggccgtct gaaacttcag gtagcgcgcg gcctttgacc acgctcatgg cgtattcgag
    13621 ataggcgcgt tcggcgtatt gaccgagcat cagcgtattg gaatcggtat gtggagcgtg
    13681 cggttgcata tttatcgtat ttccttttcg gttgaaaccc cgccacttgg acatctgtcc
    13741 ttcggggcgg tagaatcaga ctttatttgg gagggggcaa ccccttccaa atcagggcaa
    13801 cacatagggc ggtgctttat gtgtcgtcct gtgtgttgaa acattgtgta tgcggaatgt
    13861 aaagcaaagg acattattgt aaatcaaaac ggcaaaccat agtgggcgcg tgtctgaatc
    13921 tgaatcaaat acgggaaatg tgaaaatatg ttataaataa ggctttccca ctttcacaca
    13981 ttggagacga tatggaactg gtatttatcc gccacggaca aagcgaatgg aacgcgaaaa
    14041 acctgtttac aggctggcgc gacgtgaagc tgagcgagca ggggcttgcc gaggctgccg
    14101 ccgccggtaa aaaactgaaa gaaaacggct atgagttcga catcgccttc acatccgtcc
    14161 tgacccgcgc gattaagacc tgcaacatcg ttttggaaga atccgaccaa ctgttcgtac
    14221 cgcaaatcaa aacgtggcgg ctgaacgaac gccactacgg ccaactgcaa ggcctggaca
    14281 aaaaacaaac cgccgaaaaa tacggcgacg agcaagtccg catctggcgg cgcagctacg
    14341 acaccctgcc gccgcttttg gacaaagacg atgagttttc cgcacacaaa gaccgccgct
    14401 atgcccatct gcctgccgat gtcgtacccg acggcgaaaa cctgaaagta acgctggagc
    14461 gcgtattgcc gttttgggaa gaccaaatcg cccccgcgat tttgagcggc aaacgcgtct
    14521 tggtggcggc gcacggcaac tccctgcgcg cgctggcaaa acacatcgag ggcatttccg
    14581 acgaagacat tatgggcttg gaaatcccga ccggtcagcc gctggtgtac aaattggatg
    14641 acaacctgaa agtcctcgaa aaattctacc tgtaagggtt tgaaataaaa aatgccgtct
    14701 gaaggcttga gcgttcagac ggcatttttg acggtaaacg tagcaactgc tttcgcgaga
    14761 acgacgagat tttaggtttt tgtttttctg ttttcgtggg aaggatgggt tttaggttgc
    14821 ggtcatttat cggaaaagca gaaaccgctc cgccgtcatt cccgcgcagg cgggaatcta
    14881 gaacctctga attttcagac aacctttgaa tattgccgcc gtcccgcgtt ctggattccc
    14941 gcctgcgcgg gaatgacgat ttataagttc ccgaaacgcc cgaaaaaacc gaaatccgac
    15001 aggcaggatt ttcgctttcg tggggatgac gggatttttg taaccgtagc aactgctttt
    15061 gcggtaatga cgggatttta agttacggta agttatcgga aagacagaaa cttctccgcc
    15121 gtcattcccg cgaaggcggg aatctgaaca cgtccgtagg gaaacctata tcccgtcatt
    15181 cccatgaaag tgggaatcca ggatgcaggg gaaatcgttt tatccgataa gtttccgccc
    15241 cgaaaggtct ggattcccgc ctgcgcggga atgacgggat ttttgtaacc gcagggtggg
    15301 cttcagcctg ccaatcaaca accgaaaatg aagaaaaacg gtgggctgaa gcccacccta
    15361 cgcaattgct tttgcggaga tgacggggtt ttagggtgaa gatggtggga gttggtggga
    15421 gttggtgggc tgaagcccac cctacgcaac tatcctacgc aactactacg caactactac
    15481 gcaactacta cgcaattgcc gccgccttgc ggtacagctc gaacaggaag gcggtgcgtt
    15541 cggcttcgtc gtcggtgttt ttgcctgttt tgtagccgta ggctttatcg actgccttgt
    15601 cgagggcggc gtgggctttg tctagggcgg tatagcctgc gtcgggcgca tagagttcgg
    15661 cgagggtcgg ctcgggcaaa ccggcttccc gcgcttctcg gcggtattgt ccgcgcgcgt
    15721 cgaggacggc ttgggcggcg gtttcgacgg cggcgcggag cgggtcttgg cggtcgtttt
    15781 cagacggcat ccggcagctt tcggggaagg ggaagttgtt gtacacaacg gtattggaat
    15841 agcggtaacg actttccaga cggccggcaa cggtacgcat aaaggcgttg tgcatggtgg
    15901 agctgaggat gccgaagtgg tagaggctgg cgtttggaag ggtaaatacc aaatcgctga
    15961 caactgtttc atatgataga taaccgatgg ggacgaaacg gcggctttcg gatgagacgc
    16021 gtgggataat cagaaaattg ccgtctgaag gctggcggat tttttgaaaa agccacgggg
    16081 ttgctgcatc tttttgagtt tgtttgtcgc tgctggcttc gcgcatggtt ttgactgcct
    16141 gaatacgggc ttgaacttgg ggcatctgtt tcaggtcgtg gttgcgttta acgtcggata
    16201 cgccgtgaaa ccacaggcac caacgggttt tgccgttgat aaattcctct gcgccgacaa
    16261 agggacggat gtattgttcc gccaagggtt cggcggcaat cagggcttct ttttcatcgg
    16321 ttgaaaggat aaggttgccg ccttcggtcg gtttgcttcc gttgaccatg tcaggttcgc
    16381 aatgtacggg acggctgcgc ttggcaataa tcacatcggg cgcgtcaatc agatacggat
    16441 tgatgttggc cgcggcgtgt tttacgggtt cgcctttgat gtcgggatag tcgtagaggg
    16501 ttttttcaga cggcatttgc ggcttttggc ggaagccgac gatgatgcag tggacggcgg
    16561 ctttgcctgc ggcttggctc gtccattgaa aggtgcggtg ggcaaagtgg atttcgatgt
    16621 cttgcttcaa aaggctgccc caaaggattt cgacctgctg gccttggcag atggaattgg
    16681 tggaaacaaa tgccgtctga acttggggat gctgcgccat gataccggcg gctttgacat
    16741 accagttgca gacgtaatcc aacaggccgt agcctttgat atggccgcag atgctttcga
    16801 ggtcgttttt ctgctctttg gtttggtagg tcgagccgat aaagggcgga tcgccgaaga
    16861 tgtagtccgc cgcctgccaa ggtgtgcgga ggctgttggc gtggatgatt tcggcgacgt
    16921 cttcgagcgg cagcgtatgg caggcgactt cgccgtcgaa gcggatttgc gtgcggatgt
    16981 tgcattggtg gtttttgagc cacatggcgg tgcgggcgat gaggacggca aattcgtcta
    17041 tttcgatgcc gtgaaattgt ttcagacggc attggacgga cgggctgtcg aataagccgc
    17101 ctgttttgtc tttgagcgct tcggcgatga tgtcgtcttc gagggcgcgg atacggtcgt
    17161 aggcgacgat aaggaagttg ccgcagccgc aggccgggtc gaggaattgc aggttttgga
    17221 ttttttggta gaaggcggcg agttttttgt ctttgtcgcg tttgagggct ttgacggctt
    17281 caaattcggc gcgcaggttt tctaaaaaaa ggccgttgat gactttgtcg atgttggcgg
    17341 tttcggtgta gtgtgcgccc gcttcgcgcc gctcttgtgc gtccatgact gattggaaga
    17401 gcgtgccgaa gatttcggga ctgat
//
LOCUS       DAWFHY010000043        17180 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.043, whole genome shotgun sequence.
ACCESSION   DAWFHY010000043 DAWFHY010000000
VERSION     DAWFHY010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 17180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.043"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(77..730)
                     /locus_tag="WGQ68_001230"
     CDS             complement(77..730)
                     /locus_tag="WGQ68_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01621 family pseudouridine synthase"
                     /protein_id="HEZ0715024.1"
                     /translation="MLEILFRHQDFVAINKPGGISVHQDSGETGLARTLAIQLGVERV
                     WLLHRLDKQTSGILLFALNKESASALSGQFAGKSIKKTYLALSDRKPSKKQGWIKGGM
                     EKSRRGMWKLTRNTENIAVTRFHSISIAEKLRLFILEPHTGKTHQLRVAMKSLGSPIL
                     GDSLYGGTESETMFLYAWRIQFAYQNRQIEIVAPLKNEWQTENISHALEEFYMAKAG"
     gene            complement(762..1274)
                     /gene="coaD"
                     /locus_tag="WGQ68_001231"
     CDS             complement(762..1274)
                     /gene="coaD"
                     /locus_tag="WGQ68_001231"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEZ0715025.1"
                     /translation="MLPNTPRRAVYAGSFDPPTLGHLWMIRQAQSMFDELIVAIGINP
                     DKRSTYTVAERQDMLCDITKMFPNVRTDVFENRFLVHYAREVDAGFIVRGIRSASDYE
                     YERSMRHINSDLAPEISTVFLMPPREIAEVSSTMVKGLVGPEGWTETIHRYVPQAVYE
                     KILAEHQHEN"
     gene            complement(1398..2081)
                     /locus_tag="WGQ68_001232"
     CDS             complement(1398..2081)
                     /locus_tag="WGQ68_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HEZ0715026.1"
                     /translation="MQHDVYDYTAHTVSKNTVLQKTYRLLGFSFIPAAAGAALAANAG
                     FNFYAAFGSRWIGFAVVLAFFYGMIHFIEKNRYSNTGVTLLMVFTFGMGVLIGPVLQY
                     ALHIADGAKIVGIAAAMTAAVFLTMSALARRTRLDMNALGRFLTVGAVILMVAVVANL
                     FLGIPALALTISAGFVLFSSLMIMWQVRTVIDGGEDSHISAALTLFISLYNIFSSLLN
                     ILLSLNGED"
     gene            complement(2238..2504)
                     /locus_tag="WGQ68_001233"
     CDS             complement(2238..2504)
                     /locus_tag="WGQ68_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HEZ0715027.1"
                     /translation="MARMVFCVKLNKEAEGMKFPPLPNELGKRIFENVSQEAWAAWTR
                     HQTMLINENRLSLADPRAREYLAQQMEQYFFGDGADAVQGYVPQ"
     gene            complement(2577..3047)
                     /gene="rlmH"
                     /locus_tag="WGQ68_001234"
     CDS             complement(2577..3047)
                     /gene="rlmH"
                     /locus_tag="WGQ68_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEZ0715028.1"
                     /translation="MNITVLAVGTKMPRWVDEAVAEYAKRFGRDVAYALKEIKPEKRG
                     AGVNAAQGMAAEEKRILEAVPQGAFLVVLDERGKAPTSVELAEHLKSWRQNGEHVCFV
                     IGGADGMTDRLKQQARIMMRLSSLTLPHGMVRVLLTEQLYRAASILHNHPYHRE"
     gene            complement(3101..3487)
                     /gene="rsfS"
                     /locus_tag="WGQ68_001235"
     CDS             complement(3101..3487)
                     /gene="rsfS"
                     /locus_tag="WGQ68_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEZ0715029.1"
                     /translation="MNEQELQDLQKMVGVAVNALEDIKAKDISVLETQDKTSLFARMI
                     IASGDSTRQVKALANNVAVDLKEAGFEILSTEGDSGEWTLVDAGDLVVHVMLPAVRDF
                     YDIDTLWGGEKPSFHAGMQKPWHAAD"
     gene            complement(3546..4151)
                     /gene="nadD"
                     /locus_tag="WGQ68_001236"
     CDS             complement(3546..4151)
                     /gene="nadD"
                     /locus_tag="WGQ68_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HEZ0715030.1"
                     /translation="MKKIGLFGGTFDPIHNGHLHIARAFADEIGLDAVVFLPTGGPYH
                     KDAASASAADRLAMVELATAEDARFAVSDCDIVRRGETYTFDTVQIFRQQFPSAQLWW
                     LMGSDSLMKLHTWKKWQMLVRETNIAVAMRQGDSLHQTPRELHAWLGNALQDGSVRIL
                     SAPMHNVSSTEIRRNLAGQGVSDGIPPAAARYIREHGLYEK"
     gene            complement(4148..4747)
                     /locus_tag="WGQ68_001237"
     CDS             complement(4148..4747)
                     /locus_tag="WGQ68_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEZ0715031.1"
                     /translation="MSELDNILAHNRQFVESGEYEKYFTDKYPERGLAVLSCMDARII
                     GLLPDALGLKNGDAKLIKNAGALVTHPWGSVMRSLLVAVFELKVREIMVIAHHDCGMQ
                     GLNAEEFLGRVRESQIPEDRIETLRYAGIDLDGWLTGFDNVEDSVRHTVDLIRNHPLM
                     PRHIAVHGLVIHPVTGKLTLVVDGSVSDGMDLSEGMETS"
     gene            complement(4776..6317)
                     /locus_tag="WGQ68_001238"
     CDS             complement(4776..6317)
                     /locus_tag="WGQ68_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEZ0715032.1"
                     /translation="MDGRRWAVWGAFALLPSAFLAVMVVAPLWAVAAYDGLAWRAVLS
                     DAYMLKRLAWTVFQAAATCVLVLPLGVPVAWVLARLAFPGRALVLRLLMLPFVMPTLV
                     AGVGVLALFGADGLLWRGRQDTPYLLLYGNVFFNLPVLVRAAYQGFVQVPAARLQTAR
                     TLGAGAWRRFWDIEMPVLRPWLAGGVCLVFLYCFSGFGLALLLGGSRYATVEVEIYQL
                     VMFELDMAVASVLVWLVLGVTAAAGLLYAWFGRRAVSDKAVSPVMPSPPQSVGEYVLL
                     AFAAAVLSVCCLFPLLAIVVKAWSAGESWRVLMESETWQAVWNTLRFSAAAVYAAAVL
                     GVVYAAAARRSAWMRGLMFLPFMVSPVCVSAGVLLLYPQWTASLPLLLAMYALLAYPF
                     VAKDVLSAWDALPPDYGRAAAGLGANGFQTACRITFPLLKPALRRGLTLAAATCVGEF
                     AATLFLSRPEWQTLTTLIYAYLGRAGEDNYARAMVLTLLLAAFALGIFLLLDGGEGGK
                     RTETL"
     gene            6557..7942
                     /locus_tag="WGQ68_001239"
     CDS             6557..7942
                     /locus_tag="WGQ68_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HEZ0715033.1"
                     /translation="MDGWTQTLSAQTLLGISAAAIILILILIVKFRIHALLTLVIVSL
                     LTALATGLPTGSIVNDVLVKNFGGTLGGVALLVGLGAMLGRLVETSGGAQSLADALIR
                     MFGEKRAPFALGVASLIFGFPIFFDAGLIVMLPIVFATARRMKQDVLPFALASIGAFS
                     VMHVFLPPHPGPIAASEFYGANIGQVLILGLPTAFITWYFSGYMLGKVLGRTIHVPVP
                     ELLSGGTQDNDLPKEPAKAGTVVAIMLIPMLLIFLNTGVSALISEKLVSADETWVQTA
                     KIIGSTPIALLISVLVALFVLGRKRGESGSALEKTVDGALAPVCSVILITGAGGMFGG
                     VLRASGIGKALADSMADLGIPVLLGCFLVALALRIAQGSATVALTTAAALMAPAVAAA
                     GFTDWQLACIVLATAAGSVGCSHFNDSGFWLVGRLLDMDVPTTLKTWTVNQTLIALIG
                     FALSALLFAIV"
     gene            7962..8480
                     /locus_tag="WGQ68_001240"
     CDS             7962..8480
                     /locus_tag="WGQ68_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEZ0715034.1"
                     /translation="MTTHFVVMGVCGCGKTTAALSLQKHLGQCPYAEGDEFHTQANRD
                     KMGAGIPLTDEDRYPWLGNLRDWMTQQAQNGANHTIVTCSALKRGYRDILRGAEGKAA
                     FIHLSPPQDINLERMMSRKGHYMKAGMLDSQLEILEELGEGEYGVKIANPGTPEAVEA
                     DILNWVASENLL"
     gene            complement(8819..9736)
                     /gene="thrB"
                     /locus_tag="WGQ68_001241"
     CDS             complement(8819..9736)
                     /gene="thrB"
                     /locus_tag="WGQ68_001241"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEZ0715035.1"
                     /translation="MSVYTSVSDDEMRGFLSGYDLGEFVSLQGIAQGITNSNYFLTTT
                     SGRYVLTVFEVLKQEELPFFLELNRHLSMKGVAVAAPVARKDGRLDSVLAGKPACLVA
                     CLKGSDTALPTAEQCFHTGAMLAKMHLAAADFPLEMENPRYDAWWTEACARLLPVLSQ
                     DDAALLCSEIDALKDNLGNHLPSGIIHADLFKDNVLLDGGQVSGFIDFYYACRGNFMY
                     DLAIAVNDWVRTADNKLDEALKKAFIGGYEGVRPLSAEEKAYFPTAQRAGCIRFWVSR
                     LLDFHFPQAGEMTFIKDPNAFRNLLLSLG"
     gene            complement(9773..10501)
                     /gene="ubiG"
                     /locus_tag="WGQ68_001242"
     CDS             complement(9773..10501)
                     /gene="ubiG"
                     /locus_tag="WGQ68_001242"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEZ0715036.1"
                     /translation="MGGKMSDKKYNVDEGEIAKFSRIADKWWDKSGEFKTLHDINPLR
                     LDYIDGHADLRGKRVLDVGCGGGILAESMARRGAAFVKGIDMAEQSLETARLHAALNN
                     VADIEYECIRVEDLAEAEPHSFDVVTCMEMMEHVPDPAAIVRACANLVKPDGMVFFST
                     INKNPKSYLHLIVAAEYLLKFVPKGTHDWKKFIAPAELARMCRQAGLDVADTKGMTYH
                     VLSQTYALCDSTDVNYMFACRPAF"
     gene            10653..11894
                     /locus_tag="WGQ68_001243"
     CDS             10653..11894
                     /locus_tag="WGQ68_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235268.1"
                     /note="tryptophan transporter of high affinity; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HEZ0715037.1"
                     /translation="MPTKTPSLFGGAMIIAGTVIGAGMLANPTATSGVWFTGSLAVLL
                     YTWFSMLSSGLMILEVNTHYPHGASFDTMVKDLLGRSWNIINGIAVAFVLYLLTYAYI
                     FVGGDLTAKGLGSAAGGNVSLTVGQLVFFGILAFCVWASARLVDRFTSVLIGGMVLTF
                     IWATGGLIADAKLPVLFDTQAPTGTNYWIYVATALPVCLASFGFHGNVSSLLKYFKGD
                     APKVAKSIWTGTLIALVIYVLWQTAIQGNLPRNEFAPVIAAEGQVSVLIETLSKFAQT
                     GNMDKILSLFSYMAIATSFLGVTLGLFDYIADIFKWNDSVSGRTKTAALTFLPPLISC
                     LLFPTGFVTAIGYVGLAATVWTGIIPAMLLYRSRKKFGAGKTYKVYGGLWLMVWVFLF
                     GIVNIAAQVLSQMELVPVFKG"
     gene            complement(11964..13615)
                     /locus_tag="WGQ68_001244"
                     /pseudo
     CDS             complement(11964..13615)
                     /locus_tag="WGQ68_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
     gene            13785..14354
                     /locus_tag="WGQ68_001245"
     CDS             13785..14354
                     /locus_tag="WGQ68_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0715038.1"
                     /translation="MNYKIYDGAKIIKKENLSIGDYSQIDDFVFFNCGEKSILGSFVH
                     ISSFTSIIGGGEFYMDHFSGLSAGCRIITGSDDFMGGGLTNPTVPSKYTNVKKSSVRI
                     GKHAILGTNVTVLPGVTIGDGVAVGAGAIVRKNLEPWTIYVGVDCKPLKRRSQDKILE
                     LEKHLVAEFNLDLCPIIPSPTRPNPKETR"
     gene            14351..14914
                     /gene="gmhB"
                     /locus_tag="WGQ68_001246"
     CDS             14351..14914
                     /gene="gmhB"
                     /locus_tag="WGQ68_001246"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HEZ0715039.1"
                     /translation="MKLIILDRDGVINQDRDDFVKSVDEWIPVEGSMDAVAFLTQAGY
                     TVAVATNQSGIGRKYFTVQNLTEMHAKMHRLVRQAGGEINGIWFCPHTGADGCGCRKP
                     KPGMIEDILERFNAQASETWLVGDSLRDLQAIDAVGGKPALVLTGKGKKTLSQHGHEL
                     PEHTQVFDTLLDFSQYIMQENAAPQAD"
     gene            14944..15687
                     /locus_tag="WGQ68_001247"
     CDS             14944..15687
                     /locus_tag="WGQ68_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEZ0715040.1"
                     /translation="MLIIRNLIYWLILCSTLIFLFPFMLLASPFRDGAHKMARVWVKI
                     LNLSLKHIVGLKYRIIGAENIPDRPAVICAKHQSGWETLALQDIFPPQVYVAKRELFK
                     IPFFGWGLKLVKTIGIDRNNRREANEQLIKQGLARKNEGYWITIFPEGTRLAPGKRGK
                     YKLGGARMAKMFEMDIVPVALNSGEFWPKNSFLKYPGEITVVICPTIPHASGSEAELM
                     EKCEHLIETQQPLISGAGPFAAKMPSETA"
     gene            15684..16376
                     /locus_tag="WGQ68_001248"
     CDS             15684..16376
                     /locus_tag="WGQ68_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEZ0715041.1"
                     /translation="MTAFVHTLSDGMELTVEIKRRAKKNLIIRPAGTHTVRISVPPCF
                     SVSALNRWLYENEAVLRQTLAKTPPPQTAENRLPESILFHGRQLALTAHQDTQILLMP
                     SEIRVPEGAPEKQLALLRDFLERQAHSYLIPRLERHARTTQLFPASSSLTSAKTFWGV
                     CHKTTGIRFNWRLVGAPEYVADYVCIHELCHLAHPDHSPAFWELTRRFAPYTPKAKQW
                     LKIHGRELFALG"
     gene            16334..16654
                     /locus_tag="WGQ68_001249"
     CDS             16334..16654
                     /locus_tag="WGQ68_001249"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715042.1"
                     /translation="MAQNPRQGTFRLRLTRPGQASANSDLLPNGHPESSITAGDTYRC
                     LRFQTASVPEQARARPIQTAMTLCRRFGAGWRHTRRLPRFISHRSSETRNPPGPTCGM
                     KRLS"
BASE COUNT         4070 a         5122 c         4290 g         3698 t
ORIGIN      
        1 atttgcggtc aaccgttttc ttgaaaaaat tttataaaaa tatttatcat attgtttata
       61 aattaattta tatttattaa cctgcttttg ccatataaaa ttcttccagc gcatgagaga
      121 tattttcagt ctgccattcg ttttttaagg gtgcaacaat ttcgatttgt cggttttggt
      181 aggcaaattg tattctccat gcatacagaa acatggtttc ggattctgtt ccgccgtata
      241 agctgtcgcc cagaatcgga ctgcccaaac ttttcatcgc cactctcaat tggtgcgttt
      301 tgcccgtatg cggttccagg atgaacagcc gcagtttttc ggcgatactg atgctgtgga
      361 atcgggtaac ggcgatattt tctgtattgc gcgtcaactt ccacattccg cgtctggatt
      421 tttccattcc gcctttaatc caaccctgct ttttggacgg tttgcggtcg gatagtgcca
      481 aataggtttt tttgatgctt ttgccggcaa actgtccgct aagggcggac gcgctttctt
      541 tgttgagggc aaacagtaaa atgccgctgg tctgtttgtc caatcggtgc agcagccaca
      601 cacgctctac gcccaactgt atggcgagtg ttcgggccag tccggtctcg ccgctgtctt
      661 ggtggacgga tatgccgccc ggtttgttga tggcgacgaa gtcttgatgg cggaacaaaa
      721 tttccaacat atccatatat gccttgcaaa aatagaaggg ttcaattttc gtgttgatgt
      781 tcggcaagga ttttttcgta cacagcttgc ggcacgtagc ggtggatcgt ttccgtccag
      841 ccttccggcc cgaccaagcc tttgaccata gtggacgaca cttcggcgat ttcgcgcggc
      901 ggcatgagga atacggtgga tatttcgggg gcgaggtcgc tgttgatatg gcgcatggaa
      961 cgttcgtatt cgtaatccga agcagaacgg atgccgcgca cgatgaatcc tgcatctacc
     1021 tcacgggcgt aatgcaccag aaatcggttt tcaaatacat cggttctgac gttgggaaac
     1081 attttagtaa tatcgcacaa catatcctgc ctttcagcga cggtataggt gctgcgtttg
     1141 tcggggttaa tgccgatggc gacgatgagt tcgtcaaaca tagattgcgc ctgccgtatc
     1201 atccacagat gccccaatgt gggcggatcg aaactgccgg cataaacggc gcggcgcggt
     1261 gtattcggta acatttgatt cctcccggct tcatagtcgg ctgtgtgttg gtgcgtgtgc
     1321 atccgtattg tatgcccaaa gtaaaatgcc gtctgaagca ttttcagacg gcatagtcgg
     1381 acggcgtttt accggcatca atcctcgccg tttaaagaca acaggatgtt cagcaggctg
     1441 ctgaagatgt tgtaaagcga gataaacagt gtcagtgccg cgctgatgtg gctgtcttcg
     1501 ccgccgtcga tgacggtgcg tacctgccac ataatcatta aggaactgaa caagacaaaa
     1561 ccggcggaaa tggtcagggc gagtgcggga atacccaaaa acagattggc aaccacggcg
     1621 accatcagaa tgaccgcacc tacggtcagg aagcgtccga gcgcgttcat atcgagccgg
     1681 gttcggcgcg ccaaggcgga catcgttaaa aagacggcgg cggtcatcgc ggcggcaatg
     1741 ccgacgattt tcgcaccgtc ggcaatatgg agcgcgtatt gcagcacggg gccgatcaat
     1801 acgcccatac cgaatgtgaa taccatcagc agggtaacgc cggtattgct gtaacggttt
     1861 ttctcgatga agtggatcat accgtagaaa aacgccaaca cgacggcaaa ccctatccag
     1921 cgcgaaccga aggcggcgta aaaattgaaa ccggcattgg cggcaagtgc cgcgcctgcg
     1981 gcagccggaa tgaatgaaaa tccgagcagg cggtaggttt tctgcaggac ggtgttttta
     2041 gaaaccgtat gcgcggtgta gtcgtaaacg tcgtgttgca tatcatctgc tcctgaaagc
     2101 gcggttggga ataatggggg attttaacat tgcccaatgt caaaatttgt ccggttgcgt
     2161 gaagataaag ttgtccggcg tattttaaag gccgtctgaa gcagtttcgg acagcctgtg
     2221 ttcaaaacgg aaaaccgtta ttgcggaacg tatccctgaa cggcatccgc gccgtcgccg
     2281 aagaaatact gctccatctg ctgagccagg tattcgcgcg cgcgcggatc ggcgaggctt
     2341 aaacggtttt cgttaatcag catcgtttgg tggcgcgtcc acgccgccca cgcttcttgc
     2401 gatacgtttt caaaaatgcg cttgcccaat tcgttgggaa gcggtggaaa tttcatgcct
     2461 tcggcttctt tgttgagctt gacgcagaat accatgcgtg ccatagcgga ttcctttgct
     2521 gtgttcagaa ataacggggt aattttaacc gattagggat acggacaaaa gccttcttat
     2581 tcccgatgat agggatggtt gtgcaggatg gaggcggcgc ggtagagctg ctcggtcaga
     2641 aggacgcgca ccatcccgtg cggcagggtc aggctggaca ggcgcatcat tatgcgggct
     2701 tgttgtttga ggcggtcggt cattccgtcc gcgccgccga tgacgaagca gacgtgttcg
     2761 ccgttttgcc gccagctttt gaggtgttcc gccagctcga cggaggtcgg cgctttgccg
     2821 cgttcgtcaa gaacgacaag gaacgcgcct tgcggaacgg cttcaaggat gcgtttttct
     2881 tccgccgcca taccttgggc ggcattcacg cccgcgccgc gtttttcggg tttgatttct
     2941 ttgagtgcgt aggcgacgtc gcgtccgaag cgtttggcgt attcggcgac ggcctcatca
     3001 acccagcgcg gcattttggt gccgactgcc aaaacggtaa tgttcaatgc tttctccctt
     3061 acaggaaaat gccgtctgaa cattcagacg gcatcgggaa tcagtctgcc gcgtgccacg
     3121 gcttctgcat tccggcgtgg aaactcggtt tctcgccgcc ccaaagggtg tcgatgtcgt
     3181 agaagtcgcg cacggcaggg agcataacgt ggacaacgag gtctcctgca tcaaccaacg
     3241 tccattcgcc gctgtcgcct tcggtactga ggatttcaaa accggcttct ttcaaatcga
     3301 cggcaacgtt gttggccagt gctttgactt ggcgcgtact gtcgccgctg gcgataatca
     3361 ttctggcaaa cagcgaagtt ttatcttgcg tttcgagaac ggaaatgtct ttggctttga
     3421 tgtcttcgag ggcgttgacg gcgaccccga ccattttttg caggtcttgc agttcttgtt
     3481 cgttcattat gttcctaacg ggatgttttc agacggcatt atagccgttt cttactgatt
     3541 tgactttatt tttcatacaa accgtgttcg cggatgtagc gtgcggcggc aggcgggatg
     3601 ccgtctgaaa cgccttggcc ggcaaggttg cggcggattt ccgttgacga cacattatgc
     3661 atcggggcgg acaagatgcg gacgctgccg tcctgaagcg cattgcccag ccacgcgtgc
     3721 agttcgcgcg gggtttggtg caggctgtcg ccctgccgca tggcgacggc gatattggtt
     3781 tcgcgcacga gcatctgcca ttttttccat gtgtgcagct tcatcaggct gtcgctgccc
     3841 atcagccacc agagttgcgc cgaggggaac tgctggcgga aaatttggac ggtgtcgaaa
     3901 gtgtaagtct cgccccggcg gacgatgtcg cagtcgctga cggcaaaacg cgcatcttct
     3961 gccgtcgcca attcgaccat ggcaaggcgg tcggcggcgg aagcggaggc ggcgtctttg
     4021 tgatacgggc cgcctgtcgg caggaaaaca accgcgtcca agccgatttc gtcggcaaag
     4081 gcgcgggcga tgtggagatg cccgttgtgt atcgggtcga aagtgccgcc gaacagtccg
     4141 attttcttca tgatgtttcc attccttccg ataagtccat gccgtctgaa acactgccgt
     4201 ccacaaccag cgtcagtttg ccggtaacgg gatggatgac cagtccgtga acggcgatat
     4261 ggcgcggcat cagcggatgg ttacggataa ggtccaccgt gtggcgcaca ctgtcttcga
     4321 cgttgtcgaa accggtcagc cagccgtcga ggtcgatacc ggcataacgc agggtttcga
     4381 tacggtcttc gggaatctgg ctttcccgga cgcgcccgag gaattcttcg gcattcagcc
     4441 cctgcatacc gcaatcgtga tgggcgatga ccataatctc tctgaccttc aattcaaaca
     4501 cggcaaccaa aagactccgc atcaccgaac cccacgggtg cgtaaccagc gcgccggcat
     4561 ttttaatcag cttggcatcg ccgtttttca aacccaacgc gtcgggcagc agcccgataa
     4621 tccgcgcatc catacaggac aaaactgcca gcccgcgttc ggggtatttg tcggtaaagt
     4681 atttttcata ttcgcccgac tcgacaaact gccggttatg ggcaaggatg ttatccaact
     4741 cgctcatttt gccgtcctct gaaaaagggt tcgcattata acgtttccgt ccgttttccg
     4801 ccttcgccgc cgtccaacag caggaaaata cccagcgcga acgccgccaa cagcaatgtc
     4861 agcaccatcg cccgcgcgta attatcctca cccgcgcgtc ccaaataggc ataaatcaaa
     4921 gtcgtcagcg tctgccattc cggacgcgac agaaacaatg tcgccgcaaa ttcgcccaca
     4981 cacgtcgccg ccgccaaagt cagaccgcgc cgcaacgccg gtttcaagag ggggaacgtg
     5041 atgcggcatg ccgtctgaaa gccgtttgca cccaaacccg ccgccgccct gccgtaatcc
     5101 ggcggcagtg catcccaggc tgataaaaca tcttttgcca caaacggata cgccagcagc
     5161 gcatacatcg ccagcagcag cggcaacgaa gccgtccact gcggataaag cagcagcacg
     5221 cccgccgaaa cacaaaccgg cgacaccata aacggcaaaa acatcagccc gcgcatccac
     5281 gccgaccgcc gcgccgccgc cgcatacacc acacccaaaa ccgccgctgc atacaccgcc
     5341 gccgccgaga agcgcaaagt attccacacc gcctgccacg tttcactttc cattaacaca
     5401 cgccacgatt cgccggccga ccacgctttc acaacaattg ccaacaaagg aaacaggcag
     5461 cacacagaca acaccgccgc cgcaaacgcc agcagcacat attccccgac cgactgcggc
     5521 ggcgacggca tcacagggga aaccgcctta tccgaaaccg cgcgcctgcc gaaccacgca
     5581 tacagcaacc ctgccgccgc cgttaccccc aacaccagcc acaccagcac cgaggcaacc
     5641 gccatatcga gttcaaacat gaccaactgg taaatttcca cttcgaccgt ggcataacgg
     5701 ctgccgccca gcagcaatgc cagcccgaac ccggaaaaac aatacaggaa gacaaggcac
     5761 acgccgccgg caagccacgg gcgcaaaacg ggcatttcaa tgtcccaaaa ccgccgccac
     5821 gcccccgcgc ccaacgtccg tgccgtctga agccgtgccg caggcacttg cacaaacccc
     5881 tgatacgccg ccctgaccaa cacaggaagg ttgaaaaaca cattgccgta caacaacaga
     5941 tacggcgtat cctgcctgcc gcgccacaac agcccgtccg ccccgaacag ggccagcacg
     6001 cccacgcccg ccaccaacgt gggcatcaca aaaggcagca tcagcaggcg cagcaccaaa
     6061 gcccgccccg gaaacgccag ccgcgccagc acccacgcga caggcacgcc caaaggcagc
     6121 accagcacac aggttgccgc tgcctgaaat accgtccacg ccaaacgttt gagcatatag
     6181 gcatccgaca gcaccgcgcg ccacgccaaa ccgtcatacg ccgccaccgc ccacaaaggc
     6241 gcaacgacca ttaccgccaa aaaagccgaa ggcagcaggg caaaagcacc ccataccgcc
     6301 caacgccgtc catccatcgc cttccccact tgaaacactg atgttgcgat tgtacccaaa
     6361 agcccccaca taccgtatat ttcaatccga ctacataccg tatccgcctt cctcccgccg
     6421 tctgaaataa atcgttcaaa taaacaggaa tataacttca gacaaacaac ttaccgcccg
     6481 atttgtgcta tcgttttcgc acaacttaaa aaaacctgac aattttgtac ttttattaca
     6541 gagaaaggct ttacaaatgg acggctggac acagacgctg tccgcgcaaa ccctgttggg
     6601 catttcggcg gcggcaatca tcctcattct gattttaatc gtcaaattcc gcatccacgc
     6661 gctgctgaca ctggtcatcg tcagcctgct gacggctttg gcaaccggtt tgcccacagg
     6721 cagcattgtc aacgacgtac tggtcaaaaa cttcggcggc acgctcggcg gcgtggcgct
     6781 tctggtcggc ctgggcgcga tgctcggacg tttggtcgaa acatccggcg gcgcacagtc
     6841 gctggcggac gcgctgatcc ggatgttcgg cgaaaaacgc gcaccgttcg cgctgggcgt
     6901 tgcctcgctg attttcggct tcccgatttt cttcgatgcc ggactaatcg tcatgctgcc
     6961 catcgtgttc gccaccgcac ggcgcatgaa acaggacgta ctgcccttcg cgcttgcctc
     7021 catcggcgca ttttccgtca tgcacgtctt cctgccgccc catccgggcc cgattgccgc
     7081 ttccgaattt tacggcgcga acatcggcca agttttgatt ttgggtctgc cgaccgcctt
     7141 catcacatgg tatttcagcg gctatatgct cggcaaagtg ttggggcgca ccatccatgt
     7201 tcccgttccc gaactgctca gcggcggcac gcaagacaac gacctgccga aagaacctgc
     7261 caaagcagga acggtcgtcg ccatcatgct gattcccatg ctgctgattt tcctgaatac
     7321 cggcgtatcg gccctcatca gcgaaaaact cgtaagtgcg gacgaaacct gggttcagac
     7381 ggcaaaaata atcggttcga caccgatcgc ccttctgatt tccgtattgg tcgcactgtt
     7441 tgtcttggga cgcaaacgcg gcgaaagcgg cagcgcgttg gaaaaaaccg tggacggcgc
     7501 actcgccccc gtctgttccg tgattctgat taccggcgcg ggcggtatgt tcggcggcgt
     7561 tttgcgcgct tccggcatcg gcaaggcact cgccgacagc atggcggatt tgggcattcc
     7621 cgtccttttg ggctgtttcc ttgtcgcctt ggcactgcgt atcgcgcaag gttcggcaac
     7681 cgtcgccctg accaccgccg ccgcgctgat ggctcctgcc gttgccgccg ccggctttac
     7741 cgactggcag ctcgcctgta tcgtattggc aacggcggca ggttcggtcg gttgcagcca
     7801 cttcaacgac tccggcttct ggctggtcgg ccgcctcttg gacatggacg taccgaccac
     7861 gctgaaaacc tggacggtca accaaaccct catcgcactc atcggctttg ccttgtccgc
     7921 actgctgttc gccatcgtct gacagacgga aaggatagta aatgactacg cattttgtcg
     7981 ttatgggcgt atgcggctgc ggcaagacca ccgccgcgct gtccctgcag aaacacctcg
     8041 gtcaatgtcc ctatgccgaa ggcgacgagt tccacaccca agccaaccgc gacaagatgg
     8101 gcgcgggtat tccgctgacc gatgaagacc gctatccgtg gttgggcaat ctgcgcgact
     8161 ggatgacgca acaggcgcaa aacggtgcga accacaccat cgtaacctgt tccgccctca
     8221 aacgcggcta ccgcgacatt ctgcgcggag ccgaaggcaa agctgccttc atccacctca
     8281 gtccgccgca agacatcaac ctcgagcgca tgatgtcgcg caaaggacat tacatgaaag
     8341 cagggatgct cgattcgcaa ctggaaatcc tcgaggaact gggcgaaggc gaatacgggg
     8401 tcaaaatcgc caaccccggc acgcccgaag cggtcgaagc cgatattctg aactgggttg
     8461 cctcggaaaa cctgctttga agcaatatgc cgtctgaagc ccgacacagg atgggtttca
     8521 gacggcataa acatcgggaa cagaatggat tacattgatt tatagtggat taacaaaaac
     8581 cagtacagcg ttgcctcgcc ttagctcaaa gagaacgatt ctctaaggtg ctgaagcacc
     8641 gagtgaatcg gttccgtact atctgtactg tcttcggctt ggtcgccttg tcctgatttt
     8701 tgttaatcca ctataaaatg gaaaataccc ggctatcgtc tcattttcgt tttaatcagc
     8761 cataaaaatg ccgtctgaaa ccctttcaga cggcatttct gtcaaacgcc ggacgcactc
     8821 aacccaaact caacagcagg ttgcggaacg cgttcgggtc tttgataaac gtcatctcgc
     8881 ccgcctgcgg aaaatgaaaa tccaacaggc gcgacaccca aaaacggatg cagccggcgc
     8941 gttgggcggt cgggaaatac gccttttctt cggcactcaa ggggcgcacg ccctcataac
     9001 cgccgataaa cgcctttttc aacgcctcat ccaacttatt gtccgccgtc cttacccaat
     9061 cgttgaccgc aatcgccaag tcatacataa aattgccccg gcaggcataa tagaaatcga
     9121 tgaagcccga tacctgaccg ccgtcaagca acacattgtc tttaaacagg tcggcgtgga
     9181 tgatgcccga aggcagatga ttgccgagat tgtccttcaa cgcatcgatt tcggaacaca
     9241 gcagtgcggc atcgtcttgc gacaggacgg gcagcagtcg ggcgcacgcc tccgtccacc
     9301 acgcatcgta acgcgggttt tccatttcca aagggaaatc ggcggcggca aggtgcattt
     9361 tggccagcat cgcaccggta tggaaacact gctccgccgt cggcagcgcg gtatccgaac
     9421 ctttcaggca ggcaaccaaa caagccggct tgcccgccaa aacggaatca agccggccgt
     9481 ctttgcgcgc aaccggcgcg gcaaccgcca cgcccttcat actcaaatgc cggttaagct
     9541 ccagaaaaaa cggcagctct tcctgtttca acacttcaaa cacggtcagc acataacgtc
     9601 ccgaagtcgt cgtcagaaaa taattgctgt tggtaatccc ctgcgcgatg ccctgcaggg
     9661 aaacaaattc ccccaaatcg taaccgctca ggaagccgcg catttcatca tcggaaacac
     9721 tggtatagac agacatatca actcacttgc tcaaaaacgg gcaaacccgc cgtcagaacg
     9781 ccggacggca ggcaaacata taattcacat cggtcgaatc gcacagggca taagtttgcg
     9841 acaacacatg gtaagtcata cccttcgtat ccgccacatc caagcctgcc tgacggcaca
     9901 tccgcgccag ctcggcaggc gcgatgaatt ttttccagtc gtgcgtgcct ttggggacaa
     9961 acttcaacag atattccgcc gccacaatca gatgcaggta cgatttcggg tttttattga
    10021 tggtggaaaa aaacaccatg ccgtccggtt tgaccagatt ggcacaagca cgcacgatgg
    10081 cggcgggatc ggggacgtgt tccatcattt ccatgcacgt taccacatcg aacgagtgcg
    10141 gttccgcctc ggcaaggtct tccacgcgga tacattcgta ttcgatatcg gcgacattgt
    10201 tcaaagccgc gtgcaggcgg gcggtttcca acgactgctc cgccatgtcg atgcccttta
    10261 caaacgccgc gccgcgccgc gccatacttt ccgccaagat gccgccgccg cagcccacgt
    10321 ccaaaacccg tttgccgcgc aaatccgcgt gtccgtcgat ataatccagc cgcagcggat
    10381 tgatgtcgtg caaggttttg aactcgcccg acttgtccca ccatttgtcg gcaatccggc
    10441 tgaatttggc gatttccccc tcatcgacat tatatttttt gtcggacatt ttccctccca
    10501 tctgacgaac cgcccactcc aaaacccaag attatactat ggaacgcaat gccgtctgaa
    10561 agccttttcg ggcggcgcgg cgcaaatacc ttacaaatcc ttacacttta cggcataatg
    10621 gcggctcgct ttttctggca gaaagacaaa atatgcccac caaaacccct tcactgttcg
    10681 gcggcgcgat gattatcgcc ggcacggtca tcggcgcagg tatgctcgcc aacccgaccg
    10741 ccacatccgg cgtatggttt accggctcgc tggccgtgtt gctgtacacc tggttttcca
    10801 tgctctccag cggcctgatg attttggaag tcaacaccca ctacccccac ggcgcgagct
    10861 tcgacaccat ggttaaagac ctgctcggac ggagctggaa catcatcaac ggcatcgccg
    10921 tcgccttcgt tttatacctg cttacttacg cttatatctt cgtcggcggc gacctgaccg
    10981 ccaaaggctt aggcagcgcg gcaggcggca atgtttcact caccgtcgga caactcgtct
    11041 tcttcggcat tctcgccttt tgcgtatggg catccgcacg cttggtcgac cgattcacca
    11101 gcgtcctcat cggcggcatg gtattaacct ttatttgggc aaccggcggc ctgattgccg
    11161 atgccaaact gcccgtcctc ttcgacaccc aagcccctac cggcaccaac tactggattt
    11221 atgtcgccac cgccctgccc gtctgccttg cgtcattcgg tttccacggc aacgtctcca
    11281 gcctgctcaa atactttaaa ggcgacgcgc ccaaagtggc taaatccatc tggacgggca
    11341 cactgattgc gctggtaatt tacgtcctct ggcaaaccgc catccaaggc aacctgccgc
    11401 gcaacgagtt cgcccccgtg attgccgccg aagggcaagt ctccgtcctg attgaaaccc
    11461 tgtccaaatt cgcccaaacc ggcaatatgg acaaaatatt gtccctgttt tcctatatgg
    11521 cgatcgccac ctcgttttta ggcgtaacgc tcggactctt cgactacatc gccgacatct
    11581 tcaaatggaa cgacagcgtg tccggccgca ccaaaaccgc cgcgctgacc ttcctgccgc
    11641 ctctaatttc ctgcctgctc ttccccaccg gctttgttac cgccatcggc tacgtcggcc
    11701 tggcggcaac cgtctggaca ggcatcatcc ccgccatgct gctctaccgt tcgcgcaaaa
    11761 aattcggcgc aggcaaaacc tataaagttt acggcggctt gtggctgatg gtttgggtct
    11821 tccttttcgg catcgtcaac atcgccgcac aggtattgag ccaaatggaa ctcgtccccg
    11881 tatttaaagg ataaaggcaa aatgccgtct gaacgagttt tcagacggca ttttcgcaac
    11941 cgttgcagcc atgcggcaaa aagctaacgg gcaattttca aaacgtcgta cagatagttg
    12001 cggtaatttc cgaccaactc ccagttcggg cagtcttcca accctccgcc gcacaaaccg
    12061 cgcatcgcca tattcgcacg catgaagccg tctttggctt ccagaccagc cgcctccatc
    12121 agcacgccgc ctgcaaaggc aaggtcgagg aaattctgcc gctgtacgaa tccgccggca
    12181 atattgctcc tgacggcaaa ctgcgttaca taatccggct gcgcgtaatc ccatttcttc
    12241 ctgacggaca cgccctcaaa cggtacctga tgatcgccga aataaccgaa aacaaagggt
    12301 ttgccgcgtt cgtgcagata acggtcgaaa ctttccaccg ctttgtccaa atcggcaatg
    12361 cgcccgatgt agtcgttaag cgcggatacg gttttcgcat tcaaatcggg cgcatccaaa
    12421 tcaaacacat tgtcggtatc cgtccgatac ggcccgtgct ccttcatggt cagcacatat
    12481 acgaacatcg gctgccgcac gttttccaaa tcgggatggc gtttttccaa aatcatccgc
    12541 gcatactgca tcatttcctc actggaaata tgccacaggt ttttgcccat cggcgcggga
    12601 tagcccaaat cttgcggctg gaacatcaga ttaaagccga aatggtcata tgctgccttg
    12661 gcattgtagt tgcccttggt aaatggcgag agtgccacgc aaaaataacc gtgttcgcgc
    12721 aggttgcgga caaaaccggt ctgcaaatgc ggtacgaccg agtaaaacac gccgcttgcc
    12781 aacgcgccga aatccgtgga cggaacaccc gccaaaaacg caaattcgga cttccacgtt
    12841 gcgccgccga aagtatgcac gcgcaaaggt gaggaaaata cggtatcttc ctgccgtccg
    12901 aacattttca aatcgggaat cttggcggcg gcaaagtcga aacagtgcgg atcgagcgtc
    12961 gattccatca gggtaacgac aatatcgggt ttttcatccg acatgccgca cggccgggtt
    13021 tcagacggca tctgcctggc aaacgcttcc ccgtcgccct cgaacacggg tacttggaaa
    13081 aacaccgccc gacaggacat cggcaggttc aaaacacgtc gcgcccgtcg tccggcagcg
    13141 aatccagcca cgtctttacc gcgcccggat gtttggaaaa atgccgcatc gacacgaacg
    13201 ccgccgcaaa caaaaccgcg cctgcccacc gccacggcac atccaaagaa tccgcaccgc
    13261 tccagccgaa aaccgcatat gccgccaaag ccagcagccc cgccataccg ataaccgctt
    13321 ccttataatg gaacagcgtt tcccaattcc gccagtcggc aatcagcaaa aagtcggaaa
    13381 tcaacagcgg ctgtttgtaa taatggattt tcagcctgtg gaacagtatc agcaccacaa
    13441 accagaccga agcgaaattc aaggcacgtt gccactgagc cgtcaacatc agcataccgc
    13501 ccgccaaaaa gacaaacagc gcggatgcga aaaaatacgt ccagcggtag tgcgacctga
    13561 taatcagcac caacgccgcc gttacaaaca aaaacaggaa aacataggca accatcccgc
    13621 cccactcctc aaaacggcat acctgagccg ttgcgaatta ttatcgaaag cgtgcattat
    13681 aatgcagcac aggcgcatca ctgccgatat ttcagtataa ttattcgaga tggaatcact
    13741 gaaaatggtg aagttacaat gagccctttt agaggaacta aaaagtgaat tataaaatat
    13801 acgatggtgc aaagattatt aaaaaagaaa atttatcaat tggggattat tctcaaattg
    13861 atgattttgt attttttaat tgtggagaga aaagtatttt aggaagtttt gttcatatat
    13921 catcatttac cagtattatt ggtggtggag aattttatat ggatcatttt tcaggattaa
    13981 gcgcagggtg tagaattatt accggtagcg atgattttat gggggggggg ttaaccaatc
    14041 caacagtccc atctaaatat actaatgtta aaaaatctag tgttagaatt ggaaaacatg
    14101 caattttagg aacaaatgta acagtattac caggcgttac tattggtgat ggggttgctg
    14161 ttggagcagg ggctatagtg agaaaaaatc ttgagccgtg gactatttat gtcggggtag
    14221 attgtaaacc actgaagcgc agatcacaag ataaaatatt ggagttagag aaacatcttg
    14281 tagcagaatt taatctagat ttatgtccta taatcccatc cccgacacgc cccaacccaa
    14341 aggaaacgcg atgaaactca tcattctcga ccgcgacggc gtcatcaatc aggaccgcga
    14401 tgacttcgtc aaatccgttg acgagtggat acctgtcgaa ggcagcatgg atgcggtggc
    14461 atttctgacg caggcaggct acaccgtcgc cgttgccacc aaccaatccg gcatcgggcg
    14521 caaatatttt accgttcaaa acctcaccga aatgcatgcc aaaatgcacc gcctcgtccg
    14581 tcaggcaggc ggcgaaatca acggcatctg gttctgccct cataccggcg ccgacggctg
    14641 cggctgccgc aagcccaaac cgggtatgat tgaagacatc ctcgaacgct tcaacgccca
    14701 agcttcggaa acttggctgg tcggcgacag cctgcgcgat ttgcaggcaa tcgatgccgt
    14761 cggcggcaaa cccgcgctgg ttctgaccgg aaaaggcaaa aaaacgctct cccaacacgg
    14821 acacgaattg cccgaacaca cacaggtttt cgataccttg ctcgatttct cacaatacat
    14881 catgcaggaa aacgccgcac cgcaagccga ctgaacatac cgcatttccg acaaggcaaa
    14941 accatgctca tcatccgcaa cctgatttac tggctgatac tctgttccac cctgattttc
    15001 ctctttccct ttatgctgct cgcctcgcct ttccgagacg gggcgcacaa gatggcgcgg
    15061 gtctgggtca aaatcctcaa cctctcgctc aaacacatcg tcgggctcaa ataccgcatc
    15121 atcggcgcgg aaaacatccc cgaccgcccc gccgtcatct gcgccaaaca ccaaagcggc
    15181 tgggaaacgc tcgcccttca ggacattttt ccgccgcagg tttacgttgc caaacgcgag
    15241 ttgttcaaaa tccccttttt cggctggggc ttgaaactgg tcaaaaccat aggcatagac
    15301 cgcaacaacc gccgcgaagc caacgagcag ctcataaaac aggggttggc gcgcaaaaac
    15361 gaaggctatt ggattaccat tttccccgaa ggcacgcgcc ttgcacccgg aaagcgcggc
    15421 aaatacaaac tcggcggcgc gcgcatggcg aaaatgtttg agatggacat cgtccccgtc
    15481 gccctcaaca gcggcgaatt ttggccgaaa aactcctttc tgaaatatcc gggggaaatc
    15541 accgtcgtca tctgtccgac catcccgcac gcaagcggca gcgaagccga attgatggaa
    15601 aaatgcgaac acctcatcga aacgcagcag ccgctcattt ccggcgcagg cccgtttgcc
    15661 gccaaaatgc cgtctgaaac cgcatgaccg cctttgtcca caccctttca gacggcatgg
    15721 aactgaccgt cgaaatcaag cgccgtgcca agaaaaacct gattatccgc cccgccggca
    15781 cacataccgt ccgcatcagc gtcccaccct gcttctccgt ctccgctcta aaccgctggc
    15841 tgtatgaaaa cgaagccgtc ctgcggcaaa cactggcgaa aacaccgccg ccgcaaactg
    15901 ccgaaaaccg gctgcccgaa tccatcctct tccacggcag acagcttgcc cttaccgccc
    15961 atcaagacac gcaaatcctg ctgatgccgt ctgaaatccg tgttcccgaa ggcgcacccg
    16021 aaaaacagct tgcgctgctg cgggactttt tggaacggca ggcgcacagt tacctgattc
    16081 cccgcctcga acgccacgcc cgcaccacac aactgttccc cgcctcctcc tcgctaacct
    16141 ctgccaaaac cttctggggc gtgtgccaca aaaccacagg catacgcttc aactggcggc
    16201 tggtcggcgc accggaatac gttgccgact atgtctgcat acacgaactc tgccacctcg
    16261 cccatccaga ccacagcccc gccttttggg aactgacccg ccgcttcgcc ccctacacgc
    16321 ccaaagcgaa acagtggctc aaaatccacg gcagggaact tttcgcctta ggctgacgcg
    16381 gcccgggcag gcatccgcca attcagacct cctcccaaac ggacatccgg aaagcagcat
    16441 cacagccggc gacacatacc gctgcctccg ctttcagacg gcatccgtgc cggaacaggc
    16501 acgcgcccgc ccgattcaaa ccgcgatgac gctttgccgc cggttcgggg caggatggcg
    16561 gcacacgcgc cgtctgccgc gtttcatttc acaccgctct tccgaaaccc gaaacccgcc
    16621 cggtccgacg tgcggtatga aacgcttaag ctgacgcgaa gtcttttact gatttgcccg
    16681 cgaaaatgcc gtctgaaagg ttttcggacg gcattttttt gcgtttccca ggatggcggc
    16741 ggattcgtaa aaggcggtca gggtggattg taggatgggt tgagacctgc cgaatccgcc
    16801 gcatctgcca aatctaccgc cgtcattcct acgaaagtgg gaatctagaa cgcggggtta
    16861 agaaaacctg catcccgtca ttcccacgaa agtggggatc cagttttttg agtttcagtc
    16921 atttccgata aattgcctta gcattgaatg tctagattcc cgcctgcgcg ggaatgacga
    16981 atccatccat acggaaacct gcaccgcgtc atgcctacga acctgcattc cgtcattccc
    17041 acgaaagtgg gaatccagtt ttttgagttt cagtcatttc cgataaattg ccttagcatt
    17101 gaatgtctag attcccgcct gcgcgggaat gacgaatcca tccgtacgga aacctgcacc
    17161 gcgtcattcc tacgaaccta
//
LOCUS       DAWFHY010000044        16808 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.044, whole genome shotgun sequence.
ACCESSION   DAWFHY010000044 DAWFHY010000000
VERSION     DAWFHY010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16808
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.044"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            157..330
                     /locus_tag="WGQ68_001250"
     CDS             157..330
                     /locus_tag="WGQ68_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0715043.1"
                     /translation="MKSERRHWEWCVDYDWEGVVRDYRFTHKVEKDERSVIRDAVGVI
                     RKEAGKSLSQPEK"
     gene            394..1677
                     /gene="hemL"
                     /locus_tag="WGQ68_001251"
     CDS             394..1677
                     /gene="hemL"
                     /locus_tag="WGQ68_001251"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEZ0715044.1"
                     /translation="MNRNEILFDRAKAIIPGGVNSPVRAFGSVGGVPRFIKKAEGAYV
                     WDENGTRYTDYVGSWGPAIVGHAHPEVIEAMREAALGGLSFGAPTEGEIVIAEEIAKI
                     MPSVERLRLVSSGTEATMTAIRLARGFTGRDKIIKFEGCYHGHSDSLLVKAGSGLLTF
                     GNPSSAGVPADFTKHTLVLEYNNIAQLEEAFAQSGNDIACVILEPFVGNMNLVRPTEA
                     FVKALRELTEKHGAVLIYDEVMTGFRVALGGAQSLHGIMPDLTTMGKVIGGGMPLAAF
                     GGRKDIMECISPLGGVYQAGTLSGNPIAVAAGLKTLEIIQREGFYENLTARTEQLVQG
                     FQTAADAAGIEFTADSVGGMFGLYFAAHAPRNYADMARSNIEGFKQFFHGMLDRNVAF
                     GPSAYEAGFVSAAHTPVLIDETVAVAVEVFKAMAA"
     gene            complement(1893..2165)
                     /locus_tag="WGQ68_001252"
     CDS             complement(1893..2165)
                     /locus_tag="WGQ68_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taxon MazF"
                     /protein_id="HEZ0715045.1"
                     /translation="MPSEAQILHFRRHFYSRLKSKIYTPVIPAQAGIRKVGLVGHEVV
                     LKMKRYRKSDELKEKYSDIDETFEFIKGFIYVIALAFTAYVAIRFF"
     gene            complement(2176..2739)
                     /gene="orn"
                     /locus_tag="WGQ68_001253"
     CDS             complement(2176..2739)
                     /gene="orn"
                     /locus_tag="WGQ68_001253"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HEZ0715046.1"
                     /translation="MQDKNNLCWLDMEMTGLNPETDRIIEVAMIITDSDLNVLAQSEV
                     YAVHQSDELLDNMDEWNTATHGRTGLTQRVRESSHTEAEVEQKLLDFMSEWVPGRATP
                     MCGNSIHQDRRFMVKYMPKLENYFHYRNLDVSTLKELAKRWNPPVAKSVVKRGSHKAL
                     DDILESIEEMRHYREHFLISAPRAEAQ"
     gene            complement(2757..3644)
                     /gene="prmA"
                     /locus_tag="WGQ68_001254"
     CDS             complement(2757..3644)
                     /gene="prmA"
                     /locus_tag="WGQ68_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEZ0715047.1"
                     /translation="MPYQQITVNVNDAVAERLADALMEHGALSAAIEDAYAGTQNEQA
                     IFGEPGMPAEQIWQQSKVIALFGEHDEAAAIIQTAAQECGLKDLAYTGEILEDQDWVR
                     LTQAQFDPIRISERLWITPSWHEAPEGTAVNLRLDPGLAFGTGSHPTTRLCLKWLDTQ
                     LKNGESVLDYGCGSGILTIAALKLGAGSAVGVDIDEQAVRAGKDNAEQNNVDAQFFLP
                     DGLPQGQFDVVVANILANPLRMLGEMLAARTKQGGRIVLSGLLDEQAEELGGIYSQWF
                     DLDPAETEESWARLSGTKR"
     gene            complement(3762..5123)
                     /gene="accC"
                     /locus_tag="WGQ68_001255"
     CDS             complement(3762..5123)
                     /gene="accC"
                     /locus_tag="WGQ68_001255"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEZ0715048.1"
                     /translation="MLKKVLIANRGEIALRVLRACREMGIATVAVHSEADKDSLHVKL
                     ADESVCIGPAASAQSYLNVPAIIAAAEVSCADAVHPGYGFLAENADFAEQVEQSGFTF
                     IGPKPDTIRLMGDKVSAKHAMIAAGVPCVPGSDGALPDDGEEILKIADKVGYPVIIKA
                     SGGGGGRGMRVVEKKEDLLQSVEMTKAEAGAAFGNPMVYMERYLQRPRHVEIQVIADE
                     HGNAIYLAERDCSLQRRHQKVIEEAPAPFITEKERAKIGNACADACKRIGYRGAGTFE
                     FLYEDGEFFFIEMNTRVQVEHPVTELITGVDIVQEQLRIAAGLPLQYKQKDIQVEGHA
                     FECRINAEDPYNFIPSPGPIESCHLPGGFGIRVDSHIYQGYRIPPYYDSLIGKICVHG
                     KTREQAMAKMRVALAELAVTGIKTNTPLHRDLFADAGFQKGGVSIHYLEHWLEDRKAK
                     QDK"
     gene            complement(5237..5698)
                     /locus_tag="WGQ68_001256"
     CDS             complement(5237..5698)
                     /locus_tag="WGQ68_001256"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEZ0715049.1"
                     /translation="MDLRKLKKLIDLVEESGIAEIEVTEGEEKVRITRTIAAAAAPVY
                     AAPVPAAAPAVTPAAAPVAASAPAAAPAARDLSDAQKSPMVGTFYRAPGPNAAPFVEV
                     GQQVKAGDTLCIIEAMKLMNEIEAEKSGTVKEILVENGTPVEFGEPLFIIG"
     gene            complement(5680..5937)
                     /locus_tag="WGQ68_001257"
     CDS             complement(5680..5937)
                     /locus_tag="WGQ68_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase"
                     /protein_id="HEZ0715050.1"
                     /translation="MRKIKLPAHFPQKPPFFDILLDIYRQLRENKHILTVVFHHRKTV
                     SEHHSARFAPLPQAGCFLKNQRNNPPEHRQPLKEQKWICAN"
     gene            6054..7094
                     /gene="queA"
                     /locus_tag="WGQ68_001258"
     CDS             6054..7094
                     /gene="queA"
                     /locus_tag="WGQ68_001258"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEZ0715051.1"
                     /translation="MDISDFDFTLPEHLIAQHPPEVRGSSHLLVALPDMPLQDRVFGD
                     LPDYVEAGDVLVFNNTKVMKARLFGQKDSGGRIEALIERVLDSHTALAHIRSSKSPKP
                     GMGLVFEGGIRAVMVGREGELFCLRFEGGETVYELLEQNGHLPLPPYIERAADADDDS
                     RYQTVYAKYQGAVAAPTAGLHFTEELLRRLKDKGAVTAEVTLHVGAGTFQPVRVDKIE
                     EHKMHSEWFEVPSETAAAVEAAKARGNKVWAVGTTSMRALESAARATGRLKAGQGDTD
                     IFITPGYRFNVADRLVTNFHLPKSTLLMLVSAFSGMGHIRAAYRYAVEREYRFFSYGD
                     AMVLGWNEGVVR"
     gene            complement(7464..8042)
                     /locus_tag="WGQ68_001259"
     CDS             complement(7464..8042)
                     /locus_tag="WGQ68_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005694496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0715052.1"
                     /translation="MNILLLDGGKAFGHSHGGLNRTLHKKAKEVLTALGHNVQETVID
                     AGYDVEAEIEKFVWMDAVIWQMPGWWMHEPWTVKKYIDEVLTAGHGKLYQSDGRHRVN
                     PTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDFFEGKGVDVLYMHFHKANEFLGMT
                     RLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            8281..9180
                     /gene="crgA"
                     /locus_tag="WGQ68_001260"
     CDS             8281..9180
                     /gene="crgA"
                     /locus_tag="WGQ68_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151940473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator CrgA"
                     /protein_id="HEZ0715053.1"
                     /translation="MKTNSEELTVFVQVVESGSFSRAAEQLAMANSAVSRIVKRLEEK
                     LGVNLLNRTTRQLSLTEEGAQYFRRAQRILQEMAAAETEMLAVHEIPQGVLRVDSAIP
                     MVLHLLAPLAAKFNERYPHIRLSLVSSEGYINLIERKVDIALRAGELDDSGLRARHLF
                     DSRFRVIASPEYLAKHGTPQSTEELAGHQCLGFTEPGSLNTWAVLDAQGNPYKISPHF
                     TASSGEILRSLCLSGCGIACLSDFLVDNDIAEGKLIPLLAEQTSDKTHPFNAVYYSDK
                     AVNLRLRVFLDFLVEELGNNLCG"
     gene            complement(9409..12624)
                     /gene="carB"
                     /locus_tag="WGQ68_001261"
     CDS             complement(9409..12624)
                     /gene="carB"
                     /locus_tag="WGQ68_001261"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEZ0715054.1"
                     /translation="MPKRTDLKSILIIGAGPIVIGQACEFDYSGAQACKALREEGYKV
                     ILVNSNPATIMTDPEMADVTYIEPIMWQTVEKIIAKERPDAILPTMGGQTALNCALDL
                     ARNGVLAKYNVELIGATEDAIDKAEDRGRFKEAMEKIGLSCPKSFVCHTMNEALAAQE
                     QVGFPTLIRPSFTMGGSGGGIAYNKDEFLAICERGFDASPTHELLIEQSVLGWKEYEM
                     EVVRDKNDNCIIICSIENFDPMGVHTGDSITVAPAQTLTDKEYQIMRNASLAVLREIG
                     VDTGGSNVQFAVNPENGEMIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGFTLDE
                     LRNDITGGKTPASFEPSIDYVVTKIPRFAFEKFPAADDRLTTQMKSVGEVMAMGRTIQ
                     ESFQKALRGLETGLCGFNPRSSDKAEIRRELANPGPERMLFVADAFRAGFTPEEIHEI
                     CAIDPWFLAQIEDLVKEEKSVSDGILSDLDFAALRRLKRKGFSDKRIAQLLGVKEKEV
                     REHRYALKLHPVYKRVDTCAAEFATETAYLYSTYEEECESRPSDRKKVMILGGGPNRI
                     GQGIEFDYCCVHAALALRESGFETIMVNCNPETVSTDFDTSDRLYFEPLTLEDVLEIV
                     RTENPWGVIVHYGGQTPLKLANALVENGVNIIGTSADSIDAAEDRERFQKVLNDLGLR
                     QPPNRIAHNEEEALVKAEEIGYPLVVRPSYVLGGRAMQIVHSAEALQKYMREAVQVSE
                     DSPVLLDFFLNNAIEVDVDCVSDGKDVVIGGIMQHVEQAGIHSGDSGCSLPPYSLSEE
                     IQDEIRRQTKAMAYALGVVGLMNVQFAVQDGVVFVLEVNPRASRTVPFVSKATGVPLA
                     KVGARAMAGISLKEQGVEKEVVPDFYAVKEAVFPFIKFPGVDTILGPEMRSTGEVMGV
                     GASFGEAYYKAQLGAGERLNPTGKIFLSVREEDKERVIKTAKNFQALGYGICATRGTA
                     QYLTEHGLIVQTINKVPEGRPHIGDALKNGEIALVVNTVSSDPQSVSDSHIIRQSALQ
                     QRVPQYTTTAGGEAMSEGAKSRDHLGVYSVQELHGRLKNRN"
     gene            complement(12664..13275)
                     /locus_tag="WGQ68_001262"
     CDS             complement(12664..13275)
                     /locus_tag="WGQ68_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715055.1"
                     /translation="MINIIATWAMALTYIFLLLFIIVFIPIQLYLENIKKKKFKSRII
                     DILKNNHNNLDLNDIKQMTEAVNLNNFAARKIIKQLYYTDELNLNLVRQLQKEIQQEE
                     PFDGCSDELKPTLIGINELLETHGSESQKHLLTPIISELKELNQIKHDHKKMKTQSYI
                     AYIIAIISFFIGSISFYYTITAPSGKEIANTVVEQLKANQNQQ"
     gene            complement(14123..14512)
                     /locus_tag="WGQ68_001263"
     CDS             complement(14123..14512)
                     /locus_tag="WGQ68_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HEZ0715056.1"
                     /translation="MNPHEKLLTTDNPILRQRAKAMRQEMSGAEAKLWQHLRAGRLNG
                     YKFRRQQPMGNYIVDFMCVTPKLIVEADGGQHTEQAAYDHARTAYLNSLGFTVLRFWN
                     HEILQQTNDVLTEILLVLQELEKQAAR"
     gene            complement(15084..15209)
                     /locus_tag="WGQ68_001264"
                     /pseudo
     CDS             complement(15084..15209)
                     /locus_tag="WGQ68_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687282.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            complement(15255..16388)
                     /gene="carA"
                     /locus_tag="WGQ68_001265"
     CDS             complement(15255..16388)
                     /gene="carA"
                     /locus_tag="WGQ68_001265"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEZ0715057.1"
                     /translation="MSTPALLVLADGSVFHGTSIGYEGSTSGEVVFNTSMTGYQEILT
                     DPSYCKQIVTLTYPHIGNTGTNAEDEESRSVYAAGLIIRDLPLLHSNFRASESLHDYL
                     VRNKTVAIADIDTRRLTTLLREKGAQGGAILTGADATVEKAQELIAAFGSMVGKDLAK
                     EVSCTETYEWTEGEWALGKGFVTPDEQPYHVVAYDFGVKTNILRMLASRGCRLTVVPA
                     QTSAQDVLALNPDGVFLSNGPGDPEPCTYAIEAVQKLMESGKPIFGICLGHQLISLAI
                     GAKTLKMRFSHHGANHPVQDLDSGKVVITSQNHGFAVDADTLPANARITHKSLFDNTL
                     QGIELTDKPVFCFQGHPEASPGPQDVGYLFDKFIGNMKAAKRA"
BASE COUNT         3621 a         4271 c         4654 g         4262 t
ORIGIN      
        1 cagacggaag agggaatcgc acaaacggtt gttaaaggca aaacgactcg ggcggaggta
       61 gaggcacgtt tcgggaagcg caaccctttc ggttgttatg cctatcatga agtcagcctg
      121 ccgatttata attttttgcc gaccaatttc atttatatga aatcagaacg ccggcattgg
      181 gagtggtgtg tggattacga ctgggaagga gttgtccggg actaccgctt tacacataaa
      241 gtggaaaaag acgagcgttc cgtcatccgg gatgcggttg gcgtcatccg caaagaagca
      301 ggcaaatcct tgtcgcaacc tgaaaaatga taaaatggga ctttctgctt ctaagcccga
      361 aacctgccgt tcagacggca tttgaggata aatatgaacc gtaacgaaat tttattcgac
      421 cgcgccaaag ccatcatccc cggcggcgtg aattcccccg tccgcgcatt cggcagcgtc
      481 ggcggcgtgc cgcgcttcat caaaaaagcc gaaggcgcgt atgtttggga cgaaaacggc
      541 acgcgctaca ccgactatgt cggttcgtgg gggcccgcga ttgtcggaca cgcgcacccc
      601 gaagtcatcg aagccatgcg cgaagctgcc ttgggcggtt tgtcgttcgg cgcgcctacc
      661 gaaggcgaaa tcgtcattgc cgaagaaatc gccaaaatca tgccgtccgt cgaacggctg
      721 cgccttgtca gctccggtac cgaagccacc atgaccgcca tccgtctggc acgcggcttt
      781 accggccgtg acaaaatcat caagtttgaa ggctgctacc acgggcattc cgacagcctg
      841 ctggtgaaag ccggcagcgg cctgctgacg ttcggcaatc cttcttccgc cggtgtgcct
      901 gccgacttta ccaaacacac tttagtcctt gagtacaaca acatcgccca attggaagaa
      961 gcctttgctc aaagcggcaa tgacattgcc tgtgtgattt tggagccttt tgtcggcaat
     1021 atgaaccttg tccgcccgac cgaagccttc gtcaaagccc tgcgcgaatt gaccgaaaaa
     1081 cacggcgcgg tgttgattta cgacgaagtg atgaccggtt tccgcgtcgc gctcggcggc
     1141 gcacaatcgc tgcacggcat catgcccgac ctgaccacga tgggcaaagt catcggcggc
     1201 ggtatgccgc ttgccgcgtt cggcgggcgc aaagacatca tggaatgtat ttccccgctg
     1261 ggcggcgtgt atcaggcagg tacattatca ggcaacccga ttgccgtcgc cgcaggcttg
     1321 aaaacgctgg aaatcattca gcgcgaaggg ttttatgaaa acctgacggc gcgtaccgaa
     1381 cagttggttc aaggttttca gacggcagcg gatgcggcgg gcatcgagtt caccgccgac
     1441 agcgtgggcg gtatgttcgg tctgtatttc gccgcacacg cgccgcgaaa ttatgccgat
     1501 atggcgcgtt ccaatattga aggcttcaaa cagtttttcc acgggatgct cgaccgcaat
     1561 gtcgccttcg gcccgtccgc ttatgaagca ggtttcgttt ccgccgcgca tacgccggtt
     1621 ttgattgatg aaacggttgc ggttgcggtt gaagtgttca aggcgatggc tgcatgatgt
     1681 tttgacggac agggtttctc tgttcgattt gtttggcaga ttgaagtaag aatgcacacc
     1741 gccgtcattc ccgcgcaggc gggaatccag acctttcagt ttctgtaatg attgaaaata
     1801 acgggccgaa atcagccgcg cccggcagga agccgccgcc acctgaaaca atgccgtctg
     1861 aagccttcag acggcatttt atcggaagcg catcagaaaa aacggatagc gacatatgcg
     1921 gtaaaagcaa gggcaattac atagataaac ccctttataa attcaaaagt ctcatctata
     1981 tcgctatatt tttctttcag ttcgtcagat tttcgataac gtttcatttt caagaccact
     2041 tcatgaccaa caagcccgac cttccggatt cccgcctgcg cgggaatgac gggcgtgtat
     2101 atttttgatt tcaatctgct gtaaaaatgc cgtctgaaat gcaggatttg cgcttcagac
     2161 ggcatttttt gtttcttatt gcgcttcggc tctcggggcg gaaatcagaa agtgttcgcg
     2221 gtagtggcgc atttcttcga tgctctccaa aatgtcgtcc aatgccttgt gcgaaccgcg
     2281 tttgacgacg cttttggcaa cgggcggatt ccagcgtttg gcaagctctt tcagtgtgga
     2341 aacgtcaagg ttgcggtagt ggaagtagtt ttccagtttc ggcatatatt tgaccataaa
     2401 acgccggtct tggtggatgg aattgccgca catcggcgtg gcgcgtccgg gtacccattc
     2461 cgacataaag tccagcagtt tctgttcgac ttcggcttcg gtatgcgacg attcgcgtac
     2521 gcgctgtgtc agccccgtcc tgccgtgtgt ggcggtgttc cactcgtcca tattgtcgag
     2581 cagctcgtca ctttggtgga cggcgtaaac ttcggattgc gccaatacat tcaaatccga
     2641 gtcggtaata atcatcgcga cttcgataat gcggtcggtt tcgggattaa gccccgtcat
     2701 ttccatatcg agccagcaga ggttgttttt gtcctgcacg gtgtttcctt tccgtttcag
     2761 cgttttgtcc cgctcaagcg cgcccagctt tcttcggttt ccgccgggtc gagatcgaac
     2821 cattggctgt aaatgccgcc gagttcttcg gcttgttcgt ccaacaaacc ggataacacg
     2881 atgcggccgc cttgtttggt gcgggcggcg agcatttcgc cgagcatacg taaagggttg
     2941 gcgaggatgt tggcgacaac tacgtcgaat tgcccttgag gcagaccgtc gggcaggaag
     3001 aattgtgcat cgacgttgtt ctgttcggcg ttgtccttgc cggcgcggac ggcttgttcg
     3061 tcaatatcca cgccgacggc agaacctgcg ccgagtttga gggcggcgat ggtcaggatg
     3121 cccgaaccgc agccgtagtc gaggacgctt tcgccgtttt tgagttgcgt atccagccat
     3181 ttgaggcaga ggcgcgtggt cgggtggctg ccggtgccga aggcgaggcc gggatcgagg
     3241 cgtaggttga cggcagtgcc ttcgggggct tcgtgccaag agggggtaat ccacaggcgt
     3301 tcggaaattc ggatggggtc gaattgcgct tgggtcaggc gtacccagtc ttggtcttcg
     3361 aggatttcgc cggtgtatgc caagtctttt aatccgcatt cttgtgcggc ggtttggatg
     3421 atggcggcgg cttcgtcgtg ttcgccgaac agggcgatga ctttgctctg ctgccagatt
     3481 tgttcggcgg gcataccggg ttcgccgaaa atcgcctgtt cgttttgcgt gccggcgtag
     3541 gcatcttcga tggcggcgga gagtgcgccg tgttccatca gcgcgtcggc gaggcgttcg
     3601 gcgacggcat cgttgacgtt gacggtgatt tgttggtagg gcataacggg ctttcttaac
     3661 agaccttcgg aatagggtcg tactgtataa ggggatgggt gtgtaacgga aatgccgtct
     3721 gaaaacggac aaactgtttc agacggcata tcggcggcgg tttacttgtc ctgtttggct
     3781 ttgcgatctt ccagccagtg ttccaaatag tggatgctga caccgccttt ttggaaaccc
     3841 gcatcggcga acaggtcgcg gtgaagcggc gtattggttt tgatgccggt taccgccagc
     3901 tcggcgagtg cgacgcgcat tttcgccatt gcctgttcac gcgttttgcc gtgtacgcag
     3961 attttgccga tcaggctgtc gtagtacggt gggatgcggt agccttggta aatgtggctg
     4021 tccacgcgga taccgaagcc gccgggcagg tggcagcttt caatcgggcc cgggctgggg
     4081 atgaagttgt atgggtcttc ggcgttgata cggcactcaa acgcgtggcc ttcgacttga
     4141 atatcctttt gtttgtattg caaaggcagg ccggcggcga tgcggagttg ctcctgcacg
     4201 atgtccacgc cggtgatgag ctcggtaacc ggatgctcga cctgaacgcg cgtgttcatc
     4261 tcgataaaga aaaattcgcc gtcttcgtat aaaaactcaa acgtacccgc gccccggtag
     4321 ccgatgcgtt tgcaggcatc ggcacaggcg ttgccgattt tggcgcgttc tttttcagtg
     4381 atgaacggag ccggtgcttc ctcgatgact ttttggtggc ggcgttgcag cgaacagtcg
     4441 cgctcggcaa ggtagatggc gttgccgtgt tcgtcggcaa tcacttggat ttcgacgtgg
     4501 cgcggacgtt gcaaatagcg ttccatgtaa accatcgggt tgccgaatgc cgcgcccgct
     4561 tcggccttgg tcatttcgac agattggagg aggtcttctt ttttctcgac cacgcgcata
     4621 ccgcgaccgc cgccgccacc ggaggctttg ataatgacgg gataaccgac tttatcggcg
     4681 attttgagga tttcttcgcc gtcgtcgggc aatgcgccgt cagaaccggg gacgcagggt
     4741 acgcctgccg ctatcatcgc gtgtttggcg gagactttgt cgcccatcag gcggatggtg
     4801 tcgggtttcg ggccgataaa ggtaaagccg gactgctcga cctgttcggc gaaatcggcg
     4861 ttttcggcaa ggaaaccgta gcccggatgg acagcgtccg cgcagcttac ttcggcggcg
     4921 gcgataatgg cggggacgtt aaggtagctt tgcgcggaag cggcagggcc gatgcacacg
     4981 gattcgtcgg cgagtttgac gtgcaggctg tctttgtcgg cctcggaatg cacggcgacg
     5041 gtggcaatgc ccatttcgcg gcaggcacgg agtacgcgta atgcgatttc gcctcggttg
     5101 gcgattaaaa cttttttcag catgatgact gttcctgtgg ttccggcaac ccgtccgtaa
     5161 aaaaaaaggt gcgggaacgc cgaaggggga aagcatttca gacggcatcg gaagtttatg
     5221 ccgtctgaaa acaggattat ccgatgatga agagcggttc gccgaattcg acgggcgtac
     5281 cgttttcgac caaaatttct ttgaccgtgc cggatttttc ggcttcgatt tcgttcatca
     5341 gcttcatcgc ttcgatgatg cacagcgtgt cgccggcttt aacctgttgg ccgacttcga
     5401 caaaaggcgc ggcattcggg ccgggtgcgc ggtagaacgt gccgaccata ggcgattttt
     5461 gcgcgtcgga caaatcgcgg gcggcaggtg cggcggcggg cgcggatgcc gcaacgggtg
     5521 cggcggcagg cgttacggcc ggcgcggcgg caggtacggg cgcggcgtaa acgggagctg
     5581 ccgcagctgc aatggtacgg gtgatgcgga ctttttcctc gccttcggtt acttcgattt
     5641 cggcgatacc cgattcttca accaaatcaa tcagtttttt taatttgcgc aaatccattt
     5701 ctgttccttt aaaggctgcc ggtgttccgg cgggttgttg cgttggtttt tcagaaaaca
     5761 gccagcttgc ggcaacggcg caaaccgggc ggaatggtgt tcggatacgg ttttcctgtg
     5821 gtggaaaaca accgtgagaa tgtgtttatt ttcccgaagt tgtcggtaaa tgtccagtaa
     5881 aatatcaaaa aacggcggtt tttgggggaa atgtgcagga agtttgattt tgcgcacaag
     5941 atgccccggg tcaagagtgt ttattctaat ctgttggttt ttcgggcaaa gatgccgtct
     6001 gaaaaagggc taaagtgcgt ataatggcgg cttgcccgaa cgagagtgta aaaatggata
     6061 tttcagattt tgattttacc ctgcccgaac acctgattgc ccagcatccg cccgaagtgc
     6121 gcggcagcag ccaccttttg gtcgcgctgc ccgatatgcc gctgcaagac cgggtgttcg
     6181 gcgatttgcc ggattatgtc gaggcgggcg acgttttggt gttcaacaac accaaagtca
     6241 tgaaggcgcg gctgtttggg cagaaagaca gcggcggcag gatcgaagcc ctgatcgagc
     6301 gtgttttaga cagtcatacc gcactggcgc acatccgttc gtccaagtcc cccaagcccg
     6361 gtatggggct ggtgtttgaa ggcggcatcc gcgccgtgat ggtcgggcgt gagggcgaac
     6421 tgttctgcct gcgttttgaa ggcggagaga ccgtttacga acttttggaa cagaacggac
     6481 acctgcccct gccgccttat atcgaacgtg ccgccgatgc ggacgacgac agccgttatc
     6541 aaaccgttta tgccaaatat cagggcgcgg tcgccgcgcc gacggcgggc ctgcatttta
     6601 cggaagaact tttgcgccgt ctgaaagaca aaggcgcggt aaccgcagaa gtaaccctgc
     6661 acgtcggtgc gggaacgttc caacccgtgc gcgtcgataa aatcgaagaa cacaaaatgc
     6721 acagcgaatg gtttgaagtg ccgtctgaaa ccgccgccgc cgttgaggcg gcaaaagccc
     6781 gggggaacaa agtctgggcg gtcggcacga cttccatgcg cgccctcgag tctgccgcgc
     6841 gtgcgacggg tcgtctgaaa gcggggcagg gcgacaccga tattttcatc acgccgggct
     6901 accgttttaa tgttgccgac aggctggtta ccaatttcca tctgccgaaa tcgacgctgc
     6961 tgatgcttgt cagcgcgttt tcgggtatgg gtcatatccg cgccgcgtat cgttatgcgg
     7021 tcgaacgcga ataccgtttc ttcagctacg gcgatgcgat ggttttgggg tggaacgaag
     7081 gggtggtacg ttagaacagc cccaagtcta ttgcgggaag acaagtgccg tctcaacgga
     7141 cgttcggacg gcattctttt gttgatttga atgaaaggaa ctgtatgaca tagattcagg
     7201 cgcgagaggc gagaaactca gtcattgttg atgacgcgca atacgttaaa attcattccg
     7261 ttttggtcgt ataaacatat tacatacgcc atttttttgt attcaaggct gcctgaaaac
     7321 agtgggcaag aatatctaac ccaacgaaaa gctgaaaaag caacaaaaaa taatctgcac
     7381 cttagtcggt tggttagtcc aaccttaggg gtgcagacca ttcaatcttt caggctgcct
     7441 ttgtttgttt ataagataaa tttttagccg aacacttttt ccaagtgtgc ctgataatct
     7501 gccaagtatt tttccacttg tggattttta accacatcgt tacataagaa tgtcggcagg
     7561 cgggtcatac ccaaaaactc gttggctttg tggaagtgca tatacaaaac atcaacgcct
     7621 ttgccttcaa agaaatcgcc ttcgcgggta aacgcctcaa tcggcgcatt ccaagtcagt
     7681 gaaagcatat gttttttgcc ttgcaacaag ccgcctgtgc cgtagccctc agtcggattg
     7741 acgcggtgtc tgccgtcgct ttggtagagt ttgccgtgtc cagcggttaa tacttcgtct
     7801 atgtattttt tcactgtcca aggctcgtgc atccaccagc ccggcatctg ccaaatcaca
     7861 gcatccatcc aaacgaattt ttcgatttct gcctcaacat catagccggc atcaatcacg
     7921 gtttcttgaa cattgtgtcc gagcgcggtc aaaacttctt tcgctttttt gtgaagcgtg
     7981 cggtttaacc cgccgtgaga atgtccaaac gctttgccgc cgtctaataa taaaatattc
     8041 atttgttacc tcgtttgtga attgatgaat gtattgtgcc gttttacttt ataatttaaa
     8101 agtgcaaaaa taagaaaaca cttttgcgtc aaatgaaata atcagatgtt ccaacacacg
     8161 ggatggcaca taaagcaccg ccctgtgtgc cgtcctgatt cggaaggggt tacacccctc
     8221 ccaaataaag tctgatcctg ccgccctaaa gggcggggtt ccaaccgaaa aggaaacacg
     8281 atgaaaacca attcagaaga actgaccgta tttgttcaag tggtggaaag cggcagcttc
     8341 agccgtgcgg cggagcagtt ggcgatggca aattctgccg taagccgcat cgtcaaacgg
     8401 ctggaggaaa agttgggtgt gaacctgctc aaccgcacca cgcggcaact cagtctgacg
     8461 gaagaaggcg cgcaatattt ccgccgcgcg cagagaatcc tgcaagaaat ggcagcggcg
     8521 gaaaccgaaa tgctggcagt gcacgaaata ccgcaaggcg tgttgcgcgt ggattccgcg
     8581 ataccgatgg tgctgcatct gctggcgccg ctggcagcaa aattcaacga acgctatccg
     8641 catatccgac tttcgctcgt ttcttccgaa ggctatatca atctgattga acgcaaagtc
     8701 gatattgcct tacgggccgg agaattggac gattccgggc tgcgtgcacg ccatctgttt
     8761 gacagccgct tccgcgtaat cgccagtcct gaatacctgg caaaacacgg cacgccgcaa
     8821 tctacagaag agcttgccgg tcaccaatgt ttaggcttca ccgaacccgg ttctctaaat
     8881 acatgggcgg ttttagatgc gcagggaaat ccctataaga tttcaccgca ctttaccgcc
     8941 agcagcggtg aaatcttacg ctcgttgtgc ctttcaggtt gcggtattgc ttgcttatca
     9001 gattttttgg ttgacaacga catcgctgaa ggaaagttaa ttcccctgct cgccgaacaa
     9061 acctccgata aaacacaccc ctttaatgct gtttattaca gcgataaagc cgtcaatctc
     9121 cgcttacgcg tatttttgga ttttttagtg gaggaactgg gaaacaatct ctgtggataa
     9181 ttctataaaa acctgtcata aaactgtttt tactcactta ataactttga gaaaaaataa
     9241 ataagataat gtatagccgg cggcaatggc agtctatatg gggagcgtga tagggctgtt
     9301 ccttatgtac ggcttgtgtg tgccaacatc ggctttccaa atgacaaaat gccgtctgaa
     9361 accgtaaaaa aaaaggtttc agacggcatt ttcaacctga ttcaggcatc agttgcggtt
     9421 tttcaaacgc ccgtgcaact cttgaacgct gtacacgccc agatggtcgc ggcttttcgc
     9481 gccttcgctc atcgcttcgc cgccggcagt ggtggtgtat tgcggcacac gttgctgcaa
     9541 tgcgctttgg cggatgatgt ggctgtcgga cacggattgc ggatcgctgg aaacggtatt
     9601 cacgaccagt gcgatttcac cgtttttcag cgcgtcgccg atgtgcgggc ggccttcggg
     9661 gactttattg atggtctgca caatcagccc gtgttcggtc aggtattgcg ccgtgccgcg
     9721 cgtggcgcag atgccgtagc ctaaggcttg gaagtttttg gcggttttaa tgacgcgttc
     9781 tttgtcttct tcgcgcacgg agaggaagat tttgccggtc gggttgaggc gttcgcccgc
     9841 gccgagttgg gctttgtagt aggcttcgcc gaaacttgcg cccacgccca tcacttcgcc
     9901 ggtggagcgc atttccggtc ccaaaatcgt atccacgccc gggaatttga tgaaggggaa
     9961 cacggcttct ttaacggcat agaaatcggg gacgacttct ttttccacgc cttgttcttt
    10021 cagggaaatc cccgccatcg cccgcgcgcc gactttggcg agcggcacgc cggtggcttt
    10081 ggagacgaag gggacggtgc ggctggctcg cgggttcact tccaatacga acactacgcc
    10141 gtcctgcacg gcaaactgca cgttcatcag tccgaccacg cccagcgcgt acgccatggc
    10201 tttggtttgg cggcggattt cgtcttggat ttcttcgctt aaggagtagg gcggcagcga
    10261 gcagccggag tcgccggagt ggatgcccgc ctgttcgacg tgctgcatga tgccgccgat
    10321 aaccacatct ttgccgtctg aaacgcagtc cacatcgact tcaatcgcgt tgttgaggaa
    10381 gaaatcgagc aacacggggc tgtcttcgga aacctgcacg gcttcgcgca tgtatttttg
    10441 caaggcttcg gcagagtgga caatctgcat cgcgcgtccg cccaaaacat aagacgggcg
    10501 cacgaccagc ggatagccga tttcttcggc tttgacgagt gcttcttctt cgttgtgggc
    10561 gatgcggttg ggcggttggc gcagacctaa gtcgttcaac actttttgga agcgttcgcg
    10621 gtcttcggcg gcgtcgatgc tgtcggcgga cgtgccgatg atgtttacgc cgttttcaac
    10681 caacgcgttg gcgagtttga gcggggtttg accgccgtaa tgcacaatca cgccccacgg
    10741 gttttcggtg cggacgattt ccaacacgtc ttccaatgtc agcggctcga aatagaggcg
    10801 gtcgctggtg tcgaagtcgg tggacacggt ttctgggttg cagttgacca taatcgtttc
    10861 aaaaccggat tcgcgcaggg cgagcgcggc gtgaacgcag cagtagtcaa actcgatgcc
    10921 ctgaccgatg cggttcgggc cgccgccgag aatcatcact tttttacggt cggaaggacg
    10981 ggattcgcat tcttcttcgt aagtggagta aagataggcg gtttcggtgg cgaactcggc
    11041 ggcgcaggtg tcaacgcgtt tgtaaaccgg atgcagcttc agcgcgtagc ggtgttcgcg
    11101 cacttctttt tcttttacgc ccaaaagctg cgcgatgcgt ttgtcggaga agcctttgcg
    11161 tttcagacgg cgtagggcgg cgaaatccaa atcactcaaa atgccgtctg aaaccgattt
    11221 ttcttctttc accaagtctt cgatttgcgc caagaaccaa gggtcgatgg cgcagatttc
    11281 gtggatttct tccggcgtga agcccgcgcg gaacgcgtct gccacaaaca gcatgcgctc
    11341 ggggccgggg tttgccagtt cgcggcggat ttccgctttg tcggagcttc tcggattgaa
    11401 gccgcacaag cctgtttcca agccgcgcag ggctttttgg aaactttcct gaatcgtgcg
    11461 tcccatcgcc atcacttcgc ccaccgattt catctgcgtg gtcaggcggt cgtctgcggc
    11521 agggaatttt tcaaacgcaa aacgcgggat tttggttacc acatagtcga tggacggctc
    11581 gaatgacgcg ggggttttgc cgccggtgat gtcgttgcgc aactcgtcca gcgtaaagcc
    11641 gaccgccagt ttcgccgcca ccttcgcaat cgggaagccc gtcgctttgg aagccagcgc
    11701 ggatgaacgg ctcacgcgcg ggttcatctc aatcacaatc atctcgccgt tttcagggtt
    11761 caccgcaaac tgcacgtttg agccacccgt gtccacgccg atttcgcgca atactgccaa
    11821 cgaagcatta cgcatgattt ggtattcttt gtctgtcaat gtttgcgccg gcgcaaccgt
    11881 aatcgagtcg cccgtatgca cgcccatcgg gtcgaagttt tcaatcgagc aaatgatgat
    11941 gcagttatcg ttcttatcgc gcaccacctc catctcgtac tctttccagc cgaggacgga
    12001 ctgctcaatc agcagctcgt gcgtgggcga cgcatcgaaa ccgcgttcgc aaatcgccaa
    12061 aaactcgtct ttattgtagg caatgccgcc gcccgaaccg cccatggtga aagaaggacg
    12121 aatcagggta gggaagccga cctgttcttg cgccgccaaa gcttcgttca tcgtgtggca
    12181 gacaaaagat ttcgggcaag acaaaccgat tttttccatc gcctctttaa agcggccgcg
    12241 gtcttctgcc ttgtcaatcg cgtcttccgt cgcgccaatc agctcgacat tgtattttgc
    12301 cagcacgccg ttgcgtgcca aatccagcgc acagttcagc gcggtctgac cgcccatcgt
    12361 gggcagaatc gcatcaggcc gctccttggc gataatcttc tccaccgtct gccacataat
    12421 cggctcgatg taggtaacat ccgccatttc agggtcggtc atgatggtgg cggggttgga
    12481 gtttaccaaa atgactttat agccttcttc acgcaaggct ttgcaggcct gtgcgcccga
    12541 atagtcaaat tcgcaagcct gaccgataac gatagggccg gcgccgatga taaggatgga
    12601 ttttaggtcg gtacgtttgg gcatgatggg tctttctatg aaatcaaatg tttagctaaa
    12661 aatttattgc tgattttgat tggcttttaa ttgctctacg acagtattgg caatctcttt
    12721 tcctgatggg gctgtaattg tgtaataaaa gctaatactg cctatgaaga aactgataat
    12781 ggctatgata taggctatat aactttgtgt tttcattttt ttgtgatcat gtttgatctg
    12841 atttaattct tttaattcag aaataattgg agttaataaa tgtttttgac tttcagatcc
    12901 atgcgtttcc aaaagttcat ttattcctat tagtgttggt tttagttcgt ctgagcagcc
    12961 atcaaaaggt tcttcttgtt ggatttcctt ttgtaactgt ctaactaaat ttaaatttaa
    13021 ttcatcagtg taatatagtt gcttaataat ttttctggca gcaaaattat taaggtttac
    13081 tgcttctgtc atttgtttta tatcattaag atctaggtta ttgtgattat tttttaatat
    13141 atcaattatt ctagatttaa atttcttttt ctttatattc tctaaatata gctgaattgg
    13201 aataaaaaca attataaaga gcaaaagaaa tatatatgtt agtgccatag cccatgtggc
    13261 aattatattt atcatttatc aatatcttcc atttggttta tatattgtct cataataagc
    13321 aagccgtagc tcgcatgcag ggtgtgtgcg gtacgcacgc gttctccatt ttccctgcaa
    13381 ccccaaccca ccgtgtgcgt gccttgcggc acacacccta ccgatgggtt caaaggtcgt
    13441 ctgaaaaaac gctcgcggtg tttgttacat ctgccttgcg ataaaggctg cctgaaaaat
    13501 tcccgctgtt gatatttcgg gcattctttc tcaaaccgcc tgcaaccgca aaatccgctg
    13561 caacctgccc cctcccccgt gggggagggc tggggagatg gaattctccg aatttcggca
    13621 accttcccca acaccctagc cgcccaaaca caagccttgc ggcttgttgc cctctctctg
    13681 gctctctccc acgggagaga ggacggggcg gctgtagggc taagggttct gcaaggaaac
    13741 aggttgtgca ggttgctttt tgcttttcag acgacctgaa atcaacggta aatccaagtc
    13801 gcttttcaaa ccgcctgcaa ccgcaaaatc tgctgaaacc tactccctcc cccgtggggg
    13861 agggctgggg agagggcatt ctccgaattt cggcaacctt tcctaacact ctaaccgcca
    13921 agacacaagc cttgtggctt gttgccctct ctctagctct ctcccacggg agagaggatg
    13981 gggtggctgt tggggctaag gtttctgtaa actaatcagg ttatgcaggc tgctttttgt
    14041 ttttcagacg gcctttataa atgctcaatt cagtcggctt tatcctatta ttttcaataa
    14101 tccaatcaaa atcagccatc tgctatcgtg cagcttgctt ttccaattct tgcaacacga
    14161 gcaggatttc tgtcagcaca tcgtttgtct gctgtaaaat ctcgtgattc caaaaccgca
    14221 gtacggtaaa gcccaagctg ttgagatatg ccgtccgcgc gtggtcgtat gcggcttgtt
    14281 ctgtgtgctg ccctccgtct gcttcgacaa tcaacttggg cgttacgcac ataaaatcaa
    14341 caatatagtt tcccatcggc tgctggcggc ggaatttata gccgttcaga cgacctgccc
    14401 gcaggtgctg ccacaatttt gcttccgccc cgctcatttc ttggcgcatg gctttggcgc
    14461 gttggcgcaa gatggggttg tcggtggtca agagtttctc gtgcgggttc attttgttgt
    14521 cgtgctgttt gctgccctct ctctaactct ctcccatggg agagaggacg tggtggcggt
    14581 tgggattaag ggttttgcaa ggaaaacagg ttgtgcaggc tgctttttat ttttcagatg
    14641 acctgcaatc aacggttgta aatccaagtt gcttttcaaa ccgcctgtaa ccgcaaaact
    14701 tgctgcaacc taccccctcc cccgtggggg agggctgggg agagggcatt ctccgaatcg
    14761 cggcaatctt tccctaattc cctaactgcc tcaatacaag cctttcggct tgttgccctc
    14821 tctctaactc tctcccacgg gagagaggac ggtgcggctg tagtgattaa gggttttgca
    14881 aggaaaacaa ggaaaacaag ttgtacaggc tgttttttat ttttcaagcg acctgaaatc
    14941 aacgcttgta aatccaagtc gcttttcaaa ctgcctgcaa ccgcaaaatc tgctgcaacc
    15001 taccccctcc cccgtggggg agggctgggg agagggcatt ttccaagttg cggcaatctt
    15061 tcccaaatcc cttagtcttc taaaatacaa gccttacggt tgttgccaaa tgaaacttct
    15121 tccataccca aaacttgcgt gtaaaacgca atggttcggt caatgtcggc aacggtcagt
    15181 accagatggt cgagtgcgct aatcttcatt tcccgctttc tttttcaggc ggcggcagtg
    15241 ccgtctgaaa gccattatgc ccgttttgcc gctttcatat tgccaatgaa tttgtcaaac
    15301 aaatagccga catcttgcgg accggggctg gcttcggggt ggccttggaa gcagaacaca
    15361 ggtttgtcgg tcagctcgat gccttgcaaa gtgttgtcaa acaaggattt gtgggtaatg
    15421 cgtgcgttag cgggcagggt gtcggcatca acggcaaaac cgtggttttg gctggtaatg
    15481 acgactttgc cgctgtccaa atcttgcaca gggtggttcg caccgtggtg gctgaagcgc
    15541 attttcaggg ttttcgcgcc gatagcgagg ctgatgagct ggtgtcccaa gcagatgccg
    15601 aaaatcggtt tgccgctttc catcagtttt tgcacggctt cgatggcgta ggtgcaaggc
    15661 tcggggtcgc cggggccgtt ggacaggaac acgccgtcgg gattgagtgc caacacgtct
    15721 tgtgcgctcg tttgggcggg gacgacggtc aggcggcagc cgcgcgaggc gagcatacgc
    15781 aggatgttgg ttttcacgcc gaaatcgtag gcgacgacgt ggtaaggctg ttcgtcaggg
    15841 gtaacgaaac ccttgcccaa tgcccattca ccttccgtcc attcgtaagt ttccgtgcag
    15901 gaaacttctt ttgccaagtc tttgccgacc atgctgccga acgcggcgat gagttcttgc
    15961 gctttttcga ccgtagcgtc cgcgccggtc agaatcgcac cgccttgcgc gcctttttca
    16021 cgcaacagcg tggtcaggcg gcgggtgtcg atgtcggcga tggcaacggt tttgttgcgc
    16081 accagatagt cgtgcaggct ttcggaggcg cggaagttgc tgtgcaagag cggcaggtcg
    16141 cggataatca aaccggcggc gtaaacgctg cggctttctt catcttcggc gttggtaccg
    16201 gtattgccga tgtgtgggta ggtgagggta acgatttgtt tgcagtagga cgggtcggtc
    16261 aggatttcct gatagccagt catcgaagta ttgaacacga cttcgccgga agtcgaacct
    16321 tcgtaaccga ttgatgtacc gtggaatacg ctgccgtcag cgaggacgag gagggcgggg
    16381 gtgctcatga tgggaatcct gtttttaata aaattcttga caatgccgtc tgaagggact
    16441 tcggcagggg gcatccggtc ggaaaaatcc ggtcaaaaaa aaacacgccg cgcaagatag
    16501 acgcgtaacg tgcttttcgg gcgatatgaa ccctgaaagc aacgtatttt aagacgaaac
    16561 ctacccgatt tcaagcgcaa acgcggcagg cgggctgata tgttcgcgcg aaaacggaaa
    16621 tccgtggcgt aaaaccgccc gctccgcgcc cactatgccc gttacgcatt tccccacgtc
    16681 cgtccggcga aactatggga atacccgaat cgtcaggaat acccgaaccg tcattcccgc
    16741 gcaggcggga atccggactt gtcggcacgg aaacttatcg ggtacaacgg tttcttgaga
    16801 ttttacgt
//
LOCUS       DAWFHY010000045        16492 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.045, whole genome shotgun sequence.
ACCESSION   DAWFHY010000045 DAWFHY010000000
VERSION     DAWFHY010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16492
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.045"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            597..1472
                     /gene="prpB"
                     /locus_tag="WGQ68_001266"
     CDS             597..1472
                     /gene="prpB"
                     /locus_tag="WGQ68_001266"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003779818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HEZ0715058.1"
                     /translation="MSQHSAGARFRQAVKESNPLAVAGCVNAYFARLATQSGFKAIYL
                     SGGGVAACSCGIPDLGITTMEDVLIDARRITDNVDTPLLVDIDVGWGGAFNIARTIRN
                     FERAGVAAVHIEDQVAQKRCGHRPNKAIVSKDEMVDRIKAAVDARVDENFVIMARTDA
                     LAVEGLDAAIERAQACVEAGADMIFPEAMTDLNMYRQFADVVKVPVLANITEFGSTPL
                     YTQSELAENGVSLVLYPLSSFRAASKAALNVYEAIMRDGTQAAVVDSMQTRAELYEHL
                     NYHAFEQKLDKLFQK"
     gene            1558..2712
                     /gene="prpC"
                     /locus_tag="WGQ68_001267"
     CDS             1558..2712
                     /gene="prpC"
                     /locus_tag="WGQ68_001267"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HEZ0715059.1"
                     /translation="MTETTQTPTFKPKKSVALSGVAAGNTALCTVGRTGNDLSYRGYD
                     ILDLAQKCEFEEVAHLLIHGHLPNKFELAAYKAKLKSMRGLPIRVIKVLESLPAHTHP
                     MDVMRTGVSMLGCVHPEREGHPESEARDIADKLIASLGSILLYWYQYSHNGKRIEVES
                     EEETIGGHFLHLLHGKRPSESHIKAMHVSLILYAEHEFNASTFTARVIAGTGSDMYSS
                     ITGAIGALKGPKHGGANEVAYDIQKRYRNADEAEADIRERIGRKEIVIGFGHPVYTIS
                     DPRNVVIKEVARGLSKETGDMRLFDIAERLESVMWEEKKMFPNLDWFSAVSYQKLGVP
                     TAMFTPLFVISRTTGWSAHVLEQRKDGKIIRPSANYTGPEDLAFVEIEER"
     gene            3038..3982
                     /locus_tag="WGQ68_001268"
     CDS             3038..3982
                     /locus_tag="WGQ68_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase family protein"
                     /protein_id="HEZ0715060.1"
                     /translation="MYHKKFDKEILLKIKELYRYDNWHAVLAILYDYLIIIFSIFLSE
                     QSYWFLPLTILLIGSRQRALATILHEASHSALTKNKKFGQILGTYFSGYLIFQSWNSY
                     CVSHVKNHHPKLGTDSDPDYKYYKDSGIFQTYSRIEFFWRFCVSQILCLKLFRNFKYL
                     IKNRLFSDITRTELFKLLLSQLILFVIGTYFVGYKFYVIYWLLPYLTAFQMITWFIEL
                     SEHYPMIQTAKNNLEASRNRFSHAIEHFFTGVHGENYHLIHHLFPAIPFWNLEKAHKI
                     LMKDFDYQEVNKDFGGIFFSKNNNAPMWKVLWSKYEAA"
     gene            3969..4880
                     /locus_tag="WGQ68_001269"
     CDS             3969..4880
                     /locus_tag="WGQ68_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0715061.1"
                     /translation="MKLRDLLMGIFLAVSAALLNATIGIFSKILMEQGLSVQHIAFLK
                     TLTGFVFISILLCRTGFTRQIADISRKKEAILPLLLKVAICAFFGIYTLFFFETTAYQ
                     YGNAANVVVVLMASAAVSALILDSILLDERICISSVVGVGLAVLGIAMISWTGEGSLG
                     LILNAALAGSGYGCFSVLIKKFGLNGGIYLTRILMFFGSIFLFIPSLEGIEDIHWQWS
                     FIPPLLALSLLPTILGFYCTTKALDYLSAAKVQVTELAEPLFAAVLAWLFLNEIPEGR
                     FFVGAILIIAGIVSINGLYRPLLKRIE"
     gene            4971..7577
                     /gene="acnD"
                     /locus_tag="WGQ68_001270"
     CDS             4971..7577
                     /gene="acnD"
                     /locus_tag="WGQ68_001270"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HEZ0715062.1"
                     /translation="MAANQRYRKPLPGTDLEYYDARAACEDIKPGSYDKLPYTSRILA
                     ENLVNRADKVDLPTLQSWLGQLIEGKQEIDFPWYPARVVCHDILGQTALVDLAGLRDA
                     IAEKGGDPAKVNPVVQTQLIVDHSLAVECGGYDPDAFRKNRQIEDRRNEDRFHFINWT
                     KTAFENVDVIPAGNGIMHQINLEKMSPVVQVKNGVAFPDTCVGTDSHTPHVDALGVIS
                     VGVGGLEAETVMLGRASMMRLPDIVGVELNGKRQAGITATDIVLALTEFLRKERVVGA
                     FVEFFGEGARSLSIGDRATISNMTPEFGATAAMFAIDEQTIDYLKLTGRDDAQVKLVE
                     TYAKTAGLWADALKTAVYPRVLKFDLSSVTRNMAGPSNPHARFATADLAAKGLAKPYE
                     EPSDGQMPDGAVIIAAITSCTNTSNPRNVVAAALLARNANRFGLKRKPWVKTSFAPGS
                     KVAEIYLKEAGLLPEMEKLGFGIVAFACTTCNGMSGALDPKIQKEIIDRDLYATAVLS
                     GNRNFDGRVHPYAKQAFLASPPLVVAYALAGSIRFDIENDVLGVADGKEIRLKDIWPA
                     DEEIDAVVAEYVKPQQFRDVYVPMFDTGTAQKAPSPLYDWRPMSTYIRRPPYWEGALA
                     GERTLRGMRPLAILPDNITTDHLSPSNAILAVSAAGEYLAKMGLPEEDFNSYATHRGD
                     HLTAQRATFANPKLFNEMVKNEDGSVRQGSFARVEPEGETMRMWEAIETYMNRKQPLI
                     IIAGADYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLQFKPDTNRH
                     TLQLDGTETYDVVGERTPRCDLTLVIHRKNGETVEVPVTCRLDTAEEVLVYEAGGVLQ
                     RFAQDFLEGNAA"
     gene            8380..9549
                     /gene="prpF"
                     /locus_tag="WGQ68_001271"
     CDS             8380..9549
                     /gene="prpF"
                     /locus_tag="WGQ68_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylaconitate cis-trans isomerase PrpF"
                     /protein_id="HEZ0715063.1"
                     /translation="MPQIKIPAVYYRGGTSKGVFFKRSDLPEAAREAGSARDKILLRV
                     LGSPDPYGKQIDGLGNASSSTSKAVILDKSERADHDVDYLFGQVSIDKPFVDWSGNCG
                     NLTAAVGAFSIEQGLVDKGKIPSDGICTVKIWQKNIGKTIIAHVPMQNGAVLETGDFE
                     LDGVTFPAAEVQIEFLDSADGEGSMFPTGNLVDEIDVPNIGRLKATLINAGIPTVFLN
                     AADLGYTGKELQDDINNDAAALEKFEKIRAYGALKMGLISDVSEAAARAHTPKVAFVA
                     PAADYTASSGKTVNAADIDLLVRALSMGKLHHAMMGTASVAIATAAAVPGTLVNLAAG
                     GGTRKEVRFGHPSGTLRVGAAAECQDGQWTTTKAVMSRSARVMMEGWVRVPEDCF"
     gene            9963..11162
                     /locus_tag="WGQ68_001272"
     CDS             9963..11162
                     /locus_tag="WGQ68_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEZ0715064.1"
                     /translation="MSDQLILVLNCGSSSLKGAVIDRKSGSVVLSCLGERLTTPEAVI
                     TFNKDGNKRQVPLSGRNCHAGAVGMLLNELEKHGLHDRIKAVGHRIAHGGEKYSESVL
                     IDQAVMDELNACIPLAPLHNPANISGILAAQEHFPGLPNVGVMDTSFHQTMPERAYTY
                     AVPRELRKKYAFRRYGFHGTSMRYVAPEAARILGKPLEDIRMIIAHLGNGASITAIKN
                     GKSVDTSMGFTPIEGLVMGTRCGDIDPGVYSYLTSHAGMDVAQVDEMLNKKSGLLGIS
                     ELSNDCRTLEIAADEGHEGARLALEVMTYRLAKYIASMAVACGGVDALVFTGGIGENS
                     RNIRAKTVSYLDFLGLHIDTKANMEKRYGNSGIISPTDSSPAVLVVPTNEELMIACDT
                     AELVGIL"
     gene            11520..12140
                     /locus_tag="WGQ68_001273"
     CDS             11520..12140
                     /locus_tag="WGQ68_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEZ0715065.1"
                     /translation="MAQFFAIHPDNPQERLIKQAVEIVNKGGVVVYPTDSCYALGCKL
                     GDKAAMERILSIRKIDLKHHLTLMCADLSELGTYAKIDNVQFRQLKAATPGPYTFILQ
                     ATKDVPARTLHPKRKTIGLRIPDNAIAQALLGELGEPLLSCTLMLPEDGEPLTDPYEI
                     RERLEHAVDLVIDGGWCGTEPTTVIDMTDGTELVRQGCGDTAVFGL"
     gene            complement(12302..12745)
                     /locus_tag="WGQ68_001274"
     CDS             complement(12302..12745)
                     /locus_tag="WGQ68_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEZ0715066.1"
                     /translation="MYLTQHTDYGLRVLIYTAINDDALVNISTIAGTYGISKSHLMKV
                     VTALVKGGFLHSVRGKGGGLRLAAPPDRINIGSVVRHLEPMQLVECMGENNECLITPS
                     CRLTGILGGAMKSFFTYLDGFTLQDLLNKPTYDLLYEPRIPIAVQ"
     gene            12919..13287
                     /locus_tag="WGQ68_001275"
     CDS             12919..13287
                     /locus_tag="WGQ68_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEZ0715067.1"
                     /translation="MKPLKRHPALIGLSRDHHHSLSLCVRLLRTPEERHRDELEPHFS
                     ELETHFREEEAKFAPIWQNVAPELKQRFEKDHARLRQMMASPEYGNAAWNTAFATTLR
                     DHARFEERELFPAVEPFLPA"
     gene            13332..14486
                     /locus_tag="WGQ68_001276"
     CDS             13332..14486
                     /locus_tag="WGQ68_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HEZ0715068.1"
                     /translation="MKFTKHPVWAMAFRPFYSLAALYGALSVLLWGFGYTGTPDLPGF
                     YWHAHEMIWGYAGLVVIAFLLTAVATWTGQPPTRGGVLVGLTVFWLAARIAAFIPGWG
                     ASASGILGTLFFWYGAVCMALPVIRSQNQRNYVAVFALFVLGGTHAAFHVQLHNGNLG
                     GLLSGLQSGLVMVSGFIGLIGTRIISFFTSKRLNVPQIPSPKWVAQASLWLPMLTAML
                     MAHGVMPWLSAAFAFAAGVIFTVQVYRWWYKPVLKEPMLWILFAGYLFTGLGLIAVGA
                     SYFKPAFLNLGVHLIGVGGIGVLTLGMMARTALGHTGNPIYPPPKAVPVAFWLMMAAT
                     AVRMVAVFSSGTAYTHSIRTSSVLFALALLVYAWKYIPWLIRPRSDGRPG"
     gene            complement(14543..15415)
                     /locus_tag="WGQ68_001277"
     CDS             complement(14543..15415)
                     /locus_tag="WGQ68_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003695549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEZ0715069.1"
                     /translation="MPILNHIALIGVGLIGGSFVLDLKRQGLVRTVTGIDTDRDNLER
                     ALERGVIDRASVAIDADSIGGADLVLIATPVATVPAILTALRPVLPEHTWISDVGSTK
                     SSVIEAFRRCLPDRLHHCIAAHPIAGSDRSGAQAAQFGLFRHRKLIITPHGGEHSDGI
                     ALVEELWHAVGAEIYTMDAQRHDAVFAAVSHMPHLTAFAYVHQILDHPDGQEYLKFAA
                     TGFRDFTRIASGHPAVWADICLANKDSLLQLVQGLGKQLDVLANILTTDDREALYRYF
                     EEAKTTRDRWLDGN"
     gene            15500..16312
                     /locus_tag="WGQ68_001278"
     CDS             15500..16312
                     /locus_tag="WGQ68_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEZ0715070.1"
                     /translation="MDKIRVAAVQMVSGVSPETNVAAMKRLVARAAEQGADWVLLPEY
                     WVLMGANDTDKLALAEPLGGGRFQTALSETAKECGVVLFGGTVPLQSSEAGKVMNTLL
                     VYGRDGVRTGLYHKMHLFGFSGLGERYAEADTISAGREVPHLSAEGVSVAAGICYDVR
                     FPEFFRRQLPFDVLMLPAAFTHTTGKAHWELLLRARAVENQCYVVAAAQGGLHENGRR
                     TFGHSMIVDPWGDVLDVLPEGEGIVTADIDANRLNSVRNRLPALKYRVLDAV"
BASE COUNT         3966 a         4169 c         4399 g         3958 t
ORIGIN      
        1 cttgtcctga tttaaattca atccactata tatttaaatt taattaaata atataaaaat
       61 caaatagata acaaactaca tcggattgct taaaaataat acattgtttt tatgtataaa
      121 atattttata agttttcagg attttgatta ttgaaaattt ttctcaattt cctgttaatt
      181 ttatcggaaa caaacaagtt aaaattaatt cattttaatc atagtattaa aataaaatat
      241 taaaatcatg taatgaacct ccttaaaaat gtttgacatt ttcagtcttg tgttttagat
      301 tagcgaaaaa taaaactaca caatactaca aaggaacatt actatgaaac caattcagat
      361 gttttcccct tttctgaata atccccttgt tttcttcttg tctgcggttt tgccgcataa
      421 ttccgaacgg tctgctgttt ttctttgatt cgttttaaat atcaataaga taatttttcc
      481 catatatttt taatgattgg attgggatgc ccgacgcgtc ggatggctgt gttttgccgt
      541 ccgaatgtga tggaagcctg tccatactga aaaaaagtct ataaaggaga aatatgatga
      601 gtcaacactc tgccggagca cgtttccgcc aagccgtgaa agaatcgaat ccgcttgccg
      661 tcgccggttg cgtcaatgct tattttgcac gattggccac ccaaagcggt ttcaaagcca
      721 tctatctgtc cggcggcggc gtggcagcct gttcttgcgg tatccctgat ttgggcatta
      781 ccacaatgga agatgtgctg atcgacgcac gacgcattac ggacaacgtg gatacgcctc
      841 tgctggtgga catcgatgtg ggttggggcg gtgcattcaa tattgcccgt accattcgca
      901 actttgaacg cgccggtgtt gcagcggttc acatcgaaga tcaggtagcg caaaaacgct
      961 gcggccaccg tccgaacaaa gccattgtat ctaaagatga aatggtcgac cgtatcaaag
     1021 ctgccgtaga tgcgcgcgtt gatgagaact tcgtgattat ggcgcgtacc gatgcgctgg
     1081 cggtagaagg tttggatgcc gctatcgaac gcgcccaagc ttgtgtcgaa gccggtgcgg
     1141 acatgatttt ccctgaagcc atgaccgatt tgaacatgta ccgccaattt gcagatgtgg
     1201 tgaaagtgcc cgtgttggcg aacattaccg agtttggttc cactccgctt tatacccaaa
     1261 gcgagctggc tgaaaacggc gtgtcgctgg tgctgtatcc gctgtcatcg ttccgtgcag
     1321 caagcaaagc cgctctgaat gtttacgaag cgattatgcg cgatggcact caggcggcgg
     1381 tggtggacag tatgcaaacc cgtgccgagc tgtacgagca tctgaactat catgccttcg
     1441 agcaaaaact ggataaattg tttcaaaaat gatttaccgc tttcagacgg tctttcaaca
     1501 aatccgcatc ggtcgtctga aaacccgaaa cccataaaaa cacaaaggag aaataccatg
     1561 actgaaacta ctcaaacccc gaccttcaaa cctaagaaat ccgttgcgct ttcaggcgtt
     1621 gcggccggta ataccgcttt gtgtaccgtt ggccgtaccg gcaacgattt gagttatcgc
     1681 ggttacgaca tcttggattt ggcacaaaaa tgtgagtttg aagaagttgc ccacctgctg
     1741 attcacggcc atttacccaa caaattcgag ctggccgctt ataaagccaa gctcaaatcc
     1801 atgcgcggcc tgcctatccg tgtgattaaa gttttggaaa gcctgcctgc acatacccat
     1861 ccgatggacg tgatgcgtac cggcgtatcc atgctgggct gtgttcatcc tgaacgtgaa
     1921 ggccatccgg aaagcgaagc gcgcgacatt gccgacaaac tgatcgccag cctcggcagt
     1981 atcctcttgt actggtatca atattcgcac aacggcaaac gcattgaagt tgaaagcgaa
     2041 gaagagacca tcggcggtca tttcctgcac ctgttgcacg gcaaacgccc aagcgaatca
     2101 cacatcaaag ccatgcacgt ttcactgatt ctgtatgccg aacacgagtt caacgcttct
     2161 acctttaccg cccgcgtgat cgccggtaca ggctctgata tgtactccag cattaccgga
     2221 gcaatcggcg cgttgaaagg tccgaaacac ggcggcgcga acgaagtggc ttacgatatt
     2281 caaaaacgct accgcaatgc cgacgaagct gaagccgaca tccgcgaacg catcggccgc
     2341 aaagaaatcg tgatcggttt cggtcatccg gtgtacacca tttccgaccc tcgcaacgtt
     2401 gtcattaaag aagtggcacg cggtttgagc aaagaaaccg gcgatatgcg cctctttgac
     2461 attgccgaac gtttggaaag cgtgatgtgg gaagagaaaa aaatgttccc gaatctggac
     2521 tggttctctg ccgtttccta ccaaaaattg ggcgtaccga ccgctatgtt cacaccgctg
     2581 ttcgtaattt cccgtacaac cggttggagc gcacacgttc ttgagcaacg caaagacggc
     2641 aaaatcatcc gtccgagcgc aaactacacg ggccctgaag atttggcgtt tgtggagatt
     2701 gaagaacgct gagacggaac acaaccgtag gagaccacca atcggatttc agacgacgtg
     2761 tagcgcgggc tttgcctgcg gaaaactgaa acccgcctac ggttccgctt ttcagacggc
     2821 ctgttgaaag atgcgagaga gaaggttgtc tgaaaaacca gccgcaacgt ctgccgtcat
     2881 tcccaccccc gcctaagcga ggacaggtcg cggcgggaat ggcggaggca gcgcggcaac
     2941 ttttttcacg gttctgccaa aatatgctat gaaagaatgt tcgtcagcat aatggagatg
     3001 aagccgatat tataactaat acggagaatt gtgggatatg taccataaga aatttgataa
     3061 agaaattctt ttaaaaatta aagaattata tagatacgat aattggcatg ctgtacttgc
     3121 tatattatat gattatttaa taataatttt ttctatattt ttatctgagc agtcatattg
     3181 gtttctccct ttaacgattt tattaatagg aagcaggcaa agggcgttgg caacaatttt
     3241 acatgaagcc tctcattctg ccttaactaa aaataagaag tttggacaaa ttttaggtac
     3301 atatttttct ggatatttaa tttttcaaag ttggaatagt tattgtgttt ctcatgtgaa
     3361 gaatcatcat ccaaagttag ggactgattc ggatcctgat tataagtatt ataaagactc
     3421 aggtattttt cagacatatt caagaattga atttttctgg agattttgtg tctctcaaat
     3481 tttatgtttg aaattattta ggaattttaa atatttaatt aaaaatagat tattttcgga
     3541 tattacaaga acagagttgt ttaagctact tctttctcaa ttaattctat ttgttatcgg
     3601 tacatatttt gtagggtata aattttatgt aatttattgg ttattaccat atttaactgc
     3661 attccagatg ataacttggt ttattgagtt gtccgagcat tatccaatga tacaaactgc
     3721 caaaaataat ttagaagcgt caagaaatag gtttagtcat gcaatagagc attttttcac
     3781 gggggtacat ggagaaaatt atcatctaat acatcattta tttccagcca ttccattttg
     3841 gaatttggaa aaagcacata agatattaat gaaagatttt gattatcaag aggtaaataa
     3901 agattttgga gggattttct tttcgaaaaa caataatgca cctatgtgga aagtattatg
     3961 gagtaaatat gaagctgcgt gatttactga tgggaatatt cttggcagtt tctgcggccc
     4021 ttctgaatgc aaccatcggc atattcagca agatattgat ggagcagggc ttgtctgttc
     4081 agcatattgc atttttgaaa actttgacag gtttcgtgtt tatcagcatt ttgctttgcc
     4141 gtaccggttt taccagacag attgcggata tttcaagaaa gaaagaggca attttgccgt
     4201 tgctgttaaa agtagcaatt tgtgcttttt tcggaattta tacgttgttt ttctttgaaa
     4261 ccacagctta tcaatatggc aatgctgcga atgtagtagt tgtattaatg gcatcggctg
     4321 ccgtatctgc cttgatattg gacagcatac tgttagatga acgtatttgc atttcttcag
     4381 tcgtcggtgt gggtttggca gtattgggga tcgcaatgat ttcttggact ggagaaggaa
     4441 gtttagggtt gattctgaat gccgcactgg cgggctcggg ctacggttgt ttttccgttt
     4501 tgattaagaa attcggccta aacggcggta tttatttgac acggatattg atgttttttg
     4561 gaagtatttt tttgtttatc ccttcattgg aaggtattga ggatatacat tggcaatggt
     4621 cttttattcc gccactcttg gcattgtctt tattgccgac gattttagga ttttattgta
     4681 caactaaagc attggattat ttgagtgctg cgaaggtaca ggtaactgaa ttggccgagc
     4741 cattgtttgc tgccgtactg gcttggttgt ttttgaatga aataccggaa ggacgcttct
     4801 ttgtcggcgc cattctgatt attgccggta ttgtgtctat caatgggctg tatcgaccat
     4861 tgttgaagcg aattgaataa tttttatttc ccaaaatttc agacgacctc ttcaacaaac
     4921 gtcgtctgaa accccaaaac caaaaaaata atagatttag gagaaccgac atggctgcca
     4981 accaacgtta ccgcaaaccg ctgcccggta cggatttgga atactacgac gcgcgtgcgg
     5041 cgtgtgagga catcaagccc ggctcttacg acaagctgcc ttacacgagc cgcattttgg
     5101 cggagaattt ggtcaaccgc gcggacaaag tcgatttgcc gacgctgcaa agctggctgg
     5161 gtcagctgat tgagggaaaa caggaaatcg actttccttg gtatccggcg cgggtggtgt
     5221 gccacgatat tctggggcag accgcgttgg tggatttggc aggtctgcgc gatgcgattg
     5281 ccgaaaaagg cggcgatcct gccaaagtga atccggtggt gcaaacccag ctcatcgtcg
     5341 accactcttt ggccgttgaa tgcggcggct acgaccccga tgccttccgc aaaaaccgcc
     5401 aaatcgaaga cagacgtaac gaagaccgtt tccacttcat caactggacg aaaaccgcct
     5461 ttgaaaatgt ggacgtgatt ccggcgggca acggcatcat gcaccaaatc aatctggaaa
     5521 aaatgtcgcc cgtcgtccaa gtcaaaaacg gcgtggcgtt ccccgatacc tgcgtcggca
     5581 cggattcgca cacgccgcac gtcgatgcgc tgggcgtgat ttccgtgggc gtgggcggat
     5641 tggaagcgga aaccgtaatg ctgggacgcg cgtccatgat gcgcctgccc gatattgtcg
     5701 gcgttgagct gaacggcaaa cggcaggcgg gcattacggc gacggatatt gtgttggcac
     5761 tgaccgagtt tctgcgcaaa gaacgcgtgg tcggggcgtt tgtcgaattc ttcggcgagg
     5821 gcgcgagaag cctgtctatc ggcgaccgcg cgaccatttc caacatgacg ccggagttcg
     5881 gcgcgactgc cgcgatgttc gctattgatg agcaaaccat tgattatttg aaactgaccg
     5941 gacgcgacga cgcgcaggtg aaattggtgg aaacctacgc caaaaccgca ggcttgtggg
     6001 cagatgcctt gaaaaccgcc gtttatcctc gcgttttgaa atttgatttg agcagcgtaa
     6061 cgcgcaatat ggcaggtcca agtaacccgc atgcccgttt tgcgaccgcc gatttggcgg
     6121 cgaaagggct ggcgaagcct tacgaagagc cttcagacgg ccaaatgccc gacggcgcgg
     6181 tcatcatcgc cgcgattacc agttgcacca acacttccaa cccgcgcaac gtcgttgccg
     6241 ccgcgctctt ggcgcgcaac gccaaccgct tcgggctgaa acgcaaaccg tgggtcaaaa
     6301 cctcgtttgc ccccggttcg aaagtggcgg aaatttattt gaaagaagca ggcctgctgc
     6361 ccgaaatgga aaaactcggc ttcggtatcg tcgccttcgc ctgcaccacc tgcaacggca
     6421 tgagtggcgc gctggatccg aaaatccaga aagaaatcat cgaccgcgat ttgtacgcca
     6481 ccgccgtatt atcaggcaac cgcaacttcg acggccgtgt ccatccgtat gcgaaacagg
     6541 ctttcctcgc ttcgcctccg ttggtcgttg cctacgcgct ggcaggcagt atccgtttcg
     6601 atattgaaaa cgacgtactc ggcgttgcag acggcaagga aatccgcctg aaagacattt
     6661 ggcctgccga tgaagaaatc gatgccgtcg ttgccgaata tgtgaaaccg cagcagttcc
     6721 gcgatgtgta tgtaccgatg ttcgacaccg gcacagcgca aaaagcaccc agtccgctgt
     6781 acgattggcg tccgatgtcc acctacatcc gccgtccgcc ttactgggaa ggcgcgctgg
     6841 caggggaacg cacattaaga ggtatgcgtc cgctggcgat tttgcccgac aacatcacca
     6901 ccgaccacct ctcgccgtcc aatgcgattt tggccgtcag tgccgcaggc gagtatttgg
     6961 cgaaaatggg tttgcctgaa gaagacttca actcttacgc aacccaccgt ggcgaccact
     7021 tgaccgccca acgcgctacc ttcgccaatc cgaaactgtt taacgaaatg gtgaaaaacg
     7081 aagacggcag cgtgcgccaa ggctcgttcg cccgcgtcga acccgaaggc gaaaccatgc
     7141 gcatgtggga agccatcgaa acctatatga accgcaaaca gccgctcatc atcattgccg
     7201 gtgcggacta tggtcaaggc tcaagccgcg actgggctgc aaaaggcgta cgcctcgccg
     7261 gcgtagaagc gattgttgcc gaaggcttcg agcgtatcca ccgcaccaac cttatcggca
     7321 tgggcgtgtt gccgctgcag ttcaaacccg acaccaaccg ccataccctg caactggacg
     7381 gtacggaaac ctacgacgtg gtcggcgaac gcacaccgcg ctgcgacctg accctcgtga
     7441 ttcaccgtaa aaacggcgaa accgttgaag ttcccgttac ctgccgcctc gatactgcag
     7501 aagaagtatt ggtatatgaa gccggcggcg tgttgcaacg gtttgcacag gattttttgg
     7561 aagggaacgc ggcttagagg tcgtctgaaa agcaagacgt agcgtgggtc gggttcaaca
     7621 ttttgctcat tcacgtaatt ctcgatatgg caggcatcta ctgtaaatcg tcattcccgc
     7681 gcaggcggga atccagaaag tggaattgag gaaaccttat ttatccgatg agtttctgtg
     7741 cggacaaatc tggattcccg cctgcgcggg aatgacgggg tttaataatc tgccgtatca
     7801 caacacagta gccgtagatt gtggcgaacc ccgacagttt gcggaatcaa acggctttgt
     7861 cggagtggca gcctaatgta cttctggaaa gtgggtgtag cgtgggcttt gcccgcgaaa
     7921 taaaggctga attgacatgg tatagaggat taacaaaaat cgggacaagg cggcgaagcc
     7981 gcagacagta cagatagtac ggaaccgatt cacttggtgc ttcagcacct tagagaatcg
     8041 ttctctttga gctaaggcga gccaacgctg tactggtttt tgttaatcca ctataaattt
     8101 aatccactat actgtaaatc gtcattcccg cgcaggcggg aatccagaaa gtggaattga
     8161 ggaaaccttt ttatccgatg agtttctgtg cggataaatc tggattcccg cctgcgcggg
     8221 aatgacggag tttaataatc tgccgtatca caacacagta gccgtagatt ggggcgaacc
     8281 ccgacagttt gcggaatcaa acggctttgg tcggagtggc agcctaatcc actataaaaa
     8341 tcgtgggcag agcccacgct acataaggag aatctagaaa tgccgcaaat taaaattccc
     8401 gccgtttact accgtggcgg tacatcaaaa ggcgtgtttt tcaaacgttc cgacctgccc
     8461 gaggcggcgc gggaagcggg aagcgcacgc gacaaaatcc tcttgcgcgt actcggcagc
     8521 ccggatccct acggcaagca gatagacggt ttgggcaacg ccagttcgtc caccagcaaa
     8581 gccgtgattt tggacaagtc cgaacgcgcc gatcacgatg tcgattacct tttcgggcaa
     8641 gtttccatcg acaaaccttt tgtcgattgg agcggcaact gcggcaacct caccgccgcc
     8701 gtgggcgcat tctccatcga acagggcttg gtcgataaag gcaagattcc ttcagacggc
     8761 atctgcacgg tcaaaatctg gcagaaaaac atcggcaaaa ccattattgc ccatgtaccg
     8821 atgcaaaacg gcgcagtttt ggaaacaggc gattttgagc tcgacggcgt aacgttcccg
     8881 gcagccgaag tacaaatcga atttcttgat tcagccgacg gcgaaggcag tatgttccca
     8941 accggcaatt tggtcgatga aattgatgtg ccgaatatag gccgtttgaa agccacgctc
     9001 atcaacgcgg gcattccgac cgttttcctg aatgccgccg acttgggcta cacgggcaaa
     9061 gagttgcaag acgacatcaa caacgatgcc gcggctttgg aaaaattcga gaaaatccgc
     9121 gcttacggtg cgctgaaaat gggtctgatc agcgacgtat ccgaagctgc cgcccgcgcg
     9181 cacacgccga aagtcgcctt cgtcgcgccc gccgccgatt acaccgcctc cagtggcaaa
     9241 accgtgaacg ccgccgacat cgatttgctg gtacgcgccc tgagcatggg caaactgcac
     9301 cacgcgatga tgggtaccgc ctctgttgcc attgcgaccg ccgccgccgt gcccggtacg
     9361 ctggtcaacc ttgccgcagg cggcggaacg cgtaaagaag tgcgcttcgg gcatccttct
     9421 ggcacattgc gtgtcggtgc agccgccgaa tgtcaggacg gacaatggac gaccaccaaa
     9481 gcggttatga gccgcagcgc acgcgtgatg atggaaggtt gggtcagggt gcctgaggat
     9541 tgtttttaaa tcgacgtagc gtgggtttgc tcgcgagcca taaaaaggtc gtctgaaaaa
     9601 caagtaaaca tcaaatcact gaccattcct ttcccttgcc ctgtggcgga aggcggcaaa
     9661 tcacaaggaa gaacacggaa accccgataa aagacagctt cccgtattac cgtcattccc
     9721 gcgcaggcgg gaatccagac ctgtcaatat ggaagattgg caggggaaaa taggtttcgt
     9781 gagttctaca ttctggattc ccgccacagc ctgtccttgc gtaggcgggg acggaataac
     9841 gataggaaat gcggcatatg ctttgcccaa agaggccgtc tgacacacac tccgcccaac
     9901 gcccatcctt tttcagacga ccccacacca aaaaacaacc acaaactaca aggagaaaca
     9961 tcatgtccga ccaactcatt cttgttctga actgcggcag ttcatcgctc aaaggtgccg
    10021 ttatcgaccg caaaagcggc agcgtcgtcc taagctgcct cggcgaacgc ctgaccacgc
    10081 ccgaagccgt cattaccttc aacaaagacg gcaacaaacg ccaagttccc ctgagcggcc
    10141 gcaactgcca tgccggcgcg gtgggtatgc tgttgaacga actggaaaaa cacggactgc
    10201 acgaccgcat caaagccgtc ggccaccgca tcgcccacgg cggcgaaaaa tacagcgagt
    10261 ctgttttgat cgaccaggcc gtaatggacg aactcaatgc ctgcattccg cttgcgccgc
    10321 tgcacaaccc cgccaacatc agcggcatcc ttgccgcaca ggaacatttc cccggcctgc
    10381 ccaatgtcgg cgtgatggat acttcgttcc accaaaccat gccggagcgt gcctacactt
    10441 atgccgtgcc gcgcgagttg cgtaaaaaat acgctttccg ccgctacggt ttccacggca
    10501 ccagtatgcg ttacgttgcc cctgaagccg cacgcatctt gggcaaacct ctggaagaca
    10561 tccgcatgat tattgcccac ttaggcaacg gcgcatccat taccgccatc aaaaacggca
    10621 aatccgtcga taccagtatg ggtttcacgc cgatcgaagg tttggtaatg ggtacacgtt
    10681 gcggcgacat cgatccgggc gtatacagct atctgacttc ccacgccggg atggatgttg
    10741 cccaagtgga tgaaatgctg aacaaaaaat caggtttgct cggtatttcc gaactttcca
    10801 acgactgccg caccctcgaa atcgccgccg acgaaggcca cgaaggcgcg cgcctcgccc
    10861 tcgaagtcat gacctaccgc ctcgccaaat acatcgcttc gatggctgtg gcctgcggcg
    10921 gcgttgacgc actcgtgttc accggcggta tcggcgaaaa ctcgcgtaat atccgtgcca
    10981 aaaccgtttc ctatcttgat ttcttgggtc tgcacatcga caccaaagcc aatatggaaa
    11041 aacgctacgg caattcgggc attatcagcc cgaccgattc ttctccggct gttttggttg
    11101 tcccgaccaa tgaagaactg atgattgcct gcgacactgc cgaacttgtc ggcatcttgt
    11161 agccaaaaaa gggacgagtc cgcacaatgc cgtctgaaac cccaaacgcc cgattaggct
    11221 gatgaggatt ttagacggca ttgttcattt ttttgttatc ttgcattttt gtgcggacgg
    11281 tggaatttca tcctgtaaac ataaatattt gtcggaaaac agaaaccctc cgccgccatt
    11341 tctacgaaag caggaaacca gcaacgcaaa gcgacaggga tttgttggaa atgaccgaaa
    11401 ccgaacgaac cggattcccg cctgcgcggg aatgacggga ttttctgttt ttgtggaaat
    11461 gacgggattt tgaatttcgg gcgtacaata cggaaaacat gacgataagg aaaaaaacta
    11521 tggcacagtt tttcgctatt catcccgaca acccccaaga acgcctcatc aagcaggcgg
    11581 tcgaaatcgt caataaaggc ggcgtggtcg tttatccgac cgattcctgt tatgccttgg
    11641 gttgcaaact cggcgataag gcggcgatgg aacgcatact ctccatccga aaaatcgatt
    11701 tgaaacacca cctgaccctg atgtgcgccg atttgagcga gttgggcaca tacgccaaaa
    11761 ttgacaacgt acagtttcgt cagcttaaag ccgccacacc cgggccttat acttttattt
    11821 tacaggcgac gaaggatgtg ccggcgcgca cgctgcaccc gaaacgcaaa accatcgggc
    11881 tgcgtattcc cgataatgcc attgcacaag ccctgctggg ggaattgggc gagccgcttt
    11941 taagctgcac cctgatgctg cccgaagacg gcgaaccatt gaccgatcct tatgaaatcc
    12001 gcgagcgttt ggaacacgcc gtcgatttgg tgattgacgg cggctggtgc ggaaccgagc
    12061 cgaccaccgt catcgacatg accgacggta cggaattggt gcgccaaggt tgcggcgata
    12121 cggcggtgtt cggtttgtag ggaaaccgat gccgtctgaa gcatcggctg ttcagacggc
    12181 attgcgcgcc ttgccggcgg cagtccgaaa tgccggcgcg tatcgcgctc ggtcggaata
    12241 tccgtttgaa acggcaaaca aaatgccgtc cgaacgcggg ttcggacggc attttgatgc
    12301 attactgcac cgcaatcgga attctcggtt cgtagagcag gtcgtaggtc ggcttgttga
    12361 gcaggtcttg gagcgtgaaa ccgtccaaat acgtgaaaaa cgacttcatc gcgccgccga
    12421 gtatgcctgt cagacggcag gacggtgtga tcaggcattc gttgttctcg cccatgcact
    12481 cgaccagctg catcggttcg aggtggcgga cgaccgagcc gatgttgatg cggtcgggcg
    12541 gtgcggcaag ccgcagaccg ccgccttttc cgcgcacgct gtggaggaag ccgcctttga
    12601 ccagcgcggt aacgactttc atcagatggc ttttggaaat gccgtaagtt ccggcgatgg
    12661 tactgatgtt gaccagcgca tcgtcgttga tggcagtgta gataaggacg cgcagcccgt
    12721 agtccgtatg ttgtgtcaaa tacatgattt tctcggtatg gatcgttatt cttatcggta
    12781 cggtttaagg ttcacggaca ataccttaat ggttgaaacc ctgtccgtcg gggcggtaga
    12841 atgcagcctg tctgcggcgg tatgccgtct gaaacatccg cgctaccgtt tgataatttg
    12901 ttattgtaac tcaaaatcat gaaaccgttg aaacgacatc ccgcccttat cgggctttcg
    12961 cgtgaccacc accattcgct ttccctgtgc gtgcgtctgt tgcggacgcc ggaagaacgg
    13021 catcgggacg aactcgaacc gcatttttcc gaattggaaa cccattttcg cgaagaagaa
    13081 gccaagtttg ccccaatttg gcagaatgtc gcccccgaat tgaaacaacg tttcgagaaa
    13141 gaccacgccc gactgcggca gatgatggca agccccgaat acggtaacgc ggcgtggaat
    13201 accgcttttg ccacaaccct gcgcgatcac gcgcgctttg aagaacgcga gctgtttccc
    13261 gccgttgaac cgtttttgcc ggcatgattc cgttttgcgg caaatatatt aatgataaac
    13321 aaggaacaca catgaaattt accaagcacc ccgtctgggc aatggcgttc cgcccgtttt
    13381 attcactggc ggctctgtac ggcgcattgt ccgtattgct gtggggcttc ggctacacgg
    13441 gcacgccgga tctgcccggt ttttactggc acgcgcatga gatgatttgg ggttatgccg
    13501 gcctcgtcgt catcgccttc ctgctgaccg ccgtcgccac ttggacgggg cagccgccca
    13561 cgcggggcgg cgttctggtc ggcttgactg tcttttggct ggctgcgcgg attgccgcct
    13621 ttatcccggg ttggggtgcg tcggcaagcg gcatactcgg tacgctgttt ttctggtatg
    13681 gcgcggtgtg catggctttg cccgttatcc gttcacagaa tcaacgcaac tatgttgccg
    13741 tgttcgcgct gttcgtcttg ggcggcacgc atgcggcgtt ccacgtccag ctgcacaacg
    13801 gcaacctagg cggactcttg agcggattac agtcgggctt ggtgatggtg tcgggtttta
    13861 tcggtctgat tggtacgcgg attatttcgt tttttacgtc caaacggttg aatgtgccgc
    13921 agattcccag tccgaaatgg gtagcgcagg cttcgctgtg gctgcccatg ctgaccgcca
    13981 tgctgatggc gcacggcgtg atgccttggc tgtcggcggc tttcgcgttt gcggcaggtg
    14041 tgatttttac cgtgcaggtg taccgctggt ggtataaacc cgtgttgaaa gagccgatgc
    14101 tgtggattct gtttgccggc tatctgttta ccggattggg gctgattgcg gtcggcgcgt
    14161 cttatttcaa acccgctttc ctcaatctgg gtgtgcatct gatcggggtc ggcggtatcg
    14221 gcgtgctgac tttgggcatg atggcgcgta ccgcgctcgg tcatacgggc aatccgattt
    14281 atccgccgcc caaagccgtt cccgttgcgt tttggctgat gatggcggca accgccgtcc
    14341 gtatggttgc cgtattttct tccggcactg cctacacgca cagcatacgc acctcttcgg
    14401 ttttgtttgc actcgcgctt ttggtgtatg cgtggaagta tattccttgg ctgattcgtc
    14461 cgcgttcgga cggcaggccc ggttgagaca aaccgccgca gatttcgggt ctgggcggtt
    14521 tgcttttcag acggcagggc ggtcagttgc cgtccagcca gcggtcgcgt gtggttttgg
    14581 cttcttcaaa atagcggtac agggcttcgc ggtcgtcggt ggtcaggatg tttgccaaaa
    14641 cgtccaactg tttgcccaag ccttgaacca gttgcagcag gctgtctttg ttggcaaggc
    14701 agatgtccgc ccacacggcg ggatggccgg aggcgatgcg ggtgaagtcc cgaaagcccg
    14761 tggcggcgaa tttcagatat tcctgtccgt cggggtggtc gagaatctgg tggacatagg
    14821 cgaaggcggt caggtggggc atatgggaga cggcggcgaa aaccgcgtcg tggcgttgcg
    14881 cgtccatcgt ataaatttcc gcaccgaccg cgtgccacag ttcttctacc aaggcaatgc
    14941 cgtctgaatg ttcgccgccg tgtggcgtga tgatgagttt tctgtggcgg aacagcccga
    15001 actgcgcggc ttgcgcaccg cttctgtccg aaccggcaat cgggtgggcg gcgatgcagt
    15061 ggtgcagacg gtcgggcaga cagcggcgga aggcttcgat gaccgaagat ttggtgctgc
    15121 cgacatcgga aatccaagtg tgttccggca aaacggggcg cagcgcggtc aaaatggcgg
    15181 gaacggtggc gacgggcgtg gcaatcagta ccaagtccgc accgccgatg ctgtccgcgt
    15241 cgatggcaac ggaagcccgg tcaatcacgc cgcgttccaa tgcacgttcg aggttgtcgc
    15301 ggtcggtgtc gataccggta acggtgcgga cgagtccctg ccttttgagg tcgagaacga
    15361 acgaaccgcc gatcagccct acaccgatga gggcaatatg gttcaaaatg ggcatttgtg
    15421 taaacggttt tcgcaaagta ccgtcatggt agcctatcgg cggaatatgc cgcaaggtcg
    15481 gcaggaaaaa ggagaagaaa tggacaaaat cagagttgcc gccgtgcaga tggtgtcggg
    15541 cgtgtcgccg gaaaccaacg tcgctgccat gaaacgcctg gtcgcacggg cggcggagca
    15601 gggggcggat tgggtgctgc tgcccgaata ttgggtgctg atgggcgcaa acgataccga
    15661 caaactcgcg cttgccgagc ctttgggcgg cggacgcttt cagacggcat tgagcgaaac
    15721 ggcgaaagaa tgcggcgtgg tgctgtttgg cggaactgtg ccgctgcaaa gcagtgaggc
    15781 aggcaaggtg atgaatacac tgttggtgta cggacgggac ggcgtaagaa cgggtctgta
    15841 ccacaaaatg cacctcttcg gtttttctgg tttgggcgaa cgctatgccg aagccgatac
    15901 catcagcgca gggcgggaag tgccgcactt gtcggcagaa ggcgtgtcgg tggcggcggg
    15961 catttgttac gatgtccgct ttcccgaatt tttccgccgc cagctgccgt ttgacgtatt
    16021 gatgctgccc gctgcgttta cgcacacgac gggcaaggcg cattgggagc tgctgctgcg
    16081 cgcgcgtgcc gtcgaaaacc aatgttacgt cgtggcggcg gcacagggcg gtttgcacga
    16141 aaacggacgg cgcacgttcg gacacagcat gattgtcgat ccgtggggcg acgtattgga
    16201 cgtattaccc gaaggcgaag gcatcgttac ggcagacatc gatgccaacc gcctgaacag
    16261 cgtccgcaac cgcctgcccg ccttgaaata ccgggttttg gatgccgtct gaaggttcag
    16321 acggcatcgg tgccggggaa tcagcgggct gtaggatggg cttcagcctg ccatttccat
    16381 caatcccaca tttctaccgt tttcatcgaa tccatcgaat ccgccccttt cgacgaccca
    16441 gtcctacgca actgaaccgt cattcccacg gaagtgggaa tctagaatct cg
//
LOCUS       DAWFHY010000046        16432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.046, whole genome shotgun sequence.
ACCESSION   DAWFHY010000046 DAWFHY010000000
VERSION     DAWFHY010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.046"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            855..5555
                     /gene="iga"
                     /locus_tag="WGQ68_001279"
     CDS             855..5555
                     /gene="iga"
                     /locus_tag="WGQ68_001279"
                     /EC_number="3.4.21.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IgA-specific serine endopeptidase
                     autotransporter"
                     /protein_id="HEZ0715071.1"
                     /translation="MKTKRFKINAISLSIFLAYALTPYSEAALVRDDVDYQIFRDFAE
                     NKGKFFVGATDLSVKNKQGQNIGNALSNVPMIDFSVADVNKRIATVVDPQYAVSVKHA
                     KAEVHTFYYGQYNGHNDVADKENEYRVVEQNNYEPHKAWGASNLGRLEDYNMARFNKF
                     VTEVAPIAPTDAGGGLDTYKDKNRFSSFVRVGAGRQLVYEKGAYHQEGNEKGYDLRDL
                     SQAYRYAIAGTPYKDINIDQTMNTEGLIGFGNHNKQYSAEELKQALSQDALTNYGVLG
                     DSGSPLFAFDKQKNQWVFLGTYDYWAGYGKKSWQEWNIYKKEFADKIKQRDNAGTIKG
                     NREHHWNITSGTNSKIGSTAVRLANNERDADNGQNVTFEDNGTLVLDQNINQGAGGLF
                     FKGDYTVKGANNDITWLGAGIDVTDGKKVVWQVKNPNGDRLAKIGKGTLEINGTGVNQ
                     GELKVGDGTVILNQKADSNQKVSAFSQVGIVSGRGTLVLNSSNQINPDNLYFGFRGGR
                     LDANGNDLTFEHIRNVDEGARIVNHNTGHASTITLTGKSLITNPNSLSVHSIQNDYDE
                     DDYSYYYRPRRPIPQGKDLYYKNYRYYALKSGGSVNAPMPENGVTENNDWVFMGYTQE
                     EAKKNAMNHKNNQRISGFSGFFGEENGKGHNGALNLNFNGKSAQNRFLLTGGTNLNGK
                     ISVTQGNVLLSGRPTPHARDFVNKSSARKDARFSKNNEVVFEDDWINRTFKATEITVN
                     QSASFSSGRNVSDITANITATDNAKVNLGYKNGDEVCVRSDYTGYVTCNTDNLSDKAL
                     NSFDATQINGNVNLSQNAALTLGKAALWGQIQGQGNSRVSLNQHSKWHLTGDSQVHNL
                     SLADSHIHLNNASDAQSANKYHTLKINHLSGNGHFHYLTHLAENLGDKVLVKESASGH
                     YQLHVQDKTGEPNQEGLNLFDASSVRDRSHLSVSLANHHVDLGALRYTIKTENGITRL
                     YNPYAENRRRVKPAPSPATNTASQAQKATQTDGAQIAKPQNIVVAPPSPQANQAEEAK
                     RQQAKAEQVKRQQAEAEKVAHQKAEEAKRQQDALARQQAEQERQRLEAERQAAEIAKQ
                     KAEAEEAKRRAAEIAEQKAAAEEAKRQAAELARQQEEARKAAELAAKQKAETERKAAE
                     IAEQKAEAEREAAELAKQKAEEEGRQAAQSQPKRRNRRAIPPELSSDATTRALPRIAR
                     NSNPDASDYEEIPLDALEDEDVSESVDTSDKQPQDNTELHEKVETVSLQPRAAQPRAQ
                     AAAQPQAQAVAQADAVSTNTNSALSDAMASTQSILLDTGASLTRHIAQKSRADAEKNS
                     VWMSNIGYGRDYASAQYRRFSSKRTQTQIGIDRSLSENMQIGGVLTYSDSQHTFDQAS
                     GKNTFVQANLYGKYYLNDAWYVAGDIGAGSLRSRLQTQQKANFNRASIQTGLTLGNTL
                     KINQFEIVPSAGIRYSRLSSADYKLGNDSVKVSSMSVKTLTAGLDFAYRFKVGNLTVK
                     PLLSAAYFANYGKGGVNVGGNSFAYKADNQQQYSAGAALLYRNVTLNVNGSITKGKQL
                     EKQKSGQIKIQIRF"
     gene            complement(5733..6935)
                     /gene="trpB"
                     /locus_tag="WGQ68_001280"
     CDS             complement(5733..6935)
                     /gene="trpB"
                     /locus_tag="WGQ68_001280"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEZ0715072.1"
                     /translation="MKNYHAPDEKGFFGEHGGLYVSETLIPALQELADAYKAAKNDPE
                     FWEAFRHDLKHYVGRPSPVYHAARLSEHLGGAQIWLKREDLNHTGAHKVNNTIGQALL
                     AKRMGKKRVIAETGAGQHGVASATVAARFGMTCDVYMGADDIQRQMPNVFRMKLLGAN
                     VVGVESGSRTLKDAMNEAMREWVARVDDTFYIIGTAAGPAPYPEMVRDFQCVIGNEAK
                     AQMQEAIGRQPDVAVACVGGGSNAIGLFHPYIGEENVRLVGVEAGGLGVNTPDHAAPI
                     TSGAPIGVLHGFRSYLMQDENGQVLGTHSVSAGLDYPGIGPEHSHLHDIKRVEYTVAK
                     DDEALEAFDLLCRFEGIIPALESSHAVAWAVKNAPKMGKDQVILVNLSGRGDKDINTV
                     AKLKGIKL"
     gene            complement(7018..7707)
                     /locus_tag="WGQ68_001281"
     CDS             complement(7018..7707)
                     /locus_tag="WGQ68_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase"
                     /protein_id="HEZ0715073.1"
                     /translation="MLFEGLPCLAVWCKNRLSAVLPVGGLNVSENLLEIETHPFDPVL
                     PPKAAVMMMGTFPPKEDKRAMQFHYPNFQNDMWRVYGLVFFNDAAHFQRLSEKAFDAE
                     KIKAFLHERGIASCPTVLKAVRQHGNASDKFLKVVETVDLAAVLAKIPECRHICTTGG
                     KATEILLDIQGGGIKMPKTGETVPFPFAGRDLTLTRLPSTSRAYPLSLAKKAAAYRAF
                     FEMAGLCEKQL"
     gene            7904..8683
                     /gene="rsmA"
                     /locus_tag="WGQ68_001282"
     CDS             7904..8683
                     /gene="rsmA"
                     /locus_tag="WGQ68_001282"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEZ0715074.1"
                     /translation="MKEHKARKRFGQNFLQDTRIISDIVNAVRPQADDVVIEIGPGLA
                     AITEPLAKKLNRLHVVEIDRDIVCRLKTLPFADKLVIHEGDVLQFDFNGIEGKKKIVG
                     NLPYNISTPLLFKLAEVADDVVDMHFMLQKEVVERMVAAPKSNDYGRLGVMLQYFFDM
                     ELLIDVPPESFDPAPKVDSAVVRMIPVKHRIGKADDFEHFAKLVKLAFHQRRKTIRNN
                     LKELATDEDLQAAGINPQDRAEHIAPEKYVALSNYLAGKAV"
     gene            8856..9584
                     /locus_tag="WGQ68_001283"
     CDS             8856..9584
                     /locus_tag="WGQ68_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEZ0715075.1"
                     /translation="MIKFKNVHKHFKDLHVINGVNLEIKKGEVVVVCGPSGSGKSTLI
                     RTVNQLESIESGEIWVDGVNVADPKTDLNKIREEVGFVFQSFNLYPHLTVLENITLAP
                     MKVKGQNAEQAEKKAMELLERVGLAHKKDAFPSQLSGGQQQRVAIARGLAMEPRVMLF
                     DEPTSALDPEMVGEVLKVMKDLAESGMTMMCVTHEMGFAREVADRVIFVDKGQILEDE
                     TPEAFFTNPKHERAKQFLQQVMTH"
     gene            9657..10016
                     /locus_tag="WGQ68_001284"
     CDS             9657..10016
                     /locus_tag="WGQ68_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715076.1"
                     /translation="MYFVDRTAVVLKPTVRFLEWLKSTDENMPDLTIEQLRANCSVFL
                     VPQFDEPEAVVSYFDERYRQIFEAELAGWDIDKDKWPQDMGLKAFWEFFDIEIHDMVL
                     DMEEADLNITPVFDNMM"
     gene            10021..10467
                     /locus_tag="WGQ68_001285"
     CDS             10021..10467
                     /locus_tag="WGQ68_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FolI"
                     /protein_id="HEZ0715077.1"
                     /translation="MRAFQTALRPSRILNILTVSLHSASLAVCLTWFYGRMMWFGLAA
                     LAASYAYSLRITNLKHRHAITAITIDRDGQAEIVSGKEKTAKAAALSGSSMVTPYALF
                     LQWDTGGKTVRHCITHDMADPESYRRLKVWVLWRQPKKTAETDTPS"
     gene            10499..11773
                     /gene="folC"
                     /locus_tag="WGQ68_001286"
     CDS             10499..11773
                     /gene="folC"
                     /locus_tag="WGQ68_001286"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEZ0715078.1"
                     /translation="MKTLQDWLSHLETAHSGGLIDMGLERVGEVKKRMKLTPQCPVVV
                     VAGTNGKGSVCAYLTQIYKQAGYKTGTLTSPHLLRYNERIAINAEPVSDDTIIASFER
                     IEAARGEISLTYFEFNTLAAVDIFMREQVDVMILEVGLGGRLDAVNAFDGDCAVVTSV
                     DFDHQAFLGDTVEQVGFEKAGVFRGGKPAICGQNPAPASLLSHAEAIGAKLLMVQRDF
                     EFHALENIQWNYRFRPQHSDSPARNRNSLPFPALRGAYQLSNAACALTVLECLNDKLP
                     VDIGAIKRGLLLVENPGRFQVLPGRPLTVLDVGHNPHAARALRRSLINLAFAQKRTAV
                     FSMLSDKDIDGVLEIVKDQFDEWYIAPLDVPRGMTLESLQNKLHEHSIENIQTFAAVR
                     EAYRAAASKAGENDRIVVFGSFHTVADVMSVL"
     gene            11786..12784
                     /gene="ftsN"
                     /locus_tag="WGQ68_001287"
     CDS             11786..12784
                     /gene="ftsN"
                     /locus_tag="WGQ68_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HEZ0715079.1"
                     /translation="MSENKQNEVLSGYEQLKRRNRRRLVTASCLVAASCILLAAALSS
                     GPAEQTAGETSGVENKAAGAAQTPALKSAADKPQDLAGEDKPSAADSEISEPENVGAP
                     LVLINDRLEDSNIKGLEASEKLQQAETAKTAPKQAKQRAAEKVPATADSTDTVAVEKP
                     KRTAETKPQKAERTAKAKPKAKETKTAEKVADKPKTAAEKTKPDTAKSDSAVKEAKKA
                     DKAESKKTAEKDRSDGKKHETAQKTDKADKTKTAEKEKSGKKAAIQAGYAEKERALSL
                     QRKMKAAGIDSTITEIMTDNGKVYRVKSSNYKNARDAERDLNKLRVHGIAGQVTNE"
     gene            12777..13274
                     /locus_tag="WGQ68_001288"
     CDS             12777..13274
                     /locus_tag="WGQ68_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEZ0715080.1"
                     /translation="MNNLPVADLLVFAIIAACIVLSAMRGVIAEAGSMAAWVVSFFFA
                     KLFAAPFADLAFASFQPRLFALALSFISLFILAYLIQKILRSLLTQAVSSVGLGFANR
                     ILGGVFGALKGVLIVTLLIMLASKTDLPDTEEWRQSYTLPFFVSLSEAVLNHADDSDG
                     ALEDD"
     gene            13586..15130
                     /gene="purF"
                     /locus_tag="WGQ68_001289"
     CDS             13586..15130
                     /gene="purF"
                     /locus_tag="WGQ68_001289"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEZ0715081.1"
                     /translation="MCGVLGLVSHAPVNQLLYDGLQMLQHRGQDAAGIATAEGGTFHM
                     HKGKGMVREVFRTRNMRDLTGNAGIAHVRYPTAGNAGSSAEAQPFYVSSPFGIVLAHN
                     GNLTNTAELYENVCNKHLRHINTGSDSEVLLNVFAHELRREVSKNADPHRLNADNIFN
                     AVAEVHRLVRGAYGVVAMIAGYGMVAFRDPYGIRPLALGSQTDESGRKSYAVASESVA
                     FNALAYDLERDIRPGEAVFVGFDGTMIARQCSDKAKLSPCLFEYVYFARPDSVIDGVS
                     VYQSRLDMGVSLAEKIKRELPVDDIDVVMPIPDTSRPSAMELAVYLKKPYREGLIKNR
                     YIGRTFIMPGQATRKKSVRQKLSPMETEFAGKSVLLVDDSIVRGTTSREIVEMVRAAG
                     ARKVYIASAAPEVRYPNVYGIDMPTREELIANGRSAAEIAAEIGADGIVFQDLGDLEA
                     VVKALNPKIESFDSSCFNGIYQTGDIDDAYLDRLSAEKSGCGGLKIHPSRMEHSISIS
                     DAGDEE"
     gene            15230..15721
                     /gene="greB"
                     /locus_tag="WGQ68_001290"
     CDS             15230..15721
                     /gene="greB"
                     /locus_tag="WGQ68_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEZ0715082.1"
                     /translation="MSTETKNYITPAGWQALKDELYRLVNKERPEIVQIVNWAAGNGD
                     RSENGDYLYGKRRMREIDRRIRFLTKRLESAVVVDPELREATDQVFFGATVGLLRGDG
                     REQTVKIVGIDEIDTAQNKISWISPLARCLIKAREGDEVVLNTPEGREEIEILSVEYI
                     KID"
     gene            15780..16406
                     /locus_tag="WGQ68_001291"
     CDS             15780..16406
                     /locus_tag="WGQ68_001291"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEZ0715083.1"
                     /translation="MRKIRTKICGITTLEDAAAAAAAGADAVGLVFFQGSSRAVDIAR
                     AKKITAALPPFVSVVALFVNESAQNIRRILAEVPIHIIQFHGDEDDAFCRQFDRPYIK
                     AIRVQTASDIRNAADRFPDAQALLFDAYHPSEYGGTGHRFDWTLLAEYSGKPWVLAGG
                     LTPENVGEAVRITGAEAVDVSGGVEASKGKKDPAKVAAFIATANRLSR"
BASE COUNT         4591 a         4447 c         3981 g         3413 t
ORIGIN      
        1 ccacgaacct acatcccgtt attaccacga aagcgggaat ctaggacgca gggttaagaa
       61 aacctacatc ccgtcattcc ctcaaaaaca gaaaaccaaa aacagaaacc taaaatcccg
      121 tcattcccgc acaagcggga atccaaactt gtccgcacgg aaacttatcg gataaaacgg
      181 tttcttagat tccacgttct agattcccgc ctgcgcggga atgacgaatc catccgtacg
      241 gaaacctgca ccgcgtcatt cccacgaacc tacatcccgt cattccctca aaaacagaaa
      301 accaaaatca gaaacctaaa atcccgtcat tcccaccact tttcgtcatt cccgcgaaag
      361 cgggaatcta gaatcccgga ctttcagata atctttgaat attgccgctg ccttaaggtc
      421 tagattcccg cctgcgcggg aatgacgaat ccatccgtac ggaaacctgc accgcgtcat
      481 tcccacgaac ccacatcccg ttattaccac aaaagcggga atctaggacg cagggttaag
      541 aaaacctaca tcccgtcatt ccctcaaaaa cagaaaacca aaaacagaaa cctaaaatcc
      601 cgtcattccc gcgcacgcgg aaatccaaaa acaaaaagcc gcagaaattt aacggaagaa
      661 ccgaaccccg aaaatcggct tcccgccttc gcttgaataa cagaacaaag gaagcggaaa
      721 taaaaacatc gggattcttt ttttaaattg gcaataaaac accaaaatga atgatttaca
      781 caacaaaaaa tagtcaacac cgccaaaccg gcgtaaaata caagacatta tcacccttaa
      841 aacggtaaaa ccttatgaaa accaaacgtt ttaaaattaa cgccatatcc ttatccatct
      901 ttcttgccta tgcccttacg ccatactcag aagcggcatt ggtcagagac gatgtcgatt
      961 atcaaatatt ccgtgacttt gcagaaaaca aaggcaaatt ttttgtcggc gcaaccgatt
     1021 tatcagtgaa aaacaaacaa ggtcaaaaca tcggcaacgc attatccaac gtaccgatga
     1081 ttgattttag cgttgcagat gtcaacaaac gcatagcaac ggtagtcgat ccccaatatg
     1141 ccgtcagcgt caaacacgcc aaagcagaag tccatacttt ttattacggt caatacaacg
     1201 gtcataatga tgtagccgac aaagaaaatg aataccgcgt tgtcgaacaa aataactatg
     1261 aaccccataa agcttggggt gcgagcaact taggtcgcct cgaagattat aatatggcgc
     1321 gttttaacaa gtttgtaact gaagttgcgc cgattgcacc aactgatgcc ggcggcggat
     1381 tggataccta caaagataaa aaccgttttt cctcttttgt cagagttggc gcaggcaggc
     1441 aattagttta tgaaaaaggg gcttatcatc aagaaggaaa tgaaaaaggc tacgatttgc
     1501 gcgatctttc acaagcctat cgttatgcca ttgcaggtac accttataaa gatattaata
     1561 ttgaccaaac aatgaatacc gaaggtttga ttggctttgg taatcataat aaacagtatt
     1621 cggcagaaga acttaaacaa gcactctcgc aagatgcgtt aacaaattac ggcgtattgg
     1681 gcgatagcgg ctctccgtta tttgcttttg acaaacaaaa aaatcaatgg gtctttttgg
     1741 gaacttatga ttattgggca ggttacggaa aaaaatcttg gcaagaatgg aatatctata
     1801 aaaaagaatt tgcagataaa atcaaacaac gcgataacgc cggcaccatc aaaggtaata
     1861 gagaacatca ttggaacatt acttctggca caaacagcaa gattggttcg acagcggtaa
     1921 ggcttgccaa caatgaaagg gatgcagaca acggacagaa tgttaccttt gaagacaacg
     1981 gtactttggt attggatcaa aacatcaacc aaggcgcggg cggtctgttt ttcaaaggcg
     2041 attacacagt caaaggtgca aataatgaca tcacttggct aggtgcgggg attgatgtta
     2101 ccgacggcaa aaaagtcgtt tggcaagtca aaaatccgaa tggcgacaga ttggcaaaaa
     2161 tcggcaaagg cacattagaa ataaacggca caggcgtaaa tcaaggcgaa ttaaaagtcg
     2221 gcgacggtac ggttattctg aatcaaaaag ccgattcaaa ccaaaaagtc tcggctttct
     2281 cccaagtcgg cattgtcagc ggacgcggta cattggtatt aaatagttca aatcagatta
     2341 atcccgataa cctatatttc ggtttccgtg gcggtcgttt ggatgccaat ggcaacgact
     2401 tgacttttga acacatccgc aacgtggatg aaggtgcacg cattgtcaac cacaacacag
     2461 gtcacgcctc cacaatcacg ctaacgggta aatctttaat taccaatccg aacagccttt
     2521 cggtacatag tatacaaaat gactatgatg aagatgatta tagttattat taccgaccaa
     2581 ggcgacccat cccacaaggc aaagatcttt attacaaaaa ctatcgttat tacgccctaa
     2641 aatccggcgg cagcgtgaac gcaccgatgc ccgagaacgg tgtaacagaa aataacgact
     2701 gggtctttat gggatatacg caagaagaag ccaagaaaaa cgcgatgaac cacaaaaaca
     2761 atcagcgtat tagcggtttt agcggtttct ttggcgaaga aaacggaaaa ggacataacg
     2821 gtgcattaaa ccttaatttc aacggcaaaa gcgcgcaaaa ccgtttcttg ttaacaggcg
     2881 gcactaattt aaacggaaaa ataagcgtaa ctcaaggcaa tgtcttgtta tcaggtcgtc
     2941 caacaccaca cgcaagagat tttgtgaaca aatcttcagc ccgaaaagac gcacgtttct
     3001 ccaaaaacaa tgaagtcgta tttgaagacg actggataaa ccgcacattc aaagccacag
     3061 aaattacggt taatcaatcc gcatcattct cttctggcag aaatgtatcc gacatcaccg
     3121 ctaacattac cgcaacagac aacgccaaag taaatttggg ttacaaaaac ggtgatgagg
     3181 tttgcgtgcg ctcggactat accggttacg ttacctgcaa cacagacaac ttatccgata
     3241 aagctttaaa cagctttgat gcgacacaga ttaacggcaa tgtgaatttg agtcagaatg
     3301 cggcattgac tttgggcaag gctgcattat gggggcaaat tcaagggcaa ggaaacagcc
     3361 gtgtcagcct aaaccaacat agcaaatggc atttgaccgg cgacagccaa gtacacaatc
     3421 tgtcattggc ggatagccat attcatttga acaacgcttc cgatgcgcaa agtgcaaata
     3481 aataccacac gctcaaaatc aatcatttat ccggtaacgg gcattttcat tatctgacgc
     3541 acttggcgga aaatcttggg gataaagtgc ttgtgaagga atccgcatcc ggtcattatc
     3601 agctccatgt tcaagataaa acaggcgaac ccaatcagga agggctgaat ctctttgatg
     3661 catcatccgt acgagaccgt tctcaccttt ccgtttcctt ggcgaatcat cacgtcgatt
     3721 taggcgcatt gcgttataca atcaaaacag aaaacggtat tacccgattg tacaatcctt
     3781 atgccgagaa ccgccgccga gtcaaaccgg ccccgtctcc tgccacaaac acggcttctc
     3841 aagcacaaaa ggcaacacaa acggacggtg cacaaattgc caagcctcaa aatatcgtcg
     3901 tcgcaccgcc tagcccgcag gcaaatcaag ccgaagaagc aaaacgccaa caagcaaaag
     3961 cggagcaagt gaagcgtcag caagcagagg cagaaaaagt cgcacatcaa aaggctgaag
     4021 aagcgaaacg tcagcaagat gcccttgccc gccaacaagc agagcaagaa cgccaacgat
     4081 tggaagccga acgccaagct gccgaaattg ccaaacaaaa agcagaagcc gaagaagcga
     4141 aacgccgagc cgctgaaatt gccgagcaaa aagcagctgc cgaagaagca aaacgccaag
     4201 ctgccgaact tgcacgccaa caagaagaag cacgaaaagc tgcagagttg gctgctaagc
     4261 aaaaagcgga aactgaacgc aaagcagcag aaattgccga gcaaaaagcg gaagccgaac
     4321 gtgaagccgc cgaacttgcc aagcaaaagg cggaagaaga aggtcgccaa gcagcacaat
     4381 ctcaacccaa acgtcgtaac cgcagggcta taccaccgga actgtcatct gatgcaacaa
     4441 cccgagcttt gcctaggata gcgagaaatt caaatcctga tgccagcgat tatgaagaaa
     4501 tccccttgga tgcgttggaa gatgaagatg tatccgaatc ggttgacaca tcagacaaac
     4561 agcctcaaga caatacggaa cttcatgaaa aagttgagac agtgagtttg caaccaagag
     4621 ccgctcagcc gcgagcccaa gccgccgcgc agccgcaagc ccaagccgtc gcgcaagccg
     4681 atgcagtcag caccaatacc aactcggctt tatctgacgc aatggcaagc acgcaatcta
     4741 tcttgttgga tacaggtgct tcattaacac ggcacattgc acaaaaatca cgtgctgatg
     4801 ccgaaaaaaa cagcgtttgg atgtcgaaca tcggttatgg ccgtgattat gcttccgcac
     4861 aatatcgccg gtttagttcg aaacgcacgc aaacacaaat cggcattgac cgcagcttgt
     4921 ccgaaaatat gcagataggc ggcgtattga cttactctga cagtcagcat acttttgatc
     4981 aggcgagcgg caaaaatact tttgtgcaag ccaaccttta tggtaagtat tatttaaatg
     5041 atgcttggta tgtggccggc gatattggtg caggcagctt gagaagccgg ttacaaacgc
     5101 agcaaaaagc aaactttaac cgagcaagca tccaaaccgg ccttactttg ggcaatacgc
     5161 tgaaaatcaa tcaattcgag attgtcccca gtgcggggat ccgttacagc cgcctgtcat
     5221 ctgcagatta caagttgggt aacgacagtg ttaaagtaag ttctatgtca gtgaaaacac
     5281 tgacggccgg actggatttt gcttatcggt ttaaagtcgg caaccttacc gtaaaaccct
     5341 tgttatctgc agcttacttt gccaattatg gcaaaggtgg cgtgaatgtg ggcggtaatt
     5401 ccttcgccta taaagcagat aatcaacagc aatattcagc aggtgccgcg ttactgtacc
     5461 gtaatgttac attaaacgta aatggcagta ttacaaaagg caaacaattg gaaaaacaaa
     5521 aatccggaca aattaaaata cagattcgtt tctaaaatac taaattcata gcaaaataaa
     5581 atgccgtctg aactcaggct tcagacggca tttttatagt ggattaacaa aaatcaggac
     5641 aaggcgacga agccgcagac agtacagata gacggcaagg cgaggcaacg ccgtactggt
     5701 ttttgttaat ccactatatc agacggacga ggttacagtt taatcccttt gagcttcgcg
     5761 acggtattga tgtctttgtc gccacgacct gagaggttga ccaaaatcac ttggtcttta
     5821 cccattttcg gcgcattttt caccgcccaa gcaacggcgt ggctggattc gagcgcgggg
     5881 atgatgccct cgaagcggca gagcaagtca aaggcttcga gtgcttcgtc gtctttggca
     5941 acggtgtatt cgacgcgctt gatgtcgtgc agatggctgt gttccgggcc gatgccgggg
     6001 taatccaagc ctgcggaaac agagtgcgta cccaaaacct gaccgttttc gtcctgcatc
     6061 agatagctgc ggaaaccgtg caatacgcca atcggtgcgc ccgaagtaat cggcgcggcg
     6121 tgatcggggg tgttcacgcc caaaccgcca gcctccacgc cgacgaggcg cacgttttct
     6181 tcgccgatat acgggtggaa caaaccgatg gcgttcgatc cgccgcccac gcaggcaacg
     6241 gcaacgtcgg gctgtctgcc gatggcttcc tgcatctgcg ctttagcttc gttgccaatc
     6301 acgcattgga aatcgcgcac catttcggga tacggcgcgg ggccggcggc ggtaccgatg
     6361 atgtagaacg tgtcgtccac gcgggcgacc cattcgcgca tggcttcgtt catcgcgtct
     6421 ttcagcgtgc ggctgccgct ttctacaccg accacgttcg cacccaataa tttcatacgg
     6481 aacacgttgg gcatttggcg ttggatgtcg tccgcgccca tatacacgtc gcaagtcata
     6541 ccgaagcgtg cggcaacggt ggcactcgcc acgccgtgct gacccgcgcc ggtttcggcg
     6601 atgacgcgtt ttttacccat gcgttttgcc aacagtgcct gaccgatggt gttgttgact
     6661 ttgtgcgcgc cggtgtggtt caagtcttcg cgcttcaacc agatttgcgc gccgcccaga
     6721 tgttcggaca accgcgcggc gtggtaaacg gggctgggcc tgccgacata atgtttcaaa
     6781 tcatggcgga acgcttccca aaattcagga tcgtttttcg ctgccttata ggcatccgcc
     6841 agctcttgca aggcgggaat cagggtttcg gagacataaa gcccgccgtg ttcgccgaaa
     6901 aagcccttct cgtcgggcgc gtggtagttc ttcatcggat tcttccttgt gcttcaaatt
     6961 ttcagacggc atttccgttt ttgcaaacat gccgtctgaa cgggaaattg tcggcaatta
     7021 taactgtttt tcacacaagc ccgccatttc aaaaaacgcc cgatacgccg ccgctttttt
     7081 cgccaaactc aaaggatagg cgcgcgaagt cgaaggcagg cgcgtcagcg tcaaatcccg
     7141 tccggcaaac ggaaacggca cggtttcgcc cgttttcggc attttgatac cgccgccctg
     7201 aatatcgagc aggatttccg tcgccttgcc gcctgtcgta caaatatggc ggcactcggg
     7261 tatttttgcc aacaccgccg ccaaatcgac ggtttcaact acctttaaaa acttgtcgga
     7321 cgcattgccg tgctgacgta ccgccttcaa aacggtcgga caggacgcaa tcccccgttc
     7381 gtgcaaaaac gccttgattt tctcggcatc aaacgctttt tcagacaacc tttggaaatg
     7441 cgccgcatca ttaaaaaaca ccagcccata aacgcgccac atatcgtttt ggaaattcgg
     7501 ataatgaaac tgcatcgcgc gtttgtcttc cttgggcgga aacgtcccca tcatcatgac
     7561 agcagccttc ggcggcaaca cgggatcgaa gggatgggtt tcgatttcaa gcagattttc
     7621 agacacgttc aatcctccaa caggcaaaac ggcagacagc cggtttttgc accaaaccgc
     7681 caaacacggc aagccttcaa acagcattat cacgccctgc cgttttcggt tacaatgccg
     7741 acatcaaacg gatactgtaa acacatttat gtttcaacac acaggacgac acataaagca
     7801 ccgccctatg tgccatcctg atttggaaag agttacgccc ctcccaaata gagtctgatt
     7861 ctaccgccct aaagggcggg gtttcaaccg aaaaggaaac acgatgaaag aacacaaagc
     7921 ccgcaagcgt ttcgggcaga attttttgca ggacacgcgg attatcagcg atattgtcaa
     7981 cgccgtgcgc ccgcaggcgg atgatgtcgt gattgaaatc ggcccgggtt tggcggcgat
     8041 taccgaacct ttggcgaaaa agctgaaccg cctgcacgtt gtcgaaatcg accgcgacat
     8101 cgtatgccgt ctgaaaacgc tgccgtttgc ggataaactg gtgattcacg aaggcgatgt
     8161 attgcagttt gatttcaacg gcattgaagg caaaaagaaa atcgtcggca acctgccgta
     8221 caatatttcc acgccgcttt tgttcaagct ggcggaggtg gcggacgatg tcgtcgatat
     8281 gcactttatg ctgcaaaaag aagtggtcga gcgtatggtt gccgcgccga aaagcaacga
     8341 ctacggccgc ttgggcgtga tgctgcaata ttttttcgat atggagctgc tgattgacgt
     8401 accgcccgaa tcgttcgatc ctgcaccgaa agtggattcc gccgtcgtcc gtatgattcc
     8461 ggtgaaacac cgcatcggca aggcggacga tttcgagcat ttcgccaaac tcgtaaaact
     8521 cgccttccac caacgccgca aaaccatacg caacaacctg aaagaactgg caacagacga
     8581 agatttgcag gcagccggca tcaatccgca agaccgtgcc gaacatatcg cgccggagaa
     8641 atatgtggcg ttgagcaatt atctggcggg caaggccgtc tgaaaaggga gtaagccgat
     8701 gacgaaatac ctgcttttac ccctgatact gccgcttgca gcctgcctga ccaatccgaa
     8761 cgaccgtttt tttgacggca gacgctacac gcttcatcat atggagctcc cgtaacgaca
     8821 acctgcaacg gcaaatgccg tctgaaagaa aagacatgat caaattcaaa aacgtacaca
     8881 aacatttcaa agacctgcac gtcatcaacg gcgtgaactt ggaaatcaaa aaaggcgaag
     8941 tggtcgtcgt ctgcggacct tcgggcagcg gcaaatccac actcatccgc accgtcaacc
     9001 agcttgaaag catcgaaagc ggcgagattt gggtggacgg tgtcaacgtt gccgacccaa
     9061 aaaccgattt gaacaaaatc cgcgaagaag tcggctttgt gtttcaaagt ttcaacctct
     9121 acccccacct gaccgtattg gaaaacatca ccctcgcacc gatgaaggtt aaaggtcaaa
     9181 atgcggaaca ggcggagaaa aaagcgatgg aacttttgga acgcgtcgga ctggcgcaca
     9241 aaaaagacgc ttttccgtcc caactttccg gcggccagca gcagcgcgtg gcgattgcac
     9301 gcggtttggc gatggaaccg cgcgtaatgt tgtttgacga accgacctcc gcactcgacc
     9361 ccgaaatggt cggcgaagtg ttgaaagtga tgaaagactt ggcagaaagc ggcatgacca
     9421 tgatgtgcgt aacccacgaa atggggtttg cccgcgaagt tgccgaccgc gtgattttcg
     9481 tcgataaagg gcaaatcctc gaagacgaaa cgcccgaagc gtttttcacc aacccgaaac
     9541 acgaacgcgc caagcagttc ctgcaacagg tcatgaccca ctgatgctat accgtctgaa
     9601 aaccgaacgt tcagacggca tagcccgaca ccgcaagatg attgagaaag aaaaccatgt
     9661 attttgttga ccgcaccgcc gtcgtcctca agccgaccgt ccgctttttg gaatggctca
     9721 aaagcaccga cgaaaatatg cccgacctga ccatagagca gcttcgtgcc aactgctccg
     9781 tcttcctcgt tccgcagttc gacgaaccgg aagccgtcgt ttcctatttc gacgaacgct
     9841 accgccagat ttttgaagcc gaactcgccg gctgggacat cgacaaggat aaatggccgc
     9901 aagatatggg gctgaaggcg ttttgggaat ttttcgacat cgaaatccac gatatggtgt
     9961 tggatatgga agaagcggat ttgaacatca cgcccgtgtt cgacaatatg atgtaacgcc
    10021 gtgcgcgcct ttcagacagc attgaggccg tcccgcatcc tgaacatcct gaccgtatcg
    10081 ctccactcgg cttcccttgc cgtctgcctg acctggtttt acggccggat gatgtggttc
    10141 ggtttggcgg ctttggcggc aagttacgca tactcgctca ggataacgaa tctgaaacac
    10201 cgccatgcca taaccgccat taccatagac cgcgacggac aggcggaaat cgtatccggc
    10261 aaagaaaaaa cagcaaaggc ggcggcactg tcgggcagca gtatggttac gccttatgcc
    10321 ttattcttac aatgggacac cggcggcaaa accgtccgcc actgcatcac ccacgatatg
    10381 gcggacccgg aatcctaccg gcggctgaaa gtctgggtgc tttggcggca gccgaaaaaa
    10441 accgccgaaa ccgatacacc aagctgattt cagacggcat ttgaacacgg aacactcaat
    10501 gaaaacactg caagactggc tctcccattt ggaaaccgcc cacagcggcg gtttgatcga
    10561 tatgggcttg gaacgcgtag gcgaagtgaa aaaacggatg aagctcacgc cgcaatgccc
    10621 cgtcgtcgtt gtcgcgggaa ctaacggcaa aggttcggtc tgcgcctacc tgacacagat
    10681 ttacaaacag gcgggctaca aaaccggcac attaaccagc ccccatttat tgcgttacaa
    10741 cgaacgcatc gccatcaacg ccgaacccgt ttcggacgat acgattattg cctctttcga
    10801 gcgcatcgaa gcggcgcgcg gcgaaatatc gctgacttat tttgaattca atacgttggc
    10861 ggccgtcgac atcttcatgc gcgaacaggt tgacgtgatg atattggaag tcggcttggg
    10921 cggacgcttg gacgcggtca acgcatttga cggcgattgc gcggtggtta ctagtgtaga
    10981 ttttgaccat caggcgtttt tgggcgatac ggtcgagcag gtcggctttg aaaaagcagg
    11041 cgtgttccgc ggcggcaaac ccgcaatctg cggtcaaaat cccgcgcccg catcgttgct
    11101 ttcacacgcc gaagccatag gcgcgaaact gctgatggtg cagcgcgatt tcgagtttca
    11161 cgcgctggaa aacatccaat ggaactaccg cttccgaccg cagcattcag acagccccgc
    11221 tcgcaaccgt aattccctgc ccttccccgc attgcgcggc gcataccaac tttccaacgc
    11281 cgcctgcgcg ctgaccgtgt tggaatgttt gaacgacaaa ttgccggtgg acatcggtgc
    11341 aatcaaacgc ggattgctgc tggttgaaaa tcccggacgc ttccaagtcc tgcccggccg
    11401 gccgctgacc gttttggacg tcggacacaa tccccacgct gcccgcgccc tgcgccgcag
    11461 cctgattaat ttggcgtttg cgcaaaaacg caccgccgta ttcagtatgc tgtccgacaa
    11521 agacatagac ggcgtgttgg aaatcgttaa agaccagttt gacgagtggt atatcgcacc
    11581 gttggacgta ccgcgcggca tgacgctcga gagcctgcaa aacaaactgc acgaacacag
    11641 catcgaaaac atacagacat tcgccgccgt ccgcgaagca taccgggcgg cagcctcaaa
    11701 agcgggcgag aacgacagaa tcgtcgtttt cggctcattt cataccgttg ccgatgtcat
    11761 gtcggtactt taaaaggaaa cttctatgtc cgaaaacaaa caaaacgaag tcctgagcgg
    11821 ttacgaacaa ctcaaacggc gcaaccgccg ccgcctcgta acggcaagtt gcctggttgc
    11881 cgcctcctgc atcctgctgg cagccgccct cagttccggc cctgccgaac agactgccgg
    11941 cgaaacaagc ggcgtagaaa acaaagcggc aggtgcggca caaacccctg ccttgaaatc
    12001 cgccgccgac aaaccgcagg acttggcagg cgaagacaag ccttctgccg ccgacagcga
    12061 aatcagcgag cctgaaaacg taggcgcgcc gctggtgctg attaacgacc gcctcgaaga
    12121 cagcaacatc aaaggtttgg aagcatccga gaaactgcaa caggcagaaa ccgccaaaac
    12181 cgcaccgaag caggcaaaac aacgcgctgc cgaaaaagtg ccggcaactg ccgacagtac
    12241 ggatacggta gcggttgaaa aaccgaaacg cactgccgaa acaaaaccgc aaaaagcgga
    12301 acgcactgcc aaagccaagc ccaaagccaa agaaaccaaa accgccgaaa aagttgccga
    12361 caaaccgaaa actgccgccg aaaaaaccaa accggatacg gcaaaatccg acagcgcggt
    12421 aaaagaagcg aaaaaagccg acaaggctga aagcaaaaaa acagccgaaa aagaccgttc
    12481 ggacggcaaa aaacacgaaa cggcacaaaa aaccgacaaa gcggacaaga ccaaaaccgc
    12541 cgagaaggaa aaatccggta aaaaagccgc cattcaggca ggttatgccg aaaaagaacg
    12601 cgccttaagc ctccagcgca aaatgaaggc ggcgggtatc gattcgacca tcaccgaaat
    12661 tatgaccgac aacggcaaag tttaccgcgt caaatcaagc aactataaaa acgcaaggga
    12721 tgccgaacgc gatttgaaca aattgcgcgt acacggtatc gccggtcagg taacgaatga
    12781 ataacctccc cgttgccgac ctcctcgtct tcgccatcat cgccgcctgc atcgtgctat
    12841 ccgcgatgcg cggcgtgatt gcggaggcag gttcgatggc ggcatgggtg gtttccttct
    12901 tttttgccaa actctttgcc gcacccttcg ccgacctcgc ctttgcctcg ttccagccgc
    12961 gcctgttcgc actggcattg tcgtttattt cattattcat cctcgcctat ctgatacaga
    13021 aaatactccg ctcgctgctg acacaagcag tttcctccgt cggcttgggc tttgcaaacc
    13081 gcattttggg cggcgtattc ggtgcattga aaggcgtttt gattgttacc ctgctgatca
    13141 tgcttgcttc aaaaaccgac ctgcccgata ccgaagaatg gcggcaatct tacacactgc
    13201 cgtttttcgt atcgctttcc gaagcagtgt taaaccatgc ggacgacagt gatggagctt
    13261 tggaagacga ttgattgcca tacggatatt tcagacggca tcctctttta tgccgtctga
    13321 aatgtttttt cctttcctca gccatatcac agattatagt ggattaaatt taaaccagta
    13381 cggcgttgcc tcgccttgcc gtactatttg tactgtctgc ggcttcgtcg ccttgtcctg
    13441 atttttgtta atccactata aatcttattc tccataaacc cacctatctg tccttcactc
    13501 aaatattgtc aacaattcag gcaaaaaacc gccgtttgct ttataattcc gttttaccct
    13561 taaacctcga atcggagaag acgatatgtg cggcgtatta ggtttggtca gtcatgcacc
    13621 cgtgaaccag cttctgtacg acggcttgca gatgttgcag cacagggggc aggatgcggc
    13681 gggcattgca acggcggaag gcggcacgtt ccatatgcac aaaggcaaag gcatggtgcg
    13741 cgaagtgttc cgtacacgca atatgcgcga tttgaccggc aacgccggca tcgcccacgt
    13801 ccgttatccc acggcgggca acgccggcag cagcgcggag gcgcagcctt tctacgtcag
    13861 ctcgccgttc ggcatcgttt tggcacacaa cggcaacctc accaacacgg ccgaactgta
    13921 tgaaaacgtg tgcaacaaac acctgcgcca catcaacacc ggctccgatt ccgaagtctt
    13981 gctcaacgta ttcgcgcacg aattgcgccg cgaagtttct aaaaacgccg acccgcaccg
    14041 gctcaatgcc gacaatattt tcaacgccgt tgccgaagtc caccgcctgg tgcgcggcgc
    14101 atacggcgtg gttgccatga ttgcgggcta cggcatggtc gccttccgcg atccttatgg
    14161 catccgtccg ctggcattgg gttcgcaaac cgacgaatca ggcagaaaat cctatgccgt
    14221 cgcctccgaa tccgttgcct tcaatgcgct tgcctacgat ttggaacgcg atatccggcc
    14281 gggcgaagcg gtatttgtcg gctttgacgg cacaatgatt gcccgccaat gcagcgataa
    14341 ggccaaactc agcccctgcc tttttgaata tgtttatttt gcccgccccg actctgtgat
    14401 tgacggcgta tccgtttacc aatcgcgctt ggatatgggc gtgtccttgg cggaaaaaat
    14461 caaacgcgag ctgcccgtgg acgacatcga cgtcgtgatg cccattcccg acaccagccg
    14521 ccccagcgcg atggagcttg ccgtctacct caaaaagcct tatcgcgaag gcttgattaa
    14581 aaaccgctat atcggccgca cctttattat gcccggtcag gcgacgcgca aaaaatccgt
    14641 gcgccaaaaa ctcagcccga tggaaaccga atttgcaggc aaaagcgtgt tgctggtgga
    14701 cgactccatc gtgcgcggga cgaccagccg cgaaatcgtc gaaatggtac gcgcggctgg
    14761 cgcgcgcaaa gtctatatcg cctccgccgc gcccgaagtg cgctatccca acgtatacgg
    14821 catcgatatg cccacacgcg aagagttgat tgccaatggg cgcagcgcag cggaaattgc
    14881 cgccgaaatc ggcgcggacg gcatcgtgtt tcaagacttg ggcgatttgg aagccgtcgt
    14941 caaagcactc aatccgaaaa tcgaatcttt cgattcgtcc tgtttcaacg gcatttatca
    15001 aaccggcgac atcgatgatg cctacctcga ccgcctgtct gccgagaaat ccggctgcgg
    15061 cggcctgaaa atccacccga gcaggatgga acacagcatc agcatcagcg atgcgggcga
    15121 cgaagaataa ggcttcccaa accgcgatgc cgtctgaaat ccgaacgccg ggttttcaga
    15181 cggcatttcc catatcggca tcacatttcc catttcatcc gcacgcaata tgagtaccga
    15241 aaccaaaaac tacattacgc ccgccggatg gcaggcattg aaagacgagc tttaccggct
    15301 ggtcaacaaa gaacgccccg aaatcgtcca aatcgtcaac tgggcggcag gcaacggcga
    15361 ccgcagcgaa aacggcgact atctttacgg caaacgccgt atgcgcgaaa tcgaccgccg
    15421 catccgtttc ctgaccaaac gtttggagtc tgccgtcgtc gtcgatcccg aattgcgcga
    15481 agcgaccgac caagtgtttt tcggcgcgac ggtcgggctg ttgcgcggcg acgggcgcga
    15541 gcaaaccgtc aaaattgtcg gcatcgatga aatcgatacc gcgcaaaaca aaatttcttg
    15601 gatttcgccg ttggcgcgtt gcctgattaa agcgcgcgaa ggcgacgaag tggttttgaa
    15661 cacgccggaa gggcgtgagg aaattgaaat cctttctgtc gaatacatca aaatcgactg
    15721 atcgggaatg ccgtctgaaa cggcacaaac gcatcgtttc ccaaataaag gaataccgaa
    15781 tgaggaaaat ccgcaccaaa atctgcggca tcaccacact ggaagacgca gcggccgccg
    15841 cagcggcagg tgcggatgcc gtcgggctgg tctttttcca aggcagcagc cgggccgtcg
    15901 atattgcccg cgccaaaaaa atcaccgccg cactgccgcc gtttgtcagc gttgtcgccc
    15961 ttttcgtcaa cgaaagcgcg caaaacatcc gccgcatcct tgccgaagtg ccgatacaca
    16021 tcatccaatt ccacggcgac gaagacgacg cattctgccg ccagttcgac cgcccctata
    16081 tcaaggccat tcgtgttcag acggcatcag acatccgaaa cgccgccgac cgcttccccg
    16141 acgctcaggc actgctgttc gatgcctacc acccttcgga atacggcggc accggacacc
    16201 gcttcgactg gacgctgctg gcggaatatt cgggcaaacc gtgggtgctt gccggcgggc
    16261 tgacccctga aaacgtcggc gaagccgtcc gcatcaccgg agcggaagcg gtcgacgtat
    16321 ccggcggcgt ggaagcgtct aaaggcaaaa aagacccagc caaagtcgcc gcctttatcg
    16381 caaccgccaa ccgcctatcc cgttaaagca acaaaaattg ccgctggaat ga
//
LOCUS       DAWFHY010000047        16140 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.047, whole genome shotgun sequence.
ACCESSION   DAWFHY010000047 DAWFHY010000000
VERSION     DAWFHY010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16140
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.047"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WGQ68_001292"
     CDS             complement(86..2191)
                     /gene="fusA"
                     /locus_tag="WGQ68_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEZ0715084.1"
                     /translation="MARKTPISLYRNIGISAHIDAGKTTTTERILFYTGLTHKLGEVH
                     DGAATTDYMEQEQERGITITSAAVTSYWSGMAKQFPEHRFNIIDTPGHVDFTVEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYQVPRLAFVNKMDRQGANFFRVVEQMKT
                     RLRANPVPIVIPVGAEDNFSGVVDLLKMKSIIWNEADKGTTFTYGDIPAELVETAEEW
                     RQNMIEAAAEASEELMDKYLGGDELTEEEIVGALRQRTLAGEIQPMLCGSAFKNKGVQ
                     RMLDAVVELLPAPTDIPPVQGVNPNTEEADSRQASDEEKFSALAFKMLNDKYVGQLTF
                     IRVYSGVVKSGDTVLNSVKGTRERIGRLVQMTAADRTEIEEVRAGDIAAAIGLKDVTT
                     GETLCAESAPIILERMEFPEPVIHIAVEPKTKADQEKMGIALNRLAKEDPSFRVRTDE
                     ESGQTIISGMGELHLEIIVDRMKREFGVEANIGAPQVAYRETIRKAVKAEYKHAKQSG
                     GKGQYGHVVIEMEPMEPGGEGYEFIDEIKGGVIPREFIPSVDKGIRDTLPNGIVAGYP
                     VVDVRIRLVFGSYHDVDSSQLAFELAASQAFKEGMRQASPALLEPIMAVEVETPEEYM
                     GDVMGDLNRRRGVVLGMDDDGIGGKKVRAEVPLAEMFGYSTDLRSATQGRATYSMEFK
                     KYSEAPAHIAAAVTEARKG"
     gene            complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WGQ68_001293"
     CDS             complement(2210..2680)
                     /gene="rpsG"
                     /locus_tag="WGQ68_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEZ0715085.1"
                     /translation="MPRRREVPKRDVLPDPKFGSVELTKFMNVLMIDGKKSVAERIVY
                     GALEQIEKKTGKVAIEVFNEAIANAKPIVEVKSRRVGGANYQVPVEVRPSRRLALAMR
                     WVRDAARKRGEKSMDLRLAGELIDASEGRGGALKKREEVHRMAEANKAFSHFRF"
     gene            complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WGQ68_001294"
     CDS             complement(2798..3169)
                     /gene="rpsL"
                     /locus_tag="WGQ68_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEZ0715086.1"
                     /translation="MPTINQLVRKGRQKPVYVNKVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCKVRLTNGFEVISYIGGEGHNLQEHSVVLIRGGRVKDLPGVRYHTVRGSL
                     DTAGVKDRKQARSKYGAKRPK"
     gene            complement(3427..7602)
                     /gene="rpoC"
                     /locus_tag="WGQ68_001295"
     CDS             complement(3427..7602)
                     /gene="rpoC"
                     /locus_tag="WGQ68_001295"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEZ0715087.1"
                     /translation="MNLLNLFNPLQTAGMEEEFDAIKIGIASPETIRSWSYGEVKKPE
                     TINYRTFKPERDGLFCAKIFGPVKDYECLCGKYKRLKFKGVTCEKCGVEVTLSKVRRE
                     RMGHIELAAPVAHIWFLKSLPSRLGMVLDMTLRDIERVLYFEAFVVTDPGMTPLQRRQ
                     LLTEDDYYNKLDEYGDDFDAKMGAEGIRELLRTLNVAGEIEILRQELESTGSDTKIKK
                     IAKRLKVLEAFHRSGMKLEWMIMDVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVIN
                     RNNRLKRLLELHAPDIIVRNEKRMLQEAVDSLLDNGRRGKAMTGANKRPLKSLADMIK
                     GKGGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIFHKLEKQGL
                     ASTVKAAKKLVEQEVPEVWDILEEVIREHPIMLNRAPTLHRLGIQAFEPILIEGKAIQ
                     LHPLVCAAFNADFDGDQMAVHVPLSLEAQMEARTLMLASNNVLSPANGEPIIVPSQDI
                     VLGLYYMTRDRINAKGEGSLFADVKEVHRAYHTKQVELGTKITVRLREWVKNEAGEFE
                     PVVNRYETTVGRALLSEILPKGLPFEYVNKALKKKEISKLINASFRLCGLRDTVIFAD
                     HLMYTGFGFAAKGGISIAVDDMEIPKEKAALLAEANAEVKEIEDQYRQGLVTNGERYN
                     KVVDIWGRAGDKIAKAMMDNLSKQKVIDRDGNEVDQESFNSIYMMADSGARGSAAQIK
                     QLSGMRGLMAKPDGSIIETPITSNFREGLTVLQYFIATHGARKGLADTALKTANSGYL
                     TRRLVDVTQDLVVVEDDCGTSDGFVMKAVVQGGDVIEALRDRILGRVTASDVVDPSSG
                     ETLVEAGTLLTEKLVDMIDQSGVDEVKVRTPITCKTRHGLCAHCYGRDLARGKLVNAG
                     EAVGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAASQVEAKSNGTARFSSQMRYVANN
                     KGELVVIGRSCEVVIHDDIGRERERHKVPYGAILLVQDGMAIKAGQTLATWDPHTRPM
                     ITEHAGMVKFENVEEGVTVAKQTDDVTGLSTLVVIDGKRRSSSASKLLRPTVKLLDEN
                     GVEICIPGTSTPVSMAFPVGAVITVREGQEIGKGDVLARIPQASSKTRDITGGLPRVA
                     ELFEARVPKDAGMLAEITGTVSFGKETKGKQRLIVTDVDGVAYETLISKEKQILVHDG
                     QVVNRGETIVDGAVDPHDILRLQGIEALARYIVQEVQEVYRLQGVKISDKHIEVIIRQ
                     MLRRVNIADAGETGFITGEQVERGDVMLANEKALEEGKEPARYENVLLGITKASLSTD
                     SFISAASFQETTRVLTEAAIMGKQDELRGLKENVIVGRLIPAGTGLTYHRSRHQQWQE
                     VEQETAETQVTDE"
     gene            complement(7759..11937)
                     /gene="rpoB"
                     /locus_tag="WGQ68_001296"
     CDS             complement(7759..11937)
                     /gene="rpoB"
                     /locus_tag="WGQ68_001296"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEZ0715088.1"
                     /translation="MNYSFTEKKRIRKSFAKRENVLEVPFLLATQIDSYAKFLQLENA
                     FDKRTDDGLQAAFNSIFPIVSHNGYARLEFVHYTLGEPLFDIPECQLRGITYAAPLRA
                     RIRLVILDKEASKPTVKEVRENEVYMGEIPLMTPSGSFVINGTERVIVSQLHRSPGVF
                     FEHDKGKTHSSGKLLFSARIIPYRGSWLDFEFDPKDLLYFRIDRRRKMPVTILLKALG
                     YNNEQILDIFYDKETFYLSSNGVQTDLVAGRLKGETAKVDILDKEGNVLVAKGKRITA
                     KNIRDITNAGLTRLDVEPESLLGKALAADLIDSETGEVLASANDEITEELLAKFDING
                     VKEITTLYINELDQGAYISNTLRTDETAGRQAARVAIYRMMRPGEPPTEEAVEQLFNR
                     LFFSEDSYDLSRVGRMKFNTRTYEQKLSEAQQNSWYGRLLNETFAGAADKGGYVLSVE
                     DIVASIATLVELRNGHGEVDDIDHLGNRRVRSVGELTENQFRSGLARVERAVKERLNQ
                     AESENLMPHDLINAKPVSAAIKEFFGSSQLSQFMDQTNPLSEVTHKRRVSALGPGGLT
                     RERAGFEVRDVHPTHYGRVCPIETPEGPNIGLINSLSVYARTNDYGFLETPYRRVIDG
                     KVTEEIDYLSAIEEGRYVIAQANADLDSDGNLIGDLVTCREKGETIMATPDRVQYMDV
                     ATGQVVSVAASLIPFLEHDDANRALMGANMQRQAVPCLRPEKPMVGTGIERSVAVDSA
                     TAIVARRGGVVEYVDANRVVIRVHDDEATAGEVGVDIYNLVKFTRSNQSTNINQRPAV
                     KAGDVLQRGDLVADGASTDLGELALGQNMTIAFMPWNGYNYEDSILISEKVAADDRYT
                     SIHIEELNVVARDTKLGAEDITRDIPNLSERMQNRLDESGIVYIGAEVEAGDVLVGKV
                     TPKGETQLTPEEKLLRAIFGEKASDVKDTSLRMPTGMSGTVIDVQVFTREGIQRDKRA
                     QSIIDSELKRYRLDLNDQLRIFDNDAFDRIERMIVGQKANGGPMKLAKGSEITTEYLA
                     GLPSRHDWFDIRLTDEDLAKQLELIKVSLQQKREEADELYEIKKKKLTQGDELQPGVQ
                     KMVKVFIAIKRRLQAGDKMAGRHGNKGVVSRILPVEDMPYMADGRPVDIVLNPLGVPS
                     RMNIGQILEVHLGWAAKGIGERIDRMLKEQRKAGELREFLNKLYNGSGKKEDLDALTD
                     EEIIELASNLRKGASFASPVFDGAKESEIREMLNLAYPSDDPEVEKLGFNDSKTQITL
                     YDGRSGEAFDRKVTVGVMHYLKLHHLVDEKMHARSTGPYSLVTQQPLGGKAQFGGQRF
                     GEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYENIVKGEHKIDAGMPESFNVLVK
                     EIRSLGLDIDLERY"
     gene            complement(12127..12498)
                     /gene="rplL"
                     /locus_tag="WGQ68_001297"
     CDS             complement(12127..12498)
                     /gene="rplL"
                     /locus_tag="WGQ68_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEZ0715089.1"
                     /translation="MAITKEDILEAVGSLTVMELNDLVKAFEEKFGVSAAAVAVAGPA
                     GAGAADAEEKTEFDVVLASAGDQKVGVIKVVRAITGLGLKEAKDIVDGAPKTIKEGVS
                     KAEAEDIQKQLEEAGAKVEIK"
     gene            complement(12556..13056)
                     /gene="rplJ"
                     /locus_tag="WGQ68_001298"
     CDS             complement(12556..13056)
                     /gene="rplJ"
                     /locus_tag="WGQ68_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEZ0715090.1"
                     /translation="MSLNIETKKVAVEEISAAIANAQTLVVAEYRGISVSSMTELRAN
                     ARKEGVYLRVLKNTLARRAVQGTSFAELADQMVGPLVYAASEDAVAAAKVLHQFAKKD
                     DKIVVKAGSYNGEVMNAAQVAELASIPSREELLSKLLFVMQAPVSGFARGLAALAEKK
                     AGEEAA"
     gene            complement(13286..13981)
                     /gene="rplA"
                     /locus_tag="WGQ68_001299"
     CDS             complement(13286..13981)
                     /gene="rplA"
                     /locus_tag="WGQ68_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEZ0715091.1"
                     /translation="MAKVSKRLKALRSSVEANKLYAIDEAIALVKKAATAKFDESVDV
                     SFNLGVDPRKSDQVIRGSVVLPKGTGKTTRVAVFTQGANAEAAKEAGADIVGFEDLAA
                     EIKAGNLNFDVVIASPDAMRIVGQLGTILGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VQYRTDKAGIVHATIGRASFAEADLKENFDALLDAIVKAKPAAAKGQYLKKVAVSSTM
                     GLGIRVDTSSVNN"
     gene            complement(13981..14415)
                     /gene="rplK"
                     /locus_tag="WGQ68_001300"
     CDS             complement(13981..14415)
                     /gene="rplK"
                     /locus_tag="WGQ68_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEZ0715092.1"
                     /translation="MAKKIIGYIKLQIPAGKANPSPPVGPALGQRGLNIMEFCKAFNA
                     ATQSMEPGLPIPVVITAFADKSFTFVMKTPPASILLKKAAGLQKGSSNPLTNKVGKLT
                     RAQLEEIAKTKEPDLTAADLDAAVRTIAGSARSMGLDVEGVV"
     gene            complement(14516..15052)
                     /gene="nusG"
                     /locus_tag="WGQ68_001301"
     CDS             complement(14516..15052)
                     /gene="nusG"
                     /locus_tag="WGQ68_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEZ0715093.1"
                     /translation="MSKKWYVVQAYSGFEKNVQRILEERIAREEMGDYFGQILVPVEK
                     VVDIRNGRKTISERKSYPGYVLVEMEMTDDSWHLVKSTPRVSGFIGGRANRPMPISQR
                     EAEIILQQVQTGIEKPKPKVEFEVGQQVRVNEGPFADFNGVVEEVNYERNKLRVSVQI
                     FGRETPVELEFSQVEKIN"
     gene            complement(15054..15332)
                     /gene="secE"
                     /locus_tag="WGQ68_001302"
     CDS             complement(15054..15332)
                     /gene="secE"
                     /locus_tag="WGQ68_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEZ0715094.1"
                     /translation="MTEHTPEKKNVKVDQLVVQDKESASNSGKEGFFAYFSNSWSEFK
                     KVVWPKREDAVRMTVFVIVFVAVLSIFIYAADTAISWLFFDVLLRREG"
     gene            complement(15448..15523)
                     /locus_tag="WGQ68_001303"
     tRNA            complement(15448..15523)
                     /locus_tag="WGQ68_001303"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15488..15490),aa:Trp,seq:cca)
     gene            complement(15530..16078)
                     /locus_tag="WGQ68_001304"
     CDS             complement(15530..16078)
                     /locus_tag="WGQ68_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEZ0715095.1"
                     /translation="MPIEDVFSISGRGTVVTGRVERGIIHVGDEIEIVGLKETQKTTC
                     TGVEMFRKLLDEGQAGDNVGVLLRGTKREDVERGQVLAKPGTITPHTKFKAEVYVLSK
                     EEGGRHTPFFANYRPQFYFRTTDVTGAVTLEEGVEMVMPGENVTITVELIAPIAMEEG
                     LRFAIREGGRTVGAGVVSSVIA"
BASE COUNT         4060 a         4177 c         3601 g         4302 t
ORIGIN      
        1 gctaattcct ttacattaaa gatcgattaa agaacaatgc cgtctgaaaa atcatttcag
       61 cctttcagac gacctatttg cctgattagc ctttacgggc ttcagttaca gcagcagcta
      121 tgtgggcagg agcttcagaa tatttcttga actccataga gtaagtagcg cggccttggg
      181 ttgcagaacg caggtcggtc gaataaccga acatttctgc cagaggtact tcggcacgga
      241 cttttttacc gccgataccg tcatcatcca tacccaatac aacaccgcga cggcggttca
      301 agtcgcccat tacgtcgccc atgtattctt ccggagtttc cacttcaact gccataatcg
      361 gctcaagcag ggcaggagat gcttgacgca taccttcttt aaacgcttga gaagcggcca
      421 attcaaatgc caattgggaa gagtcgacat catggtaaga accgaatacc agacggatac
      481 gcacatcaac tacaggatag ccggcaacga taccgttagg caatgtatcg cggatacctt
      541 tatcgacaga cggaatgaat tcgcgaggaa tcacgccacc tttaatttca tcgataaact
      601 cgtaaccttc gccccccggt tccataggtt ccatttcaat cacaacgtga ccatattgac
      661 ctttaccacc ggattgtttc gcgtgtttgt attcagcttt aacggctttg cggatagttt
      721 cacggtaagc cacttgaggt gcaccgatat ttgcttccac accgaattcg cgtttcatac
      781 ggtcaacaat aatttccaag tgcagctcac ccataccgga aataatggtt tgaccggatt
      841 cttcgtctgt acggacacgg aaagaagggt cttctttagc caagcggttc agggcgatac
      901 ccattttctc ttggtcggct ttggttttcg gctcaacggc aatatggatt accggctcgg
      961 ggaattccat acgttccaag ataatcggcg cgctttccgc acacaaggtt tcaccggtag
     1021 taacgtcttt cagaccaata gcggctgcga tgtcgccggc gcgtacttct tcgatttcag
     1081 tacggtctgc ggcagtcatt tgtaccaaac gaccgatacg ttcgcgagtg ccttttacgg
     1141 agttcaatac ggtatcgccg gattttacta cgcctgagta aacgcggata aaggtcagct
     1201 gaccgacgta tttgtcgttc aacattttga acgccaatgc agagaatttc tcttcatcgc
     1261 tggcttgacg gctgtcggct tcctcggtat tcgggttgac accttgaacc ggaggaatat
     1321 cggtaggagc tggcagcaat tctacaactg cgtccaacat acgttgaaca cctttgtttt
     1381 taaatgcaga accacacagc ataggctgaa tttcgcctgc caaagtacgt tgacgcaacg
     1441 cgcctacgat ttcttcttcg gtcagctcgt cgccgcctaa gtatttgtcc atcagttctt
     1501 cgctggcttc ggctgcggct tcaatcatat tttgacgcca ttcttcggca gtttcgacca
     1561 attcggcagg aatatcgcca taggtaaagg ttgtaccttt atcggcttca ttccaaatga
     1621 tggatttcat tttcaacaaa tcaaccacac cgctgaagtt gtcttccgca ccaaccggaa
     1681 tgacgatagg tacagggttt gcgcgcaaac gggttttcat ttgctcgaca acgcggaaga
     1741 agttggcacc ctgacggtcc attttattga caaacgccaa gcgcggcact tggtatttgt
     1801 tggcttgccg ccatacggtt tcagattggg gttgaacacc gcccaccgcg cagtaaacca
     1861 ttaccgcgcc gtccaataca cgcatagaac gctctacctc tacggtaaag tcaacgtgtc
     1921 ccggggtgtc gatgatgttg aagcggtgct cggggaattg tttcgccata ccggaccagt
     1981 aggaagtaac ggcagcggag gtaatggtaa taccgcgctc ttgctcttgt tccatgtagt
     2041 cggtagtagc cgcaccgtca tgcacttcgc ccagcttgtg ggtcaaacca gtatagaaca
     2101 aaatacgttc tgtcgtcgtg gttttacccg catcgatatg ggcggaaata ccgatgttac
     2161 ggtacaggct gatcggggtc ttacgagcca ttttattagc ctttcaaaat tagaaacgga
     2221 agtgagagaa tgctttgttg gcttcagcca tacggtgtac ttcttcacgt tttttcaacg
     2281 caccgccacg accttcggac gcatcaatca attcgcctgc caaacgcagg tccatggatt
     2341 tctcaccacg tttgcgggcc gcatcgcgaa cccagcgcat tgccaaagcc aaacggcgtg
     2401 aaggacgaac ttcaacagga acttggtagt ttgcaccacc tacacggcgg cttttcactt
     2461 ccacgatagg cttggcgttt gcaatggctt cgttaaatac ttcgattgct actttgccgg
     2521 tttttttctc aatctgttcc aacgcaccgt aaacgatacg ctcggcaacg gattttttac
     2581 cgtcaatcat caatacgttc atgaatttgg tcaactcgac gctgccgaat ttaggatctg
     2641 gcagtacgtc gcgcttgggg acttctctac gtcttggcat tttaatttcc tttaatctat
     2701 tcagtcgggt ctattcccat gaatacccaa ttgagtattc acttactcgg ccgttgttca
     2761 gcttaggcgg ccgacgtgcc tatttaagtc ccagtaatta tttaggacgc ttagcaccgt
     2821 atttggaacg ggcttgttta cggtctttaa cgcctgcagt atccaaagaa ccgcgtacag
     2881 tgtggtaacg cacacctggc aagtctttta cacgaccgcc gcgaatcaat acgacactgt
     2941 gctcttgcag gttgtggcct tcgccgccga tgtatgaaat gacttcaaaa ccgttggtca
     3001 ggcggacttt acatacttta cgcaatgcag agttaggttt tttaggggta gtcgtgtata
     3061 cacgggtgca cacgccgcgt ttttgcgggc aggcttccag tgcgggcact ttgtttacgt
     3121 acacgggctt ttgacggcct ttgcgtacca attggttgat agttggcata ttttctcgtc
     3181 ctgttgagtt aaatacttgc cgacaccatg tcggcaagag tggaattata tttttattgc
     3241 aagcagacag tcaagcgtga gatcaggaag attcatcatt tttctttttt cagataaaca
     3301 tttaggtccc ttatgctcat aaacaactat taccggaaag gaaatcacta ataatttaaa
     3361 tgccgtctga aagaaaaacc acaagcctaa agcttgcggt tttgtattga ataaacgcac
     3421 caaagattat tcatccgtta cttgggtttc agcagtctcc tgctccacct cttgccattg
     3481 ttgatgacgg ctgcggtggt aagtcaaacc ggtaccggca ggaatcaagc gaccgacgat
     3541 gacgttttct ttcaaaccac gcaactcgtc ttgtttgccc ataatcgcgg cttcggtcag
     3601 gacgcgcgtg gtttcttgga acgatgcggc agaaatgaag ctgtcggtgg acagggaagc
     3661 tttggtaata cccagcaata cgttttcgta acgcgccggt tctttgcctt cttccaacgc
     3721 tttttcattg gcaagcatca catcgccgcg ttcgacctgc tctccggtaa tgaacccggt
     3781 ttctccggca tccgcaatgt tcacacggcg caacatttga cggatgatga cttcgatgtg
     3841 cttatcggaa atcttcacac cttgcaggcg gtaaacctct tgcacctctt ggacaatgta
     3901 gcgtgccaat gcttcgatac cttgcaaacg cagaatatcg tgcggatcga ccgcgccgtc
     3961 cacaatggtt tcgcctcggt ttaccacttg accgtcgtgt accagaattt gtttctcttt
     4021 ggaaatcaag gtctcgtatg ctacaccgtc cacgtcagta acaatcagac gttgcttgcc
     4081 tttggtctct ttgccgaagg aaacggtacc ggtaatttcc gccaacatac cggcatcttt
     4141 cggcacgcgt gcttcaaaca attcggcaac gcgcggcaga ccgccggtaa tgtcgcgggt
     4201 tttggaagag gcttgcggaa tacgcgccaa tacgtcgcct ttaccgattt cctgaccttc
     4261 gcgtacggta atcaccgcac caacggggaa tgccatggat accggagtag aagtaccggg
     4321 aatacagatt tccacgccgt tttcgtccaa gagtttcaca gtcggacgca gcagtttgga
     4381 agcactagag gaacgacgtt taccgtcaat caccaccaaa gtggacaaac cggttacatc
     4441 atcggtttgt ttggcaacgg taacgccctc ttccacgttt tcgaatttca ccatacctgc
     4501 gtgttcggta atcatcggac gggtatgcgg atcccaggtt gccaaggttt gaccggcttt
     4561 aatggccata ccgtcttgta ccagcaggat ggcaccgtaa ggtactttgt ggcgttcgcg
     4621 ttcacggccg atatcgtcgt gaatcacgac ttcacaagaa cggccgatga caaccaactc
     4681 gcctttattg ttggcaacat aacgcatttg gctgctgaat cgtgccgtac cgttggattt
     4741 ggcttccact tggctggctg ctgccgcacg ggatgccgca ccaccgatgt ggaacgtacg
     4801 catagtcagc tgggtacccg gttcgccgat tgattgggcg gcaatcacgc cgactgcctc
     4861 tccggcattg accagtttgc cgcgcgccaa gtcgcggccg taacagtgcg cgcacaaacc
     4921 gtggcgggtt ttacaagtaa tcggcgtacg gactttgact tcatcgacac cggattggtc
     4981 aatcatatcc accagttttt cagtcaacaa cgtgcctgct tcaaccaagg tctcgccgct
     5041 tgacggatcg acaacgtcag acgcagtaac acggcccaaa atacgatcgc gcaatgcttc
     5101 aatcacatca ccgccttgta ccactgcctt catgacaaag ccgtctgaag taccgcaatc
     5161 gtcttcaaca acgaccaaat cttgagttac gtctaccaga cgacgggtca ggtaaccgga
     5221 gttcgcggtt ttcaatgcgg tatccgccaa acccttacgc gcaccgtggg tcgcaataaa
     5281 gtattgcaat acggtcagac cttcacggaa gtttgaggta atcggcgttt caataatcga
     5341 gccgtcaggt tttgccatca agccacgcat accggacaac tgtttaatct gagctgcaga
     5401 accacgggca ccggagtccg ccatcatata aatggagttg aatgactctt gatcgacttc
     5461 gttgccgtca cggtcgataa ctttttgttt ggacaagtta tccatcatcg ctttagcgat
     5521 tttatcgccg gcacgacccc aaatatccac tactttgttg taacgttcgc cattggtaac
     5581 caaaccttga cggtattggt cttcgatttc tttaacctcg gcattggctt cagccagcaa
     5641 ggccgctttt tctttcggga tttccatatc gtcaacggca atggaaatac cgccttttgc
     5701 cgcaaatccg aaaccggtgt acatcaggtg gtcagcaaag ataaccgtat cgcgcaagcc
     5761 gcacaggcgg aacgatgcgt taatcagttt agaaatttct tttttcttca atgccttgtt
     5821 gacatattca aacggcaggc ctttcggcag gatttcgctc aacaatgcac ggccgacggt
     5881 tgtttcgtaa cggttaacga caggctcaaa ctcacctgct tcgtttttca cccattcgcg
     5941 cagacgtacg gtgatttttg tacccagctc gacctgtttg gtatggtatg cgcgatgcac
     6001 ttctttcaca tcggcaaaca ggctgccttc gcctttggca ttgatacggt cgcgggtcat
     6061 gtagtacaga cccaatacga tatcttggga aggtacaata atcggttcgc cgttggcagg
     6121 agacaatacg ttgtttgaag ccagcatcag cgtgcgtgct tccatttgtg cttccaagct
     6181 caatggaacg tgtaccgcca tttggtcgcc gtcaaagtcg gcgttgaacg cggcacacac
     6241 caatgggtgc aactgaatcg ctttaccttc aatcaagata ggttcgaacg cttgaatacc
     6301 caaacggtgc agggtcggcg cacggttcag cataatcgga tgttcgcgga tgacttcttc
     6361 caagatgtcc catacttccg gtacttcttg ctctaccaat tttttcgctg ctttaacggt
     6421 agaggccaaa ccttgttttt ccaatttgtg gaaaatgaac ggtttgaaca gttccaaagc
     6481 catttttttc ggcagaccgc attggtgcag acgcaggtat gggcctacgg taatcacgga
     6541 acggccggag tagtccacac gtttgcccaa caggttttga cggaagcgac cgcctttacc
     6601 tttaatcatg tctgccaatg atttcagcgg gcgtttgttg gcgccggtca tggctttacc
     6661 gcgacggccg ttatccaaca gcgagtcaac tgcttcttgc aacatacgtt tttcgttgcg
     6721 gacgataatg tcaggcgcgt gcagttccaa cagacgtttc agacggttgt tacggttaat
     6781 aacgcggcgg tacaaatcgt tcaaatcgga agtggcaaaa cgaccaccat ccaatggaac
     6841 caacggacgc aaatcaggcg gcaataccgg cagcacatcc ataatcatcc attccagttt
     6901 cataccggaa cgatggaagg cttccaatac tttcaagcgt ttggcgattt ttttgatttt
     6961 ggtgtcggat ccggtcgatt ccaactcttg gcgcaggatt tcgatttcgc ccgctacatt
     7021 cagggtacgc agcaattcgc ggataccttc cgcacccatt ttggcatcga aatcgtcgcc
     7081 gtattcgtcc agcttgttgt agtaatcgtc ttcagtcagc aattggcggc gttgcagcgg
     7141 agtcataccg ggatcggtta ccacaaatgc ttcaaagtac aatacgcgct cgatgtcgcg
     7201 caaagtcatg tccagtacca tacccaagcg ggaaggcagg gattttaaga accaaatatg
     7261 tgcgacgggc gcagccaatt cgatatggcc catgcgttcg cggcgcactt tggacagggt
     7321 tacttccacg ccgcattttt cacacgttac gcctttaaat ttcaagcgtt tgtattttcc
     7381 gcacaagcat tcgtagtctt tgaccgggcc aaagattttg gcacagaaca aaccgtcacg
     7441 ctcaggtttg aacgtacggt agttgatggt ttcaggtttt ttgacttcgc cataagacca
     7501 tgagcggatg gtttcgggag aggcaatacc gattttaatg gcatcaaact cttcttccat
     7561 gccggcagtt tgcaacggat taaataagtt caacaaattc atttttgctc cttgaaggaa
     7621 gtatttttac cgcatggcgg attttcgata tggggaaacg gtacttttga tttcagacgg
     7681 cctaaatgcc gattgcttca ttcttattct gaaaccactt tttcagacga ccctgaaagg
     7741 ccgtctgaaa acttttgttt agtaacgttc caaatcgata tccaagccca gtgagcgaat
     7801 ctctttgacc aatacgttga aggactcggg cataccggca tcgattttgt gttcgccttt
     7861 gacgatgttt tcgtacattt tggtacggcc gttcacgtcg tcagacttca cagtcagcat
     7921 ctcttgcagc gtgtaggctg cgccgtatgc ttccagtgcc caaacctcca tctcaccgaa
     7981 acgttggccg ccgaactggg ctttaccgcc caaaggctgc tgggtaacca gactgtacgg
     8041 accggtagaa cgcgcgtgca ttttttcgtc aaccaagtgg tgcagtttca gatagtgcat
     8101 cacacctact gtaaccttgc ggtcaaatgc ttcgcctgaa cggccgtcat acagcgtgat
     8161 ttgggtttta ctgtcgttga agcccagttt ttcaacctca ggatcgtcgc tcggataagc
     8221 caagttcaac atttcgcgga tttcagactc tttcgcaccg tcgaatacag gagaggcgaa
     8281 agatgcacct ttacgcaggt tggaggccag ttcgatgatt tcttcatcag tcagggcatc
     8341 caaatcttct ttcttgccgc tgccgttgta gagtttgttc aagaactcgc gcaactcgcc
     8401 tgctttgcgt tgctctttca gcatacggtc gatacgctcg ccgatacctt ttgctgccca
     8461 acccaagtga acttccaaaa tctgaccgat gttcatacgg gaaggtacgc ccaatgggtt
     8521 cagtacgatg tctaccggac ggccgtccgc catgtaaggc atgtcttcca ctggcagaat
     8581 gcgcgatacc acgcctttgt taccgtggcg gcccgccatt ttgtcgccgg cttgcagacg
     8641 gcgtttgatg gcgataaaaa ctttcaccat tttttgtacg ccgggttgca attcgtcgcc
     8701 ttgggtcagt tttttcttct tgatttcgta taactcgtcc gcttcttcgc gtttttgttg
     8761 caggctcact ttaatcagtt ccaactgctt ggccaaatct tcatcggtca gacggatatc
     8821 gaaccaatcg tgcctgctcg gcagacccgc caaatattcg gtagtaattt cgctgccttt
     8881 agccagcttc atcggaccac cattggcttt ttgacccaca atcatacgtt cgatacggtc
     8941 gaatgcgtcg ttgtcgaaaa tacgcaattg gtcgttcaaa tccaaacggt agcgtttcaa
     9001 ctcggaatcg ataatggatt gggcgcgttt gtcgcgttga ataccttcac gagtgaagac
     9061 ttgaacgtcg ataacggtac cgctcatgcc ggtaggcata cgcaatgaag tatcttttac
     9121 gtcagatgct ttttcaccga agatggcgcg cagcagtttt tcttccggcg tcagttgggt
     9181 ttcgccttta ggcgttacct tgcctaccaa cacatcgccg gcttctactt ccgcaccgat
     9241 gtaaacgata ccggattcgt ccaaacggtt ttgcatacgc tcggacaagt tcggaatatc
     9301 gcgggtaatg tcttccgcac ccagcttagt atcgcgggca acgacattca attcctcaat
     9361 gtgaatcgaa gtatagcggt cgtccgcagc cactttttcg gaaatcagaa tcgagtcttc
     9421 gtagttgtaa ccgttccacg gcatgaaggc gatggtcatg ttttgaccca aagccaattc
     9481 gcccaaatcg gtggacgcgc cgtcggccac caaatcgccg cgttgcaaaa catcgccggc
     9541 tttgacggca ggacgctgat tgatattggt agactggttg gaacgggtga atttaaccaa
     9601 gttgtaaata tcgacaccca cttcaccggc agtcgcttcg tcgtcatgga cacggatcac
     9661 aacgcggttg gcatcgacat actcgaccac gccgcctcgg cgggcaacga ttgcagtagc
     9721 agagtcaacg gcaacggaac gctcgatacc ggtaccgacc atcggttttt caggacgcaa
     9781 gcaaggcact gcctgacgtt gcatgttggc acccatcaat gcgcggttcg cgtcatcatg
     9841 ttccaagaat ggaatcaggg atgctgcaac ggataccact tgaccagttg ccacgtccat
     9901 atattggacg cggtcgggcg ttgccataat ggtttcgcct ttttcacgac aggtgaccaa
     9961 atcgccaatc agattgccat ctgaatccaa atcggcattc gcctgtgcaa tcacatagcg
    10021 gccttcttcg atggcagaca agtaatcgat ttcctcggtt actttgccgt cgataacgcg
    10081 gcggtaaggc gtttccaaga aaccgtaatc attggtgcgc gcataaacgg acaatgagtt
    10141 gatcaaaccg atgttcggac cttcaggcgt ttcaatcgga catacgcgac cgtagtgggt
    10201 cggatgcacg tcccgcacct caaatcctgc acgttcgcgg gtcaaaccgc ccggacccaa
    10261 tgcggatacg cggcgtttat gggttacttc agacaaggga ttggtctgat ccataaactg
    10321 actcaattgg ctggagccga agaattcttt aatagcagca gaaacaggtt ttgcattaat
    10381 caaatcgtgc ggcatcaagt tttctgattc cgcctgattc aaacgttctt ttacggcacg
    10441 ttccacacgg gccaaaccgc tacggaattg gttttcagtc agctcgccta ccgaacgtac
    10501 tcggcggttg cccaagtgat cgatatcgtc cacttcgcca tggccgttac gcaactcgac
    10561 caaagtcgca atcgaggcga caatatcttc gacgctcagg acataaccgc ctttgtcggc
    10621 agcaccggca aacgtttcgt tcagcaggcg gccgtaccaa gagttttgtt gggcttcgga
    10681 cagtttttgt tcgtatgtgc gcgtattaaa tttcatacgg cctacgcggg acaaatcgta
    10741 gctgtcttca ctgaagaaca agcggttaaa caattgctcg accgcctctt cagtgggcgg
    10801 ttcgcccgga cgcatcatac ggtaaatcgc aacacgcgcc gcctgccggc cggcagtctc
    10861 atccgtacgc aaggtattgg agatataagc accctgatcc aactcattga tgtaaagggt
    10921 cgtaatttct tttacgccgt tgatatcaaa tttggccaac aactcttctg taatttcatc
    10981 attggcagaa gccaatacct cgccggtttc cgaatcaatc agatcggcag ccaatgcttt
    11041 gcccagcagg ctttctggtt ctacatccaa acgggtcagg cctgcattgg taatatcacg
    11101 gatatttttc gcagtaatgc gcttaccttt ggcaaccaat acattgcctt ctttatccaa
    11161 gatatcgacc ttggcagttt cgcctttcag acggcctgcg accaaatcgg tttgaacacc
    11221 gtttgaagac aaatagaacg tttctttgtc gtagaaaata tccaagattt gctcattgtt
    11281 gtagcccaaa gccttcaaca aaatcgttac cggcatttta cggcggcggt cgatacggaa
    11341 atacagcaaa tctttcggat caaattcaaa atccaaccat gaaccacggt agggaatgat
    11401 acgggcggag aataacaact tgccggaaga gtgcgtctta cctttgtcat gctcgaagaa
    11461 tacgccgggc gaacggtgca actgggagac aatcacacgc tctgtgccgt taatcacaaa
    11521 agaaccgctc ggggtcatca acggaatttc gcccatatac acttcgtttt cacgaacttc
    11581 ttttaccgtc ggtttagatg cttccttatc caaaatcacc aaacggatac gcgcgcgcaa
    11641 gggggctgca taagtgattc cgcgcaactg acattcggga atatcgaaca aaggctcgcc
    11701 caatgtgtaa tgcacaaact ccaatcgcgc ataaccgtta tggctcacaa tcgggaaaat
    11761 agaattaaat gccgcctgca gaccgtcatc ggtacgtttg tcaaaagcat tttccagctg
    11821 caaaaacttc gcataagaat caatttgggt tgctagcaag aaaggaactt ccaaaacatt
    11881 ttcccgcttt gcaaaactct tacggatacg ttttttctcg gtaaacgaat agttcataca
    11941 cactccgaaa agtaattttc aataataggc cgtctgaaac aacggtacgc atcaatctga
    12001 tatttacatt tatttgcaat gacttgataa aaactatgca aataaatgta aacaatactt
    12061 gatgtttatt ttaagaagca aaataaggct ggcagaaaac tgccagcctt cataagaagc
    12121 atcaaattat ttgatttcga ctttagcgcc tgcttcttcc agttgttttt ggatgtcttc
    12181 ggcttcagct ttagaaacac cctctttaat ggttttaggt gcgccgtcaa cgatgtcttt
    12241 agcttctttc agacccaaac cggtaattgc acggacaact ttaatcacgc cgactttttg
    12301 atcaccggca gaagccaaaa cgacatcaaa ttcggttttt tcttcagcat cggcagcacc
    12361 ggcaccagca ggacctgcaa ctgcaacagc agcagcagaa acaccgaatt tttcttcaaa
    12421 agctttaacc aagtcgttca attccattac ggtcaaagaa ccaactgctt ccaaaatgtc
    12481 ttctttagta atagccatgc tattttactc caaatattgt attaaaaaat aattgattaa
    12541 cagaaacaaa atcgattaag cggcttcttc gccggctttt ttctctgcca aagcagccaa
    12601 accgcgcgca aagcccgata caggagcttg cataacgaac aacagtttgg acaacagctc
    12661 ttcgcggctc ggaatagaag ccaactcagc aacctgagca gcattcatta cttcgccatt
    12721 gtaagaaccg gctttaacga caattttgtc atcttttttc gcgaattggt gcaacacttt
    12781 agcagcagca acagcatctt cagaagcagc gtaaaccaac ggaccaacca tttgatcggc
    12841 caattctgca aatgaagtac cttgcactgc acgacgagcc aaagtatttt tcagaacgcg
    12901 caaataaacg ccttctttac gtgcattcgc acgaagctca gtcatactgg aaacactgat
    12961 accgcgatat tcagcgacta cgagggtttg agcattagca attgccgcgc taatttcctc
    13021 gaccgccact ttcttggttt caatattgag actcaaggtc tacctcccac tgtttataaa
    13081 caggatacca aaacgatatc ccaccagcgc ggtaacctaa aaagacatct tacaagcaat
    13141 cttgcaatgt gtttcaggac taccgtctgc gtagggcagt tacgattaaa tcttacgatc
    13201 cctacggtct tggacatcta cttaatttta agtagcccaa attcaagcag cccagaaaac
    13261 caaactgctt gaacattcct taagattagt tatttacgct tgatgtatca acgcgaatac
    13321 ccaaacccat ggtgctagac acagcaactt ttttcagata ctgaccttta gcggcagcag
    13381 gcttggcttt aacgatagca tccagcaacg catcaaagtt ctctttcaaa tcagcttcag
    13441 cgaatgaagc acgaccaatc gttgcatgaa caatacctgc cttgtcggta cggtactgca
    13501 cttgacctgc ttttgcattt tttactgctt cggcaacatt aggggtaacc gtacctactt
    13561 tagggtttgg catcaagcct cgaggaccca aaatagtacc caactgacca acaatacgca
    13621 ttgcatcggg agaagcaata acaacatcaa agtttagatt gcctgctttg atttcagcag
    13681 ccaaatcttc gaaaccgacg atatctgcac cagcttcttt agcagcttct gcatttgcac
    13741 cttgagtaaa tacagccaca cgggttgtct taccggtacc tttaggcaga acgactgaac
    13801 cacggataac ttggtcagat ttacgcggat caacgcccaa gttgaaagat acgtcaacag
    13861 actcgtcaaa tttagcagtc gctgcttttt ttaccaaagc aattgcttca tcaattgcat
    13921 ataatttatt ggcttccaca gaagagcgaa gagctttcaa acgtttagat actttggcca
    13981 ttatacaaca ccctccacgt ccaagcccat tgagcgagca gaacctgcta tagtacgaac
    14041 agccgcatcc aagtcagcag cagtcaaatc aggctcttta gttttagcaa tttcttccaa
    14101 ctgagcacgg gtcaatttac ccactttatt ggtcagagga ttagaactac ctttttgcaa
    14161 accggcagct tttttcaaca agatagaagc tggcggggtt ttcatcacaa atgtgaacga
    14221 tttatctgca aatgcagtaa tcacaaccgg aatcggcaag ccaggctcca tactttgggt
    14281 tgcagcattg aatgccttac aaaattccat aatattcaaa ccgcgctgac ccaaagcggg
    14341 accaaccgga ggagatggat tggctttacc tgcaggaatt tgcagtttaa tatagccgat
    14401 aattttcttt gccacttaac ggactcctaa aaacgggtaa gacgcggaac catttccgct
    14461 ccccaagtta atttttgatt ttatcctctt attgcttttt tttcaagtat aaaaatcagt
    14521 taatcttttc aacctggctg aactccagct caacgggtgt ttctctacca aatatctgaa
    14581 cagacacgcg taacttattc cgttcataat tgacctcctc aaccacaccg ttgaaatccg
    14641 caaacggccc ttcatttaca cgaacctgtt gaccgacctc aaattcaact tttggtttcg
    14701 gcttctctat gccggtctga acctgctgta aaataatttc agcctctctc tgactaatcg
    14761 gcatgggtct attagccctc cctccaataa aaccggaaac acggggggtg ctttttacaa
    14821 gatgccaaga gtcatctgtc atttccatct caactagcac ataaccagga tatgactttc
    14881 tttcactaat agtcttacga ccattgcgga tatcaacaac tttctctaca ggcaccagaa
    14941 tttgtccgaa ataatctccc atctcctcac gggcaatgcg ctcttccaat attcgttgga
    15001 cattcttctc aaaccccgaa tacgcctgta caacatacca ttttttcgac atctcaacct
    15061 tcccttctca gcaatacatc aaaaaataac cacgaaattg ctgtatctgc cgcatagata
    15121 aatatagaaa gcacagcaac aaacactata acaaatacag tcattctgac agcatcttca
    15181 cgcttaggcc aaaccacctt tttgaattcg gaccaagaat ttgagaaata tgcaaaaaac
    15241 ccttccttac cggaattaga tgcagattct ttatcttgaa caaccagttg atccacttta
    15301 acgttctttt tttcaggcgt atgttctgtc atattattta tccatcatag catctgtcta
    15361 ttctcaatcc atgtaaatgg caagagagtt tactaaataa caaatacaaa aaaaattaac
    15421 cggcacaagg ccggttaatt ttttatttgg caggccaaga gggtctcgaa cccccaaccc
    15481 tcggttttgg agaccgatac tctaccaatt gagctattgg cctctaaact taagcgataa
    15541 cagaagaaac cacgccggca cccacggtac ggccgccttc gcgaatcgca aagcgcaggc
    15601 cttcttccat agcgataggc gcaatcagtt ctacggtaat ggttacgttc tcgcccggca
    15661 ttaccatttc cacaccttct tccaaagtaa ccgcgccggt tacgtcggtg gtacggaagt
    15721 agaattgggg acggtagttg gcgaagaacg gggtatggcg gccgccctct tctttgctca
    15781 gtacgtatac ttctgctttg aacttggtgt gaggagtgat tgtacccggt ttagccaata
    15841 cttgaccacg ctctacgtct tcacgtttgg taccgcgcag caatacgcct acgttgtcgc
    15901 ctgcttgacc ttcgtccagc agtttgcgga acatttcaac accggtacaa gtggtttttt
    15961 gagtttcttt cagaccgacg atttcgatct cgtcaccgac gtggatgata ccgcgctcta
    16021 cacgaccggt tactactgta ccacgaccag aaatagagaa tacgtcttcg ataggcaaca
    16081 agaaaggttt gtccacggca cgctcgggag tcgggatgta gctgtccaat gcagcagcca
//
LOCUS       DAWFHY010000048        16110 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.048, whole genome shotgun sequence.
ACCESSION   DAWFHY010000048 DAWFHY010000000
VERSION     DAWFHY010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 16110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16110
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.048"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(339..761)
                     /locus_tag="WGQ68_001305"
     CDS             complement(339..761)
                     /locus_tag="WGQ68_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEZ0715096.1"
                     /translation="MQVTSKWIDGMCFVGTTEGGHSVVMEGSAAEGKAKRGPSPLEML
                     LLGVAGCSSIDVVMIAEKQRQKVTDCRATVTAKRADDAPRVFTEIHIHFKVFGHDLKE
                     SAIERAVQMSAEKYCSASIMLGKAAKITHSFEIAGADK"
     gene            complement(879..1550)
                     /locus_tag="WGQ68_001306"
     CDS             complement(879..1550)
                     /locus_tag="WGQ68_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEZ0715097.1"
                     /translation="MMKTFKNIFSAAILSAALPCAYAAGLPQSAVLHYSGSYGIPATM
                     TFERSGNAYKIVSTIKVPLYNIRFESGGTVVGNTLHPTYYRDIRRGKLYAEAKFADGS
                     VTYGKAGESKTEQSPKAMDLFTLAWQLAANDAKLPPGLKITNGKKLYSVGGLNKAGTG
                     KYSIGGVETEVVKYRVRRGDDAVMYFFAPSLNNIPAQIGYTDDGKTYTLKLKSVQING
                     QSAKP"
     gene            complement(1566..2192)
                     /locus_tag="WGQ68_001307"
     CDS             complement(1566..2192)
                     /locus_tag="WGQ68_001307"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEZ0715098.1"
                     /translation="MKNIVILISGRGSNMQAIVNAAIPNVRIAAVLSNSETAAGLQWA
                     AGQGIPTDSLNHKNFESRLAFDTAMMEKIDAYQPDLVVLAGFMRILTPEFCAHYEGRL
                     MNIHPSILPSFTGLHTHERSLEAGCRVAGCTIHFVTAELDCGPIVSQGIVPILDGDTA
                     DDIAARVLAVEHKLYPKAVADFAAGRLIIEGNRVRNSENADAARFLTA"
     gene            complement(2299..3117)
                     /locus_tag="WGQ68_001308"
     CDS             complement(2299..3117)
                     /locus_tag="WGQ68_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017628850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEZ0715099.1"
                     /translation="MNTIFKISALTLSAALALSACGKKEAAPASASEPAAASSAQDDT
                     SSIGSTMQQASYAMGVDIGRSLKQMKEQGAEIDLKVFTEAMQAVYDGKEIKMTEEQAQ
                     EVMMKFLQEQQAKAVEKHKADAKANKEKGEAFLKENAAKDGVKTTASGLQYKITKQGE
                     GKQPTKDDIVTVEYEGRLIDGTVFDSSKANGGPVTFPLSQVIPGWTEGVQLLKEGGEA
                     TFYIPSNLAYREQGAGDKIGPNATLVFDVKLVKIGAPENAPAKQPAQVDIKKVN"
     gene            complement(3376..3816)
                     /locus_tag="WGQ68_001309"
     CDS             complement(3376..3816)
                     /locus_tag="WGQ68_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEZ0715100.1"
                     /translation="MPKATFYTHVAQVPIFTCRLIARAIRDGGRILVWSDSFGQLQEL
                     DKMLWQYEAESFIPHEIWETEEAMPSEPSVLLACGGNLPRIPEGMTVLNLSDGFWNTA
                     PVLPARVLEIVGNSLEDLADARERFTAYRRSGFAIEHHGMEGKA"
     gene            complement(3879..5285)
                     /locus_tag="WGQ68_001310"
     CDS             complement(3879..5285)
                     /locus_tag="WGQ68_001310"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEZ0715101.1"
                     /translation="MEFSTKTEILQEQQAGAQLFVCTEEAQLNHPTALALLSSLEEGQ
                     NFADTKIPTGNGLQAVAVCCLKSTGRAALNKAAAEAAKWAQNQETVNVDVHVFEEAQA
                     AAVAEAFAIAFGNAAYRFDRYKKEAKPAKFETAVFHTAHEAAVKEALRVAEAQVYGQS
                     LCRDLGNAAPNECTPEFLARTAKAEAEKLGAHAKIIEKDYIKENMGSFWSVAKGSVED
                     PYLVELSYFGAADKEAAPVVLVGKGITFDTGGISLKPGLNMDEMKFDMCGAATVISTF
                     CAAVKLQLPINLVAVVATCENMPSGAANKPGDVVKSMKGLTIEVLNTDAEGRLILCDA
                     LTYAEQFKPKAVIDVATLTGACIVALGHDVSGVMGNNQDLIDSLLAASRNVDDKAWQL
                     PLFETYKDQLKSNFADIPNIGTPGAGTITAATFLSYFTEGYPWAHLDIAGTAWKSGAE
                     KGATGRPVPLLLNYLRNL"
     gene            5455..6570
                     /gene="lptF"
                     /locus_tag="WGQ68_001311"
     CDS             5455..6570
                     /gene="lptF"
                     /locus_tag="WGQ68_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEZ0715102.1"
                     /translation="MIYQRNLIKELSFTAVGIFVVLLAVLVSTQAINLLGRAADGRVA
                     IDAVLALVGFWVIGMTPLLLVLTAFISTLTVLTRYWRDSEMSVWLSCGLALKQWIRPV
                     MQFAVPFAVLVAVMQLWVIPWAELRSREYAEILKQKQELSLVEAGGFNSLGKRNGRVY
                     FVETFDTESGIMKNLFLREQDKNGGDNIIFAKEGNFSLNDNKRTLELRHGYRYSGTPG
                     RADYNQVSFQKLNLIISTTPKLIDPVSHRRTIPTAQLIGSSNPQHQAELMWRISLTVS
                     VLLLCLLAVPLSYFNPRSGHTYNILIAIGLFLIYQNGLTLLFEAVEDGKIHFWLGLLP
                     MHIIMFVIAIVLLRVRSMPSQPFWQAVGKSLTLKGGK"
     gene            6567..7637
                     /gene="lptG"
                     /locus_tag="WGQ68_001312"
     CDS             6567..7637
                     /gene="lptG"
                     /locus_tag="WGQ68_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010358669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEZ0715103.1"
                     /translation="MNLISRYIIRQMAVMAVYALLAFLALYSFFEILYETGNLGKGSY
                     GIWEMLGYTALKMPARAYELMPLAVLIGGLVSLSQLAAGSELAVIKASGMSTKKLLLI
                     LSQFGFIFAIATVALGEWVAPTLSQKAENIKAAAINGKISTGNTGIWLKEKNSIINVR
                     EMLPDHTLLGIKIWARNDKNELAEAVEADSAVLNSDGSWQLKNIRRSTLGEDKVEVSI
                     AAEENWPISVKRNLMDVLLVKPDQMSVGELTTYIRHLQNNSQNTRIYAIAWWRKLVYP
                     AAAWVMALVAFAFTPQTTRHGNMGLKLFGGICLGLLFHLAGRLFGFTSQLYGIPPFLA
                     GALPTIAFALLAVWLIRKQEKR"
     gene            7947..10532
                     /gene="acnB"
                     /locus_tag="WGQ68_001313"
     CDS             7947..10532
                     /gene="acnB"
                     /locus_tag="WGQ68_001313"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003679951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HEZ0715104.1"
                     /translation="MLEAYRKAAAERAALGIPALPLNAQQTADLVELLKSPPAGEGEF
                     LVELLAHRVPPGVDDAAKVKASFLAAVAEGSASSPLISPEYATELLGTMLGGYNIHAL
                     IELLDDDKLAPIAAKGLKHTLLMFDSFHDVQEKAEKGNKYAQEVLQSWADAEWFASRA
                     KVPEKITVTVFKVDGETNTDDLSPAPDAWSRPDIPLHALAMLKNPRDGITPDKPSEVG
                     PIKLLEELKAKGHPVAYVGDVVGTGSSRKSATNSVIWHTGEDIPFVPNKRFGGVCLGG
                     KIAPIFFNTQEDSGALPIEVDVSALKMGDVVDILPYEGKIVKNGETVAEFELKSQVLL
                     DEVQAGGRINLIIGRGLTAKAREALKLPASTAFRLPQAPAESKAGFTLAQKMVGRACG
                     LPEGQGVRPGTYCEPRMTTVGSQDTTGPMTRDELKDLACLGFSADMVMQSFCHTAAYP
                     KPVDVKTHKELPAFISTRGGVSLRPGDGVIHSWLNRLLLPDTVGTGGDSHTRFPIGIS
                     FPAGSGLVAFAAATGVMPLDMPESVLVRFSGKLQPGVTLRDLVNAIPLYAIKQGLLTV
                     AKAGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTVKLNKEPIIEYMKSN
                     VVLMKNMIANGYQDPRTLERRIKAMEKWLANPELLEADKDAEYAAVIEINMDDIKEPI
                     IACPNDPDDVCFMSERSGTKIDEVFIGSCMTNIGHFRAASKLLEGKADTPVRLWIAPP
                     TKMDAKQLSDEGHYGVLGRAGARMEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLG
                     KNTFVYLGSAELAAICSKLGKIPTVEEYQANIGIINEQGDKIYRYMNFNEIDSYNEVA
                     ETVNV"
     gene            10674..11669
                     /locus_tag="WGQ68_001314"
     CDS             10674..11669
                     /locus_tag="WGQ68_001314"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEZ0715105.1"
                     /translation="MNLKNRHFLKLLDFTPEEITAYLDLAAELKAAKKAGREIQRMKG
                     KNIALIFEKTSTRTRCAFEVAARDQGAGVTYLEPSASQIGHKESIKDTARVLGRMYDA
                     IEYRGFGQEVVEELAKYAGVPVFNGLTNEFHPTQMLADALTMREHSGKPLNQTAFAYV
                     GDARYNMGNSLLILGAKLGMDVRIGAPQSLWPSEGIIAAAHAAAKETGAKITLTENAH
                     EAVKNVDFIHTDVWVSMGEPKEVWQERIDLLKDYRVTPELMAASGNPQVKFMHCLPAF
                     HNRETKVGEWIYETFGLNGVEVTEEVFESPASIVFDQAENRMHTIKAVMVAALGD"
     gene            complement(11738..11839)
                     /locus_tag="WGQ68_001315"
                     /pseudo
     CDS             complement(11738..11839)
                     /locus_tag="WGQ68_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(11893..12906)
                     /gene="ilvC"
                     /locus_tag="WGQ68_001316"
     CDS             complement(11893..12906)
                     /gene="ilvC"
                     /locus_tag="WGQ68_001316"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEZ0715106.1"
                     /translation="MQVYYDKDADLSLIKGKTVAIIGYGSQGHAHAANLKDSGVNVVI
                     GLRQGSSWKKAEAAGHVVKTVAEATKEADVVMLLLPDETMPAVYHAEVAANLKEGATL
                     AFAHGFNVHYNQIVPRADLDVIMVAPKGPGHTVRSEYKRGGGVPSLIAVYQDNSGKAK
                     DIALSYAAANGGTKGGVIETTFREETETDLFGEQAVLCGGVVELIKAGFETLTEAGYA
                     PEMAYFECLHEMKLIVDLIFEGGIANMNYSISNNAEYGEYVTGPEVVNASSKEAMRNA
                     LKRIQTGEYAKMFIQEGNVNYASMTARRRLNADHQVEKVGAQLRAMMPWITANKLVDQ
                     DKN"
     gene            complement(12986..13279)
                     /locus_tag="WGQ68_001317"
     CDS             complement(12986..13279)
                     /locus_tag="WGQ68_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004521031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEZ0715107.1"
                     /translation="MSNIKIVALVTVKPEYTETLVAQFKELVKASRAEEGNISYDLHQ
                     EIGKPNRFVFVENWKSQAAIDEHNASAHFQAFVQSVDGKTEALEIVLMNEVAA"
     gene            complement(13346..13837)
                     /gene="ilvN"
                     /locus_tag="WGQ68_001318"
     CDS             complement(13346..13837)
                     /gene="ilvN"
                     /locus_tag="WGQ68_001318"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEZ0715108.1"
                     /translation="MRHILSVLIENESGAMSRVVGLFSARDYNIDSLAVAPTEDKTLS
                     RMTIVTHGDEQVIEQITKQLNKLIEVIKVVDLNESRFVERELMLVKVRAAGKDRDEFL
                     RLTEIYRGSIIDVTDRSYTIEITGSTDKLDSFLETVGRAQILETVRTGAAGIGRGERI
                     LKI"
     gene            complement(13848..15575)
                     /gene="ilvB"
                     /locus_tag="WGQ68_001319"
     CDS             complement(13848..15575)
                     /gene="ilvB"
                     /locus_tag="WGQ68_001319"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HEZ0715109.1"
                     /translation="MQLSGAQIIVQSLKAEGVEYVFGYPGGAVIEIYDALFQLNKFKH
                     ILTRHEQAAVHAADAYARVSGKVGVALVTSGPGVTNALTGIATAYTDSIPMVVISGQV
                     GNSLIGTDAFQEVDTVGITRPCVKHNFLVTDINELAETIKKAFQIAASGRPGPVVVDV
                     PKDVTQAMAKFSYPQEDIFIRSYQPVVQGHIGQIKKAVQMLASAKRPVVYFGGGVVLG
                     NASEELTRFVRMTGAPCTGTLMGLGAYPSGDRQFLGMLGMHGTYEANLAMQNADVVLA
                     VGARFDDRVVSVPSKFFEKAKKVIHIDVDPSSIAKRVKADIPIVGDVKNVLSEMVALW
                     QKQESVPSEDALGKWWKTIEEWRSRDCLWFDNGSEIIKPQYVIQKLAEITGNSAIITS
                     DVGQHQMFAAQYYPFERPRQWLNSGGLGTMGVGLPYAIGAKLAAPDQDVFCITGDGSI
                     QMNIQELSTCFQYRIPVNVITLNNGYLGMVRQWQEIYYGGRESETYFDSLPDFVKLAE
                     AYGHIGIRVDKKSDVEGALLEALNQKDRLVFIDFLTDQKQNVMPMVGNGKGLDEMVLP
                     PHMRADEKA"
BASE COUNT         3783 a         4366 c         4081 g         3880 t
ORIGIN      
        1 cgtactggtt tttgttaatc cactataatt caataaatta atatatggct taaaataacg
       61 ggattctcgc ctcccgcccg cccgcagaag caggcgggta tcattttaaa acgcggcatt
      121 taaaatttga ccgaaaattg ttgacaatcc ggaatcaagt ctgcacaata ccccgacaag
      181 tccaagtatt ataaaggctg aataaagagg aaacagcagg cagatatatt cgggaggtgc
      241 agtccgaata tatctgcttt tttatgcgcc tccggattgc ctgccgcacc tttcccttca
      301 gacggcatca gccgtttccc cataatgccg cccgatgcct atttatctgc cccggcaatt
      361 tcaaaactgt gggtaatctt tgccgctttg cccaacataa tcgaagccga acagtatttt
      421 tcggcagaca tctgaacggc gcgctcaatg gccgattctt tcaaatcatg cccgaatact
      481 ttgaaatgga tgtggatttc ggtaaacacg cgcggcgcat cgtccgcccg tttcgccgta
      541 accgtcgcac ggcagtcagt cactttctga cgctgttttt cggcaatcat caccacatcg
      601 atgctcgaac agcccgccac gcccaacagc agcatttcca aagggctggg cccgcgctta
      661 gccttacctt ctgccgccga cccctccata acgacgctgt gcccgccttc cgttgttccg
      721 acaaagcaca ttccgtctat ccattttgat gtaacctgca tggtgtcatt cctgaaaata
      781 gcgttaaaac cgctttgcat atggcgttat tgtaaacaat ttcaagcggc ttatgcagaa
      841 atatggacaa aacggcaaaa aaacgcctga aaatcgattt acggtttggc ggactggcca
      901 ttgatctgca ccgatttgag tttcagcgta taggttttgc cgtcgtcggt atagccgatt
      961 tgtgccggaa tattgttcag ggacggtgcg aagaaataca ttaccgcatc gtcgccgcgc
     1021 cgcacccgat atttgacgac ttcggtttcc acgccgccta tgctgtattt tcctgtaccc
     1081 gccttattca aaccgccgac ggaataaagt tttttgccgt tggtgatttt cagccccggg
     1141 gggagtttcg cgtcatttgc cgccaactgc caggcaagcg tgaacaaatc catagccttg
     1201 gggctttgct cggttttgct ctcgcccgct ttgccgtaag ttacgctgcc gtcggcgaat
     1261 ttggcttccg catacagttt gcccctgcgt atgtctctat agtaggtagg gtgcagggta
     1321 ttgccgacaa ccgtaccgcc ggactcgaaa cggatattgt atagcggcac tttaatcgtc
     1381 gaaacgattt tgtaagcatt gccgctgcgt tcaaatgtca tcgtggcggg aatgccgtag
     1441 ctgccggaat agtgcagcac ggcggattgg ggcagccctg ccgcatacgc gcacggcagg
     1501 gcggcggaca aaatggcggc ggaaaatata tttttaaaag tcttcatcat ttgctcccgc
     1561 ccggtttacg ccgtcaggaa acgggcggca tcggcgtttt ccgaatttct gacgcggttt
     1621 ccctcgataa tcaggcggcc ggcggcaaaa tcggcaacgg ctttcggata gagtttgtgt
     1681 tcgacagcca aaacccgggc ggcaatatcg tctgccgtat caccatcaag tatcggcaca
     1741 atcccttgcg atacaatcgg gccgcaatcc agctcggccg taacaaagtg aatcgtacag
     1801 cctgcaacgc ggcaacccgc ttccaaagag cgttcgtgcg tatgaagccc agtaaatgaa
     1861 ggcaggatgg acggatggat gttcatcagc ctgccttcgt aatgggcgca aaactcgggg
     1921 gtcagaatcc gcataaaacc tgccaaaacc accaagtcgg gttgatatgc gtcgattttt
     1981 tccatcatgg cggtatcgaa ggcaagtcgt gattcaaagt ttttatgatt caggctgtcg
     2041 gtcggaatac cctgaccggc cgcccattgc aaaccggcag ccgtttcgct gttgctcaac
     2101 acggcggcaa tacggacgtt gggaatggcg gcattgacga ttgcctgcat attgctgccg
     2161 cgtccggaaa tcaggatgac gatgtttttc ataatggtgc gcttttgaaa gggatgccat
     2221 ctgaaccgct gtttggtggt ttcagacggc atttgccgta aaaatgcccg aaaacctgtt
     2281 tcgggcatgg attcggactt aatttacttt tttgatgtcg acttgagccg gctgcttggc
     2341 gggcgcgttt tcgggtgcgc cgattttgac cagtttcaca tcaaatacca aagtggcgtt
     2401 cgggccgatt ttgtcgcccg caccctgttc gcggtaagca aggttggacg ggatgtagaa
     2461 cgtggcttcg ccgccttctt tcagaagctg tacgccttcg gtccaacccg gaatcacttg
     2521 gctcaaaggg aaggtgaccg ggccgccgtt ggctttgctg ctgtcgaata ccgtaccgtc
     2581 aatcaggcgg ccttcgtatt ccacggtaac gatgtcgtct ttggtcggct gtttgccttc
     2641 accctgtttg gtgattttgt attgcaggcc ggaagcagtg gtcttcacgc cgtctttggc
     2701 ggcattttct ttcagaaagg cttcgccttt ttctttattg gccttcgcgt ccgccttgtg
     2761 tttttctacg gctttagcct gttgttcctg aaggaatttc atcatgactt cctgagcctg
     2821 ctcttcagtc attttgattt ctttgccgtc atacactgcc tgcatggctt cggtaaagac
     2881 tttcaaatcg atttccgcgc cctgttcctt catttgcttc agggagcgtc cgatgtccac
     2941 gcccatcgca tagcttgcct gctgcatcgt gctgccgatc gaagaggtgt cgtcctgcgc
     3001 ggaagaagcg gcggcaggtt cggatgcaga tgcgggggcg gcttcttttt tgccgcaggc
     3061 ggaaagtgcc aaagcggcgg aaagggtcag tgcgctgatt ttgaaaatgg tgttcatgat
     3121 ggatcttcgc tgtcgataag gtcggaaaaa cgggattata gccgagtttg aatgtttcaa
     3181 cacacaggat gacacataaa gcgtcaatcg tgtgttgccc tgttttggaa gggattgaac
     3241 cttccaaaat aagttttgat tctaccgccc cgagggacag atgtccaagt ggcggggttc
     3301 aaccgataag gaaattttaa tcaaatagaa tcaagcctgt ttaaattttg taaatgcggc
     3361 atttcagacg gcattttatg ccttgccctc catgccgtga tgttcgatgg caaaaccgct
     3421 tcggcggtag gcggtaaagc gttcgcgcgc gtcggcgaga tcttccaagc tgttgccgac
     3481 gatttccaaa acgcgcgcgg gcaagaccgg agcggtgttc caaaaaccgt cagacaggtt
     3541 caaaacggtc atgccttcgg gaattcgggg cagattgccg ccgcaggcaa gcaggacgga
     3601 tggttcagac ggcatggctt cttccgtttc ccaaatttcg tgcggaatga aactctcggc
     3661 ttcgtattgc caaagcattt tgtccaattc ctgaagctgc ccgaacgaat cggaccacac
     3721 cagtatcctg ccgccgtccc gaatcgcacg ggcaatcagg cggcaggtga aaatcggaac
     3781 ttgggcaacg tgcgtgtaaa aggtggcttt cggcatattg tttgaacatt tggtaggata
     3841 atgccgtctg aaaggcttca gacggcattg tggcaaaatt aaagattccg cagatagttc
     3901 agcagcaagg gaacgggacg gccagtcgca cctttttccg caccggattt ccacgccgta
     3961 cccgcgatgt caaggtgcgc ccacgggtag ccttcagtaa agtaagacag gaaggtggcg
     4021 gcggtaatcg tgcccgcgcc gggcgtgccg atattgggga tgtcggcaaa gttggatttg
     4081 agctggtctt tgtaggtttc aaagagcggc agttgccacg ctttgtcgtc cacgttgcgg
     4141 gaggcggcga gcaggctgtc gatcaaatcc tgattgttgc ccatcacgcc gctgacatcg
     4201 tgccccaagg cgacgatgca tgcaccggtc agggtggcga cgtcgatgac ggctttgggt
     4261 ttgaactgtt cggcgtaagt gagcgcgtcg cacaaaatca gacggccttc agcatcggtg
     4321 ttcaacactt cgatggtcag acctttcata cttttcacga catcgcccgg tttatttgcc
     4381 gcgccggaag gcatgttttc acaagtggcg acgacggcga cgaggttaat cggcagttgc
     4441 agcttgacgg cggcgcagaa ggtgctgatg acggttgccg cgccgcacat gtcgaacttc
     4501 atttcgtcca tgttcaggcc gggtttgagg gagatgccgc cggtgtcgaa ggtaatgcct
     4561 ttgccgacca ataccacagg cgcggcttct ttgtcggccg caccaaaata gctcagctcg
     4621 accaaataag ggtcttcgac gctgcctttg gcgaccgacc agaatgaacc catgttttct
     4681 ttaatgtagt ctttttcgat gattttggcg tgtgcgccca gtttttcggc ttcggctttg
     4741 gcggtgcgcg ctaaaaattc gggcgtgcat tcgttgggcg cggcgttacc caaatcgcgg
     4801 cagaggcttt gtccgtaaac ctgtgcttcg gcgacgcgca aggcttcttt gacggcggct
     4861 tcgtgcgcgg tatggaacac ggcagtttca aatttggcgg gcttggcttc ttttttgtag
     4921 cggtcgaaac ggtaggcggc attgccgaac gcaatcgcaa acgcttcggc aacggctgca
     4981 gcctgcgctt cttcaaagac gtgaacgtcc acattgaccg tttcctgatt ttgcgcccat
     5041 ttggcggctt cggcggcggc cttgttcaat gcggcgcggc cggtgctttt cagacagcat
     5101 acggcaacag cctgcaaacc gttgcctgtc gggatttttg tgtcggcaaa attttgacct
     5161 tcttcaagcg aagacaaaag ggcaagggcg gtcggatggt ttaattgcgc ctcttcggtg
     5221 cagacaaata actgtgcgcc tgcctgctgt tcctgcaaga tttcggtttt tgtgctaaat
     5281 tccacgttta ttctcctgat tgagacggtt gtcggtagtt ttcggacggc ctttcgctca
     5341 aaagaccgtc tgaagacggc tggcacgatt gtaccccatt tgaagcaccg tctgaaacct
     5401 tgcgcggaca atccgcctgc gccgaaccgc ttaccgcccc ctgaccgcga ttctatgatt
     5461 tatcaaagaa acctcatcaa agaactctct tttaccgccg tcggcatttt cgtcgtcctc
     5521 ttggcggtat tggtctccac gcaggcaatc aacctgctcg gccgtgccgc cgacgggcgt
     5581 gtcgccatcg atgccgtgtt ggcattggtc ggcttctggg tcatcggtat gacgccgctt
     5641 ttgctggtgt tgaccgcatt tatcagtacg ttgaccgtgt tgacccgcta ctggcgcgac
     5701 agcgaaatgt cggtctggct atcctgcgga ttggcattga aacaatggat acgcccagtg
     5761 atgcagtttg ccgtgccgtt tgccgttttg gttgccgtca tgcagctttg ggtgataccg
     5821 tgggcagagc tacgcagccg cgaatacgct gaaatcctga agcagaagca ggaattgtct
     5881 ttggtggagg caggcgggtt caacagtttg ggcaagcgca acggcagggt ttattttgtc
     5941 gaaaccttcg ataccgaatc cggcatcatg aaaaacctgt tcctgcgcga acaggacaaa
     6001 aacggcggcg acaacatcat cttcgccaaa gaaggtaact tctcgctgaa cgacaacaaa
     6061 cgcacgctcg aattgcgcca cggctaccgt tacagcggca cgcccggacg cgccgactac
     6121 aatcaggttt ccttccaaaa actcaacctg attatcagca ccacgccaaa actcatcgac
     6181 cccgtttccc accgccgtac cattccgacc gcccaactga ttggcagcag caacccgcaa
     6241 catcaggcgg aattgatgtg gcgcatctcg ctgaccgtca gcgtcctcct actctgcctg
     6301 cttgccgtgc cgctttccta tttcaacccg cgcagcggac atacctacaa tatcttgatt
     6361 gccatcggtt tgtttttaat ttaccaaaac gggctgaccc tgctttttga agccgtggaa
     6421 gacggcaaaa tccatttttg gctcggactg ctgcctatgc acatcatcat gttcgtcatc
     6481 gcaatcgtac ttctgcgcgt ccgcagcatg cccagccagc ccttctggca ggcggttggc
     6541 aaaagtctga cattgaaagg cggaaaatga acctgatttc acgttacatc atccgtcaaa
     6601 tggcggttat ggcggtttac gcgctccttg ccttcctcgc tttgtacagc ttttttgaaa
     6661 tcctgtacga aaccggcaac ctcggcaaag gcagttacgg catatgggaa atgctgggct
     6721 acaccgccct caaaatgccc gcccgcgcct acgaactgat gcccctcgcc gtccttatcg
     6781 gcggactggt ctctctcagc cagcttgccg ccggcagcga actggccgtc atcaaagcca
     6841 gcggcatgag caccaaaaag ctgctgttga ttctgtcgca gttcggtttt atttttgcta
     6901 ttgccaccgt cgcgctcggc gaatgggttg cgcccacact gagccaaaaa gccgaaaaca
     6961 tcaaagccgc cgccattaac ggcaaaatca gcaccggcaa taccggcatt tggctcaaag
     7021 aaaaaaacag cattatcaac gtgcgcgaaa tgttgcccga ccataccctg ctgggcatta
     7081 aaatctgggc ccgcaacgat aaaaacgaac tggcagaggc agtggaagcc gattccgccg
     7141 ttttgaacag cgacggcagt tggcagttga aaaacatccg ccgcagcacg cttggcgaag
     7201 acaaagtcga ggtctctatt gcggctgaag aaaactggcc gatttccgtc aaacgcaacc
     7261 tgatggacgt attgctcgtc aaacccgacc aaatgtccgt cggcgaactg accacctaca
     7321 tccgccacct ccaaaacaac agccaaaaca cccgaatcta cgccatcgca tggtggcgca
     7381 aattggttta ccccgccgca gcctgggtga tggcgctcgt cgcctttgcc tttaccccgc
     7441 aaaccacccg ccacggcaat atgggcttaa aactcttcgg cggcatctgt ctcggattgc
     7501 tgttccacct tgccggacgg ctcttcgggt ttaccagcca actctacggc atcccgccct
     7561 tcctcgccgg cgcactgcct accatagcct tcgccttgct cgctgtttgg ctgatacgca
     7621 aacaggaaaa acgttgaacc aatgccgtct gcccccttcg gacggcattg gtttttatta
     7681 tcatattccc gcagacagat agccgttccc tattatagtg gattaacaaa aaccagtacg
     7741 gcgttgcctc gccttgccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
     7801 ttttgttaat ccactataca ttacctgtca taacagttcc atttttgtta aaactagtct
     7861 atgatagcgg tacaaatatt gtttacaata tttaacgcaa atcatttgca acccgacaaa
     7921 agaaaaacag aaaaaggaac aaagagatgt tagaagccta tcgtaaagcc gccgccgagc
     7981 gcgccgccct cggcattccc gccctccctt tgaacgcgca gcaaaccgcc gatttggttg
     8041 agctgctgaa aagcccgccc gcaggcgaag gcgagttctt ggtcgaactg cttgcccacc
     8101 gtgttccgcc cggtgtggac gatgccgcca aagtcaaagc ctcattcctg gctgccgttg
     8161 ccgaaggcag cgcgtccagc ccgctgatct cccccgaata tgcgaccgaa ctcttaggta
     8221 cgatgctcgg cggttacaat atccacgcct taatcgaact cttggacgac gacaaactcg
     8281 cgcccattgc cgccaaaggc ttgaaacata cgcttctgat gttcgattcc ttccacgacg
     8341 ttcaggaaaa agccgaaaaa ggcaacaaat acgcgcaaga agttttgcaa tcttgggcag
     8401 atgccgaatg gttcgcctca cgcgccaaag ttcccgaaaa aattaccgtt accgttttca
     8461 aagttgacgg cgaaaccaat acagacgacc tctcccccgc gcccgacgcg tggagtcgtc
     8521 ccgatattcc gctgcacgcg ctggccatgc tgaaaaaccc gcgcgacggc atcacacccg
     8581 acaaaccgag cgaagtcggt ccgattaaat tgttggaaga actcaaagcc aaaggccatc
     8641 cagttgctta cgtcggcgac gtggtcggta ctggttcttc acgcaaatcc gcgaccaact
     8701 ccgtcatttg gcataccggc gaagacattc cgttcgtgcc gaacaaacgc ttcggcggcg
     8761 tatgtttggg cggcaaaatc gcgccgattt tcttcaatac ccaagaagat tccggcgcgc
     8821 tgccgattga agtcgatgta tctgctctaa aaatgggcga tgtcgtcgat atcctgcctt
     8881 atgaaggcaa aatcgtgaaa aacggcgaga ctgttgccga gtttgaattg aaatcacaag
     8941 tattgctgga cgaagtgcaa gccggcggcc gtatcaacct gattatcggc cgaggtctga
     9001 ccgccaaagc gcgcgaagcc ctgaaactgc ctgcctctac tgcattccgc ctgccgcaag
     9061 cgcctgccga aagcaaagcc ggtttcacct tggcgcaaaa aatggtcggc cgcgcctgcg
     9121 gtctgcccga aggacaaggc gtgcgtccgg gtacttactg cgaaccgcgt atgacgacgg
     9181 tcggctcgca agacacgacc ggcccgatga cccgcgacga gttgaaagac ttggcttgtt
     9241 tgggcttctc cgccgatatg gtgatgcagt ctttctgcca caccgccgcc tatccgaaac
     9301 ctgtcgatgt aaaaacccat aaagaactgc ccgcctttat ttccacccgt ggcggcgtgt
     9361 ccctgcgtcc gggcgacggt gtcatccact cgtggctcaa ccgcctgctg ctgcccgata
     9421 ccgtcggcac cggcggcgac agccataccc gtttccccat cggtatttcc ttccccgccg
     9481 gctccggctt ggttgccttt gccgccgcaa cgggcgtaat gccgctcgat atgcctgagt
     9541 ctgtattggt acgcttcagc ggcaaactgc aaccgggcgt aaccctgcgc gatttggtga
     9601 acgccattcc gctgtacgca atcaaacaag gtttgctgac cgttgccaaa gccggtaaga
     9661 aaaacatctt ctccggccgc atcctcgaaa tcgaaggcct gcctgatttg aaagtggaac
     9721 aagcctttga attgaccgac gcatccgccg aacgctccgc cgccggctgt accgtgaagc
     9781 tcaataaaga gccgattatc gagtacatga aatccaacgt cgtgttgatg aaaaacatga
     9841 ttgccaacgg ctatcaagat ccgcgcactt tggaacgccg catcaaagct atggaaaaat
     9901 ggctggcaaa tcccgagttg ctcgaagcgg ataaagatgc cgaatacgcc gccgtgattg
     9961 aaatcaacat ggacgacatc aaagagccga ttatcgcctg cccgaacgac ccggacgacg
    10021 tgtgcttcat gtccgaacgc tccggcacca aaatcgacga agtattcatc ggttcgtgca
    10081 tgaccaacat cggccacttc cgcgctgctt ccaaactttt ggaaggcaag gcagacaccc
    10141 ccgtccgcct gtggattgcg ccgccgacca aaatggacgc gaaacaattg tccgacgaag
    10201 gacactacgg cgtactcgga cgtgccggcg cgcgtatgga aatgccgggt tgctccttat
    10261 gtatgggtaa tcaggcgcaa gtacgcgaag gtgcgaccgt tatgtccacc tccacccgca
    10321 acttcccgaa ccgtttgggt aaaaacacct ttgtttacct cggttcggcg gaattggcag
    10381 cgatttgctc caaactgggt aaaatcccga ccgttgaaga atatcaagcc aatatcggca
    10441 tcatcaacga acagggcgat aaaatctacc gctatatgaa cttcaacgaa atcgacagct
    10501 acaacgaagt agccgagact gtgaacgttt aatccccgtc atccgtatga agtaagggat
    10561 tgaccgcaat gccgtctgaa caaccttcag acggcattgc aacattccgc taacccttct
    10621 ttccgcaaac gctgcaaata cggcgttcac gcccccacat aaaggaaacg acagtgaacc
    10681 tgaaaaaccg ccattttctg aaacttttag acttcacgcc ggaagaaatc accgcctacc
    10741 tcgaccttgc cgccgaattg aaagccgcca aaaaagcagg gcgcgagatt cagcggatga
    10801 aagggaaaaa catcgccctg atttttgaaa aaacctctac tcggacgcgc tgcgcgtttg
    10861 aagtcgccgc gcgcgatcaa ggcgcgggag tgacttattt agagccgtcc gccagccaaa
    10921 tcgggcataa ggaaagcatc aaagacaccg cccgcgtgtt gggcaggatg tacgatgcca
    10981 tcgaatatcg cggtttcggt caggaagttg ttgaagaatt ggcgaaatac gcgggcgtac
    11041 ccgtgttcaa cgggctgacc aacgagttcc atcccacaca aatgcttgcc gacgcactga
    11101 ctatgcgcga acacagcggc aaacctttga accaaaccgc gtttgcctac gtcggcgacg
    11161 cgcgttacaa catgggcaat tccctgctga ttttaggggc aaaattgggg atggacgtgc
    11221 gtatcggcgc accgcaaagc ctgtggccgt ctgaaggcat tattgccgcc gcacacgccg
    11281 ccgccaaaga aaccggcgca aaaattaccc tgaccgaaaa cgcgcatgaa gccgtgaaga
    11341 atgttgattt tattcatacc gatgtgtggg tcagcatggg cgagccgaaa gaagtctggc
    11401 aggaacgcat cgatttgctg aaagattacc gcgttacgcc cgaactgatg gcggcatcgg
    11461 gcaatccgca agtcaaattc atgcactgcc tgcccgcctt ccacaaccgc gaaaccaaag
    11521 tcggcgaatg gatttacgaa accttcgggc tgaacggtgt ggaagttaca gaagaagtat
    11581 tcgaaagccc cgccagcatc gtgttcgatc aggcggaaaa ccgtatgcac acgattaaag
    11641 cggtaatggt cgcggctttg ggcgactgac agaactgtgc ctgtttaaat tcatccgcaa
    11701 cacagatacc gtctgaacac gatgttcaga cggtatccat atatagtgga ttaaatttaa
    11761 accagtacgg cgttgcctcg ccttgccgta ctatttgtac tgtctgcggc ttcgtcgcct
    11821 tgtcctgatt tttgttaatc cactataaaa aaactgccta caccatgtgt aggcagtccc
    11881 gtttgaaaac aatcagtttt tgtcttggtc aaccaatttg ttggcagtaa tccaaggcat
    11941 catggcacgc agttgtgcgc cgactttttc aacttggtgg tcggcattca gacggcggcg
    12001 ggcagtcata gacgcgtagt tgacattacc ctcttggata aacatttttg cgtattcgcc
    12061 ggtttgaatg cgtttcaggg cattgcgcat ggcttctttg ctggaagcat tgaccacttc
    12121 agggccggta acgtattcgc cgtactccgc attgttggaa atggagtagt tcatattggc
    12181 aataccgcct tcgaaaatca ggtcaacgat cagtttcatt tcgtgcagac attcgaagta
    12241 agccatttca ggcgcgtaac cggcttcggt cagggtttca aaacccgcct tgatcaactc
    12301 gaccacgccg ccgcacaata cggcttgttc gccgaacaga tcggtttcgg tttcttcgcg
    12361 gaaagtggtt tcaatcacac cgcctttggt gccgccgttg gcagccgcat aagacagggc
    12421 gatgtctttg gctttgccgg aattgtcttg gtaaacagca atcagagaag gcacgccgcc
    12481 gccgcgtttg tattcactgc gtacggtatg acccgggcct ttgggggcaa ccataatcac
    12541 gtccaagtcg gcacgcggaa cgatttggtt gtagtgcacg ttgaagccgt gtgcaaatgc
    12601 cagcgttgcg ccttctttca gattggctgc aacttcggcg tgatagacgg caggcatggt
    12661 ttcgtcaggc agcagcagca taacgacatc ggcttctttg gtcgcttcag caacggtttt
    12721 gacgacatgg ccggctgctt cggctttttt ccaagaagaa ccttggcgca gaccgatcac
    12781 cacgtttaca cccgaatctt tcaggttggc agcatgggca tgaccttgcg aaccgtaacc
    12841 gatgatggca acggttttgc ctttgatcag ggacagatcg gcatctttat cgtaatagac
    12901 ttgcatttga tttcctttaa ggtaaatggt tgtcgaagcc ttaaaatgtt gagcggcttc
    12961 ggacgggtta aacagagtgt gccgcttaag cggcaacttc attcatcaat acgatttcca
    13021 acgcttcggt tttgccgtcg acggactgga cgaaggcttg gaaatgcgcg ctggcgttat
    13081 gttcgtcaat agctgcttga gatttccaat tttccacgaa aacaaaacgg ttcggtttgc
    13141 cgatttcctg atggagatcg tagctgatgt tgccctcttc tgcacggctg gctttgacca
    13201 gttctttaaa ctgtgctacc agtgtttccg tgtattccgg tttgacggta accagtgcga
    13261 caattttaat gttcgacata aatctctcct gccgttcgtt tttcagacgg cattcaaata
    13321 ccgtgccgtc tgaaaggtta cggcgttaaa ttttcaaaat acgctcaccg cgaccgatgc
    13381 cggccgcgcc tgtgcgtacg gtttccaaaa tttgggcgcg tccgaccgtt tccaaaaagg
    13441 aatccagctt gtcggtcgag ccggtaattt caatcgtata gctgcggtcg gttacgtcga
    13501 tgatgctgcc ccggtagatt tcggtcaagc gtaaaaattc gtcgcggtct ttgccggcgg
    13561 cacggacttt taccaacatc agttcgcgtt cgacaaaacg gctttcattc aaatcgacca
    13621 ctttaatcac ttcaatcaat ttattgagtt gcttggtgat ttgttcgatg acctgctcgt
    13681 ctccgtgggt aacgatggtc atacgggaca gggttttgtc ttcggtcggc gcaaccgcca
    13741 aagaatcgat attgtaatcg cgtgcagaga acaaaccgac cacgcggctc atcgcacctg
    13801 attcgttttc aatcagaaca gataagatat gtcgcatttg cctctcctta cgccttttcg
    13861 tccgcacgca tatgcggcgg aagtaccatt tcgtccaaac ctttgccgtt gccgaccatg
    13921 ggcatcacat tctgtttctg gtcggtcagg aagtcgataa acaccagcct gtctttttgg
    13981 ttcaatgctt ccaacaacgc accttccaca tcagacttct tgtccacgcg gataccgata
    14041 tggccgtatg cctcggcaag tttgacgaaa tcgggcaaag aatcgaaata ggtttccgac
    14101 tctcgaccgc cgtaatatat ttcctgccac tggcgtacca taccaagata accgttgttc
    14161 agcgtaatga cgttaaccgg aatccgatat tggaaacagg tggacagctc ttggatgttc
    14221 atctgaatcg agccgtcgcc ggtaatacag aatacgtctt gatccggggc ggcaagtttt
    14281 gcaccaatcg cataaggcag acccacgccc atcgtaccca aaccgccgga attgagccat
    14341 tggcgcggac gttcgaaggg ataatattga gccgcaaaca tttgatgctg ccctacatcc
    14401 gatgtgatga ttgccgaatt gccggtaatc tcggcaagct tctgaatcac atattgtggc
    14461 ttgataattt cgctgccgtt gtcaaaccac aagcaatcgc gggaacgcca ttcctctatg
    14521 gttttccacc atttgcccaa agcatcttca gacggcacgg actcttgttt ttgccacagc
    14581 gcaaccatct cggacaaaac gtttttcacg tcgccgacaa tcggaatgtc cgccttcacg
    14641 cgtttggcga tgctggaagg atcgacatcg atatggataa ccttcttcgc cttctcgaaa
    14701 aatttggacg gtacggaaac cacacggtcg tcaaaacgcg cacctacggc aagaacgaca
    14761 tccgcattct gcatggcaag gtttgcctcg taagtaccgt gcataccgag cataccgagg
    14821 aattggcggt cgccggaagg ataagcgccc aagcccatca gcgtacccgt gcacggagca
    14881 cccgtcattc ggacaaatcg ggtcagctct tcagaagcat tacccaacac cacgccgccg
    14941 ccaaaataga cgaccggacg tttggcagat gccaacatct gcacggcctt tttaatctga
    15001 ccgatatgcc cttgaacaac cggttgatac gaacggataa aaatgtcttc ctgaggatag
    15061 ctgaatttcg ccatcgcctg cgtaacatct ttcgggacat caaccaccac gggccccggt
    15121 cggccgcttg cggcaatttg gaacgccttt ttaatggttt ccgccaactc attgatgtcc
    15181 gtaaccagaa aattgtgttt gacgcacgga cgggtaatac ccaccgtatc aacttcttgg
    15241 aacgcatccg taccaatcag ggaattgcct acctgcccgc tgatgaccac catcggaatc
    15301 gaatccgtat aggcagtagc aataccggtc agtgcattgg taacgcccgg gccggatgta
    15361 accaatgcca cgcccacctt accgctgacg cgcgcatacg catctgccgc gtgtactgcc
    15421 gcctgctcgt gacgggtcag aatgtgcttg aatttattga gttggaaaag ggcatcgtag
    15481 atttcgataa ccgcaccgcc gggataaccg aaaacgtact cgacaccttc ggctttgaga
    15541 ctctgcacta tgatttgcgc gcctgatagc tgcataacga cctcttttat acggtttcaa
    15601 accaataggg acaaaccgct ttgccacagc acctgtaatg caattccacc aagcagcgat
    15661 ttagggtacg cgcattgggg gaacacggca acagacggat tatccaatca attggaaagg
    15721 aacacagagt ttgtgaaaaa gagtagaaac gataacgcaa accgacagtt caatcaagaa
    15781 aaatctttca tcttttaata tttttcgaaa gcagagaaat tattgattga ttttaaaaga
    15841 ataaaatcag gagtaccttt tttgaaagat ggaaattgtt gacagtttgt gtaggagggg
    15901 cagatgtgaa aaacccttct tcgatatcaa gaattgtaaa atttacaggg tttcatccca
    15961 ataaagactc gggatattga ttgaacttga ttttattttt gatatatcaa aaatattccc
    16021 aaccatactt cctgaaaatg gctcattgca ccggactgta ttggacggca ttgacagaac
    16081 caagagggct aacaacgact taatatattg
//
LOCUS       DAWFHY010000049        15301 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.049, whole genome shotgun sequence.
ACCESSION   DAWFHY010000049 DAWFHY010000000
VERSION     DAWFHY010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 15301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.049"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(83..3173)
                     /locus_tag="WGQ68_001320"
                     /pseudo
     CDS             complement(83..3173)
                     /locus_tag="WGQ68_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212999.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
     gene            3447..4226
                     /locus_tag="WGQ68_001321"
     CDS             3447..4226
                     /locus_tag="WGQ68_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP reductase"
                     /protein_id="HEZ0715110.1"
                     /translation="MAASPEAKFTEEKILWVKHHTPKLITFAISRPESYRFKAGQFSR
                     LGFYEGEGFIWRAYSIVSAEYADTLEYFAVLIQDGPMSARFAKMQQGDTILLDKNATG
                     FLLPERFPDGKDLVMLCTGSGIAPFLSILEQPEIRQRFDTVNLIHSVSFPEELIFNDR
                     LAALSEHPLVGEYRHSFHFVPVTTRAANPSGLSGKRIPELLKNSSIEQALHTKFTPES
                     TRFMICGNPEMVKDTFQTLLDMGYAMHRNRIPGQIMMENGF"
     gene            complement(4426..5838)
                     /locus_tag="WGQ68_001322"
     CDS             complement(4426..5838)
                     /locus_tag="WGQ68_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0715111.1"
                     /translation="MLFEFKENKMIVASRWPLLEKVFLRFGMPVAVVDLESTGGNLYE
                     DRVTEVALVKFEQGRVVRHEWLVNPQKPIPQFVAGLTGISDGMVADAPVFTEIAGELF
                     SVLKGCVLVAHNSRFDYTFLKHEFHRAGIGFSSPALCSVQLSRCLYPQFYKHSLDSII
                     ERLGIVVEDRHRAMADVSALCDYLEYSLSEHGVEEWIRQCFRLMNPKPLPAALPERLR
                     EQLYGLPDGMGVLACFDGGGKVNYIGTFERVYSEVSALLDSGKAPFDWYNTEEVRFFP
                     ALGSLHAYKIKAELVGRYHSGCYVSAKNLLKTFTTVRFEKGSDGMLNAKTAALKNGVA
                     DNPPTGLFANKKAARRALSSWAETYGLCPASAGILPDGYAEDEPCPVYVSGRCDKACG
                     RSDEQVLAFAHKLPVLDWGKMHEVEITETDPLTGEKVVLHGMGGALEMDGGLWYFDKD
                     LPDVFKAKLKTDRKNIKEIG"
     gene            5929..6005
                     /locus_tag="WGQ68_001323"
     tRNA            5929..6005
                     /locus_tag="WGQ68_001323"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5963..5965,aa:Arg,seq:acg)
     gene            6023..6097
                     /locus_tag="WGQ68_001324"
     tRNA            6023..6097
                     /locus_tag="WGQ68_001324"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6056..6058,aa:Glu,seq:ttc)
     gene            6656..7831
                     /locus_tag="WGQ68_001325"
     CDS             6656..7831
                     /locus_tag="WGQ68_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEZ0715112.1"
                     /translation="MQNIFDPLVIRGKSLTPIVQGGMGVGVSASGLSSAVARENGIGT
                     IASVDLRHLHEDLLAESQINPSEEKYTSLNCTALDREIQKAKSASEGKGLIAVNVMKA
                     VKDHAAYVRQACESGADAVVMGAGLPLDLPEMTEGYHKDVALLPILSESRGINIVLKR
                     WMKKGILPDAIVVEHPAHAAGHLGASTVEGVNDAKFDFKRVIEETFEVFKSLGLESEK
                     IPLILAGGMANFEKVKTALKNWGASAVQIGTAFAVTEEGDAHLNFKKTLAGAETEKVV
                     EFMSVAGLPARGVRTKFLDSYIRREGKLQANAKADPRRCTQGLNCLTSCGLRDGLSKA
                     GQFCIDIQLAAAFRGEVDKGLFFRGKDPLPFGNAIRTVRETIQYLLTGSEPVATLGR"
     gene            complement(8042..8590)
                     /locus_tag="WGQ68_001326"
     CDS             complement(8042..8590)
                     /locus_tag="WGQ68_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HEZ0715113.1"
                     /translation="MSQNTPIKPWYKHVWPWILMAGPIFVVIASVAMFFVAQQHATDL
                     VTDDYYKDGKHIDIQLHRDEEAVRRHIGVQVLISPDMNAAKVFVGGEFDGKQPLNLLL
                     MHPTRKADDQTVALKPVGSAQNGRAEYEAVFKTLPPTNHWYVRVEDAAGVWRVENKWI
                     TSQGNAVDLTPMDKLFNNTESK"
     gene            complement(8593..10089)
                     /gene="ccoG"
                     /locus_tag="WGQ68_001327"
     CDS             complement(8593..10089)
                     /gene="ccoG"
                     /locus_tag="WGQ68_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003708970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HEZ0715114.1"
                     /translation="MTTENQAGSPASGIGTSEQTKAAPKAKKTFDPRASVIQIHPEGE
                     RIHPKKAEGRFAKLRIAAVLATQFVFYVIPWFNWSGRQAVVFNIPERHFFIFGLSLGV
                     GDLIYLALLLMICAFGLFWWTTIAGRLWCGYSCPQTVYTEIMLWIDNLVEGDRNKRLK
                     LEKSPWNFTKIRIKATKYLLIFLVCAWTGITFAGWFVPIRQFVPDLFTGAAGGGAMFA
                     TAFYGFMTFFFAHIMREKVCLHMCPYARFQSAMFDKDTLIVSYDAERGEPRGARKKTV
                     NKEEAGLGDCINCAMCVQVCPVGIDIRNGLQYQCIGCAACIDACDEIMDKMGYPRGLI
                     RYTTESALEHEYSEKDIKKRLLRPRVAGYGAVLALVIIAFLAGLSTRKMVEVDILKDR
                     GVLVRENAKGWLENAYSLRIINKSEKEQLITASVKGFDEIALTGLPEGGIKVAPRETV
                     TLPVQVSTIPEYADKGSHPIEFTFQYRESGAPDGKPVVLEEDATFIGE"
     gene            10618..12597
                     /gene="tkt"
                     /locus_tag="WGQ68_001328"
     CDS             10618..12597
                     /gene="tkt"
                     /locus_tag="WGQ68_001328"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEZ0715115.1"
                     /translation="MSQLANAIRFLSADAVQKANSGHPGAPMGMAEMAETLWTKFLNH
                     NPANPKFYNRDRFVLSNGHASMLLYSLLHLTGYNLSIEDLKNFRQLHSKTPGHPEYGY
                     TDGVETTTGPLGQGIANAVGMALAEKILAAEFNKDGLNIVDHYTYVFMGDGCLMEGVS
                     HEACSLAGTLGLGKLIVLYDDNNISIDGKVDGWFTENIPQRFESYGWHVVPNVNGHDT
                     AAIQAAIEAARAETGKPSIICCKTLIGKGSANKEGSHKTHGAPLGADEIEATRKHLGW
                     TYPAFEIPQEIYDAWNAKEQGAKLEADWNELFAQYQAKYPAEAAEFVRRMDKKLPENF
                     DEYVQTALKEVCAKAETIATRKASQNSIEILAKELPELVGGSADLTPSNLTDWSNSVS
                     VTRDKGGNYIHYGVREFGMGAIMNGLALHGGVKPFGATFLMFSEYERNALRMAALMKI
                     NPVFVFTHDSIGLGEDGPTHQPIEQTATLRLIPNMDVWRPCDTAESLVAWAEAVKAAD
                     HPSCLIFSRQNLKFQARSEQQLNDIKRGGYIISEAQGNAQAVIIATGSEVELALEAQK
                     ALAAQNIAVRVVSMPSTNVFDRQDAAYQATVLPEGLPRVAVEAGHADGWYKYVGLNGA
                     VVGINRFGESAPANLLFKAFGFTVDNVVDTVKSVL"
     gene            12738..14126
                     /gene="fumC"
                     /locus_tag="WGQ68_001329"
     CDS             12738..14126
                     /gene="fumC"
                     /locus_tag="WGQ68_001329"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HEZ0715116.1"
                     /translation="MNTRTEHDTMGNVEVPSEAYWGAQTQRSRNNFKIGGETLPQPLI
                     YALALVKKAAAATNVSLGRIKPEQADLITQAADDVLNGKLNGQFPLVVWQTGSGTQSN
                     MNMNEVLANRANEIAGTGLAAYQPVHPNDHVNHAQSTNDAFPTAIHVAAAIEINRHLI
                     PAVKALRDTLDKKAQAFAPIVKIGRTHLQDATPLTLGQEFSGYVSQLDHGLGRLNDAL
                     KDLYELALGGTAVGTGLNSHPEYAEKAAAKLAELSGLPFVSAPNKFEALGGRDAAVAA
                     SGALKTLAASLNKIANDIRWLASGPRCGLGEIKIPENEPGSSIMPGKVNPTQCEAMTM
                     VCCQVFGNDVTIGMAGASGNFELNVYMPVIAYNLLQSIHLLGDACNSFNEHCAIGIEP
                     VPEKIDYFLHHSLMLVTALNRKIGYENAAKVAKTAYKNNKSLRETAVELGLLTGEEFD
                     ELVVPADMVHPR"
     gene            complement(14191..15114)
                     /locus_tag="WGQ68_001330"
     CDS             complement(14191..15114)
                     /locus_tag="WGQ68_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0715117.1"
                     /translation="MENQRPLLGFALALLAAMTWGTLPIAVRQVLKFVDAPTLVWVRF
                     TVAAAVLFVLLALGGRLPKRRDFSWCSFRLLLLGVAGISANFVLIAQGLHYISPTTTQ
                     VLWQISPFTMIVVGVLVFKDRMTAAQKIGLVLLLAGLLMFFNDKFGELSGLGAYAKGV
                     LLCAAGSMAWVCYAVAQKLLSAQFGPQQILLLIYAASAAVFLPFAEPAHIGSLDGTLA
                     WVCFAYCCLNTLIGYGSFGEALKHWEASKVSAVTTLLPVFTVIFSLLGHYVMPDTFAA
                     PDMNGLGYAGALIVVGGAVTAAVGDRLFKRR"
BASE COUNT         3854 a         4380 c         3525 g         3542 t
ORIGIN      
        1 gtcccgatag acctgcctcc ataaaaatgc cgtctgaaac ataccctgtt tcagacggca
       61 tccgcaaaac tacagttttc aattaaaact gccaatccag tttcatgctg acagtgcgcg
      121 gctctccata gaagttgttt gcgccgcgcg tacggttgta gttgttctca aaataagtgc
      181 gtccgtttaa gttcgtaccg atgaggctca atttggcgtg tttgcccaat tcgtaacgga
      241 cgaaaccgtc tatcagcccg tagccgccct gcctgatgtt atacagactg cttgtgccgc
      301 tttgtgcgga cacgccgccg ccgacggtca gccccgtatt cggtatatgg aagctcgttc
      361 cgaaacggaa tatgtgcacg ggtgtgaaat tgctgaagtt gtacgggtct gcgcctgtgt
      421 ttttggcgag gcgttcggcg ttgacttcgg cggcgttttt gtagcggctc ttgttgtagg
      481 tgtaacccgc aaagactttc caatcttcgt tcaactcacc cgacaactcg aattccgcac
      541 ccctgctgac cactttgcct atcggtttgg caacggtttg gaacgacccc tgcttgccgc
      601 ctgctccggg aacatagcca aaatcgacga ccgtgcggtt tttctgttcg aggtaaaaca
      661 atgcgaacga agcattcagc cgtccttgca agaacgcgcc tttccagcct acctcatagt
      721 ttgtgccgac caaaggcggt aaaacggttt tggcactgac atcgacatta tcctgctgtt
      781 tgaagatttt ggtataactt ccgtaaatac tctgttgcgg tgtcaaatca taggtaatgc
      841 ctgcataggg cgtcaattta tgaccttgca tcttggccgt ataatggtcc tgatccgcct
      901 taatgctcga tgccgtctga aaatcgcttg ccggctgccc atagcggaca ggcatatctt
      961 tggtttgcga ggtttcatag cgcgtgtagt gcagcccgcc caaaagatgc agtcggccgg
     1021 ttacgttgaa acgcgtgccg gcagtcagcg aatgggtttt gttggtgttg aggtatttgg
     1081 cgtagttata cagcgcagga acatgatcgt cggccacttt gacggttttc caaaccggca
     1141 ccgtaccgga aaaaccggta aaggcaggcg agccgtcggg attggtctcc tgaatcttgt
     1201 tgcctttttc gtccagctca tatacatcga catataccgg tgtccggctg ccgctgtatt
     1261 cgtcatagta atatacctgt ttgccttcgg catcgagctt gggctcggtt tttattttct
     1321 tggcgttcct gcattcttcg gcataaacgg tacggttgcc tttttcatcg tacgccttcc
     1381 aatcgggttc tttatgcccc ctgaccaaag gagacgacaa atcgccgtcc ggctcctcct
     1441 gacaacttcc cgcatacacg ccgtgcgttg cccccgtatt cggacgtact ctgtggcggc
     1501 gttcgtagat ttccagatat tcggaacgta tcttttcatc gccgtaggca tagccgacaa
     1561 agaaatcgtg ttcccgcccg aacagcccat atgtgccggt caggtcaagt ttgattcccc
     1621 attggcggtc gtctttggta tgccgcaacg gcatataact gtatcgtcgg ttggcggtag
     1681 ccttacgatt aaaggaagag tcatacaggc tgtttggaaa acgttgcgcc gcattattaa
     1741 agatgccctc cttcgcaagg gctttatcga caaattccgc cttgtcggca tcaacgcccg
     1801 gatccccccc aagaaccttg acagataaag tccagcgcaa aaggggcact catacactcg
     1861 tcaaaaccgg ctttgcgttc tgcggcacgg cggctgcgat actgttcgaa agcagtatta
     1921 tcgaaacggt ttttaacaaa atcgtctttg cgctcccggt attccttggc ggtttcatca
     1981 cgatatgctt tcagtttctc caatgcctta tctttcggct cgaacgggat gacttcgttt
     2041 ttttcagtca aaaagcctac cgcatcctca cccgacaaac ccgccgcatg ttcgtttttc
     2101 agaaaaaact gccccacctt cgcatcggat tcattcttgg tataagacac ttcggcattg
     2161 agctgccaac cgttgtcaaa cacatgtttg aaacctgaga aaaggttgta tttgtcagca
     2221 ctcaaccgcg accaatcctc ccccaaataa gtgttgcgcg gcagttgcaa aggccggttg
     2281 caggcaggcg ttgaactgaa cggggcagtt ttctgatttt cacaggacaa aataatgccc
     2341 gaaaaatcag gaacctccct actcttctga tacatgccgc ccaaagtaag cacactgctg
     2401 tcgcccgcat cggcttcggc aatgccgtaa accatatgtt tcctgcccca aacgcggtct
     2461 ttaaacgatt ttttatactc ttccgcaccc accaaccttc cgcgtaaggt attcgcctta
     2521 ttcaggctgc ctgaaacatc caacactgca cgccggctgc cgcgatggtc ggcggtcagc
     2581 tctccggtat gtttgaaaga agcggtaggt cgcttacgga tcaaattgac ggttccaccc
     2641 ggctctgaat tggattgggt caaccccgtt gcaccccgta caacttcaat atggtcataa
     2701 accgccaaat cggtactcgg agacacgtcg attttcgccg tatatcccga acggcctgca
     2761 acattgacgg taataccgtc ttcaccaatc tgatcaatat agaaaccgcg tgacaaaaac
     2821 cgcgtctgca agcctgaatc gcgcacaacg ttgacacccg tcgtgttttt cattgcctct
     2881 tcaagcgtat gcaccgcctt atcgtcaagg cggctgcgcg tgatgacgct gaccgactgc
     2941 ggcgtatcct tgcccgcaat cctcatacct gtggcggtgg acatccgatc tatcgtataa
     3001 gaacgggtct tctcggtctt gcccgacaga gcatgagagc cgcgtacatt gaccgtatcc
     3061 agactgacgg tattgccgtc tgaaacaggc acaacaccgt ctgcaaaaga accaccgtaa
     3121 gccgataaca gcataacggt cagaatttta agtgaaaaat gattttgatt catagagacc
     3181 tctgtaatat gcaagtgtgc aaatcgtcca aaggccctca caactgtttt gattttttat
     3241 attaattgaa aaaaagtaat tctcaattaa atttatagat gggattgtat tcccattttg
     3301 acaaaaaaca aactgctcct taccgtttat ttcaaaaaac gataatattg tattgaaaaa
     3361 tatccgaatt taaatacaga tcgtcaatgc ggaaaaaaac acccaaattg gctataatcc
     3421 cgacaaacac actcaaggac aacaacatgg cagcctcgcc cgaagcaaaa ttcactgaag
     3481 aaaagatttt gtgggtcaaa caccacacgc cgaaactcat cactttcgcc atcagccgtc
     3541 ccgaatccta ccgttttaaa gccggacagt tctcccgact cggtttctac gaaggggaag
     3601 gctttatttg gcgtgcctac tccatcgttt ccgcagaata tgccgacacg ctcgaatatt
     3661 ttgccgtact catccaagac ggccccatgt cggcccgttt cgccaaaatg caacagggcg
     3721 acaccatcct gctcgataaa aatgccaccg gcttcctcct gcccgaacgc ttccccgacg
     3781 gcaaggattt ggtgatgctc tgcaccggct ccggcatcgc ccccttcctt tccattctcg
     3841 aacaacccga aatccggcag cgtttcgata ccgtcaacct gatacattcc gtatcttttc
     3901 ccgaagaatt gattttcaac gaccggctcg ccgcattgtc cgaacatccc ctggtaggcg
     3961 aatacagaca ctcgttccat ttcgtccctg ttaccacccg tgccgccaac ccctcgggct
     4021 taagcggaaa acgcattccg gaactcttaa aaaacagcag catcgaacag gcgctgcata
     4081 ccaagtttac cccggaatcc acacggttta tgatttgcgg caaccccgaa atggtcaaag
     4141 acactttcca aacgctgctc gacatgggtt acgccatgca ccgcaaccgc attcccggtc
     4201 aaatcatgat ggaaaacggc ttctaaaaac caccctgctt gtccgatgcc tcggacgggc
     4261 aaaccggcac gacacgcaaa ccgcgtcggc aaaaatgccg tctgaagcct tcagacggca
     4321 ttttcggata catacaaacg gcaacatatt ccaacccaaa ccgattaggc aatcaatacg
     4381 aacggctgtt tacatactta ccggctttca ccaaccgata tcgatttaac cgatttcctt
     4441 aatatttttc ctgtccgttt taagcttcgc cttaaacaca tccggcaaat ctttatcgaa
     4501 ataccaaagc ccgccatcca tttccaatgc gccgcccatt ccatgcagaa caactttttc
     4561 ccccgttaaa ggatcggttt cggtaatttc cacctcatgc atttttcccc aatccaaaac
     4621 aggcaacttg tgtgcaaacg ccaaaacctg ttcgtcagaa cggccgcacg ccttatcgca
     4681 tcggcctgaa acatatacgg gacaaggctc atcctctgca taaccatccg gaaggatacc
     4741 ggctgaggca ggacacaatc cgtatgtttc cgcccacgac gacaaagccc gtctcgccgc
     4801 ctttttattg gcaaataatc cggtaggcgg attatccgcc acaccgtttt tcaaagccgc
     4861 tgttttcgca ttcaacatgc cgtctgaacc tttttcaaac ctgacggtcg taaatgtttt
     4921 aagcagattt ttggcagata cataacaacc cgaatgataa cgcccgacca attccgcttt
     4981 aatcttatat gcatgcaggc tgcccaatgc aggaaaaaaa cggacttcct ccgtattgta
     5041 ccaatcaaac ggggctttcc cggaatccaa taaagccgaa acctcgctat atacccgttc
     5101 aaacgtaccg atgtaattta ctttccctcc gccgtcgaaa caggccagca cccccatacc
     5161 gtcaggcaaa ccgtacaact gttccctcaa ccgttcgggc agcgcggcag gcagcggttt
     5221 cggattcatc aaacggaaac actgcctaat ccactcctca accccgtgtt ccgacagact
     5281 gtattccaaa taatcacaca atgccgacac atccgccatc gcacgatgcc tgtcttccac
     5341 aacaatcccc aacctttcga tgatactgtc caggctgtgc ttgtaaaatt gcggatacag
     5401 acaccgggac agttgcacac tgcacaaagc aggcgatgaa aatccgatac ccgcacgatg
     5461 aaactcatgc tttaaaaacg tatagtcgaa acggctgtta tgtgcaacca gcacacaacc
     5521 cttcaatacc gaaaacaact cgccggcaat ctctgtaaaa acaggtgcgt cggcaaccat
     5581 gccgtctgaa atccccgtca gccccgccac aaactgcgga atcggttttt gaggattaac
     5641 caaccactca tgcctcacca cccttccctg ctcaaacttg accaaagcca cttcggttac
     5701 cctgtcttca tacagattgc cgcccgtcga ttccaaatcg accacggcaa ccggcattcc
     5761 aaaccgtaaa aatacctttt ccagcaaggg ccagcgggaa gcaacaatca ttttattctc
     5821 tttaaattca aacaacaaac cgatatttta cacttttaag gcatttcatc caacaaaaca
     5881 attgacagaa tccgatgatt accctaaaat tcaaatcttt cttgcagcgc acccgtagct
     5941 cagttggata gagtatctgg ctacgaacca gagggtcggg cgttcgaatc gctccgggtg
     6001 cgccagtaag aaaatacaat atgcgcccat cgtctagcgg ttaggacatc gccctttcac
     6061 ggcggtaacc ggggttcgat tccccgtggg cgtgccaaat tctaaatccc cgagattatc
     6121 gctcggggat tttttattgt ctcagcaact cgttaccata tctttaccta cccccttcat
     6181 cagaatctca gacgtaatcg aatcatattc aaacctttgc cgtgcaaacc gatatcccat
     6241 aaccggatgc ggtgtccgtc caacatttta cccgattgaa acgcctgata tattgcaccc
     6301 catcaacgtg gcattacttt tcttaacaat cccctttgac agcaactgac tagggctttt
     6361 ttatgccatc atcaaattta tagtggatta actttaaacc agtacggcgt tgcctcgcct
     6421 tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt cctgattttt gttaattcac
     6481 tataatattt tctctcccga ttgaaacagg cgtaacagaa tatgcccgaa gctccggctg
     6541 ctttcttgtt taccgccgcg atatttagag tataatacca aatttgagca atagttctaa
     6601 aacagttaga accatttttc atgagcctga ctgattcgta cactcggaga aactgatgca
     6661 gaatattttt gaccctttgg ttattcgtgg aaaatccctt acccccatcg tgcaaggcgg
     6721 tatgggggtc ggtgtttccg catcgggttt atccagcgcg gtggcgcgtg aaaacggtat
     6781 cggaacgatt gccagtgtgg atttgcgcca ccttcacgaa gacctactcg ccgaatcaca
     6841 aatcaatccg agtgaagaga aatatacatc tttgaactgt accgcattag acagggaaat
     6901 ccaaaaagcc aaaagcgctt cagagggaaa aggactgatt gcggtcaacg tgatgaaggc
     6961 agtcaaagac cacgccgcgt atgtccgcca ggcttgcgaa tcaggggcgg atgcggtcgt
     7021 gatgggcgca ggcctgcctt tggacctgcc ggaaatgacc gagggctatc ataaagatgt
     7081 cgcgctgctg ccgattctgt ccgaatcgcg cggtattaat atcgtcttga aacgttggat
     7141 gaaaaaaggc atattgcccg atgcgattgt agtcgaacat cctgcccacg cggccgggca
     7201 tttgggcgcg tcaaccgtcg aaggcgtaaa cgatgctaaa ttcgacttca aacgcgtgat
     7261 tgaggaaacg tttgaagttt tcaaaagttt agggctggaa agcgaaaaaa tcccgcttat
     7321 tttggcaggc ggtatggcaa actttgaaaa agtcaaaacc gccctgaaaa actggggagc
     7381 atccgccgtt caaatcggta cggcttttgc cgttaccgaa gaaggagatg cgcaccttaa
     7441 cttcaaaaaa acgcttgctg gtgcggaaac tgaaaaagta gtcgaattta tgtctgttgc
     7501 cggtttgccg gcgcgcggcg tacgtaccaa attcctagac agctatatca ggcgcgaagg
     7561 caaacttcag gcaaacgcca aagccgaccc gcgccgctgc acccaaggat taaactgcct
     7621 gaccagctgc ggcctgcgcg acgggctttc caaagcaggg caattctgta ttgatatcca
     7681 gcttgctgcc gcattccgtg gagaagtgga taaaggcctg ttcttcagag gtaaagaccc
     7741 tcttcctttc ggcaatgcca tccgcaccgt ccgcgagacg atacaatatc tgctgacggg
     7801 gagcgaacct gttgcaacgc tcggacgctg acatcaggta ggatttgatt tgcaaacaaa
     7861 atgccgtctg aagggccttc agacggcgtt tttcaggctg cgttctggaa tagtgttaaa
     7921 aaataaacgg gatgagctgc atttattttc gtccgacaaa tcaaaccatc acgccgaatg
     7981 atcaaataat gcctgcacgg cgttgcatct ggcaaatcaa tgcaatgaaa acacggcttt
     8041 tttatttgct ttcagtatta ttgaaaagtt tgtccatcgg ggtcaaatcg accgcattgc
     8101 cttggctggt aatccatttg ttctcgacac gccacacgcc tgccgcgtcc tccactcgca
     8161 cataccagtg gttggtcgga ggaagggttt tgaacaccgc ctcatattcc gccctgccgt
     8221 tctgcgcgct gccgacgggc ttgagggcga cagtttggtc gtccgccttg cgggtcgggt
     8281 gcatcagcag caggttcaaa ggctgtttgc cgtcaaactc gccgccgaca aacacttttg
     8341 ccgcattcat atcgggggaa atgagaacct gcaccccgat atgccgtctg acggcttctt
     8401 catcccgatg aagctggatg tcgatatgct tgccgtcttt ataataatcg tccgtaacca
     8461 aatctgtcgc gtgctgctgc gcgacaaaaa acatagcgac gctggcgatg acgacaaaaa
     8521 tcggccccgc catcaagatc cacggccaga cgtgtttgta ccaaggtttg attggagtgt
     8581 tttgagacac ggttattctc cgataaaggt tgcatcttct tccaagacga ccggcttgcc
     8641 gtcgggcgcg ccgctttcgc ggtattggaa ggtaaattcg atagggtggc tgcctttgtc
     8701 cgcgtattcc ggaatggtgg acacttggac gggaagggtt accgtttcgc gcggggcaac
     8761 cttgataccg ccttcgggca gcccggtcag ggcgatttcg tcaaagcctt tgacacttgc
     8821 ggtaatgagc tgttcttttt cacttttgtt gatgatacgc aggctgtatg cgttttccag
     8881 ccagcctttg gcgttttcgc gcaccagtac gccacggtct ttcaaaatat cgacctcgac
     8941 cattttgcgc gtggacaaac cggccaggaa ggcaatgata actaacgcca acaccgcgcc
     9001 gtaacctgcc acgcgcggtc tgagcagccg ttttttaatg tctttttcag aatattcgtg
     9061 ttccaacgcg ctttcggtcg tataacggat taatccgcgc ggatagccca ttttgtccat
     9121 aatctcatca cacgcgtcga tacaggcggc gcagccgata cattggtact gcagaccgtt
     9181 gcggatgtcg atgccgacag ggcagacttg gacgcacatc gcacagttga tgcagtcgcc
     9241 caaacccgcc tcttccttat tgaccgtttt cttgcgcgcg ccgcgcggtt cgccgcgttc
     9301 cgcgtcataa gaaacaatca gcgtgtcctt gtcgaacatc gcgctttgga aacgtgcata
     9361 cggacacata tgcaggcata ctttttcgcg cataatgtgg gcgaagaaga aggtcataaa
     9421 gccgtaaaac gctgtggcaa acatcgcgcc gccgcctgct gctccagtga ataaatcggg
     9481 aacgaactgg cggataggga caaaccagcc tgcaaacgtg atgcccgtcc acgcgcagac
     9541 aaggaaaatc agcaggtatt tggtggcttt gatgcggatt ttagtgaaat tccacggcga
     9601 tttttccagt ttcagccgtt tgtttctatc gccttcgacc aggttgtcga tccacagcat
     9661 aatttcggtg taaaccgttt gcgggcagga atagccgcac cacagtcgcc cggcaatcgt
     9721 cgtccaccaa aacagcccga aggcgcaaat catcagcagc aaggcaaggt aaatcaaatc
     9781 gcccaccccc aacgacaatc cgaaaatgaa gaaatgccgt tcggggatat tgaaaacgac
     9841 ggcctgccta ccgctccagt tgaaccacgg aatgacgtaa aacacaaact gcgtcgccaa
     9901 tacggcggcg atacgcagtt tggcgaaccg tccttccgcc tttttgggat ggatgcgttc
     9961 gccttcggga tggatttgaa tcacgctggc tcgtggatcg aatgtttttt ttgctttcgg
    10021 cgcggctttt gtttgttcgg acgtgccgat tccggatgcc ggactgccgg cttggttttc
    10081 cgtggtcatt ctgcattcct tagatttttg attgatggtt tgctcgttac cgccgccgtt
    10141 tgcttttcag acgtcatttt tcttgttttt taaggcgttg tgtttcaagt tttgagaaaa
    10201 tccgtttttc ccaaaatata tttccgctat tgtacaactt tatgcgccgt ccggatgtat
    10261 ggggcggata catttcccat ccgcatcaaa acgcctggat tttaccttac cgcccgaaca
    10321 aaatccgaat acggttaaaa aaaaagacta aaaaaaaccg acacccccat atcggcagaa
    10381 ccgacggcgc aagctcataa acaaacgcta tcgacaatcc ggcacacaat ctataacttt
    10441 ttatttcaaa aggaataatg gcaggcttcg cccgcaaatc gaaaatcctt ccccaccggt
    10501 ctcctgccgc cgccttcccc cgcgtccgcc cttttcttga aagcataagc gaatcgggcg
    10561 ataatcaacg ctttccgatt atccacttat ccaaaacacc agcaaggaaa atacaaaatg
    10621 tctcaactgg caaacgcaat ccgcttcctc tcggccgatg ccgttcaaaa agccaattcc
    10681 ggccaccccg gcgcgcctat gggtatggcg gaaatggcgg aaacattgtg gacgaaattc
    10741 ctcaatcaca accccgccaa ccccaaattc tacaaccgcg accgcttcgt cctctccaac
    10801 ggccacgcgt ctatgctgtt gtacagcctg ctgcacctga ccggctacaa tctgagcatt
    10861 gaagacttga aaaacttccg ccaactgcac agcaaaaccc ccggccatcc cgaatacggc
    10921 tataccgacg gcgtggaaac cacgaccggc ccgttggggc aagggattgc caacgcggtg
    10981 ggtatggcat tggcagaaaa aatccttgcc gccgaattta ataaagacgg tttgaacatc
    11041 gtcgatcatt acacctacgt ctttatgggc gacggctgtc tgatggaagg cgtatcgcac
    11101 gaagcctgtt cgctcgccgg caccttgggc ttgggcaaac tgattgtttt gtatgatgac
    11161 aacaatattt ccattgatgg taaagtggac ggctggttta ccgaaaacat cccgcaacgc
    11221 tttgaaagct acggctggca cgtcgttccc aatgtaaacg gtcatgacac cgccgccatt
    11281 caagccgcca tcgaagccgc acgtgccgaa accggcaaac cgtccatcat ctgctgcaaa
    11341 accttaatcg gcaaaggcag tgccaacaaa gaaggcagcc acaaaaccca cggcgcacct
    11401 ttgggcgcgg acgaaatcga agccacgcgc aaacatttgg gctggactta ccccgccttt
    11461 gaaatcccgc aagaaattta cgatgcgtgg aatgccaaag aacaaggcgc gaaactggaa
    11521 gccgactgga acgaactgtt cgcgcaatat caagccaaat atcctgccga agccgcagaa
    11581 tttgtgcgcc gtatggataa aaaactgccg gaaaactttg acgagtacgt tcagacggca
    11641 ttgaaagaag tgtgcgccaa agccgaaacc atcgctaccc gcaaagccag ccaaaacagc
    11701 atcgaaatct tggcaaaaga gctgcccgaa ctggtgggcg gttcggccga cctgaccccg
    11761 tccaacctga ccgactggtc aaacagcgtc tccgttaccc gcgacaaagg cggcaactac
    11821 atccactacg gcgtgcgcga gttcggtatg ggtgcgatta tgaacggtct tgccctgcac
    11881 ggcggcgtaa aaccctttgg cgcgaccttc ctgatgttta gcgaatacga acgcaacgcc
    11941 ttgcgcatgg cggcactgat gaaaatcaac cctgtatttg tgttcaccca cgattccatc
    12001 ggtttgggcg aagacggccc gacccaccag ccgattgagc aaaccgccac cctgcgcctg
    12061 attccgaaca tggacgtatg gcgtccgtgc gacaccgccg aatctctggt ggcttgggca
    12121 gaagccgtca aagccgccga tcatccgtcc tgcctgattt tcagccgtca aaacctgaaa
    12181 ttccaagcgc gcagcgaaca acaactgaac gacatcaaac gcggcggcta catcatcagc
    12241 gaagcccaag gcaacgcgca agccgtcatc attgccaccg gttccgaagt cgagctggct
    12301 ttggaagcgc aaaaagccct cgccgcgcaa aacatcgccg tacgcgtcgt ttccatgccg
    12361 tccaccaacg tattcgaccg ccaagacgcc gcctatcaag ccaccgtcct gcccgaaggt
    12421 ctgccgcgcg ttgccgtaga agccggacac gccgacggct ggtacaaata cgtcggtctg
    12481 aacggcgcag tcgtcggcat caaccgcttc ggcgaatccg cccctgccaa cttactcttc
    12541 aaagcattcg gctttaccgt ggacaatgtg gtcgatacag tgaaatcagt gctgtaaccc
    12601 cactcctaaa caaatgccgt ctgaacgctt ttcagacggc atttttttgc cctcccgcct
    12661 tgatgcttcc tcattccacc aatctccatt ataatatttg cgaatcactc ttattcacat
    12721 ttcaaaagga gaaacgtatg aacactcgca ccgaacacga caccatgggc aatgtcgaag
    12781 tcccgtccga agcctattgg ggcgcgcaga cccagcgcag ccgcaacaat ttcaaaatcg
    12841 gcggcgaaac cctgccgcag ccgctgattt atgctttggc gttggtgaaa aaagccgctg
    12901 ccgcgaccaa tgtttccctc ggcaggatta agccggaaca ggcggatttg attacgcagg
    12961 cggcggatga tgtgttgaac ggcaagctca acggacagtt tcctttagtg gtttggcaga
    13021 ccggctccgg cacgcagtcc aatatgaaca tgaacgaagt gctggcgaac cgcgccaacg
    13081 aaatcgccgg tacgggtttg gcggcgtatc agcccgtcca tcccaacgac catgtgaacc
    13141 acgcgcaatc gaccaacgac gcattcccga ccgctatcca cgttgccgcc gcgattgaaa
    13201 tcaaccgcca cctcatcccc gccgtaaaag ccctgcgcga caccttggac aaaaaagccc
    13261 aagccttcgc ccctatcgtc aaaatcggcc gcacccactt gcaggacgcg acgccgctga
    13321 ctttaggcca agaattttcc ggctacgttt cccagcttga tcacggctta ggccgtctga
    13381 acgatgcgct taaagacttg tatgaacttg ctttgggcgg tacggcggtc ggcacgggtt
    13441 tgaacagcca tcccgaatac gccgaaaaag ccgccgccaa actcgccgaa ctgtccggct
    13501 tgccgtttgt cagcgcgccg aacaaatttg aagccttagg cggacgcgat gccgccgttg
    13561 ccgcttcggg cgcattgaaa acgctggcgg caagcctgaa caaaatcgcc aacgacatcc
    13621 gttggctggc aagcggcccg cgttgcggtt tgggcgaaat caaaatcccc gaaaacgagc
    13681 cgggttcgtc cattatgccg ggcaaagtca acccgaccca atgcgaagcg atgaccatgg
    13741 tgtgctgcca agtgttcggc aacgacgtta ccatcggtat ggcgggcgcg tcgggcaatt
    13801 tcgagctgaa cgtctatatg cccgtcatcg cctacaatct cttgcaatcc atccacctgt
    13861 tgggcgacgc gtgcaacagc ttcaacgaac actgcgccat cggcatcgaa cccgtgccgg
    13921 aaaaaatcga ctatttcctg caccattccc tgatgctggt taccgcatta aaccgtaaaa
    13981 tcggttacga aaacgccgcc aaagtcgcca aaaccgccta caaaaacaac aaatcgttgc
    14041 gcgaaaccgc cgttgagttg ggcttgctga cgggcgaaga atttgacgaa ctggtcgttc
    14101 ctgccgatat ggttcatccg cgctaatcct tccctcaaat aaaatgccgt ctgaaacctc
    14161 gttcggacgg cattttccgt tgcctgcaaa ctagcggcgt ttgaacagcc tgtcccccac
    14221 cgccgccgta accgcacccc cgaccacgat cagtgcgcct gcataaccca aaccgttcat
    14281 atccggcgcg gcaaaagtat caggcatcac ataatgcccg agcaaagaaa atattacggt
    14341 aaacacgggg agcaaggttg ttaccgcgct gactttggaa gcctcccaat gtttcaacgc
    14401 ctcgccgaac gagccgtaac cgattaacgt attcaagcag caatacgcaa aacaaaccca
    14461 cgccaacgta ccgtccaaac ttccgatgtg tgccggttcg gcaaacggca ggaacacggc
    14521 ggcacttgcc gcataaatca acagcagaat ctgttgcggc ccgaattgcg ccgacagcag
    14581 cttttgcgcc acggcataac acacccatgc catactgcct gccgcacaca gcaacacgcc
    14641 cttcgcatac gcgcccaaac ccgacaactc gccgaattta tcgttaaaaa acataagcaa
    14701 accggcaagc agcaaaacca agccgatttt ctgagcggca gtcatccggt ctttaaacac
    14761 caacacaccg acaacaatca tcgtaaacgg cgaaatctgc cacaaaacct gcgtcgtggt
    14821 cggcgaaata taatgcagcc cttgggcaat cagcacaaag tttgccgaaa tgcccgccac
    14881 gccgagcagc agcagcctga atgagcacca agaaaaatcc cgccgcttcg gcagccgccc
    14941 gcccagtgcc agcaaaacaa acaataccgc cgccgccacg gtaaaacgca cccacaccag
    15001 cgtcggcgca tcgacaaact tcaatacctg ccgcacggca atcggcagcg ttccccacgt
    15061 catcgccgcc aaaagtgcca acgcgaagcc taggagcggc ctttggtttt ccatcctgat
    15121 tttcctattt ttaaacaacc gtattgccgg acgatgccgg tttgccgcat cgggcaatga
    15181 tggttcaagc gtttggcgtt tgattccaac cctttgattt caaacaaacc agctgaagct
    15241 cggctattgc ttcgcgctat ttgaaaacac cgcctgaatt ttaaaatata gtggattaac
    15301 a
//
LOCUS       DAWFHY010000050        14966 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.050, whole genome shotgun sequence.
ACCESSION   DAWFHY010000050 DAWFHY010000000
VERSION     DAWFHY010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14966
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.050"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..220)
                     /locus_tag="WGQ68_001331"
     CDS             complement(<1..220)
                     /locus_tag="WGQ68_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium:proton antiporter"
                     /protein_id="HEZ0715118.1"
                     /translation="MDWMGSLFLPGGALLFLSVVSTTLSACLGMPLLLVSPAGVLDGA
                     AEALAIAAFLMLVARPPAVFGGLWKFNYS"
     gene            413..871
                     /locus_tag="WGQ68_001332"
                     /pseudo
     CDS             413..871
                     /locus_tag="WGQ68_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237797.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
     gene            771..1139
                     /locus_tag="WGQ68_001333"
     CDS             771..1139
                     /locus_tag="WGQ68_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 family protein"
                     /protein_id="HEZ0715119.1"
                     /translation="MAAIGIGMTVLVYGIVAVIVKLDDLGMLLMRRPQTFSRSLGQML
                     TAFMPCFMRGLSVVGTLAMFLIGGVLVAHNLGLLHDFLHAQHWDAGWAEYFANLVVGL
                     LSGSIACAPALPLMNRFGRH"
     gene            1208..2746
                     /gene="lnt"
                     /locus_tag="WGQ68_001334"
     CDS             1208..2746
                     /gene="lnt"
                     /locus_tag="WGQ68_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEZ0715120.1"
                     /translation="MVSKLDKYWQHPALYWPLLILFAAATPFTFAPYYHFWLMPLIFG
                     AFVRLIELRPRFAVSSAYLFGLTAYTTQFYWIHTALHDVSGLPDLYAVPLTFLLPAYL
                     ALYPALCFWLWKKFTLPRGIKIGLVLPILWTLTEFARERFLTGFGWGAIGYSQITPDS
                     PLAGFAPLGGIHMVTLATAFLGVWLVLASDNTTRSGKRLLPIILIAALLAAGYTARQT
                     DFTRPDGSRSTVALLQGNIDQTLKWREDQVIPTIQKYYEQIGKTTADIVILPETAIPV
                     MRQNLPENILAKFAEQAQNNGSALAVGISQYTSDGNGYENAVINLTGYQENNQDGIPY
                     YAKNHLVPFGEYKPLPFLTTPLYKMMNMPLSDFRKGGGKQSALLMKNQKIAFNICYED
                     GFGDELIAAAKDATLLANASNMAWYGKSNAMYQHLQQSQARAMELGRYMVRATNTGAT
                     AIISPKGNIIAQAQPDTETVLEGHIKGYVGETPYMKTGSSWWLMGILTLAALILFIFR
                     NKEH"
     gene            3010..3873
                     /gene="rpoH"
                     /locus_tag="WGQ68_001335"
     CDS             3010..3873
                     /gene="rpoH"
                     /locus_tag="WGQ68_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEZ0715121.1"
                     /translation="MNNAFALPAIQSGNGSLEQYIHTVNSIPMLSQEEETRLAERRIK
                     GDLNAAKQLILSHLRVVVSIARGYDGYGLNQADLIQEGNIGLMKAVKRYEPGRGARLF
                     SFAVHWIKAEIHEFILRNWRLVRVATTKPQRKLFFNLRSMRKNLNALSPKEAQDIADD
                     LGVKLSEVLEMEQRMTGHDIAIMADNSDDEDSFAPIDWLADHDSEPSRQLSKQAHYAL
                     QTEGLQNALAQLDDRSRRIVESRWLQDDGGLTLHQLAAEYGVSAERIRQIEAKAMQKL
                     RGFLTEEAEAV"
     gene            4357..5241
                     /locus_tag="WGQ68_001336"
     CDS             4357..5241
                     /locus_tag="WGQ68_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEZ0715122.1"
                     /translation="MSSGNIHIHSMTGFANAAAECGSKRINLDLRAVNHRFLDIQIRM
                     PDDLRYLESGIREKISSHIARGKVECKIQIQDAENGNQSLELNRDLVGQLAEINKDLR
                     KHYDLAKLGVADILRFPGVLASQRENADALAKVIADLTDSALKDFTAARRREGKKLGE
                     HLLQRLESMEEIIDALSELFPTLLETHKEKIRARLAEAVGSIDNDRLQQEFALFIQKS
                     DIDEEFSRLRTHIAEVRRIVTEHKGSSGKRLDFLMQELNREANTLGSKSIATECTQAS
                     VELKVLIEQMREQVQNIE"
     gene            complement(5325..5840)
                     /locus_tag="WGQ68_001337"
     CDS             complement(5325..5840)
                     /locus_tag="WGQ68_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEZ0715123.1"
                     /translation="MMILKTAPLKRRFAAMLYEMLLVGAATCLAALFAGIAAIFLNPV
                     SIAVSALVTSILIMGSWWLYFRANWHGQGQTLAMRTWKIGLCDLNGIQPSLHLLRLRF
                     IWACIFIVFIPMLAYAGLRHFLGIPPKGAAGAALIWLILPWGFALLNPDRQFLYDFLA
                     GTRLVAVKGKP"
     gene            complement(5915..6334)
                     /locus_tag="WGQ68_001338"
     CDS             complement(5915..6334)
                     /locus_tag="WGQ68_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715124.1"
                     /translation="MDNKTKLRLGGLILLTTAVLSLIIVLIVDSWPLAILLAAVIVAA
                     AAGGFVWTSRRQQRQFIERLKKFDIDPEKGRINEANLRRMYHSGGQHQKDAITLICLS
                     QKCSVDEAHAMFKKRPTRQEINQMAAKQSRGQKRPHR"
     gene            complement(6455..7453)
                     /locus_tag="WGQ68_001339"
     CDS             complement(6455..7453)
                     /locus_tag="WGQ68_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEZ0715125.1"
                     /translation="MAAHIGRIDTDAPLKPLYVIHGEEELLRIEALDALRAAAKKQGY
                     LNREAYTADVSFDWNELLQTAGSAGLFADLKLLELHIPNGKPGKTGGEALQDFAARLP
                     EDTVTLVLLPKLEKTQLQSKWFAALAAKGEVWEAKPVGAAALPQWIRGRLDKIGLGIE
                     ADALALFAERVEGNLLAARQEIDKLGLLYPKGHTVNIDEAQTAVANVARFDAFQLAGA
                     WMKGDVLRVCRLLDGLQEEGEEPVLLLWAVAEDIRTLIRLAAALKQGQSVQSVRNSLR
                     LWGEKQTLAPIAVRRISVVRLLDALKTCAQIDRIIKGAEDGDAWTVFKRLVVSLAE"
     gene            complement(7455..7934)
                     /locus_tag="WGQ68_001340"
     CDS             complement(7455..7934)
                     /locus_tag="WGQ68_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715126.1"
                     /translation="MNKLFLTAAVLMLGACGFHLKGADGISPPLTYRSWHIEGGQALQ
                     FPLETALYQASGRVDDAAGAQMTLRIDSVSQNKETYTVTRAAVINEYLLILTVEAQVL
                     KRGEPVGKPMTVSVRRVLAYADNEILGKQEEEAALWAEMRQDAAEQIVRRLTFLKAE"
     gene            complement(7986..8765)
                     /gene="pgeF"
                     /locus_tag="WGQ68_001341"
     CDS             complement(7986..8765)
                     /gene="pgeF"
                     /locus_tag="WGQ68_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEZ0715127.1"
                     /translation="MKTITETLNLAPKGKNFLTADWPAPANVKTLITTRNGGVSQGAY
                     QSLNLGSHVGDNPDAVRRNREIVQQQVGLPVAYLNQIHSTVVVNAAEALDGTPDADAS
                     VDDTGKVACAVMTADCLPVLFCDRAGTAVAAAHAGWRGLAGGVLQNTIAAMKVPPVEI
                     MAYLGPAISADAFEVGQDVFDAFCTPMPEAAAAFEGIGGGKFLADLYALARLILKREG
                     VGGVYGGTHCTVLERDTFFSYRRDGATGRMASLIWLDGNAV"
     gene            8951..9142
                     /locus_tag="WGQ68_001342"
     CDS             8951..9142
                     /locus_tag="WGQ68_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715128.1"
                     /translation="MFQQGMAHQAPHCVLPERDYPPIRFYRHKQTGFNRKGMGMKSVS
                     DTSRAMPSENQSPLSDGIV"
     gene            9219..10192
                     /locus_tag="WGQ68_001343"
                     /pseudo
     CDS             9219..10192
                     /locus_tag="WGQ68_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217674.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
     gene            complement(10319..11443)
                     /gene="rluD"
                     /locus_tag="WGQ68_001344"
     CDS             complement(10319..11443)
                     /gene="rluD"
                     /locus_tag="WGQ68_001344"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HEZ0715129.1"
                     /translation="MQNTSFDNESDYSDDSDFVSASETENRIGLTVPLELAGGRLDAV
                     LAKLLPDYSRSRLTSWIKEGAVIVNDKPSQPKDKMIGGEQICVTVRPSEENLAFVPEP
                     MALDIVYEDDTVIVVNKPAGLVVHPAAGNWTGTLLNGLLAHCPELSQIPRAGIVHRLD
                     KETSGLMAVAKTLSAQNSLVRQLQERTVKRIYRAVANGIVPFDGKIETQIGRDPHNRL
                     KMAVVKFGGKPAVTHVKVLERYLAHSYIECSLETGRTHQIRVHMREANHPLAGDPVYG
                     NLRHPCGDTVKEAVKSLGARQALHAYRLSFTHPESGETVSFEAPIPNDIYHLLSVLRL
                     EAGLDSSLSNEEEWQDKFGADDDDDWNEDDYDVEVVYVRE"
     gene            11442..12245
                     /locus_tag="WGQ68_001345"
     CDS             11442..12245
                     /locus_tag="WGQ68_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEZ0715130.1"
                     /translation="MKKILLTVSLGLALSACATQGTVDKDAQITQDWSVEKLYAEAQD
                     ELNSSNYTRAVKLYEILESRFPTSRHARQSQLDTAYAYYKDDEKDKALAAIERFRRLH
                     PQHPNMDYALYLRGLVLFNEDQSFLNKLASQDWSDRDPKANREAYQAFAELVQRFPNS
                     KYAADATARMVKLVDALGGNEISVARYYMKRGAYIAAANRAQKIIGSYQNTRYVEESL
                     AILELAYQKLGKPQLAADTRRVLETNFPKSPFLTHAWQPDDMPWWRYWH"
     gene            complement(12303..14522)
                     /locus_tag="WGQ68_001346"
     CDS             complement(12303..14522)
                     /locus_tag="WGQ68_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010357148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEZ0715131.1"
                     /translation="MFRRWFLPCWVVGAAASFALPVVPHWLFWLAAFAVFAVFARRFA
                     FAGLMLCVLAGAAYGVFRTEAALSSQWWAEAVSGVPLTVEVADMPRSGGRRVQFAAKA
                     VDSGGRTFDLLLSDYKRREWAVGSRWRITARVHPVVGELNLRGLNREAWALSNGIGGA
                     GTVGADRVLLHGGSGWGIAVWRSRISRNWQQADADGGLSDGIGLMRALSVGEQSALRP
                     ELWQAFRPLGLTHLVSISGLHVTMVAVMFAWLAKRLLACSPRLPARPRVWVLAAGCAG
                     ALFYALLAGFSVPTQRSVLMLAAFAWAWRRGRLSAWATWWQALAAVLLFDPLAVLGVG
                     TWLSFGLVAALIWACSGRLHEGKRQTALRGQWAASVLSLVLLGYLFASLPLISPLVNA
                     VAIPWFSWVLTPLALLGSVVPFAPLQQLGAFLAEYTLRFLVWLADVSPEFAVASAPLP
                     LLVLAVCAALLLLLPRGLGLRPWAVLLLAGFVFYRPPGVPENEVAVTVWDAGQGLSVS
                     VQTANHHLLFDTGTAAAAQMGIVPSLNAAGVRRLDKLVLSHHDSDHDGGFQAVGKIPN
                     GGIYAGQPEFYEGARHCAEQRWQWDGVDFEFLMPSERKNIDDNGKSCVLRVVADGAAL
                     LVTGDLDTKGEESLVGKYGGNLYSQVLVLGHHGSNTSSSGVFLNAVSPEYAVASSGYA
                     NAYKHPTEAVQNRVRAHGIKLLRTDLSGALQFGLGRGGVKAQRLRGYKFYWQKKPFE"
BASE COUNT         3616 a         4509 c         3663 g         3178 t
ORIGIN      
        1 ggctgtaatt gaatttccac aaaccgccga acactgccgg cggacgcgcg accagcatca
       61 ggaacgccgc aatcgccaag gcttccgccg ccccgtccaa cacgccggcg ggagaaacca
      121 gcagcaaagg cattcccaaa catgcggaca aagtggtcga aaccacgctc aggaacaaca
      181 gtgcgccacc cggcaggaac agactgccca tccaatccac gctaacgatt ctcccttaaa
      241 attcattaaa ttaaaatcga aagactgtaa cacggcattt tttcacgaaa ctttacttca
      301 cgtccgccac cgccttcaga cggcattttc ctgtttcatg ccgcctgaaa ccgcccaaat
      361 ccgcgttctc ctatataatg tcggactttg cgccgcattt caggattctt taatggcatt
      421 tgcctcacta tttaccctgc cggacgacat caccgccgtt ttggacgatg ttgccctgat
      481 gaccaaagcc gccgcaaaga aaaccgccgg catggtcggc gacgatttgg cacccaatgc
      541 caaccgggtt acgggcgtat cggcagaacg cgagttgccg attgtttggt cgatggcgaa
      601 aggttctttt tccaacaagc cggttttggt gcctgcggcc ctgctgcttt ccgccttcct
      661 gccgcaactc attacgccgc ttttgacggc aggcggcatt tatctgtgct ttgaaggcgt
      721 agaaaagctg ctgcacaagt ttctgcaccg acatgacacg catgctcgta atggcggcaa
      781 tcggcatcgg catgactgtt ttggtgtacg gaatcgtcgc cgtcatcgtc aaactcgacg
      841 acttaggcat gctgctgatg cgccgcccac aaaccttttc gcgcagtttg gggcaaatgc
      901 tgactgcctt tatgccttgc tttatgcgcg gtttgagcgt ggtcggcacg cttgccatgt
      961 ttcttatcgg cggcgtactg gtcgcccaca acctcggcct tctgcacgat ttcctgcacg
     1021 cgcaacactg ggacgctggc tgggcggagt acttcgccaa cctcgttgtc gggctgcttt
     1081 ccggttcgat tgcctgcgcc cccgccttgc cgctgatgaa tcgtttcggc aggcattgat
     1141 ttctttttca aataccgatg ccgtttgaaa gatgttcaga cggtatcttc cgaacagaca
     1201 gatgaatatg gtttccaaac tggacaaata ctggcagcac cccgccctct actggccttt
     1261 gctcatcctt tttgccgccg ccaccccctt taccttcgca ccctactacc acttttggct
     1321 gatgcccttg attttcggtg ccttcgtccg cctcatcgaa ctgcgtccgc gttttgctgt
     1381 ctcttccgcc tacctgttcg gcctgaccgc atacacgaca cagttctact ggatacacac
     1441 cgccctgcac gacgtttccg gcctgcccga cctctatgcc gtaccgctga ccttcctact
     1501 ccccgcctac cttgcccttt atccggcact gtgtttctgg ctgtggaaaa aatttaccct
     1561 gcctcggggc ataaaaatcg gtttggtact gcccatcctg tggacgctga ccgagtttgc
     1621 ccgcgaacgt ttcctgaccg gattcggctg gggcgcaatc ggctactccc aaatcacccc
     1681 ggacagcccg ctcgccggct ttgccccatt gggcggcatc cacatggtta cactggcaac
     1741 cgcctttctc ggtgtctggc tggttttggc gagtgataac accacacgtt cgggcaaacg
     1801 cctgcttcca attatcctga ttgccgccct gcttgccgca ggctacaccg cccgacaaac
     1861 cgacttcacc cgccccgacg gcagccgcag caccgtcgcc ctgcttcaag gcaacatcga
     1921 ccaaaccctc aaatggcgtg aagaccaagt catcccgacc atacagaaat attacgaaca
     1981 gatcggcaaa accactgccg acatcgtcat cctgcccgaa accgccatcc ccgtcatgcg
     2041 ccaaaacctg ccggaaaaca tactggcaaa atttgccgaa caggcgcaaa acaacggcag
     2101 cgcgctcgcc gtcggcatca gccaatacac ttcggacggc aacggttacg aaaacgccgt
     2161 catcaacctg accggttatc aggaaaacaa tcaggacggc atcccctact acgccaaaaa
     2221 ccacctcgtc cccttcggcg aatacaaacc gctgcccttc ctgaccacgc cgctttacaa
     2281 aatgatgaat atgccccttt ccgacttccg caaaggcggc ggtaagcaat ccgccctgct
     2341 gatgaaaaac caaaaaatcg ccttcaacat ctgttacgaa gacggattcg gcgacgaact
     2401 gattgccgcc gccaaagatg ccaccctgct tgccaatgcc agcaatatgg cgtggtacgg
     2461 aaaatccaac gccatgtacc agcacctcca acaatcgcag gcgcgggcta tggaactcgg
     2521 acgctatatg gtccgcgcca ccaacaccgg cgcaaccgcc atcatctccc ccaaaggcaa
     2581 catcatcgcc caagcccaac ccgatacgga aaccgtattg gaaggacaca tcaaaggcta
     2641 tgtcggcgaa acgccctata tgaaaaccgg cagttcatgg tggttgatgg gcatattgac
     2701 cctagccgca ctgattcttt tcatcttccg aaacaaagaa cactgatacc tgcacagccc
     2761 gtccggacgg gctgtctttt ttcaaaccgg ctgtttacat acaactacgt atgtaatata
     2821 attccgaaat cggattgagc catcaggttt acaaaatcaa tcaaacctgc cttacaaaat
     2881 tttcctgtct ttcaaacctt agcgcatatc tcctgccaaa catacaaaat acaagccaag
     2941 cagtgatata ctgcatccca acacagcaaa ccgccgatac gcagtttcag ccgaaaggaa
     3001 atgccccaaa tgaataacgc tttcgcatta cccgccatcc aaagcggcaa cggcagcctc
     3061 gaacaataca tccacaccgt caacagcatc ccgatgctgt cccaggaaga agaaacccgc
     3121 ctcgccgaac gccggataaa gggcgacctc aacgccgcca aacaactcat cctgtcccac
     3181 ctgcgtgtcg tcgtttccat cgcgcgcggc tatgacggct acgggctgaa tcaggcagac
     3241 ctgattcagg aaggcaatat cggactgatg aaggcggtca aacgctacga gcccggtaga
     3301 ggtgcacgcc tgttttcatt tgccgtgcat tggattaaag cggaaattca cgaattcatc
     3361 ctgaggaact ggcgccttgt ccgcgttgcc accaccaaac cgcaacgcaa gctgtttttc
     3421 aatttacgca gtatgcgtaa aaacctcaac gccctgtctc ccaaagaggc acaagacatc
     3481 gccgacgatt tgggtgtcaa actttccgaa gttctggaaa tggaacagcg catgacggga
     3541 cacgacatcg ccatcatggc agacaacagc gacgacgagg acagtttcgc ccccatcgac
     3601 tggcttgccg accacgattc cgaaccgagc cgacaactat cgaaacaggc gcattacgcc
     3661 ctgcaaacag aaggtttaca aaacgctttg gcgcaactgg atgacaggag ccgccgtatt
     3721 gtggaaagcc gctggcttca agacgacggc gggctgacgc tgcaccagct tgctgcagaa
     3781 tacggcgtct ctgccgagcg catccgccag attgaagcaa aagccatgca gaaactgcgt
     3841 ggtttcctga ccgaagaagc cgaagcggtt tgaaatatac gcaataaaat acccgacaaa
     3901 ttgttttgtc gggtatttta tttggtaaac atcaatgcgg tttgtgcgtc gtgctgtaca
     3961 cgattttatc cgtccacgcc gttgcaattg ccgaaatcaa aaagcagaca tgcaccagac
     4021 actgccgcat cagctgcttt tccgacagat tggcggaatt gatatagtgg attaacaaaa
     4081 atcaggacaa ggcggcgaag ccgcagacag tacaaatcgt acggaaccga ttcacttggt
     4141 gcttcagcac cttagagaat cgttctcttt gagctaaggc gaggcaacgc cgtactggtc
     4201 ttggttaatc cactatacat ttcccttcct tgcagcaaca aaagcgcgtg attccaatct
     4261 gccgctgcaa aatcttcaat ttttcacacc cgaaaacgct tgccgccata cagatgtgat
     4321 atattgattc cattttaacg ggcttcggag aacgacatga gcagcggaaa tatccacatc
     4381 cacagcatga ccggatttgc caacgcagcg gcagagtgcg gcagcaaacg catcaatctc
     4441 gacctgcgtg cggtcaacca ccgttttcta gatattcaaa tcaggatgcc cgacgatttg
     4501 cgctatttgg aaagcggtat ccgcgagaaa atttcatccc atatcgcacg cggcaaagtc
     4561 gaatgcaaaa tccaaattca agatgcggaa aacggcaatc agtctttgga actgaatcgc
     4621 gatctggtcg gacagcttgc cgaaatcaac aaagacctcc gcaagcatta tgatttggca
     4681 aaactgggcg ttgccgatat tttgcgcttt ccgggcgtat tggcaagtca aagggaaaat
     4741 gcggacgcat tggcaaaggt tattgccgat ttgaccgatt cggcactgaa ggactttacc
     4801 gccgcacgcc ggcgcgaagg gaaaaaattg ggcgagcacc tgttacaacg cctcgaaagc
     4861 atggaagaaa tcatagatgc attaagcgaa ctctttccta ccctgttgga aacacataaa
     4921 gaaaaaatcc gcgcccgcct tgccgaagcg gtcggcagca tcgacaacga ccgcctgcaa
     4981 caggaatttg ccctctttat ccaaaaatcc gacatcgacg aagagttcag ccgcctacgc
     5041 acccacattg ccgaagtgcg ccgtatcgtt accgaacaca aaggcagcag cggcaaacgg
     5101 ctggacttcc tgatgcagga attaaaccgc gaagccaaca ctttgggcag caaatccatc
     5161 gctaccgaat gcacccaagc ctcggtcgag ctgaaagtcc tgatcgaaca gatgcgcgaa
     5221 caggtgcaga atatcgaata atcggtttcc ccgataagca aatgccgtct gaaagccctt
     5281 tcagacggca ttttttcagt ttgaaacctt tgcggtataa aggcttaagg ctttcctttg
     5341 accgccacca atcttgttcc tgcaagaaaa tcatacagaa actgccgatc gggattcagc
     5401 agggcgaacc cccacggtaa aatcagccaa atcaatgccg cgccggccgc gcccttgggc
     5461 ggaatgccga ggaagtggcg caatccggca taagcaagca taggaatgaa gacgatgaat
     5521 atgcacgccc aaataaagcg caggcggagt aagtgtagag acggctgtat gccgttaagg
     5581 tcgcacaaac cgattttcca cgtcctcatc gccaaggtct gtccctgccc gtgccagttg
     5641 gcacggaaat aaagccacca tgatcccatt atcaggatac tcgttaccaa tgcagaaacc
     5701 gcaatagaaa cgggattcag aaaaatggcg gcaataccgg caaacaatgc tgccaaacag
     5761 gttgccgcac cgaccagcag catttcgtac agcatggcgg caaagcggcg tttgagcggg
     5821 gcggttttca gtatcatcat cttttcaatc ggaatatgcc gtctgaaacg ccaacaggct
     5881 tcagacggca ttcatgcaga tatgtctcat gtggttaacg gtgcggacgt ttctgaccgc
     5941 gcgactgttt tgccgccatt tgattgattt cctgacgtgt cgggcgtttt ttgaacatag
     6001 cgtgcgcctc gtccaccgaa catttttgcg acaggcagat cagggtaatc gcatctttct
     6061 ggtgttgtcc gccgctgtgg tacatacggc gcaggtttgc ctcgttgatt ctgccttttt
     6121 cgggatcgat gtcgaatttt ttcaggcgtt cgataaactg gcgttgctgt cggcgggatg
     6181 tccaaacaaa gccgcccgca gcggcggcga cgatgacggc ggcaagcagg atggcaagcg
     6241 gccaggaatc gacaatcaat acgataatga ggcttaaaac ggcggtggtc agtaaaatca
     6301 ggccgcccaa gcgcagtttg gtcttattgt ccatagggtt aatgttcctt tattgaggtg
     6361 gtcgtgccgt ctgaaatgtt ttcggacgga ttggtgaata cttttcagga ttaacggtat
     6421 tttacagtat tttcggattt tggggattac cgctttattc cgccagcgac acgacaagcc
     6481 gtttgaatac cgtccatgcg tcgccgtctt ccgcaccttt gatgattcgg tcgatttggg
     6541 cgcaggtttt gagcgcgtca agcaggcgga cgacggaaat ccgtctgacc gcaatcggag
     6601 cgagcgtctg cttttcaccc caaagtctga gactgttgcg tacggattgg acactctgcc
     6661 cctgtttcaa ggcggcggcc agacggatca gcgtcctgat gtcttcggct accgcccaca
     6721 gcaacagcac cggttcttcg ccttcctcct gcaatccgtc caacaacctg catacgcgca
     6781 ggacatcgcc cttcatccac gcgcctgcca gttggaacgc gtcgaagcgg gcgacgttgg
     6841 caacggcggt ttgcgcctca tcgatattga cggtatgccc tttcggatac agcagcccga
     6901 gcttgtcgat ttcctgacgc gccgccaaca gattgccttc cacgcgctca gcaaacagtg
     6961 ccaatgcgtc tgcctcgata cccaaaccga ttttgtccag ccgtccgcgt atccattggg
     7021 gcaaagccgc cgcgccgacc ggtttggctt cccacacttc cccctttgcc gccaatgcgg
     7081 caaaccattt ggactggagc tgggttttct ccagtttggg cagtaaaacc agcgttaccg
     7141 tatcttccgg caggcgggcg gcaaaatcct gcaacgcctc gccgccggtt ttgccgggct
     7201 tgccgttagg gatatgcagt tccaacagct tcaaatcggc aaacagaccc gcactgcctg
     7261 cggtttgcag cagttcgttc caatcgaaag acacgtcggc ggtataggct tcgcggttga
     7321 gataaccctg cttcttcgcc gccgccctca atgcgtccaa tgcctcgata cgcaacagtt
     7381 cttcctcgcc gtggatgacg tacagaggtt tcaaaggcgc gtccgtatca atgcgtccga
     7441 tatgtgccgc cacgtcattc cgccttcaga aaggtcaggc ggcggacaat ctgttcggcg
     7501 gcatcctgcc gcatttccgc ccacaatgcc gcttcctctt cctgtttgcc caagatctcg
     7561 ttgtcggcat aagcaaggac gcggcggacg gacacggtca tcggtttgcc gaccggctcg
     7621 ccgcgtttca atacctgcgc ttcaaccgtc aagattaaaa gatattcgtt gatgactgcc
     7681 gcacgggtaa cggtgtaggt ttccttgttt tgggaaacgc tgtctatacg cagggtcatc
     7741 tgcgcgccgg cagcatcgtc caccctgccc gaagcctgat acagcgcggt ttccaaagga
     7801 aactgcaatg cctgtccgcc ttcgatgtgc cagctccggt aggtcagcgg cggagaaatg
     7861 ccgtctgcac ctttcaggtg gaaaccgcac gcgcccagca tcagcactgc agcagtaaga
     7921 aacagtttgt tcatggcttg cctttctcaa aagcacaaag tcggtagatt atatcagcgg
     7981 cgtgctcaga cggcattgcc gtccagccaa atcaggctcg ccatacgccc tgtcgctccg
     8041 tcgcggcggt aggaaaagaa agtatcccgt tccaaaaccg tacaatgcgt gccgccgtat
     8101 acgccgccta cgccttcgcg cttcagaatc aggcgggcga gcgcgtaaag gtcggcaagg
     8161 aatttgccgc cgcctatgcc ttcaaatgcg gcggcggctt cgggcatggg cgtgcagaac
     8221 gcatcaaaca cgtcctgccc gacttcaaac gcatccgcac tgatggcagg gccgagatac
     8281 gccataattt cgacaggcgg aaccttcatc gcggcaatgg tgttttgcaa tacgccgccc
     8341 gccaaaccgc gccagcccgc gtgtgcggcg gcaaccgccg tacctgccct gtcgcaaaat
     8401 agaacgggca gacagtcggc agtcatgaca gcacaggcaa ccttgcccgt gtcgtccacg
     8461 gaagcgtcgg catcgggcgt gccgtccaat gcttcggcag cgttgacgac gacggtgctg
     8521 tggatttgat tgaggtaggc aacgggcaat ccgacctgct gttgcacgat ttcgcgattg
     8581 cggcgtacgg catcgggatt gtcgccgacg tgcgaaccta agttcaaact ttgatacgcg
     8641 ccctggctca cgccgccgtt gcgcgtggta atcagggttt tcacattggc gggcgcgggc
     8701 caatcggcgg tcaggaagtt tttgcctttc ggggcaagat ttagggtttc tgtgatggtt
     8761 ttcattggat attggaatag gtgtgtggcg gaaagggtta attaactgcc ttcggggcaa
     8821 atggatgttt gttttttcgt cattcccgca caggcgggaa ttcggagatt cgggattgtt
     8881 ttcaaacgtt cgggttcgga ttcttgccgg tgcgggaatg atgtccttat atactttctc
     8941 caagctttat atgtttcaac aggggatggc acatcaagca ccgcactgcg tgttgcccga
     9001 acgagactac cctccgatta gattctatcg ccataaacag acgggtttca accgaaaagg
     9061 aatgggaatg aagtccgttt ccgacacctc tcgggcgatg ccgtctgaaa accaatctcc
     9121 actttcagac ggcattgttt aggcgcagtc attttgcaaa ttaaggttaa ggcgtgtatg
     9181 cttgctttcc cttaaaaaca aacacgcaac gcattattat gaatatcctc agtaaaatca
     9241 gcagctttat cggaaaacat tttccctctg ggtcgcgctc tttgccgccg ccgctttttt
     9301 cgcgcccgac accttcaaat gggcggggcc ttatattcct tggctgttgg gcattattat
     9361 gttcggtatg ggtttgacgc tcaaaccttc cgacttcgat attttgttca aacatcccaa
     9421 agccgtcatc atcggcgtaa tcgcacaatt cgccattatg ccggcaaccg cctggctgct
     9481 gtccaaactg ttgaacctgc ctgccgaaat cgcggtcggc gtgattttgg tcggctgctg
     9541 cccgggcggt acggcttcca atgtgatgac ctatctggca cgcggcaatg tggctttgtc
     9601 ggttgccgtt acgtctgttt ccaccctgat ttccccattg ctgactcccg ccatcttcct
     9661 gatgcttgcc ggcgaaatgc tggaaatcca agcggccggt atgttgatgt ccatcgtcaa
     9721 aatggttttg ctccccattg ttttgggttt gattgtccat aaggttttgg gtagcaaaac
     9781 cgaaaagctg accgatgcgc tgccgctggt ttccgttgcc gccatcgtgc tgattatcgg
     9841 cgcggttgtt ggggcaagca aaggcaagat tatggaaagc ggcctgctga tttttgcggt
     9901 tgtcgtactc cacaacggca tcggctacct gctcggcttc tttgccgcca aatggaccgg
     9961 cctgccttat gatgcacaaa aaacgctgac catcgaagtc ggtatgcaaa actcgggcct
    10021 ggccgccgcg cttgccgccg cacactttgc cgccgcgccg gtcgttgccg ttccgggcgc
    10081 attgttcagc gtgtggcaca atatctccgg ctcgctgctg gcaacttatt gggcagccaa
    10141 agccggtaaa cataaaaaaa ccctaaacgc ctttcatcat aatgccgtct gaacggggca
    10201 aacaaaaccg gcggtttctg cccggcccct tcagacggcc tttgtttgaa atcggcgggg
    10261 acgattgtcc ggctgctccg attcggctgc ctgcgttcgc cccgcctttc aagccgcctc
    10321 actcccttac ataaaccact tcgacatcat agtcgtcttc gttccaatca tcgtcgtcgt
    10381 ccgcgccgaa tttgtcctgc cattcctctt cattgctcaa agacgaatcc aaaccggctt
    10441 caagacggag gacggacaac aaatgatata tgtcgtttgg aatcggtgct tcaaacgaaa
    10501 cggtttcgcc gctttccgga tgagtgaaac ttaagcggta ggcgtgcaat gcctgacgcg
    10561 cgcccagact tttaacggct tctttcaccg tgtcgccgca cgggtggcgc aggttgccgt
    10621 aaaccgggtc gccggcaagc ggatggttag cttcgcgcat atgtacgcgg atttggtgcg
    10681 tcctgcctgt ttcgagcgaa cactcgatat agctgtgggc aagatagcgt tccaacactt
    10741 tgacgtgggt aacggctggt ttgccgccga atttgacgac tgccattttc aggcggttgt
    10801 gcggatcgcg cccgatttgg gtttcaattt taccatcaaa gggaacgatg ccattggcga
    10861 cggcgcggta gatgcgtttg actgtgcgtt cctgaagctg ccgcacaagg gaattttgcg
    10921 cggacagggt tttggcgacc gccatcagcc cgctggtttc cttgtccaaa cggtgtacga
    10981 tgcccgcgcg cggaatttgg gacagctcgg ggcaatgcgc caacaggccg ttgagcagcg
    11041 tccccgtcca gttgcccgcc gccggatgca ccaccagtcc ggccggtttg ttgacgacga
    11101 tgacggtatc gtcttcgtaa acaatatcca aagccatagg ctctggaaca aacgccagat
    11161 tttcctcact cggacggacg gttacacaaa tttgctcgcc gcctatcatt ttgtctttgg
    11221 gttgcgaagg tttatcgttt acaataaccg cgccttcttt aatccatgat gtcaggcggc
    11281 tgcgcgagta gtcgggcaga agtttcgcca ataccgcatc caaccgcccg ccggcaagct
    11341 cgagcggaac ggtcagaccg atacggtttt cagtttcgga agctgacaca aagtctgaat
    11401 catcgctata atcggattca ttatcaaagg aagtattctg catgaaaaaa attcttttaa
    11461 cggtttcatt aggtttggcg ctgagtgcct gtgccactca aggtacggta gataaagatg
    11521 cccagattac ccaagactgg agtgtggaaa aactctatgc cgaagcccag gatgagctga
    11581 acagcagcaa ttatactcga gctgtcaaat tatacgaaat cttggaatcg cgcttcccca
    11641 ccagccgcca tgcccggcag tcgcaactgg ataccgcata cgcctattat aaagacgatg
    11701 aaaaagacaa agctctggcg gcaatcgaac gcttccgccg ccttcacccg caacacccga
    11761 atatggatta cgcgctgtat ctgcgcggct tggtgctgtt caacgaagac cagtccttct
    11821 tgaacaaact ggcctcgcaa gactggtccg accgcgaccc gaaagccaac cgcgaagcgt
    11881 accaggcgtt tgcggaactc gtccaacgct tcccgaacag caaatacgcc gccgatgcaa
    11941 ccgcacgcat ggtcaaactg gtcgatgcac tgggcggcaa tgaaatatcg gtggcgcgct
    12001 actacatgaa acgcggtgca tacatcgccg ccgccaaccg cgcccaaaaa atcatcggca
    12061 gctaccagaa tacgcgttat gtcgaagaat cgctcgccat attggaactt gcctaccaaa
    12121 aactcggcaa accacagctt gccgccgata cgcgccgcgt gttggaaacc aacttcccga
    12181 aaagcccgtt tttgacgcac gcttggcagc ccgacgatat gccctggtgg cgttactggc
    12241 attaagccca tcttcgtaat gccgtctgaa gccgtttcag acggcatttt atgtttcaaa
    12301 cctcattcaa acggtttttt ctgccaatag aatttatacc ctctcaaacg ttgagccttc
    12361 acgccgccgc gtcccaagcc gaattgcagt gcgcccgaca aatcggtacg cagcaatttg
    12421 atgccgtgtg cgcggacacg gttctgcacc gcttcggtcg gatgtttgta ggcgttggca
    12481 taaccgcttg aagcaacggc atattcgggc gagacggcat tgaggaacac gcccgacgag
    12541 gacgtattgc tgccgtgatg tcccaaaacc aaaacctggc tgtacaggtt gcctccatac
    12601 ttgccgacca ggctttcctc gcccttcgta tccaaatcgc ccgttaccag cagtgccgca
    12661 ccatcagcca caacacgcaa aacacaactt ttcccattat catcgatgtt tttgcgttca
    12721 gacggcatca aaaactcgaa atccacgccg tcccattgcc aacgctgttc cgcacaatgc
    12781 cgcgccccct cataaaattc cggctgcccg gcataaatcc cgccgttcgg tatcttgccg
    12841 actgcctgaa aaccgccgtc gtggtcgctg tcgtgatgcg acaaaaccag cttgtccaaa
    12901 cggcggacac ccgccgcatt caaactcggc acaatcccca tctgcgccgc cgctgccgtt
    12961 ccggtgtcaa acaaaagatg atgatttgcc gtctgaaccg acaccgacaa accctgcccc
    13021 gcatcccaaa ccgtaaccgc aacctcattt tccggcacgc cgggcggacg gtaaaacaca
    13081 aaccctgcca acagcaacac cgcccacgga cgcaaaccca agccgcgcgg cagcagcaac
    13141 agcaaagcgg cacacaccgc caacaccaac agcggcaaag gtgcggaggc aacggcaaac
    13201 tcgggcgaca catcggcaag ccacaccaaa aaccgcaaag tatattccgc caaaaatgcc
    13261 cccaactgtt gcaaaggcgc aaacggcacg accgaaccca gcaacgccag cggcgtcaat
    13321 acccaagaaa accacggaat cgccaccgca ttgaccaaag ggctgattaa aggcagcgaa
    13381 gcaaacagat aaccgagcaa aaccaacgac aacaccgaag ccgcccactg cccgcgcaaa
    13441 gcagtttgcc gttttccctc gtgcaaacgc cccgagcacg cccatatcag ggccgccacc
    13501 aaaccgaaag acagccaagt ccccacaccc aagaccgcca acgggtcgaa cagcagcacc
    13561 gccgccaacg cctgccacca cgttgcccac gccgacaatc ttcccctgcg ccacgcccac
    13621 gcaaacgccg ccaacatcaa aacgctgcgc tgcgtcggca cggaaaaacc cgcaagcagc
    13681 gcgtaaaaca gcgcgcctgc acaccccgcc gccaaaaccc acacgcgcgg ccgggcagga
    13741 aggcgcgggg aacaggcaag cagccgcttc gccagccacg caaacattac cgccaccatc
    13801 gtaacgtgca aacccgaaat actgaccaaa tgcgtcagcc ccaacggccg gaacgcctgc
    13861 cacaattcgg ggcgcaatgc cgactgttcg cccacgctca acgcgcgcat cagcccgatg
    13921 ccgtctgaaa gcccgccgtc cgcatccgcc tgctgccaat tacggctgat gcggctgcgc
    13981 caaaccgcaa tcccccaacc gcttccgcca tgcagcaaaa ccctgtccgc accgaccgtc
    14041 cccgcgccgc ctattccgtt ggataatgcc cacgcctcac ggttcaaacc gcgcaggttc
    14101 aactccccga cgacagggtg cacacgtgcc gttatccgcc atctgctccc gaccgcccat
    14161 tcgcgccgtt tgtagtccga cagcagcaaa tcaaacgtcc gaccaccgct gtccacagcc
    14221 tttgccgcaa actgcacacg ccgcccgccc gacctcggca tatccgccac ttccaccgtc
    14281 aaaggcacgc ctgaaacggc ttctgcccac cattgggaag acaatgccgc ctccgtcctg
    14341 aatacgccgt aagccgcgcc cgccaacacg cacagcatca gaccggcaaa cgcaaaacgc
    14401 cttgcaaaca cagcaaaaac cgcaaaagcc gccagccaaa acagccaatg cggcacgacc
    14461 ggcagcgcga acgaagccgc cgcaccgaca acccaacacg gcaaaaacca acgacggaac
    14521 acgtctcccc accccagata tttcaaaaat aaacggataa taaagcaaat ccgaaaaacc
    14581 gtcataacaa gatttgaaaa tgatatagtg gtttaaattt aaatcaggac aaggcgacga
    14641 agccgcagac agtacaagca gtacgacaag gcgaggcaac gctgtactgg tttttgttaa
    14701 accactataa gaatgcaaac gaaaagcctc agtattatcc gagacaacgg caaactttcc
    14761 atcgtcattc ccacgaaccc acatcccgtt attaccacga aagcgggaat ctaggactca
    14821 gggttaagaa aacctacatc ccgtcattcc ctcaaaaaca gaaaaccaaa atcagaaacc
    14881 taaaatcccg tcattcccac gaaagtggga atctaggtct gtcggtgcgg aaacttatcg
    14941 ggtaaaacgg tttctttaga ttccac
//
LOCUS       DAWFHY010000051        14500 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.051, whole genome shotgun sequence.
ACCESSION   DAWFHY010000051 DAWFHY010000000
VERSION     DAWFHY010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14500
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.051"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(133..477)
                     /locus_tag="WGQ68_001347"
     CDS             complement(133..477)
                     /locus_tag="WGQ68_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HEZ0715132.1"
                     /translation="MNKQYPKINYIGNKEKIASWICDQLPSDVDTVADVFSGGCSFAY
                     EAKKRGYRVITNDILAINYQIALALIENNHETLNDDDVAMIFSGSPHAGFMSQRYAEK
                     FYFHDEYQQLDL"
     gene            complement(470..1472)
                     /gene="dcm"
                     /locus_tag="WGQ68_001348"
                     /pseudo
     CDS             complement(470..1472)
                     /gene="dcm"
                     /locus_tag="WGQ68_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239422.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(1503..3776)
                     /locus_tag="WGQ68_001349"
     CDS             complement(1503..3776)
                     /locus_tag="WGQ68_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEZ0715133.1"
                     /translation="MNITQILSQELSATAAQITAAVELLDDGATVPFIARYRKEATGG
                     LDDTQLRRLAERLQYLRELEERKAVVLKSIEEQGKLSDDLRAQIEAADNKTALEDLYL
                     PYKPKRRTKAQIAREHGLQPLADVLLAEQSQDVEAATQGYLNENVPDAKAALDGARAI
                     LMEQFAEDAELIGTLRDKLWNEAEIHAQVVGGKETEGEKFSDYFDHREPIRAMPSHRA
                     LAVLRGRNEGVLNIALKYQPDDTPITQQSEYEQIIARRFKVSDGHKWLRDTVRLTWRA
                     KIFLSLELEALNRLKEAADTDAITVFARNLKDLLLAAPAGRLTTLGLDPGYRNGVKCA
                     VVDDTGKLLDTVIVYLHQENNMLATLSRLIKQHGVKLIAIGNGTASRETDKIAGELVR
                     EMSKMGLHKIVVSEAGASIYSASELAAREFPDLDVSLRGAVSIARRLQDPLAELVKID
                     PKSIGVGQYQHDVNQSQLAKSLDAVVEDCVNAVGVDVNTASAPLLARISGLNQTLAQN
                     IVAYRDENGAFDSRKKLLKVPRLGEKTFEQAAGFLRINGGTEPLDASAVHPEAYPVVA
                     KMLAQQGITAAELIGNRERVKQIKASDFTDERFGLPTILDILSELEKPGRDPRGEFQT
                     ASFAEGIHEISDLQVGMILEGVVSNVANFGAFVDIGVHQDGLVHISALSNKFVQDPRE
                     VVKAGDVVKVKVLEVDAARKRIALTMRLDDEPGGAKHKMPSENRSRERTAGRKPQRND
                     RAPANSAMADAFAKLKR"
     gene            complement(3840..4490)
                     /locus_tag="WGQ68_001350"
     CDS             complement(3840..4490)
                     /locus_tag="WGQ68_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0715134.1"
                     /translation="MISVEHVSKRYLTRQGWRTVLHDISFKMEKGEKIGILGRNGAGK
                     STLIRLISGVEPPTTGEIKRTMSISWPLAFSGAFQGSLTGMDNLRFICRIYNVDIDYV
                     KNFTEEFSELGQYLYEPVKRYSSGMKARLAFALSLAVEFDCYLIDEVIAVGDSRFADK
                     CKYELFEKRKDRSIILVSHSHSAMKQYCDNAMVLEKGHMYQFEDMDKAYEYYNSLP"
     gene            complement(4487..5284)
                     /locus_tag="WGQ68_001351"
     CDS             complement(4487..5284)
                     /locus_tag="WGQ68_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_157140410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEZ0715135.1"
                     /translation="MKALHKTSFWESLAIQRRVIGALLMREIITRYGRNNIGFLWLFV
                     EPLLMTFVIVLMWKFLKADRYSTLNIVAFAITGYPMLMMWRNASKRAVGSISSNASLL
                     YHRNVRVLDTILARMILEIAGATIAQIVIMAVLIAIGWIEMPADMFYMLMAWLLMAFF
                     AIGLGLVICSIAFNFEPFGKIWGTLTFVMMPLSGAFFFVHNLPPKVQEYALMIPMVHG
                     TEMFRAGYFGDSVTTYENPWYILLCNLVLLLLGLAVVARFSKGVEPQ"
     gene            complement(5284..6447)
                     /gene="ctrB"
                     /locus_tag="WGQ68_001352"
     CDS             complement(5284..6447)
                     /gene="ctrB"
                     /locus_tag="WGQ68_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export protein CtrB"
                     /protein_id="HEZ0715136.1"
                     /translation="MSEQLPVAVATETKAERKKPKKKSWIKKLSPLFWVTVIIPTVIS
                     LVYFGFFASDRFTSQSSFVVRSPKSQSSLNGLGAILQGTGFARAQDDIYTVGEYMRSR
                     SSLDELRKILPVREFYETKGDVFSRFNGFGFRGEEEAFYQYYKNQVMINFDTVSGIST
                     LNVTSFDALESKKINEALLKQGEALINQLNDRARADTVRYAEEVVKTAAERVKEASQN
                     LTDYRIANGVFDLKAQSEVQMGLVSKLQDELIVIQTQLDQVKAVTPENPQIPGLQARE
                     QSLRKEIDQQLRAISGGGHSSLSNQAAEYQRVYLENQLAEQQLAAAMTSLESAKVEAD
                     RQQLYLEVISQPSLPDLAHEPKRLYNIVATLIIGLMVYGILSLLTASIREHKN"
     gene            complement(6462..7637)
                     /gene="ctrA"
                     /locus_tag="WGQ68_001353"
     CDS             complement(6462..7637)
                     /gene="ctrA"
                     /locus_tag="WGQ68_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule polysaccharide export outer membrane
                     protein CtrA"
                     /protein_id="HEZ0715137.1"
                     /translation="MFKVKFYIRHAVLLLCGSLIVGCSAIPSSGPSAKKIVSLGQQSE
                     VQIPEVELIDVNHTVAQLLYKAQINQSFTQFGDGYASAGTLNIGDVLDIMIWEAPPAV
                     LFGGGLSSMGSGSAHQTKLPEQLVTARGTVSVPFVGDISVVGKTPGQVQEIIKGRLKK
                     MANQPQVMVRLVQNNAANVSVIRAGNSVRMPLTAAGERVLDAVAAVGGSTANVQDTNV
                     QLTRGNVVRTVALEDLVANPRQNILLRRGDVVTMITNPYTFTSMGAVGRTQEIGFSAR
                     GLSLSEAIGRMGGLQDRRSDARGVFVFRYTPLVELPAERQDKWIAQGYGSEAEIPTVY
                     RVNMADAHSLFSMQRFPVKNKDVLYVSNAPLAEVQKFLSFVFSPVTSGANSINNLTN"
     gene            7772..8905
                     /gene="neuC"
                     /locus_tag="WGQ68_001354"
     CDS             7772..8905
                     /gene="neuC"
                     /locus_tag="WGQ68_001354"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEZ0715138.1"
                     /translation="MKRILCITGTRADFGKLKPLLAYIENHPDLELHLIVTGMHMMKT
                     YGRTCKEVTRENYQHTYLFSNQIQGEPMGAVLGNTITFISRLSDEIEPDMVMIHGDRL
                     EALAGATVGALSSRLVCHIEGGELSGTVDDSIRHSISKLSHIHLVANEQAVTRLVQMG
                     EKRKHIHIIGSPDLDVMASSTLPSLEEVKEYYGLPYENYGISMFHPVTTEAHLMPQYA
                     AQYFKALELSGQNIISIYPNNDTGTESILQELLKCQSEKFIAFPSIRFEYFLVLLKHA
                     KFMVGNSSAGIREAPLYGVPSIDVGTRQSNRHMGKSIIHTDYETKNIFDAIQQACSLG
                     KFEADDTFNGGDTRTSTERFAEVINNPETWNVSAQKRFIDLNL"
     gene            8909..9595
                     /gene="neuA"
                     /locus_tag="WGQ68_001355"
     CDS             8909..9595
                     /gene="neuA"
                     /locus_tag="WGQ68_001355"
                     /EC_number="2.7.7.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acylneuraminate cytidylyltransferase"
                     /protein_id="HEZ0715139.1"
                     /translation="MEKQNIAVILARQNSKGLPLKNLRKMNGISLLGHTINAAISSKC
                     FDRIIVSTDGGLIAEEAKNFGVEVVLRPAELASDTASSISGVIHALETIGSNSGTVTL
                     LQPTSPLRTGAHIREAFSLFDEKIKGSVVSACPMEHHPLKTLLQINNGEYAPMRHLSD
                     LEQPRQQLPQAFRPNGAIYINDTASLIANNCFFIAPTKLYIMSHQDSIDIDTELDLQQ
                     AENILNHKES"
     gene            9596..10645
                     /gene="siaC"
                     /locus_tag="WGQ68_001356"
     CDS             9596..10645
                     /gene="siaC"
                     /locus_tag="WGQ68_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysialic acid capsule biosynthesis protein
                     SiaC"
                     /protein_id="HEZ0715140.1"
                     /translation="MQNNNEFKIGNRSVGYNHEPLIICEIGINHEGSLKTAFEMVDAA
                     YNAGAEVVKHQTHIVEDEMSDEAKQVIPGNADVSIYEIMERCALNEEDEIKLKEYVES
                     KGMIFISTPFSRAAALRLQRMDIPAYKIGSGECNNYPLIKLVASFGKPIILSTGMNSI
                     ESIKKSVEIIREAGVPYALLHCTNIYPTPYEDVRLGGMNDLSEAFPDAIIGLSDHTLD
                     NYACLGAVALGGSILERHFTDRMDRPGPDIVCSMNPDTFKELKQGAHALKLARGGKKD
                     TIIAGEKPTKDFAFASVVADKDIKKGELLSGDNLWVKRPGNGDFSVNEYETLFGKVAA
                     CNIRKGAQIKKTDIE"
     gene            10729..12216
                     /locus_tag="WGQ68_001357"
     CDS             10729..12216
                     /locus_tag="WGQ68_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2,8-polysialyltransferase family protein"
                     /protein_id="HEZ0715141.1"
                     /translation="MLKKIKKALFQPKKFFQDSMWLTTSPFYLTPPRNNLFVISNLGQ
                     LNQVQSLIKIQKLTNNLLVILYTSKNLKMPKLVHQSANKNLFESIYLFELPRSPNNIT
                     PKKLLYIYRSYKKILNIIQPAHLYMLSFTGHYSYLISIAKKKNITTHLIDEGTGTYAP
                     LLESFSYHPTKLERYLIGNNLNIKGYIDHFDILHVPFPEYAKKIFNAKKYNRFFAHAG
                     GISINNNIANLQKKYQISKNDYIFVSQRYPISDDLYYKSIVEILNSISLQIKGKIFIK
                     LHPKEMGNNYVMSLFLNMVEINPRLVVINEPPFLIEPLIYLTNPKGIIGLASSSLIYT
                     PLLSPSTQCLSIGELIINLIQKYSMVENTEMIQEHLEIIKKFNFINILNDLNGVISNP
                     LFKTEETFETLLKSAEFAYKSKNYFQAIFYWQLASKNNITLLGHKALWYYNALYNVKQ
                     IYKMEYSDIFYIDNISVDFHSKDKLTWEKIKHYYYSADNRIGRDR"
     gene            complement(12712..13665)
                     /locus_tag="WGQ68_001358"
     CDS             complement(12712..13665)
                     /locus_tag="WGQ68_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715142.1"
                     /translation="MHQYYDAVILSVGNGLLKRFFKQNAQLNIASRPLIITLFPGVVF
                     GDQASILSRMGADIVLYNNKHDFRIAEEYKKQYKLSCQNILYGYPIFRHASKGRHGEK
                     IYFIDQVKIPFKKEERIYTLKKLIALAEKYPEKEFTILLRVADKDITVHQDKHSYIEL
                     AKQFQLPSNLTIERKSTAQAFQEMGYCLSYSSTMLFEAECKGIPVGVVADLGFSKSYA
                     NQHFLGSGVLVYFDQIDFTSPKIADPDWLDCYATKKVITTDEFNKLLKQVVPLQHDYQ
                     EYLSAVNSIESTKTIFLRKFKKLIRDPKKFFYDSKWLRKVI"
     gene            14048..>14500
                     /locus_tag="WGQ68_001359"
     CDS             14048..>14500
                     /locus_tag="WGQ68_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0715143.1"
                     /translation="MKKILVTGGTGFIGSHTVVSLLKSGHQVVILDNLCNSSINILPR
                     LKTITGQEIPFYQGDIRDREILRRIFAENRIDSVIHFAGLKAVGESVAEPMKYYDNNV
                     SGSLVLAEEMARAGVFSIVFSSSATVYGDPGKVPYTEDMPLGDTTSPYG"
BASE COUNT         4286 a         3293 c         2839 g         4082 t
ORIGIN      
        1 gccataagga ttagcggtat cgcccgggcg catatcttcc gtatagggca ttttacgtat
       61 catggcgcga cgcattaaag taaacgccaa agcgcgttta tactggttat ccagtttccc
      121 tatattttta cgttacaaat caagttgttg gtattcatcg tgaaaataga atttttcggc
      181 ataacgctga ctcataaaac cggcatgcgg gctgcctgaa aaaatcattg cgacatcatc
      241 gtcatttaat gtttcatggt tgttttctat taatgctaaa gcaatttggt aattaattgc
      301 caaaatatcg ttagtaatca cacgatagcc gcgttttttg gcttcgtagg caaaggaaca
      361 gcctccacta aatacatctg caactgtatc tacatcagac ggaagctggt cacaaatcca
      421 ggaagctatt ttctctttat taccgatata gttaattttc ggatattgct tattcatttt
      481 cactcatttt taaaatactt tcagcaatag cttttgccaa taaaggcggt acggcgttac
      541 caacctgctg ctgctgggca attttgctgc cgcaaaaaat aaaattatca gggaaagatt
      601 gtaaggcagc taattcacga acggttaacg cccgattctg ttcatagtga aaaactttgc
      661 gcatatctcc tgtaatacaa acggctggtt ttgttgctgt tgtaacggat gtatttacgg
      721 atatcacctg ttttcggacg taatggttca ggaatatcgt tacggttacc tccattttta
      781 acaaatgcca ttttttctaa catttgtgcc gaatgattca tagcttcatg atttgcaacg
      841 tgtggattgc tttcgccagc agccagtttt ggaaaatgtc ctattgctga tccaacagtc
      901 tgatgggaaa tctgcaaagg ttcgggaaag gaaattttgc ctttatccct cctcccgata
      961 aatatcactc ggctacgtat ctgaggaaca ccgaaatcgg ctgcactcag tatcttacat
     1021 tccaccgaat aaccgatatt ctgaaatgct tgaataatct caatacgtgt tttacctgaa
     1081 ttgtgtgtat agagtcgcgc tacattttcc ataacaaaaa aatatggttg gacaatttta
     1141 actattcgga caaactcttt aaataaatgg ttgcgtgggt catctgtaaa tgtccgtcca
     1201 atctttcctg ccatactaaa accttgacaa ggtggtcctc caataatcaa atcaactgct
     1261 tgtccgttaa gacaattgat taaatcttgt tcggttagtg tggttaaatc tttttgcagt
     1321 aattgatgat gggggaagtt ggtacggtaa gtctgacaat aatcagactc catttcaaca
     1381 gaaagcaatt gttggaatcc ggcttgttca aaacccaagg ataggcctcc tgctcctgag
     1441 aaaaggtcaa tataggtaag tggtgtgtgc ataagtcaaa atccataaac tcttcgatta
     1501 ttttaccgct tcagcttcgc aaacgcatcc gccatcgccg aattggctgg ggcgcggtcg
     1561 ttgcgttggg gtttgcggcc ggctgtccgt tcgcggctgc ggttttcaga cggcatttta
     1621 tgttttgcgc cgcccggttc gtcatccaag cgcatggtca gcgcgatgcg tttgcgtgca
     1681 gcatcgactt ccagcacttt cactttcacc acgtcgccag ctttcaccac ttcgcgcggg
     1741 tcttggacga acttgttgga cagggcggag atgtgcacca agccgtcctg atggacgccg
     1801 atgtccacga acgcgccgaa gttggcgacg ttggaaacca cgccttcgag tatcataccg
     1861 acttgcaagt cgctgatttc gtggatacct tcggcaaacg atgccgtctg aaactcgccg
     1921 cgcggatcac ggccgggttt ttccagttcg gacaggatgt ccaaaatggt cggcaggccg
     1981 aagcgttcgt cggtgaagtc ggacgctttg atttgcttca cgcgctcgcg gttgccgatg
     2041 agttcggcgg cggtaatgcc ttgttgcgcc agcattttgg cgacgacggg ataggcttcg
     2101 gggtggacgg cgctcgcgtc cagcggctct gtgccgccgt taatccgcaa aaagcctgcc
     2161 gcctgctcga aggttttttc gcccaaacgc ggtactttca gcaatttttt gcggctgtcg
     2221 aacgcgccgt tttcatcgcg gtaggcaacg atgttttggg caagggtttg attcaaaccg
     2281 gaaattcgcg ccaagagcgg ggcggaggcg gtgttcacgt ccacgccgac ggcgttcacg
     2341 cagtcttcga ccaccgcgtc cagcgatttg gcgagctggc tttggttcac gtcgtgctga
     2401 tactgcccca cgccgatgga tttggggtcg attttgacca actcggcaag cgggtcttgt
     2461 agcctgcggg cgatggacac cgcgccgcgc agggaaacgt ccaagtcggg gaactcgcgc
     2521 gccgccagtt cggacgcgga ataaatcgac gcgccggctt cggacacgac gattttatgc
     2581 agccccattt tcgacatttc acgcaccagt tcgcccgcga ttttgtcggt ttcacggctg
     2641 gcggtgccgt taccgatggc gatgagcttc acgccgtgct gtttaatcag gcgcgacagc
     2701 gttgccaaca tattgttttc ttgatgcaaa tagacgatga cggtatccag cagcttgccg
     2761 gtgtcgtcca ccacggcgca tttcacgccg ttgcggtagc cggggtcgag acccagcgtg
     2821 gtcagccgtc cggcgggcgc agcaagcagc aagtctttga gattgcgggc gaacacggta
     2881 atcgcgtcgg tgtcggcggc ttctttcaaa cgattgagag cttcaagttc caacgacaaa
     2941 aagattttcg cgcgccaagt caggcgcacg gtgtcgcgca gccatttgtg gccgtctgaa
     3001 actttgaagc ggcgggcgat gatttgctcg tattcgcttt gctgcgtaat cggcgtgtcg
     3061 tcgggctgat atttgagcgc gatgttcaac acgccttcgt tgcgtccgcg caaaaccgct
     3121 agcgcacgat ggctgggcat cgcacgaata ggttcgcggt ggtcaaaata atcgctgaat
     3181 ttttcgcctt cggtttcttt gcctccaaca acttgcgcgt ggatttcggc ttcgttccac
     3241 agcttgtcgc gcagcgtgcc gatgagttcc gcgtcttcgg caaactgctc catcagaatc
     3301 gcacgcgcgc cgtccaacgc ggctttggca tcggggacgt tttcgttcag gtagccttgt
     3361 gtggcggctt ccacgtcctg cgactgctcg gcaagcaaca cgtccgccag cggctgcaaa
     3421 ccgtgttcgc gcgcaatttg cgccttggtg cggcgtttgg gcttgtaggg cagatacagg
     3481 tcttccagcg cggttttgtt gtcggcggct tcgatttgcg ccctgaggtc gtctgaaagt
     3541 ttgccttgct cttcaatgct ttttaacaca acggctttgc gctcttccaa ctcgcgcaga
     3601 tattgcagcc gctcggcaag ccggcgcaac tgcgtatcgt ccaacccgcc cgtcgcttcc
     3661 ttgcggtagc gggcgataaa cggcacggtc gcgccgtcgt ccaaaagctc gacggcggcg
     3721 gtgatttgcg cggcagtcgc ggagagttct tgggaaagaa tttgagtaat gttcatcaat
     3781 agaattccaa cggacaggcc gtctgaaatt tcagacggcc tgatttaaaa acaatcgctt
     3841 taaggcagcg aattataata ttcgtaggct ttgtccatat cttcaaactg gtacatatgc
     3901 cctttttcca gcaccatcgc attatcgcaa tattgcttca tggcgctgtg gctgtgcgac
     3961 accaagatga tggaacggtc tttgcgcttt tcaaacaact cgtacttaca tttatcggca
     4021 aagcgcgagt caccaactgc aatcacttcg tcaatcaaat agcaatcaaa ctctaccgcc
     4081 aacgacaaag caaatgccaa acgcgccttc atacctgaag aataacgctt gaccggctcg
     4141 taaagatatt gccccaactc ggaaaactct tcggtaaagt tcttcacata atcgatatcg
     4201 acattgtaaa tccggcagat gaaacgcaaa ttgtccatac cggtcagact accttgaaac
     4261 gcaccggaga atgccaaagg ccaagaaata ctcattgtcc gcttgatttc acccgtggtc
     4321 ggcggctcaa cgccactgat caaacggatg agcgtcgatt tacctgcacc gttgcggccg
     4381 agaataccga ttttctcgcc cttctccatt ttgaagctaa tatcgtgcaa gactgtccgc
     4441 caaccttggc gggtcagata gcgtttggaa acgtgttcaa ctgaaatcat tgcggctcaa
     4501 cccctttgct gaatctcgcc accaccgcca aaccaagcag cagcaaaacc aagttacaca
     4561 acaaaatata ccaaggattt tcgtaagtcg tgacgctgtc gccaaaatat ccggcacgga
     4621 acatttctgt gccatgcacc atcgggatca tcaatgcata ctcttgcact ttcggaggca
     4681 aattatgtac aaaaaagaat gcacccgata acggcatcat cacaaaagtc aatgtgcccc
     4741 aaatcttgcc aaacggctcg aaattaaagg caatcgaaca aatcaccaaa cccaaaccaa
     4801 tcgcaaaaaa agccatcaaa agccaagcca tcagcatata aaacatatct gccggcattt
     4861 caatccagcc aatcgcaatc aataccgcca taatcacaat ctgcgcaatg gttgcaccag
     4921 caatttccaa aatcatgcgc gccaagatgg tatccaaaac tcttacattg cggtgataaa
     4981 gcaagctggc atttgaagaa atcgacccaa ctgcccgttt tgaggcatta cgccacatca
     5041 tcaacatcgg atagccagta atcgcaaatg cgacaatatt caaagttgaa tatcggtctg
     5101 cctttaaaaa tttccacatc aagacgataa cgaatgtcat cagcaacggc tcaacaaaca
     5161 gccataaaaa gccaatatta ttgcgaccgt aacgggtgat aatttcccgc atcaaaagcg
     5221 caccgattac gcgcctttga atggctaaag attcccaaaa tgatgtttta tgcaaggctt
     5281 tcatcagttt ttatgctcac gaatgctggc agtcaacagg ctcaaaatac cataaaccat
     5341 caagccgata atcagagtgg caacaatgtt gtataaccgt ttaggctcat gtgccaaatc
     5401 cggcaggctc ggttgcgaga tcacttccaa ataaagctgc tgacggtctg cttcaacctt
     5461 ggcactttcc aaagaagtca tggcggctgc caactgctgc tctgccaact ggttttccaa
     5521 atacacacgc tgatattcgg cagcctgatt agacaacgaa gaatgcccac cgcccgaaat
     5581 ggcacgtaac tgttggtcaa tttctttacg caagctctgc tcacgcgcct gcaaacccgg
     5641 aatctgcgga ttctccggag tgactgcttt cacctgatcc agctgggttt gaatcacaat
     5701 caattcatct tgcagcttgg aaaccaaccc catttgcact tccgattgcg ctttcaaatc
     5761 aaaaacgcca ttggcaatcc ggtaatccgt cagattctga gaggcttcct ttacccgctc
     5821 tgccgccgtt ttcactactt cttccgcata gcgcaccgta tcagcacgtg cacgatcgtt
     5881 caactggtta atcaatgctt caccttgttt taacaaagcc tcattgattt tcttagattc
     5941 cagcgcatca aaggaagtta cattcaacgt ggaaataccc gaaaccgtat caaaattgat
     6001 catcacctga tttttatagt attgataaaa agcctcttcc tcgccacgga atccaaaacc
     6061 attaaatcgg ctaaatacat cgcccttagt ttcataaaat tcacgcaccg gcaagatttt
     6121 acgcagttca tccaaagacg agcgcgaacg catatactcc ccaaccgtgt aaatatcatc
     6181 ttgcgcacgg gcaaaacctg tgccctgcaa aatggcaccc aggccattga gagaagattg
     6241 gcttttaggc gagcgcacca caaagctcga ttgcgacgta aaacgatcgg aagcgaagaa
     6301 gccgaaatac accaacgaaa ttaccgtagg gataatcacc gttacccaaa ataaagggct
     6361 tagcttttta atccaacttt tctttttcgg ctttttacgc tcggctttgg tttcagtggc
     6421 aactgccaca ggaagttgct cagacatctt aattactcac attaattagt taaattatta
     6481 atactgttcg cgccactggt aaccggcgag aacacaaacg acaagaattt ctgcacttca
     6541 gccaacggcg cattcgacac atacaataca tctttattct tcacaggaaa gcgctgcata
     6601 gaaaatagcg aatgcgcatc agccatattc acacgatata ccgttggaat ctctgcctca
     6661 ctgccataac cttgagcaat ccatttatcc tgacgttctg ccggcaattc caccaatggc
     6721 gtatagcgga acacaaacac accacgcgca tcagaacggc gatcttgcaa accgcccata
     6781 cggccaatgg cttcagaaag cgataagcct ctggctgaaa aaccgatttc ttgtgttctc
     6841 cccaccgcac ccatagacgt aaaggtatag ggattggtaa tcatggtaac cacatcaccg
     6901 cgacgcagca aaatattttg tcgcggattt gcaactaaat cttccaaggc aacagttcgt
     6961 actacattgc cacgtgtcag ctgcacattc gtatcctgca catttgccgt tgaaccacct
     7021 accgcagcca ccgcatccaa cacacgctca ccggctgccg tcagcggcat acgcacacta
     7081 ttcccagcac gaatcaccga cacattcgcc gcattattct gcaccaaacg caccatcact
     7141 tgtggctgat tggccatttt tttcaggcgg cctttaataa tttcctgaac ctgaccaggc
     7201 gttttaccga ccaccgaaat atcgccaaca aacggcacag aaaccgtacc acgtgccgtg
     7261 accaactgct ctggcaactt agtttgatgc gcactacccg agcccatcga agaaaggcca
     7321 ccaccaaaca atactgccgg cggcgcttcc caaatcataa tatccaatac atcaccaata
     7381 tttagcgtac cagccgaagc ataaccatcg ccaaactgag tgaatgactg atttatctga
     7441 gccttatata ataactgagc aaccgtatga ttcacatcaa tcagctccac ttcaggaatt
     7501 tgaacttcag attgttgccc taaagagaca attttttttg cgctggggcc tgatgaagga
     7561 atcgcagagc atcctacaat taaacttcca cacaataata atactgcgtg acgaatataa
     7621 aatttcactt taaacacaag ccaaatccta atataattat aaatggccta attatagcac
     7681 ttaatcgaaa taaatttatg agtacgtaga gtataattag tattcttctt tccaacttcc
     7741 ttatacttat atacttatag attctaaaat catgaaaaga attctttgca ttacaggtac
     7801 cagagccgac ttcggcaagc taaaaccttt attagcctat attgaaaatc acccagacct
     7861 tgaattgcat ttgattgtaa ctggtatgca tatgatgaaa acatatggca gaacctgcaa
     7921 ggaagtaact cgagaaaact atcaacatac atatctgttt tcaaatcaaa tccaaggtga
     7981 accaatgggt gccgttttag gcaataccat tacgtttatc tctcgtctat ctgatgaaat
     8041 tgaacctgat atggtcatga ttcacggcga ccgtttagaa gcactagcag gcgcaactgt
     8101 aggtgcatta agcagccgtt tagtttgcca tatcgaaggt ggtgaactat ctggtacagt
     8161 agatgactcc attcgtcatt ctattagtaa actttctcat atccacttgg tagcaaatga
     8221 acaagctgtc actcgcctag tgcaaatggg agaaaaaaga aagcatattc acatcatcgg
     8281 ctcccccgat ttagatgtta tggcctcttc caccctccca tccttagaag aagtcaaaga
     8341 atattacggt ttaccatacg aaaattatgg tatttctatg tttcaccccg tgactacaga
     8401 agcacattta atgccacaat atgcggccca atatttcaaa gcattagaat taagtggcca
     8461 aaatatcatt agcatttacc ctaacaacga cacgggtact gaaagtattt tacaagagtt
     8521 attaaaatgt caatctgaaa aatttatcgc atttccatct atacgatttg aatacttttt
     8581 agtcttattg aaacatgcta aatttatggt cggcaactca agtgcaggta ttagagaagc
     8641 tcctctctac ggtgtccctt caattgatgt tggtacacgc caaagtaacc gccatatggg
     8701 aaaatctatt attcatacag attatgaaac taaaaatatc tttgatgcga ttcaacaagc
     8761 atgcagttta ggcaaatttg aagcagatga tacctttaat ggcggagata ctcgcaccag
     8821 cacagaaaga tttgctgaag taatcaacaa tcctgaaacg tggaatgttt ctgctcaaaa
     8881 acgttttatc gatttgaatc tttaaattat ggaaaaacaa aatattgcgg ttatacttgc
     8941 gcgccaaaac tccaaaggat tgccattaaa aaatctccgg aaaatgaatg gcatatcatt
     9001 acttggtcat acaattaatg ctgctatatc atcaaagtgt tttgaccgca taattgtttc
     9061 gactgatggc gggttaattg cagaagaagc taaaaatttc ggtgtcgaag tcgtcctacg
     9121 ccctgcagag ctggcctccg atacagccag ctctatttca ggtgtaatac atgctttaga
     9181 aacaattggc agtaattccg gcacagtaac cctattacaa ccaaccagtc cattacgcac
     9241 aggggctcat attcgtgaag ctttttctct atttgatgag aaaataaaag gatccgttgt
     9301 ctctgcatgc ccaatggagc atcatccact aaaaaccctg cttcaaatca ataatggcga
     9361 atatgccccc atgcgccatc taagcgattt ggagcagcct cgccaacaat tacctcaggc
     9421 atttaggcct aatggtgcaa tttacattaa tgatactgct tcactaattg caaataattg
     9481 tttttttatc gccccaacca aactttatat tatgtctcat caagactcta tcgatattga
     9541 tactgagctt gatttacaac aggcagaaaa cattcttaat cacaaggaaa gctaaatgca
     9601 aaacaacaac gaatttaaaa ttggtaatcg ttcagtaggt tacaaccacg aaccattgat
     9661 tatctgtgaa atcggcatca atcatgaagg ctctttaaaa acagcttttg aaatggttga
     9721 tgctgcctat aatgcaggcg ctgaagttgt taaacatcaa acacacatcg ttgaagacga
     9781 aatgtctgat gaggccaaac aagtcattcc aggcaatgca gatgtctcta tttatgaaat
     9841 tatggaacgt tgcgccctga atgaagaaga tgagattaaa ttaaaagaat acgtagagag
     9901 taagggtatg atttttatca gtactccttt ctctcgtgca gctgctttac gattacaacg
     9961 tatggatatt ccagcatata aaatcggctc tggcgaatgt aataactacc cattaattaa
    10021 actggtggcc tcttttggta agcctattat tctctctacc ggcatgaatt ctattgaaag
    10081 catcaaaaag tcggtagaaa ttattcgaga agcaggggta ccttatgctt tgcttcactg
    10141 taccaacatc tacccaaccc cttacgaaga tgttcgattg ggtggtatga acgatttatc
    10201 tgaagccttt ccagacgcaa tcattggcct gtctgaccat accttagata actatgcttg
    10261 cttaggagca gtagctttag gcggttcgat tttagagcgt cactttactg accgcatgga
    10321 tcgcccaggt ccggatattg tatgctctat gaatccggat acttttaaag agctcaagca
    10381 aggcgctcat gctttaaaat tggcacgcgg cggcaaaaaa gacacgatta tcgcgggaga
    10441 aaagccaact aaagatttcg cctttgcatc tgtcgtagca gataaagaca ttaaaaaagg
    10501 agaactgttg tccggagata acctatgggt taaacgccca ggcaatggag acttcagcgt
    10561 caacgaatat gaaacattat ttggtaaggt cgctgcttgc aatattcgca aaggtgctca
    10621 aatcaaaaaa actgatattg aataatgctt attaacttag ttactttatt aacagaggat
    10681 tggctattac atatagctaa ttctcattaa tttttaagag atacaataat gctaaagaaa
    10741 ataaaaaaag ctctttttca gcctaaaaag ttttttcaag attcaatgtg gttgacaaca
    10801 tctccatttt atcttacccc cccacgtaac aatttatttg tcatatctaa tttaggtcag
    10861 cttaaccaag tccaaagcct aattaaaata caaaaattaa ccaataattt actagtaatt
    10921 ttatatactt ctaaaaactt aaaaatgcct aagttagttc atcaatcagc taacaagaat
    10981 ctatttgaat ctatttatct atttgagctt cctagaagcc ctaataatat aactcctaaa
    11041 aaattacttt atatttatag aagttacaaa aaaatcctta atattataca gcctgctcat
    11101 ctctatatgc tgtcttttac aggccactac tcctatctga ttagtattgc aaaaaaaaag
    11161 aatattacga ctcatttaat tgatgaaggg actggaacat atgctccttt attagaatca
    11221 ttttcatatc atccaacaaa attagaacgt tatttgattg gaaataatct taatattaaa
    11281 ggatatatag atcattttga catattgcat gtcccctttc ctgaatatgc taaaaaaata
    11341 tttaatgcaa aaaaatataa ccggtttttt gcgcatgctg gaggaataag cattaataat
    11401 aacattgcaa acttacagaa aaaatatcaa atatctaaaa atgactatat ttttgttagt
    11461 caacgctacc ccatttcaga tgatttgtat tataagagta tagtagaaat cttaaacagc
    11521 ataagtttac aaattaaagg aaagatattt attaaactac acccaaaaga gatgggcaac
    11581 aactatgtaa tgtctttatt tctaaatatg gtagaaataa accctcggct ggtagttatt
    11641 aatgaacctc cttttctaat tgagccccta atatacttaa caaatcctaa aggaattata
    11701 ggcctggcct ctagttcttt aatttataca ccattactct caccctcaac ccaatgtctt
    11761 tctattggag agttaattat taacttaatt caaaaatatt caatggtgga aaacactgaa
    11821 atgatccaag aacacttaga gattattaag aaatttaatt ttattaatat actaaatgat
    11881 ttaaatgggg taataagtaa ccctctcttt aaaacagaag aaacatttga aacacttctt
    11941 aaatctgcag aattcgcata taaatctaaa aactactttc aggctatttt ttactggcaa
    12001 cttgccagca aaaacaatat taccttatta gggcataaag cattatggta ctacaatgca
    12061 ctttataatg taaaacaaat ttataagatg gaatattcag atatttttta tatcgataat
    12121 atctccgtag actttcatag taaagataaa ttgacatggg aaaaaattaa acattattac
    12181 tattccgccg acaatagaat tggtagagat agataaaaat actatgactt ctgatctcca
    12241 tacgtatgct tgttttcaaa aagcagcatt aagatagata agtgaagctt gatgagagcc
    12301 atttttgtgg tcatcgccaa ggtacacgcg gagagtaatc gacaacagag ctgctgtatt
    12361 ctggctttta aagcccagta gtaaagttta taaggctgca tttctcaatg taaaacgcga
    12421 tattttcacc cattattagg gataagtagc cagtggttta cactggctac tttcatagtt
    12481 atgaagtact gggtctgagt gaattcaatt atttccgtat aaaacacagt agacatttta
    12541 ctaatcggca aaattatatt agaagaattg agaactactg aagtcaggta agataccatt
    12601 ttgcacaaat ttaacggcat ccctaaaaag cattttgggt ggtatttaaa ggagtacaaa
    12661 atggttttaa ctacagtatg cttagaaatc tatagcctta cgcgctttat attaaatcac
    12721 ctttctcaac catttagaat cataaaaaaa ctttttagga tccctaatta actttttaaa
    12781 ttttcttaaa aaaatagttt tagttgattc tatactattc accgcagata aatattcttg
    12841 gtaatcatgt tgcaatggca caacctgctt taatagctta ttaaactcat cagttgtaat
    12901 caccttttta gtagcatagc aatcaagcca atccggatct gctatttttg gggaagtgaa
    12961 atctatttga tcaaaataaa ctaaaacccc actacctaaa aaatgctgat ttgcatagga
    13021 tttagaaaag cctaagtctg caacaacacc aacagggata cccttacatt cagcttcaaa
    13081 aagcatagta gatgaataag ataaacaata ccccatttct tggaaggctt gcgcggtact
    13141 ttttcgctct attgtcaaat tactcggcaa ctgaaactgc tttgccagct ctatatacga
    13201 atgtttatcc tgatgcacag taatatcttt atctgcaacc cttagcaaaa tagtaaattc
    13261 tttctcaggg tatttttcag ccaaggcaat caattttttt aatgtataaa ttctttcttc
    13321 ttttttaaat gggattttaa cttggtcaat aaagtaaatt ttctctccat gacgaccttt
    13381 tgatgcgtgg cgaaaaattg gataaccata aagtatattt tgacaactta atttatattg
    13441 tttcttatat tcctctgcaa ttctaaaatc atgcttatta ttatataaaa caatatcagc
    13501 ccccatacga gatagaatac ttgcctgatc accgaatact acacctggaa acaaggtaat
    13561 aatcaatggc cttgaagcaa tatttaattg cgcattctgc ttaaagaacc tttttaacaa
    13621 cccatttcca accgataaaa ttactgcgtc ataatattga tgcatattct taataaagta
    13681 atcatcaata tgaaaaaaaa aattgcttgg tctttatcta tccctgattc taataactgt
    13741 cgatttgaaa gaatgttttc tcgacttttg tggatataaa tatcaatttg agcatctttt
    13801 atctcttttg caacagcata gcccgagtta aggaacgagt cataactggc aattagtaat
    13861 acttttttca ctattttccc ttaattctac accatatgaa taaaattata gtattttacc
    13921 ttagatatca aatatactta accaaaaaaa tctgaagaaa tctactgcta cttcagggat
    13981 aattatttca ttaagatttt acaaccatta ttatcaatct atcaaagtat ttttagtatt
    14041 ttattctatg aaaaaaattc tcgttaccgg cggcaccggt tttatcggct cgcataccgt
    14101 tgtttctttg ctgaaaagcg gccatcaagt cgtgattttg gataacctat gcaattccag
    14161 catcaatatc ctgccacgct tgaaaacgat taccggccaa gagattccgt tttatcaagg
    14221 cgatatccgc gatcgtgaga ttttgcgccg tatttttgcg gaaaaccgca ttgattcggt
    14281 gattcatttt gccggcttga aagcggtggg tgaaagtgtg gccgagccga tgaaatatta
    14341 tgataataat gtttccggca gcttggtgtt ggcggaagaa atggcgcgtg cgggcgtgtt
    14401 tagcattgtg ttcagttctt cggcgacggt ttatggcgat ccgggcaaag tgccttatac
    14461 cgaggatatg ccactgggcg ataccaccag cccttacggc
//
LOCUS       DAWFHY010000052        14215 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.052, whole genome shotgun sequence.
ACCESSION   DAWFHY010000052 DAWFHY010000000
VERSION     DAWFHY010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 14215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.052"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(69..2045)
                     /locus_tag="WGQ68_001360"
     CDS             complement(69..2045)
                     /locus_tag="WGQ68_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEZ0715144.1"
                     /translation="MNEFSLAPIVVVLLVSVITVILCRKFNIPSMLGYLLVGFLAGPG
                     MLSLIPKSHATDYLGEIGIVFLMFSIGLEFSLPKLRAMRRLVFGLGGLQVGVTMLSVM
                     GILMLTGVPFNWAFAVSGALAMSSTAIVSRILSEKTELGQPHGQMAMGVLLMQDIAVV
                     PLMILIPALAGGGDGNIWAALGLAFAKMLLTLGLLFFVGSKIMSRWFRMVAKRKSSEL
                     FMINVLLVTLGVAYLTELEGLSMALGAFVAGMLLSETEYRFQVEDDIRPFRDILLGFF
                     FITVGMKLDIQALIGGWRQVLMLLAMLLVLKALVVFAIAFKMKHSVGDSLKTALYLAQ
                     GGEFGFVMLAIAGQLDMVSPEWEQAATAAVLLSMIIAPFLLGGSDALVGRLVKSSWDM
                     KSLDLHSMLVETMSKSDHVLIVGFGRGGQTVGRVLAQEDIPYFALDLDIARVQVARSA
                     GEPVSFGDAKRREVLEAAGLGRAKMVVVTLNNMHETQHVLDNVLSMYPNMPVYVRATN
                     DDYVKTFTDIGAEEAVSDTKETGLVLAGYAMLGNGASYRHVYQTMANIRHSRYAALEG
                     LFVGSDDEAGFGETGETVRHAFPLAAEAYAVGKTVGTLPMAAYGIKLLFVRRRTGRIE
                     NPDASFTLEGGDVLVVAGKKEEIISFENWSLQGI"
     gene            2194..3033
                     /locus_tag="WGQ68_001361"
     CDS             2194..3033
                     /locus_tag="WGQ68_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HEZ0715145.1"
                     /translation="MTATAAAIDRLLPQTQCRECGYDGCLPYAQAVATGEAYNLCAPG
                     GEAVIRDISALLGKPLVAPAKTQAKALARIDETACIGCTACIRVCPADAIMGAGKLMH
                     TVIADECTGCGLCVAPCPVDCIHMQPVADTVLPRARRFSLSADSRFAAAEHARARYLK
                     RNERKQREADERKAMLAEREAAVRNARPQTPDTPKKPAFNPADLIAKAMAKAQTQQDR
                     LAAADNRKDYQAKQIAEARERAELRRAQRDMKYGSDSEKAAALEYLKQYKAKQEAAQN
                     TAS"
     gene            3234..4265
                     /gene="gap"
                     /locus_tag="WGQ68_001362"
     CDS             3234..4265
                     /gene="gap"
                     /locus_tag="WGQ68_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEZ0715146.1"
                     /translation="MGIKVAINGYGRIGRQVLRAIYDYQIQDQLQIVAVNASGSLETN
                     AHLTKFDTVHGRFEADVSHDGGNLIVNGDKIPFFSTRNPAELPWKELGVDLVMECTGA
                     FTSKEKAKIHLESGAKKVLISAPGGDDVDATVVYGVNDSILTADMTVVSNASCTTNCL
                     SPVAKVLSESVGIVKGAMTTIHALTNDQTVTDVRHKDLRRARSGVENMIPTKTGAAKA
                     VGLVLPELKGRLDGLAIRVPTVNVSLVDLSFQAARDTTIEEINALMKAASEAGALKGV
                     LGYNTLPLVSMDFNHTTEASHFDATLTKVVDGNMVKVFAWYDNEWGFSCQMLNTARRM
                     FGLEVRPLK"
     gene            complement(4409..5134)
                     /locus_tag="WGQ68_001363"
     CDS             complement(4409..5134)
                     /locus_tag="WGQ68_001363"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEZ0715147.1"
                     /translation="MRIPLLAPDNYAFPDPAYALAWCDGLVGVSGDLDAGRLLEAYRN
                     GVFPWFSRDGWFFWYAVGPRAVIVPERLHVPRSLAKTLRNGSYRVAVNGCFAEVVAHC
                     AAAARPNQDGTWIAPEFQTAYLKLHEMGHAHSFECHYPDESGETRLAGGFYGVQIGRV
                     FYGESMFALQPDASKIAFACAVPFLADLGVELIDCQQDTEHMRRFGSELLPFADFAER
                     LRMLNAVPLKEEIGRREVVCKGL"
     gene            complement(5204..5638)
                     /gene="fur"
                     /locus_tag="WGQ68_001364"
     CDS             complement(5204..5638)
                     /gene="fur"
                     /locus_tag="WGQ68_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HEZ0715148.1"
                     /translation="MEKFNNIAQLKDSGLKVTGPRLKILDLFETHAEEHLSAEDVYRI
                     LLEEGVEIGVATIYRVLTQFEQAGILQRHHFETGKAVYELDKGDHHDHIVCVKCGEVT
                     EFHNPEIEALQDKIAEENGYRIVDHALYMYGVCSDCQAKGKR"
     gene            5857..6234
                     /locus_tag="WGQ68_001365"
     CDS             5857..6234
                     /locus_tag="WGQ68_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEZ0715149.1"
                     /translation="MNKTLILALSALLGLAACSVERVSLFPSYKLKIIQGNELEPRAV
                     ASLRPGMAKDQVLLLLGSPILRDAFHTDRWDYTFNTSRNGIIKDRSNLTVYFENGVLV
                     RTEGNALQNAAEALRVKQNADKQ"
     gene            6244..7053
                     /gene="dapB"
                     /locus_tag="WGQ68_001366"
     CDS             6244..7053
                     /gene="dapB"
                     /locus_tag="WGQ68_001366"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEZ0715150.1"
                     /translation="MTPLKIAIAGANGRMGRVLVEAVNNHPDTVLSGALEHSGSEALG
                     LDAGYAVGLKTGIAISDDVDAVLAQSDVLIDFTRPEPTLKHLQKCVEKQVNIIIGTTG
                     FDDAGKAAIHTAAEQTGIVFAANFSVGVNLTFHILDTVARVLNEGYDIEIIEGHHRHK
                     VDAPSGTALRMGEVIAGALGRDLKQCAVYGREGHTGPRDPSTIGFATVRAGDIVGDHT
                     ALFATDGERVEITHKAGSRMTFAAGAVRAAVWVNGKTGLYDMQDVLGLNNR"
     gene            complement(7317..7727)
                     /locus_tag="WGQ68_001367"
     CDS             complement(7317..7727)
                     /locus_tag="WGQ68_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715151.1"
                     /translation="MTTYTIPKKDYQFLYIYEGTLLNYTLKNDEFHIIVQNVDYPDFP
                     QEIPTPNYTDWVKIKFKQFSYLKFIYGYATKSQDINIDNTLELGELKQDDEILDYGGA
                     LEVIGSRYDLPTGFSIDIVCREIELEFLDQESFN"
     gene            7950..8339
                     /locus_tag="WGQ68_001368"
     CDS             7950..8339
                     /locus_tag="WGQ68_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0715152.1"
                     /translation="MNVKINYNLLEYCEYLGGIFGVYKNYLNMDIKDPNLKIRFFDFL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRRIWPDMEEMLLYFPDNPWFYVEHF
                     WWGATCPIELTQLPKIEIYEEQMKQGK"
     gene            complement(8383..8541)
                     /locus_tag="WGQ68_001369"
     CDS             complement(8383..8541)
                     /locus_tag="WGQ68_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715153.1"
                     /translation="MDYFPEAARITKKGGEIVINGTSNNKYLRGIPNETELARMGLRL
                     KYNGQLTD"
     gene            complement(8549..9721)
                     /gene="lpxB"
                     /locus_tag="WGQ68_001370"
     CDS             complement(8549..9721)
                     /gene="lpxB"
                     /locus_tag="WGQ68_001370"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEZ0715154.1"
                     /translation="MWGQTNMVDKKSPLIAVSVGEASGDLLGAHLIRAIRKRCPQARF
                     TGIGGELMKAEGFESLYDQERLAVRGFVEVVRRLPEILRIRRGLVRDLLSLKPDVFVG
                     IDAPDFNLGVAEKLKRAGIPTVHYVSPSVWAWRRERVGKIVHQVNRVLCLFPMEPQLY
                     LDAGGRAEFVGHSMAQLMPLEDDRETARQTLGVDAGIPVFALLPGSRVSEIDYMAPVF
                     FQTALLLLKRYPAARFLLPAATEATKRRLAEILQRSEFAGLPLTVTDRQSETVCRAAD
                     AVLVTSGTATLEVALCKRPMVISYKISPLTYAYVKRKIKVPHVGLPNILLGKEAVPEL
                     LQHDAVPEKLAAALADWYEHPDKVAALQQDFRALHLLLKKDTADLAARAVLEEAGC"
     gene            complement(9769..10761)
                     /locus_tag="WGQ68_001371"
     CDS             complement(9769..10761)
                     /locus_tag="WGQ68_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEZ0715155.1"
                     /translation="MKTHEISKDSVSLIGVAEHEAGQRLDNYLIKILKGVPKSHIHRI
                     IRAGEVRLNKKRCKPDSRIAEGDTVRIPPVRVAEKEMPSERRAAVPARAFDVVYEDDA
                     LLVIDKPSGVAVHGGSGVSFGVIEQLRRARPEAKYLELVHRLDKDTSGLLMVAKKRSA
                     LVKLHEAIRNDHPKKIYLALGVGKLPDDNFHVKLPLFKYTGAQGEKMVRVSADGQSAH
                     TVFRVLSRFSDGILHGVGLSHLTLVRATLKTGRTHQIRVHLQSQGCPIAGDERYGDYQ
                     ANRRLQKLGLKRMFLHASELHLNHPLTGEPLVLKAELPLDLAQFAVMLENGTKM"
     gene            11255..14014
                     /locus_tag="WGQ68_001372"
     CDS             11255..14014
                     /locus_tag="WGQ68_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEZ0715156.1"
                     /translation="MKRMLFNATQAEELRVAIVDGQNLLDLDIETLGKEQRKGNIYKG
                     IITRIEPSLEACFVDYGTDRHGFLPFKEVSRSYFQDYKGGRARIQDVLKEGMEVIVQV
                     EKDERGNKGAALTTFISLAGRYLVLMPNNPRGGGVSRRIEGEERQELKAAMAELDIPN
                     GMSIIARTAGIGRSAEELEWDLNYLKQLWQAIEEAGKAHHDPYLLFMESSLLIRAIRD
                     YFRPDIGEILVDNQEVYDQVAEFMSYVMPGNIGRLKLYEDHTPLFSRFQIEHQIESAF
                     SRSVSLPSGGAIVIDHTEALVSIDVNSARATRGADIEDTAFKTNMEAAEEVARQMRLR
                     DLGGLVVIDFIDMENPKHQRDVENVLRDALKKDRARVQMGKLSRFGLLELSRQRLKPA
                     LGESSHVACPRCAGTGVIRGIESTALHVLRIIQEEAMKDNTGEVRAQVPVDVATFLLN
                     EKRAELFAMEERLDVNVVLIPNIHLENPHYEINRIRTDDVEEDGEPSYKRVAEPEEDE
                     SAKPFGGEKAKAARPEPAVKGVRHTSPAPTAAPEKKTSWWDSFKAWLKRIFGGSETQA
                     APAAETSEKRSTANRSGSRANNRRQNPRRSKREGSKVEVREVAGKTARQEARADTAET
                     RNNGNRRRNERGDRAAERANEAEIQSRNVQPAATVADAAPSETEVQTGKRRRNGSRNE
                     RGQTAPETAAVAETTVQTAENTPSEPHTAEDKGSKPKSERNRRERDSRDAKERRERNN
                     QRDRRQNGKKRNIPSAAKIEQYLNIHDTADKVRSAAAHVFGETDANAPITVSIADPVA
                     ERDLPTASPAVSNGDAPVYDAAEKIRRATAAILPEGATPKAEAQEMPSENATFTAAAE
                     QARETAQTGGLVLIETDPAALKAWAAQPEVQAGRGLRRSEQPKPSEAATVPAEEMIQV
                     ETRQG"
BASE COUNT         3642 a         4494 c         3313 g         2766 t
ORIGIN      
        1 tgcgatttgc gaaagcgtta ttaaaccaaa taaccggaaa tggcgcaagc cttatttcgg
       61 catttcattt atattccctg caaactccag ttttcaaaag agataatttc ttcttttttg
      121 cctgcgacca ccaacacgtc accgccttcc aatgtaaacg aggcatccgg gttttcaatc
      181 cggccggtgc ggcggcggac gaacaagagt ttgatgccgt aagccgccat cggaagcgtg
      241 ccgactgttt tgccgacggc gtatgcttct gcagccaaag gaaaggcgtg gcggacggtt
      301 tcgccggttt cgccgaatcc tgcctcatca tcactaccga caaacagtcc ctccaacgcg
      361 gcataacggc tgtggcggat atttgccatc gtctgataga cgtgccgata cgacgcgccg
      421 ttgcctaaca tcgcatagcc tgccagcacg agtccggttt ctttggtgtc cgacacggct
      481 tcttccgcac ctatatcggt aaacgttttc acataatcgt cgttggtggc gcgcacatat
      541 acgggcatat tgggatacat ggacagcaca ttgtctaaaa cgtgttgcgt ttcgtgcata
      601 ttgttgagcg taaccaccac cattttcgcc cgtcccagac cggcggcttc caatacttcc
      661 ctgcgtttcg catcgccgaa cgacaccggt tcgcccgcac ttctggcaac ctgcacccgc
      721 gcaatgtcca agtcaagcgc gaaatacgga atatcctctt gggcaaggac gcgtccgacc
      781 gtctgcccgc ccctgccgaa gccgacaatc agcacatggt cggacttgct catggtttct
      841 accagcatac tgtgcagatc gagcgacttc atgtcccagc ttgacttgac caaacgcccg
      901 accagcgcat cgctgccgcc caagaggaag ggcgcgataa tcatcgacag cagaaccgcc
      961 gccgtcgccg cctgttccca ttctggcgaa accatatcaa gctgcccggc aatggccagc
     1021 atcacgaagc cgaactcgcc gccctgcgcg agatacaaag ccgttttgag gctgtcgccg
     1081 accgaatgtt tcattttgaa ggcaatggca aacacaacca gtgccttcaa caccagcagc
     1141 attgccaaca gcatcaatac ctgccgccag ccgccgatca atgcctgaat gtccagcttc
     1201 ataccgaccg tgataaagaa aaagccgagc aaaatatcgc ggaacgggcg gatgtcgtct
     1261 tcgacttgga aacggtattc cgtttccgaa agcagcatcc ccgccacaaa cgcgcccaac
     1321 gccatagaca aaccttccag ctcagtcaga taagccacac ccaaggttac cagcagcaca
     1381 ttgatcataa agagttcgga cgatttgcgt tttgccacca tcctgaacca tcgcgacata
     1441 attttgctgc cgacgaaaaa cagcagcccc agcgtcagca gcatttttgc aaacgccaaa
     1501 cccaaggccg cccaaatatt tccgtcccct ccgcccgcca gcgcgggaat cagaatcatc
     1561 agcggcacga cggcgatgtc ctgcatcagc agcacgccca tcgccatctg accgtgcggc
     1621 tgccccaatt ccgtcttttc cgacaaaatc cggctcacaa tcgccgtgga cgacatcgcc
     1681 aacgcgcccg acacggcaaa cgcccaattg aacggcacgc ccgtcagcat cagtatgccc
     1741 attaccgaca gcatcgtaac gccgacctgc aaaccgccca gcccgaacac cagccgcctc
     1801 atcgccctca acttgggcag cgagaactcc aaaccgatgc tgaacatcag gaacacaatc
     1861 ccgatttcgc ccaaataatc cgtcgcatgg cttttcggaa tcaggctgag cataccgggc
     1921 cccgccaaaa agcccaccag caggtagccc agcatggagg gaatgttgaa cttgcggcac
     1981 aggatcaccg taatgaccga caccagcaaa acaaccacaa taggggcaag cgaaaattcg
     2041 ttcataggcc gtccgaacag gaaaatacag aaaaatgccg tctgaaacgg catacgccgc
     2101 cgtattataa caaaacaccg cgcaccatcc gaaacgggcg cggcatacaa ttctgctaaa
     2161 atacgccctt tcgattttga gccgcacacc gacatgaccg ccacagccgc cgccatagac
     2221 cgcctgcttc cccaaaccca atgccgcgaa tgcggctacg acggctgcct gccctacgca
     2281 caggcagtcg caacaggcga agcgtacaac ctctgcgccc cgggcggaga agccgtcatt
     2341 cgggacattt ccgccctgct cggcaaaccc cttgtcgcac ctgccaaaac ccaagccaaa
     2401 gcactcgccc ggatagacga aaccgcctgt atcggctgca ccgcctgcat ccgcgtctgc
     2461 cctgccgatg ccattatggg cgcgggcaaa cttatgcaca ccgtcatcgc cgacgaatgc
     2521 accggctgcg gactctgcgt cgccccctgc cccgtcgact gcatccatat gcagcccgtt
     2581 gccgacaccg tcctgccccg cgcgcgccgc ttcagcctgt ccgccgacag ccgttttgcc
     2641 gccgccgaac acgcgcgcgc gcgctacctc aaacgcaacg aacgcaaaca gcgcgaagcc
     2701 gacgaacgca aggccatgct tgccgaacgc gaagccgccg tccgtaacgc gcgtccgcaa
     2761 acgcccgaca caccgaaaaa accggcgttt aaccctgccg acctcatcgc caaagccatg
     2821 gcaaaagcgc aaacccaaca agaccgcctc gccgccgccg acaaccgcaa agactatcag
     2881 gcgaaacaga tagccgaagc ccgcgaacgc gccgagttgc gccgcgccca acgcgatatg
     2941 aaatacggca gcgacagcga aaaagccgcc gccctcgaat acctcaaaca atacaaagcc
     3001 aaacaggaag ccgcacaaaa caccgcctcc tgacccttcc ctgatatgcc gtctgaaaac
     3061 cgtttcagac ggcattttat caagctctcc gtccgccgca cccgtgccgt ccgcatctta
     3121 ccgcccaccc ttccggccgc gctgttttca ataaaatatt aattacacgc cactacaaat
     3181 ttgctataat ccgccccgaa aatctaccca acccctcaac aaaggaacaa accatgggca
     3241 ttaaagtcgc catcaacggc tacggacgca tcggccgcca ggttttgcgc gccatctacg
     3301 attatcagat tcaagaccaa ctccaaatcg tcgccgtcaa cgccagcggc agccttgaaa
     3361 ccaacgccca cctcaccaaa ttcgacaccg tgcacggacg ctttgaagcc gacgtatccc
     3421 acgacggcgg caacctcatc gtcaacggcg acaaaatccc cttcttctcg acccgcaacc
     3481 ctgccgaact gccgtggaaa gaactcggtg tcgatttggt catggaatgc accggcgcgt
     3541 tcaccagcaa agaaaaagcc aaaatccacc tcgaaagcgg tgcgaaaaaa gtcctcattt
     3601 ccgcaccggg cggcgacgat gtcgatgcaa ccgtcgtgta cggcgtgaac gacagcatcc
     3661 taaccgccga catgaccgtc gtttccaacg cttcctgcac caccaactgc ctctcgccgg
     3721 ttgccaaagt gttgagcgaa agcgtcggca tcgtcaaagg cgcgatgacc accatccacg
     3781 cgctgaccaa cgaccaaacc gttaccgacg tgcgccacaa agacctgcgc cgcgcccgca
     3841 gcggcgtgga aaacatgatt ccgaccaaaa ccggcgcggc aaaagccgtc ggactggtac
     3901 tgcccgaatt gaaaggcaga ctcgacgggc ttgccatccg cgtgccgacc gtcaacgtat
     3961 cattggtaga tttgagcttc caagccgcgc gcgacacgac aatcgaagaa atcaacgcac
     4021 tgatgaaagc cgcctcggaa gcaggcgcgc tcaaaggcgt tttaggctac aacaccctgc
     4081 ccttggtttc catggacttc aaccacacca ccgaagccag ccacttcgac gcaacgctga
     4141 ccaaagttgt tgacggcaac atggtcaaag tgttcgcttg gtatgacaac gaatggggct
     4201 tcagctgcca aatgctgaac accgcacgcc gtatgttcgg acttgaagtg cgcccactca
     4261 aataagcaac aaaccgtcaa acaaaatgcc gtctgaaacc cgatgttttc aagttccaga
     4321 cggcattttt caccgtgatt ttacccggct gtcgtcattt ccaattttaa tccgccatat
     4381 ttgaacctga ccgaagcata agccgccatc aaagcccctt gcacaccact tcgcgccgcc
     4441 cgatttcctc tttcaacggc acggcgttca acatccgcag acgttcggca aaatccgcaa
     4501 acggcagcag ctccgaaccg aaacggcgca tatgttccgt gtcctgctgg cagtctatca
     4561 gttccacacc caaatccgcc aaaaacggca cggcgcaggc aaacgcgatt ttcgacgcat
     4621 ccggttgtaa tgcgaacatc gattcgccgt aaaacaccct gccgatttgc acgccgtaaa
     4681 agccgcccgc caacctcgtt tcaccgcttt catcgggata atggcactcg aaagaatgcg
     4741 cgtgtcccat ttcgtgcagc ttcaaatatg ccgtctgaaa ctcgggcgca atccaagttc
     4801 cgtcctgatt cgggcgcgcc gctgccgcac aatgcgcgac cacttccgca aaacagccgt
     4861 tgaccgcaac ccgatagctg ccgttgcgca gcgttttcgc cagcgagcgc ggaacgtgca
     4921 gacgttcggg aacaatcacc gcacgcgggc cgaccgcata ccaaaaaaac caaccgtccc
     4981 gagaaaacca cggaaacacg ccgttccgat acgcctcgag cagccgcccc gcatccaaat
     5041 cgccgctcac gccgaccagc ccgtcgcacc aggccaaagc ataggcagga tcgggaaagg
     5101 cataattgtc aggggcaagc agcggaatac gcatggcagt tctccaaaaa cacagacggc
     5161 atccaaaatc atgaatgccg tctgaacaac aaaccgtccg gatttaacgt ttgcccttgg
     5221 cctgacagtc gctgcacacg ccgtacatat aaagcgcgtg atcgacgatg cggtagccgt
     5281 tttcctcagc gattttgtct tgcagggctt cgatttcggg attgtggaat tccgttacct
     5341 cgccgcactt cacgcagacg atatggtcgt ggtggtcgcc cttgtccaac tcataaaccg
     5401 ccttgcccgt ttcaaaatgg tggcgttgca gaatgcccgc ctgctcaaac tgggtcagca
     5461 cgcggtaaat cgtcgccaca ccgatttcca caccctcttc caacaaaatg cggtacacat
     5521 cttccgcact caaatgctct tccgcatgcg tctcgaacaa atccaaaatc ttcaaacgcg
     5581 ggccggtaac cttcagaccg ctgtctttca gttgtgcaat attgttgaat ttttccataa
     5641 tattcaatac ccctgtaaaa caatagacgt tataatacgc aatttcggcc tgtttgccca
     5701 ctatcgcacc atagcagttt gcaatagtaa aaccgcaccg gcggccgatg cgcggacagg
     5761 gcggataccg cttaatcgta tgattatcgt tcgcttttat aaaatattca agcagtgtta
     5821 cactacccat cccgatttgc acagaaaggc atttccgtga acaaaaccct catcctcgcc
     5881 ctttccgccc tcctcggcct tgccgcgtgc agcgtcgaac gcgtttcact gttcccctcg
     5941 tacaaactca aaatcataca gggcaacgaa ctcgaacctc gcgccgtcgc ctccctccgc
     6001 cccggtatgg ccaaagacca agtcctgctc ctgctcggca gccccatact gcgcgacgca
     6061 ttccataccg accgctggga ctataccttc aacacctccc gcaacggcat catcaaagac
     6121 cgaagcaatc tgaccgtcta ttttgaaaac ggcgtgctcg tccgcaccga aggcaacgcc
     6181 ctgcaaaatg ccgccgaagc cctccgcgta aaacaaaacg cagacaaaca ataaggaaca
     6241 cacatgacac cgctcaaaat cgccatcgcc ggcgcaaacg gccgtatggg gcgcgtcctc
     6301 gtcgaagccg tcaacaacca tcccgacacc gtcctttccg gtgcgcttga acactcaggc
     6361 tcagaagccc tcgggctgga cgcaggctac gccgtcggac tcaaaaccgg catcgccatt
     6421 tcagacgacg ttgacgccgt tctcgcacaa agcgacgtac tcatcgactt cacccgcccc
     6481 gagcccaccc tcaaacacct gcaaaaatgc gttgaaaaac aagtcaacat catcatcggc
     6541 acaacaggct tcgacgatgc gggcaaagcc gctatccaca ctgccgccga acaaacaggc
     6601 atcgttttcg ccgccaactt cagcgtcggc gtcaacctca ccttccacat cctcgacacc
     6661 gtcgcccgcg tgttaaacga aggctacgac atcgaaatca ttgaaggcca ccaccgccac
     6721 aaagtcgatg cccccagcgg caccgcgtta cgcatgggcg aagtcatcgc cggcgcgctc
     6781 ggcagagacc tcaaacaatg cgccgtttac ggccgcgaag gccacaccgg tccgcgcgat
     6841 ccgtcgacca tcggctttgc caccgtccgc gcaggcgaca tcgtcggcga ccacaccgcc
     6901 ctcttcgcca ccgacggcga gcgcgtggaa atcacccaca aagccggcag ccgcatgacc
     6961 tttgccgccg gcgccgtccg cgccgcagtt tgggtaaacg gcaaaacggg tttgtacgat
     7021 atgcaggacg tactcgggct gaacaaccgt taacccccat acaaaatgcc gtctgaaaag
     7081 atattgttca gacggcattt tgccgacagg ctccgtatcg gcatatcaat gtttcaacac
     7141 acaggacgac acataaagcg tcgccctatg tgttgccctg attcggaagg gattacgccc
     7201 ctcccaaata aagtctgatt ctaccgcccc gaaggacaga tgtccgagtg gcggggtttc
     7261 aaccgaaaag gaaatacgat aaagttgctt gcgcggcatg acaagttacg gctcgtttaa
     7321 ttaaaactct cctgatctaa aaattctaac tctatttccc ggcaaactat atctatacta
     7381 aaaccggtcg gaagatcata cctactgcct atcacttcca gcgcacctcc ataatccaag
     7441 atttcatcat cctgctttaa ttctccaagt tccaatgtat tatcgatatt tatatcttgg
     7501 ctctttgtag cgtatccata gataaatttc agatagctga actgcttgaa tttaattttt
     7561 acccagtctg tataatttgg tgtaggaatc tcttgaggaa agtccggata atccacattc
     7621 tggacgatga tatggaattc atcgtttttc aaagtatagt ttaatagagt gccctcatat
     7681 atatacagaa attgataatc tttttttgga atggtataag ttgtcatatt attttacctt
     7741 ttcaaaaaca atgagtttaa gattttttga gtccaagttg gttctactac tcttatgaaa
     7801 tttcagttgt ttaaactctt cctttatttt aaggaatttt tcaactagcc gagaacaaac
     7861 tggagcaaaa tggacgattg agtttaggaa tcaacctgat tcagtacatg ggcaaagatt
     7921 agaattaaaa tttagataaa ggttacgtta tgaatgtgaa gataaactat aatcttttag
     7981 aatattgtga atatttaggt ggtatttttg gtgtttataa aaattattta aatatggata
     8041 tcaaagatcc aaatttaaaa attagatttt ttgatttttt ggaagaactt ttatcagatg
     8101 gtgttattga attatgcgat tatcgtgaaa atcctccaaa aatattaact ggttcgccaa
     8161 aaacacaggt agatgaatta agaagaattt ggccggatat ggaagagatg ttgctatatt
     8221 ttccagataa tccttggttt tatgtagaac atttttggtg gggagcaact tgccctattg
     8281 aactgacaca attgcctaaa atagaaattt acgaagaaca aatgaaacaa ggtaagtaag
     8341 cgtattagta taagctataa tcataaatca tatataacaa aattaatcag ttaactgacc
     8401 attatatttt aaccttaatc ccattcgggc cagttctgtt tcatttggaa ttcctcttaa
     8461 atacttattg tttgatgtgc cattaatcac aatttctccc cctttttttg taatacgtgc
     8521 tgcttcggga aaataatcca ttaaccgctc aacatcccgc ctcttccaaa accgcgcgcg
     8581 cggccaaatc cgccgtatct tttttcaaca gcaggtgcaa cgccctgaaa tcctgttgca
     8641 gcgcggcaac cttatcgggg tgttcgtacc agtccgccaa cgccgccgcc agtttttccg
     8701 gtacggcatc atgttgaagc agctccggca cagcctcctt acccaacagg atattcggca
     8761 ggccaacatg cggcactttg attttgcgtt tcacataagc ataagtcagc ggcgaaatct
     8821 tgtagctgat gaccatcgga cgcttgcaca gcgccacttc caaagtcgcc gtaccgctcg
     8881 ttaccagcac cgcatccgcc gccctgcaca ccgtttcaga ctgtctgtcg gttaccgtca
     8941 gcggtaatcc ggcaaactcc gaacgctgca aaatttccgc caaacgccgc ttcgtcgcct
     9001 ccgttgcggc aggcagcagg aagcgtgcgg cgggatagcg tttcaacaac aataatgccg
     9061 tctgaaaaaa caccggcgcc atatagtcga tttcgctgac gcggctgccg ggcagcaggg
     9121 cgaatacggg gatgccggca tccacgccca aagtttgccg cgccgtttca cggtcgtctt
     9181 ccaagggcat aagctgcgcc atcgaatgac cgacaaactc cgcacgtccg cccgcatcga
     9241 gataaagctg cggctccatc gggaacaggc acagcacgcg gttgacctga tgcacgattt
     9301 tgcccacgcg ttcccgccgc cacgcccaca ccgacgggct gacataatgc acggtcggaa
     9361 tgcccgcccg tttcagcttt tccgccacac ccaaattgaa atcgggcgca tcgataccga
     9421 caaagacatc aggtttcaac gacagcaaat cccgtaccag ccccctgcgt atccgtaaaa
     9481 tttccggcag ccgcctgacc acttcgacaa agccgcgcac cgccagccgc tcctgatcat
     9541 aaaggctctc gaaaccttcc gccttcatca gttccccgcc gatgccggta aaccgcgcct
     9601 gcgggcaacg cttgcggatg gcgcgtatca ggtgcgcccc caacaggtcg cccgacgctt
     9661 cgccgacact gacggcaatc aaagggcttt ttttatcaac catattcgtc tgtccccaca
     9721 tactttcacg tcccgatgcc gtctgaaggc ttcagacggc atcggggttc acattttcgt
     9781 cccgttttcc aacatcaccg caaactgcgc caagtccagc ggcagctccg ccttcaacac
     9841 cagcggctcg cccgtgagcg gatggttcag gtgcagctcg gacgcgtgca aaaacatccg
     9901 cttcaaaccc aacttctgca aacgacggtt cgcctgataa tcgccgtagc gttcgtcgcc
     9961 cgcaatcgga cagccttgag attgcaggtg gacgcggatt tggtgcgtgc gccccgtttt
    10021 caacgtcgcc cgcaccaaag tcaggtgcga cagcccgaca ccgtgcaaaa tgccgtctga
    10081 aaaacggctt aacacacgga acaccgtatg cgccgactgc ccgtccgcac tgacgcgcac
    10141 catcttttcg ccttgtgcgc cggtatattt gaacaggggc agtttgacat ggaaattgtc
    10201 gtccggcagt ttgcccaccc ccagcgcaag gtagattttt ttggggtggt cgttacggat
    10261 ggcttcgtga agtttgacga gcgcgctgcg tttcttcgcc accatcaaca agccgctcgt
    10321 atccttgtcc aaacgatgaa ccaactccaa atacttcgcc tccggacggg cgcggcgcaa
    10381 ctgttcgata acgccgaaac tcacgccgct gccgccgtgg acggcaacgc cggacggttt
    10441 gtcgatgacc aaaagcgcat cgtcttcgta aacaacgtca aacgcacgcg ccggtacggc
    10501 ggcacgcctt tcagacggca tttccttctc cgccacgcgc acaggcggaa tccgcaccgt
    10561 atccccctcc gcaatacggc tgtcgggttt gcagcgtttc ttgttcaacc gcacctcgcc
    10621 ggcgcggata atgcggtgga tatggctctt gggaacaccc ttgaggattt ttatcagata
    10681 gttatcaagg cgttgacccg cctcatgttc ggcaacccct atcaagctga ccgaatcttt
    10741 gcttatttcg tgcgttttca tctataatcc gaacatccgt ttcagcaaaa agcgcgcccg
    10801 ccgcgcctcc tgaaacggtt tactttaaaa cggaatttta tattaaaaac gcactccgca
    10861 aagcattttc cctgcgcctg ttcccagccg gcaggcgcag aacgtagtca agtttgaatt
    10921 gattttgccg tttcggcgcg gaagtaagac ggcagccggg cccaagcccc aaacgcaaac
    10981 cgcccgaaaa cacaggcatc acgataacaa gaagaagccg gcacccattt ttccgaccat
    11041 accgctcgga acgcggcaga cagaccgccg catcgatctt tatagccttc tgtatccgac
    11101 ccttccgcac acggttctcc ggaacgtgca tccctgcgga ttttcaactc aaaaactgat
    11161 taccggtttt atccgaaaac atcggaaaac ccagccttac ggaatgccga accgggcatg
    11221 tgccgtctga acgccgcctg cccacgaggt gatcatgaaa agaatgttat ttaacgcaac
    11281 gcaggccgaa gagctgcgcg ttgccatcgt cgacggacaa aacctgctgg atttggacat
    11341 cgaaacgctg ggcaaagaac agcgcaaagg caatatctac aaaggcatca ttacccgcat
    11401 cgagccgtcg ctggaagcgt gtttcgtgga ttacggaacc gaccgccacg gcttcttgcc
    11461 gtttaaagaa gtctcccgct catacttcca agactacaaa ggcggacgcg cgcgcatcca
    11521 agacgtgctc aaagaaggca tggaagtcat cgtccaagtc gaaaaagacg agcgcggcaa
    11581 caaaggcgcc gcgctgacca ccttcatcag cctcgccgga cgctatctgg tattgatgcc
    11641 gaacaacccg cgcggcggcg gcgtatcccg ccgtatcgaa ggtgaagagc gtcaagagtt
    11701 gaaagccgcc atggcggaac tcgacattcc gaacggcatg agcatcatcg cccgtaccgc
    11761 cggcatcggc cgcagcgcgg aagagttgga atgggacttg aactacctca aacaactctg
    11821 gcaggcgatt gaagaagcag gaaaagcgca tcacgacccc tacctgctct ttatggaaag
    11881 ctcgctgctg atccgagcca tccgcgacta tttccgcccc gacatcggcg agattttggt
    11941 ggacaatcaa gaagtttacg accaagttgc cgagttcatg agctacgtca tgccgggcaa
    12001 tataggccgt ctgaaactct acgaagacca cacgccgctg ttttcccgct tccaaatcga
    12061 acatcaaatc gaaagcgcgt tttcgcgcag cgtcagcctg ccttccggcg gcgcgatcgt
    12121 gatcgaccac accgaagccc tcgtttccat cgacgtgaac tccgcacgcg ccacacgcgg
    12181 cgcggacatc gaagacaccg cgttcaaaac caatatggaa gccgccgaag aagtcgcccg
    12241 acaaatgcgc ctgcgcgact tgggcggttt ggtcgtcatc gacttcatcg atatggaaaa
    12301 ccccaaacac cagcgcgatg tggaaaacgt cctgcgcgac gcgctcaaaa aagaccgcgc
    12361 ccgcgtgcag atgggcaaac tgtcccgttt cggactttta gaattgagcc gccaacgttt
    12421 gaaaccggct ttgggcgaaa gcagccatgt tgcgtgtccg cgctgtgccg gcaccggcgt
    12481 gattcggggc atcgaatcca ccgccctgca cgttttgcgc atcattcaag aagaagcaat
    12541 gaaagacaac accggcgaag tgcgcgcaca agtgcccgtc gatgtcgcca ccttcctgct
    12601 gaacgaaaaa cgcgccgagc tgtttgcgat ggaagagcgt ttggatgtca acgtcgtcct
    12661 gattccgaac atccacctcg aaaatccgca ctacgaaatc aaccgcatcc gcaccgacga
    12721 cgtagaagaa gacggcgaac cgagctacaa gcgcgttgcc gagccagaag aagacgaatc
    12781 cgccaaaccg ttcggcggcg aaaaagccaa agccgcccgt cccgaacccg ccgtcaaagg
    12841 cgtgcgccac accagccccg ccccgactgc cgcccccgag aaaaaaacct cctggtggga
    12901 cagcttcaaa gcttggctca aacgcatttt cggcggcagc gaaacccaag ccgcacccgc
    12961 tgccgaaacc tccgaaaaac gcagcacggc aaaccgcagc ggcagccgcg ccaacaaccg
    13021 ccgccaaaac ccgcgccgca gcaaacgcga aggcagcaaa gtagaagtcc gcgaagtggc
    13081 aggcaaaact gccagacagg aagcgcgtgc cgacacagcc gaaacgcgca acaacggcaa
    13141 ccgccgccgc aacgaacgcg gcgaccgtgc cgccgaacgt gccaacgaag cggaaatcca
    13201 aagccgcaac gtacagcctg ccgcaaccgt tgcagatgcc gcaccgtccg aaaccgaagt
    13261 gcaaaccgga aaacgccgcc gcaacggcag ccgcaacgaa cgcggccaaa ccgcgccgga
    13321 aaccgccgcc gttgccgaaa caaccgttca gacagcggaa aacacgccgt ccgaaccgca
    13381 taccgcagaa gacaaaggca gcaagcccaa atccgaacgc aaccgccgcg aacgcgacag
    13441 ccgagatgcc aaagaacgcc gcgagcgcaa caatcagcgc gaccgccgtc aaaacggcaa
    13501 aaaacgcaat attccgtctg ccgccaaaat cgagcagtac ctgaatattc acgacaccgc
    13561 cgacaaagtc cgttccgccg ccgcgcacgt tttcggcgaa accgacgcaa acgcgccgat
    13621 taccgtcagc attgccgatc cggttgcaga aagggatctt ccgacagcat ctcccgccgt
    13681 ttcaaacggc gacgcaccgg tttatgatgc ggcggaaaaa atccgccgtg ccaccgccgc
    13741 catcctgccc gaaggcgcga caccgaaagc cgaagcacag gaaatgccgt ctgaaaacgc
    13801 aacctttacg gctgcggcgg aacaggcacg ggaaaccgca caaaccggcg gactcgtcct
    13861 gatcgaaacc gaccctgccg cattgaaggc atgggcggca caacccgaag tccaagccgg
    13921 acgcggtttg cgccgttccg aacagcccaa gccgtctgaa gccgcaaccg tccctgccga
    13981 agaaatgatc caagtcgaaa cccggcaagg ctgaaccgac ggcggcaaaa agaggttctg
    14041 ttccgcagaa cctctttttt acatgggttc ggatacctgc aatgccgtct gaaacttcgc
    14101 cattcccgtg attaccgaaa cattccgcca ttcccatgat tcccgcaaca ttccgtcatt
    14161 cccatgattc ccgcaacatt ccgtcattcc cgtgaaaacg ggaatctaga acctc
//
LOCUS       DAWFHY010000053        13810 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.053, whole genome shotgun sequence.
ACCESSION   DAWFHY010000053 DAWFHY010000000
VERSION     DAWFHY010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.053"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            269..1501
                     /locus_tag="WGQ68_001373"
     CDS             269..1501
                     /locus_tag="WGQ68_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEZ0715157.1"
                     /translation="MPSAHYPEMSEKLMAVLMAMLVTLMPFSIDAYLPAIPEMAQSLN
                     ADVHRIEQSLSLFMFGTAFGQVVGGSVSDIKGRKPVALTGLIVYCLAVAAIVFVSSAE
                     QLLNLRVVQAFGAGMTVVIVGAMVRDYYSGRKAAQMFALIGIILMVVPLVAPMVGALL
                     QGLGGWQAIFVFLAAYSLVLLGLVQYFLPKPAVGGKIGRDVFGLVAGRFKRVLKTRAA
                     MGYLFFQAFSFGSMFAFLTESSFVYQQLYRVTPHQYAWAFALNIITMMFFNRVTAWRL
                     KTGVHPQSILLWGIVVQFAANLSQLAAVLFFGLPPFWLLVACVMFSVGTQGLVGANTQ
                     ACFMSYFKEEGGSANAVLGVFQSLIGAGVGMAATFLHDGSATVMAATMTASTSCGIAL
                     LWLCSHRAWKENGQSEYL"
     gene            1540..2667
                     /gene="pheA"
                     /locus_tag="WGQ68_001374"
     CDS             1540..2667
                     /gene="pheA"
                     /locus_tag="WGQ68_001374"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEZ0715158.1"
                     /translation="MLECTINYCSGEIMSQTIDELLLPHRNAIDTIDAEILRLLNERA
                     QHAHAIGELKGTGAVYRPEREVAVLRRIQDLNKGPLPDESVARLFREVMSECLAVERP
                     LTIAYLGPQGTFTQQAAIKHFGHAAHTMACPTIDDCFKQVETRQADYLVAPVENSTEG
                     SVGRTLDLLAVTALQACGEIVLRIHHNLLRKNNGSTEGIAKVFSHAQALAQCNDWLGR
                     HLPNAERIAVSSNAEAARLVAESDDGTVAAIAGRTAAEIYGLDMVAECIEDEPNNTTR
                     FLVMGHHETGASGSDKTSLAVSAPNRAGAVASLLQPLTESGISMTKFESRPSKSVLWE
                     YLFFIDIEGHRRDAQIQTALERLGERASFVKVIGSYPTAVL"
     gene            2704..3495
                     /gene="recO"
                     /locus_tag="WGQ68_001375"
     CDS             2704..3495
                     /gene="recO"
                     /locus_tag="WGQ68_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEZ0715159.1"
                     /translation="MSEYRVNHEPVFMLASSPWRESSLWVEAFSRRYGRVALLARSAR
                     KRQSELRGVLVPFVPVSVSWYGSQELKTLHRAEWVGGWRQPQGRALFGGLYVNELVLK
                     LTAREDPVPELYDALAEVMEAVCCKAAYIDDLRRFEWRLLNLLGVAPDLNRDGDGGTI
                     AAGGTYLVRPETAVFPVGKGFAVPPHAAGVVAPGQSLIDLREGSFRTAESLQQALKIT
                     RLFIRHLLPEGLKSRQVLEQIRQFDRKETARETVPTSDGTASNAV"
     gene            3519..4247
                     /gene="pdxJ"
                     /locus_tag="WGQ68_001376"
     CDS             3519..4247
                     /gene="pdxJ"
                     /locus_tag="WGQ68_001376"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEZ0715160.1"
                     /translation="MLLGVNIDHIATVRNARGTTYPSPVEAALVAETHGADLITMHLR
                     EDRRHIKDADVFAVKNAIRTRLNLEMALTEEMLENALKVMPEDVCIVPEKRQEITTEG
                     GLDVLAQQDKIAEFTKILTDAGIRVSLFIDADDRQIQAARDVGAPVVELHTGAYADAR
                     SHAEQIRQFERLQNGAHFASDLGLVVNAGHGLTIHNVTPIAQILAIRELNIGHSLIAQ
                     ALFLGLPEAVRQMKEAMFRARLLP"
     gene            4293..4400
                     /locus_tag="WGQ68_001377"
                     /pseudo
     CDS             4293..4400
                     /locus_tag="WGQ68_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4792..5331
                     /locus_tag="WGQ68_001378"
                     /pseudo
     CDS             4792..5331
                     /locus_tag="WGQ68_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
     gene            5341..6108
                     /locus_tag="WGQ68_001379"
     CDS             5341..6108
                     /locus_tag="WGQ68_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013107841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEZ0715161.1"
                     /translation="MQEIMQSIVFVAAAILHGITGMGFPMLGTTALAFIMPLSKVVAL
                     VALPSLLMSLLVLCSNNKKGFWQEIVYYLKTYKLLAIGSVVGSILGVKLLLILPVSWL
                     LLLMAIITLYYSVNGILNVCAKAKNIQVVANNKNMVLFGFLAGIIGGSTNAMSPILLI
                     FLLSETENKNRIVKSSNLCYLLAKIVQIYMLRDQYWLLNKSEYGLIFLLSVLSVIGLY
                     VGIRLRTKISPNFFKMLIFIVLLVLALKIGHSGLIKL"
     gene            6308..6685
                     /locus_tag="WGQ68_001380"
     CDS             6308..6685
                     /locus_tag="WGQ68_001380"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEZ0715162.1"
                     /translation="MIYGIGTDIVSLKRIIRLNKKFGQAFAGRILTPEELLEFPQAGK
                     PVNYLAKRFAAKEAFAKAVGTGIRGAVSFRNIGIGHDALGKPEFFYGPALSKWLEEQG
                     ISRVSLSMSDEEDTVLAFVVAEK"
     gene            6767..7576
                     /locus_tag="WGQ68_001381"
     CDS             6767..7576
                     /locus_tag="WGQ68_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0715163.1"
                     /translation="MIQDTRPLIRVVAGILLDSDGNYLLSSRPEGKPYAGYWEFAGGK
                     VEAGETDFQALQREFEEELGIRILAATPWLTKIHSYEHARVCLKFLWVNPGQWTGEPQ
                     SREGQAWSWQKAGDFTVAPMLPANGALLRSLSVPRRLYGSLKTGLHGENSMGAYRVLP
                     LGSAEESGANVLMEAAQWQDRPEHADSVWMMVQTREQWRQAQEKGADVVVWRVCDDVQ
                     AQEAAEALRQGVSVPLVLAANGQTVARYGKLWLGLGAHVVVRDETIGKNHE"
     gene            7569..7991
                     /locus_tag="WGQ68_001382"
     CDS             7569..7991
                     /locus_tag="WGQ68_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715164.1"
                     /translation="MNKNRKLLLAALLLIAFAAVKLVLLQWWQAQQPQAVAAQCDLTE
                     GCTLPDGSRVRAAAVSTKKPFDIYIEHAPVGTEQVSISFSMKNMDMGFNRYMFERQPS
                     GTWQAVRIRLPICVEGRRDFTADITIGSRTFQTAFTAE"
     gene            8040..9182
                     /locus_tag="WGQ68_001383"
     CDS             8040..9182
                     /locus_tag="WGQ68_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEZ0715165.1"
                     /translation="MNTLYTLFATCPRGLETVLSQELGSLGCTDVQAFDGGVSCRGGL
                     EQAYAANLHSRTASRILLHLTKGTYRTERDIYKLAKNINWFNWFTLQQTFKVKVEAKR
                     ANVKSIQFVGLTVKDAVCDAFRDIYDARPSVDKAAPDVRIHAFLNERNVEIFIDTSGE
                     ALFKRGYRLDTGEAPLRENLAAGLLLLAGYDGTQPFQDPFCGSGTIAVEAAWIATGRA
                     PGMMRRFGFEKLQNFDKTLWTDLRRRAEAQIRPVRAPIAGSDSDRRIVQTALDNARSA
                     EVDDIVSFSVADAQSVRPNGENGIMVSNPPYGVRLEEVRALQALYPQLGTWLKKHYAG
                     WRVGMFTSDREMPKFMRLSPKRKIPLYNGNLDCRLFLIDMVQGSNR"
     gene            complement(9483..10301)
                     /locus_tag="WGQ68_001384"
     CDS             complement(9483..10301)
                     /locus_tag="WGQ68_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0715166.1"
                     /translation="MKGMDKLRYQQDFLNIRPIFTAGEQEYLTGLSDRLPLSVLTGSV
                     RNIEEIGIDFVYSSAKLEGNTYNQYDTQALLKLGQTAGGKLYSDAVMLINLRESYRHL
                     LSGLDSPEPFDWLDFLKTTHSLISENLLEKGSGGVVRRDSVTISGTDYTPLSNPQSLD
                     TELKWLLQEAPKIENPFDRAVYLHNNLAYLQYFKDCNKRTARNCMTLSLMRSGFFPCV
                     FAPDSYPAYAEAVVAYYETGDYGLFKKYFISAYENTVNKYGPQPDMDISRNFSL"
     gene            complement(10368..11618)
                     /locus_tag="WGQ68_001385"
     CDS             complement(10368..11618)
                     /locus_tag="WGQ68_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0715167.1"
                     /translation="MTKLTRRQIIRRTAGTLFALSPIASAVAKTVRAPQFTAARIWPS
                     HTYTRLTLESTAALKYQHFTLDNPGRLVVDIQNANINTVLHGLSQKVMADDPFIRSIR
                     AGQNTPTTVRLVIDLKQPSYAQVFSLPPVGGFKNRLVVDLYPQGMDPDDPMMALLNGS
                     LNKTRRGSPEADLAQNTPAQTERGKNGRRPVIMIDPGHGGEDPGAVSPGGLQEKHVVL
                     SIARETKKQLEALGYNVFMTRNEDVFIPLGVRVAKGRARRADVFVSIHADAFTSPSAR
                     GTGVYMLNTKGATSSAAKFLEQTQNNADAVGGVPTSGNRNVDTALLDMTQTATLRDSR
                     KLGKLVLEELGRLNHLHKGRVDEANFAVLRAPDMPSILVETAFLSNPAEEKLLGSESF
                     RRQCAQSIASGVQRYINTSVLKRG"
     gene            complement(11615..12076)
                     /gene="tsaE"
                     /locus_tag="WGQ68_001386"
     CDS             complement(11615..12076)
                     /gene="tsaE"
                     /locus_tag="WGQ68_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEZ0715168.1"
                     /translation="MPDFPPVSRFLADEAATLDLGAAWSSRLNAPLVIYLEGDLGAGK
                     TTLTRGILRGLGHQGAVKSPTYAIVESYPLEPFTLHHFDLYRFSFPEEWEDAGLDELF
                     AANSVCLIEWPQQGGEFTPPADITATLTHDGGGRKCLLTAHTERGRESLPL"
     gene            12266..13078
                     /locus_tag="WGQ68_001387"
     CDS             12266..13078
                     /locus_tag="WGQ68_001387"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEZ0715169.1"
                     /translation="MENIGRQRPIGVFDSGIGGLTNVRALMERLPMENIIYFGDTARV
                     PYGTKSKATIENFSMQIVDFLLEHDVKAMVIACNTIAAVAGQKIRQKTGNMPVLDVIS
                     AGAKAALATTRNNKIGIIATNTTVNSNAYARAIHRDNPDTLVRTQAAPLLVPLVEEGW
                     LEHEVTRLTVCEYLKPLLADGIDTLVLGCTHFPLLKPLIGREAGNVALVDSAITTAEE
                     TARVLAQEGLLNTDNNNPDYRFYVSDIPLKFRTIGERFLGRTMEQIEMVSLG"
BASE COUNT         3256 a         3499 c         3790 g         3265 t
ORIGIN      
        1 atcaggacaa gacgacgaag ccgcagacag tacagatagt acggaaccga ttcacttggt
       61 gcttcagcac cttagagaat cgttctcttt gagctaaggc aaggcaacgc tgtactggtt
      121 tttgttaatc cactatatcg ttccggttcg tccggttttg ccggggcttt tgttgccgcc
      181 tgtttgtgcc ggtgtgttaa aattttccgt ttccgcgtat tgtgttttcc gccgccgggc
      241 ggtttgtttg cgaatcggac gagaatttat gccttctgcc cattatcctg aaatgagcga
      301 aaaactgatg gcggttttga tggcgatgct ggttacgctg atgccgtttt ccatcgatgc
      361 ctacctgccc gcgattcccg aaatggcgca atcgctgaac gcggatgttc accgcatcga
      421 acagagtttg agtttgttta tgttcggcac ggcgttcgga caggtggtcg gcggttcggt
      481 gtccgacatc aaagggcgca aacccgtcgc cctgaccggt ttgattgtat attgccttgc
      541 cgttgccgcc atcgtatttg tttcgagtgc cgaacagctc ctcaacctgc gcgtcgtgca
      601 ggcattcggt gcgggcatga ctgtggtcat cgtcggcgca atggtgcgcg attattattc
      661 cggacgcaaa gccgcccaga tgtttgccct tatcggcatc attttgatgg ttgtgccgct
      721 ggtcgcaccc atggtcggcg cattgttgca gggcttgggt ggctggcagg cgatttttgt
      781 ttttctggcg gcgtattcgc tggtgctgct cggtttggta cagtatttcc tgcccaagcc
      841 cgccgtcggc ggcaaaatcg gacgggacgt gttcgggctg gtggcggggc ggttcaagcg
      901 cgtattgaaa acccgtgctg cgatgggtta tctgtttttt caggcattca gcttcggttc
      961 gatgttcgcc tttctgaccg aatcttcctt cgtgtaccag cagctctacc gtgttacgcc
     1021 tcatcaatac gcttgggcgt ttgcactcaa catcatcacg atgatgtttt tcaaccgcgt
     1081 taccgcgtgg cggctcaaaa ccggcgtgca tccgcaaagc atcctgctgt gggggattgt
     1141 cgtccagttt gccgccaacc tgtcccaact cgccgccgtg ctgtttttcg ggttgccccc
     1201 gttttggctg ctggtcgcgt gcgtgatgtt ttccgtcggt acgcagggct tggtcggtgc
     1261 aaacacgcag gcgtgtttta tgtcctattt caaagaagag ggcggcagcg caaacgccgt
     1321 attgggtgta ttccaatctt taatcggcgc gggggtgggt atggcggcga ccttcttgca
     1381 cgacggttcg gcaaccgtga tggcggcaac gatgaccgcg tccacctctt gcggcattgc
     1441 gcttctgtgg ctctgctcgc atcgtgcgtg gaaagaaaac gggcaaagcg aataccttta
     1501 acggaaaatg ccgtctgaaa ccgtttcaga cggcatttga tgttagaatg cacgataaat
     1561 tactgttcag gcgaaattat gtcccaaact atcgacgaac tcctccttcc ccaccgcaac
     1621 gccatcgaca ccatcgatgc cgaaatcctg cgcctgctca acgaacgtgc gcaacacgcc
     1681 cacgccatcg gcgagctgaa aggcacgggc gcagtgtacc gccccgaacg cgaagtcgcc
     1741 gtgttgcgcc gcattcagga tttgaacaaa ggcccgctgc ccgacgaatc ggtagcacgc
     1801 ctgtttcggg aagtgatgag cgagtgcctc gccgtcgaac gcccgctgac catcgcctat
     1861 ctggggccgc agggcacgtt tacccagcag gcggcaatca aacatttcgg acacgccgcg
     1921 cacaccatgg cgtgtccgac catagacgac tgcttcaagc aggttgaaac gcgtcaggcg
     1981 gattatctgg tcgcccccgt ggaaaattcg accgaaggct cggtcggtcg cacgttagac
     2041 ctgcttgccg ttaccgcgtt gcaggcgtgc ggcgaaatcg ttttgcgcat ccaccacaac
     2101 cttttgcgta aaaacaacgg cagcaccgaa ggcattgcca aagtcttttc ccacgcgcag
     2161 gcgttggcgc agtgcaacga ctggttgggc agacacctgc ccaacgccga acggattgcc
     2221 gtgtccagca atgccgaagc cgcaaggctg gttgccgaat cggacgacgg tacggttgcc
     2281 gccatcgccg gacgcacggc ggcggaaatc tacggactcg atatggttgc cgagtgcatc
     2341 gaagacgaac cgaacaacac cacgcgcttc ttggtgatgg gacatcacga aaccggtgca
     2401 agcggcagcg acaagacttc gctggccgtt tccgcgccca accgggcagg cgcggttgcc
     2461 tcgctgctgc aaccgctgac cgaatcgggt atttccatga ccaagtttga gagccgtccg
     2521 agcaaatccg ttttgtggga atacctgttc ttcatcgaca tcgaaggaca ccgccgggac
     2581 gcgcagattc agacggcatt ggaacgcttg ggcgaacgcg cttcgttcgt caaagtcatc
     2641 ggttcgtacc cgaccgccgt tttgtagcgg cggcagcgtt cagacggcat ttccccaacg
     2701 attatgtccg aataccgagt caaccatgaa cccgttttta tgctggcatc ttcgccctgg
     2761 cgcgaaagca gcctgtgggt tgaagcattc agccgccgtt acgggcgtgt ggctttgctg
     2821 gcgcgcagcg cgcgcaaaag gcagagcgag ctgcgcggcg tattggtgcc gttcgtgccc
     2881 gtcagcgtgt cgtggtacgg cagtcaggaa ctcaaaaccc tacaccgcgc cgaatgggtc
     2941 ggcggttggc ggcagcctca gggcagggcg ttgttcggcg gattgtatgt gaacgagttg
     3001 gtgttgaaac tgaccgcccg cgaagacccg gtgcccgagt tatacgacgc gttggcggaa
     3061 gtgatggagg cggtgtgctg caaagccgct tatatcgacg acttgcgccg tttcgagtgg
     3121 cggctgctga acctgttggg cgttgccccc gatttgaacc gcgacgggga cggcgggacg
     3181 attgcggcag gcggcacata ccttgtccgc ccggaaacag ccgtcttccc cgtcggaaaa
     3241 ggatttgccg taccgccgca cgccgccggc gttgtcgccc ccgggcagag cctgatcgat
     3301 ttgcgcgaag gcagtttccg cactgccgaa agcctgcaac aggcattgaa aatcacacgg
     3361 ctttttatcc gccacctgtt gcccgagggg ctgaaatcgc ggcaggtgtt ggaacagata
     3421 cggcagtttg accgcaaaga aaccgcccgg gaaaccgtcc cgacttcgga cggcacggct
     3481 tcaaatgccg tctgaaggca gaaataaaag gaaagattat gcttttaggt gtcaacatcg
     3541 accacatcgc caccgtccgc aatgcgcgcg gtacgactta tcccagcccc gtggaggcgg
     3601 cactggttgc cgaaacgcac ggtgcggatt tgattaccat gcacctgcgc gaagaccgcc
     3661 gccacatcaa agacgcggac gtgtttgccg tcaaaaacgc catccgcacg cgcctgaacc
     3721 ttgaaatggc gttgacggaa gaaatgctcg aaaacgcgct taaagtgatg ccggaagacg
     3781 tgtgcatcgt gccggaaaaa cgtcaggaaa tcacgaccga aggcggcttg gacgtattgg
     3841 cgcaacaaga caaaattgcc gaattcacca aaatcctgac cgacgcgggc atacgcgtgt
     3901 ccttgtttat cgatgccgac gacaggcaaa tccaagccgc ccgtgatgtc ggcgcgcccg
     3961 ttgtcgagct gcacacaggc gcgtatgccg acgcgcgcag ccacgccgaa caaatcaggc
     4021 agttcgagcg tctgcaaaac ggcgcgcatt ttgccagcga tttgggcttg gtcgtcaacg
     4081 ccggacacgg actgaccata cacaacgtta cccccatcgc ccaaatcctc gccatccgcg
     4141 aactgaacat cgggcattcg ctgattgccc aagccctctt cctcggactg cccgaagccg
     4201 tgcgccaaat gaaggaggcg atgttcaggg caaggctgct gccgtaaggg caggcaaacc
     4261 ctttcagaca gcatttcacg acagggatat gttatagtgg attaaattta aatcaggaca
     4321 aggcggcgaa gccgcagaca gtacaaatag tacggcaagg caagccaacg ccgtactggt
     4381 ttaaatttaa ttcactatat gaatcaaaag tatattttat ctgcaaacaa taatagtttg
     4441 atagaagaaa ttcacaatac agtacagagt attgggtatt gtattgttcg aggtcttaat
     4501 ctaaaccatc ttgatggcag ccggagaaac aagaaattat ttgactttct atctcaatta
     4561 ggaatgctga caaaccacaa aggcgatggt tttaaatcta tattttggga tattaaatat
     4621 tgaggcgatg attatgtaat atagtggatt aacaaaaatc aggacaaggc gacgaagctg
     4681 cagacagtac agatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt
     4741 tctctttgag ctaaggcgag gcaacgccgt actggttttt gttaatccac tataaataat
     4801 gatataactt tctcggaaga tgttggagaa tgtccacttc atagtgattc atcttttagt
     4861 gaaaacccgg aaagttattt ggttatgtat gtagtaaaat cagccaatga tggaggtaat
     4921 tccctatttt taagttcatc agatattgtc aatcagttat ctaaaacaga aaccggtaaa
     4981 aaacacttaa aaacattaac gggcaattta tatccattta aaacaccagc atcatttgat
     5041 aaaaaacaag gtgtgagatg gggtaatatc ttatcggtca atactcaaat gattagattt
     5101 agaagtgatt gtatctataa aggtattgaa gaaaatagaa ataaagtatc aaaggaaatg
     5161 gtacttgcac ttgattatct tataaatgtt ataaaaaatg cgagtgatat tcaagaattt
     5221 tctgcacaag atgatggttt gattattatt gacaatgtca atggcttgca tgccagaact
     5281 gattatacgg ataaaaacag gcattatatt agagcaagaa ttactgtata aaggacggtt
     5341 atgcaagaaa taatgcaatc tatcgttttt gttgctgccg caatactgca cggaattaca
     5401 ggcatgggat ttccgatgct cggtacaacc gcattggctt ttatcatgcc attgtctaag
     5461 gttgttgcct tggtggcatt accaagcctg ttaatgagct tgttggttct atgcagcaat
     5521 aacaaaaagg gtttttggca agagattgtt tattatttaa aaacctataa attgcttgct
     5581 atcggcagcg tcgttggcag cattttgggg gtgaagttgc ttttgatact tccagtgtct
     5641 tggctgcttt tactgatggc aatcattaca ttgtattatt ctgtcaatgg tattttaaat
     5701 gtatgtgcaa aagcaaaaaa tattcaagta gttgccaata ataagaatat ggttcttttt
     5761 gggtttttgg caggcatcat cggcggttca accaatgcca tgtctcccat attgttaata
     5821 tttttgctta gcgaaacaga aaataaaaat cgtatcgtaa aatcaagcaa tctatgctat
     5881 cttttggcga aaattgttca aatatatatg ctaagagacc agtattggtt attaaataag
     5941 agtgaatacg gtttaatatt tttactgtcc gtattgtctg ttattggatt gtatgttgga
     6001 attcggttaa ggactaagat tagcccaaat ttttttaaaa tgttaatttt tattgtttta
     6061 ttggtattgg ctctgaaaat cgggcattcg ggtttaatca aactttaatt cattattaaa
     6121 tgccttaact ccttattaaa taattggcac gatgttttag aatttcaaat gcaaaaggtt
     6181 acagtgaaaa ttgttaccga caaaacccca aaagtggata ttcacgccat tttaacgccc
     6241 caagaaattg acggcattca tcatcacatt catcactacc cgcaaccaag ggcgaaggag
     6301 cgcaaatatg atttacggca tcggcacaga cattgtttcc ctcaagcgca tcatccgctt
     6361 aaacaaaaaa ttcggacagg cgtttgccgg gcgcatcctc actccagaag agctgcttga
     6421 atttccgcaa gcgggcaaac ccgtcaacta cctcgccaaa cgctttgccg ccaaagaagc
     6481 cttcgccaaa gccgtcggta cgggcatacg cggcgcggtt tccttccgca acatcggcat
     6541 cgggcatgac gcattgggca agcccgaatt tttctacggc cccgccctgt ccaaatggct
     6601 ggaggaacaa ggcatcagcc gcgtcagcct cagcatgagc gacgaagaag acaccgtatt
     6661 ggcgtttgtc gttgccgaaa aataatgccg tctgaaatgc ggcaaacccg ttgacggcat
     6721 tgcccgtccc tcatttgcac tccgaccgac caaccgcgta cccgccatga ttcaagacac
     6781 ccgacccctt atccgcgtcg ttgccggcat cctgctcgat tcagacggca actacctgct
     6841 cagctcgcgc cccgaaggca aaccctatgc cggatattgg gaatttgccg gcggcaaggt
     6901 cgaagcgggc gaaaccgact tccaagcctt gcaacgcgag tttgaagaag aactcggcat
     6961 ccgcatcctc gccgccacgc cttggctgac caaaatccat tcctacgaac acgctcgcgt
     7021 ctgcctgaaa ttcctatggg taaaccccgg ccaatggacg ggcgaaccgc aatcgcgcga
     7081 aggacaggca tggtcgtggc agaaggcggg tgattttacc gttgccccca tgctgcctgc
     7141 caacggcgcg cttttgcgtt cgctgtccgt cccgcgccgt ttgtacggca gcctgaaaac
     7201 gggtttgcac ggggagaaca gtatgggcgc gtaccgcgtc ctgcctttgg gttcggcaga
     7261 ggaaagcggt gcgaacgttt tgatggaggc ggcgcaatgg caggacagac ccgaacacgc
     7321 cgacagcgtg tggatgatgg tgcagacccg cgaacaatgg cggcaggcgc aggaaaaggg
     7381 cgcggatgtg gtcgtctggc gcgtgtgcga tgatgttcag gcacaagagg cggcagaagc
     7441 cctgcggcag ggcgtatctg tgccgctcgt acttgcagca aacggacaga cggttgcacg
     7501 ttatggaaaa ctatggctcg gattgggggc gcacgtggtg gtaagggatg aaacaatagg
     7561 gaagaatcat gaataaaaac cgtaaattac tgcttgccgc actgctgctg attgcctttg
     7621 ccgccgtcaa gctcgttttg ttgcaatggt ggcaggcgca gcagccgcaa gccgtggcgg
     7681 cgcaatgcga tttgaccgag ggttgcacgc tgccggacgg aagccgcgtc cgcgccgccg
     7741 ccgtttcaac caaaaaaccg tttgatattt atatcgaaca cgcgcccgtc ggcacggaac
     7801 aggtcagcat cagcttcagt atgaaaaata tggatatggg tttcaaccgc tatatgttcg
     7861 agcggcaacc gtcggggact tggcaggcag tacgcatccg cctgcccatc tgtgtcgaag
     7921 gcaggcgcga ttttacggcg gacattacaa tcggcagccg gacatttcag acggcattta
     7981 ccgccgaata aacctttcaa tccgccattg ccggaacatc cgtccggaaa ggacacgtta
     8041 tgaatacttt atatacactt ttcgccacct gcccgcgcgg cttggaaacc gttttatctc
     8101 aagaactcgg aagcctcggc tgtaccgatg ttcaagcgtt tgacggcggc gtttcctgcc
     8161 gcggcggatt ggaacaggcc tacgccgcca acctgcactc gcgcaccgcc agccgtatcc
     8221 tgctgcacct gaccaaaggc acataccgaa ccgagcgcga catttacaaa cttgccaaaa
     8281 acatcaactg gttcaactgg tttaccttgc agcagacgtt caaagtcaaa gtcgaggcaa
     8341 agcgtgccaa cgttaagagc atccaatttg tcggactgac cgtcaaagat gccgtctgcg
     8401 acgctttccg cgacatttac gacgcacgtc cgagcgtgga caaagccgcg cccgatgtcc
     8461 gcatccacgc ctttttgaac gaacgcaatg tcgaaatctt tatcgacact tcgggcgaag
     8521 ccctgtttaa acgcggctac cgcctggata ccggcgaagc cccgctgcgc gaaaaccttg
     8581 ctgccggact gctgctcttg gcaggctacg acggcacgca gccgtttcaa gacccgtttt
     8641 gcggcagcgg cacgattgcc gtcgaggccg cttggattgc cacaggccgc gcgccgggta
     8701 tgatgcgccg tttcggtttt gagaaactgc aaaatttcga taaaacgctg tggacagatt
     8761 tgcgccgccg cgccgaagcg caaatccgcc ccgtccgcgc cccgattgca ggcagcgaca
     8821 gcgaccgccg catcgttcag acggcattgg acaacgcacg cagtgccgaa gtggacgaca
     8881 tcgtttcctt cagcgttgcc gacgcgcaat ccgtccgtcc gaacggcgaa aacggcatca
     8941 tggtgtccaa tccgccctac ggtgtgcgcc ttgaggaagt ccgcgctttg caggctcttt
     9001 atccgcagct ggggacgtgg ttgaaaaaac actatgccgg ctggcgggtc ggcatgttta
     9061 ccagcgacag ggaaatgccc aaattcatgc gcctgtcgcc caagcggaaa attccgcttt
     9121 ataacggcaa cctcgactgc cgcctgttcc tgattgatat ggtgcaggga tcgaaccgtt
     9181 gaggaaagtg tacaaaaaag ccgtctgaaa aatgttcaga cggctttttt taatatccgg
     9241 ccgatattat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacaa
     9301 atagtacgga atcgattcac ttggtgcttc agcaccttag agaatcgttc tctttgagct
     9361 aaggcgaggc aacgccgtac tggtttttgt taattcacta taaccgaagg taagggaggg
     9421 gtgtgtccac tgtgccgccg catttgccgc gtgcggaaga tacggataac aaccccttgg
     9481 aattaaaggg agaagttgcg ggaaatatcc atatcaggct ggggaccgta cttatttacg
     9541 gtattttcgt atgccgaaat aaaatatttc ttgaataaac cgtaatcgcc ggtttcgtaa
     9601 taggctacga cagcctcggc ataggcggga taactgtcgg gggcgaatac gcaaggaaaa
     9661 aaaccggagc gcatcagcga caaggtcatg cagtttctgg cagtacgttt attgcaatct
     9721 ttaaaatatt gaagatacgc caaattatta tggagataaa cggcgcggtc gaaaggattt
     9781 tctattttgg gagcttcttg aagcaaccat tttaattccg tatccaaact ttgcgggtta
     9841 gataaagggg tgtagtctgt cccgcttatg gtaacgctgt cgcggcggac tacgccgccg
     9901 gagccttttt ccagtaagtt ttcggaaatc aggctgtggg tggtttttaa gaaatccaac
     9961 cagtcaaacg gctcggggct gtccaaaccg gacagcaggt gccggtaact ttccctcagg
    10021 ttgattaaca ttacggcatc cgaatataac ttcccgcctg cggtttgccc gagtttaagc
    10081 aatgcctgcg tatcgtattg attataagtg tttccttcca atttggcaga actatataca
    10141 aaatcgatac cgatttcttc aatattacgc acggagcccg ttaaaacaga taaaggaagc
    10201 cgatcggaca atccggtcag gtattcttgt tcgccggctg taaaaatggg gcggatattt
    10261 aaaaaatcct gttggtatct caatttatcc atacctttca tcataaaaac gcggtttggg
    10321 gcggaagtag gtttatgcct gccttcatcc gactaccgcc ttttcaatca accccgcttc
    10381 aatacggatg tattgatgta gcgttggaca cccgaggcaa tggattgggc gcactgccgg
    10441 cggaaggatt cgctgcccag caacttctct tcagcagggt tggacaggaa ggcggtttcg
    10501 accaggatag acggcatatc gggtgcgcgc aaaacggcga aattggcttc gtccaccctg
    10561 cctttgtgca agtggttgag cctgcccaat tcttcaagca ccagtttgcc gagtttgcgg
    10621 ctgtcgcgca gcgtggcggt ttgggtcata tcgagcaggg cggtatcgac gttgcggttg
    10681 ccgctggtcg gtacgccgcc gaccgcgtcg gcattgtttt gcgtctgttc caagaatttg
    10741 gcggcggagc tggtcgcgcc tttggtgttc agcatataga cccccgttcc gcgcgcggag
    10801 gggctggtga aagcgtcggc gtggatggag acgaatacgt ccgcccgccg tgctcgccct
    10861 ttggcgacac gcacgcccaa tgggatgaac acgtcttcgt tgcgcgtcat aaagacgttg
    10921 taacccaagg cttccaattg ttttttggtt tcgcgggcga tggagaggac gacgtgtttt
    10981 tcctgcaaac cgcccgggct gacggcgccg gggtcttcgc cgccgtgtcc ggggtcgatc
    11041 ataatgacgg gtctgcgccc gtttttgccg cgttcggttt gtgcgggagt gttttgagcg
    11101 aggtcggctt caggggagcc gcgacgggtt ttgttcaggc tgccgttaag cagcgccatc
    11161 atcggatcgt cgggatccat cccttgcgga tagagatcga cgacgaggcg gtttttaaag
    11221 ccgccgacgg gggggaggga aaaaacttgc gcgtaggaag gctgtttcaa atcgatgacg
    11281 aggcggacgg tggtcggcgt gttctgaccc gcgcgtatgc tgcggataaa agggtcgtcc
    11341 gccataacct tctgagacag tccgtgcaat acggtattga tgttcgcgtt ttgtatatcg
    11401 acgaccagcc tgcccgggtt gtcgagcgtg aagtgctggt atttgagcgc ggcggtgctt
    11461 tccagcgtta ggcgggtgta ggtgtgcgac ggccatatcc gtgcggcggt aaactgcggg
    11521 gcgcgtaccg ttttggcaac ggcggatgcg atggggctta gggcgaacag tgtgccggcg
    11581 gtgcggcgga tgatttgtct tcgtgtcagt ttggtcatag cggcaggctt tcgcgtcctc
    11641 gttcggtatg ggcggtcagc aggcattttc tgccgccgcc gtcgtgtgtc aatgttgcgg
    11701 tgatgtcggc gggcggggta aattcgcctc cctgttgcgg ccattcgatc aggcagacgc
    11761 tgtttgcggc aaacagttcg tcaagccccg cgtcttccca ttcttcgggg aacgagaagc
    11821 ggtagaggtc gaaatggtgc agggtaaagg gttccagcgg ataagattcg acgatggcgt
    11881 aggtcgggct tttgactgcg ccctgatgac ccaatccgcg caggatgccg cgtgtcagcg
    11941 tggttttgcc cgcacccaaa tccccttcga gataaatgac cagcggtgcg tttaaacggg
    12001 aagaccacgc cgcgcccaaa tcgagtgtgg cggcttcgtc ggcaaggaat cgggaaacgg
    12061 ggggaaaatc aggcataggg gctttacaac ttttatgtgg cggatcggag gggcaggcgg
    12121 tcggcaaacc gtgcgcccgg cggcagcatt gccgtacatt ataacagcca aatccgcatc
    12181 ctgctttcag acggcaacgg ctgtcaagaa aaagcggcgc gtgtacaata cgcggattgt
    12241 atgtttagga cggattggaa aaaggatgga aaatatcggc aggcagcgac ccatcggcgt
    12301 ttttgactcg ggaatcggcg gtttgaccaa tgtgcgcgca ctgatggagc ggctgccgat
    12361 ggagaacatc atttatttcg gcgatacggc gcgcgtgcct tacgggacga aatctaaggc
    12421 gaccatcgaa aatttctcga tgcagattgt cgatttttta ttggaacacg atgtcaaggc
    12481 gatggttatc gcgtgtaata cgattgcggc ggtggcgggg cagaaaatcc gtcaaaagac
    12541 gggcaatatg ccggttttgg acgtgatttc cgccggcgcg aaagccgcgt tggcaacgac
    12601 gcgcaacaac aaaatcggca ttatcgccac caataccaca gttaacagca atgcttatgc
    12661 gcgcgccatc catagggaca accccgacac gcttgtccgc acgcaggccg cgccgctgct
    12721 cgtccctttg gtggaggaag gttggttgga acacgaagtt acccgcctga ccgtatgcga
    12781 atacctcaaa ccattgcttg cagacggcat cgatacgctg gtgttgggct gcacgcactt
    12841 tcccttgctc aaacccttaa tcggcaggga ggcgggcaat gtcgcgctgg tcgattccgc
    12901 gattacgact gccgaagaaa ccgcacgcgt ccttgcgcag gaaggattgc tcaataccga
    12961 caacaacaat cccgactacc gtttttacgt cagcgatatt cctttgaaat tcagaaccat
    13021 cggcgagcgt tttctgggca ggacgatgga gcagattgaa atggtgtctt tgggttaaaa
    13081 cgatgacgga aagctgcccg agattacaga aacctaaaat cccgtcattc ccacgaaagt
    13141 gggaatctag acctgtcggt gcggaaactt atcggataaa acggtttctt tagattttac
    13201 gttctagatt cccgctttcg cgggaatgac gggatgcagg tttctgtgca gatgtattcg
    13261 gattccctcc tgagcgggaa tgatgggatt tttaaatgtc cgaatgccgc aaatgccgtc
    13321 tgaaaggctt cagacggcat tttttacaca attttcaccg ttttccatcc ttctcgatac
    13381 actgtaatct cgaaacccgt cattcccgcg caggcgggaa tccagaccgt tcggtttcgg
    13441 tttttttgag gtttcgggta acttctaaac cgtcattccc gcgcaggcgg gaatccagac
    13501 cgttcggttt cggttttttt gaggtttcgg gtaacttcta aaccgtcatt cccgcgaaaa
    13561 cagaaaatca aaaacagaaa cctcaaatcc cgtcattccc gcgcaggcgg gaatctagac
    13621 attcaatgct aaggcaattt atcgggaatg actgaaactc aaaaaactgg attcccactt
    13681 ttgtgggaat gacgggatat aggtttccat gcggacgcgt tcggattcac gactgcgcgg
    13741 aaatgacggg attttggtgt attccctaaa aaaataaaaa aacatttgca actttgttaa
    13801 aaataaaggc
//
LOCUS       DAWFHY010000054        13689 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.054, whole genome shotgun sequence.
ACCESSION   DAWFHY010000054 DAWFHY010000000
VERSION     DAWFHY010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13689
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.054"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(316..1122)
                     /locus_tag="WGQ68_001388"
     CDS             complement(316..1122)
                     /locus_tag="WGQ68_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HEZ0715170.1"
                     /translation="MPTVFIFLTAVYAGLGAFAWHCQQQGLGRDYPWKTELPVLGAAL
                     TVHGAALLMPVIQDKIIIMGFGYSGSLIVWMMLFIYFAGSFFYSLRGVQLLLYPCAAL
                     MLLSGLVFPGKFSGYEITDLPFMLHIGTSLLAYGLFGIATLLSVLTLLLNRSLHRRNF
                     SKLAGFLPSLLSLEKLMFQAMWAGFILLTYSVVSGTFFAEAVFGKPMTFTHKTVFGIL
                     SWLIYGGLLLKHSMTAWRGKKAAVWTIIGFVSLMIAYMGSKFVLEIILKR"
     gene            1343..2713
                     /gene="ffh"
                     /locus_tag="WGQ68_001389"
     CDS             1343..2713
                     /gene="ffh"
                     /locus_tag="WGQ68_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEZ0715171.1"
                     /translation="MLDNLTGRFSNVLKNIRGQAKLTEDNIKEALREVRLALLEADVA
                     LPVVKEFVNNVKEKALGQEVVGSLTPDQAFIGVVNKALTELMGRENKTLDLSVAPPAV
                     VLMAGLQGAGKTTTAGKLARLLKNDQKKKVLVVSADVYRPAAIEQLRLLAEQVGVDFF
                     PSDTNQKPVEIATAAVDYAKKHFYDVLMVDTAGRLAIDEEMMNEIKALHAAVNPVETL
                     FVIDAMLGQDAVNTAQAFNEALPLTGVVLTKMDGDSRGGAALSVRHVTGKPIKFIGVG
                     EKINGLEPFHPDRLASRILGMGDVLTLIEDVQKGIDEEAAAKMAKKLQKGKGFDLNDF
                     KEQIQQMRNMGGLENLMSKMPGELGQISKQIPEGTAEKAMGKVEAIINSMTPKERANP
                     ALLKASRKRRIAMGAGTTVQEVNKLLKQFEQMQQMMKMFSGNGLGKLMRMAKGMKGMK
                     GMFPGL"
     gene            complement(3044..3739)
                     /locus_tag="WGQ68_001390"
     CDS             complement(3044..3739)
                     /locus_tag="WGQ68_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0715172.1"
                     /translation="MKLKTLALTSLTLLALAACSKQAETSVPADSAQSNTSAPATPAA
                     LTEGINYTVLSNPIPQQQAGKIEVLEFFGYFCPHCAHLEPVLSEHIKTFKDDTYMRRE
                     HVVWGDEMKPLARLAAAVEMAGESDKANSHIFDAMVNQKINLADTDTLKKWLSEQTAF
                     DGKKVLAAFEAPESQARAAQMEELTNKFQISGTPTVIVGGKYQVEFKDWQSGMTTIDQ
                     LVDKVREEQKKPQ"
     gene            complement(3910..4569)
                     /locus_tag="WGQ68_001391"
     CDS             complement(3910..4569)
                     /locus_tag="WGQ68_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEZ0715173.1"
                     /translation="MKTAILIDGAFFIKRVRSLGLSQNPHDAKRIADLAFRYSMLHLK
                     DKINGTDQMDDLYRIFFYDCVPFEKKMHNPVSHRAIDFSKTPEAIFRRNLHTELKKKR
                     KLALRLGKLSDNTAWILKPQVMKNLLKNPSTQITENDVVLDVKQKGVDMRIGLDISSI
                     TLKKQADKIILFSGDSDFVPAAKLARREGIDFILDPMWNHVPPDLMEHIDGLKTKCPK
                     P"
     gene            complement(4772..6883)
                     /locus_tag="WGQ68_001392"
     CDS             complement(4772..6883)
                     /locus_tag="WGQ68_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0715174.1"
                     /translation="MKISFHLALLPTLIIASFPVAAADTQDNGEHYTATLPTVSVVGQ
                     SDTSVLKGYINYDEAAVTRNGQLIKETPQTIDTLNIQKNKNYGTNDLSSILEGNAGID
                     AAYDMRGESIFLRGFQADASDIYRDGVRESGQVRRSTANIERVEILKGPSSVLYGRTN
                     GGGVINMVSKYANFKQSRNIGTVYGSWANRSLNMDINEVLNKNVAIRLTGEVGRANSF
                     RSGIDSKNVMVSPSITVKLDNGLKWTGQYTYDNVERTPDRSPTKSVYDRFGLPYRMGF
                     AHRNDFVKDKLQVWRSDLEYAFNDKWRAQWQLAHRTAAQDFDHFYAGSENGNLIKRNY
                     AWQQTDNKTLSSNFTLNGDYTIGRFENHLTVGMDYSREHRNPTLGYSRAFTASIDPYD
                     RASWPASGRLQPVLTQNRHKADSYGIFVQNIFSATPDLKFVLGGRYDKYTFNSENKLT
                     GSSRQYSGHSFSPNIGAVWNVTPAHTLYASYNKGFAPYGGRGGYLSIDTLSSAVFNAD
                     PEYTRQYETGVKSSWLDDRLSTTLSAYQIERFNIRYRPDPKNNPYIYAVSGKHRSRGV
                     ELSAIGQIIPKKLYLRGSLGVMQAKVVEDKENPDRVGIHLNNTSNVTGNLFFRYTPTE
                     NLYGEIGVTGTGKRYGYNSRNKEVTTLPGFARVDAMLGWNHKNVNITFAAANLLNQKY
                     WRSDAMPGAPRTYTARVNYSF"
     gene            complement(7069..7674)
                     /locus_tag="WGQ68_001393"
     CDS             complement(7069..7674)
                     /locus_tag="WGQ68_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEZ0715175.1"
                     /translation="MTRQSLQQAAESRRSIYSLNKNLPVGKDEVVQIVEHAVLHTPSS
                     FNSQSARVVVLFGEEHDKVWQFVEDALRAAVPADSFEPTAQKLNLFKAGAATILFYED
                     QNVVKGLQEQFPAYAANFPVWADQANAMVQYAVWTTLAAVGVGANLQHYNPLPDAAIA
                     KAWNIPENWLLRAQMVIGGIEGAAGEKTFEPVAERLKVFGA"
     gene            complement(7723..8121)
                     /locus_tag="WGQ68_001394"
     CDS             complement(7723..8121)
                     /locus_tag="WGQ68_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0715176.1"
                     /translation="MSDCCNRIQPVLLSVLRIVTAYLFLLHGTSKIFAFPIEMGSGSP
                     GGLLLLAGILEIVGGILLVLGLFARPAAFVLSGQMAVAYFMAHASGNALFPIANGGES
                     AVLFCFVFLYIAAAGGGAWSLDRLFFKRKA"
     gene            8229..9140
                     /locus_tag="WGQ68_001395"
     CDS             8229..9140
                     /locus_tag="WGQ68_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEZ0715177.1"
                     /translation="MDTLFSLKVFRQVVQSGGFTRAADALGISTAMASKHVSHLENTV
                     QAKLLHRNSRNLSLTEAGEEYYRQCSYALDTLDDAAQKAAGGTEKPQGLLRVTMPLWF
                     AGSQICNWLAEYRERYPEVTLELILDNRHVDLIAEGVDLALRVSQTLSPSLIARPLAE
                     IEFALLASPDFLRRNGVPETPEEVAGLPAVLPTYTNQQKLDLTRKSDGKKYRLELTPV
                     IRTDNTLMMREMIKAGACIGYQPLWAAEHDLRCGSLVRLLPEYTVPTDRLNAVYADRA
                     FLSAKVRSFIDFLNEKIASRKGCRNAV"
     gene            9130..10431
                     /locus_tag="WGQ68_001396"
     CDS             9130..10431
                     /locus_tag="WGQ68_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HEZ0715178.1"
                     /translation="MPSETARPLYADTCAAHTLVWFRQNLRIRDNAALCAAVAEGLPV
                     IGIWIDDAETDNPRRAAFYRQSAAELAQGLAGRGIPLYTAASPAELVRLAVRLNIRAV
                     IADESHTFADKLADNALWHELDKHGIALTFVNDRAVFGKTDLIPDGGTAHTDFDRYRE
                     VWLDRFSKQPPAGPDLFAAYRQPFPENLSAPPPAALSDGIFLPQNSGETAAWRQWRRF
                     LEQADSYSVLKDFPSRKHTSLMGAYLSAGCISPRLLARESLERRLNAWADNIIRRDFF
                     LQLALQHTDDDPSDGNPEHTLRLTLWQQGQTGIPIIDAAMRCLHKTGSLHPALRRLSA
                     DFFCHVLNLPRREGEIWFARQLTDFDAAINQGNWRLAASRHTYPDIAAASYRTDPDGT
                     FIKRHIPELAHLSADTVHTPWRFACSVDTHGYPAYPVAGIP"
     gene            complement(10499..10723)
                     /locus_tag="WGQ68_001397"
     CDS             complement(10499..10723)
                     /locus_tag="WGQ68_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715179.1"
                     /translation="MVFWLQRLKYPLLLWIADMLLYRLLGGAEIECGRCPVPPMTDWQ
                     HFLPAMGTVSAWVAVIWAYLIIESEKNGRY"
     gene            10826..12976
                     /gene="dinG"
                     /locus_tag="WGQ68_001398"
     CDS             10826..12976
                     /gene="dinG"
                     /locus_tag="WGQ68_001398"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HEZ0715180.1"
                     /translation="MLTDLEKNAIRDHYQNIGKNLPGFRPRASQREMIAAVANAFSRT
                     LAREEGGEPPKREGESIAVIEGPTGVGKSLAYLLAGGIMAQTRGKRLIVSSATVALQE
                     QLVDRDLPFLVEKSGLELSFALAKGRGRYLCPYKLYQLTQSNAQQNLLGFEAPAVLWD
                     SKPKPEELKLLRDIADEFSARRFNGDRDAWPEKIDDAIWLKVTNDRHGCLKTACPNRP
                     ECPFYLARDVLETVDVVVANHDLLLADISMGGGVILPAPENSFYCIDEAHHLPKKALS
                     RFAAEHSWNIAVWTLEKLPQLTGKIAALTDKAELANLADEAAASLLDSLHEWQFHLAE
                     EPSLSMGPSENDRRTNSEPTWLWEDGKIPEGLETTVSNTAIAARSLLKHVVGLNDALS
                     AARREKEQDSALLDRLTGEFGLFIARIEQISAVWDLLSTVPLEGEEPLAKWITRRADD
                     KNDYIFNASPISSASHLANSLWRRAAGAVLTSATLQSLGNFNLILRQTGLQWLPETTT
                     LALKSPFDFEKQGELYIPPIYASPKDPEAHTAAIIEWLPKLISPTEAIGTLVLFSSRK
                     QMQDVALRLPGDYLPLLLVQGELPKAVLLQKHHRAIEEGKASIIFGLDSFAEGLDLPG
                     TACVQVIIAKLPFAMPDNPIEKTQNRWIEQRGGNPFIEITVPEAGIKLIQAVGRLIRT
                     EQDYGRVTILDNRIKTQRYGQQLLAGLPPFKRIG"
     gene            13013..13627
                     /locus_tag="WGQ68_001399"
     CDS             13013..13627
                     /locus_tag="WGQ68_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HEZ0715181.1"
                     /translation="MNFTRLLNQVLSTVQKKGNTFSDSPLNSFGGGALVAGIASMLLN
                     GKNRKTITKIGSTAALGYLAYRGYQMWQQNKGRATVTQSDFQPAGETEETYSRTVLRT
                     MIAAAASDGMIDEAKRRTIEQESGTDPETAAWLAAEYRLPASIEDIAAAVGNDEALAA
                     EAYLAARLVCADLSRKETVFLARLSQALKLDDNLVESLERQLGF"
BASE COUNT         3336 a         3797 c         3470 g         3086 t
ORIGIN      
        1 cgaattataa agtatctctt ccgcctgtca agatatctct cgatatcccc aacattctgt
       61 gctatacttt tcagttcgtc cgccgcttct gcagcagcga agaaccgaac tatacgccca
      121 cagggaaaaa cggtcaatgc tttcagtggg atttttttgg ggaaattcgt catgtcgctg
      181 tcggataagg ttttttattt ctgctaaatg ctgcgccgcc tccaacaatc ctttcctctc
      241 cctcctccgg ctggtgcgcc tttgtgaata tgccgtctga aactcgggga ctcagacggc
      301 atctgttggc tcttcttatc ttttcagaat gatttccaat acgaacttgc tgcccatata
      361 ggcaatcata aggctgacaa atccgatgat ggtccacacg gcggcttttt taccgcgcca
      421 tgcggtcatg ctgtgcttga gcagcagtcc gccgtaaatc agccatgaca atatgccgaa
      481 tacggtttta tgggtaaagg tcatgggttt gccgaatacg gcttcggcaa aaaatgttcc
      541 gctgacgacg gaataagtca gcaggatgaa acctgcccac atggcctgga acatgagttt
      601 ttccaaactg agcagcgacg gcaggaatcc tgcgagcttg gagaagttcc tgcggtgcag
      661 gctccgattc agcagcaggg tcaaaacgga caataatgtt gcgatgccga acagcccgta
      721 tgcgagcagc gaagttccga tatgcagcat aaagggaagg tcggtaattt catatcccga
      781 gaattttcca ggaaaaacca aacctgacag cagcatcagt gcggcgcaag gatacagcag
      841 caactgcact ccgcgcagcg aataaaagaa gctgccggca aaataaataa acagcatcat
      901 ccaaacaatc aggctgccgg aatacccaaa gcccataatg atgattttgt cttgaatgac
      961 cggcataagc agtgccgcgc catggacggt caatgccgcg cccaaaaccg gcaattccgt
     1021 cttccacggg taatcccggc cgagcccctg ctgttggcag tgccatgcaa atgcacccaa
     1081 tcctgcgtaa accgccgtca aaaagatgaa aactgtcggc atggtggact ttctctaatc
     1141 tatactgttg cgctgtatgc ggccgcttat gaaatattgg aacttttaac gttggaattg
     1201 taaaatcccc gtttcgggca agccttgacg gatttgccga tatgctgtcc ggcacacaag
     1261 ccgcatcaaa ttattttgat tttattttaa caaagaatgc ccctgatggg gcaagctatt
     1321 cttattcaga ccaaggacca gtatgttaga caatttaacc ggccgcttca gcaatgttct
     1381 caaaaacatc cgcgggcagg ccaaactgac cgaagacaat attaaagaag ccttgcgcga
     1441 agtccgcctc gccctgcttg aggcggatgt cgccctgcct gtcgtcaaag agttcgtcaa
     1501 caacgtcaaa gaaaaggccc tcggtcagga agtggtgggc agcctgacgc cggatcaggc
     1561 atttatcggc gtggtcaaca aagccctgac cgagctgatg ggcagggaaa acaaaacgct
     1621 ggatttgtcg gttgcgccgc ccgccgtcgt gttgatggca ggtttgcagg gcgcgggcaa
     1681 gacgacgacc gccggcaaac tcgcccgcct gctgaaaaac gatcagaaga aaaaggtttt
     1741 ggtcgtatct gccgacgttt accgccctgc cgcgattgaa caactgcgtc tgttggccga
     1801 acaggtcggc gtggactttt tcccatccga taccaaccaa aaaccggttg agattgcaac
     1861 tgccgccgtc gattacgcca aaaaacattt ttacgatgta ttgatggtcg ataccgccgg
     1921 ccgtttggca atcgatgagg agatgatgaa cgaaatcaaa gcccttcacg cggcggttaa
     1981 cccggtggaa actttgttcg tcatcgatgc gatgctgggt caggatgcgg tgaacactgc
     2041 tcaggcattt aatgaagccc tgccgctgac cggtgtcgta ttgaccaaga tggacggcga
     2101 ttcgcgcggc ggcgcagctt tgtccgtgcg ccacgtaacc ggcaagccga ttaaatttat
     2161 cggtgtcggc gaaaaaatca acggcctcga acctttccac cccgaccgtc ttgccagccg
     2221 tatcctcggc atgggcgacg tattgaccct gattgaagac gtccaaaaag gtattgatga
     2281 agaagccgcc gccaaaatgg cgaaaaagct acaaaaaggc aaaggcttcg acctcaacga
     2341 ctttaaagaa caaatccagc aaatgcgcaa tatgggcggt ttggaaaacc tgatgtcgaa
     2401 aatgccgggc gaactgggtc aaatatcgaa acaaatcccc gaaggaacgg ctgaaaaagc
     2461 gatgggcaaa gtggaagcca tcatcaactc gatgactcca aaagaacgcg ccaaccctgc
     2521 cctgctcaaa gccagccgca aacgccgtat tgcaatgggt gcgggcacga ccgtacagga
     2581 agtgaacaaa ttgctcaaac agtttgaaca aatgcaacaa atgatgaaga tgttcagcgg
     2641 caacggcttg ggcaaactga tgcgtatggc gaaaggaatg aagggaatga aaggaatgtt
     2701 cccgggtttg taagccgatt tgaccgaaaa cgccgtctaa aatttcagac ggcgtttttg
     2761 ttttatattc tgatttatag tggattaaca aaaatcagga caaggcggcg agccgcagac
     2821 agtacagata gtacggaacc gattcacttg gtgcttcagc accttagaga atcgttctct
     2881 ttgagctaag gcgagccaac gccgtactgg tttttgttaa tccgctatat attctgattt
     2941 aaaaccataa ggctttaagc aatcatctct tctataaagc ctaaatacaa aaggccgtct
     3001 gaaatcctat tttcagatgg cctttactca ttcaatcctc aacttattgc ggctttttct
     3061 gctcttcgcg tactttatcc accaactggt caatcgtggt cataccggac tgccagtctt
     3121 taaattcaac ttggtatttg ccgccgacga tcacagtcgg tgtgccgctg atttggaatt
     3181 tattggtcaa ctcttccatt tgagccgcac gcgcttggct ttcaggagcc tcaaatgcag
     3241 ccaatacttt tttgccgtca aacgctgttt gctcggacag ccattttttc agggtatcgg
     3301 tatcggccag attgattttt tgattaacca tcgcatcgaa aatatggctg ttggctttat
     3361 ctgattcacc ggccatttcc actgcggccg ccaaacgtgc caaaggtttc atttcatcac
     3421 cccacacgac atgctcccgg cgcatatagg tatcgtcttt aaacgttttg atgtgctcgc
     3481 tcaagaccgg ctcaagatgg gcgcaatgcg ggcagaagta gccgaagaat tccaatactt
     3541 cgattttgcc ggcctgctgt tgcggaatcg gattggacaa tacggtgtaa ttgatacctt
     3601 cggtcaatgc ggcaggggtt gccggagcgg atgtgttgct ttgggcgctg tctgccggaa
     3661 cgctggtttc agcctgtttg ctacaagcgg ccaatgccaa cagggtcaat gaagtcaaag
     3721 ctaaggtttt cagtttcata ggtatctctt gtgtgtcaga ttaatgtgcg gattttatgg
     3781 cattttattg aaggcgcgtt cgatttgagt gaaaaaatgt gtttagtttt atttcagcct
     3841 ttataaaggc cgtctgaaat atggcttaga taattagcct tattttcaga cggcctttgt
     3901 tctagtaatt tacggtttcg gacattttgt cttcaatccg tcgatgtgtt ccattaagtc
     3961 aggtgggaca tgattccaca taggatcaag aataaaatcg ataccttccc gtctggctaa
     4021 tttggctgct gggacaaaat cggaatcacc agaaaacaag atgattttat cggcttgttt
     4081 ttttaaggta atagatgaaa tatccaagcc tatacgcata tctacacctt tttgtttaac
     4141 atcgagcacg acatcgtttt ccgtaatttg agttgacggg tttttcagaa gatttttcat
     4201 gacttggggt tttaatatcc atgctgtatt gtccgacaac ttgcctaaac gtaacgctaa
     4261 tttacgcttc ttcttcaatt cggtatgcag attgcgacga aatatggctt ccggagtctt
     4321 tgaaaaatct atggcacgat gagatacggg attgtgcatt ttcttttcaa acggcacgca
     4381 gtcgtaaaag aaaattctat ataaatcatc catttgatct gtgccgttga ttttatcttt
     4441 cagatgcagc atcgaatatc ggaaagccaa atcagcaatt ctttttgcat cgtgtggatt
     4501 ttgagacagg cctaatgacc gtacccgctt aataaaaaat gcaccgtcaa tcaaaatggc
     4561 ggttttcata ttgcttcccc tatatttgtc aaagatataa aaaagccctt gggatcggca
     4621 tacccgctat gatttaatgg gtaatgcgta ctgccaaggg cgctatttct ttttggatta
     4681 tactgatgta gataaagcct gtcaaaaagt tcgtgtgctg aatttacgga ttatcaggat
     4741 tttcagacgg cattctgttg cacgaacggg attagaaact gtaattaacc cgcgccgtat
     4801 aagtgcgcgg cgcgccgggc atggcatccg aacgccaata tttttgattg agcagattgg
     4861 ctgcggcaaa ggtaatgtta acatttttat ggttccagcc aagcatggca tcaactcggg
     4921 caaagcctgg aagcgtagtc acttctttat ttcttgagtt gtaaccgtag cgtttgcctg
     4981 taccggttac gccgatttcg ccgtagaggt tttcggtcgg ggtataacgg aaaaacaggt
     5041 tgccggtaac gttgctggtg ttattcaaat ggatgcccac tcggtcggga ttttctttgt
     5101 cttcaacgac tttcgcctgc atcacgccca acgaaccgcg cagatagagt tttttaggga
     5161 tgatttgccc gatggcggac aattccacgc cgcgcgaacg gtgtttgccg ctaaccgcat
     5221 aaatataagg gttgtttttt ggatcggggc ggtagcggat attgaagcgt tcgatttggt
     5281 aggcggacaa tgtggtgctg aggcggtcgt ccagccaact gcttttaacg ccggtttcgt
     5341 attggcgggt gtactcgggg tcggcgttga acacggcgga agacaacgta tcgatactca
     5401 aatagccgcc gcgtccgcca taaggcgcga agcctttgtt atacgaggcg tagagcgtgt
     5461 gggcgggcgt tacgttccac actgcgccga tgttggggct gaacgagtgt ccgctgtatt
     5521 ggcggctgct gccggtgagt ttgttttcgg aattaaaggt gtatttgtcg taacggccgc
     5581 cgaggacgaa tttcaaatcg ggcgtggcgg agaagatgtt ttgcacgaag atgccgtagg
     5641 aatcggcctt gtgtcggttt tgagtgagga cgggctgcaa tctgcccgaa gccggccagc
     5701 ttgctcggtc gtatggatcg atggaagcag taaaggcgcg gctgtaacct aaggtcgggt
     5761 tgcggtgttc gcggctgtaa tccatgccta cggtcaggtg gttttcaaaa cggccgatgg
     5821 tgtagtcgcc gtttagcgtg aaattggacg acagggtttt gttgtcggtc tgctgccagg
     5881 cgtagttacg tttgattaag ttgccatttt cgctgcctgc atagaaatga tcaaaatcct
     5941 gcgccgccgt gcggtgggcg agctgccatt gggcacgcca tttgtcgttg aaggcgtatt
     6001 caaggtcgga acgccaaact tgcagcttgt ctttgacaaa atcgttccgg tgggcgaacc
     6061 ccatgcggta aggcagtccg aagcggtcgt acacggactt ggtcggactg cggtcgggcg
     6121 tgcgctccac attgtcgtag gtgtattgcc ccgtccactt caagccgttg tcgagtttga
     6181 cggtaatgct gggcgaaacc atgacatttt tgctgtctat gccgctgcgg aacgaattgg
     6241 cgcgcccgac ttcgccggtg agacggatgg cgacgttttt gttcagcact tcgttgatgt
     6301 ccatattcag gctgcggttt gcccacgaac cataaaccgt accgatatta cggctttgtt
     6361 tgaagttggc gtatttgctg accatgttga tgacaccgcc gccgttggta cgcccataaa
     6421 gcacggagga cggacctttc aggatttcca cgcgctcgat gttggcggtg ctacggcgca
     6481 cctgcccgct ttcgcgtacg ccgtcgcggt aaatatcaga tgcgtcggct tgaaagccgc
     6541 gcaggaaaat gctttcgccg cgcatatcgt aggcggcgtc gatgccggca ttgccttcga
     6601 ggatggaact caaatcgttc gtgccgtaat ttttgttttt ctggatattg agcgtatcga
     6661 tggtttgcgg cgtttctttg atgagctgtc cgttgcgggt aacggcggct tcgtcgtagt
     6721 tgatgtagcc tttgagtacg ctggtgtcgg actgtccgac cacggaaacg gtaggtagcg
     6781 tggcggtgta atgttcacca ttgtcctgcg tatcggcggc agcaacaggg aaggaagcaa
     6841 taatcagtgt gggtaataaa gctaaatgaa atgatatttt catttttata ctcaatttaa
     6901 caaaacaacc gaattatatt gcctcacgga ggaaatgaga ataatttctt ttaactatat
     6961 tgaacatgat atttgtaaac aaaggtctca gaatgcggaa aactcgccgc ctgatactga
     7021 aaaatgccgt ctgaacaggg ttcagacggc atttttttga ccgcgaaatt atgcgccgaa
     7081 cactttcaaa cgttctgcaa ctggttcaaa ggtcttttca cctgccgccc cttcaatacc
     7141 gccgataacc atttgcgcgc gcaacaacca gttttcgggg atattccacg ctttggcaat
     7201 cgccgcatcg ggcaagggat tgtaatgttg caggtttgca cctacgccga ccgcggcaag
     7261 tgtcgtccaa acggcatact gcaccatcgc gttcgcctgg tccgcccaaa cgggaaagtt
     7321 ggcggcataa gcagggaact gctcctgcaa acctttgacg acattttgat cttcataaaa
     7381 caaaatggtt gccgcacccg ccttaaacag gttcaatttt tgcgcggtcg gttcaaaact
     7441 gtcggcaggc acggcggcac gcagcgcgtc ttcgacaaat tgccacacct tatcatgctc
     7501 ttcgccaaac agcacgacca cgcgggcaga ttgggaattg aacgaagaag gtgtgtgcaa
     7561 aacggcgtgt tcgacgattt ggacaacttc atctttgccg acgggcagat ttttatttaa
     7621 cgaataaatg gaacggcggc tttcggcagc ctgttgcaga gattgacggg tcatttcttt
     7681 tcctcaaaaa cattcaacaa aatacacttt aggcagtccg attcaggctt tacgcttgaa
     7741 aaacagcctg tccagcgacc atgctccgcc gcccgccgcc gcgatataga ggaatacgaa
     7801 gcagaacagc actgcggact cgccgccgtt ggcaatcggg aacaaagcat ttccggaagc
     7861 gtgcgccata aaataggcaa ccgccatctg gccggacaaa acaaacgcgg cagggcgcgc
     7921 aaacaggccc aacaccagca aaatgccgcc gacaatttct aaaataccgg caagcagcaa
     7981 cagcccgccg ggcgaaccgc tgcccatttc aatggggaag gcgaagattt tcgacgtacc
     8041 gtgcaacaaa aacaggtaag cggttacgat acgcaaaaca gaaagcaaaa ccggttggat
     8101 acggttgcag caatcggaca taaaagctcc tttgatattt gtcttcattt caggcggtag
     8161 agtatagatg tttccccttt tcaaatatat actgctttta ttgatacaac ttttccacac
     8221 aagaaaatat ggacaccctg ttcagcctca aggttttccg ccaagtcgtc caaagcggcg
     8281 gcttcacccg cgccgccgac gcgctcggca tctccaccgc aatggcaagc aaacacgtca
     8341 gccacttgga aaacaccgtc caagccaaac tcctgcaccg caacagccgc aacctcagcc
     8401 tgaccgaagc cggggaagaa tactaccggc aatgcagtta cgcgctcgac acgctcgacg
     8461 atgccgcgca aaaagccgcc ggggggacgg aaaaaccgca ggggctgctg cgcgtaacga
     8521 tgccactgtg gtttgccggc agccagatat gcaactggct ggcggaatac cgcgaacgtt
     8581 atcccgaagt gacattagaa ctgattttgg acaaccgcca cgtcgatttg attgccgaag
     8641 gcgtggattt ggcgttgcgc gtttcccaaa ccctgtcccc gtcgctgatt gcgcgcccgc
     8701 tggcggaaat cgaatttgcc ctgctcgcct cgcccgattt cctgaggcgc aacggcgtac
     8761 cggaaacgcc ggaagaagtg gcggggctgc ccgccgtcct gccgacctac accaaccagc
     8821 agaaactcga cctcacccgc aaatcggacg gcaaaaaata ccggcttgaa ctgacccccg
     8881 tcatccgtac cgacaacacg ctgatgatgc gcgaaatgat taaggcgggc gcgtgcatcg
     8941 gttatcagcc gctttgggcg gcggaacacg atttgcgctg cggctcgctg gtgaggctgc
     9001 tgcccgaata taccgtcccg accgaccgcc tgaatgccgt ttatgcggac agggcgttct
     9061 taagcgcgaa agtccgcagc ttcatcgatt ttctgaacga aaaaatcgcc agcaggaaag
     9121 gctgccgaaa tgccgtctga aaccgcccgc cccctttatg cggacacgtg cgccgcacac
     9181 acgctcgtct ggttccgcca aaacctccgc atccgcgaca acgccgcctt atgcgccgcc
     9241 gttgccgaag gtttgcccgt tatcggtatt tggattgacg atgccgaaac agacaaccct
     9301 cgccgcgccg cgttctaccg ccaatccgcc gccgaactcg cccaagggct tgcagggcgc
     9361 ggcatcccgc tctacacggc ggcatcgcct gccgagctcg tccggctcgc cgtccgcctc
     9421 aatatccgcg ccgtcatcgc cgacgaatcc catacttttg ccgacaaact cgccgacaac
     9481 gccctttggc atgaattgga caaacacggc atcgcgttaa ccttcgtcaa cgaccgtgcc
     9541 gttttcggca aaaccgacct gatacccgac ggcggcacgg cacacaccga tttcgaccgc
     9601 taccgcgaag tatggctcga ccgcttttcc aagcagcccc ccgccggtcc ggacctattc
     9661 gcggcatacc gccaaccctt ccccgaaaac ctttccgccc cgccgcctgc cgccctttca
     9721 gacggcatct tcctgccgca aaacagcggc gaaacggcgg cttggcggca gtggcggcgg
     9781 tttctcgaac aggcggattc ctactccgtt ttaaaggatt tcccctcgcg caaacacacc
     9841 tcgctgatgg gcgcgtattt gagtgccggc tgcatctcgc cgcgcctgct cgcgcgggaa
     9901 agcctcgaac gccgtctgaa cgcgtgggcg gacaacatca tccgccgcga ttttttcctt
     9961 caacttgcct tgcagcacac ggatgacgac ccttcagacg gcaatcctga acacaccctg
    10021 cgcctgacgc tttggcagca gggccagacc ggcattccga ttatcgatgc cgcgatgcgc
    10081 tgtttgcaca aaaccggcag cctccacccc gccctgagac gcttgagcgc ggattttttc
    10141 tgccacgttt taaacctccc ccgccgcgaa ggcgagatat ggtttgcccg acagctgacc
    10201 gatttcgatg cagcaatcaa ccaaggcaac tggcggcttg ccgcctcacg gcacacctac
    10261 cccgacattg ccgccgcctc ataccgtacc gacccggacg gtacttttat caaacggcac
    10321 attcccgaac tcgcccacct gtccgccgac accgtccaca cgccttggcg gtttgcctgt
    10381 tcggtcgata cccacggcta tcccgcctat cctgtcgccg gtattcccta agcggtacgg
    10441 caacctaaaa caaatgccgt ctgaacttcc gtttcagacg gcattgcgtc cggatgactc
    10501 aatatcttcc gtttttttca ctttcaataa tcaggtatgc ccaaatcacc gccacccaag
    10561 ccgacaccgt tcccatcgcc ggcaaaaaat gctgccaatc cgtcatcggc ggcacagggc
    10621 aacggccgca ttcgatttcc gcgccgccca acaaccggta cagcaacata tccgcaatcc
    10681 aaagcaacaa cgggtatttc aaacgctgca accaaaaaac caaccacgtc cgcacgattc
    10741 acctcacccc ccattccgac aacgatattg taccgcttcc gtgcaatgtt aaaataagcc
    10801 cacttctacc gccgtacaaa acgccatgct caccgattta gaaaaaaacg ccatccgcga
    10861 ccattaccaa aacatcggca aaaacctgcc cggtttccgt ccgcgcgctt cgcagcggga
    10921 aatgattgcg gcggttgcca acgctttttc gcggacgttg gcgcgcgaag aaggcggcga
    10981 gccgcccaag cgcgagggcg agagcatcgc cgtaatcgaa gggccgaccg gcgtgggcaa
    11041 atcgcttgcc tacctgctgg cgggcggcat catggcgcag acgcgcggca agcggctgat
    11101 tgtgagcagc gcaacggttg ccttgcagga gcagttggta gaccgcgacc tgccgttttt
    11161 ggtcgaaaaa agcggtttgg aactgagctt cgcgcttgcc aaagggcgcg gccgctatct
    11221 ctgcccctac aaactctatc aactgacgca aagcaatgcc cagcaaaacc tgctcggctt
    11281 tgaagccccc gccgtcttgt gggacagcaa acccaagccc gaagaattga agctgctgcg
    11341 cgacattgcc gacgaatttt ctgcccgacg gttcaatggc gaccgcgacg cctggccgga
    11401 aaaaatcgac gacgcgattt ggctcaaagt gaccaacgac cgccacggct gcctgaaaac
    11461 cgcctgtccc aaccgtccgg aatgtccgtt ttacctggca cgcgatgtct tggaaaccgt
    11521 cgatgtcgtc gttgccaacc acgatcttct gcttgccgac atcagtatgg gcggcggcgt
    11581 gattctgcct gcgcccgaaa acagtttcta ttgcatagac gaagcgcacc acctgcccaa
    11641 aaaagccctc agccgttttg ccgccgaaca ttcatggaat attgccgttt ggacgctgga
    11701 aaaactgccg cagctgaccg gcaaaattgc cgcgcttacc gataaagccg aacttgccaa
    11761 cctggccgac gaagccgccg catccttgct cgacagcctg cacgaatggc agttccattt
    11821 ggcggaagag ccgtctttaa gtatggggcc gtctgaaaac gacagacgaa ccaacagcga
    11881 accgacttgg ctgtgggaag acggcaaaat ccccgaaggc ctcgaaacca ccgtttccaa
    11941 tacggccatt gctgcgcgca gcctgctcaa acacgttgtc gggctgaacg atgcgctttc
    12001 cgctgcgcgc cgcgaaaaag aacaagacag cgcgctcctc gaccgcctga ccggcgagtt
    12061 cggtcttttt atcgcccgta tcgaacaaat cagcgcggtt tgggatttgc tctccaccgt
    12121 ccccctcgaa ggtgaagaac cgctggcaaa atggataacc cgccgcgccg acgacaaaaa
    12181 cgactacatt ttcaacgcca gccccatcag cagcgcgtcc cacctcgcca acagtctgtg
    12241 gcggcgcgcg gcaggcgcgg tgttgacctc cgccaccctg caatccttgg gcaactttaa
    12301 cctgatattg cgccaaaccg ggctgcaatg gctgcccgaa accaccaccc tcgccctcaa
    12361 aagccccttt gactttgaaa aacagggcga actctacatc ccccccatat acgccagccc
    12421 caaagacccc gaagcccaca ccgccgccat catcgaatgg ctgcccaagc ttatttcgcc
    12481 caccgaagcc atcggcacgc tcgtcctgtt ttcctcgcgc aaacaaatgc aggatgtcgc
    12541 cctgcgcctg cccggagact acctgccgct cttgctcgta caaggcgaat tacccaaagc
    12601 cgtcctcctg caaaaacacc accgggccat agaagaaggc aaagccagca tcatcttcgg
    12661 actcgacagt ttcgccgaag gactcgacct gcccggcacc gcctgcgtgc aagtcatcat
    12721 cgccaagctg ccgtttgcga tgcctgacaa ccccatcgaa aaaacccaaa accgctggat
    12781 agaacagcgc ggcggcaatc cttttattga aatcaccgtc cccgaagccg gcatcaaact
    12841 catccaggcc gtcggccgcc tcatccgcac cgaacaagac tacggccgcg taaccatcct
    12901 cgacaaccgc atcaaaacac agcggtacgg ccaacaatta ttggccggcc tgccgccgtt
    12961 taaaaggata gggtaaacat accgcccttc ccgcaacaga aaggaaaaat tcatgaactt
    13021 tacccgactg ctcaaccaag tcttaagcac ggttcaaaaa aaaggcaaca cattctccga
    13081 cagcccgctc aattcattcg gcggaggcgc gctggttgcc ggtatcgcct ccatgctgct
    13141 gaacggtaaa aaccgcaaaa ccattaccaa aatcggttcg accgccgctt tgggctacct
    13201 cgcctaccgg ggctatcaga tgtggcagca aaacaaaggg cgggcaactg taacacaaag
    13261 cgatttccaa cctgccggag aaactgaaga aacatacagc cgcaccgtat tgcgcaccat
    13321 gatagccgcc gccgcttcag acggcatgat agacgaagcc aaacgccgga ctatcgaaca
    13381 ggaaagcggc acagaccccg aaactgccgc atggctcgcc gccgaatacc gcctgcccgc
    13441 aagcatcgaa gacatcgccg ccgccgtcgg caacgatgag gcgttggcgg cggaggccta
    13501 tctggcggca aggttggtct gtgccgattt gtcgcggaaa gaaaccgtct tcctcgcccg
    13561 cctgtcgcag gctttgaaac tggatgacaa tctggtggag agtttggaaa ggcaattggg
    13621 gttttgatga cacagccggg gagggaaaat catcaaaaaa cggttattcc gaccccggcc
    13681 tcaaaacgg
//
LOCUS       DAWFHY010000055        13632 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.055, whole genome shotgun sequence.
ACCESSION   DAWFHY010000055 DAWFHY010000000
VERSION     DAWFHY010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13632
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.055"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..80)
                     /locus_tag="WGQ68_001400"
     CDS             complement(<1..80)
                     /locus_tag="WGQ68_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-fructose-6-phosphate
                     aminotransferase"
                     /protein_id="HEZ0715182.1"
                     /translation="MKPAVWAALLLCACTSNFGDREHQFLR"
     gene            283..1611
                     /gene="miaB"
                     /locus_tag="WGQ68_001401"
     CDS             283..1611
                     /gene="miaB"
                     /locus_tag="WGQ68_001401"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEZ0715183.1"
                     /translation="MKKVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADII
                     LFNTCSVREKAQEKVFSDLGRVRPLKEKKPGLIIGVAGCVASQEGENIVKRAPYVDVV
                     FGPQTLHRLPKMIVDKETSGLSQVDISFPEIEKFDHLPPARVEGGAAFVSIMEGCSKY
                     CSFCVVPYTRGEEFSRPLNDVLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDF
                     ATLLRIVHEIPGIERMRFTTSHPREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSA
                     MKRGYTALEYKSIIRKLRAIRPDLCLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSF
                     VFIYSPRPGTPAANLHDDTPHEEKVRRLEALNEVIEAETARINQTMIGTVQRCLVEGI
                     SKKDPDQLQARTANNRVVNFTGTPNMINQMIDLEITEAYTFSLRGKVVEA"
     gene            complement(1696..3609)
                     /gene="dxs"
                     /locus_tag="WGQ68_001402"
     CDS             complement(1696..3609)
                     /gene="dxs"
                     /locus_tag="WGQ68_001402"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEZ0715184.1"
                     /translation="MNPSPLLDLIDSPQDLRRLDKKQLPRLAGELRAFLLESVGQTGG
                     HFASNLGAVELTIALHYVYDTPEDKLVWDVGHQSYPHKILTGRKNQMHTMRQYGGLAG
                     FPKRSESEYDAFGVGHSSTSIGAALGMAAADKLLGSDRRSVAIIGDGAMTAGQAFEAL
                     NCAGDMDVDLLVVLNDNEMSISPNVGALPKYLASNVVRDMHGLLSTVKAQTGKVLDKI
                     PGAMEFAQKVEHKIKTLAEEAEHAKQSLSLFENFGFRYTGPVDGHNVENLVDVLKDLR
                     SRKGPQLLHVITKKGNGYKLAENDPVKYHAVAKLPKEGAAQMPSEKEPKPVAKPTYTQ
                     VFGKWLCDRAAADERLIAITPAMREGSGLVEFEQRFPDRYFDVGIAEQHAVTFAGGLA
                     CEGMKPVVAIYSTFLQRAYDQLVHDIALQNLPVLFAVDRAGIVGADGPTHAGLYDLSF
                     LRCIPNMIVAAPSDENECRLLLSTCYQADAPAAVRYPRGTGTGAPVSDGMETVEIGKG
                     IIRREGGKTAFIAFGSMVAPALAVAGKLNATVVDMRFVKPIDEELIVRLARSHDRIVT
                     LEENAEQGGAGGAVLEVLAKHGICKPVLLLGVADTVTGHGDPKKLLDGLGLSAEAVER
                     RVRAWLSDRDAAN"
     gene            complement(3694..4611)
                     /gene="xerC"
                     /locus_tag="WGQ68_001403"
     CDS             complement(3694..4611)
                     /gene="xerC"
                     /locus_tag="WGQ68_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEZ0715185.1"
                     /translation="MVLDGFAAHFDAYLENIVREGKSEHTVAAYRRDLEELFALLAQM
                     PSEAEGGVPQGLSQRDFTAALRRLSQRGLNARTLARKLSSWRQYCAWLVKRGLLHTDP
                     TADIKPPKQPERVPKALPQEWLNRMLDLPVDGGDSLAVRDHALFELMYGSGLRVSEIH
                     GLNADDVYLDEAWVHVTGKGRKQRQVPLTGKSVEALKNYLPLRQTASDGKALFTGRNG
                     TRLSQRQIQKRLAQWAAQNGDGRHVSPHMMRHSYAGHLLQASRDIRAVQELLGHSSLS
                     TTQIYTKLDFDHIARLYDEAHPRAKRQDE"
     gene            4805..5869
                     /locus_tag="WGQ68_001404"
     CDS             4805..5869
                     /locus_tag="WGQ68_001404"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase class II"
                     /protein_id="HEZ0715186.1"
                     /translation="MALVSMRQLLDHAAENSYGLPAFNVNNLEQMRAIMEAADQVNAP
                     VIVQASAGARKYAGAPFLRHLILAAVEEFPHIPVVMHQDHGASPDVCQRSIQLGFSSV
                     MMDGSLLEDGKTPSSYEYNVNATRTVVNFSHACGVSVEGEIGVLGNLETGEAGEEDGV
                     GAVGKLSHDQMLTSVEDAVRFVKDTGVDALAIAVGTSHGAYKFTRPPTGDVLRIDRIK
                     EIHQALPNTHIVMHGSSSVPQEWLKVINEYGGNIGETYGVPVEEIVEGIKHGVRKVNI
                     DTDLRLASTGAVRRYLAENPSDFDPRKYLSKTIEAMKQICLDRYLAFGCEGQAGKIKP
                     VSLEKMANRYAKGELNQIVK"
     gene            5889..6857
                     /locus_tag="WGQ68_001405"
     CDS             5889..6857
                     /locus_tag="WGQ68_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="factor H binding family protein"
                     /protein_id="HEZ0715187.1"
                     /translation="MPSELPFGRYLIFASLTCLIDAICKKRYRNQNVYILSILCMTRS
                     KPVNRTAFCCLFLTTALILTACSSGGGGVAADIGTGLADALTAPLDHKDKGLKSLTLE
                     DSIPQNGTLTLSAQGAEKTFKAGDKDNSLNTGKLKNDKISRFDFVQKIEVDGQTITLA
                     SGEFQIYKQDHSAVVALQIEKINNPDKIDSLINQRSFLVSGLGGEHTAFNQLHGGKAE
                     YHGKAFSSDDAGGKLTYTIDFAAKQGHGKIEHLKTPEQNVELAAAELKADEKSHAVIL
                     GDTRYGSEEKGTYHLALFGDRAQEIAGSATVKIGEKVHEIGIAGKQ"
     gene            6950..7627
                     /gene="tsaB"
                     /locus_tag="WGQ68_001406"
     CDS             6950..7627
                     /gene="tsaB"
                     /locus_tag="WGQ68_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEZ0715188.1"
                     /translation="MQADFNRPILAIDTGTSHLSLALRADGETRLFHQEAGSRQSEWI
                     LPQIRTLFHDAGITAADLGAIVYAKGPGAFTGLRIGIGVAQGLATPFNTPLIGIPTLD
                     AAASLPPPQSCILAATDARMGEVFYAWFDTLNCRRLSDYQVGLAADIRLPEGCAFSDG
                     IGSAFALENRPPFAGKPDMPTAADFLNLAADGGFPAVAAAHAELLYVRDKIALTAKEQ
                     AERRARP"
     gene            7624..8064
                     /gene="rimI"
                     /locus_tag="WGQ68_001407"
     CDS             7624..8064
                     /gene="rimI"
                     /locus_tag="WGQ68_001407"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEZ0715189.1"
                     /translation="MNIRRAVYADCAALAALDAVCNPSAWTQRQFESALVSPSEQVFL
                     AEKDGGIAAFIVWQNLPDESELHLIATALEYRRRGVASALLEYWFAHLPEGTKRLLLE
                     VRAGNIGAQALYAAHGFTDAGRRKNYYRTADGKTEDAVLMEKIC"
     gene            8058..8789
                     /locus_tag="WGQ68_001408"
     CDS             8058..8789
                     /locus_tag="WGQ68_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010791194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEZ0715190.1"
                     /translation="MLSARYLHLHEALGLGPMWLKRGAVVLPPATLPGSLRPQKQTVL
                     SIPQRPSEQHTGQARLKTMKVLETAAVHTRKPVPETETPLPGVSDGIAPVSAASGITK
                     LAVVSLCPPTEDMVYGQLFHGKAGVLLDNILKAVGLDAAYVHKTSWVKTAAVGSPMPS
                     EQAIANALGQIARELDGCRAPAVLFLGQAFVQPERQTMIETLCGSRPFFIIDHPARLL
                     RQPELKARNWQVLKQLKHALQAGGS"
     gene            8851..9492
                     /gene="pyrE"
                     /locus_tag="WGQ68_001409"
     CDS             8851..9492
                     /gene="pyrE"
                     /locus_tag="WGQ68_001409"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEZ0715191.1"
                     /translation="MTDFRQDFLKFSLAQNVLKFGEFTTKAGRQSPYFFNAGLFNDGL
                     STLQLAKFYAQSIIESGIQFDMLFGPAYKGIILAAATAMMLAEKGVNVPFAYNRKEAK
                     DHGEGGVLVGAPLKGRVLIIDDVISAGTSVRESIKLIEAEGATPAGVAIALDRMEKGT
                     GELSAVQEVEKQYGLPVAPIASLNDLFILLQNNPEFGQFLEPVRAYRRQYGVE"
     gene            9566..10000
                     /locus_tag="WGQ68_001410"
     CDS             9566..10000
                     /locus_tag="WGQ68_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715192.1"
                     /translation="MPACFCPHCKTRLWVKETQLNVAQGFVVCQKCEGLFKAKDHLAS
                     TKEPIFNDLPEAVSDVKLVHRIGTHAIGKKQISRDEIAGILNGGTTLHDTPPAPAAAP
                     AAAPQVAVPPAAPARQEGFNWTIATLFALIVLIMQLSYLFIL"
     gene            9997..11307
                     /gene="argA"
                     /locus_tag="WGQ68_001411"
     CDS             9997..11307
                     /gene="argA"
                     /locus_tag="WGQ68_001411"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HEZ0715193.1"
                     /translation="MNAPDSFVAHFREAAPYIRQMRGTTLVAGIDDRLLEGDTLNKLA
                     ADIGLLSQLGIRLVLIHGAHPFPDRPAAGQNRTPHYCRGLRVTDETSLEQAQQFAGTV
                     LSRFEAALCGSVSGFARAPSVPLVSGNFLTARPVGIIDGVDMEYTGVIRKTDTAALRF
                     QLDAGNIVWLPPLGHSYGGKTFHLDMLQTAAAVAVSLQAEKLVYLTLSDGIARPDGTL
                     AETLSAQEAQSLAEHAGSETQRLVASALTALEGGVHRVQILNGAADGSLLQELFTRNG
                     IGTSIAKEAFVSIRQAHSGDIPHIAALIRPLEEQGILLHRSREYLENHISEFSILEHD
                     GNLYGCAALKTFAEADCGEIACLAVSPQAQDGGYGERLLAHIIDKARGIGISRLFALS
                     TNTGEWFAERGFQTASEAELPETRRKDYRSNGRNSHILVRRLHR"
     gene            11403..13418
                     /locus_tag="WGQ68_001412"
     CDS             11403..13418
                     /locus_tag="WGQ68_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEZ0715194.1"
                     /translation="MKSYPDPYRHFENLDSAETQNFAAEANAETRARFLENDKARALS
                     DGILAQLQDTRQIPFCQEHRARMYHFHQDAEYPKGVYRVCTAATYRSGYPEWKILFSV
                     ADFDELLGDDVYLGGVSHLVEQPDRALLTLSKSGGDTAYTLEVDLEAGELVEGGFHFP
                     AGKNHVSWRDENSVWVCPAWDERQLTESGYPREVWLVERGKSFEESLPVYQIAEDGMM
                     VNAWRYLDPQGSPIDLIEASDGFYTKTYLQVSAEGEAKPLNLPADCDVAGYLAGHLLL
                     TLRKDWNRTNQSYPSGALVAVKLNRGEIGAAQLLFAPDETQALESVETTKRFVVASLL
                     ENVQGRLKAWRFADGKWQEAELPRLPSGALEMTDQPWGGDVVYLAAGDFTTPLTLFAL
                     DLNVMELTVMRRQPQQFVSDGINVQQFWTTSADGERIPYFHVGKNAAPDTPTLVYAYG
                     GFGVPELPHYLGSIGKYWLEEGNAFVLANIRGGGEFGPRWHQAAQGISKHKSVDDLLA
                     VVRDLSERGISSPEHIGLQGGSNGGLVTAAAFVREPQSIGALVCEVPLTDMIRYPLLS
                     AGASWTDEYGNPQKYEVCKRWLGELSPYHNLSDGIDYPPALITTSLSDDRVHPAHALK
                     FYAKLRETSPQSWLYSPDGGGHTGNGTQRESADEMACVLLFLKEFLG"
     gene            complement(13510..>13632)
                     /locus_tag="WGQ68_001413"
     CDS             complement(13510..>13632)
                     /locus_tag="WGQ68_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEZ0715195.1"
                     /translation="NISNREIRRVQDELNHRPRKTLGYETPSVLFLNLFQPLIH"
BASE COUNT         3061 a         4221 c         3639 g         2711 t
ORIGIN      
        1 cgcaggaact gatgttccct gtcgccgaaa ttgctggtac acgcacacag cagcaatgcc
       61 gcccacacag ccggtttcat acacacctcc cattaaagcc aaacattata cagccgttct
      121 gaccgattaa attcatattt taaaacaata ttctgtttac aaaacccaca tcgtgctata
      181 atccgcaccg attttcagac ggcatcgtca tgccgtctga aattttttca ttccaacaac
      241 aatcagcccc gcgattacgg ctgcctgaga aagacacaaa ccatgaaaaa agtatttatc
      301 cgcaccttcg gctgccagat gaacgaatac gacagcgaca aaatgctcgc cgtcctcgcc
      361 gaagaacacg gcggcatcga acaggttacc caagccgacg aagccgacat catcttgttc
      421 aacacctgct ccgtgcgcga aaaagcgcaa gaaaaagtct tctccgattt ggggcgcgtg
      481 cgtccgctca aagaaaaaaa acccggcctc atcatcggcg ttgccggttg cgtcgcctcg
      541 caagaaggcg aaaacatcgt caaacgcgcg ccgtatgtgg acgtggtttt cggcccgcaa
      601 acgctgcacc gcctgcccaa aatgattgtg gacaaagaaa ccagcgggct gtcgcaagtc
      661 gatatttcct ttcccgaaat cgaaaaattc gaccacctgc cgcccgcccg cgtcgaaggc
      721 ggcgcggcat ttgtatcgat tatggaaggc tgttccaaat actgctcctt ctgcgtcgtc
      781 ccctacacgc gcggcgaaga attctcccgc ccgctcaacg acgtattgac agaaatcgcc
      841 aaccttgccc agcaaggcgt gaaagaaatc aacctcttgg gacaaaacgt caacgcctat
      901 tgcggcgaaa tggacgacgg cgaaatctgc gacttcgcca ccctgctgcg catcgtccac
      961 gaaatccccg gcatcgaacg tatgcgcttc accaccagcc acccgcgcga gtttaccgac
     1021 tcgattatcg agtgctaccg cgacctgccc aaattggttt cccacctgca cctgccgatt
     1081 caaagcggtt ccgaccgcgt attgagcgcg atgaaacgcg gctacaccgc tttggaatac
     1141 aaatccatca tccgcaaact gcgtgccatc cgtcctgatt tatgcctgag cagcgatttc
     1201 atcgtcggct tccccggcga gaccgaacgc gagttcgagc aaaccttgaa actggtgaaa
     1261 gacattgcct tcgacttgag cttcgtgttt atttacagtc cgcgccccgg cacgcctgcc
     1321 gccaacctgc acgacgacac gccgcacgaa gaaaaagtgc gccgcctcga agccttgaac
     1381 gaagtcatcg aagccgaaac cgcgcgcatc aaccaaacga tgatcggcac ggttcaacgc
     1441 tgcctggtcg aaggcatctc caaaaaagac cccgaccagc tccaagcccg caccgccaac
     1501 aaccgcgtcg tcaactttac cggcacgccc aacatgatta accaaatgat cgatttggaa
     1561 atcaccgagg cctacacctt ctccctgcgc ggcaaagttg tcgaagccta aacccttacg
     1621 ccgaaaaaat gccgtctgaa gcgtttcaga cggcattttg ccttgtatcg gcagacgatg
     1681 gcgcggccag gcggcttaat ttgccgcatc ccgatccgac agccacgcgc gcacacgccg
     1741 ttccaccgct tcggcactca aacccaaacc gtccaaaagt tttttcggat cgccgtgtcc
     1801 ggttacggta tcggcaacgc ccaaaagcaa aacgggtttg cagatgccgt gtttcgccaa
     1861 tacttccagc accgcgccgc ctgcgccgcc ctgttcggcg ttttcttcaa gggtaacgat
     1921 gcggtcgtgg cttcgggcaa ggcggacaat caactcttcg tctatcggtt tgacaaagcg
     1981 catatcgacg acggtggcgt tcagttttcc ggcgaccgcc aatgcggggg cgaccatact
     2041 gccgaaggca atgaatgcgg ttttcccacc ttcgcggcgg ataatgccct tgccgatttc
     2101 cacggtttcc atgccgtctg aaaccggcgc gcccgtaccc gtgccgcgcg gatagcggac
     2161 ggcggcgggc gcatccgcct gatagcaggt cgaaagcagc aggcggcatt cgttttcatc
     2221 gctcggcgcg gcgacaatca tattcggaat gcagcgcaaa aagctcaaat cgtacagacc
     2281 ggcatgggtc gggccgtccg cgccgacgat gcccgcgcgg tcgacggcaa acaaaacggg
     2341 caggttttgc agggcgatgt cgtgcaccag ttggtcgtag gcgcgttgta aaaaggtgga
     2401 ataaatcgcc acgacgggct tcatgccttc gcacgccaaa ccgccggcaa aggtaacggc
     2461 gtgctgctcg gcaatgccga catcgaaata acggtcgggg aatcgttgtt caaactccac
     2521 cagtccgctg ccctcgcgca tcgcgggcgt gatggcgatc aaacgctcgt ctgccgccgc
     2581 ccggtcgcac agccatttgc cgaacacttg ggtataggtc ggtttggcga cgggcttggg
     2641 ttctttttca gacggcattt gcgccgcgcc ttctttaggc agcttggcga cggcgtggta
     2701 tttgacgggg tcgttttcgg cgagtttgta gccgttgcct tttttggtga tgacgtgcag
     2761 aagctgaggg cctttgcggc tgcgcaagtc tttcaatacg tccaccagat tttcgacgtt
     2821 gtgtccgtcc acggggccgg tgtagcggaa gccgaagttt tcaaacaaag acagcgactg
     2881 tttggcgtgt tcggcttctt cggcaagggt tttgattttg tgttcgactt tttgggcaaa
     2941 ctccatcgcg ccgggtattt tgtctaatac cttgcccgtt tgcgctttga cggtactcaa
     3001 caggccgtgc atatcgcgca cgacgttgct ggcaaggtat ttcggcagcg cgccgacgtt
     3061 gggggaaatc gacatttcgt tgtcgttgag gacgaccagc aaatccacat ccatatcgcc
     3121 tgcgcaattc aaggcttcaa acgcctgccc cgccgtcatc gcgccgtcgc cgatgatggc
     3181 gacgctgcgg cggtcgctgc ccaagagttt gtctgccgcc gccatgccca acgccgcgcc
     3241 gatggatgtg gaagaatgcc ctacgccgaa cgcgtcgtat tccgactcgc tgcgttttgg
     3301 gaagccagct aaaccgccgt attggcgcat ggtgtgcatc tggtttttcc tgcctgtcag
     3361 gattttgtgc ggatagcttt ggtgtccgac atcccatacc agcttgtctt cgggcgtatc
     3421 gtacacatag tgcagggcga tggtcagttc gaccgcgccc aaattgctgg cgaaatgccc
     3481 gccggtctgc ccgacagatt ccagcagaaa ggcgcgcaac tcgccggcaa ggcgcggcag
     3541 ctgttttttg tccagacggc gcaaatcttg cgggctgtca atcaggtcga gtagggggct
     3601 tgggttcatg gtgtgtcttt tttatgtgtc gtccgggtgc aacggccaat tatatatcaa
     3661 gagcgtgcgg ctgacggctg attttgccgt atgtcattcg tcctgccgct tggcgcgcgg
     3721 gtgggcttcg tcatacaggc gggcgatgtg gtcgaaatcg agcttggtat aaatctgcgt
     3781 ggtcgaaagg ctgctgtgcc cgagcagctc ctgcaccgcc ctgatgtcgc gcgaagcctg
     3841 caacaggtgt ccggcgtagc tgtggcgcat catatgcggc gaaacgtgcc tgccgtcgcc
     3901 gttttgcgcc gcccattgcg ccaaacgttt ttggatttgg cgttggctca ggcgcgtgcc
     3961 gttcctgccg gtaaacaggg ctttgccgtc cgatgccgtc tgacgcagcg gcagatagtt
     4021 tttcagggct tccacgcttt tgccggtcag cggcacctgc cgctgcttgc gccctttgcc
     4081 ggtaacgtgt acccacgcct cgtccaaata gacatcatct gcattcaagc cgtgtatctc
     4141 gctcacgcgc aaaccgctgc cgtacatcag ttcgaacagc gcgtgatcgc gcaccgccag
     4201 cgagtcgccg ccgtccacgg gcaaatccag catccggttc agccattcct gcggcagggc
     4261 tttgggtacg cgctcgggct gcttcggcgg tttgatgtcg gcggtcgggt cggtatgcag
     4321 cagaccgcgt ttgaccagcc aggcgcaata ctgccgccaa gacgacagct tgcgcgccag
     4381 cgtccgcgca ttcaaaccgc gctgcgacag ccgccgcaac gccgccgtaa aatcgcgttg
     4441 cgacaagccc tgcggcacgc cgccttcagc ttcagacggc atttgtgcca acagcgcaaa
     4501 cagttcttcc aaatcgcgcc ggtatgccgc caccgtgtgc tccgacttgc cctcgcgcac
     4561 gatgttttcc aaataagcgt caaaatgcgc cgcaaacccg tccaaaacca taaccgcccc
     4621 acacaaatat caaaaaaaca gtgaaaacat tatcataaac gacacaccaa aaccccgaac
     4681 cgcccaaaat ccgacccgtc cgcccgccgt tatcgttaac ataaaatcaa tgagccgttt
     4741 atggtttttt gccgcaaaaa acattataat ccgccttatt tacctattgc ccaaggagac
     4801 acaaatggca ctcgtatcca tgcgccaact gcttgatcat gctgccgaaa acagctacgg
     4861 cctgcccgcg ttcaacgtca acaacctcga acaaatgcgc gccattatgg aagccgccga
     4921 ccaagtcaac gcgcccgtca tcgtacaggc gagtgcaggt gcgcgcaaat atgcgggcgc
     4981 gccgtttttg cgccacctga ttctggcggc tgtcgaagaa tttccgcaca tccccgtcgt
     5041 gatgcaccaa gaccacggcg catcgcccga cgtgtgccaa cgctccatcc aactgggctt
     5101 ctcctccgtg atgatggacg gctcgctgct cgaagacggc aaaacccctt cttcttacga
     5161 atacaacgtc aacgccaccc gtaccgtggt taatttctcc cacgcctgcg gcgtatccgt
     5221 tgaaggcgaa atcggcgtat tgggcaacct cgaaaccggc gaagcaggcg aagaagacgg
     5281 tgtaggcgca gtgggcaaac tttcgcacga ccaaatgctg accagcgtcg aagatgccgt
     5341 acgtttcgtt aaagataccg gcgttgacgc attggctatt gccgtcggta ccagccacgg
     5401 cgcgtacaaa ttcacccgtc cgcccacagg cgacgtattg cgtatcgacc gcatcaaaga
     5461 aatccaccaa gccctgccca atacacacat cgtgatgcac ggctccagct ccgttccgca
     5521 agaatggctg aaagtcatca acgaatacgg cggcaatatc ggcgaaacct acggcgtgcc
     5581 ggttgaagaa atcgtcgaag gcatcaaaca cggcgttcgt aaagtcaaca tcgataccga
     5641 cctgcgcctt gcttccaccg gtgcggtacg ccgctacctt gccgaaaatc cgtccgactt
     5701 cgatccgcgc aaatacctga gcaaaaccat tgaggccatg aagcaaatct gcctcgaccg
     5761 ctacctcgcg ttcggttgcg aaggtcaggc aggcaaaatc aaacctgttt cgttggaaaa
     5821 aatggcaaac cgttatgcca agggcgaatt gaaccaaatc gtcaaataac aggttgccgg
     5881 taaacaaaat accgtctgaa ctgccgttcg gacggtattt gatttttgct tctttgacct
     5941 gcctcattga tgcaatatgc aaaaaaagat accgcaacca aaacgtttat atattatcta
     6001 ttctgtgtat gactaggagt aaacctgtga accgaactgc cttctgctgc cttttcctga
     6061 ccaccgccct gattctgacc gcctgcagca gcggaggggg tggtgtcgcc gccgacatcg
     6121 gcacggggct tgccgatgca ctaactgcgc cgctcgacca taaagacaaa ggtttgaaat
     6181 ccctgacatt ggaagactct attccccaaa acggaacact gaccctgtcg gcacaaggtg
     6241 cggaaaaaac tttcaaagcc ggcgacaaag acaacagcct caacacgggc aaactgaaga
     6301 acgacaaaat cagccgcttc gatttcgtgc aaaaaatcga agtggacgga caaaccatca
     6361 cgctggcaag cggcgaattt caaatataca aacaggacca ctccgccgtc gttgccctac
     6421 agattgaaaa aatcaacaac cccgacaaaa tcgacagcct gataaaccaa cgctccttcc
     6481 ttgtcagcgg tttgggcgga gaacataccg ccttcaacca actgcacggc ggcaaagccg
     6541 agtatcacgg caaagcattc agctccgacg atgccggcgg aaaactgacc tataccatag
     6601 atttcgccgc caaacaggga cacggcaaaa tcgaacacct gaaaacaccc gagcaaaatg
     6661 tcgagcttgc cgccgccgaa ctcaaagcag atgaaaaatc acacgccgtc attttgggcg
     6721 acacgcgcta cggcagcgaa gaaaaaggca cttaccacct cgcccttttc ggcgaccgcg
     6781 ctcaagaaat cgccggctcg gcaaccgtga agataggaga aaaggttcac gaaatcggca
     6841 tcgccggcaa acagtagcat ttttgcgcca taccgcccta ggacacgacg gcacggcagg
     6901 caagccggtt tcactatata atgccgtctg aaccaacgag aaccacacca tgcaagctga
     6961 ttttaaccgt cccatcctgg ctatcgatac cggtacttcc catttgtcgc tcgcgctgcg
     7021 tgccgacggc gaaacccgcc tgttccatca agaggccggc agccgccagt ccgaatggat
     7081 tttgccgcaa atccgcacgc tatttcacga tgcgggcatt accgccgccg atttgggggc
     7141 gattgtttat gcgaaaggcc ctggcgcgtt tacgggactg cgtatcggca tcggcgtggc
     7201 gcaaggtttg gcaacgccgt tcaatacgcc tttaatcggc atccccacgc tcgatgccgc
     7261 cgcctcgctg ccgccgccgc aaagctgcat ccttgccgct acggacgctc gtatgggcga
     7321 agtgttttat gcgtggttcg acacgctgaa ctgccgccgt ttgagcgatt atcaggtcgg
     7381 gctggcggca gacatccggc tgccggaggg atgtgccttt tcagacggca taggcagcgc
     7441 gttcgcgctg gaaaaccgtc cgcccttcgc aggcaaaccc gatatgccga ctgccgccga
     7501 ttttctcaat ctggcggcgg atggcggttt tcccgccgtc gctgcggcac acgccgagct
     7561 gctctacgtc cgcgacaaaa tcgccctgac tgccaaagaa caggcggaac ggagggcgcg
     7621 tccgtgaaca tccgccgtgc cgtttatgcc gattgcgcgg cactcgccgc actcgatgcc
     7681 gtctgcaacc cgtccgcgtg gacgcaacgc caatttgagt ccgcactcgt ttcgccgtcc
     7741 gaacaggttt ttcttgcgga aaaagacggc gggattgccg cctttatcgt ttggcagaac
     7801 ctgcccgacg aatccgaact gcacctgatt gccaccgctt tggaataccg ccgccggggt
     7861 gtcgcgtccg ccctgctcga atactggttc gcacacctgc ccgaaggcac gaaacgcctg
     7921 ctgcttgaag tccgcgcggg caatatcggc gcgcaggcgc tgtacgcggc acacggcttt
     7981 accgatgcgg gcaggcggaa aaactattac cgtacagccg acggtaaaac cgaagatgcc
     8041 gtcttaatgg agaaaatatg ttaagcgcgc gctacctcca cttgcacgaa gctttgggtt
     8101 tgggcccgat gtggttgaag cggggggccg tcgtcctgcc accagcaaca ttgccgggaa
     8161 gcctacgccc gcaaaagcaa accgtcctta gcattccgca gcgtccgtcc gaacagcata
     8221 ctggtcaggc acggctcaaa accatgaaag tgttggaaac agccgccgta catacgcgca
     8281 aacccgtgcc tgaaaccgaa acgcctctgc ccggcgtttc agacggcatc gcccccgttt
     8341 ccgccgcttc gggcataacc aaacttgccg tcgtcagcct gtgtccgccg accgaggata
     8401 tggtttacgg gcaactgttc cacggcaaag cgggtgtcct gcttgacaac atactcaaag
     8461 ccgtaggact ggatgccgct tatgtacaca agaccagctg ggtgaaaacc gccgccgtcg
     8521 gcagcccgat gccgtctgaa caggccatcg cgaatgcgct gggtcaaatc gccagggaac
     8581 tcgacggctg ccgcgccccg gccgtccttt tcctcgggca ggcttttgtc cagcctgaac
     8641 ggcaaacgat gattgaaact ttgtgcggca gccgtccctt cttcatcatc gatcatcccg
     8701 cccggctttt acgccaaccc gaacttaaag cccgaaactg gcaggtgttg aaacagttga
     8761 aacacgcctt gcaggcaggc ggcagttgaa gcaggccgca cggggcggta gaatcacaac
     8821 tgcatcgcaa tacctgacag aaagcaaaaa atgaccgatt tccgccaaga tttccttaaa
     8881 ttctccctcg cccaaaacgt cttaaaattc ggcgaattta ccaccaaggc aggacggcag
     8941 tcgccttact tcttcaatgc cggcctcttt aacgacggct tgtccacact gcaactggca
     9001 aaattctatg cacaatccat cattgaaagc ggcatccaat tcgatatgct gttcggcccc
     9061 gcctacaaag gcattatttt ggcggcggca accgcgatga tgctggcgga aaaaggcgtg
     9121 aacgtcccgt tcgcctacaa ccgcaaagaa gccaaagacc acggcgaagg cggcgtgttg
     9181 gtcggtgcgc cgcttaaagg gcgcgtgctg attatcgacg atgtgatttc cgccggcaca
     9241 tccgtacgcg aatcaatcaa actgattgaa gcggagggtg caacccccgc cggcgtcgcc
     9301 atcgcgctcg accgcatgga aaaaggcacc ggcgaattga gcgcggttca ggaagtggaa
     9361 aaacaatacg gcctgcccgt cgcccccatc gccagcctga acgatttgtt tatcctgttg
     9421 caaaacaacc ccgaattcgg acagttcctc gaacccgtcc gagcctaccg tcggcagtac
     9481 ggcgtagaat aaaaacaaag catatgccgt ctgaaccgcc ttacgcctca gacggcatca
     9541 aacctgacac acacgaggaa ataccatgcc cgcctgtttc tgcccccact gcaaaacccg
     9601 tctctgggtc aaagaaaccc aactcaatgt cgcccaaggc ttcgtcgtct gccaaaaatg
     9661 cgaagggctg tttaaagcca aagaccatct ggcaagcacg aaagaaccta tattcaacga
     9721 tttgcccgaa gctgtttcgg atgtcaaact cgtccaccgc atcggcacgc acgccatcgg
     9781 caagaaacag atttcccgcg acgaaatcgc cggcatcctc aacggcggta caaccctgca
     9841 cgatacgccg cctgcgcctg ccgccgcccc tgctgccgca ccgcaggttg ccgtaccacc
     9901 cgccgcgccc gcccgtcagg aagggttcaa ctggacgatt gcaaccctgt tcgcacttat
     9961 cgtcctcatc atgcagcttt cctacctctt catcctatga acgcgcccga cagctttgtc
    10021 gcccacttcc gcgaagccgc cccctatatc cgccaaatgc gcggcacgac actggtcgcc
    10081 ggcatagacg accgcctgct cgaaggcgat accttaaaca agctcgccgc cgacatcggg
    10141 ctgttgtcgc aactgggcat ccgcctcgtc ctcatccacg gcgcgcaccc cttccccgac
    10201 cgccccgccg ccggacaaaa ccgcacgccg cattattgcc ggggcttgcg cgttaccgac
    10261 gaaacctcgc tcgaacaggc gcagcagttt gccggcaccg tcctcagccg ttttgaagcc
    10321 gcattgtgcg gcagcgtttc cgggttcgcg cgcgcccctt ccgtcccgct cgtatcgggc
    10381 aacttcctga ccgcccgccc cgtcggcatc atcgacggcg tagatatgga atacacgggc
    10441 gttatccgca aaaccgacac cgccgccctc cgtttccaac tcgacgcggg aaatatcgtc
    10501 tggctgccgc cgctcggaca ttcctacggc ggcaaaacct tccatctcga tatgcttcaa
    10561 accgccgctg ccgtcgccgt ctcgcttcag gcagaaaaac tcgtttacct gaccctttca
    10621 gacggcattg cccgccccga cggcacgctc gccgaaaccc tctcggcaca ggaagcgcaa
    10681 tcgctggcgg aacacgccgg cagcgaaacg caacggctgg ttgcgtccgc ccttaccgcg
    10741 ctcgaaggcg gcgtgcatcg tgtccaaatc ctcaacgggg ccgccgacgg cagcctgctg
    10801 caagaactct tcacccgcaa cggcatcggc acgtccattg ccaaagaagc cttcgtctcc
    10861 atccggcagg cgcacagcgg cgacatcccg cacattgccg ccctcatccg tccgctggaa
    10921 gaacagggca tcctgctgca ccgcagccgc gaatacctcg aaaaccacat ttccgaattt
    10981 tccatcctcg aacacgacgg caacctgtac ggttgcgccg ccctgaaaac ctttgccgaa
    11041 gccgattgcg gagaaatcgc ctgccttgcc gtctcgccgc aggcacagga cggcggctac
    11101 ggcgaacgcc tgcttgccca cattatcgat aaggcgcgcg gcataggcat aagcaggctg
    11161 ttcgcactgt ccacaaatac cggcgaatgg tttgccgaac gcggctttca gacggcatcg
    11221 gaagccgagt tgcccgaaac gcggcgcaaa gactaccgca gcaacggacg aaactcgcat
    11281 attctggtgc gtcgcctgca ccgctgaccg caacggaaag ccgccgcaga aatgccgtct
    11341 gaaccctgtt tcagacggta tttccccgat tataaagacc tgcccaaccc tcaaggaccc
    11401 cgatgaaatc ctaccccgac ccctaccgcc attttgaaaa cctcgattcc gccgaaacgc
    11461 aaaacttcgc tgctgaagcg aatgccgaaa cgcgcgcgcg ttttttggaa aacgacaagg
    11521 cgcgcgcgct ttcagacggc attttggcgc agttgcagga cacgcggcag attccgtttt
    11581 gtcaggaaca ccgcgcgcgg atgtaccatt tccatcagga cgcggaatat ccgaagggcg
    11641 tgtaccgcgt gtgtacggcg gcgacctacc gttccggcta tcccgagtgg aaaatcctgt
    11701 tttcagtggc ggatttcgac gaactgctcg gcgacgatgt gtatctgggt ggcgtgtcgc
    11761 acttggtgga acagcccgac cgcgcgttgt taacgctgag caaatcgggc ggcgatacgg
    11821 cgtacacgtt ggaagtggat ttggaagcag gggagttggt agaaggcggt tttcactttc
    11881 cggcaggcaa aaaccatgtg tcgtggcgcg atgaaaacag cgtgtgggtg tgtccggctt
    11941 gggacgaacg gcagttgacc gaatcgggct atccgcgcga ggtgtggctg gtggaacgcg
    12001 gcaaaagttt cgaggaaagc ctgcccgtgt atcaaattgc cgaagacggg atgatggtga
    12061 acgcgtggcg ttacctcgat ccgcaaggtt cgccgattga tttgattgaa gcgtcggacg
    12121 gtttttacac caaaacctat ttgcaggtgt cggccgaagg cgaggcgaaa ccgttaaacc
    12181 tgcccgccga ttgcgacgtg gccggctatc tggcggggca tcttttgctg acgctgcgca
    12241 aggactggaa tcgcacgaac caaagctatc cgagcggcgc gctggtggcg gtgaaactga
    12301 atcgcggcga aatcggggcg gcgcagcttt tgtttgcgcc cgatgaaacg caggcattgg
    12361 aaagcgtgga aacgaccaag cgttttgtgg tggcgagcct gctggaaaac gtacaaggcc
    12421 gtctgaaagc gtggcgtttt gccgacggca aatggcagga agccgaactg ccgcgcctgc
    12481 cttcgggcgc gttggaaatg accgaccagc cttggggcgg cgacgtggtt taccttgccg
    12541 ccggcgattt caccacgccg ctgacgctgt ttgcgctgga tttgaacgtg atggaactga
    12601 ccgttatgcg ccgccagccg cagcagtttg tttcagacgg catcaacgtg caacagtttt
    12661 ggacgacttc ggccgacggc gagcgcattc cttatttcca cgtcggcaaa aacgccgcgc
    12721 ccgacacgcc gacgctggtc tatgcctacg gcggtttcgg cgtgcccgag ctgccgcact
    12781 atctgggcag catcggcaaa tactggctgg aagagggcaa cgccttcgtg ttggcgaaca
    12841 tacgcggcgg cggcgagttc ggcccgcgct ggcatcaggc ggcgcaggga atcagcaaac
    12901 acaaaagcgt cgatgatttg cttgccgtcg tgcgcgattt gtccgaacgc ggcatcagtt
    12961 cgcccgaaca catcggtttg cagggcggca gcaacggcgg ccttgttacc gccgccgcct
    13021 tcgtgcgcga accgcaaagc atcggcgcgc tggtgtgcga agtaccgctg accgacatga
    13081 tccgctatcc gctgctctct gccggcgcaa gctggacgga cgaatacggc aacccgcaga
    13141 aatacgaagt ctgcaaacgc tggcttggcg aattgtcgcc gtatcacaat ctttcagacg
    13201 gcatcgatta tccgcccgca ctcatcacca ccagcctcag cgacgaccgc gtccatcccg
    13261 cccacgcgct caagttctac gccaaactgc gcgaaacctc gccgcaatct tggctctact
    13321 cgcctgacgg cggcggtcat accggtaacg gcacgcaacg cgaatccgcc gacgaaatgg
    13381 cgtgcgtgtt gctgtttttg aaagagtttt tgggctaagg gcggggagcg gcactgccgc
    13441 cgcgaatgaa aaaaggtcgt ctgaaactgc tttttcagac gaccttggat tcggatttca
    13501 agtgcaacac tagtgtatta gtggttggaa cagattcaag aataaaacac ttggcgtttc
    13561 gtagccaagt gtttttcttg gtcggtggtt caactcatct tgaaccctgc gtatctcccg
    13621 attgctgatg tt
//
LOCUS       DAWFHY010000056        13602 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.056, whole genome shotgun sequence.
ACCESSION   DAWFHY010000056 DAWFHY010000000
VERSION     DAWFHY010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.056"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(70..210)
                     /locus_tag="WGQ68_001414"
     CDS             complement(70..210)
                     /locus_tag="WGQ68_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003698491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715196.1"
                     /translation="MPEYRNWQNGLNAKCADGDEYGVAQSDCRTREINAKTKEIQGYL
                     ID"
     gene            complement(255..806)
                     /locus_tag="WGQ68_001415"
     CDS             complement(255..806)
                     /locus_tag="WGQ68_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0715197.1"
                     /translation="MPSERRPFQTAFLSNRKTVGKLAERVQSRYDGLHKFKHICSAAM
                     ALIKEPLDKAKQRNEELEAAEEAAAQEALGREQEAARVSEWEERYKLSRSEFEQFWKG
                     LPQTVQNKLQASQKTWKSGMDKICANNAKAEGETPNGIKVSELACKTVETEARLEELY
                     NRKKALIDEMVREADKKELPKRL"
     gene            complement(820..2466)
                     /gene="pgi"
                     /locus_tag="WGQ68_001416"
     CDS             complement(820..2466)
                     /gene="pgi"
                     /locus_tag="WGQ68_001416"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEZ0715198.1"
                     /translation="MKHFHDLPAWSKLWNHFDDSKTLHMREMFEQDPQRAERYWLQVG
                     GLTLDYSKNRINDETMSLLFELAREAGVPERMRQMFHGEKINTTENRAVLHVALRNRT
                     NSPIVVDGEDVMPKVNRVLQRMGEFAHEVRSGSWLGYTNQVITDVVNIGIGGSDLGPL
                     MMCTALKPFGHPRLNMHFVSNVDGSQLRDVLSKVHPETTLFIIASKTFTTQETLTNAL
                     TAREWFLNHAGDEEAVAKHFAAVSTNQKAVAEFGIDTANMFEFWDWVGGRYSLWSAIG
                     LPIMLYLGEENFIEMLNGAHLMDQHFINTPLERNLPVILALIGIWYINYYGGGSHVIA
                     PYDQHLHRLPKFIQQLDMESNGKQVTLDGKAVGHETSPIIWGETGINGQHAFFQLLHQ
                     GTHITPIDLIASLEKRSNLPGHHEILLANVFAQAEAFMRGKTPDEVRAELKAQGMDEA
                     RIEELVPHKTFSGNRPTNLILMDKVNPRNMGSLIAMYEHKTFVQGIIWGINSFDQWGV
                     ELGKQLAKTILGELTGETGPQKHDSSTERLINLYLQTNRK"
     gene            complement(2587..3435)
                     /gene="hexR"
                     /locus_tag="WGQ68_001417"
     CDS             complement(2587..3435)
                     /gene="hexR"
                     /locus_tag="WGQ68_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HEZ0715199.1"
                     /translation="MLSKISESLANLSGAERKVAECALAEPKWFVHAAVAEIAERASV
                     SQPTVIRFCRSLGYKGLPEFKLALSASIGHEGMPYVHEELNADDDMASVVEKVLGNAA
                     ASLLGERRFLKESELENAIATLMHARRVEFYGVGNSGIVAQDAQHKFFRFGMSTVAYV
                     DTHTQLMAASVLSDQDVLVAISNTGSSIELLDAVSIAKENGASVIALTRNDSPLAQLA
                     DCVLSVATQENAELYTPMVSRLLQLAVIDILAIGLALRLGDAASLQLQKSKKSIHNKH
                     IDYDKD"
     gene            complement(3485..4471)
                     /locus_tag="WGQ68_001418"
     CDS             complement(3485..4471)
                     /locus_tag="WGQ68_001418"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HEZ0715200.1"
                     /translation="MSSTPNKQAGYPRLVADIGGTNARFALETAPRVIEKAAVLPCKD
                     YDTVTDAVRAYLNQSGATAVRHAAFAIANPILGDWVQMTNHHWAFSIETTRQTLGLDT
                     LILLNDFTAQALAVTQTSSKDLMQVGGQKPVEFAPKAVIGPGTGLGVSGLVHSHAGWV
                     ALAGEGGHTSFPPFDDMEVLIWQYAKNKYGHVSAERFLSGAGLSLVYEALAAKQKAKP
                     AKLMPSEITEKALSGASPLCRQTLDIFCAMLGTVASNLALTLGARGGVYLCGGIIPRV
                     LEYFKTSPFRSRFENKGRFEAYLAAIPVYVVLSEFPGISGAAAALDNHLRNV"
     gene            complement(4452..5147)
                     /gene="pgl"
                     /locus_tag="WGQ68_001419"
     CDS             complement(4452..5147)
                     /gene="pgl"
                     /locus_tag="WGQ68_001419"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEZ0715201.1"
                     /translation="MFVWHEYENAAEAAQSLTDAVADALQGALDEKGGAVLAVSGGRS
                     PIAFFNALSQKDLDWKNVGITLADERIVPTVHADSNTGLVREYLLKNKAEAAMWIPMV
                     EDGKTETELHPDAVVDYALKHYKQPDVLVLGMGNDGHTASIFPKAPQFQTAIDGSAGV
                     ALVHTTPVTAPHERVSMTLDAIAHTGHVFLAIQGEEKKAVFEQAAQGENREYPINLVL
                     NHQGVNCHVFYAE"
     gene            complement(5427..6872)
                     /gene="zwf"
                     /locus_tag="WGQ68_001420"
     CDS             complement(5427..6872)
                     /gene="zwf"
                     /locus_tag="WGQ68_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEZ0715202.1"
                     /translation="MSTQTNFDLVLFGATGDLAMRKLLPCLYQAHVAGLLHPEGRILG
                     VSRSELDTEGFLAKVETSSKIHVKENFSDEAWASFVERLAYLKVDVTQPDDFAALGDL
                     VKARKETDNVVIYLSTAPKFFAQTCENLAKIGLNADNVRVVLEKPLGTDLASSQQINT
                     DVARYFKEGQIYRIDHYLGKESLQNLLALRFANVMFEPLWNNKYIESVQLTIAEQLGV
                     EERGEFYDITGALRDMVQNHLMQMLCMTAMEAPASLDADAVRDEKVKVIKSLKPLTVE
                     SVNENVVRGQYTAAKGMNGYLEEINVPQDSFTETYVAIKAEIENERWKGVPFYLRTGK
                     RMAGKVAEIVLNFKDLNSHIFEGSRTAPNRLVIELQPYESVRLYTQMKTPGAGNKVET
                     VPLATDLGKALEGRRAEAYERLLLDVINGKLALFNRRDELEAAWEYVMPILENWTNNT
                     TPPHGYGAHSWGPEAARELLARDGHKWHEEQ"
     gene            7390..9225
                     /gene="edd"
                     /locus_tag="WGQ68_001421"
     CDS             7390..9225
                     /gene="edd"
                     /locus_tag="WGQ68_001421"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HEZ0715203.1"
                     /translation="MNTTPIHSKLAEITGRIIERSRPTREKYLAKIRSAKQMGRLERN
                     QLGCSNLAHGYAAMPKSIKIEMLQETVPNLGIITAYNDMVSAHQPFKDFPDQIKDEAQ
                     KNGATAQVAGGTPAMCDGITQGYAGMELSLFSRDVIAMSTAIGLSHQMFDGSLFMGVC
                     DKIVPGLMIGALSFGHIPGIFVPAGPMSSGIGNKEKARTRQLFAEGKVGRNELLKSEM
                     GSYHSPGTCTFYGTANSNQMMMEMMGVHLPAAAFVHPYTDLREALTRYAAGHLARGIK
                     NGTIKPLGEMLTEKSFINALIGLMATGGSTNHTMHLVAMARAAGVILNWDDFDEISSI
                     IPLLIRVYPNGKADVNHFTAAGGLPFVIRELLNAGLLHDDVDTVVGHGMRHYTKEPFL
                     IDGKLEWREAPETSGNDDILRKADNPFSPDGGLRLMKGNIGRGVIKVSAVREGCRIIE
                     APAIVFNDQREVLAAFERGELERDFVCVVRYQGPRANGMPELHKLTPPLGILQDRGFK
                     VALLTDGRMSGASGKVPASIHMTPEALIGGNIAKIRTGDLIRFDSVSGELNVLINETE
                     WNAREVESIDLGANQQGCGRELFANFRSMTSSAETGAMSFGGEFA"
     gene            9422..10060
                     /locus_tag="WGQ68_001422"
     CDS             9422..10060
                     /locus_tag="WGQ68_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEZ0715204.1"
                     /translation="MSKLTPREILTAGAVVPVMAIDDLSTAVDLSYALVEGGIPTLEI
                     TLRTPVGLDAIRLIAKEVPNAIVGAGTVTNPEQLKAVEDAGAVFAISPGLHESLAKAG
                     HNSGIPLIPGVATPGEIQLALEHGIDTLKLFPAEVVGGKAMLKALYGPYADVRFCPTG
                     GISLATAPEYLALPNVLCVGGSWLTPKEAVKNKDWDTITRLAKEAAALKPKA"
     gene            10075..10371
                     /locus_tag="WGQ68_001423"
     CDS             10075..10371
                     /locus_tag="WGQ68_001423"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715205.1"
                     /translation="MPSEKPFPFQTAFCRLRAQSAYTAALIRHTAPKMPIRLPHNKNN
                     VQFIRQQPDSPYYAAEKMPSETHSDGIHLKKTTDSTPINPLPKPLSSLGFRRRV"
     gene            complement(10319..11360)
                     /locus_tag="WGQ68_001424"
                     /pseudo
     CDS             complement(10319..11360)
                     /locus_tag="WGQ68_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225132.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
     gene            11523..12563
                     /gene="mutY"
                     /locus_tag="WGQ68_001425"
     CDS             11523..12563
                     /gene="mutY"
                     /locus_tag="WGQ68_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEZ0715206.1"
                     /translation="MNTPIPFSERLIRWQKQHGRHHLPWQVKNPYCVWLSEIMLQQTQ
                     VATVLDYYPRFLEKFPTVQTLAAAPQDEVLSLWAGLGYYSRARNLHKAAQQVVGQFGG
                     TFPSERKDLETLCGVGRSTAAAICAFSFNRRETILDGNVKRVLCRVFARDGNPQDKKF
                     ENSLWTLAESLLPSENADMPAYTQGLMDLGATVCKRTKPLCHQCPMADICEAKKQNRT
                     AELPRKKTAAEVPTLPLYWLIIRNRNGAILLEKRPAKGIWGGLYCVPCFESLNGLSDF
                     AAKFSLTMADMDEQTALTHRLTHRLLMITPFEGQMPSESPSDGIWIKPTHLKDYGLPK
                     PLEIYLNGNRLE"
     gene            12980..13540
                     /locus_tag="WGQ68_001426"
     CDS             12980..13540
                     /locus_tag="WGQ68_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEZ0715207.1"
                     /translation="MNMKTLLALAVSAVCSVSVAQAHEHNTIPKGASIEVKVQQLDPV
                     NGNKDVGTVTITESNYGLVFTPDLQGLSEGLHGFHIHENPSCEPKEKEGKLTAGLGAG
                     GHWDPKGAKQHGYPWQDDAHLGDLPALTVLHDGTATNPVLAPRLKHLDDVRGHSIMIH
                     TGGDNHSDHPAPLGGGGPRMACGVIK"
BASE COUNT         3173 a         3683 c         3449 g         3297 t
ORIGIN      
        1 cggtttaaat ttaattcact ataaaaacga caaaaacgca aaagccgccg acattcccgc
       61 atccaagttt cagtcaatca gataaccttg gatttctttg gttttcgcat tgatttctct
      121 ggtacggcag tcggattgtg ccacgccgta ttcgtcgccg tcagcgcatt tggcattcag
      181 accgttttgc cagttgcggt actcgggcat gacggtttcg gcaatatcgg cgggcagtgc
      241 ctgaaccgcg ctgttcagag ccgctttggc agttctttct tgtccgcttc cctgaccatt
      301 tcgtcgataa gggctttttt acggttgtac agctcttcca agcgtgcttc ggtttccacc
      361 gttttgcatg ccagttcgct gacttttatg ccgtttggcg tttcaccttc ggctttcgca
      421 ttgttggcac agattttgtc cataccgctt ttccatgttt tctgcgaagc ttgcagttta
      481 ttctgtacag tttgcggcaa tcctttccag aactgctcga actcgctgcg cgacaacttg
      541 tagcgttctt cccattcgga tacgcgggcg gcttcctgct cccgacccaa tgcctcctgc
      601 gccgccgctt cttctgccgc ttcaagttct tcgttccttt gtttcgcttt gtccaacggc
      661 tctttaatca gtgccatagc tgcggaacat atatgtttaa atttatgcaa accatcatat
      721 cgggattgca cacgctctgc aagtttaccg acggttttcc tgttcgataa aaatgccgtt
      781 tgaaacggtc ggcgttcaga cggcattttt ccgcaggttt tatttgcggt tggtctgcag
      841 gtagaggttg atcaggcgtt cggtcgaact gtcgtgcttt tgcggcccgg tttcgccggt
      901 cagttcgccc aaaatggttt tagccagttg tttgccgagt tccacgcccc actggtcgaa
      961 gctgttgatg ccccaaatga tgccttgtac gaaggttttg tgttcgtaca tggcaatcag
     1021 gctgcccata ttgcgcgggt tgaccttgtc catgagaatg aggttggtcg ggcggttgcc
     1081 ggagaaggtt ttgtgcggga ccagctcttc gatgcgcgcc tcatccatac cctgcgcttt
     1141 gagttcggcg cggacttcgt cgggggtttt gccgcgcata aaggcttctg cttgggcgaa
     1201 gacgttggca agcaggattt cgtggtgtcc gggcaggttg ctgcgttttt caagcgaggc
     1261 aatcaggtcg atgggggtaa tgtgcgtgcc ttggtgcagc agttggaaga aggcgtgctg
     1321 gccgttaatg cccgtttcgc cccagataat cggcgaggtt tcgtgtccga ctgctttgcc
     1381 gtccaacgta acctgtttgc cgttgctttc catatcgagc tgctggatga atttgggcag
     1441 gcggtgcaaa tgttggtcgt aaggcgcgat gacgtggctg ccgccgccgt agtagttgat
     1501 ataccagatg ccgatgaggg cgagaatgac gggcaggttg cgctcgagcg gtgtgttgat
     1561 gaagtgttgg tccatcaggt gcgcgccgtt gagcatttcg atgaagtttt cttcgccgag
     1621 atacagcata atcggcaatc cgatggcgga ccacaggctg taccgaccgc cgacccaatc
     1681 ccaaaattca aacatattgg cggtgtcgat gccgaattcg gcgacggctt tttgattggt
     1741 ggaaacggcg gcgaagtgtt tggcaacggc ttcttcgtcg cccgcatgat tcaaaaacca
     1801 ttcgcgcgcg gtcagcgcgt tggtcagcgt ttcctgcgtg gtaaatgttt tggaggcgat
     1861 gatgaacaac gtggtttcgg ggtggacttt ggacaatacg tcgcgcagtt gcgagccgtc
     1921 cacgttggag acgaagtgca tattgaggcg cggatgaccg aaaggtttga gcgcggtaca
     1981 catcatcagc ggacccaaat ccgatccgcc gatgccgatg ttgacaacgt cggtaatgac
     2041 ttggttggta tagcccagcc agcttccgct gcggacttcg tgtgcaaatt cgcccatacg
     2101 ttgcaaaacg cggttgactt tgggcatcac atcttcaccg tcaaccacaa tcggcgaatt
     2161 ggtgcggttg cgaagggcga catgcaggac ggcgcggttt tcggtggtat tgattttttc
     2221 gccgtggaac atctgccgca tccgctccgg cacgcctgct tctcgggcaa gctcgaacaa
     2281 aagcgacatg gtttcgtcgt tgatgcggtt tttggagtag tccagcgtca gtccgccgac
     2341 ttgcagccag tagcgttccg cacgctgcgg gtcttgctcg aacatttcgc gcatatgcaa
     2401 tgttttgctg tcgtcaaagt ggttccacaa tttcgaccat gcgggtaagt cgtgaaagtg
     2461 tttcatctat atgctcctga atgaggtttt ttgttgtggg atgaaaaggc tgctggaaac
     2521 tgccgcaagc cgccgacgac cgttgttcgg catttcagac ggcatttgtg ggatgccgtc
     2581 tgaaggtcaa tctttgtcgt aatcgatgtg cttgttgtgt atgctttttt tgcttttctg
     2641 caattgcagg ctggcagcat cgcccaagcg cagggcaagt ccgatggcga gaatgtcgat
     2701 gacggcaagc tgcaagaggc gggaaaccat gggcgtgtag agttcggcat tttcctgtgt
     2761 ggcaacgctc aacacgcagt cggcaagttg cgccagaggc gaatcgttgc gggtcagtgc
     2821 gatgacagac gcgccgtttt ctttggcgat gctgaccgca tccaaaagtt cgatagacga
     2881 acccgtgttg gaaatggcaa ccaaaacatc ctgatcgctc aaaacagatg ccgccatcag
     2941 ctgcgtgtgc gtatcgacat aggcgacggt ggacatgccg aaacggaaaa atttatgctg
     3001 cgcgtcctgt gccacaatgc cggaattgcc gacaccgtaa aactcgacgc gacgggcgtg
     3061 catcagcgtg gcaatggcgt tttccagctc cgactctttc aggaagcggc gttcgcccaa
     3121 cagcgaggcg gcggcattgc ccaacacttt ctcgaccacg cttgccatat cgtcgtcggc
     3181 gttgagttct tcgtggacat agggcatacc ctcatgaccg atgctggcgg acaaggcgag
     3241 cttgaactcg ggcagccctt tataacccaa gctgcggcag aatcggatga cggtcggctg
     3301 gctgacggac gcacgttcgg caatttcggc aacggcggca tggacgaacc atttgggttc
     3361 cgccaatgca cattcggcga ctttgcgttc cgcaccggaa aggtttgcca gtgattcgct
     3421 gattttgctt aacataatga tatgcccttc gataatgcag ccccgctgca aggagccgct
     3481 gtggttaaac gtttctcaaa tggttgtcga gagccgcagc cgcaccggaa attccgggaa
     3541 actcgctcaa gacgacatac acgggaatcg cggcaagata tgcttcaaac ctgcccttgt
     3601 tctcgaaacg gctgcggaac ggggaagttt tgaaatattc caacacgcgg ggaataatgc
     3661 cgccgcacag gtacacgccg ccgcgcgcgc ccagcgtcag ggcgaggttg gaagcaaccg
     3721 tgccgagcat ggcgcagaag atgtccaaag tctgacggca caaaggcgac gcgccgctca
     3781 aagccttttc cgtgatttca gacggcatca gtttggcggg tttggctttc tgttttgcag
     3841 ccaaagcctc gtaaaccaag ctcaagcccg cgccgctcaa aaagcgttcg gcggaaacat
     3901 ggccgtattt gtttttggcg tactgccaaa tcagcacttc catatcgtca aacggcggga
     3961 aactggtatg cccgccctcg cccgccaaag ccacccagcc tgcgtggctg tgcaccaatc
     4021 cgctcacgcc caggccggta ccggggccga taacggcttt gggggcaaat tcgacaggct
     4081 tttgcccgcc tacctgcatc aggtctttgc ttgaagtctg cgttaccgcc aatgcctgcg
     4141 cggtaaagtc gttcaaaagg atgagggtgt ccagccccaa agtctgacgg gtggtttcga
     4201 tggaaaacgc ccaatggtgg ttggtcatct gcacccagtc gcccaaaatc gggttggcga
     4261 tggcaaatgc cgcgtgccgt acggctgttg caccgctttg attcagatag gcacgcaccg
     4321 catcggtaac cgtatcgtag tctttacacg gaagcacggc ggctttttca atgacgcgcg
     4381 gcgcggtttc cagcgcaaag cgtgcattcg tcccgccgat atcggcgacc agtcggggat
     4441 atccggcttg tttattcggc gtagaagaca tggcagttca ctccttgatg gttcaaaacg
     4501 aggttgatcg gatattcgcg gttttcgcct tgtgcggctt gttcgaacac ggcttttttc
     4561 tcttcgccct gtatcgccaa aaatacatgc cccgtatggg caatcgcatc caaggtcatg
     4621 ctgacgcgct cgtgcggcgc ggtaacgggc gtggtatgca ccagcgcgac acctgccgaa
     4681 ccgtcgattg ccgtctgaaa ctgcggagct ttcgggaaaa tcgaagccgt atgcccgtcg
     4741 tttcccatac ccaaaaccaa aacatcgggc tgcttgtaat gtttcagtgc ataatcgaca
     4801 acagcatcgg gatgtaattc ggtttcagtt tttccgtctt ccaccatagg aatccacatt
     4861 gccgcttccg ctttgttctt caacaggtat tcgcgcacca aaccggtatt gctgtcggcg
     4921 tggacggtcg gcacgatgcg ttcatctgcc aaggtgatgc cgacgttttt ccaatccaaa
     4981 tctttttgcg acagggcgtt gaaaaatgca atcggcgaac gtccgccgga aactgccaac
     5041 accgcaccgc ccttctcgtc cagtgcgccc tgcaaagcat ccgccactgc gtcagtcaaa
     5101 gactgcgccg cttctgccgc attttcgtat tcgtgccaaa caaacatatt tgtgtccttt
     5161 ttttatttca gacggcatat tccgttatgg aaacgggttg agcaatatgt cggccgaaca
     5221 gttgtttatg cttttgatac caaatatcgg gactgctttt tatagtggat taaatttaaa
     5281 ccagtacagc gttgcctcgc cttgccgtac tatttgtact gtctgcggct tcgtcgcctt
     5341 gtcctgattt ttgttaatcc actatactgc tttacttgcg ttcagacggc atttcaaacc
     5401 ccatgccgtc tgaacgcatt attgtattac tgctcttcgt gccacttgtg tccgtcgcgc
     5461 gccaacagtt cgcgcgcggc ttcaggcccc cacgagtgtg cgccgtagcc gtgcggcggc
     5521 gtggtgttat ttgtccagtt ttccaaaatc ggcatcacat attcccacgc ggcttcgagt
     5581 tcgtcgcggc ggttgaacaa agcgagtttg ccgttaatca cgtccagcag caggcgctcg
     5641 taagcttccg cgcggcggcc ttccaatgct ttgcccaaat cggttgccag cggcacggtt
     5701 tcgaccttat ttcctgcccc cggggttttc atctgcgtat agaggcgcac ggattcatat
     5761 ggttgcaact cgataacgag ccggttgggc gcggtgcggc tgccttcaaa aatatggctg
     5821 ttcaaatctt tgaagttcaa aacgatttcc gccactttgc ccgccatgcg cttgccggta
     5881 cgcaggtaga agggaacgcc cttccagcgt tcgttttcga tttcggcttt aatggcgacg
     5941 taggtttcgg taaagctgtc ttgcggaacg ttgatttctt caagatagcc gttcatgcct
     6001 ttggcggcgg tatattgtcc gcgcacgacg ttttcattga cagactcgac ggtcagcggc
     6061 ttcaatgact tgatgacttt gactttttca tcgcgcaccg catcggcatc caagctggcg
     6121 ggggcttcca tcgcagtcat gcacagcatc tgcatcaaat ggttttgcac catatcgcgc
     6181 aacgcgccgg taatgtcgta aaactcgccg cgctcttcca caccgagctg ttcggcgatg
     6241 gtcaactgca cgctttcgat gtatttattg ttccacagcg gctcgaacat tacattggca
     6301 aaacgcagcg caagcaggtt ttgcaggctt tctttgccaa ggtagtggtc gatgcggtaa
     6361 atttgccctt ctttgaaata acgcgcaaca tcggtattga tttgctggga agaagccaaa
     6421 tccgtaccca acggtttttc caaaaccaca cgcacgttgt cggcgttcaa accgattttg
     6481 gcaaggtttt cacatgtttg cgcgaagaat ttcggcgcgg tggaaagata gatgacgaca
     6541 ttgtcggttt ctttgcgcgc tttgaccaaa tcgcccaaag cggcaaaatc gtccggctgc
     6601 gtaacatcga ctttgagata tgcgagacgt tcgacaaacg atgcccaagc ctcatcggaa
     6661 aaattttctt tcacatggat tttggaactg gtttccacct tcgccagaaa accttcggta
     6721 tccaactcgc tgcggctgac ccccaaaata cgcccttcgg gatgaagcag accggcaaca
     6781 tgcgcctggt acagacaggg caacagcttg cgcatcgcca aatcgccggt cgcaccgaac
     6841 aacaccaaat caaaatttgt ttgtgtactc atcgtattat ctcgtcagga aagaattttt
     6901 cgatgccgtt tgaaacctgt tttccccatc acgctgcatc gcaatatcgg aaacaaaggc
     6961 aggcggcata atgagtagta atactacaca ccgctacact ttttgtctat tcccattttt
     7021 acaatttatt tgacctagtc caaaaatcgg gcaggtttcc cctattccgt tacaacaatc
     7081 gaaagattct gcgatttaaa tcaaatttct tttcgatgtc tgattttttt gtaacaaaat
     7141 tacaaatttt gtactataat aacacccgct tcccactttc agacggcata ccttttaaaa
     7201 tatagtggat taacaaaaat caggacaagg cgacgaagcc gcagacagta cagataatac
     7261 ggcaaggcga ggcaacgccg tactggtttt tgttaatcca ctatacttac cgtctgaata
     7321 cccgatacaa aaatcagaaa cgcacaaaca aatccccaat accccccccg ttccgacagg
     7381 agaccgaccg tgaacactac tcctatccac tccaaactcg ccgaaatcac cgggcgcatt
     7441 attgaacgca gccgtccgac gcgtgaaaaa tatctggcga agatccgcag tgccaaacaa
     7501 atgggacgct tagagcgcaa ccagctcggc tgcagcaact tggcacacgg ctatgctgcc
     7561 atgcctaaaa gtatcaaaat cgaaatgctt caggaaaccg tccccaactt aggcatcatc
     7621 accgcctaca acgacatggt ttccgcacac cagccgttta aagacttccc tgaccaaatc
     7681 aaagacgaag cgcagaaaaa cggcgcgacc gcccaagtcg ccggcggcac gcccgccatg
     7741 tgcgacggca tcacgcaagg ctacgccggc atggaattgt cgctgttctc ccgcgacgtg
     7801 attgccatga gtaccgccat cgggctgtcg catcaaatgt ttgacggcag cctgtttatg
     7861 ggcgtatgcg acaaaatcgt tccaggtttg atgataggcg cgctttcgtt cggtcatatt
     7921 ccgggtatct tcgtccccgc aggcccgatg tccagcggta tcggcaacaa agaaaaagcc
     7981 cgcacccgcc agcttttcgc cgaaggcaag gtcggacgca acgaactttt gaaaagcgaa
     8041 atgggttctt accacagccc gggcacctgc actttctacg gcacggcaaa ctccaaccaa
     8101 atgatgatgg aaatgatggg cgtgcacctg cctgccgccg ccttcgtcca cccttacacc
     8161 gacctgcgcg aagcgctgac ccgctacgcc gccggacacc tcgcgcgcgg catcaaaaac
     8221 ggcacgatta aacctttggg cgaaatgttg accgaaaaat cctttatcaa cgccttgatt
     8281 ggcctgatgg caaccggcgg ttcgaccaac cacaccatgc acctcgtcgc tatggcgcgt
     8341 gcggccggcg tgattttgaa ctgggacgac ttcgacgaaa tttcctccat catcccgctg
     8401 ctcatccgcg tttatccgaa cggcaaggcc gacgtgaatc actttaccgc agcgggcggg
     8461 ctgcctttcg ttatccgcga attgctgaat gcaggcctgt tgcacgacga tgtcgatacc
     8521 gtcgtcggac acggtatgcg ccactacacc aaagagcctt tcctcatcga cggcaaactc
     8581 gaatggcgcg aagcccccga aaccagcggc aacgacgaca tcctgcgcaa agctgacaac
     8641 ccgttctccc ccgacggcgg tctgcgcctg atgaaaggca acatcggacg cggcgtgatt
     8701 aaagtgtccg ccgtgcgcga aggttgccgc atcattgaag cgcctgccat cgtgttcaac
     8761 gaccaacgcg aagtgttggc tgcgtttgaa cgcggcgagt tggaacgcga ttttgtgtgc
     8821 gtcgtccgct accaaggccc gcgtgccaac ggtatgcccg aattgcacaa actgaccccg
     8881 cctttgggca tcctgcaaga ccgcggcttc aaagtggcgc tgctgaccga cggccgtatg
     8941 tccggcgcgt ccggcaaagt tccggcctcc atccacatga cgcctgaagc cttgattggt
     9001 ggcaacatcg ccaaaatccg taccggcgac ctgatccgct tcgactccgt tagcggcgaa
     9061 ctcaacgtcc tgattaacga aaccgaatgg aatgcccgcg aagtcgaaag catcgacttg
     9121 ggcgcgaacc aacaaggctg cggccgcgaa ctcttcgcca acttccgcag catgaccagc
     9181 agcgcagaaa ccggtgccat gagtttcggc ggcgaatttg cctgatgcgc gtttcagacg
     9241 gccttttcag accgaaggcc gtctgaaaaa ttattgaagc tgtttaaacg gatcagacgc
     9301 agcgtgggct ttgcccgcga ataagcaact tccccccatt tttcaaaaag gccgtctgaa
     9361 aacctagccc cttcctgttt gacctcaaca aaaatgcccc tatcccactt ggagaactga
     9421 aatgtccaaa ctgaccccac gcgaaatcct gaccgccggc gcagttgtgc ctgtgatggc
     9481 gattgacgac ttaagcaccg ccgtcgattt gtcctacgcc cttgtcgaag gcggcatccc
     9541 taccctcgaa attaccctgc gtacacccgt cggcctcgac gccatccgcc tgattgccaa
     9601 agaagtgccc aacgccatcg tcggcgcggg tacggtaacc aatcccgaac agctcaaagc
     9661 cgtcgaagac gcaggcgcgg ttttcgccat cagcccgggg ctgcatgaat ccctcgccaa
     9721 agccggccac aacagcggca tccccctgat tcccggtgtt gccaccccgg gcgaaatcca
     9781 actggctttg gaacacggca tcgacaccct caaactcttc cccgccgaag tcgtcggcgg
     9841 caaagccatg ctcaaagccc tgtacggccc ttacgccgat gttcgcttct gcccgacagg
     9901 cggcatcagc ctcgccaccg cgcccgagta cttggcactg cccaacgtcc tgtgcgtcgg
     9961 cggctcttgg ctgacaccga aagaagccgt gaaaaacaaa gactgggaca ccatcacccg
    10021 cctcgccaaa gaagcggcgg cgttgaaacc caaagcctga ttcgcatcgt aaaaatgccg
    10081 tctgaaaaac ctttcccgtt tcagacggca ttttgccgat tgagggcaca gtcggcatac
    10141 acggcagcac tgatcagaca taccgcccct aaaatgccca tccgccttcc gcataataaa
    10201 aataacgttc agttcattcg acagcagccg gacagcccat actacgcggc tgaaaaaatg
    10261 ccgtctgaaa cgcattcaga cggcatccac ttaaaaaaaa caactgattc aacgccgatt
    10321 aatccgcttc caaaaccact ttcatcactt ggttttcggc ggcgtgtttg aacacgtcgt
    10381 aggctttttc caattcactg aatttgaaat gatgggtcag cattttggtg taatcgacgg
    10441 agctgctgga aatcgccttc atcagcattt cggtggtatt ggcgtttacc agaccggtag
    10501 taatggcgag gtttttaatc cagagttttt ccagtttgaa atcaacggat tgaccatgta
    10561 caccaaccac agcgatatgg ccgccgggtt tcacaatgtc ttggcacata ttccatgtag
    10621 cagggatacc gacggcttcg atggcgcaat ccacgccgtc ttcgccgacg atggcaaaga
    10681 cttgtttgga tacttcgccg gaagcagggt taatggtatg ggtcgcaccc aattctttcg
    10741 ccagtttcaa acggttttcg tccatatcgc aaacgatgat ggcggcggga ctgtacagtt
    10801 gggcggtcaa cagggcggac ataccgacag ggcctgcccc agcgatgaat acggtgtcgc
    10861 cgggtttgac atcgccgtat tgcacgccga tttcgtgggc ggtcggcaaa gcgtcgctca
    10921 acaacagggc gatttcttcg ttgacattat cgggcagcgg aacgaggctg ttgtcggcat
    10981 aaggcgtacg gacgtattcg gcctgagtac cgtcaatcat gtaacccaaa atccaaccgc
    11041 cgttacggca gtgtgaatag agttgggttt tgcagttgtc gcaagtgcaa catttgctga
    11101 cgcatgaaat aatgacttta tcgccgactt tgatgttttt tacagcctcg ccgacttctt
    11161 ctacaatacc gatgccctca tgaccgagaa tacggccgtc ggcgacttcg gttttttttg
    11221 cctttccaaa tacccaaatc ggtaccgcaa atcgtggttt tgacgatttt caccaccgca
    11281 tcggtcggat cgataatctg cggacggggt ttttcttcaa aacggatgtc gtttgcgccg
    11341 tgataaacca ttgctttcat gctgatactc cttgcttgtt gataaataat ttcaataccg
    11401 caataaagtt tccttatatg agttatatgc ccctacaaaa aataagtcaa taagaattat
    11461 tttcacaatg ttatacaata acatactatt ttaaatataa ataaaaccac cgattgatat
    11521 taatgaacac acccatcccc ttctccgaac ggctcatccg ctggcaaaaa caacacggtc
    11581 gccaccacct cccttggcag gtcaaaaacc cttattgcgt ctggctttcc gaaatcatgc
    11641 tccagcaaac gcaggtcgcc accgtgttgg actactatcc gcgtttctta gaaaaattcc
    11701 cgactgttca gacgcttgcc gccgcgccgc aagacgaagt gttgtcgttg tgggcgggct
    11761 tgggctatta cagccgcgcg cgcaatctgc acaaagccgc gcaacaagtc gtcggacaat
    11821 tcggcggcac atttccatcg gagcgtaaag acttggaaac cctctgcggc gtaggcagaa
    11881 gcaccgccgc cgccatttgc gccttctcct tcaaccgccg cgaaaccatt ttggacggca
    11941 acgtcaaacg cgtactctgc cgcgtgttcg cccgcgacgg caatccgcag gacaaaaaat
    12001 ttgaaaactc gctctggaca cttgccgaaa gcctgctgcc gtctgaaaac gccgatatgc
    12061 ctgcctatac acaaggtttg atggatttgg gcgcgaccgt gtgcaaacgg acgaaaccct
    12121 tgtgccacca atgcccgatg gcggacatct gcgaagcgaa aaagcaaaac cgcaccgccg
    12181 agctgccgcg caaaaaaacc gccgccgaag taccgaccct gccgctttac tggctgatta
    12241 tccgcaaccg gaacggcgcg attttgctgg aaaaacgccc cgccaaaggc atttggggcg
    12301 ggctgtattg cgtgccgtgt tttgaaagtt tgaacgggct ttccgacttt gccgccaaat
    12361 tctccctgac catggcagat atggacgaac aaaccgccct gacccaccgg ctgacgcacc
    12421 ggttgctgat gattacgccg tttgaaggac aaatgccgtc tgaaagccct tcagacggca
    12481 tttggataaa gccgacgcat ttgaaagatt acggtttgcc caagcctttg gaaatttatt
    12541 taaacggtaa taggttagaa taaacaaaat aaacccattg aactgttgtt tgcaggtatc
    12601 gcagcaagaa caaccgatga atttgggtcg tattttaggc ggcgggataa tgttcaaatg
    12661 ggacatttgg aacggaagaa gtcggcaatt taaaaaggat ttaaaaagca aagaaggtca
    12721 aaaacatgaa cacaaactta aatgacaaag acaaagccat ggataccgca atcaggtttc
    12781 agaaaaggat gaggattccg aaatttttct ttttaattct cggaatcaca atggttttgg
    12841 catttatcca agacgtgata acgggttcta attttctgca aataacaatt aatgtaaaat
    12901 tttcgtaaaa atttatcggc ttttaaaaca aaattgacta aaatagtcgc gagtttttac
    12961 tgcaataaag gagattgcaa tgaatatgaa aaccttatta gcactagcgg ttagtgcagt
    13021 atgttcagtt agtgttgcgc aagcacacga gcataatacg atacctaaag gtgcttctat
    13081 tgaagtgaaa gtgcaacaac ttgatccagt aaacggaaac aaagatgtgg gtacagtgac
    13141 tattactgaa tctaactatg gtcttgtgtt tacccctgat ttacaaggat taagcgaagg
    13201 cttacatggt ttccacatcc atgaaaaccc aagctgtgag ccaaaagaaa aagaaggtaa
    13261 attgacagct ggtttaggcg caggcggtca ctgggatcct aaaggtgcaa aacaacatgg
    13321 ttacccatgg caagatgatg cacacttagg tgatttacct gcattaactg tattgcatga
    13381 tggcacagca acaaatcctg ttttagcacc acgtcttaaa catttagatg atgttcgcgg
    13441 tcactctatt atgatccaca cgggtggtga taatcactcc gatcatccag ctccacttgg
    13501 cggtggcggc ccacgtatgg catgtggcgt gattaaataa ttcgattgtt cgaaacgaaa
    13561 agtgcggtaa attttgaccg cacttttttg ctagatattt ag
//
LOCUS       DAWFHY010000057        13573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.057, whole genome shotgun sequence.
ACCESSION   DAWFHY010000057 DAWFHY010000000
VERSION     DAWFHY010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 13573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.057"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..270)
                     /locus_tag="WGQ68_001427"
     CDS             complement(<1..270)
                     /locus_tag="WGQ68_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006249760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0715208.1"
                     /translation="MRKSRLSRYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGKVEADESYFAERQNHINGIGNFWNRAKRH"
     gene            complement(427..3177)
                     /gene="gyrA"
                     /locus_tag="WGQ68_001428"
     CDS             complement(427..3177)
                     /gene="gyrA"
                     /locus_tag="WGQ68_001428"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEZ0715209.1"
                     /translation="MTDATIRHDHKFALETLPVSLEDEMRKSYLDYAMSVIVGRALPD
                     VRDGLKPVHRRVLYAMHELKNNWNAAYKKSARIVGDVIGKYHPHGDIAVYDTIVRMAQ
                     NFAMRYVLIDGQGNFGSVDGLAAAAMRYTEIRMAKISHEMLADIEEETVNFGPNYDGS
                     EHEPLVLPTRFPALLVNGSSGIAVGMATNIPPHNLSDTVNACLRLLDAPDTEIDELID
                     IIQAPDFPTGATIYGLSGVREGYKTGRGRVVIRAKTHTEPIGKNGEREAIVIDEIPYQ
                     VNKAKLVEKIGELVREKTLEGISELRDESDKSGMRVVIELKRNENAEVVLNQLYKLTP
                     LQDSFGINMVVLVDGQPRLLNLKQILSEFLRHRREVVTRRTLFRLKKARHEGHIAEGK
                     AVALSNIDEIIKLIKESPNAVEAKEKLLARPWRSSLVEEMLTRSGLDLEMMRPEGLAA
                     NIGLKEQGYYLSEIQADAILRMSLRNLTGLDQEEIVESYKNLMGKIIDFVDILSKPER
                     ITQIIRDELEEIKTNYGDERRSEINPFGGDIADEDLIPQREMVVTLTHGGYIKTQPTT
                     DYQAQRRGGRGKQAAATKDEDFIETLFVANTHDYLMCFTNLGKCHWIKVYKLPEGGRN
                     SRGRPINNVIQLEEGEKVSAILAVREFPEDQYVFFATAQGLVKKVQLSAFKNVRAQGI
                     KAIALKEGDYLVGAAQTGGADDIMLFSNLGKAIRFNEYWEKSGNDEAEDADIETEISD
                     GIEDETADSENALPSGKHGVRPSGRGSGGLRGMRLPADGKIVSLITFAPETEESGLQV
                     LTATANGYGKRTPIADYSRKNKGGQGNIAINTGERNGDLVAATLVGETDDLMLITSGG
                     VLIRTKVEQIRETGRAAAGVKLINLDEGETLVSLERVAEDESELSDASVISNVTEPEV
                     EN"
     gene            complement(3275..3775)
                     /gene="hscB"
                     /locus_tag="WGQ68_001429"
     CDS             complement(3275..3775)
                     /gene="hscB"
                     /locus_tag="WGQ68_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly co-chaperone HscB"
                     /protein_id="HEZ0715210.1"
                     /translation="MSQYFTLFRIEPAFDIDTENLEQTYRALAARFHPDKFASASAFE
                     QKQAVMMSSTINDAYRTLKNPIDRAAYLLKTSGIDADAPEHTAFAPEFLMQQMEWRET
                     LMEARAGNDLESLKNLDNEIRDEQEKLFCGLKQSFARQDYDTAAQQVRQGRFLDKLRN
                     EISSAL"
     gene            3857..4078
                     /locus_tag="WGQ68_001430"
     CDS             3857..4078
                     /locus_tag="WGQ68_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor A"
                     /protein_id="HEZ0715211.1"
                     /translation="MGGKVQHNKGKIRDNALKALVKSDLFRHKVERKRKGKGSYNRQE
                     AKKWRDGFDTVPPFLCLKHGSFRRSRRSL"
     gene            complement(4038..4358)
                     /gene="iscA"
                     /locus_tag="WGQ68_001431"
     CDS             complement(4038..4358)
                     /gene="iscA"
                     /locus_tag="WGQ68_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HEZ0715212.1"
                     /translation="MITLTENAAKHINDYLAKRGKGLGVRLGVKTSGCSGMAYNLEFV
                     DEADGDDLIFEGHGARIYIDPKSLVYLDGTQVDYTKEGLQEGFKFENPNVKDSCGCGE
                     SFHV"
     gene            complement(4445..4831)
                     /gene="iscU"
                     /locus_tag="WGQ68_001432"
     CDS             complement(4445..4831)
                     /gene="iscU"
                     /locus_tag="WGQ68_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HEZ0715213.1"
                     /translation="MAYSDKVIDHYENPRNVGTFDKNDESVGTGMVGAPACGDVMRLQ
                     IKVNDEGIIEDAKFKTYGCGSAIASSSLITEWVKGKSLDDALAIKNSEIAEELELPPV
                     KIHCSILAEDAVKAAVADYRKRQENR"
     gene            complement(5213..5917)
                     /locus_tag="WGQ68_001433"
     CDS             complement(5213..5917)
                     /locus_tag="WGQ68_001433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715214.1"
                     /translation="MSNKTCFVVMAIGNQHHNGQTITAEDLKARYDNLIKEAILKARP
                     SIEVTRADEVSAMGTITTDIVQRIMYSDYIIADVSYPNPNVFYELGLRHACKPGTIII
                     KDKDAKVPFDIASLRYIEYENSTQGLKDLSNKLLQTFDNFDRDNNRPDNHFLEVAKLT
                     SFKFPEFFKEDPLEVQFMMSLLQSPELIDLFSRQQRGEEVDSSEIMQSLSTHPNILKP
                     VLKYLLQSGELNLLPK"
     gene            complement(6009..7223)
                     /locus_tag="WGQ68_001434"
     CDS             complement(6009..7223)
                     /locus_tag="WGQ68_001434"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HEZ0715215.1"
                     /translation="MTVKTPVYLDYAATTPVDKRVAEKMIPYLTETFGNPASNSHAFG
                     WEAEEAVEKARADIAALINADPKEIVFTSGATESDNLAIKGAANFYKTKGKHLITVKT
                     EHKAVLDTMRELERQGFEVTYLGVQENGLIDLEELKAAIRDDTILISVMWVNNEIGVV
                     QDIPAIGEICRERKIVFHVDAAQACGKVPVDVEAAKIDLLSMSAHKVYGPKGIGALYV
                     RRKPRVRLEAQMHGGGHERGFRSGTLPTHQIVGMGEAFRIAKEELEQDMAHYRKLRDI
                     FLKGIEGIEEVYVNGDLEHRAPNNLNVSFNFVEGESLIMAVKELAVSSGSACTSASLE
                     PSYVLRALGRNDELAHSSLRITFGRMTTEEEVQFAAELIKSKIGKLRELSPLWEMFKD
                     GIDLNSIEWAAH"
     gene            complement(7252..7698)
                     /gene="iscR"
                     /locus_tag="WGQ68_001435"
     CDS             complement(7252..7698)
                     /gene="iscR"
                     /locus_tag="WGQ68_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004519894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HEZ0715216.1"
                     /translation="MRLTTKGRFAVTAMLDLAMNAQTGAVKLSAISERQNISLSYLEQ
                     LFGKLRRAGLVESLRGPGGGYILAAPAARINIAQIIAAAEDRLDATQCGSKANCHHGA
                     PCLTHDLWENLNKTINDYLGSVTLQSIIEQKNNGDGSRVVQFTHIH"
     gene            8047..9219
                     /locus_tag="WGQ68_001436"
     CDS             8047..9219
                     /locus_tag="WGQ68_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEZ0715217.1"
                     /translation="MKRDLSKMTCIEDLRRVAKRKMPRMFYDYIDSGSWTETTYRENT
                     SDFKDIRFRQKVLVNMEGRSLETKMIGQDVKMPVAIAPTGFTGMAHADGEILAARAAE
                     KFGIPFTLSTMSICSIEDVAENTSAPFWFQLYVMRDREFMENLIKRAKDAKCSALVLT
                     ADLQVLGQRHKDIKNGLSAPPKPTIANLINLATKPEWCMKMLNTERRTFRNIVGHAKN
                     VGDLSSLSSWTSEQFDPRLSWDDVARIKDLWGGKLIIKGIMEPEDAEKAAKSGADALV
                     VSNHGGRQLDDTVSAIKALPDIVSAVGSDIEVWMDSGIRSGQDILKAWALGAKGTMIG
                     RAFLYGLGAYGEEGVTRALEILYKEMDISMAFTGHRDIQDVDASILRGKDWGRETV"
     gene            complement(9355..12132)
                     /locus_tag="WGQ68_001437"
     CDS             complement(9355..12132)
                     /locus_tag="WGQ68_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEZ0715218.1"
                     /translation="MANNKTLFEVIENERKAVKKYKPELLEMPEFTSKNLKYDFFEWQ
                     KSALENFLIFDRTSKLDDFPDLKNKPTHLLFNMATGAGKTMMMAALILYYFEKGYRHF
                     LFFVNQNNIVDKTENNFIDPTHAKFLFTEKILQGDTVIPIRKVETFSPHSDGIEIKFT
                     SIQKLYNDIHTERENQTTLADLHELNLVMLGDEAHHLNAQTKGKKQGELDLEVELKAN
                     AGNAEIERKGWEHMVLELLLNKNGNPSQNVLLEFTATLPENADVQQKYADKIITKFGL
                     KEFLQKGYTKEINLVSSTLGKKERVLHALLFAWYRHQIALKYGIANFKPVMLFRSKTI
                     DESKADYLAFLNWAENVQAVDFSFLTTFSTSLNDSDNANEQGKTRTEQALKFMQEKGV
                     EFAHLADWVKQNYQKHNVIITNSETNKTKTEKTDSETEKLLNNLEAADNPIRAIFTVD
                     RLTEGWDVLNLFDIVRLYEGQNGGGSNKKSGKTAAATVSEKQLIGRGVRYFPFAFEGK
                     QPNKRKFDNDMQHELRILEELFYYTHDEQSRYISELKNELRKDGYLPEKDDDKVLATF
                     KLKSEFAANKDFRELLIWANKKIPNPNAKTNNANSLQANPQTLSFQVHGNQLLQETQF
                     TADENDEKVKQLGTQNNFTQTIKMSEMERHIFNKALHIKGKNSQSLFHFDRLQSKLNI
                     QNRNELQNKLLKDWQIEFLGLGQDKQVRLDDKLAGCLKILEMVEKHLNESDMPFIGTK
                     EFTPKKLWEIFGTPKQKWVKKDDVKTTIATQNDWYVMNNFAGTGLEEALIQFISARLG
                     DLKSQYDVHLIRNEEVFKLNNFADGEGFMPDFILLLKDKQKSSSNGVNDFLHYQIFIE
                     PKGEHLVETDGWKEEFLEAITVEYGMDKILQKDTPHYRLIGLPFFTDHQKNGQFTESF
                     PLGAASLEKMK"
     gene            complement(12122..13315)
                     /locus_tag="WGQ68_001438"
     CDS             complement(12122..13315)
                     /locus_tag="WGQ68_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEZ0715219.1"
                     /translation="MDPPYNTETDSFAYNDKFNRSTWLTFMKNRLEIARELLADDGAI
                     YINLDYHEVHYCKVLMDEIFGEYNFQREIIWRIGWVSGYKTTVSNYIRNHDTILFYSK
                     DYNHLNFNKYYIDNKHFKVLVDLDRATKNTFTKFGINKETAEKILQEINFSNRPERYP
                     LEDVWNANEYDDLNSIAIVSYSGESVSKMLGLEEIKGQKSEKLLKRILEAHTKPNDIV
                     LDFFGGSGTTAAVAHKMNRQYICIEQIDKQTDTIVKRLNKILVNESSGISKAVNWQGG
                     GEFVYAELAPFNETAKQQILACEDSDGIKTLFEGLCERYFLKYNVSINEFSQIIEEPE
                     FQSLPLDEQKQMVLEMLDLNQMYVSLSEMDDEQFADCLNDDDKALSRAFYQSVKNQAE
                     KKDGE"
BASE COUNT         3130 a         2953 c         3342 g         4148 t
ORIGIN      
        1 atgacgtttt gcccggttcc aaaagttccc aattccattg atatggtttt gtcgttcagc
       61 aaaataactt tcatctgctt ctactttgcc gtcaaacatt tccaaatgcg gactgttttg
      121 ataaataagt aatcgtaaac gatgaaaata ataggctgcg gtatttttat taacgcctac
      181 taactctgct gccgttcttg cagttacacc tgcgacaaac agttcaatga gtttattttg
      241 tttataccgg cttagacgac tttttctcat aggggcaact ctaacttaat ttgaatttcc
      301 ctagttatct aggacagccc cttgttttta attgactata atccgctata ttgtgagaaa
      361 ctggatgacg gatgagggat aaaataaatg ccgtctgaat cttcagacgg catcgggaga
      421 tgattttcag ttctcgactt ccggttcggt tacattggaa attacagaag cgtcggagag
      481 ttcggattcg tcttcggcaa cacgttccag cgataccaag gtttcgcctt cgtccaagtt
      541 aatcagtttc acgcctgctg cggcgcggcc ggtttcgcgg atttgttcga ctttggtgcg
      601 gataagtacg ccgccgctgg taatcagcat caaatcgtcg gtttcgccga ccaaggttgc
      661 ggcgaccaaa tcgccgtttc gctcgccggt gttaatggca atattgcctt gcccgccttt
      721 gtttttgcgg ctgtaatcgg caatcggggt gcgttttccg tatccgttgg cggtggcggt
      781 taaaacttgc aaaccgcttt cttcggtttc aggggcgaag gtaatcaggc tgacgatttt
      841 gccgtcggca ggcaggcgca taccgcgcaa accgccgctg ccgcgaccgg acgggcgaac
      901 accgtgtttg ccgctcggca gtgcgttttc gctgtcggcg gtttcatctt cgatgccgtc
      961 tgaaatttcg gtttcgatgt cggcatcttc cgcttcgtcg ttgccggatt tttcccagta
     1021 ttcgttgaag cggatggctt tacctaagtt ggagaacagc atgatgtcgt ccgcaccgcc
     1081 tgtttgcgca gcgccgacga ggtagtcacc ttctttgagc gcgatggctt taatgccttg
     1141 ggcgcggacg tttttaaagg cggaaagttg gacttttttc accagtccct gcgcggtggc
     1201 gaagaagacg tattggtctt cggggaactc gcgaactgcc aaaatcgcgc tgactttttc
     1261 gccttcttcc aactggatga cgttgttaat cggacgaccg cggctgttgc ggccgccttc
     1321 gggcagcttg taaaccttaa tccagtggca cttgccgagg ttggtaaaac acatcagata
     1381 gtcgtgcgtg ttcgcgacga acagggtttc gatgaagtct tcgtctttgg tggctgcagc
     1441 ctgtttgcca cgcccgccgc gacgctgagc ctgatagtcg gtggtcggct gggtcttgat
     1501 atagccgccg tgggtcaggg tcacgaccat ttcgcgttgc ggaatcaggt cttcatcggc
     1561 aatgtcgccg ccgaacgggt tgatttcgct gcggcgttcg tcgccatagt tggttttgat
     1621 ttcttccagt tcgtcgcgga tgatttgggt aatgcgttcg ggtttggaga ggatgtccac
     1681 aaagtcgatg attttaccca tcaggttttt gtagctttcg acaatttctt cttgatcgag
     1741 gccggtcagg tttcgcaggc tcatgcgtaa aatagcatct gcctgaatct cgctcaggta
     1801 ataaccttgc tctttcaagc cgatgtttgc agccaatcct tccggacgca tcatttccaa
     1861 atccagaccg gaacgcgtca gcatttcttc aacgaggctg ctgcgccaag gacgagcaag
     1921 cagtttttct ttggcctcga ctgcgttggg agattctttg atgagcttga tgatttcatc
     1981 gatattggac agtgcgacgg ctttgccttc ggcaatatgc ccttcatggc gagccttctt
     2041 cagtcggaaa agcgtacgtc gggtaacgac ttcgcggcgg tggcgcagga attcggagag
     2101 aatctgcttc aggttcaaca ggcgcggttg tccgtcgacc aaaactacca tattgatgcc
     2161 gaaactgtct tgcagcggag tcagtttgta gagttggttt aagacgactt cggcattttc
     2221 gttgcgtttc agctcgataa cgacgcgcat cccggatttg tcggattcgt cgcggagctc
     2281 ggaaatgcct tccagcgttt tttctcggac cagttcgccg attttctcga ccagcttggc
     2341 tttgttgacc tgatagggga tttcgtcgat aacgatggct tcgcgttcgc cgtttttacc
     2401 gatgggttcg gtgtgggtct tggcacggat gacgacgcgg ccgcggccgg ttttatagcc
     2461 ttcgcgcacc ccgctcaagc cgtagatggt tgccccggtc gggaagtcgg gggcttggat
     2521 gatgtcgatc aattcgtcga tttcggtgtc gggtgcatcg agcaggcgca ggcaggcgtt
     2581 gacggtgtcg gacaggttgt gtggcgggat attggtcgcc ataccgacgg caatgccgga
     2641 cgagccgttg acgagcaggg cggggaagcg ggtgggcagt acaagcggct cgtgttcgct
     2701 gccgtcgtag ttcgggccga aattgacggt ttcttcctca atatccgcca gcatttcgtg
     2761 ggaaattttc gccatacgga tttcggtgta acgcatcgcg gcggcggcaa gcccgtcaac
     2821 cgatccgaag ttgccctgtc cgtctatcag cacatagcgc atagcgaaat tttgcgccat
     2881 acggacgatg gtgtcgtata cggcgatatc gccgtggggg tggtatttac cgatgacgtc
     2941 gccgacgatg cgcgccgatt ttttgtaggc ggcattccag ttgtttttca attcgtgcat
     3001 cgcgtacagt acgcggcggt gtaccggctt gagaccgtcg cgaacgtccg gcagcgcacg
     3061 cccgacaatg acgctcatgg cgtagtcgag atagcttttg cgcatttcgt cttcaaggct
     3121 taccggcagg gtttcgaggg cgaatttgtg gtcgtggcgg atggttgcgt cggtcatggt
     3181 ttcaatgttt cgtatggcaa aaaattgttg cttattttag catattttga cgcggaacgg
     3241 tgcggcggtt acgccgtctg aaacacggtg cggattataa tgccgaggaa atttcgttgc
     3301 ggagtttgtc gagaaacctg ccttggcgga cttgttgtgc ggctgtgtcg taatcttgtc
     3361 gggcaaacga ctgtttcaga ccgcagaaca gtttttcttg ttcgtcgcgg atttcgttgt
     3421 cgagattttt caaggattca aggtcgttgc ctgcccgtgc ctccatcagc gtttcgcgcc
     3481 attccatttg ctgcataagg aattcggggg cgaaagcggt atgctccggc gcgtcggcat
     3541 cgatgcccga tgttttcagc aggtaggcgg cgcggtcgat ggggtttttc aaggtgcggt
     3601 aggcatcgtt gatggtggaa gacatcatca ctgcctgctt ttgctcaaag gcggaagctg
     3661 aagcgaattt atcgggatgg aaacgggcgg ccaaggcgcg gtaggtttgt tccaagtttt
     3721 cggtgtcgat atcgaaagcg ggttcaatcc ggaagagggt gaaatattgg gacatagtag
     3781 gatgataaat gtaagatttt ggcagaaaac tgtttttgcc ttataatctg cagcttctta
     3841 aacgaaagga ctgaatatgg gcggcaaagt gcagcacaat aaaggcaaaa tacgcgacaa
     3901 tgctttaaaa gccttagtga aatccgattt gttccggcac aaggtggaac ggaaaaggaa
     3961 aggcaaaggc agctacaaca ggcaggaagc gaaaaaatgg cgggacggtt ttgatactgt
     4021 cccgccattt ttatgtctta aacatggaag ctttcgccgc agccgcagga gtctttgaca
     4081 ttggggtttt caaatttgaa accttcctgc aaaccttctt tggtgtaatc gacttgtgtg
     4141 ccgtccagat aaaccaggct tttcgggtcg atataaatgc gtgcgccgtg tccttcaaaa
     4201 atcaggtcgt cgccatcggc ttcatcgaca aattcaaggt tgtacgccat ccccgagcag
     4261 ccgctggttt tcacacccaa gcgtacgccc aagcctttgc cgcgtttggc gagatagtca
     4321 ttgatgtgtt ttgcggcatt ctcggtaagg gtaatcatat ttcttccttg ttgtatcgcc
     4381 ccggacagac ctgaagcggc gggtggttcg gacggcattg cgggacgatg ccgtctgaag
     4441 ggctttatct gttttcctga cgtttgcggt agtcggcaac ggccgctttt accgcatctt
     4501 cagccaagat ggagcagtgg atttttaccg gcggcaattc caactcctcg gcgatttcgc
     4561 tgtttttgat tgccagcgcg tcatccaggc ttttgccttt aacccactcg gtaatcaggc
     4621 tggacgaagc gatggcggaa ccgcagccgt aagttttgaa tttcgcatct tcgatgatgc
     4681 cttcatcgtt cactttgatt tgcaggcgca tcacgtcgcc gcaggccggt gcgccgacca
     4741 tgccggtgcc gacagattcg tcgtttttgt cgaaagtacc gacgttgcgg ggattttcgt
     4801 agtggtcgat tactttatcg ctgtatgcca tgatgtggtt tccttaatgt tttgatgatt
     4861 taagtggttt gtttgccggt tttcagacgg cctgaattag attttgcacg cgccgccttc
     4921 gcagccgtca tcattctctg aatcgacgcc gccttccatg gtaacgccgt caaaatcttc
     4981 aagcaggttg tcgagattgt cgaggtcaag ttcttgttcg ttcatgggtt gcatccttaa
     5041 ttttaatgtg gtggctttta ggtcgtctga aaaggtcttt tgggatttta gacgaccttt
     5101 tttgttttta atgtgtggat tgaatggggt gggttgtcaa gcctaatgtt tcgggtggat
     5161 tgatggactt gttggatttt caacctagtc tacatttttt attttctata gtttatttag
     5221 gtaatagatt tagttcccca gattgcaaca aatattttaa aacaggtttt aaaatatttg
     5281 gatgagttga gagagattgc attatttcgc tagaatcaac ttcttctcca cgttgttgtc
     5341 ttgaaaataa atcaattagc tctggagact gaagtaatga catcatgaac tgtacctcca
     5401 atgggtcttc tttaaagaat tcaggaaatt taaaggaagt taattttgca acctctagga
     5461 aatggttgtc tggtctatta ttatctcgat caaagttatc gaaggtttgc aaaagtttgt
     5521 tagatagatc ttttaatcct tgtgttgaat tttcatattc aatatatctc aacgatgcaa
     5581 tatcaaaagg aacttttgca tctttgtcct taataatgat agtacctggt ttacacgcat
     5641 gccttaatcc aagttcataa aacacattag gattaggata actaacatcc gcaataatat
     5701 aatcagaata cattattctt tgtactatat cagttgtaat tgtccccatt gctgaaactt
     5761 catcagcacg tgtaacttcg atactaggac gggcttttaa gatagcttcc ttaatcaggt
     5821 tgtcatatct tgcctttaaa tcttcagctg taatggtttg tccgttatga tgttgatttc
     5881 ctattgccat aacaacaaaa catgtcttat tggacataat tcaactcccc tttttattta
     5941 ccagtaataa ataatatttc cactaaaatt tgatttaagc actcaaccta cacaataaag
     6001 tacattaatc aatgcgccgc ccattcaatt gagtttaaat caatcccgtc tttgaacatt
     6061 tcccacagcg gcgacagttc gcgcagtttg ccgattttgg atttaatcag ttcggcggcg
     6121 aattgcactt cttcttcggt ggtcatgcgg ccgaaagtga tgcgcaggga tgagtgcgcc
     6181 agttcgtcgt tgcgaccgag cgcgcgtagg acgtagcttg gttcgagact ggcagaagta
     6241 caggcggagc cgctggatac ggcgagttct ttcaccgcca taatcaggct ttcgccttcg
     6301 acgaagttga agctgacgtt caggttgttc ggggcgcggt gttcgaggtc gccgttgaca
     6361 tagacttctt cgatgccttc gatgcctttg aggaagatgt cgcgcagttt gcggtagtgc
     6421 gccatatctt gttcgagttc ttctttggcg atgcggaagg cttcgcccat accgacgatt
     6481 tggtgggtcg gcaatgtgcc gctgcggaag ccgcgttcgt ggccgccgcc gtgcatttgg
     6541 gcttcgaggc ggacgcgggg tttgcggcgg acgtacagcg cgccgatgcc tttggggccg
     6601 tacactttgt gcgcggacat cgagagcagg tcgattttgg cggcttcgac atcgacaggc
     6661 actttgccgc aggcttgggc ggcatcgacg tggaagacga ttttacgttc gcggcagatt
     6721 tcgccgatgg cgggaatgtc ttgcaccacg ccgatttcgt tgtttaccca cattacggaa
     6781 atcaggatgg tgtcgtcgcg gatggcggct ttgagttctt ccaaatcaat caaaccgttt
     6841 tcttgaacgc cgaggtaggt tacttcaaaa ccttggcgtt cgagttcgcg cattgtatct
     6901 aaaacggctt tatgttcggt tttgacggta atgaggtgtt tgcctttggt tttgtagaag
     6961 tttgccgcgc ctttgatggc gaggttgtcg gactcggtcg cgccgctggt gaagacgatt
     7021 tctttggggt cggcattaat cagggcagcg atgtcggcgc gggctttttc gacggcctct
     7081 tctgcttccc agccgaatgc gtggctgttg gaggcggggt tgccgaaggt ttcggtcaga
     7141 tagggaatca ttttttcggc aacgcgtttg tcaacggggg tagtggcggc gtagtcgagg
     7201 taaacggggg ttttgacggt catggtttgc tctttctttt tcgggtgtta tttaatggat
     7261 gtgtgtaaat tggacgacgc ggctgccgtc gccgttgttt ttctgttcga tgatgctttg
     7321 cagggtaacg ctgccgaggt agtcgttgat ggttttgttt aaattctccc aaaggtcgtg
     7381 cgtcaggcag ggcgcgccgt ggtggcagtt ggctttgctg ccgcattggg tcgcgtccag
     7441 ccggtcttcg gcggcggcga tgatttgggc gatgttgatg cgtgccgccg gtgcggcgag
     7501 gatgtagccg ccgccgggtc cgcgcagact ttcaaccagt ccggcgcggc ggagtttgcc
     7561 gaacaattgc tcaagatagg agagggatat gttttggcgt tcgctgatgg cactgagttt
     7621 gacggcgccg gtttgcgcgt tcatcgccaa atccagcata gcggtaacgg cgaaacgccc
     7681 tttggtggtc agtctcatgg tggtggttgc ccgtgtcggc tttttatagt gggcggaatt
     7741 gtccaatatc tgagtgtttc agtcaagtat gggggagctg ttgccgattg tttgtttaaa
     7801 tggcggtata gtctgatttt gctttatttg tttggttttt taaatcagtt ggttatgttt
     7861 tttgaggagc ggcggttggg gcgttttgcc ggcaagcggt ttcaaaacga tttgatttat
     7921 ttttgatatt gatatagatt atctaaatct tagtgattta tttgggtatt gcctgaaatg
     7981 ctgtctgaag gctcaagcgg tttcggacgg catattccat caggctgtca aacagggaaa
     8041 aacaagatga aacgtgattt gagcaaaatg acctgcatcg aagacttgcg ccgcgttgcc
     8101 aagcgcaaaa tgccgcgtat gttttacgat tacatcgatt cgggttcgtg gacggaaacg
     8161 acttatcggg aaaatacttc ggatttcaaa gacatccgtt tccgacaaaa agtgttggtc
     8221 aatatggagg ggcgcagcct cgaaaccaaa atgattgggc aggatgtgaa aatgccggtg
     8281 gcgattgccc cgactggttt cacgggtatg gcgcacgcag acggtgaaat cttggcggcg
     8341 cgggcggcgg agaagttcgg cattccgttt acgttgtcca ccatgtccat ctgctcgatt
     8401 gaagacgttg ccgagaacac ttccgcacca ttttggtttc agctttatgt gatgcgcgac
     8461 cgcgagttta tggaaaacct gattaagcgc gcgaaggatg ccaaatgttc ggcattggta
     8521 ttgaccgccg atttgcaggt tttgggtcaa cgccacaaag acatcaaaaa cggcctgtcc
     8581 gcgccgccga aaccgaccat cgccaattta atcaatctgg cgaccaaacc ggaatggtgc
     8641 atgaaaatgc tgaacacgga acgccgcacg ttccgcaata tcgtcggaca cgcgaagaac
     8701 gtcggcgatt tgtcttcgct gtcttcatgg acttccgaac aattcgaccc gcgcctgagc
     8761 tgggacgatg tggcgcgcat taaagatttg tggggcggca agctgattat caaaggcatt
     8821 atggaacccg aagacgcgga aaaagcagcg aaaagcggcg cggacgcatt ggtcgtttcc
     8881 aaccacggcg gccgacagct cgacgatacc gtgtctgcca tcaaagcctt gcccgacatc
     8941 gtcagcgcag tgggcagcga catcgaagtt tggatggaca gcggcatccg cagcggtcag
     9001 gatattttga aggcgtgggc tttgggcgcg aaaggtacaa tgataggtcg ggcgtttctg
     9061 tatggcttgg gcgcgtatgg agaagaaggc gtaacccgcg cgctggaaat cctatataag
     9121 gaaatggata tatctatggc gtttacgggg caccgcgata ttcaagatgt cgatgcctcg
     9181 attttaaggg gtaaagattg gggcagggaa acggtttaga tactgaaaaa tacgatgccg
     9241 tctgaacaca gggttcggac ggcattaaaa tttggctgta ttttaataat gaactatctg
     9301 acaaaacgcc cttttgtaaa ggaagttttg tcaaacgggg ttgcctgcaa tgcactactt
     9361 cattttttca agcgatgccg cacctaaagg gaatgactcc gtaaattgtc catttttctg
     9421 atgatcagta aaaaacggta aaccgatcaa acgataatgc ggtgtatctt tttgcaggat
     9481 cttatccatc ccatattcca cagtgatggc ttctaaaaat tcttctttcc atccatcagt
     9541 ttccactaaa tgctcacctt tgggctcaat gaaaatttgg taatgcaaaa agtcgttcac
     9601 accattggaa gaagattttt gtttatcttt cagcaataaa ataaagtccg gcataaatcc
     9661 ttcgccatcg gcaaagttat tcaatttaaa cacttcttca ttacggatta aatgaacgtc
     9721 atattgagat ttcaaatcgc ccaaacgcgc tgaaataaat tgaattaacg cttcttccaa
     9781 acccgttccg gcaaaattat tcatcacata ccaatcattt tgcgtggcaa tagtcgtttt
     9841 tacatcatct tttttgaccc atttttgttt tggcgtgccg aaaatttccc ataatttttt
     9901 aggtgtaaat tcttttgtac cgataaacgg catatcactt tcattcagat gtttttcaac
     9961 catttccaag attttcaggc agcctgcaag tttatcgtcc aggcgaacct gtttgtcttg
    10021 ccctaatcct aaaaattcaa tttgccagtc tttcaacaat ttattttgca attcattgcg
    10081 attttgaata ttgagtttgc tttgcaggcg gtcaaaatgg aataaagatt gactattttt
    10141 ccctttgata tgcagggctt tattgaaaat gtgccgctcc atttcactca tttttatggt
    10201 ttgagtaaaa ttattttgcg tgccaagttg cttgactttt tcatcatttt catcggctgt
    10261 aaattgcgtt tcctgcaaca gttgattgcc gtgaacttgg aatgaaagcg tttgcggatt
    10321 ggcttgcagg ctgtttgcat tattggtttt ggcgtttggg ttggggattt ttttattcgc
    10381 ccaaattaac aattctctaa aatccttatt agcggcaaat tcagatttga gtttaaatgt
    10441 tgccaatacc ttatcatcgt ctttttcagg caaataaccg tcttttcgca actcgttttt
    10501 cagttctgaa atatagcggg attgctcatc gtgcgtgtaa taaaacaatt cttccaagat
    10561 acgcaattcg tgttgcatat cgttgtcaaa tttgcgttta ttcggctgtt taccttcaaa
    10621 cgcaaatgga aaataacgca cgccacgacc aatcaactgc ttttccgata cggtggcggc
    10681 agccgttttg cctgattttt tatttgaacc gccgccgttt tgcccttcat acaaacgcac
    10741 aatatcaaac aaattcagaa cgtcccaacc ttcggttaat ctgtccaccg taaaaatggc
    10801 acgaatcgga ttatcagccg cttccaaatt attcagcaat ttttctgttt cgctgtcggt
    10861 tttttcggtt ttggttttgt tggtttcgga gttggtaata atcacattgt gtttttgata
    10921 attttgtttt acccaatctg ccaaatgtgc aaactcaacg cctttttcct gcataaattt
    10981 tagggcttgt tcagtgcggg ttttgccttg ttcgttggcg ttatcgctat cgttcaagct
    11041 tgttgaaaat gtagttaaaa acgaaaaatc aaccgcctgc acattttctg cccaatttaa
    11101 aaatgccaga taatccgctt ttgattcatc aatcgtctta cttctaaaca acatcacagg
    11161 cttgaaattg gcaatgccat atttcaaagc gatttgatgg cgataccaag caaataataa
    11221 agcgtgcaac acccgctctt tcttgcccag cgtactggat accaaattga tttctttggt
    11281 ataacctttt tgcaaaaatt cttttaagcc aaattttgtg atgattttat ccgcgtattt
    11341 ttgttgcacg tcagcatttt caggcagcgt ggcggtaaat tccaacagca cattttggct
    11401 gggattgcca tttttattga gtaacaattc caaaaccata tgctcccagc ctttgcgttc
    11461 aatttcggca ttgcctgcat tggcttttaa ttccacttcc aaatccaact cgccttgttt
    11521 tttgcctttg gtttgcgcgt ttaaatggtg tgcttcatcg cccagcatca caagattcaa
    11581 ttcgtgcaaa tccgccaatg tggtttggtt ttcccgttcg gtgtgaatat cgttatacag
    11641 cttttgaatg ctggtaaatt taatttcaat gccgtctgaa tgtgggctga acgtttccac
    11701 tttgcgaatg ggaattaccg tatcgccctg caaaatcttc tcggtaaata aaaattttgc
    11761 gtgtgtcggg tcaataaaat tattttccgt tttatccacg atattgtttt gattcacaaa
    11821 aaacagaaaa tgccgatacc ctttttcaaa ataatacaaa atcaatgccg ccatcatcat
    11881 cgttttgcct gcacctgttg ccatattgaa cagcaaatgc gttggcttat tttttaaatc
    11941 agggaaatcg tctagctttg aagtgcggtc aaaaatcaaa aagttttcaa gggcagattt
    12001 ttgccattca aaaaaatcat attttaagtt tttggacgta aattctggca tttcaagtaa
    12061 ttcaggcttg tattttttaa ccgctttacg ttcattttca atcacttcaa acaacgtttt
    12121 attattcgcc atcttttttc tccgcttgat tttttactga ttgatagaat gcacggctta
    12181 aggctttatc atcatcgttc aggcaatctg caaattgttc gtcatccatt tcggataatg
    12241 aaacatacat ttgatttaaa tccaacattt caagcaccat ttgtttttgt tcgtctaatg
    12301 gcaaagattg aaattcaggc tcttcaataa tttggctaaa ttcatttatg ctgacgttgt
    12361 atttcaagaa atagcgttcg cataaacctt caaacagtgt tttgatgccg tctgaatctt
    12421 cgcaagccaa aatttgttgt tttgcggttt cgttaaatgg agcaagttcg gcataaacaa
    12481 actcgccgcc accttgccaa ttcacggctt tggaaatccc acttgattcg tttactaaaa
    12541 tcttgtttaa ccgctttaca atagtgtcag tttgtttgtc tatttgctcg atacagatat
    12601 attggcgatt cattttatgg gcaacggctg ccgttgtacc gctaccaccg aaaaaatcca
    12661 aaacaatatc gtttggcttg gtatgcgctt ctaaaatccg ttttaacagt ttttcagatt
    12721 tttgtccttt aatttcctct aatcccagca tttttgatac gctttcgcct gaatagctga
    12781 caattgcaat actattcaaa tcatcatatt cattcgcatt ccaaacatct tctaacggat
    12841 aacgttcggg acggttgcta aaattaattt cttgcaggat tttctcggca gtttctttat
    12901 taataccgaa ttttgtaaat gtattttttg ttgccctatc taaatctact aataccttaa
    12961 aatgcttatt atcaatataa tatttgttaa aattcagatg attataatct tttgaataaa
    13021 ataaaatggt gtcatgatta cggatataat tggagacagt agttttatag cctgataccc
    13081 aaccaatacg ccaaataatt tcacgctgga aattgtattc accaaaaatc tcgtccatca
    13141 aaactttgca ataatgtact tcgtggtaat ctaagtttat ataaattgcg ccatcatctg
    13201 ccaataattc tctagcaatc tccaacctgt ttttcataaa tgtcagccaa gtagagcgat
    13261 taaatttatc gttatatgca aagctatcgg tttctgtgtt atacggcggg tcaatataaa
    13321 tcagcttgat tttgccccta aactgcttgg caagcgaatg cagggcaatc agattattgc
    13381 ctttgataat gagattttcg gcgggtgtgc cgtctgaatg tcgtttgatt tcgccaaccg
    13441 cttgtttgcc atctgtggtg tggcgagaga attttgagaa tgcttttgcg tcaaaaagcc
    13501 gatcgatttc atcaaaagca agcgtttgat taaaaaagat ttcttgtctc tttcgggtta
    13561 atttggtgta taa
//
LOCUS       DAWFHY010000058        12880 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.058, whole genome shotgun sequence.
ACCESSION   DAWFHY010000058 DAWFHY010000000
VERSION     DAWFHY010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12880
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.058"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(58..1368)
                     /locus_tag="WGQ68_001439"
     CDS             complement(58..1368)
                     /locus_tag="WGQ68_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEZ0715220.1"
                     /translation="MDTSKQTLLDGIFKLKANGTTVRTELMAGLTTFLTMCYIVIVNP
                     LILGETGMDMGAVFVATCIASAIGCFVMGFVGNYPIALAPGMGLNAYFTFAVVKGMGV
                     PWQVALGAVFISGLIFILFSFFKVREMLVNALPMGLKMSIAAGIGLFLALISLKGAGI
                     IVANPATLVGLGDIHQPSALLALFGFAMVVVLGYFRIQGAIIITILTITVIASLMGLN
                     EFHGVVGEVPSIAPTFMQMDFKGLFTVSMVSVIFVFFLVDLFDSTGTLVGISHRAGLL
                     VDGKLPRLKRALLADSTAIVAGAALGTSSTTPYVESAAGVSAGGRTGLTAVTVGVLML
                     ACLMFSPLAKSVPAFATAPALLYVGTQMLRSARDIDWDDMTEAAPAFLTIVFMPFTYS
                     IADGIAFGFISYAVVKLLCRRTKDVPPMVWIVAVLWALKFWYLG"
     gene            complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="WGQ68_001440"
     CDS             complement(1507..2685)
                     /gene="ftsZ"
                     /locus_tag="WGQ68_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEZ0715221.1"
                     /translation="MEFVYDVAESAVSPAVIKVIGLGGGGCNAINNMVANNVRGVEFI
                     SANTDAQSLAKNHAAKRIQLGTNLTRGLGAGANPDIGRAAAQEDREAIEEAIRGANML
                     FITTGMGGGTGTGSAPVVAEIAKSLGILTVAVVTRPFAYEGKRVHVAQAGLEQLKEHV
                     DSLIIIPNDKLMTALGEDVTMREAFRAADNVLRDAVAGISEVVTCPSEIINLDFADVK
                     TVMSNRGIAMMGSGYAQGIDRARMATDQAISSPLLDDVTLDGARGVLVNITTAPGCLK
                     MSELSEVMKIVNQSAHPDLECKFGAAEDETMSEDAIRITIIATGLKEKGAVDFVPARE
                     VEAVAPSKQEQSHNVEGMIRTNRGIRTMNLTAADFDNQSVLDDFEIPAILRRQHNSDK
                     "
     gene            complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="WGQ68_001441"
     CDS             complement(2804..4048)
                     /gene="ftsA"
                     /locus_tag="WGQ68_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEZ0715222.1"
                     /translation="MEQQQRYISVLDIGTSKVLALIGEVQDDDKINIVGLGQAPSRGL
                     RAGMVTNIDATVQAIRQAVNDAELMADTKITHVTTGIAGNHIRSLNSQGVVKIKDGEV
                     TQADIDRAIETAKAINIPPDQKILDAVVQDYIIDTQLGVREPIGMSGVRLDTRVHIIT
                     GASTAVQNVQKCIERCGLKSDQIMLQPLASGQAVLTEDEKDLGVCVIDIGGGTTDIAV
                     YMNGAIRHTSVIPAGGNLITKDLSKSLRTPLDAAEYIKIHYGVASCDTEGLGEMIEVP
                     GVGDRTSRQVSSKVLAAIISARIQEIFGVVLGELQKSGFPKEVLNAGIVLTGGVSMMT
                     GIVEFAEKIFDLPVRTGAPQEMGGLSDRVRTPRFSTAIGLLHAACKLEGNLPQPENGA
                     VQEREGGGGLLARLKRWIENSF"
     gene            complement(4134..4862)
                     /locus_tag="WGQ68_001442"
     CDS             complement(4134..4862)
                     /locus_tag="WGQ68_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEZ0715223.1"
                     /translation="MWDNAEAMERLTRWLLVMMAMLLAASGLVWFYNSNHLPVKQVSL
                     KGNLVYSDKKALGSLAKEYIHGNILRTDINGAQEAYRRYPWIASVMVRRRFPDTVEVV
                     LTERKPVARWGDHALVDGEGNVFEARLDRPGMPVFRGAEGTSAEMLRRYDEFSTVLAK
                     QGLGIKEMTYTARSAWIVVLDNGITVRLGRENEMKRLRLFTEAWQHLLRKNKNRLSYV
                     DMRYKDGFSVRYAYDGLPEKESEE"
     gene            complement(4852..5766)
                     /locus_tag="WGQ68_001443"
     CDS             complement(4852..5766)
                     /locus_tag="WGQ68_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003764621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEZ0715224.1"
                     /translation="MQNFGKVAVLMGGFSSEREISLDSGTAILNALKSKGIDAYAFDP
                     KETPLSELKAQGFQTAFNILHGTYGEDGAVQGALELLGIPYTGSGVAASAIGMDKYRC
                     KLIWQALGLPVPEFAVLHDDTDFDAVEEKLGLPMFVKPAAEGSSVGVVKVKGKGRLKS
                     VYEELKHLQGEIIAERFIGGGEYSCPVLNGKGLPGIHIIPATEFYDYEAKYNRDDTIY
                     QCPSEDLTEAEESLMRELAVRGAQAIGAEGCVRVDFLKDTDGKLYLLEINTLPGMTSH
                     SLVPKSAAVTGVGFADLCIEILKTAHVG"
     gene            complement(5879..7288)
                     /locus_tag="WGQ68_001444"
     CDS             complement(5879..7288)
                     /locus_tag="WGQ68_001444"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEZ0715225.1"
                     /translation="MMKNRVTNIHFVGIGGVGMSGIAEVLHNLGFKVSGSDQARNAAT
                     EHLGSLGIQVYPGHTAEHVNGADVVVTSTAVKKENPEVVAALEQQIPVIPRALMLAEL
                     MRFRDGIAIAGTHGKTTTTSLTASILGAAGLDPTFVIGGKLNAAGTNARLGKGEYIVA
                     EADESDASFLHLTPIMSVITNIDEDHMDTYGHSVEKLHQAFIDFIHRMPFYGKAFLCI
                     DSEHVRAILPKVSKPYATYGLDDTADIYATDIENVGAQMKFTVHVQMKGHEQGSFEVV
                     LNMPGRHNVLNALAAIGVALEVGASVEAIQKGLLGFEGVGRRFQKYGDIKLPNGGTAL
                     LVDDYGHHPVEMAATLAAARGAYPEKRLVLAFQPHRYTRTRDLFEDFTKVLNTVDALV
                     LTEVYAAGEEPIAAADSRALARAIRVLGKLEPIYCENVADLPEMLLNVLQDGDIVLNM
                     GAGSINRVPAALLELSKQI"
     gene            complement(7442..8509)
                     /gene="murG"
                     /locus_tag="WGQ68_001445"
     CDS             complement(7442..8509)
                     /gene="murG"
                     /locus_tag="WGQ68_001445"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEZ0715226.1"
                     /translation="MGGKTFMLMAGGTGGHIFPALAVADSLRARGHHVIWLGSKDSME
                     ERIVPQYGIRLETLAIKGVRGNGIKRKLMLPFTLYQTVREAQRIIRKHRVECVIGFGG
                     FVTFPGGLAAKLLGVPIVIHEQNAVAGLSNRHLSRWAKRVLYAFPKAFSHEGGLVGNP
                     VRADISNLPVPAERFQGCEGRLKILVVGGSLGADVLNKTVPQALALLPDNARPQMYHQ
                     SGRGKLGSLQADYDALGVKAECVEFITDMVSAYRDADLVICRAGALTIAELTAAGLGA
                     LLVPYPHAVDDHQTANARFMVQAEAGLLLPQTQLTAEKLAEILGSLNREKCLKWAENA
                     RTLALPHSADDVAEAAIACAA"
     gene            complement(8513..9838)
                     /locus_tag="WGQ68_001446"
     CDS             complement(8513..9838)
                     /locus_tag="WGQ68_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HEZ0715227.1"
                     /translation="MQTDWQVVFLKISEVLVKVGDGVHTLLLDRPIVRDGRKFDAPLL
                     WMVVLMTAFSLLMIYSASVYLASKEGGDQFFYLTRQAGFVVAGLIASGFLWFLCRMRT
                     WRRLVPWIFALSGLLLVVVLIAGREINGATRWIPLGPLNFQPTELFKLAVILYLASLF
                     TRREEVLRSMESLGWQSIWRGTANLIMSATNPQARRETLEMYGRFRAIILPIMLVAFG
                     LVLIMVQPDFGSFVVITVIAVGMLFLAGLPWKYFFVLVGSVLGGMVLMITAAPYRVQR
                     VVAFLDPWKDPQGAGYQLTHSLMAIGRGEWFGMGLGASLSKRGFLPEAHTDFIFAIIA
                     EEFGFFGMCVLIFCYGWLVVRAFSIGKQSRDLGLTFNAYIASGIGIWIGIQSFFNIGV
                     NIGALPTKGLTLPLMSYGGSSVFFMLISMMLLLRIDYENRRKMRGYRVE"
     gene            complement(9964..11301)
                     /locus_tag="WGQ68_001447"
     CDS             complement(9964..11301)
                     /locus_tag="WGQ68_001447"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEZ0715228.1"
                     /translation="MTFQNKKILVAGLGGTGISMIAYLRKNGVEVAAYDAELKPERVA
                     QIGKMFDGLVFYTGRLKDALDNGFDILALSPGISERQPDIEAFKQNGGRVLGDIELLA
                     DIVNRRGDKVIAITGSNGKTTVTSLVGYLCIKCGLDTVIAGNIGTPVLEAEWQREGKK
                     ADVWVLELSSFQLENTESLRPTAATVLNISEDHLDRYDDLLDYAHTKDKIFRGDGVQV
                     LNADDAFCRAMKRAGREVKWFSLEYEADFWLERETGRLKQGNEDLIATQDIPLQGLHN
                     ATNVMAAVALCEAVGLPREALLEHVKTFQGLPHRVEKIGEKNGVVFIDDSKGTNVGAT
                     AAAIAGLQNPLFVILGGMGKGQDFTPLRDALAGKAKGVFLIGVDALQIRRDLDGCDLN
                     MTDCATLEEAVQKAYAQAEAGDIVLLSPACASFDMFKGYAHRSEVFIGAFKAL"
     gene            complement(11386..11658)
                     /locus_tag="WGQ68_001448"
     CDS             complement(11386..11658)
                     /locus_tag="WGQ68_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEZ0715229.1"
                     /translation="MKQTVKWLAAALIALGLNQAVWADDVSDFRENLQAAAQGNAAAQ
                     YNLGAMYYKGRGVRQDRALAQEWYGKACQNRDQDGCGHYQRLKAGY"
     gene            complement(11773..12855)
                     /locus_tag="WGQ68_001449"
     CDS             complement(11773..12855)
                     /locus_tag="WGQ68_001449"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEZ0715230.1"
                     /translation="MFLWLAHFSNWLTGLNIFQYTTFRAVMAALTALAFSLMFGPWTI
                     RRLTALKCGQAVRTDGPQTHLVKNGTPTMGGSLILTAITVSTLLWGNWANPYIWILLG
                     VLLATGALGFYDDWRKVVYKDPNGVSAKFKMVWQSSVAIIAGLALFYLAANSANNILI
                     VPFFKQIALPLGVVGFLVLSYLTIVGTSNAVNLTDGLDGLATFPVVLVAAGLAIFAYV
                     SGHYQFSQYLQLPYVAGANEVAIFCTAMCGACLGFLWFNAYPAQVFMGDVGALALGAA
                     LGTVAVIVRQEFVLVIMGGLFVVEAVSVMLQVGWYKKTKKRIFLMAPIHHHYEQKGWK
                     ETQVVVRFWIITIVLVLIGLSTLKIR"
BASE COUNT         3055 a         3690 c         3259 g         2876 t
ORIGIN      
        1 tggcaaacaa aatgccgtct gaaaaccttt cagacggcat ttttaatatc gaatcaatca
       61 gcccaaatac cagaatttca gtgcccacaa tacggcaaca atccatacca taggcggaac
      121 gtctttggtg cggcggcata aaagtttaac cacggcatag ctgatgaagc cgaaggcgat
      181 gccgtctgca atcgaatagg taaacggcat gaagacaatg gtcaggaatg cgggtgcggc
      241 ttccgtcata tcgtcccaat caatatccct cgcactgcgg agcatctgcg tgccgacata
      301 aagcagggcg ggcgcggtgg caaaagcggg aacacttttc gccaaaggtg aaaacatcag
      361 gcaggcgagc atcaatacgc cgacggtaac cgccgtcagg ccggtccgtc cgcctgccga
      421 tacgcccgcc gcgctttcca cataaggcgt ggtggaagaa gtacccaaag ccgcacctgc
      481 cacaatggcg gtagagtctg caagcagtgc gcgtttcagg cggggcagct taccgtccac
      541 cagcagtccg gcacggtggg atatgccgac cagcgttccg gtactgtcaa atagatcgac
      601 caagaagaag acgaaaatca cactgaccat actgacggta aacagacctt taaaatccat
      661 ctgcataaag gtcggcgcaa tgctcggtac ttcgccgacc acgccgtgaa actcgttcaa
      721 acccatcagg ctggcaatga cggtaatcgt caaaatggta atgatgattg caccttgaat
      781 gcggaagtat cccaaaacaa ccaccatggc aaaaccgaac agtgccaaca acgcagacgg
      841 ttgatgaata tcgcccaaac cgaccaaggt tgccggattg gcaacgataa tgcctgcgcc
      901 tttcagggaa atcagtgcca aaaacaaacc gataccggca gcaatcgaca ttttcaaacc
      961 cataggcagt gcgttgacca gcatttccct gactttaaaa aagctgaaca ggataaaaat
     1021 cagaccggag atgaacaccg cacccaacgc aacctgccaa ggcacgccca tacccttaac
     1081 gacggcaaag gtgaaatagg cattcagccc catccccggt gcgagtgcaa tcggatagtt
     1141 gccgacaaaa cccataacaa aacagccgat ggcagacgcg atacaggtag cgacgaatac
     1201 cgcccccata tccatgccgg tctcgcccaa aatcagaggg ttgacgataa cgatgtagca
     1261 catcgtcaaa aaagttgtca aacccgccat caactcggta cgcaccgtcg taccgtttgc
     1321 cttcagctta aaaatcccgt ccaacagtgt ttgttttgaa gtgtccatat ctgaaagtct
     1381 gtatcaagtg gaaaaatctg aatccgccga ccgcaaaccg tacgaaattg caacatcttg
     1441 aaaaacatac ctcccaaacc ggacaaaccg attcgggagg cagcaggttt acgggcaaac
     1501 agcacattat ttgtctgaat tgtgttgacg acgcaaaatc gcagggattt caaagtcgtc
     1561 aagtacggac tgattgtcga aatccgcagc ggtaaggttc atcgtgcgga taccgcgatt
     1621 ggtgcggatc ataccttcga cattgtggct ttgctcctgt ttggacggag caaccgcttc
     1681 tacctccctt gccggaacaa aatcgaccgc gcctttttct ttcagaccgg tagcgataat
     1741 ggtaatccgg atggcatctt cgctcatggt ctcgtcttca gccgcaccga atttgcattc
     1801 caaatcggga tgcgcgcttt ggttgacgat tttcatgact tcggacaact cggacatttt
     1861 caagcaaccc ggagcagtcg taatattgac cagcacaccg cgcgctccgt ccaaggttac
     1921 atcgtccagc agcggactgg aaatggcctg gtcggtcgcc atacgcgcac ggtcgatacc
     1981 ttgggcataa cccgaaccca tcatagcgat accgcggttg ctcatcacgg ttttcacgtc
     2041 ggcaaagtcg aggttgatga tttcgctcgg gcaagttacc acttcggaaa tgcctgcgac
     2101 cgcatcgcgc aatacattgt cggcggcacg gaaggcttcg cgcatcgtta cgtcttcacc
     2161 caatgcagtc atcagtttgt cgttcgggat gatgatcagc gaatcgacgt gttctttcaa
     2221 ctgttccaac cctgcctgtg cgacatggac gcgcttacct tcatatgcga acggtcgggt
     2281 aaccacggca acggtcagaa tgcccaaaga cttggcaatc tcagcaacaa ccggcgcgga
     2341 accggtaccg gtaccgccgc ccataccggt cgtgataaac agcatattcg caccgcgaat
     2401 ggcttcttca atggcttccc ggtcttcctg ggctgccgca cggccgatat cgggattcgc
     2461 gcccgcgccc aaaccgcgtg tcagattcgt acccaactgg attctcttcg ccgcatggtt
     2521 ttttgccaga gactgcgcat ccgtattggc actgataaac tccacaccgc gcacattgtt
     2581 ggcaaccatg ttattgattg cattgcaacc gccgccgccc aagccgatta ctttaatcac
     2641 cgcagggctg actgccgatt ctgccacgtc gtaaacaaat tccattcaaa aactcctgct
     2701 cgccccattc agaggacggt ttaaataaat tattattcat tatataagaa gtatcttgct
     2761 gctggcaaaa tacttctcac ctgtcaaacg gcaatccacc tgttcagaag ctgttttcaa
     2821 tccaccgttt caatcttgcc aacaaaccgc cgcccccttc cctctcttgc actgcaccgt
     2881 tttccggctg cggcaagttt ccttccagct tgcatgctgc atgaagcagc ccgatagcgg
     2941 tagaaaaacg cggtgtgcgg acgcggtcgg acaaaccgcc catttcttgg ggtgcaccgg
     3001 tgcgtacagg caaatcgaag attttttcgg caaattccac aatcccggtc atcatggaca
     3061 caccgccggt cagaacgata cccgcattca gcacttcttt ggggaaaccc gatttttgca
     3121 gctcgcccag cactacgcca aaaatctcct gaatccgtgc actgatgatt gctgccagaa
     3181 ccttactgga aacctgacgc gatgtccggt cacccacgcc cggaacttca atcatctcac
     3241 ccaagccttc cgtatcgcat gatgccacgc cataatggat tttaatgtac tcggcggcat
     3301 cgagaggtgt tctcaacgat ttggacaaat ctttggtaat cagattacca ccggccggaa
     3361 tgacggacgt atggcggatg gcaccgttca tataaacggc aatatcggtc gttccgccac
     3421 caatgtcgat gacgcatacg ccgaggtctt tttcatcttc agtcagcacc gcctgcccgc
     3481 ttgccaacgg ctgaagcatg atttgatcgc ttttcaagcc gcaccgctcg atacattttt
     3541 ggacattctg cactgccgta cttgcaccgg taatgatgtg cacccgcgta tccaaacgca
     3601 caccgctcat accgatgggc tccctcacgc caagttgggt gtcgataatg tagtcttgaa
     3661 ccacagcatc gagaattttt tgatcgggcg ggatattgat tgcctttgcc gtttcaatgg
     3721 cgcgatcgat gtctgcctgc gtgacttccc cgtctttaat tttaaccaca ccttgcgaat
     3781 taagactgcg gatgtggttg cctgcgatac ctgtggtaac gtgggtaatt ttggtatccg
     3841 ccatcagctc ggcatcattg accgcctgcc tgatggcttg gacggtggca tcgatattgg
     3901 ttaccatgcc cgcgcgcaag ccccgtgaag gagcctgccc caaaccgacg atgttgattt
     3961 tgtcgtcatc ttgaacttcc ccgatcagtg cgaggacttt agacgtaccg atatccagta
     4021 cgctgatgta tctttgctgc tgttccattg ttcgtctgct cttaaaactg attgaaattt
     4081 gcgtcgcacc gtttcagacg gcacggtcgt aatctgtccg atacctgttc ccactattct
     4141 tcggattctt tttcgggtaa accgtcgtaa gcatagcgga ctgaaaatcc gtccttatac
     4201 ctcatatcca cataggataa ccgattttta tttttacgca acagatgctg ccacgcttcg
     4261 gtaaaaagcc ggaggcgttt catctcgttt tcccgtccga gcctgacggt gatgccgttg
     4321 tccaaaacga caatccacgc cgaacgtgcc gtataggtca tctctttgat gcccaaaccc
     4381 tgttttgcca aaacagtcga aaattcgtca taacggcgga gcatttcggc agacgttcct
     4441 tccgcgcctc tgaataccgg cattccgggt ctgtccaagc gggcttcaaa aacattgcct
     4501 tcgccgtcca ccaaggcatg gtcgccccaa cgcgcgaccg gcttgcgctc ggtcaggacg
     4561 acctcaaccg tgtcgggaaa acggcggcgc accatgaccg acgcaatcca cggataccgg
     4621 cggtaggcct cctgtgcgcc attgatgtcc gtcctcaaaa tattcccatg gatgtattct
     4681 ttcgccaaac tgcccaatgc cttcttatcg gaataaacca ggttgccctt cagcgacacc
     4741 tgcttgacgg gcagatgatt cgaattgtaa aaccaaacca gcccggacgc agcaagcagc
     4801 atcgccatca tgacaagcag ccagcgcgtc agccgttcca tcgcttcggc attatcccac
     4861 atgtgcggtc ttcaaaattt caatacataa atcggcaaaa cccacgcccg taacggcagc
     4921 ggatttcggt actaaactat ggctcgtcat accgggcagg gtgttgattt ccaacagata
     4981 gagtttaccg tcggtatctt tgaggaaatc gacgcgcacg cagccttccg caccgattgc
     5041 ctgcgcgccg cgaaccgcca gttcgcgcat caggctttct tcggcttcgg tcaaatcttc
     5101 cgaaggacat tgataaatgg tgtcgtcgcg gttgtacttg gcttcgtagt cgtaaaactc
     5161 ggttgcggga atgatgtgta tgccgggcag ccctttgccg ttcaggacgg ggcaggaata
     5221 ttcgccgccg ccgataaaac gttcggcaat gatttcgccc tgaaggtgtt tcaattcttc
     5281 gtaaacgctt ttcagacggc cttttccttt gacttttacc acgcctacgc tgctgccttc
     5341 ggccgccggt ttcacaaaca tcggcaggcc caatttttct tcgacggcat cgaaatcagt
     5401 gtcgtcgtgc aggacggcga actcgggaac gggcaatccc aatgcctgcc aaatcagttt
     5461 gcagcggtat ttgtccatgc cgatggcgga tgcggcgaca ccgctgccgg tatagggaat
     5521 gcccaacagt tccaatgcac cctgaaccgc cccgtcttcg ccgtaagtac cgtgaaggat
     5581 gttgaatgcc gtctgaaaac cttgtgcctt caattcagac aatggggttt ctttaggatc
     5641 gaaggcgtat gcgtctatgc ctttgctttt taaagcattc aaaatggcgg tgccgctgtc
     5701 cagcgagatt tctcgttcgc tggaaaaacc gcccatcaat acggccactt tgccaaaatt
     5761 ctgcattgtt tttattcttt cctgattgct ttatgcttgt tgccagaggt cgtctgaaac
     5821 ctgatttgcg gtttcagacg acctttatat gatgttccgt ctgtcaggcg ggtgtgcctc
     5881 aaatctgttt cgacaattcc agcagcgcgg cggggacgcg gttgatgctt cccgcgccca
     5941 tattcaacac gatgtcgccg tcctgcaaaa cgttcaacag catttcgggc agatcggcaa
     6001 cgttttcgca gtaaatcggc tcgagtttgc ccaacacgcg gatggcgcgg gcaagagcgc
     6061 gggaatcggc ggcggcaatc ggctcttcac cggcggcata aacttcggtc agcaccagcg
     6121 cgtcaacggt attgaggact ttggtaaagt cttcaaacaa atcgcgcgtg cgggtatagc
     6181 ggtgcggttg gaaggcgagt accaaacgtt tttccggata cgcgccgcgt gcggcggcaa
     6241 gggtcgccgc catttcgacg gggtggtgtc cgtagtcgtc caccaagagc gcggttccac
     6301 cgtttggcaa cttgatgtcg ccgtattttt ggaagcggcg gccgacacct tcaaagccga
     6361 gcaagccttt ttggatcgct tcaaccgatg cgccgacttc cagcgccacg ccgatggctg
     6421 ccaatgcgtt cagcacgttg tgtctgccgg gcatattcag cacgacttca aacgacccct
     6481 gctcatgtcc tttcatttga acatggacgg tgaatttcat ttgcgcgccg acgttttcga
     6541 tgtcggtggc gtagatgtcg gcggtatcgt ccaaaccgta agtagcataa ggtttgctca
     6601 ctttgggcaa aatcgcgcgg acgtgttcgc tgtcaataca caaaaaggct ttgccgtaga
     6661 agggcatacg gtggatgaaa tcgataaacg cctgatgcag tttttcgacg ctgtgcccgt
     6721 aggtatccat atggtcttcg tcgatattgg taatgacgga cataatcggt gtcaggtgca
     6781 gaaaggatgc atccgactcg tcggcttcgg caacgatgta ttcgcctttg cccaagcggg
     6841 cgttggtgcc tgcggcgttg agtttgccgc cgataacgaa agtcgggtca agtcctgccg
     6901 cgccgaggat ggaggcggtc aggctggtgg tcgtggtttt gccgtgcgtg ccggcaatgg
     6961 cgatgccgtc acggaagcgc atcaactccg ccaacatcag ggcgcgcgga ataacgggaa
     7021 tttgctgctc caacgcagcg acaacttcgg gattttcttt tttgacggcg gtagaggtaa
     7081 cgacgacatc cgcaccgtta acgtgttctg cggtatggcc gggataaact tgaatgccca
     7141 ggctgcccaa atgctcggta gcggcatttc gcgcctgatc cgaaccggaa actttaaaac
     7201 ccaaattgtg caagacttcg gcgataccgc tcatgccgac gccgccgata ccgacaaaat
     7261 ggatgttggt cactcgattt ttcatcataa tgttgcgttc cggtggattt cgatgcgtaa
     7321 aggcgttatt ttaaagggct gaccgtttgc gcgccatagt tttctgacaa atatatagcg
     7381 gattgaaata aaaaacatcc atgccgtctg aacggctttt cagacggcat ggttcggcag
     7441 tttacgccgc acacgcaatc gcggcttcgg caacgtcgtc cgcgctgtgc ggcaatgcca
     7501 aagtacgggc gttttctgcc catttgagac atttttcgcg gttcaggctg ccgaggattt
     7561 cggcgagttt ttccgccgtc aactgggttt gcggcaacag cagtcctgct tctgcctgca
     7621 ccataaaacg cgcgttggcg gtttggtgat cgtcaaccgc gtgggggtac ggaacgagca
     7681 acgcacccaa tcccgccgcc gtcaactcgg caatcgtcag cgcaccggca cggcaaatca
     7741 ccaaatcggc atcgcggtag gcggacacca tgtcggtaat aaattccacg cattcggctt
     7801 tcacgcccag cgcgtcgtaa tccgcctgca agctgcccag cttgccccgt cccgattggt
     7861 ggtacatctg cggacgcgca ttgtcgggca gcaaagccaa tgcctgcggt acggttttgt
     7921 tcaaaacgtc cgcgcccaaa ctgccgccga ccaccaaaat tttcagacgg ccttcacacc
     7981 cttggaagcg ttcggcaggc acgggcaggt tgctaatatc ggcgcggacg gggttgccga
     8041 ccaagccgcc ttcgtggctg aacgctttcg gaaaagcgta caacacccgc ttcgcccagc
     8101 gcgacaggtg gcggttggac aaacctgcca cggcgttttg ctcgtgaatc acaatcggca
     8161 cgcctaatag cttcgccgcc aaaccgccgg ggaaggtaac gaagccgccg aagccgatga
     8221 cgcactcgac acggtgtttg cggataatcc gctgcgcttc gcggacggtt tgatacaaag
     8281 taaacggcag catcagcttg cgtttgatgc cgttgccgcg cacgccttta atcgccagcg
     8341 tttccaagcg tatgccgtat tgcggcacga tacgctcttc catcgaatcc ttgctgccca
     8401 gccaaatcac atgatggccg cgcgcgcgca atgaatccgc caccgccagc gcggggaaaa
     8461 tgtgtccgcc cgttccgccc gccatcagca taaaggtttt accgcccatg atttactcca
     8521 cccgataacc gcgcattttc cggcggtttt cataatctat acgcaacagc agcatcatgc
     8581 tgatcagcat gaaaaagact gacgaaccgc cataggacat caacggcagc gtcagacctt
     8641 tggtcggcaa agcaccgatg ttcacaccga tattgaagaa actttggata ccgatccaaa
     8701 tgccgatacc cgaagcgata taggcgttga aagtcaaacc caaatcgcgc gactgcttgc
     8761 cgatggaaaa cgcccgcacc accagccagc cgtaacagaa tatcagcacg cacataccga
     8821 agaaaccgaa ttcttcggcg atgatggcaa aaataaaatc ggtatgcgct tccggcagaa
     8881 agccgcgttt gctcaaactc gcacccaaac ccataccgaa ccactctccg cgcccgattg
     8941 ccatcagaga gtgggtaagc tggtagccgg caccctgcgg gtctttccac gggtccaaaa
     9001 atgccactac ccgctgcaca cggtagggag cggcggtaat catcagcacc atcccgccca
     9061 agacgctgcc taccaggacg aaaaaatatt tccacggcaa tcctgccaaa aacagcattc
     9121 caacggcaat gacggtaatg acgacaaacg aaccgaaatc cggctgtacc attatcagca
     9181 ccaaaccgaa cgccaccagc ataatcggca ggatgatcgc ccggaaacgg ccgtacattt
     9241 ctaatgtttc acgacgtgcc tgcggattgg tggcggacat gatcagattg gccgtccccc
     9301 gccaaatcga ctgccaaccc aaactttcca tgctgcgcaa cacttcttca cggcgcgtga
     9361 acaggcttgc caaataaagg atgaccgcca gcttgaacag ctcggtcggc tggaaattca
     9421 acggacccaa aggtatccaa cgggtcgcgc cattgatttc gcgcccggca atcaatacga
     9481 ctaccagcaa caggccggat agggcaaaaa tccacggcac aagccgccgc catgtcctca
     9541 tcctgcaaag aaaccataaa aaaccgctcg ctatcaagcc ggcaacgacg aaccccgcct
     9601 gtctggtcaa atagaaaaac tgatcgccgc cttcttttga tgccaaatac acagaagccg
     9661 aataaatcat cagcaggctg aacgccgtca tcagcaccac catccacaaa agcggcgcgt
     9721 cgaatttcct gccgtcgcgc acaatcggcc tgtcgagcag cagagtgtgg acaccgtcgc
     9781 ccacttttac caatacttcc gaaatcttca aaaaaaccac ctgccagtct gtttgcacct
     9841 gccgcaaagg gcaaaaattt cagacggcag acaatgccgt ccgaacatac gatacatccc
     9901 aaatcggtat tctaaatctt tacttgccgc ccaacaatga cggcgtttgc gtttcagacg
     9961 gtatcacaaa gccttaaacg ccccgataaa cacttccgaa cggtgcgcat agcctttaaa
    10021 catatcgaag ctcgcgcagg cggggctgag cagcacgata tcgcccgctt cggcttgggc
    10081 atatgccttc tgaaccgctt cttccaaagt ggcgcagtcg gtcatattca gatcgcagcc
    10141 gtccaaatcg cggcggattt gcagcgcatc gacaccaatc aggaacacgc cttttgcctt
    10201 gccggcaagc gcgtcgcgca ggggcgtgaa gtcctgccct ttgcccatgc cgcccaaaat
    10261 gacgaatagc ggattttgca aaccggcaat cgcggcggct gtcgcgccga cgttcgtgcc
    10321 tttgctgtcg tcgatgaaca ccacgccatt tttctcgccg attttttcca cgcggtgcgg
    10381 caggccttga aaggttttga cgtgttcgag caatgcttcg cgcggcaaac cgacggcttc
    10441 gcacaaggca acggcagcca tcacgttagt ggcgttgtgc aagccttgca gcggaatatc
    10501 ttgcgtggca atcaaatctt cattgccttg tttcaggcga cctgtctcac gttccaacca
    10561 aaaatcggct tcgtattcca acgaaaacca tttcacctcg cgcccggcgc gcttcatcgc
    10621 acgacagaac gcatcgtccg cattcaaaac ctgcacgcca tcgccacgga aaatcttatc
    10681 cttggtatgc gcatagtcga gcaagtcgtc gtagcggtcg agatggtctt cggaaatgtt
    10741 cagcaccgtc gccgcagtcg ggcgcaggct ttcggtgttt tccagttgga agctggaaag
    10801 ctccaacacc cacacgtccg cctttttgcc ttcgcgctgc cattccgcct ccaaaaccgg
    10861 cgtgccgata ttgcccgcga taacggtatc cagcccgcac ttgatgcaga gatagccgac
    10921 caggctcgtt accgtggttt tgccgttgct gccggtaatc gcaatcacct tgtcgcctcg
    10981 gcggttcaca atatccgcca gcaattcgat atcacccaac acgcgcccgc cgttttgctt
    11041 gaacgcctcg atatccggct gccgctcgct gatgccgggg ctgagcgcca gaatatcaaa
    11101 accgttgtcc agcgcatctt tcagacggcc tgtgtaaaac accagcccgt caaacatctt
    11161 accgatttgc gccacgcgct cgggcttcaa ctccgcatca taagcggcaa cctccacgcc
    11221 gtttttgcgc aggtaggcaa tcatggaaat acccgtgccg ccaagtccgg cgacgaggat
    11281 ttttttgttt tggaaagtca ttttggtttg tcctaaaaca aatcatattg ggcaggaaga
    11341 tgtctgctcc tgctcaagcc gctttcagac ggcatcgcga gctgttcaat aacccgcttt
    11401 caggcgttgg taatggccgc agccgtcttg gtctctgttt tgacaagcct taccgtacca
    11461 ttcttgggca agggcgcggt cttggcgcac gccgcgtcct ttgtaataca ttgcgcccaa
    11521 attgtattgg gctgctgcat ttccctgtgc tgccgcctgc aagttttccc gaaaatccga
    11581 tacgtcatcc gcccacaccg cttggttcaa gcccaaggca atcagggcgg cggcaagcca
    11641 tttgactgtc tgtttcatgg ttttacttct gttttagtat aagccgccgt taacgatagg
    11701 gctgggcgga ttgtcgccgc aggtttattg cgcgttcaaa tgccgtctga aagatgttca
    11761 gacggcatag gttcagcgga ttttgagggt actcaagccg atcaatacca agacgatggt
    11821 gataatccaa aagcggacga caacttgggt ttctttccag cctttttgtt cgtagtggtg
    11881 gtggatgggt gccatcagga aaatgcgttt tttggttttc ttataccagc cgacctgaag
    11941 cataacggac acagcttcga cgacgaacag accgcccata atcactaaaa caaactcttg
    12001 gcggacgatg acggcaacgg taccgagcgc ggcacccaat gccaatgcgc cgacatcgcc
    12061 cataaagact tgcgcgggat aggcgttaaa ccacaagaaa ccgaggcacg cgccgcacat
    12121 ggcggtgcag aatatagcga cttcgttcgc gccggcgaca tagggaagct ggaggtattg
    12181 ggaaaattgg tagtgtccgc tgacgtaggc gaaaatggcg aggccggcgg caacgaggac
    12241 gacggggaag gtcgcaaggc cgtccaagcc gtcggtgagg ttgacggcat tggatgtgcc
    12301 gacgatggtc aggtaagaca acaccaaaaa gccgaccacg cccagcggca gggcgatttg
    12361 tttgaagaac gggacaatca aaatattgtt ggcggaattg gcggcaaggt aaaacaatgc
    12421 caaaccggcg ataatggcaa cgcttgactg ccacaccatt ttgaatttgg cggacacgcc
    12481 gttggggtct ttatagacga ctttgcgcca gtcgtcgtaa aaaccgagtg cgcccgtggc
    12541 gagcaatacg cccaagagaa tccagatata cgggtttgcc cagttgcccc acaacagggt
    12601 ggacacggta atggcggtca gaatcagcga accgcccatc gtcggcgtgc cgtttttgac
    12661 gaggtgggtt tgcggaccgt cggtacgcac tgcctgcccg catttgagcg cggtcagcct
    12721 gcgtatcgtc cacgggccga acatcaggga aaacgctaag gcggtcaacg ccgccatgac
    12781 ggcgcggaat gtggtgtatt gaaaaatatt cagaccggtt aaccagttgc tgaaatgtgc
    12841 gagccataaa aacatggggc ttcctttttt tgttttgtcg
//
LOCUS       DAWFHY010000059        12872 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.059, whole genome shotgun sequence.
ACCESSION   DAWFHY010000059 DAWFHY010000000
VERSION     DAWFHY010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12872
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.059"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(54..362)
                     /locus_tag="WGQ68_001450"
     CDS             complement(54..362)
                     /locus_tag="WGQ68_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715231.1"
                     /translation="MTNNVDLKVPAKRYFTLDELCGLLQISPYGFAQWQHNHGVVVGY
                     GGERYTRLDVVKLLKLQSTFAPYAEGAESGSDGNRPVTLQEIGDGLKDLLADLDKELC
                     "
     gene            complement(346..648)
                     /locus_tag="WGQ68_001451"
     CDS             complement(346..648)
                     /locus_tag="WGQ68_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002641098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HEZ0715232.1"
                     /translation="MTLTKAELADILVDKVSNVTKNDAKEIVELFFEEIRSTLASGEE
                     IKISGFGNFQLRDKPQRPGRNPKTGEEVPITARRVVTFHASQKLKSMVEHYYDKQR"
     gene            complement(722..3085)
                     /locus_tag="WGQ68_001452"
     CDS             complement(722..3085)
                     /locus_tag="WGQ68_001452"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEZ0715233.1"
                     /translation="MQFSYSWLKTQADTELSSDKLEHLLTMSGLEVEEAETAAPAFAG
                     VVIAEVKSVEKHPDADRLNVTRVDAGTGELVQIVCGAPNVKAGIKVPCSLPGAVLPGN
                     FKIKPTKMRGVVSNGMLCSTDELGLPDGGVNGLHILPQDAPVGVNIREYLDLDDTVFT
                     LKITPNRADCLSIKGIAREVSALTGCAFRQPEIHTAPITGSRKQPVQINAPADCGRFI
                     SRVIENVNAKAATPDWMKQRLERSGIRSISALVDIGNYVMLEIGQPMHVFDADKLSGS
                     LHIRRAREGETLECLNEKTVSLSENTLVVADEKGVLSLAGLMGGAASAVSDGTQNIVL
                     EAAWFAPEIIAGKSRQYGFGSDSSFRFERGVDYRLQADAIERATELVLQICGGAAGEM
                     VEAQGELPEAKQVGLRLDRLKTVLGVDIPAEQVETILQHLGLQPEKTAEGFRVTAPSF
                     RFDIEIEADLIEEIGRVYGYENIPDDYTSGRLKMLELPETRRPRFAVYNEMAARGYRE
                     VVSYAFVDEQWEQDFAANADPIRLQNPLAAQYAVMRSTLIGGLVEILQNNLNRKQNRV
                     CVFEIARVFGKGSDGRFVQNERIGSLWYGAAMPEQWGGKTRNADFYDIKADVENLLKN
                     KAVEFVKTGHPALHPGRAANIVSDGKVIGFVGELHPKWLQKYDLPQAPLVFEIDMAVV
                     LECGKTRYRAVSKFQPVRRDLAFVMPEAMSHDDLLLVLKGAANKLVQEISVFDVYRGT
                     GLPEGMKSVAVKVILQDMENTLTDEAVEPLIGKLIGAATAAGARLRS"
     gene            complement(3121..3639)
                     /locus_tag="WGQ68_001453"
     CDS             complement(3121..3639)
                     /locus_tag="WGQ68_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715234.1"
                     /translation="MSEIINLTESGITFNLVKNNLFYIEPYIRKRRQEGIKTVEFIYL
                     QEDNLLFVEAKQSIPNQERSPERFDEYISEIYQKWCNALNVEILGILGREDIKETIMP
                     SAFSNLQWGSIEIKLLLVIPDVPLNYLGQLNELIKQEFNKKDTLRLISLWNISVEVIN
                     RDLAIQKGLASS"
     gene            complement(3646..4710)
                     /locus_tag="WGQ68_001454"
     CDS             complement(3646..4710)
                     /locus_tag="WGQ68_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEZ0715235.1"
                     /translation="MIKSFWLENFGPISLLKADNLGKINLIIAENSKGKTFILKALYS
                     VLKSHEEAHKGKNIRDFSEELRDKLYWTFQVEEIGDLVTRGKENPNERPLKLSMTLED
                     SSSVLFSFGRTTKKLIKPELYELSPRINANSIFLPPKEVLSLFDVIKKSEEEKRFGFD
                     ATYIDLVKALDIKPTKGRNYPEAAEARKDLEALFGGYVSYNDKKKAWVYEKDRQTFSI
                     NSTAEGVKKIGILDTLLGNRFLSPGSILFIDEPEANLHPKAINQFLEILFSLSKSKLG
                     IQIFMSTHSYFVIKKLCLLARKHSESIPVFMPGKEKEQTTWVQENLQNSMPDNEIIGE
                     SIRLFREEIGVLSPETAWIG"
     gene            complement(4887..5879)
                     /gene="pheS"
                     /locus_tag="WGQ68_001455"
     CDS             complement(4887..5879)
                     /gene="pheS"
                     /locus_tag="WGQ68_001455"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0715236.1"
                     /translation="MENVNRIVAEGIAAVEAAQDFNALEQIKARYLGKTGELTGFLKT
                     LGQMSPEERKTIGAHINECKNRFQTAFNAKRDALNEAKLQARLAAEALDITLPGRAQE
                     GGSLHPVTLTLQRVVELFHGMGFEVADGPEIEDDFHNFQALNIPANHPARAMQDTFYV
                     ENGDVLRTHTSPIQIRYMLDKKEPPIRIIAPGRVYRVDSDATHSPMFHQAEGLWVEEG
                     VTFADLKAVFTDFIRRFFERDDLQVRFRPSFFPFTEPSAEIDIMGENGKWLEVGGCGM
                     VHPNVLRNVNIDPEKYTGFAFGIGLDRFAMLRYNVNDLRLFFDNDLNFLKQFVK"
     gene            complement(6225..6584)
                     /gene="rplT"
                     /locus_tag="WGQ68_001456"
     CDS             complement(6225..6584)
                     /gene="rplT"
                     /locus_tag="WGQ68_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEZ0715237.1"
                     /translation="MPRVKRGVTARARHQKIFALAKGYRGRRKNVYRVAKQAVMKAGQ
                     YAYRDRRQRKRQFRQLWIVRINAGTRENGLSYSKFMNGLKRASIEIDRKVLADLAVFD
                     KAAFAQLVEKAKAALAA"
     gene            complement(6597..6794)
                     /gene="rpmI"
                     /locus_tag="WGQ68_001457"
     CDS             complement(6597..6794)
                     /gene="rpmI"
                     /locus_tag="WGQ68_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEZ0715238.1"
                     /translation="MPKMKTKSSAKKRFKVLGNGGVKRAHAFKRHILTKKTTKNKRQL
                     RGTSMVNDRDLASVAKMLPYA"
     gene            complement(6941..7462)
                     /gene="infC"
                     /locus_tag="WGQ68_001458"
     CDS             complement(6941..7462)
                     /gene="infC"
                     /locus_tag="WGQ68_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEZ0715239.1"
                     /translation="MIAQEREARINGEITAKEVRLISESGEQLGVVSVREALAMAEGQ
                     DVDLVEISPTAKPPVCKLMDYGKYKYQQAKKRDEAKKNQKQVQIKEIKFRPGTDEGDY
                     QIKMRNINRFLADGDKVKVTLRFRGREMAHQQLGAQLLERVKEDLAEVAQIESFPKME
                     GRQMVMMIAPKKK"
     gene            complement(7480..9393)
                     /gene="thrS"
                     /locus_tag="WGQ68_001459"
     CDS             complement(7480..9393)
                     /gene="thrS"
                     /locus_tag="WGQ68_001459"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEZ0715240.1"
                     /translation="MLNITLPDGSVRQYESPVTVAQIAASIGVGLAKAAVAGRVNGKL
                     VDACDPIVEDSAVQIITPKDQEGIEIIRHSCAHLVGHAVKQLYPNAKMVIGPVIEEGF
                     YYDIATEKPFTPEDVAAIEARMKELIAQDYDVVKIMTPRAEAIKIFQERGEEYKLRLI
                     DDMPEVEAMGMYHHQEYVDMCRGPHVPNTRFLKNFKLTKLAGAYWRGDSNNEMLQRIY
                     GTAWATKDELKAYIQRIEEAEKRDHRKLGKQLDLFHLQDEAPGMVFWHPKGWALWQVI
                     EQHMRKELNAAGYKEVKTPQIMDKTFWEKSGHWDNYKDNMFVTSSEKREYAVKPMNCP
                     GHVQIFNNGLRSYRDLPMRLAEFGSCHRNEPSGALHGLMRVRGFVQDDAHIFCTEDQI
                     VSEARAFNELLVRIYKQFGFHDVAVKLSLRPEQRAGSDEVWDKAEQGLRDALTACGVE
                     WEELPGEGAFYGPKIEYHVKDALGRSWQCGTLQLDFVLPERLDAEYVTENNDRARPVM
                     LHRAILGSLERFIGILIENHAGSFPLWLAPVQMVIMNITENQADYCREVAAKLQAAGF
                     RAELDLRNEKIGYKIRDNSQYRFPYQIVVGDKEKQENKVAVRRKAEDLGSLDLDDFIA
                     QLQQEITDALVNH"
     gene            complement(9447..9523)
                     /locus_tag="WGQ68_001460"
     tRNA            complement(9447..9523)
                     /locus_tag="WGQ68_001460"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9487..9489),aa:Val,seq:gac)
     gene            9702..10817
                     /gene="tgt"
                     /locus_tag="WGQ68_001461"
     CDS             9702..10817
                     /gene="tgt"
                     /locus_tag="WGQ68_001461"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEZ0715241.1"
                     /translation="MLKFTLHKKDGLARRGTLELNHGKIETPVFMPVGTYGSVKAMNP
                     QNLHDIKAQIILGNTYHLWLRPGLEVVEQFGGLHGFIGWDKPILTDSGGFQVFSLSDM
                     RKLTEEGCTFKSPINGDKLFLSPEISMKIQTVLNSDIAMQLDECTPGEATREQAKKSL
                     QMSLRWAERSKKAFEDLKNPNALFGIVQGAMYEDLREESLRGLEQFDFPGLAVGGLSV
                     GEPKPEMYRMLRAVGPILPEHKPHYLMGVGTPEDLVYGVAHGIDMFDCVMPTRNARNG
                     WLFTRFGDLKIKNAKHKLDKRPIDESCTCYACQNFSRAYLHHLHRAGEILGAQLNTIH
                     NLHFYQVIMAEMRDAIEQGKFADWQARFHENRARGTD"
     gene            10899..11909
                     /locus_tag="WGQ68_001462"
     CDS             10899..11909
                     /locus_tag="WGQ68_001462"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEZ0715242.1"
                     /translation="MLPFFPEPSLSYTQQNRTAVLLLNLGTPDAPTAQAVRPYLKSFL
                     TDRRVVELPKWLWYPILHGLVLTLRPKKSAHAYEKIWFKEGSPLEVYTARQAAALAER
                     MPDLIVRHAMTYGNPSVADVLSELKAQGAGRLLVIPMYPQYAASSSGAAVDKVCEQLL
                     LQRNQMSVRTVSRFYDDTGYIDAMKNHILRYWAEHGRGKKLMLSFHGVPQKHYDLGDP
                     YPDECRHTAKLLAEALELTEDQYVVSFQSQFGRAKWVTPSTQDLFGKLPKQGVTELDV
                     FCPGFLADCLETMEEIALMGREQFYEAGGKSYRYIPCLNDNPDWIDALVALAEGNLGG
                     WR"
     gene            12100..12501
                     /locus_tag="WGQ68_001463"
     CDS             12100..12501
                     /locus_tag="WGQ68_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0715243.1"
                     /translation="MNTTRLPTALVLGCLCAAASAADNSIMTKGQKVYESNCVACHGK
                     KGEGRGTMFPPLYRSDFIMKKPQVLLHSMVKGINGTIKVNGKTYNGFMPATAISDADI
                     AAVATYIMNAFDNGGGSVTEKDVKQAKGKKN"
     gene            complement(12564..12854)
                     /locus_tag="WGQ68_001464"
     CDS             complement(12564..12854)
                     /locus_tag="WGQ68_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715244.1"
                     /translation="MAKYFTETGISVREYFDFFGEFVVLPAARSGDLALAYGLRLEAG
                     EEGLSLAELFDKRSDSQEPVECGRIDIGGFMLTAKEVDGGGNIGSMGLKVPR"
BASE COUNT         3264 a         3394 c         2862 g         3352 t
ORIGIN      
        1 tattttttag cgtgtaaaac aaaaccggct gcatacctgc aaccggcctc aaatcagcac
       61 aattccttat ccaaatccgc caacaggtct ttcagaccgt ctccgatttc ctgaagcgta
      121 accggacggt tgccgtccga acccgattcc gcaccttctg catacggtgc aaacgtgctc
      181 tgcaatttca acagtttcac cacatccaaa cgggtgtagc gttcgccgcc gtaaccgaca
      241 accacaccgt gattatgctg ccattgcgca aaaccatagg ggctgatttg caacagtccg
      301 cacaactcgt ccagcgtgaa atagcgtttt gcgggaacct tcaaatcaac gttgtttgtc
      361 atagtagtgt tccaccatgc ttttgagttt ctggctggca tggaaagtta ccacgcggcg
      421 ggcggtaatc ggcacttcct cgccggtttt aggattacga cccgggcgtt gcggcttgtc
      481 gcgcaactgg aaatttccga aaccggaaat tttgatttct tcgccgcttg ccaaagtgct
      541 gcggatttct tcaaaaaaga gttcgacgat ttctttggca tcgtttttgg tgacgttgct
      601 gactttgtct accaaaatat cggccagttc tgctttagtg agagtcatat gattactttc
      661 tgttgtaaca gttgtggatt attaccaatt tttatttaaa attcaagcga atatttgttt
      721 tttaactgcg aagccgcgcc cctgccgccg ttgccgcgcc aatcagtttt ccgataagcg
      781 gctcgaccgc ctcatccgtc agcgtgtttt ccatatcctg caaaatcact ttgaccgcca
      841 cgctcttcat cccttcgggc aggcccgtgc cgcgatacac gtcaaacacg ctgatttcct
      901 gtaccaactt gtttgccgcg cctttcaaga caagcagcaa atcatcatgg ctcatagctt
      961 ccggcatgac aaacgccaaa tcgcggcgta ccggctggaa tttcgatacg gcccgatagc
     1021 gcgttttccc gcattccaac acaaccgcca tatcgatttc aaataccagc ggcgcttgcg
     1081 gcaggtcgta tttttgcagc catttcggat gcagttcgcc gacaaagccg atgacttttc
     1141 cgtctgaaac gatattggcg gcacgtccgg gatgcagggc gggatgtccg gttttaacga
     1201 actcgactgc tttgtttttc aacagatttt ccacgtccgc cttgatgtcg taaaaatccg
     1261 cattgcgcgt tttcccgccc cattgttcgg gcattgccgc gccgtaccac agactgccga
     1321 tgcgttcgtt ttggacaaac cggccgtctg aacctttgcc gaacacgcgg gcgatttcaa
     1381 acacgcacac gcggttttgt ttgcggttca ggttgttttg caaaatttcc accaagccgc
     1441 cgatgagtgt ggaacgcatc acggcatatt gcgccgccaa cgggttttgc aggcggatgg
     1501 ggtcggcgtt ggcggcaaaa tcttgttccc actgctcgtc aacgaaggca tagctgacca
     1561 cttcgcggta accgcgagcc gccatttcgt tgtaaacggc aaaacgcggg cggcgtgttt
     1621 cgggcagttc cagcattttc agacggcctg acgtgtaatc gtcggggatg ttttcatagc
     1681 cgtaaacgcg tccgatttct tcaatcaaat cagcctcaat ttcgatgtca aaacggaagc
     1741 tcggcgcggt aacgcggaag ccttccgccg ttttctcggg ctgcaggccc aagtgttgca
     1801 aaatggtttc cacctgttcg gcaggaatgt ccacgcccaa cacggttttc agacggtcca
     1861 aacgcaatcc aacctgcttc gcttcaggca attcgccttg cgcttccacc atctcgcctg
     1921 ccgcaccacc gcaaatctgc aacaccaatt cggtagcacg ttcaatggca tccgcctgca
     1981 aacggtaatc cacgccgcgc tcgaagcgga acgacgaatc cgaaccgaaa ccgtattggc
     2041 gcgatttgcc ggcgatgatt tcgggcgcaa accaagccgc ttccagcacg atattttgcg
     2101 tgccgtctga aaccgcgctc gccgcgccgc ccattaagcc cgccaaactc aacacgcctt
     2161 tttcgtccgc cacgaccagc gtgttttcag acagggaaac ggttttctcg ttcaggcatt
     2221 ccagcgtttc cccttcgcgc gcgcggcgga tgtgcaggct gccggaaagt ttgtcggcat
     2281 caaaaacgtg catcggctga ccgatttcca gcatcacata attgccgatg tccaccagcg
     2341 cggaaatact gcggatgccg ctgcgctcca aacgttgttt catccaatcg ggagtagccg
     2401 ctttcgcatt gacattttca ataacgcggc tgataaaacg gccgcaatcg gcaggcgcgt
     2461 taatctgcac gggctgtttt cgactgcccg tgatcggcgc ggtatggatt tcgggctgcc
     2521 tgaacgcgca ccccgtcaat gcggacactt cgcgcgcaat gcctttgatg ctcaggcagt
     2581 cggcgcggtt cggcgtaatt ttcagcgtaa acaccgtgtc gtccaaatcc agatattcgc
     2641 ggatgtttac accgacagga gcatcttgcg gcagaatatg caggccgttc acaccgccgt
     2701 cgggcagacc gagttcatcg gtggaacaca gcatcccgtt cgacaccacg ccgcgcatct
     2761 tggtcggctt gattttgaaa ttgcccggca acaccgcgcc cggcaacgaa cacggcactt
     2821 tgatgcccgc tttcacattc ggcgcaccgc acacgatctg aaccaactct cccgtacccg
     2881 catcaactcg ggtaacgttc aaacggtcgg catcaggatg tttttcaacg gatttcactt
     2941 cggcaatcac cacgcccgca aacgcaggcg cggcagtttc ggcctcttcc acttccaagc
     3001 cggacatcgt taacagatgt tccagcttat cggaggaaag ttcggtatcg gcttgggttt
     3061 tcagccatga gtaggagaat tgcatgttat gttctcaatt aactaattaa ctttagattt
     3121 tcaagagcta gctaaacctt tttgaatggc taagtcccta ttaattactt ctacagaaat
     3181 gttccataaa gaaatcaacc gcaaagtatc tttcttatta aattcttgct ttattaattc
     3241 attcaattgc ccaagataat taagcggcac atccgggata accagtaata atttgatttc
     3301 tatgctaccc cattgcaaat ttgaaaaagc actaggcata attgtttctt tgatatcttc
     3361 tctgcctaat attcccaaaa tttcaacatt taatgcatta caccattttt gataaatctc
     3421 tgaaatatat tcatcaaaac gctcaggaga tctttcttga tttggaatac tttgttttgc
     3481 ctcaacaaat aacaaattat cttcttgcag atagataaac tctactgtct taataccttc
     3541 ttgccttctc ttccggatat atggttctat ataaaacagg ttatttttta ctagattaaa
     3601 agttatacct gattcagtga gatttattat ttcactcata aaaacctaac caatccatgc
     3661 tgtttccgga gataaaacac ctatttcttc tctgaacaat cgaatagact caccaataat
     3721 ttcattatcc ggcatgctat tttgtaaatt ttcctgcacc caagtagtct gttccttttc
     3781 ttttccaggc ataaaaacag gaatactttc actatgcttt ctagctaata aacacagctt
     3841 cttgataaca aaatatgaat gcgtactcat aaaaatctgt attcccaatt tggatttaga
     3901 taaggaaaac agaatttcta gaaattgatt aattgctttc ggatgtagat tagcttctgg
     3961 ctcatcaata aataagattg atccaggact caaaaaccga tttcctaata gagtgtctaa
     4021 tatcccaatc ttttttaccc cctctgctgt agaattgata gagaaagttt gtctatcttt
     4081 ttcatatacc catgccttct ttttatcatt gtagctaaca taaccgccaa acaatgcttc
     4141 tagatccttt ctagcttccg ctgcctcagg gtaattcctc ccttttgttg gtttaatatc
     4201 caatgctttt accaaatcaa tgtaagtagc atcaaaacca aatctctttt cttcctctga
     4261 ttttttaatt acatcaaata gtgatagaac ttctttgggt ggtaaaaaaa tagagttagc
     4321 gtttattctg ggagaaagtt cgtatagctc cggtttaatt agctttttgg tagttctacc
     4381 aaaagaaaac aaaactgaag aactatcttc taaagtcata cttaacttaa gaggccgttc
     4441 gtttggattt tcttttcctc ttgtaactaa atcaccaatt tcctctactt ggaacgtcca
     4501 atatagctta tctcgcaatt cctcagaaaa atcacgaata ttctttcctt tatgagcttc
     4561 ctcatggctt tttaaaacac tatataatgc ctttaaaata aaagttttcc ctttgctatt
     4621 ttctgcaata attaaattta ttttccctaa attatcagcc ttcaataagc taatcggccc
     4681 gaaattttca agccaaaatg atttaatcat ataatccccc taatatatat caacaatatt
     4741 tgtacaagac acattgtgaa aagacagcaa ccacagcacc atagtaaacg catagcaggc
     4801 ggataatgga gcgacatagc gtttcatgat ttagctttct gaggtaggca caggctgctt
     4861 ttttcatttt taggcagcct acacaatcat tttacaaact gcttcaaaaa attcaaatcg
     4921 ttatcaaaaa acaagcgcaa atcgttcacg ttgtaacgca gcatcgcgaa acggtcgaga
     4981 ccgataccga aagcgaaacc ggtgtatttt tcgggatcga tattcacatt tctcaacacg
     5041 ttgggatgca ccataccgca gccgccgact tccagccatt tgccgttttc gcccataatg
     5101 tcgatttcgg cagacggttc ggtgaacggg aagaaagacg ggcggaaacg tacttgcaaa
     5161 tcatcgcgtt caaagaagcg acggataaaa tccgtgaaca ctgcttttaa gtcggcaaaa
     5221 gttacgccct cttctaccca caaaccttcc gcctgatgga acataggcga gtgcgtggca
     5281 tcgctgtcca cacggtaaac gcggccgggg gcgataatgc ggatgggcgg ctctttttta
     5341 tcgagcatat agcggatttg aatcggggaa gtgtgcgtac gcaaaacatc accgttttca
     5401 acgtaaaacg tatcctgcat cgcacgggca ggatggtttg cagggatgtt cagggcttgg
     5461 aaattgtgaa aatcgtcttc gatttcaggc ccgtccgcca cttcgaaacc cattccgtga
     5521 aagagttcga ccacacgttg caaggtcagg gttacgggat gcaggctgcc gccttcctga
     5581 gcgcgtccgg gcagggtaat gtcgagggct tcggcggcaa gtcgggcttg cagcttggct
     5641 tcgttgaggg catcgcgttt ggcattaaaa gccgtctgaa accggttttt gcattcattg
     5701 atatgcgcac ctatggtttt gcgctcttca ggcgacattt gccccaaagt tttcagaaat
     5761 ccggtcaact cgccggtttt gccaagataa cgggctttga tttgttctag agcgttgaag
     5821 tcttgcgcag cttctactgc ggcaatgcct tctgcaacga tgcggtttac attttccata
     5881 atatcaagtc tgtcaattaa tgatatgcca cctgaaacaa cgaggtcgcc tgtatagcct
     5941 gacatatcag actcttcaga cgaccttatt ttcttcacgg tcaatccatt ggataaattt
     6001 tctgtatagt ggattaaatt taaaccagta cggcgttgcc tcgccttgcc gtactatccg
     6061 tactgtctgc ggcttcgtcg ccttgtcctg atttaaattt aatccactat attttaacgc
     6121 aaatttattt aaaaaaatag actttaaaga aagaaaatca atcacataga atttctacaa
     6181 acaaagaaaa aaggaagccg cagcttcctt ttcaattttt tggattaagc agccaaagca
     6241 gctttggctt tttcaaccaa ttgtgcaaaa gcggctttat cgaacacggc caaatcagcc
     6301 aatactttgc ggtcgatttc gatagaggcg cgtttcagac cgttcataaa tttgctgtaa
     6361 gacaacccgt tttcacgcgt acctgcattg atacggacaa tccacaattg acggaattgg
     6421 cgtttgcgtt ggcggcggtc acggtatgcg tattgaccgg ctttcattac cgcctgcttg
     6481 gcaactcggt aaacgttttt acgacgaccg cgatagcctt tggctaacgc gaagattttt
     6541 tggtgacggg cacgggcggt aacaccgcgt tttacgcgtg gcatattcta aactccttaa
     6601 gcgtagggta acattttagc aacagaagcc aaatcgcgat catttaccat agaggtaccg
     6661 cgcaattggc gtttgttttt ggtggtcttt ttagtcaaga tgtggcgttt gaacgcatga
     6721 gcgcgtttca caccgccgtt acccagtact ttaaagcgtt ttttcgcgct agacttggtt
     6781 ttcattttag gcatgggaaa actccattcg ttatcggata aggcattagg gggttttaaa
     6841 acatcggttt caaacacttg aacccacaat gccacggttt tttgccgcaa ttaaaaactt
     6901 actccgaagc gacaatcgga gtaagcggag aattatagct ttattttttc ttcggcgcaa
     6961 tcatcatcac catttggcga ccttccattt tgggaaagga ctcgatttgc gccacttcag
     7021 ccaaatcttc ttttacacgt tccaaaagtt gcgcgccgag ttgctggtga gccatttcac
     7081 ggccgcggaa acgcaatgtc actttgactt tatcgccgtc ggcaaggaag cggttaatgt
     7141 tgcgcatctt gatttgataa tcgccctcat ccgtacccgg acggaatttg atttccttga
     7201 tttgcacctg cttttggttt ttcttggctt cgtcgcgttt cttggcctgc tggtatttgt
     7261 atttaccgta atccatcagt ttgcacacag gcggtttagc agttggggaa atctctacca
     7321 aatcgacatc ctgcccttcg gccatagcca aagcttcacg aactgaaacg acaccaagct
     7381 gttcgcctga ctcactgatt aaacgcactt ctttggcggt aatttcgccg ttgattcgtg
     7441 cttcgcgttc ttgagcgatg atgaatactc ctataaaaat taatgattga cgagggcatc
     7501 agtgatttct tgctgcaatt gcgcaatgaa atcatccaaa tccaaagaac ccaaatcttc
     7561 tgctttgcgg cgtaccgcca ctttgttttc ctgcttttct ttatcgccga caacgatttg
     7621 ataaggaaaa cggtattggc tgttgtcgcg gattttgtaa ccgatttttt cgttacgcaa
     7681 atccaactcg gcgcggaatc ctgccgcctg caatttggca gccacttcac ggcagtaatc
     7741 cgcttgattt tcggtgatat tcataatcac catttgaacc ggcgccagcc ataacgggaa
     7801 agagcctgca tggttctcaa tcagaatgcc gataaaccgc tccaaagaac ccaaaatggc
     7861 gcggtgcaac attacagggc gagcgcggtc attgttttcg gttacatatt ctgcatccaa
     7921 gcgttccggt aagacgaaat ctagttgcaa tgtaccgcat tgccaagaac gacccaaggc
     7981 atctttgacg tgatattcga ttttaggccc gtaaaacgca ccttcgccag gcaactcttc
     8041 ccactctacg ccacaagcgg tcaatgcatc acgcaagcct tgttcggcct tatcccaaac
     8101 ttcgtcagaa cctgcacgtt gttcagggcg gagggacaac ttcacagcaa catcatggaa
     8161 accgaactgt ttgtagatgc gaaccaacaa ttcattgaac gcacgggcct cgctgacgat
     8221 ttggtcttcg gtacagaaaa tatgcgcatc atcctgcaca aatccacgga cccgcatcag
     8281 accatgcaat gcaccgcttg gttcattgcg gtggcaagaa ccgaattccg ccaaacgcat
     8341 cggcaaatcg cgatatgagc gcaaaccgtt attaaaaatt tgcacatgac ccggacagtt
     8401 catcggttta accgcatatt cgcgtttttc cgaactggtt acgaacatat tgtctttgta
     8461 gttgtcccaa tggccggatt tttcccaaaa ggttttatcc atgatttgag gcgttttgac
     8521 ctctttataa ccggcggcgt tcagttcttt acgcatatgc tgttcaatca cttgccacaa
     8581 agcccagcct ttaggatgcc aaaacaccat gcccggcgct tcgtcttgca gatggaacag
     8641 atccaattgc ttgccaagtt tgcggtggtc gcgcttttcg gcttcttcga tacgttgaat
     8701 ataggctttc aactcgtctt ttgtcgccca agccgtaccg tatatgcgtt gcagcatttc
     8761 attgttgctg tcgccgcgcc agtatgcgcc cgccaacttg gtcagtttga agtttttcag
     8821 gaaacgggta ttcggaacgt gcgggccacg gcacatatcg acatattcct ggtgatgata
     8881 catccccatc gcttccactt cgggcatatc gtcaatcagg cgcagtttgt attcttcgcc
     8941 gcgctcttga aaaattttaa tcgcctccgc acgcggagtc atgattttga ccacatcata
     9001 gtcttgggca atcaattctt tcatacgcgc ttcaatggcg gcaacatctt ccggtgtaaa
     9061 cggtttttcc gtggcgatgt cgtaataaaa gccctcttca atgacgggac cgataaccat
     9121 ttttgcatta ggatagagtt gcttgacggc atgcccgaca agatgcgcgc aggaatggcg
     9181 gatgatttcg atgccttcct gatctttcgg agtaatgatt tgaacagcag aatcttcaac
     9241 aatcgggtcg cacgcatcga ccaatttgcc gtttaccctg cctgccaccg ccgccttcgc
     9301 caaaccgaca ccgatagacg cagcaatttg agccacggta acgggggatt cgtattggcg
     9361 gactgagccg tccggcaagg taatgttcaa catcaaaaag ctccaaaaca ttaaaaaata
     9421 acggcataaa tgccgttatg ggaatttggt aggcacgatt ggattcgaac caacgacccc
     9481 caccatgtca aggtggtgct ctaaccaact gagctacgtg ccttcaaaga atttttgcat
     9541 tttatcgggt cgttttaatt tttgcaagag gtatgtgcgt ttctttatgc aacattgcgg
     9601 caagatttgg ggatgtcgtc tgaaaaaccg tgggaaatca tttataatgc cgtcttttaa
     9661 aatatatttc gacccctgtc gcccaactga cattcaatct tatgcttaaa tttaccttac
     9721 acaaaaaaga cggtctcgcc cgacgcggca cgctggagtt gaaccacggc aaaattgaaa
     9781 cgccggtatt tatgcctgtc ggtacttacg gttcggttaa ggcgatgaac ccgcaaaacc
     9841 tgcacgatat caaggcacaa attattttgg gcaacactta tcatttgtgg ctgcgtccgg
     9901 gtttggaggt cgtcgaacag tttggagggc ttcacggctt tatcggctgg gacaaaccga
     9961 ttctgaccga ctcgggcggt tttcaggttt tttctttgtc ggatatgcgc aagctgacgg
    10021 aagaaggctg tacgttcaaa agcccgatca acggcgacaa gctgttttta tcgcctgaaa
    10081 tttccatgaa aatccaaacg gttttgaact cggacatcgc gatgcagctg gacgaatgca
    10141 cgccgggcga agcaacgcgc gaacaggcga aaaaatcgct gcaaatgagc ctgcgctggg
    10201 cggaacggag caaaaaagcc tttgaagatt tgaaaaaccc gaacgcgctg ttcggcatcg
    10261 tgcaaggcgc gatgtatgag gatttgcgcg aagaatcgct gcgcggtttg gaacagtttg
    10321 attttcccgg ccttgccgtc ggcggtttgt ccgtcggcga acccaagccc gaaatgtacc
    10381 gtatgctgcg cgccgtcggt ccgattctgc cggaacacaa gccgcattac ctgatgggcg
    10441 taggcacgcc ggaagacctc gtgtacggcg tggcacacgg catcgatatg ttcgactgcg
    10501 tgatgcccac ccgcaatgca cgcaacggct ggctgtttac ccgtttcggc gatttgaaaa
    10561 tcaaaaacgc caaacacaag ctcgacaagc gtccgataga cgaaagctgc acctgctacg
    10621 cctgccaaaa cttcagccgc gcctacctgc accatctgca ccgcgccggc gaaatcttgg
    10681 gcgcacagct caacaccatc cacaacctgc atttctacca agtcattatg gcggaaatgc
    10741 gcgatgccat agaacaaggc aaatttgccg actggcaggc gcgattccac gaaaaccgtg
    10801 ctcgcggcac agactgactc ccggcaatgc cgtctgaagc cgcttcagac ggcattttgc
    10861 cgctttaccc tacccgcact atttttcaga aaccgatcat gcttccattc tttccagaac
    10921 cctccctttc ctatacgcaa caaaaccgta ccgccgtttt gctgttgaac ctcggcacgc
    10981 ccgacgcgcc gaccgcgcag gcggtacgac cttatctgaa gtcctttctg accgaccggc
    11041 gcgtcgtcga actgcccaaa tggctgtggt atcccattct gcatggtctg gtactgacgt
    11101 tgcgccccaa aaaaagcgcg cacgcctatg aaaaaatctg gtttaaggaa ggttcgcccc
    11161 tagaagttta cacagcacgc caagctgccg cgcttgccga acgtatgccc gacctcatcg
    11221 tccgccacgc catgacctac ggcaatcctt cggtggcaga cgtattgtcg gaactgaagg
    11281 cgcaaggcgc gggcagactg ctggtcatcc cgatgtaccc gcaatatgcc gcttccagca
    11341 gcggagcggc agtagataag gtgtgcgaac aattactgct gcaacggaac caaatgagcg
    11401 tccgcaccgt ttcgcgcttt tatgacgata ccggctacat tgatgcgatg aaaaaccaca
    11461 tcctccgata ttgggcggaa cacggacgtg ggaaaaaact gatgttgagt tttcacggcg
    11521 taccgcagaa gcactacgac ctcggcgacc cctatcccga cgagtgccgc cacaccgcca
    11581 aactgcttgc cgaagcactg gaactgaccg aagaccaata tgtcgtatcg ttccaaagcc
    11641 aattcggcag ggcaaaatgg gttacaccga gcacgcagga tttgttcggc aaactgccca
    11701 aacagggggt aaccgagctg gacgtattct gcccgggctt tttggcagac tgtttggaaa
    11761 ccatggaaga aatcgccctg atggggcgtg aacagtttta cgaagcaggc ggcaaaagct
    11821 accgctacat cccctgcctc aacgacaacc ccgactggat agacgcactc gtcgcacttg
    11881 ccgaaggaaa ccttggcggc tggcgttgaa actgtttgac ccatgtctaa aatgccgtct
    11941 gaacgccgtg ttcagacggc attttcccgc tactttagcg tatgatggca tctatgccgg
    12001 acagcacatc aagtgcaacc ttaccaccga ttcggcaaaa aaacctttca cccgctacgc
    12061 aaaatcaaca acaaggtttc aaacattaag gaaacaccaa tgaacacaac ccgactgccg
    12121 accgccctcg tcttgggctg cctctgcgcc gccgcttctg ccgccgacaa cagcatcatg
    12181 acaaaagggc aaaaagtgta cgaatccaac tgcgtcgcct gccacggcaa aaagggcgaa
    12241 ggccgcggaa ccatgtttcc gccgctctac cgctccgact tcatcatgaa aaaaccgcag
    12301 gtgctgctgc acagcatggt caaaggcatc aacggtacaa tcaaagtcaa cggcaaaacc
    12361 tacaacggat tcatgcccgc aaccgccatc agcgatgcgg acattgccgc cgtcgccact
    12421 tatatcatga acgcctttga caacggcggc ggaagcgtta ccgaaaaaga cgtaaaacag
    12481 gcaaaaggca aaaaaaacta aacagacaaa atgccgtctg aaaccggcaa tccggcttca
    12541 gacggcattc aaatcaaacc tttctaacgc ggtactttca gccccataga cccgatattg
    12601 ccgccaccgt caacctcctt tgcggtcagc ataaagccgc cgatgtcaat acggccgcac
    12661 tcgaccggct cctgactgtc ggaacgctta tcgaaaagct cggcaaggct caaaccctct
    12721 tcgcccgctt ccagcctcaa accgtaagca agtgccaaat cacccgaacg tgctgccggc
    12781 aaaacgacaa actcaccgaa gaaatcaaaa tactcacgga cgctaatacc cgtctcggta
    12841 aagtatttcg ccattttata gtgaactaaa tt
//
LOCUS       DAWFHY010000060        12515 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.060, whole genome shotgun sequence.
ACCESSION   DAWFHY010000060 DAWFHY010000000
VERSION     DAWFHY010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.060"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            141..323
                     /locus_tag="WGQ68_001465"
     CDS             141..323
                     /locus_tag="WGQ68_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715245.1"
                     /translation="MPTPPAALMVAPVRPNPPKDGKTATLLEHAAEFGGYVAELENQN
                     QAWRDWAGNHSRKVGN"
     gene            complement(368..538)
                     /locus_tag="WGQ68_001466"
     CDS             complement(368..538)
                     /locus_tag="WGQ68_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEZ0715246.1"
                     /translation="MYFEIYKDAKGEYRWRLKAANHEIIAQGEGYTSKQNCQHAVDLL
                     KSTTAATPVKEV"
     gene            551..769
                     /locus_tag="WGQ68_001467"
     CDS             551..769
                     /locus_tag="WGQ68_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715247.1"
                     /translation="MNEYTFSYRFNGKSWSLSIWADNPEEARAKFRAARENAHYDGEV
                     VAKVYTFVNISWVKKLYKRTKYLMGIKE"
     gene            766..1092
                     /locus_tag="WGQ68_001468"
     CDS             766..1092
                     /locus_tag="WGQ68_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715248.1"
                     /translation="MTYRELVERQLAVRHADLELGLSRAREQEPFVIHVSDLLDKAGI
                     EYAVRMDKDFQTTFCVEFSATAPADVIGILRKYYSVFSDGQKVEAASRHPECHAVRIV
                     FGDVPV"
     gene            1104..1370
                     /locus_tag="WGQ68_001469"
     CDS             1104..1370
                     /locus_tag="WGQ68_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715249.1"
                     /translation="MDFEFGFKTLWPIATAAFWFWVNGISGRLKEADKRIDDLKEELH
                     AVKLSYHTKQDAKADRDNIAASLGRIENKLEKVNEKLDRKADKS"
     gene            1367..1564
                     /locus_tag="WGQ68_001470"
     CDS             1367..1564
                     /locus_tag="WGQ68_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715250.1"
                     /translation="MSDPILEALARIENKTDQTLKNQKEMQAEIAQIRQDTKRTAITF
                     GALGGGVITVGWELLKAKMGL"
     gene            1567..2073
                     /locus_tag="WGQ68_001471"
     CDS             1567..2073
                     /locus_tag="WGQ68_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1804 family protein"
                     /protein_id="HEZ0715251.1"
                     /translation="MAHPQEIREKLRRLYVSGEQTLETAALMCEIPQATARAWKRSDK
                     EKGDDWDKMRAAYTLAGGGIEDLSRAMLAGFMVQYNSTMTMLQDSSTEDLPPSDRAKL
                     LASLADAFTKTVSANARVMPETSKLATALELIEFLMAFVQEKHPKHLPAFVEVLEPFG
                     VEVEKKFG"
     gene            complement(2106..2582)
                     /locus_tag="WGQ68_001472"
     CDS             complement(2106..2582)
                     /locus_tag="WGQ68_001472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715252.1"
                     /translation="MTGFISNAFIGLKEAGSLLKGFNQLKTEAEVAQKTIELNQIIAG
                     IQTDLFEAQSSYTAAISRIDELEKELVAVKNWSTEKQRYQLYELVPGSFVYRLKAEMA
                     NSEPIHDICPQCYEQGVKSILQFNGYEGAFHKYSCSRCQTIILGERLKSDIEVFSG"
     gene            2594..4216
                     /gene="terL"
                     /locus_tag="WGQ68_001473"
     CDS             2594..4216
                     /gene="terL"
                     /locus_tag="WGQ68_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit"
                     /protein_id="HEZ0715253.1"
                     /translation="MKTKEFLKSLAELAASLRQVIEAEVDGFDASPKAIAARRAKVFD
                     PVGGYEYFVNTYFPHYIRSPEKSELHEFLFSRLPEIIHSPKGENEAVGAPRGEGKSTQ
                     VTQLFTLWCIVTGQKHYAVIVMDSIDQAYPMLEAIKAELEFNPRLKTDFPEVCGQGRV
                     WQAGTIVTANDVKVQVAGSGKKLRGLRHGPYRPDLTVLDDIENDEQVRNPEQRDKLNA
                     WLTKTVLPLGGVGQKYDVIYIGTILHYDSVLNRTLNNPFWHGIKFKAMKRWPDRMDLW
                     DRWEELFRNDGETVAEAFYLANKDEMERGAQTSWAARGVLALMKIRARDGHATFDSEY
                     QNDPVSGEDAPFAKSMKFWNDLPSDLVYFGALDPSLGKAGASRDPSAIIIGGYQRETG
                     KLYVVEAQIKKRLPDLIIEDVIRLHRQYRCKLWFVETVQFQEFLKDELVKRSAARGIP
                     VPARAVKPVSDKLLRIETLQPHMANGLILLNESQQTLIQQFRHFPKADHDDGPDAVHM
                     LWSGAVANCVPIEWQSPTDNDFDDEIKSKWSR"
     gene            4216..5784
                     /locus_tag="WGQ68_001474"
     CDS             4216..5784
                     /locus_tag="WGQ68_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF935 domain-containing protein"
                     /protein_id="HEZ0715254.1"
                     /translation="MAKKNNKTKIQKPEAALQADVAQITATGRVIAEHPSNFITPQKM
                     RALFEDAESGDIRAQHELFADIEERDSDIAANMGTRKRALLTLNWRVAPPRNATPEEE
                     KLSDQAYEMMDSLPTLEDLIMDLMDAVGHGFSALEVEWVFSDGLYLPRNFIHRPQSWF
                     KWDKDNGLLLRTRENPEGEALWPLGWVVHTQKSRSVQQARNGLFRTLSWLYMFKHYAV
                     HDFAEFLELYGMPIRIGKYGAGATKEEKNTLLRAVAEIGHNAAGIMPEGMEIELHNAA
                     SGTMATSNPFLQMADWCEKSAARLILGQTLTSGADGKSSTNALGNIHNEVRRDLLVSD
                     AKQVAQTITSQIIGPFLQINYPNADPNRVPKFEFDTREPKDIAVFADAIPKLVDVGVQ
                     IPESWVRDKLVIPDVQEGEAVLVRQVPDNPINRTALAALSAHTVPSKATGRHQEILDG
                     ALDDALVEPDFNSQLNPMVRQAVAALNACNSYEEADAALNALYPNLDNAKLRTYMQQA
                     LFISDILGQDHARA"
     gene            5771..7066
                     /locus_tag="WGQ68_001475"
     CDS             5771..7066
                     /locus_tag="WGQ68_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEZ0715255.1"
                     /translation="MPAPDLGFALSLPPKKAIEWLESKKVTAESYRNLTASEIAKVYT
                     IARMTDLDMLNDIKTSMVESAKSGQSFDDWRKGILNLLGNKGWLHPNGHNGKDIIDPA
                     TGEVFGSPRRLETIYRTNMQTAYNAGQYQGYMANIDARPYWMYDAVGDSRTRPAHSAI
                     DGLVYRYDDPFWATFYPPNGYNCRCSVIALSERDVERQGRIVGQSTADNLVETHKIYN
                     KKGDSYPTTAYKAPDGSLYTADKGFGYNAGRMNYRPDLDKYDRALAHQFAKAEMGGAD
                     FKTSFKQLEKEFYEVKQRLDIDGKPDKEQKIKIRNALSRQLKFAAGVLSKEMQELAGM
                     TRATVWLSDDTLVKQVDSREGQNFDDSYYAFLPDMLQNPEHVIRDNRELIFTARYKGS
                     ALWAVLKYIKEVDEIYLQSYRISNDKEIAKFMAKKKVLK"
     gene            7176..7592
                     /locus_tag="WGQ68_001476"
     CDS             7176..7592
                     /locus_tag="WGQ68_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HEZ0715256.1"
                     /translation="MIDVKIDNIFVVLNQIERLGNGIENRYLLMRRLSETMHTAVKLN
                     FRYAGRPKWLGLKYRDGKLLSDSGRLKDSFSTLSDNDTALVGTNIVYAAIHNFGGMAG
                     RNRKVRIPQREFLTLTDDDKQALMDDVQDYFSGLIP"
     gene            7823..8884
                     /locus_tag="WGQ68_001477"
     CDS             7823..8884
                     /locus_tag="WGQ68_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715257.1"
                     /translation="MSKNAQKTLLAVCSFEVQPKDGRIQLLPYGEFRAVDGRPTDVPA
                     WYLTEENGHDVALLANSSRNQLVVDYEHQTLYKEKNGQPAPAAGWMRWLEFTPKGMFA
                     EVEWTDKAAAAIAAKEYRYISAVFSYDTKGYVSKIFHAALTNFPALDGMDEVLAAASA
                     QILKPETEQNPMKELLQQLFDLPDAGEEELKAALSALVEAKPKDVALSADVFAQLAEK
                     DSRIAALTAQTAKPDLTKYAPISVVQELQSKVAALTAKQEADKGNELITAALTSGKLL
                     PAQKEWAKGVLKQPGGLAFLTGFIENAQPVAALAGSQTGGKAPDERVAALTAEEAAAA
                     KMLGMSGEEFVKIKESEGK"
     gene            8934..9272
                     /locus_tag="WGQ68_001478"
                     /pseudo
     CDS             8934..9272
                     /locus_tag="WGQ68_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9346..9957
                     /locus_tag="WGQ68_001479"
     CDS             9346..9957
                     /locus_tag="WGQ68_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HEZ0715258.1"
                     /translation="MAAPAGVATECRLTVQGGTDRESDASLLARLLEIIRRPPAGGNR
                     YDYKNWALSVDGVTSAYVYPLRRGLGTVDIAITSADGVSSEETVRRVQAYIDEMRPVT
                     AKNALVLKPTVTAVPVTVQVKLDGIDLDEAKRRIRTALKEYFDTLIPGDGLTVSQIEA
                     AISNVDGVIDRRLTAPTANRAADTVNRIEWFKAGAINVTEMPS"
     gene            9954..10514
                     /locus_tag="WGQ68_001480"
     CDS             9954..10514
                     /locus_tag="WGQ68_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HEZ0715259.1"
                     /translation="MSYQDILRGLLPPVSYARNAPRVRAQAEIDGAALDAVAESAQSV
                     ADAVDPRSAGQMLADWERVLGLDGTGKNRQHRVLAVMAKLNETGGLSIPYFVRLAEAA
                     GYQIQIDEPQPFRAGVNRAGDRLAPQEIMWVWHVNVRGGNNRITRFRAGISAAGDRLT
                     DYSDAVIESLFNRLKPAHTAIRFTYR"
     gene            10525..12501
                     /locus_tag="WGQ68_001481"
     CDS             10525..12501
                     /locus_tag="WGQ68_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HEZ0715260.1"
                     /translation="MHPIETPDKTFHDGDGVSELDTILPAWWLNQVQSELLAVLTAAG
                     IQPDKSQPNQLLAALNRLAVVTTGNQEIGGQKTFTAQTQFPSGIHLSANQTNWNGGHK
                     AYIGADADNAHIVFGDDTLRLHSANNRISYNNHDIFHKANKPRFAEDIEGKPNTLSGY
                     GIGNFKVETFRGDLNTLKTDGIYSLPTAVGSSNLPVENTACHIQVIAGTQPGWCRQLG
                     YPAYTSDVYERHQTSSANDNWSAWKKLNSDGIPVGAIVSFPKAVRNPAGYLRADGTTF
                     AQNTFPDLYRALGNSNRLPDLSRTDIGITAWFPSDQIPTGWLAFDDIRTRVTETAYPE
                     LYRLLTGKYGSIQNVPQAEDRFIRNAGNSLAVGTKQEDEIKRHVHKVFSHWTNHTDAA
                     ALGYEDRNERQRSALVSTWTDENLNDNGFLTPRSDSKMATGGDENRPKALVLKLCIKA
                     ADTLGEAVFWIKSHGETINAGALDAGTLAQNLQDKADRAHTHTAAQIQGLDEKISTAV
                     AAQFTRQTIGGVDIVRFPDGTMIQTGSYRFTRSGGPIENEVVFPVAFADGNVKCFVSE
                     RHSGRVTGDRRQHNWLFIRAKNHAAAIITNWYEGSCDWMAIGKAASGNAASSPIGPEI
                     PETNEEPQRESGRTSTGPRNRRRRRDGLLEALQD"
BASE COUNT         3206 a         3292 c         3443 g         2574 t
ORIGIN      
        1 taaaaaggaa atcgaaaatg tccttactca agaccgtaaa aatgcaggcg gcggttgtat
       61 tgacggcttt ggccatcacg gcttgcagct ctacaagcgc gccctcggct acggaaatta
      121 aggttgtcga aaaggcggtc atgccgacac cgcctgccgc attgatggtc gcgccggtac
      181 gcccaaatcc gccgaaagac ggcaagacgg ccacgctgtt ggaacacgcc gccgagtttg
      241 gcggctatgt ggcggagttg gaaaatcaaa atcaggcttg gcgcgactgg gcgggcaatc
      301 actcccgcaa agtcggaaac tgacaaaaaa gcccgcgtag ggcgcgggct gagggtgaaa
      361 gcggatttta tacctctttt acaggggtag cggcggtagt gcttttcagc aaatcgactg
      421 cgtgctgaca gttttgcttg ctggtgtagc cttcgccctg agcgatgatt tcatggttgg
      481 ctgctttcaa acgccaacgg tattcgcctt ttgcgtcttt atagatttca aaatacataa
      541 ggtttctcct atgaatgagt acacgttttc ttaccgcttt aacggcaagt cctggtcatt
      601 gagcatttgg gcggacaacc ctgaagaagc cagggcgaaa tttcgggctg cacgagaaaa
      661 tgcgcactat gacggcgaag ttgtagcaaa ggtttataca tttgtaaata tttcgtgggt
      721 taagaaattg tacaagcgga caaaatattt aatgggtatc aaagaatgac ctaccgtgaa
      781 ttagttgaac gtcagttggc tgtgcgccat gccgatttgg aattgggctt aagccgcgca
      841 cgcgagcaag agccgtttgt cattcatgtt tccgatctgt tggataaggc aggcattgag
      901 tacgcggtac gcatggataa ggattttcag acgacctttt gtgtggagtt ttccgcgacc
      961 gcccctgctg atgtgattgg tattttgcgg aaatattact cagtcttttc agacggccaa
     1021 aaggtcgagg cggcgagtcg tcatcccgaa tgccacgcgg tccgtatcgt atttggcgat
     1081 gtgccggttt aaaggggttt taaatggact ttgaatttgg ttttaaaacc ctgtggccga
     1141 ttgcgacggc ggcattttgg ttttgggtca atggcatttc aggccgtctg aaagaggcgg
     1201 acaagcgtat cgatgacctt aaagaggagc tgcacgcggt caagctctct tatcacacca
     1261 agcaagatgc caaggcagac cgcgacaata tcgcagcttc tttgggacgc atcgaaaaca
     1321 agttggaaaa agtaaacgaa aaactggaca ggaaagcgga caaatcatga gcgacccgat
     1381 tttggaagcc ttggcgcgta ttgaaaacaa gactgatcaa acgctgaaaa atcagaagga
     1441 aatgcaggcg gaaattgcgc aaatccgcca agacacgaaa cgcacggcca ttacattcgg
     1501 cgcactgggc ggcggcgtga ttacggtcgg ctgggaattg cttaaagcga aaatgggact
     1561 gtaattatgg ctcacccgca agaaatccgt gaaaagttac gccggctcta tgtgagcggc
     1621 gagcaaactt tggaaacggc ggccttgatg tgcgaaatcc cgcaggccac tgcgcgtgcg
     1681 tggaaacgtt cggataagga aaaaggcgac gactgggata agatgcgcgc cgcttacact
     1741 ttggccggcg gcggtattga ggacttgagc cgtgcgatgt tggccggttt tatggtgcag
     1801 tacaacagca cgatgacgat gctgcaggat tcgagtaccg aagatttgcc gccgtccgac
     1861 cgtgcgaagc tgttggccag cctggccgat gcgtttacga aaaccgtatc cgccaatgcc
     1921 cgtgtgatgc cggaaacgtc aaaactggcg acggctttgg aattgattga gttcttgatg
     1981 gcgtttgtgc aagaaaaaca tcccaaacat ttgcctgcct ttgtggaggt attggagccg
     2041 tttggggtgg aagtggagaa gaagtttggt taatagccgt ctatcaatct cccataacgt
     2101 gatcctcatc ctgaaaatac ttcaatatct gactttaaac gctctcccaa tattatggtt
     2161 tggcaacggg aacaggaata tttatgaaaa gcaccttcat acccattgaa ttgaagaatc
     2221 gatttgacac cctgctcgta acattgggga caaatatcat gaattggctc gctgttcgcc
     2281 atctccgctt tcaatcgata gacgaaagaa ccaggaacaa gttcatacaa ttggtagcgt
     2341 tgcttctctg tagaccaatt ttttactgcc accagttctt tttccaactc atctatacgg
     2401 ctaattgccg ccgtataaga agattgggct tcaaataaat cagtttgaat accagcaatg
     2461 atttgattaa gttcgatggt tttttgcgct acctctgcct ctgttttgag ctggttaaac
     2521 ccttttaaaa gtgatcctgc ttcttttagg cctataaatg cgttagaaat gaatccagtc
     2581 attataaata ctcatgaaaa caaaagaatt cctcaaatcc cttgccgaac tggccgccag
     2641 cctgcgccaa gtcatcgaag cggaagtgga cggcttcgat gcgtcgccca aggctattgc
     2701 tgcacgccgt gccaaggtgt ttgacccggt aggcggttac gagtatttcg tcaacaccta
     2761 ctttccgcat tatatccgct cccctgaaaa atccgaactg catgagttct tattcagccg
     2821 tctgccggag attatccact cccccaaagg ggaaaatgag gcggtaggtg cgccgcgtgg
     2881 cgagggtaag tcgacgcagg ttactcagtt gtttacgctg tggtgtattg tgacaggcca
     2941 aaaacattat gccgttattg tgatggacag tattgaccag gcatacccga tgctcgaagc
     3001 catcaaggcg gaattggaat ttaatccgcg cttgaaaacc gactttccgg aagtatgcgg
     3061 gcagggccgt gtatggcagg ccggtacgat tgtgacagcc aatgacgtta aagtccaagt
     3121 ggccggtagc ggtaaaaagc tgcgcggttt gcgtcacggc ccttaccgtc ctgacttaac
     3181 tgttttggac gatattgaga atgacgagca agtccgcaac cccgaacagc gcgacaagct
     3241 caatgcgtgg ctgactaaga ctgtattgcc tttgggcggt gtcggccaga aatacgatgt
     3301 gatatatatc ggcacgattt tgcattacga cagcgtactt aaccgcactt tgaataaccc
     3361 gttttggcac ggtattaagt ttaaggcgat gaaacgctgg cccgaccgca tggatttgtg
     3421 ggacagatgg gaggaacttt tccgaaacga cggcgagact gtggccgagg cgttttatct
     3481 cgccaataaa gacgagatgg agcgcggtgc gcaaacaagc tgggcggctc gcggcgtact
     3541 cgcgctgatg aaaatccgtg cgcgtgacgg tcatgcgaca tttgacagcg aatatcaaaa
     3601 cgacccggtc agcggcgaag atgcgccgtt tgccaagtcg atgaagtttt ggaacgacct
     3661 gccgtccgat ttggtgtatt tcggtgcgct cgacccgtcg ctgggtaaag cgggggcgag
     3721 ccgtgacccg tcggcgatta tcattggcgg ttatcaacgt gaaaccggca aactgtatgt
     3781 cgtggaagct cagattaaaa aacgtctccc tgatttgatt attgaggacg ttatcagatt
     3841 gcaccgtcaa tatcgttgca aactgtggtt tgttgagacg gttcagtttc aggaattttt
     3901 gaaagatgag ctggtcaagc gcagcgcggc gcgtggaata cctgtcccgg cgcgggcggt
     3961 caagccggta tcggacaagc tcttgcggat tgagacttta cagcctcaca tggcgaacgg
     4021 tttgattctg ttgaatgaga gccaacagac gctgatacag cagttccgcc attttccaaa
     4081 ggctgatcat gatgatggtc ctgatgcagt gcatatgctc tggtcggggg cggtggccaa
     4141 ttgtgtgccg atagaatggc aaagccctac cgataacgat tttgatgacg agataaaaag
     4201 taaatggagc cgataatggc aaaaaagaac aataaaacta aaatccaaaa gcccgaagct
     4261 gcattgcagg cggacgtggc tcaaattacg gcgaccggtc gggttatcgc cgagcatccg
     4321 tccaatttta ttacaccgca aaagatgcgg gccctcttcg aggatgcgga aagcggcgac
     4381 atccgcgccc aacacgagct tttcgcggac attgaggagc gcgacagcga catcgcggca
     4441 aatatgggga cgcgcaaacg cgcgctgctg acgctcaact ggcgcgtcgc cccgccgcga
     4501 aatgcgacgc ccgaagaaga aaagctgtcc gaccaagcct acgaaatgat ggacagcctg
     4561 cctaccctcg aagacctgat tatggatttg atggacgcgg tagggcacgg attttctgcg
     4621 ttggaggtcg agtgggtatt ttcagacggc ctttacctac cccgaaactt tatccaccgc
     4681 ccgcaaagct ggttcaaatg ggacaaagac aacgggctgc tgctgcgtac ccgcgaaaat
     4741 ccggaaggcg aagcgttgtg gccgctgggc tgggtcgttc atacccaaaa atcgcgcagc
     4801 gtccagcagg cgcgcaacgg gcttttccgc acgctttcct ggctgtatat gttcaaacac
     4861 tacgccgtcc acgattttgc cgagtttttg gagctgtacg gcatgcccat ccgtatcggc
     4921 aaatacggcg cgggcgcaac caaagaggaa aaaaacaccc tgcttcgagc ggtggcggaa
     4981 atcggtcaca acgcggcagg catcatgcca gaaggtatgg aaatcgagct gcacaacgct
     5041 gcaagcggta cgatggcaac cagcaatccg tttttgcaga tggccgactg gtgcgaaaaa
     5101 tcggcggcgc ggctgatttt ggggcaaacg ctaaccagcg gtgcggacgg aaaatccagc
     5161 accaacgcgc tgggcaatat ccacaacgag gtacgccgcg atttgctggt gtcggacgca
     5221 aaacaggtgg cgcaaaccat cacaagccaa atcatcggac cgttcctgca aatcaactat
     5281 cccaatgccg acccaaaccg cgtgccgaaa tttgaatttg acacgcgcga gccgaaagac
     5341 atcgcggtct ttgccgacgc tatcccgaaa ctggtggatg tcggcgtaca aatccccgaa
     5401 agctgggtgc gcgacaaact ggtcattcca gatgtgcagg agggtgaggc tgtgttggtg
     5461 cggcaggtac cggacaatcc gataaacaga actgcattgg cggctttatc cgcccacacc
     5521 gtaccatcta aggctacggg caggcatcag gaaatattgg acggcgcgtt ggatgacgcg
     5581 ctggttgagc ccgatttcaa ttctcagctc aacccgatgg tgcgtcaggc ggttgccgca
     5641 cttaatgctt gcaacagcta cgaggaggca gatgccgcac tgaatgcgct ttatccgaat
     5701 ttggacaacg cgaaactgcg tacctatatg cagcaggcct tgtttatcag cgatattttg
     5761 ggacaagacc atgcccgcgc ctgatttggg atttgcctta agtctgccgc caaaaaaggc
     5821 aatcgagtgg ctggaaagta aaaaggttac ggcggagagc taccgcaatc tgacagcctc
     5881 cgaaattgcc aaagtctata cgattgcccg catgaccgac ttggatatgc tcaacgacat
     5941 caaaacttcg atggttgaat cggcaaaaag tggacagtcg tttgacgatt ggcgaaaagg
     6001 tatcttgaat ctgctcggca acaagggctg gctgcatccg aacgggcata acggtaagga
     6061 tatcatcgac ccagccaccg gcgaggtatt cggttcgccg cggaggttgg agacgattta
     6121 ccgtaccaac atgcaaactg cctacaacgc cggtcaatat caaggatata tggcaaatat
     6181 tgatgcacgg ccttattgga tgtatgacgc ggtaggcgac agccgcaccc gtccggcgca
     6241 ttcggcaata gacgggctgg tgtaccgcta cgacgacccg ttttgggcaa cgttttaccc
     6301 gcccaacggc tacaactgcc gttgctcggt catcgcgctg tcggagcggg atgtggaacg
     6361 ccaagggcgg attgttgggc aaagcacggc ggacaatctg gtcgagaccc ataaaatcta
     6421 caacaaaaaa ggcgacagct acccgacgac tgcctacaaa gccccggacg gcagcctcta
     6481 cacagctgac aaaggcttcg gctacaacgc cgggcgaatg aactaccgcc ccgatttaga
     6541 caagtacgac cgtgcgttgg cgcatcaatt tgccaaagcg gaaatgggtg gtgcggattt
     6601 taaaaccagc tttaaacagc ttgaaaaaga gttttatgaa gtcaagcaac gtttggatat
     6661 tgatggcaag cccgataaag agcagaaaat caaaatccga aatgcgctat caagacagct
     6721 taaatttgct gcgggtgtat tgagcaagga aatgcaagaa ctggcaggta tgactcgagc
     6781 gacggtgtgg ctgtctgatg atacattggt caaacaggtg gacagccgtg aggggcagaa
     6841 tttcgatgac tcctactatg cttttttgcc ggatatgctg caaaaccctg aacatgtcat
     6901 ccgcgacaat cgtgaattga ttttcacagc tcgctataaa ggctcggcat tgtgggcagt
     6961 tttaaaatat attaaggagg tggatgagat ttatctacag tcgtaccgaa tcagtaacga
     7021 caaagagatt gccaaattta tggcgaagaa gaaagtattg aaatagacgt tgggcaaggc
     7081 tcgaaatcac ttgcacacgc tctcggacgc cctaacgggc aggctgcggt atcgaggtta
     7141 tcaccgcttt tccaacgtct gtacggaata ataccatgat tgatgtcaaa atagacaata
     7201 tctttgtcgt cctaaaccaa atcgagcggc ttggcaacgg gatcgaaaac cgctacctgc
     7261 tgatgcgccg actgtccgaa accatgcaca cggcggtcaa gctcaatttc cgctacgcag
     7321 gccgtccgaa atggttgggg ctaaaatacc gcgacggcaa gctgctttcg gattcgggtc
     7381 gtctgaaaga cagtttttcc acactgtcag acaacgatac cgcccttgtc ggtacgaata
     7441 tcgtctatgc cgccatccac aacttcggcg gtatggcggg gcgcaaccgc aaagttcgga
     7501 ttccgcaacg ggaatttttg acgctgacgg acgacgacaa acaggctttg atggacgatg
     7561 tgcaggatta tttttcgggt ctgataccgt gaatttataa aaccctcaaa aacgcgcttt
     7621 ttagcgcgtt tttttatgcg ggtaatacaa acccctgccc aagatataaa aatcaatcct
     7681 agactcttct aaaaagcccc tgaaaacgat taattgtgta tcgcgcggac aggttttaaa
     7741 aaaatggcgg gagggtttga agcacgccta ctctttgttg ttttttcaaa taggcaaaat
     7801 gaccgtattg agagaggtac acatgtccaa aaatgcacaa aaaaccctac ttgccgtgtg
     7861 cagtttcgag gtgcagccaa aagacgggcg aatccaactg ctgccatatg gcgaatttcg
     7921 cgcagtagac ggtcgtccga ctgatgtccc tgcgtggtat ctgaccgaag aaaacggtca
     7981 tgatgtcgcg ttgttggcca acagctcgcg caatcagttg gttgtcgatt atgaacacca
     8041 gacgctctac aaagagaaaa acggacaacc tgcacctgcc gccggttgga tgcgttggct
     8101 ggagttcacg cctaaaggca tgtttgccga agtggagtgg acggacaagg cggctgcggc
     8161 aattgccgca aaagagtatc gctacatctc tgctgtgttt tcctatgaca caaagggata
     8221 tgtaagcaaa atttttcacg ccgcgctgac aaatttcccc gcgttggacg gtatggacga
     8281 ggtgctggcg gcagcgtcgg cgcaaatttt aaaaccggaa acggagcaaa accctatgaa
     8341 agagttgtta cagcaactgt tcgacctgcc tgatgcgggc gaagaagaac tgaaggcggc
     8401 attgtccgcg ctcgtggaag ccaagccgaa agacgtggca ttgtctgccg acgtgttcgc
     8461 gcagctggcg gaaaaagaca gccgcatcgc ggcattgacg gcgcaaaccg ccaagcctga
     8521 tttgactaaa tacgcgccta tctcagtggt tcaagagctg caaagcaaag tcgccgcgct
     8581 gactgccaag caggaagcag acaaaggcaa cgaattgatt accgccgcgc tgacttcagg
     8641 caaattgctg cctgctcaga aggagtgggc aaaaggcgta ttgaaacagc cgggcggctt
     8701 ggcatttttg accggcttta ttgaaaacgc ccagccggtc gctgcactgg caggctcgca
     8761 aacgggcggc aaagcacccg acgaacgcgt cgccgcactg actgcggaag aggcagccgc
     8821 agcaaaaatg ctgggcatgt ccggcgaaga atttgtaaaa atcaaagaaa gcgaaggtaa
     8881 gtaatggaca aatcagcgat tttgaccgca atcacggcag cattccgcaa agaatttcaa
     8941 acgggggcct gcaaaaccgc ctgaaccggt taaccgccaa acaggtgcag ccggtagcgc
     9001 aggcggtgcg cctgttatcc acgtcatcgc tgttgtcggt ggcaacggca ttaatcgagg
     9061 cgcatggcga agagatgact gcgcccgatt tgattgaggt taaccgcgcc atgcgccgcc
     9121 gtatgcaggc cgagattgcc gccttgcggg cggtgcagac ggctgctgcc gagtctggtg
     9181 ggctgacgac caacgccgtg tataccgagg cttaccaaac ggcagaatcc ctgcgcgcgg
     9241 cggcaggccg tctgaatgcg ttggttgcgg cggcacggcg gaaataccgg caatcgccga
     9301 cgagccgggc gcggccgcca atgtggcgac ggcgaggcgc aactgatggc cgcccccgcc
     9361 ggtgtggcca ccgaatgccg ccttaccgta caaggcggca ccgaccgaga aagcgatgcc
     9421 tcactgctgg cgcgtctgtt ggaaatcatc cgccgaccgc ccgcaggcgg caaccgttac
     9481 gactataaaa actgggcgtt gagtgttgac ggcgtaacca gcgcatatgt ttatccgctg
     9541 cgccgcggct tgggtacggt ggatattgcc attacctccg ccgacggtgt gtcgtcggaa
     9601 gaaactgtgc gccgcgtaca ggcttatatc gacgagatgc gcccggtaac ggcaaaaaat
     9661 gcgctggtac tcaagccaac cgtaacggcg gtgcctgtta ccgtgcaagt caagctcgac
     9721 ggtatcgact tggacgaggc caagcgccgc atacggacgg ccctaaaaga atatttcgac
     9781 accctgatcc ccggcgacgg cctgactgtg tcgcaaatcg aggctgctat cagcaatgtg
     9841 gatggtgtga tcgaccgccg tctgactgcg ccgacggcca accgtgccgc cgatacggtt
     9901 aaccgcatcg agtggtttaa agcgggcgcg attaatgtaa cggagatgcc gtcatgagct
     9961 atcaagacat cttgcggggc ctgttgcccc ccgtgtcgta tgcccgcaat gccccgcgtg
    10021 tgcgggcgca ggcagaaata gacggcgcag cgctggatgc ggtggcggaa tcggctcaaa
    10081 gcgttgccga tgccgtcgac ccgcgcagcg ccggccaaat gctggccgat tgggagcgcg
    10141 tattaggttt ggacggtacg ggcaaaaacc gccagcaccg tgtgttggcc gtcatggcca
    10201 agctaaacga aacaggcggc ttgagtattc cttattttgt gcgtttggcc gaggcggcgg
    10261 gctatcaaat ccaaatcgac gaaccgcagc cgttccgcgc cggtgtaaac cgcgccggcg
    10321 accgtcttgc gccgcaggaa atcatgtggg tgtggcacgt taacgtgcgc ggcggcaaca
    10381 accgcattac ccgattccgc gccggtatct cggcggcggg cgacaggctg accgattaca
    10441 gcgatgccgt gatcgagagc ctgttcaacc gcctcaagcc cgcccacacc gctatccgat
    10501 ttacctaccg ataaggacta aaccatgcac cccatcgaaa cccccgacaa gaccttccac
    10561 gacggcgacg gcgtgtccga attggacacc atcctgcccg cgtggtggct caaccaagtg
    10621 caatccgagc tgctggccgt gctgactgcg gccggtatcc agccggataa gtcgcagcct
    10681 aatcaattac tggcagcact gaatagactg gctgtagtta ccaccggcaa ccaagaaatc
    10741 ggcggccaaa aaacctttac tgcccaaacc caattcccaa gcggcatcca tttatccgcc
    10801 aaccagacga actggaacgg cggccacaaa gcctacatcg gcgcggatgc cgacaacgct
    10861 cacatcgtct tcggcgacga caccctccgc ctgcacagcg caaacaaccg catttcctac
    10921 aacaaccacg acatcttcca caaagccaac aaaccgcgtt ttgccgaaga catcgaaggc
    10981 aaaccgaaca cactgtccgg atacggcatc ggcaatttca aagtcgaaac attccggggc
    11041 gatttgaaca ccctcaaaac agacggcatc tattccctgc cgacggcggt cggcagctcc
    11101 aacctgcccg ttgaaaacac tgcctgccat atccaagtca tcgccggtac gcaacccggc
    11161 tggtgcaggc agttgggtta tcccgcctac acgtccgacg tgtacgaacg ccaccaaacg
    11221 agcagcgcaa acgacaactg gtccgcatgg aaaaaactca attcagacgg catccccgtc
    11281 ggcgcgatcg tatcctttcc caaagccgtc cgaaaccccg caggctattt gcgggcagac
    11341 ggcacaacct ttgcgcaaaa caccttcccc gacctttacc gcgccttggg caacagcaac
    11401 cgcctgcccg atttaagccg taccgacatc ggcatcaccg cgtggtttcc gtccgaccaa
    11461 atcccgaccg gctggctggc gtttgacgac atccgcacgc gcgtaaccga aaccgcttat
    11521 cccgagctgt accgtctgct gaccggaaaa tacggcagca tccaaaacgt cccgcaggcg
    11581 gaagaccgct ttatccgcaa cgcgggcaac agcttggcag tcggaacgaa gcaggaagac
    11641 gaaatcaaac ggcacgtcca caaagtattt tcacactgga caaaccacac agacgcggca
    11701 gccctcggtt acgaagaccg caacgaaagg cagagaagcg cgctcgtatc gacttggacg
    11761 gacgaaaatt taaacgacaa cggcttttta accccgcgct cggacagcaa aatggcgaca
    11821 ggcggcgacg aaaaccgccc caaagccctg gttttaaaac tgtgcatcaa agccgccgac
    11881 accttgggcg aagccgtgtt ttggataaag tcccacggcg aaaccatcaa cgcaggcgcg
    11941 ctggacgcgg gcacgctggc gcaaaacctc caagacaaag ccgaccgcgc ccacacccac
    12001 accgccgccc aaatccaagg gctggacgaa aaaatcagca ccgccgttgc cgcgcaattc
    12061 acacgccaaa ccatcggcgg cgtggatatt gtcagattcc ccgacggcac aatgatacag
    12121 accggcagtt acaggtttac aagaagcggc ggccccatcg aaaacgaagt cgtcttcccc
    12181 gtcgcctttg ccgacggcaa cgtcaaatgc ttcgtatccg aacgccattc gggacgcgtt
    12241 accggcgatc gaaggcagca caactggctg tttatccgcg caaaaaacca cgccgccgcc
    12301 attattacca actggtacga aggcagttgc gactggatgg ccatcggcaa agccgcctcg
    12361 ggaaacgccg ccagctcccc gataggcccc gaaatacctg aaaccaacga agaaccgcaa
    12421 agagagagtg gaagaacatc aaccggaccc cgaaaccgcc gccgccgccg agacggcttg
    12481 ctcgaggcac tgcaagacta gcgggctgta gagac
//
LOCUS       DAWFHY010000061        12113 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.061, whole genome shotgun sequence.
ACCESSION   DAWFHY010000061 DAWFHY010000000
VERSION     DAWFHY010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12113)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12113)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12113
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.061"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            202..1878
                     /gene="ettA"
                     /locus_tag="WGQ68_001482"
     CDS             202..1878
                     /gene="ettA"
                     /locus_tag="WGQ68_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEZ0715261.1"
                     /translation="MSQQYVYSMLRVSKVVPPQKTIIKDISLSFFPGAKIGLLGLNGA
                     GKSTVLRIMAGVDKEFEGEAVPMGGIKIGYLPQEPELDPEKTVREEVESGLGEVAAAQ
                     KRLEEVYAEYANPDADFDALAEEQGRLEAIIAAGSSTGGGAEHELEIAADALRLPEWD
                     AKIDNLSGGEKRRVALCKLLLSKPDMLLLDEPTNHLDAESVEWLEQFLVRFPGTVVAV
                     THDRYFLDNAAEWILELDRGHGIPWKGNYSSWLEQKEKRLENEAKSEAARVKAMKQEL
                     EWVRQNAKGRQAKSKARLARFEEMSNYEYQKRNETQEIFIPVAERLGNEVIEFVNVSK
                     SFGDKVLIDDLSFKVPAGAIVGIIGPNGAGKSTLFKMIAGKEQPDSGEVKIGQTVKMS
                     LIDQSREGLQNDKTVFDNIAEGRDILQVGQFEIPARQYLGRFNFKGSDQSKIAGNLSG
                     GERGRLHLAKTLLGGGNVLLLDEPSNDLDVETLRALEDALLEFAGSVMVISHDRWFLD
                     RIATHILACEGDSKWVFFDGNYQEYEADKKRRLGEEGTKPKRIKYKPVTR"
     gene            complement(1938..2423)
                     /locus_tag="WGQ68_001483"
     CDS             complement(1938..2423)
                     /locus_tag="WGQ68_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEZ0715262.1"
                     /translation="MADFKPDFAWLLKRPLCFLAFGFGSGLAPFAPGTFGTLAALPLA
                     FVLILLGIDGLLLAFLCIVLFMWGIRICAYAERETGVSDHGGIVWDEIVAMLFVLAFV
                     PFRWTWWLAAFVLFRLFDALKPSPVGWFDKNLHGGLGIMADDMAAAVMTLIVLRIAML
                     F"
     gene            complement(2416..3372)
                     /gene="thiL"
                     /locus_tag="WGQ68_001484"
     CDS             complement(2416..3372)
                     /gene="thiL"
                     /locus_tag="WGQ68_001484"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEZ0715263.1"
                     /translation="MKEFDFIKRYLQTGTDKDVVLGIGDDAAIVRPREGFDLCFSADM
                     LLKDRHFFADVKPEDLAWKVLAVNISDMAAMGAIPRWVLLSAALPELDEVWLKRFCGS
                     FFGLAKKFGVTLIGGDTTKGDMAFNVTIIGELPKGRALRRDAAVAGDDIWVSGRVGMA
                     AAALNCRLKRCVLPDDVFAECEQKLLRPEPRVGLGLALLPFARAAQDVSDGIAQDLGH
                     ILTASGVGAEIWADSLPSLSVLKDILPRAQWLSYTLAGGDDYELVFTAPESCRSRVLD
                     AAERCGVPVTRIGKINGGCRLKVLDDGGRELELHSLGFDHFG"
     gene            complement(4223..4951)
                     /gene="rmpM"
                     /locus_tag="WGQ68_001485"
     CDS             complement(4223..4951)
                     /gene="rmpM"
                     /locus_tag="WGQ68_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding outer membrane protein
                     RmpM"
                     /protein_id="HEZ0715264.1"
                     /translation="MTKQLKLSALFVALLASGTAVAGEASVQGYTVSGQSNEIVRNNY
                     GECWKNAYFDKASQGRVECGDAVAAPEPEPEPEPAPAPVVVVEQAPQYVDETISLSAK
                     TLFGFDKDSLRAEAQDNLKVLAQRLGQTNIQSVRVEGHTDFMGSDKYNQALSERRAYV
                     VANNLVSNGVPVSRISAVGLGESQAQMTQVCEAEVAKLGAKVSKAKKREALIACIEPD
                     RRVDVKIRSIVTRQVVPAHNHHQH"
     gene            5315..6265
                     /locus_tag="WGQ68_001486"
     CDS             5315..6265
                     /locus_tag="WGQ68_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysB family HTH-type transcriptional regulator"
                     /protein_id="HEZ0715265.1"
                     /translation="MKLQQLKYALEVYRHNLNVSEAAEALFTSQPGISKQIKLLEEEI
                     GIQIFIRSGKRVVSVSQPGKVVLDIAERILRDVQNIKNIGSEFTGQDSGSLTVATTHT
                     QARYALPLIVADFVKRYPKVNLTIKQGSPAAIAQMVTSGESDLAIVTERIDDHPELGR
                     LSCYDWTHAVIVPNDHPLLECRNPLRIEDLARFPLITYEFAFNAGSSIARAFSKARLE
                     QPDVALAAADTDVLKTYVRLGLGVGLMAKMAYNPDTDGDLQLVDAAHLFEPSPTWIAL
                     RSDTYLRGYAYDFIQAFAPHLTREKVDRILYTPISEDFSI"
     gene            complement(6378..7112)
                     /gene="fnr"
                     /locus_tag="WGQ68_001487"
     CDS             complement(6378..7112)
                     /gene="fnr"
                     /locus_tag="WGQ68_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HEZ0715266.1"
                     /translation="MASHNTTHQMKTLCSSCSLRELCLPVGLLPNELSQLDAVIRQSR
                     RLKKGEYLFCVGEAFTSLFAIRSGFFKTTVASQDGRDQVTGFFMSGELIGMDGICSHV
                     HSCDAVALEDSEVCELPFTHIEELGQNIPSLRTHFFRMMSREIVRDQGVMLLLGNMRA
                     EERIAAFLLNLSQRLYSRGFAANDFILRMSREEIGSYLGLKLETVSRTLSKFHQEGLI
                     SVEHKHIKILNLQVLKKMVSGCSHAI"
     gene            7317..8738
                     /gene="hemN"
                     /locus_tag="WGQ68_001488"
     CDS             7317..8738
                     /gene="hemN"
                     /locus_tag="WGQ68_001488"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003684340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEZ0715267.1"
                     /translation="MKIIQIQNNHNVNDDRPEFDRALIASLPASGPRYTSYPTADRFH
                     DGFREGEYIKALHLRGMGALNKPLSLYIHIPFCNTICYYCGCNKIITKDKSRADAYIE
                     YLEKEMELLAPHLNGRHQLAQLHFGGGTPTFLSDEQIERVFRMIRKHFELIPTGEYSI
                     EIDPRKVSRDTVLMLGRLGFNRMSIGIQDFDPKVQAAVNRIQSYEETKEVIDAAREAG
                     FKSVSVDLIYGLPHQTSESIKTTIDTVLSLDPDRLALYHYAHLPHVFKPQRRIDTAAV
                     PDSEEKLDMLQYCVQTLTERGYVFIGMDHFAKPDDELSIALKEGFLQRNFQGYSTYAD
                     CDLVAIGVSSIGKIGSTYSQNERDIDAYYAAIDEGRLPIMRGYQLNQDDILRRNIIQD
                     LMCRFALDYRIYESMFGIPFDRYFKDELADLEKLAGLGLVRLNSHGLTVTPKGRFLIR
                     NIAMVFDYHLRHKETKAKYSQTV"
     gene            complement(9091..10665)
                     /locus_tag="WGQ68_001489"
     CDS             complement(9091..10665)
                     /locus_tag="WGQ68_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003748724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HEZ0715268.1"
                     /translation="MAQIQMSANVKTINAVFAAMLVGTVGYFIYWGLGYTHYNYAALF
                     IIATMFGVFMAFNIGGNDVANSFGTSVGAGTLTIPQALLIAAVFEVSGAVIAGGEVTN
                     TIRKGIVDLKGVDFEPIQFVFIMMSALLAAALWLLFASKKGLPVSTTHSIIGGIVGSA
                     VCMAVMNDAASGDLIRWGKLGGIGVSWVLSPVLGGAVSYFLFSRVKKNVLDYNAWAEG
                     TLKGIKQEKKAYKERHRLFFEGLSEAEKVEYATKMAHDAQIYDEPEFDPQELQSEYYR
                     GLYAFDNRKNNVDSYKALHSWIPFIASFGAMMISAMLIFKGLKNLHLGMSNVNSFLTI
                     FMIGAAVWMGTFVFAKSLKRKDLGKSTFQMFSWMQVFTACGFAFSHGANDIANAIGPF
                     AAIMDVLRTNSVAAQNVVPPIAMLTFGIALIVGLWFVGKEVIKTVGTSLAEMHPASGF
                     TAELSAASVVMGASLMGLPVSSTHILVGAVLGIGLVNRNANWKLMKPIGLAWVITLPA
                     AAVLSVVCYLVLQAVF"
     gene            10990..12090
                     /locus_tag="WGQ68_001490"
     CDS             10990..12090
                     /locus_tag="WGQ68_001490"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEZ0715269.1"
                     /translation="METQLYIGIMSGTSMDGADAVLIRMDGGKWLGAEGHAFTPYPDR
                     LRRKLLDLQDTGTDELHRSRMLSQELSRLYAQTVGELLNKQNLAPSDITALGCHGQTV
                     RHAPEHGYSVQLADLPLLAELTQIFTVGDFRSRDLAAGGQGAPLVPAFHEALFRDDRE
                     TRAVLNIGGIANISVLPPDAPAFGFDTGPGNMLMDAWMQAHWQLPYDKNGAKAAQGNI
                     LPQLLDRLLAHPYFAQPHPKSTGRELFALNWLETYLDGGENRYDVLRTLSRFTAQTVF
                     DAVSHAAADARQMYICGGGIRNPVLMADLAECFGTRVSLHSTAELNLDPQWVEAAAFA
                     WMAACWVNRIPGSPHKATGASKPCILGAGYYY"
BASE COUNT         3145 a         3382 c         2959 g         2627 t
ORIGIN      
        1 tgctttggaa aatccgaaat ggttgccggg gcggcgatcc cctatcatta ttattttttt
       61 gtctatataa tttcaaaggg ataagcggat tttatgaatc ctgcccgatt ttggcaatac
      121 cggttcgcgg ataaactggc ttaaatcaaa ttatcggtta aaatggccgt ctgaaatttg
      181 tttgatgaaa acgagaaaac catgtcccaa caatacgtct attctatgct gcgcgtgagc
      241 aaggttgtgc cgccgcagaa aaccatcatt aaagatattt ccctttcttt cttccccggc
      301 gcgaaaatcg gcctgctcgg tttgaacggc gcgggcaagt ccaccgtgct gcggattatg
      361 gcgggcgtgg ataaggaatt tgagggcgaa gccgtgccga tgggcggcat caaaatcggc
      421 tacctgccgc aagagcctga gcttgatccg gaaaaaaccg tgcgcgagga agtggaaagc
      481 ggtttgggcg aagtggctgc cgcgcagaaa cgtttggagg aagtgtatgc cgagtacgcc
      541 aatcctgatg cggattttga cgcgttggca gaagagcagg gccgcttgga agcgattatt
      601 gcggcaggtt cgtccacggg cggcggtgcg gaacacgaat tggaaatcgc cgccgacgcg
      661 ctgcgcctgc cggaatggga tgccaaaatc gataatttgt ccggcggtga aaaacgccgc
      721 gttgccttgt gcaaactctt gttgagcaag cccgatatgc ttttgctgga cgagccgacc
      781 aaccacttgg atgcggaatc ggtcgaatgg ctggagcaat ttctcgtgcg cttccccggc
      841 acagtcgttg cggtaacgca cgaccgctac ttcctcgaca atgccgccga atggattttg
      901 gaactcgacc gcggccatgg tattccgtgg aaaggcaatt actcgtcttg gctggagcag
      961 aaagaaaaac gcttggaaaa cgaggcaaaa tccgaagccg cgcgcgtgaa ggcgatgaag
     1021 caggaattgg aatgggtgcg ccaaaatgcc aaaggccgcc aagccaagtc caaagcgcgt
     1081 ttggctcgtt ttgaagaaat gagcaactac gaataccaaa aacgcaatga aacgcaggaa
     1141 atcttcattc ccgtcgccga gcgtttgggt aacgaagtga ttgaatttgt gaatgtttcc
     1201 aaatcgttcg gcgacaaagt gctgattgac gatttgagct tcaaagtgcc tgcgggcgcg
     1261 attgtcggca tcatcggtcc gaacggcgcg ggtaaatcga cactgtttaa aatgattgcg
     1321 ggcaaagagc agcccgattc cggtgaagtg aaaatcgggc aaaccgtgaa aatgagcttg
     1381 attgaccaaa gccgcgaagg tttgcaaaac gacaaaaccg tgttcgacaa cattgccgaa
     1441 ggtcgcgata ttttacaggt tggtcagttt gaaattcccg cccgccaata tttgggacgc
     1501 ttcaatttca aaggcagcga ccaaagcaaa atcgccggca acctttcagg tggcgaacgc
     1561 ggtcgtctgc acttggcgaa aaccttgttg ggcggcggca atgtattgct gttggatgaa
     1621 ccgtctaatg accttgacgt agaaaccctg cgcgcgctgg aagacgcatt gctggaattt
     1681 gccggtagcg tgatggtgat ttcgcacgac cgttggttcc tcgaccgtat tgctacgcat
     1741 atcttggctt gcgaaggcga ctccaaatgg gtgttctttg acggcaacta tcaggaatac
     1801 gaagccgaca agaaacgccg actcggcgaa gaaggcacga aaccgaaacg catcaaatac
     1861 aaaccggtaa cgcgttaacc tccgaaacaa tgccgtctga aaggctttca gacggcattt
     1921 ttacaaggca gcaccgttta aaacagcatt gcaatcctca agacaatcaa agtcatcacc
     1981 gcagccgcca tatcgtccgc cataatgccc aaaccgccgt gcagattctt gtcaaaccaa
     2041 ccgacgggag acggtttgag cgcgtcaaac agacggaata ggacaaatgc cgccagccac
     2101 cacgtccacc tgaacggcac aaacgccagc acaaacagca tggcgacaat ctcgtcccaa
     2161 acaatcccac catggtcgct gacacccgtt tcacgttccg cataagcgca aatgcgtatg
     2221 ccccacataa acagcacgat acacaaaaaa gccagcagca gcccgtctat gccgagcaaa
     2281 atcagcacaa atgccaaagg cagtgccgcc aaagtgccaa atgtacccgg cgcgaacgga
     2341 gccagcccgc tgccgaaacc aaaagccaaa aaacacaacg gtcgtttcaa cagccacgca
     2401 aagtcaggtt taaaatcagc caaaatgatc gaatcctaaa gaatgtagtt ccaattccct
     2461 gccgccgtca tctaaaactt tcagacggca tcctccgttg attttgccga tgcgcgttac
     2521 cggcacgccg caccgttctg ccgcatcaag cacgcggctg cggcaacttt ccggcgcggt
     2581 aaacaccagc tcgtaatcgt cgccgcccgc caaagtataa gacagccatt gcgctcgggg
     2641 caaaatatct ttcaatacgg ataaagacgg cagcgaatcg gcccaaattt ccgcccccac
     2701 gccggaagcg gtcaggatat gccccaaatc ttgcgcgatg ccgtctgaaa catcctgcgc
     2761 cgcccgggca aacggcaaca gcgcaagccc cagcccaacc cttggctcgg ggcggagcag
     2821 cttttgttcg cattcggcaa acacatcatc cggcaacaca caccgtttca gacggcagtt
     2881 caaagccgcc gccgccatac cgacacgccc cgacacccaa atatcgtcgc ccgcaaccgc
     2941 cgcatcacgc cgcaacgccc tacccttcgg caattcgccg ataatggtta cattgaacgc
     3001 catatcgccc ttggtcgtat cgccgccgat taacgttacg ccaaactttt ttgccaaacc
     3061 gaaaaagctg ccgcaaaacc gtttcagcca tacctcatcc aattcgggca aagccgcgct
     3121 cagcaacacc caacgcggta tcgcacccat cgccgccata tctgaaatat tgacggccaa
     3181 aaccttccaa gccaagtctt caggtttgac atctgcaaaa aaatgcctgt ccttcaaaag
     3241 catatccgca ctgaaacaca aatcgaagcc ttcacgcggg cggacaatcg ccgcatcgtc
     3301 gcctatgccc aatacgacat ccttatccgt gcctgtttgc aaataccgtt tgatgaagtc
     3361 aaattctttc atatcccaaa atatccggcg ggcgaatcca atttaaatca tagcaaggca
     3421 gcaaaggccg tacaaacaat aaagcggctc attcagtgtt tcagcgtatt tcacttttag
     3481 ctttggccat accgtacccg tatgcaccga ccaccctgtt tcaacacaca aacggcatat
     3541 caaagcaccg cactgcacgc caccccgaat gcaaagcggc aacttccgcc accgattagg
     3601 ctcaatcgct gcaactggcg aggtttcaac cgaaaagggc tgatgataaa accatatccg
     3661 gcatacagtg ccgcacacat ctggcacgat tccatccatt tcccaatgag ggggaaaggc
     3721 gtgaatttgt atgaattatc cctttcaggg cttgatacag aaaggattcc ctttctatgg
     3781 gcaaacccac atcctaccgc cttaaaatca gagtttcaac cgatatagtg gattaaattt
     3841 aaaccagtac agcgttgcct caccttagct caaagagaac gattctctaa ggtgctgaag
     3901 caccaagtga atcggttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
     3961 tttttgttaa tccactataa agcgcggcaa tgggacacaa acctgaatct gaatctgaat
     4021 ctgaatctga atccgaatcc gaatccgaac ctgcaccccg attctgacga tagccgtttc
     4081 agaaaaccac ccaaaaaaac aaaccccgca aaagcggggg tttgtttcac atcatcgatg
     4141 ccgaccgacg tttcacgaat attgcaacag tacccggtac aaaatccgct aacctcatgc
     4201 atcggcaaga tattgcctag ccttagtgtt ggtgatgatt gtgtgccggc acaacctgac
     4261 gggttacgat gctgcggatt ttcacatcca cacggcggtc aggttcgata catgcaatca
     4321 gagcctcacg ttttttggct ttagagactt tcgcacccag tttggcaact tcggcttcac
     4381 aaacttgagt catttgcgct tgagattcgc ccaagccaac agcagaaatt ctagaaacag
     4441 gtacgccgtt gctgaccagg ttgtttgcca ctacgtatgc gcggcgttcg gacagggcct
     4501 gattgtattt gtcagaaccc ataaagtcgg tatggccttc gacgcggaca gattggatat
     4561 tggtttgacc caggcgttgc gccaatactt tcaggttgtc ttgagcttcg gcgcgcaatg
     4621 aatccttatc gaaaccgaac agggttttgg cagacaggga aatggtttca tcaacatatt
     4681 gcggagcctg ctccacaacg acgacaggcg cgggtgcggg ttcgggttct ggctcgggtt
     4741 cgggggcagc aaccgcatcg ccgcattcta cgcgaccttg gcttgcttta tcaaagtagg
     4801 cgtttttcca gcattcgcca tagttgttgc gtacgatttc gttcgactgg ccgcttacgg
     4861 tgtaaccctg aacggacgcc tcgcccgcaa cagcagtgcc ggaagcgagc aatgcaacga
     4921 ataatgcgct taatttcagc tgtttggtca ttttattccc tcattaaatt tgtacagcag
     4981 atacagaagc aactgctgaa ttgccaacat acaggccgct gtagaaacat ggcggcaaat
     5041 cggatatttc agtctccact ataaagaagc agggggagga ctgtcaatac tcaacgcctg
     5101 aaaacaaccg taaaacagcc tgtttcctga tagtctatca tgccgaaaaa tcaattccgt
     5161 gtcattactt tagggggatt ttttccatat cgcacgattg ccgttgcaca aaaacaacaa
     5221 acacaacagc aaagccccat accgtaccgg caagaaaata tgataaatta taaacaatgt
     5281 tcagccaccc gacacagacc cacaccgacc cgccatgaaa ttacaacaat tgaaatacgc
     5341 cttagaagtt taccggcaca acctgaacgt ttccgaagcg gccgaagcct tgtttacttc
     5401 gcaacccggc atctccaaac aaatcaaatt gctggaagaa gaaatcggca ttcagatttt
     5461 tatccgcagc ggcaagcgcg tggtttcggt ctcgcagccg ggcaaggtgg ttttggatat
     5521 tgcagaacgt attttgcgcg atgttcagaa cattaaaaat atcggcagcg agtttaccgg
     5581 acaggacagc ggttcgctga cggttgccac gacgcatact caggcacgct atgccctacc
     5641 cttgattgtt gccgattttg tgaaacgcta tccgaaagtc aacctgacca tcaaacaggg
     5701 gagccctgcc gccatcgccc aaatggttac ttcgggagag tctgatttgg caattgttac
     5761 ggaacgtata gacgaccacc ccgaactggg aagactttcc tgctacgact ggacacacgc
     5821 ggttatcgta ccgaacgacc accccttgct cgaatgcaga aaccccctcc gtattgaaga
     5881 tttggcgagg tttccgctga ttacttatga atttgcattc aatgcgggca gcagcatcgc
     5941 gcgggcattt tccaaagccc gtttggaaca gccggatgtc gcattggcgg cggcggatac
     6001 ggacgtattg aagacttatg tgcgcttggg tttgggcgtg ggactgatgg cgaaaatggc
     6061 gtacaacccg gatacggacg gcgatttgca gcttgtggat gcggcacacc tgttcgagcc
     6121 gtcgccgacg tggattgctt tgcgcagcga tacttatttg cgcggatatg cctacgactt
     6181 tatccaagcg ttcgcgccgc acctgacacg cgagaaggtg gataggattc tgtacacgcc
     6241 catcagcgag gatttttcga tttaggcggc tgccggtttt cagacggcac tttgcggcag
     6301 atacaacaaa caggacagat gttttcgtct gccctgtgtt tattgagaat gctgtctgaa
     6361 atgttcgtac gggttaatca aatggcgtgc gagcagccgg acaccatttt tttcaacacc
     6421 tgcagattga ggattttgat gtgcttatgc tcgacggaaa tcaatccttc ctgatgaaat
     6481 ttagataatg tgcggctgac ggtttcaagt ttcagcccga gataactgcc gatttcttcg
     6541 cgggacattc ttaagatgaa gtcgttggca gcaaaacctc gggaataaag gcgttgggaa
     6601 aggttcagca ggaaggcggc aatccgctct tcggcgcgca tattgcccaa cagcagcata
     6661 acaccttggt cgcgcacgat ttcacggctc atcatgcgga agaagtgcgt acgcaggctg
     6721 gggatgtttt gccccagttc ttcgatgtgg gtaaacggca gttcgcacac ttcgctgtct
     6781 tccaaggcga ccgcgtcgca actgtgcaca tgggaacaga tgccgtccat gccgatgagt
     6841 tcgcccgaca taaagaaacc cgttacctga tcgcggccgt cctgactggc gacggttgtt
     6901 ttgaagaagc ccgaacggat ggcaaagagc gaggtaaagg cttcgccgac acagaacagg
     6961 tattcgccct ttttcaggcg gcggctttga cggatgacgg catcgagttg gctgagctcg
     7021 ttgggcagca gcccgacagg caggcagagt tcccgcaaag aacaggaaga acacagcgtt
     7081 ttcatctgat gtgtagtatt atgcgaagcc ataccgtacc tttttgtgcg ctttgcccca
     7141 tcatgattat agtggattaa atttaaacca gtacggcgtt gcctcgcctt gtccttattt
     7201 aaatttaatc cactatatgt gcttattgac acatatcaag acaggtttat catactgtgg
     7261 cattctacca aactatccaa aatttgacta acatcataaa accgcacaca cacaccatga
     7321 aaatcattca gatacagaac aatcacaatg taaacgatga ccgccccgag tttgaccgcg
     7381 cgctgattgc cagcctgccc gccagcggcc cgcgctacac ttcctaccct accgccgacc
     7441 gtttccatga cggtttccgc gaaggcgaat atatcaaagc tttacatttg cgcggtatgg
     7501 gcgcgttaaa caaaccgctt tccctttaca ttcacattcc gttctgcaac accatctgct
     7561 actactgcgg ctgcaacaaa atcatcacca aagacaaaag ccgcgccgat gcctacatcg
     7621 aatatcttga aaaagaaatg gaactgctcg ctccacatct gaacggacgg caccagcttg
     7681 cccaactgca cttcggcggc ggcacgccga cctttttgag cgacgaacag atcgaacgtg
     7741 tcttccgcat gatacgcaaa catttcgagt taatccccac cggcgaatac tccatcgaaa
     7801 tcgacccgcg caaagtcagc cgcgacaccg tcctcatgct cggcagactc ggcttcaacc
     7861 gcatgagcat cggcattcag gatttcgacc ccaaagtgca ggcggcggtc aaccgcatcc
     7921 aaagttacga agaaaccaaa gaagtcatcg atgcggcgcg cgaagcgggg ttcaaatccg
     7981 tcagcgtcga tttgatttac ggcctgccgc accagacttc ggaaagcatc aaaaccacca
     8041 tcgataccgt tttgtcgctc gatcccgacc gcctcgccct ttatcactac gcccacctgc
     8101 cgcacgtgtt caaaccgcaa cgccgcatcg ataccgccgc cgttcccgac agcgaagaga
     8161 agctcgatat gctgcaatac tgcgtccaaa ccctaaccga acgcggctac gtcttcatcg
     8221 gcatggatca tttcgccaaa cctgacgacg aactctccat cgccctcaaa gaaggcttcc
     8281 tccagcgcaa cttccaaggc tattcgacct acgcggattg cgatttggtc gccatcggcg
     8341 tgtcgtccat cggcaaaatc ggcagcacct attcccaaaa cgaacgcgac atcgatgcct
     8401 actatgccgc catcgacgaa ggcagactgc ccatcatgcg cggttatcag ctcaatcagg
     8461 acgacatcct gcgccgcaac atcattcagg atttgatgtg ccgtttcgcg ctcgactacc
     8521 ggatttacga aagtatgttc ggcatcccgt tcgaccgcta cttcaaagac gaactggcgg
     8581 atttggaaaa actcgccggt ttgggattgg tgcgcctgaa cagccacggg ctgaccgtta
     8641 ccccgaaagg acgcttcctc atccgcaaca tcgccatggt cttcgattac cacctgcgcc
     8701 ataaagaaac caaggcgaaa tattcgcaaa cggtgtgatt gcgtctaacg tacaaatgcc
     8761 gtctgaaagg ctttttcaga cggcattttg ctgccggcag gataagtgtt ttcaagaaca
     8821 ggcggcggca tatcataacg ttccgcacct ttgtgtccga ccgttccgaa accaagatat
     8881 agtggattaa caaaaaccag tacagcgttg cctcgccttg ccgtactatc tgtactgtct
     8941 gcggcttcgt cgccttgtcc tgatttttgt taatcactat accaaacggc atatcccgac
     9001 agaacagatt gcgcataagg cacaagcccg cacattccat caacaaaatg ccgtctgaac
     9061 acgggttcag acggcatcag tattttacaa tcagaatact gcctgtaaaa ccaagtagca
     9121 gacaaccgac aatacggcgg cggcaggcag ggtaatgacc cacgccaaac cgatgggctt
     9181 catcagtttc cagttggcat tgcggttgac cagaccgatg ccgagtaccg cgccgaccaa
     9241 gatatgcgta ctggacacgg gcagccccat cagcgacgcg cccatcacga cggatgcggc
     9301 ggacagttcg gcggtaaaac ccgaagcagg atgcatttcc gccaaactcg taccgacggt
     9361 tttaatcacc tctttaccga caaaccacaa accgacaatc agcgcgatac cgaaagtcag
     9421 catcgcaatc ggggggacga cattttgcgc ggcaacgctg ttggtacgca aaacatccat
     9481 aatcgcggca aacggaccga tggcgttggc gatatcgttc gcaccgtggc tgaatgcgaa
     9541 gccgcaggcg gtaaagacct gcatccatga aaacatctga aaggtcgatt tgcccaagtc
     9601 tttacgcttg aggcttttgg caaacacaaa cgtccccatc cacaccgccg cgcctatcat
     9661 aaagatggtc aggaagctgt tgacgttgct catccccaaa tgcaggtttt tcaagccctt
     9721 gaaaatcagc atagcggaaa tcatcatcgc gccgaacgaa gcgataaagg gaatccaaga
     9781 atgcagcgct ttgtaggaat cgacattgtt tttacggttg tcgaacgcat aaagaccgcg
     9841 gtaatactcc gattgcagct cttgcggatc gaattcgggt tcgtcgtaaa tttgcgcgtc
     9901 gtgcgccatt ttggtggcgt actcgacttt ttcggcttcg gacaaaccct cgaaaaacag
     9961 gcggtgccgt tctttatagg cctttttttc ctgcttgatg cccttgagcg tgccttccgc
    10021 ccaagcgttg taatctaaga cgtttttctt gacgcgcgaa aacaggaaat aggacaccgc
    10081 gccgcccaac acgggcgaca atacccaaga aacaccaata ccgcccagct tgccccaacg
    10141 tatcaaatcg cccgatgcgg catcgttcat taccgccata cataccgcgc tgccgacaat
    10201 gccgccgata atggaatggg tggtagatac cggaagccct tttttcgaag caaacaacag
    10261 ccacaacgcc gccgccaaaa gcgcggacat cataataaac acaaactgta tgggttcgaa
    10321 atcaacaccc ttcaaatcga cgatgccttt gcgtatggta ttggttacct cgccgcccgc
    10381 gatgaccgcg ccgctgacct caaataccgc cgcaatcagc aaagcctgcg ggatggtcag
    10441 cgtacccgca ccgacgctgg tgccgaaaga attggcaaca tcgttgccgc cgatgttgaa
    10501 cgccataaac acgccgaaca tcgtggcaat aatgaataag gcggcgtaat tgtaatgggt
    10561 ataacccaag ccccaataaa taaaatagcc gactgtacct accagcatgg cggcaaagac
    10621 ggcgttgatg gttttaacat ttgcgctcat ttggatttga gccatggcaa tcttccttta
    10681 atgtggtgga aatcatgagt gttgccgaca ctgtactgct gcctccggtc gaactgccgt
    10741 ttcgggatat ggcagacgca tccttccttc tgaaccgcat ccgaataccc ccaaacctga
    10801 ccgaatgcaa aactgctgcc ggaaacggtt tgtatgacac ttctactact ggatgttgcg
    10861 ggcgcattat aatatttttc catccgtctt gaaacattta tttacacttt atttacactg
    10921 cggcggcaaa tcggtatacg agcgtcaata cacgttaaaa tggcgttttg caccagtttg
    10981 ggagtgatga tggaaacaca gctttacatc ggcatcatgt cgggaaccag catggacggg
    11041 gcggatgccg tactgatacg gatggacggc ggcaaatggc tgggcgcgga agggcacgcc
    11101 tttaccccct accccgacag gctgcgccgc aaattgctgg atttgcagga cacaggcaca
    11161 gacgaactgc accgcagcag gatgttgtcg caagaactca gccgcctata tgcgcaaacc
    11221 gtgggcgaac tattaaacaa gcaaaacctc gcgccgtccg acattaccgc cctcggctgc
    11281 cacggtcaaa ccgtccgaca tgcaccggaa cacggttaca gcgtacagct tgccgatttg
    11341 ccgctgctgg cggaactgac tcagattttt accgtcggcg acttccgcag ccgcgacctt
    11401 gcggccggcg gacaaggcgc gccgctcgtc cccgcctttc acgaagccct gttccgcgac
    11461 gacagggaaa cacgcgcggt actgaacatc ggcgggattg ccaacatcag cgtactcccc
    11521 cccgacgcac ccgccttcgg cttcgacaca ggaccgggca atatgctgat ggacgcgtgg
    11581 atgcaggcac actggcagct tccttacgac aaaaacggtg caaaggcggc acaaggcaac
    11641 atattgccgc aactgctcga caggctgctc gcccacccgt atttcgcaca accccaccct
    11701 aaaagcacgg ggcgcgaact gtttgcccta aattggctcg aaacctacct tgacggcggc
    11761 gaaaaccgat acgacgtatt gcggacgctt tcccgattca ccgcgcaaac cgttttcgac
    11821 gccgtctcac acgcagcggc agatgcccgt caaatgtaca tttgcggcgg cggcatccgc
    11881 aatcctgttt taatggcgga tttggcagaa tgtttcggca cacgcgtttc cctgcacagc
    11941 accgccgaac tgaacctcga tccgcaatgg gtagaagccg ccgcgttcgc atggatggcg
    12001 gcgtgttggg tcaaccgcat tcccggtagt ccgcacaaag caaccggcgc atccaaaccg
    12061 tgtattctgg gcgcgggata ttattattga gcacaaatgc cgtctgaaag acc
//
LOCUS       DAWFHY010000062        12105 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.062, whole genome shotgun sequence.
ACCESSION   DAWFHY010000062 DAWFHY010000000
VERSION     DAWFHY010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.062"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(475..2109)
                     /gene="groL"
                     /locus_tag="WGQ68_001491"
     CDS             complement(475..2109)
                     /gene="groL"
                     /locus_tag="WGQ68_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271863.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEZ0715270.1"
                     /translation="MAAKDVQFGNEVRQKMVNGVNILANAVRVTLGPKGRNVVVDRAF
                     GGPHITKDGVTVAKEIELKDKFENMGAQMVKEVASKTNDVAGDGTTTATVLAQSIVAE
                     GMKYVTAGMNPTDLKRGIDKAVAALVEELKNIAKPCDTSKEIAQVGSISANSDEQVGA
                     IIAEAMEKVGKEGVITVEDGKSLENELDVVEGMQFDRGYLSPYFINDAEKQIAALDNP
                     FVLLFDKKISNIRDLLPVLEQVAKASRPLLIIAEDVEGEALATLVVNNIRGILKTVAV
                     KAPGFGDRRKAMLQDIAILTGGTVIAEEVGLSLEKATLDDLGQAKRIEIGKENTTIID
                     GFGDAAQIEARVAEIRQQIETATSDYDKEKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KDRVEDALHATRAAVEEGVVAGGGVALLRARAALENLHTGNADQDAGVQIVLRAVESP
                     LRQIVANAGGEPSVVVNKVLEGKGNYGYNAGSGEYGDMIEMGVLDPAKVTRSALQHAA
                     SIAGLMLTTDCMIAEIPEDKPAVPDMGGMGGMGGMM"
     gene            complement(2202..2492)
                     /locus_tag="WGQ68_001492"
     CDS             complement(2202..2492)
                     /locus_tag="WGQ68_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEZ0715271.1"
                     /translation="MTIRPLHDRVVVKRLEAEEKTASGIVLPGAAAEKPDMGEVIAVG
                     AGKIGKDGARRPLDVKVGDKIIFGKYSGQTVKADGEELLVMREEDIFGIVEK"
     gene            2721..3374
                     /locus_tag="WGQ68_001493"
     CDS             2721..3374
                     /locus_tag="WGQ68_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0715272.1"
                     /translation="MKITHCKLKKKVQKELLRFFVLEVTARSAADILGIHPNLAVLFY
                     RKIRTVINHHLALAADEVFEDPVEPDESDFGGWRKGRCGRGAAGKVVVFDILKRNGRV
                     YTVVVDNAKSETLLPVIKKKIMPDSIVYTDSLSSCDKLDVSGFIYYRINHSKEFADRQ
                     NHINGIENFWNQAKRVLRKYNGIDRKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            3587..5122
                     /locus_tag="WGQ68_001494"
     CDS             3587..5122
                     /locus_tag="WGQ68_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEZ0715273.1"
                     /translation="MSDSKTKERATFGTRRAFMIAAIGSAVGLGNIWRFPYIAFENGG
                     GAFILPYLVALLTAGIPLLLLDYAIGHRYRGSAPLAFRRLGRWFEPVGWWNVMTNIVI
                     CIYYAVIIGWAASYTYYSVNAAWGADPQGFFFKDFLQMAGPEALGLDFVGKVAGPLAG
                     VWVFTAAIMALGVQKGVARASSFFMPLLLVMFLIMVGISLTLPGAAKGLDALFTPDWS
                     KLADSKVWVAAYGQIFFSLSICFGIMVTYSSYLKKKTDLGGTGLVVGFANSSFELLAG
                     IGVFAALGFMAQAGGKAVNEVASGGIGLAFIAFPTIINQAPMGWLIGILFFGSLVFAG
                     VTSMISILEVIVAAIQDKLNIGRVNATLLVCIPMGIVSTLLFGTATGLPVLDVMDKFV
                     NTYGIVAAGFVYVAAIIISGRLPELRKHLNALSSIRIGGLWTVCVVVTVVMLGYMLFK
                     DTSGLMEKNYEGYPDGFLSIFGWGMSAALVVFGLLLSLLPWKHGQDFNVKDEHEHEQG
                     EEK"
     gene            5119..5211
                     /locus_tag="WGQ68_001495"
     CDS             5119..5211
                     /locus_tag="WGQ68_001495"
                     /inference="COORDINATES: protein motif:HMM:NF033493.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine/alanine import family NSS transporter
                     small subunit"
                     /protein_id="HEZ0715274.1"
                     /translation="MSTSAIVMMIVSIVIIWGGLLLSLLRLPNE"
     gene            complement(5271..6491)
                     /gene="lysA"
                     /locus_tag="WGQ68_001496"
     CDS             complement(5271..6491)
                     /gene="lysA"
                     /locus_tag="WGQ68_001496"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEZ0715275.1"
                     /translation="MTLFCEQVPYPRLAEAFGTPLYVYSQSALTEAFEHYQTAFAALN
                     PLVCYAVKANGNLSIIKHFASLGSGFDIVSGGELARVLAAGGDAAKTIFSGVGKSEVE
                     IEFALNAGVKCFNMESIPEIDRIQKVAARLGKTASVSLRVNPDVDAKTHPYISTGLKA
                     NKFGIAYADALEAYRHAAQQPNLKIIGIDCHIGSQLTDLSPLVEACERILILVDALAA
                     EGIVLEHLDLGGGVGIVYQNEKVPDLGAYAQAVQKLIGTRRLKLVLEPGRSLVGNAGA
                     LLTRVEFVKHGEEKNFVMVDAAMNDLMRPALYDAYHHIEAVETKDIEPLTANIVGPIC
                     ETGDFLGKDRTIACEEGDLLLIRSAGAYGASMASNYNARNRAAEVLVDGNEYRLIRRR
                     ETLEQQMANELACL"
     gene            complement(6502..6672)
                     /locus_tag="WGQ68_001497"
     CDS             complement(6502..6672)
                     /locus_tag="WGQ68_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0715276.1"
                     /translation="MKYGVFFAAATALLLSACGYKGDLYLPKEGDKARFGVIQTGLQL
                     QGKPQSAPPTQK"
     gene            6743..7066
                     /gene="cyaY"
                     /locus_tag="WGQ68_001498"
     CDS             6743..7066
                     /gene="cyaY"
                     /locus_tag="WGQ68_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016686572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HEZ0715277.1"
                     /translation="MMTESEFIRASEALFEHIEDQIDENGWDFDCRFAGNVLTIEAEN
                     GTQIIVNRHTPNQELWIAAKSGGYHFAEQNGKWLATRDSRDFYDVLNEALSAASGEAV
                     EIEEL"
     gene            7093..8109
                     /locus_tag="WGQ68_001499"
     CDS             7093..8109
                     /locus_tag="WGQ68_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HEZ0715278.1"
                     /translation="MNTRPFYFGLIFIAIIAILANYLGNTDFSHHYHISALIIAILLG
                     MAIGNTIYPQFSTQVEKGVLFAKGALLRTGIVLYGFRLTFGDIADVGLNAVVTDAIML
                     ISTFFFTVLLGIRYLKMDKQLVYLTGAGCSICGAAAVMAAESVTKAESHKASVAIAIV
                     VIFGTLAIFTYPLFYTWSQDLINAHQFGIYVGSSVHEVAQVYAIGENIDPIVANTAVI
                     SKMIRVMMLAPFLLMLSWLLTRSDGVSENTSHKITIPWFAVLFIGVAIFNSFDLLPKE
                     LVKLFVEIDSFLLISSMAALGLTTHASAIKKAGLKPFVLGILTYLWLVVGGFLVNYGI
                     SKLI"
     gene            8135..8554
                     /locus_tag="WGQ68_001500"
     CDS             8135..8554
                     /locus_tag="WGQ68_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2251 domain-containing protein"
                     /protein_id="HEZ0715279.1"
                     /translation="MAQLPLYRTAEIENFTVGTPEVLESFSKHIPYGVVFEDDGDTGY
                     FYAASQDGILDALHIYNVEDVSDKHIPNHVLILWDDACTIAALCINDYIHAVYDFVEQ
                     AGYCRNGFPEAQGEWPKVTDRVLDDELQDKILSRKPT"
     gene            8576..9082
                     /gene="luxS"
                     /locus_tag="WGQ68_001501"
     CDS             8576..9082
                     /gene="luxS"
                     /locus_tag="WGQ68_001501"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEZ0715280.1"
                     /translation="MPLLDSFKVDHTRMHAPSVRVAKTMTTPKGDTITVFDLRFCVPN
                     KEILPEKGIHTLEHLFAGFMRDHLNGNGVEIIDISPMGCRTGFYMSLIGTPSEQQVAD
                     AWLASMQDVLNVKDQSKIPELNEYQCGTYLMHSLAEAQQIAQNVLARKVAVNKNEELM
                     LDEGLLNA"
     gene            9228..12020
                     /gene="polA"
                     /locus_tag="WGQ68_001502"
     CDS             9228..12020
                     /gene="polA"
                     /locus_tag="WGQ68_001502"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEZ0715281.1"
                     /translation="MSNRPTLLLVDGSSYLYRAYHAMGQNLTAPDGAPTGALYGVLNM
                     LRRLRSEYPHDYCAVVFDAKGKNFRHQMFEEYKATRPPMPDDLRPQAEALPDLVRLTG
                     WPVLVIGQVEADDVIGTLAKQGEEHGLRVIVSTGDKDMAQLVNERVTLVNTMSGETLD
                     IEGVKEKFGVRPDQIRDYLALMGDKVDNVPGVEKCGPKTAVKWLEAYGSLAGVMEHAG
                     EVKGKVGENLRAALPRLPLSYDLVTIKTDVDLHTELSDGIESLRRTTPKWAQLVVDFK
                     RWGFRTWLKEAESRMHEAVDGDLFGSDSIGEQAALDVEMPSERLSEKAAVPEKLDYQA
                     VTTEAQFAALLDKLSQADKIGIDTETTSLDAMNAALVGISIAFQAGEAVYIPVGHSLT
                     AAPEQLDLQDVLGRLKPHLENPALKKIGQNLKYDQHVFANYDIALNGIAGDAMLASYI
                     IESHLGHGLDELSERWLGLETITYESLCGKGAKQIGFADVAIGQATEYAAQDADFALR
                     LEAHLRAQMDEKQLEMYEKMELPVAQVLFEMERNGVQIDRAELARQSAELGAELMKLE
                     QQAYAAAGQPFNLNSPKQLQEILFDKMGIPTKGLKKTAKGGISTNEAVLEQLAPDYPL
                     PKIILQNRSLAKLKSTYTDKLPEMISPKDGRVHTTYAQAVAITGRLASNNPNLQNIPI
                     RTAEGRRVRRAFTAPQGSVIVSADYSQIELRIMAHLSGDKTLIAAFQNGEDVHRRTAA
                     EVFGIAPENVSSEQRRYAKTINFGLIYGMGQYGLAKSLGIDNISAKNFIDRYFARYPG
                     VAEYMQRTKEQAAAQGYVETLFGRRLYLPDIRNKNANARAGAERAAINAPMQGTASDL
                     IKRAMIDVRNWLSDGIGSKLVMQVHDELVLEVVETELDFVKEKLPQIMAKVDGGLLDV
                     PLVAEVGVGENWEEAH"
BASE COUNT         2810 a         3125 c         3154 g         3016 t
ORIGIN      
        1 tccgataaat tcttgaggca ttgaaattcc agattcccgc ctgcgcggga atgacgaatc
       61 catccgcaca gaaacctgca ccacgtcatt cctacgaacc tacatcccgt cattcccaca
      121 aggacagaaa accaaaatca gaaacctaaa atcccgtcat tcccacgaaa gtgggaatcc
      181 agaacgtaaa atctgaagaa accgttttat ccgatacgtt tccgcaccga cagacctaga
      241 ttcccgcttt cgcgggaatg acggcggaaa ggttgctgtt tttccgataa attcctgccg
      301 ctcttcgttt ttgggatggc gggaaataaa acaaaagcgc gcgtatcaaa aaataacaat
      361 gcaaagaacg gtgttggcaa ttttaaggtt tgcgccttag ccgcacttat tcgcaaaaaa
      421 accgcacggc gttgaccgtg cggcttgttg tctgaaggct tcagacggca ttgcttacat
      481 catgccgccc ataccaccca tgccgcccat atcaggcaca gccggtttgt cttcggggat
      541 ttcagcgatc atgcaatcag tggtcagcat caagccggcg atggaagcgg cgtgttgcag
      601 cgcggaacgg gttactttgg cggggtcgag tacgcccatt tcgatcatgt cgccgtattc
      661 gccggagcct gcgttgtaac cataattgcc tttgccttcc aacactttgt tcaccaccac
      721 gctgggttcg ccgcctgcgt tggcaacgat ttggcgcagc ggagactcaa cggcgcgcaa
      781 gacgatttgt acgcctgcgt cttggtcggc attgccggtg tgcaggtttt ccaaagcagc
      841 acgggcgcgc aacagggcta cgccgccgcc tgcaaccacg ccttcttcaa cggctgcgcg
      901 ggtagcgtgc agcgcgtctt ccacgcggtc ttttttctct ttcatttcaa cttcggtcgc
      961 cgcgccgact ttgatcactg ccacgccgcc tgccagtttg gcaacgcgct cttgcagttt
     1021 ttctttgtcg taatcgctgg ttgcggtttc gatttgttgg cggatttcgg caacacgcgc
     1081 ttcgatttgg gctgcgtcgc cgaagccgtc gatgatggtg gtgttttctt taccgatttc
     1141 gatgcgtttg gcttgaccca aatcgtccaa agtcgctttt tccagagaca ggccgacttc
     1201 ttcggcaatt acggtgccgc cggtcaggat ggcgatgtct tgcaacatgg ctttacgacg
     1261 gtcgccgaag ccaggggctt taacggcaac ggttttcagg atgcctcgga tgttgttcac
     1321 caccaaagtc gccaaggctt cgccttccac gtcttcagcg ataatcaaga gcgggcggct
     1381 ggctttggcc acttgttcca aaacaggcaa caaatcgcgg atattgctga tttttttgtc
     1441 gaacaacaat acaaacggat tgtccaaagc agcgatttgt ttttcagcgt cgttgatgaa
     1501 gtaaggagac aggtagccgc ggtcgaactg cataccttca accacatcca gctcgttttc
     1561 caaagatttg ccgtcttcaa cggtaatcac gccttctttg ccgacttttt ccatcgcttc
     1621 ggcgataatc gcgccgactt gttcgtcaga gttggcggaa atcgagccga cttgggcgat
     1681 ttctttagaa gtgtcgcaag gtttggcgat gttttttagc tcttcaacca aagcggcaac
     1741 ggctttgtcg ataccgcgtt tcaggtcggt cgggttcata ccggcggtaa cgtatttcat
     1801 accttcggca acgatggatt gcgccaatac ggtggcggta gtcgtaccgt cgcctgccac
     1861 gtcgttggtt ttggacgcga cttctttcac catttgcgcg cccatatttt caaacttgtc
     1921 tttcagttcg atttctttgg cgacggttac gccgtctttg gtgatgtgcg ggccgccgaa
     1981 agcgcggtca acgactacgt tgcggccttt ggggcccaag gttacgcgca cggcgtttgc
     2041 caaaatgttc acgccgttta ccattttttg gcggacttca ttgccgaact gtacgtcttt
     2101 tgctgccatt tcaattctcc aaaaatcatt aaaactgtct gataaaaccg tttatgccgt
     2161 ctgaaggcgg tttgccgttt cagacggcat cgtgtccgta tttatttttc aacgatgccg
     2221 aaaatatctt cttcacgcat taccaacagc tcttcgccgt cggctttgac ggtttggccg
     2281 ctgtatttgc cgaaaatgat tttgtcgccg actttgacat ccagcggacg gcgcgcgccg
     2341 tctttgccga ttttgcccgc gcccacggcg atgacttcgc ccatatcggg tttttcggcg
     2401 gccgcacccg gcaaaacgat gcccgatgcg gttttctctt cagcttccaa gcgtttgacg
     2461 acaacgcggt cgtgtaaagg acggatggtc atatttatgc tccgataaaa tgatttgaaa
     2521 acaatcatct gcccgaacgg ttcggacaga ttgaagtgga aaccgggctg ccgcctgccg
     2581 gcccgcccgt ataagtcggc aaaattaggg tgtgtgcggg caaattcaag tgaggcggaa
     2641 aaaatttatt tccgccgttt tttatagtgg actaaattta aggggctgta ctagattagc
     2701 agatatgtta ccctcgaaat atgaagataa cgcactgcaa attaaagaaa aaagtacaga
     2761 aagaactgct ccgttttttt gtactggaag ttaccgcccg ttctgccgcc gatattttgg
     2821 gtatccatcc caatttggca gtactgttct accgtaaaat ccgcacggtt atcaaccatc
     2881 atttggccct ggctgccgat gaggtttttg aggatcctgt cgagccggac gaaagcgatt
     2941 tcggcgggtg gcgtaaaggc agatgtggtc gtggtgcggc aggaaaagtg gttgtcttcg
     3001 acattctgaa acgcaacgga cgggtctata ccgttgtggt ggataatgcc aagtctgaaa
     3061 cgttactccc tgtcatcaaa aagaaaatca tgccggacag tattgtttat accgatagtc
     3121 tgagcagctg cgacaagttg gacgtgagcg gtttcattta ttaccgcatc aaccattcca
     3181 aggaatttgc agaccgtcag aaccacatta acggcattga gaatttttgg aatcaggcaa
     3241 aacgtgtctt gcgaaaatac aatggaatcg atcgtaaatc tttcccgctg ttcttgaaag
     3301 aatgcgaatt tcgatttaac ttcggcacac cgtctcaaca gcttaaaatc ctgcgggatt
     3361 ggtgtgggat ttagggctaa tctagtacag cccctaaaat ttttcgtttt caagccttca
     3421 ccgcttgcca tcagcgttaa atttttttac gataagcaca tagattgtaa acaatcggcc
     3481 acaagccggt ttgttttttc agaagacatt atccctgtca gacgctattt ctatatattt
     3541 cgcctataat ggcttgtttt taataaataa ttcaagaggt atcaacgtgt ctgattccaa
     3601 gacgaaagaa cgcgccacat tcggcacgcg ccgcgcgttt atgattgccg ccatcgggtc
     3661 cgccgtcggc ttgggcaata tttggcgttt cccctatatt gcttttgaaa acggcggcgg
     3721 cgcgttcatc ctgccctatc tggtcgcgct tctgacggcg ggcatcccgc tgctgctgct
     3781 cgattatgcc atcggccacc gttaccgtgg ttctgcgccc ttggctttcc gccgcctcgg
     3841 acgatggttt gagccggtcg gctggtggaa cgtgatgacc aatatcgtca tctgcatcta
     3901 ttacgcggta attatcggtt gggcggcaag ctatacctat tattcggtca acgccgcctg
     3961 gggtgcggat ccgcagggtt ttttctttaa ggacttcctg caaatggcgg gcccggaagc
     4021 cttgggtttg gattttgtcg gcaaagtcgc cggtcctttg gcgggcgtgt gggtttttac
     4081 cgccgccatt atggcgttgg gcgtgcaaaa gggcgtggcg cgcgcctcgt cgttctttat
     4141 gccgctgctt ttggtgatgt ttttgattat ggtcggcatt tcactaaccc tgccgggtgc
     4201 ggcaaagggc ttggacgcat tgtttacgcc cgactggtcg aaactcgccg attccaaggt
     4261 ctgggtggcg gcatacgggc agattttctt ttcgctttcc atctgcttcg gcattatggt
     4321 tacctattct tcttatttga agaaaaaaac cgacttgggc ggaacggggc tggtggtcgg
     4381 ttttgccaac agcagctttg aactgctcgc gggcatcggc gtgtttgccg cattgggctt
     4441 tatggcgcag gcgggcggta aggcggtcaa cgaggttgcc tcaggcggca tcggtttggc
     4501 gtttatcgcc tttccgacca ttatcaacca ggcaccgatg ggctggctga tcggcatatt
     4561 gtttttcggt tcgctggtat tcgccggcgt tacgtcgatg atttccatcc ttgaagtgat
     4621 tgtggcggcg attcaggaca agctgaacat cgggcgcgtc aacgccacgc tgctggtctg
     4681 cattccgatg ggcattgttt ccacgctgct gttcggtacg gcgacggggc tgccggtttt
     4741 ggacgtgatg gacaaattcg tcaacaccta cggcattgtt gccgccggct ttgtttatgt
     4801 tgccgccatc atcatcagcg gcaggctgcc ggaattacgc aagcacctga acgctttgtc
     4861 ctccatccgc atcggcggct tgtggacggt ctgcgtcgtg gttaccgtcg tgatgctcgg
     4921 ctatatgctg tttaaagata ccagcggcct gatggagaaa aattacgaag gttatccgga
     4981 tggtttcctc agtattttcg gctgggggat gtcggcggcg ttggtcgtgt tcgggctgct
     5041 gctgtcgttg ctgccttgga aacacggtca ggatttcaac gtcaaagacg aacacgaaca
     5101 tgaacaagga gaagaaaaat gagtacttcc gccattgtga tgatgattgt ctcaatcgtg
     5161 ataatctggg gagggctgct gctttccctg ttaaggctgc cgaacgagta agcctttaga
     5221 gcgttaaaaa tgccgtctga actgtttcag acggcatttt gatgttcggc ttacaggcag
     5281 gcgagttcgt ttgccatttg ctgttccaag gtttcgcgcc ggcggatgag tcggtattcg
     5341 ttgccgtcca ccaacacctc tgccgcacgg ttgcgcgcgt tgtaattgct cgccatactg
     5401 gctccgtatg cgcccgcgct gcggataagc agcaaatccc cttcttcgca ggcgatggtg
     5461 cggtctttgc cgaggaagtc gccggtttcg caaatcgggc cgacgatatt ggccgtcaga
     5521 ggctcaatgt ctttggtttc aaccgcttca atgtgatgat aggcatcata tagagcgggg
     5581 cgcatgaggt cgttcatcgc cgcatcgacc atcacaaagt ttttctcttc gccatgtttg
     5641 acgaattcga cgcgcgtcaa caacgcacct gcattgccga ccaaactgcg gcctggctcg
     5701 agaaccagct tcagacggcg tgtgccgatc agtttttgaa ccgcttgggc atacgcgccc
     5761 aaatcaggca ctttttcgtt ttggtaaaca atgccgacgc cgccgcctaa gtccaaatgt
     5821 tccaaaacaa tgccttcggc ggcaagcgcg tcaaccaaaa tcaaaatgcg ttcgcaggct
     5881 tcgaccagcg ggcttaagtc ggttagttgg gagccgatgt ggcagtcgat gccgatgatt
     5941 ttcaaattgg gctgttgtgc ggcatggcgg taggcttcga gcgcgtcggc gtaggcgata
     6001 ccgaatttgt tggctttcaa acctgtggag atgtaaggat gggttttcgc atcgacatcg
     6061 ggattgacgc gcagggagac ggacgcggtt ttacccaaac gcgcggcaac tttttgaatg
     6121 cggtcgattt cggggatgct ttccatattg aagcatttca cgcccgcatt cagggcaaac
     6181 tcaatttcta cttcactctt acccacaccg gaaaaaatcg ttttcgccgc gtcgccgcct
     6241 gccgccaaaa cgcgtgccaa ttcgccgccg gacacaatgt cgaaaccgct gcccagcgag
     6301 gcgaagtgtt tgataatgct cagattgccg tttgccttga cggcgtaaca gacgagcggg
     6361 ttcaaagcgg caaacgcggt ttggtagtgt tcaaacgctt cggtcagcgc ggattggctg
     6421 tacacataaa gcggcgtgcc gaatgcttcg gcaaggcggg ggtaggggac ttgttcgcaa
     6481 aatagggtca tattttcgtt ttcatttttg ggttggaggg gcggattgcg gtttgccttg
     6541 aagctgcaaa ccggtttgga ttacgccgaa acgcgccttg tcgccttctt tgggcaggta
     6601 gaggtcgcct ttgtaaccgc aggcggaaag caggagggcg gttgccgccg caaaaaatac
     6661 gccgtatttc atcggtaaac ttccttcata agcgcgaatg tggcaagatt cggcatctta
     6721 aacaaaaaac acgcaaaaag ccatgatgac cgaaagcgag tttatccgcg cgagcgaagc
     6781 attatttgaa cacatcgaag accaaatcga cgaaaacggc tgggatttcg actgccggtt
     6841 tgccggaaac gtcctgacca tcgaagccga aaacggcacg caaatcatcg tcaaccgcca
     6901 cacgcccaat caggaattgt ggattgccgc caaaagcggc ggctaccatt tcgccgagca
     6961 aaacggcaaa tggctggcaa cgcgagacag ccgcgatttt tatgatgttt taaacgaagc
     7021 cctgagcgcg gcttcgggcg aagcggtaga gattgaggaa ttgtgatttg ggtgttatca
     7081 cggaaagaaa aaatgaacac acgtcccttt tatttcggac ttatatttat cgcgattatt
     7141 gctatacttg ctaactattt aggaaacact gatttttccc atcattatca tatcagtgct
     7201 ttaattattg ctatcttgct gggaatggca atcggcaata ccatttatcc gcaattttca
     7261 acacaagtgg aaaaaggcgt tttgtttgcc aaaggcgcgc ttcttcgcac tggcattgtg
     7321 ctgtatggtt tccgcctcac ttttggcgat attgccgatg taggattaaa tgctgtcgtc
     7381 actgatgcaa tcatgctaat ttcaaccttc ttttttaccg tacttttagg cattcgttat
     7441 ctaaaaatgg ataaacaatt ggtttatctc actggggcag gttgcagcat ttgcggtgcg
     7501 gcagcagtga tggcggcaga gtctgttacc aaagcagaat cccataaagc ttcagtggcg
     7561 attgccatag tggtcatttt cgggacgctt gctattttta cttacccctt gttctacacg
     7621 tggtcacaag atttaattaa cgcccatcaa ttcggtattt atgttggttc tagtgtacac
     7681 gaagtggctc aagtgtatgc gattggggaa aatattgatc ctatcgtggc gaatactgcc
     7741 gtcatttcca aaatgatccg agtgatgatg ctcgccccct ttttattaat gctttcttgg
     7801 ttattaacac gtagtgatgg ggtatcagaa aatacctcac acaaaattac aattccttgg
     7861 tttgctgtac tttttattgg tgttgccatt tttaattctt ttgatttatt accaaaagaa
     7921 ctcgtgaaat tattcgttga aatcgattct ttcttattaa tttcatcaat ggctgcgctt
     7981 ggcttaacga cgcacgcaag cgcaatcaaa aaggcaggat taaaaccgtt tgttttagga
     8041 atactaactt atttatggct agtggttggt ggatttttag tgaactatgg aatatcaaaa
     8101 ttaatataaa attcactaaa gagagcgtta cccaatggca caattacccc tgtatcggac
     8161 tgccgaaatc gaaaatttta ctgtcggcac gcccgaagtt ttagaatcat tttccaaaca
     8221 tatcccttat ggtgttgtct ttgaagacga cggcgacaca ggctacttct atgccgcttc
     8281 gcaagacggg attttagatg ccttgcacat ttacaatgtc gaagatgtat ccgataaaca
     8341 tatccccaat catgtcttga ttttatggga tgatgcctgc accatagccg cattgtgtat
     8401 caacgactac attcatgccg tctatgattt tgtcgaacag gcaggatatt gccgcaacgg
     8461 cttccctgaa gcacaaggcg aatggccgaa agtcacagac cgcgtattgg acgatgaatt
     8521 gcaggacaaa atcctatccc gaaaacctac ataacctact caaaaggata acccaatgcc
     8581 cctactagac agtttcaaag tcgatcacac ccgtatgcac gccccctccg tacgcgtggc
     8641 gaaaaccatg accacgccca aaggcgacac cattaccgta ttcgacctgc gtttctgcgt
     8701 gcccaacaaa gaaatcctgc ccgaaaaagg catccacacg ctggagcatt tgttcgccgg
     8761 ttttatgcgc gaccacttga acggcaacgg cgtggaaatt atcgacattt ccccgatggg
     8821 ctgccgcacc ggtttttata tgagtttgat tggtacgcct tccgaacagc aggttgccga
     8881 tgcgtggctc gcctcgatgc aggatgtttt gaatgtcaaa gaccaaagca aaatccccga
     8941 gttgaacgag taccaatgcg gcacttatct gatgcactcg cttgccgaag cgcaacaaat
     9001 cgcgcaaaac gtgttggcgc gcaaagtggc ggtgaacaaa aatgaagagc tgatgctgga
     9061 tgaagggctg ctgaacgcct aatccgccaa aaatgccgtc tgaacaaggg tttcagacgg
     9121 catttgcctt ttccgttata atctggggtt gtgcgggggc gggttttaag ccggcatcgt
     9181 ccttccctat ttttttctgt cccttatcgg ttttaagcgg gttttttatg tccaacagac
     9241 ctacactcct ccttgttgac ggctcttcct atctctaccg cgcgtatcac gcgatggggc
     9301 aaaacctgac cgcacccgac ggtgcgccga cgggtgcgct gtatggcgta ttgaatatgt
     9361 tgcgccgttt gcggtcggaa tatccgcacg attattgcgc ggtggttttt gatgcgaaag
     9421 gcaaaaattt ccgccatcaa atgtttgaag aatacaaggc gacgcgcccg ccgatgcccg
     9481 acgatttgcg cccgcaggcg gaagcactgc cggatttagt gcgcctgacg ggctggccgg
     9541 tattggtgat tgggcaagtg gaggcggacg atgtgatcgg cacgctggcg aaacaggggg
     9601 aggaacatgg tttgcgagtc attgtttcga ccggcgataa ggacatggcg cagctggtga
     9661 acgagcgcgt gacgctggtg aacacgatga gcggcgaaac gctggacatt gaaggcgtga
     9721 aggaaaaatt cggcgtgcgt cccgaccaaa tccgcgatta tctcgcgctg atgggcgaca
     9781 aggtggacaa cgtgccgggc gtggaaaaat gcggcccgaa aacggcggtg aagtggctgg
     9841 aagcctacgg ctcgctggct ggtgtgatgg aacacgctgg cgaagtcaaa ggcaaagtgg
     9901 gcgagaatct gcgtgccgcc ctgccgcgcc taccgctgtc gtatgatttg gtaacgatta
     9961 aaaccgatgt ggatttgcac accgagcttt cagacggcat cgaaagcctg cgccgtacta
    10021 cgccgaaatg ggcgcagctg gttgtcgatt tcaaacgctg gggcttccgc acttggctga
    10081 aagaagcgga aagccgtatg cacgaagcgg tggacggcga tttgttcggc agcgacagca
    10141 tcggcgagca ggcggctttg gatgtggaaa tgccgtctga aagactgtct gaaaaagccg
    10201 ccgtccccga aaagctggat tatcaagccg ttactaccga agcgcagttt gccgccttgt
    10261 tggacaaact gtcgcaggcg gacaaaatcg gcatcgacac ggaaaccaca tccctagacg
    10321 cgatgaacgc cgcgctggtc ggcatcagca tcgcgttcca agcaggggag gcggtttaca
    10381 tccccgtagg acacagcctg accgccgcgc ccgaacagct tgatttgcaa gacgtattag
    10441 gccgtctgaa accgcatttg gaaaatcccg ccctgaaaaa aatcgggcaa aacctcaaat
    10501 acgaccaaca tgtttttgcc aactacgaca tcgccctgaa cggcattgcc ggcgacgcca
    10561 tgctcgcttc ctacatcatc gagagccatc tcggacacgg tttggacgaa ttgtccgaac
    10621 gctggctggg cttggaaacc attacctacg aatcgctgtg cggcaaaggc gcgaagcaaa
    10681 tcggttttgc cgacgtcgct atcgggcagg cgaccgaata cgccgcccaa gacgccgatt
    10741 tcgccctgcg cctcgaagcg catctgcgcg cgcaaatgga cgaaaaacag cttgaaatgt
    10801 atgaaaaaat ggagctgccc gtcgcgcagg tattgtttga aatggaacgc aacggcgtgc
    10861 aaatcgaccg cgccgaactc gcccgccaaa gcgcggaact cggcgccgag ctgatgaagc
    10921 tcgaacaaca agcctatgcc gccgcaggcc agccgttcaa cctcaattcg cccaaacagc
    10981 tgcaagaaat cctgttcgac aaaatgggca tccccaccaa aggcctgaaa aaaaccgcca
    11041 aaggcggcat ttccaccaac gaagccgtgc tcgaacagct cgcgcccgac taccccctgc
    11101 ctaaaatcat cctgcaaaac cgcagcctgg cgaagctcaa atccacctac accgacaaac
    11161 tacccgaaat gatttccccc aaggacggcc gcgtgcatac cacctacgcc caagccgtcg
    11221 ccattacagg acgcttggca agcaacaacc ccaacctgca aaacatcccc atccgcaccg
    11281 ccgaaggccg acgcgtgcgc cgcgccttca ccgcaccgca aggcagcgtc atcgtttccg
    11341 ccgactattc ccaaatcgag ctgcgcatca tggcgcacct ctccggcgat aaaaccctca
    11401 ttgccgcgtt ccaaaacggc gaagacgtac accgccgcac cgccgccgaa gtgttcggca
    11461 tcgcccccga aaacgtctcg tccgaacaac gccgctacgc caaaaccatc aacttcggcc
    11521 tgatttacgg catgggccaa tacggccttg ccaaatcatt gggcatcgac aacatctccg
    11581 ctaaaaactt tatcgaccgc tacttcgccc gctaccccgg cgtcgccgaa tacatgcagc
    11641 gcaccaaaga acaagccgcc gcccaaggct acgtcgaaac cctgttcggc agaaggctct
    11701 atttgcccga catccgcaac aaaaacgcca acgcccgcgc aggagccgaa cgcgccgcca
    11761 tcaacgctcc catgcaaggc accgcgtccg acctcatcaa acgcgccatg atagacgtgc
    11821 gaaactggct ttcagacggc atcggcagca aactggtcat gcaggtgcat gacgaactgg
    11881 tgctggaagt cgttgaaacc gaactggatt ttgtcaaaga aaaactgccg cagattatgg
    11941 cgaaagtgga cggcggatta ttggatgtac cgctggtggc tgaggttggc gtaggggaga
    12001 attgggaaga ggcacattga ttgaaaggtg ttatatgcta tctttattta aataaaattt
    12061 aatttttggt atattttttc taaatgttcc tatagtatag tggat
//
LOCUS       DAWFHY010000063        12089 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.063, whole genome shotgun sequence.
ACCESSION   DAWFHY010000063 DAWFHY010000000
VERSION     DAWFHY010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12089
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.063"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(405..1430)
                     /locus_tag="WGQ68_001503"
     CDS             complement(405..1430)
                     /locus_tag="WGQ68_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEZ0715282.1"
                     /translation="MKIKALMIAAALLAAADVHAAPQKAKTASAKAAKAAKVAKVAKV
                     AATAQKEAAPAQQQGGIRFSDGIAAVADNEVITRRRLAEAVAEAKANLPKDAQISESE
                     LSRQVLMQLVNQSLIVQAGKRRNIQASEAEIDAVVAKNPALKNLSPAQRRDFADNIIA
                     EKVRQQAVMQNSRVSEAEIDAFLEQAQKQGITLPEGAPLRQYRAQHILIKADSENAAV
                     GAESTIRKIYGEARSGTDFSSLARQYSQDASAGNGGDLGWFADGVMVPAFEEAVHALK
                     PGQVGAPVRTQFGWHIIKLNEVRDAGTPQERIRNSVRQYIFQQKAEQATVNLLRDLHS
                     GAYVDIR"
     gene            complement(1535..3943)
                     /locus_tag="WGQ68_001504"
     CDS             complement(1535..3943)
                     /locus_tag="WGQ68_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HEZ0715283.1"
                     /translation="MARLFSLKPLVLALGLCFGTHCAAADAVAAEETDNPTAGGSVRS
                     VSEPIQPTSLSLGSTCLFCSNESGSPERTEAAVQGSGEASIPEDYTRIVADRMEGQSQ
                     VQVRAEGNVVVERNRTTLNADWADYDQSGDTVTAGDRFALQQDGTLIRGETLTYNLEQ
                     QTGEAHNVRMETEHGGRRLQSVSRTAEMLGEGHYKLTETQFNTCSAGDAGWYVKAASV
                     EADREKGIGVAKHAAFVFGGVPIFYTPWADFPLDGNRKSGLLVPSLSAGSDGVSLSVP
                     YYFNLAPNLDATFAPGVIGERGAVFDGQVRYLRPDYAGQSDLTWLPHDKKSGRNNRYQ
                     AKWQHRHDISDTLQAGVDFNQVSDSGYYRDFYGNKEIAGNVNLNRRVWLDYGGRAAGG
                     SLNAGLSVLKYQTLANQSGYKDKPYALMPRLSADWRKNTGRAQIGVSAQFTRFSHDSR
                     QDGSRLVVYPDIKWDFSNSWGYVRPKLGLHATYYSLNRFGSQEARRVSRTLPIVNIDS
                     GMTFERNTRMFGGGVLQTLEPRLFYNYIPAKSQNDLPNFDSSESSFGYGQLFRENLYY
                     GNDRINTANSLSAAVQSRILDGATGEERFRAGIGQKFYFKNDAVMLDGSVGKKPRSRS
                     DWVAFASSGIGSRFILDSSIHYNQNDKRAENYAVGASYRPAQGKVLNARYKYGRNEKI
                     YLKSDGSYFYDKLSQLDLSAQWPLTRNLSAVVRYNYGFEAKKPIEVLAGAEYKSSCGC
                     WGAGVYAQRYVTGENTYKNAVFFSLQLKDLSSVGRNPADRMDVAVPGYIPAHSLSAGR
                     NKRP"
     gene            4013..5017
                     /locus_tag="WGQ68_001505"
     CDS             4013..5017
                     /locus_tag="WGQ68_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEZ0715284.1"
                     /translation="MQRQIKLKNWLQTVYPERDFDLTFAAADADFRRYFRATFSDGGS
                     VVCMDAPPDKMSVAPYLKVQKLFDMVNVPQVLHADTDLGFVVLNDLGNTTFLTAMLQE
                     QGEAAHKALLLEAIGELVELQKASREGVLPEYDRETMLREINLFPEWFVAKELGRELT
                     FKQRQLWQQTVDTLLPPLLAQPKVYVHRDFIVRNLMLTRGRPGVLDFQDALYGPISYD
                     LVSLLRDAFIEWEEEFVLDLVIRYWEKARADGLPVPEAFDEFYRWFEWMGVQRHLKVA
                     GIFARLYYRDGKDKYRPEIPRFLNYLRRVSRRYAELAPLYALLVELVGDEELETGFTF
                     "
     gene            complement(5074..5772)
                     /locus_tag="WGQ68_001506"
     CDS             complement(5074..5772)
                     /locus_tag="WGQ68_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HEZ0715285.1"
                     /translation="MKSRHLALGVAALFALAACDSKVQTSVPADSAPAASAAAAPAGL
                     VEGQNYTVLANPIPQQQAGKVEVLEFFGYFCPHCAHLEPVLSKHAKSFKDDMYLRIEH
                     VVWQKEMLPLARLAAAVDMAAADSKDVANSHIFDAMVNQKIKLQNPEVLKKWLGEQTA
                     FDGKKVLAAYESPESQARADKMQELTETFQIDGTPTVIVGGKYKVEFADWESGMNTID
                     LLADKVREEQKAAQ"
     gene            complement(6052..6159)
                     /locus_tag="WGQ68_001507"
                     /pseudo
     CDS             complement(6052..6159)
                     /locus_tag="WGQ68_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            6217..7755
                     /gene="murJ"
                     /locus_tag="WGQ68_001508"
     CDS             6217..7755
                     /gene="murJ"
                     /locus_tag="WGQ68_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HEZ0715286.1"
                     /translation="MNMLGALAKVGSLTMVSRVLGFVRDTVIARAFGAGMATDAFFVA
                     FKLPNLLRRVFAEGAFAQAFVPILAEYKETRSKEATEAFIRHVAGMLSFVLVIVTALG
                     ILAAPWVIYVSAPGFAKDADKFQLSIDLLRITFPYILLISLSSFVGSVLNSYHKFSIP
                     AFTPTFLNVSFIVFALFFVPYFDPPVTALAWAVFVGGILQLGFQLPWLAKLGFLKLPK
                     LNFKDAAVNRVMKQMAPAILGVSVAQISLVINTIFASYLQSGSVSWMYYADRMMELPS
                     GVLGAALGTILLPTLSKHSANQDTEQFSALLDWGLRLCMLLTLPAAVGLAVLSFPLVA
                     TLFMYREFTLFDAQMTQHALIAYSFGLIGLIMIKVLAPGFYARQNIKTPVKIAIFTLI
                     CTQLMNLAFIGPLKHVGLSLAIGLGACINAGLLFYLLRRHGIYQPGKGWAAFLAKMLL
                     SLAVMGGGLYAAQIWLPFDWAHAGGMQKAARLFILIVIGGCLYFASLAALGFRPRHFK
                     RVES"
     gene            7888..8844
                     /locus_tag="WGQ68_001509"
     CDS             7888..8844
                     /locus_tag="WGQ68_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheT family hydrolase"
                     /protein_id="HEZ0715287.1"
                     /translation="MILTPPDTPLFLRNGNADTIAAKFLQRPAPAYRRELLPDSTGKT
                     KVAYDFSDGISPDAPLVVLFHGLEGSSRSHYAVELMLAVRDRGWHGVVVHFRSCGGIA
                     NTAPVFYHLGDTAEIAFTLDTFAARYREIYAVGVSLGGNALAKYLGEQGENALPQAAA
                     VISAPVDAEAAGNRFDSGITRLLYTRYFLRTLIPKARSLQGFQTAFAAGCKTLGEFDD
                     RFTAPLHGFADRHDYYRQTSCKPLLKHVAKPLLLLNAVNDPFLPPEALPTPDEVSETV
                     TLFQPAHGGHVGFVSSTGGRLHLQWLPQTVLSYFDSFRTNRR"
     gene            8894..9676
                     /gene="trpC"
                     /locus_tag="WGQ68_001510"
     CDS             8894..9676
                     /gene="trpC"
                     /locus_tag="WGQ68_001510"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEZ0715288.1"
                     /translation="MTDILNKILATKAQEVAAQKAAVNAEHIRALAAEAAPVRSFIDL
                     IRGKHRLNLPAVIAEIKKASPSKGLIRPDFRPAEIARAYENAGAACLSVLTDEPYFQG
                     SPEYLKQAREAVSLPVLRKDFIIDEYQVYQARAWGADAVLLIAAALEQEQLERFEAVA
                     HELGMTVLLELHDETELEKCRNLTTPLWGVNNRNLRTFEVSLDQTLSLLPALEGKTVV
                     TESGITGKADVEFMRTRGVHTFLIGETFMRAADIEAEVGKLF"
     gene            9741..12041
                     /gene="recQ"
                     /locus_tag="WGQ68_001511"
     CDS             9741..12041
                     /gene="recQ"
                     /locus_tag="WGQ68_001511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEZ0715289.1"
                     /translation="MHRPTAKQILHEVFGYPEFRGKQESIVNALAGGESLMVLMPTGG
                     GKSLCYQIPALMREGVAVVVSPLIALMNDQVASLHVAGIEAAAVNSSTSADEAREIAD
                     KLAQGRLKLLYVAPERLVTDRFLRFLDQQTVSLFAIDEAHCVSQWGHDFRPEYQQLGI
                     LAERYPNVPRIALTATADAATRADIKHYLHLDNAPEFVSSFDRPNIYYQVIEKNNGKK
                     QLLDFIRKEMTGQSGIVYCLSRKKVEDVAQFLRENGLNAIPYHAGLSMDVREENQRRF
                     THEDNIIVVATVAFGMGIDKPDVRFVAHLDMPQSVEHFYQESGRAGRDGLPAVSWLCY
                     GLNDWVLLRERIAEGNSDEVQKQIEMQKLDAMLSVCETAACRRVLLLKHFGEASEPCG
                     HCDNCLHPPVRFDGTVLVQKLLSCVYRAGQRFAAGYITNLLRGKSDDWIRGNRHEQLS
                     TFGIGTELSDKEWRSVIRQCISLGYLTVNITRYQALQLTEAAKKVLKGETEVMLRPLK
                     RDKPAARTLKDNWLRTEREERLWQALRVWRMKQAEAEVIPAYMIFGDKTLRDLVEKMP
                     QDLNGLHDIYGLGEAKIDRFGHGILEVCRNTADFSHNAVIRPQTEREQQLRQKLEAWR
                     YEQARAENCALHAVLSDESLADMLADTPETETDLEGVHGLGSVRAAKYGRDILAVCRP
                     FSDGIDEAAKHKRRLMRALIQWCNETAKHEQSEPYRILSKAALRAIAAKQPEGLAELA
                     AVYGVGEEKAARYGAAVLAVLERNAV"
BASE COUNT         2451 a         3369 c         3458 g         2811 t
ORIGIN      
        1 ttcgcgggaa tgacggcata gaggtttctg ttttcccgat aaattcctgt tacttttcgt
       61 tgctagattc ccgcctacgc gggaatgacg atggaaagat tgttgttgct tcggataaat
      121 ttctgcagtt ttaaataacc ggattcccgc ctgctcggga atgacggcat aggttttttt
      181 gtttttccga taaattacca caacccaaaa tcccgtcatt cccgcgaaag cgggaatcca
      241 gacctttaaa ttccagccat tcccgataaa ttcctgttac ttttcgtttc tagattcccg
      301 ctttcgcggg aatgatgaat gacggtgtaa aagtaactcg aaatccaaaa agccataccg
      361 cccggatttt tgccgatcgg tatggctttt tgcttcaaac cgccttagcg gatgtcgaca
      421 tacgcgccgg aatgcaggtc acgcaacagg ttgacggttg cctgttcggc tttttgttgg
      481 aagatgtatt gccgcacgga attgcggata cgttcctgag gtgtgccggc atcgcgcact
      541 tcgttcaatt tgatgatatg ccagccgaat tgggtgcgga cgggcgcgcc gacctgtccg
      601 ggtttgagcg cgtggacggc ttcttcaaag gcgggaacca tcacgccgtc ggcaaaccag
      661 cccaaatctc cgccgttgcc cgcgctcgcg tcttgcgaat attggcgcgc taggctggaa
      721 aagtctgtgc cgctgcgggc ctctccgtag attttgcgga tggtgctttc cgcgccgacg
      781 gcagcgtttt cgctgtcggc tttaatcagg atgtgttggg cgcggtattg gcgcaacggt
      841 gcgccttcgg gcagggtgat gccttgtttt tgcgcctgct cgaggaaggc atcgatttca
      901 gcttcgctca ctcggctgtt ctgcatcact gcctgctggc ggactttttc ggcaatgatg
      961 ttgtcggcaa aatcgcggcg ttgggcgggg ctgaggtttt tgagggcggg attttttgcg
     1021 acgacggcat cgatttccgc ttcgcttgct tgaatgttgc ggcgtttgcc cgcctgtaca
     1081 atcagggatt ggttgacaag ctgcatcagc acctgtcggg acagctcgga ttcgcttatc
     1141 tgcgcgtctt tgggcaggtt ggctttggct tcggcaacgg cttcggcaag ccggcggcgc
     1201 gtgatgactt cgttgtcggc aacggcggca atgccgtctg aaaagcggat accgccctgc
     1261 tgttgtgcgg gtgcggcttc tttttgcgcc gtggcggcaa ctttggcaac tttggcaact
     1321 ttggcagctt tggcagcttt ggcggatgcg gtttttgcct tttgcggtgc ggcgtggaca
     1381 tcggctgctg ccagcaatgc ggcggcaatc atcagggctt tgattttcat cattctttcc
     1441 ttatcgtttg aatcctgcct ttgatggcgg gtttggggtt gtcggtggaa tgccgtcttg
     1501 tgcgggaacg gtgctcctgc cgggtttccg acggtcaggg ccgtttgttg cgtccggcgg
     1561 aaagagagtg ggcggggata tagccgggaa cggcgacatc catcctgtct gcggggtttc
     1621 tgccgacact gctgaggtct ttcaactgaa gtgagaaaaa gacagcgttt ttgtaggtgt
     1681 tttcgccggt aacgtagcgt tgggcgtaca cgcccgcgcc ccagcagccg caactgcttt
     1741 tgtattccgc acccgccagc acctctatcg gttttttggc ttcaaaaccg tagttgtaac
     1801 ggacgacggc cgacaggttg cgcgtcagcg gccattgtgc ggacaggtcg agctggctga
     1861 gtttgtcgta aaaataggaa ccgtcggact tcaggtagat tttttcgttg cgcccgtatt
     1921 tgtagcgggc gttcagcact ttgccctgtg cgggacggta gcttgcaccg acggcgtagt
     1981 tctcggcgcg tttgtcgttt tggttgtagt ggatgctgct gtcgaggatg aagcggctgc
     2041 cgatgccgct ggaggcgaat gccacccagt cggaacggct gcgcggtttt ttgccgacac
     2101 tgccgtcaag catgactgcg tcgtttttga agtagaattt ctgcccgatg ccggcgcgga
     2161 aacgctcttc ccccgtcgcg ccgtccaaaa tacggctttg cacggcggcg gaaaggctgt
     2221 ttgcggtgtt aatcctgtcg ttgccgtaat agaggttttc acgaaaaagc tgcccgtagc
     2281 cgaagctgct ttccgacgaa tcgaaattgg gcaggtcgtt ttgggatttg gcaggaatat
     2341 agttgtagaa caggcgcggc tcgagggttt gcaggactcc gccgccgaac atccgcgtat
     2401 tgcgttcgaa ggtcatgccg ctgtcgatgt tgacgatggg cagagtgcgg ctgacgcgtc
     2461 gggcttcttg gctgccgaag cggttgaggc tgtaataggt ggcgtgcagt ccgagtttgg
     2521 gacggacgta accccagctg ttgctgaaat cccatttgat gtcgggatag acgacgaggc
     2581 ggctgccgtc ttggcggctg tcgtggctga agcgggtaaa ttgggcggac acgccgattt
     2641 gcgccctgcc ggtgtttttg cgccaatcgg cggaaaggcg cggcatcagg gcatacggtt
     2701 tgtctttgta gccgctttgg tttgccagcg tctggtattt cagaaccgaa aggccggcat
     2761 tcaggctgcc gcccgccgcc ctgccgccat aatccagcca tacacggcgg ttgaggttga
     2821 cgttgccggc gatttctttg ttgccgtaaa agtcgcggta gtagccgctg tcggagactt
     2881 ggttgaaatc gacacccgcc tgaagcgtgt cggaaatgtc gtgccggtgc tgccatttcg
     2941 cctgatagcg gttattcctg ccgcttttct tgtcgtgcgg cagccaggtc aggtcggact
     3001 ggccggcata atccggccgc aggtagcgta cctgcccgtc aaagaccgcg ccgcgttcgc
     3061 cgatcacgcc gggcgcgaac gtggcatcga gattgggggc aaggttgaaa taatagggaa
     3121 cggaaaggga aacgccgtcc gaaccggcgg acagtgaggg aacgagcagg ccgcttttgc
     3181 ggttgccgtc aagcgggaag tccgcccaag gggtgtagaa aatgggaacg ccgccgaaca
     3241 cgaaggcggc gtgtttggca acgcctatgc ctttttcccg atcggcttcg acggaagcgg
     3301 ccttgacata ccagccggca tcgccggcgg aacaggtgtt gaattgggtt tccgtcagtt
     3361 tgtaatgccc ttcgcccaac atttcggcgg tgcggctgac gctttgcagc cgccgtccgc
     3421 cgtgttcggt ttccatacgg acgttgtgcg cttccccggt ctgctgctcg agattgtagg
     3481 tcagggtttc gccccgaatc agcgtaccgt cctgttggag ggcgaaccgg tcgcctgcgg
     3541 taacggtgtc gcccgactgg tcgtaatccg cccaatcggc attgagggtc gtccgattgc
     3601 gttcgacgac gacgttgcct tcggcacgca cctgcacctg cgactgtcct tccatcctgt
     3661 cggcaacaat gcgcgtatag tcttcgggga tggatgcttc gccgctgcct tggacggcgg
     3721 cttcggttct ctcggggctg ccgctttcgt tactgcaaaa caggcaggtc gaaccgaggc
     3781 tcaggctggt aggctgtatg ggttcggaca cgctccgaac gcttcctccg gcggtcggat
     3841 tgtccgtttc ctccgccgca acggcatcgg cggcggcgca atgcgtgccg aagcagaggc
     3901 ccaatgccag caccagtggt ttgagtgaaa ataaacgagc caaaatcgcc cctcaagtcg
     3961 gtttgccagt tagaatagcg tttattgtaa cctgaaatgc tttagtactg ttatgcaacg
     4021 gcaaatcaaa ctgaaaaatt ggcttcagac cgtttatccc gaacgggact tcgatctgac
     4081 ttttgcggcg gcggatgctg atttccgccg ctatttccgt gcaacgtttt cagacggcgg
     4141 cagtgtcgtc tgcatggatg caccgcccga caagatgagt gtcgcacctt atttgaaagt
     4201 gcagaaactg tttgacatgg tcaatgtacc gcaggtattg cacgcggaca cggatttggg
     4261 gtttgtggta ttgaacgact tgggcaatac gacgtttttg accgcgatgc ttcaggagca
     4321 gggcgaagcg gcgcacaaag ccctgctttt ggaggcaatc ggcgagctgg tcgaattgca
     4381 gaaggcaagc cgtgaagggg ttttgcccga atatgaccgt gaaacgatgc tgcgcgaaat
     4441 caacctgttc ccggaatggt ttgtcgccaa agaattgggg cgcgaattaa cattcaaaca
     4501 acgccaactt tggcagcaaa ccgtcgatac gctgctgccg cccctgttgg cgcagcccaa
     4561 agtctatgtg caccgcgact ttatcgtccg caacctgatg ctgacgcgcg gcagaccggg
     4621 cgttttagac ttccaagacg cgctttacgg cccgatttcc tacgatttgg tgtcgctgtt
     4681 gcgcgatgcc tttatcgaat gggaagaaga atttgtcttg gacttggtta tccgctactg
     4741 ggaaaaggcg cgggctgacg gcttgcccgt ccccgaagcg tttgacgagt tttaccgctg
     4801 gttcgaatgg atgggcgtgc agcggcactt gaaggttgca ggcatcttcg cacgcctgta
     4861 ctaccgtgac ggcaaagaca aataccgtcc cgaaatcccg cgtttcctca attacctgcg
     4921 ccgcgtatcg cgccgttatg ccgaactcgc cccgctctac gcgctcttgg tcgaactggt
     4981 cggcgatgaa gaactggaaa cgggctttac gttttaaacc caatcaaaat gccgtctgaa
     5041 aaccaagttt cagacggcat ttttcaaacg ggcttactgc gcggcttttt gttcttcgcg
     5101 tactttgtcc gccaaaaggt cgatggtgtt cataccggac tcccagtcgg caaattcaac
     5161 tttatattta ccgccgacga taaccgtggg cgtaccgtcg atttggaagg tttcggtcag
     5221 ctcctgcatt ttgtcggcgc gcgcctggct ttcgggggac tcgtaggcgg caaggacttt
     5281 tttgccgtca aaggcggttt gttcgcccag ccattttttg aggacttccg gattttgcag
     5341 cttgattttt tggttgacca tcgcatcgaa aatatggctg ttcgccacat ctttgctgtc
     5401 ggcggcagcc atatcgacgg cggcggcgag gcgtgccagc ggcagcattt ctttctgcca
     5461 gacgacgtgc tcgatacgca ggtacatatc gtctttaaaa gacttggcgt gtttgctcaa
     5521 aacaggttcg aggtgggcgc agtgcgggca gaaatagccg aaaaactcaa ggacttcaac
     5581 cttgcctgcc tgctgttggg gaatcgggtt ggcaaggacg gtatagtttt gcccttcgac
     5641 cagtccggcg ggggcggcgg ctgccgaagc agcaggcgcg ctgtcggcgg ggacgctggt
     5701 ttggactttg ctgtcgcacg cggcaagggc gaacagggcg gcaacgccga gggcgaggtg
     5761 tctggatttc atacggctct ccgtgatgtt ggaaataaat cggatattgg tattttattg
     5821 catttccgcg tgttgataca gtttgccgcc ggaaaaggac gttttcgttt ggggaaaccg
     5881 cttcggacgg catcaaaccc gatgccgtct gaagcggttt ctgtcgtaca atacgcgccg
     5941 tcgccccaga cggatacggc tgttggggaa ctaatgatgt tccaacacac aggacggcac
     6001 ataaagcacc gccctatgcg ttgccctggt tcggaagggt tacacccctt tcaaatatag
     6061 tggattaaat ttaaaccagt acagcgttgc ctcgccttgc cgtactattt gtactgtctg
     6121 cggcttcgtc gccttgtcct gatttaaatt taatccacta tcaaagtctg atcctgccgc
     6181 cctaaagggc ggggtttcaa ccgaaaagga aatacgatga atatgctggg agctttggca
     6241 aaagtcggca gcctgacgat ggtgtcgcgc gttttgggat ttgtgcgcga tacggtcatt
     6301 gcgcgcgcat tcggcgcggg catggcgacg gatgcgtttt ttgtcgcgtt caaactgccc
     6361 aacctgcttc gccgcgtgtt tgcggagggg gcgtttgccc aagcgtttgt gccgattttg
     6421 gcggaatata aggaaacgcg ttctaaagag gcgacggagg cttttatccg ccatgtggcg
     6481 gggatgctgt cgtttgtgct ggtcatcgtt accgcgctgg gcatacttgc cgcgccttgg
     6541 gtgatttatg tttccgcacc cggttttgcc aaagatgccg acaaatttca gctctccatc
     6601 gatttgctgc ggattacgtt cccctatatc ttattgattt cactttcctc ttttgtcggc
     6661 tcggtactca attcctatca taaattcagc attccggcgt ttacgcccac gttcctgaac
     6721 gtgtcgttta tcgtattcgc gctgtttttc gtgccgtatt tcgatcctcc cgttaccgcg
     6781 ctggcttggg cggtttttgt cggcggtatt ttgcagctcg gctttcagct cccttggttg
     6841 gcgaaactgg gttttttgaa actgcccaaa ctgaatttca aagatgcggc ggtcaaccgc
     6901 gtcatgaaac aaatggcgcc tgcgattttg ggcgtgagcg tggcgcagat ttctttggtg
     6961 attaacacga ttttcgcgtc ttatctgcaa tcgggcagcg tttcatggat gtattacgcc
     7021 gaccgcatga tggagctgcc cagcggcgtg ctgggggcgg cactcggtac gattttgctg
     7081 ccgactttgt ccaaacactc ggcaaaccaa gatacggaac agttttccgc cctgctcgac
     7141 tggggtttgc gcctgtgcat gctgctgacg ctgccggcgg cggtcggact ggcggtgttg
     7201 tcgttcccgc tggtggcaac cttgttcatg taccgagaat tcacgctgtt tgacgcgcag
     7261 atgacgcaac acgcgctgat tgcctattct tttggtttaa tcggtttaat catgattaaa
     7321 gtgttggcac ccggcttcta tgcgcggcaa aacatcaaaa cgcccgtcaa aatcgccatc
     7381 ttcacgctca tctgcacaca gttgatgaac cttgccttta tcggcccact gaaacacgtc
     7441 ggactttcgc ttgccatcgg tctgggcgcg tgtatcaatg ccggattgtt gttttacctg
     7501 ttgcgcagac acggtattta ccaacctggc aagggttggg cagcgttctt agcaaaaatg
     7561 ctgctctcgc tcgccgtgat gggaggcggc ctgtatgccg cccaaatctg gctgccgttc
     7621 gactgggcac acgccggcgg aatgcaaaag gccgcccggc tcttcatcct gattgtcatc
     7681 ggaggctgcc tgtatttcgc atcactggct gcactgggct tccgtccgcg ccatttcaaa
     7741 cgggtggaaa gctgaccgat gccgatatcc tttttcggca aacgcttttt ccacgcctat
     7801 attcgggcgc gcatcccccg cgtccgcacc ggccggacgg gctgccgcat catcaatgcc
     7861 gtctgaaaac aagaaaaccg ataccttatg attttgacac cgccggacac gccccttttc
     7921 ctccgcaacg gcaatgccga cacgattgcc gccaaattcc tgcaacgccc cgcacctgca
     7981 taccgccgcg agctgcttcc cgacagcacg ggtaaaacca aagtcgccta cgacttttca
     8041 gacggcattt cgcccgatgc gccgctggtc gtgctgtttc acggtttgga aggaagcagc
     8101 cgcagccatt acgcggtcga actgatgctt gcggtacgcg atcggggttg gcacggcgta
     8161 gtcgtccatt tccgcagctg cggcggcatt gccaacaccg ctccggtgtt ctaccacttg
     8221 ggcgataccg ccgaaatcgc ctttactttg gacacgttcg ccgcgcgtta ccgtgaaata
     8281 tacgccgtcg gcgtatcgct gggcggcaac gcgctggcaa aatatttggg cgaacagggc
     8341 gaaaacgcgc tgccgcaagc cgccgccgtc atctccgcac ccgtcgatgc agaggcggca
     8401 ggcaaccgct tcgacagcgg catcacacgg ctgctctaca cgcgctactt cctccgcaca
     8461 ctgataccca aagcacggtc gctccaaggt tttcagacgg catttgccgc agggtgcaaa
     8521 acactgggcg agtttgacga ccgtttcacc gcaccgctgc acggctttgc cgaccggcac
     8581 gactactacc gccaaacttc ctgcaaaccg ctgctcaaac acgttgccaa accgctgctc
     8641 ctgctcaatg ccgtcaacga ccccttcctg ccgcccgaag cgctgccgac accggacgaa
     8701 gtgtccgaaa ccgttaccct gttccaacct gcacacggtg gtcatgtcgg ctttgtcagc
     8761 agcaccggcg gcaggctgca cctgcaatgg ttgccgcaga ccgtcctgtc ctatttcgac
     8821 agcttccgca caaacaggcg ttaacggttt gatgctaata ttcccccttt cccagacgaa
     8881 tacggaacac gacatgaccg acatcctcaa taaaatcctt gccaccaaag cacaggaagt
     8941 tgccgcccaa aaagccgccg tcaacgccga acacatccgc gcacttgccg cagaagccgc
     9001 gcccgtccgc agcttcatcg atttgatacg cggcaaacac cgcctaaacc tgcccgccgt
     9061 cattgccgaa atcaaaaagg caagcccgag caaaggcctg atccgtccgg acttccgacc
     9121 ggccgagatt gcacgcgcat atgaaaacgc cggagcggca tgtttgtccg tactgaccga
     9181 cgaaccctat ttccaaggtt cgcccgaata cctcaaacag gcgcgcgaag ccgtatcgct
     9241 gcccgtgctg cgcaaagact tcatcatcga cgaatatcag gtttatcagg cgcgcgcatg
     9301 gggggcggat gccgtcctgc tgattgccgc agcactggaa caggaacaat tggaacgctt
     9361 tgaagcggtg gcgcacgaat tgggcatgac cgtcctgctc gagctgcacg atgaaaccga
     9421 attggaaaaa tgccgcaacc tgaccacgcc gttgtggggc gtaaacaacc gcaacctgag
     9481 gacttttgaa gtctccctcg accaaaccct gtcgctgctg cccgcgctgg aaggcaaaac
     9541 cgtcgttacc gaaagcggca ttacgggtaa ggcggatgtg gaatttatgc ggacgcgcgg
     9601 tgtgcacacc ttcctgatcg gcgaaacgtt catgcgtgcc gccgatattg aggcggaagt
     9661 gggcaaactc ttctaaatcc cgatttcaga cggcatattg ccgccgaccg atcaacaccc
     9721 ttaccgagca cggaaccatc atgcaccgac cgaccgccaa acagattttg cacgaagtat
     9781 tcggttatcc cgaatttcgc ggcaagcagg agtctatcgt caatgctttg gccggcggcg
     9841 agagtttgat ggtgctgatg ccgacgggcg ggggcaagtc tttgtgttac cagattccgg
     9901 cgctgatgcg cgaaggcgtg gcagttgtcg tatcgccgct gattgcgttg atgaacgacc
     9961 aagtggccag tctgcatgtg gccgggattg aagcggcggc agtcaacagc agcacatcgg
    10021 cagatgaggc gcgcgagatt gccgacaagc ttgcccaagg ccgtctgaaa ctgctttatg
    10081 tcgcgccgga acgcttggtt accgaccgct ttttgcgttt tctcgaccaa caaaccgtca
    10141 gcctgtttgc cattgacgag gcgcattgcg tcagccaatg gggacacgat ttccgccctg
    10201 aatatcaaca gctcggcatc cttgccgaac gctatccgaa cgtcccgcgc atcgccctga
    10261 ccgctaccgc cgatgccgcc acgcgcgccg acatcaagca ttatctgcac ttggacaatg
    10321 cgcccgaatt tgtctccagc ttcgaccgtc cgaatattta ttatcaggtt atcgaaaaaa
    10381 acaacggcaa aaaacaattg ctggatttca tccgcaaaga aatgacgggg caaagcggca
    10441 ttgtgtattg cctaagccgc aaaaaggttg aagatgtggc gcagtttttg cgtgaaaacg
    10501 gattaaacgc gattccgtat catgctggtt tgagcatgga cgtgcgcgag gaaaaccaac
    10561 gccgctttac gcatgaagac aatattatcg tggtggcgac cgtggcgttc ggcatgggca
    10621 tagacaaacc cgacgtgcgc tttgtcgccc atctcgatat gccccagagt gtcgaacatt
    10681 tctatcagga atcggggcgt gccggccggg acgggctgcc tgccgtgagc tggctgtgtt
    10741 acggtttgaa cgattgggtg ttgctgcgcg aacggattgc cgaaggcaac agcgacgagg
    10801 tgcaaaagca aatcgaaatg caaaaactcg atgccatgct ttccgtctgc gaaaccgccg
    10861 cctgccgccg cgtactgctg ctcaaacatt tcggcgaagc atccgaaccc tgcggccatt
    10921 gcgacaactg cctgcatccg cccgtacggt ttgacggcac ggtgttggtg caaaaattac
    10981 tcagctgcgt gtaccgcgcc ggacaacgtt ttgccgccgg ttacatcacc aaccttttgc
    11041 gcggcaaaag cgacgattgg atacgcggca accggcacga acaactgtcc accttcggca
    11101 tcggtacgga gttgtccgac aaagaatggc gcagcgtcat ccgccagtgt atcagcctcg
    11161 gctacctcac cgtcaacatt acccgatatc aggcattgca actgaccgaa gccgccaaaa
    11221 aagtcctcaa aggcgaaacc gaagtgatgc tgcgtccgct caagcgcgac aagcccgccg
    11281 cccgcaccct caaagacaac tggctgcgta ccgaacgcga agaacgcctg tggcaggcat
    11341 tgcgcgtttg gcgtatgaaa caggcagaag ccgaagtcat ccccgcctat atgattttcg
    11401 gcgacaaaac cctgcgcgac cttgtcgaaa aaatgccgca agacctcaac ggactgcacg
    11461 acatctacgg tttgggcgaa gccaaaatcg accgtttcgg acacggcatc ctcgaagtct
    11521 gccgcaacac cgccgacttt agccacaatg ccgtcatccg cccgcaaacc gaacgcgaac
    11581 aacaactgcg tcaaaaactc gaagcctggc ggtatgaaca ggcaagggcg gaaaactgcg
    11641 ccctgcatgc cgtcctctcc gacgaaagcc ttgccgatat gcttgccgat acgcccgaaa
    11701 ccgaaaccga cctcgaaggc gtacacggct tgggcagcgt acgcgccgcc aaatacggac
    11761 gggacatcct cgccgtctgc cgtccgtttt cagacggcat cgatgaagcc gccaaacaca
    11821 aacgccgcct gatgcgcgcc ctgattcaat ggtgcaacga aacggcaaaa cacgaacagt
    11881 ccgaacccta ccgcattctc agcaaagccg ccctgcgcgc cattgccgcc aaacagccgg
    11941 aaggtttggc ggagcttgcc gccgtatacg gcgtaggcga agaaaaagcc gcacgttacg
    12001 gtgcggcggt gttggcggta ttggaacgaa atgccgtctg aaaaccgtta ccaagtttca
    12061 gacggcattg cctctattta aaaattcct
//
LOCUS       DAWFHY010000064        12017 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.064, whole genome shotgun sequence.
ACCESSION   DAWFHY010000064 DAWFHY010000000
VERSION     DAWFHY010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 12017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12017
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.064"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..332)
                     /locus_tag="WGQ68_001512"
     CDS             complement(<1..332)
                     /locus_tag="WGQ68_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEZ0715290.1"
                     /translation="MFSLYREDSLISRLNRDGYLTSPSADFLELLSLCRDIHALTDGA
                     FDPTVQALWKLYADYFAARPDAETPPPQNSVDEALKRVGFGKVSFDDREIRFAEKGMG
                     LSLNGIAQG"
     gene            complement(313..594)
                     /locus_tag="WGQ68_001513"
     CDS             complement(313..594)
                     /locus_tag="WGQ68_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nosX protein required for nitrous oxide
                     reduction"
                     /protein_id="HEZ0715291.1"
                     /translation="MGRHFGRRRFLTVAAVAAAGAAVSFLPNPFAADDEKRNGDEKRN
                     ENVFFWKGVALGSGAELRLFGVDDRRAADLVNKVLAEVARLEKIVQPLP"
     gene            complement(603..1397)
                     /locus_tag="WGQ68_001514"
     CDS             complement(603..1397)
                     /locus_tag="WGQ68_001514"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEZ0715292.1"
                     /translation="MKAYLDLMRHVLDNGTDKSDRTGTGTRSVFGYQMRFDLGKGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLKDNNVSIWDEWADENGDLGPVYGYQWRNWP
                     APDGRHIDQIANVVEQIKKNPDSRRLIVSAWNPALVDEMALPPCHALFQFYVADGKLS
                     CQLYQRSADIFLGVPFNIASYALLTMMMAQVCGLEAGEFIHTFGDAHLYRNHFEQAAL
                     QLEREPRALPVMKINPEVKDLFSFKFEDFELEGYDPHPHIKAAVSV"
     gene            1820..3154
                     /gene="gdhA"
                     /locus_tag="WGQ68_001515"
     CDS             1820..3154
                     /gene="gdhA"
                     /locus_tag="WGQ68_001515"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEZ0715293.1"
                     /translation="MTDLNTLFANLKQRNPNQEPFHQAVEEVFMSLDPFLAKNPKYTQ
                     QSLLERIVEPERVVMFRVTWQDDKGQVQVNRGYRVQMSSAIGPYKGGLRFHPTVDLGV
                     LKFLAFEQVFKNALTTLPMGGGKGGSDFDPKGKSDAEVMRFCQAFMTELYRHIGADTD
                     VPAGDIGVGGREIGYLFGQYKKIRNEFSSVLTGKGLEWGGSLIRPEATGYGCVYFAQA
                     MLQTRNDSFEGKRVLISGSGNVAQYAAEKAIQLGAKVLTVSDSNGFVLFPDSGMTEAQ
                     LAALIELKEVRRERVATYAKEQGLQYFENQKPWGVAAEIALPCATQNELDEEAAKTLL
                     ANGCYVVAEGANMPSTLGAVEQFIKAGILYAPGKASNAGGVATSGLEMSQNAIRLSWT
                     REEVDQRLFGIMQSIHESCLKYGKVGDTVNYVNGANIAGFVKVADAMLAQGF"
     gene            3296..3670
                     /locus_tag="WGQ68_001516"
     CDS             3296..3670
                     /locus_tag="WGQ68_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715294.1"
                     /translation="MIMDQYYMELKNKLSNRPILLDNTNDFLFVLVNTVKAMIENTDK
                     SQLSELDKILDGVTSQELKLAYDFCQGKFGQAGFSYRRHPNYFYLSSLIATFPEFELS
                     KADRDYLKGIINFDNYLLYELD"
     gene            3714..3971
                     /locus_tag="WGQ68_001517"
     CDS             3714..3971
                     /locus_tag="WGQ68_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone acetyltransferase"
                     /protein_id="HEZ0715295.1"
                     /translation="MIELKLVDESSFQAVLDLKISEADERARFVAPNVRSLADAWLYR
                     ENEDVFPRAIYWDKQVVGFLLLEIDKDEAEYFIWRIMIGQQ"
     gene            complement(4157..4936)
                     /locus_tag="WGQ68_001518"
     CDS             complement(4157..4936)
                     /locus_tag="WGQ68_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEZ0715296.1"
                     /translation="MKLVRPQKISDQVLSVLEERIAEGVYAEGGKIPPERVLAEEFGV
                     SRPSVRSALNVLVARQVLEARQGDGYYVSVKPQQDFLQSWQELLGKHSNWEQDVFDFS
                     CHIEGCMAALAAERRTDADLKRIGFWLEKFEEACESGNLEHQSEADVSFHQTIADAAH
                     NLLFSHLSGGLLKMLYRQTRSSLIYLNQEEDPRPKLMAQHRVLYEAISNRRPGEASEA
                     AKAHLNYVASSILKDREYQSRNRHADTLAQNDLKRVQDWEV"
     gene            5367..5666
                     /locus_tag="WGQ68_001519"
     CDS             5367..5666
                     /locus_tag="WGQ68_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HEZ0715297.1"
                     /translation="MQRCFNWFDHYCRRLEKYFRRCSIMETWVQNYTAIGGSLYLTAA
                     AALLPIVFFFAALTVLKLKGYQAGLYTLLIALAVAVFGFGMPAGMAVSSLPPQPH"
     gene            5663..5842
                     /locus_tag="WGQ68_001520"
     CDS             5663..5842
                     /locus_tag="WGQ68_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tonB dependent receptor family protein"
                     /protein_id="HEZ0715298.1"
                     /translation="MTQRPYATLYLTAHYKIGKSTRIGLDFENVFNKRYRPMPDIHVY
                     GTPRSLTATVKHIVD"
     gene            complement(6034..7437)
                     /gene="mtrE"
                     /locus_tag="WGQ68_001521"
     CDS             complement(6034..7437)
                     /gene="mtrE"
                     /locus_tag="WGQ68_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MtrE"
                     /protein_id="HEZ0715299.1"
                     /translation="MDTTLKTTLTSVAAAFALSACTMIPQYEQPKVEVAETFKNDTAD
                     SGIRAVDLGWHDYFADPRLQKLIDIALERNTSLRTAVLNSEIYRKQYMIERNNLLPTL
                     AANANDSRQGSLSGGNVSSSYKVGLGAASYELDLFGRVRSSSEAALQGYFASTANRDA
                     AHLSLIATVAKAYFNERYAEEAMSLAQRVLKTREETYKLSELRYKAGVISAVALRQQE
                     ALIESAKADYAHAARSREQARNALATLINQPIPDDLPAGLPLDKQFFVEKLPAGLSSE
                     VLLDRPDIRAAEHALKQANANIGAARAAFFPSIRLTGSVDTHSAELGGLFKSGTGVWS
                     FAPSITLPIFTWGTNKANLDAAKLRQQAQIVAYEAAVQSAFQDVANALAAREQLDKAY
                     DALSKQSRASKEALRLVGLRYKHGVSGALDLLDAERSSYSAEGAALSAQLTRAENLAD
                     LYKALGGGLKRDTQTDK"
     gene            complement(7492..10695)
                     /gene="mtrD"
                     /locus_tag="WGQ68_001522"
     CDS             complement(7492..10695)
                     /gene="mtrD"
                     /locus_tag="WGQ68_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MtrD"
                     /protein_id="HEZ0715300.1"
                     /translation="MAKFFIDRPIFAWVISIFIIAAGIFGIKSLPVSQYPSVAAPTIT
                     LRATYPGASAQVMEDSVLSVIERNMNGVEGLDYMSTSADSSGSGSVSLTFTPDTDENL
                     AQVEVQNKLSEVLSTLPATVQQYGVTVSKARSNFLMIVMLSSDVQSTEEMNDYAQRNI
                     VPELQRIEGVGQVRLFGAQRAMRIWVDPKKLQNYNLSFADVGSALSAQNVQISAGSIG
                     SLPAVRGQTVTATVTAQGQLGTAEEFGNVILRANTDGSNVYLKDVARVGLGMEDYSSS
                     TRLNGVNTTGMAVMLSNSGNAMATAKAVKERMATLEKYFPQGMSWKTPYDTSKFVEIS
                     IEKVIHTLIEAMVLVFVVMYLFLQNIRYTLIPTIVVPISLLGGFAFISYMGMSINVLT
                     MFAMVLVIGIVVDDAIVVVENVERIMAGEGLPPKEATKKAMGQISGAVIGITAVLISV
                     FVPLAMFSGATGNIYKQFALTMASSIAFSAFLALTLTPALCATMLKTIPKGHHEEKKG
                     FFGWFNKKFNSWTHGYEGRVAKVLRKTFRMMVVYIGLAVVGVFLFMRLPTSFLPTEDQ
                     GFVMVSVQLPAGATQERTNATLAQVTQLAKSIPEIENIITVSGFSFSGSGQNMAMGFA
                     ILKDWNERTAPGSDAVAIAGKLTGMMMGTLKDGFGIAVVPPPILELGNGSGLSINLQD
                     RNNTGHTALLAKRNELIQKMRASGLFDPSTVRAGGLEDSPQLKIDINRAAAAAQGISF
                     ADIRTALASALSSSYVSDFPNQGRLQRVMVQADEDARMQPADILNLTVPNKSGVAVPL
                     STIATVSWENGTEQSVRFNGYPSMKLSASPATGVSTGQAMAAVQKMVDELGGGYSLEW
                     GGQSREEAKGGSQTLILYGLAVAAVFLVLAALYESWSIPLAVILVIPLGLIGAAAGVT
                     GRNLFEGLLGSVPSFANDIYFQVGFVTVMGLSAKNAILIIEFAKDLQAQGKSAVEAAL
                     EAARLRFRPIIMTSFAFILGVVPLYIAGGASSASQRAIGTTVFWGMLIGTLLSVFLVP
                     LFYVVVRKFFKETAHEHEMAVKHAAEAGITGSDDSQH"
     gene            complement(10707..11945)
                     /gene="mtrC"
                     /locus_tag="WGQ68_001523"
     CDS             complement(10707..11945)
                     /gene="mtrC"
                     /locus_tag="WGQ68_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit MtrC"
                     /protein_id="HEZ0715301.1"
                     /translation="MAFYAFKAMRAAALAAAVALVLSSCGKGGDAAQGGQPAGREAPA
                     PVVGVVTVHPQTVALTVELPGRLESLRTADVRAQVGGIIQKRLFQEGSYVRAGQPLYQ
                     IDSSTYEAGLESARAQLATAQATLAKADADLARYKPLVAAEAVSRQEYDAAVTAKRSA
                     EAGVKAAQAAIKSAGISLNRSRITAPISGFIGQSKVSEGTLLNAGDATVLATIRQTNP
                     MYVNVTQSASEVMKLRRQIAEGKLLAADGVIAVGIKFDDGTVYPEKGRLLFADPAVNE
                     STGQITLRAAVPNDQNILMPGLYVRVLMDQVAVDNAFVVPQQAVTRGAKDTVMIVNAQ
                     GGMEPREVTVAQQQGTNWIVTSGLKDGDKVVVEGISIAGITGAKKVTPKEWASSENQA
                     AAPQSGVQTASEAKPASEAK"
BASE COUNT         2960 a         3327 c         2990 g         2740 t
ORIGIN      
        1 ccttgcgcga tgccgttcaa ggacaaaccc ataccctttt cggcaaaacg gatttcacgg
       61 tcgtcaaacg agactttgcc gaagccgacc cgtttcaggg cttcgtccac gctgttttga
      121 ggaggcggcg tttccgcatc gggacgggcg gcaaaataat cggcatacag tttccacaac
      181 gcctgcactg tcggatcgaa cgcgccgtcc gtcagcgcgt gaatatcgcg gcacaggctc
      241 aacagttcca aaaaatccgc cgacggcgaa gtcagataac cgtccctgtt caggcggctg
      301 atcaggctgt cttcacggta aaggctgaac aattttttcc aaacgcgcca cttccgccaa
      361 aaccttgttg accaaatccg ccgcacgcct gtcgtccaca ccgaacagac ggagctccgc
      421 accggaaccc agtgcgacac ctttccagaa aaacacattt tcattgcgtt tttcatcccc
      481 gttgcgtttt tcatcatcgg cggcaaaagg attcggcagg aaagaaaccg ccgcgcccgc
      541 cgccgcaacg gcggcaaccg tcagaaaacg cctgcgcccg aaatgcctgc ccataccgcc
      601 tcctaaaccg acactgccgc cttgatatgc ggatgagggt cgtaaccttc caactcgaaa
      661 tcttcaaact tgaaggaaaa caaatctttg acttcaggat tgattttcat cactggcaag
      721 gcgcgcggtt cgcgttccaa ctgcaatgcg gcctgctcga aatggttgcg gtacaaatgc
      781 gcgtcgccaa acgtatggat aaactcaccg gcttccaatc cgcacacttg cgccatcatc
      841 atggtcaaca atgcgtagct ggcgatgttg aacggtacgc caaggaaaat atctgcacta
      901 cgctggtaaa gctggcagga cagtttgccg tcggcaacgt aaaactgaaa cagcgcgtgg
      961 caaggcggca aggccatctc atcgaccaaa gccggattcc acgccgatac aatcaggcgg
     1021 cgcgagtcgg ggtttttctt aatttgttcc accacattgg cgatttggtc gatatggcgg
     1081 ccgtcgggcg cgggccagtt gcgccactgg tagccgtaaa ccgggcccaa gtcgccgttt
     1141 tcgtccgccc actcgtccca aatggaaaca ttgttgtcct tcaggtattt gatattggta
     1201 tcgcctttga gaaaccaaag cagctcgtgg ataatcgaac gcagatgcag ctttttggtc
     1261 gtcagcagcg gaaaaccttt gcccaagtca aaacgcatct gataaccgaa tacggagcgc
     1321 gtgcccgtac cggtgcggtc tgatttgtcc gtaccgttgt cgaggacgtg gcgcatcaag
     1381 tccaaatagg ctttcatagc agtctttcat caaattaaac ggcgcatatt gtaacatttc
     1441 cggccaacgc ccgagtccgg acaaacgcac acgccgcccc gccggacgat aaaaacgcag
     1501 atacgggcag gcaggattgt tggcaatttc agtccttttc cacagtaaaa cccggtggga
     1561 aaacaaaatt accttgattg gaatcaaaaa atctagttta attacttaga ataaaatttc
     1621 aataatattt ttatttacga aattaattta tgattattga ttaattatcg gcaacaaaca
     1681 tcaaacatcg aaaacatgga aaaaataatg tcaacaattt ttgccaaatc gggcttggca
     1741 tcagaaaaaa ataggtttat attcccacct acaaatttgt tttcccatta gtacactatc
     1801 aaccaaaagg agtatccgaa tgactgacct gaacaccctt tttgccaacc tcaaacaacg
     1861 caatcccaat caggagccgt tccatcaggc ggttgaagaa gtcttcatga gtctcgatcc
     1921 gtttttggca aaaaatccga aatacaccca gcaaagcctg ctggaacgca tcgtcgaacc
     1981 cgaacgcgtc gtcatgttcc gcgtaacctg gcaggacgat aaagggcaag tccaagtcaa
     2041 ccggggctac cgcgtgcaaa tgagttccgc catcggtcct tacaaaggcg gcctgcgctt
     2101 ccatccgacc gtcgatttgg gcgtattgaa attcctcgct tttgaacaag tgttcaaaaa
     2161 cgccttgacc accctgccta tgggcggcgg caaaggcggt tccgacttcg accccaaagg
     2221 caaatccgat gccgaagtaa tgcgcttctg ccaagccttt atgaccgaac tctaccgcca
     2281 catcggcgcg gacaccgatg ttccggccgg cgacatcggc gtaggcgggc gcgaaatcgg
     2341 ctacctgttc ggacaataca aaaaaatccg caacgagttt tcttccgtcc tgaccggcaa
     2401 aggtttggaa tggggcggca gcctcatccg tcccgaagcg accggctacg gctgcgtcta
     2461 tttcgcccaa gcgatgctgc aaacccgcaa cgatagtttt gaaggcaaac gcgtcctgat
     2521 ttccggttcc ggcaacgtgg cgcaatacgc cgccgaaaaa gccatccaac tgggcgcgaa
     2581 agtgctgacc gtttccgact ccaacggctt cgtcctcttc cccgacagcg gtatgaccga
     2641 agcgcaactc gccgccttga tcgaattgaa agaagtccgc cgcgaacgcg ttgccaccta
     2701 cgccaaagag caaggtctgc aatactttga aaaccaaaaa ccgtggggcg tcgccgccga
     2761 aatcgccctg ccctgcgcga cccagaacga attggacgaa gaagccgcca aaaccctgtt
     2821 ggcaaacggc tgctacgtcg ttgccgaagg tgcgaatatg ccgtcgactt tgggcgcggt
     2881 cgagcaattt atcaaagccg gcatcctcta cgccccggga aaagcctcca atgccggcgg
     2941 cgtagcaact tcaggtttgg aaatgagcca aaacgccatc cgcctgtctt ggactcgtga
     3001 agaagtcgac caacgcctgt tcggcatcat gcaaagcatc cacgaatcct gtctgaaata
     3061 cggcaaagtc ggcgacacag taaactacgt caatggtgcg aacattgccg gtttcgtcaa
     3121 agttgccgat gcgatgctgg cgcaaggctt ctaagcaaac gccgccgctc cgcaaacaaa
     3181 atgccgtccg aaccgcaaat gctgttcaga cggcatttca ttatccgccc gttcgaatcg
     3241 ggtgatacta tttgtatcca gaattgttgg atagtttggg agtggagagg aagaaatgat
     3301 tatggaccaa tactatatgg agttaaagaa taagttatct aacagaccaa tcctactgga
     3361 taatactaat gattttcttt ttgttttagt aaatacagtg aaagctatga tagaaaatac
     3421 tgataaaagt cagctttcag agttagataa aattttagac ggtgtcacaa gtcaagaatt
     3481 gaaacttgcc tatgattttt gtcaagggaa attcggtcaa gcaggttttt cttatcgtag
     3541 gcacccgaat tatttttatt tgtccagttt aattgcgact tttccagaat ttgagttgtc
     3601 taaagcagac cgagattacc tcaaaggaat cataaatttt gataactacc ttttatatga
     3661 gttagattag tctttaatca cctcaaaacc ctatttattc aaaaggagaa aaaatgattg
     3721 agctgaaatt agtagatgag agcagttttc aggcagtgct ggatttgaaa atatctgaag
     3781 ctgatgaacg agcacgtttt gtggctccaa atgtacgctc tttagctgac gcatggctct
     3841 accgggaaaa tgaagatgtg tttccgaggg caatctattg ggataagcaa gtggttggct
     3901 ttctcctgct ggaaatagac aaggatgaag cagaatactt tatctggcgg ataatgattg
     3961 gtcagcagtg aattaacaaa aatcaggaca aggcggcgag ccgcagacag tacaaatagt
     4021 acggaaccga ttcacttggt gcttcagcac cttagagaat cgttctcttt gagctaaggc
     4081 gaggcaacga cgtactggtt taaatttagt tcactatacc gatacatccg aatatgccag
     4141 accgtctaaa cggtattcat acctcccaat cctgcacgcg cttcaaatca ttttgcgcca
     4201 aagtatctgc gtggcggtta cggctctgat attccctgtc tttcaagatg ctgctcgcca
     4261 cataattcaa atgtgccttt gccgcctccg aagcctcgcc cggccggcgg tttgatattg
     4321 cctcatacaa tacacggtgc tgcgccatca gcttcggacg cggatcttct tcctgattca
     4381 gataaataag gctgctgcgc gtctgccggt acagcatttt caacaaaccg cccgacaaat
     4441 ggctgaacaa caaattgtgc gccgcatccg caatcgtctg atggaagctg acatcggctt
     4501 cgctctgatg ttccagattg ccgctttcgc acgcctcctc aaacttttca agccaaaacc
     4561 caatccgctt caaatcggca tccgtccggc gttctgccgc caatgccgcc atacagccct
     4621 cgatgtggca gctgaaatca aaaacatcct gttcccaatt ggaatgcttg cccaaaagct
     4681 cctgccaact ttgcaaaaaa tcctgctgcg gcttgaccga aacataataa ccgtctccct
     4741 gcctcgcttc caaaacctga cgggcaacca aaacattcaa tgccgaccgg accgacgggc
     4801 gcgaaacgcc gaactcttcc gccaaaacgc gttcgggcgg aatcttgccc ccttccgcgt
     4861 aaaccccttc cgcaatgcgc tcctccaata ccgacaatac ctgatcgctg attttctgag
     4921 gccttaccag tttcatcact cctcctttat aaagattccc tgccgaaccc ttccgaaata
     4981 tagtggatta acaaaaatca ggacaaggtg acgaagccgc agacagtaca aatagtacgg
     5041 aaccgattca ctcggtgctt cagcacctta gagaatcgtt ctctttgagc taaggcgagg
     5101 caacgccgta ctggtttttg ttcatccact atacatcaaa catcaaattg gactgaccaa
     5161 tcagggcgga ttctaatgac acgccgttcc cgccgtcaac ggcatttacc tcgcaccgcc
     5221 cccgaaacac aagaaaaaac tacaccaaac tacaattttt gttcatgcaa atatttgttt
     5281 tgacaggatt taaacgaaag ctccgattca aatctgccga accgcccaaa aatatattga
     5341 cctaaatatt aaagtttcgc aaagtaatgc aacgttgctt taattggttt gaccactatt
     5401 gccgacgatt agaaaaatat tttcggagat gttcaattat ggaaacttgg gttcaaaact
     5461 acacggcaat cggcggcagc ctgtatctga ctgccgccgc cgcactctta cccatcgtct
     5521 ttttctttgc cgcgttgacc gtcctgaagc tgaaaggcta tcaggcgggg ctttatacgc
     5581 tgctgattgc gcttgccgtt gccgtattcg gcttcgggat gccggcgggt atggcggttt
     5641 cttccctgcc gccgcagccg cattgaccca acgcccctac gccacactgt atttgaccgc
     5701 gcattacaaa atcggcaaat ccacccgcat cggtttggac tttgaaaacg tgttcaacaa
     5761 acgctaccgc cctatgcccg acattcacgt ttacggcacg ccgcgcagcc tgaccgcaac
     5821 cgtcaaacat atagtggatt aacaaaaatc aggacaaggc gacgaagccg cagacagtac
     5881 aaatagtaca acttggtgct tcagcacctt agagaatcgt tctctttaag ctaaggcgag
     5941 ccaacgccgt actggttttt gttaatccac tataaagaaa gaaatgccgt ctgaaacctt
     6001 atcgtttcag acggcatttt attgcccgac cggttatttg tcggtttggg tatcccgttt
     6061 caatccgccg ccgagtgcct tgtacaaatc ggcaaggttt tcggcgcggg tcagttgtgc
     6121 cgacaaagcc gcaccttccg ccgaatagct gctgcgttcc gcatcgagca aatcaagcgc
     6181 gccggatacg ccgtgtttgt aacgcagacc gaccaaacgc aacgcttctt tagaggcgcg
     6241 gctttgtttg cttaaagcgt cataggcttt atccagctgc tcgcgcgccg ccaatgcgtt
     6301 tgccacgtct tgaaaggcgg attggacggc ggcttcatag gcaacgattt gtgcctgttg
     6361 gcgcagcttg gctgcatcaa ggttggcttt gttcgtgccc caggtaaaaa tcggcagggt
     6421 aatagacggc gcaaacgacc aaacgcccgt gccgcttttg aacagcccgc ccaattcggc
     6481 agaatgcgta tcgacgcttc cggtcaggcg gatggatggg aaaaaggcgg cgcgtgccgc
     6541 accgatattg gcgtttgcct gtttgagcgc gtgttcggca gcacggatat cgggacggtc
     6601 gagcaatact tcggaactca aaccggccgg cagcttctca acaaaaaact gcttgtccaa
     6661 cggcaaaccg gcgggcaggt cgtcgggtat cggttggtta atcagggttg ccaaggcatt
     6721 gcgcgcctgt tcgcggctgc gcgcggcatg ggcataatcg gctttggcag attcgattag
     6781 ggcttcctgc tgacgtaggg cgacggcgga aatcacgcct gccttgtaac gtaattcgga
     6841 cagcttgtag gtttcctcgc gcgttttcaa aacacgttgc gccaaagaca tcgcttcttc
     6901 ggcgtaacgt tcgttgaaat aggctttggc aacggtggca atcaggctca aatgtgccgc
     6961 atcgcggttg gcggtgctgg cgaaatagcc ttgcagtgcc gcctcgctgc tgctgcgtac
     7021 acgcccgaac agatcgagtt cgtaagatgc cgcacccagt ccgactttgt agctgctgct
     7081 tacattgccg ccgctcaagc tgccttggcg cgagtcgttc gcattggcgg caagcgtggg
     7141 caggaggttg ttgcgctcaa tcatgtattg tttgcggtag atttcgctgt tcaatacggc
     7201 ggtacgcaaa ctggtattgc gctcgagtgc gatgtcgatc agcttttgca ggcgcgggtc
     7261 ggcaaaatag tcatgccaac ctaaatcgac ggcgcggatg ccgctgtcgg cggtatcgtt
     7321 tttgaacgtt tcggcaactt cgactttggg ctgctcgtat tggggaatca tggtgcaggc
     7381 agacaatgca aaggctgctg caacagaagt caaggtggtt ttcaatgtag tatccataaa
     7441 aaagtcctga tgccgtctga aaacccgtgg gcgttcagac ggcatggttg cttaatgttg
     7501 gctgtcgtcc gaaccggtga tgcccgcttc ggcggcgtgt tttactgcca tttcgtgttc
     7561 gtgcgcggtt tctttgaaga atttgcgcac caccacatag aaaagcggaa caaggaacac
     7621 ggacaagagc gtgccgatga gcatccccca gaatacggtt gtaccgatgg cgcgctggct
     7681 ggcagaactt gcaccgccgg caatatacag gggaaccacg cccaaaataa aggcgaacga
     7741 ggtcatgata atcggacgga aacgcaggcg ggcggcttcc aaagcggctt caaccgcgct
     7801 tttcccttgc gcttgaaggt ctttggcaaa ttcgataatc aaaatcgcat ttttcgcact
     7861 caaacccatc acggtaacga aaccgacttg aaagtagatg tcgttggcga acgagggaac
     7921 gctgcccaac agtccttcaa acaggttgcg cccggttacg cccgcagccg caccgatcaa
     7981 acccaacgga atcacaagga tgaccgccag cggaatcgac cagctttcat aaagcgcggc
     8041 aagtaccaaa aatacggctg caaccgccaa accgtacaaa atcagggttt gcgagccgcc
     8101 ttttgcctct tcgcgcgact gtccgcccca ctccaggctg taaccgccgc ccaattcgtc
     8161 aaccattttt tgaaccgccg ccatagcctg cccggtggaa acgccggttg caggcgaagc
     8221 ggacagcttc atcgaaggat aaccgttgaa gcgtacgctc tgttccgtac cgttttccca
     8281 agaaacagta gcaatggtgg aaagcggtac ggcgacgccg gatttgttcg gcacggtcag
     8341 gttcaaaata tcggcaggct gcatacgggc atcctcgtcg gcctgcacca tcacgcgttg
     8401 cagacggcct tggttcggga agtcgctgac ataagacgaa ctcagcgcgc ttgccaatgc
     8461 ggtgcggatg tcggcaaacg aaatgccttg cgccgccgcg gcggcacggt tgatgtcgat
     8521 tttcaactgc ggcgagtctt ccaaaccgcc ggcacggacg gtgctggggt caaacaaacc
     8581 gctggcacgc attttctgaa tcaactcgtt gcgcttcgcc agcaatgcgg tatggccggt
     8641 attattgcgg tcttgcaggt tgatgctcag gcccgaacca ttgcccaact ccagaatcgg
     8701 aggcgggacg acggcgatgc caaaaccgtc tttaagcgtc cccatcatca tacccgtcag
     8761 cttgccggca atcgcaacgg catcgctgcc gggcgcggta cgctcgttcc aatctttcaa
     8821 tatggcaaaa cccatcgcca tattctgacc gctgcccgaa aagctgaagc cggaaacggt
     8881 aatgatgttt tctatttcag gaatgctttt cgccagttgg gtaacttgcg ccaaagtcgc
     8941 attggtgcgc tcttgggtcg ctcctgcagg cagttgcacg ctgaccatga cgaagccttg
     9001 gtcttcggtc ggcaggaatg aagtcggcag acgcataaac aggaacacgc ccacaaccgc
     9061 caagccgata tagacaacca tcatgcggaa agtcttacgc agcactttgg caacccggcc
     9121 ttcgtaaccg tgcgtccaac tgttgaattt cttgttaaac cagccgaaga aacctttttt
     9181 ctcttcgtga tgccctttcg ggattgtctt caacattgtg gcacacaaag caggggtaag
     9241 ggtcagcgca aggaaggcgg agaatgcgat tgatgacgcc atcgtcaggg caaactgttt
     9301 gtaaatattg cccgtcgccc cgctgaacat cgccaacggt acgaacacgg aaatcagaac
     9361 ggcggtaata ccgatgaccg cgcccgaaat ctgacccatc gcttttttgg tcgcttcttt
     9421 gggcggcaag ccttcacccg ccataatgcg ctcgacgttt tcaaccacca caatcgcgtc
     9481 atcgaccacg atgccgatga ccaaaaccat cgcaaacatg gtcagtacgt taatcgacat
     9541 gcccatataa gagatgaaag cgaaaccgcc caacagcgaa atcggtacga caatggtcgg
     9601 aatcagcgta taacggatgt tttgcaggaa gagatacatt acgacaaaca ccagcaccat
     9661 cgcttcgatt aaagtgtgaa tcactttttc aatcgaaatt tcgacgaatt tggaagtatc
     9721 gtaaggggtt ttccagctca taccctgagg aaagtatttt tccaacgtcg ccatgcgttc
     9781 tttaaccgcc tttgccgtcg ccatcgcatt gccgctgttg gacagcatca ccgccatacc
     9841 ggtggtattt acaccgttca gacgggttga ggaagaatag tcttccatac ccagtccgac
     9901 ccttgccaca tccttcaggt aaacattaga accgtcggta ttggcgcgga ggatgacgtt
     9961 gccgaattct tctgccgtac ccaactgccc ttgcgccgtt acggtagccg taaccgtctg
    10021 tccgcgaacg gcgggaagcg aaccgataga acccgctgaa atctggacgt tctgggcgga
    10081 cagcgcgctg ccaacatcgg caaacgacaa attgtagttt tgcagtttct taggatcaac
    10141 ccaaatccgc atcgcgcgtt gcgcgccgaa caggcgtacc tgccccacgc cttcgatacg
    10201 ctgcaactcg ggaacgatat tacgctgcgc gtagtcgttc atctcttcgg ttgactgcac
    10261 atccgacgaa agcatcacaa tcatcaggaa attggaacgc gccttggata cggttacgcc
    10321 gtattgctgg acagttgccg gcagcgtgct caatacttcg gaaagcttgt tctgcacttc
    10381 cacctgcgcc agattctcgt cggtatcggg cgtaaaggtc aggctcacgc tgccgctgcc
    10441 gctcgaatcg gcggaagtgg acatataatc caaaccttcc acgccgttca tattccgctc
    10501 gatcacggaa agcacgctgt cttccattac ctgcgcggac gcgcccggat aagtggccct
    10561 cagggtgatg gtcggggcgg cgacggacgg atattgcgaa accggcaggc ttttgatgcc
    10621 gaaaataccc gccgcaataa tgaaaatcga aataacccac gcaaaaatgg ggcggtcgat
    10681 aaaaaattta gccatcgatg ccttccttat ttcgcttcag aagcaggttt ggcttcagat
    10741 gccgtctgaa cgccggattg aggcgcggcg gcttggtttt cagacgacgc ccattctttg
    10801 ggcgttacct ttttcgcacc cgttataccg gcgatactga tgccttccac aaccaccttg
    10861 tccccgtcct tcagacccga cgtaacaatc caattcgtac cctgctgttg cgcaaccgtt
    10921 acctcgcggg gttccatacc gccttgggca ttcacaatca tcacggtatc tttcgcaccg
    10981 cgcgttaccg cctgctgcgg cacaacaaat gcgttatcca ccgccacttg gtccatcagc
    11041 acgcgcacat acagaccggg catcaagata ttctgatcgt tcggtacggc ggcgcgcagg
    11101 gtaatctgac cggtcgattc gttgacggcc ggatcggcaa acagcaggcg gcctttttca
    11161 gggtaaactg tgccgtcgtc aaatttgatg ccgaccgcaa tcacaccatc cgccgccagc
    11221 agtttgcctt cggctatctg acggcgcaat ttcatcactt cggatgcaga ctgggtaacg
    11281 ttcacataca tcggattggt ttggcggatg gtcgccagta cggtcgcatc gccagcgttc
    11341 agcaacgtac cttcggaaac tttggactga ccgataaagc cggaaatcgg cgcggtaatg
    11401 cgcgaacggt tcaggctgat gccggcggat ttgattgccg cctgcgccgc tttaacgcct
    11461 gcctcggcag aacgtttcgc cgttaccgca gcatcgtatt cctgccggct gacggcttcg
    11521 gcggcaacca aaggcttgta tcgcgccaaa tccgcatccg ctttggcaag cgttgcctga
    11581 gccgttgcca gttgcgcgcg cgcgctttcc agacctgctt cataagtgga actgtcgatc
    11641 tgatacagcg gctgtccggc acggacataa ctgccttctt ggaacaggcg tttttggatg
    11701 atgccgccga cttgggcgcg gacatcggcg gtacgcagcg attccaaacg ccccggcaac
    11761 tcgacggtca atgcgacggt ttgcggatgg acggttacga caccgacgac gggcgcaggg
    11821 gcttcccgac cagcaggctg cccgccctgc gccgcgtctc cgcctttacc gcaagacgac
    11881 agtaccaatg caacggcggc agccaacgcg gccgcacgca tcgccttaaa agcataaaaa
    11941 gccattattt atcctatctg tctggtttga tgtaaagggt tttgccaatc aacaggcatt
    12001 cttatagtgg attaaca
//
LOCUS       DAWFHY010000065        11935 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.065, whole genome shotgun sequence.
ACCESSION   DAWFHY010000065 DAWFHY010000000
VERSION     DAWFHY010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11935
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.065"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..81
                     /locus_tag="WGQ68_001524"
     CDS             <1..81
                     /locus_tag="WGQ68_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0715302.1"
                     /translation="GWPLQTAPLLSPKDLAGKVLAEAVTF"
     gene            105..2219
                     /locus_tag="WGQ68_001525"
     CDS             105..2219
                     /locus_tag="WGQ68_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein"
                     /protein_id="HEZ0715303.1"
                     /translation="MFLFSDGLQSINNNNRRKRIVKNAYIPSRGIRKIPHLSTLLPEF
                     HIYKDGKGAEAVVGWGLRPTTHKARAFATEHQLPFIALEDGFLRSLGLGVSGYPPYSI
                     VYDDIGIYYDTTRPSRLEQLILAADTMPSETLAQAQQTMDFILQHHLSKYNHAPELSD
                     DHPLRSPSKPETVLIIDQTFGDMAIQYGGADASTFELMFQTALNENPQADIWVKTHPD
                     VLCGKKQGYLTQLAQQHRVHLLAEDINPISLLQNVDKVYCVTSQMGFEALLCGKPLTT
                     FGLPWYAGWGVSDDRHPKIGSLIQTQRRAPRNLLQLFAAAYLQYSRYLNPNTGEAGSL
                     FDVIDYLATVKRKNDKLRGELYCVGMSLWKRAVAKPFFNVPSCRLKFISSTQKLARVK
                     LSDDARILAWGNGKEAIVRFAEQHHIPLLRMEDGFIRSVGLGSNLVPPLSLVTDDMSI
                     YFNAETPSRLEYILQNQNFDDQDFQTALKLQKMLTENHISKYNVGSSDFTAPSTDKTV
                     ILVPGQVEDDASIRYGSPQIYRNLDLLRTVRERNPNAYIIYKPHPDVVSGNRIGHISP
                     EDAARYADQTAEQADILTCLQYADEIHTMTSLTGFEALLRGKKVSCYGLPFYAGWGLT
                     QDLLPIPRRSRRLELWQLIAGTLIHYPDYIHPETHQAINAETAAQILIRQKNMQKNNN
                     GLHRGCFAKKLGKIKQLYRSFK"
     gene            2356..3615
                     /locus_tag="WGQ68_001526"
     CDS             2356..3615
                     /locus_tag="WGQ68_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis protein"
                     /protein_id="HEZ0715304.1"
                     /translation="MKQTVLKNNLQNLLESAENILLLQGPVGDFFLRLADWLTANGKT
                     VHKFNFNAGDDYFYPPTQAHTVVFNDNYDAFPEFLQEYITQHHIQAVVCFGDTRPYHV
                     IAKRIANENQASFWAFEEGYFRPYYITLEKDGVNAFSPLPRRADFFLEQFPKLAQQEY
                     KAPTPVHGGFTPMAKNAIRYYIELFRNPRKYPDYIHHRAPNAGHYLKPWSLSILKRLN
                     YYIEDIQIAKRVEAGKYGKFFIVPLQVFNDSQVRIHCDFPSVRSFLLHVLSSFAEHAP
                     ADTNIIIKHHPMDRGFIDYWRDIKRFIKEHPELQGRVIYVHDVPLPVFLRHGLGMVTI
                     NSTSGLSGLIHNMPVKVLGRAYYDIPGITDQNTLAEFWNHPTPPDKELFHAYRMYHLN
                     VTQINGNFYSQVFFPNKKTSDSSTPAT"
     gene            3982..5388
                     /locus_tag="WGQ68_001527"
     CDS             3982..5388
                     /locus_tag="WGQ68_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HEZ0715305.1"
                     /translation="MTAAPDILYRQAAALLEQSNTAQALPLLQQAAEQGYTEAAFVLG
                     NHLLKNGHPEQALPWLEAAAAQRHPKALFSLLQQREHNGTPTGQLLNDYAWLGEQGHS
                     EAQLILMRYHAQRNDPQSLYWAELAAARYAAPAYYHLARHHQRQGDVETAIEQYEKAA
                     ALGVTAACWQLGQIYFYGTGVSPNHAQAEHYLEPAAQAGHIAAQTLLADLLAAQRKPE
                     ALEWYRRAADKEQAEAQSKLAQYALTGELSERDPFQAARYAKAAAEKNHPEALKIMGD
                     LYRYGLGIKADNHIAHDYYHRAAALGSAAAAQKLISDAALYHPQQYEQIKTAALQQQQ
                     TETIYRLAETQAHAIGRPADYNAARKNYMEAAEFHHKNAAAALGRIYHYGLGTAQDPR
                     AAAHWYAIAAEQNHPSAQYHLACFYYHGQGVGCHVPTACYWLQAAISNGHTSAESLIS
                     LLEQWRREAHHAIGQKAV"
     gene            5606..6820
                     /gene="gltS"
                     /locus_tag="WGQ68_001528"
     CDS             5606..6820
                     /gene="gltS"
                     /locus_tag="WGQ68_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HEZ0715306.1"
                     /translation="MEWEFNSYYTLIAATLVLLVGKFLVQKIKFLRDFNIPEPVAGGL
                     VAAIILFALHEAYGVSFKFEKPLQNAFMLIFFTSIGLSADFSRLKAGGLPLVVFTAIV
                     GGFILVQNFVGVGLATALGLDPLIGLITGSVSLTGGHGTSGAWGPNFETQYGLVGATG
                     LGIASATFGLVFGGLIGGPVARRLINKMGRKPVENKKQDQDDNADDVFEQAKRTRLIT
                     AESAVETLAMFAACLAFAEIMDGFDKEYLFDLPKFVWCLFGGVVIRNILTAAFKVNMF
                     DRAIDVFGNASLSLFLAMALLNLKLWELTGLAGPVTVILAVQTVVMVLYATFVTYVFM
                     GRDYDAAVLAAGHCGFGLGATPTAVANMQSVTHTFGASHKAFLIVPMVGAFFVDLINA
                     AILTGFVNFFKG"
     gene            6919..7935
                     /locus_tag="WGQ68_001529"
     CDS             6919..7935
                     /locus_tag="WGQ68_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1311 domain-containing protein"
                     /protein_id="HEZ0715307.1"
                     /translation="MYRKLIALPFALLLAACGREEPPKALECANPAVLQGIRGNIQET
                     LTQEARSFAREDGRQFVDADKIIAAAYGLAFSLEHASETQEGGRTFCIADLNITVPSE
                     TLADAEANSSLLYGETSFADIVRQKTGGNVAFKDGVLTAAVRFLPVKDGQTAFVDNTV
                     GMAAQTLSAALLPYGVKSIVMIDGKAVKKEDAVRILSGKAREEEPSKPTPEDILEHNA
                     AGGDADVPQAAEGAPEPEILHPDDGERADTVTVSRGEVEEARVQNQRAESEITKLWGG
                     LDTDVQKELVGEQRKWAQEKISNCRQAAAQADRQEYAEYLKLQCDTRMTRERIQYLRG
                     YSID"
     gene            7946..8143
                     /locus_tag="WGQ68_001530"
     CDS             7946..8143
                     /locus_tag="WGQ68_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715308.1"
                     /translation="MTTVPKSRIPVKPLPEKTTDEAKVEKWRQLGAEHGLSGEWAVAA
                     RLGENGFTEEQMENIANLFGR"
     gene            8161..8310
                     /locus_tag="WGQ68_001531"
     CDS             8161..8310
                     /locus_tag="WGQ68_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715309.1"
                     /translation="MPSETLLSVSDGILTNTVRRRKTAGFSCVTYADVSTHRTIQKTS
                     PYVPS"
     gene            complement(8551..9945)
                     /locus_tag="WGQ68_001532"
     CDS             complement(8551..9945)
                     /locus_tag="WGQ68_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEZ0715310.1"
                     /translation="MTPSALKKTVLLLGTAFAAASVHASGYHFGTQSVNAQSTANAAA
                     AEAADASTIFYNPAGLTKLDSSQISVNANIVLPSIHYEADSATDFTGLPVQGSKSGKI
                     TKTTVAPHIYGAYKVNDNLTVGLGVYVPFGSATEYEKDSVLRHNINKLGLTSIAVEPV
                     AAWKLNDRHSFGAGIIAQHTSAELRKYADWGIMEKAKALKETPPNPTKAAQIKADGHA
                     DVKGSDWGFGYQLAWMWDINDRARVGVNYRSKVSHTLKGDAEWAADDAAAKAMWSTML
                     AANGYTANEKARVKIVTPESLSVHGMYKVSDKADLFGDVTWTRHSRFDKAELVFEKEK
                     TIGMGKKSDRTTITPNWRNTYKVGLGGSYQISEPLQLRAGIAFDKSPVRNADYRMNSL
                     PDGNRIWFSAGMKYHIGKNHVVDAAYTHIHINDTTYRTAKASGNDVDSKGASSARFKN
                     HADIIGLQYTYKFK"
     gene            complement(10255..11727)
                     /gene="pyk"
                     /locus_tag="WGQ68_001533"
     CDS             complement(10255..11727)
                     /gene="pyk"
                     /locus_tag="WGQ68_001533"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEZ0715311.1"
                     /translation="MNQTSRDLTRISHNTKIVATLGPGSNNVELLEDMIRVGGLNVVR
                     FNFSHGTPEFHQENARIVREAAKRAGQEIAIIADLQGPKIRVGKIAGGGIELNKGETL
                     VLDAALEGEGTREAVGLDYRDLPDDVAAGDVLWLDDGLLTLTVESVEGSRIITRVENS
                     HVLKSNKGINKRGGGLSAGALTEKDFRDLKTAIAIGCDYLAISFVKSAEDLHIARAKV
                     EEEMKGSTAVRPGLVSKIERVEAIENLDEIILAGDGIMVARGDLAVEVGHAAVPALQK
                     RMIRRARELRRFSITATQMMESMITNPVPTRAEVSDVANAVLDGTDAVMCSAETAVGA
                     YPFETVSQMAIICAAAEKEQDSLNGVAEQAEYPEAVSTNLAVAGGAVSVARAVHAKAI
                     VALTESGSTAFEISRHNITLPIFALTPSVSAQRRMAMYRGVRPLILATSTDHDTALNE
                     VETMLVEHNILHSGDQYIITSGSQMRESGSTNTLEVLRVK"
BASE COUNT         2795 a         3353 c         3002 g         2785 t
ORIGIN      
        1 ggctggccgc ttcaaaccgc gccgctgctg tcgcctaaag acttggccgg caaagtattg
       61 gctgaagcgg tcacgtttta agactattca ggccgtctga aagaatgttc ctcttttcag
      121 acggcctaca atctattaat aacaataatc gacgaaaacg cattgtgaaa aacgcctaca
      181 tcccctctcg cggcatccgc aaaatccccc atctctccac cctattgccc gagtttcata
      241 tctacaaaga cggaaaagga gcagaggctg ttgtcggctg gggtttgcgc ccgacgacac
      301 acaaagcgcg tgcttttgcc accgaacacc agcttccctt tattgctttg gaagacggct
      361 ttttacgctc gctcggactg ggcgtctccg gttatccccc ctactccatc gtctatgacg
      421 acatcggcat ctactacgac actacccgcc cttctcgttt ggaacaactg attcttgccg
      481 ccgataccat gccgtctgaa accttggctc aggcgcaaca gacgatggat ttcatcctgc
      541 aacaccacct gtccaaatac aaccacgcgc ccgaactttc agacgatcat cctttacgct
      601 ccccatccaa gcctgaaact gtcctcatta tcgaccaaac cttcggcgat atggccatcc
      661 aatatggcgg cgcagacgcc tctacgtttg aactgatgtt tcagacggcc ttgaatgaaa
      721 acccgcaagc cgatatctgg gtaaaaaccc atcccgatgt tttgtgcggc aaaaaacaag
      781 gctatctgac ccaactggcg cagcaacacc gcgtccatct tctggcagaa gacatcaacc
      841 cgatttcttt gttgcaaaac gtcgataaag tttattgcgt tacctcgcaa atgggttttg
      901 aggcactttt gtgcggcaag ccgctgacca ccttcggcct gccgtggtat gccggatggg
      961 gcgtgagtga cgaccgccac ccaaaaatcg gcagccttat tcaaacccaa cgccgcgccc
     1021 cccgcaactt gctgcaactc ttcgccgcag cctatctgca atacagccgc tacctcaacc
     1081 ccaataccgg cgaagcaggc agcctctttg atgtcatcga ctatctggcg acggtcaaac
     1141 gtaaaaacga caaattgcgt ggcgagttat attgcgtcgg tatgtctttg tggaaacgcg
     1201 cggttgccaa accgttcttt aacgtaccct cttgccgtct gaaatttatc tcttccaccc
     1261 aaaaactggc aagggtcaaa ctgtccgacg atgcacgcat cctggcttgg ggcaacggca
     1321 aagaggccat cgtccgcttt gccgaacaac accacatccc cctgctgcgc atggaagacg
     1381 gctttatccg ctcggtcgga ctcggctcca acttagtgcc gccgctgtcg ctcgttaccg
     1441 acgatatgag catttatttc aatgccgaaa ccccgtcccg tcttgaatac atcctacaaa
     1501 accaaaactt cgacgatcaa gactttcaga cggccttgaa gctgcaaaaa atgctgaccg
     1561 aaaaccacat cagtaaatac aacgtcggca gctcagactt caccgccccg tcaaccgaca
     1621 aaaccgtgat cctcgttccc ggccaggttg aagatgatgc gtctatccgc tacggttcgc
     1681 cccaaatcta ccgcaatctg gatttgctcc gtaccgtacg cgaacgaaac cccaatgcct
     1741 atatcatcta caaaccgcat cccgatgtag tcagcggtaa ccgcatcggc catatttccc
     1801 ctgaagatgc tgcacgatat gccgaccaaa ccgccgaaca agccgacatc ctgacctgtc
     1861 tccaatacgc agacgaaata cataccatga cttcgctgac cggttttgaa gccttgttgc
     1921 gcggcaaaaa agtcagctgc tacggcctgc ctttttacgc aggctggggg cttacccaag
     1981 atctgctccc catcccgcgc cgtagccgca gacttgagct ttggcagctg attgccggca
     2041 cgctcatcca ctatcccgac tacatccacc ccgaaaccca tcaggccata aatgcagaaa
     2101 ccgcagccca aatcctgata cgacaaaaaa atatgcaaaa aaacaacaac ggattacatc
     2161 gcgggtgctt tgccaaaaaa ttaggtaaaa tcaaacaact atatcgatct ttcaaataaa
     2221 taccatcaaa gttaacgatg cgtcataaac ttgcctctat tgcggcatca ttgcctttgc
     2281 atcgttaatt ctcttggcgt atgcttgaaa gttcaaccta aaactattac ataaaaaaca
     2341 aaaccacatt gcaacatgaa acagaccgtc ctcaaaaata acctgcaaaa cctgcttgaa
     2401 agcgcagaaa atatcctgct gcttcaaggc cctgtcggcg atttttttct gcgccttgcc
     2461 gactggctga ctgcaaacgg caaaaccgta cataaattca actttaatgc aggcgacgac
     2521 tatttttatc cgcccactca agcgcatacc gttgttttta acgacaacta cgatgccttt
     2581 cctgagtttt tgcaagaata catcactcaa catcacatcc aggccgttgt ctgctttggc
     2641 gacacacgcc cttatcacgt cattgcaaaa cgcattgcaa acgaaaacca agccagtttc
     2701 tgggcgtttg aagaaggcta tttccgcccc tactacatca ccttagaaaa agacggcgtc
     2761 aacgcatttt ccccgttgcc gcgccgtgcc gacttttttc ttgaacaatt ccctaagctt
     2821 gcccagcaag aatataaagc gccaacgccg gtacacggcg gttttacgcc catggcaaaa
     2881 aacgctatcc gttactatat cgagttgttc cgcaatccac gcaaataccc cgactacatc
     2941 caccaccgcg cacccaatgc cggccattac ctcaaaccgt ggtcgctctc catcctcaag
     3001 cgtttgaact actatattga agacatccaa atcgccaaac gtgtggaagc aggcaaatac
     3061 ggcaagtttt ttattgttcc cttacaggta ttcaacgaca gccaagtccg tatccattgc
     3121 gactttccca gcgtccgcag cttcctgctc catgttttga gttcatttgc cgagcacgcg
     3181 cctgccgata ccaacatcat catcaagcat catccgatgg accgcggttt tatcgactac
     3241 tggcgcgaca ttaaacgctt tatcaaagaa caccccgaac tccaaggccg tgtgatttat
     3301 gtccatgatg tccccctgcc cgttttcctg cgccacggtc tcggcatggt taccatcaac
     3361 agcaccagcg gcctgtccgg actgattcac aatatgccag ttaaggttct cggccgtgcc
     3421 tattatgata ttcccggtat tactgaccaa aataccttgg cagaattttg gaatcatccg
     3481 acaccgcctg acaaagagct gttccatgcc taccgaatgt accacctcaa cgtgacccaa
     3541 attaacggca acttctacag tcaggtcttt tttcctaaca aaaaaacctc cgactcttcc
     3601 actccagcaa cctgacttag caaaaataat tcaggccgtc tgaaaacatt tcagacggcc
     3661 tgaaacaatc aataccttag ctactgccat gtaaataaaa cacaaaaacc tgcatttatc
     3721 attaacaata aattacaaaa acagtataat gaccgagctg tcatgagcgc ataccgactc
     3781 aacctgagcc ctttgtaaca cacaaaatat ggatgtatcc ctaggcaaaa caatataaca
     3841 agccaaacat cctaaagata agccggcaag gcaacacact ctacaaaacc gtgccaagca
     3901 atatttttac aaagccctca accggtatcg ccgcccatat gccgcagcat ccgtcttcca
     3961 ctttatatcc gcccgcaaac catgaccgcc gctcctgata tcctctaccg gcaagccgcc
     4021 gcccttttgg aacaatccaa taccgcccaa gccctgcccc tgctgcaaca ggccgcagag
     4081 cagggatata cggaagctgc ttttgtattg ggcaaccatc tgctgaaaaa cggtcatccg
     4141 gagcaggcac ttccatggtt ggaagccgcc gcggcccaac gccatcccaa agcactcttc
     4201 tccctgctgc aacaacgcga acacaacggc accccgaccg gacagcttct caacgactat
     4261 gcctggctgg gtgagcaggg gcactcagaa gcccaattaa tcctcatgcg ttaccacgcg
     4321 caacgcaacg atccacaatc gctctactgg gcggaacttg ctgccgcccg atatgccgca
     4381 cctgcgtatt accatctggc acgccatcat caacgccaag gcgacgttga aacagccatc
     4441 gaacaatacg aaaaagcggc agcactcggc gtaactgccg cctgctggca acttggtcaa
     4501 atctacttct acggtacagg tgtcagcccc aaccacgcac aagccgaaca ctatctcgaa
     4561 ccagccgcac aagccggcca catcgccgca caaacgctgc tggctgacct tcttgccgcc
     4621 caacgcaaac ctgaagcctt ggaatggtat cgtcgtgccg ccgataagga acaagcggaa
     4681 gcacagtcta agctggccca atacgccctg accggcgaac tttccgaacg cgatccgttc
     4741 caagcggcac gatatgccaa agccgctgcc gagaaaaacc atcctgaagc cctgaaaatc
     4801 atgggcgacc tctaccgcta cggcctcggc atcaaagctg acaaccatat cgcgcacgat
     4861 tactaccacc gtgctgccgc gctgggttct gccgccgcag cacaaaaact catcagcgac
     4921 gccgcgctgt accatccgca acaatacgaa caaatcaaaa ctgccgccct gcaacagcaa
     4981 caaaccgaaa ccatctaccg tttggcggaa acacaagccc atgccatcgg tcgtcccgcc
     5041 gactataatg ccgcgcgaaa aaattacatg gaagctgctg agttccacca taaaaacgcg
     5101 gcggctgcct taggccgcat ctaccattac ggcctcggta cggcgcaaga tcctcgggcg
     5161 gctgcacact ggtacgccat tgctgccgaa caaaaccacc cttccgccca ataccacctc
     5221 gcctgttttt actatcacgg gcaaggtgtc ggctgtcatg ttccgaccgc ctgctactgg
     5281 ctgcaggctg ccatcagcaa cggccacact tcggccgaat cattaatatc cctattagaa
     5341 caatggcgac gcgaagcaca ccatgccatc ggacaaaagg ccgtctgaaa agatttacac
     5401 tcgcattttt tgacaatctt taactattcc cctaatattt gccagttatt tttcacggac
     5461 acgccattgt tttcatttct ttctgaaaac accttgtccg cgcatcaata ccatgacact
     5521 cggcggataa cgccaagcgt tgaaacacac tacatccgga acaaaaacgg atgctcggaa
     5581 aaatatttct aggaggtgaa acaacatgga atgggaattc aacagttatt acacactgat
     5641 tgccgccacg ctcgtgttgc tggttggtaa atttctggtt caaaaaatca aattcttacg
     5701 agacttcaat attcccgagc cggtcgctgg cggtttggtt gccgcgatta tcctgtttgc
     5761 gctgcacgag gcgtacggcg tgagcttcaa atttgagaaa ccgctgcaaa atgcgtttat
     5821 gctgattttt ttcacgtcca tcggcttgag cgcagatttt tcccgtttga aggcgggcgg
     5881 tttgccgctg gtggttttta ccgcgattgt gggcggattt atcttggtgc aaaactttgt
     5941 cggggtcgga ctggctacgg ctttgggttt ggacccgctc atcggtctga ttaccggttc
     6001 ggtgtcgctg acgggcggac acggtacgtc aggtgcgtgg ggacctaatt ttgaaacgca
     6061 atacggcttg gtcggcgcaa ccggtttggg tattgcatcg gctactttcg ggctggtgtt
     6121 cggcggcctg atcggcgggc cggttgcgcg ccgcctgatc aacaaaatgg gccgcaaacc
     6181 ggttgaaaac aaaaaacagg atcaggacga caacgcggac gacgtgttcg agcaggcaaa
     6241 acgcacccgc ctgattacgg cggaatctgc cgttgaaacg cttgccatgt ttgccgcgtg
     6301 tctggcgttt gccgagatta tggacggctt cgacaaagaa tacctgttcg acctgcccaa
     6361 attcgtgtgg tgtctgtttg gcggcgtggt catccgcaac atcctcactg ccgcattcaa
     6421 ggtcaatatg ttcgaccgcg ccatcgatgt gttcggcaat gcttcgcttt cgcttttctt
     6481 ggcaatggcg ttgctgaatt tgaaactgtg ggagctgacc ggtttggcgg ggcctgtaac
     6541 cgtgattctt gccgtacaaa ccgtggtgat ggttttgtac gcgacttttg ttacctatgt
     6601 ctttatgggg cgcgactatg atgcggcagt attggctgcc ggccattgcg gtttcggctt
     6661 gggtgcaacg ccgacggcgg tggcaaatat gcagtccgtc acgcatactt tcggcgcgtc
     6721 gcataaggcg tttttgattg tgcctatggt cggcgcgttc ttcgtcgatt tgattaatgc
     6781 cgcgattctc accggttttg tgaatttctt taaaggctga ttttccgcct ttccgacaaa
     6841 gcacctgcaa ggtttaccgc ctgcaggtgc ttttgctatg atagccgcta tcggtctgca
     6901 ccgtttggaa ggaacatcat gtatcggaaa ctcattgcgc tgccgtttgc cctgctgctt
     6961 gccgcttgcg gcagggaaga accgcccaag gcattggaat gcgccaaccc cgccgtgttg
     7021 caaggcatac gcggcaatat tcaggaaacg ctcacgcagg aagcgcgttc tttcgcgcgc
     7081 gaagacggca ggcagtttgt cgatgccgac aaaattatcg ccgccgccta cggtttggcg
     7141 ttttctttgg aacacgcttc ggaaacgcag gaaggcgggc gcacgttctg tatcgccgat
     7201 ttgaacatta ccgtgccgtc tgaaacgctt gccgatgccg aggcaaacag ctccctgctg
     7261 tatggggaaa cgtcttttgc agacatcgtg cggcagaaga cgggcggcaa tgtcgcgttt
     7321 aaagacggcg tattgacggc agccgtccgc ttcctacccg tcaaagacgg tcagacggca
     7381 tttgtcgaca acacggtcgg tatggcggcg caaacgctgt ctgccgcgtt gctgccttac
     7441 ggcgtgaaga gcatcgtgat gatagacggc aaggcggtaa aaaaagaaga cgcggtcagg
     7501 attttgagcg gaaaagcccg tgaagaagaa ccgtccaaac ccacgcccga agacattttg
     7561 gaacataatg ccgccggagg ggatgcagac gtaccccaag ccgcagaagg cgcgcccgaa
     7621 ccggaaatcc tgcatcctga cgacggcgag cgtgccgata ccgttaccgt atcacggggc
     7681 gaagtggaag aggcgcgcgt acaaaaccag cgtgcggaat ccgaaattac caaactttgg
     7741 ggaggactcg ataccgacgt gcaaaaagag ttggtcggcg aacaacgcaa gtgggcgcag
     7801 gaaaaaatca gcaactgccg acaagccgcc gcgcaggcag accggcagga atacgccgaa
     7861 tacctcaagc tgcaatgcga cacgcggatg acgcgcgaac ggatacagta tcttcgcggc
     7921 tattccatcg attaggggca aaccgatgac taccgtcccc aaaagcagga ttcccgtcaa
     7981 accgctgccc gaaaaaacca cagacgaagc caaagtcgaa aaatggcggc agctcggtgc
     8041 ggaacacggt ttgtcgggcg aatgggcagt tgccgccaga ttgggcgaga acggttttac
     8101 cgaagaacag atggaaaata tcgccaacct gttcggcaga taaagagaaa attgacggaa
     8161 atgccgtctg aaaccctgtt atcggtttca gacggcattt tgaccaatac ggtacgcagg
     8221 cgcaaaacag ccggcttttc ctgtgttacc tatgctgatg tttcaacaca caggacgata
     8281 caaaaaacgt cgccctatgt gccgtcctga ttcggaaggg ttacgctcct tccaaatata
     8341 gtggattaac aaaaaccagt acggcgttgc ctcgccttag ctcaaagaga acgattctct
     8401 aaggtgctga agcaccaagt gaatcggttc cgtactattt gtactgtctg cggttcgccg
     8461 ccttgtcctg atttttgtta atccactata aatcgagcct aaaacaatac ctgtttcaga
     8521 cggcattgtt acattcaaac ggcaggccgt ttatttgaat ttgtaggtgt attgcaggcc
     8581 gatgatgtcg gcgtggtttt tgaaacgtgc ggaagacgcg cctttgctgt ccacatcgtt
     8641 gccgcttgcc ttcgccgtgc ggtaggtggt gtcgttgatg tggatgtggg tgtaggcggc
     8701 atcgacgacg tggtttttgc cgatatgata tttcataccg gcggagaacc agatgcggtt
     8761 gccgtcgggc aggctgttca tgcggtagtc ggcgttgcgg acgggcgatt tgtcaaaagc
     8821 gatgccggcg cgcagttgca gcggttcgct gatttgataa gaaccgccca agccgacttt
     8881 gtaggtgttg cgccagttgg gggtgatggt ggtgcggtcg gattttttgc ccataccgat
     8941 ggttttttct ttttcaaaaa ccagttccgc cttatcgaag cggctgtggc gcgtccaagt
     9001 tacgtcgccg aacaggtcgg ctttgtcgga cactttgtac atgccgtgta cggacaaaga
     9061 ctcaggcgtt acgattttga cgcgggcttt ttcattcgcc gtgtagccgt ttgctgcaag
     9121 catcgtactc cacattgctt tcgccgccgc gtcgtctgcc gcccattcgg catcgccttt
     9181 gagcgtgtgt gagactttgg aacggtagtt cacgcccacg cgcgcacggt cgttgatgtc
     9241 ccacatccac gccagttggt agccgaagcc ccaatcgctg cctttgacat cggcgtgtcc
     9301 gtcggctttg atttgggcgg ctttagttgg attggggggt gtttctttta gtgctttcgc
     9361 tttttccata atcccccagt cggcatattt gcgcagctcg gcggaagtat gttgggcgat
     9421 gatgcctgcg ccgaaggaat ggcggtcgtt gagtttccac gcggcgacag gttcgacggc
     9481 gatgctggtc agaccgagtt tgttgatgtt gtggcgcaac acggaatctt tttcgtattc
     9541 ggtggcggag ccgaagggga cgtacacgcc caagcccacg gtcagattgt cgttgacttt
     9601 gtatgcgccg tagatgtggg gcgcgaccgt ggttttggtg attttgccgc ttttcgaacc
     9661 ttggacggga agcccggtaa agtcggtggc ggaatccgcc tcataatgaa tgctgggcag
     9721 cacgatgttg gcgttgacgg aaatctggct gctgtcgagt ttggtcaggc cggcagggtt
     9781 gtagaagatg gtcgatgcgt cggcggcttc tgcggcggcg gcatttgccg tgctttgcgc
     9841 gttgaccgac tgtgtgccga agtggtagcc ggatgcgtgg acggatgcgg cggcaaaggc
     9901 agtgccgagc agtaggacgg tttttttcag tgcggaaggg gtcatttcgg tttccgtaaa
     9961 aaggcggacg gtggataaat atagtggatt aacaaaaatc aggacaaggc gacgaagccg
    10021 cagacagtac agatagtacg gcaaggcaac gccgtactgg tttaaattta atccactata
    10081 aaaaaggcag tcggaaatgc cttgtttcgc tttagtatag gtactcgatt ttatccgatg
    10141 ttgccggatt tgcacaattt tttcagagtt tgcccgaacc gccgcgccgc cgcaaaaaat
    10201 gccgtctgaa gcctcgggca tcgacttcag acggcatttt ccactcaggg cggattattt
    10261 gacgcgcagc acttccagcg tattggtcga accggattcg cgcatttgcg agccgctggt
    10321 aatgatgtat tggtcgccgg aatgcaggat gttgtgttcc accagcatgg tttcgacttc
    10381 gtttaacgcc gtgtcgtggt cggtgctggt tgccaaaatc agcgggcgca cgccccggta
    10441 catcgccata cggcgttggg cggaaacgct cggggtcagc gcgaaaatcg gcagggtgat
    10501 gttgtggcgg ctgatttcaa aggcggtcga accgctttcg gtcagggcga cgatggcttt
    10561 ggcgtgaacc gcgcgcgcca cgctgaccgc gccgccggca accgccaggt tggtgctgac
    10621 cgcttcggga tactccgcct gttcggcaac gccgttgagc gaatcctgct ctttttccgc
    10681 agccgcgcag ataattgcca tttggctgac ggtttcaaac ggatacgcgc cgacggcggt
    10741 ttcggcggaa cacatcaccg catcggtgcc gtccaatacc gcgtttgcca catcgctgac
    10801 ttccgcgcgg gtcggcacgg ggttggtaat catcgattcc atcatttgcg tcgccgtaat
    10861 gctgaagcgg cgcaactcgc gggcgcggcg gatcatccgt ttttgcaggg cggggacggc
    10921 ggcgtgtccg acttcgaccg ccaagtcgcc gcgcgcaacc ataatgccgt cgccggcaag
    10981 gatgatttcg tccaagtttt caatcgcttc cacgcgttcg attttggaaa ccaaaccggg
    11041 gcgcacggcc gtgctgccct tcatttcttc ttcgactttg gcgcgcgcga tatgcaaatc
    11101 ttcggcggat ttcacaaagc tgatggcgag gtagtcgcaa ccgatggcaa tcgcggtttt
    11161 caggtcgcgg aagtcttttt cggtcaacgc gcctgcggac agaccgccgc cgcgtttgtt
    11221 gatgcccttg ttgcttttca ggacgtggct gttttccacc cttgtgataa tcctgctgcc
    11281 ttcgacggat tccacggtca gggtcagcag gccgtcgtcc agccacaata cgtcgcctgc
    11341 ggcaacgtcg tcgggcaggt cgcggtagtc caaaccgacc gcctcgcgcg tgccttcgcc
    11401 ttcgagcgcg gcatccagta ccagcgtttc gcctttgttc aattcaatgc cgccgccggc
    11461 gattttgccc acgcggattt tcggcccttg caggtcggca atgatggcga tttcctgtcc
    11521 ggcgcgtttt gccgcctcgc gcacgatgcg ggcgttttcc tgatggaatt cgggcgtgcc
    11581 gtggctgaag ttgaagcgga cgacgttcag gccgccgacg cggatcatat cttccaacag
    11641 ttctacgttg ttgctgcccg gcccaagggt ggcgacgatt ttggtgttgt ggctgatgcg
    11701 ggtcagatcg cggcttgtct ggttcatatg aaagtccttt tggtctcaat cgggtgtttt
    11761 gcggtatttt gttacaaaat tacagaaatt tggaaccggt ttgatgtcca tttgatgaac
    11821 gcggcggaat attctgtaaa aatatgattt aaattaatag tttgatattt tacctgcaaa
    11881 ccgccttttt tggtgcaaaa ttacacggtt ttatgacttg ggctaaattt atttt
//
LOCUS       DAWFHY010000066        11928 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.066, whole genome shotgun sequence.
ACCESSION   DAWFHY010000066 DAWFHY010000000
VERSION     DAWFHY010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.066"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            98..814
                     /locus_tag="WGQ68_001534"
     CDS             98..814
                     /locus_tag="WGQ68_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEZ0715312.1"
                     /translation="MNFENDDIIHAPTTSSLILEERHDSELFRVYALILDGITDQVLL
                     PGKKLTESELCRQMVCSRNTVRGALSLLAHDKIVDLQPNRGAFVHVPDLKEMQDVFNA
                     RIEMETMILNILAGLPDLETRLKPLYAMIRREEEASGRGDRVGWNRLSNAFHVELARL
                     VGNDVLFDIMNTLCARSSLIVAVAGVHREEKHAINTHTHSEHREILDLLLAGRRNRVV
                     KILRRHLGNCMERLEKTLED"
     gene            963..1865
                     /locus_tag="WGQ68_001535"
     CDS             963..1865
                     /locus_tag="WGQ68_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine exporter LysO family protein"
                     /protein_id="HEZ0715313.1"
                     /translation="MDSLMTLLSVLVPMFAGFFIRVPKPYLPALDKVLSVLVYAVLLL
                     IGVSLSRVEDLGSRLDDMALTVLWLFVCTVGANLLALAVLGKLFPWRIKGKGKGVSVG
                     VSGSVGQLGCVLLGFASGKLMRDIWMPSESAGMYCLMLLVFLIGVQLKSSGVSLRQVL
                     VNRRGIRLSVWFMLSSLSGGLLFAASTDGVSWTKGLAMASGFGWYSLSGLVMTEAYGA
                     VWGSIMLLNDLARELFALAFIPLLMKRFPDAAVGVGGATSMDFTLPVIQGAGGLEVVP
                     VAVSFGVVVNIAAPFLMVVFSALG"
     gene            1908..2681
                     /locus_tag="WGQ68_001536"
     CDS             1908..2681
                     /locus_tag="WGQ68_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEZ0715314.1"
                     /translation="MKPKIQRHGEILSLVRRHQFMSVDELAAALDVTPQTIRRDIREL
                     EEAGSLKRHHGGASSGGNLPEGLPADRQIRCQNEKNAIARLIAEHIPDGSSLFVSIGT
                     TMEAVVSELVRRRGSLRVITNNIHAASIASARTDYTVIITSGVVRPLDGGITGVATVD
                     FINQFKVDYAVMSTHGVESDGSLLDDDYKEVSVMQAMIANARVRFLGVDHSKFRSNAL
                     VRLGDITAFDKVFTDRLPDTAMQKMLKEAGVECLIADAV"
     gene            2793..4481
                     /locus_tag="WGQ68_001537"
     CDS             2793..4481
                     /locus_tag="WGQ68_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HEZ0715315.1"
                     /translation="MLNKDQFADNHFIRTIIEDDLKSGKHEAVQTRFPPEPNGYLHIG
                     HAKSICLNFGLAYIYDGLCNLRFDDTNPEKENDEYVNAIKEDVEWLGFHWAGEPRFAS
                     NYFDQLYDYAVGLIKDGKAYVDDLTLEEMREYRGTLTEAGKNSPYRDRSIEENLDLFT
                     RMKNGEFPDGSKTLRLKIDMASGNINMRDPVIYRIRRAHHHNTGDKWCIYPMYDYTHC
                     ISDAIEGITHSLCTLEFEAHRPLYDWVLDNIHAPHATRPRQYEFSRLELLYTITSKRK
                     LNQLVVEKHVSGWDDPRMPTISGMRRRGYTPEGLRLFAKRAGISKSENIVDMSVLEGA
                     IREELENSAPRLMAVLNPLKVTLTNFEAGKTQSRRAAFHPNHEEMGEREVPISQTIYI
                     EADDFAENPPKGFKRLIPGGEVRLRHGYVIKCDEVVKDEAGNVVELKCSIDHDTLGKN
                     PEGRKVKGVIHWVSAEHAAEIKVRLYDRLFTVERPDAVRGEDGEYLLFTDFLNPESVK
                     EITAYAEPAAKDLPAESRWQFERIGYFVTDRKDHGKDTPVFNRTVTLKDSWQPK"
     gene            4574..5530
                     /gene="gshB"
                     /locus_tag="WGQ68_001538"
     CDS             4574..5530
                     /gene="gshB"
                     /locus_tag="WGQ68_001538"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEZ0715316.1"
                     /translation="MKVLFIADPMASFKTYKDTTYAMMREMAKRGWRLFHTLSSGLSV
                     KNGLVVADASAFEFLGAQDDHDKNWFAAADKVQTALKAFDAVIMRTDPPFDMQYLYAT
                     QLLTLAEQQGAKVFNSGQSMRDFNEKLAILNFSRFTAPTLVTTRSADVRAFLKEHGDI
                     IIKPLDGMGGMGIFRLTEKDPNIGSILETLMRLDSRTIMAQRYIPEIVHGDKRILIIG
                     GEVVPYALARIPQNGETRGNLAAGGRGVAQELDGRDREIAETLAPELKRRGILLAGLD
                     VIGSNLTEVNVTSPTGFQEIMKQKGFDVAAMFADAVAAWSVR"
     gene            5842..6225
                     /locus_tag="WGQ68_001539"
     CDS             5842..6225
                     /locus_tag="WGQ68_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEZ0715317.1"
                     /translation="MEPSSYAAEKKGKGGIRRVINAFGYSIDGIAAAYRYEAAFRQVL
                     WLNVLLVCAAFFWVSETAVRLPLIIASFVSVIVELFNTAVEAAVDHTSTEKHELAKRA
                     KDAGSAAQLFAMLMLAAVWLSALFG"
     gene            6340..6813
                     /locus_tag="WGQ68_001540"
     CDS             6340..6813
                     /locus_tag="WGQ68_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HEZ0715318.1"
                     /translation="MKKLLAAVMMAGLAGAVSAAGVHVEDGWARTTVEGMKIGGAFMK
                     IHNDEAKQDFLLGGSSPVADRVEVHTHINDNGVMRMREVKGGVPLEAKSVTELKPGSY
                     HVMFMGLKKQLKEGDKIPVTLKFKNAKAQTVQLEVKIAPMSAMDHGHHHGEAHQH"
     gene            6939..8042
                     /gene="mnmA"
                     /locus_tag="WGQ68_001541"
     CDS             6939..8042
                     /gene="mnmA"
                     /locus_tag="WGQ68_001541"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEZ0715319.1"
                     /translation="MNTTANPSNIIVGLSGGVDSSVTAALLKQQGYQVRGVFMQNWED
                     DDNDEYCSIKQDSFDAIAVADIIGIDIDIVNFAAQYKDKVFAYFLQEYSAGRTPNPDV
                     LCNAEIKFKCFLDYAVGQGADTIATGHYARKEVRNGVHYLLKGLDRNKDQSYFLYRLK
                     PFQLERAIFPLGGLEKPEVRRLAAEFKLPTAAKKDSTGICFIGERPFREFLQKYLPTD
                     NGKMVTPEGKTVGEHVGLMFYTLGQRKGLGIGGGGEPWFVAAKDLTKNELIVVQGHDH
                     PLLYTRSLVMNDLSFTLPERPKAGRYTCKTRYRMADAPCELRYLDEETAELVFDEPQW
                     AVTPGQSAVLYDGDICLGGGIIQSTDKPVIITR"
     gene            8113..9783
                     /locus_tag="WGQ68_001542"
     CDS             8113..9783
                     /locus_tag="WGQ68_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEZ0715320.1"
                     /translation="MEKIWLDSYEKGVSAEIDITQYNSVSDVFRQSVEKFADQPAFQN
                     MGKTLTYAETGKLATDFASYLQNVLKLPRGERVAIMLPNVLQYPVALFGILQAGLVAV
                     NTNPLYTPRELEHQLKDSGATAIIVLENFANTLELVLPRTQIKHVIVASVGEMFGLLK
                     GSLINFIIRKIKKMVPEYRIRETVSFQTALKEGAKHVFQPVALNREDTALLQYTGGTT
                     GVAKGAVLSHGNICANMLQAKEWIKNQLREGKETVIAALPLYHIFALTVNLMIFANAG
                     SKIILITNPRDMKGFIGELKKQRINVFIGVNTLFNAMVNRPDFAEVDFSELRLTLGGG
                     MATQKAVAEKWKKITGTPIVEAYGLTEASPGVCCNPLNIESYSGSIGLPVSSTEVELR
                     DANGKEVPVGQPGELWVKGPQVMQGYWNRPEETAKAIDARGFLETGDIAVMDEKGWLK
                     LVDRKKDLIVVSGFNVYPNEIEEVIAHHGKVMEVACIGVPDEKTGEALKVFVVRKDPS
                     LTKEELIAFCRTELTAYKVPKDIEFRDELPKSNVGKILRRELRQSTGK"
     gene            9895..11529
                     /locus_tag="WGQ68_001543"
     CDS             9895..11529
                     /locus_tag="WGQ68_001543"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEZ0715321.1"
                     /translation="MTKFIFVTGGVVSSLGKGIAAASIAAILESRGLNVTMLKLDPYI
                     NVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDSTMTRRNSFSTGQVYENVIAKE
                     RRGDYLGGTVQVIPHITDEIKRRIHEGAAGYDVAIVEIGGTVGDIESLPFLEAIRQMR
                     SQLGRNNTLFAHLSYVPYIAAAGEIKTKPTQHTVKEMLSIGLQPDILICRMDREMPAD
                     ERRKIALFCNVEERAIVGSYDVDSIYECPEMLHDQGIDNIITEQLQLNVQQADLTAWK
                     KIVHAVKNPKHTVKIAMVGKYVDLTESYKSLIEALKHAGIHTETDVQITFVDSESIEK
                     NKGDVSMLKDMDAILVPGGFGSRGVEGKIAAVRYARENNVPYLGICLGMQIALIEYAR
                     DVAGLKGANSTEFDLKCAAPVVALIDEWQTADGSVETRDESADLGGTMRLGAQEVELK
                     AGSLAAKIYGSGHIRERHRHRYEVNNNYVPQLEKAGLVIGGVSAGRERLVETIELPNH
                     PWFFACQFHPEFTSNPRKGHPLFTAFVKAALNNKKA"
     gene            11756..>11928
                     /locus_tag="WGQ68_001544"
     CDS             11756..>11928
                     /locus_tag="WGQ68_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0715322.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDRVIDWQPIEQYLNRQKTR
                     YLRDHRGRPAYPL"
BASE COUNT         2964 a         3217 c         3143 g         2604 t
ORIGIN      
        1 aatttgtgca atgctttaat tcctctgaca cgatttgaag cacagccgcc ttaaaacatc
       61 ttaaaaaatt tgaaagcgta aaaatacgga aaacatcatg aactttgaaa acgacgacat
      121 tatccatgcg ccgaccacgt cttccctgat tctcgaagag cggcacgatt cggagctgtt
      181 ccgtgtttac gccctgattt tggacggcat taccgatcag gtgctgctgc ccggcaaaaa
      241 gctgaccgag tccgaacttt gccgccagat ggtgtgttcg cgcaacaccg tccgcggcgc
      301 gctgtcgctt ttggcgcacg acaagattgt cgatttgcaa cccaacaggg gcgcgttcgt
      361 ccacgttccc gatttgaaag aaatgcagga tgtgttcaat gcgcgcatcg aaatggagac
      421 gatgattttg aatatcctcg ccggcctgcc ggatttggaa acgcgcctca agccgcttta
      481 tgcgatgata aggcgcgaag aagaggcttc cggcaggggc gaccgcgtcg gctggaaccg
      541 cctgtccaat gccttccacg tcgaactggc gcgcctggtg ggcaacgatg tgctgttcga
      601 cattatgaac acgctgtgcg cgcggtcttc cctgattgtc gccgtggcgg gcgtgcatcg
      661 cgaggaaaaa cacgccatca atacgcatac gcattccgaa caccgcgaaa tcctcgacct
      721 gctgctggcg ggcaggcgca accgcgtggt caaaatcctg cgccgccatt tgggcaactg
      781 tatggaacgc ttggaaaaga ctttggaaga ttgaatgccg gagcggataa gccgcctgaa
      841 ccttcaggcg gttttttaat ggcgaacctg atgccgtctg aaatatggat gcgggtatct
      901 gcaattttca gacggcaatt tttaaaccgc atatatcatg cggcaataaa ggagggcagt
      961 agatggacag cctgatgaca ttgctttcgg tattggtacc gatgttcgcc ggatttttta
     1021 tccgtgtgcc caagccttac ctgcccgctt tggataaggt gctatcggtt ttggtgtatg
     1081 ccgtgctgct gctgatcggc gtatcgttgt cgcgcgtgga ggatttgggt tcgcggttgg
     1141 acgatatggc gttgacggtt ctgtggctgt ttgtttgtac ggtcggggcg aacctgcttg
     1201 ctttggcagt gttgggaaag ttattcccgt ggcggataaa ggggaaaggg aagggcgttt
     1261 cggtcggtgt gtcgggcagt gtggggcagc tcggatgcgt gctgctcgga tttgcatccg
     1321 gcaaactgat gcgcgatatt tggatgccgt ctgaaagcgc gggcatgtat tgtctgatgc
     1381 tgctggtgtt cctcatcggc gtacagctca aaagcagcgg cgtatcgttg cggcaggttt
     1441 tggtcaaccg caggggtatt cggttgtcgg tctggtttat gctttcatct ctttcgggcg
     1501 ggctgctgtt tgccgcatcg acagacggtg tgtcgtggac gaaaggtttg gcgatggctt
     1561 ccggcttcgg ttggtattcc ctctcgggtt tggtcatgac cgaggcttac ggcgcggtat
     1621 ggggcagcat catgctgctg aacgatttgg cacgagagct gtttgcactg gcatttatcc
     1681 cgctgctgat gaagcgtttt ccggatgcgg cggtgggggt tggtggtgcg accagtatgg
     1741 atttcacatt gcccgtgatt cagggtgcgg gcggtttgga agtcgtgccg gtagcggtca
     1801 gcttcggcgt ggtggtcaat atcgccgccc catttctgat ggtggtgttt tccgctttgg
     1861 gttgaacgcg gtaaaatcgg catcccgatg caaggaggca gaaaacgatg aaaccgaaaa
     1921 tccaaaggca cggagagatt ttaagccttg tccgccggca tcagtttatg tcggtggacg
     1981 agcttgccgc cgcattggac gttaccccgc agacgatacg ccgcgacatc cgcgagttgg
     2041 aggaggccgg cagcctgaaa cgccatcacg gcggcgcatc ttcgggcgga aacttgccgg
     2101 agggcctgcc cgccgaccgc caaatccggt gtcaaaacga aaaaaacgcc attgctcggc
     2161 tgattgcgga acacatcccc gacggttcgt cgctgtttgt cagtatcggc acgacgatgg
     2221 aggccgtggt gtcggagctg gtgaggcggc gcggcagcct gagggtgatt accaacaata
     2281 tccacgccgc ctcgattgct tcggcgcgga cggattatac ggtcattatc acttccggag
     2341 tcgtccgtcc tttggacggc ggtattaccg gcgtggcaac cgtcgatttt atcaaccagt
     2401 tcaaagtcga ttatgccgtg atgagtacgc acggcgtgga aagcgacggt tcgcttttgg
     2461 acgacgacta caaagaagtc agcgtgatgc aggcgatgat tgccaacgcg cgcgtccgct
     2521 tcctcggcgt ggaccacagc aaattccgca gcaacgcgct ggtcaggctt ggcgacatta
     2581 cggcgtttga caaagtattt accgaccggc tgcctgatac cgcgatgcag aagatgttga
     2641 aagaggcggg ggtggaatgc ctgattgccg atgccgtctg aacgctatgt caaagcgcgc
     2701 aagtcgggta caataaacac atcatcaaac cgcttcagac ggcataagga accgccccaa
     2761 tgccgtctga agccatctgt tcaaagaaaa ctatgctcaa taaagaccaa tttgcggaca
     2821 accattttat ccgcaccatc atcgaagacg acctcaaaag cggcaaacac gaagccgtcc
     2881 aaacccgttt tccgccggag cccaacggct acctgcacat cggacacgcc aaatccatct
     2941 gcctgaactt cggtttggcg tatatttatg acggcttgtg caacctgcgt ttcgacgata
     3001 ccaatcctga aaaagaaaac gacgaatacg tcaacgccat caaagaagac gttgagtggt
     3061 tgggtttcca ttgggcaggt gagccgcgtt ttgcctccaa ctatttcgac cagctttatg
     3121 actacgccgt cggtttaatc aaagacggca aggcgtatgt cgatgatttg acgctcgaag
     3181 aaatgcgcga ataccgaggc acactgaccg aagcaggcaa aaacagccct taccgcgacc
     3241 gcagtatcga agaaaacctc gacctgttca cacgcatgaa aaacggcgag tttcccgacg
     3301 gcagcaaaac cctgcgcctg aaaatcgaca tggcatccgg caacatcaat atgcgcgacc
     3361 ccgtcatcta ccgcatccgc cgcgcccatc accacaacac cggcgacaaa tggtgtatct
     3421 acccgatgta cgactacacg cattgcatct ccgatgccat cgaaggcatc acgcattcct
     3481 tgtgtacgct cgaattcgaa gcacaccgtc cgctgtacga ctgggtgttg gataacatcc
     3541 acgcgccgca tgccacccgt ccgcgccaat acgagttttc ccgtttggag cttttgtaca
     3601 ccattacctc caaacggaaa ttgaatcagt tggttgtgga aaaacacgtt tccggctggg
     3661 acgatccgcg tatgccgacc atttccggta tgcgccgtcg cggctacacg cccgaaggcc
     3721 tgcgtctgtt tgccaaacgc gccggtattt ccaaatctga aaacatcgtc gatatgagcg
     3781 tgttggaagg tgcgattcgc gaagagctgg aaaactccgc cccgcgcctg atggcggtgt
     3841 tgaacccgct caaagtaacc ctgaccaact ttgaagccgg caaaacccaa agccgccgtg
     3901 ccgcgttcca tccgaaccac gaggaaatgg gcgagcgcga agtacctatt tcacaaacca
     3961 tctatattga agccgacgac tttgccgaaa atccgcctaa aggcttcaaa cgcctgattc
     4021 ccggcggcga agtgcgtttg cgccacggct atgtcatcaa gtgcgatgaa gtcgtcaaag
     4081 acgaagcagg caatgtggtt gaactcaagt gcagcatcga ccacgatacc ttgggcaaaa
     4141 atccagaagg ccgcaaagtt aaaggcgtga ttcactgggt gtctgccgaa cacgccgccg
     4201 aaatcaaagt ccgcctgtac gaccgcctct ttaccgtcga gcgtcccgat gccgtgcgcg
     4261 gcgaagatgg cgaatacctg ctgttcaccg atttcctcaa tccagaatcc gtcaaggaaa
     4321 tcaccgccta cgccgaacct gccgccaaag atttgccggc agaaagccgt tggcagttcg
     4381 agcgcatcgg ctacttcgta accgaccgca aagaccacgg caaagacacg ccggtgttta
     4441 accgcacggt cacgctgaaa gactcttggc agcctaagta aaactccatc cttgccgtct
     4501 gaatattgtt cggacggcat ttcccctttg cccgcgaaat gcggcacatt cggcacaccg
     4561 gaaaggaaat atgatgaaag tcctctttat cgccgacccg atggcaagtt tcaaaactta
     4621 caaagacacc acctacgcaa tgatgcggga aatggcaaaa cgcggctggc ggctgtttca
     4681 taccttgagc agcggattgt cggttaaaaa cggtctggtg gttgctgatg cgtctgcttt
     4741 tgagtttctc ggcgcacaag acgaccacga caaaaactgg tttgccgccg ccgacaaagt
     4801 tcagacggca ttgaaagcat ttgatgccgt gattatgcgt accgatccgc cgttcgatat
     4861 gcaatacctc tacgccaccc aattactgac gctggcggaa cagcagggcg cgaaagtctt
     4921 taacagcgga cagtcgatgc gtgacttcaa tgaaaaactg gcgattttga acttcagccg
     4981 ctttaccgcg cccacgctgg taacgacccg ttccgccgat gtccgcgcat ttttgaaaga
     5041 acacggcgac attatcatca aaccgctgga cggtatgggc ggtatgggta ttttccgcct
     5101 gaccgaaaaa gaccccaaca tcggcagcat cctcgaaacc ctgatgcggc ttgattcccg
     5161 caccattatg gcgcaacgct acattcccga aatcgtacac ggtgacaaac gcatcctgat
     5221 tatcggcggc gaagtcgtcc cctatgcttt ggcgcgtatc ccgcaaaacg gcgaaacacg
     5281 cggcaacctt gcggcaggcg gacgcggcgt ggcgcaggaa ttggacggac gcgaccgcga
     5341 aattgccgaa acgctcgccc ccgaactcaa acggcgcggc atcctgcttg ccggtttgga
     5401 cgttatcggc agcaacctga ccgaagtcaa cgtaaccagc ccgaccggat tccaagaaat
     5461 catgaaacaa aaaggtttcg atgtggcggc aatgtttgcc gatgccgttg ccgcgtggtc
     5521 ggtacgttaa accgatgtcg tctgaaaggc ttttgcttca caaccgtttg gtttgttcgg
     5581 gcaggcaggg ttttccctca gccggcagag atacgcttaa tgccgtacgg acacaccgcc
     5641 gccccgtttt cagacggcat atatagtgga ttaacaaaaa tcaggacaag gcggcgaagc
     5701 tgcagacagt acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc
     5761 gttctctttg agctaaggcg aggcaacgcc gtacaggttt ttgttaatcc actatattga
     5821 gaacattttg aaaggatacc gatggaacct tcctcctacg cggcagaaaa aaaaggcaaa
     5881 ggcggcatca ggcgcgtcat taacgcattc ggctattcga tagacggcat cgccgccgcc
     5941 taccgttacg aagcggcatt ccgtcaggtt ttgtggctga acgtactgct ggtgtgcgcg
     6001 gcattttttt gggtttccga aaccgccgtc cgcctgccgt tgattatcgc gtcttttgtg
     6061 tcggtcattg tcgaactgtt caacaccgcc gtcgaagctg ccgtcgatca tacttcgacc
     6121 gaaaaacacg agctggccaa acgcgccaaa gacgcaggtt ctgccgcaca attgttcgcg
     6181 atgctgatgt tggcggcggt ttggctgtct gccctgttcg ggtaaaacgc ttgcagcagg
     6241 atttgtaatc ctttaggatt agtatgtggt atttattatt cacttaatcc actataagat
     6301 taagcacatt ccccgtctga tattataagg agcagaaaga tgaaaaaatt attggcggcc
     6361 gtgatgatgg caggtttggc aggcgcggtt tccgccgccg gagtccacgt tgaggacggc
     6421 tgggcgcgca ccaccgtcga aggtatgaaa ataggcggcg cgttcatgaa aatccacaac
     6481 gacgaagcca aacaagactt tttgctcggc ggaagcagcc ccgttgccga ccgcgtcgaa
     6541 gtgcataccc acatcaacga caacggcgtg atgcggatgc gcgaagtcaa aggcggcgtg
     6601 cctttggagg cgaaatccgt taccgaactc aagcccggca gctatcacgt gatgtttatg
     6661 ggtttgaaaa aacaattaaa agagggcgac aagattcccg ttaccctgaa atttaaaaac
     6721 gccaaagcgc aaaccgtcca actggaagtc aaaatcgcgc cgatgtcggc aatggaccac
     6781 ggtcatcacc acggcgaagc gcatcagcac taatctgctg aaaatatttg aaatgccgtc
     6841 tgaaaaagcc cgggctttca gacggcattt ttatgaccgc ctttaaaatg tgttaaaatc
     6901 tgcctttaaa aaccgccgtt tccaaccata ttctacgcat gaatacgaca gcaaaccctt
     6961 ccaatatcat cgtcgggctc tccggcggtg tcgattcttc cgtaaccgcc gccctgctca
     7021 agcagcaggg ttatcaagtg cgcggtgtgt tcatgcaaaa ctgggaagac gacgacaacg
     7081 acgaatattg cagcatcaaa caggattcgt tcgatgccat cgccgttgcc gatattatcg
     7141 gcatcgatat cgacatcgtt aatttcgccg cgcaatataa agacaaagtc tttgcttatt
     7201 ttcttcagga atacagtgcg gggcgcacgc cgaatcccga tgtgttgtgc aacgccgaaa
     7261 tcaaattcaa atgctttttg gattacgccg tagggcaggg cgcggatacc atcgccaccg
     7321 gacactatgc gcgcaaagaa gtccgcaacg gcgtgcatta cctgctcaaa ggtttggatc
     7381 gaaacaaaga ccaaagctat ttcctctacc gcctcaagcc tttccaactc gagcgcgcga
     7441 tttttccgtt gggcggtttg gaaaaacccg aagtccgccg ccttgctgcc gagttcaaac
     7501 tgccaaccgc cgccaaaaaa gacagcacgg gcatctgctt catcggcgaa cgcccgtttc
     7561 gcgagtttct gcagaaatac ctgccgaccg acaacggcaa aatggttacg cccgaaggga
     7621 aaaccgtcgg cgaacacgtc gggctgatgt tttacacatt gggtcagcgc aaaggtttgg
     7681 gcatcggcgg cgggggagaa ccgtggtttg ttgcggctaa agatttgacg aaaaacgaac
     7741 tgattgtcgt gcaaggacac gaccatccgc tgctctatac ccgcagcctc gtcatgaatg
     7801 atttgagttt cacgcttccc gaacgtccga aggcagggcg ttatacctgt aaaacgcgtt
     7861 accgcatggc ggacgcgcct tgtgagttgc gctatttgga tgaagaaacc gccgagctgg
     7921 tgtttgacga accgcaatgg gcggttacgc cgggccagtc cgccgtgctg tacgacggcg
     7981 acatctgttt gggcggcggc atcattcaat cgaccgacaa acccgtcatc atcacacgat
     8041 aaaggtaatg ccgtctgaaa cggttttcag acggcattgt tccgctcaat tccactttaa
     8101 agaccgatac ctatggaaaa aatctggtta gacagctacg agaagggcgt cagtgccgaa
     8161 atcgacatca cgcaatacaa ttccgtcagc gacgtattcc gccaaagcgt ggaaaaattt
     8221 gccgaccaac ctgcttttca aaacatgggt aagacgctga cttatgccga aaccggcaaa
     8281 ctggcaacag attttgcttc ttatctgcaa aacgtcctta aattgcctcg aggagagcgc
     8341 gtggcaataa tgttgcccaa tgtactgcaa tatccggttg cccttttcgg cattttgcag
     8401 gcaggtttgg tagcggtgaa caccaatccg ctctataccc cgcgcgagct ggagcatcag
     8461 ttgaaagaca gcggcgcgac cgccatcatc gttttggaaa atttcgccaa cacgctggag
     8521 ctggtgctgc cgcgcacgca gatcaaacac gtcatcgtcg cctccgtcgg cgaaatgttc
     8581 gggctgctta aaggttcgct gatcaatttc atcatccgga aaatcaagaa aatggtgccc
     8641 gaataccgta ttcgggaaac cgtttccttt cagacggcat tgaaagaggg ggcgaagcac
     8701 gttttccaac ctgtcgcatt aaaccgcgaa gataccgcgc tgttgcaata cacgggcggc
     8761 acgacaggtg ttgccaaagg ggcggtgctg agtcacggca acatctgcgc caatatgctt
     8821 caggcaaaag aatggattaa aaaccagttg cgcgagggca aagaaaccgt tatcgccgcc
     8881 ctgccgctgt atcatatttt cgccttaacc gtgaatctga tgatttttgc caatgccggc
     8941 tcgaaaatca tcctgattac caacccgcgt gatatgaaag gctttatcgg agaactgaaa
     9001 aaacagcgga ttaatgtatt tatcggcgta aacacactgt ttaacgcgat ggttaaccgt
     9061 cctgattttg ccgaagtcga tttttcagaa ttgcggctga ctttgggcgg cggtatggcg
     9121 acccaaaaag ccgttgccga aaaatggaaa aaaattaccg gtacgcctat tgtcgaagcc
     9181 tacggtctga ccgaagccag cccaggcgtg tgctgcaacc ccttaaacat cgaatcatac
     9241 agcggcagca tcggtttgcc cgtctcgtcc accgaagtcg aactgcgcga cgcaaacggc
     9301 aaagaagtcc ccgtcgggca gccgggcgaa ttgtgggtaa aaggccctca agtgatgcaa
     9361 ggctactgga accgtcccga agaaaccgct aaagccatag acgcgcgcgg tttcttggaa
     9421 accggcgata ttgccgtcat ggacgaaaaa ggctggttga agctggttga ccgtaagaaa
     9481 gacctcattg ttgtttccgg attcaatgtt tatccgaacg aaatcgaaga agtcatcgcg
     9541 catcacggca aagtgatgga agttgcctgt atcggcgttc ccgacgaaaa aaccggcgag
     9601 gcactcaaag tattcgtcgt cagaaaagac ccgtctttga cgaaagaaga acttatcgcc
     9661 ttttgccgca ccgaattaac cgcatataaa gtaccgaaag atatcgaatt ccgcgacgag
     9721 ttgcccaagt ccaacgtcgg caaaatcctg cgccgcgagt tgcggcaaag taccgggaaa
     9781 taaagaaaag atgccgtctg aaaacagccg tccaccgttc agacggcatc cgtccgtttg
     9841 ccagaaccgc gcgcttcacg ttaaaatcac gcattccaat atgggtattc catcatgacc
     9901 aaattcattt tcgtaaccgg cggcgttgtc tcctcactgg gtaaaggtat cgccgccgct
     9961 tctattgccg ccatcctcga atcgcgcggc ttgaacgtta ccatgctcaa gctcgatcct
    10021 tatatcaacg tcgatcccgg cacgatgagc ccgttccaac acggcgaagt gttcgtaacc
    10081 gacgacggcg cggaaaccga cctcgacttg ggacactacg aacgctttat cgattccacg
    10141 atgacccgcc gcaacagctt cagcacgggg caggtgtacg aaaacgtcat cgccaaagaa
    10201 cgacggggcg actatttggg cggcacggtt caagtcattc cgcacattac cgacgaaatc
    10261 aaacgccgca tccacgaagg cgcggcaggt tacgatgtgg cgattgtcga aatcggcggt
    10321 acagtcggcg atatcgaatc tttgccattc ttggaagcca tccgccaaat gcgcagccag
    10381 ctcggccgca acaatacatt gtttgcccac ctgagctatg ttccttacat tgccgccgcc
    10441 ggtgaaatca aaaccaaacc gacccagcat accgttaagg aaatgttgag cattggtttg
    10501 caaccggaca tcctgatctg ccgtatggac agagaaatgc ctgcggacga gcgccgcaaa
    10561 atcgccttgt tctgcaacgt ggaagaacgc gcgattgtgg gcagctacga cgtggacagc
    10621 atctacgaat gccccgaaat gctgcacgac caaggcatag acaacatcat taccgaacag
    10681 ttgcagctta acgtacaaca ggcggatttg actgcgtgga aaaaaatcgt ccacgccgtc
    10741 aaaaatccga aacacaccgt caaaatcgcg atggtcggca aatacgtcga tttgaccgag
    10801 tcctacaaat cattgattga agccttgaaa cacgcgggta tccacaccga aaccgacgtg
    10861 caaattacct ttgtcgacag cgaaagcatc gagaaaaaca aaggcgacgt ttccatgctc
    10921 aaagatatgg atgccatcct cgtccccggc ggtttcggtt cgcgcggcgt ggaaggcaaa
    10981 atcgccgccg tgcgctacgc ccgcgaaaac aacgtgccat acttgggcat ctgcctcggt
    11041 atgcagattg cgctgatcga atacgcccgc gacgtggcag gtttgaaagg tgcgaattcc
    11101 accgagttcg acctcaaatg cgccgccccc gtcgttgccc tgattgacga atggcaaact
    11161 gccgacggca gcgtcgaaac ccgagacgag tccgccgatt tgggcggcac gatgcgtttg
    11221 ggcgcgcaag aagtcgaatt gaaagcaggc agcctcgccg ccaaaatcta cggcagcgga
    11281 cacatccgcg aacgccaccg ccaccgctac gaagtcaaca acaactatgt tcctcagttg
    11341 gaaaaagcag gcttggttat cggcggcgta tccgccggac gcgaacgctt ggtcgaaacc
    11401 atcgaactgc cgaaccatcc ttggttcttc gcctgccagt tccaccccga gtttacctcc
    11461 aatccgcgca aagggcatcc tttgttcacc gcgtttgtca aagctgcgtt gaacaataaa
    11521 aaagcctgat aaagcgttac ttgatgatca aaatgccgtc tgaaagcctg acaaagacct
    11581 tcagacggca ttttcgcaag tcggggatta taaaagccat caatttaatt tttatggttt
    11641 tatctaatat ttaccaactg aatattgtta agcggggcgt gaaactcaag ttgagacctt
    11701 tgcaaaattc cccaaaatcc cctaaattcc caccaagaca tttaggggat ttctcatgag
    11761 caccttcttc cagcaaaccg cccaagccat gattgccaaa cacatcgacc gcttcccgct
    11821 attgaagttg gaccgggtga ttgattggca gccgatcgaa cagtacctga atcgtcaaaa
    11881 aacccgttac ctcagagacc accgcggccg tcctgcctat cccctgtt
//
LOCUS       DAWFHY010000067        11370 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.067, whole genome shotgun sequence.
ACCESSION   DAWFHY010000067 DAWFHY010000000
VERSION     DAWFHY010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11370
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.067"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(239..754)
                     /locus_tag="WGQ68_001545"
                     /pseudo
     CDS             complement(239..754)
                     /locus_tag="WGQ68_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1762..2532)
                     /gene="xth"
                     /locus_tag="WGQ68_001546"
     CDS             complement(1762..2532)
                     /gene="xth"
                     /locus_tag="WGQ68_001546"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0715323.1"
                     /translation="MKITTWNVNSLNVRLPQVQNLLADNPPDILVLQELKLDQDKFPA
                     AALQMMGWHCVWSGQKTYNGVAIVSRNAPEDVHIGLPALPDDPQRRVIAATVGGVRVI
                     NVYCVNGEALDSPKFKYKEQWFAALTEFVRDEMTRYGKLVLLGDFNIAPADADCYDPE
                     KWHEKIHCSSVERQWFQNLLDLGLTDSLRQVHPEGAFYTWFDYRGAMFQRKLGLRIDH
                     ILVSPAMAAALKDVRVDLETRALERPSDHAPVTAEFDW"
     gene            complement(2586..2873)
                     /locus_tag="WGQ68_001547"
     CDS             complement(2586..2873)
                     /locus_tag="WGQ68_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0715324.1"
                     /translation="MNTIPLHTILKLMAHPERMAILIQLLDSERNIAELAKSLSLPAT
                     AVSGHLNRLRAGGLVDFTRYHRIIEYRLISEDAATILRTIRDLENKRAVWC"
     gene            2975..3208
                     /locus_tag="WGQ68_001548"
     CDS             2975..3208
                     /locus_tag="WGQ68_001548"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715325.1"
                     /translation="MDDCTGLYVPFPTFFANTRKGGLSDGIKLPPLPCLTLLPNGIIR
                     IVQKMPYNRRTSCPADASVPSGKQNTCLKRCFF"
     gene            complement(3341..4222)
                     /gene="nadC"
                     /locus_tag="WGQ68_001549"
     CDS             complement(3341..4222)
                     /gene="nadC"
                     /locus_tag="WGQ68_001549"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEZ0715326.1"
                     /translation="MPSENTLFPLPDTLLRPMVEQALSEDLGRRGDITSAAVIAPDKT
                     AKLFLVSREDGVIAGMDLARLAFQTMDPSVRFQAEIRDGQIVRAGQTLAAVEGNARAL
                     LAAERTALNYLTHLSGIATATARAVAEVAEYGTDIVCSRKTLPLLRVLQKYAVRAGGG
                     VNHRMGLDDAVLIKDNHLAYCGSIAQAVRQAKQAVGALTCVEIEVDTLAQLDEAIAAG
                     AERILLDNMDDETLKEAANRCHTQTAHPHTVYCEASGGIGFDRLKRVAQTGVDGIALG
                     YLTHSSRSLDIGLDFLA"
     gene            complement(4298..5236)
                     /locus_tag="WGQ68_001550"
     CDS             complement(4298..5236)
                     /locus_tag="WGQ68_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715327.1"
                     /translation="MDAYTEAEAPPQSIVELVPVLIAVTDGGLRVLTVAQGTLLPNGP
                     LSPLRNSLQAGVKLWVAKQTSQPMGYVEQLYTFVDTHRRNEHGMPVLYVSYLGLVREA
                     ADSILHPDAKWQDCYGYFPWEDLRTDGGQRDAVVSRLRIWANSADTEEVRQMRLKRIH
                     LCWGVEPENWSEEYVLQRYEMLYESGLIVEAAEPQANFDFALTGQPMRHDHRRVLATA
                     LSRLRAKIKYRPVIFELMPPEFTLLQLQNSVEAISGRLLHKQNFRRQIQQQNLIEPSD
                     TGVSGSKGRPAQLCRFRDDVLPDRLISDIGLPLGSR"
     gene            5543..6655
                     /gene="nadA"
                     /locus_tag="WGQ68_001551"
     CDS             5543..6655
                     /gene="nadA"
                     /locus_tag="WGQ68_001551"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEZ0715328.1"
                     /translation="MQTAARRSFDYDMPLIQTPTSACQIRQAWAKVADTPDRETADRL
                     KDEIKALLKEKNAVLVAHYYVDPLIQDLALETGGCVGDSLEMARFGAEHEAGTLVVAG
                     VRFMGESAKILCPEKTVLMPDLEAECSLDLGCPEEAFSAFCDQHPDRTVVVYANTSAA
                     VKARADWVVTSSVALEIVSYLKSRGEKLIWGPDRHLGDYIRRETGADMLLWQGSCIVH
                     NEFKGQELAALKAEHPDAVVLVHPESPQSVIELGDVVGSTSKLLKAAVSRPEKKFIVA
                     TDLGILHEMQKQAPDKEFIAAPTAGNGGSCKSCAFCPWMAMNSLGGIKYALTSGRNEI
                     LLDRKLGEAAKLPLQRMLDFAAGLKRGDVFNGMGPA"
     gene            6707..8215
                     /gene="nadB"
                     /locus_tag="WGQ68_001552"
     CDS             6707..8215
                     /gene="nadB"
                     /locus_tag="WGQ68_001552"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEZ0715329.1"
                     /translation="MQTDCDVLIAGNGLAALTLALSLPESFRIVILCKNRLDDTASRH
                     AQGGIAAAWSGEDDIEKHVADTLEASAGLCDEAAVRTILSQGKPAIEWLLAQGVAFDR
                     NYDGLHLTREGGHTCRRIAHVADYTGEAVMQSLLAQIRRRPHIRVCERQMALDIQTES
                     GVACGLIVLDRRTQETYLIRARHTVLAGGGLGQIYAATTTPPECTGDAIAMAIRAGCA
                     VENLEFIQFHPTGLARSSENGRTFLISEAVRGEGGILTNQAGERFMPHYDRRAELAPR
                     DIVARAIAAEIAKQTPDFVSLDISRQPEAFVRRHFPSIHRHCLSQCGLDITRQAIPVR
                     PVQHYTCGGIQTDPCGRTSLPQLYALGETACTGLHGANRLASNSLLECVVTARLAAQT
                     IADGQAFQAKTSPRPSENPSAEAGIFADDLQNTFSRPVLQTFNQRHLGILRSDTGLRR
                     AIAQLRLWKQNQAEPHTASEYENRNLLECSLAVAQAAYRRRQNIGAHFNSDC"
     gene            complement(8391..9056)
                     /gene="pgmB"
                     /locus_tag="WGQ68_001553"
     CDS             complement(8391..9056)
                     /gene="pgmB"
                     /locus_tag="WGQ68_001553"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEZ0715330.1"
                     /translation="MTFTAVLFDLDGVITDTAEYHYRAWKKLAEELGIGIDRKFNEQL
                     KGVSRDDSLKRILAHGGKTVGEAEFAELTRRKNDNYVEMIQAVKPEDVYLGILPLLEA
                     LKANGKKIALASASKNGPFLLERMGLTHFFDTVADPAAVAHSKPAPDIFLAAAEGVGA
                     DIRRCIGIEDAAAGVAAIKAAGALPIGVGKAEDLGSDIALVSDTAELTYAYLQNVWAQ
                     SGR"
     gene            complement(9069..11327)
                     /locus_tag="WGQ68_001554"
     CDS             complement(9069..11327)
                     /locus_tag="WGQ68_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEZ0715331.1"
                     /translation="MYTRIMEISPWTLRSAKLEKEHKRLQESLTSLGNGYMGMRGSFE
                     ETYSADSHLGTYIAGVWFPDKTRVGWWKNGYPKYFGKAINALNFSKVKIFVDGQEVDL
                     AKNDVAGFSVELDMQHGVLRRSFTVFGVRFNVCKFLSVAQKELAVIRWEAVSVDGKTH
                     QVRIDSIIDADVKNEDSNYEEKFWQVLDKGVSDSLSYIAAQTVANPFGVEQFIVNAEQ
                     TFAGSFKALGGRQTDWQVSNSFEAEVGGTPETFEKRVIVTTSRDYQGLEAVKAAGRAL
                     SEKVAGVAFETLLDAHKAGWLHRWEIADVVIEGNDEAQQGIRFNLFQLFSTYYGEDAR
                     LNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAEPEVTRNLLQYRRNQLPQAQHNARE
                     QGLAGALYPMVTFTGIECHNEWEITFEEIHRNGAIPYAIYNYTNYTGDESYLAKEGLE
                     VLVEVSRFWADRVHFSKRSGKYMIHGVTGPNEYENNINNNWYTNTLAAWVLDYTREAL
                     AKYPRPDLNVSAAELEKWADISANMYRPHDEELGVFVQHDGFLDKDIRPVSALSPDDL
                     PLNQKWSWDKILRSPFIKQADVLQGIYFFGDRFNIDEKRRNFDFYEPMTVHESSLSPC
                     IHSILAAELGKEEKAVEMYRRTARLDLDNYNNDTEDGLHITSMTGSWLAIVQGFAQMK
                     TWGGKLSFAPFLPSAWTGYTFHINYRGRLIKVAVGKENVVFTLLKGEPLDLQVYGKDI
                     TLNGSHTAALEK"
BASE COUNT         2469 a         3055 c         3147 g         2699 t
ORIGIN      
        1 atccatccat acggaaacct gcaccacgtc attcctacga acctacattc cgtcattccc
       61 acgaaagtgg gaatccagaa cgtaaaatct gaagaaaccg ttttacccga taagtttccg
      121 tgccgaccgg tctggattcc cacctgcgcg ggaatgacgg ttcgggtatt tctgacgatt
      181 catcgagccg tagcaactgt atttttcacc ccgtcgggca aaaataccaa aactcaaatc
      241 aagccgtttg gataccgttt tcggcggtat cgttttcggc aaaataatca cgcatccggg
      301 tattcgatat cgtcagcagt ttgcgcatac atgccgtaac ggcaacctta tacggcttac
      361 ccttggacag caggcgttgg tagaaatccc gaataagcgg ttcaaaacgt gtcgctgcca
      421 cggtagccat atacagtgcc ttacgcaccg cagaccttcc gccaaagcag cggcttttga
      481 atttggtttc ctcgctctcc ctcgggtgcg gggcaatgcc ggccaaactc gctatccgtt
      541 tgtgcgacag ccgccccaat tcgggcagca tcgccatcag cgtagccgtc gttatcgaac
      601 cgatgccttt gatttgctcc gccacttggg ctttgccgtc aaaatgcgtg tgggtgtggt
      661 cgtcgatttg tttgtccaat tcgtcaatca gccggtcaaa atgggcaatc agttgtttga
      721 cgcttccgac ttgcgtttca tgaaactgat gcagatatag tggattaaca aaaatcagga
      781 caaggcgacg aagccgcaga cagtacaaat agtacggcaa cgctgtactg gtttaaattt
      841 aatccactat attttgtaat agctgccatt atgcctacta ggatatatac cttctatata
      901 atccttattt aaataaaaac ccttataaaa atctgtgtta tctgtcggga ttgaaaaaaa
      961 gattttatct ttctcatcag gtttatattt gctatcaata tcaaaaagag aataaggatt
     1021 acaatgagtt cgacatacta ggtaaaaatt tatatcttgg atcatgaaat attcgatatt
     1081 tatcaggatc gaaagatgct aaaaattcat caatatcggt actggatatt ttgtcatcac
     1141 taataaacat cgctagagat gggcgacttt gattttccat ttgttgatta gtaaaggata
     1201 taaatactaa cttgccattg tcaaaccaca tagatttata tagtggatta aatttaaacc
     1261 agtacgtcgt tgcctcgcct tgccgtacta tttgtactgt ctgcggcttc gtcgccttgt
     1321 cctgattttt gttaatccac tatagttatt gcctttggga gaatcaattc tgacagcaca
     1381 taacataggt tcatctttac agtcgttata ttgggtttca tcaaactcaa ctgcatagca
     1441 caataaaggc aataggaaaa aaaggagatt aatttattac tatacataag gcagcctgat
     1501 tattcattct aaaataaatc cggtttttaa tatttgattc tgctccggat tatatacata
     1561 ataaatttta ctgctccaaa atctgatatt aaccctatgg atttcataaa tttttcagaa
     1621 aaataggctg tgcttgaaca atccgccgac tccggctgtt tgtcagggaa aaggcaatta
     1681 ttttcggtta gtcagcagcc tatcctattg aatcacttaa agatatggat gtttgtcaac
     1741 gccatatcaa aacacggtct tttaccaatc gaattctgcc gtcaccggcg cgtggtcgct
     1801 cggacgctcc agcgcgcgcg tctccaaatc gacgcggaca tccttcaacg ccgccgccat
     1861 cgcaggcgac accaaaatat ggtcgatacg caggcccagt ttgcgttgga acatcgcgcc
     1921 gcgatagtcg aaccatgtgt aaaacgcgcc ttcgggatgg acttggcgca ggctgtcggt
     1981 cagtcccaaa tccagcaggt tttgaaacca ctgccgttcg acggacgaac agtggatttt
     2041 ttcgtgccat ttttcagggt cgtaacagtc cgcatcggca ggcgcgatat tgaaatcgcc
     2101 cagtagcacc agtttgccgt agcgggtcat ttcatcgcgg acaaactccg tcagtgcggc
     2161 aaaccattgt tctttatatt tgaatttcgg gctgtcaagg gcttcgccgt tgacgcaata
     2221 gacattgatg acgcgcacac cgccgacggt tgccgcaatc acgcgccgtt gcggatcgtc
     2281 cggcagcgcg ggcagcccga tatgcacgtc ttccggcgcg ttgcggctga cgattgccac
     2341 gccgttgtag gttttctgcc cgctccaaac acagtgccag cccatcattt gcaaagcggc
     2401 ggccggaaat ttgtcctgat cgagtttgag ttcctgcaaa accaaaatat cgggcggatt
     2461 gtcggcaagc aggttttgca cctgcggcag ccgcacattg agcgaattga cgttccaagt
     2521 ggtgattttc ataacatttc cgaaaaatgc cgtctgaaac gttcagacgg caaaaggaaa
     2581 ggattttaac accataccgc gcgtttgttt tccaaatcgc ggattgtccg cagaatcgtt
     2641 gccgcgtctt cggaaatcag gcggtattcg ataatgcggt ggtaacgcgt aaagtccacc
     2701 agaccgcccg ccctcaggcg gttcaaatgg ccggaaacgg cggtggccgg cagggataag
     2761 gattttgcca gttcggcgat attgcgttcg ctgtccaaca gttggatcag tatcgccata
     2821 cgttcgggat gcgccataag tttgagtatg gtgtggagcg gtattgtgtt catcggtatt
     2881 cctaaaacct ttgccgcccg ccaagcggat tgacgggcga cagatatgga tgattgtgat
     2941 tatctttata aatcgtatta taaatcaaat atttatggat gattgtacag gattgtatgt
     3001 tccttttccc actttttttg caaacacgcg taaaggcggg ctttcagacg gcataaaact
     3061 gccgccccta ccctgcctca ccctgttgcc gaacggaatc atccgcattg ttcaaaagat
     3121 gccttacaac cgccgcacgt cttgccctgc cgatgcctcc gtgccttcgg gcaaacaaaa
     3181 tacctgcctg aaacgctgtt ttttctaaag catcacgacc ttcacgaaaa atgccgtctg
     3241 aagaatattt cagacggcat ttcctccttg aggcaataac cggattacgg ttaggcggtt
     3301 ccttgtctga catatcagac aactgccgac accctaaaac tcatgccaag aaatccaaac
     3361 ctatgtccaa cgagcggctg ctgtgggtca gatagccgag ggcgatgccg tccacgccgg
     3421 tttgcgccac gcgcttcagg cggtcgaagc cgatgccgcc cgatgcttcg caatagacgg
     3481 tgtgggggtg ggcggtttgc gtgtggcagc ggtttgccgc ttctttcagg gtttcgtcgt
     3541 ccatgttgtc cagcaaaatc cgttccgcgc ccgctgcgat ggcttcgtcc agttgtgcca
     3601 acgtgtccac ttcgatttcc acgcaggtca atgctccgac cgcctgtttt gcctgccgaa
     3661 cggcttgggc gatgctgccg caataggcga ggtggttgtc tttgatgagt acggcatcgt
     3721 ccaaacccat gcggtggttc acgccgccgc ccgccctgac ggcgtatttt tgcaggacac
     3781 gcagcagggg gagggttttg cggctgcaca cgatgtctgt accgtattcg gcaacttcgg
     3841 caacggcacg cgcggtggcg gtggcgatgc cgcttaagtg cgtgaggtag ttgagcgcgg
     3901 tgcgttcggc ggcgagcagc gcgcgggcgt tgccttcgac ggcggccagc gtctgacctg
     3961 cgcggacgat ttgcccgtct cggatttcgg cttggaagcg gacggacgga tccatcgtct
     4021 gaaaggcgag gcgcgccaag tccatgccgg cgataacgcc gtcttcgcgg ctgacgagga
     4081 agagtttggc ggttttatcg ggcgcgatga cggcggcgga cgtaatatcg ccgcgcctgc
     4141 ccaaatcttc gctcaaggct tgttccacca tggggcgcaa tagggtgtcg ggcaggggaa
     4201 agagggtgtt ttcagacggc atggggtttc ctttgttgaa aaagatgggt gttgcaccga
     4261 tggggcaaaa cttcatttga tgccgtctga aaacgggcta acggctgccc agcggcaatc
     4321 cgatgtccga aatcagcctg tcgggcagga cgtcgtcgcg gaagcggcaa agctgcgcgg
     4381 gacggccttt gctgcccgat acgccggtat ccgacggctc gataaggttt tgctgctgaa
     4441 tctggcggcg gaagttttgc ttgtgcagca atcttccgct gatggcttcg acactgtttt
     4501 gcagttgcag cagcgtgaat tcgggcggca tcagttcaaa aatcacgggg cggtatttga
     4561 ttttggcgcg caggcgggac agggcggtcg ccagtacgcg acggtgatcg tggcgcatgg
     4621 gctgccccgt gagcgcgaag tcgaagtttg cctgcggctc ggcggcttcc actatcagac
     4681 cgctttcata cagcatttca tagcgttgta aaacgtattc ttccgaccag ttttccggtt
     4741 cgacccccca gcacaaatga atgcgcttga gccgcatttg acgcacttcc tccgtgtccg
     4801 ccgaatttgc ccaaatgcgc aggcggctga cgacggcgtc gcgctgcccg ccgtcggtgc
     4861 gcaagtcttc ccacgggaaa tagccgtagc agtcctgcca tttcgcatcc gggtgcagga
     4921 tgctgtcggc tgcctcgcgc accagcccca aatagctgac gtacaagacg ggcatgccgt
     4981 gttcgttgcg gcggtgggta tcgacaaagg tgtaaagctg ttccacatag cccataggct
     5041 gcgaagtctg cttggcaacc cacagcttca cgcccgcctg cagggaattg cgcagagggg
     5101 agagcgggcc gttgggcagg agtgtgcctt gggcgacggt taatacccgc aggccgccgt
     5161 cggtaacggc aatcaatacg ggaaccagct cgacgatgct ttgcggcggg gcttcggctt
     5221 cggtgtaggc atccatgctc ggctttctgc gggtgggaaa ggctttattg tatagtggat
     5281 taaatttaaa ccagtacagc gttgcctcgc cttgccgtac tatttgtact gtctgcggct
     5341 tcgtcgcctt gtcctgattt ttgttaatcc actataaacc aaagcggttt gtcggaacga
     5401 agcgggtcgg ttttacggta tcgcaaacct gttttcagac gaccttgctt attatactca
     5461 atttgagcat aatataaaaa tatttcaaaa tataaacggc aaatctttat gccgtctgaa
     5521 aaaccagcca taaggaagtc tcatgcaaac cgccgcccgc cgctcgttcg actacgatat
     5581 gccactcatc caaacgccga cttccgcctg ccaaatccgt caggcgtggg cgaaggttgc
     5641 cgacacgccc gaccgcgaga cggcagatcg tctgaaagac gaaatcaagg ctttgctgaa
     5701 ggagaaaaac gcggtcttgg tggcgcatta ttatgttgat ccgctgattc aggatttggc
     5761 tttggaaacg ggcggatgcg tgggcgattc tctggaaatg gcacgcttcg gcgcggaaca
     5821 cgaagccggt acgctggtgg tggcgggcgt gcgtttcatg ggagagagcg cgaaaatcct
     5881 ctgccctgaa aaaacggtgc tgatgcctga tttggaagcg gaatgttctt tggatttggg
     5941 ttgcccggaa gaggcgtttt cggcgttttg cgaccaacac cccgaccgca cggtggtggt
     6001 gtacgccaac acttccgccg ccgtgaaagc gcgtgccgat tgggtggtaa cgtcttcggt
     6061 ggcgttggaa atcgtgtcgt atctgaaatc gcgcggcgag aagctgattt gggggcccga
     6121 ccgccacctc ggcgactaca tccgccgcga aacgggcgcg gatatgctgt tgtggcaggg
     6181 ttcgtgcatc gtccacaacg aatttaaagg gcaggagctg gcggcgttga aggcggaaca
     6241 ccccgatgcg gtggtgctgg ttcatcccga atcgccacaa agcgtcatcg aactgggcga
     6301 cgtggtcggc tcgaccagca aactgctgaa agctgccgta tcacgccccg aaaaaaaatt
     6361 catcgtggcg accgatttgg gcatcctgca cgaaatgcaa aagcaggcgc ccgacaaaga
     6421 atttatcgcc gcgccgacgg cgggcaacgg cggaagttgc aaaagctgcg cgttctgccc
     6481 gtggatggcg atgaattcgc tgggcggcat caaatacgcc ctgacaagcg gacgtaacga
     6541 aatcctgttg gacaggaagc tgggcgaagc cgccaaactg cctttgcagc gtatgctcga
     6601 cttcgcggca ggactcaaac gcggggatgt gttcaacggc atggggcccg cctgatttgc
     6661 cgtccatatt gccgtttcag acgacctctc caacaaggac aacaccatgc aaaccgattg
     6721 cgacgtattg attgccggaa acgggctggc ggcactgacg ctcgccctgt cgctgcctga
     6781 atcgttccgc atcgtcattt tgtgcaaaaa ccggctggac gacaccgcca gccgtcatgc
     6841 gcaaggcggg attgcggcgg cgtggtcggg agaggacgac atcgaaaaac acgtcgccga
     6901 tactttggaa gcgagcgcag gtttgtgcga cgaagccgcc gtccgcacca tcctgtcgca
     6961 gggcaaaccg gcaatcgaat ggctgctggc gcagggcgtg gcgttcgacc gaaattatga
     7021 cggcctgcac ctgacgcgcg aaggcgggca cacctgccgc cgaatcgccc acgtcgccga
     7081 ctacacgggc gaagccgtca tgcagagcct gcttgcccaa atacgccgcc gcccgcacat
     7141 ccgcgtttgc gagcggcaga tggcgttgga cattcaaacc gaatcaggtg tggcgtgcgg
     7201 actgatcgtc cttgaccgcc gaacgcaaga aacctacctc atccgcgccc gccataccgt
     7261 actcgcaggc ggcggcttgg gacagattta cgccgccacc accacgccgc ccgaatgcac
     7321 gggcgacgcc atcgccatgg cgatacgcgc aggctgtgca gttgagaacc tcgaatttat
     7381 ccaattccac cccacaggct tggcaagatc gtctgaaaac ggccgtacct tcctgatttc
     7441 cgaagccgtg cgcggcgaag gcggcatcct gaccaaccaa gcgggcgaac ggtttatgcc
     7501 gcattacgac cgccgcgccg aactcgcgcc gcgcgacatc gttgcccgcg ccatcgccgc
     7561 cgaaatcgcc aaacaaactc cagacttcgt ctcgctcgac atcagccgcc aacccgaagc
     7621 gttcgtccgc cggcattttc cgtccatcca tcggcactgt ttatcccaat gcggcctgga
     7681 catcacgcgc caagccatcc ccgtccgccc cgtgcaacac tacacctgcg gcggcatcca
     7741 aaccgacccc tgcggcagaa cctccctgcc gcagctctac gccttaggcg aaaccgcctg
     7801 caccggcttg cacggagcca accgcctcgc cagcaactcc ctgctcgaat gtgtcgttac
     7861 cgccagactt gccgcccaaa ccatcgcgga cggacaagca ttccaagcca aaacgtctcc
     7921 aaggccgtct gaaaacccct ccgccgaagc aggcatcttt gcagacgacc ttcaaaacac
     7981 attcagccgc cccgtcctgc aaacgttcaa ccaacgccat ctcggcatcc tacgcagcga
     8041 taccggcctg cgccgcgcca tcgcccaact gcggctttgg aagcaaaacc aagccgaacc
     8101 gcacaccgcg tccgaatacg aaaaccgcaa cctgctcgaa tgcagcctcg ccgtcgccca
     8161 agccgcatac aggcggagac agaacatcgg cgcgcatttt aatagtgatt gttaaggttg
     8221 gctggttaga aaaaatcgtt caaatgaatt ttaatagcat ggttaagaga ttggcattca
     8281 gtttttaggg ttgcgtaagt tgggttatca atctgttcta aaagcagaaa ggccgtctga
     8341 aacactgttc agacggcctt ttacttcctt gacactttat ctgacgcgtt ttacctgccc
     8401 gactgtgccc acacgttttg caggtaggcg taggtcagct cggcggtatc ggacaccagc
     8461 gcgatgtcgc tgcccaagtc ttcggctttg cccacgccga taggcaaggc gccggcggct
     8521 ttgatggcgg cgacacctgc ggcagcgtct tcaatgccga tgcagcggcg gatgtccgcg
     8581 cccacgccct cggcggcggc gaggaagatg tcgggggcgg gtttggaatg cgcgacggcg
     8641 gcagggtcgg cgacggtgtc gaagaagtgg gtcagcccca tgcgttcgag caggaagggg
     8701 ccgtttttac tggcggacgc aagggcgatt tttttgccgt ttgcttttaa tgcttccagc
     8761 agcggcaaaa tgccgagata cacgtcttcg ggtttgactg cctgaatcat ctcgacgtag
     8821 ttgtcgtttt tgcggcgggt cagttcggcg aactcggctt cgccgacggt tttgccgccg
     8881 tgtgcgagga tgcgtttgag cgaatcgtcg cgcgacacgc ctttgagctg ctcgttaaac
     8941 ttgcggtcta tgccgatgcc cagttcttcg gcgagctttt tccatgcgcg gtagtggtat
     9001 tcggcggtgt cggtgatgac gccgtcgaga tcgaagagca ctgcggtaaa cgtcattttg
     9061 cgccctcctt atttttccaa cgcggcggta tggctgccgt tgagtgtgat gtctttgccg
     9121 tacacctgca aatcgagcgg ctcgcctttg agcagggtga agacgacgtt ttctttgccg
     9181 acggcgactt taatcagacg gcctcggtag ttgatatgga aggtgtagcc tgtccacgcg
     9241 ctcggcagga acggtgcgaa gctgagttta ccgccccaag ttttcatttg ggcgaaacct
     9301 tggacgatgg cgagccacga gccggtcata gaagtaatgt gcaggccgtc ttcggtgtcg
     9361 ttgttgtagt tgtccaagtc caggcgggct gtgcgccggt acatttccac ggctttttct
     9421 tctttgccca gttcggcggc gagaatagag tggatacacg gcgacaacga gctttcgtgc
     9481 acggtcatcg gttcgtagaa gtcgaaattg cggcgttttt cgtcgatatt gaaacggtcg
     9541 ccgaagaaat agatgccttg caagacatcc gcctgtttga tgaagggcga acgcaggatt
     9601 ttgtcccacg accatttctg gttgagcggc aaatcgtcgg gcgaaagcgc ggacacgggg
     9661 cggatgtctt tgtcgaggaa tccgtcgtgt tgtacgaata cgccgagttc ttcgtcatga
     9721 ggacggtaca tattcgcgct gatgtccgcc catttttcca actcggcggc gctcacgttc
     9781 aaatccggac gcgggtattt cgccagggct tcgcgggtgt agtccaatac ccatgcggcg
     9841 agggtgttgg tgtaccagtt gttgttgatg ttgttttcgt attcgttcgg gccggttacg
     9901 ccgtgaatca tgtatttgcc gctgcgtttg gagaagtgga cgcggtccgc ccagaagcgg
     9961 gacacttcga ccaagacttc caagccttct ttggcaagat agctttcgtc gccggtgtag
    10021 ttggtgtagt tgtagatggc gtaaggaatc gcgccattgc ggtggatttc ctcgaaggtg
    10081 atttcccatt cgttgtggca ctcgatgccg gtaaacgtta ccatcggata gagtgcgccc
    10141 gccaagccct gttcgcgcgc attgtgctgc gcctgcggca gttggttgcg gcggtattgc
    10201 agcaggttgc gggtaacttc gggttccgcc agcgcgaggt agagcggtac ggcgtaggct
    10261 tcagtatccc aataggttgc gccgccgtat ttttctccgg taaagccttt cgggccgatg
    10321 ttcaggcgcg cgtcttcgcc gtagtaggtg gagaacagtt ggaacagatt gaaacggatg
    10381 ccttgctgcg cttcgtcgtt gccttcgatg accacgtcgg cgatttccca acggtgcagc
    10441 cagcctgctt tgtgcgcgtc cagcaaggtt tcaaacgcaa cgcctgcgac tttttccgac
    10501 aaagcgcggc ctgcggcttt cactgcttcc aagccctgat aatcgcggct ggtggtaaca
    10561 ataacccgtt tttcaaaggt ttcgggtgtg ccgccgactt cggcttcaaa agaattggag
    10621 acctgccagt cggtttgtct gccgccgagg gctttaaagc tgccggcgaa ggtttgctcg
    10681 gcgttgacga taaattgttc cacgccgaaa gggttggcaa cggtttgggc ggcaatgtag
    10741 gagagactgt ctgaaacgcc tttgtccaat acctgccaga atttttcttc gtagttggag
    10801 tcttcgtttt tcacgtcggc atcgatgatg gaatcgatgc ggacttggtg ggttttgccg
    10861 tcaacggata cggcttccca gcggataacc gccagctctt tttgcgcgac ggacaggaat
    10921 ttgcacacat tgaaacgcac accgaatacg gtgaacgagc ggcgcaacac gccgtgctgc
    10981 atatcaagtt cgacggagaa gccggcaaca tcgtttttcg ccaagtccac ttcctgcccg
    11041 tcgacaaaga ttttgacttt gctgaaattg agcgcgttga tggctttacc gaaatatttg
    11101 gggtagccgt ttttccacca gccgacgcgg gttttgtcgg gaaaccacac accggcgatg
    11161 taggtaccca agtggctgtc ggcggaatag gtttcctcaa agctgccgcg catacccata
    11221 tagccgttgc ccaaactggt caggctctct tgcagccgtt tgtgttcttt ttccagcttt
    11281 gccgaacgca gcgtccaagg gctgatttcc ataattcttg tgtacattta tgaagctcct
    11341 gtttggattg atttgaggga atggtgaaat
//
LOCUS       DAWFHY010000068        11160 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.068, whole genome shotgun sequence.
ACCESSION   DAWFHY010000068 DAWFHY010000000
VERSION     DAWFHY010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 11160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11160
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.068"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..167)
                     /locus_tag="WGQ68_001555"
     CDS             complement(<1..167)
                     /locus_tag="WGQ68_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0715332.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRTR
                     YLRDHRGRPAYP"
     gene            354..536
                     /locus_tag="WGQ68_001556"
     CDS             354..536
                     /locus_tag="WGQ68_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715333.1"
                     /translation="MFWYVIGFCSFVVALLSLWVNAGAFGMQEDDTPQSDYEKRLGLG
                     AKLKNKNTPDTEEKQQ"
     gene            complement(598..1599)
                     /locus_tag="WGQ68_001557"
     CDS             complement(598..1599)
                     /locus_tag="WGQ68_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEZ0715334.1"
                     /translation="MIDGQTTEPKQKTRIILAPMQGLVDDVMRDLLTRIGGYDECVSE
                     FVRITHTVHSRSIWLKYVPEIANGNKTFSGTPCTVQLLGSDADNMAANALEAVRFGAN
                     KIDLNFGCPAPTVNKHKGGAILLKEPELIFHIVKTLRGRLPAHIPLTAKMRLGYEDKS
                     RALECACAIAEGGACGLTVHARTKAEGYEPPAHWEWIRKIRDSVNIPVTANGDVFSLQ
                     DYIGIKTISGCNSVMLGRGAVIRPDLARQIKQYENGGPVKDTDFAEVSKWIRQFFELC
                     LTKEANNKYPIARLKQWLGMMKKTFDPAQTLFDRVRTVKDADEVRRILNAFEHEINV"
     gene            complement(1676..5509)
                     /locus_tag="WGQ68_001558"
     CDS             complement(1676..5509)
                     /locus_tag="WGQ68_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HEZ0715335.1"
                     /translation="MTTTTAPQRIREIPYNYTSYTDREIVIRLLGDEAWQILQDLRGQ
                     RKTGRSARMLFEVLGDIWVVVRNPYLVDDLLEHPKRRAALVREMRHRLNEIRKRRDDN
                     RQVDVLVAAAEKAVERFDSSFDETSQKRRQILERLSKITKPHNIMFDGLARVTHVTDA
                     TDWRVEYPFVVVNPDTEAEIAPLVRALIELDLVIIPRGGGTGYTGGAIPLDANSAVIN
                     TEKLDKHRGVEYVELAGLDGKHPIIRCGAGVVTRRVEETAHQAGLVFAVDPTSADASC
                     VGGNVAMNAGGKKAVLWGTALDNLAYWNMVNPQGEWLRIERVRHNFGKIHDEETAVFD
                     VHTLDSDGINIVKTERLEIPGHKFRKVGLGKDVTDKFLSGLPGVQKEGTDGIITSVAF
                     VLHKMPKYTRTVCMEFFGTVATATPSIVEIRDFLLAHDSVRLAGLEHLDWRYVRAVGY
                     ATKAAGKGRPKMVLLADVVSDDEAAVEAAAEHICELARARDGEGFIAVSPEARKTFWL
                     DRSRTAAIAKHTNAFKINEDVVIPLERLGEYSDGIERINIELSIQNKLKLCAALEQYL
                     SGKLPIDKMGTDLPTAELLGERGKHALAHVSAVKARWEWLLANLDAPLADYKARYGAA
                     VHAAPEAKDNESCFIAFRDFRLRVSVKADVMKPLSEIFSGKTDTKIIQGLGKIHAKTV
                     RSRVFVALHMHAGDGNVHTNIPVNSDDAEMLQTAYRSVERIMKIARSLNGVISGEHGI
                     GITKLEFLSDEEMQPFWDYKNQVDPKHTFNRHKLMKGSDLRNAYTPSFELLGAESLIM
                     EKSDLGTIADSVKDCLRCGKCKPVCSTHVPRANLLYSPRNKILGVGLLTEAFLYEEQT
                     RRGVSVKHFEELMDIGDHCTVCHRCVKPCPVNIDFGDVTVAVRNYLADSGHKRFAPAA
                     SMGMAFLNATGPKTIKALRAAMIQTGFPAQNFAYKIGKLLPIGTKKQKAEPKATVGTA
                     SIKEQVIHFINRPLPKSVPAKTPRSLLGIEDGKSIPIIRNPAAPEDAEAVFYFPGCGS
                     ERLFSQIGLAVQAMLWHVGVQTVLPPGYMCCGYPQDAGGNKAKAEEMSTNNRVAFHRM
                     ANTLNYLDIKTVVVSCGTCYDQLEKYRFEEIFPGCRIIDIHEYLLEKGVKLDGVKGQQ
                     YLYHDPCHTPIKTMNATQMASSLMGQKVVLSDRCCGESGMFAVKRPDIATQVKFRKQE
                     EIEKNLKELPQGEPVKMLTSCPACLQGLSRYADDNNMPADYIVIEMAKHILGENWLDE
                     FVKKANNGGVEKVLL"
     gene            5770..5866
                     /gene="ffs"
                     /locus_tag="WGQ68_001559"
     ncRNA           5770..5866
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="WGQ68_001559"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            6034..7725
                     /gene="dld"
                     /locus_tag="WGQ68_001560"
     CDS             6034..7725
                     /gene="dld"
                     /locus_tag="WGQ68_001560"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HEZ0715336.1"
                     /translation="MSASQLLSRLTQTVGEKYIITDPAKTEQYRQGYRFGEGKALAVV
                     RPGSILEMWKILQACVEADVIVITQAANTGLTGGSTPDGNDYDRDIVIVNTMRMNIIQ
                     TINNNEQVVCLPGSTLNQLELLLKPLGREPHSVIGSSCIGASVLGGVCNNSGGALVQR
                     GPAYTEMALFAQINEEGRLELVNHLGIDLGDTPEEILTNLQGHHYQNKDITQDAGKGH
                     DHAYCEHVRQVDEPTAARFNADPARHYEASGCAGKLMVFAVRLDTFPQEKQTAVFYIG
                     TNDINELTDIRRAALGEFESLPVSGEYIHRHAFDIADVYGKDTFYVIKKFGTHQLPKL
                     FDLKARVDRFGKKASFLPKHFSDKAMQFVSKYLPDHLPKSMRDYRDKYEHHLILKMGG
                     KGVDEARAFLKEYFAHHSGAFFECNAEETQAAMLHRFAVASAAIRYRAVHDDEVEDLV
                     ALDIALRRDDRDWFEKLPSEIDNKIIHKLYYGHFMCHVFHQDYIIKKGNDCMALEHEM
                     LHLLDQRGAQYPAEHNVGHLYEAKPALKQFYRKLDPTNSFNPGIGKTSKKKNWAE"
     gene            7857..7964
                     /locus_tag="WGQ68_001561"
                     /pseudo
     CDS             7857..7964
                     /locus_tag="WGQ68_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            8020..8391
                     /locus_tag="WGQ68_001562"
     CDS             8020..8391
                     /locus_tag="WGQ68_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HEZ0715337.1"
                     /translation="MKPYKIYTHPALPPQAVKQGWSWPGLLFGTLWACFKRMWGLGLG
                     LTGAIFVLAVFAQLVYGDTPATDSAFNVLGLAVSVWFGAKGNSLYARHLLSRGYTELP
                     ETVQAANPQAALAQYFGRGGR"
     gene            8546..9094
                     /locus_tag="WGQ68_001563"
     CDS             8546..9094
                     /locus_tag="WGQ68_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0715338.1"
                     /translation="MARLTVHTLETAPEAAKARVEAVLQNNGFIPNLIGVLSNAPEAL
                     AFYQEVGKLNAANSLTAGEVEVIQIIAARTNQCGFCVAGHTKLATLKKLLSEQSVKAA
                     RALAAGEFDDAKLGALAAFTQAVMAKKGAVSDEELKAFFDAGYNQQQAVEVVMGVALA
                     TLCNYVNNLGQTEINPELQAYA"
     gene            9228..10319
                     /locus_tag="WGQ68_001564"
     CDS             9228..10319
                     /locus_tag="WGQ68_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HEZ0715339.1"
                     /translation="MNAQTLIANVAELVKTKLKPLVDDIDRKGYYPEAFMRELGAIGG
                     FGAVGTEAEGGNGLGLATQIAVLREIGKECGATSFSAWCQAACAWYLHQTPNRAVKDK
                     YLADILQGKVLAGTGMSNTVKHLADIEKHNLQAERVEGGYTVNGALPWVSNIGEDHIW
                     ANTAQIGGGYVMFITGGQWEGVSLQNCPEFCALEGTRTFSLNFKDVFIPDEDIIAAPE
                     QFADYIQSIKAGFILLQIGIGAGVIDGSLGIIRLANVVNAEVNNYLNDGYDSLKTRLD
                     GAWAETERLAGLAWSGTPDNLATLKLREAAAVLALAAAQSAALHSGAKGYLMRSPAQR
                     RVREAMFVAIVTPAIKHLRKEIAAIEAAK"
     gene            10925..11095
                     /locus_tag="WGQ68_001565"
     CDS             10925..11095
                     /locus_tag="WGQ68_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003744701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
                     /protein_id="HEZ0715340.1"
                     /translation="MAQYMCGPCGWIYDEELGDPEHGIAPGTKFEDIPDDWKCPECGV
                     DKEDFYLLDFVI"
BASE COUNT         2623 a         3057 c         3009 g         2471 t
ORIGIN      
        1 gggtaggcgg gacggccgcg gtggtctcgg aggtaacggg ttctttgacg attcaggtac
       61 tgttcgatcg gctgccaatc aatcacctgg tccaacttca atagtgggaa ccggtcgatg
      121 tgtttagcaa tcatggcttg ggcggtttgc tggaagaagg tgctcatgag aaatccccta
      181 aatgtcttgg tgggaattta ggggattttg gggaattttg caaaggtctc tatttgtact
      241 gtcttcggct cgccgccttg tccttattta aatttaattc actatatttg cccgtgcgtt
      301 tgtgataaat tcggcactgt atttgatatg attttaacaa atcgggaggg gcaatgtttt
      361 ggtatgtaat cggcttttgc tcctttgtcg tcgcgctgct gtcgctgtgg gtcaatgccg
      421 gcgcattcgg tatgcaggaa gacgataccc cgcaatcgga ttatgaaaaa cgtttgggct
      481 tgggtgcgaa gctgaaaaac aaaaatacgc cggatacgga agaaaagcag caataaagat
      541 atgaaatgcc gtctgaaaga ttttcagacg gcatttgctt tgaagcatgg gagacagtca
      601 aacatttatc tcatgctcaa atgcgttcag gattcggcgg acttcatccg catccttaac
      661 cgttcggacg cggtcaaaca gcgtttgcgc cgggtcgaac gtttttttca tcatgcccaa
      721 ccattgctta agacgggcga tcgggtattt attgtttgcc tcttttgtca ggcacagctc
      781 gaaaaactgc cgtatccatt tggaaacttc ggcaaaatcc gtgtctttga ccggcccgcc
      841 gttctcgtat tgcttgattt gccgcgccaa atcggggcgg atgaccgcgc cgcgaccgag
      901 catcacgctg ttgcagccgc tgattgtttt gatgccgata tagtcttgca ggctgaaaac
      961 gtcgccgttg gcggtaacgg gaatattgac gctgtctcgg attttcctta tccattccca
     1021 atgcgccggc ggttcgtaac cctcggcttt ggtacgcgcg tgtacggtca gtccgcatgc
     1081 gcccccttcg gcaatcgcac aggcgcattc caaagcccgg cttttgtctt cgtaaccgag
     1141 ccgcattttt gcggtgagcg gaatatgtgc gggcaaacgt ccgcgcagcg ttttgacgat
     1201 gtggaatatc agttccggct cttttaaaag gattgcgccg cctttgtgtt tgttgacggt
     1261 gggcgcgggg cagccgaagt tcaaatcgat tttgttcgca ccgaagcgga cggcttccag
     1321 cgcattcgcc gccatattgt ccgcatcgct gcccaaaagt tggacggtgc agggcgtgcc
     1381 ggaaaacgtt ttgtttccgt tggcgatttc ggggacatat tttaaccata tggatcggga
     1441 atgcacggta tgggtaatgc gtacaaattc gctgacgcat tcgtcgtagc cgccaatacg
     1501 cgtcagcagg tcgcgcatca cgtcatcgac cagaccctgc ataggggcaa ggatgattcg
     1561 ggttttttgt ttgggttcgg ttgtttgtcc gtcaatcatg atttgggtag gtattgggtt
     1621 taaacaatgc cgtctgaagg ctttcggcgt tcagacgaca tttccgtgtc cgttgttaca
     1681 gcaatacttt ttccacgccg ccgttgttgg cttttttcac aaactcgtct agccagtttt
     1741 cgccgagaat gtgtttcgcc atttcgatga cgatgtagtc ggcaggcata ttgttgtcgt
     1801 cggcgtagcg gctcaaacct tgcaggcagg cggggcagga agtcagcatt ttgacgggtt
     1861 cgccctgcgg cagctctttg aggtttttct cgatttcctc ttgtttgcgg aacttgacct
     1921 gagtggcgat gtctggccgt ttgacggcaa acataccgga ttcgccgcag cagcggtcgc
     1981 ttaaaacgac tttctgtccc atcaggctgc tggccatttg ggtggcgttc atggttttaa
     2041 tcggggtgtg gcaggggtcg tggtagaggt attgctgacc tttcacgccg tcgagtttca
     2101 cgcctttttc gagcagatat tcgtggatgt cgatgatgcg gcagccgggg aagatttctt
     2161 caaagcggta tttttcgagc tggtcgtaac aagtgccgca actgacaacg acggttttga
     2221 tgtcgaggta gttgagggtg ttcgccatac ggtggaaagc cacgcggttg ttggtgctca
     2281 tttcttcggc tttagccttg ttgccgcccg cgtcttgcgg atagccgcaa cacatatagc
     2341 cgggcggcag gacggtttgt acgccgacgt gccagagcat ggcttgaacg gcaagtccga
     2401 tttggctgaa cagacgctca gagccgcaac ccgggaagta gaacacggct tcggcatctt
     2461 cgggcgcggc ggggttgcgg atgatgggga tgcttttgcc gtcttcgatg cccaataagg
     2521 agcgcggtgt tttggcgggt acgcttttcg gcaaaggacg gttgatgaaa tggataacct
     2581 gttctttgat tgaggcagta ccgacggttg ccttgggttc ggctttttgc tttttcgtgc
     2641 cgattggaag aagtttgccg attttgtagg caaagttctg cgcggggaag ccggtctgta
     2701 tcatcgcggc gcgaagggct ttgatggttt tcgggccggt ggcgttcaaa aacgccatgc
     2761 ccatcgatgc ggcgggtgca aatcgtttgt ggccggaatc ggcaaggtag ttgcggacgg
     2821 ctacggttac gtcgccgaag tcgatgttga cggggcaggg tttgacgcag cggtggcaca
     2881 cggtgcagtg gtcgccgatg tccatgagtt cttcaaagtg tttgacggaa acgccgcggc
     2941 gggtttgttc ttcgtataag aaggcttcgg ttaacagccc tacgccgagg attttgttgc
     3001 gcggactgta cagcaggttg gcgcgcggaa cgtgggtgga gcagacgggt ttgcatttgc
     3061 cgcagcgcag gcagtctttg acggaatcgg caatcgtgcc gaggtctgat ttttccataa
     3121 tcagcgattc cgcgcccaaa agctcgaaag acggcgtgta ggcgttgcgt aagtccgagc
     3181 ctttcatcag tttgtgacgg ttgaaggtgt gtttgggatc gacttggttt ttgtagtccc
     3241 aaaacggctg catttcttcg tcgcttaaaa attcgagctt ggtaatgccg atgccgtgtt
     3301 cgccggaaat cacgccgtta agcgaacggg cgattttcat aatgcgttcg actgagcggt
     3361 atgccgtctg aagcatttcg gcatcgtctg agttaaccgg aatattggtg tgaacgttac
     3421 cgtcgccggc gtgcatatgc agggcgacaa agacgcggct gcgtacggtt ttggcgtgga
     3481 tttttcccaa gccttggata attttggtgt cggttttgcc gctgaagatt tcagaaagcg
     3541 gcttcattac gtccgctttg acagacacgc gcaggcggaa atcgcggaag gcaataaagc
     3601 aactttcatt gtctttggct tcgggcgcgg cgtggacggc tgcgccatag cgtgctttat
     3661 agtcggcaag cggcgcgtcc aagttggcaa gcagccattc ccaacgcgct ttgacggcgg
     3721 aaacgtgggc aagggcgtgt ttgccgcgtt cgcccaacag ttcggcggtc ggcaggtctg
     3781 ttcccatttt gtcgatgggg agtttgcccg aaagatattg ctccaaggcg gcacagagtt
     3841 tgagtttgtt ttggatggaa agctcgatgt tgatgcgttc gatgccgtct gaatactcgc
     3901 cgagcctttc gagtgggatg accacgtctt cgttaatttt aaaggcgttg gtgtgcttgg
     3961 cgatggcggc ggtgcggctg cggtcgagcc aaaaggtttt gcgcgcttcg ggagacacgg
     4021 cgataaagcc ttcgccatcg cgggcgcggg caagttcgca aatgtgttcg gcggctgcct
     4081 ctacggcggc ttcgtcgtcg gaaaccacgt ccgccagcaa gaccattttc ggccgtccct
     4141 tgcccgccgc tttggttgcg tagccgacgg cgcggacgta acgccagtcc aaatgttcca
     4201 aacccgccag ccgcacgctg tcgtgggcaa gcaggaaatc gcggatttcg acgatagaag
     4261 gcgtagcggt ggcgaccgta ccgaaaaact ccatacacac ggtgcgcgtg tatttcggca
     4321 ttttatgcaa cacgaaggca acgctggtga tgatgccgtc tgtaccttct ttttgcacgc
     4381 cgggcaggcc gctcaagaat ttgtcggtaa cgtctttgcc caaaccgact ttgcggaatt
     4441 tgtggccggg gatttccaag cgttcggttt taacgatatt gatgccgtct gaatccagcg
     4501 tgtgaacgtc gaacacggcg gtttcttcgt cgtggatttt gccgaaattg tggcgcacgc
     4561 gctcgatacg cagccattcg ccttgagggt taaccatgtt ccagtaggcg aggttgtcca
     4621 acgccgtccc ccacagcacg gcttttttgc cgcccgcgtt catcgccaca ttaccgccca
     4681 cgcatgacgc gtcggcagaa gtcggatcga cggcgaacac caaacctgcc tgatgcgcgg
     4741 tttcttccac ccgccgcgta accacgcccg cgccgcaccg gataatcgga tgcttgccgt
     4801 ccaagcctgc cagctcaacg tattcaacac cacgatgctt gtcgagtttt tcggtattga
     4861 tgactgcgct gtttgcgtcc aaaggaatcg cgccgccggt ataacccgtg ccgccgccgc
     4921 gcggaataat gaccaaatcc agctcgatta aggcgcgcac caaaggcgcg atttcagcct
     4981 ccgtgtcggg attgacgacg acaaacggat actccacgcg ccagtcggtt gcatcggtaa
     5041 cgtgcgttac ccgcgccagc ccgtcgaaca taatattgtg cggcttggtg attttgctca
     5101 aacgctccaa aatctgccgc cgtttttggc tggtttcatc aaaactgcta tcaaaacgct
     5161 cgactgcttt ttctgctgcg gcaaccaaca catccacttg ccgattatcg tcgcggcgtt
     5221 tgcggatttc atttaagcgg tggcgcattt cacgtaccag cgcggcgcgg cgttttgggt
     5281 gctccagcaa gtcatcgacc agatacggat tgcgcacgac cacccaaata tcacccaaca
     5341 cttcaaacag catccgcgcc gaacgcccgg ttttgcgttg accgcgcagg tcttgcagga
     5401 tttgccacgc ttcgtcgccc aataatcgga tgacgatttc gcggtcggtg taggaagtat
     5461 agttgtaggg gatttcccga atacgctgag gggcggtagt cgtggtcatg gtgtgtccgt
     5521 gtttggcagt ttttatttga atgccgtctg aaaagtcggt ttcaggcgag tttgaaaagg
     5581 cgataatgta gttcaatttt tgatactttt caatgccgaa acaggaaata cccttataga
     5641 aacaaaataa cgctttgaga aattgttgac aatattatca aatataaatt ttaggtggtg
     5701 tttccggcgt atgaccgtat caaaacggtt gagtttttca gaaaacccgt tataatgccg
     5761 tttctctgag gcgggtctcc ccgcatggca aatcggaaca ccgggtcagg ggcggaagcc
     5821 agcagcccac tccaatgcgc cagtgccggg ggtttggccc gccgccctat ttgaaacgcc
     5881 ggagcttcga tgttccggcg ttcccgtatc gcaccgatgc cgtctgaaag ccgttcagac
     5941 ggcatttcct ttactttgcc ataacgtata actacaatgt gaaaacaatt ccaatgtgca
     6001 tatgcacctg ccgacaattt taaggaaact gccatgagcg catcacaatt actgtcaaga
     6061 ctgacccaaa ccgtaggcga aaaatacatc attaccgacc ccgcgaaaac cgaacaatac
     6121 cgccaaggtt accgcttcgg cgagggtaag gcgttggcgg tggttcgccc cggaagcatt
     6181 ctggaaatgt ggaaaattct gcaggcctgc gtcgaagcgg acgtgattgt gattacgcag
     6241 gcggcgaata ccggtttgac gggtggctcg acccccgacg gcaacgatta cgaccgcgac
     6301 atcgtgattg tgaacaccat gcggatgaac atcatccaaa ccatcaacaa caacgaacaa
     6361 gtcgtctgcc tgcccggttc gaccctgaac cagcttgaat tgctgctgaa acctttgggg
     6421 cgcgaaccgc attcggtcat cggctcatcc tgtatcggtg cgtccgtctt gggcggcgtg
     6481 tgcaacaact ccggcggcgc gttggtacag cgcggcccgg cctacaccga aatggcgttg
     6541 ttcgcccaaa tcaacgaaga aggcaggttg gaactggtca accacttggg catagacttg
     6601 ggcgacacgc ccgaagaaat cctgaccaac ctgcaaggtc atcattatca aaacaaagac
     6661 atcacgcaag atgcgggcaa agggcacgac cacgcctatt gcgaacacgt ccgccaagtg
     6721 gacgaaccga ccgccgcgcg tttcaatgcc gaccccgccc gccattacga agcctcgggc
     6781 tgcgcgggca aactgatggt ttttgccgtc cgtttggaca ccttcccgca agaaaaacaa
     6841 accgccgtgt tctacatcgg cacgaacgac atcaacgagc tgaccgacat ccgccgcgcc
     6901 gccttgggcg aatttgaaag cctgcctgtt tccggcgaat acatccaccg ccacgctttc
     6961 gacattgccg acgtgtacgg caaagacacg ttttacgtca tcaaaaaatt cggtacgcac
     7021 caactgccga aattatttga cttgaaggcg cgcgtggaca ggttcggcaa aaaagccagc
     7081 ttcctgccca aacatttttc cgacaaggcc atgcagttcg tcagcaaata cctgcccgac
     7141 cacctgccca aatccatgcg cgattaccgc gacaaatacg aacaccacct gattctgaaa
     7201 atgggcggga aaggcgtgga tgaggcgcgc gcgttcttaa aagaatattt tgcacaccac
     7261 agcggcgcgt ttttcgagtg caacgccgaa gaaacccaag ccgcgatgct gcaccgtttc
     7321 gccgtcgcct ccgccgccat ccgctaccgc gccgtgcatg atgacgaagt ggaagacttg
     7381 gtcgcgctcg acatcgccct gcgccgcgac gatcgcgact ggtttgaaaa actgccgtcg
     7441 gaaatcgaca ataaaatcat ccataaactc tactatggac atttcatgtg ccacgttttc
     7501 catcaggatt acatcatcaa aaaaggcaac gactgcatgg cgttggaaca cgaaatgctg
     7561 catcttttag accaacgcgg cgcgcaatat cccgccgaac acaacgtcgg tcatttgtat
     7621 gaagccaagc ccgcgctcaa acagttttac cgcaaactcg acccgaccaa cagcttcaac
     7681 ccgggtatcg gcaaaaccag caaaaagaaa aactgggcgg aataagcgcg tccgctttga
     7741 aggcaggcaa tgccgtctga aggcaaaacg ccgttcagac ggcattgtcg ggtttgaagt
     7801 tccccgtcgg gtaatcgctt tcaaaccgta tgccgacaca cgggcgcgcc cctcccaaat
     7861 atagtggatt aaatttaaat caggacaagg caacgaagcc gcagacagta cagatagtac
     7921 ggcaaggcga ggtaacgccg tactggttta aatttaatcc actataaagt ctgatcctac
     7981 cttcccaaag ggcagggttt caaccgaaaa ggaaacacga tgaaaccata caaaatctac
     8041 acccatcccg ccctgccgcc ccaagccgtc aaacaaggct ggtcatggcc ggggctcttg
     8101 ttcggcacgc tgtgggcgtg tttcaaaaga atgtggggtt tggggctggg gctgacgggc
     8161 gcgatattcg tgctggcggt gtttgcccaa ctggtttacg gcgacacgcc cgccacagac
     8221 tcggcgttta acgtattggg cttggcggtt tccgtctggt tcggcgcaaa gggcaacagc
     8281 ctttatgcac gccacctttt atcgcgcggc tataccgaat tgcccgaaac agtccaagcc
     8341 gccaaccccc aagccgcatt ggcgcaatat ttcgggcgcg gggggcgata agcgcgtttt
     8401 cttttccatc atgccgtctg aaaaatgttc agacggcatt tcttataacc ttttgtttgc
     8461 agcacataac caatcagtct ttcctattcc gttccgaaat gcataccatg cgcccgtacc
     8521 caatatttgc gaacaaggaa agaaaatggc acgtttaacc gtacacaccc tcgaaaccgc
     8581 ccccgaagcc gccaaagcgc gcgtcgaggc ggtacttcaa aacaacggct ttatccccaa
     8641 ccttatcggc gtattatcaa acgcccccga agccttggcg ttttaccaag aagtcggcaa
     8701 gctcaacgcc gccaacagcc tgaccgccgg cgaagtcgaa gtcatccaga tcatcgccgc
     8761 ccgtaccaac caatgcggtt tttgcgtggc agggcacacc aaactcgcaa ccctgaaaaa
     8821 actcctgtcc gaacaatccg tcaaagccgc gcgcgctttg gcagcaggcg aatttgacga
     8881 tgccaaactc ggcgcgctcg ccgccttcac ccaagccgta atggcgaaaa aaggcgcggt
     8941 atccgacgag gaactcaaag catttttcga tgcgggctac aaccagcagc aggcagtcga
     9001 agtcgtgatg ggcgtagcct tggcaaccct gtgcaactac gtcaacaacc tcggacaaac
     9061 cgaaatcaac cccgaattgc aggcttacgc ctgatacggc aaaccgcccg aatatcgggc
     9121 ggtttttcaa aaacacccct caaacaaaaa caagccgccc aagcggcagg aaaagcagcc
     9181 gcgcatcagg cgcaccccga ttcccccgca aaccttaagg aacaaagatg aacgcccaaa
     9241 ccctgattgc caacgttgcc gaactcgtca aaaccaagct caaaccctta gtggacgaca
     9301 tcgaccgcaa aggatactac cccgaagcat ttatgcgcga actcggcgca atcggtggat
     9361 tcggcgcagt cggtacagaa gccgaaggcg gcaacggctt gggtttggca acgcaaatcg
     9421 ccgtattgcg cgaaatcggc aaagaatgcg gcgcaacctc tttcagtgcg tggtgtcagg
     9481 cggcttgcgc gtggtatctg caccaaacgc ccaaccgggc cgtcaaagac aaataccttg
     9541 ccgacatcct gcaaggcaaa gtattggcgg gtacgggcat gtccaatacc gtcaaacacc
     9601 ttgccgacat cgaaaaacac aacctccaag ccgaacgcgt ggaaggcggc tacacagtca
     9661 acggcgcgct gccgtgggtg tccaacatcg gcgaagacca catttgggcg aataccgccc
     9721 aaatcggcgg cggctacgtc atgttcatca caggcggaca atgggaaggc gtaagcctgc
     9781 aaaactgccc cgaattttgc gccctcgaag gtacgcgcac cttcagcctg aatttcaaag
     9841 acgtatttat ccccgacgaa gacatcatcg ccgcgcccga acagtttgcc gactacatcc
     9901 aaagcatcaa agcaggcttt atcctcctgc aaatcggcat cggcgcgggc gtaatcgacg
     9961 gcagcctcgg catcatccgc ctcgccaacg tcgtcaacgc cgaagtcaac aactacctca
    10021 acgacggcta cgacagcctc aaaacaaggc tggacggcgc gtgggcagaa accgaacggc
    10081 tcgccggctt ggcttggagc ggcacgcccg acaaccttgc taccctcaag ctgcgcgaag
    10141 ccgccgccgt actggcactt gccgccgccc aatccgccgc cctgcattcc ggcgcaaaag
    10201 gctacctgat gcgcagtccc gcccaaaggc gcgtccgcga agcaatgttt gttgccatcg
    10261 taacccccgc tatcaaacac ctacgcaaag aaatagcggc aatcgaagcc gcaaaataaa
    10321 caggcggaaa aaaccgaaac ccccaccgcg tcattcccgc gaaagcggga atccagcccc
    10381 caacgcaaca ggaatccatc ggaaaaaaca gaaaccctca aaccgtcatt cccgcaaaag
    10441 cgggaatcca gcccccaacg caacaggaat ccatcggaaa aaacagaaac cctcaaaccg
    10501 tcattcccgc aaaagcggga atccagcccc caacgtaaca ggaatccatc ggaaaaaaca
    10561 gaaaccctca aaccgtcatt cccgcaaaag cgggaatcca gccccctaac gcaacaggaa
    10621 tccatcgaaa aaaccgaaac ctccaaaccg tcattcccac ggaagtggga atccagtaat
    10681 cgaaaaacca caggaatcta tcgaaaaaaa accaacccca aaaaaaccgg gcggataccg
    10741 caccatcctc ctaaaccctg atttatagcg gatgaacaaa aatcagggca aggcggcgaa
    10801 gccgtagaca gtacaaatag tacgggtgct tcagtacctt agagaatcgt tctctttgag
    10861 ctaagacgag gcaacgccgt atcggttttt gttaacccac tatacaaaaa acaaggaaac
    10921 acaaatggcg caatatatgt gcggcccctg cggctggatt tacgatgaag aactcggcga
    10981 cccagaacac ggcatcgccc ccgggacaaa gtttgaagac atccccgacg actggaaatg
    11041 ccccgaatgc ggcgtggaca aagaagattt ctacctgttg gatttcgtga tatagcggca
    11101 gccgcaccct tagggaaaac cccgtcatcc ggcgttccgc ctgcggcggc agggaacacc
//
LOCUS       DAWFHY010000069        10892 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.069, whole genome shotgun sequence.
ACCESSION   DAWFHY010000069 DAWFHY010000000
VERSION     DAWFHY010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10892
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.069"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(801..2018)
                     /locus_tag="WGQ68_001566"
     CDS             complement(801..2018)
                     /locus_tag="WGQ68_001566"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEZ0715341.1"
                     /translation="MALIVHKYGGTSVGSPERIKNVAKRVAKARAEGHDIVVVVSAMS
                     GETNRLVALAHEMQEHPDPRELDVVLSTGEQVTIGLLAMALKDIGVDAKSYTGWQVAL
                     KTDTAHTKARIESIDDEKMRADLDAGKVVIVAGFQGISSEGDISTLGRGGSDTSAVAL
                     AAALKADECQIYTDVDGVYTTDPRVVPEARRMDTVTFEEMIELASLGSKVLQIRSVEF
                     AGKYKVRLRVLSSLQDGGNGTLITFEEDDNMERAAVTGIAFDKNQARINVRGVPDKPG
                     VAYQILGAVADANIEVDMIIQNVGSEGTTDFSFTVPRGDYKQTLEILSERQDSIGAAS
                     IDGDDTVCKVSAVGLGMRSHVGVAAKIFRTLAEEGINIQMISTSEIKVSVLIDEKYME
                     LATRVLHKAFNLG"
     gene            complement(2220..2948)
                     /gene="rph"
                     /locus_tag="WGQ68_001567"
     CDS             complement(2220..2948)
                     /gene="rph"
                     /locus_tag="WGQ68_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEZ0715342.1"
                     /translation="MPDYIRISRAADSLRDIKITPHFLPHADGSCLIECGNTKVICTA
                     SIDENVPPFLRGKNQGWVTAEYGMLPASTASRMRREASAGKQSGRTQEIQRLIGRSLR
                     AVVDMEKLGERQILIDCDVIQADGGTRTASITGAFVALQIAVGKLVSDGILSENPIRE
                     AVAAVSVGVVNGVPLLDLDYPEDSGCDSDVNIVMTASGKIIEIQGTAEDAPFSLDELG
                     KLVALAQKGIGELLQHQQNALSAA"
     gene            complement(3030..3494)
                     /locus_tag="WGQ68_001568"
     CDS             complement(3030..3494)
                     /locus_tag="WGQ68_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEZ0715343.1"
                     /translation="MYKHLVVAVDGSETSINALKHAAELAGVNSARLTLVHVANPAEY
                     MALAPEFLQHESYEAAAVAQGNEVLDAAERTARELGVENTVKHLLVANKGAREMAQDL
                     VDYADENGADLLVLGTHGRTGLMHLLMGSFAETVMRQSHLPLLIIRSKAEEA"
     gene            3711..4487
                     /locus_tag="WGQ68_001569"
     CDS             3711..4487
                     /locus_tag="WGQ68_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HEZ0715344.1"
                     /translation="MSNNALTSSRRFGGIARLYGDSALAHFSQAHVCVVGVGGVGSWA
                     VEALARTGIGRLTLIDLDNVAESNVNRQLHALTGDFGKAKVTALRERVAQINPECQVF
                     EIEDFVTEDNLPEYFGKGFDFVIDAIDQVRVKAAMAAYFVERKQPFVLSGGAGGQKNP
                     ALIQTADLSRVTHDPLLANLRYTLRKRYGFSRDTKEKMRVPCVFSTENIVPPQSGVAC
                     SADAAPQGLSCAGYGASMLVTASFGLYCAQAAVEHIAGKK"
     gene            4732..5586
                     /locus_tag="WGQ68_001570"
     CDS             4732..5586
                     /locus_tag="WGQ68_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEZ0715345.1"
                     /translation="MPPIPAPSAPSEHTVAWVFGQPVTDLPQDLFIPPDALKVVLGSF
                     QGPLDLLLYLIRKQNIDVLDIPMVKITEQYLHYIAQIETYQFDLAAEYLLMAAMLIEI
                     KSRLLLPRTEAVEDEEADPRAELVRRLLAYEQMKLAAQGLDALPRAGRDFAWAYLPLE
                     IAVEAKLPEVYITDLTQAWLGILSRAKHTRSHEVIKETISVRAQMTAILRRLNGHGIC
                     RFHDLFNPEQGAAYVVVNFIALLELAKEGLVGIVQEVGFGEIRISLNHEGAHSDGISG
                     TRGGRDVF"
     gene            5627..6835
                     /gene="pncB"
                     /locus_tag="WGQ68_001571"
     CDS             5627..6835
                     /gene="pncB"
                     /locus_tag="WGQ68_001571"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEZ0715346.1"
                     /translation="MTGIIHSLLDTDLYKFTMLQVVLHQFPQTHSLYEFRCRNASTVY
                     PLADIKEDLEVELDALCQLRFTHDELGYLRSLRFIKSDFVDYLELFQLQRRFVEIGTD
                     DKDRLNIRIEGPMIQAMFFEIFILAIVNELYFRRLETPAVIEEGERRLQAKAVRLKEI
                     AAAQNPDDPPFLISDFGTRRRYKLAWQEHVIRTLLEAAPSIVRGTSNVFLAKKLGITP
                     IGTMAHEFLQAFQALDVRLRNFQKAALESWVHEYRGDLGVALTDVVGMDAFLRDFDLY
                     FAKLFDGLRHDSGDPYIWGDKAYAHYQKLKIDSRTKMLTFSDGLDIERSWALHQYFKD
                     RFKTGFGIGTNLTNDMGHTPLNIVLKLVECNGQSVAKLSDSPGKTMTNNSTFLAYLRQ
                     VFDVPEPETP"
     gene            6927..8645
                     /gene="argS"
                     /locus_tag="WGQ68_001572"
     CDS             6927..8645
                     /gene="argS"
                     /locus_tag="WGQ68_001572"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEZ0715347.1"
                     /translation="MNLHQTVEREAAAAFAAAGIADSPVVLQPTKNAEHGDFQINGVM
                     GAAKKAKQNPRELAQKVAEALADNAVIESAEVAGPGFINLRLRPDFLAQNIQTALNDT
                     RFGVAKTDKPQTVVIDYSSPNLAKEMHVGHLRSSIIGDSISRVLEFMGNTVIRQNHVG
                     DWGTQFGMLVAYLVEQQKDNAAFELADLEQFYRAAKVRFDEAPAFADTAREYVVKLQG
                     GDETVLALWKQFVDISLSHAQAVYDTLGLKLRPEDVAGESKYNDDLQPVVDDLVQKGL
                     AVEDDGAKVVFLDEFKNKEGEPAAFIVQKQGGGFLYASTDLACLRYRVGTLHADRLLY
                     VVDHRQALHFEQLFTTSRKAGYLPENVGAAFVGFGTMMGKDGKPFKTRSGDTVKLVDL
                     LTEAVERATALVKEKNPELGADEAAKIGKTVGIGAVKYADLSKNRTSDYVFDWDAMLS
                     FEGNTAPYLQYAYTRVQSVFRKAGEWDADAPTVLTEPLEKQLAAELLKFENVLQSVAD
                     TAYPHYLAAYLYQIATLFSRFYEACPILKAEGASRNSRLQLAKLTGDTLKQGLDLLGI
                     DVLDVM"
     gene            9203..10621
                     /locus_tag="WGQ68_001573"
     CDS             9203..10621
                     /locus_tag="WGQ68_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEZ0715348.1"
                     /translation="MNIHALLSKQWTLPPFLPKRLLLSLLILLAPNAMFWVLALLTAT
                     ARPIVNLDYLPAALLIALPWRFVKIAGVLAFWLAVLFDGLMMVIQLFPFMDLIGAINL
                     VPFILTAPTPYQIMTGLLLLYMLAMPFVLQKAAAKTDFRHIAVCAAVVAAAGYFTGHL
                     SYYDRGRMANIFGANNFYYAKSQAMLYTVSQNADFITAGLVDPVFLPLGNQQRAATHL
                     NEPKSQKILFIVAESWGLPANPELQNATFAKLLAQKDRFSVWESGSFPFIGATVEGEM
                     RELCAYGGLRGFALRRAPDEKFARCLPNRLKQEGYATFAMHGAGSSLYDRFSWYPRAG
                     FQEIKTAENLIGKKTCAIFGGVCDSELFGEVSAFFKKHDKGLFYWMTLTSHADYPESD
                     IFNHRLKCTEYGLPAETDLCRNFSLHTQFFDQLADLIQRPEMKGVEVIIVGDHPPPVG
                     NLNETFRYLKQGHVAWLHFKIK"
BASE COUNT         2515 a         3140 c         2746 g         2491 t
ORIGIN      
        1 ccccaaccta aaatccgtca ttcccgcgca ggcgggaatc tagacattca atgctaaggc
       61 aatttatcgg aaatgactga aactcaaaaa actggattcc ctctttcgtg ggaatgacgt
      121 agtgcaggtt tccgtacgga tggattcgtc attcccgcgc aggcgggaat ctagacattc
      181 aatgctaagg caatttatcg ggagtgactg aaactcaaaa aactggattc ccgctttcgt
      241 gggaatgacg cgattagagt ttcaaaattt attctaaata gctgaaactc aacgcactgg
      301 attcccgcct gagcgggaat gacgaatttc aggttgctgt ttttggtttt ctgtttttgt
      361 gaaaataatg ggattttagc ttgtgggtat ttaccggaaa aaacagaaac cgctccgccg
      421 tcattcccgc gcaggcggga atctagtccg ttcggtttcg gtttttttgg ctagtgccgc
      481 aacattaaat ttctagattc ccactttcgt gggaatgacg ggatgtatag tggattaaca
      541 aaaccagtac ggcgttgcct cgccttagct caaagagaac gattctctaa ggtgctgaag
      601 caccaagtga atcagttccg tactatttgt actgtctgcg gcttcgtcgc cttgtcctga
      661 tttttgttaa tccactataa atttaatcca ctatattttt tgttccaaag tcaaaatatg
      721 ccgtccgaac attcgggcgg cagacaaaac ggcactgccc gataaaggca gtgccgttgt
      781 ccgtttcaaa ccgtgaaaca tcagcccaaa ttaaaggctt tatgcaatac cctggttgcc
      841 agttccatgt atttttcatc aatcaatacg gaaactttga tttcggaggt ggaaatcatt
      901 tggatgttga taccctcttc ggcgagcgtg cggaagattt tggcggctac accgacgtgc
      961 gaacgcatac ccaaaccgac tgcggagact ttgcacacgg tgtcgtcgcc atcaatagaa
     1021 gccgcgccga tactgtcttg gcgttccgac aggatttcca aagtctgctt gtaatcgccg
     1081 cgcggtacgg taaaggaaaa atcggttgtg ccttcgctgc cgacattttg gataatcata
     1141 tcgacttcga tgttggcatc ggcgaccgcg cccaaaatct gataggcgac accgggtttg
     1201 tcgggtacgc cgcgcacgtt gatgcgggct tggtttttgt cgaatgcgat accggttacg
     1261 gcagctcttt ccatgttgtc gtcctcttca aaggtaatta aggtgccgtt gccgccgtct
     1321 tgcaggctgc tcagtacgcg caggcgcact ttgtattttc cggcaaattc tactgaacgg
     1381 atttgcaaaa ctttcgaacc gaggcttgcc agttcgatca tttcttcaaa tgtaaccgta
     1441 tccatgcggc gcgcttcggg tacgacgcgg gggtcggttg tgtacacgcc gtccacgtcg
     1501 gtatagattt ggcattcgtc cgctttgagg gctgcagcaa gcgcaacggc ggaagtgtcg
     1561 gaaccgccgc gtccgagcgt ggaaatatcg ccttcactgc tgatgccttg gaagccggca
     1621 acgatgacga ctttgcctgc atcgaggtcg gcacgcattt tttcgtcatc aatgctttcg
     1681 atgcgggctt tggtgtgggc ggtatcggtt ttgagggcga cctgccagcc tgtgtagctt
     1741 ttggcatcca cgccgatgtc tttcaatgcc atcgccaaaa ggccgatggt tacttgttcg
     1801 ccggtggaca aaaccacgtc caattcgcgc ggatcgggat gctcctgcat ttcgtgcgcc
     1861 aacgcgacca ggcggttggt ttcaccgctc atggcggata cgacgacgac aatgtcgtgt
     1921 ccttcggcgc gggctttggc gacacgtttg gccacgtttt taatgcgttc gggcgagcct
     1981 acggatgtgc cgccgtattt gtgtacgatt aacgccatgt ttcgtgcttt cttgtgtggg
     2041 attggtcggg cagcatggtt tgctggaaaa gcggctatta ttactatttt ttacatgaag
     2101 ttcaagaacg gactgcgctt tcccgcctgc cgtttgacag cggtcagcga aaaacctgtt
     2161 ctttcagatt gttgacaaaa tgccgtctga acggttttca gacggcatcc ggacgacaat
     2221 caggcggcgg acaacgcatt ttgctggtgt tgcagcagtt cgcctatgcc tttttgcgcc
     2281 agtgcaacca gtttgcccaa ttcgtccaaa ctgaacggcg cgtcttccgc cgtaccttgg
     2341 atttcgatga tttttcccga tgcggtcatg acgatattca catcactgtc gcaaccggag
     2401 tcttcgggat aatccaaatc caaaagcggc acgccgttca ctacgcctac tgacacagcg
     2461 gcaacggctt cgcggatggg gttttcactc aaaatgccgt ctgaaaccag tttgccgacg
     2521 gcgatttgca gcgcgacaaa cgcaccagta atcgaagccg tgcgcgtacc gccgtctgcc
     2581 tgaatcacgt cgcagtcaat caagatttgg cgttcgccga gtttttccat atccacgacc
     2641 gcgcgcagcg aacgcccgat aaggcgttgg atttcttgtg tgcgcccgga ctgtttgccc
     2701 gccgaagctt cgcggcgcat ccgggaagca gttgaggcag gcagcatccc gtattctgcg
     2761 gttacccagc cttggttttt gccgcgcaaa aacggtggga cattttcgtc tatggaagcg
     2821 gtacaaatca ctttggtatt accgcattca ataaggcacg aaccgtcggc gtgcggcagg
     2881 aaatgagggg tgattttgat atcgcgcagg ctgtcggcgg cgcgcgagat gcggatgtaa
     2941 tcaggcatac tgccctcccg ttaaaaacag ataaattaaa aagccttaaa tatgaaaaat
     3001 cacatttaag gccttcaaac tgaaaatttc tacgcctctt cggctttgct gcggataatc
     3061 aaaagcggca ggtggctttg gcgcattacc gtttcggcaa aactgcccat taaaaggtgc
     3121 atcagcccgg tacgtccgtg cgtacccaac accagcaagt cggcaccgtt ttcatcggca
     3181 taatcaacca aatcctgcgc catttcacgc gcacccttat tggcaaccag caggtgtttg
     3241 acggtatttt ccacacccag ttcccgggcg gtgcgctcgg cggcatccaa aacttcgttg
     3301 ccttgcgcga cggcggcggc ttcgtagctt tcgtgttgca aaaattcggg ggcgagcgcc
     3361 atatattcgg caggattggc aacgtgcacc aaagtcaggc gcgcgctgtt gactcctgca
     3421 agctcggcgg catgtttcag ggcattgatg gacgtttcac tgccgtcaac ggcaacgacc
     3481 agatgtttgt acatatcgta ttctcctttt gcaccgcctc gcggtgccct cttgtcggat
     3541 gggcgcaggg acagtttgcg ccgtttcatt atagacccgc cgtcgggctt tatacaacag
     3601 ccgaacagcc cgaccgcttt ccagtataat atgccgcttc cgtgcagtca ggcatttttt
     3661 gccggctttc gttcactttt tgatttgacg caatcttgca ggattcgacc atgtccaaca
     3721 acgctttgac ctcttcgcga cgcttcggcg gcatcgccag actctacgga gactctgcct
     3781 tggcgcactt ttcacaggca cacgtctgcg tggtcggcgt gggcggtgtc ggctcgtggg
     3841 cggtcgaggc tttggcgcgc acgggcatcg ggcgtttgac tttgattgat ttggacaacg
     3901 ttgccgaatc gaatgtcaac cgccagctgc acgccctgac cggagacttc ggcaaagcaa
     3961 aagttaccgc cttgcgcgaa cgcgtcgccc aaatcaaccc agaatgccaa gtgtttgaaa
     4021 ttgaagattt cgttaccgaa gacaatttgc cggaatactt cggaaaaggt tttgatttcg
     4081 tcattgacgc gatcgaccaa gtgcgcgtca aagcggcaat ggcggcttat tttgtggaac
     4141 gcaaacaacc gtttgtcctc agcggcggcg caggcggaca aaaaaatccg gcgttaatcc
     4201 aaaccgccga tttgagccgc gtaacccacg atccgctgct tgccaacctg cgctacacct
     4261 tgcggaaacg ctacggcttc agccgcgata ctaaggaaaa aatgcgcgtg ccgtgcgtgt
     4321 tctcaaccga aaacatcgta ccgcctcaat cgggcgtggc gtgttctgcc gatgccgcac
     4381 cgcagggctt gtcgtgcgcc ggctatggcg caagtatgct cgttaccgct tcgttcggac
     4441 tgtattgcgc acaggcggcg gtggaacaca tcgcgggcaa aaaataagca atgccgtctg
     4501 aaacaggatt cagacggcat ttgaacaaac gcggcaatca agctgtcggt tcagaaaaac
     4561 caacatcggc acgcctaaaa ccgtattcac aacctgctcc ttttcaaaac atttgcattt
     4621 aaaagccgtt ataatgccgt ctgaacatct gcccgaccac attatacgtg aatgtcggca
     4681 gattgttttc ttttgtaaac ttatattaaa atccacttac cgattcacgc catgccgccc
     4741 atccctgccc catctgcacc atccgagcac actgtcgcat gggtattcgg ccaacccgtt
     4801 accgatttgc cgcaggattt gtttatcccg cccgacgcgc tgaaagtcgt attgggcagt
     4861 tttcaaggcc ctttggatct actgctgtat ctgatccgca agcagaacat cgatgttctc
     4921 gatattccga tggtgaagat taccgagcag tatctgcact acatcgccca aatagaaacc
     4981 tatcagtttg atttggcggc ggaatatctt ttgatggcag caatgctgat tgaaatcaaa
     5041 tcgcgcctgc tgctgccgcg taccgaagcc gtcgaagacg aagaagccga cccacgtgcc
     5101 gagttggtgc gccgcctgct ggcttacgag cagatgaagc tggcggcaca agggttggat
     5161 gcgctgcccc gagccggacg ggatttcgcg tgggcttacc tgccgctgga aattgccgtc
     5221 gaagccaagc tgcccgaagt ctatattacc gacttgacgc aagcgtggct gggtattttg
     5281 tctcgggcaa aacatacgcg cagccacgaa gtaatcaaag aaaccatctc cgtgcgcgcg
     5341 caaatgacgg caatcctgcg ccgtttgaac ggacacggga tatgcaggtt tcacgacctg
     5401 ttcaatcccg aacagggcgc ggcttacgtg gtcgtcaact tcatcgcact gttggagctt
     5461 gccaaagaag gtttggtcgg aatcgtacag gaagtcggtt tcggggaaat ccgaatcagc
     5521 ctcaatcatg agggggcgca ttcagacggc atttccggca cacggggcgg gcgcgatgtg
     5581 ttctaatacg ccccaagccg ccaccaaaaa tcgggagaca cgccatatga ccggcatcat
     5641 acattcgctg cttgacaccg acctctacaa attcactatg ctgcaagtgg ttctgcacca
     5701 gtttccgcag acgcacagcc tttacgaatt ccgctgccgc aacgcctcga ccgtctatcc
     5761 gcttgccgac atcaaagaag acttggaagt cgaactcgac gcgctctgcc aactgcgctt
     5821 tacccatgac gaactcggct acctccgcag cctgcgtttc atcaaaagcg actttgtcga
     5881 ttatctcgaa ctcttccagc tccaacgccg ctttgtcgaa atcggcacag acgataaaga
     5941 ccgtctgaac atccgcatcg aaggtccgat gatacaggcg atgttttttg aaatcttcat
     6001 cctcgccatt gtcaacgaac tttacttccg ccgcctggaa acccctgcag tcatagaaga
     6061 aggcgaacgc cggcttcaag ccaaagccgt gcgcctcaaa gaaatcgccg ccgcacaaaa
     6121 ccccgacgat ccgcccttcc tgatttccga cttcggcacg cgccgccgct acaagctcgc
     6181 gtggcaggaa cacgtcatcc gcaccctgct cgaagccgcc ccaagcatcg tgcgcggcac
     6241 cagcaatgtc tttctcgcca aaaaactcgg catcaccccc atcggcacca tggcgcacga
     6301 gttcctgcag gcattccagg ccctcgacgt acgcctgcgg aatttccaaa aggccgcgct
     6361 cgaaagctgg gtgcacgaat accggggcga tttgggcgtt gccctgaccg acgtggtcgg
     6421 tatggatgcc ttcctgcgcg atttcgacct ctatttcgcc aaactcttcg acgggctacg
     6481 ccacgacagc ggcgaccctt acatttgggg cgacaaagcc tacgcccact atcaaaagct
     6541 caaaatcgac agccgcacca aaatgctgac cttctccgac gggctggaca tcgaacgctc
     6601 ttgggcatta caccaatatt tcaaagaccg cttcaaaacc ggcttcggca tcggcaccaa
     6661 cctcaccaac gatatggggc atacgccctt gaatatcgtc ttaaaactgg tcgaatgcaa
     6721 cgggcagtcc gtcgccaagc tgtccgactc tccgggcaaa accatgacca acaacagcac
     6781 cttcctcgcc tacctgcgcc aagtgttcga cgtacccgaa cccgaaacgc cgtaaaccgg
     6841 cagaaaaagc gcacaattcc tgtttctgcc gcataaaatc ttttaaaata ccgcctgatt
     6901 tgaatttaac cgaaagaccg aacttcatga acctacatca aaccgtcgaa cgcgaagccg
     6961 ccgccgcctt tgccgccgca ggcatcgccg acagtcccgt tgttttgcag ccgaccaaaa
     7021 atgccgaaca cggcgatttc caaatcaacg gcgtgatggg tgcggcgaaa aaagccaaac
     7081 aaaatccgcg cgaattggcg caaaaagtgg ctgaagcatt ggcggacaat gccgtgattg
     7141 aaagcgcgga agtagccggc ccgggcttta tcaacctgcg tctgcgccct gatttcctcg
     7201 cacaaaacat tcagacagcc ttgaacgaca cgcgtttcgg tgtggcaaaa accgacaaac
     7261 cgcaaactgt cgtcatcgac tattcctcgc ccaatctggc caaagaaatg cacgtcggcc
     7321 atctgcgttc cagcatcatc ggcgacagca tttcgcgcgt attggaattt atgggcaaca
     7381 ccgtcatccg ccaaaaccac gtcggcgact ggggtacgca gttcggcatg ttggtcgctt
     7441 atttggtcga gcagcaaaaa gacaatgccg cgttcgagct ggcggatttg gagcagtttt
     7501 accgcgccgc caaagtgcgc tttgacgaag cccctgcctt tgccgacacc gcgcgcgaat
     7561 acgttgtgaa gctgcaaggc ggcgatgaaa ccgttttggc attgtggaaa cagtttgtcg
     7621 atatttcgct ctcgcacgcc caagccgttt acgacacgct gggcttgaag ctgcgccccg
     7681 aagacgtggc gggcgaatcg aaatacaacg acgatttgca gcccgtagtc gatgatttgg
     7741 ttcaaaaagg tctggcagtc gaagacgacg gcgcaaaagt cgtgttcttg gacgagttta
     7801 aaaacaaaga aggcgaaccc gccgcattta tcgtgcaaaa acaaggcggc ggcttccttt
     7861 atgcttccac cgatttggcg tgtctgcgct accgtgtcgg cacgcttcat gccgaccgcc
     7921 tgctgtacgt cgtcgaccac cgccaagccc tgcactttga gcaactcttt accacttcac
     7981 gcaaagccgg ctatctgccg gaaaacgtcg gcgcggcatt tgtcggcttc ggcacgatga
     8041 tgggcaaaga cggcaaaccg ttcaaaacac gcagcggcga caccgtgaaa ctggtcgatc
     8101 tgctgaccga agccgtcgaa cgcgccaccg ctttggtgaa agaaaaaaat cccgaattgg
     8161 gtgcagacga agcggcaaaa ataggcaaaa ccgtcggcat cggcgcggtc aaatacgccg
     8221 acttgagcaa aaaccgcacc agcgattatg tgttcgactg ggacgccatg ctctcgtttg
     8281 aaggcaacac cgccccctat ctgcaatacg cctacacccg cgtgcaaagc gtgttccgca
     8341 aagcaggcga atgggacgcg gacgcgccaa ccgttttgac cgaacctttg gaaaaacaac
     8401 tggccgccga gcttttgaaa tttgaaaacg tgctgcaaag cgtggcggac acggcgtatc
     8461 cgcactacct cgccgcctac ctctatcaaa tcgctacctt gttcagccgc ttctacgaag
     8521 cctgcccgat tttgaaagcc gaaggcgcaa gccgcaacag ccgcctgcaa ctggcgaagc
     8581 tcaccggcga cacgctgaaa caaggcttgg atttgctggg catcgatgtg ttggacgtaa
     8641 tgtaaaaccg caccgcccga ttgcggacaa cagccccgca atcaaccggc ggaccatcct
     8701 tatccgaatc tgaaaaaagc ggcgcgatac accgtatccg ccgcctccca aaatgcgaaa
     8761 cagacaaacg ccaagcaagc aagcaagcaa gcaagcaagc aagcaagcaa aaaattataa
     8821 cccccttcct gccgacgcac gcactttccg cgcggcgcgt tccccttttc ccgctcctca
     8881 aacccgcctt ttcttcaggc agggtttcag cccgcctcct ttccctgttt tccttccctc
     8941 aacacgcgtg cgcgccccct gccgcaccgt gccgcacttt cgcgcccgga cggcatcggt
     9001 ctgtcatccg gttctctgtt ttaaataccc ctgtttcaga aagaaattgc agatgtttca
     9061 acacacagga cgacacataa agcaccgccc tatgtgttgc cctgatttgg aaggggttgc
     9121 gcccctccca aataaagtct gatcctgccg ccccgaagga cagatgtccg agtggcgggg
     9181 tttcaaccga aaaggaaata cgatgaatat tcacgccctg ctctccaaac aatggacgct
     9241 gccgccattc ctgccgaaac ggctgctgct gtccctgctg atactgctgg cccccaatgc
     9301 gatgttttgg gttttggcac tgctgaccgc caccgcccgc ccgattgtca atttggacta
     9361 ccttcccgcc gcgctgctga tcgccctgcc ttggcgtttc gtcaaaattg ccggcgtatt
     9421 ggcgttttgg ctggcggttt tgtttgacgg gctgatgatg gtgatccagc ttttcccttt
     9481 tatggacctc atcggcgcca tcaacctcgt ccccttcatc ctgaccgccc ccacccctta
     9541 tcagataatg accgggctgt tgctgctgta tatgctggcg atgccgtttg tgttgcagaa
     9601 agccgccgcc aaaaccgact tccggcacat tgccgtctgc gccgccgttg tggcggcagc
     9661 cggctatttc accggccatt tgagttacta cgaccggggg cggatggcca atatcttcgg
     9721 cgcaaacaac ttctattacg ccaaaagtca ggcgatgctc tacaccgtta gccagaatgc
     9781 cgactttatt accgccggcc tggtcgatcc cgtcttcctc cccttgggca atcagcagcg
     9841 tgccgccacg catctgaacg agccgaaatc tcaaaaaatc ctctttatcg tcgccgaatc
     9901 ttgggggctg ccggccaatc ccgaacttca aaacgccact tttgccaaac tgctggcgca
     9961 aaaagaccgt ttttcggttt gggaaagcgg cagttttccc tttatcggcg cgacggtcga
    10021 aggcgaaatg cgcgaactgt gtgcctacgg cggtttgcgc gggtttgcac tgcgccgcgc
    10081 gcccgacgaa aaatttgccc gctgcctccc caaccgtttg aaacaagaag gttacgccac
    10141 ctttgcgatg cacggcgcgg gcagttcgct ttacgaccgc ttcagctggt atccgagggc
    10201 gggctttcaa gaaatcaaaa ccgccgaaaa cctgatcggt aaaaaaacct gtgccatttt
    10261 cggcggcgtg tgcgacagcg agctgttcgg cgaagtgtcg gcatttttca aaaaacacga
    10321 caagggactg ttttactgga tgacgctgac cagccacgcc gactatcccg aatccgacat
    10381 tttcaaccac aggctcaaat gcaccgaata cggcctgccc gccgaaaccg acctctgccg
    10441 caatttcagc ctgcacaccc aattcttcga ccaactggcg gatttaatcc aacgccccga
    10501 aatgaaaggc gtggaagtca tcatcgtcgg cgaccatccg ccgcccgtcg gtaacctcaa
    10561 tgaaaccttc cgctacctca aacaggggca cgtcgcctgg ctgcacttca aaatcaaata
    10621 acaacaatgc cgtctgaacg caccaacagc cttcagacgg cattttgcag acagaccgac
    10681 ccttcaagcc cacttttttc atcatctccg ataaattgct ttgtatagtg gattaacaaa
    10741 aaccagtaca gcgttgcctc gccttagctc aaagagaacg attctctaag gtgctgaagc
    10801 accgagtgaa tcggttccgt actatttgta ctgtctgcgg cttcgtcgcc ttgtcctgat
    10861 ttttgttaat ccgccataaa gaccgtcggg ca
//
LOCUS       DAWFHY010000070        10228 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.070, whole genome shotgun sequence.
ACCESSION   DAWFHY010000070 DAWFHY010000000
VERSION     DAWFHY010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.070"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(78..152)
                     /locus_tag="WGQ68_001574"
     tRNA            complement(78..152)
                     /locus_tag="WGQ68_001574"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(117..119),aa:Glu,seq:ttc)
     gene            complement(170..246)
                     /locus_tag="WGQ68_001575"
     tRNA            complement(170..246)
                     /locus_tag="WGQ68_001575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(210..212),aa:Arg,seq:acg)
     gene            complement(356..2029)
                     /gene="recN"
                     /locus_tag="WGQ68_001576"
     CDS             complement(356..2029)
                     /gene="recN"
                     /locus_tag="WGQ68_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEZ0715349.1"
                     /translation="MLLTLSLRDFVIVENLNLDFQSGFTVLTGETGAGKSITLDAIGL
                     LLGDKADYSQVRSGAKEAQLSALFDISHLPVLKAELYEQGLLNDGEEELSIRRIIDAK
                     GKSRSFINNQAATLAQLKAVGSQLIDIHGQNAHHSLNQEAAQRELLDAFAGSRAQAET
                     VRQLYQNWANSKKALQEAQEQAENIALEQERLEWQFNELNQLDIKQGEWEALSQSHDS
                     LAHSAELLQAAEEVGSKIDGDNGIQRHIYQCQKLLANLQNIEPRFAESLNMLASIEAE
                     LGEISANMRDVAGRSDINPNELAAQEQRMGELMGMARKYRIEPEELPAKLAEIEERLQ
                     SLQAAADLDALEHNVAHNFAEYQEAAHILSAMRHQAAERLSGETTEHMQHLAMKGARF
                     DIVLLPSSPTAHGLEQVQFQVAANKGNPPRPLNKVASGGELARISLALQVVASQYTQV
                     PTLIFDEVDTGIGGGVAEMVGKALRALGRKHQVLAVTHLPQVASCGENHWRVRKHSEG
                     EQTVSEISILDEIQRIEEVARMLGGEVITDTTRQHAAELLQLASKNSLF"
     gene            complement(2213..2815)
                     /locus_tag="WGQ68_001577"
     CDS             complement(2213..2815)
                     /locus_tag="WGQ68_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003749777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEZ0715350.1"
                     /translation="MTDKISPDALIEAALLTQTEPLTEKSMRELCVPPLSQDKLIDVL
                     AQLKTRWQDRALQLVHTQEGWRFQIVQTAFERLGSLQEQRAPRYSRAVMETLAIIAYQ
                     QPVTRGDIEGIRGVAVSQNVMQTLQDRGWIEVIGHRDTLGKPALWATTATFLSDLRLD
                     GLEELPPLTELGELVLPDLMEMPPTDEEEPEAVPSDTLPN"
     gene            complement(2916..3857)
                     /gene="ylqF"
                     /locus_tag="WGQ68_001578"
     CDS             complement(2916..3857)
                     /gene="ylqF"
                     /locus_tag="WGQ68_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEZ0715351.1"
                     /translation="MAIQWFPGHMNKAKKAIAERAKSVDMVIEMLDARMPASSENPLL
                     AQLSKGKPKLKILNKQDLADPERTKIWLEHYNSRPDTCAIALDSSETGAHGKITQACR
                     AMIPHRQGIDKPLRVLICGIPNVGKSTLINGMIGKKSAKTGNEPGITKAEQRLFLADD
                     FWLYDTPGMLWPKIIVEEGGYNLAAGGAVGRNALDEEEVALELLDYLRRHYLPMLQER
                     YQADKDPSSHWDENVWLEWIAKKRGAVLSGGRINYQKAAENILTDFREGKIGRITLET
                     PNQWETWLKKARQKEAELKAIREARKAERKGQKPSEA"
     gene            complement(3938..4792)
                     /gene="rapZ"
                     /locus_tag="WGQ68_001579"
     CDS             complement(3938..4792)
                     /gene="rapZ"
                     /locus_tag="WGQ68_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEZ0715352.1"
                     /translation="MKIVLISGLSGSGKSVALRQMEDSGYFCVDNLPLEMLPALVSYH
                     IERADETELAVSVDVRSGIDIGQAREQIAYLRGLGHRVEVLFVEAEESVLVRRFSETR
                     RGHPLSNQDMTLLESLKKEREWLFPLKEIAYCIDTSKMNAQQLRHAVRQWLKVERTGL
                     LVILESFGFKYGVPNNADFMFDMRSLPNPYYDPELRPYTGMDKPVWDYLDGQPLVQEM
                     VDDIEKFLTHWLPRLEDESRSYVTVAIGCTGGQHRSVYVVEKLARRLKGRYELLIRHR
                     QAQNLPSR"
     gene            complement(4773..5735)
                     /locus_tag="WGQ68_001580"
     CDS             complement(4773..5735)
                     /locus_tag="WGQ68_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEZ0715353.1"
                     /translation="MPSISVRRLFDDNQYKLQLAWAAGNSGADNRIGVEADKPVLALV
                     GHLNFIHPNQIQVVGLAESEYLNRLESGETGYQFGNLFDISMSLVIVANGLPVSPGLR
                     DYCHKNDIPLLTSKLESPYLMDVLRIYLQRTLAASSVKHGVFLDVFEIGVLITGHSGL
                     GKSELALELISRGHSLIADDAVELFRIGPETLEGRCSPMLRDFLEVRGLGILNIRHIF
                     GETSIRPKKILQLIINLVEADDEYMKQLDRLSIRTETESILNVNVRSVTLPVAVGRNL
                     AVLVEAAVRNYILQLRGKDSTREFLERHQTQLKENEQNHENRPD"
     gene            complement(5737..6186)
                     /gene="ptsN"
                     /locus_tag="WGQ68_001581"
     CDS             complement(5737..6186)
                     /gene="ptsN"
                     /locus_tag="WGQ68_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HEZ0715354.1"
                     /translation="MSLIGEILPLSHIVLDMEVGSKKRLFEEAGLLLERESSLFHADV
                     FECLFAREKLGSTGLGQGVAIPHGRHAGVMQATGAFIRTREPVGFDAPDGKPVSLVFI
                     LLVPENATGEHLEVLSKLAGKFSQKSIRESLMTVSSAEEARAILTEE"
     gene            complement(6371..7261)
                     /gene="ubiA"
                     /locus_tag="WGQ68_001582"
     CDS             complement(6371..7261)
                     /gene="ubiA"
                     /locus_tag="WGQ68_001582"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HEZ0715355.1"
                     /translation="MNPKSPLFLRLSDRLDVYLRLMRADKPIGTLLLLWPTYWALWLA
                     SDGIPDLAVLAAFTIGTFLMRSAGCVINDFADRDFDGAVERTKNRPFAQGRVKKKEAL
                     LLTAFLCLLAALCLIPLNHLTWLMSLPALFLALTYPFTKRFFPIPQLYLGLAFSFGIP
                     MAFAAVAGNVPPQAWILFAANVLWTLAYDTVYAMADKEDDLKIGIKTSAVTFGRYDIA
                     AVMLCHGGFTLLMAVLGAVIGAAWAYWTAIPIVLLLQYRQYAAIKSRVRQICFETFLA
                     NNRIGWVWFAAIFAHTFFAK"
     gene            complement(7291..7767)
                     /locus_tag="WGQ68_001583"
     CDS             complement(7291..7767)
                     /locus_tag="WGQ68_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HEZ0715356.1"
                     /translation="MEHLFGKWLPDLPADVSDGISLPMVRLLHTRSLTAALRALPHTF
                     SVELLKLGELETECGGRLVREVLLKLDGIPVVAARSECRIGSAFWQNILDCGTRPLGE
                     RLFQADLEGARSAFEFAVSGEGCGRYFAARRSRFSRHGEEMLLTEYFLPELKRFIG"
     gene            complement(7782..8429)
                     /gene="bioD"
                     /locus_tag="WGQ68_001584"
     CDS             complement(7782..8429)
                     /gene="bioD"
                     /locus_tag="WGQ68_001584"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HEZ0715357.1"
                     /translation="MKGVYFVSGIDTDIGKTVATGVLAKQLLQQGKSVITQKPVQTGC
                     QNIADDIAVHRKIMGIPMQEADKRRLTMPEIFSYPASPHLAARLDGRALDLDKIRTAT
                     QELAAQYEVVLVEGAGGLMVPLTEKLLTIDYIRQQGYPVILVTSGRLGSINHTLLSFA
                     ALKQYGIRLYSLVFNHIHDSRDAHIAQDSLNYLKCRLKADFSEAEWMELAKTDAV"
     gene            complement(8426..9727)
                     /gene="bioA"
                     /locus_tag="WGQ68_001585"
     CDS             complement(8426..9727)
                     /gene="bioA"
                     /locus_tag="WGQ68_001585"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HEZ0715358.1"
                     /translation="MPSEHQHTSSLLDFDRTHLLHPYTSMTDPLPVYPVKRAEGVFIE
                     LADGTRLIDGMSSWWCAIHGYNHPVLNQAVETQMKQMSHVMFGGLTHEPAVELGKLLV
                     GILPQGLDRIFYADSGSVSVEVALKMAVQYQQARGLRAKQNMATVRRGYHGDTWNAMS
                     VCDPETGMHHIFGSALPQRYFVDNLTSRFDDEWDEADLQPVRALFEAHHTDIAAFILE
                     PVVQGAGGMYFYHPQYLRGLRDLCDEFDIVLIFDEIATGFGRTGKMFACEHAEVVPDI
                     MCIGKGLSGGYMTLAAAITSQKVTETISRGEAGVFMHGPTFMANPLACAVACASVKLL
                     LSQDWQANIRRIESILKCRLKTAWDIRGVKDVRVLGAIGVIELEKGVDMARFQADCVA
                     QGIWVRPFGRLVYLMPPYIISDGVLTKLADKTVQILKEHSK"
BASE COUNT         2372 a         2796 c         2587 g         2473 t
ORIGIN      
        1 gaaatgagaa atgagaaatg agaaatgaga aatgagaaat gagaaatgcc tccgatataa
       61 tcggaggcat tttgaattgg cacgcccacg gggaatcgaa ccccggttac cgccgtgaaa
      121 gggcgatgtc ctaaccgcta gacgatgggc gcatattgta ttttcttact ggcgcacccg
      181 gagcgattcg aacgcccgac cctctggttc gtagccagat actctatcca actgagctac
      241 gggtgcagca caatcacttg ttcaagtgat taagaaagcg tgataatacg gatttatctg
      301 agttctgtct attgttttag acttttattt ttagttattt tttaactgat tgattttaaa
      361 ataaactatt tttcgacgca agttgcagca attctgccgc atgttgccgc gtcgtatcgg
      421 taatgacttc tccgcccaac atacgggcaa cctcttcgat ccgttggatt tcatccaata
      481 tactgatttc gctgacggtt tgctctccct cgctgtgctt gcgcacccgc cagtggtttt
      541 ctccgcagga tgcgacttgg ggaaggtggg taacggcaag cacctgatgt tttctgccca
      601 acgcacgtaa tgccttgccg accatttcag ccactccccc tccaataccg gtatcgacct
      661 catcaaaaat cagggtggga acttgggtat attggctggc aacaacctgt aaggcaaggc
      721 tgatacgcgc caattcgccg ccggaggcaa ctttattcag cggacggggc ggattgcctt
      781 tgttggcggc aacttgaaat tgaacctgct ccaaaccgtg tgccgtcggc gacgagggca
      841 acaggacgat gtcgaaacgt gcacctttca tggcaaggtg ttgcatatgc tcggtcgttt
      901 cgccgctcaa acgctctgcc gcctgatggc gcatggcaga aaggatgtgg gcagcttcct
      961 gatattcggc aaaattgtgg gcaacattat gctcgagcgc gtccaaatcg gcggcagctt
     1021 gcaggctttg caggcgttct tcgatttctg ccaacttggc aggcaactct tcaggctcga
     1081 tccggtattt ccgcgccatc cccatcagtt cgcccatacg ctgttcctgt gccgccaatt
     1141 cattggggtt gatgtcgctg cgacctgcca catcgcgcat attggcactg atttcgccca
     1201 attcggcctc gatgcttgcc aacatattca ggctctcggc aaagcgcggc tcgatgtttt
     1261 gcagattggc caatagtttt tgacattgat agatatggcg ttggatgccg ttgtcgccgt
     1321 caatcttgct tccgacttct tcggcagcct gcaacagctc ggcagaatgg gcaaggctgt
     1381 cgtggctttg gctgagggct tcccattcgc cttgtttaat gtccaactga ttcaattcgt
     1441 taaactgcca ttccagacgc tcttgttcga gtgcaatgtt ttctgcctgt tcctgcgctt
     1501 cctgcaatgc tttttttgag tttgcccagt tttgataaag ctgcctgacg gtttccgcct
     1561 gcgccctgct gcccgcaaat gcgtccaaca attcgcgctg agcggcttcc tgattaagcg
     1621 aatgatgggc gttttgcccg tggatgtcga taagctggct gccgacggct ttgagttgcg
     1681 ccaaggtagc ggcctgattg ttgataaagc tgcggctttt gcctttggca tcgataatgc
     1741 ggcggatact gagttcttct tctccgtcgt ttaaaagccc ctgttcatac aattctgctt
     1801 ttaaaacagg taaatgggaa atatcaaaca acgccgacaa ctgcgcttct tttgcgccgc
     1861 tgcggacttg gctgtaatcg gctttatcgc ccaacagcag accaatcgca tccaaagtaa
     1921 tggacttgcc cgcgccagtt tctccggtca atacggtaaa gccgctttga aaatccagat
     1981 tcagattttc gacaatgaca aaatcacgca aagaaagtgt tagaagcatc gtgaggattc
     2041 cttagcaatt ctaccgacaa aagcaatatc aactttagat atacgaatta taactaagat
     2101 tttttaaatt aaaaataagt ttttccttaa ttttgcttag tgcttgtttt tatctgctta
     2161 tttgttgaaa tgccgtctga agcaatgtgc gtttcagacg gcatttggaa tttcagttgg
     2221 gcagggtatc ggacggtacg gcttccggct cttcttcatc cgtaggcggc atttccatta
     2281 aatcgggtaa aaccagttcg cccagttcgg tcagcggcgg aagttcttcc agaccgtcca
     2341 agcgcaaatc gctgagaaaa gttgccgttg tcgcccacaa tgcgggtttt cccaatgtgt
     2401 cccgatgtcc gatgacttca atccaccccc gatcctgcaa ggtctgcatc acgttctgcg
     2461 acaccgccac gccgcgtatg ccctcgatgt cgccgcgcgt tacgggctgc tggtaggcga
     2521 taatcgccag tgtttccatc acggcgcggg agtaacgcgg cgcacgctgt tcttgcaggc
     2581 tgcccagccg ctcgaatgcc gtctgaacaa tctgaaaacg ccagccctct tgcgtatgca
     2641 ccagttgcaa cgccctatcc tgccaacgcg ttttcaactg cgccaacaca tcaatcagtt
     2701 tgtcctgcga cagtggcggc acacacaatt cgcgcataga tttttcggtc agcggttcgg
     2761 tttgggtcag cagtgcggct tcaattagtg cgtcgggaga aattttgtcg gtcatacggg
     2821 tatccgtgct gaaacggcat gggtttggat atgccgtctg aaatcggttg gagtagagag
     2881 aagctgcctg aaaaatattt ttcagacggc attctttatg cttccgaagg cttctgccct
     2941 ttacgctctg cttttctggc ttcgcgtatg gctttgagtt cggcttcttt ctgacgggct
     3001 tttttgagcc aagtttccca ttggttcggc gtttcgaggg tgattctgcc gattttgcct
     3061 tcacggaagt cggtgaggat gttttcggcg gctttttggt agttgatccg tccgccgctg
     3121 aggactgcgc cgcgtttttt ggctatccat tcgagccaaa cgttttcgtc ccagtggctg
     3181 ctggggtctt tgtcggcttg gtagcgttct tgcaacatag ggaggtagtg gcggcggagg
     3241 tagtctaaaa gttcgagggc gacttcttct tcgtccaacg cgttgcgtcc gactgcgccg
     3301 ccggcggcaa ggttgtagcc gccttcttcg acgatgattt tcggccacag cattccgggg
     3361 gtgtcgtaga gccagaagtc atcggcgagg aagaggcgtt gttcggcttt ggtgatgccg
     3421 ggttcgttgc cggttttggc ggattttttg cctatcatgc cgttgatgag ggtggacttg
     3481 ccaacgttgg ggatgccgca gatgaggacg cgcaggggtt tatctatgcc ttggcggtgg
     3541 ggaatcatgg cacgacaggc ttgggtaatt ttgccgtgtg cgcctgtttc ggaggaatcg
     3601 agggcgatgg cgcaggtgtc ggggcggctg ttatagtgtt cgagccagat tttggtgcgc
     3661 tcggggtcgg caagatcttg tttgttgagg attttaagtt taggtttgcc tttggaaagt
     3721 tgggcaagca gggggttttc gctggaggcg ggcattcgcg catccagcat ttcaatcacc
     3781 atgtctacgc ttttggcgcg ctcggcgatg gcttttttcg ctttgttcat atggccggga
     3841 aaccattgga ttgccatgtc tttcctttct tctcaatatt tgaaatgccg tctgaaacag
     3901 aaaatcgggt ttcagacggc atcatcgttc aacgggatta gcggctcggc aggttttgcg
     3961 cctgtctgtg ccgtatcagc aattcataac gccctttcaa ccttcgggcg agtttttcga
     4021 caacatagac ggaacggtgc tgcccgccgg tacagccgat ggcgacggta acgtagctcc
     4081 tgctttcatc ctccaaacgc ggcagccaat gcgtcagaaa tttttcgatg tcgtcaacca
     4141 tttcctgcac aagcggctgt ccgtccaaat aatcccaaac gggcttgtcc ataccggtgt
     4201 aaggcctcaa ctcgggatcg taatacgggt tgggcagact gcgcatatcg aacataaaat
     4261 ccgcgttgtt cggcacgccg tatttgaacc cgaaggactc caaaatcacc agcagccctg
     4321 tacgttcgac cttcagccac tgccggactg catggcggag ctgttgggca ttcatcttgg
     4381 aagtgtcgat acaataggcg atttctttaa gcgggaacag ccattcccgt tctttcttta
     4441 agctttccaa cagggtcata tcctgattgc tcagaggatg tcctcgcctg gtttcggaaa
     4501 accggcggac caacacgctt tcttccgcct cgacaaacaa aacttcaacc ctgtgcccca
     4561 gtccgcgcag ataggcaatc tgttcccgcg cctgtccgat gtcgatgccg gaacgcacat
     4621 cgacgctgac cgccaattca gtttcgtccg cacgctcgat atgatacgac accagcgcgg
     4681 gcaacatttc caaaggcaaa ttgtccacgc agaaataacc cgaatcttcc atttggcgca
     4741 gtgcgacgga tttgcccgaa ccggacaacc cgctaatcag gacgattttc atgattctgt
     4801 tcgttttctt taagttgcgt ctgatggcgt tccaaaaatt cgcgcgtact gtccttaccg
     4861 cgcaactgca aaatgtaatt gcgtaccgcc gcctcaacca aaacggcgag gttgcgtccg
     4921 acggcgacgg gcagcgtaac cgaacggacg ttgacgttga ggatggattc ggtttcggta
     4981 cggatactca aacggtcgag ctgcttcata tactcgtcgt ccgcctcgac caaattgata
     5041 atgagttgca ggattttttt ggggcggatg gaagtttcgc cgaaaatatg gcggatattg
     5101 agtatcccca agccgcgcac ttccaaaaaa tcgcgcagca taggcgaaca ccgcccttcc
     5161 agcgtttccg ggccgatgcg gaacagctcg accgcatcgt cggcaatcag gctgtggccg
     5221 cgcgaaatca gctccaacgc caattcgctc ttacccaaac ccgaatgtcc ggtaatcagc
     5281 acgccgattt caaacacatc gagaaatacg ccgtgtttga cggacgatgc cgccaaggtg
     5341 cgttgcaggt aaatccgcaa cacgtccatc agataggggc tttcgagttt ggaagtcagc
     5401 agtggaatat cgtttttgtg gcagtagtcg cgcagtcccg gggaaaccgg caagccgttt
     5461 gccacaatga ccaaagacat agaaatgtcg aacaggtttc cgaactgata acccgtttcc
     5521 cccgattcga ggcggttcag atattccgat tccgccaaac cgaccacttg gatttggttg
     5581 gggtggatga aattcaggtg tccgaccaaa gcgaggacgg gcttgtccgc ctctacgccg
     5641 atacggttgt ccgcacccga attgccggcg gcccaggcaa gttgtaactt gtattggttg
     5701 tcgtcaaaca gtcggcggac ggaaatactg ggcatattat tcttcagtca aaatggcgcg
     5761 ggcttcttcc gcagaagaaa ccgtcatcag cgattctctg atgctttttt gggaaaactt
     5821 gccggccagt ttggacaata cttccaaatg ctcgcccgtc gcattttccg gaaccagcaa
     5881 gataaaaacc agggaaaccg gtttgccgtc cggtgcgtca aatccgacgg gttcgcgcgt
     5941 acggatgaac gcgcccgtcg cctgcatcac gccggcatga cggccgtgcg ggatggcaac
     6001 gccctgcccc aaaccggtcg aaccgagttt ttcacgggca aaaagacatt cgaaaacatc
     6061 agcatggaac aatgaggatt cgcgttccaa aagcaggccc gcttcctcaa acagcctttt
     6121 tttactgccc acctccatat ccaaaacaat atgggacaaa ggcaaaattt cgccgataag
     6181 gctcataagc ttctcttttc agacatcgca aaacagaaag attgtaccga ctgccagggc
     6241 aaatctcaat cccgcatacg gtatgggctg acataacaca gcgttttaaa aacatatttt
     6301 aacgcttttc ggcacagata gaaatgccgt ccaaagcagt ttacggctct tcagacggca
     6361 ttgccctgcc ttatttcgcg aaaaacgtat gggcaaaaat ggcggcaaac cacacccaac
     6421 caattctgtt gtttgccaaa aacgtttcaa aacagatttg ccggacgcgg cttttgatgg
     6481 cggcatattg gcggtattgc agcagcagga cgatggggat tgccgtccaa tatgcccatg
     6541 ccgcaccgat aaccgcaccc aatactgcca tcagcagggt aaagcctccg tgacacagca
     6601 taacggcggc gatgtcgtaa cgcccgaacg tgacggcgga ggttttgatg ccgattttca
     6661 aatcgtcctc tttgtccgcc atggcgtaaa ccgtgtcata cgccagagtc cataacacat
     6721 tggcggcaaa gagtatccac gcttgaggcg gcacgtttcc ggcaacggcg gcaaacgcca
     6781 tcgggatacc gaaggaaaag gcaagcccga gatagagttg gggaatcgga aaaaaacgtt
     6841 tggtaaacgg gtaagtcagc gcaagaaaca gcgcgggcag gctcatcagc caagtcagat
     6901 gattcagcgg aatcaggcac aatgcggcaa gcaggcacaa aaatgccgtc agcagcagcg
     6961 cttctttttt cttgaccctg ccctgtgcga acggacggtt tttagtgcgc tcgacagccc
     7021 cgtcaaaatc gcggtcggca aaatcgttga tgacgcagcc ggcactgcgc attaaaaacg
     7081 taccgattgt aaacgccgcc aataccgcca aatcgggaat gccgtctgaa gccagccaca
     7141 atgcccagta ggtcggccac agtaaaagca gcgtcccaat gggcttgtcc gcccgcatca
     7201 ggcgcaggta cacatccaaa cggtcggaca gtcgtaaaaa taaaggggat ttaggattca
     7261 tattgccgca cagcttgaaa aaacggtatt ttatccgata aagcgtttca gttcgggcag
     7321 aaaatactcg gtcagcagca tttcctcgcc gtgacgggaa aaccgagaac gccgcgcggc
     7381 aaagtaccgt ccgcatcctt cgccggaaac ggcaaactca aacgccgaac gcgccccttc
     7441 caaatcggct tgaaacagac gctcgcccaa agggcgcgtg ccgcagtcca aaatgttttg
     7501 ccaaaacgcc gaacctatcc ggcactcact ccttgccgcg acaacaggga taccgtccag
     7561 cttcaacaaa acttcgcgca ccagcctccc tccgcattcc gtctccaatt cgcccagttt
     7621 cagcagttcc accgaaaatg tatgcggcaa ggcgcgcaat gcggcggtca gcgaccgggt
     7681 gtgcagcagc cgcaccatcg gcaggctgat gccgtctgaa acgtcggcgg gcaggtcggg
     7741 cagccatttc ccaaataggt gttccatatt tttcccaatc tttataccgc gtctgttttt
     7801 gccaactcca tccattccgc ttcggaaaaa tcggctttca gacggcattt caagtagttc
     7861 aggctgtctt gggcgatgtg tgcgtcgcgg ctgtcgtgga tgtggttgaa cacgaggctg
     7921 tacaagcgaa tgccgtattg tttgagcgcg gcgaaactga gtaaggtgtg gttgatgctg
     7981 cccagccgtc cgctcgttac gagaatgacg ggatagcctt gctgacggat ataatcaatg
     8041 gttaacagct tttccgtcag cggaaccatc aatccgcccg cgccttcgac caaaacgact
     8101 tcgtactgcg ccgccaattc ttgtgtggcg gtgcggattt tgtccaagtc caaagccctg
     8161 ccgtccagtc gggcagcgag atgaggcgaa gctggataac tgaagatttc aggcatggtc
     8221 agccgccgtt tgtcggcttc ctgcatcggt atgcccataa ttttacgatg gacggcgatg
     8281 tcgtcggcaa tgttttggca accggtttgc acgggctttt gcgtaatcac gcttttgccc
     8341 tgctgcaaca attgttttgc caacacgccg gtggcgacgg ttttgccgat gtccgtgtct
     8401 atgccgctga cgaagtaaac gcctttcatt tgctgtgttc cttcaagatt tgcacggttt
     8461 tgtcggcaag tttggtcaaa acgccgtctg aaatgatgta aggcggcatc agatacacca
     8521 acctgccgaa cgggcgcacc caaatgccct gcgccacgca atccgcttga aaacgcgcca
     8581 tatccacgcc tttttccaac tcgatcacgc cgatggcgcc caaaacgcgc acgtctttca
     8641 caccacgaat gtcccacgcg gttttcagac ggcattttaa gatgctttcg atgcggcgga
     8701 tatttgcctg ccaatcttgg gacaaaagca gtttgaccga agcgcaggca acggcacacg
     8761 ccagcgggtt cgccataaat gtcgggccgt gcatgaacac gcccgcttct ccgcgcgaaa
     8821 tcgtttcggt aactttttgc gaagtgattg ctgccgccag cgtcatatag ccgccgctca
     8881 aacccttgcc gatgcacata atatccggca cgacctccgc gtgttcgcag gcaaacatct
     8941 tgcccgtgcg cccgaatccg gtggcgattt catcaaaaat caaaacgata tcaaactcgt
     9001 cgcacaaatc gcgcaatccg cgaagatact gcggatgata aaaatacata ccgcccgcgc
     9061 cctgcacgac cggttccaaa ataaaggcgg caatgtccgt atgatgcgct tcaaacaagg
     9121 cgcggacagg ctgcaaatcc gcctcgtccc attcatcgtc gaaacggctt gtcaggttat
     9181 cgacaaaata acgctgcggc aacgcgctgc cgaaaatatg gtgcatcccc gtttccggat
     9241 cgcagacgga catcgcgttc caagtatcgc cgtgataccc gcgccgcacc gtcgccatat
     9301 tttgttttgc acgcaaaccc cgcgcctgct ggtattgcac cgccatcttc aacgcgactt
     9361 ccaccgaaac cgaacccgaa tccgcataaa aaatacgatc cagcccctgc ggcaaaatcc
     9421 cgaccaacaa tttgcccagc tccaccgccg gctcatgcgt caaaccgccg aacatcacat
     9481 gcgacatctg tttcatctgc gtctcaaccg cctgattcaa aacgggatga ttgtaaccgt
     9541 gtatcgcaca ccaccaagac gacatcccgt caatcagccg cgtgccgtcc gccaattcga
     9601 taaacacccc ttctgcacgt ttgacaggat aaacgggaag cggatcggtc atggaagtat
     9661 agggatgaag cagatgggta cggtcgaaat caagcaatga tgatgtgtgt tgatgttcag
     9721 acggcataag tttctctctt ttcttcttac tgtattcaaa cgcaaacgcg tattctactc
     9781 cgacagaccg cttcccacac ctttccatcc gtttcgggcg caaaaccgcg aaacaaatcg
     9841 tccgcagcat aagcgcacac cgtttcgcat tcctcaagcc tgattggaat cagacgaccc
     9901 aacgcccaat attgtttcac acagcctccg cccaacgctt caatcagtca tagacaaaac
     9961 ggcaatccat acgatacaga tcatagcaac agccatcgca acagcgttgg caaaatcagg
    10021 ggactccgac ataggcgcat agcatctgcc gatgcacggc tcctcattcg gctctatgaa
    10081 taccataccc atcacaaaat ccaccgccaa aaccaggcac ggcttcttat acttatgata
    10141 gatttccacc atcctgctcc atatatccca aacattcata ccgtatatcc cgcagacaac
    10201 aaattccgat tgaaggttac agccctat
//
LOCUS       DAWFHY010000071        10180 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.071, whole genome shotgun sequence.
ACCESSION   DAWFHY010000071 DAWFHY010000000
VERSION     DAWFHY010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10180
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.071"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            279..611
                     /locus_tag="WGQ68_001586"
                     /pseudo
     CDS             279..611
                     /locus_tag="WGQ68_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003696591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
     gene            complement(830..1624)
                     /locus_tag="WGQ68_001587"
     CDS             complement(830..1624)
                     /locus_tag="WGQ68_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEZ0715359.1"
                     /translation="MLHRLQKVVRHIAQTEIMPRFLNTPSCRKEDGSMLSEADIAAQT
                     AFAAALPLLIDSPMLGEEMSRQEQSALWEQYSGEKGLWIVDPIDGTNNFVNGLPHFAV
                     SVAFVRNGRAELGVIYNPVSGECFYAERGQGAFLNGTRLPLRLVDKKLNEAIAGVEIK
                     YLRSGKLSSRMSTLAPFGTIRSMGSSTLDWCYLACGRYDVYVHGGQKLWDYAAGALIF
                     EEAGGRLTTLEGDGFWSGEHVFKRSVVAALEPKLFERWVGWIRENQ"
     gene            1728..2453
                     /locus_tag="WGQ68_001588"
     CDS             1728..2453
                     /locus_tag="WGQ68_001588"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEZ0715360.1"
                     /translation="MPRFYLPENLSVGQTVALPDNIVRHLNVLRVRPNENITLFNGKG
                     KAHAAQLTVLEKRRAEAEILHEDTTDNESPLNITLIQSISSGERMDFTLQKSVELGVT
                     AIQPVISERCIVRLDGERAAKRLARWQEIVISACEQSGRNTVPPVLPIIGYREALDKM
                     PSESTKLIMSINRARKLGDIRHPSGAIVFMVGPEGGWTEQEEQQAFEAGFQAVTLGKR
                     ILRTETAPLAALAAMQTLWGDFA"
     gene            complement(3663..4505)
                     /locus_tag="WGQ68_001589"
     CDS             complement(3663..4505)
                     /locus_tag="WGQ68_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0715361.1"
                     /translation="MQNHVISLASAAERRAHIADTFGRHDIPFQFFDALMPSEELNRM
                     MAELVPGLAKQHLLSEVEKACFMSHVVLWKQALDEGVPYVAVFEDDVLLGEGAEKFLA
                     EDGWLKERFDPDSAFIVRLETMFAKVIVRPDKVLNYENRSFPLLESEHWGTAGYIISR
                     EAMRFFLERFAVLPAEWIKAVDWMMFTYFFDKEGMPVYQVNPALCTQELHYAKFLSKN
                     SMLGSDLEKDREQERRHRRSLKVMFDLKRALGKFGREKKKRMERQRQAELEKAYGRRV
                     ISFK"
     gene            complement(4547..4642)
                     /locus_tag="WGQ68_001590"
     CDS             complement(4547..4642)
                     /locus_tag="WGQ68_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lacto-N-neotetraose biosynthesis glycosyl
                     transferase"
                     /protein_id="HEZ0715362.1"
                     /translation="MDIVFAADGRMRRLFTLRQYFGILRRLLKNR"
     gene            complement(4654..5481)
                     /locus_tag="WGQ68_001591"
     CDS             complement(4654..5481)
                     /locus_tag="WGQ68_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 25 protein"
                     /protein_id="HEZ0715363.1"
                     /translation="MQNHVISLASAAERRAHIAATFGARGIPFQFFDALMPSERLEQA
                     MAELVPGLSAHPYLSGVEKACFMSHAVLWKQALDEGLPYIAVFEDDVLLGEGAEKFLA
                     EDAWLKERFDPDSAFIVRLETMFMHVLTSPSGVADYCGRAFPLLESEHWGTAGYIISR
                     KAMRFFLDRFAALPPEGLHPVDLMMFSDFFDREGVPVCQLNPALCAQELHYAKFHDQN
                     SALGSLIEHDRLLNRKQQWRDSPANTFKRRLIRALTKISREREKRRQRREQFIVPFQ"
     gene            complement(5481..6569)
                     /locus_tag="WGQ68_001592"
     CDS             complement(5481..6569)
                     /locus_tag="WGQ68_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002259429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEZ0715364.1"
                     /translation="MPSEAFRRHRAYRENKLQPLVSVLICAYNVEKYFAQSLAAVVNQ
                     TWCNLDILIVDDGSTDGTLAIAQRFQEQDGRIKILAQAQNSGLIPSLNIGLDELAKSG
                     MGEYIARTDADDIAAPDWIEKIVGEMEKDRSIIAMGAWLEVLSEEKDGNRLARHHEHG
                     KIWKKPIRHEDIADFFPFGNPIHNNTMIMRRSVIDGGLRYNTERDWAEDYQFWYDVSK
                     LGRLAYYPEALVKYRLHANQVSSKYSIRQHEIAQGIQKTARNDFLQSMGFKTRFDSLE
                     YRQIKAVAYELLEKHLPEEDFERARRFLYQCFKRTDTPPAGVWLDFAADGRMRRLFTL
                     RQYFGILHRLIKNRRQARSDSAGKEQEI"
     gene            complement(6586..8649)
                     /locus_tag="WGQ68_001593"
     CDS             complement(6586..8649)
                     /locus_tag="WGQ68_001593"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEZ0715365.1"
                     /translation="MMTQTLLIELLTEELPPKALNNLGNHFAAAVAEGLEKAQLVDGA
                     AEFTAYASPRRLAVQVKNVKAVQADQKIVKKGPAVANAVKDGAPTKALEGFARGAGAK
                     IEDLTIVHDGKQDVYAYEYVQTGKPLGGLLEDIINQVVKKLPIPKVMRWGSSTFTFVR
                     PVHGLIVLHGGDIVNVSVLGLQSSNQTLGHRFLSNGEITIENADSYAEQMRGQGKVVA
                     SFAERKAAIQTALEGQARSLNAAVAADEALLDEVTALVEWPVVLEAGFEEHFLAVPQE
                     CLILTMQQNQKYFPLLDQNGKLMNRFLLVSNLQTEDPSHIIRGNERVLRARLSDAEFF
                     YKQDQKATLESRLPKLANVVYHNKIGSQAERIERLQSIAAHIAKALGADAAAAERAAR
                     LAKADLVTEMVGEFPELQGTMGKYYARLDGETEEIAEAIEQHYQPRFAGDKLPESKIA
                     AAVALADKLETLVGIWGIGLIPTGDKDPYALRRAALGILRMLMQYGLDVNELIQTAFD
                     SFPKGLLNEQTPSETADFMQARLAVLLQNDYPQDIVAAVLAKQPRRLDDVVAKLQAVA
                     AFKQLPEAAALAAANKRVQNLLKKADAELGAVNESLLQQDEEKALFAAAQGLQPKIAA
                     AVAEGNFQTALSELASVKPQVDAFFDGVMVMADDAAVKQNRLNLLNRLAEQMNAVADI
                     ALLGE"
     gene            complement(8670..9080)
                     /locus_tag="WGQ68_001594"
     CDS             complement(8670..9080)
                     /locus_tag="WGQ68_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HEZ0715366.1"
                     /translation="MPVYFRTPSRKRLHKMNRRQHKKLHLGEFQALIFGVRGSLHQAA
                     DDAEAFDRFMDELIAYLEANGLEMAGGGDCGDFSCLFQKSGARSLGETEKKAIISWLA
                     GREDVATLYATPLVDGFYGDDGWENYSETYKQAD"
     gene            complement(9156..10061)
                     /gene="glyQ"
                     /locus_tag="WGQ68_001595"
     CDS             complement(9156..10061)
                     /gene="glyQ"
                     /locus_tag="WGQ68_001595"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEZ0715367.1"
                     /translation="MLTFQQIIFKLQTFWADKGCTVIQPFDMEVGAGTSHPATCLRAL
                     GPEPWFAAYVQPSRRPKDGRYGDNPNRLQHYYQFQVALKPAPANIQDLYLDSLRELGI
                     DPKVHDIRFVEDDWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGIDCTPVLGEITY
                     GIERLAMYLQGVENVYDLVWAKTPDGNTVSYGDVYHQNEVEQSTYNFEYSDADWLLRQ
                     FNDYEAQAKRLLAEENAALALPAYELVLKAGHTFNLLDARGAISVTERATYIGRIRAL
                     SRAVAQKYVESREKLGFPLMKANAA"
BASE COUNT         2346 a         2786 c         2585 g         2463 t
ORIGIN      
        1 gagggaaagg aattttgcaa aggtctcggg ctatgccggc tgctgccgcc cattgcacct
       61 tcgccgaatc ctgccgccca ccgccgaagc cctcatgcgt tcgcggtaca gcgcgtatgt
      121 gctactatag tggattaaca aaaaccagta cagcgttgcc tcgccttagc tcaaagagaa
      181 cgattctcta aggtgctgaa gcaccgagtg aatcggttcc gtactatttg tactgtcttc
      241 ggcttcgtcg ccttgtcctg atttttgtta atccactata catcatcgct actaccgttc
      301 cggcgcaaca aacattcctc gatgccgccg aactgatgca atggagtata gaaaccgaag
      361 ggctgggctt gaacgtcatc tcgcacaaga tactcggcaa agaccacgcc caagtcgaat
      421 ttgaagccta cttccgagac ggacaacacc gatccgcgca tcacgaactg tccggcttcg
      481 tcaacatcgg cggacaatgg tattttatcg atcccaccgt tccgcatcct gcgatgaaac
      541 aaccctgcat ttgcggatcg ggcaaaaaat tcaaagcctg ctgcggcaaa tatctgaaac
      601 ctgtcgcata aaaatgccgt ctgaacgttc agacggcatt ttcaacgtgc aaaaaaaaac
      661 cattcatacc aagggtaagt atgaatggtc aatacattgc gggaaaacgt cttacttgct
      721 gcactgccga aaagggagaa acggcagcgg taatcagcgg aaatgattgt acccgaatta
      781 atgttaagaa acgttaatcg cgaaaatata ttaacaaacc tgttgaaacc tattggtttt
      841 cccgtatcca cccgacccag cgttcaaaca gcttcggttc gagcgcggca acgaccgagc
      901 gtttgaacac gtgttcacca ctccaaaacc cgtcgccttc caaagtcgtc agcctgccgc
      961 ccgcctcctc gaaaatcaac gcacccgcag catagtccca cagcttctgc ccaccgtgaa
     1021 cataaacatc ataacgcccg cacgccagat aacaccaatc caacgtactg ctgcccatac
     1081 tccgtatcgt tccaaaaggc gcgagcgtac tcatacggct ggaaagtttg cccgaacgca
     1141 aatatttgat ttccacgccc gcaatcgcct cattgagttt tttatccacg agacgcaaag
     1201 gcaggcgggt tccattaaga aacgcaccct gcccccgttc ggcataaaaa cattcgccgc
     1261 tgaccggatt gtaaatcaca cccaattcgg cgcgcccgtt gcggacaaac gccaccgaca
     1321 cggcaaaatg cggcagcccg ttgacaaaat tgttcgtccc gtctatcgga tcgacaatcc
     1381 acagcccctt ttcccccgaa tattgttccc acaaagccga ctgttcctgc cgcgacattt
     1441 cctcgcccaa catcggactg tcgattaaaa gcggcaacgc ggcggcaaaa gccgtctgcg
     1501 cggcaatgtc cgcctcgctc aacatcgaac cgtcttcctt gcggcaagac ggcgtattca
     1561 aaaaacgcgg cataatttcg gtttgcgcga tatggcgcac gactttctgc aaacggtgca
     1621 acacttccta ctgtcctcat attttgaact tgcggcgcgc aaacgtataa tgtccgtttc
     1681 catcacgccg ctgcgacgga ttataaccgt ccgaaccgcc aaaaactatg ccccgattct
     1741 acctgcccga aaacctttcc gtcggacaaa ccgtcgccct gcccgacaac atcgtccgcc
     1801 acctcaacgt cctgcgcgtc cgccccaacg aaaacatcac cctcttcaac ggcaaaggca
     1861 aggcacacgc cgcacagctg accgttttgg aaaaacgccg cgccgaagcc gaaatcctgc
     1921 acgaagacac aaccgacaac gagtccccgc tcaacatcac gctcatccaa tccatatctt
     1981 ccggcgaacg tatggacttt accctgcaaa aaagcgtcga actcggcgta accgccatac
     2041 agcccgtcat cagcgaacgc tgcatcgtcc gcctcgacgg agaacgcgcc gccaaacgcc
     2101 tcgcacgctg gcaggaaatc gtcatctccg cgtgcgaaca aagcggcagg aacaccgttc
     2161 cccccgtgct gcccatcatc ggctatcgtg aagcactcga caaaatgccg tctgaaagca
     2221 ccaagctgat tatgagcatc aaccgcgccc gcaaactcgg cgacatccgc cacccgtccg
     2281 gcgcaatcgt ctttatggtc gggcccgaag gcggctggac agaacaggaa gaacaacagg
     2341 catttgaagc cggctttcag gcggttacac tcggcaaacg gattttacgc acagaaaccg
     2401 ccccactcgc cgccctcgcc gccatgcaga cgctttgggg cgatttcgca taaacagaaa
     2461 tgccgtctga aacccgttca gacggcattt tgcagccgat taagatagta ggttcaaata
     2521 agatttcccc cgccgtcatt cccgcgaaag caagaatcta gaaacgaaaa actacaggga
     2581 tttatccgaa acaacaaacc ctctccgccg tcattcccgc gcaggcggga atctagaaat
     2641 ttaacgttgc ggtgatttat cggaaatgac tgaaactcaa cggactggat tcccgcctgc
     2701 gcgggaatga cgaattttag gttgctgttt tttggttttc tgtttttgcg ggaatgacga
     2761 attttaggtt tctgtttttg gttttctgtt ctcgcgggaa taacggaatt ttaagtttta
     2821 ggaatttgtc ggaaaaacag aaatcccgcc gccgtcattc ccgcgaaagc gggaatctag
     2881 aaacgaaaaa ctacagggat ttatccgaaa caacaaaccc tctccgccgt cattcccgca
     2941 aaagcgggaa tctggaaatt taatgttgcg gtgatttatc ggaaatgact gaacctcaac
     3001 ggactggatt cccgcctgcg cgggaatgac gaagtggaag ttgcccgaaa cttaaaacaa
     3061 gcgaaaccga acggactgga ttcccgcctg cgcgggaatg acgaatttta ggttgctgtt
     3121 ttttggtttt ctgtttttgc gggaatgacg gaattttaag ttttaggaat ttgtcggaaa
     3181 aacagaaatc ccccgccgtc attcccgcaa aagcgggaat ctagaaattt aacgttgcgg
     3241 tgatttatcg gaaatgactg aacctcaacg gactggattc ccgcctgcgc gggaatgacg
     3301 aagtggaagt tacccgaaac ccaaaacaag cgaaaccgaa cggactggat tcccgcctgc
     3361 gcgggaatga cgaattttag gttgctgttt tttggttttc tgtttttgcg ggaatgacga
     3421 gattttaggt ttctattttt ggttttctgt tttcgcggga ataacggaac tttaagtttt
     3481 aggaatttgt cggaaaaaca gaaatccccc ccccgccgtc attcccgcaa aagcgggaat
     3541 ctagaaattt aacgttgcgg tgatttatcg gaaataactg aaactcaacg gactggattc
     3601 ccgcctgcgc gggaatgaca gtgtgtccat ttctaatttt aatccgctat attttacaca
     3661 aactatttga acgatatgac ccgcctgccg taagctttct caagctccgc ctgcctttga
     3721 cgctccattc ttttcttctt ttctctaccg aatttaccca aagcacgctt caagtcaaac
     3781 attaccttca acgaacggcg gtgtcttctt tcttgttccc tatctttttc caaatcgcta
     3841 cccaacatac tgtttttact gaggaacttg gcataatgca attcttgggt acataaggcg
     3901 ggattaacct gataaacagg catcccctcc ttatcaaaga aataagtaaa catcatccaa
     3961 tctaccgctt taatccactc tgccggcaaa acggcaaacc tttccaagaa aaaccgcatc
     4021 gcctcacgcg aaatgatata gccagccgtc ccccaatgtt cgctctccag caaaggaaat
     4081 gaccggttct cataattcag gactttatcc ggtctgacaa taactttcgc aaacatcgtt
     4141 tccaaacgga cgataaaggc ggaatccggg tcaaaccgct cttttagcca gccgtcttcg
     4201 gcaaggaatt tctccgcccc ttcgccgagt aagacatcat cctcaaatac ggcaacatac
     4261 ggtacgcctt cgtccaatgc ctgtttccac aatacgacgt ggctcataaa gcaggctttt
     4321 tccacttcgc tcaacaggtg ctgttttgcc aatcccggca ccaattccgc catcatccga
     4381 ttcagttctt cagacggcat cagtgcgtcg aaaaactgaa acggaatgtc gtgcctgccg
     4441 aaggtatcgg caatgtgcgc cctgcgttct gcggcggaag ctaagctgat aacatggttt
     4501 tgcataattt atcctgtttt ttgtctgttg gataaagcgg cgtttttcaa cggtttttca
     4561 gcaatcggcg caaaatgccg aagtattgcc tcaaggtaaa cagccgccgc atcctgccgt
     4621 ctgctgcaaa tacgatgtcc atctctcctc cttttattgg aaaggcacaa tgaactgttc
     4681 gcgcctttgc cggcgttttt ccctttccct gctgattttg gtcaaggcgc ggatcaggcg
     4741 gcgtttgaat gtgttggcgg gggaatcgcg ccattgctgt ttgcggttca ggaggcggtc
     4801 gtgttcgatc aggctgccca atgcgctgtt ttggtcgtga aacttggcat aatgcagctc
     4861 ttgggcgcac aaggcgggat tgagctggca aaccggcact ccttccctgt cgaaaaaatc
     4921 gctgaacatc atcagatcga cggggtgcag cccttcgggc ggcagggcgg caaacctgtc
     4981 caggaaaaac cgcatcgctt ttcgggaaat gatatagccc gccgtccccc agtgttcgct
     5041 ttccaacagc ggaaaggcac gcccgcagta gtccgccaca ccggagggcg aggtcaggac
     5101 gtgcataaac atcgtttcca agcggacgat aaaggcggaa tcggggtcaa agcgttcttt
     5161 tagccaagcg tcttcggcaa ggaatttctc cgcgccttcg ccgagtaaaa cgtcgtcctc
     5221 aaatacggcg atatacggca gaccttcgtc caatgcctgt ttccacaata cggcgtggct
     5281 cataaagcag gctttttcca ctccgctcaa ataggggtgc gccgacaagc cggggacgag
     5341 ttccgccatt gcctgttcca gcctttcaga cggcatcagt gcgtcgaaaa actgaaacgg
     5401 gatgccgcgc gcgccgaagg ttgcggcaat gtgcgccctg cgttctgcgg cggaagctaa
     5461 gctgataacg tggttttgca ttaaatctcc tgttctttcc ctgccgaatc cgaccgcgcc
     5521 tgccggcggt ttttaatcag ccggtgcaaa atgccgaagt attgcctcaa ggtaaacagc
     5581 cgccgcatcc tgccgtctgc cgcaaaatcc agccacacgc cggcgggcgg cgtgtccgtc
     5641 cgtttgaagc attggtacaa aaaccggcgg gcgcgttcaa aatcttcttc cggcaaatgt
     5701 ttctccagca attcatacgc tactgctttt atttggcggt attcaaggct gtcgaaccgg
     5761 gttttaaaac ccatagactg caaaaaatcg tttctggcgg ttttttggat gccttgcgcg
     5821 atttcgtgtt ggcggatgct gtatttggat gaaacctgat tggcgtgaag gcggtatttg
     5881 accaaggctt cgggataata agccagcctg cccaatttgc tgacatcgta ccaaaattgg
     5941 taatcttccg cccaatcccg ctcggtgttg taacgcaaac cgccgtcaat gacgctgcgc
     6001 ctcataatca tcgtgttgtt gtgtatgggg ttgccgaaag ggaaaaagtc ggcaatatct
     6061 tcgtgccgga tcggcttttt ccaaattttg ccgtgttcgt ggtgccgcgc cagccggttg
     6121 ccgtcctttt cttccgacaa aacttccagc cacgcgccca tcgcaatgat gctgcggtct
     6181 ttttccatct cgcccacgat tttctcaatc caatcggggg cggcaatatc gtctgcatcg
     6241 gtgcgtgcaa tatattcccc cattcctgac tttgccaatt cgtccagccc gatgtttaaa
     6301 gaaggaatca gaccggaatt ttgagcttgt gcaaggattt tgatgcggcc gtcctgttct
     6361 tggaaacgct gggcaatggc aagcgtaccg tccgtcgagc cgtcatcgac aatcaaaata
     6421 tccaagttgc accaagtttg attcacgacg gcggctaatg attgggcgaa atatttttct
     6481 acgttgtagg cgcaaatcaa tacgctgact aaaggctgca atttattctc ccgataggca
     6541 cgatgccgtc tgaaggcttc agacggcatt tggactgtac aacggttact cgcccaaaag
     6601 cgcgatgtcg gcaactgcgt tcatttgttc tgccaagcgg ttcaacagat tcaaacggtt
     6661 ttgtttcacg gcggcatcgt ccgccatcac catcacgccg tcaaagaagg catcgacttg
     6721 cggcttgacg gaagccagtt cggacaaggc ggtttggaaa ttgccttcgg caacggcggc
     6781 ggcgattttc ggctgcaaac cttgcgcggc ggcaaagagg gctttttctt cgtcctgttg
     6841 cagcaggctt tcgttaaccg cgcccaactc ggcatcggct tttttcagca gattttgcac
     6901 gcgtttgttg gcggcggcga gcgcggcagc ttcgggcagt tgtttgaacg cggcaacggc
     6961 ttgcagtttc gccactacgt cgtccaaacg gcgcggctgt ttcgccaata cggcggcaac
     7021 gatgtcttgc ggataatcgt tttgcagcaa cacggcaagg cgcgcctgca taaagtcggc
     7081 ggtttcagac ggcgtttgct cgttgagcaa gcctttgggg aagctgtcga atgccgtctg
     7141 aatcagttcg ttcacgtcca aaccgtactg catcagcatc cgcaaaatac ccaaggcggc
     7201 gcggcgcagg gcgtaggggt ctttatcgcc ggtcggaatc agaccgatgc cccaaatgcc
     7261 gaccagggtt tccagcttgt ccgccagtgc cacggcggcg gcaattttgc tttcgggcag
     7321 cttgtcgccg gcaaaacgcg gctgatagtg ctgctcgatg gcttcggcga tttcttcggt
     7381 ttcgccgtcc aaacgggcat agtatttgcc catggtgcct tgcaattcag ggaactcgcc
     7441 gaccatttcg gttaccaagt cggcttttgc cagacgcgcg gcgcgttcgg cggcggcggc
     7501 atctgcgccc aacgctttag cgatgtgggc ggcaatgctt tgcaggcgtt cgatgcgttc
     7561 ggcttgcgaa ccgattttgt tgtgataaac cacgtttgcc agcttgggca ggcggctttc
     7621 caaagtcgcc ttttggtctt gtttgtagaa gaactcggca tcagacaggc gcgcgcgcaa
     7681 aacgcgttcg ttgcctcgga tgatgtgcga cgggtcttcg gtttgcaggt tggaaaccag
     7741 caggaagcgg ttcatcagct tgccgttttg gtcgagcagc ggaaagtatt tttggttttg
     7801 ctgcatcgtt aaaatcaagc attcctgcgg cacggcgagg aaatgttcct caaaaccggc
     7861 ttccaaaaca acaggccatt cgaccagcgc ggttacttcg tccaacaatg cttcatcggc
     7921 ggcgacggcg gcgttcagac tgcgcgcctg cccttccaat gccgtctgaa tcgcggcttt
     7981 gcgctcggca aacgaagcga cgactttgcc ttgcccgcgc atttgttcgg cgtagctgtc
     8041 ggcgttttca atggtgattt cgccgttgga caagaagcgg tgtcccaagg tttgattgct
     8101 gctttgcaga cccaaaacgc tgacgtttac gatgtcgccg ccgtgcagca caatcagccc
     8161 gtgaacaggg cgcacgaagg taaatgtgct gctgccccaa cgcatcactt tgggaatcgg
     8221 cagtttctta accacttgat tgataatgtc ttccaaaagt ccgcccagcg gcttgccggt
     8281 ttggacgtat tcgtaggcgt acacatcctg cttgccgtcg tggacgatgg tcaagtcttc
     8341 gattttcgcg cctgcaccgc gtgcgaaacc ttccaaagcc ttggtcggcg caccgtcttt
     8401 tacggcattc gccacggcag ggcctttttt cacgattttt tggtcggcct gaacggcttt
     8461 gacgtttttg acttgaaccg ccaaacggcg cggcgaggca taagccgtaa attcggctgc
     8521 accgtcaacc agttgcgctt tttccaagcc ttcggcaacg gcggcggcaa aatggttgcc
     8581 cagattattc agggctttgg gcgggagttc ttcggtaagg agttcgatta aaagggtttg
     8641 ggtcatcatt cggctttctt tgaatttggt taatctgcct gtttataggt ttcgctgtaa
     8701 ttttcccagc cgtcatcccc gtaaaaacca tcaaccagcg gggtggcgta caaagtggcg
     8761 acatcttcgc ggcctgccag ccaagagata atggcttttt tctcggtttc gcccaagctt
     8821 cgggcaccgg atttttgaaa caggcacgaa aaatcgccgc aatcgccccc gcccgccatt
     8881 tccaagccat ttgcctcaag atacgcaatc agctcgtcca taaagcggtc gaacgcttca
     8941 gcatcgtccg cagcttggtg caaactgcct cgaacgccga aaatcaatgc ttgaaactcg
     9001 cccaaatgca gctttttatg ctggcggcgg ttcattttgt gcaggcgttt cctgctcggg
     9061 gtgcggaaat agacaggcat aattttccta aaaaatataa tggcttccgg acggctgcca
     9121 tatcgtgccg cccgaacgta aaaatcgtcg ctcccctagg cggcatttgc cttcattaag
     9181 ggaaagccca gtttttcgcg gctttcgaca tatttttgcg ccacggcgcg gctcaacgcg
     9241 cggatgcgcc cgatgtaggt ggcgcgttcg gttacggaaa tcgcgccgcg cgcgtccaag
     9301 aggttgaacg tatgccccgc tttgaggacc agctcgtagg caggcagggc gagggcggcg
     9361 ttttcttcgg caagcaggcg tttggcttgc gcttcgtagt cgttgaactg gcgcagcagc
     9421 caatcggcat cgctgtattc gaagttgtag gtggattgtt cgacttcgtt ttggtggtac
     9481 acgtcgccgt agcttacggt gttgccgtcc ggcgtttttg cccaaacgag gtcatagacg
     9541 ttttccacgc cttgcaggta catcgccaag cgttcgatgc cgtaggtaat ttcgccgagt
     9601 acaggcgtgc agtcaatgcc gccgacttgt tggaaatagg taaactgggt tacttccatg
     9661 ccgttgagcc agacttccca gcccaaaccc catgcaccga gggtggggtt ttcccagtcg
     9721 tcttcgacaa agcggatgtc gtggactttg gggtcgatgc ccaattcgcg cagggagtcg
     9781 agatagaggt cttggatatt ggcgggagcg ggcttgaggg cgacttggaa ttggtaatag
     9841 tgttgcaggc ggttggggtt gtcgccgtag cgaccgtctt tggggcggcg gctgggttgg
     9901 acgtaggcgg caaaccaagg ctcgggcccg agtgcgcgca ggcaggtggc ggggtgggaa
     9961 gtacctgcac cgacttccat gtcgaagggt tggataacgg tgcagccttt gtctgcccag
    10021 aatgtttgca gtttgaagat gatttgttgg aaggtaagca tggcttatga ttcgataaaa
    10081 taaagggttt attttactgt ttccattgct gtttggatag gtttatctca aagacagact
    10141 gatttgaaaa cacggcatac atgatatagt ggattaaatt
//
LOCUS       DAWFHY010000072        10088 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.072, whole genome shotgun sequence.
ACCESSION   DAWFHY010000072 DAWFHY010000000
VERSION     DAWFHY010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 10088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10088
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.072"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..111
                     /locus_tag="WGQ68_001596"
     CDS             <1..111
                     /locus_tag="WGQ68_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0715368.1"
                     /translation="RAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
     gene            904..1722
                     /gene="opc"
                     /locus_tag="WGQ68_001597"
                     /gene_synonym="opcA"
     CDS             904..1722
                     /gene="opc"
                     /locus_tag="WGQ68_001597"
                     /gene_synonym="opcA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Opc"
                     /protein_id="HEZ0715369.1"
                     /translation="MKKTVFTCAMIALTGTAAAAQELQTANEFTVHTDLSSISSTRAF
                     LKEKHKAAKHISVRADIPFDANQGIRLEAGFGRSKKNIINLETDENKLGKTKNVKLPT
                     GVPENRIDLYTGYTYTQTLSDSLNFRVGAGLGFESSKDSIKTTKHTLHSSRQSWSAKV
                     HADLLSQLGNGWYINPWSEVKFDLNSRYKLNTGVTSLKKDINQKTNGWGFGLGANIGK
                     KLGESASIEAGPFYKQRTYKESGEFSVTTKSGDVSLTIPKTSIREYGLRVGIKF"
     gene            1885..2529
                     /locus_tag="WGQ68_001598"
     CDS             1885..2529
                     /locus_tag="WGQ68_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEZ0715370.1"
                     /translation="MLASAIDFILHIDQHLLALSAQYGVWIYAILFLIVFCETGLIVT
                     PLLPGDSLLFAAGGIAALGGMDIHLMVALLSLAAILGDALNFTVGKYFGGRLFANPDS
                     KIFRREYLDKTHRFYEKHGGKTIIIARFMPIVRTFAPFVAGMGKMHYAKFIRYNIIGG
                     LLWVILFSYAGYFFANFPVVKNNLGLVMGGIIIVSVLPGAVEIARAKLAVKSKR"
     gene            2548..4458
                     /locus_tag="WGQ68_001599"
     CDS             2548..4458
                     /locus_tag="WGQ68_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEZ0715371.1"
                     /translation="MNILSVENASFAIGHVALLDKTSFQLDSGEKVGLIGRNGAGKSS
                     FLKILAGVQKLDDGQIIVQNNLKIVYVPQESFFDKDATVFDTVAEGLGEIRDLLRRYH
                     HVSHELENGSSEALLKELNELQLEIEAKDGWKLDAAVKQTLGELSLPENEKIGNLSGG
                     QKKRVALAQAWVQKPDVLLLDEPTNHLDIDAIIWLENLLKAFEGSLAVITHDRRFLDN
                     IATRIVELDRGILRSYPGSFSKYSEKKAQELAVEAEHNRLFDKFHAQEEAWIRKGIEA
                     RRTRNEGRVRRLEELRRQRAERRNVQGQVNFKLDSGEKSGKIIAELEHASFAYGGKVI
                     MDKFSAILQRGDKIGLIGPNGIGKTTFLKLILGELQPTYGRIRIGSKQEVAYFDQFRS
                     ALNENDTVFYTLGQGNDYVEVGGKKKHVMSYLEDFLFPPARAQSPVSSLSGGERNRLL
                     LAKLFTRPANILVLDEPTNDLDIDTQELLEDLLRDYQGTVFLVSHDRMFLDNVITQSL
                     VFEGNGRLKEYIGGYQDYIDAKSREAKIQTESAPKTADAEPVKEKPKANRTVKLSYKE
                     QRELDALPDEIAALEAEQAEINAQLSDPEIFKDYEKAGALQSRAEEIEMLLLEKLERW
                     ELLETKQNGNAV"
     gene            4479..4844
                     /locus_tag="WGQ68_001600"
     CDS             4479..4844
                     /locus_tag="WGQ68_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715372.1"
                     /translation="MFKRPEEIIVLILAVLWIAGTYFLAALFGADAYTVLKITALTLL
                     WSAASFLLWQKNLSRHIWPLLLGCLTACWWPYLNQSVAPGSLPWYASWTFKTILALIP
                     VTAGYLFKWKQYRQRKTKP"
     gene            4955..6430
                     /locus_tag="WGQ68_001601"
     CDS             4955..6430
                     /locus_tag="WGQ68_001601"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009173174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEZ0715373.1"
                     /translation="MISKQEYQAQAAQGYNRIPLVQELLADLDTPLSLYLKLANRPYT
                     YLLESVVGGERFGRYSFIGLPCSHYLKASGKHVDVYQNGEIVEQHDGNPLPFIEAFHN
                     RFKTPEIPSLPRFTGGLVGYFGYETIYNFEHFAHRLKNTTKADPLGTPDILLMLSQEL
                     AVVDNLSGKIYLIVYADPSQPDGYERARERLEDIRTQLRQSCAIPLSLGSKHTEAVSE
                     FGEEPFKACVNKIKDYIFAGDCMQVVPSQRMSMEFTDSSLALYRALRTLNPSPYLFYY
                     DFGDFHIVGSSPEILVRRERDDVIVRPIAGTRLRGKTPAEDLANEQDLLSDAKEIAEH
                     VMLIDLGRNDVGRISKTGEVKVTDKMVIEKYSHVMHIVSNVEGRLKDGMTNMDILAAT
                     FPAGTLSGAPKVRAMEIIEEVEPSKRGIYGGAVGVWGFNNDMDLAIAIRTAVVKNNTL
                     YVQSGAGVVADSDPASEWQETQNKARAVIHAAQMVQEGLDK"
     gene            6427..6972
                     /locus_tag="WGQ68_001602"
     CDS             6427..6972
                     /locus_tag="WGQ68_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEZ0715374.1"
                     /translation="MTYEKLYELRQTLRPTTKDGLYTDNEKSREILTVRWSGNTENPK
                     NFSAVAIGINPSKANDERSDKTLTQLARFLDMYGFTNFKMLNIFSSYSTQQTGIRANT
                     QTDFSKFKGCLEDADMIILAWGTDRGAYKDEKNRILEFLKAEKFMEKVFCISETSNSS
                     DTRHPSRISYSYQLVQFEESA"
     gene            6969..8105
                     /locus_tag="WGQ68_001603"
     CDS             6969..8105
                     /locus_tag="WGQ68_001603"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEZ0715375.1"
                     /translation="MKNISLSPPAMLGILGGGQLGRMFTVAAKTMGYKVTVLDPDPDA
                     PAAEFADCHLCAPFNDQAALDELAKCAAVTTEFENVNADAMRFLAKHTNVSPGSDCVA
                     IAQNRIQEKAWIRKAGLQTAPYQAICKAEDITEESIQFLPGILKTATLGYDGKGQIRV
                     KTVDELKAAFAEHRGVDCVLEKMVDLRGEISVIVCRLNNDNVQTFDPAENIHENGILA
                     YSIVPARLSADVQQQARQMAQRLADELDYVGVLAVEMFVVGDTHELVVNEIAPRPHNS
                     GHHTVDACAADQFQQQVRLMCNLPPADTKLLSSCCMANILGDVWQEDGGEPDWFPLQS
                     RPDAHLHLYGKKTAHKGRKMGHFTVLTTDSDTAFQEAKKLHQSL"
     gene            8152..8634
                     /locus_tag="WGQ68_001604"
     CDS             8152..8634
                     /locus_tag="WGQ68_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEZ0715376.1"
                     /translation="MNLLTLLRPATVQDCKDIFKVHLHSVQYTCILSYNEHALKVWEG
                     LLNTESYLPTISDPDKALWVAEYKGNIQGFFQIDCQEAQLDALYVHPLFHNLGLGTAL
                     LHQAETIAHKSGLSFLKLYASLNSVPFYLLNRYESLGSAVLQLDPSIKIKCELMRKHL
                     "
     gene            8740..9795
                     /locus_tag="WGQ68_001605"
     CDS             8740..9795
                     /locus_tag="WGQ68_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0715377.1"
                     /translation="MKTYAPALYTAALLTACSPTADSNHPSGQNAPANTESDGKNITL
                     LNASYDLARDFYKEYNPLFIKTYQSEHPGTSVSIQQSHGGSSKQALSVANGLQADVVT
                     MNQSSDIDLLEKKGLVEKGWQQALPDHAAPYTSTMVFLVRKNNPKQIRDWNDLAKDGV
                     NIVIANPKTSGNGRYAFLGAYGYGLKTTNGNEQEAQKLVASILKNTPVFENGGRAATT
                     TFTQRNIGDVLITFENEANYVSKKLTQGQFEIVYPSYTISAESPVAVVNSIVAKKGTQ
                     KTARAYLEYLWSEPAQELAASLYLRPRNPEVLARHKADFPDLDTFSPEEKFGGWDNIM
                     KTYFADGGIFDRLTAQK"
     gene            9792..9989
                     /locus_tag="WGQ68_001606"
     CDS             9792..9989
                     /locus_tag="WGQ68_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715378.1"
                     /translation="MTAMPSEPPFRRHFTHQNRKTAPKIIITRHNGKPDTKPNTAAKM
                     PSEAVGATILIWGITFLNHPF"
BASE COUNT         2809 a         2816 c         2243 g         2220 t
ORIGIN      
        1 cgggcagcct atttcggact gattaaagtg agtgcgcaaa gccatctgaa ggcgatgtgt
       61 ttgaacctgt tgaaagccgc caacaggcta agtgcgcccg ctgccgccta aaaggcagcc
      121 cggatgcctg attatcgggt atccgggcag gattaagggg gtgtttgggt aaaattaggc
      181 ggtatttggg gcgaaaacag ccgaaaacct gtgtttgggt ttcggctgtc gggagggaaa
      241 ggaattttgc aaaggtctca aacaaaaaca gaaacctaaa gtcccgtcat tcccgcgcag
      301 gcgggaatcc agacccccaa cgcggcagga atctatcgga aataaccgaa accggacgaa
      361 cctagattcc cgctttcgcg ggaatgacgg cagggtggtt tcagttgctc ccgataaatg
      421 ccgccatctc aagtctcgtc attcccttaa aacagaaaac cgaaatcaga aacctaaaat
      481 cccgtcattc ccgcgcaagc gggaatctag gtttgtcggc acggaaactt atcgggtaaa
      541 acggtttctt tagattttac gttctggatt cccgcctgcg cgggaatgac gatgaaaaga
      601 ttgttgtcgc ttcggataaa tttttgccgt gttgggttct agattcccgc tttcgcggga
      661 atgacggcgg cgggtttctg ttttccgata aatacacaca aactaaaatt tcgtcattcc
      721 cataaaaaac agaaaaccaa gtgagaataa caattcgttg taaacaaata actatttgtt
      781 aatttttatt aatatatgta aaatcccccc ccccgaaagc ttaagaatat aattgtaagc
      841 gtaacgatta tttacgttat gttaccatat ccgactacaa tccaaatttt agaggtttta
      901 actatgaaaa aaacagtttt tacatgtgcc atgattgccc tgaccggtac tgccgccgct
      961 gcacaagagc ttcaaaccgc taatgagttt accgtccaca ccgacctctc ttccatttct
     1021 tcaactcgtg ctttcctgaa agaaaaacac aaagctgcca aacacatcag cgtgcgtgct
     1081 gatattcctt ttgatgccaa ccaaggcatc cgcttggaag ccggtttcgg gcgcagcaaa
     1141 aaaaatatta ttaatttgga aacagatgag aacaagctgg gtaagactaa aaatgtaaaa
     1201 ctgcccaccg gcgttcctga aaaccgtatc gatctttaca caggctacac ctacacccaa
     1261 acgttaagtg attctttaaa tttccgtgtg ggtgccggct tgggttttga atcttcaaaa
     1321 gacagcatta aaaccaccaa gcatacgctt cacagcagcc gtcagtcgtg gtcagccaaa
     1381 gttcacgcgg atttgctttc ccaactgggt aacggctggt atatcaaccc ttggtctgaa
     1441 gtgaaatttg acctcaattc ccgctataaa ttaaacaccg gcgttaccag tctcaaaaaa
     1501 gacatcaatc aaaaaaccaa cggctggggc tttggattgg gtgcaaatat tggtaaaaaa
     1561 ctgggcgaat ccgccagcat cgaggcgggg ccgttctaca aacaacgcac ttacaaagaa
     1621 tccggcgagt ttagtgtaac aaccaagagt ggcgacgtat cgctcaccat cccgaaaacc
     1681 agtattcgtg aatacggctt gcgcgtcggc ataaaattct gatgatttga aatcatcatt
     1741 gtcactttaa atgccaaacc gcaaactatt ttggtttgcg gtttttacgt gaaatgaatt
     1801 tgaatagccg atgccgtctg aaacttttca gacggcattt ttccaatcgg ttaaaatacg
     1861 cggtttgttt tttaggaacg cgccgtgctt gcctccgcca tcgacttcat cctccatatc
     1921 gaccaacacc tgctcgcgct gtcggcgcaa tacggtgtgt ggatttatgc gattctgttt
     1981 ttgattgttt tttgcgaaac cggcctgatt gttacgccct tactgcccgg cgattcgctg
     2041 ctgtttgccg ccggcgggat tgccgcgctg ggcggtatgg atattcattt gatggttgca
     2101 ttattaagcc ttgccgccat actcggcgac gcgctcaatt ttaccgtcgg caaatatttc
     2161 ggcggcaggc tgttcgccaa ccccgattct aaaatcttcc gacgtgaata tctcgataaa
     2221 acccaccgtt tttacgaaaa acacggcggc aaaaccatca tcattgcccg ttttatgccc
     2281 atcgtccgca ccttcgcccc cttcgtcgcc ggtatgggga aaatgcacta tgccaaattt
     2341 atccgctaca acatcatcgg tggcttgttg tgggttatcc tgttttctta cgcgggctat
     2401 ttctttgcca acttccccgt cgtaaaaaac aatctgggct tggtgatggg cggcatcatt
     2461 attgtttccg tcctgccggg tgcagttgaa attgcccgcg ccaaacttgc ggtaaaatcc
     2521 aaacggtaac tgttgagaat ttgtatcatg aatatattat ctgtagaaaa cgcttctttt
     2581 gccatcggcc acgttgcctt gctcgacaaa acttcttttc aactcgacag cggcgaaaaa
     2641 gtcggcttga tcggacgcaa cggcgcaggc aagtcttcgt ttttaaaaat cctcgccggt
     2701 gtgcaaaagc tcgacgacgg gcagattatt gttcaaaaca acctcaaaat cgtttatgta
     2761 ccgcaggaat ccttttttga taaggacgca accgtatttg ataccgttgc cgaaggtttg
     2821 ggcgaaattc gtgatttatt gcgccgttat catcatgtca gccatgagtt ggaaaatggt
     2881 tcgagtgagg ctttgttgaa agaactcaac gaattacaac ttgaaatcga agcgaaggat
     2941 ggctggaagc tggatgcggc agtcaagcag actttggggg aactcagttt gccggaaaat
     3001 gaaaaaatcg gcaacctttc cggcggtcag aaaaagcgtg tcgccttggc gcaggcttgg
     3061 gtgcagaagc ccgacgtatt gctgctggac gaaccgacca accatttgga tattgacgcg
     3121 attatctggt tggaaaacct gctcaaggcg tttgaaggca gcttggccgt gattacccac
     3181 gaccgccgtt ttttggataa tatcgctacg cggattgtcg aacttgaccg cggcattcta
     3241 cgttcctatc ccggctcgtt ctctaaatac agtgagaaaa aagcgcaaga gttggcagtc
     3301 gaggcggaac ataaccgctt gtttgacaaa tttcatgctc aggaagaagc gtggatacgc
     3361 aaaggcatcg aagcgcgccg tacccgtaac gaaggacgcg tgcgccgttt ggaagagctg
     3421 cgccgccaac gtgcagaacg ccgcaacgtg cagggacagg tcaactttaa gctcgatagc
     3481 ggtgagaaaa gcggcaaaat catcgccgaa ttggaacacg cttcgtttgc ctatggcggc
     3541 aaagtcatta tggacaaatt ctccgccatc ttgcagcgtg gcgacaaaat cggcttaatc
     3601 ggcccaaacg gtatcggcaa aaccaccttc ctcaagctga ttttgggcga attgcagccg
     3661 acctacggca gaatccgtat cggcagcaag caggaagtcg cctatttcga ccagttccgc
     3721 agcgcgttga atgaaaacga cactgtgttt tacacgctcg gacagggcaa tgattacgtt
     3781 gaagtcggcg gtaagaaaaa gcatgtgatg agctatttgg aagatttcct gttcccccct
     3841 gcccgcgctc aaagtcctgt ttcttcactc tccggcggcg aacgcaaccg tcttttgctg
     3901 gcaaaactct ttacccgtcc cgccaatatt ttggtcttgg acgaaccgac caatgacttg
     3961 gacatcgaca cccaagagct gctcgaagac ctgttgcgcg attatcaagg cacggtattc
     4021 cttgtctcgc acgaccgtat gttcctcgat aacgtgatta cccaaagcct tgtttttgaa
     4081 ggaaatggaa ggttaaaaga atatatcggc ggctatcagg actacatcga cgcaaaatca
     4141 cgcgaagcga agattcagac ggaatccgca cccaaaacag ccgatgccga accggtgaaa
     4201 gaaaaaccca aagccaaccg cacggtcaaa ctgtcctaca aagaacagcg cgaactcgac
     4261 gccctgcccg acgaaatcgc cgccttggaa gccgaacagg cagaaatcaa cgcgcagctt
     4321 tccgatcccg aaatcttcaa agattacgaa aaagccggcg cactgcaaag ccgcgccgaa
     4381 gaaatcgaaa tgctgctttt ggaaaagctg gaacgctggg agctgctgga aaccaaacag
     4441 aacggcaatg ccgtctgaaa tcaaacagga agaaaaaaat gttcaaacga cctgaagaaa
     4501 tcattgtcct gatacttgcc gtcctctgga ttgccggtac gtattttctc gccgccctgt
     4561 ttggagcgga tgcttatacc gtgttaaaaa tcactgccct taccctgctt tggtctgccg
     4621 catcattcct cctttggcaa aaaaacctca gccggcatat ctggccgctg ctgctcggct
     4681 gcctgactgc ctgttggtgg ccgtatctga atcaatcggt cgcacccggt tctttacctt
     4741 ggtatgcatc atggacgttc aaaaccatct tagccctgat tcccgtaacc gcaggttatc
     4801 tgtttaaatg gaaacaatac cggcagcgca aaacaaagcc gtaagcccgt ccttttcaga
     4861 cggcatctcc ggtcagactg tcaacaattc cgataaaatg ctatactcgc gcccgttgcc
     4921 gcgccgtccg aaaccgccct ttgggaaagc agaaatgatc agcaaacaag aataccaagc
     4981 ccaagccgct caaggctaca accgcatccc gctcgtgcaa gaactccttg ccgatttgga
     5041 tacgccgctt tccctctatc tcaaactcgc caaccgcccc tatacctacc tgctcgaatc
     5101 cgttgtcggt ggcgaacgtt tcggccgcta ttcttttatc ggcctgcctt gcagccacta
     5161 tctcaaagcc agcggcaaac acgtcgatgt ttatcaaaac ggcgaaattg tcgagcaaca
     5221 cgacggcaac cccctgccct ttatcgaagc cttccacaac cgcttcaaaa cacccgaaat
     5281 cccaagcctg ccgcgcttta ccggcggact ggtcggctac ttcggttacg aaaccatcta
     5341 caattttgaa cacttcgccc accgcctgaa aaacaccacc aaagccgacc cgctcggcac
     5401 gcccgacatc ctcctgatgc tctcgcaaga gcttgcagta gtggataacc taagcggtaa
     5461 aatctacctg attgtttacg ccgatccgtc gcagcccgac ggctacgaac gcgcacgcga
     5521 acgcctcgaa gacatccgta cacagttacg ccaaagttgc gccatcccgc tttcgctcgg
     5581 cagcaaacac accgaagccg tcagcgaatt cggcgaagag ccgttcaaag cctgcgttaa
     5641 caaaatcaaa gactacatct ttgcaggcga ctgcatgcag gttgtcccca gccagcgcat
     5701 gagcatggaa ttcaccgaca gctcgctcgc cctctaccgc gccttgcgca cgctcaaccc
     5761 ttcgccttat ctcttctact acgatttcgg cgatttccac atcgtcggct cctcgcccga
     5821 aatcctcgtc cgccgcgaac gcgacgatgt catcgtccgc cccatcgccg gcacgcgcct
     5881 gcgcggcaaa acccccgccg aagaccttgc caacgaacaa gatttgttaa gcgatgccaa
     5941 agaaatcgcc gaacacgtca tgttgattga cttaggacgc aacgacgtcg gccgcatcag
     6001 taaaaccggc gaagtcaaag ttaccgacaa aatggtgatt gaaaaatact cccatgtaat
     6061 gcacatcgtt tccaacgtcg aaggccgtct gaaagacggc atgaccaaca tggacatcct
     6121 cgccgctacc ttccctgccg gcacactctc cggcgcaccc aaagtccgcg ccatggaaat
     6181 catcgaagaa gtcgaaccaa gcaaacgcgg catctacggc ggtgccgtcg gcgtatgggg
     6241 tttcaacaac gacatggatt tggcaatcgc catccgcacc gccgtagtaa aaaacaacac
     6301 gctatacgtc caaagcggcg cgggcgtggt tgccgactcc gatccggcct ccgaatggca
     6361 ggaaacacaa aacaaagccc gagccgtcat acacgccgcg cagatggtgc aggaagggtt
     6421 ggataaatga cttatgaaaa actttatgag cttcgccaaa cactcagacc gactaccaaa
     6481 gacggtctct atacagataa tgaaaaaagt cgagaaatcc taacggttag atggagcggc
     6541 aacacagaaa atcctaaaaa tttctcagct gttgctatag gaattaatcc aagtaaagca
     6601 aatgatgaaa gaagcgacaa aacccttacc caacttgcac gtttcttgga tatgtacggt
     6661 tttaccaact tcaaaatgtt aaatattttt tccagttact ctacacagca aacaggaatt
     6721 agggcaaaca cccaaaccga tttttccaaa tttaaaggat gtttggaaga tgctgatatg
     6781 attattcttg catggggtac agaccgcggt gcttataagg atgaaaaaaa cagaatactg
     6841 gaatttctaa aagcagaaaa atttatggaa aaagtattct gtatttctga aactagtaat
     6901 tccagcgata caagacatcc gtcaagaatt agttatagct atcaacttgt acaatttgaa
     6961 gagtcagcat gaaaaacata tctctttctc cgcccgccat gcttggcatc ctcggcggcg
     7021 gacaattagg cagaatgttt accgttgccg ccaaaaccat gggctacaaa gtaaccgttc
     7081 tcgaccccga tccggacgcg ccggcagcag aatttgccga ctgtcattta tgcgcgccgt
     7141 ttaacgacca agctgctttg gacgaattgg caaaatgcgc ggcggtgacc actgaatttg
     7201 aaaacgtcaa tgccgatgcg atgcgttttc tcgccaaaca caccaatgtt tcccccggca
     7261 gcgactgcgt tgccatcgcg caaaaccgca ttcaggaaaa ggcatggata cgcaaagcag
     7321 gattgcaaac cgcgccgtat caagcaattt gcaaagccga agacatcact gaagaaagca
     7381 tacaatttct gcccggcatc ctgaaaaccg ctacattggg ctatgacggc aaaggccaaa
     7441 tccgcgtcaa aacggtggat gaactcaaag ccgcgtttgc cgaacaccgc ggcgtggatt
     7501 gcgttttgga aaaaatggtg gacttgcgcg gcgaaatttc cgttatcgta tgccgtctga
     7561 acaatgacaa cgtgcaaact ttcgatcctg ccgaaaacat tcacgaaaac ggtatcttgg
     7621 cttattccat cgtccccgcg cgactgagtg ccgacgtgca gcaacaggcg cggcagatgg
     7681 cgcaacgctt ggcagacgaa ttggattatg tcggcgtatt ggcggtagaa atgtttgttg
     7741 tcggtgacac acatgaattg gtcgtcaacg aaatcgcgcc gcgtccgcac aactccggcc
     7801 accataccgt cgatgcctgc gcggcagacc aattccagca acaggtccgc ctgatgtgca
     7861 acctgccacc tgctgacacc aaattgctga gttcttgctg tatggcgaat attttgggcg
     7921 acgtttggca ggaagacggc ggcgaaccgg attggtttcc cctgcaaagc cggccggacg
     7981 cgcacctgca cctttacggc aaaaaaaccg cgcacaaagg gcggaaaatg ggacacttta
     8041 ccgttttaac caccgattcg gacaccgcat ttcaagaagc aaaaaaactg catcagtccc
     8101 tataatccga ccaacggcac agatgccgtc tgaagccgcc gacatactgc catgaacctg
     8161 ctgacactgt tacgccccgc caccgtgcaa gactgcaaag acatcttcaa agtccacctg
     8221 cactccgtcc aatacacctg catcctcagc tacaacgaac acgccctaaa agtatgggaa
     8281 ggcttgttaa acacagaaag ctacctgccg accatttcag atcccgacaa agcactttgg
     8341 gttgccgaat acaaaggcaa catacaggga tttttccaaa tcgactgcca agaagcacaa
     8401 ctcgatgcgc tttacgtcca ccccctgttc cacaacctcg gactgggcac tgccctactt
     8461 catcaggcgg aaaccatcgc ccacaaatcc ggtttgagct ttctgaaact ctacgcatcg
     8521 ctcaactccg tcccgtttta ccttctcaac cgctacgaat cgctcggttc ggcagtattg
     8581 cagcttgacc ccagcatcaa aatcaaatgc gaactgatgc gcaaacacct ctaatcccac
     8641 cttataacca aaactgataa tcttcaaatc aaacattatt tccacaagcg cagacagccg
     8701 tataaagttg ccccatctac caaccgacag gacaagcata tgaaaaccta cgcaccggca
     8761 ctctataccg cagccctgct caccgcctgc agccccacag ccgattcaaa ccatccgtcc
     8821 ggacaaaatg ccccggccaa taccgaatcc gacggaaaaa acattaccct gctcaacgcc
     8881 tcatacgatt tggcacggga tttttacaaa gaatacaacc ccttatttat caaaacatac
     8941 caatccgaac accccggcac atccgtcagc atccaacagt cccacggcgg ctccagcaaa
     9001 caggcattat ccgtagccaa cggccttcaa gccgatgtcg taaccatgaa ccaatcctcc
     9061 gacatcgacc tgctcgaaaa aaaaggactg gtagaaaaag gctggcaaca agccctcccc
     9121 gaccacgccg cgccctacac cagcactatg gttttccttg tccgaaaaaa caaccccaaa
     9181 cagatccgcg attggaacga ccttgccaaa gacggcgtta acatcgtcat cgccaatccc
     9241 aaaacctcgg gcaacggacg ctacgccttc ctcggcgcat acggttacgg tctgaaaacc
     9301 accaacggca acgaacagga agcccaaaaa ctcgtcgcat ccatcctcaa aaacaccccc
     9361 gtttttgaaa acggcggacg cgccgccacc accaccttca cacaacgcaa catcggcgac
     9421 gtactcatca cttttgaaaa cgaagccaac tacgtcagca aaaaactgac ccaaggacag
     9481 tttgaaatcg tctatccgag ctacaccatt tccgccgaaa gccccgtcgc cgtcgtcaac
     9541 agcatcgtcg cgaaaaaagg cacgcaaaaa accgcccgcg cctatctcga atacctttgg
     9601 agcgaaccgg cgcaagaact cgccgcctca ctctacctgc gcccccgcaa ccccgaagta
     9661 ttggcaagac acaaagccga cttcccagac ttagacacat tctctcccga agaaaaattc
     9721 ggcggatggg acaacatcat gaaaacctac ttcgccgacg gcggcatatt cgaccgcctg
     9781 accgcacaaa aatgaccgct atgccgtctg aaccgccttt cagacggcat ttcacacatc
     9841 aaaacagaaa aaccgcgccc aaaatcatca tcacaagaca caacggcaaa ccggatacaa
     9901 aacccaacac cgccgccaaa atgccgtctg aagccgtcgg cgcaaccatc cttatctggg
     9961 gcataacttt ccttaaccat cccttttgac agccaaataa acagtgcttt tgcctgtcag
    10021 aaacaaaacc aatattttgc agttcgtatc ggaacattta tagtggatta acaaaaacca
    10081 gtacagcg
//
LOCUS       DAWFHY010000073         9705 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.073, whole genome shotgun sequence.
ACCESSION   DAWFHY010000073 DAWFHY010000000
VERSION     DAWFHY010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.073"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..667
                     /locus_tag="WGQ68_001607"
     CDS             <1..667
                     /locus_tag="WGQ68_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0715379.1"
                     /translation="PDYSTLCRYRNRLAQDNTLSELLELINRQLTEKGLKVEKASAAV
                     VDATIIQTAGSKQRQAIEVDNKGQVSGQTTPSKDKDARWTKKNSLYKLGYKQHTRTDA
                     EGYIEKLYITPTNAHECKHLSPLLEGIAEGTTVYADKGYDSAENRQHLEEHQLQDGIM
                     RKACRNRPLTETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLICARCRLKGS
                     PDA"
     gene            complement(923..1135)
                     /locus_tag="WGQ68_001608"
     CDS             complement(923..1135)
                     /locus_tag="WGQ68_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0715380.1"
                     /translation="MELGYTPYNLRTLRNRCKLTQAELAQIVGVKHYIQVGRWEAEPD
                     TETRRADMPLEKWRQFLDWTEKTNAV"
     gene            complement(1147..1428)
                     /locus_tag="WGQ68_001609"
     CDS             complement(1147..1428)
                     /locus_tag="WGQ68_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715381.1"
                     /translation="MNRAALFNRYPEWIIGQDGASRFITHCRYPRLIAKIHRQTDSEC
                     PGGHYRHSENGITLYDFIFFDGKPADEARFAAVLTETCRRAVKKICETK"
     gene            complement(1475..1738)
                     /locus_tag="WGQ68_001610"
     CDS             complement(1475..1738)
                     /locus_tag="WGQ68_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715382.1"
                     /translation="MADILPSDKRRQPPKTKGSTMNIIEQEMKHSSLRKVDAEIAKII
                     ADAHKINAESVKIAQESRWYPMMAATGLVTAIAAVLALIFKFA"
     gene            complement(1758..2332)
                     /locus_tag="WGQ68_001611"
                     /pseudo
     CDS             complement(1758..2332)
                     /locus_tag="WGQ68_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(2307..2651)
                     /locus_tag="WGQ68_001612"
                     /pseudo
     CDS             complement(2307..2651)
                     /locus_tag="WGQ68_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701232.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2644..3519)
                     /locus_tag="WGQ68_001613"
     CDS             complement(2644..3519)
                     /locus_tag="WGQ68_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3102 domain-containing protein"
                     /protein_id="HEZ0715383.1"
                     /translation="MNTEIEIIATDKVSNQAAMRSVLVMEQWGNGETYSEERWVERGR
                     QAVRQTMEGMFELGRALIILKEHTEHGRFMEIVKSQFGLGIAETSRLMSATRRFATPQ
                     MQKAAPKLMDLGKSKLLELLVEEDVTLVGLAEGEEVNGMTFDDVDRMTVRELRVALRE
                     SRENLAAKDEVMKTKTAKIDELTEKLAKKQTVVREPKAEDVGSELAMQLTSLEVGIRS
                     QVSRLKDLFDQLNAHSEAHEISHQAKMVGTLNQIILDCEQLRESYALPTEAPTDNVPE
                     WLGGETGEGDESGND"
     gene            complement(3564..3854)
                     /locus_tag="WGQ68_001614"
     CDS             complement(3564..3854)
                     /locus_tag="WGQ68_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0715384.1"
                     /translation="MAESKRVQRLLRVFIALDEHPIIGLSNKDLSVGLGLTPSQVSRD
                     IDDLVASGLVIKLENGNYAYGIKTLQIAERFRQQHERLQSKIAEIGKRVDVD"
     gene            complement(3866..4042)
                     /locus_tag="WGQ68_001615"
     CDS             complement(3866..4042)
                     /locus_tag="WGQ68_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0715385.1"
                     /translation="MAITVEKLKENFAKNGQTLAQWARENGFKPRDVYLVVGGQRKGN
                     YGKGHEIAKKLGLK"
     gene            4160..4531
                     /locus_tag="WGQ68_001616"
     CDS             4160..4531
                     /locus_tag="WGQ68_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ0715386.1"
                     /translation="MLFVGFLERIKQELDLKKDKDVAEFLGLEPKAFSIRKKRDSIPV
                     DAVRAAAFEHPELNADWGYILTGRREKSVKMPSEKLNKEEQELLALFREAAAADREMI
                     LMVARRAEKKAQAALGKVSNG"
     gene            4536..4838
                     /locus_tag="WGQ68_001617"
     CDS             4536..4838
                     /locus_tag="WGQ68_001617"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715387.1"
                     /translation="MNTVLTPKLIEKLERLRSLEIEAQELRAELQPWLDNACKQYFVA
                     WLENQLEKGRDISVLELKSNLSDLAYHAVMNDNIALWHQVSEVKKLLAIYEAKRKT"
     gene            5058..5264
                     /locus_tag="WGQ68_001618"
     CDS             5058..5264
                     /locus_tag="WGQ68_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019389005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
                     /protein_id="HEZ0715388.1"
                     /translation="MMFASPTPALAKSCKDFPTQQAAQKFYEARKKAGLSGWKSLDRD
                     GDGWACDCNPGGSGKNCPKKKDNF"
     gene            5275..5862
                     /locus_tag="WGQ68_001619"
     CDS             5275..5862
                     /locus_tag="WGQ68_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715389.1"
                     /translation="MKTKIYLGIFLLAAAHLVNAGGVMKTGGMIGPHPETFSEQFNEL
                     NNGMMDLFPRISIVAGKTRQTKDLGNGITLSAKLVKEGDIFENVRINCNTLAKTQRIN
                     NCLVSMYYTAIALDYEIDRLAFMNAIQTAIKRMHATYNQNGVDYFITVNRKQKSVSMF
                     AKSDMPMQIEAMDDSNEAYMKSDKCNPEIELCIKP"
     gene            5957..6460
                     /locus_tag="WGQ68_001620"
     CDS             5957..6460
                     /locus_tag="WGQ68_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 108 protein"
                     /protein_id="HEZ0715390.1"
                     /translation="MSDEFNRFIERVLSHEGGYANHPKDPGGETNWGITKRTAQANGY
                     NGSMRAMTREQAIGIYRKAFWERYRADQMPEAVAFQFFDACVNHGYGNAVRMLQRAAG
                     VADDGIIGEISLKAINSLPENDLLLRFNAERLVFYTKLSTFTSFGKGWVRRVAQNLIH
                     ASADNTD"
     gene            6572..6910
                     /locus_tag="WGQ68_001621"
     CDS             6572..6910
                     /locus_tag="WGQ68_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715391.1"
                     /translation="MLDASPPQERGSRRETRSQKKASQITAFFRWLGGLVSNPATGKI
                     SHTKLWANVAAAAMTWKFVQAADAPEWLWWAYGALVGGYALIKRGIAAVPQLAEIKKS
                     ANAEEWSGND"
     gene            6903..7313
                     /locus_tag="WGQ68_001622"
     CDS             6903..7313
                     /locus_tag="WGQ68_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HEZ0715392.1"
                     /translation="MIEFVRAKKRLLWAFVLLLVWTCGYRYAADKAEAEQTALIATYR
                     HSSMVAAEQYALQLKKAQDERQRWYDFSQKQGRKPVKKQYPPQTKKAGYLKTKEELLA
                     ELACLTAEMAALKKPDALIHGKEVRQKERNSSQG"
     gene            7318..8067
                     /locus_tag="WGQ68_001623"
     CDS             7318..8067
                     /locus_tag="WGQ68_001623"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEZ0715393.1"
                     /translation="MPSVELLLEIVGLPRGTFYYQLAVQSAEDKYADLKQHIHDIYRR
                     HKGRYGYRRIAAAILHAGTPVNHKKVSRLMAKTGLKAVIRRRKYRSFKGEVGKIAPNI
                     LRRCFHAEKPNEKWVTDVTEFNVGGEKIYLSPIMDLFNGEIVSYRIQIRPTFDLAGEI
                     LKGAPEKPGSSEKPILHSDQGWQYQMFFYSGLNLNQDKAMKPQTVQIVRQGEATPYWF
                     KFNPLYNNDRIKLKLKGLSLVQYRIQSLKAA"
     gene            complement(8386..8748)
                     /gene="ssrA"
                     /locus_tag="WGQ68_001624"
     tmRNA           complement(8386..8748)
                     /gene="ssrA"
                     /locus_tag="WGQ68_001624"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(8811..9416)
                     /locus_tag="WGQ68_001625"
     CDS             complement(8811..9416)
                     /locus_tag="WGQ68_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEZ0715394.1"
                     /translation="MNTDFQNILKQLGKQAQKEAAEKQAAEKNKQKQEQDFDFSQAVG
                     QVSPLKNRQQYYAPPDKTPIKVRPKDNRADEENYFYIGSTYNDPPASFSKNGQGKNDI
                     QRLKNGYYPVVTDVDLHGYTQEEAQKVLNEFIAFTQKRGVCGEIIHGSGLGSKGYKPV
                     LKNMTRNWLMQHPDVLAYAEPREGNDGCVRILLKRKLRQQD"
BASE COUNT         2335 a         2286 c         2568 g         2516 t
ORIGIN      
        1 ccccgattac agcaccttat gccgctaccg caaccggctg gcgcaagaca ataccctgtc
       61 tgaactgttg gaactgatta accgccaact gaccgaaaaa ggcttaaaag tagagaaagc
      121 atccgccgcc gtcgttgacg ccaccattat tcagaccgcc ggcagcaaac agcgtcaggc
      181 tatagaagtc gataacaaag gacaagtcag cggccaaacc acaccgagta aggacaaaga
      241 tgcccgttgg acaaagaaaa acagcctcta caaactcggt tacaaacaac atacccgtac
      301 cgatgcggaa ggctatatcg agaaactgta catcactccc accaatgccc atgagtgcaa
      361 acacctgtcg cctttgctgg aagggatagc cgaaggtacg accgtctatg ccgacaaagg
      421 ctacgacagt gcggaaaacc ggcaacatct ggaagaacat cagttgcagg acggcattat
      481 gcgcaaagcc tgccgcaacc gtccgctgac ggaaacgcaa accaaacgca accggtattt
      541 gtcgaagacc cgttatgtgg ttgaacaaag cttcggtacg ctgcaccgta aattccgcta
      601 cgctcgggca gcctatttcg gactgatttg cgcccgctgc cgcctaaaag gcagcccgga
      661 tgcctgatta tcgggtatcc ggggaggatt aagggggtat ttgggtaaaa ttaggaggta
      721 tttggggcga aaacagctga aaacctgtgt ttgggtttcg gctgtcggga gggaaaggaa
      781 ttttgcaaag gtctcagagt gagtttattt tggggcggcg gcaggtcggg ggcaagcggc
      841 gtggggcttg gttgtggttt ttaggtttgg gggtgggtaa aaaatgccgt acccggcggg
      901 caggcttcgc actctgaagg tttcagacgg cgtttgtttt ttctgtccaa tcgaggaact
      961 gccgccattt ttccagcggc atatcggccc gccgcgtttc ggtatcgggt tcggcttccc
     1021 agcggcctac ctgtatgtag tgcttcaccc cgacgatttg cgccaactcg gcctgtgtca
     1081 gtttgcagcg gttgcgcagg gtgcgaaggt tgtaaggcgt gtagccgagt tccatgtcgt
     1141 tgcggtttat tttgtttcgc atattttttt gactgcccgg cggcaggttt cggtaaggac
     1201 ggcggcaaat cgggcttcgt ctgccggttt tccgtcgaaa aaaataaaat cgtaaagggt
     1261 gatgccgttt tcggaatggc ggtaatgccc gcccgggcat tcgctgtctg tctgcctgtg
     1321 gattttggca atcaggcggg gatagcggca atgggtaatg aaacggcttg cgccgtcttg
     1381 cccgataatc cattcggggt agcggttgaa tagtgcggct ctgttcattt tgttcgggtt
     1441 gttcggggga taaagcccct cgcggggctt gtggtcaggc aaatttgaat atcagtgcca
     1501 acacggcggc gatggcggta accagcccgg tggccgccat catgggatac catcgggatt
     1561 cttgggctat ttttacggat tcggcgttga ttttgtgcgc gtctgcaatg attttggcga
     1621 tttcggcatc tacttttctc agactggagt gtttcatttc ttgttcgatt atgttcatcg
     1681 tacttccttt cgtttttggc ggttgccgcc gcttgtcgga tggtaggatg tctgccatgt
     1741 gtatatattg atacctttta ggttttattg caagtgtttt gggcggcggc ttcgtatgct
     1801 tggcggtggc ggcggctgta ccattcggcg agttcgcgcc gctcttggag gcggtagctg
     1861 tcggcggtca ggtagtggtg caggcttgag aagccggcgc gttggagggc ggcgcggctg
     1921 atgtggccga gggcgaggtc tgttgtgagg gtgtcttttc cggcggtcag gctgatgttg
     1981 gtgaagaggt ggcggaatcc gtgcattgtg tgtttggatt tgccgggggt gctgccgtca
     2041 tagcccagtc gtcggatggc gttgtgggcg aatttgatgc tgatgtggtc gggatggggg
     2101 gggcgggttt gcgccgtggg cggatgccgg ggaaaaggtg gatgttgtcg ccggtctgcg
     2161 tgtgcagctc tcggagtatt tctaccgccc agtccgacag tgggacggta aaggggtgtt
     2221 tggtcttcat gtcggcggcg gggatgtgcc ataaccgggc ggtgaggtcg atgtcttgcc
     2281 agcgggcgga aagcagtgcg gacggacggg atgacttccc cggtttcccc atcaatccgc
     2341 gccgcctcga tttttccgtt ggtgcgcagc atttccaccg cctgccgcac cgacatcaac
     2401 cgcttgccgt tgcgcctcat cgcctccacc aaaaccgccg aaatcaattt ggcttcttcc
     2461 gttttaagct ccgtcttgcc cgcatcgctg cgccgtttgc gcgtcggctt gatgctgacc
     2521 gcctccagct tgcggtatag cgtggcaagg ctgatgccca attcctgcgc ctgctgctta
     2581 agatatgcag agcgtgcgcc gcgtcccatt gcttccgcct gattctcgac tgccttaaga
     2641 cgctcaatca ttgccggatt catcgccttc tcccgtttca ccgcccaacc attccggcac
     2701 attgtctgtc ggtgcttcag tcggcagggc atagctttcg cgcagttgct cgcagtccaa
     2761 aataatttga ttgagcgtgc cgaccatctt tgcctgatgg ctgatctcgt gtgcctcact
     2821 gtgcgcatta agttggtcga acaaatcttt caaacggctc acttgactgc ggataccgac
     2881 ctcaaggctt gttaactgca tcgccaactc actgcccacg tcttccgcct tcggctctct
     2941 gacaacggtt tgcttcttag ccagcttctc ggtcagctca tcaattttgg ctgttttggt
     3001 tttcatcact tcgtctttgg cggcgaggtt ttcacggctt tcgcgcaggg cgacgcgcag
     3061 ctcgcgcacc gtcattcggt ccacatcgtc aaaggtcatg ccgttgactt cttctccttc
     3121 ggcaagtccc accaacgtaa cgtcttcttc gaccaagagt tccagcagct ttgacttgcc
     3181 caaatccatc agcttcggcg cggctttctg catttgcggg gtcgcaaagc ggcgagtggc
     3241 tgacatcaga cgtgatgttt ctgcgatacc cagcccaaat tggcttttca caatttccat
     3301 aaaccgtccg tgttccgtat gctcttttaa aataatcagc gcacgtccca gttcgaacat
     3361 gccttccatc gtctgccgta ctgcttgacg accgcgttca acccaacgct cttcgctata
     3421 agtctcgccg ttaccccact gctccatcac cagcacactg cgcatcgctg cctgattgct
     3481 tactttgtcg gttgcgataa tttcaatttc tgtattcatt ttttatctcc aaagtttccg
     3541 acgtcggaaa ctttcaaaat ccgttaatcc acatcgaccc gcttgccgat ttcggcaatc
     3601 ttgctttgca gccgttcatg ctgctgcctg aaccgctctg cgatttgcag ggttttgatg
     3661 ccgtaggcgt agttgccgtt ttcaagtttg atgaccaatc ccgaggcaac caaatcatca
     3721 atatccctgc tgacttgcga tggcgtcagc cccagtccga ccgataaatc cttattgctc
     3781 agaccgataa tcggatgctc gtcaagcgcg ataaagaccc tcaatagccg ttgtaccctt
     3841 ttactttctg ccatccgcat cctccttatt tcagtcccag cttcttggca atttcatgcc
     3901 ccttgccgta attgcctttg cgctgtccgc cgaccaccag atacacatcg cgcggcttaa
     3961 agccgttctc tcttgcccac tgcgccagcg tctggccgtt tttggcaaaa ttttctttta
     4021 gtttttcaac agttatagcc atagatagcc ctttcccatt gtgataaaat agtgaactat
     4081 ttatgcacca gatatttact agtgcgctcc ataaacagaa tattaatcta cttttgtaga
     4141 ttatttcaag agattttgta tgctttttgt gggtttttta gaaagaatta agcaagagct
     4201 tgatttaaaa aaggataagg atgtcgctga atttttagga ctagagccga aagcgttctc
     4261 aattcgaaaa aaacgcgact cgataccagt agatgcagta cgcgcagcag catttgaaca
     4321 tccggaatta aatgctgatt ggggatatat cttgactgga cggcgagaaa aatcagtaaa
     4381 aatgccatct gaaaaattaa acaaagaaga gcaggagctg ctggccttgt tccgcgaagc
     4441 ggcagctgcc gaccgtgaaa tgattctgat ggttgcgcgc agggcagaga aaaaagccca
     4501 agctgcgctt ggtaaagtga gcaatggata atgatatgaa tacggtgttg acgcccaaat
     4561 taatagaaaa attagaacga ttaagaagcc ttgaaattga ggcacaagag ttgagagcgg
     4621 agctgcaacc ttggttagat aatgcttgca aacaatattt tgttgcatgg cttgagaatc
     4681 aactggaaaa aggtcgggat atttctgttt tggagctaaa aagcaatttg tcagatttag
     4741 cttaccatgc ggtgatgaat gacaatatcg cattgtggca tcaggtaagt gaagtgaaaa
     4801 aattgcttgc tatttatgaa gctaagcgga aaacataaaa aatgccgtct gaaattttca
     4861 gacggcattt aggacactcc gacagttggg ctcggtgttc tgaaacggat attaagcaat
     4921 tgaagcggcc gtgcatttta aggcgcatta aaaaccaccc cgatattcag gtcatcgggc
     4981 aggtggttca ggtctcgaaa aacctgtatt aacagagaca aaatgaaaag ggttatttga
     5041 cattgcttgt gtctgttatg atgtttgctt cacccacgcc cgccttggcg aaaagctgca
     5101 aagactttcc gacgcagcag gcggcgcaga aattttatga agcccgcaaa aaagcaggat
     5161 taagcggctg gaagagtttg gacagggacg gcgacggatg ggcttgtgac tgcaacccgg
     5221 gcggaagcgg caagaattgt ccgaaaaaaa aagataattt ctgaaagaaa tagaatgaaa
     5281 acaaaaatat atcttggaat tttcttattg gctgctgcac atctggtcaa tgcgggagga
     5341 gtgatgaaaa caggagggat gattggtcct catcctgaaa cattttcgga acagttcaat
     5401 gaattgaaca atggaatgat ggatttgttc ccaagaatat caattgttgc gggcaagaca
     5461 cgtcaaacaa aagaccttgg taatggcatt acgttgtctg caaaactggt caaggaagga
     5521 gatatttttg agaacgtccg gataaattgt aatacattgg cgaaaactca aagaatcaac
     5581 aattgcttag tgagtatgta ttacacggct attgcgttgg attatgagat agaccgtttg
     5641 gcatttatga atgctattca gactgctatc aaaaggatgc atgctactta taatcaaaat
     5701 ggggtagatt atttcattac ggtcaatcgt aagcagaaaa gtgtttctat gttcgcaaaa
     5761 tcggatatgc ctatgcagat tgaggcaatg gacgattcaa atgaagcata tatgaaaagc
     5821 gataaatgca atcccgaaat cgagctgtgt attaaacctt agcctcagga aggttttaac
     5881 ctccattaaa agcccgttca gacggccttt cctacaatcc ccgtattgat ttcaacctca
     5941 atacggggat ttttccatgt cagacgaatt caaccgattt atcgaacgcg tcctctccca
     6001 cgagggcggt tacgccaacc atcccaaaga ccctggcggc gaaaccaatt ggggcatcac
     6061 taagcgcacc gcacaggcaa acggctacaa cggctccatg cgtgccatga cgcgtgaaca
     6121 ggctatcggc atttaccgaa aggcgttttg ggagcgttac cgcgccgacc aaatgccgga
     6181 agcggtcgcg ttccaatttt ttgatgcctg cgtcaaccac ggttacggca atgccgtccg
     6241 tatgctgcaa cgcgccgcag gcgtggcgga cgatggaatc atcggcgaaa tcagcctcaa
     6301 agccatcaat tccctccccg aaaacgacct tttattgcgg ttcaacgccg agcgtctggt
     6361 cttttatacc aagctcagta cgttcacctc tttcggcaag ggctgggtac gccgtgtggc
     6421 gcaaaacctg attcacgcgt ctgcagataa cactgattaa agggagataa accatgtcaa
     6481 aaaagtcact catcgcccta atgaccgcag ccatgcagcc cgatttcagc cacagcgacc
     6541 taggcattcg ctacgccatg ccgactcagg gatgttggac gcaagcccac cgcaagagcg
     6601 gggtagccgc cgcgaaacgc gcagccaaaa aaaagcgtca caaataaccg cctttttccg
     6661 atggctgggc ggcttggtct ctaatccggc cacaggaaaa atcagccata ccaaactatg
     6721 ggcaaacgtc gccgcagccg ccatgacttg gaagttcgtg caggcggcgg acgcgcccga
     6781 atggctctgg tgggcttatg gcgcattggt cggcgggtat gcattaatca aacgcggcat
     6841 cgccgccgta ccgcagttgg cagaaatcaa aaagtccgcg aatgcggaag aatggagcgg
     6901 caatgattga atttgtccga gccaaaaaac ggctgctttg ggcatttgtg cttttgcttg
     6961 tgtggacgtg cggttaccga tacgccgccg acaaggccga agcggaacaa accgccctga
     7021 ttgccaccta tcggcattct tctatggttg cggcggaaca atatgccttg cagcttaaaa
     7081 aagcgcagga cgaaaggcag cggtggtacg acttttccca aaaacaagga agaaagcccg
     7141 tgaaaaaaca gtatccgccg caaacgaaaa aagccggcta tctgaaaacc aaggaagaac
     7201 tgcttgcgga attggcttgc cttacagcgg aaatggctgc cctaaaaaag cccgatgcct
     7261 taatccatgg gaaagaagtg cggcagaaag aacgcaactc gtcgcagggt taaggcaatg
     7321 ccatccgttg aactgctgtt ggagattgtc ggactgccac gcggcacctt ctattaccaa
     7381 ttggccgtcc aatcggcaga agacaaatat gccgatttga aacagcatat ccatgatatt
     7441 tatcgacgac ataagggaag atacggctac cggaggattg cagcagccat ccttcatgca
     7501 ggaacaccgg tcaatcacaa gaaagtcagc cgtctgatgg cgaagacggg gctgaaggca
     7561 gtgatacggc ggcgcaaata ccgctcgttc aaaggagaag tcggcaaaat tgcgccgaat
     7621 atcctgcgac gctgtttcca tgcagaaaag ccgaatgaga aatgggtaac ggacgttacc
     7681 gagttcaatg taggcggaga aaagatatac ctttctccga ttatggattt gtttaacggg
     7741 gaaatcgtca gttaccgtat tcaaatccgc ccgactttcg atttggccgg cgagatactg
     7801 aaaggtgcgc cggagaaacc gggatcgtct gaaaagccga tactgcattc ggatcaaggt
     7861 tggcaatatc agatgttttt ttatagtgga ttaaatttaa atcaggacaa ggcgatgaag
     7921 ccgcagacag tacaaatagt acggcaaggc gaggcaacgc cgtactggtt taaatttaat
     7981 ccactataca acaacgatag aatcaagttg aaattaaaag ggctgagcct tgttcagtac
     8041 agaattcagt ccctgaaagc cgcttgatta aactgtccga ctttttgggg tcagttcggc
     8101 ttcggcattt ttttatccgt ttgggggtaa cttgtttgga aagctgcaag cttataaata
     8161 aaggattaca tttaagtttt gggtaacctt tttaaaaaaa tgcgtgatga cttttgcatt
     8221 tttaaggcgt tttttggggt aattcgtgaa aagttacccc aaaagttacc ccataaatgg
     8281 cgaaaactca agcatacgcc agcatattca aggcaacaaa aaagccttga aactgttgaa
     8341 gttcaaggct tttttgtgtt gcaggatgct gctgaaaata gggtatggtg gaggcggggg
     8401 gaatcgaacc cccgtccgaa agtcctctac aaagcgttct acatacttag ttgtgtctat
     8461 ttgaaaatct tatttccatc atgccgacca acaggccttt tggaaaccag ttaccttaaa
     8521 tctcatttcc tgccaagtaa cccggtagga aaccagtcaa tgtaagatga cgttgcggtg
     8581 gctttcgcca cacagcccat tgaccgactg ctgcaacggc tagccttaag cggctaaagc
     8641 gtaagtttcg tcgtttgcga ctatttgaat tcagtgtttt acgggaatct gagaccccgg
     8701 tatgcccgca tctgcttcgc aacccccgtc gaaaccaagg tcgcccccag aaatggtttg
     8761 caaattatac ggatattgtg cggtgctgcc aagtctgtcg gagaaatttg tcagtcttgc
     8821 tgccttaatt tgcgtttgag caggatgcgg acgcagccgt cgttgccttc ccggggttcg
     8881 gcgtaggcga gtacgtcggg gtgttgcatc agccagtttc gggtcatatt tttcagaacg
     8941 ggtttgtagc ctttggaacc taatccgctg ccgtggatga tttcgccgca tacgccgcgt
     9001 ttttgggtga atgcgatgaa ttcgttgagg actttttggg cttcttcctg tgtgtagccg
     9061 tgcaggtcga catcggtaac gacgggatag tatccgtttt tcaggcgttg gatgtcgttt
     9121 tttccctgtc cgtttttgct gaagctggcg ggcgggtcgt tgtatgtgct gccgatgtag
     9181 aagtagtttt cttcgtcggc gcggttgtct ttgggacgga ctttgatggg ggttttgtcg
     9241 ggcggcgcat aatattgctg ccggtttttt aatggggaga cttgtccgac tgcttgtgaa
     9301 aaatcgaaat cctgttcttg tttttgcttg tttttttcgg ctgcctgttt ttctgccgct
     9361 tctttttggg cttgtttgcc cagttgtttg aggatgtttt ggaagtcggt attcatattt
     9421 tttcctgtta tttgtccgat ggctgtttcg ggcggggttt taatttgccg gaatgtttgc
     9481 caatcggggg aggatgattt tgttgcctgc gtatgttttt tgaaagtgtg attgtatatc
     9541 aaaaagaaat gcggcaaccg tcggcagtgt tgattgccgg aaatgcggac cggtcgaacc
     9601 gatatgcccg aacgcctgat aaagttttaa aaacctgcct tgcgaagcag gctgacgtgt
     9661 tttgccaatc ttgaattgcc ggaaacgcga aacacggaaa tctga
//
LOCUS       DAWFHY010000074         9687 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.074, whole genome shotgun sequence.
ACCESSION   DAWFHY010000074 DAWFHY010000000
VERSION     DAWFHY010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9687
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.074"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(45..3089)
                     /locus_tag="WGQ68_001626"
     CDS             complement(45..3089)
                     /locus_tag="WGQ68_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HEZ0715395.1"
                     /translation="MFWIVMIVILLLALAGLFFVRAQSEREWMREVSAWQEKKGEKQA
                     ELPEIKDGMPDFPELALMLFHAVKTAVYWLFVGVVRFCRNYLAHESEPDRPVPPASAN
                     RADVPTASDGYSDSGNGTEEAETEEAEAAEEEAADTEDIATAVIDNRRIPFDRSIAEG
                     LMPSESEISPVRPVFKEITLEEATRALNSAALRETKKRYIDAFEKNETAVPKVRVSDT
                     PMEGLQIIGLDDPVLQRTYSHMFDADKEAFSESADYGFEPYFEKQHPSAFSAVKAENA
                     RNAPFHRHAGQGKGQAEAKSPDVSQGQSVSDGTAVRDACRRVSVNLKEPNKATVSAEA
                     RISRLIPESRTVVGKRDVEMPSETENVFTETVSSVGYGGPVYDEAADIHIEEPAAPDA
                     WVVEPPEVPKVPMPAIDIPPPPPVSEIYNRTYEPPSGFEQAQRSRIAETDHLADDVLN
                     GGWQEETAAIADDGSEGAAERSSGQYLSETEAFGHDSQAVCPFENVPSERPSRRASDT
                     EADEGAFQSEETGAVSEHLPTTDLLLPPLFNPEATQTEEELLENSITIEEKLAEFKVK
                     VKVVDSYSGPVITRYEIEPDVGVRGNSVLNLEKDLARSLGVASIRVVETIPGKTCMGL
                     ELPNPKRQMIRLSEIFNSPEFAESKSKLTLALGQDITGQPVVTDLGKAPHLLVAGTTG
                     SGKSVGVNAMILSMLFKAAPEDVRMIMIDPKMLELSIYEGIPHLLAPVVTDMKLAANA
                     LNWCVNEMEKRYRLMSFMGVRNLAGFNQKIAEAAARGEKIGNPFSLTPDDPEPLEKLP
                     FIVVVVDEFADLMMTAGKKIEELIARLAQKARAAGIHLILATQRPSVDVITGLIKANI
                     PTRIAFQVSSKIDSRTILDQMGAENLLGQGDMLFLPPGTAYPQRVHGAFASDEEVHRV
                     VEYLKQFGEPDYVDDILSGGGSEELPGIGRSGDGETDPMYDEAVSVVLKTRKASISGV
                     QRALRIGYNRAARLIDQMEAEGIVSAPEHNGNRTILVPLDNA"
     gene            3254..3934
                     /locus_tag="WGQ68_001627"
     CDS             3254..3934
                     /locus_tag="WGQ68_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HEZ0715396.1"
                     /translation="MIFQTVWFSDMVLSVSWIVLILILAASAPSAFRSLDRYRSALPL
                     CTVIFSAAWCLNASAGGGQLAQMNYHLLAVNLVALMVGTSAALWLAALLMLPYCLLFA
                     GSVGAYPPNALVLILPALVVNRLSRMLVNRLPPNIFIFIFVNGFLASAAGILLTGLVL
                     TGILDAANAFPSEILWTTALPVFILLAWAEAFLSGISTAIFVALKPHWINTFDDNRYL
                     KSDRSIWR"
     gene            3963..4322
                     /locus_tag="WGQ68_001628"
     CDS             3963..4322
                     /locus_tag="WGQ68_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEZ0715397.1"
                     /translation="MLSNIIPLSIGAALGATARWLLNLAVPAAMSPATGNLFANWTGA
                     LLIGIFAETVNHPQWKLLLITGFLGSLTTLSGFSLETVTLLQSNRPASALSNIFLHTA
                     GSLLLTWLGLKIGTAVK"
     gene            complement(4454..4882)
                     /locus_tag="WGQ68_001629"
     CDS             complement(4454..4882)
                     /locus_tag="WGQ68_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715398.1"
                     /translation="MISIFKAYYDVYEFNGELLKRCIFLDEENEENNEIIKDRIYEYL
                     IYVLTGDLYLQKDIDENLEFIHQAENNPNEVYSGGGQGFCWDISAEKVVFYNNEFDEE
                     DGWPDLSCSLHTFKTTLIAWNAFLQLPKSIHSVVETVIEE"
     gene            complement(5401..5937)
                     /locus_tag="WGQ68_001630"
     CDS             complement(5401..5937)
                     /locus_tag="WGQ68_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEZ0715399.1"
                     /translation="MDLREVKLGGETIYEGGFVSISRDKVRLPNGNEGQRIVIRHPGA
                     ACVLAVTDEGKVVLVRQWRYAANQATLELPAGKLDVASEDMAACALRELAEETPYTAD
                     SVRLLYSFYTAVGFCNEKMYLFEAEGVRLGSTLANDEDEITETVLMSKEEVRQALAND
                     EIKDGKTLIGLQYWLMKD"
     gene            complement(5971..6327)
                     /gene="folB"
                     /locus_tag="WGQ68_001631"
     CDS             complement(5971..6327)
                     /gene="folB"
                     /locus_tag="WGQ68_001631"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEZ0715400.1"
                     /translation="MDKIFLHGMKADTLIGVYGWERERLQTLIVDLDIGVPEKAGSDD
                     DIANTVHYAEVCETLRRHLKEQDFLLLEALAEYIADLVLGYFGAVWVRVKIVKPGILE
                     GVREVGVEIERGKRED"
     gene            6383..6985
                     /gene="plsY"
                     /locus_tag="WGQ68_001632"
     CDS             6383..6985
                     /gene="plsY"
                     /locus_tag="WGQ68_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEZ0715401.1"
                     /translation="MFNISAVAVSYLIGSLSFAVIVSKYYGMDDPRTYGSGNPGATNV
                     LRSGKKKAAALTLLGDAAKGLVAVLLARVLQEPLGLSDSAIAAVALAALVGHMWPVFF
                     GFKGGKGVATALGVLLALSPATALVCALIWLVMAFGFKVSSLAALTATIAAPLAALFF
                     MPHTSWIWATLLIALLVLFRHKSNIVKLLEGRESKIGGSR"
     gene            7062..7907
                     /locus_tag="WGQ68_001633"
     CDS             7062..7907
                     /locus_tag="WGQ68_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HEZ0715402.1"
                     /translation="MLSYRHAFHAGNHADMLKHFTLFLVLQYFNRKDKPYWYIDTHGG
                     AGVYNLEGSKAQKVGEYRQGIALLRQAQNLPAELSDFAAHIQKILPSPKLYCGSPWLA
                     QSLTRVGDKLRLFELHPTDFVHLKNNMGEAGLGKRGQVLREDGYKGLISLLPPPPRRA
                     TVLIDPPYEEKQDYRRVTETLKAALKRFESGCYLIWYPCLSREESRKLPEELKKLLPD
                     NYLHAELHVHAPKADGFGMHGSGMFVINPPYLLAEQLAANLPALTRLLAQDEGARYLL
                     DSKIR"
     gene            8153..9127
                     /locus_tag="WGQ68_001634"
     CDS             8153..9127
                     /locus_tag="WGQ68_001634"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEZ0715403.1"
                     /translation="MDTLTRFLPEHLQQNQLPEALGGVLLSVVSACTEINAKVRLGAL
                     AGVLGMAGTGNIQGEDQKKLDVIANNIMIDTLKANPAVAGLASEEEDTFVSAGENGRY
                     LVLFDPLDGSSNIDVNISVGTIFSILEKPEGALATESFLQTGRQQLAAGYVLYGPQTQ
                     LVFTFGHGVYMFTLNAENEFVLTKEKPKVPESTKEFAINMSNRRHWLPPVQQYIDELL
                     AGETGTRGKNYNMRWVASMVAEIHRILMRGGVFMYPQDKRDPAKPGKLRLMYEANPMS
                     LILEQAGASASNAYQAMLDIQPENLHQRVAVFMGSSEEVDYLNRLHSK"
     gene            complement(9285..9503)
                     /locus_tag="WGQ68_001635"
     CDS             complement(9285..9503)
                     /locus_tag="WGQ68_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEZ0715404.1"
                     /translation="MFPEYRDLISKLKQENSRFARLFDEHNELDDKITGLVNNPVTSG
                     AETIDELKKAKLKLKDELYAILQKAAGK"
BASE COUNT         2296 a         2811 c         2185 g         2395 t
ORIGIN      
        1 gccgtctgaa acagtcttca gacggcattt ccatttgcaa aaaatcaagc attgtccaag
       61 gggacgagaa tcgtacggtt gccgttgtgt tccggtgcgg acacaatgcc ttccgcctcc
      121 atctggtcaa tcagacgcgc ggcgcggttg tagccgatgc gcaaggcgcg ctgtacgccc
      181 gaaatactgg ctttgcgcgt tttcaggaca acggatacgg cttcatcata catcggatcg
      241 gtttcgccgt cgccgctgcg cccgatgccg ggcagctctt cgctgccgcc gccgctcaaa
      301 atatcgtcaa catagtccgg ctcgccaaac tgtttcaaat attcgaccac gcggtgcacc
      361 tcttcatccg aggcaaacgc gccgtgaacg cgctgcggat aggcagtacc cggcggcagg
      421 aacagcatat cgccctgacc gagcaggttt tccgcgccca tttggtcgag aatcgtgcgg
      481 ctgtcgattt tgctggacac ttggaacgcg atacgcgtcg ggatgttcgc cttaatcaga
      541 cccgtgatga catcgacgct ggggcgttgt gtggcaagaa tcaaatggat gcctgccgcg
      601 cgggcttttt gggcgaggcg ggcaatcagt tcctcgattt tcttgcctgc cgtcatcatc
      661 aaatcggcaa actcatcgac cacgaccacg ataaacggca gtttttccaa aggttcggga
      721 tcgtcgggcg tgaggctgaa cggattgccg attttttctc cccttgctgc ggcttcggcg
      781 attttttgat tgaagcccgc aagattacgc acgcccataa agctcatcag gcggtagcgt
      841 ttttccattt cgttaacaca ccagttcagc gcgtttgccg ccagcttcat atcggtaacg
      901 acaggggcga gcaggtgcgg gatgccttcg taaatgctca attccagcat tttcggatcg
      961 atcataatca tacgcacgtc ttccggcgcg gctttgaaaa gcatagacag aatcatcgcg
     1021 ttgacaccca ccgatttgcc cgaaccggtc gtgccggcaa ccaataaatg cggtgctttg
     1081 cccaagtcgg ttacaacggg ctgtccggtg atgtcctgac cgagcgcgag cgtcagcttg
     1141 gatttggatt cggcaaactc gggcgaattg aagatttcgc tcaggcgtat catttgacgt
     1201 ttcgggttcg gaagttccaa acccatacag gttttgccgg ggatggtttc gacaacgcgg
     1261 atggaagcca cgccgagcga acgcgccaag tctttttcca gatttagaac ggaattgccg
     1321 cgcacgccga catcgggttc gatttcataa cgcgtaatca cggggccgga ataagaatcg
     1381 acaaccttga ccttgacttt gaactccgcc aatttttctt cgatggtgat gctgttttcc
     1441 aacagttctt cttcggtttg cgtcgcctcg ggattgaaca gcggcggcag aagcaggtcg
     1501 gttgtcggca ggtgttcgga taccgcaccg gtttcttccg attggaacgc cccttcatcc
     1561 gcttccgtat ccgatgcccg gcgggacggg cgttcagacg gcacattttc aaacggacaa
     1621 accgcctgac tgtcatgccc gaacgcttcg gtttccgaca gatattgccc gcttgaccgc
     1681 tctgccgcac cctcactgcc gtcatccgca atagcggcgg tttcctcctg ccaacctcca
     1741 ttcaaaacat catcggcaag atggtcggtt tcggcaatgc ggctgcgttg cgcctgctcg
     1801 aatcctgacg gcggttcata ggtacggttg tagatttccg atacgggagg tggcggcgga
     1861 atatcgattg cgggcatggg aactttcggc acttcgggtg gttcgaccac ccaagcatcg
     1921 ggcgcggcag gttcttcaat atggatatcg gcagcttcat cataaaccgg accgccgtat
     1981 cccacagacg aaacggtttc cgtgaaaaca ttttcggttt cagacggcat ttcgacatcc
     2041 cgtttcccga caaccgtccg actttccgga atcaggcgcg aaatccgcgc ctccgcagaa
     2101 accgttgcct tgttcggttc tttcaaattg acggaaacgc ggcggcaggc atcgcggacg
     2161 gctgtgccgt ctgaaacgga ctgcccttgg gaaacatccg gggattttgc ctccgcctgc
     2221 cctttcccct gccctgcatg acggtggaac ggcgcattcc gtgcattttc ggctttgact
     2281 gcagaaaagg cagacggatg ctgcttctca aaatacggct caaatccgta atccgcagac
     2341 tcggaaaacg cttctttgtc cgcatcgaac atatgggaat acgtgcgttg aagcacaggg
     2401 tcgtccaaac cgataatctg cagcccttcc atcggggtat cggacacgcg gactttgggg
     2461 accgctgttt cgtttttctc aaatgcatcg atatagcgtt ttttcgtttc ccttaaagcc
     2521 gcgctgttta aagcacgcgt tgcttcttcc aaagtgattt ctttaaaaac cggacggacg
     2581 ggcgaaattt cgctttcaga cggcatcaac ccttcagcaa tactccggtc gaatgggatg
     2641 cggcggttgt cgattacggc agttgcaatg tcttccgtat cggcagcctc ttcctccgca
     2701 gcttctgctt cttccgtttc cgcttcttcc gtcccgtttc cactgtctga atatccgtcg
     2761 gatgcggtcg gaacatccgc acggtttgca gaagcaggcg gaacgggcct gtccggttcg
     2821 gattcgtgcg ccagatagtt tcggcagaaa cggacgacac cgacaaacag ccaatacact
     2881 gccgttttga cggcatggaa aagcatcagg gcaagttcgg gaaaatcggg cataccgtct
     2941 ttgatttcag gcagctccgc ctgtttttcc cctttctttt cctgccacgc agaaacctcg
     3001 cgcatccact cgcgttcgga ttgtgcgcgg acaaaaaaca agccggcaag cgcaagcaac
     3061 aaaataacga tcataactat ccaaaacata tgccgtttcc cgcaagacaa acgattaaac
     3121 gggtttattt taacgtgttt ccatcccaaa aaaaacaacc ggtcggcaat tctgccgaaa
     3181 ccgattcggc tttcggtttg cccccgccga cagtataata gccgtccttt cccttaatct
     3241 ttgcggcatt gccatgattt tccaaacagt ctggttttca gatatggtac tgtcggtttc
     3301 gtggattgtc cttatcctga tactggcagc ttccgcgccg tccgcattcc gctcgcttga
     3361 ccgctaccgt tccgcccttc cactgtgtac cgtaattttt tccgcagcat ggtgtctgaa
     3421 cgcctctgcc ggcggcggac agcttgcaca gatgaactac cacctgctcg ccgtcaatct
     3481 ggttgcactg atggtgggca cttccgccgc cctttggctt gccgcgctgc tgatgctgcc
     3541 ctactgcctg ctgtttgccg gttctgtcgg cgcatatccg ccgaacgctt tggtgctgat
     3601 tctgcccgcg ctggtcgtca accgcctgtc gcgtatgctg gtcaaccgcc tgccgcccaa
     3661 tattttcatc tttattttcg tcaacggctt tctcgcttcc gccgccggca ttctgctgac
     3721 cgggctggtg ctgaccggta ttttggatgc cgcaaacgct tttccgtccg aaatattgtg
     3781 gacgaccgcc ctgcccgtct ttattctgct ggcgtgggcg gaagccttcc tcagcggcat
     3841 ttcaactgcc atatttgttg cactgaagcc ccattggatc aacactttcg acgacaaccg
     3901 ctatctgaag tccgaccgta gcatatggag gtagccttca gacggcatag gcagacaaaa
     3961 ccatgctttc caacatcatc cccctttcca tcggtgcggc actcggtgca acggcgcgtt
     4021 ggctgctcaa cctcgccgtt cctgccgcca tgtctcccgc cacgggcaac ctgtttgcca
     4081 actggacggg cgcgctgctc atcggcatct ttgccgaaac cgtcaatcac ccgcaatgga
     4141 agctgctgct gattaccggt tttctcggca gcctgaccac gctttccgga ttttcactgg
     4201 aaaccgtaac cctgctccaa tcgaatcgtc ccgcttcagc actttccaat attttcctac
     4261 acacggcagg ttcgctgctg ctgacttggc tcgggctgaa gatagggacg gcagtcaaat
     4321 aatcaaaaaa attgcttcct ttggttttga cacaaactat acaagtccta acatcttaat
     4381 catggcgcag catataagcg tggatttgca gcccaatcta tactttctga ttctattcat
     4441 taagccatta tgcttattcc tcaatcacgg tttccaccac tgaatgaata cttttaggca
     4501 attgcaaaaa agcattccaa gcaattaaag tagttttaaa agtatgcagc gaacagctta
     4561 aatcaggcca gccgtcttct tcatcaaatt cgttgttata gaaaactact ttttcagcag
     4621 aaatatccca gcaaaaacct tgtccacctc ctgaataaac ttcattggga ttattttctg
     4681 cttgatggat aaattctaaa ttttcatcta tgtctttttg tagataaagg tctcctgtta
     4741 aaacataaat taagtattca tatattctat ctttaattat ttcattattt tcttcatttt
     4801 cttcatctaa aaatatacat ctttttaata gttccccatt aaattcataa acatcgtaat
     4861 aagctttaaa aatagatatc atatcatttc cttatcttcc ataaatcggc ttcgcttgtt
     4921 taaatactgc ctgcgccaat taccgttctc aaattcaaat tgggctttta ggtctataac
     4981 aaagaaaaag ccttggcagg ataaacctac caaggctttt tattaaaaaa aacgctctat
     5041 tactgtgtct aaaaatgcaa aaaaagtaac catcataacc cttttgccaa aatcaatatc
     5101 atcatccgat gaatgaattg acggtatata agaaagaatt aataagctca taaaccggta
     5161 agaaaaatca gtaacaaaga tattcattat taactattct taaaaatgat cacgttcgtt
     5221 tgggtaatta tcagcccata ccatacccta ctcctattac taaagcacga atgactttaa
     5281 agggcgcacc cgacggcaag ccgaaccctt gcctccccga agccgaaatc agaaaaacca
     5341 taccccatcc gccaaacaaa atgcccgcca atctactccg gcgggcagtt caacaccctg
     5401 tcaatccttc atcagccagt attgcaaacc gattaatgtc ttgccgtctt taatttcatc
     5461 gtttgccaat gcctgacgga cttcttcttt cgacatcaat acggtttccg taatctcgtc
     5521 ttcgtcattg gcaagcgtac tgcccaaacg cacgccttcc gcctcgaaca gatacatttt
     5581 ttcgttgcaa aaaccgaccg ccgtataaaa actgtaaagc aggcgtacgc tgtcggcggt
     5641 ataaggcgtt tcttcagcca gttctcgtaa agcgcatgct gccatatcct cgctcgccac
     5701 atccagcttg cccgcaggaa gttccaatgt cgcctgattt gccgcataac gccactgccg
     5761 caccaaaacc actttcccct cgtccgtaac cgccaacacg catgccgcac ccggatggcg
     5821 gatgacgata cgctgccctt cattgccgtt gggcaacctg accttatccc tgctgatgct
     5881 gacgaaaccg ccctcgtaaa tggtttcgcc gcctaatttt acttccctca aatccacttc
     5941 atatctcctt tccgtttcct attctgccgt tcaatcttca cgcttgccgc gctcgatttc
     6001 cacgccaacc tcgcgcacgc cttccaaaat acccggcttg acgattttca cgcgcaccca
     6061 caccgcgccg aaatatccca aaaccaaatc ggcaatatat tccgccaacg cttccaaaag
     6121 caggaaatcc tgttctttca gatgtcggcg cagcgtttcg catacctcgg cataatgcac
     6181 cgtattggca atatcgtcgt ccgaacccgc tttctcggga acaccgatgt ccaaatcgac
     6241 aatcagggtc tgcaaccgtt cgcgctccca gccgtacacg ccgataagcg tatccgcctt
     6301 cataccgtgc aaaaagattt tatccataat ccgccccgtt ttttgctaaa ataaccgcac
     6361 cattctaagt aaagtaccgc aaatgttcaa catatcggct gttgctgttt cctatctgat
     6421 cggctcactt tctttcgccg tcatcgtgtc caaatattac ggcatggacg acccgcgcac
     6481 ctacggatcg ggcaatccgg gggcaaccaa tgttttacgc agcggcaaaa aaaaggcggc
     6541 cgcgctgacg ctcttgggcg atgccgccaa aggtttagtt gccgttttgc ttgcacgcgt
     6601 gcttcaagaa ccgctcggtt tatccgacag cgcaatcgcg gccgtcgcac tcgccgcgct
     6661 ggtcgggcat atgtggccgg tgtttttcgg atttaaaggc ggcaaaggcg tggcaacggc
     6721 attgggcgtg cttctggcac tctctcctgc aactgccttg gtctgcgcgt tgatttggct
     6781 tgtgatggca ttcggcttca aagtatcctc ccttgccgca ttaaccgcca caatcgccgc
     6841 cccccttgcc gcactgtttt ttatgccgca tacttcttgg atttgggcga cactccttat
     6901 cgccctgctg gtgttgttcc gccacaaaag taatatcgtc aagctgctcg aaggcagaga
     6961 aagcaaaatc ggcggcagcc gctgatttcc catccatccc tgtttatgcc gtctgaacca
     7021 cgcttcagac ggcatccgaa tatcccgccg ttttaccgac tatgctcagt tacagacacg
     7081 cattccacgc cggcaaccac gccgatatgc tcaaacactt taccttgttt ttagtgctgc
     7141 aatatttcaa ccgcaaagac aagccctact ggtacatcga cacgcacggc ggcgcgggtg
     7201 tgtacaatct cgaaggcagc aaagcacaga aagtcggtga ataccgccaa ggcatcgccc
     7261 tgctccgaca ggcgcaaaac ctgcctgccg aactctctga ctttgccgcg cacatacaaa
     7321 aaatcctgcc ctcgcccaaa ctttactgcg gttctccgtg gctggcgcaa tcgctgaccc
     7381 gtgtcggcga caaattgcgc ctgtttgagc tgcaccccac cgattttgtc catctgaaaa
     7441 acaatatggg cgaagcagga ttgggcaaac gtggacaagt attgcgcgaa gacggttaca
     7501 aagggctgat ttccctgctg ccgcccccgc cgcgccgcgc caccgtcctc atcgacccgc
     7561 cctatgagga aaaacaggac taccggcgtg taacggaaac gctgaaagcc gctttaaaac
     7621 ggtttgagtc cggctgttat ctcatttggt atccctgcct cagccgcgaa gaaagccgca
     7681 agctgcccga agaattgaaa aaactcctgc ccgacaacta ccttcacgcc gaacttcacg
     7741 tccacgcgcc gaaagccgac ggtttcggta tgcacggcag cggaatgttc gtcatcaatc
     7801 cgccctatct gttggcggaa caattggcgg caaacctccc cgccctgacg cggctgctgg
     7861 cgcaagacga aggcgcgcgc taccttttgg acagcaaaat ccgctgaccc cgatgccgtc
     7921 tgaaaccctt cagacaacat ctcccgtcaa ataaacaaaa ccggacgata taccggcagt
     7981 cggcactttt aagaataaat atcccctcat ccgcctgccg cgttccgact tgccccatgc
     8041 cgttttttta cactacaatc ccctagacta tcctgcaaaa cgcaccaaag gaatacgcca
     8101 caataacctg ccgcctttca gacggcacaa acctttccga aacgagcaaa ctatggacac
     8161 actgacccga ttcctccccg aacacctgca acaaaaccag ctgcccgaag cactcggcgg
     8221 cgttttactg tccgttgttt ccgcctgcac cgaaatcaac gccaaagtcc gcctcggcgc
     8281 actggccggc gtattgggta tggcgggcac gggcaatatt cagggcgaag accaaaaaaa
     8341 actggatgtt atcgccaaca acatcatgat tgacacactc aaagccaacc ctgccgttgc
     8401 cggtttggca agcgaggaag aagacacttt cgtaagtgcc ggtgaaaacg gacgctatct
     8461 cgtcctattc gaccctttag acggatcgtc caatattgat gtcaacattt ccgtcggtac
     8521 gattttctcc atccttgaaa aacccgaagg cgcattggca accgaatcat tccttcaaac
     8581 gggtagacag cagcttgccg caggctatgt cctctacggg cctcaaaccc agctcgtatt
     8641 cacattcgga cacggcgtat atatgttcac gctcaatgcc gaaaacgaat ttgtgctgac
     8701 aaaagaaaaa ccgaaagtac ccgaaagtac caaagaattt gccatcaata tgtccaaccg
     8761 ccgccactgg ctgccccccg tccaacaata catcgacgag ctcttggcgg gcgaaaccgg
     8821 cacgcgcggc aaaaactaca atatgcgctg ggtagccagc atggttgccg aaatccaccg
     8881 catcctgatg cgcggcggcg tgttcatgta tccgcaagac aaacgcgacc ccgccaagcc
     8941 cggcaaactg cgcctgatgt acgaagccaa ccctatgagc ctgatactcg aacaggcggg
     9001 cgcatcggca agcaacgcat atcaagccat gctcgacata cagcccgaaa acctgcacca
     9061 acgcgtcgcc gtcttcatgg gcagcagcga agaagtggat tacctcaacc gcctgcattc
     9121 aaaataaaag gcagcctgac cgataaagaa aggcggcagc gcgcatccga cgctgccgcc
     9181 gatatttcgt ttatagcctt aaaaagatag aaaaccacct ttccgaacgg caaaaatgcc
     9241 gtctgaaaag ttttcagacg gcatttcaaa aactcaaacc cgaattattt tcccgctgct
     9301 ttttgcagga tggcgtacaa ctcgtctttc agtttcaatt tggctttttt cagctcatcg
     9361 atggtttccg caccgctggt aaccggattg ttgaccagac cagtaatttt atcgtccagc
     9421 tcgttgtgtt cgtcgaacag acgggcgaag cgggaatttt cctgtttcaa tttggaaatc
     9481 aaatcacggt attctgggaa catatcgctt tcctctcttg gtttacggtt aaaaagtcaa
     9541 attgaatctt tacattaatt ataacaaaga taatattttt atccagtgtt ttatcggata
     9601 cggcaaaatt ttcttttaac accgccgtcc ggttgataaa aacgttgaca atatcacgct
     9661 ttacgcataa attttcagcc tttccta
//
LOCUS       DAWFHY010000075         9679 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.075, whole genome shotgun sequence.
ACCESSION   DAWFHY010000075 DAWFHY010000000
VERSION     DAWFHY010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9679
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.075"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            102..1286
                     /gene="coaBC"
                     /locus_tag="WGQ68_001636"
     CDS             102..1286
                     /gene="coaBC"
                     /locus_tag="WGQ68_001636"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEZ0715405.1"
                     /translation="MGKHILLGVTGSIAAYKSCELVRLLKKQGHSVTVVMSRSATEFV
                     SPLTFQALSGNPVLTDTHGGNGSNGMEHINLTRNADVFLIAPASMNTVAKICNGVADN
                     LLTNLAAARKCPLAIAPAMNVEMWLNPANQRNIAQLVSDGITVYMPGLGEQACGENGM
                     GRMPEPAELLDLLPDLWTPKILKGKKVLITAGATFEAIDPVRGITNISSGKMGVALAR
                     ACRAAGAEVSLIHGQLQTALPFGISDTVQAVSAENMHRAVHRLIDKQDAFISVAAVSD
                     YKVKNRSTQKFKKDKNAKRLSIELDENPDILASIASLPNPPFCIGFAAETENVMAYAR
                     EKRIKKKIPVIVANDVSIAMGKPTNRITIIGDDGELSFPETSKDEAAMQIVERLAVYL
                     NK"
     gene            complement(1353..3509)
                     /locus_tag="WGQ68_001637"
     CDS             complement(1353..3509)
                     /locus_tag="WGQ68_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEZ0715406.1"
                     /translation="MPVPQPTAPYDPLTAEARTLLFHTASYLKPEEQAELEKAVAYAF
                     RAHDGQTRKSGEPYITHPIAVATQLALWHMDIQGLCAGVMHDVLEDTGVTKGEMAEVF
                     GNTIAEMVDGLSKLEKLKFEDHAEHQAESFRKLILAMTKDVRVIVVKLADRLHNMRTL
                     GSMRPDKRRRIARETLEIYAQIANRIGLNNAYQELQDLSFQNLHPNRYETLKKAMDKS
                     RKNRQDVVGKVLRAFGQRLVGANIEAKIKGREKNLYGIHQKMMAKKLRFAEVMDIYGF
                     RVIVNSIPACYAALGALHTLYQPKPGRFKDYIAIPKSNGYQSLHTTLVGPYGLPIEVQ
                     IRTKEMDAVAEGGIAGHWSYKSYSKTVDQAVLHTNRWLKNILDLQASSANAIEFLEHV
                     KVDLFPNEIYILTPKGKILTLPKGATPVDFAYAVHTDIGHKTVAARINNIMMPLRTKL
                     KTGDSVEIITSEHAKPNPAWLNFAVSGRARSAIRQYIKNLNRHDAVVLGESLLQKALS
                     SLLPKDVLLSDGIKEKYLADLNDKQTSFEEVLYNVGMGHTLPVYVAMHIAELAGEHFG
                     SEVRLSSIKVDGQESGHIHFAECCHPVPGDSIRLLLVKGKGMIIHRDSCPTLLKSDPE
                     QQLDADWENMNGQNYRVGLQVQSEDSHGLLALMAQAISDSGADIESVETPSKSQSGTE
                     GFVEFKFLLKVKNLNQLNQIIQNLHSIPYIRKVIRS"
     gene            complement(3602..3808)
                     /locus_tag="WGQ68_001638"
     CDS             complement(3602..3808)
                     /locus_tag="WGQ68_001638"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEZ0715407.1"
                     /translation="MARITTEDCTGKISNHFDLTLVAARRARQLENGNTPLVDDARNN
                     KPTVTALREIAAGHIGTELLTRNK"
     gene            complement(3867..4484)
                     /gene="gmk"
                     /locus_tag="WGQ68_001639"
     CDS             complement(3867..4484)
                     /gene="gmk"
                     /locus_tag="WGQ68_001639"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEZ0715408.1"
                     /translation="MSAYRKGNIFIISAASGTGKTTLVSRLLANHNGLRVSVSHTTRP
                     PREGEVNGVHYHFVSKEEFESLIAQEAFLEYADVFGNYYGTSAEGVNALAAAGYDVIL
                     EIDVQGAAQVRDALPEAVGIFILPPSFDVLAARLNGRGTDSREVIQRRLSKARHEIEQ
                     SVLFDFVVVNDDLARAEEDLRHIVNACRLKRSRQLGFIADLLENS"
     gene            4612..5211
                     /locus_tag="WGQ68_001640"
     CDS             4612..5211
                     /locus_tag="WGQ68_001640"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEZ0715409.1"
                     /translation="MALKTSNLEHAMLVHPEAMSVGALADKIRKIENWPQKGILFHDI
                     TPVLQSAEYFRLLVDLLVYRYMDQKIDIVAGLDARGFIIGAALAYQLNVGFVPIRKKG
                     KLPFETVSQSYALEYGEAAVEIHTDAVKPGSRVLLVDDLVATGGTMLAGLELIRKLGG
                     EIVEAAAILEFTDLQGGKNIRASGAPLFTLLQNEGCMKG"
     gene            complement(5257..6045)
                     /locus_tag="WGQ68_001641"
     CDS             complement(5257..6045)
                     /locus_tag="WGQ68_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEZ0715410.1"
                     /translation="MNPKIVFFDIDDTLYRKYTDTLRPSVKTAVAALRGKGILTALAT
                     GRSLATIPEKVRDMMAETGMDAVVTINGQFALLHGKTVREVPMDAGLMGRVCAHLDGL
                     GMDYAFVGGEGIAVSALSECVCRALKHIASDFFADKDYFSSKPVYQMLVFAEENEMPL
                     WSDIVEQEGLKTVRWHEEAVDLLPAGASKTDGIRSVVEALGWEMADVMAFGDGLNDVE
                     MLSEVGFGVAMGNGEQAAKEAAKYVCPSVDEDGVLRGLQDLGVI"
     gene            6370..7725
                     /gene="yegQ"
                     /locus_tag="WGQ68_001642"
     CDS             6370..7725
                     /gene="yegQ"
                     /locus_tag="WGQ68_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HEZ0715411.1"
                     /translation="MKAPELLLPAGGLERMRTAYDYGADAVYAGSPRYSLRARNNEFA
                     KLDVLEQGIKEAHERNKKFFLTVNTLPHNSKLKTFVSDMEPLIAMKPDALIMADPGLI
                     MTVREKWPEMPIHLSVQANTTNYWGVKFWQNIGVERIILSRELSMEEIAEIRQECPDI
                     ELEVFIHGALCIAYSGRCLLSGYFNHRDPNQGTCTNSCRWDYKVHNATESDAGDAQLL
                     QGFNFEKAQEEANQNFEGINGQKRHPYADKVFLIEESNRPGEMMPIMEDEHGTYIMNS
                     KDLRGIEVVEKLAKIGVDSLKVEGRTKSLYYVARVAQSYRKAIDDAVAGRPFDYSLLS
                     ELEGLANRGYTSGFLERHQTQDYQNYLSGHSTAKQSQYVGHVTEIDENGWATIEVKNR
                     FAVGDSLEIIHPSGNQTIKLEQMARKGQPVDVAPGNGIQVKIPDMQGKEKALIARVLN
                     P"
     gene            7859..8335
                     /locus_tag="WGQ68_001643"
     CDS             7859..8335
                     /locus_tag="WGQ68_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease"
                     /protein_id="HEZ0715412.1"
                     /translation="MLAACQSGSAPKNAHTASADTPAGESCKVIRDDTAGSGKNIIYR
                     CCNGQAAINAAIADGVLESGIPVSFGGGGVPIANGRNLVTRQAANGVGKSDSEACEHA
                     LINAARKFQQTAGKLGGRSVTGFHSYFDKQPLQGGQYDCQTGSFHVRVVMRANVVR"
     gene            complement(8940..9326)
                     /locus_tag="WGQ68_001644"
     CDS             complement(8940..9326)
                     /locus_tag="WGQ68_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEZ0715413.1"
                     /translation="MRKILLDTHALLWWLLDDKKLGISARKLIENPRNAIFVSAASIW
                     EISIKQNKGLLKLPEEFFDVLQEEDFEMLPIGLFHAKQAGSLPEIHKDPFDRMLIAQT
                     QAEGFELMTVDEYIPQYGIRVVNASS"
     gene            complement(9323..9571)
                     /locus_tag="WGQ68_001645"
     CDS             complement(9323..9571)
                     /locus_tag="WGQ68_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEZ0715414.1"
                     /translation="MFQANIHQAKTNLSQLIQRAEAGEIVIIAKAGKPCVQLIGIEKP
                     ARNAGRLKKFSHMENTDISRILEDDNETAALFFEESAL"
BASE COUNT         2468 a         2594 c         2247 g         2370 t
ORIGIN      
        1 ggggcttcag acggcatttt gtatccaaaa cggtaactaa tgtatccgta ctttgttata
       61 gaatggctgc tgttttttct tcgtaattag aaattgtcaa aatgggcaaa catattcttt
      121 taggtgtaac gggcagtatt gcggcgtata agtcttgcga gttggttcgg ctgctgaaaa
      181 aacaggggca ttcggttacg gtggttatga gccgctcggc aactgaattt gtttctccgc
      241 tgacttttca ggctttaagc ggcaatcctg tcctgaccga cacgcacggc ggcaacggtt
      301 caaacggtat ggaacatatc aacctgaccc ggaatgcgga tgtttttctg attgcgccgg
      361 caagtatgaa taccgtggca aaaatctgta acggcgtggc agataaccta ctgaccaatc
      421 tggcagccgc acggaaatgt cctcttgcca tcgctcccgc gatgaatgtg gaaatgtggc
      481 tgaatcctgc caaccaacgg aatatcgcac aactggtttc agacggcatt actgtctata
      541 tgccgggctt gggcgaacag gcttgcggag aaaatggtat gggaaggatg ccggaacctg
      601 ccgaattgct ggatctgctt ccggatttat ggacaccgaa aattttaaag ggcaaaaaag
      661 tcttgattac cgcaggtgcg acatttgaag ccattgaccc tgtccgaggc atcacaaata
      721 tctccagcgg gaaaatgggc gtggctttgg cgcgggcgtg ccgtgccgcc ggtgcggaag
      781 tcagcctgat tcacggacag cttcaaaccg cgctgccttt cggcatatcc gatacggttc
      841 aagccgtcag tgccgaaaat atgcatcgcg cagtgcatcg tttaatcgac aaacaagatg
      901 cttttatttc tgttgccgcc gtctcagact ataaggttaa aaacaggagt acgcaaaaat
      961 tcaaaaaaga taaaaatgcc aaacggctat ccatcgaatt ggatgagaac cccgatattt
     1021 tggcttctat tgcctcattg ccgaacccgc cgttctgcat cggttttgcc gctgaaacgg
     1081 agaatgtaat ggcatatgca cgagaaaaac gtattaagaa aaaaataccg gtaatagttg
     1141 ccaatgatgt ttcaatcgcg atgggcaaac cgaccaaccg gattaccatt atcggggacg
     1201 acggggaact atcttttccc gaaacaagta aagatgaagc ggcgatgcag attgttgaaa
     1261 ggcttgccgt atatttgaac aaataagcaa ttgaacggat aaaccataaa gcgggttgcc
     1321 tgttaatcaa aaggcaaccc gctttacctg cttcaacttc tgatgacttt gcggatatat
     1381 ggaatactat gcagattttg aataatctga ttcaattgat tcagattctt gactttcaat
     1441 aagaatttga attcgacaaa accttccgtt cccgactggg atttagacgg tgtttcgacc
     1501 gactcaatgt ccgcaccgga atcggaaatc gcttgcgcca ttaatgccag caagccgtgg
     1561 ctgtcttccg attggacttg aagcccgaca cggtagttct gcccgttcat attttcccag
     1621 tctgcatcca gctgctgttc gggatcggac ttcaacaacg tcgggcagct atccctatgg
     1681 ataatcatgc cttttccctt aaccaacagc aaacggatgg aatcgccggg aacagggtgg
     1741 cagcactctg caaaatgaat atgcccgctt tcctgaccat cgactttaat ggaactgagc
     1801 ctaacctcgc tgccgaaatg ctcccctgcc aactcagcaa tatgcatggc gacataaaca
     1861 ggcagggtat gccccatacc gacgttgtac agcacttcct caaatgaagt ctgtttgtcg
     1921 ttgagatcgg caaggtattt ttctttgatg ccgtctgaaa gcaggacatc tttgggcagc
     1981 aaactggaca gggctttttg taagaggctc tctcccaaaa cgaccgcatc gtgccggtta
     2041 aggtttttaa tatattggcg tatggcactg cgcgccctgc cggatacggc gaaattcaac
     2101 cacgcgggat tgggtttggc gtgttcggat gtaataattt cgacggaatc gccggttttg
     2161 agcttcgtac gcaacggcat catgatattg ttgatacgtg cggcaacggt tttgtgcccg
     2221 atatcggtat gcaccgcata agcaaaatcg acaggcgttg cccctttggg caaagttagg
     2281 atttttcctt ttggcgtaag gatgtagatt tcgttcggaa acaaatcgac tttgacgtgt
     2341 tcgagaaact caatagcatt ggcactgctt gcctgcaaat ctaagatatt tttcagccac
     2401 cggtttgtgt gaagcaccgc ctgatcgacc gtcttagaat atgatttata gctccaatgt
     2461 ccggcgattc caccttcggc aacagcatcc atttccttgg tacgtatctg aacttcaatc
     2521 ggcaagccgt aagggccgac caaagtcgta tgcagacttt gatacccatt gcttttcgga
     2581 atggcgatat agtctttgaa ccgcccgggc ttgggctgat agagggtgtg caatgcgccg
     2641 agtgcggcat aacaggctgg aatgctgttg acaatgacgc ggaaaccgta aatatccata
     2701 acctcggcaa agcgcagctt tttcgccatc attttctgat ggatgccgta caggtttttt
     2761 tccctgcctt tgattttggc ctctatattc gcgcctacca gccgctggcc gaatgcgcgc
     2821 aagactttgc cgacaacgtc ctgccggttc ttccggctct tgtccatcgc tttttttaaa
     2881 gtctcgtagc ggttgggatg caggttttgg aacgataaat cctgaagctc ttgatatgcg
     2941 ttattcaaac ctatacggtt ggcaatctgt gcgtagattt caagggtttc ccttgcaata
     3001 cggcggcgtt tgtccgggcg catcgaaccg agtgtccgca tattatgcag acggtcggca
     3061 agtttaacga caatcacgcg cacatctttg gtcattgcca aaatcagttt gcggaaactc
     3121 tccgcctgat gttccgcatg atcttcaaat ttgagttttt cgagtttgga cagaccgtcc
     3181 accatctcgg caatcgtatt gccgaacacc tccgccattt ccccttttgt cacgcccgta
     3241 tcttccaata cgtcgtgcat cacgcctgca cacagaccct gtatgtccat atgccaaagg
     3301 gcgagctgcg tcgcaacggc aattggatgc gtaatgtatg gttcgccact tttccgggtt
     3361 tgtccgtcgt gggcgcgaaa cgcataggcg acggcttttt caagctccgc ctgttcctcg
     3421 ggcttgaggt aggaggctgt atggaaaagc agggtacgcg cttcggcggt cagagggtcg
     3481 taaggtgcgg tgggttgagg gacaggcatt tcagacggct ttcggaacat attttatttt
     3541 tcgtttcaaa ccgtcggact gcacggcggc agaatgtgcc gacgtgcgtt tccggcagaa
     3601 tttatttatt gcgcgtcaac agttccgtac cgatatgccc ggcggcgatt tcccttaagg
     3661 cggtaacggt cggtttgtta ttgcgggcat cgtccacaag cggcgtgttg ccgttctcaa
     3721 gctggcgggc gcggcgggcc gctaccaatg tcaggtcaaa atggttggaa atttttccgg
     3781 tacagtcttc ggtggtaata cgtgccatat tatttgcttt ctttcaaaaa tatttaaatt
     3841 gggaaactgg gtattttcgc ggttttttag gaattttcca acaaatctgc aataaatccc
     3901 agttgccgcg accttttcag acggcaggca ttcacaatat ggcgcaaatc ctcctccgcc
     3961 cgcgccaagt cgtcattgac cacgacaaag tcgaacaata cggactgttc gatttcatgc
     4021 cttgccttcg acagcctcct ttggataact tcccgactgt ccgtcccgcg cccgttgagg
     4081 cgcgcggcaa gtacgtcgaa agaaggcggc aggataaaga tgccgacggc ttcgggcagc
     4141 gcatctcgaa cctgagccgc accctgaacg tcgatttcca aaatcacgtc atagcctgcc
     4201 gccgccaacg cattcacgcc ctccgcgctt gtgccgtaat agttgccgaa cacatcggcg
     4261 tattccaaaa aagcttcctg cgcgataagc gactcaaact cttctttgga gacaaagtga
     4321 taatgcacgc cgtttacttc gccttcacgc ggcggacgcg tcgtgtgcga cacggaaacg
     4381 cgcaaaccgt tatgatttgc caacagccgc gacaccagcg tggttttgcc cgtgccggaa
     4441 gcggccgaaa tgataaagat gttgcctttt cgataagcgg acatattttt tacctgtata
     4501 ttttccagcc gattgtatca caacgaacac ccgatttcct atttgccgat gcccatattt
     4561 tgccgctatt gttttgattg atttggcaag cggcaggctg acggctacaa tatggcgtta
     4621 aaaacatcaa acttggaaca cgcaatgctg gttcatcccg aagctatgag tgtcggcgcg
     4681 cttgccgaca aaatccgcaa aatcgaaaac tggccgcaaa aaggcatctt attccacgac
     4741 atcacgcccg tcctgcaaag cgcggaatac ttccgccttt tggtcgattt attggtttac
     4801 cgctatatgg atcagaaaat cgacatcgtt gccggtttgg acgcacgcgg cttcattatc
     4861 ggcgcggcac tcgcctacca gctcaatgtc ggtttcgtcc ccatccgcaa aaaaggcaag
     4921 ctgccttttg aaaccgtatc gcaaagctac gcgctcgaat acggggaagc tgcggtggaa
     4981 atccacaccg atgccgtcaa acccggttcg cgcgtgctgc tggtcgatga tttggttgcc
     5041 acgggcggca cgatgcttgc cggactggag ctgatccgca aactcggcgg ggaaattgtc
     5101 gaagctgccg ccattttgga atttaccgac cttcaaggcg gcaagaatat ccgtgcaagc
     5161 ggcgcgccct tatttaccct gcttcaaaac gaaggctgta tgaagggctg aaaaccgacc
     5221 ctgccgtctg aaaccggcag ggttgttatt atgcgttcaa atcacgccca aatcttgcaa
     5281 gcccctcaac acgccgtctt catcaacgct ggggcaaaca tatttcgccg cttctttcgc
     5341 cgcctgttcc ccgttgccca ttgccacgcc gaacccgact tcagacagca tttccacatc
     5401 gttcaaacca tcgccgaacg ccatcacgtc tgccatttcc catcccaatg cttcaaccac
     5461 gcttctgatg ccgtctgttt tcgacgcgcc cgcaggcagc agatcgacag cttcctcatg
     5521 ccagcgcacc gttttcaagc cttcctgttc cacaatatcc gaccagagcg gcatttcgtt
     5581 ttcctccgca aacaccagca tctgatacac cggtttgctt gagaaataat ccttatcggc
     5641 aaaaaaatcg ctggcgatat gcttcaaggc gcggcacaca cattccgaca gcgcggacac
     5701 ggcgatcccc tctccgccga caaacgcata atccatgccc aagccatcca aatgcgcgca
     5761 aaccctgccc atcaaaccgg catccatcgg tacttcgcgc acagtttttc cgtgcagcag
     5821 cgcaaactgc ccgtttatcg ttaccacggc atccataccc gtttccgcca tcatgtccct
     5881 gaccttttcg ggaatcgtcg ccaaagaccg ccccgttgcc aacgccgtca atatgccttt
     5941 gccgcgcaaa gccgccaccg ccgttttcac ggaaggacgc aaggtatccg tatatttgcg
     6001 gtacagcgta tcgtcaatat cgaaaaacac gattttggga ttcatcatat tctctctcgc
     6061 actcaaactg ccgcattgta tcccaatcag gcaaatactt gataaatcct tataaatttc
     6121 ccggcaaaat tgaccgcgaa cacgaaaagg cggataatcc gcccatcctc aaaccctttt
     6181 cagacggcat ttgcagcaat gccgtctgaa acatttttac aaagcataca aatcatgttt
     6241 caacacacag gacgacacat aaagcgtcgc cctatatgtt gccctgattc ggaaggggtt
     6301 acgcccctcc caaataaagt ctgattctac cgccctaaag ggcggggttt caaccgaaaa
     6361 ggaaacacga tgaaagcacc cgaactctta ttgcccgccg gcggattgga aagaatgcgc
     6421 accgcctacg actacggcgc agatgccgtt tatgccggca gcccgcgtta ttccctgcgc
     6481 gcccgcaaca acgaatttgc caaactcgac gtcctcgagc aaggcattaa agaagcgcac
     6541 gagcgcaaca aaaaattctt tttgaccgtc aatacgctgc cgcacaactc caaactcaaa
     6601 accttcgttt ccgacatgga gccgctgatt gcgatgaaac ccgacgcgct gattatggcg
     6661 gatccgggtt tgatcatgac cgtgcgcgaa aaatggccgg aaatgccgat ccacctgtcc
     6721 gtacaggcga acaccaccaa ttattggggc gtaaaattct ggcagaacat cggtgtcgaa
     6781 cgcatcatcc tgtcgcgcga attgagtatg gaagaaatcg ccgaaatccg ccaagaatgc
     6841 cccgacatcg aactcgaagt cttcatccac ggcgcattgt gcatcgctta ttcaggccgt
     6901 tgcctattgt cgggttattt caaccaccgc gaccccaacc aaggcacttg caccaactcc
     6961 tgccgttggg actacaaagt ccacaacgcc acggaaagcg atgcgggcga tgcccagctt
     7021 ctgcaaggtt tcaactttga aaaagcccaa gaagaagcca accaaaactt tgaaggcatc
     7081 aacggtcaaa aacgccatcc ctacgccgac aaggttttcc tgattgaaga atccaaccgt
     7141 ccgggcgaga tgatgccgat tatggaagac gaacacggca cttacatcat gaactccaaa
     7201 gacctgcgcg gcattgaagt ggttgagaaa ctcgccaaaa tcggcgtgga cagcctcaaa
     7261 gtcgaaggcc gcaccaagtc gctctattat gtcgcacgcg tcgcccaatc ctaccgcaaa
     7321 gcgattgacg atgccgtcgc aggccgtccg tttgattaca gcctgttgag cgaactcgaa
     7381 ggccttgcca accgcggcta caccagcggc ttcctcgaac gccaccaaac ccaagactat
     7441 caaaactacc tcagcggcca ttccaccgcc aaacaaagcc aatatgtcgg ccacgttacc
     7501 gaaatcgacg aaaacggctg ggcgaccatc gaagttaaaa accgcttcgc cgtcggcgat
     7561 tcgctcgaaa tcatccaccc gagcggcaac caaaccatca aattggaaca aatggcccga
     7621 aaaggccagc ctgtcgatgt cgcacccggc aacggcattc aggtcaaaat ccccgatatg
     7681 cagggcaaag aaaaagccct catcgcacgc gtgttgaacc cctgagccac aatgccgtct
     7741 gaaccccgtt ttcagacggc attttgttga atcttacggt acaatagcaa tgatttccca
     7801 cagcaaacaa ggacatccga catgaaaaaa aatcctcata ctctccgccg ccgccctgat
     7861 gctggcagcc tgccaatccg gcagcgcacc taaaaacgcg cacacggcat ccgccgacac
     7921 accggccgga gaatcatgca aagtcatccg ggacgacacc gcaggaagcg gcaaaaacat
     7981 catttaccgt tgctgcaacg gtcaggcggc aataaatgca gccattgcag acggcgtatt
     8041 ggaatcaggg attcccgtca gcttcggcgg cgggggcgta ccgattgcaa acggcaggaa
     8101 tctggttaca cggcaagcag caaacggcgt tggcaaatcc gactccgagg cctgcgaaca
     8161 cgccctaatc aatgccgccc gcaaattcca gcagaccgca ggcaaactcg gcgggcgcag
     8221 tgttaccggc ttccacagct acttcgacaa gcagccgctg caaggcgggc aatacgactg
     8281 tcagacaggt tcgttccacg tccgcgtggt catgcgcgcc aatgtcgtcc gttaacatat
     8341 cggcaatcaa aaaattaggc tgtgttttag taatctgttg atttcaatta tttgcaaagg
     8401 aaaagacaat tattttttcg gttaggaaga gacctatcct attgaataca tttaagttaa
     8461 atacgtctat caacactata ttatagtgga ttaacaaaaa tcaggacaag gcgacgaagt
     8521 cgcagacagt acagatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc
     8581 gttctctttg agctaaggcg aggcaacgcc gtactggttt ttgttaatcc actataaaac
     8641 acagccatat atattaagta cctgctcttt atgcgctttt ttaggaaagg aagatcaaac
     8701 ggttcaatag cgaagtcttg cccaaatttg atggacagga tatagaatac atgcgtgcat
     8761 accacaccct gccaaagttg ggagatgcac ttaaggttgc acttgtggtc gccaactatg
     8821 cctatacacg cggatttccc gcgtttatag gccgcgacgg aagtctggct tttaagcgaa
     8881 gacaataacc gaaaagaccg cctcggcggt ctttcttgtg tacacagttt ttgtatttgt
     8941 cagcttgatg cgttgacaac tctaattcca tactgcggaa tatattcatc gacagtcatc
     9001 agttcaaagc cttccgcttg ggtttgtgca atcaacatcc tatcgaaagg gtctttgtgt
     9061 atctccggaa ggcttccagc ctgttttgca tgaaacagac ctataggcaa catttcaaaa
     9121 tcctcttctt gaagcacatc aaaaaactct tccggtaatt tcaacaaccc cttgttctgc
     9181 ttgatggaaa tttcccaaat acttgctgca ctgacaaaga tcgcatttct cggattttct
     9241 atcagtttgc gtgcagatat ccccagtttc ttgtcatcca acaaccacca cagcaacgca
     9301 tgggtatcaa gcagaatctt tctcacagag ccgactcctc aaaaaataaa gctgccgttt
     9361 cattgtcatc ctcaagaata cgtgaaatat ccgtattttc catatgactg aattttttca
     9421 accttcctgc atttcgtgcc ggtttttcaa taccgattag ttggacgcaa ggcttacctg
     9481 ccttcgcaat aataacaatt tcccctgctt ctgctctttg aatcaattga ctcaaattgg
     9541 tttttgcctg atgaatattt gcttgaaaca taacacttct catgattagc taacttaact
     9601 aatatacatc attaccaaga ttttgggaat ctcattacat atatttgatt atatccgccg
     9661 ttttattcac accttgcta
//
LOCUS       DAWFHY010000076         9599 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.076, whole genome shotgun sequence.
ACCESSION   DAWFHY010000076 DAWFHY010000000
VERSION     DAWFHY010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9599
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.076"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(1..1182)
                     /locus_tag="WGQ68_001646"
                     /pseudo
     CDS             complement(1..1182)
                     /locus_tag="WGQ68_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019271049.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            1328..2032
                     /locus_tag="WGQ68_001647"
     CDS             1328..2032
                     /locus_tag="WGQ68_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248833.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEZ0715415.1"
                     /translation="MLRSILAASLLAVSFPAAAEALNYNIVEFSESAGVEVAQDTMSA
                     RFQVAAEGRDKNAVNAEFVKKFNSFNRKLKNGSFKTELVSRSAMPRYQYTNGRRIQTG
                     WEERAEFKVEGRDFDALNRFIADVQTDASLEYTDFSVSRERRNEVIDQVSKDAILRFK
                     TRAEKLAGILGASGYKIVKLNLGHIGSHIAGGGAAQAKMLRAMPMAASVNMEGADSAA
                     QGVEEISISVNGTVQF"
     gene            complement(2096..2662)
                     /locus_tag="WGQ68_001648"
     CDS             complement(2096..2662)
                     /locus_tag="WGQ68_001648"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003823864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEZ0715416.1"
                     /translation="MSIKSDKWIRRMSEEFGMIDPFEPNQIKEADGKRIISYGTSSYG
                     YDIRCANEFKIFTNINSTIVDPKNFDPKNFVTVEDDCCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPFEPEWEGYVTLEFSNTTPLPAKIYAGEGVAQVLFF
                     ESDEICKTSYKDRNGKYMGQTGVTLPKA"
     gene            complement(2728..3144)
                     /locus_tag="WGQ68_001649"
     CDS             complement(2728..3144)
                     /locus_tag="WGQ68_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715417.1"
                     /translation="MLKHLAFLLPAMMFALPAASAVLTSYQEPGCTYEGDVGKDGKPA
                     GKGTWRCQDGRNYTGSFKNGKFDGQGVYTVAANREIFIEPFNSDSTKFRNMVLSGTFK
                     KGLAHGRFTVSQNGETLFIMKCENGMIKEVKLPKNK"
     gene            complement(3196..4095)
                     /locus_tag="WGQ68_001650"
     CDS             complement(3196..4095)
                     /locus_tag="WGQ68_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HEZ0715418.1"
                     /translation="MWFKQISFYPLNKEKLPEADALADKLAEAEFTHCQGLDWFSEGF
                     TAPVSFSPELVFPADFTLRVALKKEEKVLPAGVIRDILEEKVAEIQNNEARNVGRKEK
                     QELKEQITDDLLPRAFTRSSRTEAVFNTRHGYLLVNNAASAKAENILTKLREALGGLE
                     ASLPNTKQSPSSLMTGWLLQGHCEGGFELDSDCELKGTGDIVPVVKVSKQDLTADEVV
                     QHVKNGKTVTQLGLVWREQIAFILTQDFTLKRIQYLDVLQEEAESNGDDAAGLAFASQ
                     ILMAESVSIMLEELVSYLGGWQD"
     gene            complement(4126..5583)
                     /gene="der"
                     /locus_tag="WGQ68_001651"
     CDS             complement(4126..5583)
                     /gene="der"
                     /locus_tag="WGQ68_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003745900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEZ0715419.1"
                     /translation="MKPTIALVGRPNVGKSTLFNRLTRTKDALVHDLPGLTRDRHYGH
                     GKVGSKPYLVIDTGGFEPVVDSGILHEMAKQTLQAVDEADAVVFLVDGRTGLTPQDKI
                     IADRLRQSPRPVYLAVNKGEGGNRAVLAAEFYELALGDPYVISGAHGDGVYYLIEDIL
                     ETFPEPEAEEADAKHPVFAVIGRPNVGKSTLVNAILGEERVIAFDMAGTTRDSIHIDF
                     EREGKPFTIIDTAGVRRRGKVDEAVEKFSVIKAMQAVEAANVAVLVLDAQQDIADQDA
                     TIAGFALEAGRALVVAVNKWDGISEERREQVKRDINRKLYFLDFAKFHFISALKERGI
                     DGLFDSIQAAYNAAMIKMPTPKITRVLQSAIERQQPPRAGLVRPKMRYAHQGGMNPPV
                     IVVHGNSLHAISDSYTRYLTQTFRKAFNLQGTPLRIQYNVSENPYENAEDKPKKKPLR
                     RVSLSNRIEKREGRKEEKNRFKKKTKVSVKKQFSK"
     gene            complement(5737..6366)
                     /locus_tag="WGQ68_001652"
     CDS             complement(5737..6366)
                     /locus_tag="WGQ68_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgM family protein"
                     /protein_id="HEZ0715420.1"
                     /translation="MAAHLEEQQELDNFKYFWKTTGKWLFALLILAALGYLGYTVYQN
                     RATSQNQEAAAVLANIVEKAQNKAPQSEINAELTKLQQSYPHSISTAQATLMAAATEF
                     DAQRYDVAEGHLKWVLSNQKDSLIQALAAQRLGVVLLQQKKYDAALAALDTPVEADFA
                     PLLMETKGDVYAAQGKSQEALKNYGQALEKMPQDSVGRELVQMKLDSLK"
     gene            complement(6367..7662)
                     /gene="hisS"
                     /locus_tag="WGQ68_001653"
     CDS             complement(6367..7662)
                     /gene="hisS"
                     /locus_tag="WGQ68_001653"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEZ0715421.1"
                     /translation="MAQKIQSVKGMNDLLPVEQKDFKLTAAFWQAFEDTVGRWTRTYG
                     YQQIRTPIVEQTGLFVRSIGEETDVVGKEMYTFSDSNDSLSLSLRPEGTASCLRAVVE
                     HNLLYNSPQKLWYMGPMFRRERPQKGRYRQFHQVGIEALGFEGPDIDAEIIAMSADLW
                     EKLGIREYLTLEINSLGNREERAAHRAALVEYLTRYEDKLDEDSKRRLKTNPLRVLDT
                     KNPDLQEICNAAPRLVDYLGEASQNHYARFKAMLDGLGIQYIENPRLVRGLDYYNQTV
                     FEWTTDKLGAQATVCGGGRYDGLIEELGGKPAPSIGFAMGIERLLFLVSEYGSLEVNA
                     APDVYAMHQGERADLQVMKYAQALRTQGFNVMQHSGYQSLKAQMKKADNSGARFALIV
                     AQDELANGTVTLKDMNGAHGQQTVAAADLTNTLQQWKNA"
     gene            complement(7756..8298)
                     /locus_tag="WGQ68_001654"
     CDS             complement(7756..8298)
                     /locus_tag="WGQ68_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEZ0715422.1"
                     /translation="MQSWKERRDFNIVKQDLDFSCGAASVATLLNNFYGQTLTEEEVL
                     KKLDKEQMRASFEDMRRIMPDLGFEAKGYALSFEQLAQLKIPVIVYLKYRKDDHFSVL
                     RGIDGNTVLLADPSLGHVSMSRAQFLDAWQTREGNLAGKILAVVPKKAETISNKLFFT
                     HHPKRQTEFAVEQIRQARAE"
     gene            complement(8470..8661)
                     /locus_tag="WGQ68_001655"
     CDS             complement(8470..8661)
                     /locus_tag="WGQ68_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715423.1"
                     /translation="MLPTRSVSSLLLGEYLEFEENGTKIAAEIDSAWHFLGAACRISI
                     NGKYYAGNRIVWFAKKAES"
     gene            complement(8694..9230)
                     /locus_tag="WGQ68_001656"
     CDS             complement(8694..9230)
                     /locus_tag="WGQ68_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010359781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715424.1"
                     /translation="MEKIIDSKVRETNFNQAWRVIRAMQIDNISDEQVVIVRMSCYLE
                     KDEIFKNVERYGYQSSDIKKISPPFPLNILPVPKPYYFYLYDTHLNQIYDLAWSCNDF
                     LMFEIFILDRESIKYIDKDKLFKSGETLYSNFKSIKNQCKNYFFCGFDFDAPYFKSGV
                     SQVADYNFVPDAFSLFFI"
     gene            complement(9398..9598)
                     /locus_tag="WGQ68_001657"
     CDS             complement(9398..9598)
                     /locus_tag="WGQ68_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010981156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715425.1"
                     /translation="MQLICADWTGIGNFMKTFEKTWSAQYRDMEISVRNFWNLERTGA
                     EVYINGRRVYHNEAEMASASLR"
BASE COUNT         2255 a         2302 c         2434 g         2608 t
ORIGIN      
        1 ggtattttct taccgtattc tgcgattgcc tgtcggaaaa tgccgatcaa cctgcctata
       61 acggcatttt cgccaaattc gttcagacag ttttccctaa gtcgggcagg ttcaaaatca
      121 gagtggtgtt catacatttt gatgagtgcg tcggcaaggg catcgtcgtc gtcaacagga
      181 accaaatatc cgttgccgtc tgaaacaata gattccgcac cgccgcagcg tgtcgcaatg
      241 acgggcaatc cttgggacag tgcttcgata tagaccacgc cgaaggtttc ggtgcggctg
      301 gcaaggacaa atgcgtcgct gttcttcatc aaatccaaga ctgcttcggg ttgcagtgcg
      361 cctaaaaatg taacggcatg ggtaatgccc aagtctgccg cctgctgttt cagccgctgt
      421 tcttcctgtc cgctgccgcc gatgtttaga cgcagttgcg ggcattgtgc caacgcccgg
      481 gcaaaggcag tgagcaggac atcgtgtcct ttgagacggc gaaggtgcga gacggtgcag
      541 aacacgaaat gcggtttgtt gtttttttct ctttgttcaa acggtcggtt gaatattccg
      601 cccagtatgt tggggaggta ctgccattcg cagccgtatt tgtgttgcag gacgtgtgcg
      661 aaatggcggc tgatggcgag acgtgcggcg gcgtgcgccg ccgcattttt cataggctgc
      721 cattggtggg gacggatcag accgcgcgta atggtgctgc tgtgtttcgt gacgacatag
      781 gggatgccgt atttttggga aattttttgg gcgagtatgc cggcatagtt catgcggtgg
      841 gcgtgaatca ggtcgggcag cccgttttcg cggatgtagt gtttgaaggc tttcaaaccc
      901 acgcgcaccc agcggatgcg gtcgatgtcg atgaacggaa agcgggggaa gaaatacatg
      961 ccgtgccatg catagatgtc caaaccgctt tgccgatatt ttgcaaaacc gtaaggtcgg
     1021 tcaggatgct tgctgtttct ttccgcaagt agcggaacat cggtgcaagc acggcggttt
     1081 tgatgccttt cctctgcaat gccagtgcct gattttgaaa aaaatcccgt ccacatcctg
     1141 ttcggattgc ggataccatg aggggatgac gaggacgtgc aagggtttgg gcatagtggg
     1201 attccgtatc ggaaaggcgg ttattataag acagacgcag accgaatatt taaattgttg
     1261 ccttacgcta atgcaattta gcgcgccgat atgctagatt ggcggtttta tgagtgagga
     1321 tacggatatg ttgcgttcta ttttggcggc ttccctattg gcggtatctt ttccggcagc
     1381 ggctgaagca ttgaattaca atattgtcga attttccgaa tcggcgggtg tcgaggtggc
     1441 tcaggataca atgtccgcac gtttccaagt ggcggcggaa ggacgggaca aaaatgccgt
     1501 caatgccgag tttgttaaaa aattcaacag ttttaacaga aaattgaaaa acggcagctt
     1561 taaaaccgaa ttggtatcgc gcagtgcgat gccgcgctat caatatacca acggcagacg
     1621 cattcaaaca ggctgggagg agcgtgcgga atttaaggtc gaaggtaggg attttgatgc
     1681 gttgaaccgt tttattgctg atgttcagac ggatgcttcg cttgaatata ccgatttcag
     1741 cgtgtcgcgt gaacgccgaa acgaggtcat cgatcaggtc agcaaggatg ctattttgcg
     1801 ttttaagacg cgtgccgaaa aactggcggg cattttgggt gcgtccggtt ataaaatcgt
     1861 caaattaaat ttgggacaca tcggcagcca tatcgcggga gggggagctg ctcaggcaaa
     1921 aatgcttcgt gccatgccga tggcggcaag cgtcaatatg gagggtgcgg attctgccgc
     1981 gcagggtgtg gaggaaatca gcatcagcgt caatgggacg gttcagttct aaccacggat
     2041 aaacaggtag atgccgtctg aaaccggatg atacggttca gacggcattt atatttcagg
     2101 ctttaggcag ggtaacgccg gtttgcccca tatatttgcc gttgcggtct ttgtatgagg
     2161 ttttgcagat ttcgtcgctc tcgaagaaga ggacttgcgc cacgccttcg ccggcgtaga
     2221 ttttggcggg caggggggtg gtgttggaaa actcgagggt aacgtagcct tcccattccg
     2281 gctcgaacgg ggtaacgttg acgatgatgc cgcagcgggc gtaggtggat ttgcccaagc
     2341 agacggtcag gacgttgcgc gggatgcgga aatattcgac cgtgcgcgcc agtgcgaagg
     2401 aattgggcgg gatgatgcag cagtcgtctt caacggtaac gaagtttttc gggtcgaagt
     2461 ttttgggatc gacgatggtg ctgttgatgt tggtgaagat tttaaattcg tttgcgcagc
     2521 ggatgtcgta gccgtagctg gacgttccgt aggagatgat gcgtttgccg tcggcttctt
     2581 tgatttggtt cggctcgaaa gggtcgatca tgccgaattc ttcgctcatt cggcgtatcc
     2641 atttgtcgga cttgatgctc ataatgtttt ccttgtttct tgcagtgttc ggacaaagca
     2701 ttgggggatg ccgtctgaaa acggggctta tttgtttttg ggcagcttca cttctttaat
     2761 catgccgttt tcgcatttca taatgaagag ggtttcgccg ttttgcgaga cggtaaatct
     2821 gccgtgtgcc aagccttttt tgaatgtgcc cgagagtacc atgttgcgga atttggtact
     2881 gtcggaattg aacggttcga taaatatttc gcggttggcg gcaacggtat aaacgccttg
     2941 tccgtcgaat ttgccatttt taaacgaacc ggtatagttg cgcccgtctt ggcagcgcca
     3001 tgtgcctttg ccggcgggtt taccgtcttt gccgacatcg ccttcgtagg tgcagccggg
     3061 ttcttgatag gaagtcagaa cggcggacgc ggcggggagg gcgaacatca tggcgggcag
     3121 taggaatgcg agatgtttaa gcataagggt tattccattg gattttggct gacggtataa
     3181 aagccgtctg aaaaatcaat cttgccagcc gcccaaatag gaaaccagct cttccaacat
     3241 gatgctgacg gattccgcca tcaggatttg cgaggcgaag gcgagaccgg cggcatcgtc
     3301 gccgttgctt tcggcttctt cctgcaatac gtcgaggtat tggatgcgct tgagtgtgaa
     3361 gtcttgcgtg aggataaagg cgatttgttc gcgccaaacc aaaccgagct gggttacggt
     3421 tttaccgttt ttgacgtgtt gaaccacttc gtcggcggtt aaatcttgtt tggatacttt
     3481 gacgacggga acaatatcgc ccgtgccttt gagttcgcaa tcgctgtcca attcaaaacc
     3541 gccttcgcaa tgcccttgca acagccagcc ggtcatcagg gaagagggcg attgtttggt
     3601 attcggcagt gaggcttcca aaccgcccaa agcttcgcgc agcttggtca ggatgttttc
     3661 tgctttggcg gaagccgcgt tattgacgag caggtagccg tggcgggtgt taaacaccgc
     3721 ttctgtacgg ctgctgcggg taaacgctcg gggcagcagg tcgtctgtaa tttgctcttt
     3781 aagctcttgt ttttctttgc gaccgacatt gcgggcttca ttgttttgga tttccgctac
     3841 cttctcttcc aaaatatcgc ggatgacgcc ggcaggcagg actttttctt cttttttcag
     3901 ggcgacgcgc aaggtaaagt cggcagggaa aacgagttcc ggggagaatg aaaccggtgc
     3961 ggtaaagcct tcgctgaacc agtctaagcc ttggcaatgg gtaaattcag cttcagcaag
     4021 tttgtcggca agtgcgtccg cctcaggcag cttttctttg ttgagcggat aaaaactaat
     4081 ctgcttgaac cacataatgt ttcctattgt ttgaaatgtc gggaattatt tgctgaattg
     4141 ttttttcaca ctgactttgg ttttcttctt gaagcggttt ttctcttctt tgcggccttc
     4201 gcgtttctca atacggttgc tcaggctgac gcggcgcagc ggttttttct tcggtttgtc
     4261 ttccgcattt tcatacgggt tttccgaaac attgtattga atccgcaacg gcgtaccttg
     4321 cagattgaag gctttgcgga acgtttgggt caggtagcgc gtatagctgt cggaaatcgc
     4381 gtgcagcgaa ttgccgtgta ccacaatcac cggcggattc atgccgcctt ggtgggcata
     4441 acgcattttc ggacgcacca agccggcacg cggcggttgc tgacgttcga tcgcgctttg
     4501 cagtacgcgc gtgattttcg gcgtcggcat cttaatcatt gccgcgttgt aggcagcctg
     4561 aatgctgtcg aacaatccgt ctatgccgcg ttctttcaac gcggaaataa agtggaattt
     4621 agcaaaatca aggaaataca gtttgcggtt gatatcgcgt ttcacttgct cgcgacgttc
     4681 ttcgctgatg ccgtcccatt tattgaccgc caccaccaaa gcacggcctg cctccaaagc
     4741 gaaacctgca atcgtcgcat cttggtcggc gatgtcctgc tgcgcgtcca ataccaaaac
     4801 agcgacgttt gccgcttcaa ccgcctgcat cgctttgata acggagaatt tttctactgc
     4861 ttcatccact ttgccgcgac ggcgcacgcc tgcggtatcg atgatggtaa acggtttgcc
     4921 ttcgcgttcg aaatcgatgt ggatgctgtc gcgtgtcgtg cctgccatat cgaaagcaat
     4981 cacgcgctct tcgccgagga tggcgttaac cagtgtggat ttgccgacgt tcggacggcc
     5041 gatgacggca aagacgggat gttttgcatc ggcttcttcg gcttccggct ctgggaaggt
     5101 ttccaaaata tcttcaatca gataatacac gccatcgccg tgcgcacctg aaataacata
     5161 agggtcgccc aaagcaagtt cgtagaactc ggcggcaagc acggccctgt tgccgccttc
     5221 gcctttgttt accgccagat aaacagggcg cgggctttgg cgcaggcggt cggcaatgat
     5281 tttgtcttgc ggggtcaggc cggtacggcc gtccaccaaa aacacaaccg catcagcttc
     5341 atcgacagcc tgcaaggttt gttttgccat ttcgtgcaaa atgccgctgt ccacaaccgg
     5401 ctcgaaacct ccggtatcga tgaccaaata aggtttgctg ccgactttgc cgtgtccgta
     5461 atggcggtcg cgggtcaggc cgggcaggtc gtgtacgagt gcgtctttgg tgcgcgtcaa
     5521 acggttgaat aaagtagatt tgccgacgtt agggcggccg acaagcgcga tggtgggttt
     5581 cattattggg tctttctgtg tcaagtgccg ttcgggagaa ctgaacacga gcaggtgtcc
     5641 gttggacacg gtaaataggt tcttcacggc aagaggccat aaagagggag ggtaaaatgc
     5701 cgtctgataa ataatggaca gcatttcaga cggcatttat ttcagcgaat caagtttcat
     5761 ttgaaccaat tcgcgaccga cagaatcttg aggcattttt tctaaagcct gtccgtagtt
     5821 ttttaaggct tcctggcttt ttccctgtgc ggcatagaca tcgcctttgg tttccatcag
     5881 caggggggcg aagtccgctt caaccggcgt atcgagcgcg gcaagcgcgg catcgtattt
     5941 tttttgttgc aacaacacaa cgcccagacg ctgcgccgcc aacgcttgaa tcaggctgtc
     6001 tttttggttg gacaacaccc atttcaaatg gccttcggca acatcgtaac gctgtgcgtc
     6061 aaattcggtt gccgccgcca tcagcgtggc ttgggcggtg gaaatggaat gcgggtagct
     6121 ttgctggagt ttggtcaatt cggcattgat ttcgctttgc ggggctttgt tttgcgcctt
     6181 ttccacgatg tttgccagca ccgccgccgc ttcctgattt tgggaagtcg cacggttttg
     6241 gtaaaccgtg tatcccaagt agccgagcgc cgccaaaatc agcagggcaa acagccattt
     6301 gcccgtggtt ttccaaaaat atttaaagtt gtccaactct tgttgttctt ccaaatgggc
     6361 tgccatttat gcgttcttcc attgttgtaa agtgtttgtc aaatcagcgg cggcgacagt
     6421 ttgctgaccg tgtgcgccgt tcatatcttt gagcgtaacc gtaccgttcg ccagttcgtc
     6481 ttgcgcgaca atcagggcaa agcgcgcgcc gctgttgtcg gcttttttca tttgcgcttt
     6541 caggctttga tagccggaat gctgcattac attgaaacct tgtgtgcgca aggcttgcgc
     6601 gtatttcatc acttgcaagt ccgccctttc gccttggtgc attgcataga catcaggcgc
     6661 agcattcact tccagcgagc cgtattcgct caccaaaaac agcaaccgtt cgatgcccat
     6721 tgcaaagccg atagacggcg caggtttgcc gccaagttct tcaatcaaac catcgtaacg
     6781 gccgccgcca cacacagtcg cctgcgcgcc gagtttgtcg gtcgtccact caaaaaccgt
     6841 ctgattgtaa taatccaaac cgcgaaccaa gcgcggattt tcaatatatt gaatacccaa
     6901 accatccaac atcgccttga agcgtgcata gtggttttgc gaagcctcgc ccaagtaatc
     6961 caccaaacgc ggcgcggcat tgcagatttc ctgcaaatca gggtttttcg tatctaaaac
     7021 gcgcaaagga ttggttttca gacggcgttt gctgtcttca tccaatttat cttcataacg
     7081 ggtcagatat tcaaccaatg ccgcacggtg tgccgcgcgt tcctcacggt tgcccaagct
     7141 gttgatttcc aaagtcaggt attcgcgaat acccaatttt tcccacaagt cggcagacat
     7201 cgcgatgatt tccgcatcaa tatccggacc ttcaaaaccc aaagcttcga taccgacctg
     7261 atggaactga cgataacggc ctttttgcgg acgctcgcgg cggaacatcg gccccatata
     7321 ccacagcttt tgcgggctgt tgtagagaag gttgtgttcg accaccgcac gcaggcagga
     7381 agccgtacct tcaggacgca agctcaaact caaagaatcg tttgaatcgg agaaggtgta
     7441 catttccttg ccgaccacat cggtttcctc gccgatggag cggacaaaca aaccggtttg
     7501 ctcgacaatc ggcgtacgga tttgctgata accgtaagtg cgtgtccagc ggccgaccgt
     7561 atcttcaaac gcctgccaaa acgcagccgt cagtttgaaa tctttttgct cgacaggcag
     7621 aaggtcgttc atgcctttga cggattggat tttttgtgcc atttcaagta agaatgctta
     7681 aatcaaattg cgggcgatta tagcggattt ttaagggttt gtgaggttgg gggtggtttg
     7741 cggacggcat ttgacttact ctgcacgtgc ttgcctgatt tgttcgactg caaactccgt
     7801 ctgccgcttg ggatgatgtg tgaaaaacaa tttatttgaa attgtctcgg cttttttcgg
     7861 cacgaccgcc aaaatcttac ctgccaaatt tccctcacgg gtttgccaag catccaaaaa
     7921 ctgcgccctg ctcatcgaaa catgccccag cgacgggtcg gcaagcaaaa ccgtattgcc
     7981 gtctatcccg cgcaataccg agaaatgatc atccttgcgg tatttcagat acacgatgac
     8041 ggggattttc aactgcgcga gctgctcgaa agacagggca tagcctttcg cttcaaaacc
     8101 caaatcaggc ataatgcgcc gcatatcctc aaacgacgcg cgcatctgct ccttatccag
     8161 ctttttcaac acttcttctt ccgtcagcgt ttgcccgtaa aaattgttca aaagcgtcgc
     8221 caccgaagcc gccccgcagg aaaaatccaa atcctgcttt acaatattga aatcgcgcct
     8281 ttctttccaa ctctgcactt tgatttttcc gtaaacaaca ggattatcgt taaacatcgg
     8341 tgcagcattc aaacgataag ataaagaaac gacaacacac gccaacagaa aaacatattt
     8401 gaacttcatc atattttcca cataaagagc agcctgaaaa tctttcaggc tgcccttgtc
     8461 aaattattcc tagctttcgg cttttttggc aaaccaaaca atccgattac ccgcataata
     8521 ctttccattt attgaaatcc gacaagccgc gcccaaaaaa tgccatgcac tgtcgatttc
     8581 cgcagcaatc tttgtaccgt tttcttcaaa ttccaaatat tcacccaata ataaacttga
     8641 aacagaacgc gtgggcagca agtgcccacc ctacgcttat tcaaataatt tgattaaata
     8701 aagaataaag aaaatgcatc aggaacaaaa ttataatctg ccacctgact cacaccgctt
     8761 ttaaagtaag gggcatcaaa atcaaaaccg caaaaaaaat aatttttgca ttgattttta
     8821 atagatttaa aattcgaata tagtgtttct ccagatttaa aaagtttatc tttatctata
     8881 tatttgatgc tttccctatc caaaataaat atttcaaaca ttaaaaaatc attacatgac
     8941 caagccaaat cataaatttg attcaaatgg gtatcataaa gataaaaata ataaggtttg
     9001 ggaacaggta aaatatttaa tggaaaagga ggactaattt tcttaatatc tgatgactga
     9061 tatccatatc tttctacatt cttaaaaatt tcatctttct caagataaca agacattctg
     9121 acaatcacaa cctgttcatc ggatatatta tctatttgca tggcgcgtat aacacgccat
     9181 gcctgattaa aattagtctc ccttacctta gaatctatta tcttttccaa agatgaaaat
     9241 gccccttatc ttgaactctc cagttagaac ctggaagttt cgtacctctt aaatgtgtat
     9301 taatatttct tatagtttca ttaaaatgcc acgcgctgcc gatttcaacg gtaattttcg
     9361 taccgctttc ttcaaattcc aggtattccc ccattagcta acgcaaagaa gcagacgcca
     9421 tttcggcttc gttatgataa acccgccttc cgttgatata gacttccgcc cctgtccgct
     9481 ccaaattcca aaaattccgt actgaaattt ccatatcccg atattgtgca gaccatgttt
     9541 tttcgaaggt tttcataaaa tttcctatac ctgtccaatc ggcacatatc aattgcatt
//
LOCUS       DAWFHY010000077         9458 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.077, whole genome shotgun sequence.
ACCESSION   DAWFHY010000077 DAWFHY010000000
VERSION     DAWFHY010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9458
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.077"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(98..457)
                     /gene="rnpB"
                     /locus_tag="WGQ68_001658"
     ncRNA           complement(98..457)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="WGQ68_001658"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(536..1639)
                     /gene="prfB"
                     /locus_tag="WGQ68_001659"
     CDS             complement(536..1639)
                     /gene="prfB"
                     /locus_tag="WGQ68_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEZ0715426.1"
                     /translation="MEAEVINQLNNTLNDLEKRSEDIRVYMDYQGKKDRLEEVIGLSE
                     DPELWNDPKRAQEIGKERKILEGIVLTLDNIASGIEDNRMLIEMTVEENDEEGFAAVQ
                     EDVAGLEKQMADLEFKRMFNQPADPNNCFIDITAGAGGTEAEDWAGMLFRMYSRYAER
                     KGFKIEILEEDDGEIAGINRATIRVEGEYAYGLLRTETGVHRLVRYSPFDSNNKRHTS
                     FASVFVYPEIDDSIEIEINPADLRIDTYRASGAGGQHINKTDSAVRITHEPTGIVVQC
                     QNDRSQHANKAAAMEMLKSKLYELEMRKRNEEKQALEEGKSDVGWGSQIRSYVLDSSR
                     IKDLRTGYEVGNTKAVLDGDLDGFIEASLKQGV"
     gene            complement(1742..2635)
                     /locus_tag="WGQ68_001660"
     CDS             complement(1742..2635)
                     /locus_tag="WGQ68_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEZ0715427.1"
                     /translation="MVTFSKIRPLLAIAAAALLAACGTAGNNAARKPVQTAKPAAVVG
                     LALGGGASKGFAHVGIIKVLKENGIPVKVVTGTSAGSIVGSLFASGMSPDRLELEAEI
                     LGKTDLVDLTLSTSGFIKGEKLQNYINRKVGGRQIQQFPIKFAAVATDFETGKAVAFN
                     QGNAGQAVRASAAIPNVFQPVIIGRHTYVDGGLSQPVPVSAARRQGANFVIAVDISAR
                     PGKNISQGFFSYLDQTLNVMSVSALQNELGQADVVIKPQVLDLGAVGGFDQKKRAIQL
                     GEEAARAALPEIKRKLAAYRY"
     gene            2977..3426
                     /locus_tag="WGQ68_001661"
     CDS             2977..3426
                     /locus_tag="WGQ68_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEZ0715428.1"
                     /translation="MKIGTTWQTASAMLVLRLFAAYEFLESGLQKWNGENWFSEINDQ
                     FPFPFNLLPDALNWNLAMYAELLLPVLLLLGLATRLSALGLMVVTAVAWAAVHAGSGY
                     NVCDNGYKMALIYIVVLIPLLFQGAGGWSLDTLLKKRFCPQCRLKQD"
     gene            3500..3808
                     /locus_tag="WGQ68_001662"
     CDS             3500..3808
                     /locus_tag="WGQ68_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003688122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715429.1"
                     /translation="MNKNIAAALAGALSLSLAAGAVAANKPASNATGVHKSAHGSCGA
                     SKSAEGSCGAAGSKAGEGKCGEGKCGATVKKTHKHTKASKAKAKSAEGKCGEGKCGSK
                     "
     gene            3924..4766
                     /locus_tag="WGQ68_001663"
     CDS             3924..4766
                     /locus_tag="WGQ68_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HEZ0715430.1"
                     /translation="MIQHAGLGYRRDLAEDFLSLSENSPICFIEAAPENWLKMGGWAR
                     KQFDRVAERLPLALHGLSMSLGGQAPLDTDLIDGIKEMMRRYDCTFFSDHLSYCHDGG
                     HLYDLLPLPFTEEMVHHTARRIREVQDRLGCRIAVENTSYYLHSPLAEMNEVEFLNAV
                     AREADCGIHLDVNNIYVNAVNHGLLSPEAFLENVDAERVCYIHIAGHDVETPELLIDT
                     HGAAVLPTVWDLLELAYAKLPTIPPTLLERDFNFPPFAELEAEVAKIADYQTRAGKEY
                     RRAA"
     gene            4756..5502
                     /locus_tag="WGQ68_001664"
     CDS             4756..5502
                     /locus_tag="WGQ68_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2063 domain-containing protein"
                     /protein_id="HEZ0715431.1"
                     /translation="MQPETSAQYQHRFAQAIRGGEAADGLPQDRLNVYIRLIRNNIHS
                     FIDRCYTETRQYFDSEEWSRLKEGFVRDAHAQTPYFQEIPGEFLQYCQSLPLSDGILA
                     LMDFEYTQLLAEVAQIPDIPDIHYSNDSKYTPSPAAFIRQYRYDVTDDLQEAETALLI
                     WRNAEDDVMYQTLDGFDMMLLEIMGSSALSFDTLAQTLVEFMPEDDNWKNILLGKWSG
                     WTEQRIIIPSLSAISENMEGNSPGQNHLSA"
     gene            5524..6111
                     /locus_tag="WGQ68_001665"
     CDS             5524..6111
                     /locus_tag="WGQ68_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEZ0715432.1"
                     /translation="MPLPDLTDAELIESRKLLLHFARLQLPDHPDLAEDLVQETLLSA
                     YSAGDSFQGRALVNSWLFAILKNKIIDALRQIGRQRKVFTTLDDELLDEAFESHFSQN
                     GHWTPEGQPQHWNTPEKSLNNNEFQKILQSCLYNLPENTARVFTLKEILGFSSDEIQQ
                     MCGISTSNYHTIMHRARESLRQCLQIKWFNQENPK"
     gene            6117..6296
                     /locus_tag="WGQ68_001666"
     CDS             6117..6296
                     /locus_tag="WGQ68_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003692066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEZ0715433.1"
                     /translation="MKKCRDIALLLSKHQDRETTPGEKISIYTHLLFCPYCREYKRQL
                     QTIKRSLAKTTRTSK"
     gene            6528..6968
                     /locus_tag="WGQ68_001667"
     CDS             6528..6968
                     /locus_tag="WGQ68_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715434.1"
                     /translation="MIKETLMRPIFLSFVLLPILITACSTPDKSARWENIGTISNGNI
                     HTYINKDSVRKNGNLMIFQDKKVVTNLKQERFANTPAYKTAIAEWEIHCNNKTYRLSS
                     LQLFDTKNTEISTQNYTASSLRPMSILSGTLTEKQYETVCGKKL"
     gene            complement(7497..7853)
                     /locus_tag="WGQ68_001668"
                     /pseudo
     CDS             complement(7497..7853)
                     /locus_tag="WGQ68_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004467771.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            7913..8521
                     /locus_tag="WGQ68_001669"
     CDS             7913..8521
                     /locus_tag="WGQ68_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0715435.1"
                     /translation="MLRFFVLEVTARSAADILGIHPNSAALFYRKIRMVINHHLALAA
                     DEVFEGPVEPDESDFGGRRKGRRGRGAAGKVVVFGILKRNGRVYTVVVDNAKSETLLP
                     VIKKKIMPDSIVYTDSLSSCDKLDVSGFIHYRINHSKEFADRQNHINGIENFWNQAKR
                     VLRKYNGIDCKSFPLFLKECEFRFNFGTPSQQLKILRDWCGI"
     gene            complement(8592..9161)
                     /locus_tag="WGQ68_001670"
     CDS             complement(8592..9161)
                     /locus_tag="WGQ68_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEZ0715436.1"
                     /translation="MLFPRIIAASAQRKLSVYLKKGGLVAYPTESCYGLGCLPTLAKA
                     LGKLAHLKKRPQHKGMIVIGNQFEQLQPLLQMPSENLQDMLRKEWPAPKTFLLSAKSC
                     VLPELRGKQRSKLAVRVPAHVGARRLCQALQTPLVSTSCNRAGKRACRTEREVRRQFG
                     RDVWIVGGRIGRQKSPSQIIDGETGKRLR"
     gene            complement(9313..>9458)
                     /locus_tag="WGQ68_001671"
     CDS             complement(9313..>9458)
                     /locus_tag="WGQ68_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0715437.1"
                     /translation="FGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANRLSAP
                     AAA"
BASE COUNT         2491 a         2473 c         2103 g         2391 t
ORIGIN      
        1 gtaccggttt ttgttaatcc actataaaaa cacaacctaa ataaaaatgc cgtctgaacc
       61 atatttcagg tttcagacgg catttgcgta tcggatgcac accggacggg cggtaagccg
      121 ggttctgtct cggacagtca ttcctctagg cataccgtta ccggtatgct caagcaacct
      181 acccgaacgc tcggcgggca gcgtcattgc gttctgtttg gtcttgctcc gaatggggtt
      241 tggcctgccg catattgtta ccaaatgcgc ggtgcgccct taccgcacct tttcaccctt
      301 gcctgtgctg ccaaagcagc catcggcggt tttgctttct gttccacttt ccgtcgcgtt
      361 accgcgcccg gccgttaacc ggcattctac cctgcggagc ccggactttc ctccccgtat
      421 gccttacgcg atacgcggcg actgtctgct cgtcccgtgt gcggcgcgga ttataacacg
      481 aaacgcaaaa atgccgtctg aaacggtaca ggtttcagac ggcatacggt tcaaactaca
      541 cgccctgttt caggctggct tcgataaagc cgtccaaatc gccatccaat acggctttgg
      601 tgttgccgac ttcgtagcct gtacgcaagt ctttgatacg tgaggaatcc aaaacatacg
      661 aacggatttg gctaccccaa cctacatcgg atttaccttc ttccaacgcc tgtttctctt
      721 cattgcgttt gcgcatttcc aattcataca gtttggactt caacatttcc atcgcggcgg
      781 ctttgttggc gtgttgcgaa cggtcgtttt gacattgcac cacaatcccc gtcggctcgt
      841 gggtaatgcg cacggcggag tcggttttat tgatgtgctg accgcccgcg cccgatgcgc
      901 gataggtgtc gatacgcaaa tcggcggggt tgatttcgat ttcaatggaa tcatcgattt
      961 cagggtaaac gaacacggag gcaaacgaag tatggcgttt gttgttcgag tcaaacggtg
     1021 aatagcgcac caagcggtga acgccggttt cggtacgcag caaaccatag gcgtattcgc
     1081 cttccactcg gatagtcgcg cggttgatgc ccgcgatttc gccgtcgtct tcttcgagga
     1141 tttcgatttt gaagcctttg cgctcggcgt agcggctgta catacggaac agcatacccg
     1201 cccagtcttc cgcttccgta ccgcccgcgc ctgcggtgat gtcgataaag cagttgttcg
     1261 ggtcggcggg ctggttgaac atccgtttga actccaaatc cgccatctgt ttttccagcc
     1321 ccgccacatc ttcctgcacg gcggcaaaac cttcttcgtc gttttcttcg acggtcattt
     1381 caatcagcat gcggttgtct tcgatgcccg aagcgatgtt gtcgagcgtc aacacgatgc
     1441 cttcgaggat tttgcgctct ttgccgattt cttgggcgcg tttcgggtcg ttccaaagtt
     1501 cggggtcttc ggaaagaccg ataacttctt ccaatcggtc tttcttaccc tgataatcca
     1561 tataaactcg gatgtcttcg ctgcgctttt ccaaatcatt cagggtattg ttgagctggt
     1621 tgattacttc ggcttccatg attcttttgt tctttcaaaa ttttaggggc gtattgtacg
     1681 ggattcgggt atttttttct atggataaag ccttctagaa acacgttcgg acggcatagc
     1741 gtcaataacg gtatgccgcc agtttgcgtt tgatttcagg caatgcggca cgtgctgcct
     1801 cctcacccaa ctggatggca cgttttttct gatcgaatcc gccgactgca cccaaatcca
     1861 aaacctgcgg tttgataacc acatccgcct gccccaactc attttgcaac gcggaaacgc
     1921 tcattacgtt cagcgtctga tcgagataag agaagaagcc ttggctgatg tttttgcccg
     1981 gacgggcgga aatatcgacg gcaatcacga aattcgcccc ctgccgccgg gcggcactga
     2041 cgggcacggg ctgcgacaga ccgccgtcaa catatgtatg cctgccgatg ataacgggtt
     2101 ggaacacatt gggaatggcg gcggaagcgc gcacagcctg cccggcattc ccttgattga
     2161 aagcgacggc cttgccggtt tcaaaatcag tagcaacagc ggcaaatttg atgggaaact
     2221 gctgaatctg cctgccgccg acttttcggt tgatgtaatt ttgcagcttt tcgcctttga
     2281 taaaaccact ggtggacaag gttaaatcga ctaaatcggt tttgcctaaa atttcggctt
     2341 ccaattcgag gcggtcgggc gacatacccg atgcaaaaag gctgccgaca atcgaacctg
     2401 ccgatgtgcc ggtaaccacc ttcacaggaa taccgttttc tttcaaaacc ttaataatac
     2461 ctacatgggc aaatccttta gatgcgccgc caccgagtgc caaaccgacc actgcggcgg
     2521 gtttggcggt ttgcaccggc ttgcgggcgg cattatttcc cgccgtgccg caggcggcaa
     2581 gcaacgcggc ggcggcgatt gccaaaagcg gtctgatttt tgaaaacgtt accatatttt
     2641 ccattccttt atatatcgca ccccgtcaaa aagagggatt gcttttctta acaaccccct
     2701 ttgacagcca agcaaatggg ggctttgtta agtcatcatc aaaattaata tttctttttt
     2761 tcctttacgg aaattatatt tgaaggcata ctatccaagg tgggaattat ctcacaacac
     2821 cgccgttatc caaatatccc gcctttttcc cttcctttcc atcaaaatac tttcttttta
     2881 tattcattaa cttgttaaat cattggctgc cgggtgtcag tttttccgac aaaatccgtc
     2941 taatggggta tcaacagaac caaaacagga acacttatga aaatcggaac aacttggcag
     3001 acggcatccg ctatgctggt tttgcgtctg tttgccgcat atgaattttt ggaatcgggt
     3061 ttgcaaaaat ggaacgggga gaattggttt tctgaaatca acgatcaatt cccattcccg
     3121 ttcaacttgc tgccggacgc gttaaactgg aatctcgcta tgtatgcgga gcttttactg
     3181 cccgtactgt tgcttttggg tttggcaacg cgtctgtcgg cattagggct gatggtcgtt
     3241 accgccgtcg cttgggctgc ggttcacgcc ggttcgggtt acaatgtctg cgacaacggt
     3301 tataaaatgg ctttaattta tatcgtggta ttaatcccgc tgcttttcca gggtgcgggc
     3361 ggatggtcgc tggatacgct gctgaaaaaa cggttttgcc cccaatgccg tctgaagcaa
     3421 gattgattca gtcgtggaat ctgactttaa acattccaac cttatctcgt taacttgata
     3481 ttctgaaaag gaaatagaaa tgaacaaaaa cattgctgcc gcactcgccg gtgctttatc
     3541 cctgtctttg gccgccggtg cagttgctgc caacaaaccg gcaagcaacg caacaggcgt
     3601 tcataaatcc gcccatggct cttgcggcgc gtccaaatct gccgaaggtt cgtgcggcgc
     3661 ggctggttct aaagcaggcg aaggcaaatg cggcgagggc aaatgcggtg cgaccgtaaa
     3721 aaaaacccac aaacacacca aagcatctaa agccaaggcc aaatctgccg aaggcaaatg
     3781 cggcgaaggc aaatgcggtt ctaaataatc ccaccccttc aaaccaagcc gcgtttttca
     3841 gtaaaatgcg gcttttttaa cggcaaacaa agatttttta acaagcacat cattcttttg
     3901 tgccatccga accgggtaaa aatatgattc aacacgcagg cttgggctac cgccgcgact
     3961 tggcggaaga ctttctctcg ctttccgaaa acagcccgat atgctttatc gaagccgcac
     4021 cggaaaactg gctgaaaatg ggcggctggg cgcgcaaaca gtttgaccgt gtggcggaac
     4081 ggctgccgct ggcgttgcac ggattgtcta tgtcgctggg cgggcaagca ccgctggata
     4141 ctgatttgat agacggcatc aaagaaatga tgcgccgtta cgattgcacg tttttctccg
     4201 accatttgag ctactgccac gacggcggtc atctttacga tttgttgccg ctgcccttta
     4261 ccgaggaaat ggtgcatcat acggcgcggc gtatccgcga agtgcaagac cgtttgggct
     4321 gccgcatcgc cgtggaaaac acgtcctact atctgcattc cccgcttgcc gagatgaacg
     4381 aggtcgagtt cctcaacgcg gttgcccgtg aagccgactg tggcattcat ctcgatgtga
     4441 acaatatcta cgtcaacgcc gtcaatcacg gcctgttgtc gcctgaagcc tttttagaaa
     4501 atgtggacgc agagcgcgtg tgctatatcc atattgccgg acatgacgtg gaaacgcccg
     4561 aattgttgat tgatacgcat ggcgcggcgg ttttgccgac cgtttgggat ttgctcgaac
     4621 tcgcctatgc caagctgccg acgattccgc ccaccctgtt ggaacgcgat ttcaattttc
     4681 cgccttttgc cgaactcgaa gccgaagtcg ccaaaattgc cgactatcaa acgcgtgccg
     4741 gaaaggaata ccgccgtgca gcctgaaacc tccgcccaat accagcaccg tttcgcccaa
     4801 gccatacgcg ggggcgaagc cgcagacggt ctgccgcaag accgactgaa cgtctatatc
     4861 cgcctgatac gcaacaatat ccacagcttt atcgaccgct gctataccga aacgcggcaa
     4921 tactttgaca gcgaagaatg gagccgtctg aaagaaggtt tcgtccgcga cgcgcacgcc
     4981 caaacgccct attttcaaga aatccccggc gagttcctac aatattgcca aagcctgccg
     5041 ctttcagacg gcattttggc actgatggat tttgaatata cccaattgct ggcagaagtt
     5101 gctcaaattc cggatattcc cgacattcat tattcaaatg acagcaaata cacaccctcc
     5161 cctgcggcct ttatccggca atatcgatat gatgttaccg atgatttgca ggaagcggaa
     5221 acagccttgt taatatggcg aaacgccgaa gatgatgtga tgtaccaaac attggacggc
     5281 ttcgatatga tgctgctgga gataatgggt tcctccgcgc tttcgtttga caccctcgcc
     5341 caaacccttg tcgaatttat gcctgaggac gataattgga aaaatatttt gcttgggaaa
     5401 tggtcaggct ggactgaaca aaggattatc atcccctcct tgtccgctat atccgaaaat
     5461 atggaaggca attccccggg ccaaaaccat ctatccgcat aaaattacct tgttcccgat
     5521 actatgccgc tacccgacct gaccgatgcc gaattaatag agtcgcgtaa actgcttctg
     5581 cattttgcgc ggcttcagtt gcccgaccac cctgatttgg ctgaagattt agtgcaggaa
     5641 acattgctgt ccgcatacag cgcaggcgac agttttcaag gcagggcact tgtcaacagc
     5701 tggctttttg ccatattgaa aaacaaaatt attgacgcat tacgtcaaat cggaaggcag
     5761 aggaaagtct ttaccacact ggatgacgag ctgctggatg aagcatttga aagccatttt
     5821 tcccaaaacg ggcattggac gccggaaggg cagccgcaac attggaacac tccggaaaaa
     5881 tcattaaaca acaacgaatt ccaaaaaatt ctgcaaagct gcctatacaa cctgcctgaa
     5941 aacaccgcac gggtatttac cctgaaggaa atactcggtt tttcatccga cgaaatacaa
     6001 caaatgtgcg gtatcagcac gtccaactac cacaccatta tgcaccgcgc ccgagaatca
     6061 ttgcgccaat gcctgcaaat caaatggttc aaccaagaaa acccgaagta aacgttatga
     6121 aaaaatgccg cgatatcgcc ctgcttcttt ccaaacatca ggaccgggaa accaccccgg
     6181 gcgagaagat ttccatatac acacacctgc tgttctgtcc gtattgccgt gaatataaaa
     6241 gacaacttca aaccatcaaa agatcactgg caaaaacaac cagaacttca aaataaatgc
     6301 cgtctgaaaa ggcttcagac ggcataagct gacggaaaca aatcaaaccg atttactgtt
     6361 atcggcagtt catccataat acacgcttca aaagcagcat attcctccat acggaatata
     6421 taaatacgca aaacacgaag gctgcatcaa tttgctttat ttgccttatt tcacagacgg
     6481 cgctacccct cccgcccaac ccgttctttc tgaatgagca gatttcaatg attaaggaaa
     6541 ccctaatgcg cccaatcttc ctatctttcg ttttactccc tattttgata accgcctgca
     6601 gcacaccgga caagtctgcc cgatgggaaa atatcggcac aatctcaaac ggcaatattc
     6661 atacatatat caataaagac agcgtgagaa aaaacggaaa tctgatgatt ttccaagata
     6721 aaaaagttgt taccaatcta aaacaagaac gttttgccaa cacccccgca tacaagactg
     6781 ccattgccga gtgggaaatc cactgcaaca acaaaacata ccgcttaagt tcgctacagt
     6841 tgtttgatac aaaaaacacg gaaatttcca cacaaaacta cacagcctct tccctccgcc
     6901 cgatgagcat cctgtccggg acattaaccg aaaaacaata tgaaaccgta tgcggaaaaa
     6961 aactctgatt gcaacttata cacaaactta cccacaaacc ttatcataaa aatgccgtct
     7021 gaaatactga aatatcagca tttcagacgg cattttgcca ttccctgaaa attatccaca
     7081 aagttatcca cattattttt taaaaccggc ttccatccga aatatagtgg attaacaaaa
     7141 atcaggacaa ggcgacgaag ccgcagacag tacagatagt acggcaaggc gaggcaacgc
     7201 cgtactggtt taaagttaat ccactatata aactcgctat acaatttcac tatccaaacg
     7261 taaattgttc cattgataca caaaacttct tacccccata attttgataa agcatttctt
     7321 acattcccgg ctccgtcccg taaccaacac agcctctaaa aaataatcgc ttgttttgac
     7381 accgatacac tcatatagtg gattaacaaa aatcaggaca aggcgacgaa gccgcagaca
     7441 gtacagatag tacggcaagg cgaggcaacg ctgtactggt ttaaatttaa tccactatac
     7501 aaatacagaa actcaagaaa ataaccttgt gtattgacca tctcaagcaa ttcagaaaaa
     7561 tcaagaaatt ttctgaccgt aaacaaacgt ttccctaaaa aaacgatgtc ttcaaaaata
     7621 tcgaccaaat aatcatcttc gccgttttca tttttatggg ttaaaacaac acgggaaaaa
     7681 tctgttttca gatgcttgcc cgcttgattg ttcggattat tgtccggaac gacaaaaccg
     7741 tcctcaaaat caaagtagac gttgcgtcct tctaccttta tctctgtgca ataacaatca
     7801 tgcagagaaa taccatccga aaaatttagg ggctgtacta gattagcaga tatgttaccc
     7861 tcgaaatatg aagataacgc actgcaaatg aaagaaaaaa gtacagaaag aactgctccg
     7921 tttttttgta ctcgaagtta ccgcccgttc tgccgccgat attttgggta tccatccaaa
     7981 ttcggcagca ctgttctacc gtaaaatccg catggttatc aaccatcatt tggccttggc
     8041 tgccgatgag gtttttgagg gccctgtcga gccggacgaa agcgatttcg gcggacggcg
     8101 taaaggtaga cgtggtcgcg gtgcagcagg aaaagtggtt gtcttcggca ttctgaaacg
     8161 caacggacgg gtctataccg ttgtggtgga taatgccaag tctgaaacgt tactccctgt
     8221 catcaaaaag aaaatcatgc cggacagtat tgtttatacc gatagtctga gcagctgcga
     8281 caagttggac gtgagcggtt ttatccatta ccgcatcaac cattccaagg aatttgcaga
     8341 ccgtcagaac cacattaacg gcattgagaa tttttggaat caggcaaaac gcgtcttgcg
     8401 aaaatacaac ggaatcgatt gcaaatcttt cccactgttc ttgaaagaat gcgaatttcg
     8461 atttaacttc ggcacaccgt ctcaacagct taaaatcctg cgggattggt gtgggattta
     8521 gggctaatct agtacagccc caaaattttt tctttgtgta tgcaaaaaag ttttcattca
     8581 agtacccata tctaacgcaa acgtttacct gtttccccgt caataatctg actcggcgat
     8641 ttctgcctgc cgattctccc accaacaatc cacacatcgc gtccgaattg ccttctgact
     8701 tccctctccg tccgacacgc gcgtttgcct gcgcggttgc acgaagtcga gaccaaaggc
     8761 gtttgcaaag cctgacacaa gcggcgcgca cctacatggg cgggaaccct gaccgccaac
     8821 ttgctgcgct gtttcccgcg caactcaggt aagacacagg atttggcgga taataggaac
     8881 gttttagggg caggccattc ttttctaagc atatcctgaa gattttcaga cggcatttga
     8941 agtaaaggct gcaattgttc aaattgattc ccgatgacaa tcataccctt gtgttgcggt
     9001 ctttttttca aatgcgccaa cttaccgagt gctttggcta atgtcggaag acaccccaag
     9061 ccataacaag attcggtcgg ataagcgacc aaaccgcctt ttttcaaata aacgcttaac
     9121 ttacgttgcg ctgatgctgc gataattctc ggaaataaca taatgagacc tttgcaaaat
     9181 tcctttccct cccgacagcc gaaacccaaa cacaggtttt cgtctatttt cgccccaaat
     9241 accgcctaat tttacccaaa taccccctta atcctccccg gatacccgat aatcaggcat
     9301 ccgggctgcc ttttaggcgg cagcgggcgc acttaacctg ttggccgctt tcaacaggtt
     9361 caaacacatc gccttcaggt ggctttgcgc actcacttta atcagcccga aataggctgc
     9421 ccgagcgtag cggaatttac ggtgcagcgt accgaagc
//
LOCUS       DAWFHY010000078         9405 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.078, whole genome shotgun sequence.
ACCESSION   DAWFHY010000078 DAWFHY010000000
VERSION     DAWFHY010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9405
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.078"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(104..622)
                     /locus_tag="WGQ68_001672"
     CDS             complement(104..622)
                     /locus_tag="WGQ68_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HEZ0715438.1"
                     /translation="MAKTRIKQPAIEAAQDKTEVTAFIRQIGDLQREVKRLETEAGDK
                     KAAIEEEYAAKAAPMCAEIMSLTERVAAYCEAHKDELTENGKTKTVDFTTGLIKWRIR
                     PPSVKVTGVAAVLAWLSEKSAFAEFVRTKKEIDKDTILNQKECFSDGQVPGIKIVSGV
                     EDFVIEPSEQIL"
     gene            complement(637..864)
                     /locus_tag="WGQ68_001673"
     CDS             complement(637..864)
                     /locus_tag="WGQ68_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715439.1"
                     /translation="MKVYVFKISNENGKLKIELPEIPMGKQIDEVDLIAGLTTEFIAS
                     MLRDAQKDRRKFVIDASNQLAAIQTYQKIFN"
     gene            complement(928..1095)
                     /locus_tag="WGQ68_001674"
     CDS             complement(928..1095)
                     /locus_tag="WGQ68_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715440.1"
                     /translation="MNEKDLIEWLEDRGELMVMKKDGEGFVIAARAPDGMWKTAEAET
                     LAQAITLWEEV"
     gene            complement(1088..1261)
                     /locus_tag="WGQ68_001675"
     CDS             complement(1088..1261)
                     /locus_tag="WGQ68_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715441.1"
                     /translation="MSFGRRNTDWQAWGQHRRRATARMAQKSREREIEEYQAQFEDKD
                     GKGRLKTEGNKDE"
     gene            complement(1370..1636)
                     /locus_tag="WGQ68_001676"
     CDS             complement(1370..1636)
                     /locus_tag="WGQ68_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715442.1"
                     /translation="MQVLKKVDWKMFVARSFWQCVPVGLTAGVWCFVAGMALHSCAKE
                     PEPVAKEPTKVEAMERQADLEVKVWEGQYESMSVEEKMKGIVYE"
     gene            complement(1726..2640)
                     /locus_tag="WGQ68_001677"
     CDS             complement(1726..2640)
                     /locus_tag="WGQ68_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEZ0715443.1"
                     /translation="MKQINHTLQQKLAEFKTKSGMNQTQLARGIGTSPASISMYLNGT
                     YAEKGGNYETIEPKIEAFLEMQDSKARREELVLGFVSTKTTRRIVEAMRNAHEGGEIS
                     VIYGQAGLGKTQAVKNYCEKNPAAILIEANPSFTALVLMRKLATAAKVSAMGSLNDLF
                     ESVSDRLRDSGHLIVVDEAENLPLRALEIIRRLHDETGCGLVLSGMPRLVANLRGKYG
                     ELVQLHSRVAGALNLGDTMPDAELEQIARATLPEADDETIAELVKHSNGNTRRMSKLM
                     RGSVRVANKNGIRMQAGIVKKYSTLIIR"
     gene            complement(2676..4667)
                     /locus_tag="WGQ68_001678"
     CDS             complement(2676..4667)
                     /locus_tag="WGQ68_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0715444.1"
                     /translation="MGITIGELLKLNIQGLPTTARGLRERAKTQNWPYIEEVGKARGG
                     RLKKYLIASLPAEIRAAIMKRQSDELTEKMPKTLPQVRPGTAMSAQALAEAAKLLNEK
                     QRSVADARCAVVAAVLGIKYQYGCSAKAAVAQFLGLLAEGKLDAVTLGNLEKANDRSR
                     SAKVGERTLDGWISAYLKAENATERLVALAPKTTKAVKPIESYGWLPMFMQFHNIPSA
                     PKLAHSYRRFVQWAEAENMPVNDVPNLSMVRRVWDKLPMIMQERGRKTGAAYKSLLPY
                     VKRDWGALKPNDVWIGDGHSFKAKVAHPVHGRPFKPEVTVIIDGCTRFVVGFSVSLAE
                     SCVAVSDALRIGVKHFGLPIIYYSDNGGGQIGKTIDHEITGITSRLGIRHETGIAGNP
                     QGRGIIERWWKDNLIEMARQYETFTGAGMDSSTKNLMYRKMESAFNALEKGKDLTEEQ
                     QKYLKKLPSWSRFIADVAKCIDEYNNRPHGELPRHPDGGHYSPKAYREMRLEQDGIAP
                     DMLSAEELATMFMPQEVRKVQRGWLDLFNNSYFSTELAEYHKDEVRVSYDLSDASAVN
                     VFDMDGKFITKAQADGNTREAFPTARIDQLAEKRRKGKIKRAENAIKLANAEVNPALE
                     QAAAWDELGHLGGNDIEAEYAVLPKTGTDDFVLFEADRS"
     gene            complement(4678..4866)
                     /locus_tag="WGQ68_001679"
     CDS             complement(4678..4866)
                     /locus_tag="WGQ68_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEZ0715445.1"
                     /translation="MGIKTVNEVREWLKDNNLTVAQWARENGHNSVDVTRVLTGKSKC
                     RYGQGRAIAIKLGLRIDE"
     gene            4986..5348
                     /locus_tag="WGQ68_001680"
     CDS             4986..5348
                     /locus_tag="WGQ68_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003741237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEZ0715446.1"
                     /translation="MDNSFELSSKLLGERLKKIRTDNGYSREQLSELFGISRASIQNY
                     ENGERSPNADYLVQFYKHFGINLHWLLTGNKAAKFQDFIDSVSSPREEVLLHLARQMD
                     SRTLNHLIDFLMDVYAKK"
     gene            5624..6064
                     /locus_tag="WGQ68_001681"
     CDS             5624..6064
                     /locus_tag="WGQ68_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0715447.1"
                     /translation="MGNPVDLSEFVFVPRYNVAAAAGYGAPVFGEEPLFCLAFRKYWI
                     DNYVTKQPEKLSVITVKGDSMEGVLNHGDSILINHAKTDPRDGLYVLRIGNDLFVKRV
                     QRIPGKLLVTSENPRYAPFEIDFSNAQDDIAIIGRVEWYGRSID"
     gene            6216..6863
                     /locus_tag="WGQ68_001682"
     CDS             6216..6863
                     /locus_tag="WGQ68_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HEZ0715448.1"
                     /translation="MSAFFTLFVPCAKTNSPPQENVALFRHNGEILLSGINIKLKSGD
                     SLLIRGPSGCGKTSLLRALAGLWPFGSSGKVSRPPHQDILFLPQRPYTAQGSLRDAVC
                     YPDIDKQHPELIEAMNTCRLGYLIDKLDKTDDWQHKLSLGELQRVAFVRALLSKPKIV
                     LLDEATAALDEPAEAALYRALKQKLPDSIIISIGHRSTLNAFHNMRLDVGNVACG"
     gene            6968..7636
                     /gene="hda"
                     /locus_tag="WGQ68_001683"
     CDS             6968..7636
                     /gene="hda"
                     /locus_tag="WGQ68_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HEZ0715449.1"
                     /translation="MNQLIFDFAAHDYPSFDKFLGTENAELVYVLRHKHGQFIYVWGE
                     EGAGKSHLLQAWVAQALEAGKNAAYIDAASMPLTDAAFEAEYLAVDQVEKLGNEEQAL
                     LFSIFNRFRNSGKGFLLLGSEYTPQQLVIREDLRTRMAYCLVYEVKPLTDQEKIDALA
                     SMAAARQVTVDSEIFEYLLKHWRRDMDSLMMMLDTLDNYAVTMGKRITLPLLRQLLKQ
                     QETQ"
     gene            7633..8301
                     /locus_tag="WGQ68_001684"
     CDS             7633..8301
                     /locus_tag="WGQ68_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEZ0715450.1"
                     /translation="MKNLAIFDLDNTLINTDSDHAWPQYLIKKGLVDAAETEAQNEKF
                     YRDYQNGCLDIDAFLKFHLAPLARYSKEELAEFHREFMAEYIISHISPMQRMLVQSHQ
                     MAGDETLVISSTNEFIITPICHLFGITNIIGTQLETDPDGRYTGNYIGTPSLKEGKIT
                     RLNQWLAERGETLQSYGKTYFYSDSRNDLPLLRLVSEPVTVNPDAELEKEAKEKGWPV
                     LNFK"
BASE COUNT         2365 a         2623 c         1895 g         2522 t
ORIGIN      
        1 ctttaaatca tgggttaaag cctatttgaa ccggctaggc aagctcctgc tccgtaggct
       61 caatcacgtt tcaacacact gaattgaaat caggctgttg attttataga atttgttcgc
      121 tcggctcaat cacaaaatcc tccacgccgc tcacaatctt aatccccgga acttggccgt
      181 ctgaaaaaca ctctttttga ttcaggatgg tgtctttgtc gatttccttt ttagtgcgga
      241 caaactcggc aaaggcggat ttttccgaga gccacgccag gacggcggcc acgcccgtta
      301 ccttgacaga tggcggacgg atgcgccatt taatcaggcc ggtggtaaag tccacggttt
      361 tggttttacc attttccgtc agctcgtcct tatgcgcttc gcagtaggcg gccacacgtt
      421 cggtcaggct catgatttcg gcacacatcg gcgcggcttt ggcggcatat tcttcttcga
      481 tagccgcttt tttgtctccg gcttcggttt ccaggcgttt gacttcgcgc tgcaagtcgc
      541 cgatttggcg gataaatgca gtaacttccg ttttgtcttg tgccgcttcg atggcgggct
      601 gtttgattcg ggttttagcc attttctctt ccttccttaa ttgaagattt tttgatatgt
      661 ctggattgca gccaattgat ttgatgcgtc gattacgaat ttgcgacgat ctttttgggc
      721 atcacgtaac atacttgcaa taaattccgt ggttagccca gcaatcaaat caacttcgtc
      781 aatttgctta cccattggga tttcggggag ttctatcttt aacttaccgt tttcattact
      841 aattttaaaa acatacactt tcatttcact ttcctttctt gtttaaaatt gcttcaactt
      901 tcgcgcggtt tctcaaaaac ttaaacatca cacttcctcc cataaagtta tcgcctgagc
      961 caaagtttcc gcctcggcag tcttccacat cccgtccggc gcacgcgctg caatcacaaa
     1021 gccttcgccg tccttcttca tgaccatcag ctcgccacgg tcttcgagcc attcgattaa
     1081 atctttttca ttcatctttg tttccttccg ttttcagacg acctttgccg tccttatctt
     1141 caaactgcgc ctgatattcc tcgatttcgc gctcccggct tttttgcgcc attcgcgccg
     1201 tcgcacgcct gcggtgttgt ccccaagcct gccaatccgt attacgtcgt ccgaaactca
     1261 tttcacacat cctttcacaa tcgccttatc gccatatttc gcgcggattt cctttaccgc
     1321 ccgtgccaaa gcctcttttt tcgccgcagg gctcaataaa attggcttat cactcataaa
     1381 caatcccttt cattttttcc tcgacgctca ttgactcgta ttgcccttcc cataccttga
     1441 cctccaaatc cgcctgtctt tccatcgcct caaccttcgt cggctctttc gcaaccggtt
     1501 cgggttcttt cgcgcaggaa tgcaacgcca ttcccgccac aaaacaccac acgcccgccg
     1561 tcaaaccaac cggcacacac tgccaaaaag agcgtgccac aaacatcttc caatcaactt
     1621 tcttcaaaac ttgcattttg agttttcctt tagaaaacaa taacttatta aaatcatagg
     1681 gtaaaaaata tatagccctg tcagagactt accgtttcag acggcctatc ggataatcaa
     1741 tgtcgaatat tttttcacga ttcccgcttg cattcgaata ccgtttttat tcgctacccg
     1801 tactgatccg cgcatcaact tgctcatacg ccgtgtatta ccattgctat gcttaaccag
     1861 ttccgcaatc gtttcatcat cggcttcggg tagggttgcc cgcgcaatct gttccaactc
     1921 tgcgtcgggc atcgtatcac ctagattcag cgcacccgcc acacgactgt gaagctgtac
     1981 caactcgcca tacttaccgc gcaaattcgc caccagtcgg ggcataccgc tcaacaccaa
     2041 accgcagcca gtctcatcgt gcagtcggcg gataatctca agggcgcgta atggcaggtt
     2101 ttccgcttca tcgaccacaa tcagatggcc tgaatcacgc aatctgtccg ataccgattc
     2161 aaacaaatca ttcaagctgc ccatcgccga taccttcgcc gccgtcgcca acttgcgcat
     2221 caagaccaaa gccgtaaagc tcggattagc ctcaatcaag atggctgcag ggtttttctc
     2281 gcagtagttt ttgaccgcct gcgtcttgcc caaacccgcc tgaccgtaga taacggaaat
     2341 ctcgccgcct tcgtgcgcat tgcgcatcgc ttccacgatt cggcgtgtcg tcttagtcga
     2401 tacaaatccc aacaccagct cttcacgtcg cgccttactg tcctgcatct ccaaaaacgc
     2461 ctcgattttc ggctcgatgg tttcataatt gccgcctttt tccgcataag tgccgttcag
     2521 atacatactg atggatgccg gcgaagtacc gataccgcgt gccagttggg tttggttcat
     2581 gcctgatttg gttttaaatt cagccagttt ttgttgcaat gtatgattga tttgtttcat
     2641 ttttttagtc cttttaaaag aggtttaaaa ccgttttaac ttctatccgc ctcaaacaac
     2701 acaaaatcgt ctgtgcccgt tttcggcaat accgcatact ccgcctcgat gtcgtttccg
     2761 cccaaatgtc ccagctcgtc ccaagctgcc gcctgttcca gtgccggatt gacttccgcg
     2821 tttgcgagct tgattgcatt ttccgcccgc ttgattttgc cttttcggcg tttttccgcc
     2881 agttggtcga tacgagccgt cgggaaagcc tcgcgggtat tgccgtcggc ctgcgcctta
     2941 gtgataaact tgccgtccat atcaaacaca ttgaccgccg acgcatcgct caaatcgtag
     3001 ctgacccgta cctcgtcttt gtgatactcc gccagctcag ttgagaaata agagttgttg
     3061 aacaaatcca gccaaccgcg ctgtaccttt cgcacctctt gcggcataaa catcgtcgcc
     3121 agctcttctg ccgacaacat atccggcgcg ataccgtcct gttccagcct catttcccga
     3181 taagccttcg gcgaataatg cccgccgtca ggatgtcggg gcagctcgcc gtgcgggcgg
     3241 ttgttgtatt cgtcgataca cttggccaca tccgcgataa agcgcgacca gctcggcagt
     3301 tttttcaaat atttctgttg ttcctccgtc aaatccttgc ctttttccaa agcattaaaa
     3361 gcactttcca tcttgcggta catcaggttc ttcgtgctgc tgtccatccc cgcgcccgta
     3421 aacgtctcat actgtcgcgc catctcaatc agattgtctt tccaccatcg ctcaatgatg
     3481 ccgcgccctt gcgggttgcc cgcgataccc gtttcatgcc ggatacccag tcgggacgtg
     3541 atacccgtga tttcatggtc tatcgtcttg ccgatttggc cgccgccgtt atccgagtag
     3601 tagataatcg gcaaaccaaa gtgcttgacc ccgatacgca gagcatccga taccgccaca
     3661 caactttcag ccaacgacac cgaaaaaccg accacaaacc gcgtacaacc atcaataatc
     3721 accgtcactt ccggcttaaa cggtctgccg tgtacagggt gcgccacctt cgccttaaag
     3781 ctgtggccgt cgccgatcca aacatcgttt ggctttaaag ccccccaatc acgtttcaca
     3841 taaggcagca gcgatttata agccgccccc gttttcctgc cgcgctcctg cataatcatc
     3901 ggcagcttgt cccaaacgcg ccgcaccata ctcaaattag gcacatcatt gaccggcata
     3961 ttttccgctt cagcccactg cacaaaccgg cggtagctgt gcgccagttt cggcgcggac
     4021 ggaatattgt gaaactgcat aaacatcggc aaccaaccgt agctctcaat cggcttaacc
     4081 gccttcgtcg tcttcggagc taaagcaacc aaccgctccg tcgcgttttc cgctttcaaa
     4141 taagcagata tccagccgtc taaagtacgt tcgccaacct tcgccgaccg gctgcggtca
     4201 ttggcctttt ccaagttccc aagcgtgacc gcgtccaatt taccttctgc cagcaagccc
     4261 aaaaactgag ccaccgcagc cttggcagag caaccgtatt gatatttaat ccccaacacc
     4321 gccgccacca ccgcacatcg cgcatccgcc accgaccgtt gtttctcatt caacagcttg
     4381 gccgcttcag ccagtgcctg agccgacatc gccgtccccg gtctgacttg gggcaaggtt
     4441 ttcggcatct tctccgtcag ctcgtccgac tgccgtttca tgatggctgc tcggatttcg
     4501 gcagggaggg aagcgattaa gtattttttc agacggcctc cacgcgcttt gccaacctct
     4561 tcgatgtacg gccagttctg ggtttttgct ctctccctta atcctcttgc tgttgtagga
     4621 agcccttgaa tattaagttt aagcagctca ccaatcgtta tccccatctt cgtttcctca
     4681 ctcgtcaatt cttaacccca gcttaatcgc gatagcgcgc ccctgaccgt atcgacactt
     4741 tgatttcccc gtcaacacac gagtaacatc tacggagttg tgtccgtttt ctctcgccca
     4801 ttgcgcaact gttaaattat tgtcttttaa ccattctcta acttcattta ctgttttaat
     4861 tcccattttt ttacctttct tggtttgttc aaatcctgta taattactac aaaactagct
     4921 gttttgctac atttgtagca atattattag ctacaaatgt agctgtcaag aaaaaaggaa
     4981 cttttatgga taattctttt gaattaagct ctaagttatt gggtgagcga ctaaaaaaaa
     5041 tcagaacaga taatggatac tcaagagagc agttgagtga gttatttggc atcagtaggg
     5101 caagtattca aaattatgag aatggggaaa gaagtccgaa tgccgattac ttagttcagt
     5161 tttataagca ctttggaatt aatttgcatt ggctgctaac tggaaataag gctgccaaat
     5221 tccaagattt cattgattct gtttcttctc cgagagaaga agtactacta cacttggctc
     5281 gccaaatgga tagtcgcacc ttgaatcact tgattgactt tctaatggat gtttatgcta
     5341 aaaaataaac agcactaagg aaaactcagc ttttaagttc cttccttagt gaacagtcgc
     5401 ctaaatagag aaattttttt taaattttga ttttgaaatg aaaaatacaa aggaaggctt
     5461 ctaaacctct tcctttgtat tttcctttgt cttgcaagaa tctacaggct gagatttaaa
     5521 ctggctgctg accggcaaag gcttaccgta tcttgataaa gccaggctgg aaaacgctgg
     5581 agcattctct gtaagcagaa attctgatgg aattactgac actatgggca accctgtcga
     5641 cttgtcagag tttgtatttg tgccgcgcta caacgtagcc gcagcagcag ggtatggcgc
     5701 accggtcttc ggcgaagagc ctttgttctg cttggctttc cggaaatatt ggattgacaa
     5761 ctatgtgacc aaacagccgg aaaaactgtc tgtaattacc gttaaaggcg attcaatgga
     5821 gggcgtgctc aatcatgggg attccattct catcaatcac gccaaaaccg acccgcgtga
     5881 cggcctatat gtcttgcgta taggcaatga cctctttgtc aaacgtgtgc aacgcatacc
     5941 gggcaaactg cttgtaacgt ctgaaaatcc acgttacgca ccgtttgaaa tagattttag
     6001 caatgcccaa gacgacatcg ccatcatagg ccgtgttgag tggtatggcc ggagtattga
     6061 ttaaatccat tttaaaaacc tcttaaaaca gttttaaaaa ttccctaatc tcgtcagctt
     6121 taaacagaaa accgcgcatt ccggcgcggt tttgtgaaaa agctggcgta acttttccgg
     6181 atacaaaaaa acgccgaaat ccacgtcttt ccgagatttc agcgtttttt actctatttg
     6241 ttccttgtgc aaaaactaac agtcccccac aggaaaacgt cgctctgttc cgccacaacg
     6301 gcgaaatcct gttgagcggc atcaacatca aactcaaaag cggcgattcc ctgctgatac
     6361 gcggcccgag cggttgcggc aaaacttcgt tgctgcgcgc gctggctggg ctttggccgt
     6421 tcggcagcag cggcaaagtc agccgtccgc cgcatcaaga catcctcttc ctgccgcaac
     6481 gcccgtacac ggcacagggc agcctgcgcg acgcggtttg ttatcccgac atcgacaaac
     6541 agcatccgga gctaatcgaa gccatgaaca cctgccgctt gggttatctg attgacaaat
     6601 tggacaaaac agacgactgg cagcacaaac tctccctggg cgaactgcaa cgcgtcgcct
     6661 tcgtccgcgc cctgctttcc aagcccaaaa tcgtcctgct cgacgaagcc accgccgcct
     6721 tggacgaacc ggccgaagcc gcgctctacc gcgccttaaa gcaaaaactg cccgacagca
     6781 tcatcatcag catcggacac cgaagcacgc tcaacgcgtt ccacaatatg cgcctcgatg
     6841 tcggcaacgt tgcctgcggt tgaacgatgc cgtctgaagc cgttttcaga cggcatcgtt
     6901 caaattggaa aggcgcgcga aaacaacgta caataaaccc gtttaccacc cctcggaatg
     6961 ccgaaccgtg aaccagctta ttttcgactt tgccgcacac gactatccga gtttcgacaa
     7021 attcctcggc acggaaaacg cggaactggt ctatgtcctc cgacacaaac acggacagtt
     7081 tatctatgtc tggggtgaag aaggcgcggg caaaagccat cttttgcagg cgtgggtcgc
     7141 acaggcgctc gaagccggca aaaacgccgc ctatatcgat gccgcctcca tgccgctgac
     7201 cgatgccgcg ttcgaggcgg aatacctcgc cgtcgatcaa gtcgaaaaac tgggcaacga
     7261 agaacaagcc ctgctttttt ccatcttcaa ccgcttccgc aacagcggca aaggcttttt
     7321 gctgctcggt tcggaataca caccccagca gctcgtcatc cgcgaagatt tgcggacacg
     7381 tatggcgtac tgcctcgttt acgaagtcaa acccttaacc gaccaagaaa aaatcgacgc
     7441 gctcgccagt atggcggcgg cacgccaagt aaccgtcgat tccgaaattt tcgaatacct
     7501 gctcaaacac tggcggcgcg atatggacag cctgatgatg atgctcgaca cgctggacaa
     7561 ctacgccgtc acgatgggca aacgcatcac cctgccgctt ttgcgccagc ttttgaaaca
     7621 acaggaaacc caatgaaaaa cctcgccatc ttcgacctcg acaacaccct catcaacacc
     7681 gattccgacc acgcctggcc gcaatacctc atcaaaaaag gcttggttga cgctgccgaa
     7741 accgaagcgc aaaacgaaaa attctaccgc gactaccaaa acggctgcct cgacatcgac
     7801 gcattcctca aattccacct cgccccgctc gcccgttaca gcaaagaaga gctggcggaa
     7861 tttcaccgcg aatttatggc ggaatacatc atctcccaca tctcgcctat gcagcgtatg
     7921 ctggtgcaga gccaccaaat ggcaggcgac gaaaccctcg tgatttcctc aaccaacgag
     7981 ttcatcatca cccccatctg ccaccttttc ggcatcacca acatcatcgg cacacaactc
     8041 gaaaccgatc ccgacggtcg ctacaccggc aattacatcg gtacgcccag cctcaaagaa
     8101 ggcaaaatta cccgcctgaa ccaatggctt gccgaacgcg gcgaaacgct ccaaagctac
     8161 ggcaaaacct atttttacag cgactccaga aacgacctgc cgctgctgcg cctcgtcagc
     8221 gaacccgtca ccgtcaaccc cgatgccgag ctggaaaaag aagccaaaga aaaaggctgg
     8281 ccggttttaa atttcaaatg acgcaatgcc gtctgaagcc caattcggcg ttcagacggc
     8341 attgaggctg aaatccaaaa aactcaaagc cacaggaatt tatccggaaa aacaaaaaac
     8401 acaggaattt atccggaaaa acaaaaaaca caggaattca tcggggaaaa caacaatctt
     8461 tccaccgtca ttcccacgaa agtgggaatc ttgatccgtc cgcacggaaa cttatatttc
     8521 gtcatttgca cccgacataa ttcaatactt tattttgcaa taaattaacg gatgctgaca
     8581 aattagattg gattaattac cctattatct atattgggtt atcgggttgc taattgagca
     8641 gagcgtagca aaccataaag tgagtgtttc cacccactct gcatttatag tggattaaca
     8701 aaaatcagga caaggcgaca aagccgcaga cagtacagat agtacggcaa ggcgaggcaa
     8761 cgctgtactg gtttaaattt aatccactat actatgaagt ggttattaaa tatgataatg
     8821 agacctatta aatttagtat ggtaaatatg ttattattta ttgttatatg tagttcattt
     8881 tttgatctgc tcgttcaatt atgtacaatt ttatttcata gtcaaaaaat atactttatt
     8941 acattatttt tattatttat ttttaatttt gctacaaaat ctatctatat ggcaattatt
     9001 tatcctattt tatatttttt tacgataaaa aaatattatc cttactctag gaaagtgata
     9061 attctattat tattagcatt atctatatat tttagtttta tggactttta ctttttttcc
     9121 atatattcag ataaccttag ctatgaaacg gagcctttac atttatacat ccctattatt
     9181 attaattttt tctcactttt agtttctaat tttattttat cttttatcaa caagtaaatg
     9241 tatatacaaa atacaatcca aaagatccta agcaaaccac caatcccaca ccacccttac
     9301 cggttcaaat ccgcacaaaa acccacatcc cgtcattccc acgaaagtgg gaatctagga
     9361 cgtatagtgg attaacaaaa atcaggacaa ggcgacgaag ccgca
//
LOCUS       DAWFHY010000079         9091 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.079, whole genome shotgun sequence.
ACCESSION   DAWFHY010000079 DAWFHY010000000
VERSION     DAWFHY010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9091
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.079"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            317..790
                     /locus_tag="WGQ68_001685"
     CDS             317..790
                     /locus_tag="WGQ68_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0715451.1"
                     /translation="MTMQVFLTIPGYDVEAEIEKFVWMDAVIWQMPSWWMHEPWTVKK
                     YIDEVLTAGHGKLYQSDGRHSVNPTEGYGTGGLLQGKKHMLSLTWNAPIEAFTREGDF
                     FEGKGVDVLYMHFHKANEFLGMTRLPTFLCNDVVKNPQVEKYLADYQAHLEKVFG"
     gene            complement(998..2383)
                     /gene="pntB"
                     /locus_tag="WGQ68_001686"
     CDS             complement(998..2383)
                     /gene="pntB"
                     /locus_tag="WGQ68_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003676645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HEZ0715452.1"
                     /translation="MSSGLVTAAYIVAAILFIFSLAGLSKQETAKQGCYSGIAGMAVA
                     LFVTVFSDNTHGLGWIIIAMLIGAAIGIYKAKKVEMTEMPELIALLHSFVGLAAVLVG
                     FNSYIAPGNVSHDMHTIHLVEVYLGIFIGAVTFTGSLVAFGKLNGKISSSPLQLPAKH
                     KLNALALGVSFVLLLVFVGIDGSGFILLIMTLIALAFGWHLVASIGGADMPVVVSMLN
                     SYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFVSVIAGGFGSDSG
                     TSSSGSQEIGEYREVKAADVAEMLKSANSVIITPGYGMAVAQAQYPVAEITELLHKNG
                     TEVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFPETDVVLVIGANDTVN
                     PAAQTDPNSPIAGMPVLEVWKAKEVVVFKRSMNTGYAGVQNPLFFNENSVMCFGDAKK
                     TVDDILSELKK"
     gene            complement(2449..2832)
                     /locus_tag="WGQ68_001687"
     CDS             complement(2449..2832)
                     /locus_tag="WGQ68_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEZ0715453.1"
                     /translation="MTFAYWCILIACLLPLFCAAYAKKAGGFRFKDNHNPRDFLAHTQ
                     GTAARAHAAQQNGFEAFAPFAAAVLTAHATGNAAQSTINTLACLFILFRLAFIWCYIA
                     DKAAMRSLMWAGGFACTVGLFVAAA"
     gene            complement(2836..4377)
                     /locus_tag="WGQ68_001688"
     CDS             complement(2836..4377)
                     /locus_tag="WGQ68_001688"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HEZ0715454.1"
                     /translation="MKIGIPRESLSGETRVACTPATVALLGKLGFETVVESGAGLAAS
                     LDDAAYQTAGATVADKAAVWVCPLIYKVNAPSEQELPLLNEGQTIVSFLWPRQNEALV
                     EALRAKKVNALAMDMVPRISRAQALDALSSMANISGYRAVIEAANAFGRFFTGQITAA
                     GKVPPAQVLVIGAGVAGLAAIGTANSLGAVVRAFDTRLEVAEQIESMGGKFLKLDFPQ
                     ESGGSGDGYAKVMSDEFIAAEMKLFAEQAKEVDIIITTAAIPGKPAPKLITKEMVESM
                     KSGSVIVDLAAATGGNCELTRPGELSVTGNGVKIIGYTDMANRLAGQSSQLYATNLVN
                     LTKLLSPNKDGEITLDFEDVIIRNMTVTRDGEITFPPPPIQVSAQPQQTPSEKAAPAA
                     KPEPKPVPLWKKLAPAVIAAVLVLWVGAVAPAAFLNHFIVFVLACVIGYYVVWNVSHS
                     LHTPLMSVTNAISGIIVVGALLQIGQGNGFVSLLSFVAILIAGINIFGGFAVTRRMLN
                     MFKKG"
     gene            4667..5500
                     /gene="serB"
                     /locus_tag="WGQ68_001689"
     CDS             4667..5500
                     /gene="serB"
                     /locus_tag="WGQ68_001689"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HEZ0715455.1"
                     /translation="MPHALVLQFPSAEALPSDFPSRLPEPDYADEKRMRFIVEEGFSL
                     SGADAALLGSRQIDHAVLSDMDFGELGLIVSDMDSTLITIECVDEIAAGVGLKDKVAE
                     ITERSMRGELDFEQSLRSRVALLAGLDEQILADVYENVLKLSPGAEFLLDECKRHDVK
                     FLLVSGGFTFFTERLQQRLGFEYQHANVLEIENGRLTGRLKGRIIDAQAKADLLLEYR
                     SRLGLQPHQVLAMGDGANDIPMLKETGIGVAYRAKPKVRAAADVCINFGGLERVRGLF
                     R"
     gene            5566..6789
                     /locus_tag="WGQ68_001690"
     CDS             5566..6789
                     /locus_tag="WGQ68_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEZ0715456.1"
                     /translation="MGRRKLWFALAAAGVIGGLIGIVLTELMHFIQHTAYGYGADGVY
                     TSFREGVAQASGMRRVAVLTLCGAVAGGGWWLLKRFGKPQIEIKAALKQPLQGLPFLT
                     TVFHVLLQIITVGLGSPLGREVAPREMTAAFAFAGGRRLGLDEDEMRLLIACASGAGL
                     AAVYNVPLASTLFILEAMLGVWTQQAVAAALLTSVIATAVARIGLGDVQQYHPANLAV
                     NTSLLWFSAVIGPILGAAAVFFQRTAQKFPFIKRDNIKIIPLAVCMFVLIGVISVWFP
                     EVLGNGKAGNQLTFSGLTGWQHSLELTAVKWLVVLMALAAGAYGGLITPSMMLGSTIS
                     FAAAAAWNSVFPEMPSGSAAVVGAAVFLGVSLKMPLTAIVFVLELTYAPLSLLLPLCI
                     GMAGALGTSGKMGFK"
     gene            6950..8530
                     /gene="purH"
                     /locus_tag="WGQ68_001691"
     CDS             6950..8530
                     /gene="purH"
                     /locus_tag="WGQ68_001691"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEZ0715457.1"
                     /translation="MSVIKRALISLSDKAGAVEFAQTLTKLGVEILSTGGTAKLLADA
                     GVPVIEVADYTGFPEMLDGRVKTLHPKIHGGILGRRDLGEHVAKMEEHGIGNIDLVCV
                     NLYPFAATIAKPNCTLEDAIENIDIGGPTMVRSAAKNWKHVAIVTDTADFPAIAAELE
                     ANNGALSDKTRFNLSRKAFSHTAQYDGMISNYLTSLSDDVLSGEPQIGEFPSQFNQSW
                     IKVQDMRYGENPHQRAAFYRDVYPAAGSLSAYKQLQGKELSYNNIADADAAWEAVKSF
                     DAPACVIVKHANPCGVAVAADTLTAYKLAYATDTTSAFGGIIAFNREVDGETVKQITD
                     NQFMEVLMAPKFTAEALEIAAAKKNVRVLEVPLEAGANRFELKRVGGGLLVQTPDIHR
                     ISRADLKVVSKRQPTEQEWNDLLFVWNVAKYVKSNAIVFGKGGQTYGIGAGQMSRVDS
                     TRIAARKAQDAGLDLNGACAASDAFFPFRDGVDVIAEQGIKAIIHPAGSMRDQEVFDA
                     ADEHGIAMVVTDVRHFRH"
BASE COUNT         2072 a         2443 c         2552 g         2024 t
ORIGIN      
        1 tgcgcgggaa tgacaactca ttagttacct aaaacttaaa aagcagaaac ctttacgccg
       61 tcattcccac gaaagtggga atccgggaac ttaacgttac agtgatttat cggaaacggc
      121 tgaaaccgaa tgaattggat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa
      181 cctgcaccac gtcattccca cgaacctaca ttccgtcatt cccacgaaag tgggaatcca
      241 ggacgaaaaa tctccagaaa ccgttttacc cgataagttt ccgcaccgac aggtctggat
      301 tcccgcctgc gcgggaatga cgatgcaggt atttctgacg attcccggct atgatgttga
      361 ggcagaaatc gaaaaattcg tttggatgga tgctgtgatt tggcagatgc cgagctggtg
      421 gatgcacgag ccttggacag tgaaaaaata catagacgaa gtattaaccg ctggacacgg
      481 caaactctac caaagcgacg gcagacacag cgtcaatccg actgagggct acggcacagg
      541 cggcttgttg caaggcaaaa aacatatgct ttcactgact tggaatgcgc cgattgaggc
      601 gtttacccgc gaaggcgatt tctttgaagg caaaggcgtt gatgttttgt atatgcactt
      661 ccacaaagcc aacgagtttt tgggtatgac ccgcctgccg acattcttat gtaacgatgt
      721 ggttaaaaat ccgcaagtgg aaaaatactt ggcagattat caggcacact tggaaaaagt
      781 gttcggctaa ttaaaaatcc atcttcaaca cggagatgga ttttgtttgt ttcgttgatt
      841 ttgtgtcagt ttcagatgta ggtgcttatt cggacgggcc gtctgaaaat gtttgcccca
      901 atgcaaaaaa atcactgcaa accttcataa acggggtttg cagtgatttt ttcaaatcaa
      961 acagattgaa aacctgcgcc gaattgttca gacggcatta ttttttcagt tcggacagaa
     1021 tgtcatctac ggttttcttc gcatctccga aacacatcac gctgttttcg ttgaagaaca
     1081 gtgggttttg tacacctgcg tagccggtat tcatcgagcg tttgaagacg acgacttctt
     1141 ttgccttcca cacttccaac acgggcatac ccgcaatcgg gctgttcggg tcggtttggg
     1201 cggcaggatt gacggtatcg ttcgcaccaa tcaccaaaac cacatcggtc tcggggaagt
     1261 cgtcgttgat ttcgtccatt tccaaaacga tgtcgtaggg gactttggct tcggcgagca
     1321 gtacgttcat atgaccgggc aggcggccgg cgacggggtg gatgccgaag cgtacttctg
     1381 tgccgttttt atgcaaaagc tcggtgattt cggcaacggg gtattgcgcc tgtgcgactg
     1441 ccatgccgta gcccggggtg atgatgacgc tgtttgcgct tttcagcatt tcggcaacat
     1501 cggcagcttt gacttctcgg tattccccta tctcttggct gccggaagat gatgtgccgc
     1561 tgtcgctgcc gaaaccgccg gcaattaccg agacaaacga gcggttcatg gctttgcaca
     1621 taatgtagga cagaatcgcg ccgcttgagc caaccagcgc gccggtaacg atgagcaggt
     1681 cgttggagag cataaagcct gccgctgcgg ccgcccagcc ggagtaggag ttgagcatgg
     1741 acacgaccac gggcatatct gcgccgccga tggaggcaac caagtgccag ccgaatgcga
     1801 gggcaatcag ggtcataatc agcaggatga agccgctgcc gtcaatgccg acgaatacga
     1861 gcagcaacac aaacgatacg ccaagtgcca gtgcgttgag cttgtgtttg gcgggcagtt
     1921 gcagcgggct gctgctgatt ttgccgttga gcttgccgaa tgcgaccagc gagccggtaa
     1981 aggttaccgc gccgatgaaa atgcccaaat agacttcgac cagatggatg gtgtgcatat
     2041 cgtgcgaaac gttgcccggc gcgatatagc tgttgaagcc gaccaaaacc gccgctaggc
     2101 cgacgaagct gtgcagcagg gcaatcagtt cgggcatttc ggtcatttcc acctttttgg
     2161 ctttgtagat gccgattgcc gcgccgatga gcatggcgat gatgatccag cccagtccgt
     2221 gggtattgtc ggaaaaaaca gttacaaaaa gggcgaccgc cataccggcg ataccggaat
     2281 agcagccctg tttggcggtt tcctgtttgg acagccccgc cagtgagaag atgaataaaa
     2341 ttgcggcaac gatatacgcc gctgttacga gtcctgaaga catggaaatt ctccgatttt
     2401 cgatgatttg ttttcaatgc cgtccgaact gttcagacgg tatctgtttc aagcagccgc
     2461 gacaaacagc ccgacggtgc aggcaaatcc gcctgcccac atcagtgagc gcatagcggc
     2521 tttgtcggcg atatagcacc agataaaggc gaggcggaac aggatgaaca ggcaggcaag
     2581 cgtgttgatg gtcgattgcg ccgcattgcc ggttgcgtgt gccgtcaaaa cggcggcggc
     2641 aaacggtgca aaggcttcaa aaccgttttg ctgcgcggcg tgggcacggg cggctgtgcc
     2701 ttgcgtgtgc gccagaaaat cgcgcggatt gtggttgtct ttaaaccgga atccgcccgc
     2761 ttttttggca tatgccgcac aaaaaagcgg caataggcag gcaatcagga tgcaccaata
     2821 ggcgaaagtc atggcttacc ctttcttaaa catattcagc atacgccgtg taaccgcaaa
     2881 gccgccgaag atgttgatgc cggcaatcag gatggcaaca aacgacagca gcgaaacaaa
     2941 gccgttgccc tgaccgattt gcagcagcgc gccgacgacg atgatgccgg agatggcgtt
     3001 ggtcacggac atcagcggcg tatgcagcga gtggctgaca ttccaaacga cgtagtaccc
     3061 gatgacgcag gcgaggacga acacgataaa gtggttcaag aatgctgcgg gtgcgaccgc
     3121 gccgacccac agcactaata cggcggcgat gacggcaggc gcgagttttt tccacagggg
     3181 aacgggtttc ggctcgggct tggcggcagg cgcggctttt tcagacggcg tttgctgcgg
     3241 ctgggcggaa acttgaatcg gcggaggcgg gaaggtgatt tcgccgtcgc gggtaacggt
     3301 catgttacgg ataatcacgt cttcaaaatc cagcgtgatt tcgccgtctt tgttcgggct
     3361 taacagcttg gtcaggttca ccaagttggt ggcgtaaagc tgggaagact gtccggcaag
     3421 gcggtttgcc atgtcggtgt agccgatgat tttcacgccg ttgccggtta cggacaattc
     3481 gcccgggcgg gtgagttcgc agttgccgcc cgtcgccgcc gccaaatcga cgatgacgga
     3541 gccggatttc atgctttcca ccatttcttt ggtaatcagc ttgggcgcgg gtttgcccgg
     3601 aatggcggcg gtagtgatga tgatgtccac ttctttcgcc tgctcggcaa agagtttcat
     3661 ctcggcggcg ataaattcgt cgctcatcac tttggcgtag ccgtcgccgc tgccgcccga
     3721 ttcttgcggg aagtcgagtt tcaggaactt gccgcccatc gattcgattt gttccgccac
     3781 ttccaagcgg gtatcgaacg cgcgcaccac tgcgcctagt gaatttgccg taccgattgc
     3841 cgccaaacct gccacacctg caccaatcac caaaacctgc gcaggcggca ctttgccggc
     3901 ggcagtaatt tgaccggtga agaaacggcc gaaggcgttg gcggcttcaa ttacggcgcg
     3961 gtagccgctg atgtttgcca tcgaagacaa agcgtccaaa gcctgcgcgc gcgaaatgcg
     4021 gggcaccata tccatcgcca gcgcgttcac tttcttggcg cgcaaggctt cgaccaaagc
     4081 ctcgttttgg cgcggccaca ggaagctgac gatggtttga ccttcgttca aaagcggcag
     4141 ttcctgttcg gacggcgcgt tgaccttata aatcaaaggg cagacccaaa ccgccgcttt
     4201 gtcggcaacg gttgcgcctg ctgtttggta agcggcatcg tccaaacttg ccgccaaacc
     4261 tgcaccgctt tcgacaacgg tttcaaagcc cagtttgccc agcagggcga cggtggcggg
     4321 cgtacaggcg acgcgggttt cgccggataa tgactcgcgt gggataccga ttttcatctc
     4381 tgaatccttt ttcgggttgt ttatatgtat cgtgggttaa atttaaatcg gggcggggcg
     4441 gagcaacgcc gtaccggttt aaagccgact cacttcaaat gttaatatat tttagataat
     4501 ccccttataa cgaattttca tcaggctggc aatagttgcg gcattttccc gtgttgtccg
     4561 acacatactg ccgtttgtgt ttgaagtcgt tttccgattg cagtcccgcc cgccgtaaca
     4621 ttctttgccg tttcactata taatccgcat tttttgagcc gccgttatgc cgcacgccct
     4681 cgtcctccaa tttccctccg ccgaagccct gccttccgac ttcccctcac gcctgcccga
     4741 acctgattac gccgatgaaa agcgtatgcg ttttatcgtt gaagaagggt tttctttaag
     4801 cggggcggac gcggcgttgc ttggcagccg tcaaatcgac cacgccgtgt tgtcggatat
     4861 ggattttggc gaactcggtt tgattgtcag cgatatggat tcgacgctga ttaccatcga
     4921 atgcgtcgat gaaattgcgg caggcgtggg tttaaaagac aaggtagcgg aaattaccga
     4981 gcgttcgatg cgcggagagt tggatttcga gcagtcgttg cgcagccgcg ttgcgctgtt
     5041 ggcgggattg gacgaacaga ttttggcgga cgtttatgaa aacgttttga agctctcgcc
     5101 cggtgcggaa tttttgttgg acgaatgcaa aaggcacgat gtgaaattcc tgctggtgtc
     5161 gggcggcttc acgtttttta ccgaaaggct gcaacaacgc ctcggcttcg aataccagca
     5221 cgccaatgtt ttggaaattg aaaacggcag gctgaccgga cgtttgaaag gcagaatcat
     5281 cgacgcgcag gcaaaggcag atttattgct cgaataccgc agccgcctcg gattgcagcc
     5341 acatcaggtg ttggcgatgg gcgacggtgc gaacgatatt ccgatgctca aagaaacggg
     5401 cataggcgtg gcttaccgtg ccaaaccgaa agtgcgggca gccgccgatg tctgtatcaa
     5461 cttcggcggt ttggagcgtg tacgcggcct gttcaggtag gcggatagga aacggatgtc
     5521 gtctgaaaaa cgtgttcaga cggcatttga acggcaggaa cgacagtggg acgcagaaaa
     5581 ctttggtttg ccctggcagc agcaggcgtt atcggcggtc tgatcggcat tgtgctgacg
     5641 gaactgatgc acttcataca gcatacggca tacggttatg gcgcggacgg cgtgtacact
     5701 tcgttccgcg aaggcgtggc acaggcttcc ggtatgcggc gcgttgccgt gctgacgctg
     5761 tgcggcgcgg tcgcaggcgg cggctggtgg ttgctcaaac gtttcggcaa gccgcaaatc
     5821 gaaatcaaag ccgccttgaa acagccgttg caggggctgc cgtttctgac gacggttttc
     5881 catgttctgc tgcaaatcat aacggtcgga ctcggttcgc cgctcggacg cgaagtcgcg
     5941 ccgcgtgaaa tgactgccgc gtttgctttt gccggcggca ggcggttggg tttggatgaa
     6001 gacgaaatgc gcctcttgat tgcctgtgcg tccggcgcgg gtttggcagc cgtgtataat
     6061 gtgccgctcg cctccacact tttcatcctc gaagccatgc tgggcgtgtg gacgcagcag
     6121 gcggttgccg ccgcgttgct gacttccgtc attgccaccg ccgtggcgcg tatcggctta
     6181 ggcgacgtac agcagtatca tcccgccaac cttgccgtaa atacttcatt gctttggttt
     6241 tccgccgtca tcggcccgat attgggcgca gccgccgtct ttttccagcg taccgcccaa
     6301 aagttcccct ttatcaagcg cgacaacatc aaaattattc ccttggcagt ctgcatgttt
     6361 gtgctcatcg gcgtgatttc cgtttggttt cccgaagttt tgggcaacgg caaagcaggc
     6421 aaccaactga cttttagtgg tttgaccggt tggcaacaca gccttgagct gaccgccgtc
     6481 aaatggctgg ttgtcttgat ggcgttggcg gcaggcgcat acggcggact gattacgccg
     6541 tctatgatgc tcggcagcac catttctttt gcagccgccg ccgcgtggaa cagcgttttc
     6601 cccgaaatgc cgtctggaag cgcggcagtc gtcggagcgg cggtgtttct cggcgtgtcg
     6661 ctcaaaatgc cgctgaccgc cattgtgttc gtattggaac tgacctacgc gccgctgtcc
     6721 ctgctgctgc ctttgtgcat cggcatggcg ggggcattgg gaacatcggg aaaaatgggc
     6781 ttcaaatgaa tgccgacaaa tgccgtctga aaggttttca gacggcattt ctttgtaaat
     6841 tgtcagcaat atctgcaaaa acctactgcc aaaaaactag aaatagcgga taataccgcc
     6901 ccgaaaaagt tcccctttcc ccatccaact gtcagaaaag gaagccccaa tgtccgtcat
     6961 caaacgcgcc ctgatcagcc tgtccgacaa ggcaggcgcg gtcgaatttg cccaaaccct
     7021 gaccaagctc ggtgttgaaa tcctctctac cggcggtaca gcaaagctct tggctgatgc
     7081 aggcgttccc gttatcgaag ttgccgacta taccggcttc cccgaaatgc tcgatggccg
     7141 cgtgaaaacc ctgcatccga aaatccacgg cggcatcctc ggtcgtcgcg atttgggcga
     7201 acacgtcgcc aagatggaag aacacggtat cggcaacatc gacctcgtgt gcgtcaacct
     7261 ttaccccttc gccgccacca tcgccaaacc aaactgcaca ctggaagacg cgattgaaaa
     7321 catcgacatc ggcggcccga ctatggtgcg ttctgccgcg aaaaactgga aacacgtcgc
     7381 catcgttacc gataccgccg atttccctgc catcgccgcc gaactcgaag ccaacaacgg
     7441 cgcattgagc gacaaaaccc gtttcaacct ctcacgcaaa gcattcagcc ataccgccca
     7501 atacgacggc atgatttcca actacctgac ctcgctttca gacgacgtct tgagcggaga
     7561 gccccaaatc ggcgaattcc cgagccagtt caaccaaagc tggattaaag tgcaagatat
     7621 gcgctacggc gagaatccgc accaacgcgc cgcgttctac cgcgatgtgt accccgccgc
     7681 aggcagcctt tccgcctaca aacagctcca aggtaaagaa ttgtcgtaca acaacatcgc
     7741 cgatgccgat gccgcatggg aagccgtcaa atcctttgac gcgcccgcct gcgtgattgt
     7801 gaagcacgcc aatccgtgcg gcgtcgccgt tgcagccgat accttgaccg cctacaaact
     7861 cgcctacgcc accgacacca ccagcgcgtt cggcggcatc atcgccttca accgcgaagt
     7921 ggacggcgaa accgtcaaac agattacaga caaccagttt atggaagtcc tgatggcgcc
     7981 gaagttcacc gccgaagccc tcgaaatcgc cgccgccaag aaaaacgtgc gcgtgttgga
     8041 agtgcctttg gaagcaggcg cgaaccgctt tgaactcaaa cgcgtcggcg gcggattgtt
     8101 ggtacaaacg cccgacatcc accgcatcag ccgcgccgat ttgaaagtcg tctccaaacg
     8161 ccaaccgacc gaacaggaat ggaacgattt gctgttcgtt tggaacgtcg caaaatacgt
     8221 caaatccaac gccatcgtct tcggcaaagg cggtcaaacc tacggcatcg gcgcaggcca
     8281 aatgagccgc gtggacagta cccgcatcgc cgcccgcaaa gcgcaggacg caggcttgga
     8341 cttaaacggc gcatgtgccg cctctgatgc tttcttcccc ttccgcgacg gcgtggacgt
     8401 gattgccgag cagggcatca aagccatcat ccatccggca ggttcgatgc gcgatcagga
     8461 agtcttcgac gcagcggacg aacacggcat cgccatggtc gtaaccgacg tgcgccactt
     8521 ccgccattaa cgcagatgaa caaggcaatg ccgtctgaag ggctttcaga cggcattttg
     8581 cgctattttg tgaaggtagg gatgacggtt cgggtattcc tgacagggtg gattttcaag
     8641 gtgttgtata gggtgtagga ggattcgtaa aaggtgggat gcagggtgtg cttcagcccg
     8701 ctgcatcaaa aatttttgga gaatcggcgg gagtcggcgg ttttggtttc ggcggggacg
     8761 gtggaaatgg gtaacattga cggaatcgac ggaagcggtg ggcttcagcc caccgcttgt
     8821 atattggaat caccgtatca tagcgccacc cgcgcccacc caaggcacac aaccgttgcg
     8881 tagctcaggg agcggcaggg caacccatcg acacaaccgg acagttgccg gacaacacaa
     8941 ccgaatgcaa ggcaggttga tgatgagtac ccgataccat tacgcaggta tagtgaatta
     9001 acaaaaacca gtacgacgtt gcctcgcctt agctcaaaga gaacgattct ctaaggtgct
     9061 gaagcaccaa gtgagtcggt tccgtactat c
//
LOCUS       DAWFHY010000080         9071 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.080, whole genome shotgun sequence.
ACCESSION   DAWFHY010000080 DAWFHY010000000
VERSION     DAWFHY010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.080"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(128..463)
                     /locus_tag="WGQ68_001692"
     CDS             complement(128..463)
                     /locus_tag="WGQ68_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003699221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEZ0715458.1"
                     /translation="MFKDWKEHTALVKKSFGELGKAHPKMLQAYGALEQAAAAEALDA
                     KTRELIAIAVAITTRCESCISVHAAAAAKAGATDSEIAGALATAIALNAGAAYTYALR
                     ALEAVETQK"
     gene            592..1497
                     /gene="mtrA"
                     /locus_tag="WGQ68_001693"
     CDS             592..1497
                     /gene="mtrA"
                     /locus_tag="WGQ68_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter MtrCDE transcriptional
                     activator MtrA"
                     /protein_id="HEZ0715459.1"
                     /translation="MDILDKLVDFAQLTGSVDVQCLLGGQWSVRHETLQREGLVHIVT
                     SGSGYLCIDGETSPRPVSTGDIVFFPRGLGHVLSHDGKCGESLQPDMRQHGAFTVKQC
                     GNGQDMSLFCARFRYDTHADLMNGLPETVFLNIAHPSLQYVVSMLQLESKKPLTGTVS
                     VVNALSSVLLVLILRAYLEQDKDVELSGVLKGWQDKRLGHLIQKVIDKPEDEWNVDKM
                     VAAANMSRAQLMRRFKSRVGLSPHAFVNHIRLQKGALLLKKNPDSVLSVALSVGFQSE
                     THFGKAFKRQYHVSPGQYRKEGGQK"
     gene            complement(1559..2047)
                     /locus_tag="WGQ68_001694"
     CDS             complement(1559..2047)
                     /locus_tag="WGQ68_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEZ0715460.1"
                     /translation="MKKYLIPLSIAAVLSGCQSIYVPTLTEIPVNPINTVKTEAPAKG
                     FRLASSHWTDVAKISDEATRLGYQVGIGKMTKVQAAQYLNNFRKRLVGRNAVDDSMYE
                     IYLRSAIDSQRGAINTEQSKLYIQNALRGWQQRWKNMDVKPNNPAFTNFLMEVMKMQP
                     LK"
     gene            2194..3096
                     /locus_tag="WGQ68_001695"
     CDS             2194..3096
                     /locus_tag="WGQ68_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0715461.1"
                     /translation="MDTAKKDILGSGWMLVAAACFTIMNVLIKEASAKFALGSGELVF
                     WRMLFSTVALGAAAVLRRDTFRTPHWKNHLNRSMVGTGAMLLLFYAVTHLPLATGVTL
                     SYTSSIFLAVFSFLILKERISVYTQAVLLLGFAGVVLLLNPSFRSGQETAALAGLAGG
                     AMSGWAYLKVRELSLAGEPGWRVVFYLSVTGVAMSSVWATLTGWHTLSFPSAVYLSCI
                     GVSALIAQLSMTRAYKVGDKFTVASLSYMTVVFSALSAAFFLGEELFWQEILGMCIII
                     LSGILSSIRPTAFKQRLQSLFRQR"
     gene            complement(3137..4267)
                     /locus_tag="WGQ68_001696"
     CDS             complement(3137..4267)
                     /locus_tag="WGQ68_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0715462.1"
                     /translation="MAKHLPLAVLTALLLAACGGSDKPSAENPTSAENRNVLKIYNWS
                     EYVDPETVADFEKKNGIKVTYDVYDSDETLESKVLTGKSGYDIVAPSNAFVGRQIKAG
                     AYQKIDKSLIPNYKHLNPEMMRLMDGVDPGHEYAVPFYWGTNTFAINTERVKKALGTD
                     KLPDNQWDLVFDPEYTSKLKQCGISYLDSAAEIYPMVLNYLGKNPNSSNTEDIREATA
                     LLKKNRPNIKRFTSSGFIDDLARGDTCVTIGFGGDLNIAKRRAEEAGGKEKIRVMMPK
                     EGVGIWVDSFVIPKDAKNVANAHKYINDFLDPEVSAKNGNFVTYAPSSKPARELMEDE
                     FKNDNTIFPTEEDLKNSFIMVPIQPAALKFMVRQWQDVKAGK"
     gene            4472..7096
                     /gene="alaS"
                     /locus_tag="WGQ68_001697"
     CDS             4472..7096
                     /gene="alaS"
                     /locus_tag="WGQ68_001697"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEZ0715463.1"
                     /translation="MKTSELRQKFLKFFETKGHTVVRSSSLVPHDDPTLLFTNAGMNQ
                     FKDVFLGFDKRPYSRATTAQKCVRAGGKHNDLENVGYTARHHTFFEMMGNFSFGDYFK
                     RDAIHFAWEFLTSPEWLNIPKDKLLATVYAEDDEAYNIWLNEIGMPSERIVRIGDNKG
                     AKYVSDNFWQMGDTGPCGPCSEIFYDHGEEIWGGIPGSPEEDGDRWIEIWNCVFMQFN
                     RDEQGNMNPLPKPSVDTGMGLERMAAVMQHVHSNYEIDLFQDLLKAVARETGAAFSMD
                     EPSLKVIADHIRSCSFLIADGVLPSNEGRGYVLRRIIRRAVRHGYKLGQSKPFFHKLV
                     ADLVKEMGDAYPELKEKQAQIEEALKNEESRFAQTLETGMALLENALAKGGKTLDGEI
                     IFKLYDTYGFPYDLTADMARELGIELDEAGFEREMEAQRARARAAQSFKANAQLPYDG
                     QDTEFKGYSERQTESKVLALYKDGEQVDELNEGDSGAVVIDFTPFYAESGGQVGDVGY
                     IFSGENRFEVRDTQKIKAAVFGQFGVQTSGRLKVGDSVTAKVDDEIRNANMRNHSATH
                     LMHKALRDVLGEHVEQKGSLVTAESTRFDISHPQAVTAEEIAEVERRVNEAILANVAV
                     NAAIMSMEDAQKTGAMMLFGEKYGDEVRVLQMGGFSTELCGGTHVSRTGDIGLFKIIS
                     EGGIAAGVRRIEAITGLNALKWAQEQERLVKDIIAETKAQTEKDVLAKIQAGAAHAKA
                     LEKELAKAKAELAVHAGAKLLDDAKDLGAAKLVAAQIEADAAALREIVTDLTGKSDNA
                     VILLAAVNDGKVSLCAGVSKPLTGKVKAGDLVKFAAEQVGGKGGGRPDLAQAGGTDAG
                     KLPEMLVSVESWLCQKLS"
     gene            7158..7889
                     /locus_tag="WGQ68_001698"
     CDS             7158..7889
                     /locus_tag="WGQ68_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715464.1"
                     /translation="MNTKTELQKLLEEDISTLTETLICADALPPRYVRSIATPIVRRW
                     LIDKQLNILAKEIGLTIELPILDTSLVFEKLSTLENKVNFYMAGGVYLGGEFISSIYH
                     SSQEFSGEPIIYAEPNIILCPAEKFLTLKRVFHNGNIFNMNQIITFLSNKQGGVHFDK
                     NYDKYKTWQVAIEKAANFLKLGNPYNEDKLSLSEEHDTILVVLPLEKGYEWNCLEIEV
                     LSAAQSLANIYCNKVRLIDGHVWKE"
     gene            8198..>9071
                     /locus_tag="WGQ68_001699"
     CDS             8198..>9071
                     /locus_tag="WGQ68_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent DNA methyltransferase"
                     /protein_id="HEZ0715465.1"
                     /translation="MKTLLQLQTAAQNFAAYYKDQTDERREKDTFWNEFFAIFGIDRK
                     NVAHFEYPVKDPADNTQFVDIFWEGIFLAEHKSANKNLTKAKEQAERYLQEIGRTKPS
                     ALPEYYAVSDFAHFHLYRRVPEEGAENQWQFPLEELPEYITRGVFDFMFGIEAKVRQI
                     QEEANIQAAAAIGRLHDALKEEGIYEEHELRLFITRLLFLFFADDSAVFQRNYLFQDF
                     LENCKEADTLGDKLNQLFEFLNTPDQKRSKTQSEKFKGFEYVNGGLFKERLRTFDFTA
                     KQHRALIDCGNFDWR"
BASE COUNT         2307 a         2385 c         2194 g         2185 t
ORIGIN      
        1 cttacccctt gtttctcaaa tgccgtctga aataagcggc ttaatatatt gtttacagta
       61 ttgggaagca taacaggcaa aatgccgtct gaaatatttt cagacggcat ttcttatccg
      121 aaacggatta tttttgcgtt tcaaccgctt ccaatgcacg cagggcgtaa gtgtaagcgg
      181 caccggcatt cagggcgatg gcggttgcca atgcgcctgc gatttcgctg tcggtcgcac
      241 cggctttggc ggcggcggcg gcgtgaacac tgatgcagct ctcacaacgt gtggtaatgg
      301 caacggcgat ggcaatcagt tcgcgcgttt tggcatcaag tgcttcggcg gcggcggcct
      361 gttccaatgc gccgtaggcc tgcagcattt taggatgcgc cttacccagc tcgccgaacg
      421 attttttaac caatgcggta tgttctttcc aatctttaaa cattttcttt tcctttctct
      481 tgcgtttaac cctgatacgc gcttgcgtat ctgttttcga tgtgcgtatt attgcaatta
      541 ttcagttgtg tttctcgttt aatcatctca ttttatggtt caaaaagatt tatggacatt
      601 ctggacaaac tggtcgattt cgcccaattg acgggcagtg tggatgtgca gtgccttttg
      661 ggcggacaat ggtcggtacg gcatgaaacc ttgcaacgcg aaggattggt acacattgtt
      721 acatcgggca gcggctatct ctgcatcgac ggcgaaactt ccccgcgtcc ggtcagtaca
      781 ggggatattg tatttttccc gcgcggcttg ggtcatgtgt tgagccacga cggaaaatgc
      841 ggagaaagtt tacaaccgga tatgcggcag cacggtgcgt ttacggtcaa gcagtgcggc
      901 aacggacagg atatgagcct gttttgcgcc cgtttccgct acgacaccca cgccgatttg
      961 atgaacgggc tgcctgaaac cgtttttctg aacattgccc atccgagttt acagtatgtg
     1021 gtttcaatgc tgcaactgga aagcaaaaaa cctttgacgg ggacggtttc cgtggtcaac
     1081 gcattgtcgt ccgtcctgct ggtgcttatc ctgcgcgcct atctcgaaca ggataaggat
     1141 gtcgaactct cgggcgtatt gaaaggttgg caggacaaac gtttgggaca tttaatccaa
     1201 aaggtgatag acaaaccgga agacgaatgg aatgtcgaca aaatggtggc ggctgccaat
     1261 atgtcgcgcg cgcaactgat gcgccgtttc aaaagccggg tcggactcag cccgcacgcc
     1321 tttgtgaacc atatccgcct gcaaaaaggc gcgttgctgc tgaaaaaaaa cccggattcg
     1381 gttttgtcgg tcgcactgtc ggtaggcttt cagtcggaaa cgcacttcgg caaggcgttc
     1441 aaacggcaat atcacgtttc gccgggtcaa taccggaaag aaggcgggca aaaataaatc
     1501 ggggcttcaa acgcaaatgc cgtctgaaaa ggctttcata cagcatttgc gtaccgcgtc
     1561 atttcaaggg ctgcatcttc atcacttcca tcaaaaagtt ggtaaatgcg gggttgttgg
     1621 gtttgacatc catatttttc caacgctgct gccagccgcg caaggcattc tggatataca
     1681 gcttggactg ttccgtattg attgcgcccc gctggctgtc tatcgccgaa cgcaggtaga
     1741 tttcatacat actgtcatcg acggcattgc gtccgaccag gcgttttctg aagttgttca
     1801 gatattgcgc cgcctgaacc ttggtcattt taccgatacc cacctgatag cccaagcgcg
     1861 tcgcttcatc gctgattttg gcaacatccg tccaatgcga agaggcaagg cggaaacctt
     1921 ttgcaggtgc ttccgttttg acggtattga taggattcac ggggatttcc gtcaatgtgg
     1981 gcacataaat agactggcag ccggaaagaa ctgccgcaat ggaaagaggg ataaggtatt
     2041 ttttcatgcc cccattataa tcaagtttgc cttgagaaaa caaattgttc ggcaagaaaa
     2101 ataaaatttc ggcatcagaa gcaggcaaaa acacattcca caagccttgc cgcaaggttt
     2161 acaatccgac cgtccttatc gcaacgaccg tttatggata ccgcaaaaaa agacatttta
     2221 ggatcgggct ggatgctggt ggcggcggcc tgctttacca ttatgaacgt attgattaaa
     2281 gaggcatcgg caaaatttgc cctcggcagc ggcgaattgg tcttttggcg catgctgttt
     2341 tcaaccgttg cgctcggggc tgccgccgta ttgcgtcggg acaccttccg cacgccccat
     2401 tggaaaaacc acttaaaccg cagtatggtc gggacggggg cgatgctgct gctgttttac
     2461 gcggtaacgc atctgccttt ggccaccggc gttaccctga gttacacctc gtcgattttt
     2521 ttggcggtat tttccttcct gattttgaaa gaacggattt ccgtttacac gcaggcggtg
     2581 ctgctccttg gttttgccgg cgtggtattg ctgcttaatc cctcgttccg cagcggtcag
     2641 gaaacggcgg cactcgccgg gctggcgggc ggcgcgatgt ccggctgggc gtatttgaaa
     2701 gtgcgcgaac tgtctttggc gggcgaaccc ggctggcgcg tcgtgtttta cctttccgtg
     2761 acaggtgtgg cgatgtcgtc ggtttgggcg acgctgaccg gctggcacac cctgtccttt
     2821 ccatcggcag tttatctgtc gtgcatcggc gtgtccgcgc tgattgccca actgtcgatg
     2881 acgcgcgcct acaaagtcgg cgacaaattc acggttgcct cgctttccta tatgaccgtc
     2941 gttttttccg ccctgtctgc cgcatttttt ctgggcgaag agcttttctg gcaggaaata
     3001 ctcggtatgt gcatcatcat cctcagcggt attttgagca gcatccgccc cactgccttc
     3061 aaacagcggc tgcaatccct gttccgccaa agataaaaaa tgccgtccga acatccttca
     3121 gacggcatat cgggctttat ttccccgcct tcacatcctg ccactggcgc accataaact
     3181 tcaatgccgc cggctggata ggcaccatga taaagctgtt tttcaaatcc tcctcggttg
     3241 ggaaaatcgt attgtcgttt ttaaattcgt cttccatcag ctcacgcgcc ggcttgctcg
     3301 aaggcgcata agtaacgaaa ttgccgtttt tcgccgacac ttccgggtcg aggaagtcgt
     3361 tgatgtattt gtgcgcgttg gcgacgtttt tcgcatcttt cggaatcacg aaagaatcca
     3421 cccaaatccc cacgccctct ttgggcatca tcacgcggat tttttccttg ccgcccgctt
     3481 cttcggcacg gcgtttggcg atgttcaaat cgccgccgaa accgattgtt acgcaggtat
     3541 cgccgcgcgc caaatcatcg ataaagccgg acgaagtgaa gcgtttgata ttggggcggt
     3601 ttttcttgag cagggcggtt gcctccctga tgtcttccgt attgctgctg ttcgggtttt
     3661 tacccaaata gttcaacacc ataggataga tttccgccgc gctgtccaaa tagctgatgc
     3721 cgcattgctt gagtttggac gtgtattcgg ggtcgaacac caaatcccac tggttgtccg
     3781 gcagcttgtc cgtacccaaa gcctttttca cgcgttcggt attgatggcg aaggtatttg
     3841 tcccccaata aaacggcacg gcgtattcgt ggccgggatc gaccccgtcc atcagcctca
     3901 tcatttcggg gttgaggtgt ttataattgg gaatcagcga cttatcgatt ttctgatacg
     3961 cacctgcctt aatctgcctg cccacaaacg cattggacgg cgcgacaatg tcgtaaccgg
     4021 acttgcctgt cagcaccttg ctttccagcg tttcatcgct gtcgtacaca tcataagtaa
     4081 ccttgatgcc gtttttcttt tcaaaatcgg caacggtttc cggatcgaca tattccgacc
     4141 agttgtaaat ttttaatacg tttcggtttt ccgccgatgt cggattttcg gcagatggtt
     4201 tgtccgaacc gccgcacgct gcaagcagca aagcagtcag gacggcgagt ggcagatgtt
     4261 tggccattat cattccttgc atatcgggtt ggagaaagcg gtcattatag ccgatattgg
     4321 caacagggct tcagacggca ttcaaaatcc cgccacaccc ttccgaaaac cgccgcttcc
     4381 atagctagaa acagggattt gcggtaagat accgccgttc gttttccctg cttttaccat
     4441 gacaagacat ttgagagaca ttgaaaaaat tatgaaaacc tccgaactgc gccaaaaatt
     4501 cctaaaattt tttgaaacca aaggccacac cgtcgtccgc tcttccagcc tcgtgccgca
     4561 cgacgacccg accctgctgt ttaccaacgc gggcatgaac cagtttaaag acgtattctt
     4621 aggtttcgac aaacgcccgt acagccgcgc caccaccgcg caaaaatgcg tacgcgccgg
     4681 cggcaaacac aacgacttgg aaaacgtcgg ctacaccgcc cgccaccaca ccttctttga
     4741 aatgatgggc aacttctcct tcggcgacta cttcaaacgc gacgccatcc atttcgcttg
     4801 ggagtttctg acttcccccg aatggctcaa catccctaaa gacaaactgt tggcgaccgt
     4861 ttacgcggaa gacgacgaag cctacaacat ctggttgaac gaaatcggta tgccgtccga
     4921 gcgcatcgtc cgcatcggcg acaacaaagg cgcgaaatac gtgtccgaca acttctggca
     4981 aatgggcgac accggccctt gcggcccctg ctccgaaatt ttctacgacc acggcgaaga
     5041 aatctggggc ggcattcccg gcagccccga agaagacggc gaccgctgga tcgaaatttg
     5101 gaactgcgta tttatgcagt tcaaccgcga cgaacaaggc aatatgaatc cgctgcccaa
     5161 gccgtccgtc gataccggta tgggcttgga acgtatggcc gccgtcatgc agcatgttca
     5221 cagcaactac gaaatcgact tgttccaaga cctgctcaaa gccgttgccc gcgaaaccgg
     5281 cgcagctttc agcatggatg aacccagcct gaaagtcatc gccgaccaca tccgctcctg
     5341 ctcgttcttg attgcagacg gcgtgttgcc ttccaacgaa ggccgcggct acgtattgcg
     5401 ccgcatcatc cgccgcgccg tgcgccacgg ttacaaactg ggtcaaagca aaccgttctt
     5461 ccacaaactg gtggccgatt tggtcaaaga gatgggcgat gcctaccctg agttgaaaga
     5521 gaaacaagca caaatcgaag aagcgttgaa aaacgaagaa agccgttttg cccaaacgct
     5581 ggaaactggt atggctttgt tggaaaacgc gctggccaaa ggcggcaaaa cactcgacgg
     5641 cgaaatcatc ttcaaactct acgacaccta cggtttcccc tacgacctga ctgccgacat
     5701 ggcgcgcgaa ctgggcatcg aactggacga agcaggcttc gagcgcgaaa tggaagccca
     5761 acgcgcacgc gcacgcgccg cccaaagctt caaagccaac gcccaactgc cttatgacgg
     5821 tcaagacacc gagtttaaag gttatagcga acgccaaacc gaatccaaag tcctcgccct
     5881 ctacaaagac ggcgagcaag tcgacgaatt gaacgaaggc gacagcggcg cagtcgtcat
     5941 cgactttacc ccgttctatg cagaatccgg cggccaagtc ggcgacgtcg gctatatctt
     6001 ctcaggcgaa aaccgctttg aagtacgcga tacccaaaaa atcaaagcag ccgtattcgg
     6061 acaattcggc gtacaaactt caggccgtct gaaagtcggc gacagcgtta ccgccaaagt
     6121 ggacgacgaa atccgcaatg ccaatatgcg caaccacagc gcgacccacc tgatgcacaa
     6181 agccctgcgc gacgtattgg gcgaacacgt cgaacaaaaa ggctctttgg ttaccgccga
     6241 atccacccgt ttcgacattt cccatcccca agcggtaact gccgaagaaa ttgccgaagt
     6301 agaacgccga gtcaacgaag ccatcttggc gaacgttgcc gtcaacgccg ccattatgag
     6361 catggaagat gcacaaaaaa ccggcgcgat gatgcttttc ggcgaaaaat acggcgacga
     6421 agtgcgcgta ctgcaaatgg gcggtttctc aaccgaattg tgcggcggca cgcacgtttc
     6481 acgcaccggc gacatcggcc tcttcaaaat catcagcgaa ggcggtattg ccgcaggcgt
     6541 gcgccgtatc gaagccatca ccggcctgaa cgcactcaaa tgggcgcaag agcaagagcg
     6601 tttggtgaaa gacatcattg ctgaaaccaa agcccaaacc gaaaaagacg tactggcgaa
     6661 aatccaagca ggcgcggcac acgccaaagc cttggaaaaa gagttggcaa aagccaaagc
     6721 cgaactcgcc gtccacgcag gcgcaaaact cttggacgac gcaaaagact tgggcgcagc
     6781 caaactcgtt gctgcccaaa tcgaagccga cgcagccgcc ctgcgcgaaa tcgttaccga
     6841 tttaaccggt aaatccgaca acgccgtgat tcttttagcg gcagtaaacg acggcaaagt
     6901 ctccctgtgc gccggcgtat ccaaaccgtt gaccggcaaa gtgaaagcag gcgatctggt
     6961 taaatttgca gccgaacaag tcggcggcaa aggaggcggc cgtcccgact tggcgcaggc
     7021 cggcggcacg gatgccggca aattgcccga aatgctggta agcgtggaaa gctggctttg
     7081 tcagaaatta agttaaattt aaatcttaat aagccatttg agtcttgttc aaatggctta
     7141 tattttggat tgttagaatg aatactaaaa cagagttaca aaaactttta gaagaggata
     7201 tatcaacttt aacagaaaca ctaatttgtg cagatgctct tcctccaaga tatgtacgta
     7261 gcattgcaac tcctattgtc cgtcgatggt taattgacaa acaattaaat attctcgcaa
     7321 aagaaatagg tttaactata gaattgccca tccttgatac aagtttggta tttgaaaaat
     7381 tgtcaacctt agagaataaa gttaattttt atatggctgg tggagtttat ttaggcggag
     7441 aatttattag ctctatatat cattcatcac aagaattttc tggtgaacca ataatctatg
     7501 ctgaaccaaa tataatttta tgtcctgctg aaaaattttt aactttaaaa cgagttttcc
     7561 ataatggaaa tatttttaat atgaatcaga taattacttt tttatccaat aaacaaggag
     7621 gagtacattt tgataaaaat tacgataaat ataaaacttg gcaagttgct atagaaaaag
     7681 cagcaaattt tttaaaactc ggtaatccat ataatgaaga taaattatct cttagtgaag
     7741 aacatgatac tattttagta gttttaccat tagaaaaagg atatgaatgg aattgtcttg
     7801 agatagaagt actatccgct gctcaatctc tagcaaatat ctattgcaac aaagttcgcc
     7861 tcatagatgg tcatgtttgg aaggaataaa agaaaaagca tagttggtaa tttttagtta
     7921 cgtagggtgg gcttcagccc accgtttttt catttttgat tgttgattgg tgggctaaag
     7981 cccaccctac ggttactaaa atcccgtcat tacctcgcag gcgggaatcc ggtttgttcg
     8041 gtttcggttt tttagggaca gtgccgcaac attaaatttc cagattccca ctttcgcaca
     8101 aataacagcg caaatagggc aaagataagg atatgggggg ttttttcttt gaatttacta
     8161 tacaattcgc ccgtccccgt tatagaaacc caagccgatg aaaaccctgc tccaactcca
     8221 aaccgccgca caaaacttcg ccgcctacta caaagaccaa accgacgaac gccgcgagaa
     8281 agacaccttc tggaacgaat ttttcgccat tttcggcatc gaccgcaaaa acgtcgccca
     8341 cttcgaatac cccgtcaaag accctgccga caacacccaa ttcgtcgata tattttggga
     8401 aggcatcttc cttgccgaac acaaatccgc caacaaaaac ctgaccaagg ccaaagagca
     8461 ggcggagcgt tatttgcagg aaatcgggcg caccaagccc tccgcgctgc ccgaatatta
     8521 cgccgtcagc gattttgccc atttccacct ttaccgccgc gtacctgaag aaggcgcaga
     8581 aaaccaatgg cagttccctt tggaagaact acccgaatac atcacgcgcg gcgttttcga
     8641 cttcatgttc ggcatcgaag ccaaagtccg ccaaattcaa gaagaagcca acattcaagc
     8701 ggcggcggcc atcggcaggc tgcacgacgc gctcaaagaa gaaggcattt acgaagaaca
     8761 cgagctgcgc ctcttcatca cgcgcctgct tttcctcttt tttgccgacg acagcgccgt
     8821 tttccagcgc aactaccttt tccaagactt tttagaaaac tgcaaagaag ccgacacgct
     8881 cggcgacaag ctcaaccaac tctttgaatt tctcaacaca cccgaccaaa agcgcagcaa
     8941 gacccaaagc gaaaaattta aaggtttcga atacgtcaac ggcggtctgt ttaaagaacg
     9001 cctgcgcact ttcgacttca ccgccaagca gcaccgcgcc ttaatcgact gcggcaattt
     9061 cgactggcgc a
//
LOCUS       DAWFHY010000081         9037 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.081, whole genome shotgun sequence.
ACCESSION   DAWFHY010000081 DAWFHY010000000
VERSION     DAWFHY010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.081"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            690..1904
                     /locus_tag="WGQ68_001700"
     CDS             690..1904
                     /locus_tag="WGQ68_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0715466.1"
                     /translation="MSEVEYFSHFISDGKGKLLEIPQRRGKQDGVFVDWISFTFHEDT
                     LLKVSGCPLFSDAEYMYVLSRKLEEILGFGITRKCKSRGNKFYESMYRLGSDDVDYGE
                     VHFGGQRNTVLVELKGTGCSVASPGWELRLKQFLDDSIRTRITRIDLALDFFDGEYTP
                     DQALLDHDNGFFDNSNQRPKSETIGTAWRNEDGSGKTFYVGRKKNSRFVRVYEKGRQL
                     GDKESKWVRFEIQFNYGDIEIPLDILINQGSYFCGAFPICRKFKNMPVPERFDQRKKT
                     LNLTFEHKLHYAKNAVGKLVNFMIEMGFDNSEIVESLKADSGFPKGLEPEKYALEMLR
                     DGLKHGFIHEQPDIDLEIELDELGVIAFKNSDKFDREKRLFSPDYDVEKERKYQEYLS
                     KVYHQNVDYDYF"
     gene            1916..2227
                     /locus_tag="WGQ68_001701"
     CDS             1916..2227
                     /locus_tag="WGQ68_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715467.1"
                     /translation="MFNQTQTVTYPATFLGAKKFKGEIDGSNIDTCSVLVATPLPAQS
                     GNAVGFTAAQMKFGDSKNFSRLENLKYPCEVMVMVEMTSTGKGMVPSLIDFQVAEKPK
                     G"
     gene            2231..2431
                     /locus_tag="WGQ68_001702"
     CDS             2231..2431
                     /locus_tag="WGQ68_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715468.1"
                     /translation="MKFEERFIVQDLETHDFIYPDPFGDVGFTQNIKSAGQFESYEDA
                     LNSGINEMGGGFQIFQFFVKSE"
     gene            2669..2899
                     /locus_tag="WGQ68_001703"
     CDS             2669..2899
                     /locus_tag="WGQ68_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715469.1"
                     /translation="MNIVKKYAVKALLAAGIFTPAIVMADGFDASAIGTQVANVIMGF
                     VAMVSAVGMAAITVILAIQGFKMAWSMIKSVK"
     gene            2927..3229
                     /locus_tag="WGQ68_001704"
     CDS             2927..3229
                     /locus_tag="WGQ68_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715470.1"
                     /translation="MGYRVGINCFDTRLQADDYLLSSLPPTVTQDGKIIRPERVGDKW
                     ILNGKPVTLSYPECSNFEQIKQGSYVGSTVLILFVVIYGFRLLINFLKDIGKVGTD"
     gene            3576..5207
                     /locus_tag="WGQ68_001705"
     CDS             3576..5207
                     /locus_tag="WGQ68_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T cell/B cell stimulating protein TspB"
                     /protein_id="HEZ0715471.1"
                     /translation="MFIILGRNFLKIILCFSFFVSKFALASVNAPGKFDRVEVYDDGR
                     YLGIRGSDDKRRRIWKGVFDRESGRYLTSEAQDLKVRHVSTGASSTGKVSSVVSSSVS
                     RAGVLAGVGKLARLGAKLSTRAVPYVGTALLAHDVYETFKEDIQARGYQYDTETDKFV
                     KGYEYSNCLWYEDERRINRTYGCYGVDSSIMRLMSDYSRFPEVKELMESQMYRLARPF
                     WNWHKEELNKLSSLDWNNFVLNRCTFNWNGGDCLVNKGDDFRNGADFSLIRNSKYKEE
                     MDAKKLEEILSLKVDANPDKYIKATGYPGYSEKVEVAPGTKVNMGPVTDRNGNPVQVV
                     ATFGRDSQGNTTVDVQVIPRPDLTPGSAEAPNAQPLPEVSPAENPANNPNPNENPGTR
                     PNPEPDPDLNPDANPDTDGQPGTRPDSPAVPGRTNGRDGKDGKDGKDGGLLCKFFPDI
                     LACDRLPEPNPAEDLNLPSETVNVEFQKSGIFQDSAQCPAPVTFTVTVLDSSRQFAFS
                     FENACTIAERLRYMLLALAWAVAAFFCIRTVSREV"
     gene            6152..6436
                     /locus_tag="WGQ68_001706"
     CDS             6152..6436
                     /locus_tag="WGQ68_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2523 domain-containing protein"
                     /protein_id="HEZ0715472.1"
                     /translation="MKLLAALIPLLMSVAGRILTALGLMAVTYSGVDRLVAHFQQAIT
                     NSITGAPQAMLQLFYISGGGTVLNILFGAIAFILSFKQMTKLATSIGKKK"
     gene            6437..7624
                     /locus_tag="WGQ68_001707"
     CDS             6437..7624
                     /locus_tag="WGQ68_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HEZ0715473.1"
                     /translation="MAEICLITGTPGSGKTLKMVSMMAGDEMFKPDENGIRRKVFTNI
                     KGLKIPHTYIETDAKKLPKSTDEQLSAHDMYEWIKKPENIGSIVIVDEAQDVWPARSA
                     GSKIPENVQWLNTHRHQGIDIFVLTQGPKLLDQNLRTLVRKHYHIASNKMGMRTLLEW
                     KICADDPVKMASSAFSSIYTLDKKVYDLYESAEVHTVNKVKRSKWFYTLPVIVLLIPV
                     FVGLSYKMLSSYGKKQEEPAAQESAATEQQAVLPDKTEGEPVNNGNLTADMFVPTLTE
                     KPESKPIYNGVRQVKTFEYIAGCVQGGRTGCTCYSHQGTALKEVTELMCKDYVKNGLP
                     FNPYKEESQGQEVRQSAQQHSDRAQVATLGGKPLQNLMYDNWQERGKPFEGIGGGVVG
                     SAN"
     gene            7839..8795
                     /locus_tag="WGQ68_001708"
     CDS             7839..8795
                     /locus_tag="WGQ68_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003694155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0715474.1"
                     /translation="MNIIGLDISKDTIDATLHKTNGSIHYIKFKNNDDGLKQFRLWIK
                     GNRIRKAYIGMEATGIYYEKAADMLSSYYTVYVINPLKIKDYGKSRFNRTKTDKADSN
                     LIADYIKRHQDTLIPYQIPKNKALQKLINLKNQLHQHQKQIKNRLHSTEEDFIRNIHQ
                     DLIDTIQDKMEQVKIAISEQIKKQTDNNHYRNLQTIPSIGKDTASVLYAQLTEKHFKT
                     ANQFVSYAGLSPAIIQSGTSVRGRGRLSRYGNRRLKSTLYMPALCAYRFNAFPKLINN
                     LKKAGKPKMVIIVAIMRKLAKLAYYIVKTGQPYDAERHRLNQ"
BASE COUNT         2756 a         1779 c         2167 g         2335 t
ORIGIN      
        1 cgtcattccc acgaaagtgg gaatccagaa tctcggactt tcagataatc tttgaatatt
       61 actgttgttc taaggtctag attcccgcct gcgcgggaat gacgggattt gaggttgcag
      121 catttatcgg gagcaacaga atccgctctg ccgtcattcc cacgaaagtg ggaatctagt
      181 tttttgagtt tcagtcattt ccgataaatt gccttagcat tgaatgtcta gattcccgcc
      241 ttatatgatg cgctctatca aaggggcgca ttaattttct taacattccc ctttgacagc
      301 caagtgaaag gggctttttt atgtcagcag taaatgtaat attttcctgt tcttattgga
      361 gaatatttaa aaaatcagat tcttgtgttt tgtgttttta tcagttcaga catggcgaac
      421 cgcataaact cattaatcaa gagaattttt caaagcttta tcaggcgttc gattatatag
      481 attcggttgg ttcgaatttt ccagtgatta tcacaacgga tggttgtggt cttttttgtt
      541 gatctttaaa agtttgtcag gatttggctt tcggtcgttg accgtcgtac gcgctttagc
      601 gcggaagacg ggaaacggct gaaagccccc ccccttgact aacagggggg ggagcgaaat
      661 taaaaaccaa ttccaagagt agtgaacgaa tgagtgaagt tgaatatttc tcacacttta
      721 tatcggacgg aaaagggaag cttttagaaa ttccgcagcg aagaggtaag caagacgggg
      781 tttttgttga ttggatttca ttcacattcc atgaagatac tttactgaaa gtttccggtt
      841 gccctttatt ttctgatgct gaatacatgt atgtattaag cagaaagctg gaagaaattc
      901 taggttttgg cataacgcgc aaatgcaaat caaggggcaa caaattctat gaatccatgt
      961 ataggttagg ttcggatgat gttgattatg gagaggtgca tttcggaggt cagcgcaata
     1021 ctgttttagt tgagttgaaa ggtactggtt gcagcgttgc aagtccgggt tgggagttga
     1081 ggctaaagca gtttctcgat gattcgataa ggacaagaat aacgcgaatt gacctagcac
     1141 ttgatttttt tgatggagag tacacgccgg atcaggcgtt gttagatcac gataatggtt
     1201 tttttgataa cagcaatcaa aggccgaaat ctgaaacgat cggtacggct tggcggaatg
     1261 aggacgggag cggcaagaca ttttatgtag gtcgcaagaa aaattctcgt tttgttcgtg
     1321 tttatgagaa aggcaggcag cttggagata aagaaagcaa atgggtaagg ttcgagatcc
     1381 agtttaatta tggagatata gaaataccct tggatatttt aataaatcag ggttcgtatt
     1441 tctgtggagc ttttccaatt tgtagaaaat ttaaaaatat gccggttccc gaaaggtttg
     1501 atcagagaaa gaaaacgctt aatttaactt tcgagcataa attgcattac gcgaaaaacg
     1561 cggttggaaa actggtcaat ttcatgattg aaatgggttt tgataatagc gaaattgtgg
     1621 aatctttaaa ggcagattcg ggatttccca aaggattaga acctgaaaaa tatgctctgg
     1681 aaatgttaag ggacggtttg aaacacggtt ttattcatga acagccggat attgatttgg
     1741 aaattgaact tgatgaattg ggggttattg cttttaaaaa ttctgacaaa ttcgataggg
     1801 aaaaaaggct ttttagtcct gattatgatg tcgagaaaga aaggaaatat caggaatatt
     1861 taagtaaagt ttatcatcaa aatgtagatt atgattattt ttaaaggaaa tcaaaatgtt
     1921 taatcaaact caaactgtaa cttatcctgc aacttttttg ggagccaaaa aattcaaagg
     1981 tgaaattgat ggctctaaca ttgacacttg ttccgtattg gttgcaacac ctttaccggc
     2041 acagtcgggt aatgctgttg gatttacggc agcacaaatg aagttcgggg atagtaagaa
     2101 tttctcaagg ttagagaatc tcaaatatcc gtgcgaagtt atggtaatgg ttgaaatgac
     2161 ttcgacaggt aaaggcatgg ttccgtcatt aattgatttt caggtggcgg aaaagccgaa
     2221 aggttgattt atgaaatttg aagaacgttt catagttcaa gatttagaaa cgcatgactt
     2281 tatttatccc gatccgttcg gtgatgtggg gtttactcaa aatattaaat cagcaggtca
     2341 atttgaaagt tacgaagatg cgttgaattc aggcataaat gaaatgggcg gaggattcca
     2401 gatatttcag ttcttcgtaa aatcggaata aaagaaaaac aggctcggcg ggcggtctgt
     2461 taacctttca caaagcccgc aacaaaggaa aaatatcatg aaaatgaacc ttgcaacact
     2521 aattatcggc tgggtggtct gtatgtttct ttttcttttc gcaatcctct attttatcgg
     2581 ctaaaaacga gattcggaaa agacttcgtc cggatgaagc aagtcaagaa gtcgtcttat
     2641 tttaaatatc aaaaaaagga aaaaaacgat gaacatcgtt aaaaaatatg ctgtaaaagc
     2701 attgttggca gctggtatct tcacaccggc tattgttatg gcagatggct ttgatgcatc
     2761 cgcgattggt acgcaagtgg cgaatgtaat catgggtttt gtggcaatgg tttccgccgt
     2821 gggtatggcg gccattacgg tcatcttggc aatccaaggc ttcaagatgg cttggagcat
     2881 gatcaaatct gtcaaataaa cagagtgaag aaaaaggggc gcataaatgg gctatcgtgt
     2941 cggcataaat tgttttgata caagattgca ggcagacgac tatttattgt cgtcccttcc
     3001 tcctactgtt acccaggacg gaaaaatcat caggccggaa agggtgggcg ataaatggat
     3061 tttgaacgga aagccggtta cgttgtctta tccggaatgt tccaattttg agcagataaa
     3121 gcaaggttct tatgtcggtt cgacggttct aattctgttt gtagtcattt acggtttcag
     3181 gcttctgatt aattttttaa aagacatagg caaggttggg actgattgat gattatagat
     3241 ttctggtttc ttctcggttt cttcttggct ttgtctgttg cttggctgtt ttggtaacgg
     3301 ttggtagaat cggcttttta gagtgtttta aaaggtccga attatgttta tttctgaata
     3361 tcatttagtt aaatttcaaa ctgattcaca tatttataga gatttaccac aagcgttaat
     3421 ttattatagg gaattgatta gaaaaggggt ttttaaaact tcgttttcat ttgatatttt
     3481 taggaatttc tttcatcgtt atgatagaga ttttatagaa attcaattcc ctgattcttc
     3541 tacattatta attaaattag atgaagcaaa atgttatgtt tattatccta gggcgaaatt
     3601 ttttaaagat tatcctatgc tttagttttt ttgtatctaa atttgcattg gcatcagtaa
     3661 atgctccggg taaatttgat agggttgaag tttatgatga tggcagatat ttaggtattc
     3721 gaggttcaga tgacaaaaga agaagaattt ggaaaggtgt atttgataga gaatcgggaa
     3781 gatatttaac ttcagaagct caagatttaa aagttaggca tgtatctact ggagcatcaa
     3841 gtacgggtaa agttagttcg gttgtatctt catcagtttc ccgcgctggc gtattggcgg
     3901 gggtcggcaa acttgcccgc ttaggcgcga aattaagcac aagggcggtt ccctatgtcg
     3961 gaacagccct tttagcccat gacgtatacg aaactttcaa agaagacata caggcacgag
     4021 gctaccaata cgacaccgaa accgacaaat ttgtaaaagg ctacgaatat agtaattgcc
     4081 tttggtacga agacgaaaga cgtattaata gaacctatgg ctgctacggc gttgacagtt
     4141 cgattatgcg ccttatgtcc gattacagca gattccccga agttaaagaa ttgatggaaa
     4201 gccaaatgta taggctggca cgtccgtttt ggaattggca taaagaagaa ctgaataaat
     4261 taagttcttt ggattggaat aattttgttt taaatcgttg cacatttaat tggaatggcg
     4321 gagattgttt ggtcaataaa ggtgatgatt tcagaaatgg ggctgatttt tcccttattc
     4381 gcaattcaaa atacaaagaa gaaatggatg ccaaaaagct ggaagagatt ttatcgttga
     4441 aagtcgatgc caatcccgac aaatacataa aggcaaccgg ttatcccggt tattccgaaa
     4501 aagtagaagt cgcacccgga acaaaagtga atatgggtcc cgtcacggac aggaacggga
     4561 atcccgttca ggttgtcgca acattcggca gggattcgca aggcaacacc acggtggatg
     4621 ttcaagtaat cccgcgtccc gacttgaccc ccggaagcgc ggaagcaccg aacgcacagc
     4681 cgctgcccga agtatcgccc gccgaaaacc ccgcaaacaa cccgaacccc aatgagaacc
     4741 ccggcacgcg tcccaatccc gaacccgacc ccgatttgaa tcccgatgca aatcccgata
     4801 cggacggaca gcccggcaca agacccgatt cccccgccgt tccgggccgc acaaacggca
     4861 gggacggcaa agacggaaag gacggcaaag atggcggcct tttgtgcaaa ttcttccccg
     4921 acattctcgc ttgcgacagg ctgcccgagc ccaatccggc agaagattta aatctgccgt
     4981 ctgaaaccgt caatgtagag tttcagaaat caggaatctt tcaagattcc gcacagtgtc
     5041 ccgcacctgt cactttcaca gtgactgtgc ttgattcaag caggcagttc gcgttcagct
     5101 ttgagaacgc atgtaccata gccgaacggc taaggtacat gcttctcgcc cttgcttggg
     5161 cggttgccgc ctttttttgt atccgcacag tatctcgtga agtctagcag gcgcagcacc
     5221 gccgggcttc agtaacttgt accaaggcag ggggaggacg tccagaaaga tttgtaaaga
     5281 cggctttatc gtctttataa atctttttgg ataccccttg ccgccccgcc aaaagaacac
     5341 attctgccgc aagggcaggt ggtaaggcgc gcgccttttg cgccgttccc cctgcccccg
     5401 cggcgtcgca agtgagactg ggggtgcggg ggctagtccc cgcaaagcct ttcagcttcg
     5461 gaagccacgg ccgaaaggca ggcgcagcac tgccggtctg agcggaagcc aggctacagg
     5521 caggcgcagc accgccgagc taggcggaag ccaggctaca ggcaggcgaa gcaccgccgg
     5581 ttgggcggaa gccacggccg aaaggcaggg cgaagcaccg ctaggcttag gcggaagcca
     5641 cggccgaaag gcaggcgaag taccgccggt ctgggcggaa gccatggtaa aaggcaggcg
     5701 aagcaccgcc gggcttcagt aacctttgtt caggcagggg gaggatgtcc gtaaagaatc
     5761 gtaaaggcgg gtttttttcg cctttatgat tctttatgga taccccttgc cgctcggaca
     5821 aagaaataca ctctgccgca agggcaggtg gtaaggcgcg cgccttttgc gccgtccccc
     5881 tgcccccgcg gcgtcgcaag tgagactagg gggtgtgggg gactagtccc ccgcaaagcg
     5941 ttcagcttcg gaaactttgg ccgaaaggca ggcgaagcag cgcactttgc gacgaatgtc
     6001 gcaaatagcc gagaagcgcg gggggattgg cgataagcgc gaggggggtg tccccacagc
     6061 gccgccgcgc cgcgaatgcg gcgcaaaatc tttcagatta agaaacattt gtttaatgag
     6121 gcaaccgtgc cttttaagaa agggatagca aatgaaattg ttggccgcat tgattccgct
     6181 tttgatgagc gtggcaggcc gtatattgac tgcattaggc ttgatggcgg taacctattc
     6241 aggggtggat agattggtag cccattttca gcaggcgata accaatagca taacgggcgc
     6301 gcctcaagcg atgttgcagc ttttttatat aagcggcggt ggaaccgttc ttaatatcct
     6361 gtttggtgcg atcgccttta ttctgtcatt taaacaaatg acaaaactag caacctcaat
     6421 cgggaagaaa aaataaatgg cagagatctg tttgataaca ggcacgcctg gttcagggaa
     6481 aacattaaaa atggtttcca tgatggcagg cgatgaaatg tttaagcctg atgaaaacgg
     6541 catacgccgt aaagtattta cgaacatcaa aggcttgaag ataccgcaca cctacataga
     6601 aacggacgcg aaaaagttgc cgaaatcgac agatgagcag ctttcggcgc atgatatgta
     6661 cgaatggata aagaagcccg aaaatatcgg gtctattgtc attgtagatg aagctcaaga
     6721 cgtatggccg gcacgctcgg caggttcaaa aatccctgaa aatgtccaat ggctgaatac
     6781 gcacagacat cagggtattg atatatttgt tttgactcaa ggccctaagc ttctagatca
     6841 aaatcttaga acgcttgtac ggaaacatta ccacatcgct tcaaacaaga tgggtatgcg
     6901 tacgctttta gaatggaaaa tatgcgcgga cgatcccgta aaaatggcat caagcgcatt
     6961 ctccagtatc tatacactgg ataaaaaagt ttatgacttg tacgaatcag cggaagttca
     7021 taccgtaaat aaggtcaagc ggtcaaagtg gttttacact ctgccagtaa tagtattgct
     7081 gattcccgtg tttgtcggcc tgtcctataa aatgttgagc agttacggaa aaaaacagga
     7141 agaacccgca gcacaagaat cggcggcaac agaacagcag gcagtacttc cggataaaac
     7201 agaaggcgag ccggtaaata acggcaacct taccgcagat atgtttgtcc cgacattgac
     7261 ggaaaaaccc gaaagcaagc cgatttataa cggtgtaagg caggtaaaaa cctttgaata
     7321 tatagcaggt tgtgtacaag gcggaagaac cggatgcacc tgctattcgc atcaagggac
     7381 agcattgaaa gaagtgacgg agttgatgtg caaggactat gtaaaaaacg gcttgccgtt
     7441 taacccatac aaagaagaaa gccaagggca ggaagttcgg caaagcgcac agcaacattc
     7501 ggacagggcg caagttgcca cgttgggcgg aaagccgctg caaaatctta tgtatgataa
     7561 ttggcaggag cgaggaaaac cgtttgaagg aatcggcggg ggcgtggtcg gatcggcaaa
     7621 ctgaagaaaa cggcaagaga gaaaaaagac ccataaaccg tttgaatata gacggcttac
     7681 gggtctttgt ttcgcgcaaa gcaaaggcta aggcagtcag gcagcaaatc ccgcaatgta
     7741 tcaaaacaga cgcgtagaaa tgccggctgc ctttatccat cctcaaaatt gaatatcatc
     7801 ctagccgtat caaggctgta taaataagga aaataccaat gaatataatc gggctggaca
     7861 tctcaaagga caccatagac gcaacattgc ataaaacaaa cggaagtatc cattacatta
     7921 aatttaagaa taatgatgat ggattaaaac agtttagatt gtggataaag ggaaacagaa
     7981 tcagaaaagc ctatatcggc atggaggcaa caggcatcta ttacgaaaag gcagcagata
     8041 tgctttcttc ctactatact gtttacgtta ttaatccctt aaaaatcaag gactacggaa
     8101 aaagcaggtt taaccgtacc aaaaccgaca aagcagattc aaacctgata gcagactaca
     8161 taaaaaggca tcaagataca ttgataccgt atcagatacc caaaaacaaa gcactgcaaa
     8221 aactgattaa ccttaaaaat caattacatc aacatcagaa gcaaattaaa aaccgtcttc
     8281 atagcactga agaagacttc ataaggaaca tacatcaaga cttgatagat accatacagg
     8341 acaagatgga acaggtaaaa atagccatat ccgaacaaat caaaaaacaa acggacaata
     8401 accattaccg caatcttcaa accatcccga gcataggcaa agacaccgca tcagttcttt
     8461 atgcgcaact gacagaaaaa cattttaaaa ccgcaaacca gtttgtatcc tatgccggat
     8521 taagtcccgc catcatacaa tcagggacaa gcgtaagagg tcggggcaga ttgagccgat
     8581 acggaaacag acgattaaaa agtacgctgt atatgcccgc cctttgtgct taccgtttta
     8641 acgcatttcc gaaattaata aataatctga aaaaagcggg taagccaaag atggtaatca
     8701 tcgttgccat catgcgcaaa ctggcgaagc tcgcctatta cattgttaaa accggccagc
     8761 cttacgatgc ggaaagacac cgattgaatc aataaaattc aacaaaatta aacggttacg
     8821 cgaatatatt tgtgtaaccg tgcatttgca tatcgtaaat aaacgtaaat aaaaataaca
     8881 atataaatca gtatattgca actttgtttt ttattttgtg ttgacgggca acatatcatc
     8941 tgcgcgggaa tgacgaatcc atccatacgg aaacctgcac cacgtcattc ccacgaaagt
     9001 gggaatctag gacgcaaaat ctcaagaaac cgtttta
//
LOCUS       DAWFHY010000082         8983 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.082, whole genome shotgun sequence.
ACCESSION   DAWFHY010000082 DAWFHY010000000
VERSION     DAWFHY010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8983
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.082"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(807..1169)
                     /locus_tag="WGQ68_001709"
     CDS             complement(807..1169)
                     /locus_tag="WGQ68_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0715475.1"
                     /translation="MKAARRQLYYTFANRIAEQSRIICIKKLNLLNLQCIQIEVNKLH
                     QKIVASSLLLHMLKEGAAKNGVHIEEIKANATSQSCYSCGRLNKRIGVAATRTNMVCE
                     GFGRHYDTDENASSNIRK"
     gene            1589..2953
                     /locus_tag="WGQ68_001710"
     CDS             1589..2953
                     /locus_tag="WGQ68_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0715476.1"
                     /translation="MQHLTERMTQIAKLLNSSPNNPDIDIPDFLTEIKDYSEFSVTDE
                     NGTYLHWDKFRRIHTEDTRMKWRAVKESRKKIQKPIDFPFEHQFWFCIPDSLQARLHL
                     IDKSCSSSIGTSSLGGFGRSEQNRFLLKSLIMEEAITSAQLEGAATTRKVAKDMLKSQ
                     RKPKTKDEIMIVNNYHLMKKAVELKNTPLSVEMILDLHRIATSNAIENKAEPGQFRQY
                     DEIFIADINGNSLYQPPPHGQVHTLMEEVCAFANNTYDGVENPFIHPVVQAIILHFLI
                     GYIHPFGDGNGRTARALFYWFMLKNGYWLFEYISISRLLKNAPAQYAKSYLYAETDDL
                     DLTYFIYYQCDIIKRAVADLEHYISDKQKHQQEFKAAIAQYTEKIGKLNQRQIGILQK
                     AVEESGKIFTAQEIANQYGISLNTARSDLSKLGEYRFLVPFKSGNALEYVAPQDLLER
                     LEKK"
     gene            3355..3819
                     /locus_tag="WGQ68_001711"
     CDS             3355..3819
                     /locus_tag="WGQ68_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715477.1"
                     /translation="MLHIPPTEPDRYVSSIVALNVHSSNGTGDWHSAAALNDDAYPQD
                     FYIYGKDQTRSTLHLLGNVGVIDGTNRFNEMGYYPENIPVWLADHPRACVDYLYTAVL
                     QTGSIGRVILDDWFPSDEDKQSVYDLLNQIEPHLNTQEWGNLQLWKRKNPIM"
     gene            3946..5235
                     /locus_tag="WGQ68_001712"
     CDS             3946..5235
                     /locus_tag="WGQ68_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HipA family
                     toxin"
                     /protein_id="HEZ0715478.1"
                     /translation="MMKSILTVSGNRMRKPRITYLDVWANDERIGTLEKGAMYRFAYD
                     NPNSSLLGLHYQDRSKVYISNNMPHIFAQYFPEGFLDAHITSKYAFHDAPFEDNEMLR
                     LAILGRETLGRIHVRCNDPLFNEWIDGLEMKNPRILTERDLLGINARQVFQQYMAEIF
                     HHGRFVSVSGIQQKMSLDAISRNTKQTASYIAKGFDASEYPCLAANEFLCMQTIKQAG
                     IAVAQTSLSEDSSVLLVRRFDVSEQGYFLGMEDFTSLCQYSAEDKYKGSYAAIAQIIR
                     QISGRPDEDLIHFFNQLAASCILKNGDAHLKNFSVLYHDEYDVRLAPVYDVLDTSIYR
                     VGTQGIFDAYDDTLALNLTNHGKKTYPSKNTLLDFAEKYCDLGREDASFMIDTIVQAK
                     EQVLVKYSDVLRENEWLAQKWHFIPDENEEGLPFTFR"
     gene            complement(5560..7347)
                     /locus_tag="WGQ68_001713"
     CDS             complement(5560..7347)
                     /locus_tag="WGQ68_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HEZ0715479.1"
                     /translation="MKLPLSYLPNIRFLSWCCLLAGIIAPATLLASPNPAEIRMQQDI
                     QQRQREEQLRQTMQPESDVRLHQKNTGETVNQLMGDDSSQPCFAINEVVLEGEHHARF
                     QFALKRALRETGFQAGKCLHAGNINQIMSLAQNALIGRGYTTTRILAAPQDLNSGKLQ
                     LTLIPGYLRSIRIDRSNDDQTHAGRIAAFQNKFPTRSNDLLNLRDLEQGLENLKRLPT
                     AEADLQIVPVEGEPNQSDVVVQWRQRLLPYRVSVGMDNSGSEATGKYQGNITFSADNP
                     LGLSDMFYVNYGRSIGGTPDEESFDGHRKEGGSNNYAVHYSAPFGKWTWAFNHNGYRY
                     HQAVSGLSEVYDYNGKSYNTDFGFNRLLYRDAKRKTYLGVKLWMRETKSYIDDAELTV
                     QRRKTAGWLAELSHKEYIGRSTADFKLKYKRGTGMKDALRAPEEAFGEGTSRMKIWTA
                     SADVNTPFQIGKQLFAYDTSVHAQWNKTPLTSQDKLAIGGHHTVRGFDGEMSLSAERG
                     WYWRNDLSWQFKPGHQLYLGADVGHVSGQSAKWLSGQTLVGTAIGIRGQIKLGGNLHY
                     DIFTGRALKKPEFFQSRKWASGFQVGYTF"
     gene            complement(7402..7860)
                     /locus_tag="WGQ68_001714"
     CDS             complement(7402..7860)
                     /locus_tag="WGQ68_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-activating lysine-acyltransferase"
                     /protein_id="HEZ0715480.1"
                     /translation="MPITPPLNIISPKLYPNEQWNESEALGAITWLWYQSPTHRQVPI
                     VEMMTYILPVLKNGQFALFCKGTQPIGYISWAYFDEVAQAHYLESDRHLRDNSDWNCG
                     DNIWLIQWFAPFGHSHQMRSAVRQLFPSTTVRALYHKGSDKGLRILTFKT"
     gene            complement(8026..8379)
                     /locus_tag="WGQ68_001715"
     CDS             complement(8026..8379)
                     /locus_tag="WGQ68_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003680143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715481.1"
                     /translation="MEKNMKQILFKYFMLITCVFGLSSCGYLLSFGQLMFDGSCADWN
                     CSKPTQRWAKAVDTCETENLTIVLQVLGEDLGYKSLVEVQVSEIGQHISGQCTYYDGR
                     YDVRKDKQYGHLFVQ"
     gene            complement(8443..8760)
                     /locus_tag="WGQ68_001716"
     CDS             complement(8443..8760)
                     /locus_tag="WGQ68_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715482.1"
                     /translation="MKKTRSNMVLILFCSTILTGCFGSCGDWNCSGNPRWNEKVNDCY
                     SRNLEVVKQQLGYKKYGGDIVRFHSEVQDSEIGQHISTQCIYYSGKYDIHKDKQYGHL
                     FVK"
     gene            complement(8741..>8983)
                     /locus_tag="WGQ68_001717"
     CDS             complement(8741..>8983)
                     /locus_tag="WGQ68_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715483.1"
                     /translation="FCYSHSSYFAEVPEQYQRDRNGEYILDKNNNKIETEDWKDYKGK
                     WGVPKMGDDGKYINHAIPKLVYPNKLNGEQYEENPF"
BASE COUNT         2611 a         1873 c         1853 g         2646 t
ORIGIN      
        1 ggtaggggac cgatacgctg tttcatctct ttgcttaggg agagtagggg gatatgacct
       61 tagttttagt atccgtaata tcatcatttt tttcgtcaag ggaatatatc gactccagaa
      121 gacaggtatt agatactgcc ttttcttaca agagtgatgg taggatggtt ctcttcaagt
      181 caatcaaaca ggaaagtatt tcttttctgt ctgaagattt gaaaaaggac tggatgtttc
      241 acaaggttaa aactggggaa aaagatggat atggttcaga tgaaatgctg agcgtacccc
      301 gtgtctattt ggaaatgatg tcgcggaaaa cgggagtccc ctactccagt attctttaaa
      361 ttctaagcag aagacttctt cgtcggtctt tttttgttgt ttggtttgca tggagtaaaa
      421 ctgtacaact gttgaagcat aaaaccagca agaaggtaaa aaagaaagaa gcagtttttt
      481 tggattttag atgttaccgc aattagtttc ctttcctaaa atttgtttaa attatttgca
      541 atattaatat aaacgagata ttaatgatga gaaatcaaaa aggcataatg aatatatttt
      601 gtacaaaata tttgcagtat ttaaaaatgt tgatttgtat atgaaaagtt aaagatgcca
      661 aaatgtacag ttgctaaact gtaaaactgc taaagcaaca aaacataaaa aggaatgcag
      721 ggatgcaatc actacatctt tttattccgt aagcatttat gactttacgg tcaactgcta
      781 ctctatgttt ccagcttttc agctccctat tttcgaatat tggacgaggc attttcatca
      841 gtgtcgtaat gccggccgaa accctcacaa accatattgg ttcttgtggc agcaacacct
      901 atccgtttgt tcaagcgacc acaagagtaa catgattggc tggtagcatt ggctttaatc
      961 tcttcgatat gaactccatt tttagctgca ccttctttca gcatatgcag cagtagggat
     1021 gaggcaacta tcttctggtg gagtttgttg acttcaatct gtatgcactg taggttgagc
     1081 agattcaatt ttttgataca gattatccgg ctttgttcgg caattctgtt ggcgaacgta
     1141 tagtagagct gtcgtcttgc agctttcaat ttgcggtagg tattttacca ttcaatgcgt
     1201 agccgctcgg tacgtttgag ccaaatgtta tcttcgccat ttgtaccaat tggtttttac
     1261 attcggctgt gttttagtaa tttattaatt tcaattattt gcaagggaaa agacaattgt
     1321 ttccggttag gaataaacct atcctattga atatattgaa gccaagtacg tctatcaaca
     1381 ctatattaaa acacagcctt ttttaatata gtagacacaa tctttcccta tttatgaagg
     1441 tgatcgcttc tttcagattc gtattttaat actttctatt tctagaaaat ttgactaaaa
     1501 taactcaatt ataaaaaatt gcgcgatttt ggtatttatc atgaaaattt ccagacctcc
     1561 ggaatttacc ctgttgcaac aggaatatat gcagcatctc actgaaagaa tgacgcaaat
     1621 tgccaagctg ctgaattctt ccccaaacaa tcctgatata gacattcccg attttcttac
     1681 tgaaatcaaa gattattcag aattttccgt gacagatgaa aatggaacct acctgcattg
     1741 ggacaaattc cgccggattc acacggaaga tacgcggatg aaatggcgcg ccgttaagga
     1801 aagccgcaaa aaaatccaaa aaccaattga tttcccgttt gaacatcagt tttggttctg
     1861 cattcccgac tctttgcagg cacggcttca tttgattgac aaaagctgca gcagttctat
     1921 cggcacgtct agcttgggtg gtttcggcag aagcgagcaa aacagattct tgctcaagtc
     1981 tctgattatg gaagaagcga ttacatccgc ccaactggaa ggtgcggcta ccacgcgtaa
     2041 agtggccaag gatatgctca aatcgcagcg taaacccaaa acaaaagacg aaatcatgat
     2101 agtgaacaac tatcacttga tgaaaaaagc ggtagaattg aaaaatacgc cgttaagtgt
     2161 tgaaatgatt ttggatttgc accgcattgc taccagcaac gctattgaaa acaaggccga
     2221 gcccggacaa ttcaggcagt atgacgaaat ctttatcgcc gatatcaatg gtaacagcct
     2281 gtatcaacca ccgccgcacg gacaggttca tacgctgatg gaagaggtgt gtgcgtttgc
     2341 caataatacc tatgacggcg tggaaaatcc gtttatccat ccggttgtcc aagctattat
     2401 cctgcatttc ctcatcggct acatccaccc atttggtgat ggcaacgggc ggacagcgcg
     2461 ggctttgttc tattggttta tgctcaaaaa cggctactgg ctatttgaat acatatccat
     2521 cagccgtctt ctgaaaaacg ctcctgccca atacgccaaa tcctatttgt atgcggaaac
     2581 tgacgattta gatttaacct atttcatcta ttaccaatgc gatattatca agcgggcggt
     2641 tgctgatttg gagcactaca tttctgacaa acaaaaacac caacaggaat tcaaagcagc
     2701 gattgcccaa tatactgaaa agataggaaa gttgaaccaa cggcaaattg gtatcctgca
     2761 aaaagcagtg gaagaaagcg gaaaaatctt tactgcacaa gaaattgcca accaatacgg
     2821 catctccctg aatactgccc gtagcgattt gagcaaactg ggagaatata gattcctagt
     2881 gccgttcaaa tcaggaaatg ctttagagta tgttgctcct caggatttat tggaaaggtt
     2941 agaaaaaaaa tagtttgcta gcctaaaatg cagctttaac cgagtcaaaa tcaatacagt
     3001 ccgcaccttc aaaaagaagc tgcggactgc ttgctttttg ctctacaaat gatctttgta
     3061 gctgatttaa ccaagattgt agcaattttg ctttccgagc aagcagggtt agaaaattcg
     3121 atacttttaa ttattggctg tgttttaata tgatgttgat aggcgtgttt ggctttaagg
     3181 tattcaacag gataggttta ttcctaaccg gaaaataatt gtcttttccc ttgcaagtaa
     3241 ttgaaatcaa cagattacta aaacacagcc ttacattatt ggggtgacta tcctgtaaaa
     3301 tatgtcctaa aacgtggaaa ccacttttgc tctgctaaat tttaaggaat ctttatgtta
     3361 catatacccc caacggaacc tgatcgatat gttagcagta ttgtagcctt aaacgtgcat
     3421 agttctaacg gtacaggcga ttggcacagt gcagcagccc ttaatgatga tgcttacccc
     3481 caagatttct atatttatgg caaagatcaa acacgtagca cgttacattt gcttggtaac
     3541 gttggtgtga ttgatggaac aaaccgcttc aatgagatgg gctattatcc tgaaaacatc
     3601 ccagtttggc tcgcagatca cccccgtgct tgcgtggatt atctttacac agcagtgtta
     3661 caaactggct caatcggtcg ggtgatttta gatgattggt ttccaagcga tgaagacaag
     3721 caatcagttt atgacttact taaccaaatc gaaccgcact tgaatactca agaatggggg
     3781 aatttacagc tatggaaacg caaaaaccca ataatgtaat gctaaccgaa cgagataaaa
     3841 ggcacgagaa gcggtattgc tgaatttcat ccgcaatacg ccatatacaa ttaaatcagc
     3901 agatgtgtct aaacgtcccg ttgttcccgt ttgaaagaat ttttcatgat gaagtctatc
     3961 ctcaccgtat ccggaaatcg tatgcgtaaa cccagaatca cctatttgga tgtttgggca
     4021 aacgatgaaa gaatcggtac tttggaaaag ggagccatgt atcggttcgc atacgacaat
     4081 cccaattctt cgttgctggg cctgcattat caagacagaa gcaaggtata tatcagcaac
     4141 aacatgccgc atatctttgc acagtatttt ccggaaggct ttttggatgc acacatcaca
     4201 agcaaatatg cttttcatga tgcgcctttt gaagacaatg agatgctgcg cttggcaatt
     4261 ctaggcagag agactttggg tcggatacat gtgcgctgta atgacccgct ttttaatgaa
     4321 tggatcgacg ggttggagat gaaaaatcca agaatattga ctgaacggga tttgctgggc
     4381 ataaatgccc gacaggtttt tcagcaatat atggcagaaa tcttccatca cggccgtttc
     4441 gtcagtgtat ccgggataca gcagaagatg tccttagatg ccatcagcag aaataccaag
     4501 caaactgcct catatattgc caaaggtttt gatgcatccg aatatccttg cttggctgcc
     4561 aatgaatttt tatgcatgca aaccatcaaa caagccggca ttgccgttgc acagaccagc
     4621 ctgtcggaag attcatcagt cttattggta cgtcggtttg atgtcagtga acaggggtat
     4681 ttcttaggca tggaagactt taccagtctg tgtcagtatt cagcagaaga taagtataaa
     4741 ggcagttatg cggctattgc acagattatc cgacagatat ccggcagacc agatgaagat
     4801 ttaatccatt tctttaatca gcttgctgcc agttgcatat tgaaaaacgg cgatgcacac
     4861 ctcaaaaatt tttcagtact ctatcatgac gaatacgatg ttcgtcttgc acctgtctat
     4921 gatgtattgg atacatcaat atacagggtt ggaacacaag gaatttttga tgcttatgac
     4981 gatacgctgg cattaaacct gactaaccac ggtaagaaaa catatccttc caagaataca
     5041 ttgttggatt ttgctgagaa atattgcgat ttgggaagag aagatgcatc ctttatgata
     5101 gatacaatcg ttcaagctaa agaacaggtt cttgttaaat actcggatgt attgcgtgag
     5161 aatgaatggt tggcgcagaa gtggcatttt atcccggatg aaaatgaaga aggtctaccg
     5221 tttacattcc ggtagctgcc gctgtcagag atggccggtc gactttcacc ctgaaaatca
     5281 cttcatctta tggtgtttga aaccgagaaa ttagaagaat cgtattcggt aggagatata
     5341 ctgggaagat tggaaaactg gtaaatcact ctattgattg agttggcggc ctatatgttt
     5401 aatgtaggca aacgagaagg aatcatgtat ttcatgattc cttcttaaat tcctgtgtca
     5461 atctaatatc aaaacacagc catctcttac cataatcatg ataggtgttt tattatgaaa
     5521 agctatatct atagttaccg ttgatttgac tatgccgttt taaaacgtat agcctacctg
     5581 aaaaccgctt gcccatttcc ttgattggaa aaattcgggc tttttcaatg cgcggccggt
     5641 aaatatatcg taatgcaggt tgccgccaag ctttatctgc ccgcgtatcc caattgctgt
     5701 gccgactaga gtttggcccg ataaccattt ggcggattgt cctgaaacat gtcctacatc
     5761 agccccaaga taaagctgat ggcctggttt aaattgccag ctcaaatcgt tgcgccaata
     5821 ccatccccgc tcggcagaca aactcatttc accgtcgaag ccacgtacgg tgtggtgtcc
     5881 gccgatagcc agtttgtctt gcgatgttag cggggttttg ttccattgtg catgaacgga
     5941 tgtgtcatag gcaaatagct gtttaccgat ttgaaaagga gtatttacat cagccgatgc
     6001 cgtccaaatt ttcatacgtg acgtgccttc gccaaaggct tcttcaggcg cgcgcagagc
     6061 atctttcatg ccggtgccgc gtttatattt caacttaaaa tctgccgtac tgcgaccgat
     6121 atattctttg tgggaaagtt ctgccaacca acccgcagtt ttacgccgtt gtacagtcag
     6181 ttcggcatca tcaatgtaac tttttgtttc cctcatccac agttttacac cgagataggt
     6241 tttgcgtttg gcatcacgat acaacaggcg gttgaagccg aaatcagtat tgtaactttt
     6301 tccattatag tcatagactt ccgataatcc ggaaactgcc tgatggtaac ggtagccatt
     6361 gtgattgaat gcccatgtcc atttaccgaa aggggctgaa taatgtacgg cgtaattgtt
     6421 tgatccgcct tctttgcgat ggccgtcaaa actttcctca tcgggcgtac cgccaattga
     6481 acgtccataa tttacataga acatatcact cagtcccaaa ggattgtcgg cagagaaagt
     6541 gatatttcct tggtattttc ctgtcgcctc actacccgaa ttatccatcc ccacactcac
     6601 acggtagggc agcagacgtt gccgccattg caccacgaca tcactttggt ttggttctcc
     6661 ctctacggga acgatttgga gatcggcttc cgcagtcggg agacgtttga gattttccag
     6721 tccttgttcc aaatcacgca gattcaacag atcgttcgag cgggtgggaa atttgttctg
     6781 gaatgctgca atacgtcctg catgggtttg atcatcgtta gaccgatcga ttcgtatgga
     6841 gcgcagatag cccggtatca gggttaattg aagcttgcca ctattcaaat cctgtggcgc
     6901 agccaagata cgggtcgtgg tatatcccct gccgatcaaa gcattttgtg ctaaggacat
     6961 gatttgatta atgttgcccg catgcagaca cttgccagcc tgaaaacccg tttcgcgcaa
     7021 ggcacgtttt agggcaaact gaaaccgagc atggtgttcg ccttccaaca ccacttcgtt
     7081 aatggcaaaa cacggttggc tgctgtcatc gcccatcaac tgattaaccg tttctcccgt
     7141 gtttttttga tgcaaacgca catcgctttc aggctgcatg gtttggcgca actgctcttc
     7201 gcgttggcgt tgctgaatat cttgctgcat acggatttcg gcagggttgg gggaggccaa
     7261 caaagtagca ggagcaatga tacctgccaa taagcagcac caagacaaaa agcgaatatt
     7321 aggcaaatag gataaaggaa gtttcatggc atggtcgcaa aatcaataaa atcatcaatg
     7381 ggcattcaat acacgattgc atcaagtttt aaaagttaaa attctcaaac ccttatcgct
     7441 ccctttatga tacaaggcgc gtactgtcgt actaggaaat aactggcgca cagctgagcg
     7501 catttgatga ctgtgtccaa atggcgcaaa ccattgaatc agccaaatat tgtcgccaca
     7561 gttccaatcg ctgttgtcac gcaaatggcg gtcagattct aaataatgcg cctgcgccac
     7621 ttcatcaaaa taagcccatg agatataacc gattggttgg gtacccttgc aaaacaaagc
     7681 gaactgcccg ttttttaaca caggcaatat atacgtcatc atctccacaa taggtacttg
     7741 gcgatgcgta ggcgactgat accatagcca agtgatggca ccgagtgctt cgctttcgtt
     7801 ccattgttca ttggggtaga gtttagggga gatgatgttt aagggtggag tgatgggcat
     7861 attttaagtt agggttcgtg ttggttaaaa caaaaaatgg tttcagcctg aaatgaaata
     7921 tttcacgtta aaacatagtt ttagcacatg ggactgaact gcgtgggcaa cgagttgccc
     7981 accctaccat gtaacagttc gtattaattg cacaggctac gctggttatt gcacaaacaa
     8041 atgaccatac tgcttatctt tgcgaacatc gtatctgcca tcatagtaag tacattgtcc
     8101 agaaatatgt tgcccaattt cactgacttg tacctcaacg agagatttgt atcctaaatc
     8161 ttcccctaat acttgtaaca ctattgtgag attttcagtt tcacatgtgt caacggcttt
     8221 tgcccatctt tgagtaggct tggagcaatt ccaatctgcg caactgccat caaacattaa
     8281 ctgcccaaat gataataaat aaccacaact gcttaatcca aaaacacaag taattagcat
     8341 aaaatattta aaaaggattt gtttcatatt tttctccatt cggattattg ggattgacta
     8401 attttggaat agcgtgattt atgtatttgc tatcattact agctatttca caaacaaatg
     8461 accatactgc ttatctttat gaatatcata tttaccgcta tagtaaatac attgcgtaga
     8521 gatatgttgt ccaatttcgc tatcttgcac ttcgctatgg aaacgaacaa tatcaccacc
     8581 gtatttttta tatcctaatt gttgcttaac aacttcaagg tttctactat aacaatcatt
     8641 aactttttcg ttccatcgcg gattacctga acaattccag tccccgcaac taccaaaaca
     8701 gccagttaat attgttgagc aaaacagtat caataccata ttagaacggg ttttcttcat
     8761 attgctcacc attgagttta ttcggataca ctaatttcgg aatagcatga ttgatatatt
     8821 tgccgtcatc acccatttta ggaacacccc atttcccctt atagtctttc caatcttctg
     8881 tttctatttt gttgttgttc ttatcaagga tatattcacc attcctatcc ctttgatact
     8941 gctcaggcac ttctgcaaaa taactgctat gcgaatagca aaa
//
LOCUS       DAWFHY010000083         8792 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.083, whole genome shotgun sequence.
ACCESSION   DAWFHY010000083 DAWFHY010000000
VERSION     DAWFHY010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8792
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.083"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(265..2955)
                     /gene="glnE"
                     /locus_tag="WGQ68_001718"
     CDS             complement(265..2955)
                     /gene="glnE"
                     /locus_tag="WGQ68_001718"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HEZ0715484.1"
                     /translation="MSDNRLDAARRHSLFLARNLDSGKLKPEIFLPMLDKVLTEADFQ
                     AFADWDAIRAEENEEELARQLRGLRRYVVSQIIVRDINRISDLNEVTRTITLFADFAV
                     NTALDFAYAYYRDMYGTPIGRYTKSPQHLSVVAMGKAGGYELNVSSDIDLIFVYPESG
                     DTDGRRERGNQEFFTKVGQKLIALLNDITADGQVFRVDMRLRPDGDSGALVLSETALE
                     QYLITQGREWERYAWCKGRVVTPYPNGIKSLVRPFVFRKYLDYGAYEAMRKLHRQIRS
                     EVGKKGMADNIKLGAGGIREVEFIAQIFQMIRGGQMRALQLKGTQETLEKLAETGIMP
                     SENVETLLAAYRFLRDVEHRLQYWDDRQTQTLPASPEQQQLLAESMGFDSYTAFSDGL
                     NVHRNKVNQLFNEILSEPEEQTQSNSEWQWAWQEKPDEEERLGRLKEYGFDAKTIATR
                     LDQIRNGHKYRHLSAHAQPRFDAIVPLFVQAATEQPNPNDTLMRLFDFLENISRRSAY
                     LAFLNEHPQTLAQLAEIMSQSSWVAAYLNKYPILLDELISAQLLDTAFDWDKLAAALS
                     DGIEACGGDTEAQMDTLRRFQHAQVFRLAVQDLAGLWTVESLSDQLSALADTVIAAAL
                     SCAWADMPKKHRDTPQFAVVGYGKLGGKELGYASDLDLVYLYDDPHPEAGDVYSRLAR
                     RLTNWLSAATGAGSLYETDLRLRPNGDAGFLSHSIAAFEKYQRENAWTWEHQSLTRAR
                     FICGTPEIQTAFDRIRTEILTAERDQTALAGEIIEMREKMFPTHPPADSNVKYARGGV
                     VDVEFIVQYLILAHARKYPQLLDNYGNIALLNIAADCGLIDKTLAGQSRTAYRFYRRQ
                     QHNTKLRDAAKTEVSDELPVHYGNVRKLWKEVFGEEAATA"
     gene            complement(3350..3541)
                     /locus_tag="WGQ68_001719"
     CDS             complement(3350..3541)
                     /locus_tag="WGQ68_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715485.1"
                     /translation="MGFWNGVAKAVGEGMIEAANEHKALKMEYAEKSSEELHEIVKSD
                     GFLKIPHGRKVRLETFAIT"
     gene            complement(3678..4151)
                     /locus_tag="WGQ68_001720"
     CDS             complement(3678..4151)
                     /locus_tag="WGQ68_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2247 family protein"
                     /protein_id="HEZ0715486.1"
                     /translation="MNLDLTAQKVRLSWKDILWGYGNKYLGWADVAAYARKMTLSDHD
                     ERVFKLSLINKSNILELKPVLEDLASEMRDYSPKNWLYVLLSDVFHRKEEFDDPLGEV
                     EKIYADFDYPEEIESFVRYMPPKDGYIPSAHTYEENIARLYSHWEHYLNNGGGQG"
     gene            complement(4288..5313)
                     /locus_tag="WGQ68_001721"
     CDS             complement(4288..5313)
                     /locus_tag="WGQ68_001721"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEZ0715487.1"
                     /translation="MYPLARRILFALDAEKAHHFTLDALYTVYKLGLIPVTGNRTKPV
                     KLMGMDLPNPVGLAAGLDKNGEYIDALGALGFGFIEIGTVTPKPQPGNPQPRLFRVPE
                     YQGIINRMGFNNHGIDTMIRNIEKSKFSGVLGINIGKNAVTPIENAADDYLICLEKAY
                     AHASYITVNISSPNTKNLRALQGGDELSALLEALKNKQAQLASVHGKYVPLAVKIAPD
                     LDEAQIEDIAHVVKSVEMDGIIATNTTIDKSSLGSHPLAGEQGGLSGLPVHEKSNRVL
                     KLLADHIDGKLPIIGVGGIMEGRDAAEKIRLGATAVQVYSGLIYKGPALVKECLKALA
                     RSAQNAV"
     gene            5545..5781
                     /gene="acpP"
                     /locus_tag="WGQ68_001722"
     CDS             5545..5781
                     /gene="acpP"
                     /locus_tag="WGQ68_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEZ0715488.1"
                     /translation="MSNIEQQVKKIVAEQLGVNEADVKNESSFQDDLGADSLDTVELV
                     MALEEAFGCEIPDEDAEKITTVQLAIDYINAHNG"
     gene            5937..7184
                     /gene="fabF"
                     /locus_tag="WGQ68_001723"
     CDS             5937..7184
                     /gene="fabF"
                     /locus_tag="WGQ68_001723"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEZ0715489.1"
                     /translation="MSQRRVVITGLGQVSPVGNTVAEAWDTLLAGKSGIGAITRFDAS
                     DINSRVAGEVRGFDIGQYISAKEARRMDVFIHYGIAAALQAIADSGLDDVENLDKDRI
                     GVNIGSGIGGLPSIEVTGKAVIEGGARKINPFFIPGSLINLISGHVTILKGYRGPSYG
                     MVSACTTGAHAIGNSARLIKYGDADIMVAGGAEGAISTLGVGGFAAMKALSTRNDDPA
                     TASRPWDKGRDGFVIGEGAGILVLEELEHAKKRGAKIYAEIVGFGMSSDAYHITAPNE
                     EGPALAVTRALKDAGINPEDVDYVNAHGTSTPLGDANETKALKRAFGEHAYKTVVSST
                     KSMTGHLLGAAGGVEAVYSILAIHDGKIPPTINIFEQDVEAGCDLDYCANEARDAEID
                     VAISNSFGFGGTNGTLVFKRFKG"
     gene            7489..8622
                     /locus_tag="WGQ68_001724"
     CDS             7489..8622
                     /locus_tag="WGQ68_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEZ0715490.1"
                     /translation="MKIVFITTVASSIYGFRAPVIKKLIGKNHQVYAFVSEFSDNELD
                     IIREMGVTPVTYRSNRSGLNPFSDIKSTFLIFKELKKISPDLVFPYFAKPVIFGTFAA
                     KLAGVPRIVGMLEGLGFAFTPQPEGIPLKTKIIKGILIALYHIALPMLESLIVLNPDD
                     KDELTDKYGIKIKNIHILGGIGLDLRQYPYSEADIPDEKEPVKFLFIGRFLKEKGIDD
                     FIRAAEQVKDKYPDTVFTALGAIDKSRGGWGGDLERLAARDIIRFPGFVNNVSEVIKE
                     HHIFVLPSYYREGVPRSTQEAMAVGRAVITTDVPGCRETVADKVNGFLIEPWNPRILA
                     EKMIYFIENREAVRLMGNASYAIAKDKFDAEKVDLKLLDILKA"
BASE COUNT         2092 a         2284 c         2295 g         2121 t
ORIGIN      
        1 ccgtctaaaa agtagaatgc gctattttaa tggaaacggc ggggttttaa acggttctta
       61 tttgtttgtt ttgatttttg cttgggtaac aaatcccatc attcccacaa aaaaaatccc
      121 gtcattcccg cgaaagagga atccggtttt tgggtttcag ccattcccga taaatcgctt
      181 tagctctgcc gcaaccttaa aacggcacgg tacgaaaatg ccgtctgaaa cccagattgt
      241 caggcttcag acggcatttt ttgttcaggc ggttgccgct tcctctccga acacttcctt
      301 ccacaatttc ctgacattgc cgtaatgtac tggcaattcg tcgcttactt cggtttttgc
      361 cgcgtcgcgc agtttggtgt tgtgctgctg ccggcggtag aagcggtagg cggtgcggct
      421 ttgtccagca agcgttttat caatcaaacc gcagtcggcg gcgatgttca agagggcgat
      481 gttgccgtag ttgtcaagga gttgcggata ttttcgggcg tgggcaagta tcagatattg
      541 gacgataaat tcgacatcga ccacgccgcc gcgcgcgtat ttgacgttgc tgtcggcagg
      601 cggatgggtg gggaacattt tttcgcgcat ttcgataatt tcgcctgcca aggcggtttg
      661 gtcgcgttcg gcggtgagga tttcggtgcg gatgcggtcg aaggccgtct gaatttcggg
      721 tgtgccgcag ataaaacggg cgcgggtgag ggactggtgt tcccacgtcc aggcgttttc
      781 gcgctggtat ttttcaaagg cggcgatgct gtgggagagg aaacctgcgt cgccgttggg
      841 gcgcaggcgc aggtcggttt cgtagaggct gcctgcgccg gtggcggcgg aaagccagtt
      901 ggtcaggcgg cgggcaaggc ggctgtacac gtcgcctgct tcgggatggg gatcgtcgta
      961 gagatagacg aggtcgaggt cggaggcgta gccgagttct ttgccgccga gtttgccgta
     1021 gccgacaacg gcgaattgcg gcgtgtcgcg gtgttttttg ggcatatccg cccacgcgca
     1081 cgaaagggcg gcggcaatga cggtgtcggc gagggcggag agttggtcgg aaagggattc
     1141 taccgtccac agtccggcga ggtcttggac ggcgaggcgg aagacttggg cgtgctggaa
     1201 gcggcgcagg gtgtccattt gcgcttcggt gtcgccgccg caggcttcga tgccgtctga
     1261 aagggcggcg gcgagtttgt cccaatcaaa cgccgtatct aataattgcg cgctgatgag
     1321 ttcatccaac aaaatcggat atttgttcag atacgccgcc acccacgagc tttggctcat
     1381 aatctctgcc agttgcgcca gggtttgcgg atgttcgttg agaaaggcga gataggcgga
     1441 gcggcggctg atgttttcga ggaagtcaaa cagccgcatt aatgtatcgt tcggattggg
     1501 ttgctcggtc gccgcctgta cgaacaacgg cacaatcgcg tcaaaacgcg gctgggcgtg
     1561 tgcggaaagg tggcggtatt tatggccgtt gcggatttgg tcgagccgcg tagcgatagt
     1621 tttggcatca aacccatatt ctttcagacg gcctagccgt tcttcttcgt cgggtttttc
     1681 ctgccatgcc cattgccatt cgctgttgct ttgcgtttgc tcttcgggtt cgctcaagat
     1741 ttcgttaaac aactgattga ctttgttccg atgaacattg aggccgtctg aaaaagcagt
     1801 atagctgtcg aaacccatgc tttcggcgag cagttgttgc tgttcgggcg aagccggcag
     1861 ggtttgggtt tgccggtcgt cccagtattg caggcggtgt tcgacatcgc gcaaaaagcg
     1921 gtaggcggca agcagggttt cgacgttttc agacggcata atcccggttt cggcgagttt
     1981 ttccagcgtt tcctgcgtgc ctttcagttg cagcgcgcgc atttgcccgc cgcgtatcat
     2041 ctggaaaatc tgggcgataa attcgacttc gcggatgcca cccgcgccga gtttgatgtt
     2101 gtccgccatc ccttttttgc cgacttcgct gcggatttgg cggtgcagct tacgcatcgc
     2161 ctcatacgcg ccgtaatcca gatatttgcg gaacacgaag gggcgcacca gcgatttgat
     2221 tccgttcgga tacggcgtaa ccacgcggcc tttgcaccac gcataacgtt cccattcgcg
     2281 cccctgcgta atcaggtatt gctccagcgc ggtttcgctc aacaccagcg cgcccgaatc
     2341 accgtccggc cgcagccgca tatcgacgcg gaacacctgc ccatcggcgg taatgtcgtt
     2401 caacaatgca atcagtttct gcccgacttt ggtgaagaac tcctgattgc cgcgttcgcg
     2461 cctgccgtcg gtatcgcccg attcgggata gacgaaaatc aaatcgatgt cggaagacac
     2521 attcaactca tagccgcccg ccttacccat cgccaccaca ctcaaatgct gcggcgattt
     2581 ggtataacgc ccgatcggcg tgccgtacat atcccgataa taggcgtagg cgaaatccag
     2641 cgcggtattg acggcaaaat cggcaaacag cgtaatcgtg cgggttactt cgttcaaatc
     2701 gctgatgcgg tttatatcgc gcacgataat ctgcgacacc acataacggc gcaacccgcg
     2761 caactgccgc gccaactctt cctcgttttc ttccgcacgg attgcgtccc agtcggcaaa
     2821 ggcttggaaa tccgcttcgg tcaacacctt gtccaacatc ggcaggaaaa tttcgggctt
     2881 gagtttgccg gaatcaaggt tgcgggcgag aaacagggaa tggcggcggg cggcgtcgag
     2941 gcggttgtcg gacatttcag attccgtttg gaaggatgac gggaagatga tggcggattc
     3001 acttcaaatc aggacagggc gaggggatgc cgtaccggtt taaagtcaag ccactatatc
     3061 atagcaacct tcgatgccgt ctgaaacctt accccgacat ccagacggat gcctgccgat
     3121 aaaaaacagc ggcaaatgcc gccgtttcct gttcacacct cgccacgctc ttttaaaata
     3181 gcataagccg agacctttgc aaaaatagtc tgttaacgaa atttgacgca taaaaatgcg
     3241 ccaaaaaatt ttcaattgcc taaaaccttc ctaatattga gcaaaaagta ggaaaaatca
     3301 gaaaagtttt gcattttgaa aatgagattg agcataaaat tttagtaacc tatgttattg
     3361 caaaggtctc aagccgcact tttctcccgt gtggaatttt taaaaaacca tcactcttga
     3421 cgatttcatg cagctcctca cttgatttct ccgcatattc cattttcaac gccttatgct
     3481 cattggctgc ttcaatcatt ccctcgccta ctgcttttgc cacaccattc caaaaaccca
     3541 taatcatgtc ctccaatcgt ttcaaataaa taaggtcgtt gtttctgtca agaaacaacg
     3601 acagtttata aactgtcgga aaacaaaatg ccgtctgaag gccggataat gcttcagacg
     3661 gcatcggatt gccggtttta accctgccct ccgccgttgt tcaaatagtg ttcccagtga
     3721 gaatataacc gggcaatatt ttcttcatag gtgtgggcag aaggaatata accgtctttg
     3781 ggcggcatat acctgacaaa tgattctatt tcttccggat aatcaaaatc tgcataaatt
     3841 ttttcaactt cccccaaagg gtcgtcaaat tcttcctttc tatggaatac atcgcttaag
     3901 aggacgtaca gccaattttt aggggaataa tccctcattt ccgaagccaa atcttccaga
     3961 acaggcttta attcaagaat attggatttg ttgattaaag atagtttgaa cacacgttca
     4021 tcatgatctg aaagcgtcat ttttcgggca taagctgcca catcagccca acccaagtat
     4081 ttattcccat acccccacag aatatccttc caagaaagac ggactttttg cgcggttaaa
     4141 tcaaggttca ttttttactc cggttacctg ctccgtgttc acgcatcggt ttgccttatt
     4201 tcaaacaggg cggcgcgtaa gggattttca aggggcggca ggcggaaaaa ggaaatgaaa
     4261 atgccgtctg aacggcaaaa cgtgcgttca gacggcattt tgggcggatc gcgccaaagc
     4321 cttcaaacat tctttgacca atgccggacc tttgtatatc aatccgctgt acacttggac
     4381 ggcggtcgcg cccaagcgga ttttctctgc cgcgtccctg ccttccataa tgccgcctac
     4441 gccgataatc ggcagcttgc cgtcgatgtg gtctgccaac agcttcaaca cccgattact
     4501 tttttcatga acgggcagcc cgctcaaacc gccctgctcg cctgcgagcg gatggctgcc
     4561 gagacttgat ttgtcgatgg tggtattggt agcgatgatg ccgtccattt cgacggattt
     4621 gacaacgtgg gcgatgtctt cgatttgtgc ttcatccaaa tcgggggcga ttttgacggc
     4681 gagcgggacg tatttcccgt gtacagaggc aagctgtgcc tgtttgtttt tcaaagcctc
     4741 aagcaatgcg ctcaactcgt cgccaccttg cagcgcgcgg aggtttttag tgttgggcga
     4801 ggaaatattg acggtaatgt aacttgcgtg tgcgtaggct ttttcgaggc agattaaata
     4861 atcatcggca gcgttttcaa tcggtgttac agcgtttttg ccgatgttga tgcccaatac
     4921 gccgctgaat ttactttttt cgatgttgcg tatcatggtg tcgataccgt ggttgttgaa
     4981 acccatgcgg ttgatgatgc cttgatattc gggaacgcgg aagaggcgcg gctgcgggtt
     5041 gccgggctgc ggtttgggcg ttaccgtgcc gatttcgatg aaaccaaagc cgagcgcgcc
     5101 caatgcgtcg atgtattcgc cgtttttgtc gagtccagcg gcaagtccga cagggttggg
     5161 caaatccata cccatcaatt ttacaggttt ggtacggttg ccggttacgg gaatcagacc
     5221 caatttataa accgtgtaga gcgcgtcgag cgtgaagtgg tgggcttttt cggcatcgag
     5281 tgcaaacagg atgcggcggg caagtggata catgacaggg ctttctgctt cagatggaaa
     5341 tgccgccatt ttaaccgaaa tcccgccaaa catacggcac acaggtcaaa cttgaacgac
     5401 aaaacccgcc gcacccaaaa atgtacaaat cggcaaatat tgatataata gtcattagat
     5461 agaaaactct attaaaagtc ggtacaatac caccgattta tttcccttaa acaacctgcc
     5521 gcaaggcata aaggaacgac tgatatgtca aacatcgaac aacaagttaa aaaaattgtt
     5581 gctgaacaac tgggcgtaaa cgaagccgac gtgaaaaacg aatcttcttt ccaagacgac
     5641 ttgggcgcgg attctttgga caccgtggag ctggttatgg ctttggaaga agcattcggc
     5701 tgcgaaatcc ccgacgaaga tgccgaaaaa atcaccaccg tccaactggc tatcgactac
     5761 atcaatgccc acaacggcta accggtcgtc gcccgacaca acagcctctg ctgcaccgcg
     5821 caatagaggc ttttccctta tatggcaaac tgtttgaacc cctgccgcaa attacagacg
     5881 gcatctgcac gacagccgca tatgccccca ttccgacaac caacagcgag gttatcatga
     5941 gtcagagaag agtagtcatt acaggcttag gtcaggtttc ccctgtcggc aacactgtcg
     6001 cagaggcttg ggacaccctg ctcgccggca aaagcggcat cggcgcgatt acccgctttg
     6061 acgcatccga catcaacagc cgtgtcgccg gcgaagtgcg cggtttcgac atcggacaat
     6121 acatcagcgc gaaagaagcg cgccggatgg acgtattcat ccactacggc attgccgccg
     6181 cattgcaggc aatcgccgat tcgggcttgg acgatgtgga aaacctcgac aaagaccgca
     6241 tcggcgtgaa catcggttcc ggcatcggcg gactgcccag catcgaggtc accggcaaag
     6301 ccgtaatcga aggcggcgcg cgcaaaatca accctttctt tatccccggt tcgctgatca
     6361 acctgatttc cggacacgtt accatcctca aaggctaccg cggcccgagc tacgggatgg
     6421 tttccgcctg taccaccggc gcgcacgcca tcggcaattc cgcccgactg atcaaatacg
     6481 gcgacgcgga cataatggtt gccggcggcg cggaaggcgc aatcagcact ttgggcgtgg
     6541 gcggttttgc cgcgatgaaa gccctctcca cccgcaacga cgaccccgcc accgcttccc
     6601 gtccgtggga caaaggccgc gacggcttcg ttatcggcga aggcgcgggc atattggtgt
     6661 tggaagaatt ggaacacgcc aaaaaacgcg gcgcgaaaat ctacgccgaa atcgtcggct
     6721 tcggcatgag ttccgatgct taccatatca ccgcgccgaa cgaagaaggc cccgcccttg
     6781 ccgttacccg cgcgctgaaa gatgccggca tcaatcccga agacgtggat tacgtcaacg
     6841 cgcacggcac gtccaccccc ttgggcgatg ccaacgaaac caaagccctc aaacgcgcgt
     6901 tcggcgaaca cgcctacaaa accgtcgtca gctcgaccaa atccatgacc ggccacctgc
     6961 tcggcgcggc gggcggcgtg gaggccgtgt acagcatttt ggcgatacac gacggcaaaa
     7021 tcccgccgac catcaacatt tttgaacaag acgttgaagc cggctgcgat ttggactact
     7081 gcgccaacga agcgcgcgac gcggaaatcg acgttgccat ttccaactcc ttcggcttcg
     7141 gcggcaccaa cggcacgctg gtgttcaaac gcttcaaagg ctgattccgc aaagccgccg
     7201 ccgacatcga aatgccgtct gaaaccgttt cagacggcat ttttatagtg attaacaaaa
     7261 atcaggacaa ggcggcgagc cgcagacagt acaaatagta cggaaccgat tcacttggtg
     7321 cttcagcacc ttagagaatc gttctctttg agctaaggcg gggcaacgcc gtaccggttt
     7381 ttgttaatcc actataatac gcaggcaaac ccaagccttg ctgggttaca ggatgaaacc
     7441 ctgccgtagc agaccgcatt tccaatccat attcaaggtg tccgccttat gaaaatcgtt
     7501 tttatcacaa cagtcgcatc cagcatttac ggtttccgcg cccccgtcat taaaaaatta
     7561 atcggcaaaa accatcaggt gtatgccttt gtatcggagt tttccgataa tgaattggat
     7621 attatcaggg aaatgggggt tacacccgtt acctaccgtt caaaccgcag cgggctgaac
     7681 ccgttttccg atataaaatc caccttcctc atctttaaag aactcaaaaa aatatcgccg
     7741 gatttggttt tcccttattt cgcaaaaccc gtgattttcg gcacttttgc cgcaaaactg
     7801 gcaggcgtgc ccagaatcgt cgggatgctg gaaggtttgg gattcgcatt taccccgcag
     7861 ccggaaggca taccgttaaa aacaaaaatc ataaagggga ttttgattgc cttgtaccac
     7921 attgccctgc cgatgttgga aagcctgatt gtattaaacc ccgacgacaa agacgaactg
     7981 acggacaaat acggcatcaa aataaaaaac atccatattt tgggcggaat cggtctggat
     8041 ttgcggcaat atccttattc cgaggcggat attcccgatg aaaaagaacc cgtaaaattc
     8101 ctctttatcg gcagatttct gaaagaaaag gggattgatg attttattcg ggcggcggaa
     8161 caggttaagg acaaataccc cgatacggtt tttaccgctt tgggcgcaat cgacaagtca
     8221 cgcggggggt ggggggggga tttggaacgc cttgccgccc gcgatattat ccgtttcccc
     8281 ggttttgtga acaatgtttc cgaagtgata aaagaacatc atatattcgt attgccgtct
     8341 tattataggg aaggcgttcc ccgaagcact caggaggcaa tggccgtcgg cagggcagtg
     8401 attacgacgg atgtccccgg atgcagggaa acggtcgccg acaaggtcaa cggcttcctg
     8461 atcgagcctt ggaatccccg catcttggcc gaaaaaatga tttattttat cgaaaacagg
     8521 gaagccgtcc gcctgatggg gaatgcaagt tatgcgattg ccaaagataa attcgatgcc
     8581 gaaaaagtcg atttgaaatt gctcgatatt ttgaaggcgt aaacaaggct gcccgctttt
     8641 gagtttcggt catttctgat aaacccccgt cattcccgcc acacccccgt cattccctcg
     8701 aaagcgggaa ttcaggttcg tttagtttcg gtcatttccg ataaattctt gcaactttgc
     8761 gtttctagat tcccactttc gtgggaatga cg
//
LOCUS       DAWFHY010000084         8515 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.084, whole genome shotgun sequence.
ACCESSION   DAWFHY010000084 DAWFHY010000000
VERSION     DAWFHY010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.084"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(94..990)
                     /gene="argB"
                     /locus_tag="WGQ68_001725"
     CDS             complement(94..990)
                     /gene="argB"
                     /locus_tag="WGQ68_001725"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEZ0715491.1"
                     /translation="MAFENIISAADKARILAEALPYIRRFSGSVVVIKYGGNAMTEPA
                     LKEGFARDVVLLKLVGIHPVIVHGGGPQINAMLEKVGKKGKFVQGMRVTDKEAMDIVE
                     MVLGGHVNKEIVSMINTYGGHAVGVSGRDDHFIKAKKLLIDTPEQNGVDIGQVGTVES
                     IDTGLVKGLIERGCIPVVAPVGVGEKGEAFNINADLVAGKLAEELNAEKLLMMTNIAG
                     VMDKTGNLLTKLTPKRIDELIADGTLYGGMLPKIASAVEAAVNGVKATHIIDGRLPNA
                     LLLEIFTDAGIGSMILGGGEDA"
     gene            1159..2289
                     /locus_tag="WGQ68_001726"
     CDS             1159..2289
                     /locus_tag="WGQ68_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase family protein"
                     /protein_id="HEZ0715492.1"
                     /translation="MIKISTRFDAGSVVVKDLTDPSNIRLALRPDNASDFAQWFYFRL
                     QGAAYQNCIMHFENAAEAAYPKGWEGYQACASYDRRNWFRVPTSYENGVLTVNHTPLS
                     NSVYYAYFEPYSEEQHLNLLGDAQGSGLCRIDDLGSTVQGRDINLLTIGNQVESDLKI
                     WITARQHPGETMAEWFVEGLLGRLLDPQDPTARTLLDRATFYIVPNMNPDGSALGNLR
                     TNAAGANLNREWENPTVEKSPEVFFVRERMLETGVDLFLDIHGDEGLPFVFVAGTEGV
                     PNYNPRISALETQFKTALLNASPDFQDEYGYEKDAPGQANVTLATNWVGNRFNCLAYT
                     LEMPFKDNANLPDDDFGWNGQRSLRLGEAALSAILNVIGDLR"
     gene            2360..3211
                     /locus_tag="WGQ68_001727"
     CDS             2360..3211
                     /locus_tag="WGQ68_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEZ0715493.1"
                     /translation="MIIHPQFDPVLISIGPLAVRWYALSYILGFILFTFLGRRRIAQG
                     LSVFTKESLDDFLTWGILGVIFGGRLGYVLFYKFSDYLAHPLDIFKVWEGGMSFHGGF
                     LGVVIAIWLFGRKHGIGFLKLMDTVAPLVPLGLASGRIGNFINGELWGRVTDINAFWA
                     MGFPQARYEDAEAAAHNPLWAEWLQQYGMLPRHPSQLYQFALEGICLFAVVWLFSKKQ
                     RPTGQVASLFLGGYGIFRFIAEFARQPDDYLGLLTLGLSMGQWLSVPMIVLGIVGFVR
                     FGMKKQH"
     gene            complement(3294..3956)
                     /locus_tag="WGQ68_001728"
     CDS             complement(3294..3956)
                     /locus_tag="WGQ68_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecA family protein"
                     /protein_id="HEZ0715494.1"
                     /translation="MDSQKFTEASKRRLSELLDAKSEQGNTMRCDEVQGFMTALLSGP
                     DKLTPLDWLPEVLGDESQFTAAERSEIERLVLAMAMETTAAMSDKKLPDLWLYENEDG
                     GSDFYTWCNAYLYGLDIVPTDWFEAVDDEAFEELFYPIMALGGIYDEEENGAIRLQFT
                     EGELAELESELPYALADIYRYWQAVINKPQTVRREGEKTGRNDPCPCGSGRKYKACCG
                     KN"
     gene            complement(4051..5844)
                     /locus_tag="WGQ68_001729"
     CDS             complement(4051..5844)
                     /locus_tag="WGQ68_001729"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEZ0715495.1"
                     /translation="MQLDIDRLVAYFGGVNALAEALKQHDPENAATTAAIYKWRTRGS
                     LPLAQLQKLTALAEAQGRPLDLNAFLQKNESLERTEMTQANRVIIFDTTLRDGEQSPG
                     AAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVNAIAKTITKSTVCSLSRAIE
                     RDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVIEAAVKAVKIAREYTDDV
                     EFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYKTEEFFRELIAKTPNG
                     GKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNASVEEIVMALKVRH
                     DLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGIHQDGVLKHRET
                     YEIMSAESVGWSANRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFARFKELADKK
                     REIFDEDLHALVSDEMGNMNTESYKFISQKISTETGEEPRADIVFSIKGEEKRASATG
                     SGPVDAIFKAIESVAQSGATLQIYSVNAVTQGTESQGETSVRLARGNRVVNGQGADTD
                     VLVATAKAYLSALSKLEFSAAKPKAQGSGTI"
     gene            6089..7198
                     /gene="proB"
                     /locus_tag="WGQ68_001730"
     CDS             6089..7198
                     /gene="proB"
                     /locus_tag="WGQ68_001730"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEZ0715496.1"
                     /translation="MKYKRIVFKVGTSSITHSDGSLSRGKIQTITRQLAALHHAGHEL
                     VLVSSGAVAAGFGALGFKKRPVKIADKQASAAVGQGLLMEEYTANLSSDGIVSAQILL
                     SRADFADKRRYQNAGGALSVLLQRRAVPIINENDTVSVEELKIGDNDTLSAQVAAMIQ
                     ADLLVLLTDIDGLYTGNPNSNPDAVRLDKIEHINHEIIEMAGGSGSANGTGGMLTKIK
                     AATIATESGVSVYICSSLKPDALAEAADNQADGSFFVPRAKGLRTQKQWLAFYSESRG
                     SVYVDEGAEHALSEQGKSLLMSGIAGIEGHFSRMDTVTVYSKATKQPLGKGRVLFGSA
                     AAEDLLKSRKAKGVFIHRDDWISITPEIRLLLTEF"
     gene            7211..8473
                     /locus_tag="WGQ68_001731"
     CDS             7211..8473
                     /locus_tag="WGQ68_001731"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEZ0715497.1"
                     /translation="MSNTQKQLALAKAAKKSVNTADTEEKNRALLAMADSLEAAAADI
                     LAANRQDLEAAAGNIPESMTDRLLLDGKRICAMADGIRAVTALPDPVGEILETSTLPN
                     GLEIVKKRVAMGVIGIIYESRPNVTSDAAALALKSGSAVVLRSGKDAFQSARAIVAAL
                     KAGLAQTRIDPDALQLIENTGREGSYEMMRAKDYLDLLIPRGGAGLIRAVVENAVVPV
                     IETGTGIVHIYIDKDADWDKALRIVYNAKTSRPSVCNSMEVLLVHEDIAADFLPKLER
                     LLVRDRIEAGLPPVRFRLDPQAARHIGGEAAGADDFDTEFLDYILAVKTVASVEEAVG
                     HIEAHGTHHSDGIVTENRHAADYFTTHIDSAAVYVNASTRFTDGGEFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYIVQGTGQVRE"
BASE COUNT         1917 a         2405 c         2223 g         1970 t
ORIGIN      
        1 tcaggaatga cggtttggaa attgcccgaa accccaaaaa cagaaaccag acaaacaggt
       61 tttccgccaa agccggcatt ttccgacttt gcttcaggca tcttccccac cgcccaaaat
      121 catcgaaccg ataccggcat cggtaaagat ttccagcaaa agcgcgttgg gcaacctgcc
      181 gtcgatgata tgcgtggctt tcacaccgtt gacggcggct tcgaccgcag aagcgatttt
      241 cggcagcata ccgccataca gcgtgccgtc ggcaatcagt tcatcaatcc gtttcggcgt
      301 gagtttggtc agcagattgc ccgttttgtc catcacaccg gcgatattcg tcatcatcaa
      361 gagtttttcg gcgttcaatt cttccgccaa tttgcctgct accaaatcgg cgttgatgtt
      421 gaacgcttcg cctttttcac ctacgccgac gggggcgacg acgggaatgc agccacgttc
      481 tatcagccct ttaaccaaac cggtatcgat gctttccacc gtaccgacct gtccgatgtc
      541 cacgccattc tgttcgggcg tatcgatcaa aagtttcttc gccttaatga aatggtcgtc
      601 gcgtccgctc acgccgaccg cgtgcccgcc atatgtgtta atcatcgaca cgatttcttt
      661 attgacgtgc ccgcccaaca ccatttcgac aatatccatc gcctctttgt cggtaacgcg
      721 cattccttgg acaaacttac cctttttgcc gactttttca agcatcgcat tgatctgcgg
      781 cccgccgcca tgaacgatga cgggatgaat gccgaccagc ttcagcagca cgacatctcg
      841 ggcaaaccct tctttcaagg caggttcggt catggcgttg ccgccgtact tgatgacgac
      901 gaccgaaccg gaaaaccggc ggatgtaagg cagtgcttcg gcaaggatac gcgccttgtc
      961 ggcggcggaa ataatgtttt caaacgccat aacggctccc tattgttgac aatttcggct
     1021 tatgttaacg ccaaacgccg tctgaaacaa tcttcagacg gcatccggca caccttcggg
     1081 caggttgaag ccttgcgata cgggcggtaa aatcgcggtt tatccggttc gagaaaccgg
     1141 cgacagcaag gattccccat gattaaaatc agcacccgct tcgatgccgg ctcggtcgtt
     1201 gtcaaagacc tgaccgatcc ttccaacatc cgcctcgccc tgcgtcccga caacgcctcc
     1261 gattttgcac aatggttcta cttccggctg caaggcgcgg catatcaaaa ctgcatcatg
     1321 cattttgaaa acgcggcaga agccgcctac ccgaaaggct gggaaggcta tcaggcgtgt
     1381 gcctcatacg accgccgcaa ctggttccgc gtaccgactt cctacgaaaa cggcgtactg
     1441 accgtcaatc atacgccgct gtccaacagc gtgtattacg cctatttcga accttactcc
     1501 gaagaacagc atttaaacct cctcggcgac gcgcaaggca gcggcctgtg ccgcatcgac
     1561 gacttgggca gcaccgtgca agggcgcgac atcaatctgc tgaccatagg caaccaggtc
     1621 gaaagcgatt tgaaaatctg gattaccgcc cgccagcatc cgggcgaaac catggcggaa
     1681 tggtttgtcg aaggactgct cggcaggctg ctcgatccgc aagacccgac agcacgcacc
     1741 ctgctcgacc gcgccacgtt ctatatcgtc cccaatatga accccgacgg ttcggcactg
     1801 ggcaaccttc gtaccaacgc cgccggtgcg aacctcaacc gcgagtggga aaacccgact
     1861 gttgaaaaaa gccccgaagt gttcttcgtg cgtgaaagaa tgttggaaac cggcgtggat
     1921 ttgtttttgg atatccacgg cgacgaaggg ctgccctttg tctttgttgc aggaactgaa
     1981 ggcgtgccga actacaatcc acgcatttcc gcgttggaaa cacagttcaa aaccgccctt
     2041 ttgaacgcca gccccgattt ccaagatgaa tacggttacg aaaaagatgc gcccggtcag
     2101 gcaaacgtga ccttggcaac aaactgggtc ggcaaccgtt tcaactgcct tgcctatacg
     2161 ttggaaatgc cgttcaaaga caatgccaac ctgcccgacg acgacttcgg gtggaacggt
     2221 caacgttcgt tgcgcctggg cgaagccgcg ttgtccgcga tcctcaacgt catcggcgat
     2281 ttgcgctgaa acccttattg cagaaatgcc gtctgaagca tcatgccttt cagacggcat
     2341 ttgaaaacga aagcctgtta tgattatcca tccccaattc gatcccgtcc tcatcagtat
     2401 cggcccgctt gccgtccgct ggtatgccct aagctacatc ctcggattta ttctttttac
     2461 ctttctcggc agaaggcgca tcgcgcaagg cttgtccgtt tttaccaaag aatcgcttga
     2521 cgacttcctc acttggggca ttttgggcgt aatttttggt gggcgtttgg gttacgtcct
     2581 gttttacaag ttttccgact acctcgccca tccgcttgat attttcaagg tatgggaagg
     2641 cggaatgtcg ttccacggcg gctttttggg tgtagttatt gccatatggt tgttcggccg
     2701 caaacacggc atcggcttcc tcaaactgat ggacacggtc gcaccgctcg ttccgctggg
     2761 tctcgcttcg ggacgtatcg gcaacttcat caacggcgaa ctttggggac gcgttaccga
     2821 catcaacgca ttttgggcaa tgggcttccc gcaggcgcgt tacgaagatg ccgaagccgc
     2881 cgcgcacaat ccgctttggg cagaatggct gcaacaatac ggtatgctgc cgcgtcatcc
     2941 ctcgcagctt tatcagtttg cacttgaagg catctgcctg ttcgccgtcg tttggctgtt
     3001 ctctaaaaaa cagcggccga ccggacaagt cgcctcgctc ttcctcggcg gctacggcat
     3061 attccgcttc attgccgaat tcgcacgcca acccgacgac tatctcgggc tgctgacctt
     3121 ggggctgtcg atggggcaat ggttgagcgt cccgatgatt gttttgggta tcgtcggctt
     3181 tgtccggttc ggcatgaaaa aacagcactg accgcctgtg aaaaataccg tctgaaacat
     3241 ggaaatccgg tttcagacgg catttttacg tttggttcat ggaaacaaac gcttcaattc
     3301 ttaccgcaac acgccttgta ttttctgccg ctgccgcacg gacagggatc gttcctgcct
     3361 gttttttcgc cttccctgcg gacggtttgc ggtttgttga tgactgcctg ccagtagcgg
     3421 tagatatccg ccaatgcata aggcaactcg gattccagtt ccgccagctc gccttctgtg
     3481 aattgcagac ggatagcgcc gttttcctct tcgtcgtaaa taccgcccaa tgccatgatg
     3541 ggataaaaca actcttcaaa cgcttcatca tcgacggctt caaaccaatc ggtcggcaca
     3601 atatccaaac cgtaaagata agcattgcac catgtgtaaa aatcgctgcc gccgtcttcg
     3661 ttttcataca gccacaaatc gggcagtttt ttatccgaca tcgcggcggt tgtttccatc
     3721 gccattgcca aaaccagccg ttcgatttcg gaacgttcgg cggcggtaaa ttgcgattcg
     3781 tcgcccaaca cttcgggcag ccagtcgagc ggtgtcaatt tgtccggccc gctcaacagc
     3841 gccgtcataa aaccttgaac ctcgtcgcaa cgcatcgtgt tgccttgttc gcttttggca
     3901 tccaacaatt cgctcaaccg ccgtttggat gcttcggtaa atttttggga atccatgatt
     3961 ttccttttca aatgggtttt gcgccctatt atcgccgcaa tgccgtctga agccttttta
     4021 tgcttcagac ggcatcgtct gttttcacgc tcaaatcgta ccgctgcctt gcgctttcgg
     4081 tttggcggca ctaaattcca gtttgctcaa agcggaaagg taggctttgg cagtggcaac
     4141 caaaacgtcg gtatccgcgc cctgaccgtt gacgacgcgg ttgccgcgtg ccagacggac
     4201 gctggtttcg ccttggcttt ccgtaccttg cgtgacggca ttgacggaat aaatctgcaa
     4261 agtcgcgccg ctttgcgcca cgctttcaat cgctttgaaa attgcatcaa ccgggccgga
     4321 accggttgcg gaagcgcgtt tttcttcgcc tttgatgctg aacacgatgt cggcgcgcgg
     4381 ctcttcgccg gtttcggtac tgattttttg ggaaatgaat ttgtagcttt cggtattcat
     4441 attgcccatt tcgtccgaca ccagcgcgtg caggtcttca tcgaagattt cgcgtttttt
     4501 gtcggcgagt tctttgaagc gtgcaaatgc cgcgttcagt gcctcttcgc tttccaactc
     4561 gatgcccaaa tccgccagct tggttttgaa ggcgttgcgg ccggacaatt tgcccaagct
     4621 caatcggtta gccgaccaac cgaccgattc ggcagacata atctcgtaag tttcgcggtg
     4681 tttcagcacc ccgtcctgat ggatgcccga ttcatgcgaa aaggcattgg caccgacaat
     4741 ggctttgttg ggctgcacgg gatagcccgt aatggtggac accagtttgg acgaaggcac
     4801 gatttgcgtg gtatcgatgc cggtttccaa gccgaacaag tcgtggcgca ctttcaacgc
     4861 catcacgatt tcttcaaccg aagcattgcc tgcacgttcg cccaagccgt tgacagtaca
     4921 ttccacctga cgcgcgccgc cttttaatgc ggcaagcgaa ttggcaaccg ccaagcccaa
     4981 atcattgtgg cagtgtgccg accaaacgac tttgccgccg ttgggcgttt tggcaatcag
     5041 ttcgcggaaa aattcttcgg ttttgtacgg gattgaatag ccgacggtat cgggaatatt
     5101 gatggtggtc gcgcccgctt caatcaccgc gccgcagatt tcggcaagga aatcgatttc
     5161 cgaacgcaac gcgtcttcgc aggaaaattc cacatcgtcg gtgtattcac gagcgatttt
     5221 caccgctttg accgccgcct caatcacctg cttcggcttc atcttcaatt tgtactccat
     5281 atggatgggg ctggtggcga tgaaggtgtg gatgcgtttt ttcggcgcgg gcgcaacggc
     5341 ctcacccgcc tgacggatgt cccgctcgat ggcgcgggac aatgaacaga ccgttgattt
     5401 ggtaatggtt ttcgcaatcg cattgaccgc ctcgaaatcg cccgggctgg cagcggcaaa
     5461 acccgcttcg atgatgtcca cacccaattt ttccagctgg cgggcgacgc ggattttttc
     5521 ctctttggtc atagcggcgc cgggcgactg ctcgccgtcg cgcagggtgg tgtcgaaaat
     5581 gataacgcgg ttggcctgtg tcatttctgt tctctccaga gattcgtttt tttgtaaaaa
     5641 agcattcaaa tccagcggcc tgccttgcgc ttccgccaac gcggtcagct tttgcagttg
     5701 cgccagaggc agcgagccgc gcgtgcgcca tttatagatg gcggcggtcg tcgcggcatt
     5761 ttcgggatcg tgctgtttca acgcttcggc aagcgcgttc acgccgccga aataagcaac
     5821 caagcggtct atatctaatt gcatggtagt ccttgtctaa aataaatcag cctacagcag
     5881 aaatatggac aggattatac gcattttgga caaaacggca atataaattt gaaaaatctc
     5941 aataagccgc catagatttt attaatttta tacattttgt ccattttatt gcagacgcgg
     6001 cgtgattgtt gacaattgcg gcagaacagg gcaaattcgg cggcacaagg tggcaacgag
     6061 acaggacaaa ccaacaagga cggaaacaat gaaatacaaa agaatcgtat ttaaagtcgg
     6121 cacatcttcg attacccatt cggacggcag tctctcgcgc ggcaaaatcc aaaccatcac
     6181 ccgccagctt gccgcattgc atcatgcggg acacgagctg gtcttggtgt cttccggcgc
     6241 ggttgcggca gggttcggtg cgctgggttt caaaaaacgt ccggtcaaaa tcgccgacaa
     6301 acaggcttcc gccgccgtcg ggcaggggct gctgatggaa gaatatacgg caaacctgtc
     6361 ttcagacggc atcgtgtccg cacaaatcct gctcagccgc gccgactttg ccgacaaacg
     6421 ccgctaccaa aatgccggcg gcgcactttc cgtgctgctg caacgccgcg ccgtccccat
     6481 catcaatgaa aacgatacgg tttcggttga ggaattgaaa atcggcgaca acgacacatt
     6541 gagtgcgcaa gtggcggcga tgatacaggc agacctcttg gtgctgctga ccgacataga
     6601 cggtctttac acgggcaacc cgaacagcaa tcccgatgcc gtacggctgg acaaaatcga
     6661 acacatcaac catgaaatca tcgaaatggc gggcggctcg ggttcggcaa acggcacagg
     6721 cggtatgctg actaaaatca aagcggcgac gattgcgacc gagtccggcg tatcagtcta
     6781 tatctgttcc tcgctcaaac ccgatgcact tgccgaagcg gcagataatc aggcggacgg
     6841 ctcgtttttc gtcccccgtg ccaaaggttt gcggacgcag aagcaatggc tggcgttcta
     6901 ttccgaaagc cggggcagcg tttatgtgga cgaaggtgcg gaacacgctt tgtccgaaca
     6961 ggggaaaagc ctgctgatgt cgggcattgc cggaatcgaa gggcattttt cccgtatgga
     7021 caccgtaacc gtgtacagca aggcaaccaa acagcccctg ggcaaagggc gcgtcctgtt
     7081 cggttctgcc gccgccgaag acctgctcaa atcgcgtaag gcgaaaggcg tgttcatcca
     7141 tcgggacgat tggatttcca tcacgcccga aatacgcctg cttctgaccg aattttagaa
     7201 aaggaaaccc atgtcaaaca cacaaaaaca gcttgccctt gccaaagcgg caaaaaaatc
     7261 cgtcaacacg gcggatacgg aagaaaaaaa ccgcgcgctg cttgctatgg cggacagtct
     7321 ggaagcggcg gcggcagata ttttggcggc aaaccgtcaa gacttggaag ccgcggcagg
     7381 caacattccc gaaagcatga ccgaccgcct tttgttggac ggcaaacgca tttgcgcgat
     7441 ggcggacggc attagggcgg ttaccgcact gcccgacccc gtgggcgaaa tactggaaac
     7501 ctcgactctg cccaacggct tggaaatcgt caaaaagcgt gtggcgatgg gcgttatcgg
     7561 cattatttac gaaagccgcc cgaacgttac gtccgatgcg gcggctttgg cactgaaaag
     7621 cggcagcgcg gtcgtactcc gcagcggcaa agatgcattc caatccgcac gcgccatcgt
     7681 tgccgcactc aaagccggtt tggcgcaaac ccgcatcgac cccgacgcgc tccagttgat
     7741 tgaaaacaca gggcgtgagg gcagttacga aatgatgagg gcgaaagatt atctagacct
     7801 gctgattccg cgcggcgggg cggggctgat acgggcggtg gttgaaaatg ccgtcgtgcc
     7861 ggtcattgaa acgggaacgg gcattgtcca catttatatc gacaaggacg ctgattggga
     7921 taaggcactc cgtatcgtgt acaacgccaa aaccagccgt ccgtccgtgt gcaactcgat
     7981 ggaagtgctg ctggtgcatg aagacattgc tgccgacttc ctgcccaagc tcgaacgact
     8041 gttggttcgc gaccgcatag aagccggact gccgcccgtc cgcttccgtt tggatccgca
     8101 ggcggcgcgg catatcggcg gcgaagcggc gggtgcagac gatttcgata ccgagttttt
     8161 agactacatc ctcgccgtga aaaccgtcgc atcggtcgaa gaggcggtcg ggcacatcga
     8221 agcccacggc acacaccatt cagacggcat cgttaccgaa aaccgccacg ctgcggacta
     8281 tttcacgacc catatcgatt ctgccgccgt gtatgtcaac gcgtccacgc gctttaccga
     8341 cggcggcgaa ttcggcttgg gttgcgaaat gggcatctcc acgcaaaaac tccacgcccg
     8401 gggtccgatg ggtttgaaag agctgacgag ttacaaatac atcgtacaag gcacgggaca
     8461 ggttagggaa taatccgaat tcaaacgtct gatatagtgg attaacttta aaccg
//
LOCUS       DAWFHY010000085         8293 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.085, whole genome shotgun sequence.
ACCESSION   DAWFHY010000085 DAWFHY010000000
VERSION     DAWFHY010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8293
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.085"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(82..1338)
                     /locus_tag="WGQ68_001732"
     CDS             complement(82..1338)
                     /locus_tag="WGQ68_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEZ0715498.1"
                     /translation="MNAAQLDHTAKVLAEMLTFKQPADAVLSAYFREHKKLGRQDRHE
                     IAETAFAALRHYQKISTALRRPHAQPRKAALAALVLGRSTNISQIKDLLDEEETEFLG
                     NLKARKTEFSDGLNTAAELPQWLVEQLQQHWNEEEILAFGRSINQAAPLDIRVNTLKG
                     KRDKVLPLLQAESADAEATPYSPWGIRLKNKIALNKHELFLDGTLEVQDEGSQLLALL
                     VGAKRGEIIVDFCAGAGGKTLAIGAQMANKGRIYAFDIAEKRLANLKPRMTRAGLTNI
                     HPERISSEHDTRIARLAGKADRVLVDAPCSGLGTLRRNPDLKYRQSAETVANLLEQQH
                     SILDAASKLVKPQGRLVYATCSVLPEENELQVERFLSEHPEFEPVNCAELLQSLKVDL
                     DTGKYLRLDSARHQTDGFFAAVLQRK"
     gene            complement(1373..2230)
                     /locus_tag="WGQ68_001733"
     CDS             complement(1373..2230)
                     /locus_tag="WGQ68_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1853 family protein"
                     /protein_id="HEZ0715499.1"
                     /translation="MNYALDALWWKLTSQPVRDLASLLTAPPLWQSGCELGVRELLGE
                     RGFRYLLALDADSAPLTDYLARRAPFGHRLGIYAEELLAFWFANAPHAELLAHNLTVS
                     GSDGNTQGAADFVVRLNGKPYHIELTCKYYGGGTGRLEDMRGLDPKDTLLGKAAKLTA
                     QLGLPHTSDGIRTLRQHGLPLDVKPVSIVRGIGFFPHGFHAFEPPLNPYGWRGIYIQD
                     WAEYGFKRQEVRYHLLDRMAYLAPARVAETETLNATEIRRIDQGLIAVLECRPDGFWH
                     EIERIMKAV"
     gene            complement(2292..3107)
                     /locus_tag="WGQ68_001734"
     CDS             complement(2292..3107)
                     /locus_tag="WGQ68_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEZ0715500.1"
                     /translation="MTTLKPALPAYLDNIRIILTRTSHPANIGSAARAMKTMGLHKLT
                     IVAPNLMATPMTENPPVFDPEHPQSFKLPEESFILASGAADVLENATIAASLDEALAD
                     TTIACALTSRRREITAPLQTPRDLVSELLQTANRGEKVALVFGNETFGLSIEEVQACN
                     RLMTINGNPDYFSLNLAQAVQVVCYEIFSQTGSPMTHLQQEDHAATHEQIKGMVAHME
                     SVMNDIGFFNRRNGERLMRRMQSLFGRANTQTEDIDILRGFFNTVSHRIHKKD"
     gene            3171..3341
                     /locus_tag="WGQ68_001735"
     CDS             3171..3341
                     /locus_tag="WGQ68_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715501.1"
                     /translation="MPSEMFFNNVGNLTLRLKQTRVGRYFTRFGRIYPNQINSDFAAP
                     KDCASEPAVCHP"
     gene            3351..4136
                     /locus_tag="WGQ68_001736"
     CDS             3351..4136
                     /locus_tag="WGQ68_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HEZ0715502.1"
                     /translation="MNPFLNTAFKAARRAGQMMIRAAGNLDAVKTDSKAFNDFVSDVD
                     RNSEIILVEALKEAYPHHKITCEESGSHGKAAAEYEWIIDPLDGTTNFLHGHPQYAIS
                     MALLHKGVLQEALVYAPERNDVYMASRGKGALLNDRRIRVSNRIELNRCLIGTGFPVV
                     DQSMMDKYLAILKDFLSKTAGGRREGAASLDLCAVATGRFDGFFEFNLKPWDIAAGAL
                     IVQEAGGIVTDMTGEDAWLESGDIVAANPKVLAQMLKIISAHV"
     gene            4405..6525
                     /locus_tag="WGQ68_001737"
     CDS             4405..6525
                     /locus_tag="WGQ68_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0715503.1"
                     /translation="MGKANAFTAPITVVNYDEQALNNTEARTLVDAVAKKDASVWQFG
                     GESNTLTGLYFRGYQLDARQFSVNGLAGMYGTQGTAGVQVGSAQLIKGASTAVNGMDP
                     EGAVSGSVNIETKKAADEGNRKIGLGRFSNNRAQGTFDLGQRFGENKEFGVRANGKLR
                     HGDTPRHGYSEDNKEFALNADYRGETLRVAFDSIYAKRKTNGGRARIQDIQNAGGRLF
                     GAPDGKTNLLPSWNWQNTAGQTNMLTFEWDAFDNAQITGGIGYNKARYYGTLISPTVC
                     LNATSTCTDMQNTNASGKVTARNYDYNTGTARLTDQYFRTLSMNLTARGEFETGPVTH
                     NWSMAFDRVIRQRKTINGSGNGNSKIEVKANENIANQLASFTADYPNSWENSANLDAN
                     IKVNSLALSDTLGFVDNKYRLTLGERFQTVEYTDKKSQSGDAKRFSPMLMAAWVPQPD
                     LVVYGNYMEDLEPADIKTDDSGETTMAKPRVSRQFEVGVRKNWGNFVTTLNAFQIKRP
                     GYWRGHTTTKTTKGVTTTLTYGNNSDFARYKAQGGAAGDEQGMERNRGIEFNTYANLL
                     NNTLRPTLGLMYLQSTVKEYPNSRDMLVNGVQVANPRVIAKAGLEWDTPFAKGLTLNG
                     NVSYFGKSYQDTQKQYAFPSYTLIDVGARYKTKLGKNTLTVSSSVENLFNKNYWQVQR
                     GQYDRSFAVVGLPRTYWLKAELDF"
     gene            6649..8199
                     /locus_tag="WGQ68_001738"
     CDS             6649..8199
                     /locus_tag="WGQ68_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HEZ0715504.1"
                     /translation="MKKPLISVAAALLGVALGTPYYLGVKAEESLTQQQKILQEAGFL
                     TVESHQYERGWFTSTETTVIRLKPELLNNARKYLPDNLKTVLEQPVTLVNHITHGPFA
                     GGFGTQAYIETEFKYAPETEKVLERFFGKQVPVSLANTVYFNGSGKMEVSVPAFDYEE
                     LSGIRLHWEGLTGKTVYQKGFKSYRNSYDAPLFKIKLADKGDAAFEKAHFDSETSDGI
                     NPLALGSSNLTLEKFSLEWKEGVDYNVKLNELVNLVTDLQIGAFINPNGSIAPSKIEV
                     GKLAFSTKTGESGAFIDSEGQFRFGTLVYGDEKYGPLDIHIAAEHLDASALTVLKRKF
                     AQISAKKMTEEQIRNDLIAAVKGEASGLFTNNPVLDIKTFRFTLPSGKIDVGGKIMFK
                     DLKKEDLNQLGLMLKKTEADIRMSIPQKMLEDLAVSQAGNIFSVNAEDEAEGRASLDD
                     INETLRLMVDSTVQSMAREKYLTLNGDQIDTAISLKNNQLKLNGKTLQNEPEPDFDEG
                     GMVSEPQQ"
BASE COUNT         2000 a         2049 c         2307 g         1937 t
ORIGIN      
        1 tttttgttaa tccactataa ttgatgaaat gccgtctgaa cgctttgaaa agggttcgga
       61 cggcattttg ttcaaaccgg tttatttgcg ttgcaatacg gcggcgaaga agccgtcggt
      121 ttggtgtcgg gcggaatcga ggcgcaggta ttttccggta tccaaatcaa ccttcaggct
      181 ttgcagcagt tcggcgcagt tgacgggttc aaattcggga tgttcggaca ggaaacgttc
      241 gacttgcagc tcgttttctt ccggcaacac gctgcaggtg gcgtacacca agcggccttg
      301 cggtttcacc agtttggatg cggcatcgag gatgctgtgt tgctgttcca aaaggttggc
      361 cacggtctcg gcggattggc ggtatttgag gtcgggattg cggcgcagcg tgcctaagcc
      421 ggagcagggc gcatccacta aaacgcggtc ggctttgcct gccagtcggg caatacgggt
      481 atcgtgttca ctgctgatgc gttcagggtg gatgttggtc agtccggcac gggtcatgcg
      541 cggtttgagg ttggcaaggc gtttttcggc gatgtcgaag gcgtagattc tgcctttgtt
      601 cgccatttgc gcgccgatgg cgagggtttt gccgcctgcg ccggcacaga aatcgacaat
      661 gatttcgccg cgttttgcgc ccaccaataa agcaagcagc tggctgcctt cgtcttggac
      721 ttccagtgtg ccgtccaaaa acagctcgtg tttgttaagc gcgattttgt ttttcaggcg
      781 gatgccccaa ggcgaataag gcgttgcctc cgcatcggca ctttcggctt gcaacaatgg
      841 cagcacttta tcgcgtttgc ctttcaaggt attgacgcgg atgtcgagcg gcgcggcttg
      901 gttgatgctg cgtccgaagg cgaggatttc ttcttcgttc caatgctgtt gcagttgttc
      961 gaccagccat tgcggcaatt ctgcggcggt attcagaccg tctgaaaact cggttttgcg
     1021 ggctttcaaa ttgccgagga actctgtttc ttcttcatca agcaggtctt tgatttggct
     1081 gatgttggtg cttctgccga gaaccagtgc ggcgagtgcg gctttgcgcg gctgcgcgtg
     1141 aggacgacgc agggcggtac tgattttttg atagtggcgc agcgcggcaa aggcagtttc
     1201 ggcgatttcg tggcggtctt ggcggccgag ttttttgtgt tcgcggaaat aggcggagag
     1261 gacggcatcg gcaggctgtt tgaaagtcag catttcagcc aaaactttgg cggtatggtc
     1321 gagttgtgcg gcgttcatca ttaatgggtt ctcttaaagt ggggaaaggg tttcagacgg
     1381 ccttcataat gcgttcgatt tcgtgccaaa agccgtccgg ccgacattcc aaaacggcaa
     1441 tcaagccttg gtcgatacgg cggatttcgg ttgcgttcaa tgtttcggtt tcggcgacac
     1501 gcgcaggcgc gaggtaggcc atacggtcga gcagatggta gcggacttct tggcgtttaa
     1561 acccgtattc cgcccaatct tgaatataga tgccgcgcca accgtatgga ttaagcggtg
     1621 gctcaaaagc atggaaaccg tgtggaaaaa atccgatgcc gcgcacgatg gaaacgggtt
     1681 ttacgtcaag cggcagaccg tgttgccgca aggttcggat gccgtctgaa gtgtgcggca
     1741 gaccgagttg ggcggtcagt ttggcggctt ttcccaacag cgtgtctttg gggtcgagtc
     1801 cgcgcatgtc ttcgagcctg cccgtgccgc cgccgtaata tttgcaggtc agctcgatat
     1861 ggtagggctt gccgttaagc cttaccacaa aatccgccgc gccttgcgta ttgccgtccg
     1921 aaccggaaac cgtgaggttg tgcgcgagca gttcggcgtg tggcgcgttg gcaaaccaaa
     1981 atgccagcag ttcttcggcg taaatgccca agcggtgtcc gaacggggcg cgtcgggcga
     2041 ggtaatccgt cagcggagcg gaatcggcat ccaatgccaa aagatagcgg aaaccgcgtt
     2101 cccccaatag ttcgcgcacg cccaattcgc aaccgctttg ccacaaaggc ggcgcagtca
     2161 gcagcgaggc gaggtcgcgg acgggttggc tggtgagctt ccaccataat gcgtctaggg
     2221 cgtaattcat gcttatccga atatcagtca tgccgtctga aaagctggaa atgttttcag
     2281 acggccttaa atcagtcttt tttatggatg cgatggctga cggtattgaa aaaaccgcgc
     2341 aggatgtcga tgtcttcggt ttgcgtgttg gcgcgtccga acaggctctg catacggcgc
     2401 atcagacgct cgccgttgcg gcggttgaaa aagccgatgt cgttcatcac gctttccatg
     2461 tgggcgacca tgcctttgat ttgctcgtgg gtcgcagcgt ggtcttcttg ttgaagatgg
     2521 gtcatgggcg aaccggtttg gctgaagatt tcgtagcaca cgacctgcac ggcttgggcg
     2581 aggttgagcg agaaatagtc gggattgccg ttgatggtca tcagtcggtt gcaggcttgg
     2641 acttcttcga tgctcaagcc gaaagtctcg ttgccgaaaa ccagtgccac tttctcgcct
     2701 cggtttgcgg tctgcagtaa ttcggatacc aaatcgcgcg gggtttgcag cggcgcagta
     2761 atttcgcggc ggcggctggt cagggcgcag gcgatggtgg tgtcggcaag ggcttcgtcc
     2821 aaagaagcgg caatggtggc attttccaaa acgtctgccg cgccggaagc gaggatgaag
     2881 ctttcttccg gtaatttaaa cgattgagga tgctccgggt caaacacggg cgggttttcc
     2941 gtcatcggcg ttgccatcag atttggggcg acgatggtca gtttgtgcag acccattgtt
     3001 ttcatcgcgc gcgcggcaga gccgatgttg gcgggatggc tggtgcgcgt gaggatgatg
     3061 cggatgttgt ccagataagc gggcagggcg ggtttgagag tagtcatgtt cggttttcgg
     3121 tcggaatgga atagggtata atgcgccgtc tttttcgttc ggacggtaaa atgccgtctg
     3181 aaatgttctt taacaacgtc ggaaatttaa cgttgcgcct gaagcagaca cgcgtcgggc
     3241 ggtattttac ccgatttggc aggatttacc ccaaccaaat aaactccgat tttgccgctc
     3301 cgaaggactg cgcgtccgaa ccggcggtat gccaccctta aggaaatctg atgaatccgt
     3361 ttttgaatac agcctttaaa gccgcccgcc gtgccggtca gatgatgata cgcgccgcag
     3421 gtaacctcga tgccgtcaaa accgacagca aagccttcaa cgattttgtt tccgatgttg
     3481 accgcaattc cgaaatcatc ctggttgagg ctttgaaaga agcctatccg caccacaaaa
     3541 tcacttgtga agaaagcggc tcccacggca aagccgcagc cgagtacgaa tggattatcg
     3601 atccgctcga cggcacgacc aatttccttc acggtcatcc ccaatacgcc atctctatgg
     3661 cgctcctgca caaaggcgtg ttgcaagaag ctttggttta cgcccccgaa cgcaacgacg
     3721 tatatatggc ttcgcgcggc aaaggcgcgt tgctcaacga ccgccgcatc cgcgtttcca
     3781 accgcatcga attgaaccgc tgcctgatcg gtaccggctt ccctgttgtc gatcaaagca
     3841 tgatggacaa gtatctggcg attttgaaag atttcttgtc aaaaaccgcc ggcggccgtc
     3901 gtgaaggcgc ggcttctttg gatttgtgtg ctgtagcaac aggtcgtttt gacggctttt
     3961 tcgagtttaa cctcaaaccg tgggacattg ccgcaggtgc attgattgtt caggaagcgg
     4021 gcggtatcgt aaccgatatg acgggtgagg acgcttggtt ggaaagcggc gatattgtcg
     4081 ccgccaatcc caaagtattg gcgcagatgc tgaaaatcat ttccgcacac gtttaaattt
     4141 cagccctttc ccgaaaatat gggaaaacgg gcgggtatgc cgtctgaagt atcgcgccga
     4201 tatttcagac ggcattttta ttggcaacgc ggcaggacgg cggaaacggt caaatgcccc
     4261 aaactgccga ggatggaaac ccatatctga ttaaacggag aaccctgtgc gatttgttta
     4321 tagtggatta acaaaaacca gtacggtgtt gcctcgcctt agctcaaaga gaacgattct
     4381 ctaaggtgct gaagcaccaa gtgaatgggc aaagccaacg cctttaccgc accgattacc
     4441 gtcgtcaatt acgacgaaca agccctcaat aacaccgaag cgcgtacttt ggtggatgcc
     4501 gtagcgaaaa aagacgcttc cgtttggcag ttcggcggcg aaagcaacac gctgaccggc
     4561 ctgtatttcc gcggttatca gcttgatgcg cgccaattca gcgtcaacgg cttggccggt
     4621 atgtacggca cgcaaggcac cgccggcgtg caagtcggct ccgcgcaact gattaaaggc
     4681 gcgtccaccg ccgtaaacgg tatggacccc gaaggcgcgg tctccggttc cgtcaatatc
     4741 gaaaccaaaa aagccgccga tgaaggcaac cgcaaaatcg gtttgggccg gtttagcaac
     4801 aaccgcgccc aaggtacgtt cgacttgggt caacgcttcg gcgaaaacaa agaattcggc
     4861 gtgcgcgcca acggcaaact gcgccacggc gacactccgc gccacggtta cagcgaggac
     4921 aacaaagaat ttgccttgaa tgccgactat cgcggcgaaa cactgcgcgt ggcgttcgac
     4981 tccatctacg cgaaacgcaa aaccaacggc ggccgtgcgc gtattcagga tattcaaaac
     5041 gccggcggac gcttgtttgg cgcgcccgat ggtaaaacca acctgctgcc ttcttggaac
     5101 tggcaaaata ccgccggcca aaccaatatg cttacgtttg aatgggatgc gtttgataat
     5161 gcacagatta caggcggtat cggctacaac aaggcacgct attacggcac gctgatttcc
     5221 ccgaccgtat gtttgaatgc taccagcact tgtaccgata tgcaaaacac aaacgccagc
     5281 ggcaaagtaa cggcacgcaa ctatgactac aataccggta ctgcgcgcct gaccgaccaa
     5341 tatttccgca cattgagtat gaacctgacc gcacgcggcg aatttgaaac cggcccggta
     5401 acacataact ggagtatggc tttcgaccgc gttatccgcc agcggaaaac cataaacggc
     5461 agcgggaacg gcaacagcaa gatagaagtg aaggcaaacg aaaatattgc gaatcaattg
     5521 gcatcgttta cagccgacta tcccaattct tgggagaact ctgccaactt ggatgccaat
     5581 atcaaagtca acagcctggc tttgtccgac accttgggct tcgtggataa caaataccgc
     5641 ctgactttgg gcgaacgttt ccaaacagtc gaatataccg acaaaaaatc acagagcggc
     5701 gatgccaaac gtttcagccc gatgctgatg gcggcgtggg tgccgcaacc tgatttggtt
     5761 gtgtacggca actatatgga agacttggag cctgccgaca tcaaaaccga tgattccggc
     5821 gaaacgacaa tggcgaaacc gcgtgtcagc cgccagtttg aagtgggcgt gcgtaaaaac
     5881 tggggcaatt tcgtaaccac gttaaacgcg ttccaaatca aacgcccggg ctactggcgc
     5941 ggtcatacaa ctaccaagac aacaaaaggg gtaacaacaa cgctgaccta tggcaacaat
     6001 tccgatttcg cccgctataa ggcgcaaggc ggcgcggccg gcgacgagca aggtatggaa
     6061 cgcaatcgcg gtattgagtt caacacctac gcgaacttgc tcaacaatac tttgcgtccg
     6121 actttgggtc tgatgtatct gcaatcgacc gtgaaagagt atccaaattc acgcgatatg
     6181 ctggttaacg gcgtacaagt cgccaatccg cgcgtaattg ccaaagcagg tttggaatgg
     6241 gatacaccgt ttgccaaagg attgacttta aacggtaatg tttcgtattt cggcaagtct
     6301 taccaagaca cgcaaaaaca atacgccttc ccgtcctaca ccttgattga cgtaggcgcg
     6361 cgctacaaaa ccaagctcgg caaaaatact ttgaccgtca gcagctcggt ggaaaatctg
     6421 ttcaacaaaa actactggca ggtacagcgc ggccaatacg accgcagctt cgccgtcgtc
     6481 ggtctgccgc gtacttactg gctgaaggcg gaattggatt tctgatttct ccgcatctgg
     6541 aaaatgccgt ccgaacaggt ttcagacggc atttctttta aagagatatg gcgggatacg
     6601 cttaaataat gctattctag cgtctttgaa acctgcttgt ttgaagtgat gaaaaaacct
     6661 ttgatttcgg ttgcggcagc attgctcggc gttgctttgg gcacgcctta ttatttgggt
     6721 gtcaaagccg aagaaagctt gacgcagcag caaaaaatat tgcaggaagc gggcttcttg
     6781 accgtcgaat cgcaccaata tgagcgcggc tggtttacct ctacggaaac gacggtcatc
     6841 cgcttgaaac cggagttgct gaataatgcc cggaaatacc tgccggacaa cctgaaaaca
     6901 gtgttggaac agccggttac gctggtaaac catatcacgc acggcccttt cgccggcgga
     6961 ttcggcacgc aggcgtacat tgaaaccgag ttcaaatacg cgcctgaaac ggaaaaagtt
     7021 ctggaacgct tttttggaaa acaagtcccg gtttcccttg ccaataccgt ttattttaac
     7081 ggcagcggta aaatggaagt cagtgttccc gccttcgatt atgaagagct gtcgggcatc
     7141 aggctgcact gggaaggatt gacgggaaaa acggtttatc aaaaaggttt caaaagctac
     7201 cgcaacagct atgatgcgcc cttgttcaaa atcaagctgg cagacaaagg cgatgccgcg
     7261 tttgaaaaag cgcatttcga ttcggaaact tcagacggta tcaatccgct tgctttgggc
     7321 agcagcaatc tgaccttgga aaaattctcc ctagaatgga aagagggtgt cgattacaac
     7381 gtcaagttaa acgaactggt caatcttgtt accgatttgc agattggcgc gtttatcaat
     7441 cccaacggca gcatcgcacc ttccaaaatc gaagtcggca aactggcttt ttcaaccaag
     7501 accggggaat caggcgcgtt tatcgacagt gaagggcagt tccgttttgg cacactggtt
     7561 tacggcgatg aaaaatacgg cccgctggat attcatatcg ctgccgaaca cctcgatgct
     7621 tctgccttaa ccgtattgaa acgcaagttt gcacaaattt ctgccaaaaa aatgactgag
     7681 gaacaaatcc gcaatgattt gattgccgcc gtcaaaggcg aggcttctgg actgttcacc
     7741 aacaatcccg tattggacat taaaactttt cgtttcacgc tgccgtcagg aaaaatcgat
     7801 gtgggcggaa aaatcatgtt taaagatttg aagaaggaag atttgaacca attgggtttg
     7861 atgctgaaga aaaccgaggc agacatcaga atgagtattc ctcaaaaaat gttggaagac
     7921 ttggcggtca gtcaggcagg caatattttc agcgtcaatg ccgaagatga ggcggaaggc
     7981 agggcaagtc ttgacgacat caacgagacc ttgcgcctga tggtggacag tacggttcag
     8041 agtatggcaa gggaaaaata tctgactttg aacggcgacc agattgatac tgccatttct
     8101 ctgaaaaaca atcagttgaa attgaacggt aaaacgttgc aaaacgaacc ggagccggat
     8161 tttgatgaag gcggtatggt ttcagagccg cagcagtaat gggtcaaatg ccgtctgaaa
     8221 tgttcaggcg gcattttgtt gtattggaaa ctgccgtttt caaccattcc tttagataag
     8281 ccgtgtcgga ttc
//
LOCUS       DAWFHY010000086         8044 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.086, whole genome shotgun sequence.
ACCESSION   DAWFHY010000086 DAWFHY010000000
VERSION     DAWFHY010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8044
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.086"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            101..1033
                     /gene="pip"
                     /locus_tag="WGQ68_001739"
     CDS             101..1033
                     /gene="pip"
                     /locus_tag="WGQ68_001739"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEZ0715505.1"
                     /translation="MYEIKQPFHSGYLQVSEIHQIYWEESGNPDGVPVIFLHGGPGAG
                     ASPECRGFFNPDVFRIVIIDQRGCGRSRPYACAEDNTTWDLVADIEKVREMLGIGKWL
                     VFGGSWGSTLSLAYAQTHPERVKGLVLRGIFLCRPSETVWLNEAGGVSRIYPEQWQKF
                     VAPIAENRRNQLIEAYHGLLFHQDEEVCLSAAKAWADWESYLIRFEPEEVDEDAYASL
                     AIARLENHYFVNGGWLQGDRAILNNIGKIRHIPTIIVQGRYDLCTPMQSAWELSKAFP
                     EAELRVVQAGHCAFDPPLADALVRAVEDIFPRLL"
     gene            complement(1090..2286)
                     /gene="bamC"
                     /locus_tag="WGQ68_001740"
     CDS             complement(1090..2286)
                     /gene="bamC"
                     /locus_tag="WGQ68_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HEZ0715506.1"
                     /translation="MPSEAFRRHNATNTLISITQDDTMTHIKPVIAALALIGLAACSG
                     SKTEQPKLDYQSRSHRLIKLEVPPDLNNPDQGNLYRLPAGSGAVRASDLEKRRTPAVQ
                     QPADAEVLKSVKGVRLERDGSQRWLVVDGKSHAEIWPLLKAFWQENGFDIKSEEPAIG
                     QMETEWAENRAKIPQDSLRRLFDTVGLGGIYSTGERDKFIVRIEQGKNGVSDIFFAHK
                     AMKEVYGGKDKDTTMWQPSPSDPNLEAAFLTRFMQYLGVDGQQAENASAKNPTLPAAN
                     EMARIEGKSLIVFGDYGRNWRRTALALDRIGLTVVGQNTERHAFLVQKAPNESNAVTE
                     QKPGLFKRLLGKGKAEKPAEQPELIVYAEPVANGSRIVLLNKDGSAYAGKDASALLGK
                     LHSELR"
     gene            complement(2295..3170)
                     /locus_tag="WGQ68_001741"
     CDS             complement(2295..3170)
                     /locus_tag="WGQ68_001741"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEZ0715507.1"
                     /translation="MLQGSLVALITPMNQDGSIHYEQLRDLIDWHIENGTDGIVAVGT
                     TGESATLSVEEHTAVIEAVVKHVAKRVPVIAGTGANNTVEAIALSQAAEKAGADYTLS
                     VVPYYNKPSQEGMYRHFKAVAEAAAIPMILYNVPGRTVVSMNNETILRLAEIPNIVGV
                     KEASGNIGSNIELINRAPEGFFVLSGDDHTALPFMLCGGHGVITVAANAAPKLFADMC
                     RAALQGDIALARELNDRLIPIYDTMFCEPSPAAPKWAVSALGRCEPHVRLPLVPLTEG
                     GQAKVRAALKASGQL"
     gene            3477..4868
                     /locus_tag="WGQ68_001742"
     CDS             3477..4868
                     /locus_tag="WGQ68_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEZ0715508.1"
                     /translation="MSGHMGKGADAPDLVYGLEDRPPFGNALLSAVTHLLAIFVPMIT
                     PALIVGGALELPVEMTAYLVSMAMVASGVGTYLQVNRFGPVGSGMLSIQSVNFSFVTV
                     MIALGAGMKEGGLTKDAMISTLLGVSFVGAFLVCFSAWLLPYLKKVITPTVSGVVVML
                     IGLSLVHVGITDFGGGFGAKADGTFGSMENLGLASLVLLIVLVFNCMKNPLLRMSGIA
                     VGLIAGYIVALFLGKVDFSALQNLPLVTLPVPFKYGFAFDWHAFIVAGAIFLLSVFEA
                     VGDLTATAMVSDQPIEGEEYTKRLRGGVLADGLVSVIATALGSLPLTTFAQNNGVIQM
                     TGVASRHVGKYIAVILVLLGLFPVVGRAFTTIPSPVLGGAMVLMFGLIAIAGVRILVS
                     HGIRRREAVIAATSVGLGLGVAFEPEVFKNLPVLFQNSISAGGITAVLLNLVLPEDKT
                     EAAVKFDTDHLEH"
     gene            complement(4930..5682)
                     /gene="rlmB"
                     /locus_tag="WGQ68_001743"
     CDS             complement(4930..5682)
                     /gene="rlmB"
                     /locus_tag="WGQ68_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEZ0715509.1"
                     /translation="MANQRPIYGFHAVNARLWQNPKSIVELYIQEGKSDARTREVLEK
                     AANENIRVYFVDTTRLDAISKGARHQGVAGFIDASKNHVHLEDVLENLSEPPLLLILD
                     GITDPHNLGACLRTADAMGVHAVIAPKDKSAGLNATVSKVACGAAETVPYITVTNLAR
                     TLRELKEYGIWIIGTDMSGESDLYHCNLPDRAAWVMGNEGDGMRRLTREHCDMLVSIP
                     MFGTVESMNVSVSAGMVLSETRRQRVLKNEKV"
     gene            complement(5748..6236)
                     /locus_tag="WGQ68_001744"
     CDS             complement(5748..6236)
                     /locus_tag="WGQ68_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HEZ0715510.1"
                     /translation="MTVLTVILALALIAVGTAGIVYPALPGLALMFAGTWLLAYSGGY
                     QIYGAGVLWTVGLISLAGILADYVAGILGVKYTGAGRLAVRGALVGSIVGIFFSLPGL
                     ILGPFIGAAAGELIDRRNMLQAGKAGLGTLLGLIVGTAFKIGCAVSILFILLVKYIAY
                     LF"
     gene            complement(6241..6960)
                     /locus_tag="WGQ68_001745"
     CDS             complement(6241..6960)
                     /locus_tag="WGQ68_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEZ0715511.1"
                     /translation="MLTTPPLAPKTVAALHRLGIRTLEELRQNGSVKAFLLLKASGLT
                     LTKSTLWQLQSLLDDTLPQEMSQAHKARLLAELKNHPPVAAFPSQEEMEHFMREALRQ
                     AEQSAADGEIPVGAVIVSDGKIIASAHNTCIADCNVSRHAEINALAQAGREIQNYRLD
                     GCDIYITLEPCAMCASALIQARIRRVIYGAAEPKTGAAGSIVNLFADKRLNTHTAIRG
                     GILQEECRAVLSRFFQNKRKG"
     gene            complement(6964..7905)
                     /gene="miaA"
                     /locus_tag="WGQ68_001746"
     CDS             complement(6964..7905)
                     /gene="miaA"
                     /locus_tag="WGQ68_001746"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017629168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEZ0715512.1"
                     /translation="MPIPKAFALLGPTACGKTALALKIAETLPVEIISLDSALVYTGM
                     DIGTAKPSASERAFVPHHLIDIITPVQTYSAARFVEDCTRLIGEITARGKCPLIVGGT
                     MMYFRALTQGLNDLPEADACLRADLDEQKQMYGLDFLYRTLQKVDPETACRLKPNDSQ
                     RIERALEVYYLTGKPMSEHLGRQSPHTLPFDLHTAALIPENRARLHENIALRFHLMLE
                     QGFIGEVENLRRRYPGLTADSPAIRCVGYRQAWEYLDGKTDFPAFVEKGIAATRQLAK
                     RQLTWLRKTPLDCVADPFSDGTSCTRLIEAAKRFFGA"
BASE COUNT         1473 a         1874 c         2633 g         2064 t
ORIGIN      
        1 cgcgattttt cggcatttgc ctttcggggc ggcttgtgtc tcgtgcgtga tgttgcgtgt
       61 ggggatgttc ggattgtcag aagcaatata ggagaagatg atgtatgaaa taaaacagcc
      121 ttttcatagc ggatacttgc aggtgtctga aattcatcaa atttattggg aagagtcggg
      181 caatcccgac ggtgtgccgg tcattttttt acacggcggg ccgggcgcgg gggcttcgcc
      241 tgaatgtcgg ggttttttca atcccgatgt gttccgcatc gtcatcatcg accagcgcgg
      301 ttgcggacgt tcgcgcccgt atgcttgtgc ggaagacaat acgacttggg atttggtggc
      361 ggatattgaa aaagtccgtg aaatgctggg tatcgggaaa tggctggtgt tcggcggttc
      421 gtggggcagc actttgtcgc tggcttatgc ccaaacccat cctgaacggg taaagggatt
      481 ggtgttgcgc gggatatttt tgtgcagacc gtctgaaacg gtgtggctga acgaggcggg
      541 cggtgtgagc cggatttatc cggaacaatg gcaaaaattt gtcgcgccga ttgctgaaaa
      601 tcggcggaac cagctgattg aggcgtatca cggattgctg tttcatcaag atgaagaagt
      661 gtgcctgtct gccgcgaagg cttgggcgga ttgggaaagc tatctgatcc gtttcgagcc
      721 ggaggaagtg gatgaagatg cttatgcctc actggcaatc gcgcgtttgg aaaaccatta
      781 ttttgtcaac ggcggctggt tgcagggcga tagggcgatt ttgaacaata tcggcaaaat
      841 acggcatatc ccgaccatta tcgtgcaggg gcggtatgat ttgtgtacgc cgatgcagag
      901 tgcgtgggag ttgtcgaaag cctttcccga agcggaattg agggtggttc aggcagggca
      961 ttgtgcgttc gatccgcctt tggcggatgc gctggtgcgg gcggttgagg atatttttcc
     1021 tcggttgttg taaaaagttc cgcataaaaa agcagcttct gtttggaagc tgcttttgtt
     1081 ttgaacggtt taacgcagtt cggaatggag tttgcccaat aatgcggatg cgtctttgcc
     1141 ggcatatgcg ctgccgtctt tgttgagcag gacgatgcgc gagccgttgg cgacaggctc
     1201 ggcatagaca atcagttccg gctgttcggc aggtttctcc gctttgcctt tgcccagcag
     1261 gcgtttgaac aggccgggtt tttgttcggt aactgcattg ctttcgttcg gggctttttg
     1321 aaccaggaaa gcgtggcgtt cggtgttttg accgacgacg gtcagcccga tgcggtcgag
     1381 ggcgagcgcg gtgcgccgcc agtttctgcc gtagtcgcca aagacaatca ggcttttgcc
     1441 ttcgatacgc gccatttcgt tggcggcggg cagggtcggg ttttttgccg atgcgttttc
     1501 cgcctgctgt ccgtcaacgc ccaaatattg cataaagcgc gtcaggaaag cggcttcgag
     1561 gttgggatcg gacggggagg gctgccacat ggtcgtgtct ttgtctttgc cgccgtacac
     1621 ttctttcatg gctttgtggg cgaagaagat gtcggaaacg ccgtttttgc cctgttcgat
     1681 acggacgatg aatttgtcgc gctcgccggt ggagtagatg ccgcccaaac cgactgtgtc
     1741 gaataggcgg cgcaagctgt cttgggggat tttggcacgg ttttccgccc actcggtttc
     1801 catttgtccg atggcgggtt cttcggattt gatgtcgaag ccgttttcct gccaaaaggc
     1861 tttcaggagc ggccagattt cggcatgaga cttgccgtcg acaacgagcc agcgttggct
     1921 gccgtcgcgc tcgaggcgga cacctttgac gcttttcaat acttcggcat cggcaggctg
     1981 ttggacggcg ggtgtgcggc gtttttccaa atcgctggcg cggacggcgc ccgaaccggc
     2041 aggcaggcgg tagaggttgc cttggtcggg gttgttcaaa tcaggtggga cttcgagttt
     2101 gatcaggcgg tgcgaccggc tttggtagtc gagcttgggc tgttcggttt tgctgccgga
     2161 gcaggcggca agcccgatga gtgcgagcgc ggcaatgacg ggtttgatat gggtcatcgt
     2221 gtcatcctgt gtgatggata ttaaagtgtt tgttgcgtta tgccgtctga acgcttcaga
     2281 cggcatggct atatttaaag ttgtcctgag gctttcaggg cggcgcggac ttttgcctgt
     2341 ccgccttccg tcagcggaac gagcggcagg cggacgtgcg gttcgcatct gcccagggcg
     2401 gataccgccc atttcggtgc ggcggggctg ggttcgcaga acatggtgtc gtaaatcgga
     2461 atcagccggt cgttgagttc gcgtgcaagg gcgatatcgc cttgaagcgc ggcgcggcac
     2521 atatcggcaa agagcttggg cgcggcgttg gcggctacgg taatcacgcc gtgtccgccg
     2581 cacagcatga acggcagggc ggtatggtcg tcgccggaaa ggacgaagaa gccttcgggc
     2641 gcgcggttga tgagttcgat gttgctgccg atgttgccgc tggcttcttt cacgccgacg
     2701 atgttgggga tttcggcaag gcgcaggatg gtttcgttgt tcatgctgac gacggttctg
     2761 ccgggtacgt tgtagagtat catcggaatc gcggcggctt cggcaacggc tttgaaatgg
     2821 cggtacatgc cttcttgcga aggtttgttg taatagggga cgacggagag ggtgtagtcc
     2881 gccccggctt tttcggcggc ttgggaaagg gcgatggctt cgacggtgtt gtttgcccct
     2941 gtgccggcga tgacggggac gcgtttggca acgtgtttga cgacggcttc gatgacggcg
     3001 gtgtgttctt cgacggagag ggtggcggat tcgcctgtcg tgccgacggc aacgatgccg
     3061 tccgtgccgt tttcaatgtg ccagtcgatt aagtcgcgga gttgttcgta atggatgctg
     3121 ccgtcttgat tcatcggggt aatcagggca accaggctac cttgtaacat acagaacctt
     3181 ttatcagttg tggtgtaggg ggcggtaatg cttccgattg tagcctactt taccgcaggt
     3241 gtgaaatccg gcgggtccgg gatgagcggg gcggcttgac ggcggatgcg tcgggtttgt
     3301 atttttgtgt ttgaaatcag gttattgcgt tttgtaaacg atattccggc ggcgacggtt
     3361 cggaatctgt ttgccggacg ggcgcgtatg ccgtccgaaa tcgaaattga agcggtatgg
     3421 cggattcgct acaatccgcg ctttggtttt ttcaacattt gaagcaagga aacattatga
     3481 gcggtcatat gggaaaaggg gcggatgcgc ctgatttggt gtacggtttg gaggataggc
     3541 cgccgttcgg taatgcgctg ttgagcgcgg ttacccatct tttggcgatt ttcgtgccga
     3601 tgattacgcc cgcgctgatt gtgggcggcg cgctggaact gccggtggag atgacggcgt
     3661 atctcgtgtc gatggcgatg gttgcgtcgg gtgtcggcac ttatttgcag gtcaaccgct
     3721 tcgggccggt cggttcgggg atgctgtcca tccagtcggt gaatttctcg ttcgttaccg
     3781 tcatgattgc gctgggcgcg gggatgaaag agggcggttt gactaaggat gcgatgattt
     3841 cgacgctctt gggcgtatcg tttgtcggcg cgtttttggt gtgtttctcg gcgtggcttc
     3901 tgccgtattt gaaaaaagtg attacgccga cggtcagcgg cgtggtcgtg atgctcattg
     3961 gtttgagttt ggtacacgtc ggcattaccg atttcggcgg cggcttcggc gcgaaggcgg
     4021 acggcacgtt cggctcgatg gaaaacttgg ggctggcttc gctggtgttg ctgattgtgt
     4081 tggtgttcaa ctgcatgaaa aacccgctgt tgcgcatgag cggcattgcg gtcgggctga
     4141 ttgccggcta tatcgtcgcg ctgtttttgg gcaaggtgga tttttccgcg ctgcaaaacc
     4201 tgccgctggt tacgctgccc gtaccgttta aatacggttt tgctttcgac tggcacgcgt
     4261 ttattgtggc gggcgcgatt ttcttgttga gcgtgtttga ggcggtcggc gatttaaccg
     4321 cgacggcaat ggtgtccgac cagccgattg aaggcgagga atacaccaaa cgcctgcgcg
     4381 gcggcgtgtt ggctgacggc ttggtgtcgg tgattgcgac ggctttgggt tcgctgccgc
     4441 tgacgacgtt tgcgcaaaac aacggcgtga ttcagatgac cggcgtggct tcgcgccatg
     4501 tgggcaaata tattgccgtg attttggtgc tgttgggtct gttccccgtt gtcggacgcg
     4561 cgtttacgac gattccgagt ccggtgttgg gcggcgcgat ggttttgatg ttcggcttga
     4621 ttgcgattgc gggcgtgcgg attttggtca gtcacggcat ccgcaggcgc gaagcggtaa
     4681 ttgcggcaac gtcggtcggt ttgggcttgg gtgtcgcgtt tgagccggaa gtgtttaaaa
     4741 acctgcccgt cttgttccaa aactctattt ccgccggcgg cattacggca gtcttgctga
     4801 atttggtctt gcccgaagat aaaaccgagg cggcggtcaa gtttgatacc gaccacttgg
     4861 aacactgatt ttgaaaatga atgccgtctg aaacagaatc cctgtttcag acggcattgt
     4921 ttttgaggct tatacttttt cgttttttaa tacgcgttgt cggcgtgttt cacttaatac
     4981 cattccggca gacacggaga cgttcatgct ttcgactgtg ccgaacatgg gtatagacac
     5041 cagcatgtcg caatgttcgc gcgtgaggcg gcgcataccg tcgccttcgt tgcccattac
     5101 ccacgccgct ctgtcgggca gattgcaatg gtaaaggtcg gactcgccgc tcatatcggt
     5161 gccgataatc caaatgccgt attctttcaa ttcgcgcagg gtgcgggcga ggttggttac
     5221 ggtgatatag gggacggttt ccgccgcacc gcaggcgact ttgctgacgg tggcgttcag
     5281 ccccgcgctt ttgtctttcg gtgcgatgac ggcgtgtacg cccattgcgt cggcggtacg
     5341 caggcatgcg ccgaggttgt gcgggtcggt gatgccgtcg agtatcagca gcagcggcgg
     5401 ttcgcttaag ttttccaata cgtcttcgag atggacgtgg tttttggagg catcgataaa
     5461 tccggccaca ccctgatggc gcgcgccttt gctgatggca tcaagacggg tggtgtcaac
     5521 aaaatatacg cggatgtttt cgtttgccgc cttttccaac acttcgcgcg tgcgtgcgtc
     5581 tgatttgcct tcttggatgt agagttcgac gatggatttg gggttttgcc acaaacgggc
     5641 attgacggcg tggaagccgt agatgggtct ttggtttgcc atgatggtgc tttgtcggaa
     5701 atttcagacg gcattatagc aatttatccg tatgccgtct gaaggggtta aaacaggtag
     5761 gcgatgtatt tcaccaacag gataaacaag atggatacgg cgcagccgat tttgaacgcc
     5821 gtaccgacga taagccccaa cagcgtaccc aagcccgctt tacctgcctg aagcatattg
     5881 cgtctgtcga tgagttcgcc tgccgccgcg ccgataaagg ggccgagtat tagtccggga
     5941 agggagaaaa atatgccgac gatgctgccg accaatgcgc cgcgaacggc gagcctgccc
     6001 gctccggtat attttacccc caatatgcct gccacatagt ccgccagtat gccggcaagg
     6061 ctgatgagtc cgaccgtcca caaaacacct gcgccgtaga tttggtagcc gccggagtag
     6121 gcaagcagcc atgttccggc aaacatcaat gccaatccgg gcagggcggg gtaaacgatg
     6181 cccgccgtgc cgacggctat cagggcgagg gcgaggatga cggtcagtac ggtcataggt
     6241 tcaacctttt cttttgttct gaaaaaaacg gcttaatacg gcgcggcatt cttcttgcag
     6301 gattccgccc cgtatggcgg tgtgcgtatt gaggcgtttg tcggcaaaca ggttgacaat
     6361 gctgcctgcc gcgccggttt tgggttctgc cgcaccatat atcacacgcc tgattcgtgc
     6421 ctgtatcagt gcggacgcgc acatggcgca gggttcgagg gtgatatata tgtcgcatcc
     6481 gtcaaggcgg tagttttgta tttctctgcc tgcctgtgcc aaggcgttga tttcggcgtg
     6541 tcggctgaca ttgcagtcgg caatgcaggt gttgtgtgcc gatgcgatga ttttgccgtc
     6601 tgaaacaatg actgccccga cgggtatttc gccgtcggcg gcggattgtt ctgcttggcg
     6661 cagtgcttcg cgcatgaagt gttccatttc ttcctgcgat gggaaggcgg cgacgggcgg
     6721 atggtttttc aattcggcaa gaaggcgggc tttatgcgct tgggacattt cttgcggcag
     6781 cgtgtcgtcc agcagcgact gaagctgcca cagtgtgctt ttcgtgaggg tcaaacccga
     6841 tgctttgagc agcagaaagg ctttgaccga accgttttgc cgcagttctt cgagcgtacg
     6901 gataccgagc ctgtgcaggg cggcgacggt tttgggggcg agcggcggtg tggtcagcat
     6961 ggtttatgcg ccgaaaaacc gttttgccgc ctcaatcagg cgtgtgcatg aagtgccgtc
     7021 tgaaaacggg tcggcaacgc agtctaaagg tgttttgcgc aaccaagtca gttggcgttt
     7081 ggcaagttgg cgggtggcgg caatgccttt ctcgacaaat gccgggaaat cggtttttcc
     7141 gtctagatat tcccatgcct gacggtagcc gacgcagcgg atggcggggg agtcggcggt
     7201 caggccggga tagcggcggc gcaggttttc tacttcgccg ataaagccct gttcaagcat
     7261 caggtggaaa cgcagggcga tgttttcatg caggcgggcg cggttttcgg gaatcagggc
     7321 ggcggtatgc aaatcgaagg ggagcgtatg ggggctttgt ctgccgaggt gttcgctcat
     7381 cggtttgccg gtcagataat aaacttccaa agcgcgttcg atacgctggc tgtcgttcgg
     7441 tttcagacgg catgcggttt cagggtcgac tttttgcagg gtgcggtaga ggaaatccaa
     7501 gccgtacatc tgtttttgtt cgtccaagtc ggcacgcagg caggcatcgg cctcgggcaa
     7561 atcgttcaaa ccttgggtca gggcgcggaa atacatcatt gtgccgccga caatcagcgg
     7621 acatttccca cgcgccgtga tttcgccgat cagacgcgtg cagtcttcga caaaacgggc
     7681 ggcactgtat gtctgaacag gcgtaatgat gtcgataagg tggtgcggga caaaggcgcg
     7741 ttcggaggcg gacggttttg ccgtgccgat gtccataccg gtatagacta gcgcggaatc
     7801 gaggctgatg atttcgacag gcagggtttc ggcaattttg agggcgagcg cggttttgcc
     7861 gcaggcggtc gggcctaaga gggcaaaggc tttgggaatc ggcatgatgt ttcagggttg
     7921 cgaaaatgcg gattatagcg gaaagcgtgc cgacgttata ttttggtttg cggaagcacg
     7981 ccgacggcaa gggggcgtgt ttaccgtatg cctttatata gtggattaac aaaaaccagt
     8041 acgg
//
LOCUS       DAWFHY010000087         8038 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.087, whole genome shotgun sequence.
ACCESSION   DAWFHY010000087 DAWFHY010000000
VERSION     DAWFHY010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 8038)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8038)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8038
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.087"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            223..321
                     /locus_tag="WGQ68_001747"
     CDS             223..321
                     /locus_tag="WGQ68_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014575118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eamA-like transporter family protein"
                     /protein_id="HEZ0715513.1"
                     /translation="MAVVFLDERPNTQEWIGLGLVTAGVLTLALKR"
     gene            complement(392..1777)
                     /locus_tag="WGQ68_001748"
     CDS             complement(392..1777)
                     /locus_tag="WGQ68_001748"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HEZ0715514.1"
                     /translation="MVSIFDIFKIGIGPSSSHTVGPMKAAAAFATGLDAQAVRIVIDI
                     YGSLALTGYGHGTFDALMLGLEGSLPHDIPLAGIPERLERIRTQHILRLNGQEIRFIP
                     DRDLNILGNQVLPKHPNGLRFTAYASDGTVLNEQVYYSVGGGFVVTEEDFDRQAETEK
                     AVPYPYTSCAELLARCRLNRLDISEVVLANEAALAGCGEAEIRRRAAAVAEVMEGCIK
                     RGLGADGELPGGLNVRRRAPQLAAKLKVLRETEIVNTQLWPMVYAMAVNEENAAGGRV
                     VTAPTNGAAGIIPAVLHYFRKFNPHATQERVENFLLTAGAIGILYKTNASISGADVGC
                     QGEVGVACSMAAGAYAEVIGGTPKQVENAAEMAMEHHLGLTCDPVGGLVQIPCIERNG
                     IAAEKALKLGTLALLEDGTDKKVSLDEVIRTMLQTGRDMKATYKETSLAGLAATLRKK
                     AVPVSVRVVEC"
     gene            complement(1876..4266)
                     /gene="gyrB"
                     /locus_tag="WGQ68_001749"
     CDS             complement(1876..4266)
                     /gene="gyrB"
                     /locus_tag="WGQ68_001749"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009117835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEZ0715515.1"
                     /translation="MTEQKHEEYGADSIQVLEGLEAVRKRPGMYIGDTQDGSGLHHMV
                     FEVLDNAIDEALAGHCDKITVTIHADHSVSVADNGRGMPTGIHPKEGRSAAEVIMTVL
                     HAGGKFDNNSYKISGGLHGVGVSVVNALSDWVTLTIYRDGKEHFVRFVRGETEEPLKV
                     VGDSDKKGTTVRFLASTETFGNVEYSFDILAKRIRELSFLNNGVDIELTDERDGKHES
                     FALSGGVAGFVQYMNRKKTPLHEKIFYAFGEKDGMSVECAMQWNDSYQESVQCFTNNI
                     PQRDGGTHLTALRQVMTRTINNYIEANEVAKKAKVETAGDDMREGLTCVLSVKLPDPK
                     FSSQTKDKLVSGEIGPVVNEVISQALTDFLEENPAEAKIITGKIVDAARAREAARKAR
                     EITRRKGVMDGLGLPGKLADCQEKDPALSELYLVEGDSAGGSAMQGRDRKFQAILPLK
                     GKILNVEKARFEKMLASQEVAALITALGAGIGKEEFNAEKLRYHRIIIMTDADVDGAH
                     IRTLLLTFFYRQMPELVERGYIYIAQPPLYKAKYGKQERYLKDELEKDQWLLGLALEK
                     AKIISDGRTIEGAELADTAKQFLLAKTVIEQESRFVDELVLRAMLHASPIDLTSSENA
                     DKAVAELSGLLDEKEVALERIEGHEGHRFIKITRKLHGNVMVSYIEPKFLNSKAYQTL
                     TQTAAALKGMVGEGAKLYKGENGYDADSFETALDILMSVAQKGMSIQRYKGLGEMNPE
                     QLWETTMDPAVRRLLKVRIEDAIAADEVFVTLMGDEVEPRRAFIENNALIAQNIDA"
     gene            4536..5408
                     /locus_tag="WGQ68_001750"
     CDS             4536..5408
                     /locus_tag="WGQ68_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEZ0715516.1"
                     /translation="MEIWNMLDTWLGAVPIRAEAVESVAAVAALLLARALLLNIHFKR
                     HPDFGIESKRRFLVASRNITLLLVLFSLAFIWSAQIQTLALSMFAVAAAVVVATKELI
                     MCLSGSILRSATQQYSVGDYIEINGLRGRVVDINLLNTLMMQVGPNPLVGQLAGTTVS
                     FPNSLLLSHPVRRDNILGDYVIHTVEIPVPIHLDSDEAVCRLKAVLEPLCAPYIPAIQ
                     RHLENVQAEKLFITPAARPRVTRVPYDDKAYRIIVRFASPVSKRLEIQQAVMDEFLRV
                     QYRLLNYPAGSETL"
     gene            5496..7532
                     /locus_tag="WGQ68_001751"
     CDS             5496..7532
                     /locus_tag="WGQ68_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019270417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HEZ0715517.1"
                     /translation="MTDNALLHLGEEPRFDQIKTEDIKPALQTAIAEAREQIAAIKAQ
                     THTGWANTVEPLTGITERVGRIWGVVSHLNSVADTPELRAVYNELMPEITVFFTEIGQ
                     DIELYNRFKTIKNSPEFDTLSPAQKTKLNHDLRDFVLSGAELPPEQQAELAKLQTEGA
                     QLSAKFSQNVLDATDAFGIYFDDAAPLAGIPEDALAMFAAAAQSEGKTGYKIGLQIPH
                     YLAVIQYADNRELREQIYRAYVTRASELLDGGKFDNTANIDRTLANALQTAKLLGFKN
                     YAELSLATKMADTPEQVLNFLHDLARRAKPYAEKDLAEVKAFARESLNLADLQPWDLG
                     YASEKLREAKYAFSETEVKKYFPVGKVLNGLFAQIKKLYGIGFTEKTVPVWHKDVRYF
                     ELQQNGETIGGVYMDLYAREGKRGGAWMNDYKGRRRFSDGTLQLPTAYLVCNFAPPVG
                     GREARLSHDEILTLFHETGHGLHHLLTQVDELGVSGINGVEWDAVELPSQFMENFVWE
                     YNVLAQMSTHEETGEPLPKELFDKMLAAKNFQRGMFLVRQMEFALFDMMIYSEDDEGR
                     LKNWQQVLDSVRKEVAVIQPPEYNRFALSFGHIFAGGYSAGYYSYAWAEVLSADAYAA
                     FEESDDVAATGKRFWQEILAVGGSRSAAESFKAFRGREPSIDALLRHSGFDNAA"
BASE COUNT         1729 a         2256 c         2200 g         1853 t
ORIGIN      
        1 gcatatttta gaaaagacct tttatagtgg attaacaaaa accagtacag cgttgcctcg
       61 ccttagctca aagagaacga ttctctaagg tactgaagca ccaagtgaat cggttccgta
      121 ctatttgtac tgtctgcggc ttcgtcgcct tgtcctgatt tttgttaatc cactataaaa
      181 attcttgccg gatgctgcaa acaacgccgg tttgcattcc tgatggcggt ggttttctta
      241 gacgaacgcc cgaacacgca ggaatggata ggcttggggc tggttacggc gggcgtgttg
      301 acgctggcac tgaaacggta aagccgcaag aaataaatga aatgccgtct aaaaaactgt
      361 tttcagacgg cattttcgtt tctgtccatc ctcagcactc gaccacgcgc acggatacgg
      421 ggacggcttt tttccggagc gtggcggcga gtccggcaag agaggtttct ttgtaggtcg
      481 ccttcatatc ccggccggtt tgcagcatgg ttcggatgac ttcgtcgagc gagacttttt
      541 tgtccgtgcc gtcttccaaa agcgcgagcg tgccgagttt gagggctttt tcggcggcga
      601 tgccgttgcg ctcgatgcag gggatttgca ccagtccgcc gacggggtcg caagtcagcc
      661 ccaaatggtg ttccatcgcc atttcggcgg cgttttccac ttgtttgggc gtgccgccga
      721 tgacttcggc gtatgcgccc gccgccatcg aacacgctac gccgacttcg ccctgacagc
      781 cgacatccgc accggaaatg gaggcgttgg tcttgtagag gatgccgatt gcgcctgcgg
      841 tgagcaggaa gttttcgacg cgttcctgtg tggcgtgcgg attgaacttg cggaaatagt
      901 gcaatacggc gggaatgatg cctgccgcgc cgttggtcgg tgcggtaacg acgcgtccgc
      961 cggcggcgtt ttcttcgttg accgccatgg cgtacaccat cggccagagc tgggtgttga
     1021 cgatttcggt ttcgcgcagg actttgagct tggcggcaag ctgcggggcg cggcggcgga
     1081 cgttcaatcc gccgggcagt tcgccgtccg cacccaagcc gcgtttgatg cagccttcca
     1141 taacctcggc aacggcagcg gcgcggcggc ggatttcggc ttcgccgcat ccggcaagcg
     1201 cggcttcgtt tgccaacacg acttcggaga tgtcgagccg gttcagacgg catcgggcaa
     1261 gcagttcggc gcaactggta tagggatagg gaacggcttt ttccgtttcc gcctgccggt
     1321 caaaatcttc ttcggtaacg acaaagccgc cgccgaccga ataataaacc tgttcattca
     1381 ataccgtgcc gtctgaagca taggcggtaa aacgcaggcc gttggggtgt ttgggcagca
     1441 cttgattgcc gagtatgttc aggtcgcggt cggggatgaa gcggatttct tgcccgttga
     1501 gccggaggat gtgctgcgtg cggatgcgtt cgaggcgttc gggaatgccg gcaagcggga
     1561 tgtcgtgcgg caggctgcct tccaaaccga gcatcagcgc gtcaaatgta ccgtgtccgt
     1621 atccggtcag tgcgagcgag ccgtaaatgt cgatgacgat gcgaacagcc tgtgcatcca
     1681 aacctgtcgc aaaggcggcg gctgccttca tcgggccgac cgtatgcgaa ctggaaggcc
     1741 cgataccgat tttgaaaata tcaaaaatgc tgaccatggt tttgctccga aaggcggttt
     1801 cagacggtat ggcttccgtt tgacaaacca aaaggagacg cggcacgatg cccgtctcct
     1861 tttttaaaac ggcacttatg cgtcgatatt ttgggcaatc agcgcgttgt tttcgataaa
     1921 ggcacggcgc ggctcgacct cgtcgcccat cagcgtaacg aacacttcgt cggcggcaat
     1981 ggcatcttcg atgcgcactt tcaacaggcg gcgcacggcg ggatccatcg tggtttccca
     2041 cagctgctcg gggttcatct cgcccaagcc tttgtatcgt tggatggaca tacctttttg
     2101 ggcaacgctc atcaagatgt ccaaagcggt ttcaaagctg tccgcgtcgt acccgttttc
     2161 gcctttgtaa agcttggcac cctcgccgac catgcctttg agcgcggcgg cggtttgggt
     2221 gagggtttgg taggctttgc tgttgaggaa cttgggttcg atgtagctga ccatgacgtt
     2281 gccgtgcagc ttgcgcgtga ttttgatgaa ccggtgtcct tcatgacctt cgatgcgttc
     2341 gagggcgact tctttttcgt caagcagacc ggaaagttcg gcaacggctt tatcggcgtt
     2401 ttcagacgac gtcaaatcaa tgggcgacgc gtgtagcatg gcgcgcagga cgagttcgtc
     2461 tacgaagcgg ctttcctgtt cgatgacggt ttttgccaac aggaattgtt tggcggtgtc
     2521 ggcaagttct gcgccttcga tggtgcggcc gtctgaaatg attttggctt tttccaaggc
     2581 aagaccgagc agccattggt ctttttccaa ttcgtctttg agatatcgtt cttgtttgcc
     2641 gtatttggct ttgtataaag gcggctgggc aatgtagatg tagccgcgtt cgaccagctc
     2701 gggcatttgg cggtagaaga aggtcaggag cagggtgcgg atgtgcgcgc cgtccacgtc
     2761 ggcatcggtc atgatgatga tgcggtggta acgcagtttt tcggcattga attcttcttt
     2821 gccgatgccc gcgcccaacg cggtaatcaa agcggctact tcctgactgg cgagcatttt
     2881 ttcaaagcgc gctttttcga cgttcaaaat cttgccctta agcggcaaaa tcgcttggaa
     2941 tttgcggtcg cgcccctgca tggcggaacc gcctgcagaa tcgccctcga ccagataaag
     3001 ttctgacaat gcggggtctt tttcttggca gtcggcgagt ttgccgggta gccccaagcc
     3061 gtccatcacg cctttgcggc gggtgatttc gcgtgctttg cgggcggctt cgcgcgcgcg
     3121 ggcggcatcg acgattttgc cggtgatgat tttggcttcg gcagggtttt cttcgaggaa
     3181 gtcggtcagg gcttggctga tgacttcgtt gacgacgggg ccgatttcgc cggaaaccag
     3241 tttgtctttg gtttgcgatg agaatttcgg atcgggcagt ttgacggaca aaacgcaggt
     3301 cagaccttca cgcatatcgt cgccggcggt ttccactttg gcttttttag ccacttcgtt
     3361 ggcttcgata tagttgttga tggtgcgggt catcacttgg cgcaacgcgg tcaagtgggt
     3421 gccgccgtcg cgttgcggaa tgttgttggt aaaacactgc acgctttctt ggtagctgtc
     3481 attccactgc atcgcgcatt cgacgctcat gccgtctttc tcgccgaacg cgtagaagat
     3541 tttttcgtgc aagggcgttt ttttgcggtt catgtattgc acgaatccag ccacgccgcc
     3601 ggaaagggcg aagctttcgt gtttgccgtc gcgctcgtcg gtcaattcga tgtccacgcc
     3661 gttgttcagg aacgaaagtt cgcggatgcg tttggcaagg atgtcgaagc tgtattcgac
     3721 gttgccgaag gtttccgtac tggcgaggaa gcgcacggtc gtgccttttt tatcggaatc
     3781 gccgaccact ttcaacggct cttcggtttc gccgcgcacg aagcggacga agtgttcttt
     3841 gccgtcgcgg tagatggtca gcgtaaccca gtcggacagc gcgttgacga cggacacgcc
     3901 cacgccgtgc aggccgccgg agattttgta gctgttgttg tcgaatttac cgcccgcgtg
     3961 caatacggtc atgatgactt cggcggcgga gcgtccttct ttcgggtgga tgccggtggg
     4021 cataccgcgc ccgttgtcgg cgacgctgac ggaatggtcg gcgtgtatcg ttaccgtgat
     4081 tttgtcgcaa tgtccggcga gtgcttcgtc aatggcgttg tccaatactt cgaacaccat
     4141 gtggtgcaga ccgctgccgt cctgcgtgtc gccgatgtac atgccggggc gtttgcgtac
     4201 cgcttccaag ccttcgagca cctgaatgct gtcggcgccg tattcttcgt gtttttgttc
     4261 agtcatattt tttgccggat tttgaaaaga ttttgcgatg ccgccaaaac aagtccgcac
     4321 cttgtagaaa aagcgggcgg gacgacatat ataattgtgt attatagccg attttgccgc
     4381 ctaattcagc gttatccgca tcagtgtgcc gccgggaaaa gatgaaacgg tacgtttgcc
     4441 tccggcatca ggtcggggat tgtcccgtaa agtggcaaaa gcgttttttt gccactaaaa
     4501 tctacaccct atacttttcg gacaggggcg cggaaatgga aatatggaat atgttggaca
     4561 cttggctcgg tgccgtcccg atacgtgcgg aggcggtcga atccgtggcg gcggttgcgg
     4621 ctttgctgct ggcgcgcgcc cttctgttga atatccactt caaacggcat ccggatttcg
     4681 gcatcgaaag caagcggcgg tttttggttg ccagccgcaa tataacgctg cttttggtgc
     4741 tgttttcgct ggcatttatc tggtcggcgc aaatccaaac gctggctttg tcgatgtttg
     4801 cggtggcggc ggcggtcgtc gtggcgacga aggaactgat tatgtgtctg tcgggcagta
     4861 ttttaaggtc tgccacccag caatactcgg tcggcgacta tatcgaaatc aacggcctgc
     4921 gcgggcgcgt ggtcgacatc aacctgttga acacgctgat gatgcaggtc ggtccgaacc
     4981 ccttggtcgg acagcttgcg ggaaccaccg tttctttccc caacagcctg ttgttgagcc
     5041 accccgtgcg ccgcgacaat attttgggcg actatgtcat ccatacggtc gaaatccccg
     5101 ttcccatcca tttggattcg gatgaagccg tatgccgtct gaaagccgta ctcgagccct
     5161 tgtgcgcgcc ctacatcccc gccatccaac ggcatttgga aaacgtgcag gcggaaaaac
     5221 tgtttatcac gcccgccgcc agaccgcgcg ttacccgcgt gccgtacgat gacaaggcat
     5281 accgcatcat cgtccgcttc gcctcccccg tttcaaagcg gctggaaatc caacaggcgg
     5341 ttatggacga atttttgcgc gtacaatacc gcctgttaaa ttaccccgcc ggctccgaaa
     5401 cactttaact ttccccgacc gaccccattt ccggcttcag acggcatatt gccgatatgc
     5461 tgtctgaagc acaacacgca aaggaaaccc atcttatgac tgacaacgca ctgctccatt
     5521 tgggcgaaga accccgtttt gatcaaatca aaaccgaaga catcaaaccc gccctgcaaa
     5581 ccgccatcgc tgaagcgcgc gaacaaatcg ccgccatcaa agcccaaacg cacaccggct
     5641 gggcaaacac tgtcgaaccc ctgaccggca tcaccgaacg cgtcggcagg atttggggcg
     5701 tggtgtcgca cctcaactcc gtcgccgaca cgcccgaact gcgcgccgtc tataacgaac
     5761 tgatgcccga aatcaccgtc ttcttcaccg aaatcggaca agacatcgag ctgtacaacc
     5821 gcttcaaaac catcaaaaac tcccccgagt tcgacaccct ctcccccgcg caaaaaacca
     5881 aactcaacca cgatctgcgc gatttcgtcc tcagcggcgc ggaactgccg cccgaacagc
     5941 aggcagaact ggcaaaactg caaaccgaag gcgcgcaact ttccgccaaa ttctcccaaa
     6001 acgtcctaga cgcgaccgac gcgttcggca tttactttga cgatgccgca ccgcttgccg
     6061 gcattcccga agacgcgctc gccatgtttg ccgctgccgc gcaaagcgaa ggcaaaacag
     6121 gctacaaaat cggtttgcag attccgcact acctcgccgt catccaatac gccgacaacc
     6181 gcgaactgcg cgaacaaatc taccgcgcct acgttacccg cgccagcgaa cttttagacg
     6241 gcggcaaatt cgacaacacc gccaacatcg accgcacgct cgcaaacgcc ctgcaaaccg
     6301 ccaaactgct cggcttcaaa aactacgccg aattgtcgct ggcaaccaaa atggcggaca
     6361 cccccgaaca agttttaaac ttcctgcacg acctcgcccg ccgcgccaaa ccctacgccg
     6421 aaaaagacct cgccgaagtc aaagccttcg cccgcgaaag cctgaacctc gccgatttgc
     6481 aaccgtggga cttgggctac gccagcgaaa aactgcgcga agccaaatac gcgttcagcg
     6541 aaaccgaagt caaaaaatac ttccccgtcg gcaaagtatt aaacggactg ttcgcccaaa
     6601 tcaaaaaact ctacggcatc ggatttaccg aaaaaaccgt ccccgtctgg cacaaagacg
     6661 tgcgctattt tgaattgcaa caaaacggcg aaaccatagg cggcgtctat atggacctct
     6721 acgcccgcga aggcaaacgc ggcggcgcgt ggatgaacga ctacaaaggt cgccgccgtt
     6781 tttcagacgg cacgctgcaa ctgcccaccg cctacctcgt ctgcaacttc gccccacccg
     6841 tcggcggcag ggaagcccgc ctgagccatg acgaaatcct caccctcttc cacgaaaccg
     6901 gccacggcct gcaccacctg cttacccaag tggacgaact gggcgtgtcc ggtatcaacg
     6961 gcgtagaatg ggacgcggtc gaactgccca gccagtttat ggaaaatttc gtttgggaat
     7021 acaatgtatt ggcgcaaatg tccacccacg aagaaaccgg cgagcccctg ccgaaagaac
     7081 tcttcgacaa aatgctcgcc gccaaaaact tccaacgcgg aatgttcctc gtccgccaga
     7141 tggagttcgc cctcttcgat atgatgattt acagcgaaga cgacgaaggc cgtctgaaaa
     7201 actggcaaca ggttttagac agcgtgcgca aagaagtcgc cgtcatccaa ccgcccgaat
     7261 acaaccgctt cgccttgagc ttcggccaca tcttcgcagg cggctattcc gcaggctatt
     7321 acagctacgc gtgggcggaa gtattgagcg cggacgcata cgccgccttt gaagaaagcg
     7381 acgatgtcgc cgccacaggc aaacgctttt ggcaggaaat cctcgccgtc ggcggatcgc
     7441 gcagcgcggc agaatccttc aaagccttcc gcggccgcga accgagcata gacgcactct
     7501 tgcgccacag cggtttcgac aacgcggctt gatcgatgtg aacaaaaaat gccgtctgaa
     7561 acaatcaaac agtgtttcag acggcatcag catttgcgcg gtttcgccat tttcacaaaa
     7621 acagtaagcc aaaatcccgt cattcccgcg taggcgggaa tccagaccgg tcggcacgga
     7681 aacttatcgg ataaaacggt ttctttagat tttacgttct agattcccgc tttcgcggga
     7741 atgacgcgga aaagttgctg tgatttcgga taaattttcg caacgtttaa tttccgtttt
     7801 acctgataaa tgccctcaat ctcaaatccc gtcattcccc aaaaacaaaa aaaatcaaaa
     7861 acagaaatcc cgtcattccc gcgcaggcgg gaatccaggt ctgtcggtgc ggaaacttat
     7921 cggataaaac ggtttcttta gattttacgt tctagattcc cgctttcgcg ggaatgacgg
     7981 aatatttttg aatttgataa aaatgccgtc tgaaacggtc aaacaacgct tcagacgg
//
LOCUS       DAWFHY010000088         7995 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.088, whole genome shotgun sequence.
ACCESSION   DAWFHY010000088 DAWFHY010000000
VERSION     DAWFHY010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7995
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.088"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..2261)
                     /gene="clpA"
                     /locus_tag="WGQ68_001752"
     CDS             complement(<1..2261)
                     /gene="clpA"
                     /locus_tag="WGQ68_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HEZ0715518.1"
                     /translation="MLAPELEQILQQLYREARKAHYEFISLEHLLLVLIEEDASVPNV
                     LKLCGADLKVVSEQLAASVAENTPLIPEHLLDTVETRPTLGFQRVMQRAMVHTQSAGK
                     AAVEPLDVLVALMSETDSHTVYFLKLQSVTRFEVLRCIAHGSPDDEDGNDSDGLGREG
                     EEAEQKTGSLSDYTVNLNAEVKAGRIDPLIGRKHEMERLVQILCRRRKNNPLLVGEAG
                     VGKTALAEGLAHQIVNSDIPDALKDTEVYALDMGSLLAGTKYRGDFEARVKSVLKQLE
                     KIPHAILFIDEIHTIIGAGSTSGGTMDASNLLKPALAKGSLRCIGATTYDEYRTIFDK
                     DHALSRRFQKIDVVEPTVSETVQILRGLKPMFEAFHQVRYTQGALEAAAELSARYINE
                     RFLPDKAIDVMDEAGAAQRILPKSKQKKVIGKAQIETVIAKVSRIPEKTVSHDDKQVL
                     QFLGRDLKNMVYGQENAIDALVAAVKMSRSGLALPDKPIGSFLFSGPTGVGKTEAAKQ
                     LAYSLGVPLQRFDMSEYMERHAVSRLIGAPPGYVGFEQGGLLTEAVNKQPHCVLLLDE
                     IEKAHPDIFNVLLQVMDAGKLTDNNGKSADFRNVILIMTTNAGAESLSRPSLGFTAKR
                     ERGDEMQAINKLFTPEFRNRLDAIIPFAPLSEPIIVKVVDKFLLQLEHQLLDKKVEAE
                     FTSALHKYLAEKGFDPQMGARPMHRLIQEKIRKPLADELLFGKLADGGFVRIDWDTAK
                     EEAVLKFKKSKVK"
     gene            complement(2263..2565)
                     /gene="clpS"
                     /locus_tag="WGQ68_001753"
     CDS             complement(2263..2565)
                     /gene="clpS"
                     /locus_tag="WGQ68_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEZ0715519.1"
                     /translation="MTAQHQSDTLLHRLNTLPPKRYGVFLLNDDYTTMEFVVEILTEI
                     FMLGQEQAVAVMLLVHHEGKGLCGTYTRDIAQTKQQQVMQRAKAEGHPLQCIVEEI"
     gene            2808..3011
                     /locus_tag="WGQ68_001754"
     CDS             2808..3011
                     /locus_tag="WGQ68_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEZ0715520.1"
                     /translation="MATGIVKWFNDAKGFGFITPDEGGEDLFAHFSAINMEGFKTLKE
                     GQRVSFDVTTGPKGKQAANIQAA"
     gene            complement(3308..4639)
                     /gene="pmbA"
                     /locus_tag="WGQ68_001755"
     CDS             complement(3308..4639)
                     /gene="pmbA"
                     /locus_tag="WGQ68_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HEZ0715521.1"
                     /translation="MLFNHTASELLDLCRRTLDLAKAAGATAAEADFSESIGQSVSVR
                     LGEIEQIEFQQDKSLDITVYVGKRKGRAGTADFSEQALRDTVRAAADIARHTAEDGCA
                     GLADPELMAQHIGDPDLYHEWDLDTEAAVGFAKQCEQAALNEDERIENSEGAAVQTGH
                     YQYVYGNTHGFAAHRQGTHHSISCSVVAADENGMQRDYWYDSACRHPDMDSPETIGQT
                     AARRTLRRLGSRSIPTGSYPVLFDTTVSGGLIGHLVGALSGGALYRQSSFLIDSIGKK
                     VLPDFLNLREEPHIPRSFRSSYFDAEGVATAPRFVIQNGIVEGYFLSSYSARKLGMQT
                     TGNAGGAHNLYLNHTHETQSDLLKEMGTGLLVTELMGQGANTITGDYSRGAAGFWVEN
                     GVIAYPVHEITVAGRLQDMYRDIVGVADDALRRSSNKIGSILIAGMTVAGS"
     gene            4793..5305
                     /locus_tag="WGQ68_001756"
     CDS             4793..5305
                     /locus_tag="WGQ68_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HEZ0715522.1"
                     /translation="MFEQEDEWVSKTQMKKQMNGLQDLGMELTKLSSDTLKKIGLDED
                     LYEAIVTYKKITSNGALKRQAQFIGRLMRDTDPAPIEAFLAKLRGDDAAHNAFLQRVE
                     RTRMELLADDGALTQFMSDFPNADAGKLRTLIRNTKKEQEQNKPPKNFRALFQELKTV
                     MESQGGTGEA"
     gene            5352..6068
                     /locus_tag="WGQ68_001757"
     CDS             5352..6068
                     /locus_tag="WGQ68_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715523.1"
                     /translation="MLFRKTTAAVLAAALMLNGCTAMMWGMNDPFSQTTAYKHVDKDQ
                     IRAFGVVAKDNAQLEKGSLVMMGGKYWFVVNPEDSAKLTGILKAGLDKPFQIVEDTPS
                     YARHQALPVKLESPGSQNFSTEGLCLRYDTDKPADIAKLKQLGFEAVELDNRTIYTRC
                     VSAKGKYYATPQKLNADYHFEQSVPADIYYTVTKKHTDKSKLFENIAYTPTTLILDAV
                     GAVLALPVAALIAATNSSDK"
     gene            6137..7837
                     /gene="recJ"
                     /locus_tag="WGQ68_001758"
     CDS             6137..7837
                     /gene="recJ"
                     /locus_tag="WGQ68_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEZ0715524.1"
                     /translation="MSVKIQTRSVNTDVFNHLLTAGADPLIARLCASRGVQSPAELDD
                     KLASLLPYQTLTNCEAAARRLADAVERQEKILIVADYDADGATACAVGLDGLAAMGAK
                     VDFLVPNRFEHGYGLTPELAEIAAAQGVDLLITVDNGIASIAGVARAQELGLDVIVTD
                     HHLPAETVPDCIIVNPNQKGCGFQSKSLAGVGVIFYVLMALRAELRRRNYFSDGLKEP
                     NLGDLLDLVALGTVADVVSLDHNNRILVSQGLKRMRSGKMRPGIRALFEVARRDWRKA
                     QPFDMGFALGPRINAAGRLDDMSVGIACLLARDDTEAQELAAQLNNLNIERREIEQSM
                     LQDALNAFPETLPSGQMTLVAYRDDFHQGVVGIVASRLKDRFYRPTIVFAPADNGEVR
                     GSGRSIPNLHLRDALDLVSKRHPDLILKFGGHAMAAGLSILEHNIPAFQTAFEEAVRE
                     MVCEDDLSQTFITDGSLPARDITLEQAQNLAKHVWGQGFAPPSFTDEFHVVRQQPLGA
                     EGKHKKVWLQKDGCEFEAMFWRCSEDIPEYIRTVYRPVANEWRNNLELQLYIDYWEAV
                     "
BASE COUNT         1703 a         2055 c         2339 g         1898 t
ORIGIN      
        1 attttgacct tgcttttctt aaacttcaac acggcttctt cttttgccgt atcccagtct
       61 atccgcacaa agccgccgtc ggcgagtttg ccgaacagga gttcgtcggc gagcggtttg
      121 cggatttttt cctgaatcag gcggtgcatc gggcgcgcgc ccatttgcgg gtcgaaacct
      181 ttttccgcca gatatttgtg caatgccgac gtgaattcgg cttcgacttt tttgtcgagg
      241 agctggtgtt cgagctggag caggaatttg tccacgactt tgacgatgat gggttcggac
      301 aagggcgcaa acgggataat cgcgtccaag cggttgcgga actcgggcgt gaagagcttg
      361 ttgattgcct gcatttcgtc gccgcgttcg cgcttggcgg taaagccgag gctggggcgg
      421 ctgaggcttt ccgcgcctgc gttggtggtc ataatcagga tgacgttgcg gaaatcggca
      481 ctcttgccgt tgttgtcggt cagtttgcct gcgtccatga cttgcaggag gacgttgaaa
      541 atgtcggggt gggctttttc gatttcgtcc aagagcaata cgcaatgcgg ctgtttgttg
      601 acggcttcgg tcaaaaggcc gccttgttca aagccgacgt agcccggtgg cgcgccgatg
      661 aggcgcgata cggcatggcg ttccatgtat tcggacatat caaagcgttg cagcggtacg
      721 cccagcgagt aggcaagctg tttggcggct tcggttttgc ccacgccggt cgggccggag
      781 aagaggaagc tgcctatcgg tttgtcgggc agggcaaggc cggaacgcga cattttgacg
      841 gcggcaacca gcgcgtcgat ggcgttttct tgaccgtaaa ccatgttttt caaatcgcga
      901 ccgaggaatt gcagcacttg tttgtcgtcg tgcgacacgg ttttttcggg aatccgcgaa
      961 actttggcga tgacggtttc gatttgtgct ttgccgatga cttttttctg tttggatttg
     1021 ggcagaatcc gttgcgccgc gcctgcttca tccattacgt cgatggcttt gtcgggcagg
     1081 aaacgctcgt tgatgtagcg tgcggagagt tcggcggcgg cttcgagtgc gccttgagtg
     1141 tagcggactt ggtggaaggc ttcaaacatc ggtttcaagc cgcgcaggat ttgaacggtt
     1201 tcggaaacgg tgggttcgac cacgtcgatt ttttggaagc ggcggcttaa ggcatggtct
     1261 ttgtcgaaaa tggtgcggta ttcgtcgtag gtggtcgcgc cgatgcagcg cagcgaacct
     1321 tttgccagcg cgggtttgag caggttggac gcgtccatgg tgccgccgct ggtgctgccc
     1381 gcgccgatga tggtgtggat ttcgtcgata aacaaaatgg cgtgcgggat tttttcgagc
     1441 tgtttcaaga cggatttgac ccgcgcttca aagtcgccgc ggtatttcgt gcccgccaac
     1501 agcgaaccca tatccagcgc gtacacttcg gtatctttga gcgcgtcggg aatgtcgctg
     1561 ttgacgattt gatgtgccaa accttccgcc agcgcggttt tgcccacacc tgcttcgccg
     1621 accaaaagcg gattgttttt gcggcggcgg cacaggattt gcaccagccg ttccatttcg
     1681 tgtttgcgac caatcaaagg gtcgatacgg ccggctttga cttcggcgtt gaggttgacg
     1741 gtgtagtcgg aaagggagcc ggttttttgt tccgcttctt cgccctcgcg tcccaggccg
     1801 tctgaatcat tgccatcctc gtcatcgggc gagccgtggg caatacagcg caaaacttca
     1861 aaacgcgtaa ccgattgcag cttgaggaaa tagacggtgt ggctgtcggt ttcgctcatc
     1921 agcgcgacca aaacgtccaa cggttcgact gcggcttttc cggcagactg ggtatgcacc
     1981 atcgcccgtt gcatcacgcg ttggaagccg agcgtgggcc gtgtttcgac cgtgtctaaa
     2041 aggtgttcgg gaatcagggg ggtgttttcg gcaacgctgg cggcgagctg ttcggacacc
     2101 actttcaaat ccgcgccgca tagttttaag acgttcggca cggaggcatc ttcttcgatg
     2161 agtaccaaaa gcagatgctc gaggctgata aattcataat gagccttacg cgcctcgcgg
     2221 taaagctgct gcaaaatctg ttccaattcg ggtgcaagca tattaaatct cctcgacaat
     2281 acattgcagc ggatgccctt cggcttttgc ccgctgcatg acttgttgct gtttggtttg
     2341 ggcaatatcg cgcgtgtaag tgccgcacag gcctttgcct tcgtgatgaa ccaagagcat
     2401 taccgctacc gcctgttctt gtccgagcat gaagatttcg gtcaggattt caacgacaaa
     2461 ttccatcgtg gtgtaatcgt cgttcaatag gaaaacgccg taacgtttcg gcggcagggt
     2521 gttcagacgg tgcaagagcg tgtcggattg gtgttgcgcg gtcatagtgt gtcccatttg
     2581 aaagccgcgt tcagacggca tttttgttgt attttcggta cttttgccta ttttcccact
     2641 tttttgaaaa catagcttga cgttttgtct taacaaatgt aaaaagacgg ttaagcagga
     2701 ttgggcatcc gccgagtata tcaagctgga aggaacgcca gacggctcgg tttgccgtat
     2761 gccttgtttt gctgattttg ttaatttttg agtataggaa gtttctaatg gcaaccggta
     2821 tcgtaaaatg gtttaacgac gctaaaggtt ttggtttcat cacgcctgat gaaggcggcg
     2881 aagatttgtt cgctcacttc tcagcaatca atatggaagg tttcaaaacc ctgaaagaag
     2941 gccaacgtgt ctctttcgac gtaaccaccg gccctaaagg caaacaggcc gccaacattc
     3001 aggctgctta attcctgatg tacggtcaaa tgtatatttg aaaacggcgg gacaggcaat
     3061 gtcccgccgt ttttgtctgc cgttttgccg gcggcggaaa aaccccaatc cccgcacgcc
     3121 ttatcctgaa cttgtgtgta ccctgttgtg gacaagtggc ttagtatttt gacggataag
     3181 ggaaaatcag tgctgatgaa aaaatgtgca atgttgtcgg caaaaggcgg tgcggcataa
     3241 aacggcaaac gggcaggcac ggggcaaaac gtgctgcctt cgtcttcaga cggcatcggc
     3301 agggcgttca gcttccggca accgtcatcc ccgcaatcag aatcgagccg attttgttgg
     3361 acgaacgccg caaagcgtca tccgccacgc cgacaatgtc gcggtacata tcctgcaagc
     3421 gtccggctac ggtaatctcg tggacggggt aggcaatcac gccgttttcc acccaaaaac
     3481 ccgccgcgcc gcgcgagtag tcaccggtaa tggtgttcgc gccctgtccc atcagttcgg
     3541 ttaccaacaa tcccgtcccc atttctttca gcaggtcgga ttgcgtttcg tgcgtatggt
     3601 tcaaatacag gttgtgcgcg ccgccggcgt tgcccgtggt ctgcataccg agtttgcgcg
     3661 cgctgtaact gctgaggaaa tagccttcga caatgccgtt ttgaatcacg aagcgcggcg
     3721 cggtggcaac gccttccgca tcaaaatagc tgctgcggaa agagcggggg atgtgcggtt
     3781 cttcgcgcag gttgaggaaa tcgggcagga cttttttgcc gatgctgtcg atcaggaaac
     3841 tgctttggcg gtagagcgcg ccgccggaga gtgcgccgac gaggtgtccg ataagaccgc
     3901 ccgaaacggt ggtatcgaag aggacggggt agctgcctgt cgggatgctg cggctgccga
     3961 gtcggcgcaa agtgcggcgg gcggcggttt gaccgatggt ttcggggctg tccatatccg
     4021 gatggcggca ggcggaatcg taccagtagt cgcgctgcat cccgttttcg tcggcggcaa
     4081 cgacgctgca ggaaatgctg tggtgcgtgc cttgccggtg tgcggcaaaa ccgtgggtgt
     4141 tgccgtaaac gtattggtaa tggccggttt gcaccgccgc gccttcggag ttttcgatgc
     4201 gctcatcctc gttcagggcg gcttgttcgc attgttttgc aaagccgacg gcggcttccg
     4261 tatccaaatc ccattcgtgg taaaggtcgg ggtcgccgat gtgttgcgcc atcaactcgg
     4321 ggtcggcaag tccggcgcaa ccgtcttcgg cggtgtggcg ggcgatgtcg gcggcggcgc
     4381 ggacggtgtc gcgcagggct tgttcggaga aatcggcggt gccagcgcgg cctttgcgtt
     4441 tgccgacgta aacggtaatg tccagcgact tgtcctgctg gaactcgatt tgttcgattt
     4501 cgcccagccg cacgctgacg ctttgtccga tggattcgct gaaatcggct tcggcggcgg
     4561 tcgcgcccgc tgcttttgcc aagtcgagcg tgcggcggca gaggtcgaga agttcggaag
     4621 cggtgtggtt gaacagcata gaaatctttc ttggtggagc gttgcggcat tttaaccgtt
     4681 tcgggcggca ggggcaaaag cgtccgtttg cagggcggac ggtgcaaaat gccgtctgaa
     4741 cgcggaatca ttctgttaaa atgcgcccgt cggataaaat ttgagaatga agatgtttga
     4801 acaagaagac gaatgggtca gcaaaaccca aatgaaaaag cagatgaacg gtttgcagga
     4861 tttgggtatg gagctgacca agctctcaag cgacacgttg aaaaaaatcg gcttggacga
     4921 agatttgtac gaagccatcg ttacctataa aaaaatcaca tccaacggcg cgctcaagcg
     4981 gcaggcgcaa tttatcggca gactgatgcg cgataccgat cccgcgccca tcgaggcatt
     5041 tcttgccaag ctgcgcggcg acgatgcggc gcacaacgcc tttttgcaac gtgtggagcg
     5101 gacgcggatg gagctgctgg cggacgacgg cgcgctgacg cagtttatgt ccgattttcc
     5161 aaacgccgat gcgggcaagc tgaggacgct catccgcaat accaaaaaag aacaggaaca
     5221 gaacaaaccg ccgaaaaatt tccgcgccct gtttcaagag ttgaaaaccg ttatggaaag
     5281 ccaaggcgga acaggggaag cctaggcata ttttcagacg gcatccgccg tcattctgat
     5341 tggaagataa aatgttgttc cgtaaaacga ccgccgccgt tttggcggca gccttgatgt
     5401 tgaacggctg tacggcaatg atgtggggca tgaacgaccc gttcagccaa acgaccgcct
     5461 ataaacacgt tgacaaggat caaatccgcg ccttcggtgt ggttgccaaa gacaatgccc
     5521 aattggaaaa gggcagtctg gtgatgatgg gcgggaaata ctggttcgtc gtcaatcccg
     5581 aagattcggc gaagctgacg ggcattttga aggcagggct ggacaaaccc ttccaaatag
     5641 ttgaggatac cccgagctat gctcgccacc aagccctgcc ggtcaaactc gaatcgcctg
     5701 gcagccagaa tttcagtacc gaaggccttt gcctgcgcta cgataccgac aagcctgccg
     5761 acatcgccaa gctgaaacag ctcgggtttg aagcggtcga acttgacaat cggactattt
     5821 acacgcgctg cgtatccgcc aaaggcaaat actacgccac accgcaaaaa ctgaacgccg
     5881 attatcattt tgagcaaagt gtgcctgccg atatttatta cacggttacg aaaaaacata
     5941 ccgacaaatc caagttgttt gaaaatattg catatacgcc caccacgttg atactggatg
     6001 cggtgggcgc ggtgctggcc ttgcctgtcg cggcgttgat tgcagccacg aattcctcag
     6061 acaaatgaac ggcaatgccg tctgaaaagc cttcagacgg catttcaaac acatccgcac
     6121 tgtaaagttc cacgctatgt ccgtcaaaat ccaaacccga tccgtcaata ccgacgtttt
     6181 caatcatttg ctcaccgccg gcgccgatcc tttaatcgcc cggctttgtg cctcgcgcgg
     6241 tgtgcaaagt cctgccgaat tggacgacaa actcgcttcc ctcctgcctt atcaaacgct
     6301 gacgaattgc gaagccgccg cccgccgttt ggcggatgcg gttgagcgtc aggaaaaaat
     6361 cttgattgtc gccgactacg atgccgacgg cgcgaccgcg tgtgccgtcg gtttggacgg
     6421 tttggcggcg atgggggcaa aagtagattt cctcgtgccc aaccgctttg aacacggcta
     6481 cggtttaacg cccgagctgg ccgaaatcgc cgccgcgcaa ggcgtggatt tgctgattac
     6541 ggttgataac ggcattgcca gcatcgcagg cgtggcgcgt gcgcaggaat tgggtttgga
     6601 tgtgattgta accgaccacc atctgcctgc cgagaccgtg ccagactgca tcatcgtcaa
     6661 tccgaaccaa aaaggctgcg gtttccaaag caaaagcttg gcgggcgtgg gcgtgatttt
     6721 ttatgtactg atggctttgc gtgccgaact gcgccgccgc aattattttt cagacggcct
     6781 aaaagagccg aatctgggcg accttttgga tttggtcgca ctcggtaccg ttgccgacgt
     6841 cgtctccctc gatcacaaca accgcatcct cgtgtcgcaa ggtttgaaac ggatgcgctc
     6901 cggcaaaatg cgccccggta tccgcgcctt gtttgaagtg gcgcggcgcg attggcgcaa
     6961 ggcgcagccg tttgatatgg gttttgcgtt gggcccgcgc atcaacgccg ccggacggct
     7021 ggacgatatg tcggtcggta ttgcctgcct gttggcgcga gatgataccg aggcgcagga
     7081 actggcggcg cagttaaaca acctcaatat cgaacgtcgc gaaatcgagc agtctatgct
     7141 gcaagacgcg ctgaatgctt ttcctgaaac cctgccttca ggtcagatga ctttggtggc
     7201 gtatcgcgac gacttccatc aaggcgtggt cggcatcgtt gccagccgcc tcaaagaccg
     7261 tttttatcgt ccgaccatcg tgtttgcgcc tgccgacaac ggtgaagtgc gcggttcggg
     7321 gcgttccatt cccaatcttc acttgcgcga tgctttggac ttggtgtcca aacgccatcc
     7381 cgatttgatt ttgaaattcg gcggacacgc gatggcggcg ggcttgagca tactcgaaca
     7441 caacattccc gcgtttcaga cggcctttga agaagccgtg cgcgaaatgg tgtgcgaaga
     7501 cgatttgtcg caaaccttca tcaccgacgg cagcctgcct gcacgcgaca ttacgctgga
     7561 acaggcgcaa aaccttgcta aacacgtttg ggggcagggc ttcgcgccgc cgagttttac
     7621 cgacgagttc cacgtcgtcc gccagcaacc tttgggcgcg gagggcaaac acaaaaaagt
     7681 ctggctgcaa aaagacggct gcgaatttga agcgatgttt tggcgttgca gcgaagacat
     7741 ccccgaatac atccgcacgg tgtaccgccc cgttgccaac gaatggcgga ataatctcga
     7801 attgcagctt tatatcgact actgggaagc ggtgtagagg cggcggaacg ccgtttgaat
     7861 gtgatttctg ttccttcatt tgcctgttag tacgacggga atgttcccaa tcggaaaaga
     7921 aagtaaaggg cgttaatttt tgttaatcat cctttcttag ggacgcaata tataaggtgc
     7981 atcaaatttc agact
//
LOCUS       DAWFHY010000089         7965 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.089, whole genome shotgun sequence.
ACCESSION   DAWFHY010000089 DAWFHY010000000
VERSION     DAWFHY010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7965
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.089"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(173..589)
                     /gene="dksA"
                     /locus_tag="WGQ68_001759"
     CDS             complement(173..589)
                     /gene="dksA"
                     /locus_tag="WGQ68_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEZ0715525.1"
                     /translation="MAKLTEQDILNWNGPEDDYMNDEHLAFFRELLVKMQDELIENAS
                     ATTGHLQEHESAPDPADRATQEEEYALELRTRDRERKLLSKIQATIRNIDEGDYGFCA
                     DTGEPIGLKRLLARPTATLSVESQERRERMKKQFAD"
     gene            complement(736..1527)
                     /locus_tag="WGQ68_001760"
     CDS             complement(736..1527)
                     /locus_tag="WGQ68_001760"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEZ0715526.1"
                     /translation="MNVYFLGGGNMAAAIAGGLVKQGGYRIYIANRGAEKRERLEKEL
                     GVETSATLPELHSDDVLILAVKPQDMEAACKNIRTNGALVLSVAAGLSVDTLSRYLGG
                     NRRIVRVMPNTPGKIGLGVSGMFAEAEVSETDRRIADRIMKSVGLTVWLEDEAQMHSI
                     TGISGSGPAYVFYLLDALQNAAIRQGFDMAEARALSLATFKGAVALAEQTGEDFEKLQ
                     KNVTSKGGTTHEAVEAFRRHRVAEAISEGVCACVRRSQEMERQYQ"
     gene            complement(1576..1983)
                     /locus_tag="WGQ68_001761"
     CDS             complement(1576..1983)
                     /locus_tag="WGQ68_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEZ0715527.1"
                     /translation="MEIRAIKYTAMAALLAFTVAGCRLAGWYECSSLSGWCKPRKPAA
                     IDFWDIGGESPPSLEDYEIPLSDGNRSVRANEYESAQQSYFYRKIGKFEACGLDWRTR
                     DGKPLIETFKQEGFDCLKKQGLRRNGLSERVRW"
     gene            complement(1983..2669)
                     /locus_tag="WGQ68_001762"
     CDS             complement(1983..2669)
                     /locus_tag="WGQ68_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEZ0715528.1"
                     /translation="MTVLQERYCEVSDRIGKLVLQAGREPHSVSLIAVGKTFPSDGIR
                     EVYAAGQRDFGENYIQEWYGKTEELADLTDIVWHVIGDVQSNKTKFVAERAHWVHTVC
                     RLKTAVRLSEQRPSSMPPLQVCIEVNIAGEAAKHGVAPEEAVALAVEVAKLPNIVVRG
                     LMCVAKANSSETELKVQFQTMRKLLSDLNTAGVKADVLSMGMSDDMPAAIECGATHVR
                     IGSAIFGKRG"
     gene            2789..3832
                     /locus_tag="WGQ68_001763"
     CDS             2789..3832
                     /locus_tag="WGQ68_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011798741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEZ0715529.1"
                     /translation="MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSA
                     EEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVARFRVNAFNIGRGPAAVFRTIPST
                     VLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
                     PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAA
                     ETGHLVFGTLHTTGAAKTVDRIVDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNG
                     RVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQSLQSLVRQGLIAPEV
                     ARRRAQNSESMSF"
     gene            3995..5221
                     /locus_tag="WGQ68_001764"
     CDS             3995..5221
                     /locus_tag="WGQ68_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEZ0715530.1"
                     /translation="MNTDNLHDILDEMVQVYSQKKQSRSETPAEIGAHFHPLLDRLCE
                     TAEAQNASDILISKGFPPSLKINSALTPQPQKALTGEETAAIAASTMNAEQSEIFRRD
                     GEINYSVQSRSGTRYRANAYHSQGSAGLVLRRINHVIPQMQELGLPEKLKDLAVASRG
                     LLIIVGPTGSGKSTTMATMLEHRNKTLPSHIVTIEDPIEFIYKPRRCIFTQREIGVDT
                     INWQTAVQNAMRQSPDVVCIGEVRSRESMEYAMQLAQTGHLCIFTLHANTAPQSLERI
                     LNFYPKEQHNQILIDIALNLTGIICQRLALKQDKTGRTAVVDLLINTPAIQDFILKGD
                     LMNISKIMETAKTDGMQTMDQNLFELYRHGIISYEEALRQSVSANNLRLHIQLHKEGK
                     TPELLYDRVNGLNLIS"
     gene            5507..7657
                     /gene="yccS"
                     /locus_tag="WGQ68_001765"
     CDS             5507..7657
                     /gene="yccS"
                     /locus_tag="WGQ68_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HEZ0715531.1"
                     /translation="MKTPLLKPLLITSLPVFASVFTAASIVWQLGEPKLAMPFVLGII
                     AGGLVDLDNRLTGRLKNIIATVALFTLSSLTAQSTLGTGLPFILAMTLMTFGFTILGA
                     VGLKYRTFAFGALAVATYTTLTYTPETYWLTNPFMILCGTVLYSTAIILFQIILPHRP
                     VQESVANAYDALGGYLEAKADFFDPDEAAWIGNRHIDLAMSNTGVITAFNQCRSALFY
                     RLRGKHRHPRTAKMLRYYFAAQDIHERISSAHVDYQEMSEKFKNTDIIFRIHRLLEMQ
                     GQACRNTAQALRASKDYVYSKRLGRAIEGCRQSLRLLSDGNDSPDIRHLRRLLDNLGS
                     VDQQFRQLQHNGLQAENDRMGDTRIAALETGSLKNTWQAIRPQLNLESGVFRHAVRLS
                     LVVAAACTIVEALNLNLGYWILLTALFVCQPNYTATKSRVRQRIAGTVLGVIVGSLVP
                     YFTPSVETKLWIVIASTTLFFMTRTYKYSFSTFFITIQALTSLSLAGLDVYAAMPVRI
                     IDTIIGASLAWAAVSYLWPDWKYLTLERTAALAVCSNGAYLEKITERLKSGETGDDVE
                     YRATRRRAHEHTAALSSTLSDMSSEPAKFADSLQPGFTLLKTGYALTGYISALGAYRS
                     EMHEECSPDFTAQFHLAAEHTAHIFQHLPETEPDDFQTALDTLRGELDTLRTHSSGTQ
                     SHILLQQLQLIARQLEPYYRAYRQIPHRQPQNAA"
BASE COUNT         1965 a         2612 c         1717 g         1671 t
ORIGIN      
        1 gtatacggtc aatacttcat cttgcttttt ttaattattt tctccatctc gttgtctgat
       61 aaggtttttt atttcgccac aaaccacatc cgactcgaaa tttacctgcc gccatcttaa
      121 tctgcttccc tttttttctg tcatatggtt tgaaaataac caaaaggcaa atttagtcgg
      181 caaactgttt tttcatcctc tctcggcgtt cttgggactc aacagataaa gtggctgtcg
      241 ggcgtgccag cagccgcttc agaccgatag gctctcccgt atcggcacag aatccataat
      301 ccccttcatc aatattgcgg atggtcgcct gtattttact gagaagtttt cgttcccgat
      361 cgcgggtacg gagttccaat gcgtactctt cttcctgtgt ggcacggtcg gcaggatcgg
      421 gggctgattc gtgttcttgg agatgccctg tcgtagcgga agcattttcg atgagttcgt
      481 cttgcatttt taccagcaat tcgcggaaaa aagccaaatg ctcgtcattc atataatcgt
      541 cttccggccc gttccaattc aaaatatctt gttctgtcag ctttgccata atgtatatcc
      601 tctcggttca ccgaaaaaaa caaagcacca caaagtggct ttattccttt gatttttata
      661 aggcttattg cctaaggcgt ttttgaaaac gcgcatcata acacgttttg gattggtttt
      721 tttattttct ttacattatt gatattgccg ttccatttcc tgcgaacggc gcacacaggc
      781 acaaacgccc tcacttatgg cttcggcgac acgatgccgc ctgaaggctt ccacggcttc
      841 gtgggttgtc ccgccttttg acgttacatt tttttgaagc ttctcgaaat cttcacccgt
      901 ctgctcggca agggcaaccg ctcctttaaa cgttgccaga ctgagcgcgc gtgcttctgc
      961 catatcaaac ccttgtcgga tggcggcatt ttgcaatgcg tccagcagat aaaacacata
     1021 tgccggcccg ctgccgctga taccggtaat gctgtgcatt tgcgcttcat cttccaacca
     1081 aacggtcaaa ccgactgatt tcatgattcg atcggcaatc ctgcggtctg tttccgatac
     1141 ttccgcttcg gcaaacatac cggatacgcc cagcccgatt tttccgggtg tattcggcat
     1201 aacccggaca atgcggcggt ttcccccgag gtaacggctg agcgtatcaa ccgacaatcc
     1261 agctgcaaca gaaagcacca atgcgccgtt ggtgcggata tttttgcacg cggcttccat
     1321 atcctgcggt ttgacggcaa ggattaaaac atcgtcggaa tgaagctccg gcagggttgc
     1381 cgaagtttcg acccccaact ctttttccaa acgttcgcgt ttttccgcac cccgattggc
     1441 tatatagatg cggtaaccgc cttgtttgac caatccgcct gcgatggcag ccgccatatt
     1501 gccgccgccg agaaaataaa cattcattat ttctacccta ttcaaaatta ccttactaaa
     1561 ttcattccca attttttacc atcggacgcg ctcggacaga ccgttgcgcc gcaacccctg
     1621 ctttttcaaa caatcaaaac cttcctgttt gaacgtctca atcaaaggtt tgccatcacg
     1681 cgtacgccaa tccaacccgc aggcttcaaa cttccctatt ttcctgtaaa agtaagattg
     1741 ttgtgcggat tcatattcgt ttgccctgac ggaacgattg ccgtctgaaa gcggtatctc
     1801 gtagtcctct aaagacggcg gactctcgcc gccaatatcc caaaaatcga tggcggcagg
     1861 ttttctcggc ttacaccagc cggacaggga cgaacactca taccaacctg ccaaccggca
     1921 gcctgcaacc gtaaatgcaa gcaacgcagc cattgccgta tattttattg cccgaatttc
     1981 catcagcccc ttttcccgaa aatcgcgctg ccgatacgga cgtgtgtcgc accgcactca
     2041 atggcggcag gcatatcgtc cgacatcccc atagacagca cgtccgcctt aacgccagcc
     2101 gtattgaggt cggaaagcag tttccgcatc gtttgaaatt gcaccttcaa ctccgtttca
     2161 ctgctgttgg ctttggcaac acacatcaag ccgcgtacga cgatattcgg cagcttcgcc
     2221 acttccacag caagcgcgac tgcttcttcg ggcgcaacac cgtgctttgc cgcctcgccc
     2281 gcaatattca cctcgataca cacctgcaaa ggcggcattg aggaaggacg ctgttcgctc
     2341 aaccggacag cggttttcag acggcatacg gtatgcaccc aatgcgcgcg ttcggcgaca
     2401 aacttggttt tgttggactg cacatcgccg atgacgtgcc acacgatgtc ggtcaaatcc
     2461 gccaactctt ccgttttgcc gtaccactcc tgaatatagt tctcgccgaa atcacgctgt
     2521 ccggcggcgt aaacttcgcg gatgccgtct gaagggaaag tcttaccgac ggcaatcagg
     2581 ctgacggaat gcggctccct gcccgcctgc agaaccaatt ttccgatacg gtcggacacc
     2641 tcacaataac gttcttgcaa caccgtcata gattatcccc taattaaaaa tgattaaaca
     2701 gttgaaaccc ctccagtcag ggcggtacaa tcaaggttgt tagaaccatt ccaaccaatc
     2761 gaaacattat actaaacaga gccgcattat gcagattacc gacttactcg ccttcggcgc
     2821 taaaaacaaa gcatccgacc ttcacctgag ttcgggcata tcccctatga ttcgggttca
     2881 cggcgatatg cggcgcatca accttcccga aatgagcgcg gaagaggtcg gtaatatggt
     2941 aacttcggtg atgaacgacc accagcggaa aatctaccag caaaacttgg aagttgactt
     3001 ctcgttcgaa ctgcccaacg tcgcccgatt ccgcgtcaac gccttcaaca tcggccgcgg
     3061 tcccgccgcc gtattccgca ccattcccag caccgtctta tcgctggaag aattgaaagc
     3121 cccgagcatt ttccaaaaaa tcgcagaatc gccgcgcggc atggttttgg ttaccggccc
     3181 taccggttcg ggcaaatcga ccacgcttgc cgcaatgatc aactacatca acgaaaccca
     3241 gccggcacac atcctgacca tcgaagaccc gatcgaattc gtccaccaaa gcaaaaaatc
     3301 cctgattaac cagcgcgagc tgcaccagca caccctcagc ttcgccaacg cgctgcgttc
     3361 cgcattgcgc gaagaccccg acgttatcct tgttggcgag atgcgcgacc ccgaaaccat
     3421 cggcttggca ctgaccgccg ccgaaaccgg acacttggtt ttcggcacgc tgcacacgac
     3481 cggcgcagca aaaaccgtcg accgtatcgt ggacgtattc ccagcgggag aaaaagaaat
     3541 ggtgcgctct atgctgtccg aatcgctgac cgccgtcatc tcccaaaacc tgctgaaaac
     3601 gcacgacggc aacggccgtg tcgcctcgca cgaaatcctg attgccaacc ccgccgtccg
     3661 caacctcatc cgcgaaaaca aaatcacgca gattaactcc gtcctgcaaa ccgggcaggc
     3721 gagcggtatg cagacgatgg accaatcgct gcaatcgctg gtgcgccaag ggctgatcgc
     3781 accggaagtc gcacgcagac gcgcgcaaaa cagcgaaagt atgagtttct gacacacaac
     3841 cgccttccgg ccataccggc gggaaaacaa ggcgcaaaca cgcggggcgg gacgcagcat
     3901 cccgcccggc tacctttccg aacaggaagc gtccgccttc ctgttgaaac ctgccgccgc
     3961 aaactgcaag gcttaaaccg aaaagaagct aacgatgaat accgataacc tgcacgacat
     4021 cttggacgaa atggttcaag tgtattctca aaaaaaacaa agccgatccg aaaccccggc
     4081 cgaaatcggc gcacacttcc acccgctgct cgaccgcctg tgcgaaaccg cagaagcaca
     4141 aaacgcgtcc gacatcctta tcagcaaagg attcccgccc tcgttgaaaa tcaacagcgc
     4201 attaaccccg cagccgcaaa aggcgctgac gggcgaggaa accgccgcca tcgccgcatc
     4261 gacgatgaac gccgaacaat cggaaatatt ccggcgcgac ggcgaaatca actactccgt
     4321 ccagtcgcgc agcggcacgc gctaccgcgc caacgcctac cacagccaag gcagcgcagg
     4381 tttggttttg cggcgcatca accacgtcat cccgcaaatg caggaattgg gcctgcccga
     4441 aaaactcaaa gacctcgccg tcgcatcgcg cgggctgctg attatcgtcg ggcctaccgg
     4501 ttcgggcaaa tccaccacga tggcgactat gctcgaacac cgcaacaaaa ccctgcccag
     4561 ccatatcgtt accatcgaag acccgattga gtttatctac aaaccgcgcc gctgcatctt
     4621 tacccagcgc gaaatcggcg tcgacaccat aaactggcag acggcggtac aaaacgctat
     4681 gcgccaatcc cccgacgtgg tctgcatcgg cgaagtccgc agcagggaaa gtatggaata
     4741 cgcgatgcag ctcgcccaaa ccggccacct gtgcattttt acgctccacg ccaacaccgc
     4801 gccgcagtcg ctcgaacgca tactcaactt ctaccccaaa gaacagcaca accaaatact
     4861 gatcgacatc gccctcaacc tgaccggcat catctgccaa cgcctcgccc tcaaacaaga
     4921 caaaacgggc aggacggcgg ttgtcgactt gctcatcaac acgcccgcca tccaagactt
     4981 catcctgaag ggcgacctga tgaacatcag taaaatcatg gaaaccgcca aaaccgacgg
     5041 aatgcagacg atggatcaaa accttttcga actgtaccgt cacggcatca tcagttacga
     5101 agaagccctg cgccagtccg tttccgccaa caacctgcga ttgcacatcc aactgcacaa
     5161 agaaggcaaa acgcccgaac tcctttacga cagggtcaac ggtctcaacc tcatttcctg
     5221 atccgcaaaa cccaatgccg tctgaaaacc gcatccccgt tttcagacgg catgatttta
     5281 tccgccccat tcatgtgcta cactttatag tggattaaat ttatagtgga ttaacaaaaa
     5341 tcaggacaag gcgacgaagc cgcagacagc acaaatagta cggaatcgat tcacttggtg
     5401 cttgagcacc ttagagaatc gttctctttc agctaaggcg aggcaacgcc gtactggttt
     5461 ttgttaatcc actatattca cttcataaca ataaaccggt aaaaccatga aaaccccact
     5521 cctcaagcct ctgctcatta cctcgcttcc cgttttcgcc agtgttttta ccgccgcctc
     5581 catcgtctgg cagctaggcg aacccaagct cgccatgccc ttcgtactcg gcatcatcgc
     5641 cggcggcctt gtcgatttgg acaaccgcct gaccggacgg ctgaaaaaca tcatcgccac
     5701 cgtcgccctg ttcaccctct cctcgctcac ggcacaaagt accctcggca cagggctgcc
     5761 cttcatcctc gccatgaccc tgatgacctt cggctttacc attttaggcg cggtcgggct
     5821 gaaataccgc accttcgcct tcggcgcact cgccgtcgcc acctacacca cacttaccta
     5881 cacccccgaa acctactggc tgaccaaccc cttcatgatt ttgtgcggca ccgtactgta
     5941 cagcaccgcc atcatcctgt tccaaatcat cctgccccac cgccccgtcc aagaaagcgt
     6001 cgccaacgcc tacgacgcac tcggtggcta cctcgaagcc aaagccgact tcttcgaccc
     6061 cgatgaggca gcctggatag gcaaccgcca catcgacctc gccatgagca acaccggcgt
     6121 catcaccgcc ttcaaccaat gccgttccgc cctgttttac cgccttcgcg gcaaacaccg
     6181 ccacccgcgc accgccaaaa tgctgcgcta ctacttcgcc gcccaagaca tacacgaacg
     6241 tatcagctcc gcccacgtcg actaccaaga gatgtccgaa aaattcaaaa acaccgacat
     6301 catcttccgc atccaccgcc tgctcgaaat gcagggacaa gcctgccgca ataccgccca
     6361 agccctgcgc gcaagcaaag actacgttta cagcaaacgc ctcggccgcg ccatcgaagg
     6421 ctgccgccaa tcgctgcgtc tcctttccga cggcaacgac agccccgaca tccgccacct
     6481 gcgccgtctt ctcgacaacc tcggcagcgt cgaccaacaa ttccgccaac tccagcacaa
     6541 cggcctgcag gcagaaaacg accgcatggg cgacacccgc atcgccgccc tcgaaaccgg
     6601 cagcctcaaa aacacctggc aggcaatccg tccgcagcta aacctcgaat caggcgtatt
     6661 ccgccatgcc gtccgcctgt cccttgtcgt tgccgccgcc tgcaccatcg tcgaagccct
     6721 caacctcaac ctcggctact ggatactact gaccgccctt ttcgtctgcc aacccaacta
     6781 caccgccacc aaaagccgcg tccgccagcg catcgccggc accgtactcg gcgtaatcgt
     6841 cggctcgctc gtcccctact ttaccccctc cgtcgaaacc aaactctgga tcgtcatcgc
     6901 cagtaccacc ctctttttca tgacccgcac ctacaaatac agtttctcga catttttcat
     6961 caccattcag gcactgacca gcctctccct cgcaggtttg gacgtatacg ccgccatgcc
     7021 cgtgcgcatc atcgacacca ttatcggcgc atcccttgcc tgggcggcag tcagctacct
     7081 gtggccagac tggaaatacc tcacgctcga acgcaccgcc gcccttgccg tatgcagcaa
     7141 cggtgcctat ctcgaaaaaa tcaccgaacg cctcaaaagc ggcgaaaccg gcgacgacgt
     7201 cgaataccgc gccacccgcc gccgcgccca cgaacacacc gccgccctca gcagcaccct
     7261 ttccgacatg agcagcgaac ccgcaaaatt cgccgacagc ctgcaacccg gctttaccct
     7321 gctcaaaacc ggctacgccc tgaccggcta catctccgcc ctcggcgcat accgcagcga
     7381 aatgcacgaa gaatgcagcc ccgactttac cgcacagttc cacctcgccg ccgaacacac
     7441 cgcccacatc ttccaacacc tgcccgaaac cgaacccgac gactttcaga cagcactgga
     7501 tacactgcgc ggcgaactcg acaccctccg cacccacagc agcggaacac aaagccacat
     7561 cctcctccaa cagctccaac tcatcgcccg gcagctcgaa ccctactacc gcgcctaccg
     7621 acaaattccg cacaggcagc cccaaaacgc agcctgaaaa agtttcggca ttttgtaaga
     7681 gagagcagat tgtcagacag gttgcaagat agtggataaa gctttgcgca gggtaaatgc
     7741 gtagcaactg aaccgtcatt cccacgaatc tacattccgt cattcccacg aaaacagaaa
     7801 accaaaaaca gaaacctaaa atcccgtcat tcccacgaaa gtgggaatcc agttcgttcg
     7861 gtttcgcttg ttttaagttt cgggtaactt ccacttcgtc attcccgcgc aggcgggaat
     7921 ccaatgcgtt gagcttcagc tatttagaat aaattttgaa actct
//
LOCUS       DAWFHY010000090         7806 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.090, whole genome shotgun sequence.
ACCESSION   DAWFHY010000090 DAWFHY010000000
VERSION     DAWFHY010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7806
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.090"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            307..1461
                     /gene="obgE"
                     /locus_tag="WGQ68_001766"
     CDS             307..1461
                     /gene="obgE"
                     /locus_tag="WGQ68_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEZ0715532.1"
                     /translation="MKFIDEAKIEVAAGKGGNGATSFRREKFVPRGGPDGGDGGKGGS
                     VWAEADENTNTLVEYRFVKRYQAKNGEKGHGSDRYGAGADDIVLKMPVGTLIRDLDTD
                     EIVADLTYHGQRVCLAKGGKGGLGNIHFKSSVNRAPKQSTPGEEGETRSLQLELKVLA
                     DVGLLGMPNAGKSTLITAVSAARPKIANYPFTTLHPNLGVVRIDENHSFVMADIPGLI
                     EGAAEGAGLGHRFLKHLSRTGLLLHVVDLAPFDEAVDPAEEALAIINELRKYDEELYG
                     KPRWLVLNKLDMLDEEEAQERTVTFLEAVGWDYPKPDDRFQFDMETPRLFQISALTHQ
                     GTQELVHQINQYLTEKKRLEAEKAEAEKAAANVEIIEQQPKTDTGVFKPE"
     gene            1544..2593
                     /locus_tag="WGQ68_001767"
     CDS             1544..2593
                     /locus_tag="WGQ68_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEZ0715533.1"
                     /translation="MSSNQYIQSLELTNFTVLPNDTFEFSENLNVIVAENGCGKTHLL
                     KILYSLLEVTSNTKNRLLKTELQKSFADKLLNVFRPDSLGRLSKRLQGRGRTEIVLKL
                     QNGTTHSSLNFSSNSSSQVNVQSIGLKENEYTPTPIFLPSRELITLCPWFTSLYQNQS
                     IPFEETWFDACMQLNHPLAKGPRETKIRELLEPIENAMGGKVSEEQGRFYLSLTNTGG
                     KIEAPLVAEGLRKFVMIARLIATGALLDKGYLFWDEPEANLNPKLIKVAARIIWSLSQ
                     QGIQVFIATHSLFLLRELELLKLENNTDLPVRFFSLIASDDGTKVEQGNSINDLNTLI
                     LLDENIMQADRYLNI"
     gene            3281..4702
                     /locus_tag="WGQ68_001768"
     CDS             3281..4702
                     /locus_tag="WGQ68_001768"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEZ0715534.1"
                     /translation="MTTIYNTLTRQKEPFAPIDPENVRMYVCGMTVYDYCHLGHARVM
                     VVFDMIARWLRECGYPLTYVRNITDIDDKIIARAAENGETIGELTARFIQAMHEDADA
                     LGVLRPDIEPKATENIPQMIAMIETLIQNGKAYPAANGDVYYAVREFSAYGQLSGKSL
                     DDLRAGERVEVDGFKRDPLDFVLWKAAKAGEPAWESPWGNGRPGWHIECSAMSENLFG
                     DTFDIHGGGADLQFPHHENEIAQSVGASGHTCGHDHAQTHHGQSIASHVKYWLHNGFI
                     RVDGEKMSKSLGNFFTIREVLKQYDPEVVRFFILRAHYRSPLNYSDAHLDDAKGALTR
                     LYTTLKNTPAAAFDLSENANDYTRRFYAAMNDDFGTVEAVAVLFELAGEVNKTNDAQL
                     AGCLKALGGIIGLLQRDPTEFLQGGAASDGLSNEEIEDLIARRKQARADKNWAESDRI
                     RDLLNEHKIILEDNAGGTTWRRG"
     gene            4785..5564
                     /gene="xth"
                     /locus_tag="WGQ68_001769"
     CDS             4785..5564
                     /gene="xth"
                     /locus_tag="WGQ68_001769"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEZ0715535.1"
                     /translation="MLKIISANVNGIRSAYKKGFYEYIAASGADIVCVQELKAQEADL
                     SADMKNPHGMHGHWHCAEKRGYSGVAVYSKRKPDNVQIGMGIEEFDREGRFVRCDFDR
                     LSVISLYLPSGSSAEERQQVKYRFLDAFYPMLEAMKNEGRDIVVCGDWNIAHQNIDLK
                     NWKGNQKNSGFLPEEREWIGKVIHKLGWTDMWRTLYPDVPGYTWWSNRGQAYAKDVGW
                     RIDYQMVTPELAAKAVSAHVYKDEKFSDHAPLVMEYDYAAE"
     gene            5551..5895
                     /locus_tag="WGQ68_001770"
     CDS             5551..5895
                     /locus_tag="WGQ68_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715536.1"
                     /translation="MLPNKVLGKYDWNVDGKTGIGAAWVVAAFILPILVWAIFMLSRM
                     QGWLAPTKANPTWALVWLLICMPCLLIASKCLGWKGWRRVVNIFACLTVCAILSVPAS
                     LLIAFTLRDLLK"
     gene            5906..6436
                     /locus_tag="WGQ68_001771"
     CDS             5906..6436
                     /locus_tag="WGQ68_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEZ0715537.1"
                     /translation="MQGFELEDLTLWLIRDAGEDQMWIDRWAISYPVVQMSEASAGQS
                     IGEWQAGLRTAFERIRGKYVAVVAHGAGAAAFLAWLYQADILTRKKIANIILVPQRPD
                     IFPDDAEHTFQRVRCPCRAALVVSEHGDVPRDWAQKQADTWNARLLVSPHSGSLNGML
                     GGWQWGMKLMQEMLLA"
     gene            complement(6665..7780)
                     /gene="asd"
                     /locus_tag="WGQ68_001772"
     CDS             complement(6665..7780)
                     /gene="asd"
                     /locus_tag="WGQ68_001772"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEZ0715538.1"
                     /translation="MKVGFVGWRGMVGSVLMQRMKEENDFAHIPEAFFFTTSNVGGAA
                     PDFGQAAKTLLDANNVAELAKMDIIVTCQGGDYTKSVFQALRDSGWNGYWIDAASSLR
                     MKDDAIIVLDPVNRNVIDNGLKNGVKNYIGGNCTVSLMLMALGGLFQNDLVEWATSMT
                     YQAASGAGAKNMRELISGMGAVHAQVADELADPAGSILDIDRKVSDFLRSEDYPKANF
                     GVPLAGSLIPWIDVDLGNGQSKEEWKGGVETNKILGRSDNPTVIDGLCVRVGAMRCHS
                     QAITLKLKKDLPVSEIETILAGANDWVKVIPNEKEASIHELTPAKVTGTLSVPVGRIR
                     KLGMGGEYISAFTVGDQLLWGAAEPLRRVLRIVLGSL"
BASE COUNT         2050 a         1944 c         2006 g         1806 t
ORIGIN      
        1 cgagtttatc ctagttatct aggacagccc caaaacttat tatatgtaac ggtttgaacg
       61 gacattccgt cgggtcggaa tgtcaaaaag gcgcgattgt accaaagaag ttggacataa
      121 ttttgtttgc aggctgaaga tttgcttaaa aattcattaa gatgggcgga acaaatagtt
      181 tgggtacggc gtgttgaaat accgcttatc ccttataata gcgtccgaaa ttctgttcgg
      241 acggcatcaa aacacatggt aatccgtctg aaccccccat ttgaaaccaa caaacaagag
      301 cattgaatga aattcatcga cgaagcaaaa atcgaagtcg ccgcaggcaa aggcggtaat
      361 ggcgcaacca gtttccgccg cgaaaaattc gtaccgcgcg gcggcccgga cggcggcgac
      421 ggcggcaaag gcggtagcgt ctgggcggaa gccgacgaaa acaccaacac cctcgtcgaa
      481 taccgcttcg ttaaacgcta ccaagccaaa aacggcgaaa aaggccacgg ttccgaccgt
      541 tacggcgcag gtgcggacga catcgtcctc aaaatgcccg tcggcaccct tatccgcgac
      601 ctcgacaccg acgaaatcgt tgccgacctc acttaccacg gacagcgcgt ctgcctagcc
      661 aaaggcggca aaggcggctt gggcaacatc cacttcaaat cctccgtcaa ccgcgcccca
      721 aaacaatcca cgcccggcga agaaggcgaa acccgctccc tgcaactcga actcaaagtc
      781 ctcgccgatg tcggcttatt aggcatgccc aacgccggca aatccaccct gattaccgcc
      841 gtatccgccg cacgccccaa aatcgccaac taccccttca ccaccctgca tccgaacttg
      901 ggcgtggtac gcatcgacga aaaccacagc ttcgtgatgg ccgacatccc cggcctgatt
      961 gaaggcgcgg cagaaggcgc gggtttgggg catcgtttcc tcaaacactt atcgcgcacc
     1021 ggattattgc tgcacgtcgt cgatttggcg cccttcgacg aagccgtcga tcccgccgaa
     1081 gaagcccttg ccatcatcaa cgaattacgc aaatacgatg aagaactcta tggcaaaccg
     1141 cgctggctgg tgctgaacaa actcgacatg ctcgatgaag aagaagccca agaacgcacc
     1201 gtcaccttcc tcgaagccgt cggctgggat tacccgaaac cggacgaccg cttccaattc
     1261 gatatggaaa ccccgcgcct cttccaaatc agcgcgctaa cccaccaagg cacgcaggaa
     1321 ttggtacacc aaatcaacca atacctgacc gagaaaaaac gcttggaagc cgaaaaagca
     1381 gaagcggaga aagcggcggc aaatgttgaa attatcgaac agcagcctaa aacggatacg
     1441 ggcgtgttta agccggagtg aaaaaagaaa gggtttcaga cgatatttgt tctacaaaag
     1501 gttgtttgaa acaattttaa agcaagattg aaatggatta aaaatgtcat ctaaccaata
     1561 tatccagtcg cttgagttga caaactttac cgttcttcca aacgatactt ttgaattttc
     1621 tgaaaatctc aatgttattg ttgccgaaaa cggttgtggc aaaacacatt tgttaaaaat
     1681 tttatacagc ctgctagaag taacatctaa caccaaaaac agattactga aaacagaatt
     1741 gcaaaaaagt tttgccgata aattactgaa tgttttccgt cccgattcct tgggcaggtt
     1801 gagcaaacgt ttgcaggggc gcgggcgtac ggaaatcgtt ttaaaactgc aaaacggcac
     1861 tacgcatagt agtctgaatt tttccagtaa ctcatcatcg caagtcaatg ttcaaagcat
     1921 cggattgaaa gaaaatgaat atactccgac acctatcttc ttacccagcc gcgaattaat
     1981 tacactttgc ccttggttca cttctttgta tcaaaaccaa agcatccctt tcgaagaaac
     2041 ttggtttgat gcctgtatgc aattaaatca cccgcttgcc aaaggcccca gagaaacaaa
     2101 aattcgagaa ttgctcgaac ctattgagaa tgcgatgggc ggaaaagtat ctgaagaaca
     2161 aggaaggttt tatctatcgc tgacaaatac gggcggtaag attgaagcac ctttggtagc
     2221 agagggtttg cggaaatttg tcatgattgc ccggttaatc gcaacagggg cattgctgga
     2281 taaaggctat ctattttggg atgaaccgga agctaatctg aatcctaaat tgatcaaagt
     2341 ggctgcccgt attatttggt ctttatctca acagggtatt caggttttta ttgcaacaca
     2401 ctctttattc ttattgagag aattggaact tttgaagctt gaaaataaca cggatttacc
     2461 tgtacgtttt ttctcgttga ttgcttccga tgatggaaca aaagtggagc aagggaacag
     2521 tattaatgat ttgaacacat tgatattgtt agatgaaaac attatgcagg cagacaggta
     2581 tctgaatatt tgaggttaag ctatgactac aatcacagaa aagagattaa cctttgcttt
     2641 tcctgaagat tattacgtta ccaagtatga cgaatgggaa cattataaaa tttttcagaa
     2701 cagttgtaat ttacgtaata aaattgatac aaatgaaaag ggaaagaacg ggataaatca
     2761 aagtgtagat gacgataatg gcagctcagg tgtagatata atagcccttc atgagtcgac
     2821 actttggcta atcgaaatta aagattatta ccgacttggg cttgagccaa atgcccaatc
     2881 aatagatgaa aaactttctg acctaccata tttaatcgca agaaaaattc gagattcatt
     2941 ggcaggattg gtttctgcta aatttaaagc agaaaaacag gaagaaaaag atttttctcg
     3001 cttggcttta gattgtaacg aaataaaaat agtccttcat attgaaatgc ctagtataag
     3061 aagtaaactt tatccctcgt catcagattt agcaaactta ttaaaagata agtttaaatt
     3121 atcagaattt acaaaaaatt ttgcaaactg ttatgcagaa ccaattttca caaatatcag
     3181 ccatattaat aatccgcaac tacgtaatgt tccttggtcc gtatctacag gaacagaaca
     3241 gaaattatca tccgagcaac aaagattaat ccataatccc atgaccacca tctacaacac
     3301 ccttacccgc caaaaagaac cctttgcccc catcgaccct gaaaacgtgc gtatgtacgt
     3361 ttgcggcatg actgtttacg actattgcca cttaggccat gcccgtgtga tggtggtgtt
     3421 cgacatgatt gcccgttggt tgcgtgagtg cggttatccg ctcacttatg tgcgcaacat
     3481 caccgacatc gacgacaaaa tcatcgcccg tgcggctgaa aacggcgaaa ccatcggcga
     3541 actgaccgcg cgtttcattc aggctatgca cgaagatgcc gatgctttag gtgtgttgcg
     3601 tccggatatt gagccgaagg cgacggaaaa cattccgcaa atgattgcca tgattgaaac
     3661 cctgattcaa aacggcaagg catatcctgc cgcaaacggc gatgtttact acgccgtgcg
     3721 cgagttttcc gcttacggcc aattgtcggg caaatcgttg gacgacctgc gcgcaggcga
     3781 gcgcgtggag gtggacggtt tcaaacgcga tccgcttgat tttgtgttgt ggaaagcggc
     3841 caaagcaggc gagccggcat gggaaagccc gtggggcaat ggccgtccgg gttggcacat
     3901 tgaatgctcc gccatgagtg aaaacctgtt cggcgatact ttcgacatcc acggcggcgg
     3961 tgcggatttg cagtttccgc accacgaaaa tgaaatcgcc caaagcgtcg gtgccagcgg
     4021 tcatacctgc ggccacgacc acgcgcaaac ccatcacggt caaagcatcg ccagccacgt
     4081 caaatactgg ctgcacaacg gcttcatccg cgtggatggc gaaaaaatgt cgaaatcgct
     4141 gggcaacttc ttcaccatcc gcgaagtatt gaaacaatac gaccctgaag ttgtgcgttt
     4201 cttcatcctg cgcgcccact accgcagccc gttgaactat tccgacgcgc atttggacga
     4261 cgcgaaaggc gcgttgaccc gtctatacac caccttgaaa aacacgcctg ccgccgcgtt
     4321 tgatttgtct gaaaacgcca acgactacac ccgccgcttc tacgccgcca tgaacgacga
     4381 tttcggtacg gttgaagcgg ttgccgtgtt gtttgagctg gcaggcgaag tgaacaaaac
     4441 caatgacgca cagctcgccg gctgcctgaa agccttgggc ggcatcatcg gactgctgca
     4501 acgcgacccg accgagttcc tgcaaggcgg cgcggcttca gacggcctct ccaacgaaga
     4561 aatcgaagac ttgatcgccc ggcggaaaca ggcgcgcgcc gataaaaact gggcggaatc
     4621 cgaccgcatc cgcgaccttc tgaacgaaca caaaatcatt ttggaagaca acgccggcgg
     4681 cacgacttgg cggcgcggtt aaaatccatc gaaaagggta aatgtgccgc attttgccct
     4741 tttttgttca gacggcattt ttattgtgag aacttatttt tattatgctt aaaatcattt
     4801 ccgccaacgt caacggcatc cgctccgcct acaaaaaagg tttttacgaa tacatcgccg
     4861 catcgggcgc ggacattgtc tgcgtgcagg aactcaaagc gcaggaggct gatttgtctg
     4921 ccgatatgaa aaatccgcat gggatgcacg gccattggca ttgcgccgag aaacgcggtt
     4981 acagcggcgt ggcggtgtac agcaaacgca aacccgacaa tgtgcaaatc ggtatgggca
     5041 ttgaagaatt cgacagggaa gggcgatttg tgcgttgcga tttcgacagg ttgagcgtta
     5101 tttcgcttta cttgcccagc ggcagcagcg cggaagaacg ccagcaggtg aaataccgtt
     5161 tccttgatgc gttttaccct atgctcgaag cgatgaaaaa cgaaggacgc gacattgtcg
     5221 tttgcggcga ctggaacatc gcccaccaaa acatcgacct gaaaaactgg aaaggcaatc
     5281 agaaaaattc aggtttcctg ccggaagaac gcgagtggat aggcaaggtt atccacaagc
     5341 tcggttggac ggatatgtgg cgcacgcttt atcccgatgt gccgggctac acgtggtgga
     5401 gcaaccgcgg gcaggcgtat gcgaaagatg tcgggtggcg catcgattat cagatggtta
     5461 cgcccgaact tgccgccaaa gccgtgtccg cacacgttta taaagatgaa aaattttccg
     5521 accatgcgcc gctggtcatg gagtatgact atgctgccga ataaagtttt gggtaaatat
     5581 gattggaatg tggacggaaa aacaggaata ggcgcagcat gggttgtcgc tgcgtttatc
     5641 ctgcccatat tggtgtgggc gatatttatg ttgtcgcgga tgcaaggctg gcttgcgccg
     5701 accaaggcaa acccgacatg ggcgttggtg tggctgctga tctgcatgcc ctgcctgctg
     5761 attgcgtcca aatgtttggg ttggaaaggc tggcggcggg tggtgaatat ttttgcctgc
     5821 ctgaccgtct gcgccatatt gagcgtaccc gcttcgctgc tcatcgcctt taccctccgg
     5881 gacctgctga aataggcggg gcggtatgca gggttttgaa ctcgaagatt tgacgctttg
     5941 gctgatacgc gatgccggag aggatcagat gtggatagac cgttgggcga tcagttatcc
     6001 cgtcgtgcag atgtccgaag cgtcggccgg tcaaagcata ggggaatggc aggcagggct
     6061 tcggacggca tttgaacgca tacgtggcaa atacgtcgcc gttgtcgcac acggtgcggg
     6121 cgcggccgca tttttggcgt ggctgtatca ggcagacatc ctgacacgga agaaaattgc
     6181 caacatcatc cttgtaccgc agcgtcccga tatttttccc gacgatgcgg aacacacttt
     6241 ccaacgcgtc cgctgtccct gccgtgccgc attggtggtg tccgaacacg gggatgtgcc
     6301 gcgagattgg gcgcaaaaac aggcggatac gtggaacgcg cgcctgttgg tttccccgca
     6361 ttcgggcagt ttgaacggta tgctcggcgg ctggcagtgg ggtatgaagc tgatgcagga
     6421 aatgttgctg gcgtgaacgc ttgccttatc ggaatttcgg ctgtagcagg aagtccgcat
     6481 atcgggctgt tttcattgac gtgcaggcgc atcaggttcg gataaagtcg ggctgccgca
     6541 taccgccgga aatggaatcc tttatttttt acgtcggcag tgccggcagg gcgttatccg
     6601 gcagggttgc aaggaaaatg ccgtctgaaa caggcttcag acggcatttc cattcaaaca
     6661 gggcttacag gctgcccaac acgatacgca atacgcggcg cagcggctcg gccgcgcccc
     6721 acaagagctg gtcgccgacg gtgaacgcgc tgatgtattc gccgcccatg cccagtttgc
     6781 ggatgcgtcc gacagggacg gacagcgtgc cggtaacttt ggcaggagtc agctcgtgga
     6841 tgctggcttc tttttcattg gggatgactt tcacccagtc attcgcgcct gccaaaatcg
     6901 tttcgatttc ggaaacaggc aggtcttttt tcaacttcag agtgatggct tggctgtggc
     6961 agcgcatcgc gccgacgcgg acgcacaggc cgtcaatcac ggttggattg tcgctgcggc
     7021 cgaggatttt gttggtttcc acgccgcctt tccattcttc tttggactgg ccgttgccca
     7081 aatccacgtc aatccacgga atcaggctgc cggcgagcgg tacgccgaag ttggctttcg
     7141 gatagtcttc gctgcgcagg aaatcggata ctttgcggtc gatgtcgaga atcgagccgg
     7201 caggatcggc aagttcgtcc gccacttggg cgtgaaccgc gcccataccg ctgatgagtt
     7261 cgcgcatgtt tttcgcacct gcgccggaag cggcttggta ggtcatgctg gttgcccatt
     7321 cgaccaaatc gttttggaac aggccgccca aagccatcag catcagggag acggtgcagt
     7381 tgccgccgat gtagtttttc acgccgtttt tcaaaccgtt gtcgatgacg ttgcggttga
     7441 cagggtcgag gacgataatc gcgtcgtctt tcatacgcag cgaggatgcc gcgtcaatcc
     7501 agtaaccgtt ccagccgctg tcgcgcaggg cttggaagac ggatttggtg taatcgccgc
     7561 cttggcaggt aacgatgatg tccattttgg cgagttcggc aacattgttg gcatctaata
     7621 atgttttagc cgcctgaccg aaatcagggg ctgcgccgcc gacgttggaa gtggtaaaga
     7681 aaaacgcttc aggaatgtgg gcgaagtcgt tttcttcttt catacgctgc atcaaaaccg
     7741 aaccgaccat accgcgccag ccgacgaaac ctactttcat tgtgttgctc cttgaggaaa
     7801 aatcgt
//
LOCUS       DAWFHY010000091         7717 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.091, whole genome shotgun sequence.
ACCESSION   DAWFHY010000091 DAWFHY010000000
VERSION     DAWFHY010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7717
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.091"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(600..1355)
                     /locus_tag="WGQ68_001773"
     CDS             complement(600..1355)
                     /locus_tag="WGQ68_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEZ0715539.1"
                     /translation="MKFARLLFLCAALAGTQAAAAEMVQIEGGSYRPLYLKKDTGLIK
                     VKPFKLDKYPVTNAEFAEFVNSHPQWQKGRIGSKQAEPAYLKHWMKNGSRSYAPKAGD
                     LKQPVTNVSWFAANAYCAAQGKRLPTIDEWEFAGLASATQKNGSNEPGYNRTILDWYA
                     DGGRKGLHDVGKGRPNYWGVYDMHGLIWEWTEDFNSSLLSSGNANAQMFCSGASIGSS
                     DSSNYAAFLRYGIRTSLQSKYVLHNLGFRCTSR"
     gene            complement(1482..2642)
                     /gene="nirK"
                     /locus_tag="WGQ68_001774"
     CDS             complement(1482..2642)
                     /gene="nirK"
                     /locus_tag="WGQ68_001774"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase, copper-containing"
                     /protein_id="HEZ0715540.1"
                     /translation="MKRQALAAIIASMFALAACGGEPAAQTPAASAEAASSAAQTAAE
                     TPAGELPVIDAVTTHAPEVPPAIDRDYPAKVRVKMETVEKTMKMDDGVEYRYWTFDGD
                     VPGRMIRVREGDTVEVEFSNNPSSTVPHNVDFHAATGQGGGAAATFTAPGRTSTFSFK
                     ALQPGLYIYHCAVAPVGMHIANGMYGLILVEPKEGLPKVDKEFYIVQGDFYTKGKKGA
                     QGLQPFDMDKAVAEQPEYVVFNGHVGAIAGDNALKAKAGETVRMYVGNGGPNLVSSFH
                     VIGEIFDKVYVEGGKLINENVQSTIVPAGGSAIVEFKVDIPGSYTLVDHSIFRAFNKG
                     ALGQLKVEGAENPEIMTQKLSDTAYAGNGAASAASAPAASAPAASESEKSVY"
     gene            3012..5267
                     /locus_tag="WGQ68_001775"
     CDS             3012..5267
                     /locus_tag="WGQ68_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HEZ0715541.1"
                     /translation="MGQYKKLWYLLFAVLAVCFTILGYMGSEVYKKAPPYPEQVVSAS
                     GKVLMTKDDILAGQSAWQSTGGMEVGSILGHGAYQAPDWTADWLHRELSAWLDLTAQQ
                     TYGKKFDEVSPEEQAVLKTRLADEYRNQSRIKEDGSVVISDTRVKAIESILPYYHGVY
                     GDDPALQTTREHFAMKNNTLPSQEAREKLFDFFFWTSWSASTNRPDETFTYTNNWPHE
                     PLINNVPTTENYMWSFTSVVLLLMGIGLLMWGYSFLTKHEEVEVPSEDPISKIQLTPS
                     QKALGKYVFLTVALFVVQVLLGGLTAHYTVEGQGFYGIDEALGFEMSDWFPYALTRTW
                     HIQSAIFWIATGFLTAGLFLAPIVNGGKDPKFQRAGVNFLYIALFIVVGGSYAGNFFA
                     LTHILPPEFNFWFGHQGYEYLDLGRFWQLLLMVGLLLWLFLMLRCTVSAFKEKGVDKN
                     LLAIFVASMVGVGVFYAPGLFYGEKSPIAVMEYWRWWVVHLWVEGFFEVFATAAFAFV
                     FYNMGFVRRSTATASTLAAAAIFMLGGVPGTLHHLYFSGSTSASMAIGACFSALEVVP
                     LVLLGREAYEHWSYQHLSDWAKRLRWPLMCFVAVAFWNMIGAGVFGFLINPPISLFYI
                     QGLNTSAVHAHAALFGVYGFLALGFVLLVARYLKPNAQFDDKLMTWGFWLLNGGLVGM
                     IAISLLPVGAIQAYASITHGLWYARSEEFLQMEILDTLRWVRTAADLVFIGGAICVAI
                     QATKIVFGRDK"
     gene            5446..5979
                     /locus_tag="WGQ68_001776"
     CDS             5446..5979
                     /locus_tag="WGQ68_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEZ0715542.1"
                     /translation="MGIYDFQMKDAEGNAVDLSGYRGKVLLIVNTATRCGLTPQYEAL
                     QKLYAQYTAEGLEILDFPCNQFREQAPESSGEIAQVCMMKFGTKFKIFDKIEVNGANT
                     APLYAYLKSVKPQDKGNHLFKDFVLKLAALGEKRDEGDIKWNFTKFLVNRDGEVVERF
                     APSVTPEEIEADIRALL"
     gene            complement(6042..7226)
                     /locus_tag="WGQ68_001777"
     CDS             complement(6042..7226)
                     /locus_tag="WGQ68_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEZ0715543.1"
                     /translation="MTEPTSRRRFLKTCTAAAGAGLLQACGTSATSVPPLPSSHSVVK
                     ARTVPLQTPRRQSSDGNLLRVVASSGFAEDTNRVNTALTRLYNVGFTVTNQQAGSRRF
                     QRFAGTDTQRAADFQEVASGRVATPKVLMGLRGGYGAARILPHIDFASLGARMREHGT
                     LFFGFSDVCAVQLALLAKGNMMSFAGPMAYSDFGKPAPGAFTMDAFIKGATQNRLTVD
                     VPYIQRADVETEGTLWGGNLSVLASLAGTPYMPDIDGGILFLEDIGEQPYRIERMLNT
                     LYLSGILKKQRAIVFGNFRMEKIRDVYDSSYDFSAVANHVSRTAKIPVLTGFPFGHIA
                     DKITFPLGAHARIRMNGNSGYSVAFEGYPTLDASALTLDTLLPPPDLPIFPESGVADI
                     SE"
     gene            complement(7266..7670)
                     /locus_tag="WGQ68_001778"
     CDS             complement(7266..7670)
                     /locus_tag="WGQ68_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEZ0715544.1"
                     /translation="MTAQPCPICTAQDEDILLQTPNLRVIAIHNDSGSPAFCRVIWRK
                     HIAEMTDLSAAERGELMEMVYKVEAAMRQVFRPAKINLASLGNVVPHLHWHIIARFEN
                     DASFPAPIWANPVRKHGMTLPQDWTEQLKKLL"
BASE COUNT         1737 a         1860 c         2161 g         1959 t
ORIGIN      
        1 aggcgggaat ctagacctta gaacaacagc aatattcaaa gattatctga aagtccgaga
       61 ttctagattc ccactttcgt gggaatgacg ggatgtaggt tcgtgggaat gacgtggtgc
      121 aggtttccgt gcggatgaat tcatcattcc cgcgcaggcg ggaatctaga ctttagaaca
      181 acagcaatat tcaaagatta tctgaaagtc tgagattcta gattcccact ttcgtgggaa
      241 tgacgggatg taggttcgtg ggaatgacgt ggtgcaggtt tccgtgcgga tggattcgtc
      301 attcccgcgc aggcgggaat ctagacctta gaacaacagc aatattcaaa gattatctga
      361 aagtccgaga ttctagattc ccactttcgt gggaatgacg ggatgtaggt tcgtgggaat
      421 gacgaaaagt tgcgggaatg acggaaagtg gcgggaatga cggttcgggc attccttaaa
      481 tcacccgtgt atcgctgtaa atcttagaga tggcggaata tagcggatta acaaaaacca
      541 gtacggcaag gcgaggcaac gccgtactgg tttttgttaa tccactataa ttgaaggggt
      601 tatcggcttg tgcaacggaa gcccaagttg tgcaagacat atttggattg caggctggta
      661 cggatgccgt agcggaggaa ggcggcatag ttggacgagt cgctcgaccc gatagacgcg
      721 ccgctgcaaa acatttgcgc gttggcattg ccggaagaaa gcaggctgct gttgaaatct
      781 tccgtccatt cccaaatcag cccgtgcata tcataaacgc cccagtagtt cgggcggcct
      841 ttgccgacat cgtgcaggcc tttccgtccg ccgtcggcat accaatcgag aatagtgcgg
      901 ttgtagccgg gttcgtttga gccgtttttc tgcgtggcgg aagcaagtcc ggcaaattcc
      961 cattcgtcaa tggtcggcag gcgtttgcct tgtgcggcgc aataggcgtt ggcggcgaac
     1021 caggaaacat tggttaccgg ttgttttaaa tcgcccgcct tcggcgcata gctgcggctg
     1081 ccgtttttca tccaatgctt caggtaagcg ggttctgcct gtttggaacc gatcctgcct
     1141 ttttgccatt gggggtggct gttgacaaat tcggcaaact cggcattggt aacgggatat
     1201 ttatccagtt tgaacggttt gactttaatc agaccggtat cttttttcag ataaagcggg
     1261 cggtagctgc cgccttcgat ttgaaccatt tcggcagctg ccgcttgagt gccggcgagt
     1321 gccgcacaga gaaagagtaa ccgtgcaaac ttcatatcat gcctcctgac aggcagttaa
     1381 aatcaatctt ccgaaaggaa agattggttg ttaaaaaacc actgccgtgc gtaatgaggt
     1441 acggcggcag tggtttgtcc cgctaatgac gggtatccga tttaataaac gcttttttcg
     1501 gattcagagg ctgccggggc agaagctgcg ggagcggaag ctgcggaagc cgcaccgtta
     1561 ccggcgtaag cggtatcact caatttttga gtcatgattt cagggttttc cgcaccctct
     1621 actttcaatt gccccaacgc gcctttgttg aatgcgcgga agatagagtg gtcaaccaaa
     1681 gtgtagctgc ccgggatgtc gactttgaat tcgacgatgg cagagccgcc ggcaggaacg
     1741 atggtgcttt gtacgttttc gttaatcagt ttgccgcctt caacataaac tttgtcgaag
     1801 atttcgccga tgacgtggaa ggaagatacc aagttcggac cgccgttacc aacgtacata
     1861 cgtacagttt cgcctgcttt ggctttcagc gcgttatcgc cggcgatagc acctacgtga
     1921 ccgttgaata cgacgtattc aggctgttcg gcaacggctt tgtccatatc gaacggttgc
     1981 agaccttgcg cgcctttttt gcctttggtg tagaagtcgc cttggacgat gtagaactct
     2041 ttatccactt tcggcaggcc ttctttaggc tcgaccaaaa tcaggccgta cataccgttg
     2101 gcgatgtgca taccgaccgg cgcgacggcg cagtggtaga tgtacagacc cggttgcagg
     2161 gctttgaagc tgaatgtgga agtacggccc ggagcggtaa aggttgcggc tgcaccgccg
     2221 ccctgaccgg ttgcggcgtg gaagtcgacg ttgtgcggaa cggtagaaga aggattgttg
     2281 gaaaattcca cttcaaccgt atcgccttcg cgtacgcgga tcatgcgacc cggaacgtcg
     2341 ccgtcaaatg tccagtagcg gtattccaca ccgtcgtcca ttttcatggt tttttcgacg
     2401 gtttccattt tcacgcgtac tttggcaggg tagtcgcggt cgattgcagg aggtacttct
     2461 ggagcgtggg tggtaactgc atcgatgacg ggcagttcgc ctgcaggcgt ttcggcggcg
     2521 gtttgtgcgg cggagcttgc ggcttcggcg gaagcggcag gggtttgcgc ggcaggttcg
     2581 ccaccgcagg cggctaaagc aaacatagaa gcgataattg cggctaaggc ttggcgtttc
     2641 ataatgtttt ccttttgtaa gaaaagtagg gagattggtt aagaactggt gtgattatgc
     2701 gtttgcccca ttgggggcat tttgatttaa gtcaaataat tcataaaata tgaacttaaa
     2761 atgatacttc taatacttta tagctactaa aaattacaat aaataggctg atttgcttaa
     2821 gggcttcggc aaatcaggca aaggcagggt agttgccgac taaagaataa tgatagttat
     2881 tatcatatat gtatttgttt tatatgtgag tttctttgaa caaatgttta tttgcgcggt
     2941 aaaccgtgct acaatcttta acattcatat tttgtgaatt ttaatccact atattaaaag
     3001 gagctctcaa aatgggacag tacaagaagc tgtggtactt gctgtttgcc gttctggcgg
     3061 tatgctttac cattcttggc tatatgggca gcgaggttta taagaaagcc ccgccttacc
     3121 ccgaacaggt cgtttccgca tccggcaaag tcttgatgac gaaagacgat attttggcag
     3181 gtcagtctgc gtggcagagt accggcggca tggaagtcgg ttcgattttg ggtcacggcg
     3241 catatcaggc tccggactgg acggccgact ggctgcatcg tgagttgtcc gcctggttgg
     3301 atttgaccgc gcaacagact tacggcaaaa aattcgatga agtttcccct gaagaacaag
     3361 ccgttctgaa aacccgactg gctgacgaat accgcaacca aagccgtatt aaagaagacg
     3421 gcagcgtcgt catcagcgat acgcgcgtga aagccatcga aagcatcctg ccttattacc
     3481 acggtgttta cggcgatgat cccgcgctgc aaaccacccg cgaacacttt gcaatgaaaa
     3541 acaacacatt gccaagccaa gaagcgcgtg aaaaactgtt cgacttcttc ttctggactt
     3601 cttggtctgc ttcgaccaac cgtcctgacg agacgttcac ttataccaac aactggccgc
     3661 acgagccttt gatcaacaac gtaccgacca ctgaaaacta tatgtggtca ttcaccagcg
     3721 ttgttcttct tctgatgggc atcggtttgc tgatgtgggg ttattccttc ctgaccaaac
     3781 acgaggaagt ggaagtgccg tctgaagacc cgatttccaa aatccaactg accccttcgc
     3841 aaaaagcatt gggcaaatac gtcttcctga cggtcgccct gtttgtggta caagtattgc
     3901 tgggcggcct gaccgcgcac tacaccgtcg aaggtcaggg tttctacggc atcgatgagg
     3961 cgttgggctt tgaaatgtcc gactggtttc cctatgccct gacccgtact tggcacatcc
     4021 aatccgccat cttctggatt gcaaccggct ttttgacggc aggtttgttc ctcgcgccga
     4081 ttgtcaacgg cggcaaagat cccaagttcc aacgtgcagg cgtgaacttc ctctatatcg
     4141 ccctcttcat cgtggtcggc ggttcttacg cgggcaactt cttcgcgttg acgcacatcc
     4201 ttcccccgga atttaacttc tggttcggac accaaggtta cgaatacctc gatttgggac
     4261 gtttctggca actccttttg atggtcggtc tgctgttatg gctgttcctg atgttgcgtt
     4321 gcacggtttc cgcctttaaa gaaaaaggcg tggacaaaaa cctgttggct atcttcgtag
     4381 cttctatggt cggcgtgggc gtgttctacg cgccgggtct gttctacggt gaaaaatccc
     4441 cgattgccgt tatggaatac tggcgttggt gggtggttca cctgtgggta gaaggcttct
     4501 tcgaagtatt tgccactgcc gcctttgcct ttgtcttcta caatatgggc tttgtccgcc
     4561 gcagtaccgc cactgcctcc actttggccg ctgccgccat ctttatgttg ggcggcgttc
     4621 cgggtacgct gcaccacctg tacttctccg gctctacctc cgcctctatg gcaatcggtg
     4681 cctgcttctc cgctttggaa gtcgtgccgc tggtgttgct gggccgtgaa gcatacgaac
     4741 actggtctta ccaacacctg tccgactggg cgaaacgcct gcgttggccg ctgatgtgct
     4801 tcgtggcagt cgccttttgg aatatgatcg gtgccggcgt attcggcttc ctgatcaatc
     4861 cgccgatttc cctgttctat atccaaggtt tgaacacttc tgccgttcac gcgcacgccg
     4921 ccttgtttgg tgtgtacggt ttcttggcat tgggcttcgt attgctggtt gcccgttatt
     4981 tgaaaccgaa cgcgcagttt gacgacaaac tgatgacttg gggcttctgg ctgctcaacg
     5041 gcggcttggt cggtatgatt gccatcagcc tgctgcctgt cggcgcgatt caggcatacg
     5101 cctccatcac gcacggtctg tggtatgccc gcagcgaaga gttcctgcaa atggaaatcc
     5161 tcgatacctt gcgttgggta cgtacggcag ctgacttggt ctttatcggc ggtgcaatct
     5221 gcgttgccat ccaagccact aaaatcgtat tcggccgcga caaataagtt ttgcggtatg
     5281 aaatgagcaa ctgcacccgt tttcgggtgc agttttttat tgcggattgg gacgggaatg
     5341 gaaaaggatg tggatttttt attttaatcg gctatacaat tgctgctttc atgccgtctg
     5401 aagcacgttt cagatggcat cggtttacat cagataagga taaaaatggg tatttacgat
     5461 tttcaaatga aagatgcgga aggcaatgcg gttgatttgt caggctatcg cggcaaggtt
     5521 ctgctgatcg tcaacacggc aacgcgttgc ggtctgaccc cgcaatacga ggctttgcag
     5581 aagctgtatg cacaatatac cgcagaaggc ttggagattt tggattttcc gtgcaaccag
     5641 ttccgcgaac aggctcccga aagcagcgga gaaattgccc aagtgtgtat gatgaagttt
     5701 ggcacgaagt ttaagatttt cgacaaaatc gaagtcaatg gagcaaatac cgcgcccttg
     5761 tatgcctacc tgaaatccgt caaaccgcaa gataaaggca atcatctgtt taaagatttc
     5821 gtgttgaagc tcgcagcttt gggagagaag cgcgacgaag gcgacatcaa atggaacttt
     5881 accaaattcc tcgtcaaccg cgacggcgaa gtggtcgagc gttttgcgcc tagtgttacg
     5941 cccgaagaaa tcgaagccga tattcgggca ttgctgtaat ctgccgccgt cttgggaaat
     6001 gccgtctgaa ggcttcagac ggcatttgtc cgtttgcggg tttattccga aatatcggca
     6061 acaccgcttt cggggaagat gggcaaatcc ggcggtggga gcagggtatc caaagtcagg
     6121 gcggacgcat cgagtgtggg gtagccttca aacgcgaccg aataaccgct gtttccgttc
     6181 atacggattc gggcgtgcgc gcccagcggg aaagtgattt tgtcggcaat gtgtccgaac
     6241 gggaagcccg tcagcacggg gattttcgcc gtgcgcgaaa catggttggc aacggcggaa
     6301 aaatcataag acgaatcata gacatctcga attttttcca tacggaaatt gccgaacacg
     6361 atggcgcgct gtttcttcaa aatacccgaa agatacagcg tattgagcat acgttcgatg
     6421 cggtagggct gttcgccgat atcttcgagg aacaaaatgc cgccgtcgat gtcgggcata
     6481 taaggcgtac cggcgagcga ggcgaggacg cttaagttgc cgccccacaa cgtgccttcg
     6541 gtttcgacat cggcgcgttg gatataagga acatcaacgg tcaggcggtt ttgggttgcc
     6601 cctttgataa aggcatccat cgtaaacgca ccgggggcag gtttgccaaa atcgctataa
     6661 gccatcgggc cggcaaaact catcatattg ccttttgcca acaatgccag ttggacggcg
     6721 cacacatcgc tgaatccgaa aaacagcgtg ccgtgttcgc gcatccttgc gccgagcgaa
     6781 gcaaaatcga tatgcggcag aatccgcgcc gcaccgtaac cgccgcgcaa gcccatcagc
     6841 actttaggcg tggcgacgcg gccggaagcg acctcttgga aatcggcggc acgttgcgtg
     6901 tccgtgccgg caaaccgttg gaaacggcgg ctgcccgcct gttggttggt tacggtaaaa
     6961 ccgacattgt aaaggcgggt taaggctgtg ttgacgcggt tggtgtcttc ggcaaatcct
     7021 gacgaagcga caacgcgcag aaggttgccg tccgaacttt gacggcgtgg cgtttggaga
     7081 ggcacggttc gggctttcac aacggaatgg gaagagggaa ggggcggaac ggatgtggcg
     7141 gatgtgccgc aagcctgaag cagccccgcg ccggcagcgg cggtgcaggt tttcagaaaa
     7201 cggcggcggg aggtcggttc ggtcataatt tccctttcat acggtttcag acggcattgg
     7261 cgggcttaaa gcagcttttt aagctgttcc gtccaatctt gcggcagggt cataccgtgt
     7321 ttccggacgg ggtttgccca aatcggcgcg gggaaagacg catcgttttc aaagcgggcg
     7381 ataatatgcc aatgcaggtg cggcacgaca ttgcccaagc tggcgaggtt gatttttgcc
     7441 ggccggaaca cttggcgcat agcggcttcg actttgtaca ccatttccat caattcgccg
     7501 cgttccgctg ccgaaaggtc ggtcatttcg gcgatgtgct tacgccaaat gacgcggcag
     7561 aatgcaggcg aaccgctgtc gttatggatg gcgatgacgc ggaggttggg ggtttgcagc
     7621 aaaatatctt catcttgtgc cgtgcagatg gggcagggtt gggcggtcat ttttccttat
     7681 tcctgaaagt gtttaaaaaa cggtagggta atttctg
//
LOCUS       DAWFHY010000092         7447 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.092, whole genome shotgun sequence.
ACCESSION   DAWFHY010000092 DAWFHY010000000
VERSION     DAWFHY010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7447
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.092"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(205..2808)
                     /gene="pepN"
                     /locus_tag="WGQ68_001779"
     CDS             complement(205..2808)
                     /gene="pepN"
                     /locus_tag="WGQ68_001779"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEZ0715545.1"
                     /translation="MSKTVRYLKDYQTPAYRILETDLHFDIAEPQTVVKSRLTVEPQR
                     VGEPLVLDGSTKLLSVKINGVAADYVLEGETLTIADVPSERFTVEVETEILPAENKSL
                     MGLYASGGNLFTQCEPEGFRKITFYIDRPDVMSKFTTTIVADKKRYPVLLSNGNKIDG
                     GECSDGRHWVKWEDPFAKPSYLFALVAGDLAVTEDYFTTMSGRNVKIEFYTTEADKPK
                     VGFAVESLKNAMKWDETRFGLEYDLDIFMVVAVGDFNMGAMENKGLNIFNTKFVLADS
                     RTATDTDFEGIESVVGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSGDRASRA
                     VRRIENIRLLRQHQFPEDAGPTAHPVRPARYEEMNNFYTMTVYEKGAEVVRMYHTLLG
                     EEGFQKGMKLYFQRHDGQAVTCDDFRAAMADANGINLDQFALWYSQAGTPVLEAEGHL
                     KNNIFELTVKQTVPPTPDMADKQPMMIPVKIGLLNRNGEAAAFDYQGKRATEAVLLLT
                     EAEQTFQFEGVTEAVVPSLLRGFSAPVHLNYPYSDDDLLLLLAHDSDAFTRWEAAQTL
                     YRRAVAANLAALSDGIGLPKHEKLLAAVEKVISDDLLDNAFKALLLAVPSEAELWDGT
                     ENIDPLRYHQAREALLDTLAAHFLPKWHELNRQAAKQENQSYEYSPEAAGWRTLRNVC
                     RAFVLRADPAHIETVAEKYGEMAQNMTHEWGILSAVNGNESDTRNRLLAQFADKFSDD
                     ALVMDKYFALVGSSRRSDTLQQVQTALQHPKFSLENPNKARSLIGSFSRNVPHFHVED
                     GSGYRFIADKVIEIDRFNPQVAARLVQAFNLCNKLEPHRKNLVKQALQRIRAQEGLSK
                     DVGEIVGKILD"
     gene            complement(3020..3814)
                     /locus_tag="WGQ68_001780"
     CDS             complement(3020..3814)
                     /locus_tag="WGQ68_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEZ0715546.1"
                     /translation="MTTILAFDIETVPDVQCIRTLYDLPSSLPDDEVVLFAQQKRRAQ
                     TGGDFMQHHLHQIVAISCCMRWGQDKVHVGTIGEMDDGEEVVIAKFFELIEKHTPQLV
                     SWNGGGFDLPVLHYRSLLHGINAARYWDMGDGDFGDSRDFKWNNYISRYHQRHCDLMD
                     LLALYQPRANVPLDDMAKLCGFPGKLGMDGSKVWDAFHAGRLKEIRNYCETDAVNTYL
                     MYLRFCLVSGRFDADEYEMEIKRIRNYLSAQTEDKPHWSEFVQAWK"
     gene            complement(4563..5432)
                     /locus_tag="WGQ68_001781"
     CDS             complement(4563..5432)
                     /locus_tag="WGQ68_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A biosynthesis lauroyl acyltransferase"
                     /protein_id="HEZ0715547.1"
                     /translation="MKFIFFVLYVLQFLPFALLHKLADLTGLLAYRLVKPRRRVGEIN
                     LAKCFPEWSEEKRKTVLKQHFKHMAKLMLEYGLYWYAPAGRLKSLVRYRNKHYLDEAL
                     SAGEKVIILYPHFTAFEMAVYALNQDVPLISMYSHQKNKILDAQILKGRNRYDNVFLI
                     GRTEGLRALVKQFRKSSAPFLYLPDQDFGRNDSVFVDFFGIQTATITGLSRIAALANA
                     KVIPAIPVREADNTVTLHFYPAWESFPSEDAQADAQRMNRFIEERVREHPEQYFWLHK
                     RFKTRPEGSPDFY"
     gene            complement(5483..6019)
                     /gene="ruvC"
                     /locus_tag="WGQ68_001782"
     CDS             complement(5483..6019)
                     /gene="ruvC"
                     /locus_tag="WGQ68_001782"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEZ0715548.1"
                     /translation="MSATVRILGIDPGSRVTGFGVIDVRGRDHFYVASGCIKTPADAP
                     LADRIAVIVRHIGEVVAVYKPQQAAVEQVFVNVNPASTLMLGQARGAALAALVSHKLP
                     VSEYTALQVKQAVVGKGKAAKEQVQHMVVQMLGLSGTPQPDAADGLAVALTHALRNHG
                     LAAKLNPSGMQVKRGRFQ"
     gene            complement(6022..6261)
                     /locus_tag="WGQ68_001783"
     CDS             complement(6022..6261)
                     /locus_tag="WGQ68_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HEZ0715549.1"
                     /translation="MPHTLPDISQCIRQNLEQYFKDLNGTEPCGVYDMVLHQVEKPLL
                     VCVMEQCGGNQSKASVMLGLNRNTLRKKLIQHGLL"
     gene            complement(6291..7301)
                     /gene="dusB"
                     /locus_tag="WGQ68_001784"
     CDS             complement(6291..7301)
                     /gene="dusB"
                     /locus_tag="WGQ68_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEZ0715550.1"
                     /translation="MHIGGYFIDNPIALAPMAGITDKPFRRLCRDFGAGWAVCEMLTS
                     DPTLRNTRKTLHRSDFADEGGIVAVQIAGSDPQQMADAARYNVSLGAQLIDINMGCPA
                     KKVCNVQAGSALMQNEPLVAAILEAVVKAAGVPVTLKTRLGWHDDHKNLPVIAKIAED
                     CGIAALAVHGRTRTQMYKGEAAYDLIAETKCRLNIPVWVNGDITSPQKAQAVLKQTAA
                     DGIMIGRGAQGRPWFFRDLKHYAEHGVLPPALSLAECSAAILNHVRAMHAFYGETAGV
                     RIARKHIGWYIDEMPDGEQTRREINRLDSAAAQYDMLAGYLERLAEKTDSWACAYRPN
                     AF"
BASE COUNT         1673 a         1965 c         1946 g         1863 t
ORIGIN      
        1 acaaaaacca gtacagcgtt gcctcgcctt accgtactat ctgtactgtc tgcggcttcg
       61 tcgccttgtc ctgatttttg ttaatccact ataattaata tgactttgcg gccgttttgc
      121 cattgcgtaa taaaacgatg gggaagtgat gataaaacgt gtgtgtaact atatcagaca
      181 gcattgtttt tctgtttgac ggcctcaatc caaaattttg ccgacgattt cgcccacgtc
      241 tttcgacaat ccttcctgcg cccgaatgcg ctgcaatgct tgtttcacca agtttttgcg
      301 gtgcggctcg agcttgttgc agaggttgaa cgcctgcacc aggcgggcgg cgacctgcgg
      361 gttaaagcgg tcgatttcga taactttgtc ggcgataaag cggtagccgc tgccgtcttc
      421 tacgtggaaa tgcgggacgt tgcggctgaa gctgccgata agcgatcggg ctttgttggg
      481 attttcgagg ctgaacttcg gatgctgcaa ggcggtttga acctgttgca gggtgtcgct
      541 gcggcggctt gagccgacga gggcgaaata tttgtccatc accagcgcgt cgtctgaaaa
      601 cttgtcggca aactgcgcca gcaggcggtt gcgcgtatcg ctttcgttgc cgttgacggc
      661 ggacaggatg ccccattcgt gggtcatgtt ttgcgccatt tcgccgtatt tttcggcaac
      721 ggtttcgatg tgcgcggggt cggcgcgcag gacgaaggcg cggcagacgt tgcgcagcgt
      781 gcgccagccg gcggcttcgg ggctgtattc gtaactttgg ttttcctgct tcgccgcctg
      841 acggttcaat tcgtgccatt tcggcaggaa gtgggcggca agcgtgtcca acaaggcttc
      901 gcgcgcctga tggtagcgca acgggtcgat gttttccgtg ccgtcccaca gctcggcttc
      961 agacggcacg gccaaaagca gggctttgaa ggcgttgtcc aagaggtcgt ctgaaatgac
     1021 tttttcgacg gcggcaagca gtttttcgtg tttcggcaac ccgatgccgt ctgaaagcgc
     1081 ggcgaggttg gcggcgacgg cgcggcggta gagcgtttgg gcggcttccc agcgcgtgaa
     1141 ggcgtcgctg tcgtgggcga gcaggagcag caggtcgtcg tcgctgtacg gatagttcag
     1201 atgcactggc gcgctgaagc cgcgcagcag cgagggaacg acggcttcgg tcacgccttc
     1261 gaactggaag gtttgttcgg cttcggtcag cagcaatacg gcttcggtcg cgcgtttgcc
     1321 ctgatagtcg aatgccgccg cttcgccgtt gcggttcagc agcccgattt tgacgggaat
     1381 catcatcggc tgcttgtccg ccatatcggg cgtaggcggc acggtttgtt tgacggttaa
     1441 ctcgaaaata ttgtttttca gatggccttc ggcttccaaa acgggcgtgc ccgcctggct
     1501 gtaccacaag gcgaactggt cgagattgat gccgttcgcg tccgccatcg ccgcgcggaa
     1561 atcgtcgcag gtcacagcct gtccgtcgtg gcgttggaaa tagagcttca tgcctttttg
     1621 gaagccctct tcgccgagca aggtgtgata catccgcacc acttccgcgc ctttttcata
     1681 aacggtcatg gtgtagaaat tgttcatctc ctcatatcgg gcggggcgca ccggatgtgc
     1741 ggtcggacct gcgtcttcgg ggaactggtg ctggcgcaaa aggcggatgt tttcgatgcg
     1801 gcgcacggcg cggctggcgc ggtcgccgga aaattcttgg tcgcggaaca cggtcagccc
     1861 ttccttcagc gaaagctgga accagtctcg gcaggttacg cggttgcccg tccagttgtg
     1921 gaaatattcg tgtccgacca cggattcgat gccttcaaaa tcggtatcgg tggcggtgcg
     1981 gctgtcggca aggacgaact tggtgttaaa gatgttcaaa cccttgtttt ccatcgcacc
     2041 catattgaaa tcgcctacgg cgacgaccat gaaaatatcc aagtcgtatt ccaagccgaa
     2101 gcgcgtttcg tcccacttca tcgcgttttt caaggattcc acggcaaagc cgaccttggg
     2161 cttgtccgct tcggtggtgt aaaactcgat tttgacgttt ctgccgctca tggtggtgaa
     2221 atagtcttcc gtgaccgcca aatcgcccgc aaccaaagca aacagatagc tcggtttggc
     2281 aaacgggtct tcccatttca cccaatggcg gccgtctgaa cactcgccgc cgtcgatttt
     2341 gttgccgttg gagagcaaaa cgggatagcg ttttttgtcc gcaacgatgg tggtcgtgaa
     2401 tttggacatc acatccggac ggtcgatgta gaacgtgatt ttgcggaagc cctccggctc
     2461 gcattgggta aacagattgc caccggaagc atacagcccc atcagcgatt tgttttccgc
     2521 cggcaggatc tcggtttcca cttcgacggt gaagcgttcg gacggcacgt ctgcaatcgt
     2581 cagagtctcg ccttccaaca cataatccgc cgccacgccg ttgattttga cggacaagag
     2641 tttcgtcgaa ccgtccaaca ccagcggctc ccccaccctc tgcggctcga ccgtcaaacg
     2701 ggatttcacg acagtttgcg gttcggcaat atcaaaatgc aggtcggttt caaggatgcg
     2761 gtaggcaggc gtttggtaat ctttcagata acggacggtt ttgctcattt ttttctttca
     2821 atattgtttg gtttgactgg aaaaggcttc agacagcacg gatacagctt ttaaagcgga
     2881 ttaacaaaaa tcaggacaag gcgacgaagc cacagacagt acagatagta cgggctgtac
     2941 cggtttgaat ttaatccacc atatgccgtc tgaaaccgaa acggcggcac aagcgtaccg
     3001 ccttcggaca gcaggttttc tatttccacg cttggacaaa ttctgaccaa tgcggtttat
     3061 cttctgtttg ggcggaaaga tagtttctga tccgcttgat ttccatttcg tattcgtccg
     3121 cgtcgaatct gccgctgacc agacaaaaac gcagatacat caaatacgta ttcacggcat
     3181 cggtttcgca ataattgcgg atttctttca gcctgcccgc gtggaacgca tcccacacct
     3241 tgctgccgtc catacccagc ttgcccggaa aaccgcacag tttcgccata tcgtccagcg
     3301 gcacgtttgc cctcggctgg taaagcgcga gcaaatccat caaatcgcag tggcgttggt
     3361 gataacggct gatgtagttg ttccacttga aatcgcggct gtcgccgaaa tcgccgtcgc
     3421 ccatatccca atagcgcgcg gcgttgatgc cgtgtagcag cgaacggtaa tgcagaaccg
     3481 gcaggtcgaa accgccgccg ttccaactga ccagttgcgg cgtatgtttt tcaatcaact
     3541 cgaaaaattt ggcgataacc acttcctcgc cgtcatccat ctcgccgatt gtaccgacat
     3601 ggactttatc ctgcccccaa cgcatgcagc acgaaatcgc cacaatctga tgaagatgat
     3661 gctgcataaa atccccaccc gtctgagcac ggcgtttttg ctgggcaaac agcaccactt
     3721 catcgtcggg cagcgaggac ggcaagtcat acagcgtacg gatacactgc acatcgggta
     3781 cggtttcaat atcgaaagcc aaaatcgtgg tcatgacagc accttgtatt taaaacagac
     3841 ttgcgcctat tgtgtcatta aaaggcggat aaaaaaagag gcaacccccc acaggattgc
     3901 cccaatacct caaatcagag atttacgctt cacaaacaat acaggctttc gcctgcggct
     3961 ttgcccgcgt agctcaactc tacgccggca aacttttgtt tcaccgtttc cgatgaaacc
     4021 ccgaccaatc gcaagattga ccggaaaatc ctttcagacg gcatttcctg cctgtcgtgt
     4081 aattccatgt agcgaaatgt acgccatttt ctacgctttg ccaagcattt tttacaatat
     4141 aaatgtcaaa acattaattt tataaaattg ctgaaaatat taaatatatg aatttttatt
     4201 ttaaatattt taataaatat aaatttaatt ttgatttata tttaaattta agcataaaat
     4261 gtcaaatatt aaaggaaata tgaaaagcat atattaaata tttatttata acgctatgtt
     4321 tttaaagaaa attaatttta atatattaac tagattgtct gcagatattc atgggtttgc
     4381 ggtatttctt ccaaaacctg cttcgaattt tccgaccaag tcttaaaaat attgtttttg
     4441 agatacttaa atagcagcga ttatcaaatg aaatctgttc atataatcta ccatttcgca
     4501 tttaaaaaaa caatcaggag tttcgactcg aaacgcctga tatgttttgt aattttacgt
     4561 agtcagtaaa aatcggggct gccttccgga cgggttttga aacgcttgtg cagccaaaaa
     4621 tactgctcgg gatgttcgcg cacgcgttcc tcgataaaac ggttcatgcg ctgcgcgtcg
     4681 gcctgcgcat cttcactcgg aaaggattcc caagccgggt agaaatgcaa tgtaaccgta
     4741 ttgtccgcct cgcggacggg gatggcgggt atcacttttg catttgcaag cgcggcaatg
     4801 cggctcaatc cggtaatcgt tgccgtctga ataccgaaaa aatccacaaa aaccgaatcg
     4861 ttgcgtccga aatcctgatc gggcagatac agaaacggcg cgctgctttt gcggaactgt
     4921 ttgacgaggg cgcgcagccc ttcggtgcgc ccgatgagga agacgttgtc gtagcggttg
     4981 cggcctttca aaatctgtgc gtccaatatc ttgttttttt ggtgggaata catactgatc
     5041 agcggaacat cctgattgag cgcgtacacc gccatctcga acgcggtaaa gtgcggatac
     5101 aggatgatga ctttttcccc tgccgataat gcctcatcca gataatgttt gttgcggtaa
     5161 cgcaccagcg acttcaaacg tcctgcgggc gcataccagt ataaaccgta ctccaacatc
     5221 aacttcgcca tgtgtttgaa atgctgcttc aatacggttt tacgcttttc ctcactccat
     5281 tcgggaaaac attttgccaa attgatttcc ccaacccttc ggcggggttt gaccagacgg
     5341 taggcaagca aacccgtcag gtcggcaagt ttgtgcagca gcgcaaacgg cagaaactgc
     5401 aaaacataca gtacaaaaaa tataaatttc atctcgatac acattttctt ttcaggtggc
     5461 aaaatacaaa tgccgtctga aactattgaa acctgccgcg cttgacctgc atccccgaag
     5521 gattgagttt ggcggcaagc ccgtggttac gtaaggcgtg ggtcagcgcc accgccaaac
     5581 cgtcggcggc atccggctgg ggcgttcccg aaagtcccaa catctgcacc accatatgct
     5641 gcacctgttc ttttgccgcc ttacccttgc cgaccaccgc ctgtttgacc tgcaaggccg
     5701 tgtattccga aacgggcagc ttatggctga ccaatgccgc caatgccgcg cccctagcct
     5761 gacccagcat cagcgtcgat gccggattga cgttgacgaa cacctgttcc accgccgcct
     5821 gctgcggctt gtaaacggca acgacttcgc cgatgtgccg cacaatcacg gcaatcctgt
     5881 ctgccagagg cgcatcggca ggcgttttga tgcagcctga ggcaacgtaa aaatgatcgc
     5941 gccccctgac atcgatgaca ccgaaacccg ttacgcggct gcccgggtct atgcccaaga
     6001 tacggacggt tgccgacata ttcacagcaa accgtgttga atcagttttt tacgcaaggt
     6061 attgcggttc agccccagca tcacggatgc tttggactgg ttgccgccgc attgctccat
     6121 cacgcacacc agcagcggtt tttccacctg atgcaatacc atatcgtaca cgccgcaagg
     6181 ttcggtaccg ttcaggtctt tgaaatattg ttctaaattt tgtctgatgc attgggaaat
     6241 atcgggaagg gtatggggca tgattgcact ttcaaaggat aatcaagtgt tcagaaggca
     6301 tttgggcggt aggcgcacgc ccaactgtcg gttttttcgg caagtctttc aagataacct
     6361 gcaagcatgt cgtattgcgc cgccgcactg tccaagcggt tgatttcacg acgtgtctgt
     6421 tcgccgtcgg gcatttcgtc gatgtaccag cctatgtgtt tgcgtgcaat gcgcacaccg
     6481 gcggtctcac cataaaacgc gtgcatggcg cggacgtggt tcaaaatggc ggcactgcat
     6541 tctgccaaac tcaaggcagg cggtaaaaca ccgtgttcgg cgtaatgttt caaatcgcgg
     6601 aagaaccacg gtctgccttg cgcgccgcgc cctatcatga tgccgtctgc ggcggtttgt
     6661 ttaaggacgg cttgggcttt ttgcggcgaa gtaatgtcgc cgttgaccca aaccgggatg
     6721 ttcagacggc atttggtttc ggcaatcagg tcgtaagccg cttcgccttt gtacatttgc
     6781 gtgcgcgtgc gtccgtggac ggcaagggcg gcaatgccgc aatcttcggc gattttggcg
     6841 atgacgggca ggtttttgtg gtcgtcgtgc caacccaaac gggttttgag ggtaacgggt
     6901 acgcccgccg ccttgaccac cgcttccaaa atggcggcaa ccagcggctc gttctgcatc
     6961 agcgcgctac cggcttggac attgcagact tttttagcgg gacagcccat gttgatgtcg
     7021 ataagctgcg ccccaaggct gacgttgtaa cgcgcggcat ccgccatctg ctgcggatcg
     7081 cttccggcaa tctgcacggc aacaatgccg ccttcatcgg caaaatcgct gcggtgcaag
     7141 gtttttctag tatttctgag cgtcgggtcg ctggtcagca tttcgcacac cgcccaacct
     7201 gcgccaaaat ctcggcaaag tcggcggaac ggtttgtcgg taatgcccgc catcggcgca
     7261 agtgcgatgg ggttgtcgat aaaatagccg ccgatgtgca taatgggtcc gcgtttcaaa
     7321 aaagtgcgac attgtacatt ttttaagcag gatttccaat ctccggacgc gcccgcgatt
     7381 ggggcgggca ccgttttatg gcataatccg cacacagatt tcctgccccg ccactcacag
     7441 gcgggca
//
LOCUS       DAWFHY010000093         7396 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.093, whole genome shotgun sequence.
ACCESSION   DAWFHY010000093 DAWFHY010000000
VERSION     DAWFHY010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7396
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.093"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(151..573)
                     /locus_tag="WGQ68_001785"
     CDS             complement(151..573)
                     /locus_tag="WGQ68_001785"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010356394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEZ0715551.1"
                     /translation="MSIMEVEVVSSEQKIYSGEATFIVVPTVQGELGIYPRHEPIMSL
                     VRPGALRLTVPGEDKEVLVAVSGGVLEVQPDKVTVLADVAVRSAEMDQARAEEAKKAA
                     EAGISQAKDDKALAEAHKALAAAIAQLKTLDYIRSHKK"
     gene            complement(584..1981)
                     /gene="atpD"
                     /locus_tag="WGQ68_001786"
     CDS             complement(584..1981)
                     /gene="atpD"
                     /locus_tag="WGQ68_001786"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEZ0715552.1"
                     /translation="MSQGKIVQIIGAVVDVEFPRDMIPRVYDALKLDENGLTLEVQQL
                     LGDGVVRTIAMGSSDGLKRGMTVSNTGAPITVPVGKGTLGRIVDVLGTPVDEAGPIDT
                     DKSRAIHQAAPKFDELSSTTELLETGIKVIDLLCPFAKGGKVGLFGGAGVGKTVNMME
                     LINNIAKAHSGLSVFAGVGERTREGNDFYHEMKDSNVLDKVAMVYGQMNEPPGNRLRV
                     ALTGLTMAEYFRDEKDENGKGRDVLFFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTL
                     AEEMGRLQERITSTQTGSITSIQAVYVPADDLTDPSPATTFAHLDATVVLSRDIASLG
                     IYPAVDPLDSTSRQLDPMVLGQEHYDVARGVQSTLQKYKELRDIIAILGMDELSDEDK
                     LTVMRARKIQRFLSQPFHVAEVFTGSPGKYVALRDTIAGFKAILNGEYDHLPEQAFYM
                     VGSIEEAVEKAKTLN"
     gene            complement(2019..2894)
                     /gene="atpG"
                     /locus_tag="WGQ68_001787"
     CDS             complement(2019..2894)
                     /gene="atpG"
                     /locus_tag="WGQ68_001787"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEZ0715553.1"
                     /translation="MAVGKEILTKIRSVQNTQKITKAMQMVSTSKMRKTQERMRLARP
                     YAEKVRMVMSHLAQTNTDHGIPLLESHREIRRVGFILITSDKGLCGGLNANVLKKFLA
                     QVQEYRNQGIEEEIVCLGSKGLMACQSIGLNVVASAVNLGDTPKMEMLLGPLTELFQR
                     YEKHEIDRIHLVYSGFVNTMRQEPRMEVLLPIGENVIGDSAPKSPFSWEYRYEPTALA
                     VLEYLVRRYLESVVYQALSDNMASEQAARMVAMKAATDNAGNAIKELRLVYNKSRQAA
                     ITTELSEIVAGAAAV"
     gene            complement(2919..4466)
                     /locus_tag="WGQ68_001788"
     CDS             complement(2919..4466)
                     /locus_tag="WGQ68_001788"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013449548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEZ0715554.1"
                     /translation="MQLNPAEISDLIKAKIENLSVNAEVRTRGTVISVTDGIVRIYGL
                     SDAMQGEMLEFPGNTFGLAMNLERDSVGAVVLGEYEHIKEGDTVTCTGRILEVPVGRE
                     LVGRVVDALGRPIDGKGPINTTLTAPIEKIAPGVIARKSVDQPMQTGLKAIDSMVPVG
                     RGQRELIIGDRQTGKTAVALDAIVNQKGTGVICIYVAIGQKASSIANVVRKLEEHGAM
                     EHTIVVAATASEAAALQYIAPYSGCTMGEFFRDRGEDALIVYDDLSKQAVAYRQISLL
                     LRRPPGREAYPGDVFYLHSRLLERAARVNEHEVEKLTNGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETDLFNAGIRPAINAGISVSRVGGAAQTKVIKKLGGG
                     IRLALAQYRELAAFSQFASDLDEATRKQLEHGEVVTELMKQKQFSTLNTAEMALTLWA
                     INNGSYSDVPVAKALAFESEFLSFVRTQHPEVLEAVNASGAMSDESEKTLEAAMKSFK
                     SSYAYQA"
     gene            complement(4477..5010)
                     /locus_tag="WGQ68_001789"
     CDS             complement(4477..5010)
                     /locus_tag="WGQ68_001789"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEZ0715555.1"
                     /translation="MAEFATIARPYAKALFGLAQEKSQIESWLGGLEKLAAVVQEGKV
                     ASLIDRPETNASEKADILIDLVGLKDKELKNFVIVLAGQKRLSILPEVYAQYQDLTLS
                     FNHIKSAVIYSAYPLTDKQVGELAQMLNKRFDSELKISVEIEPELIGGIKVEVGDQVL
                     DLSVQGKLSALYTTMTN"
     gene            complement(5015..5485)
                     /locus_tag="WGQ68_001790"
     CDS             complement(5015..5485)
                     /locus_tag="WGQ68_001790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEZ0715556.1"
                     /translation="MNINATLFAQIIVFFGLVWFTMKFVWPPIAKALDERAAKVAEGL
                     AAAERGKSDFEQAEKKVAELLAEGRNQVSEMVANAEKRAAKIVEEAKEQASSEAARIA
                     AQAKADVEQELFRARESLREQVAVLAVKGAESILRSEVDASKHAKLLDTLKQEL"
     gene            complement(5556..5792)
                     /gene="atpE"
                     /locus_tag="WGQ68_001791"
     CDS             complement(5556..5792)
                     /gene="atpE"
                     /locus_tag="WGQ68_001791"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEZ0715557.1"
                     /translation="MGLIAIACGLIVALGALGASIGIAMVGSKYLESSARQPELIGPL
                     QTKLFLIAGLIDAAFLIGVAIALLFAFVNPFAGA"
     gene            complement(5849..6715)
                     /gene="atpB"
                     /locus_tag="WGQ68_001792"
     CDS             complement(5849..6715)
                     /gene="atpB"
                     /locus_tag="WGQ68_001792"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004464456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEZ0715558.1"
                     /translation="MAGETITAADYIKHHLQSLTSLSDVTQGQGLKNIADFSFINLDA
                     VFFAVLLGVIGSFLLWRGAKKATAGVPGRFQAAVEILFEFVDDMCKSIIHNEKSRKAV
                     APLGLTLFVWIFLMNAMDMLPVDLLPMVWQGITGNHHALLRVVPTADLNTTLALAVGV
                     LLICIYYNIKIKGLGGWFHELFSAPFGAKLAPANFLLNLVEFLSKTVSHGMRLFGNMY
                     AGELVFLLIALLGGAWAASGSVEVMDPILFVFHIIAGLAWAIFHILVITLQAFIFMAL
                     AFVYIGQAHDAH"
     gene            complement(6705..7058)
                     /locus_tag="WGQ68_001793"
     CDS             complement(6705..7058)
                     /locus_tag="WGQ68_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HEZ0715559.1"
                     /translation="MKQIIILQSAVLSICAAVAFAVWGFAGFLSAVGGGLSYLLPTFV
                     AVLLLKLFRGNPFLQSRMFVFGEILKVVLSLLSMLAVFAVWHQSLVFAPFLMGLLGVS
                     HLVFLVLLRVKDYGR"
BASE COUNT         1949 a         1967 c         1676 g         1804 t
ORIGIN      
        1 ctatatgttt cggttaggcg gcaggctgcc ctattgaata ccttaaagca ggctatgcct
       61 gccaacgcca tatccaaaca cagtctttaa tttaaatccg gaaaataaaa agcacgacca
      121 aacggtcgtg cttttccaaa ccaaacaagt ttatttcttg tgcgaacgga tatagtccaa
      181 agttttgagc tgtgcaatcg cagcagccaa tgctttatgt gcttccgcca aagccttatc
      241 gtctttagct tgggaaatgc ccgcttctgc ggcttttttc gcctcttccg cacgtgcctg
      301 atccatctcc gcactgcgga cggcaacatc cgccaagaca gttactttat caggctgtac
      361 ttccaaaaca ccgccggaaa cagcaaccaa aacctcttta tcctcgcccg gaacggtcaa
      421 acgcaaagcc cccggccgca ccaaactcat aatcggctcg tgtcgcggat aaataccgag
      481 ttcgccctgt acagtcggaa caacgataaa tgttgcctcg cctgaataga ttttctgctc
      541 gctacttacc acctcaactt ccatgatgct catgccgacc tcctcagttt aaggttttcg
      601 ctttctcaac cgcttcttcg atgctgccga ccatatagaa tgcctgctcg ggcagatggt
      661 cgtattcgcc gttcaagatg gctttgaagc cggcaatggt atcgcgcagg gcgacatatt
      721 tgcccggaga acctgtaaac acttcggcaa cgtggaacgg ttgggacagg aagcgttgga
      781 ttttacgcgc acgcattacg gtcagtttgt cttcgtcaga caattcgtcc atacccaaga
      841 tggcgatgat gtcgcgcaat tctttgtatt tctgcagggt ggactgtacg ccgcgcgcca
      901 cgtcgtagtg ttcttgaccc aagaccatcg gatccagttg gcgtgaagta gagtcaagag
      961 ggtcaactgc cgggtaaata cccaaagagg caatatcacg gctcaatacg acggttgcgt
     1021 ccaagtgggc gaacgttgtt gccggagacg ggtcggtcaa gtcgtccgca ggtacatata
     1081 cggcttggat ggaagtaata gaaccggttt gggtagaggt aatacgctcc tgcaaacgac
     1141 ccatttcttc agccaatgtc ggttggtagc ccactgcaga cggcatacgg cccaacaatg
     1201 cggatacttc ggtaccagct agagtgtaac ggtagatgtt gtccacgaag aacaatacgt
     1261 cgcggccttt gccgttttcg tctttttcgt cacggaagta ttccgccatg gtcaaaccgg
     1321 tcaatgcgac gcgcaaacgg ttgcccggag gttcgttcat ctgaccgtaa accattgcca
     1381 ctttatccaa tacgttggaa tctttcatct cgtggtagaa gtcgttacct tcgcgggtac
     1441 gctcacccac gcctgcgaac acggacaagc cgctgtgcgc tttggcgatg ttgttgatca
     1501 attccatcat gttcacggtt ttacccacac cggcaccgcc gaacagacct actttaccgc
     1561 ctttggcaaa cggacacagc aagtcaatca ctttaatgcc cgtttcgagc aattcggttg
     1621 tggaagacag ttcgtcaaac ttaggagcgg cttggtggat ggcacgactc ttgtcggtat
     1681 cgatcggacc tgcctcgtca acaggcgtac ccaatacgtc gacaatacga cccaatgtac
     1741 ctttacctac cggcacagta atgggcgcac cggtattgct cacagtcatg ccgcgtttca
     1801 aaccgtccga gctgcccatc gcaatggtac ggactacgcc gtcgcccaaa agctgttgga
     1861 cttccaaagt cagaccgttt tcgtctaatt tcaaagcgtc gtaaacgcgc ggaatcatgt
     1921 cgcgtggaaa ttccacgtca acaaccgcac cgataatttg tacgattttg ccttggctca
     1981 ttatcgtatc ctaatttctg tacaggattc agacggcatc agacagccgc cgcacctgct
     2041 acaatttctg acaattccgt ggtaatcgca gcttgacgcg atttgttata taccaaacgc
     2101 aactctttga tggcattgcc tgcattgtct gttgcagctt tcatggcaac catgcgggct
     2161 gcctgttcgg atgccatatt gtcgctcaac gcctgataaa ccacagactc taaatagcgg
     2221 cgaaccagat attccaacac tgcaagtgca gtcggttcgt agcggtattc ccagctgaac
     2281 ggtgatttgg gagctgaatc gccaatcacg ttctcaccga taggcagcaa tacttccatt
     2341 ctcggttctt gacgcatggt attgacaaaa cccgaataca ccagatggat tctgtcaatt
     2401 tcatgtttct cataccgttg gaagagttct gtcaaaggtc cgagcagcat ttccattttt
     2461 ggggtatcgc ccaaatttac ggcactggca accacattca gaccaatgct ctgacacgcc
     2521 atcagacctt tactgccaag gcatacgatt tcctcttcaa taccttgatt ccgatactct
     2581 tgaacttgtg ccaaaaactt tttcagcacg ttggcgttca aaccgccaca caaaccctta
     2641 tcagacgtaa tcaaaataaa accgacacgt ctaatctccc gatgagattc cagtaacgga
     2701 ataccatgat cggtattggt ttgcgcaaga tggctcatca ccatacgcac tttttcggca
     2761 tacggacgcg ccaaacgcat ccgttcctga gtcttccgca ttttagaggt tgacaccatc
     2821 tgcatcgctt tagtgatctt ttgggtattc tgaacactgc ggattttggt gagaatctct
     2881 tttcctactg ccatttcaga ctcctttcac ttcaagcctt atgcctgata ggcgtaagaa
     2941 gatttgaagg atttcatggc tgcttcaagc gttttctcgc tctcgtcgga cattgcacct
     3001 gaagcattga cggcttccaa aacttccgga tgttgggtac ggacaaagct caaaaattca
     3061 gattcaaaag ccagagcttt ggcaaccgga acatcagaat acgaaccgtt gttgattgcc
     3121 caaagggtca aagccatttc agccgtattc aacgtactga actgtttctg tttcatcagt
     3181 tcggttacga cttcgccatg ctccaattgt ttgcgcgtag cttcatccaa atcggatgca
     3241 aattgcgaga acgccgccaa ttcacgatat tgtgccaacg ccaaacggat accgccaccc
     3301 agctttttaa tcactttggt ttgtgcagca ccgcctacgc gggatacgga aataccggca
     3361 ttgattgcag gacggatacc ggcgttgaag aggtcggttt ccaagaaaat ctgaccgtcg
     3421 gtaatcgaaa tgacgttagt cggaacgaaa gcagatacgt cgcccgcttg ggtttcgata
     3481 atcggcaaag cagtcagaga accggttttg ccttttactt cgccgttggt caatttctcc
     3541 acttcgtgtt cattgacacg tgctgcacgt tccaacagac gggagtgcag gtagaacaca
     3601 tcgccgggat aggcttcgcg gccgggcgga cggcgcaaaa gcagggaaat ttgacggtaa
     3661 gccacagcct gtttggacaa atcgtcataa acaatcaagg catcttcgcc gcggtcgcgg
     3721 aagaattcac ccatcgtaca accggagtaa ggtgcgatat attgcaatgc cgccgcttca
     3781 gatgcagttg cagcaaccac aatcgtgtgt tccatcgcgc catgctcttc caatttgcgg
     3841 accacgttgg caatagaaga tgctttttga ccgatagcga catagataca gataacaccc
     3901 gtaccttttt ggttgacgat ggcatccagc gctacggccg ttttaccggt ttgacggtcg
     3961 ccaataatca actcacgctg accgcgaccg acaggaacca tagagtcaat cgccttcaga
     4021 ccggtttgca tcggctggtc aaccgatttg cgcgcaatca cgcccggtgc gattttttcg
     4081 ataggggcgg tcaaagttgt attaatcggg cctttgccgt cgataggccg acccaatgca
     4141 tcaacgacgc gtccgaccag ttcgcgtccg accggcactt ccaagatacg accggtacag
     4201 gtaaccgtgt cgccttcttt aatatgttcg tactcgccca acactacggc gccgacggag
     4261 tcgcgctcca ggttcatcgc caagccgaaa gtgttacccg ggaattcgag catctcacct
     4321 tgcattgcat ctgacaaacc atagatgcga acgataccgt cagttaccga aatcaccgta
     4381 ccacgggtac gcacttcggc atttacagac agattttcga tcttggcttt aatcaaatcg
     4441 ctaatttcag caggattaag ctgcatgaaa actctcctaa ttcgtcatag tcgtgtacaa
     4501 ggcactcaat ttgccttgta cagacaaatc caaaacctga tcacccactt caacttttat
     4561 gccgccaatc agctccggtt cgatttcgac agagattttc agctcgctgt cgaaacgctt
     4621 attcagcatt tgcgccaact cgccgacctg tttgtcggtc aacggatagg cactgtaaat
     4681 gacggcagat ttgatatggt tgaatgataa ggtcaagtct tgatattgag catatacttc
     4741 cggcaatatc gacaaacgtt tctgcccggc caagacgata acaaagtttt tcaactcctt
     4801 gtctttcaaa ccgaccaaat cgatgaggat atctgctttt tctgaagcat tcgtttcagg
     4861 acggtcaatc aatgaagcca ccttcccttc ctgaacaacc gccgcaagtt tttccagtcc
     4921 gcccaaccaa gactcaattt ggcttttttc ctgagccaga ccgaacaatg cctttgcata
     4981 aggtctggca atcgttgcga actctgccat aagattacag ctcctgtttc agggtatcga
     5041 gcagttttgc gtgtttggaa gcatcgactt cgctgcgcaa aatagattcg gcacctttga
     5101 cagccaacac ggcaacctgc tcgcgcaggg attcgcgtgc gcggaacaat tcctgctcca
     5161 catcggcctt tgcctgagct gcaatgcgcg ccgcctcgga agaagcctgt tctttggctt
     5221 cttcgacaat tttggcggca cgtttttcgg cgttggcaac catttcggaa acctgattac
     5281 gcccttctgc caagagttct gcaacctttt tttcagcctg ctcgaaatcg cttttaccac
     5341 gctcggcagc tgccaagcct tcggcgactt ttgcggcacg ctcatccaaa gcttttgcaa
     5401 tcggcggcca cacgaatttc atggtaaacc ataccaaacc gaaaaagacg atgatttgag
     5461 cgaataatgt tgcattgata ttcacgttac ttaaccttcg tattggggtt aatcaaacag
     5521 gctgcgcctg tacggaacgg acgaatccgt cctgattatg cacctgcaaa cgggttaacg
     5581 aaggcgaaca gcagtgcaat ggcgacacca atcaagaatg cggcatcaat caaaccggca
     5641 atcaggaaca gtttggtttg cagcggaccg atcagttcgg gctgacgggc agaagactcc
     5701 aaatatttag aaccgaccat tgcgataccg atagaggcac ccagtgcgcc caatgcaacg
     5761 atcaaaccac atgcgatagc aatcaaaccc attttaaact ccttaaagaa acaaaggtta
     5821 aactacaaaa acaaactact taggaaaatc agtgcgcatc atgtgcctgt ccgatataga
     5881 cgaacgccaa cgccatgaaa ataaacgcct gcagggtaat caccaaaata tggaaaatcg
     5941 cccatgccaa accggcaata atgtggaata caaacagaat cggatccatg acttcgacgc
     6001 tgccggaagc cgcccaagca ccgccaagca aggctatcaa caagaatacc aactcgcctg
     6061 catacatatt gccgaacaac cgcataccgt gggatacggt tttagaaaga aactcgacca
     6121 aattcagcag gaagttcgca ggtgcgagtt ttgcaccgaa cggcgcgctg aacaactcgt
     6181 gaaaccatcc gcccaatcct ttgattttga tgttgtaata gatacaaatc agcaacacgc
     6241 cgacagcgag tgccaaagtg gtgttcaaat cggcagtcgg tacgacgcgc aacagggcgt
     6301 gatggttgcc ggtaatgccc tgccatacca tcggcagcaa atcgaccggc agcatatcca
     6361 tcgcgttcat caggaaaatc cagacaaaca gcgtcagacc caacggcgcg acggcttttc
     6421 tagacttttc gttgtgaatg atgctcttac acatatcgtc cacaaactca aacaagattt
     6481 ccacggcggc ctggaaacgt ccgggaacgc ctgccgtcgc tttttttgca ccgcgccaca
     6541 acaggaagct gccgattacg cccaacagga cggcaaaaaa gacggcatca aggttaataa
     6601 acgaaaaatc agcaatgttt ttcagtccct gaccctgagt aacatccgac aaactggtca
     6661 agctctgcaa gtggtgcttg atgtagtcgg cagcggtaat ggtttcacct gccataatct
     6721 ttcactctca acaatactaa aaaaaccaaa tggctgacac cgagcagccc catcagaaac
     6781 ggggcgaaca ccagcgattg atgccataca gcaaatacgg cgagcatgga caacagcgac
     6841 agcactactt ttaaaatctc tccgaagacg aacatcctgc tttgcaggaa ggggtttccc
     6901 ctgaaaagtt ttaaaagtaa aactgcaaca aacgtgggaa gcaggtagga caaaccgcca
     6961 ccgaccgccg aaaggaatcc ggcaaaaccc catacggcaa aggcaactgc ggcgcatatg
     7021 gacaatacgg cggattgtag gatgataatc tgcttcataa agggaatgtt tccgcctcga
     7081 atttgaggcg cggctaatat aatttagaag ccttattacg tcaagcaata tgttaatctt
     7141 tgtgaaacaa cgtatcccaa tccgccgcgc tcgccgcctg aataacggcg acaggtgtca
     7201 ttctaacaca cattacatat aattacagga tattaaggag tttgtccgca atttctttac
     7261 acttttaatg ttcttacgtg atttgtttgc tttacgtgga aataataaaa aatcaacgcg
     7321 aaattgtagc agtttatcgg tcggattgtc ggcagtttgg ggaatttgct caataaataa
     7381 aaggtcgtct gaaaat
//
LOCUS       DAWFHY010000094         7201 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.094, whole genome shotgun sequence.
ACCESSION   DAWFHY010000094 DAWFHY010000000
VERSION     DAWFHY010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7201
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.094"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(60..144)
                     /locus_tag="WGQ68_001794"
     tRNA            complement(60..144)
                     /locus_tag="WGQ68_001794"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(108..110),aa:Leu,seq:caa)
     gene            420..1073
                     /locus_tag="WGQ68_001795"
     CDS             420..1073
                     /locus_tag="WGQ68_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HEZ0715560.1"
                     /translation="MFSVPRSFLPGVFVLAALAACKPQDNSAAQAASSSASASAAENA
                     AKPQTRGTDMRKEDIGGDFTLTDGEGKPFSLSDLKGKVVILSFGFTHCPDVCPTELLT
                     YSDTLKQLGGQAKDVKVVFVSIDPERDTPEIIGKYAKQFNPDFIGLTATGDQNLPVIK
                     QQYRVVSAKVNQKDDSENYLVDHSSGAYLIDKNGEVAIFSPYGSEPETIAADVRTLL"
     gene            1152..1805
                     /locus_tag="WGQ68_001796"
     CDS             1152..1805
                     /locus_tag="WGQ68_001796"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HEZ0715561.1"
                     /translation="MQDNALTIALSKGRIFEETLPLLAAAGIVPTEEPEKSRKLIIGT
                     NHENIRLVIVRATDVPTYVRYGAADFGIAGKDVLIEHGGTGLYRPLDLEIAKCRMMVA
                     VRKGFDYEAASQPGCRLKIATKYPEIAASHFAGKGVHVDIIKLYGSMELAPLVGLSDA
                     IVDLVSTGNTLKANGLEAVEHIVDISSRLVVNKAALKTKYALLEPIIQAFGGAVKAK"
     gene            1909..2808
                     /locus_tag="WGQ68_001797"
     CDS             1909..2808
                     /locus_tag="WGQ68_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HEZ0715562.1"
                     /translation="MVAKIKKFSDSTLSVLNNGERRFYVYCLTDLKKDKILYIGKGCG
                     NRIFEHEWVASRSQDPVSGEIIDRKLKAISKCKKLGRYIISYHLTEVEALAAESALIH
                     FVKSVLGKKLKNKIAGHGPGGISVEELDRRFGFSSLPLNEINPDGLILAIKIHNAFDL
                     DTDEELDYLFDNQDDANLKSRTLGNWVIGKDVASKVKYVIGVHTGLQNAVVSAYEVDG
                     FETMVEETKNGRKQSRYRFRTTSRSEEVLAKLGLQQKCLPELKFGSGGEKAYIRPKTE
                     TETEQENIQTTPNPKIKKEKTKS"
     gene            2805..4094
                     /gene="hisD"
                     /locus_tag="WGQ68_001798"
     CDS             2805..4094
                     /gene="hisD"
                     /locus_tag="WGQ68_001798"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HEZ0715563.1"
                     /translation="MKKLNTQSPDFQAGLKALLAFETAQNPETERIVADICADVQKRG
                     DAALIEYTNKFDQTNAKSIDDLILTQADLNAAFERIPNDVQTALQTAALRVESYHQRQ
                     KMESWSYTDEDGTLLGQQITPLDRVGIYVPGGKAAYPSSVIMNAMPAHVAGVKEIIMV
                     VPTPKGERNDIVLAAAYVAGVTKVFTVGGAQAVAALAYGTETIPQVDKITGPGNAFVA
                     AAKRRVFGVVGIDMVAGPSEILVIADGTTPADWVAMDLFSQAEHDEIAQAILIGTSQA
                     YLDEVEAAMDRLIETMPRRDIIEASLGNRGAMILAKDLDEACEIANYISPEHLELSVE
                     NPQEWAKKIRHAGAIFMGRYTGESLGDYCAGPNHVLPTSRTARFSSPLGTYDFQKRSS
                     LIQVSEQGAQKLGETASVLAHGESLTAHARAAEFRMK"
     gene            4140..5237
                     /locus_tag="WGQ68_001799"
     CDS             4140..5237
                     /locus_tag="WGQ68_001799"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HEZ0715564.1"
                     /translation="MKSVRSFIRDDIQAMSAYQIADVPPGFAKLDSMESPVHPFAGHE
                     TLLQEWQARLAAAPIHLYPNPSGSGLQEALRSAFDIPDCADIALGNGSDELIQFITML
                     TAKPGAAMLAAEPSFVMYRHNAALYGMDYVGVPLNGDFTLNLPAVLEAVRKHRPALTF
                     IAYPNNPTGVCFTRAEIEAVIEASDGIVVVDEAYGAFNGDSFLPQAGSIPNLIVMRTV
                     SKIGFAGLRIGYAAGCPEVIGELQKILPPYNMNQLSLTTAKLALQHYGIISANIDSLK
                     NERERMFAELGKICRLNTFPSQANFITIRVPDADLLFDTLKQNRILVKKLHGAHPLLE
                     HCLRITVGSPAQNDAVLNIIRQLYCQPTDFL"
     gene            5234..6151
                     /gene="hisB"
                     /locus_tag="WGQ68_001800"
     CDS             5234..6151
                     /gene="hisB"
                     /locus_tag="WGQ68_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HEZ0715565.1"
                     /translation="MNLTKTQRQLHNFLTLAQEAGSLSKLAKLCGYRTPVALYKLKQR
                     LEKQAEDPDARGIRPSLMAKLEKHTGKPKGWLDRKHRERTVPETAAESTGTAETQIAE
                     TASAAGCRSVTVNRNTCETQITVSINLDGSGKSRLDTGVPFLEHMIDQIARHGMIDID
                     ISCKGDLHIDDHHTAEDIGITLGQAIRQALGDKKGIRRYGHSYVPLDEALSRVVIDLS
                     GRPGLVYNIEFTRALIGRFDVDLFEEFFHGIVNHSMMTLHIDNLSGKNAHHQAETVFK
                     AFGRALRMAVEHDPRMAGQTPSTKGTLTA"
     gene            6246..7115
                     /locus_tag="WGQ68_001801"
     CDS             6246..7115
                     /locus_tag="WGQ68_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEZ0715566.1"
                     /translation="MSANEYTQIGWIGLGQMGLPMVTRLLDGGIEVGVYNRSPDKTAP
                     ISAKGAKVYGNTAELVRDYPVIFLMVSDYAAVCDILNGVRDGLAGKIIVNMSTISPTE
                     NLAVKELVESAGGQFAEAPVSGSVGPATNGTLLILFGGDETVLNPLQKVFSLVGKKTF
                     HFGEVGKGSGAKLVLNSLLGIFGEAYSEAMLMARQFGIDTDTIVEAIGGSAMDSPMFQ
                     TKKSLWANREFPPAFALKHASKDLNLAVKELEQAGNTLPAVETVAASYRKAVEAGYGE
                     QDVSGVYLKLAEH"
BASE COUNT         1880 a         2115 c         1721 g         1485 t
ORIGIN      
        1 gtcttatcta tttcattaaa cagcaataga caaaaaaata accgctctaa aagcggttgt
       61 ggtgcccagg gtcggactcg aaccgacaca ccttgcggcg ggggattttg agtcccctgc
      121 gtctaccaat ttcgccacct gggctggtga agaagtcgtc attataatgg cttttgaaat
      181 tctgtaaacc tttttttgaa attattttat ctgtttttat tttatttttg attttaaata
      241 gaatttttat tattttaatc ttactgttct ttccgctcca aagattctgt atgattcggc
      301 aattcctgcc gtgcagacaa cgtaaaaaaa tactacatta aatctgccaa acgcgttaag
      361 atggaaatat tcaaattccg tacgaatcag gttttgctat ttattcttgg gagattgtca
      421 tgttttccgt accgcgttcc tttttgccgg gcgttttcgt acttgccgcg cttgccgcct
      481 gcaaacctca agacaacagt gcggcgcaag ccgcttcttc aagtgcatcc gcgtcagctg
      541 cggaaaatgc ggcaaagccg caaacgcgcg gtacggatat gcgtaaggaa gacatcggcg
      601 gcgatttcac gctgaccgac ggcgaaggca agcctttcag cctgagcgat ttgaaaggca
      661 aggtcgtgat tctgtctttc ggctttacgc actgtcccga tgtctgcccg acagagcttt
      721 tgacgtacag cgacacgttg aagcagttgg gcgggcaggc taaggacgtg aaagtggtgt
      781 tcgtcagcat cgatccggaa cgcgacacgc ctgaaatcat cggcaagtat gccaaacagt
      841 tcaatccgga ctttatcggt ctgacggcaa cgggcgacca aaacctgccg gtcatcaagc
      901 agcaataccg cgtggtttct gccaaagtca atcaaaaaga cgacagcgaa aactatttgg
      961 tcgaccactc ttccggtgcg tatctcatcg acaaaaacgg tgaggttgcc attttctcgc
     1021 cttacggaag cgagccggaa acgattgctg ccgatgtaag gactctgctc tgataaaacc
     1081 gtatgccgtc tgcaccgtcg gcgcctattc agacggcatt attgtttcaa ccgacaaagg
     1141 acatccacac catgcaggat aatgctttga ccatcgcctt atccaagggg cgcatttttg
     1201 aggagacgct gccgctgctt gccgctgccg gcattgttcc gactgaagag cctgaaaaat
     1261 cgcgcaagct gattatcggg acgaaccatg aaaacatccg ccttgtcatt gtccgcgcaa
     1321 ccgatgtgcc gacttatgtc cgctacggcg cggcggactt cggcattgcg ggcaaagacg
     1381 tgctgatcga acacggcggc acggggcttt accggccttt ggatttggag attgccaagt
     1441 gccgcatgat ggttgctgtg cgtaaagggt ttgattacga agcagcttcg caacccggat
     1501 gccgtctgaa gattgccacg aagtatcctg aaatcgcggc atctcatttt gccggcaagg
     1561 gtgtccatgt ggacattatc aaactgtacg gctcgatgga acttgcgccg ctggtcggct
     1621 tgagcgatgc gattgtggac ttggtttcga cgggcaacac cttgaaggca aacggcttgg
     1681 aagcagtcga acacatcgtc gacatttcca gccgcctggt ggtcaacaag gctgctttga
     1741 aaacgaaata cgcgctgctg gagccgatta ttcaggcgtt cggcggcgca gtgaaggcga
     1801 agtaagcatc catttgaata aagatgcgtt ttcagacgac cctatccgtt cccgccgaca
     1861 ggtcgtctga aaatatcacc ggcagtaaac tgtataggag aagttaaaat ggttgcaaaa
     1921 ataaaaaaat tctcagattc aaccctttcc gttttgaata acggcgagcg tcggttttat
     1981 gtctattgtc tgaccgacct gaaaaaagac aaaatcctct acatcggcaa aggctgcggt
     2041 aatcgtatct tcgagcatga atgggttgct agtcgttcac aagacccagt ctccggcgag
     2101 attatcgatc ggaaactcaa agccatctcc aaatgcaaga aactcggtcg ctatatcatc
     2161 agctatcatc tgactgaagt cgaagcactc gccgccgaat ctgccttaat tcattttgtt
     2221 aaatctgtct tgggtaaaaa actcaaaaat aaaattgccg ggcatggtcc gggtggtatt
     2281 agcgtagaag aactagatcg ccgctttgga ttctcttctc tcccacttaa cgagattaac
     2341 cccgacgggc tgattctcgc catcaaaatc cacaatgctt tcgatttaga tactgacgaa
     2401 gaattagact accttttcga caaccaagac gatgccaacc tcaaatcgcg tacgttgggc
     2461 aactgggtta tcggtaaaga tgttgcttca aaagtgaaat acgttatcgg cgttcacacc
     2521 ggtctgcaaa acgctgttgt cagtgcatac gaagtggacg gttttgaaac aatggttgag
     2581 gaaaccaaaa acggtagaaa acaatcccgt taccgtttcc gcactacctc tcgtagcgaa
     2641 gaggtattag ccaaactcgg tctgcaacaa aaatgcctgc ccgaattgaa gtttggtagc
     2701 gggggagaaa aagcgtatat cagacccaaa acagagacag aaactgaaca agagaatatt
     2761 cagacgaccc ccaatccaaa aataaaaaag gaaaaaacca aatcatgaaa aaactcaaca
     2821 cccaatcgcc cgatttccaa gccggactca aagccctgct ggcttttgaa accgcgcaaa
     2881 accccgaaac cgaacgcatc gtcgccgaca tttgcgccga cgtgcaaaag cgcggcgatg
     2941 cggctttgat tgaatacacc aacaaattcg atcagacaaa cgctaaaagc atcgatgatt
     3001 taatacttac gcaagccgat ttgaacgcgg cgttcgagcg cattccgaac gacgttcaga
     3061 cggcattgca gaccgccgcc ctccgtgtcg aaagctacca ccaacgccaa aaaatggaat
     3121 cgtggagcta caccgatgaa gacggcacgc tgttgggaca acagattacc ccgcttgacc
     3181 gcgtcggcat ttacgtcccc ggcggcaagg cggcatatcc gagttccgtc atcatgaacg
     3241 ccatgcccgc ccacgtcgca ggcgtgaaag aaattattat ggtcgtgccg acaccgaaag
     3301 gcgaacgcaa cgacatcgtg cttgccgccg catacgtcgc cggcgtaacc aaagtcttca
     3361 ccgtcggcgg cgcgcaggcg gttgccgccc tcgcctacgg cacggaaacc atcccccaag
     3421 tcgataaaat caccggtccg ggcaacgcct tcgtcgccgc cgccaaacgc cgcgtgttcg
     3481 gcgtggtcgg catcgacatg gtggcggggc cgtctgaaat cttggtcatc gccgacggca
     3541 cgacacctgc cgattgggtg gcgatggatt tgttcagcca ggccgaacac gacgaaattg
     3601 cccaagccat cttaatcggc acgtcgcaag cgtatctcga cgaagtagaa gccgctatgg
     3661 acaggctcat cgaaaccatg ccgcgccgcg acatcatcga agcctcgctc ggcaacaggg
     3721 gcgcgatgat actcgccaaa gacttggacg aagcctgcga aatcgccaac tacatttccc
     3781 ccgaacactt ggaattgtca gtcgaaaatc cgcaggaatg ggcgaaaaaa atccgccacg
     3841 ccggcgcgat tttcatggga cgctacaccg gcgaaagcct cggcgactac tgcgccggcc
     3901 caaaccatgt gttgcccacc agccgaaccg cccgcttttc ctcgcctttg gggacatatg
     3961 atttccaaaa acgctccagc ctgattcagg tttcggaaca gggcgcgcaa aaattaggcg
     4021 aaaccgccag cgtgctggca cacggcgaaa gcctgaccgc ccacgcccgc gcggcagagt
     4081 tccgtatgaa ataatgccga aacggcgtac aggcatattc caaccattaa ggaaacacga
     4141 tgaaatccgt ccgctccttc atccgcgacg acatacaagc tatgtcggca tatcagattg
     4201 ccgacgttcc gcccggcttt gccaaactcg attcgatgga aagtcccgtc cacccttttg
     4261 ccggacatga aacgctgttg caggaatggc aggcacggct tgccgccgcg cccatccatc
     4321 tttaccccaa tccctccggc agcggtttac aggaagcatt acgttcggcg ttcgacattc
     4381 ccgactgcgc cgacatcgcg ctgggcaacg gctcggacga gctgatacag ttcatcacga
     4441 tgctgaccgc caaaccgggc gcggcaatgt tggcagccga acccagtttc gtcatgtacc
     4501 gccacaacgc cgcgctgtac ggcatggatt atgtcggcgt tccactgaac ggagatttca
     4561 ccctcaacct gcccgccgtc ctcgaagccg tcaggaagca ccgccctgcc ctgaccttta
     4621 tcgcctaccc caacaacccc accggcgtat gtttcacgcg tgccgaaatc gaagccgtca
     4681 tcgaagcttc agacggcatc gtcgtcgtcg acgaagccta cggcgcattc aacggcgaca
     4741 gcttcctgcc gcaggcgggc agcattccca acctcatcgt catgcgtacc gtcagcaaaa
     4801 tcggttttgc cggactgcgt atcggctatg cggcaggctg tcccgaagtc atcggcgaac
     4861 tgcaaaaaat cctgccgccc tacaatatga accaactgag cctgaccacc gccaaactcg
     4921 ccctgcaaca ctacggcatc atctctgcca acatcgacag cctgaaaaac gaacgcgaac
     4981 ggatgttcgc cgaattgggc aaaatatgcc gtctgaacac ctttccaagt caggcaaact
     5041 tcattaccat acgcgtaccc gatgccgatt tgttgtttga cacgctcaaa caaaaccgca
     5101 tcttggttaa aaaactgcat ggcgcgcacc cgcttttgga acactgcctg cgcattaccg
     5161 taggcagccc cgcacaaaac gatgccgttc tcaacatcat tcgccaactt tactgccaac
     5221 caacggattt cctatgaatt tgactaaaac acaacgccaa ctgcacaact ttctgaccct
     5281 cgcccaagaa gcaggttcgc tgtccaagct cgccaaactc tgcggctacc gtacccccgt
     5341 cgcactctac aaactcaaac aacgccttga aaagcaggca gaagacccag atgcacgcgg
     5401 catccgtccc agcctgatgg caaaactcga aaaacacacc ggcaaaccca aaggctggct
     5461 cgacagaaaa caccgcgaac gcactgtccc cgaaaccgcc gcagaaagca ccggaactgc
     5521 cgaaacccaa attgccgaaa ccgcatctgc tgccggctgc cgcagcgtta ccgtcaaccg
     5581 caatacctgc gaaacccaaa tcaccgtctc catcaacctc gacggcagcg gcaaaagcag
     5641 gctggatacc ggcgtaccct tcctcgaaca catgatcgat caaatcgccc gccacggcat
     5701 gattgacatc gacatcagct gcaaaggcga cctgcacatc gacgaccacc acaccgccga
     5761 agacatcggc atcacactcg gacaagcaat ccggcaggca ctcggcgaca aaaaaggcat
     5821 ccgccgttac ggacattcct acgtcccgct cgacgaagcc ctcagccgcg tcgtcatcga
     5881 cctttccggc cgccccggac tcgtgtacaa catcgaattt acccgcgcac taatcggacg
     5941 tttcgatgtc gatttgtttg aagaattttt ccacggcatc gtcaaccaca gtatgatgac
     6001 cctgcacatc gacaacctca gcggcaaaaa cgcccaccat caggcggaaa ccgtattcaa
     6061 agccttcggg cgcgccctgc gtatggcagt cgaacacgac ccgcgcatgg caggacagac
     6121 cccctcgacc aaaggcacgc tgaccgcata aaaaaccata ccgtctgaaa cacccgcagg
     6181 cttttcagac ggtatcggaa cagataagat tacactacac tacaaacaga aaaggagtaa
     6241 acatcatgtc cgcaaacgaa tacacacaaa tcggctggat aggcttaggg caaatgggtc
     6301 tgcctatggt aacgcggctc ttggacggcg gcatcgaagt cggcgtatac aaccgctcgc
     6361 ccgacaaaac tgcccccatc tccgccaaag gcgcaaaagt ttacggcaac accgccgaac
     6421 tcgtccgcga ctatcccgtc attttcctga tggtttccga ctatgccgcc gtgtgcgaca
     6481 tcctgaacgg agtccgcgac ggattggccg gcaaaatcat cgtcaacatg agcaccatct
     6541 ccccgaccga aaaccttgcc gtcaaagaac ttgtcgaatc tgccggcgga caatttgccg
     6601 aagccccagt ttctggttcg gtcggtccgg ccaccaatgg cacgctgctc attctgttcg
     6661 gcggagacga aactgtttta aacccgttgc agaaagtgtt ttcccttgtc ggcaaaaaaa
     6721 ctttccactt cggcgaagtg ggcaaaggct cgggcgcgaa actcgtctta aactcgctct
     6781 taggcatttt cggcgaagcg tacagcgaag cgatgctgat ggcgcggcag ttcggcatcg
     6841 ataccgacac catcgtcgaa gccatcggcg gctcggcaat ggactcgccc atgttccaaa
     6901 ccaaaaaatc cctgtgggca aaccgcgaat tcccgcccgc cttcgccctc aaacacgcct
     6961 ccaaagacct caacctcgcc gtcaaagagc ttgaacaggc aggcaacacc ctgcccgccg
     7021 tcgaaaccgt tgctgccagc taccgcaaag cagtcgaagc cggctacggc gaacaggacg
     7081 tttccggcgt ttacctgaaa ctggcagaac actgacttcc ctttccaaac aatatgccgt
     7141 ctgaaatatt ttcagacggc atttttatcg ccccacgctt aaaatcagtc ccgattatga
     7201 c
//
LOCUS       DAWFHY010000095         7161 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.095, whole genome shotgun sequence.
ACCESSION   DAWFHY010000095 DAWFHY010000000
VERSION     DAWFHY010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 7161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7161
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.095"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="WGQ68_001802"
     CDS             complement(76..1422)
                     /gene="mnmE"
                     /locus_tag="WGQ68_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEZ0715567.1"
                     /translation="MSDNVPTIAAVATASGRGGVGVIRISGKNLLPMAEALCGKTPKP
                     RVATYADFTDADGQAIDSGLLLFFAAPASFTGEDVIELQGHGGPVVMDMLLNRCLELG
                     ARLAEPGEFTKRAFLNDKLDLAQAEGVADLIDASSRSAARLALRSLKGDFSRRIHGLV
                     EDLITLRMLVEATLDFPEEDIDFLEAADARGKLDGLRRAVDDVLANAQQGAILREGLN
                     VVLVGAPNVGKSSLLNALAGDEVAIVTDIAGTTRDAVRERILIDGVPVHIVDTAGLRE
                     TDDVVERIGIERSRKAVSEADVALVLVDPREGLNEKTRAILDALPPELKRIEIHSKSD
                     LHAHAAGGFGTGAETVIALSAKTGDGLDALKRTLLREAGWQGESEGLFLARTRHVNAL
                     KAAQEELSLAALCGNHQIELFAEHLRLAQVACGEITGEFTADDLLGVIFSRFCIGK"
     gene            1553..1708
                     /locus_tag="WGQ68_001803"
     CDS             1553..1708
                     /locus_tag="WGQ68_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715568.1"
                     /translation="MEKRNTYALLLGIGSLLGLFHPAKTAIRPNPADDLKNIGGDFQR
                     AIEKARK"
     gene            1705..2136
                     /locus_tag="WGQ68_001804"
     CDS             1705..2136
                     /locus_tag="WGQ68_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HEZ0715569.1"
                     /translation="MTENAQDKARQAVETVVKSPELVEQILSDEYVQIMIARRFHSGP
                     LPPPSDLAQYNDIISNGADRIMAMAEKEQAVRHETIRQDQTFNRRGQLYGFISVILIL
                     LFAVFLVWSGYPATAASLAGGTVVALAGAFVIGRSRDQGKN"
     gene            2270..6634
                     /gene="app"
                     /locus_tag="WGQ68_001805"
     CDS             2270..6634
                     /gene="app"
                     /locus_tag="WGQ68_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_168458961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesion and penetration autotransporter App"
                     /protein_id="HEZ0715570.1"
                     /translation="MKTTDKRTTETHRKAPKTGRIRFSPAYLAICLSFGILPQAWAGH
                     TYFGINYQYYRDFAENKGKFAVGAKDIEVYNKKGELVGKSMTKAPMIDFSVVSRNGVA
                     ALVGDQYIVSVAHNGGYNNVDFGAEGSNPDQHRFSYQIVKRNNYKAGTNGHPYGGDYH
                     MPRLHKFVTDAEPVEMTSYMDGRKYIDQNNYPDRVRIGAGRQYWRSDEDEPNNRESSY
                     HIASAYSWLVGGNTFAQNGSGGGTVNLGSEKIKHSPYGFLPTGGSFGDSGSPMFIYDA
                     QKQKWLINGVLQTGNPYIGKSNGFQLVRKDWFYDEIFAGDTHSVFYEPRQNGKYSFHD
                     NNNGTGKIDAKHEHNSLPNRLKTRTVQLFNVSLSETAQEPVYHAAGGVNSYRPRLNNG
                     ENISFIDEGKGELILTSNINQGAGGLYFQGDFTVSPENNETWQGAGVHISEDSTVTWK
                     VNGVANDRLSKIGKGTLHVQAKGENQGSISVGDGKVILDQQADENNKKQAFSEIGLVS
                     GRGTVQLNADNQFNPDKLYFGFRGGRLDLNGHSLSFHRIQNTDEGAMIVNHNQDKEST
                     VTITGNKDIATTGNNNSLDSKKEIAYNGWFGEKDTTKTNGRLNLVYQPAAEDRTLLLS
                     GGTNLNGNITQTNGKLFFSGRPTPHAYNHLNDHWSQKEGIPRGEIVWDNDWINRTFKA
                     ENFQIKGGQAVVSRNVAKVKGDWHLSNHAQAVFGVAPHQSHTICTRSDWTGLTNCVEK
                     TITDDKVIASLTNTDISGNVSLADHAHLNLTGLATLNGNLSANGDTRYTVSHNATQNG
                     DLSLVGNAQATFNQATLNGNTSASGNASFNLSDHAVQNGSLTLSGNAKANVSHSALNG
                     NVSLADKAVFHFESSRFTGQISGSKDTALHLKDSEWTLPSGTELGNLNLDNATITLNS
                     AYRHDAAGAQTGSATDAPRRRSRRSLLSVTPPASAESHFNTLTVNGKLNGQGTFRFMS
                     ELFGYRSDKLKLAESSEGTYTLAVNNTGNEPASLEQLTVVEGKDNKPLSENLNFTLQN
                     EHVDAGAWRYQLIRKDGEFRLHNPVKEQELSDKLGKAEAKKQAEKDNAQSLDALIAAG
                     RDAVEKTESVAEPARQAGGENVGIMQAEEEKKRVQADKDTALAKQREAETRPATTAFP
                     RARRARRDLPQPQPQPQPQPQRDLISRYTNSGLSEFSATLNSVFAVQDELDRVFAEDR
                     RNAVWTSGIRDTKHYRSQDFRAYRQQTDLRQIGMQKNLGSGRVGILFSHNRTENTFDD
                     GIGNSARLAHGAVFGQYGIDRFDIGISAGAGFSSGNLSDGIGGKIRRRVLHYGIQARY
                     RAGFGGFGIEPHIGATRYFVQKADYRYENVNIATPGLAFNRYRAGIKADYSFKPAQHI
                     SITPYLSLSYTDAASGKVRTRVNTAVLAQDFGKTRSAEWGVNAEIKGFTLSLHAAAAK
                     GPQLEAQHSAGIKLGYRW"
     gene            complement(6743..>7161)
                     /locus_tag="WGQ68_001806"
     CDS             complement(6743..>7161)
                     /locus_tag="WGQ68_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0715571.1"
                     /translation="DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEA
                     AVRFMLYCMRQQVFWDGNKRTATLFANGLMMAGGCGILEISEMQMPQFNEKLSAFYRS
                     GDDTDISKFVYQNCISGIDYFGTDEDIDFPDPPNMG"
BASE COUNT         1913 a         2057 c         1743 g         1448 t
ORIGIN      
        1 caaacggatc ggattcccgt ttttatggga taacggaatg ttcagccgga caccgccacg
       61 atgctttcga tccgtttatt ttccgataca aaacctcgaa aaaatcacgc cgagcaggtc
      121 gtccgccgta aactcgcccg tgatttcgcc gcatgcgacc tgcgccaagc gcaagtgttc
      181 ggcaaacagc tcgatttgat ggttgccgca caatgccgcc agcgacaatt cttcctgcgc
      241 tgctttgagt gcgttgacgt gccgcgtccg cgccaaaaac agcccttcgc tttcgccctg
      301 ccaaccggcc tcacgcaaca acgtccgttt cagcgcgtcc aagccgtcgc cggttttcgc
      361 cgacagcgcg atgacggttt ccgcgcccgt accgaacccg cctgccgcgt gtgcgtgcaa
      421 atcggatttg ctgtggattt cgatgcgttt caactccggc ggcaacgcgt ccaaaatcgc
      481 ccgtgtcttt tcattcaaac cctcgcgcgg atcgaccaac accagcgcga catcggcttc
      541 ggatacggct ttgcggctgc gttcgatgcc gatacgctcg accacgtcgt ccgtctcgcg
      601 caaacctgcc gtatcgacaa tatgcaccgg cacgccgtca atcaggatac gttccctgac
      661 cgcgtcgcgc gtcgttccgg caatatcggt aacaatcgcc acttcgtcgc ccgccaacgc
      721 gttcagcagg ctggacttgc ccacattcgg cgcgccgacc aatacgacat tcagaccttc
      781 gcgcaaaatc gcgccctgct gcgcgttggc aagcacatca tccacggcgc ggcgcaagcc
      841 gtccagtttg ccgcgtgcgt ctgccgcttc gagaaaatcg atgtcttcct cgggaaaatc
      901 cagcgtcgct tcgaccagca tccgtaaggt aatcaagtct tcgaccagac cgtgtatccg
      961 ccgcgaaaaa tcgcccttga gcgagcgcaa cgccagacgc gccgccgaac ggctggatgc
     1021 gtcaatcaaa tccgccacgc cttccgcctg tgccaagtcc agtttgtcgt ttaaaaacgc
     1081 acgtttggta aactcgcccg gttcggcaag gcgcgcgccc aactccaaac agcggttcag
     1141 cagcatatcc atcaccaccg gcccgccgtg tccctgaagc tcgatgacat cttcacccgt
     1201 aaaacttgcc ggtgcggcaa aaaacagcaa aagcccgctg tcgattgcct gaccgtccgc
     1261 gtccgtaaaa tcagcatagg ttgccacgcg cggcttgggc gttttcccac acaaagcctc
     1321 tgccatcggc agcaggtttt tccccgatat gcgtatcacg cccacgccgc cgcgccctga
     1381 tgcggtagcg actgccgcaa tcgttggaac gttatccgac ataaaacccc cgaaaattca
     1441 aaacagccgc gattatagca aatgccgtct gaagtccgac ggtttggctt tcagacggca
     1501 taaaaccgca aaaacgcttg ataaatccgt ccgcctgacc taatataacc atatggaaaa
     1561 acgaaacaca tacgccctcc tgctcggtat aggctcgctg ctgggcctgt tccatcccgc
     1621 aaaaaccgcc atccgcccca atcccgccga cgatctcaaa aacatcggcg gcgattttca
     1681 acgcgccata gagaaagcgc gtaaatgacc gaaaacgcac aggacaaggc gcggcaggct
     1741 gtcgaaaccg tcgtcaaatc cccggagctt gtcgagcaaa tcctgtccga cgagtacgtg
     1801 caaataatga tagcccggcg tttccattcg ggaccgttgc cgccgccgtc cgacttggcg
     1861 caatacaacg acattatcag caacggggca gaccgcatta tggcaatggc ggaaaaagaa
     1921 caagccgtcc ggcacgaaac catacggcaa gaccaaacct tcaacaggcg cgggcaactg
     1981 tacggcttca tcagcgtcat cctgatactg ctttttgccg tcttcctcgt atggagcggc
     2041 taccccgcaa ccgccgcctc ccttgccggc ggcacagtgg ttgccttggc gggtgctttc
     2101 gtgattggaa gaagccgaga ccaaggcaaa aattaatgac aaatcctagg gcgtgcttca
     2161 tatccgcccg aacgccgaac cgcacatata ggcacatccc gcgcgccgcc cggaagcgga
     2221 agccgcgccc tcccaaacaa acccgaatcc cgtcagataa ggaaaaataa tgaaaacaac
     2281 cgacaaacgg acaaccgaaa cacaccgcaa agccccgaaa accggtcgca tccgcttctc
     2341 gcctgcttac ttagccatat gcctgtcgtt cggcattctt ccccaagcct gggcgggaca
     2401 cacttatttc ggcatcaact accaatacta tcgcgacttt gccgaaaata aaggcaagtt
     2461 tgcagtcggg gcgaaagata ttgaggttta caacaaaaaa ggggagttgg tcggcaaatc
     2521 aatgacaaaa gccccgatga ttgatttttc tgtggtgtcg cgtaacggcg tggcggcatt
     2581 ggtgggcgat caatatattg tgagcgtggc acataacggc ggctataaca acgttgattt
     2641 tggtgcggag ggaagcaatc ccgatcagca ccgtttttct tatcaaattg tgaaaagaaa
     2701 taattataaa gcagggacta acggccatcc ttatggcggc gattatcata tgccgcgttt
     2761 acataaattt gtaaccgatg cagaacctgt tgaaatgacc agttatatgg atgggcggaa
     2821 atatatcgat caaaataatt accctgaccg tgttcgtatt ggggcaggca ggcaatattg
     2881 gcgatctgat gaagatgagc ccaataaccg cgaaagttca tatcatattg caagtgcgta
     2941 ttcttggctc gttggtggca atacctttgc acaaaatgga tcaggtggtg gcacagtcaa
     3001 cttaggtagt gaaaaaatta aacatagccc atatggtttt ttaccaacag gaggctcatt
     3061 tggcgacagt ggctcaccaa tgtttatcta tgatgcccaa aagcaaaagt ggttaattaa
     3121 tggggtattg caaacgggca acccctatat aggaaaaagc aatggcttcc agctggttcg
     3181 taaagattgg ttctatgatg aaatctttgc tggagatacc cattcagtat tctacgaacc
     3241 acgtcaaaat gggaaatact cttttcacga caataataat ggcacaggaa aaatcgacgc
     3301 caaacatgaa cacaattctc tgcctaatag attaaaaaca cgaaccgttc aattgtttaa
     3361 tgtttcttta tccgagacag cacaagaacc tgtttatcat gctgcaggtg gtgtcaacag
     3421 ttatcgaccc agactgaata atggagaaaa tatttccttt attgacgaag gaaaaggcga
     3481 attgatactt accagtaaca tcaaccaagg tgctggagga ttatatttcc aaggagattt
     3541 tacggtctcg cctgaaaaca acgaaacgtg gcaaggcgcg ggcgttcata tcagtgaaga
     3601 cagtaccgtt acttggaaag tcaatggcgt ggcaaacgac cgcctgtcca aaatcggcaa
     3661 aggcacgctg cacgttcaag ccaaagggga aaaccaaggc tcgatcagcg tgggcgacgg
     3721 taaagttatt ttagatcaac aagcagatga aaataataaa aaacaagcct ttagtgaaat
     3781 cggcttggtc agcggcaggg gtacggtgca actgaatgcc gataatcagt tcaaccccga
     3841 caaactctat ttcggctttc gcggcggacg tttggatttg aacgggcatt cactttcgtt
     3901 ccaccgtatt caaaataccg atgaaggggc gatgattgtc aaccacaatc aagacaaaga
     3961 atccaccgtt accattacag gcaataaaga tattgctaca accggcaata acaacagctt
     4021 ggatagcaaa aaagaaattg cctacaacgg ttggtttggc gagaaagata cgaccaaaac
     4081 gaacgggcgg ctcaaccttg tttaccagcc cgccgcagaa gaccgcaccc tgctgctttc
     4141 cggcggaaca aatttaaacg gcaacatcac gcaaacaaac ggcaaactgt ttttcagcgg
     4201 cagaccaaca ccgcacgcct acaatcattt aaacgaccat tggtcgcaaa aagagggcat
     4261 tcctcgcggg gaaatcgtgt gggacaacga ctggatcaac cgcacattta aagcggaaaa
     4321 cttccaaatt aaaggcggac aggcggtggt ttcccgcaat gttgccaaag tgaaaggcga
     4381 ttggcatttg agcaatcacg cccaagcagt ttttggtgtc gcaccgcatc aaagccacac
     4441 aatctgtaca cgttcggact ggacgggtct gacaaattgt gtcgaaaaaa ccattaccga
     4501 cgataaagtg attgcttcat tgaccaatac cgacatcagc ggcaatgtca gccttgccga
     4561 tcacgctcat ttaaatctca cagggcttgc cacactcaac ggcaatctta gtgcaaatgg
     4621 cgatacacgt tatacagtca gccacaacgc cacccaaaac ggcgacctta gcctcgtggg
     4681 caatgcccaa gcaacattta atcaagccac attaaacggc aacacatcgg cttcgggcaa
     4741 tgcttcattt aatctaagcg accacgccgt acaaaacggc agtctgacgc tttccggcaa
     4801 cgctaaggca aacgtaagcc attccgcact caacggtaat gtctccctag ccgataaggc
     4861 agtattccat tttgaaagca gccgctttac cggacaaatc agcggcagca aggatacggc
     4921 attacactta aaagacagcg aatggacgct gccgtcaggc acggaattag gcaatttaaa
     4981 ccttgacaac gccaccatta cactcaattc cgcctatcgc cacgatgcgg caggggcgca
     5041 aaccggcagt gcgacagatg cgccgcgccg ccgttcgcgc cgttccctat tatccgttac
     5101 acctccggct tcggcagaat cccatttcaa cacgctgacg gtaaacggca aattgaacgg
     5161 tcagggaaca ttccgcttta tgtcggaact cttcggctac cgcagcgaca aattgaagct
     5221 ggcggaaagt tccgaaggca cttacacctt ggctgtcaac aataccggca acgaacctgc
     5281 aagcctcgaa caattgacgg tagtggaagg aaaagacaac aaaccgctgt ccgaaaacct
     5341 taattttacc ctgcaaaacg aacacgtcga tgccggcgcg tggcgttacc aactcatccg
     5401 caaagacggc gagttccgcc tgcataatcc ggtcaaagaa caagagcttt ccgacaaact
     5461 cggcaaggca gaagccaaaa aacaggcgga aaaagacaac gcgcaaagcc ttgacgcgct
     5521 gattgcggcc gggcgcgatg ccgtcgaaaa gacagaaagc gttgccgaac cggcccggca
     5581 ggcaggcggg gaaaatgtcg gcattatgca ggcggaggaa gagaaaaaac gggtgcaggc
     5641 ggataaagac accgccttgg cgaaacagcg cgaagcggaa acccggccgg ctaccaccgc
     5701 tttcccccgc gcccgccgcg cccgccggga tttgccgcaa ccgcagcccc aaccgcagcc
     5761 ccaaccgcag cgcgacctga tcagccgtta taccaatagc ggtttgagtg aattttccgc
     5821 cacgctcaac agcgttttcg ccgtacagga cgaattagac cgcgtatttg ccgaagaccg
     5881 ccgcaacgcc gtttggacaa gcggcatccg ggacaccaaa cactaccgtt cgcaagattt
     5941 ccgcgcctac cgccaacaaa ccgacctgcg ccaaatcggt atgcagaaaa acctcggcag
     6001 cgggcgcgtc ggcatcctgt tttcgcacaa ccggaccgaa aacaccttcg acgacggcat
     6061 cggcaactcg gcacggcttg cccacggcgc cgttttcggg caatacggca tcgacaggtt
     6121 cgacatcggc atcagcgcgg gcgcgggttt tagcagcggc aacctttcag acggcatcgg
     6181 aggcaaaatc cgccgccgcg tgctgcatta cggcattcag gcacgatacc gcgccggttt
     6241 cggcggattc ggcatcgaac cgcacatcgg cgcaacgcgc tatttcgtcc aaaaagcgga
     6301 ttaccgctac gaaaacgtca atatcgccac ccccggcctt gcgttcaacc gctaccgcgc
     6361 gggcattaag gcagattatt cattcaaacc ggcgcaacac atttccatca cgccttattt
     6421 gagcctgtcc tataccgatg ccgcttcggg caaagtccga acgcgcgtca ataccgccgt
     6481 attggctcag gatttcggca aaacccgcag tgcggaatgg ggcgtaaacg ccgaaatcaa
     6541 aggtttcacg ctgtccctcc acgctgccgc cgccaaaggc ccgcaactgg aagcgcaaca
     6601 cagcgcgggc atcaaattag gctaccgctg gtaacaccgg atatgccgaa aggggtttga
     6661 cgatgccgcc gtgcgctgtc aaaccctttt tctgccgccg ccttgtgcct gctccattgt
     6721 ctgataaccg tcaaattgat ttttagccca tgtttggtgg gtcggggaaa tctatatctt
     6781 cgtccgtgcc gaaatagtct atgcccgata tacaattttg atacacaaac ttggaaatat
     6841 cggtatcgtc gccggagcga tagaatgcgg acagtttttc attgaattgc ggcatctgca
     6901 tttcggagat ttccaagatg ccgcagcccc ccgccatcat cagaccgttg gcaaataagg
     6961 ttgccgttcg tttgttgccg tcccaaaaaa cctgctgccg catacaataa agcataaaac
     7021 ggacggctgc ctccgtcgtc gaaccgcttt gcagtccgat attttgtaac acgcgagcca
     7081 cttcaatttc cttcactgga ttcggggcat gacgggaacc gtccaatagc gttacgccga
     7141 ccgaaccggt acggaaatca c
//
LOCUS       DAWFHY010000096         6758 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.096, whole genome shotgun sequence.
ACCESSION   DAWFHY010000096 DAWFHY010000000
VERSION     DAWFHY010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6758
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.096"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(307..1599)
                     /locus_tag="WGQ68_001807"
     CDS             complement(307..1599)
                     /locus_tag="WGQ68_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEZ0715572.1"
                     /translation="MAVFPLSAKHRKYALRALAVSIILVSAAYIASTERTERVRPQRV
                     EQKLPPLSWGGSGVQTAYWVQEAVQPGDSLADVLARSGMARDEIARITEKYGGEADLR
                     HLRADQSVHVLVGGDGGAREVQFFTDEDGERNLVALEKKGGIWRRSASEADMKVLPTL
                     RSVVVKTSARGSLARAEVPVEIRESLSGIFAGRFSLDGLKEGDAVRLMYDSLYFHGQQ
                     VAAGDILAAEVVKGGTRHQAFYYRSDKEGGGGGNYYDEDGRVLQEKGGFNIEPLVYTR
                     ISSPFGYRMHPILHTWRLHTGIDYAAPQGTPVRASADGVITFKGRKGGYGNAVMIRHA
                     NGVETLYAHLSAFSQAQGNVRGGEVIGFVGSTGRSTGPHLHYEARINGQPVNPVSVAL
                     PTPELTQADKAAFAAQKQKADALLARLRGIPVTVSQSD"
     gene            1677..2645
                     /locus_tag="WGQ68_001808"
     CDS             1677..2645
                     /locus_tag="WGQ68_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715573.1"
                     /translation="MKLTLAIPSLNLDEDETRIPLCLPAFNKILQYGSLHRQSCTASA
                     FYARYLWCGRLAERAAQSLNMPSEAVALATPVWQKMGMHQANVLTAEYLNVGTDEAER
                     LCRDLSAFYGDIPWRFVPVLPELWLVSLPRAYRWGAKPVLDLGGLLGTDDQPDGEDAL
                     EWLRVQTEIQMWLAAHPVNHNRKKRGLPELNGLWLWDSLHGSSQGGTLFADTVWSRFH
                     PDRRALPDNFRAYEETAAHLPDTHHILFMDDLRLTALTGDRERYASVLQQWEEHWFAP
                     IYEAVRTGKIKRLDITTDGQHGGTLTFKPADRWKFWRGAKTFDGIW"
     gene            complement(2846..4312)
                     /locus_tag="WGQ68_001809"
     CDS             complement(2846..4312)
                     /locus_tag="WGQ68_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0715574.1"
                     /translation="MVIFYFCGKTFMPARNRWMLLLPLLASAAYAEETPREPDLRSRP
                     EFRLHEAEVKPIDREKVPGQVREKGKVLQIDGETLLKNPELLSRAMYSAVVSNNIAGI
                     RVILPIYLQQAQQDKMLALYAQGILAQADGRVKEAISHYRELIAAQPDAPAVRMRLAA
                     ALFENRQNEAAADQFDRLKAENLPPQLMEQVELYRKALRERDAWKVNGGFSVTREHNI
                     NQAPKRQQYGKWTFPKQVDGTAVNYRLGAEKKWSLKNGWYTTAGGDVSGRVYPGNKKF
                     NDMTAGVSGGIGFADRRKDAGLAVFHERRTYGNDAYSYTNGARLYFNRWQTPKWQTLS
                     SAEWGRLKNTRRARSDNTHLQISNSLVFYRNARQYWMGGLDFYRERNPADRGDNFNRY
                     GLRFAWGQEWGGSGLSSLLRLGAAKRHYEKPGFFSGFKGERRRDKELNTSLSLWHRAL
                     HFKGITPRLTLSHRETRSNDVFNEYEKNRAFVEFNKTF"
     gene            complement(4554..6668)
                     /locus_tag="WGQ68_001810"
     CDS             complement(4554..6668)
                     /locus_tag="WGQ68_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HEZ0715575.1"
                     /translation="MSHDTESKQASKQASKQASKQASKQASGRVNLLTLSVLSLFCTP
                     YAWGADEYDPEVLPRFILEAAENRDSHVWRGPCSISFDCFGMFRAQLGSNTRSTKIGD
                     DADFSFSDKPKPGTSHYFSSGKTDQNSSEYGYDEINIQGKNYNSGILAVDNMPVVKKY
                     ITEKYGADLKQAVKSQLQDLYKTRPEAWAENKKRTEEAYIAQFGTKFSTLKQTMPDLI
                     NKLVEDSVLTPHSNTSQTSLNNIFNKTLHVKIENKSHVAGQVLELTKMTLKDSLWEPR
                     RHSDIHMLETSDNARIRLNTKDEKLTVHKAYQGGADFLFGYDVRESDKPALTFEDKVS
                     GKSGVVLERRPENLKTLDGRKLIAAEKADSNSFAFKQNYRQGLYELLLKQCEGGFCLG
                     VQRLAIPEAEAVLYAQQAYAANTLFGLRAADRGDDVYAADPSRQKLWLRFIGGRSHQN
                     IRGGAAADGRRKGVQIGGEVFVRQNEGSRLAIGVMGGRAGQHASVNGKGGAAGSYLHG
                     YGGGVYAAWHQLRDKQTGAYLDGWLQYQRFKHRINDENRAERYKTKGWTASVEGGYNA
                     LVAEGVVGKGNNVRFYLQPQAQFTYLGVNGGFTDSEGTAVGLLGSGQWQSRAGIRAKT
                     RFALRNGVNLQPFAAFNVLHRSKSFGVEMDGEKQTLAGRTALEGRFGIEAGWKGHMSA
                     RIGYGKRTDGDKEAALSLKWLF"
BASE COUNT         1422 a         2030 c         1715 g         1591 t
ORIGIN      
        1 tgcaaacact gccggaacgg ataaatccgg attcccaccc acttcgtcat tcccgcgaaa
       61 gcgggaatcc ggtgcgttgg gtttccgtca tttccgataa actcctgccg cgttgtattt
      121 ctagattccc gctttcgcgg gaatgacggc ggatgggttt ccgtgccgtg gtgtccggat
      181 tcccgccggt acaggaatga cggcagtgct tccttggctt aattttattt taatcaactg
      241 caaatgccgt ctgaaaaccg gtttgcagat tttcagacgg cattgttttc gtcgccggtt
      301 caaacttcaa tccgattgcg acacggtaac cggtatgccg cgcaagcgcg caagcagcgc
      361 gtccgccttc tgtttctgcg cggcaaacgc cgccttgtcc gcctgcgtca attccggtgt
      421 cggcaatgcg accgaaacag gattgacggg ctgcccgttg atgcgcgcct cgtaatgcag
      481 gtgcggcccg gtcgaacgcc cggtcgaacc gacaaaaccg atgacctcgc cgccgcgcac
      541 attgccttgt gcctgcgaaa acgcgctcaa gtgcgcgtac agcgtttcca caccgttggc
      601 gtggcgtatc atcaccgcgt tgccgtatcc gcccttccgg cctttaaagg taatcacgcc
      661 gtcggcggaa gccctgaccg gcgttccctg cggtgcggca taatcgatgc ccgtgtgcag
      721 ccgccaagtg tgcaggatgg ggtgcatacg gtagccgaac ggcgaagaaa tgcgcgtata
      781 gaccagcggc tcgatgttga agccgccttt ttcctgcaac accctgccgt cttcatcgta
      841 ataattgccg ccccctcctc cttccttgtc cgaacggtaa tagaacgcct gatgccttgt
      901 gccgccctta acgacttcag ccgccaaaat atcgcccgcc gccacctgct gcccgtggaa
      961 atacaggctg tcgtacatca ggcgcacggc atcgccttcc ttcaaaccgt caaggctgaa
     1021 gcggccggcg aaaatcccgc ttaaggattc gcggatttcg acgggcactt ccgcccgcgc
     1081 cagcgaaccg cgcgccgacg ttttgaccac gaccgaacgc agcgtcggca aaaccttcat
     1141 atccgcctca gaagccgacc gccgccatat gccgcctttc ttttccaaag cgaccagatt
     1201 gcgctcgccg tcttcgtcgg taaaaaactg cacttcgcgc gcgccgccgt cgccgccgac
     1261 caaaacatga accgactggt cggcacgcaa atgccgcaaa tcggcttcgc cgccatattt
     1321 ttccgttatt cgggcaattt cgtcccgcgc catacccgaa cgcgccagca cgtccgccag
     1381 cgagtcgcct ggctgcaccg cctcctgcac ccaatatgcc gtctgaacac cgctgccgcc
     1441 ccaagacagc ggcggcagtt tttgttccac gcgctgcggt ctgacgcgct ccgtcctctc
     1501 tgtcgaagca atgtatgccg ccgacaccaa aataatcgaa acggcaagcg cgcgcagcgc
     1561 gtatttccga tgttttgccg aaagtgggaa gacagccata ttgttccttt ttgacggcgt
     1621 ttgcgccgaa tcctctataa tgttgcgcct gacctgcatt tctgaaacgt caaacgatga
     1681 aactcaccct cgccattccc tcgctcaatt tggatgaaga cgaaacccga atacctctct
     1741 gcctgcccgc cttcaacaaa atcctgcaat acggctcgct gcaccgccaa agctgcacgg
     1801 cttcggcatt ttacgcgcgt tatttgtggt gcggcagact ggcggaacgc gcggcgcaaa
     1861 gcctgaatat gccgtctgaa gccgtcgcgc tcgccacgcc cgtctggcaa aagatgggga
     1921 tgcatcaggc aaatgtgctg acggcggaat atctgaacgt cggtacggat gaagcggaac
     1981 ggctctgccg cgacctgtcg gcgttttacg gcgatattcc gtggcgtttc gttcccgtcc
     2041 tgcctgaatt atggctggtt tcccttcccc gcgcgtaccg ctggggcgcg aagcccgttt
     2101 tggacttggg cggattgttg ggcacggacg atcagccgga cggagaagat gcgttggaat
     2161 ggctgcgcgt ccaaaccgaa atccaaatgt ggctggcggc acaccccgtc aaccataacc
     2221 gcaaaaaacg cgggctgccc gagttgaacg gcttatggct ttgggacagt ctgcacggaa
     2281 gcagtcaagg cggcacgctt ttcgccgata cggtttggag ccgtttccac cccgaccgcc
     2341 gcgcattgcc cgacaacttc cgcgcctatg aagaaacggc ggcacacctg cccgacacgc
     2401 accatatcct gtttatggac gatttgcgcc tgaccgccct gacaggggac agggagcggt
     2461 atgcatcggt tttacagcag tgggaagaac actggttcgc accgatctac gaagccgtcc
     2521 gtaccggcaa aatcaaacgg ctggacatca ccacggacgg acaacacggc ggtacgctga
     2581 ccttcaaacc cgccgacagg tggaaatttt ggcgcggcgc gaaaaccttt gacggaatct
     2641 ggtgaacccg cctgccgcaa ccgcgacaat gccgtctgaa gcccgaatcg ggtttcagac
     2701 ggcattttac ctcgcatcgc cgttatgccg gaaaaaccgg aatgcgtttc cgcacacgcg
     2761 gcggcacgca aagcaccgcc cgaaagccgc cgatgcccgc cgtgataccg ccgccggcag
     2821 ggtttcctcc gaaaaggaac agcaatcaga acgttttatt aaactcgaca aacgcccgat
     2881 ttttctcgta ttcgttgaac acatcgttac tccgcgtttc gcggtgcgac aacgtcaggc
     2941 gcggcgtgat gcctttgaaa tgcaatgccc ggtgccaaag gctcaaggat gtgttcaatt
     3001 ctttatccct gcgcctttcc cctttaaaac cgctgaaaaa gccgggtttt tcataatgcc
     3061 gtttcgccgc gccgaggcgc aacagcgaag acaggccgct gccgccccat tcctgccccc
     3121 aggcaaagcg caggccgtaa cggttgaaat tgtcgccccg gtcggcgggg ttgcgctcgc
     3181 ggtaaaaatc caaaccgccc atccaatatt ggcgcgcatt ccggtaaaac accagcgaat
     3241 tggaaatttg caaatgggta ttgtcggaac gcgcccggcg cgtattcttc aaacgccccc
     3301 actccgccga agacaacgtt tgccatttcg gggtttgcca acggttgaaa taaaggcgtg
     3361 cgccgttggt gtaagaataa gcgtcgttgc cgtaggtgcg gcgttcgtgg aacactgcca
     3421 gcccggcatc tttgcgccgg tcggcaaaac cgatgccgcc ggaaacgcct gccgtcatat
     3481 cgttgaattt cttattcccc ggataaaccc tgccggacac gtcgccgccc gccgtcgtgt
     3541 accagccgtt tttcagcgac cattttttct ccgcgccgag ccggtaattg accgccgtgc
     3601 cgtccacctg tttcgggaaa gtccatttgc cgtactgctg ccgtttcggg gcttggttga
     3661 tattgtgttc gcgggtgacg ctgaagccgc catttacctt ccacgcatcg cgttcgcgca
     3721 atgccttgcg gtacagctcg acctgctcca tcagctgcgg cggcaggttt tccgccttca
     3781 ggcggtcgaa ctggtctgcc gccgcctcgt tctgcctgtt ttcaaacaat gctgccgcca
     3841 aacgcatacg gacggcgggc gcgtcgggtt gggcggcaat caattcccgg taatgggaaa
     3901 tcgcctcctt caccctaccg tctgcctgcg ccaaaatccc ttgtgcataa agtgccaaca
     3961 tcttatcctg ctgcgcctgt tgtaggtaaa tcggcaaaat aacgcggata ccggcaatat
     4021 tgtttgagac cactgcggaa tacatcgcgc gggacaacaa ttcgggattt ttcagcaggg
     4081 tttctccgtc aatctgcaaa acttttcctt tttcccgcac ctgccccggc accttctccc
     4141 tgtcgattgg tttgacctcc gcttcatgaa gcctgaactc gggacggctt ctcaaatccg
     4201 gttcgcgcgg tgtttcttcg gcatatgccg cgcttgccaa taaaggcagc agcagcatcc
     4261 atctgtttcg tgcaggcata aatgtcttcc cacaaaaata aaaaataacc attatcaatc
     4321 ggcggattgt atcagttttt tatgccgctg ttccaaaccc tgccgcccga tgccgcgaca
     4381 atgccgtctg aagccagaat cggggttcaa acggcggcaa aaaaccggcg gttttccgcc
     4441 ggtttcatca ttatgccccg cccttcgggg cagcagggtt cggattggtt tgcggggcgg
     4501 cgcgcggtgc cgtgccggtg tgccgcctgt acgtcaaaac atttcccgac gcatcaaaac
     4561 agccatttga gcgacaatgc ggcttctttg tcgccgtccg tccttttgcc gtatccgatg
     4621 cgtgcggaca tatggccttt ccaaccggct tcaatgccga accgcccttc gagtgccgtc
     4681 ctgcctgcca gcgtctgttt ttcgccgtcc atttccacgc cgaaagattt tgacctgtgc
     4741 aaaacattaa aagcggcaaa aggctgaaga ttgacaccat tacgcaaagc aaaacgggtt
     4801 tttgcccgaa tgccggcgcg gctttgccac tgaccgctgc cgagcagtcc gaccgccgtc
     4861 ccctcgctgt cggtaaagcc gccgtttacg cccaagtagg taaactgagc ctgcggttgc
     4921 aggtaaaacc gcacattatt gccttttccg acaacgcctt ccgccacaag cgcgttgtag
     4981 ccgccttcga cagaagccgt ccaacctttg gttttgtagc gttccgcacg gttttcatca
     5041 ttgatgcgat gtttgaaacg ttggtattgc aaccagccgt ccaaatacgc acccgtttgt
     5101 ttatcgcgca actgatgcca cgcagcataa acacccccgc cataaccatg caaataactg
     5161 cctgccgcac cgcctttgcc gttgactgat gcgtgctggc cggccctgcc gcccatcacg
     5221 ccgattgcca gtcggctgcc ttcattttgc cgtacaaaca cctcgccgcc gatttgcacg
     5281 cctttgcgcc gcccgtccgc agccgcgccg ccccgtatat tttgatgcga ccgaccgccg
     5341 atgaagcgca gccacaattt ttgacgggac ggatcggcgg catacacgtc gtcgcccctg
     5401 tcggcggcac gcagcccgaa caaagtattt gccgcataag cctgttgggc atataaaacc
     5461 gcttccgcct cggggatagc caaacgctgc acgcccaagc aaaatccgcc ttcgcattgc
     5521 ttgagcaata attcgtacag tccctgccgg taattttgtt taaacgcaaa cgaattagag
     5581 tctgcctttt ccgccgcaat cagtttgcgc ccgtcgagcg ttttcagatt ttccggccgg
     5641 cgttccaaaa ccacgccgga ttttccgctg actttgtctt caaaggtcag ggcgggtttg
     5701 tccgactccc gcacgtcgta gccgaacagg aaatccgcgc cgccctgata cgctttatgg
     5761 acggtcagtt tttcatcttt cgtgttcagg cggatccggg cattatcgga agtttccagc
     5821 atatggatgt cggaatggcg gcgcggttcc caaagggaat ctttcagcgt catcttggtc
     5881 agttccaaca cctgtccggc gacgtgggat ttgttttcga ttttgacgtg cagtgtttta
     5941 ttgaagatgt tgttgagact agtctgtgat gtattactat gaggagtgag tacggaatct
     6001 tctaccaatt tattaattaa atcgggcatc gtctgtttga gcgtactaaa ttttgttcca
     6061 aactgtgcta tatacgcctc ctcagtccgt tttttatttt ctgcccaagc ttccggtctt
     6121 gttttgtata aatcctgtaa ttgactttta accgcctgct ttaaatcagc cccatacttc
     6181 tctgtaatat attttttgac aacgggcata ttatcgacgg cgaggatgcc gctattgtaa
     6241 tttttacctt ggatattgat ttcgtcatac ccatattcgg atgaattttg atcggtttta
     6301 ccgctggaaa aataatggga agtgccgggt ttcggcttgt ctgaaaatga aaaatcggca
     6361 tcgtcgccga ttttggtaga acgagtattt gaaccaagct gagctctgaa cataccgaag
     6421 caatcaaaag atatgctgca cggaccgcgc caaacgtggg aatctctgtt ttccgccgct
     6481 tctaaaatga atcgcggaag aacttcagga tcatattcat ccgcacccca agcatatggc
     6541 gtgcaaaaca gcgataaaac agataatgtt aatagattaa cccgaccgct tgcttgcttg
     6601 cttgcttgct tgcttgcttg cttgcttgct tgcttgcttg cttgcttgct ttcagtgtca
     6661 tgagaaattt tcctttgtca agtgttaaaa ttataatgat tatatactac atcgcactac
     6721 atcgcaatga aaaatcagga aaaactaaaa acccctcc
//
LOCUS       DAWFHY010000097         6589 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.097, whole genome shotgun sequence.
ACCESSION   DAWFHY010000097 DAWFHY010000000
VERSION     DAWFHY010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6589
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.097"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            661..2109
                     /gene="gnd"
                     /locus_tag="WGQ68_001811"
     CDS             661..2109
                     /gene="gnd"
                     /locus_tag="WGQ68_001811"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019269816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating NADP(+)-dependent
                     phosphogluconate dehydrogenase"
                     /protein_id="HEZ0715576.1"
                     /translation="MKGDIGVIGLAVMGQNLILNMNDCGFKVVAYNRTIGKVDNFLNG
                     AAKGTDIVGAYSLQDLVDKLAKPRKIMMMVRAGSVVDDFIEQLLPLLEEGDIIIDGGN
                     ANYPDTTRRTHYLAEKGILFVGAGVSGGEEGARHGPSIMPGGDKRAWEAVKPIFQAIA
                     AKTPQGEPCCDWVGKDGAGHFVKMVHNGIEYGDMQLICEAYQFMKDGLGLSCDEMHRV
                     FAEWNKTELDSYLIEITAAILGYKDEGGEPLVEKILDTAGQKGTGKWTGINALDLGIP
                     LTLISEAVFARCVSSFKEQRVQTGKLFARAVTPVEGGKQEWVEALRQALLASKIISYA
                     QGFMLIREAGESYGWDLDYGNTALLWREGCIIRSAFLGNIRDAYEANPDLVFLGADPY
                     FKNILENCLPTWRKVVAKAVECGIPMPCMASAITFLDGYTTERLPANLLQAQRDYFGA
                     HTYERTDKPRGEFFHTNWTGKGGDTASTTYDI"
     gene            2172..3443
                     /gene="waaA"
                     /locus_tag="WGQ68_001812"
     CDS             2172..3443
                     /gene="waaA"
                     /locus_tag="WGQ68_001812"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HEZ0715577.1"
                     /translation="MFQWLYDALWLLAPIWIRRYLDKRSGSAPAYRAHRDERFGKPYP
                     NPVTGAVWIHAVSVGETRAAQPLIRELRQRFPDAPLLMTQMTPTGRETAQVLFPDAQC
                     RYLPYDKKTWVRQFLREHRPMFGILMETEIWPNLMKECRKSGVPLFLANARLSEKSLN
                     GYLKVRRLIRPAAASLTGCLAQTEADAARLAKLGAASVQVCGNTKYDLMPSEQMKTLA
                     GQFKKRIGGRPVAMCGSTRVYRGEDEAEKLLAAWQQYRGDALLVIVPRHPEHFQTVFE
                     TAKRFGFKVQRRSDGLPVEPDTQVWIGDSMGELYAYYLCADVAFVGGSLVDSGCQNII
                     EPLSCGVPTIFGFSTYNFSEACRHALASGAAVQVESADAWREAVEKNLSAEGGGMQMQ
                     ARVDGFIAQHRGAGARIAEAVWEAVCGHRGR"
     gene            3484..3933
                     /locus_tag="WGQ68_001813"
     CDS             3484..3933
                     /locus_tag="WGQ68_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family copper resistance protein"
                     /protein_id="HEZ0715578.1"
                     /translation="MSIYAVAHIVHLYCAIAFVGGVFFEVLVLSVLHTGRVSCEARRE
                     VEKAMSYRAVRVMPFVVGLLFASGIVMAANRYLSILGEPFATSFGTMLTLKILLAFSV
                     LAHFAIAVVKMARSTLTVGWSKYIHTVVFTHMLLIVFLAKAMFYISW"
     gene            3966..4940
                     /locus_tag="WGQ68_001814"
     CDS             3966..4940
                     /locus_tag="WGQ68_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HEZ0715579.1"
                     /translation="MTEYPGIGSPLFYGVFFAAVLVMIALDMFSLKKNGSHKVGVKEA
                     LAWSGLWVAVSCLFAGWLYFELAGNLGYGAAVAKEKVLEFFTGYILEKSLAVDNIFVF
                     LMIFGYFKVAPQFQHRVLLYGVLGALVLRTVMIFVGAALVQQFEWILYLFGAFLLYTG
                     IHMMKPEGDEKEDLANSRLLNAVKKVVPVGTEFHGEKFFTVENGKKIATPLFLVLVMI
                     ELSDVVFAVDSIPAVFAVTTDPFIVLTSNIFAILGLRAMYFLLADVAERFIFLKYGLA
                     FVLGFIGVKMLVMHWVHIPISVSLSVVFGALGASVLTSLVYTKKQSDK"
     gene            5009..5084
                     /locus_tag="WGQ68_001815"
     tRNA            5009..5084
                     /locus_tag="WGQ68_001815"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5042..5044,aa:Lys,seq:ttt)
     gene            5303..6556
                     /gene="murA"
                     /locus_tag="WGQ68_001816"
     CDS             5303..6556
                     /gene="murA"
                     /locus_tag="WGQ68_001816"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEZ0715580.1"
                     /translation="MDKLKISANGPLNGEITVSGAKNAALPLMCAGLLTSGALRLKNV
                     PMLADVKTTQKLLQGMGARVLTDNISEFEINGGTVNNTCAPYELVRTMRASILVLGPT
                     LARFGEAQVSLPGGCAIGSRPVDQHLKGLEAMGAEIVIEHGYVRAKGKLKGTRVAMDV
                     VTVGGTENLLMAATLAEGTTVLENCAIEPEVVDLAECLVKMGAKISGIGTSTMIVEGV
                     DELHGCEHSVVPDRIEAGTFLCAVAITGGRVVLRNAAPKTMEVVLDKLVEAGAVIEAG
                     DDWIAIDMRQRPKAVDIRTVVHPGFPTDMQAQFMALNAVAEGSCRVVETIFENRFMHV
                     PELNRMGANITTEGNTAFVQGVEQLSGAVVKATDLRASASLVIAGLAARGETVVEQIY
                     HLDRGYENIEKKLGSVGAKIERVSG"
BASE COUNT         1518 a         1594 c         1907 g         1570 t
ORIGIN      
        1 actttcgtgg gaatgacggg atgtaggttc gtggaaatga cgcggtacag gtttccgtta
       61 cgtatcccca tccgaaagtt tatttataca caagcaatcc aaagttatct gactgaaata
      121 ttgtgagatt tacccgcaaa tctcattttt tccaccactt atacacaaat tcggatgcgc
      181 actctgccga tatgcagaaa taagacattc atgccgacac ctgaccattt ccacaagttc
      241 caaacacact ttcctctcaa acctccacag tattccggtt cttaaagaca tcggcagcca
      301 ttcggtaaag aaaagcggaa tgccatctgc acaacaagca aactgtccac aaaaactatt
      361 cttcacgaaa aacaatccac agttatgcac aaaagggaat ccgccttacc gacaccctaa
      421 aaaaaggtaa tcctgatgat ttttaaaata aaaaaaagtt acccacagaa aatttctcct
      481 atcaatataa tcatcgtttt attattttaa ttttatattt tatttataag ccttcaggga
      541 aacgcacggt aaagcgtaaa ggcatctttt caaaaaatta aaaagttaag gattgttaaa
      601 caagtgtaaa tcaggtagag ttgcggtcga taaattaaat ctataacgga gcttcctgca
      661 atgaaaggcg atattggcgt aatcggtttg gcggttatgg ggcaaaacct gattttgaat
      721 atgaacgatt gcggatttaa ggtggttgcc tacaaccgaa caatcggtaa ggtggacaac
      781 tttttaaacg gcgcggctaa gggaacggat attgtcggcg cgtattccct gcaagacttg
      841 gtcgataagc tggcaaaacc gagaaaaatc atgatgatgg ttcgcgcagg ttcggtggtt
      901 gacgacttta tcgaacaact gcttccgttg ttggaagaag gcgacatcat tatcgacggc
      961 ggcaatgcca attaccccga cacgacacgg cggacacatt accttgccga aaaagggatt
     1021 ttgtttgtcg gcgcgggcgt atccggcggc gaagaaggcg cgcggcacgg cccgtccatt
     1081 atgccgggcg gggataagcg cgcttgggaa gcggttaagc cgatttttca ggcgattgcc
     1141 gccaaaacgc cgcagggcga accgtgttgt gactgggtcg gcaaagacgg cgcggggcat
     1201 tttgtcaaaa tggtgcataa cggcatcgaa tacggcgata tgcagctgat ttgcgaagcg
     1261 taccagttta tgaaagacgg tttgggattg tcctgcgacg aaatgcaccg cgtgtttgcc
     1321 gagtggaaca aaaccgagct ggattcttat ctgattgaaa tcacagcggc aattttgggc
     1381 tataaagacg aaggcggcga accgctggtc gaaaaaatcc tcgatacggc agggcagaaa
     1441 ggcacgggca agtggacggg tatcaacgcc ttggatttgg gcattccgct gacgctgatt
     1501 tccgaagcgg tgttcgcccg ctgcgtttcg tcgttcaaag aacagcgcgt gcagaccggc
     1561 aagctattcg cccgagccgt tacgcccgtc gaaggcggca aacaagaatg ggtcgaggcg
     1621 ttgagacagg ccctgcttgc atccaaaatc atttcctacg cacaaggctt tatgctgatc
     1681 cgcgaagcgg gcgaaagcta cggctgggat ttggactacg gcaacactgc gctgctgtgg
     1741 cgcgaaggct gcatcatccg cagcgcgttt ttgggcaata tccgcgacgc gtatgaggcc
     1801 aatcctgatt tggtgttctt gggtgcggat ccgtatttca aaaatatttt ggaaaactgc
     1861 ctgccgacgt ggcgcaaggt ggttgctaag gcggtcgaat gcggcatccc tatgccctgc
     1921 atggcttcgg cgattacgtt cttggacggc tacacgaccg aacgtctgcc tgccaacctc
     1981 ttgcaggcac agcgcgacta cttcggcgcg cacacttacg agcgcaccga caaaccgcgc
     2041 ggcgagtttt tccacaccaa ctggacgggc aagggcggag ataccgcttc gacaacctac
     2101 gatatttgat tccgttgccg aaatgccgtc cgaaccgttc ggacggcatt tgttttgcag
     2161 gaaatagaaa gatgttccaa tggctttatg atgcattgtg gctgcttgcg ccgatatgga
     2221 tacggcgtta tttggacaaa cgctccggaa gtgccccggc atatcgggcg catcgggacg
     2281 agcgtttcgg caagccgtat ccgaatcccg ttaccggcgc ggtttggata cacgccgttt
     2341 cggtcggaga aacgcgtgcc gcccaaccct tgatacgcga gttgcggcag cgttttcccg
     2401 atgcgccgct gctgatgacg cagatgaccc cgacgggacg ggaaaccgcg caagttctgt
     2461 ttcctgatgc gcaatgccgc tatcttccgt atgacaaaaa aacgtgggta cggcagtttt
     2521 tgcgcgaaca ccgcccgatg ttcggcattt tgatggaaac cgaaatctgg cccaatctga
     2581 tgaaagaatg ccggaaatcg ggcgttccgc tgtttttggc gaatgcgcgg ctgtcggaaa
     2641 aatcgctaaa cggttatctg aaggtccgcc gcctgatccg tcctgccgcc gcttcgctga
     2701 cgggttgtct ggcgcagacg gaggcggatg cggcgcggtt ggcgaaattg ggcgcggcat
     2761 ccgtgcaggt gtgcggcaat accaaatacg acctgatgcc gtctgaacag atgaaaacgc
     2821 tggcggggca gtttaaaaaa cgcatcggag gccggccggt tgccatgtgc ggcagcacgc
     2881 gcgtttatcg gggcgaagac gaggcggaaa aactgctggc ggcgtggcaa caatatcgcg
     2941 gcgatgccct gctggtcatc gtgccgcgcc atcccgagca ttttcagacg gtatttgaaa
     3001 cggcaaaacg cttcgggttt aaggttcagc ggcgcagcga cggtttgccg gtcgaacccg
     3061 atacgcaggt gtggataggc gacagtatgg gcgagctgta tgcgtattac ctgtgcgccg
     3121 atgtcgcttt tgtcggcggc agtctggttg attcgggctg tcagaacatc atcgaaccgc
     3181 tttcctgcgg cgttccgacg atattcggct tttcgaccta caatttttcc gaagcctgcc
     3241 gacacgcctt ggcatcgggt gcggcggttc aagtcgaatc ggcggacgcg tggcgggaag
     3301 ccgttgaaaa aaacttatct gctgaggggg gcggaatgca gatgcaggcg cgcgtggacg
     3361 gctttatcgc acaacatcgc ggagcgggcg cgagaatcgc cgaggcggta tgggaagcgg
     3421 tatgcggaca tcgggggcga tagtgataca atccgtatcc gagttttccg gttggagcat
     3481 cgtatgagta tttatgccgt cgcgcacatc gtccacctgt attgcgccat cgcctttgtc
     3541 ggcggcgtgt tttttgaagt gctggttttg tccgtcctgc atacgggacg ggtgtcgtgc
     3601 gaggcgcggc gcgaagtgga aaaggccatg tcttaccgcg ccgtcagggt gatgccgttt
     3661 gtggtcggac tgctgttcgc cagcggcatc gtgatggcgg caaaccgcta tctttctata
     3721 ttgggcgaac cgtttgccac ttccttcggt acgatgctga cgctgaaaat cctgttggcg
     3781 ttcagcgtgt tggcgcactt cgccatcgcc gtcgtcaaaa tggcgcgttc cacactgacc
     3841 gtcggctggt cgaaatacat acacaccgtc gtctttaccc atatgctgct gattgtcttt
     3901 ttggcaaaag cgatgtttta tatcagctgg taacacaatt acatttcaaa tcaggagatt
     3961 gaaccatgac agaatatccc ggcatcgggt cgccgttgtt ttacggcgtt ttttttgcgg
     4021 cagtgctggt catgattgcc ttggatatgt tttcgctgaa gaaaaacggc agccataaag
     4081 tcggcgtcaa agaagccttg gcatggagcg gcttgtgggt tgccgtatcc tgcctgttcg
     4141 cgggctggct gtattttgaa ctcgccggca atctcggcta cggcgcggca gtcgcaaaag
     4201 aaaaagtatt ggaattcttt accggctaca ttttggaaaa atcgttggca gtcgacaata
     4261 ttttcgtgtt cctgatgata ttcggctact tcaaagtcgc accacagttt cagcaccgcg
     4321 tgctgctgta cggcgtattg ggcgcattgg tattgcgcac cgtcatgatt ttcgtcggcg
     4381 cggcactggt tcagcagttt gagtggattc tgtatctgtt tggcgcgttc ctgctctata
     4441 ctggtataca catgatgaag cccgaaggcg atgagaaaga ggatttggca aacagcaggc
     4501 tgctgaatgc cgtcaagaaa gtcgttccgg ttggcacgga atttcacggc gagaaatttt
     4561 ttaccgtcga aaacggcaaa aaaatcgcca cgccgctgtt tttggtgctg gtcatgattg
     4621 aattgagcga tgtcgtgttt gccgtggaca gtattcccgc cgtctttgct gttaccaccg
     4681 atccgtttat cgtgctgact tccaatattt tcgcaattct gggcttgcgg gcgatgtatt
     4741 tcctgctggc ggatgtggcg gaacgcttta tcttcctgaa atacggcttg gcattcgtgt
     4801 tgggttttat cggtgtgaaa atgctggtaa tgcattgggt gcatatcccg atttccgttt
     4861 cgctgtcggt cgtgttcggc gcattgggtg catcggtgtt gacttcattg gtttatacga
     4921 aaaaacagtc cgataaataa aatgccgtct caacgccgac aaaatggttg acagcatcag
     4981 gcttgatgta tagaatttcg agcttgtcgg gtcgttagct cagtcggtag agcagcggac
     5041 ttttaatccg ttggtcgagc gttcgaatcg ctcacgaccc accagataaa acaaaaaagc
     5101 tgagttttca aaactcggct ttttttgttt tccgacaatc cttgttcgga aagcgcggca
     5161 tttcgtcaaa ttctttggtg cggcttccgt atttttcccg aaaatccctt ataattccgc
     5221 ctctgcaacc ttcatcgggt gatgctttat gcgttgtccc gagtattgaa atattacaag
     5281 tacccaccag gaaagaacaa acgtggacaa actgaaaatc tccgcaaacg gcccgctcaa
     5341 cggggaaata acggtctcgg gcgcgaaaaa cgcggcattg ccgctgatgt gcgcgggttt
     5401 gctgacatcg ggtgcgttgc gcctgaaaaa cgtccctatg ctggcagatg tgaaaaccac
     5461 gcaaaagctg cttcagggga tgggcgcgcg cgtcctgact gacaatatca gcgaatttga
     5521 aatcaacggc ggtacggtaa acaatacctg cgccccttac gaattggtac ggacgatgcg
     5581 ggcctcgatt ttggtactgg ggcctacttt ggcacgtttc ggcgaggcgc aagtcagcct
     5641 gccgggcggc tgcgccatcg gttcgcgccc cgtcgatcag catttgaaag gcttggaagc
     5701 gatgggtgcg gaaatcgtca tcgaacacgg ttacgtcagg gctaagggta aactcaaagg
     5761 tacgcgcgtg gcgatggatg tcgttaccgt cggcggcacg gaaaacctgc tgatggcggc
     5821 gacgctggcg gaaggtacga cggttttgga aaactgcgcc attgagcctg aagtggtcga
     5881 tttggcggaa tgcctggtca aaatgggcgc gaaaatcagc ggcatcggta cgtccacaat
     5941 gattgtggaa ggggtggacg agctgcacgg ctgcgaacac agcgtcgtcc ccgaccggat
     6001 tgaagcaggg acgttcctgt gcgcggtggc gataaccggc ggcagggtgg ttttgcggaa
     6061 tgccgcgccg aaaacgatgg aagtggtgtt ggacaaactg gttgaggcag gtgcggtgat
     6121 tgaggcgggc gacgattgga ttgccatcga tatgcggcag cgtccgaagg cggtggacat
     6181 ccgcacggtc gtccaccccg gcttccccac ggatatgcag gcgcagttta tggcattgaa
     6241 tgccgtggca gagggaagct gccgcgtggt ggaaacgatt tttgaaaacc gctttatgca
     6301 cgtccccgag ttgaaccgga tgggggcgaa catcacaacc gagggcaata cggcatttgt
     6361 gcagggtgtg gaacagcttt ccggcgcggt cgttaaggcg acggatttgc gtgcgtccgc
     6421 aagcctcgtt atcgccggtt tggcggcgcg cggcgaaacc gtggtcgaac agatttacca
     6481 cttggatcgc ggttatgaaa atattgaaaa aaaactcggc agcgtcggcg cgaaaatcga
     6541 gcgcgtatcc ggctgatcgg gttgcgatgc cgtctgaagc cttcagacg
//
LOCUS       DAWFHY010000098         6565 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.098, whole genome shotgun sequence.
ACCESSION   DAWFHY010000098 DAWFHY010000000
VERSION     DAWFHY010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.098"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            500..946
                     /gene="yciA"
                     /locus_tag="WGQ68_001817"
     CDS             500..946
                     /gene="yciA"
                     /locus_tag="WGQ68_001817"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002230436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HEZ0715581.1"
                     /translation="MQHEEGNRQRPQGELLLRTVAMPRDTNPNQDIFGGWIMSQMDLG
                     GGILAAEIARGRIVTVAVQEMNFIRPVKVGNVVCCYGHCVRVGNTSLQLKVEVWVKTL
                     MNDCVTEDRHLVTEAVFTYVAIDAEGNPRPIPKESNPKLSGLLPTP"
     gene            complement(997..1818)
                     /locus_tag="WGQ68_001818"
     CDS             complement(997..1818)
                     /locus_tag="WGQ68_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEZ0715582.1"
                     /translation="MAVLITGASAGFGEAMCRAFVGAGYRVIGAARRADRLQALADES
                     GALFYPLEMDVSRRESVENALNGIPDEFSDIDCLINNAGLALGLDTADKADFEDWETM
                     IQTNVLGLAFLTRKILPKMVERGGGYVMNLGSIAGSYAYPGSNVYGATKAFVRQFSLN
                     LRAELADKNIRVTNIEPGLCGNTEFSNVRFKGDNERAAGVYEGVEFIRPEDIAETALW
                     LYCRPAHMNVNTIEIMPVAQTFAGMKVIKTAVPEVREDFEKQSMSLFSRIRSWFK"
     gene            2072..2530
                     /locus_tag="WGQ68_001819"
     CDS             2072..2530
                     /locus_tag="WGQ68_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEZ0715583.1"
                     /translation="MNIQTRLAATALALFLSACGNGGTPAQPKGEISENRTAAFKSMM
                     PDFSRMGKMVKGEEPYDVEKFKQAAAAFAESSKKPFTLFDSDPQGNGRALPAVWSDGA
                     KFEAEKTKFAAAVEKLNAAAQTGKLDEIKAAYGETGASCKSCHDSFRAPE"
     gene            complement(2673..3809)
                     /gene="lst"
                     /locus_tag="WGQ68_001820"
     CDS             complement(2673..3809)
                     /gene="lst"
                     /locus_tag="WGQ68_001820"
                     /EC_number="2.4.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyllactosaminide
                     alpha-2,3-sialyltransferase"
                     /protein_id="HEZ0715584.1"
                     /translation="MGLKKACLTVLCLIVFCFGIFYTFDRVNHGERNAVSLLKDKLFN
                     EEGEPVNLIFCYTILQMKVAERIMAQHPGERFYVVLMSENRNEKYDYYFNQIKDKAER
                     AYFFYLPYGLDKSFNFIPTMAELKVKSMLLPKVKRIYLASLEKFSIAAFLSTYPDAEI
                     KTFDDGTGNLIQSSSYLGDEFSVNGTIKRNFARMMIGDWSIAKTRNASDEHYTIFKGL
                     KNIMDDGRRKMTYLPLFDASELKAGDETGGTVRILLGSPDKEMKEISEKAAKDFNIQY
                     VAPHPRQTYGLSGVTTLNSPYVIEDYILREIKKNPHTRYEIYTFFSGAALTMKDFPNV
                     HVYALKPASLPEDYWLKPVYALFTQSGIPILTFDDKNQSYGKSK"
     gene            3910..4014
                     /locus_tag="WGQ68_001821"
                     /pseudo
     CDS             3910..4014
                     /locus_tag="WGQ68_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            4242..6467
                     /locus_tag="WGQ68_001822"
     CDS             4242..6467
                     /locus_tag="WGQ68_001822"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEZ0715585.1"
                     /translation="MTQKSTIVYTHTDEAPALATQSLLPIVQAFARHADIDVKTSDIS
                     LSGRILAAFPEYLTEAQRVPDALAELGELVKQPDANVIKLPNISASVPQLTAAIKELQ
                     SKGFAVPDYPADPQTDEEKAVRERYDRIKGSAVNPVLREGNSDRRAPKAVKNFAKKNP
                     HSMGAWTKDSKTHVATMQSGDFFHNEQSVTVPEATSVSIVFTDKQGNKKELREPVALK
                     AGEIIDATVMSKKALLAFLAEQVKDAKAKGVLFSLHMKATMMKVSDPIIFGHAVKVFF
                     APVFEKFGDKLAAAGVNVNNGFGNLLANLDKLDADTRAAVEAEIAAVYAANPDLAMVD
                     SDKGITNLHVPSDVIVDASMPAMIRNSGRMWDKDGKAQDTKAVIPDSSYAGVYQATID
                     FCREHGAFDPTTMGTVPNVGLMAQAAEEYGSHNKTFEIEADGQVQVIDAAGKVLMQHD
                     VEAGDIWRMCQTKDAPVKDWVQLAVNRARLSNTPAVFWLDENRPHDKSLLAKVKAYLA
                     ELDTDGLDIRVLAPEEAAKFSLGRLKNGEDTISVTGNVLRDYLTDLFPILELGTSAKM
                     LSIVPLMNGGGMFETGAGGSAPKHVQQFLEENHLRWDSLGEFLALAVSFEHLAQKTGN
                     AKAQVLADTLDAATEKLLLNDKSPKRKAGELDNRGSHFYLTLYWAQELAAQDKDAELK
                     AAFTPLAAALTADEAKIVAELSAVQGKAADIGGYYAANPEKAAQVMRPSVTFNQALNA
                     L"
BASE COUNT         1762 a         1919 c         1465 g         1419 t
ORIGIN      
        1 gttgcgttcc gacaacaccg taatcttgaa atccgtccga caacaccgta atcttaaaac
       61 ccgtcattcc cgcgcaggtg ggaatccaga ccccctgacg cggcgggaat ctatcgaaaa
      121 tgactgaaac cccgagattc tagattccca ctttcgtggg aatgacgtgg tgcaggtttc
      181 cgtatggatg gattcgtcat tcccgcgcag gcgggaatcc agaccccctg acgcggcggg
      241 aatctatcgg aaatgactga acccccgaga ttctagattc ccactttcgt gggaatgacg
      301 gttcagttgc ttagggtagg atttggcggg attggcggac tgaagcccac tctacagccc
      361 cgccctacaa tacgccttgc gaatctgtcc cacgcccgcc cttgtatgtc tcaaatgggt
      421 taaactacgc acttcccgac ttcccgtttc gggcaggggt gcaggttcga tttatctttc
      481 aataaaacaa ggaaacttta tgcagcacga agaaggcaac cgccaacgcc ctcaaggcga
      541 actgctcctg cgtaccgtcg ctatgccgcg cgataccaat cccaaccaag acattttcgg
      601 cggctggatt atgtcgcaga tggatttggg cggcggcata ttggcggcgg aaatcgcgcg
      661 gggacgcatc gttaccgtcg ccgttcagga aatgaacttc atccgcccgg tcaaggtcgg
      721 caacgtcgtc tgctgctacg ggcattgcgt ccgcgtgggc aatacttccc tccagcttaa
      781 agtggaagtc tgggtgaaaa ctttgatgaa cgattgcgtt accgaagacc gccacctcgt
      841 aaccgaagcc gtgttcactt atgtcgccat cgatgcggaa ggcaatccgc gcccgattcc
      901 gaaagaaagc aaccccaaat tgtcgggctt attgcctact ccataaaaat acccgtaaaa
      961 atgccgtctg aaactgtctt cagacggcat tccgtatcat ttgaaccagg acctgatgcg
     1021 ggaaaacagc gacatactct gtttttcaaa gtcttcccgt acttccggca cggctgtttt
     1081 tatgaccttc attcctgcaa aagtctgcgc cacgggcata atttcaatcg tgttcacatt
     1141 catatgcgcc ggccggcagt acagccacaa tgcggtttcc gcaatatctt cggggcggat
     1201 aaattccaca ccctcataca cgcccgccgc cctctcgtta tcgcctttga agcgcacgtt
     1261 ggaaaactcc gtattgccgc acaaacccgg ctcgatattg gtaacgcgga tatttttatc
     1321 tgccaattcg gcacggagat tcaggctgaa ctgacgtaca aacgctttag tcgcaccata
     1381 aacattactg cccggatagg catagctgcc tgcaatcgaa cccaaattca tcacatagcc
     1441 gccgccgcgt tccaccattt ttggcaaaat tttgcgcgtc aggaacgcca aacccaaaac
     1501 attggtttga atcattgttt cccaatcttc aaaatccgcc ttgtccgccg tgtccaaacc
     1561 caaagccagc ccggcattgt tgatgaggca gtcgatgtcg gaaaattcat cggggatgcc
     1621 gttcaaggcg ttttccaccg actcgcggcg cgacacgtcc atttccaaag ggtaaaacaa
     1681 agcacccgat tcatccgcca aagcctgaag cctgtccgca cggcgcgccg caccgatgac
     1741 gcggtatccc gccccgacaa acgcgcggca catcgcttcg ccgaatcctg ccgaagcacc
     1801 ggtaattaaa acagccattg tttttccttt ctttctcgtt tcccaaacct gtttcaggta
     1861 tcataacgcc cttcagacgg catcgcgtcg gctgaaaatg acaacaacca taccaccacg
     1921 acaggacaca ttcaatatgt ttcaacacac aggacgacac ataaagcgtc gccctatgtg
     1981 ccgtcctgat tcggaagggg ttacaccctt cccaaataaa atctgattct accgccctaa
     2041 agggcggggt ttcaaccgaa aaggaaacac gatgaacatt caaacccgcc ttgccgcaac
     2101 cgccctcgcc ctattccttt ccgcctgcgg aaacggcggc acgcccgcac agcccaaagg
     2161 cgaaatttcc gaaaaccgca ccgccgcgtt caaatctatg atgcccgact tctcgcgtat
     2221 gggcaaaatg gtcaaaggcg aagaacctta cgatgtcgaa aaattcaaac aggcggcagc
     2281 ggcgtttgcc gaaagcagca agaaaccgtt tacgcttttt gattccgatc cgcaaggcaa
     2341 cggccgcgcc ctgcccgccg tttggtcgga tggtgcaaaa tttgaagccg aaaaaacaaa
     2401 attcgccgcc gccgtcgaaa aactcaacgc cgcagcacaa accggcaaac tggacgaaat
     2461 caaagccgcc tacggcgaaa ccggtgcaag ctgcaaatcc tgccacgaca gcttccgcgc
     2521 gccggaataa catccgaaac aaaacaatgc cgtctgaagc cttcagacgg cattttcttt
     2581 gccatttcag cacacgcaaa gcaatcaaaa attatcgtca atccaaaaaa tacgctatat
     2641 cctgattttt gccttactta ctccgccatt ctctattttg atttaccata cgattgattt
     2701 ttatcgtcaa atgtcaaaat cgggatgccg gattgggtaa acagggcata cactggcttg
     2761 agccaataat cttctggaag ggaagccggt ttcaatgcgt aaacgtgcac attgggaaaa
     2821 tccttcatcg tcaacgccgc gccgctgaaa aaggtataaa tttcatacct cgtatgcggg
     2881 tttttcttaa tctcgcgcaa aatatagtct tcgatgacat agggcgaatt taatgtggtt
     2941 acgccggaaa gcccgtaggt ttggcggggg tgcggcgcga catattgtat gttgaaatct
     3001 tttgccgcct tttccgaaat ttccttcatt tctttgtcgg gcgaacccaa aagtatccgc
     3061 accgtgccgc ccgtttcatc ccccgccttc aattcggacg catcgaacag cggcaagtaa
     3121 gtcatcttgc ggcggccgtc gtccataatg tttttcaaac ccttgaatat cgtgtaatgc
     3181 tcgtcggaag cattgcgggt tttggcgatg ctccaatctc cgatcatcat ccgggcaaaa
     3241 ttccgcttga tcgtcccgtt tacagaaaac tcatcaccca aatagctgct gctttgaatt
     3301 aaattgcctg tcccgtcgtc aaaggttttg atttctgcat ccgggtaagt gctcaaaaag
     3361 gcggcaatgc tgaatttttc caaacttgcc aaataaatcc gcttgacctt cggcagcagc
     3421 atcgacttta ccttcagctc cgccatcgtc ggaatgaaat taaacgattt gtccaaaccg
     3481 tagggcaagt agaaaaaata cgcccgctcc gccttatcct ttatctgatt gaaataataa
     3541 tcgtattttt cattcctgtt ttcagacatc agcaccacat aaaaccgctc ccccggatgc
     3601 tgcgccataa tcctttccgc caccttcatc tgcaatatgg tatagcagaa aatcagattg
     3661 accggttccc cctcttcatt gaagagtttg tccttcagca gggaaaccgc attcctttcc
     3721 ccatgattta cccggtcaaa tgtataaaat atcccgaaac aaaaaacaat caaacacaac
     3781 acggtcaaac aagccttttt caagcccata tccctaaaac tccattccga caaattgaac
     3841 atacgccccg agtgtcccgg gacggaaaaa tgcggaattt tagcagatat tccgacagtt
     3901 accgaatatt atagtggttt aaatttaaat caggacaagg cgacgaagcc gcagacagta
     3961 caagcagtac gacaaggcaa ggcaacgctg tactggttta aatttaaacc actataaaat
     4021 caatttcacc gaaaatatgc cgccgcacat caggcacagc acctccgacc gccaccaaac
     4081 gcgggaaaac catttccccc ttcctttcac gctgccgcgt tcaaacacgc caagatcgaa
     4141 caaagcgcga acaaagccgc caaaatatgc cataatagcc gcgcacattc cccgcgcaat
     4201 gtgttccaaa agaaaaaacc aacctacaag agaataatcc tatgactcaa aaatccacca
     4261 ttgtttatac ccataccgac gaagcccccg cgctggcgac ccaatcgctg ctgccgattg
     4321 tgcaggcttt tgcccgccac gccgatattg atgtcaaaac cagcgacatt tctctctccg
     4381 gccgtatttt ggcggcgttt cccgaatacc tgaccgaagc gcaacgcgta cctgatgcgc
     4441 ttgccgaatt gggcgaactg gtgaaacaac ccgatgcaaa cgtaatcaaa ctgccgaaca
     4501 tcagcgcgtc cgtaccgcaa ctgactgccg cgattaaaga attgcaatct aaaggctttg
     4561 ccgttcccga ctatcccgcc gacccgcaaa ccgatgaaga aaaagccgta cgcgaacgct
     4621 acgaccgcat caaaggcagc gcggtaaacc ctgtcctgcg tgaaggcaac tccgaccgtc
     4681 gcgcccctaa agcagtgaaa aactttgcga aaaaaaatcc gcacagcatg ggcgcatgga
     4741 ccaaagactc caaaacccac gttgccacca tgcaaagcgg cgactttttt cataacgaac
     4801 aatccgttac cgtacctgaa gcgacttccg tatccatcgt attcaccgac aaacaaggca
     4861 acaaaaaaga gctgcgcgag cccgtcgccc tgaaagccgg cgaaatcatc gacgcgaccg
     4921 tgatgagtaa aaaagccctg ctcgccttcc ttgccgaaca agtaaaagat gcgaaagcaa
     4981 aaggcgtatt gttctcgctg catatgaaag ccacgatgat gaaagtgtcc gacccgatta
     5041 tcttcggaca cgccgtcaaa gtgttctttg cgcctgtttt tgaaaaattc ggcgacaaat
     5101 tggctgccgc cggcgtcaac gtcaacaacg gcttcggcaa cctgcttgcc aatttggaca
     5161 aactggatgc ggacacccgc gcagccgttg aagctgaaat cgccgccgtt tacgccgcca
     5221 accccgattt ggcgatggtt gattccgaca aaggcatcac caacctgcac gttcccagcg
     5281 atgtcatcgt cgatgcctct atgcctgcaa tgattcgcaa ctccggccgt atgtgggata
     5341 aagacggcaa agcgcaagac accaaagccg tgattcccga cagtagctac gcaggcgttt
     5401 atcaagcaac catcgacttc tgccgcgaac acggcgcatt cgacccgaca accatgggta
     5461 ccgttcctaa cgtcggtctg atggcgcaag cagccgaaga atacggttca cacaacaaaa
     5521 ctttcgaaat cgaagccgac ggccaagttc aagtcattga tgcagcgggc aaagtcctga
     5581 tgcagcacga tgttgaagca ggcgacatct ggcgtatgtg tcaaaccaaa gacgcgcccg
     5641 tcaaagactg ggtacaactc gccgtcaacc gcgcccgtct gagcaataca ccagccgtat
     5701 tctggctcga tgaaaaccgt ccgcacgaca agagcctgct cgccaaggtt aaagcctacc
     5761 ttgccgaact ggataccgac ggcctcgaca tccgcgtcct cgctcccgaa gaagccgcta
     5821 aattcagctt aggccgtctg aaaaacggcg aagacaccat ctccgtaacc ggcaacgtcc
     5881 tgcgcgacta cctgaccgac ttgttcccga ttttggaact gggtaccagc gcgaaaatgc
     5941 tgtctatcgt cccattgatg aacggcggcg gtatgtttga aaccggcgcg ggcggttctg
     6001 caccgaaaca cgttcaacaa ttcctcgaag aaaaccactt gcgttgggac tctttgggcg
     6061 aattcctcgc actcgccgta tcgtttgaac atttggcgca aaaaaccggc aacgccaaag
     6121 cccaagtcct cgccgacact ttggatgcag ccaccgaaaa actgctgttg aacgacaaat
     6181 cgcctaaacg caaagcaggc gaactcgaca accgcggcag ccatttctac cttaccctct
     6241 actgggcgca agaattggcg gcgcaagaca aagatgccga actgaaagcc gcatttacgc
     6301 cattggcagc cgctttgacc gccgacgaag cgaaaatcgt tgccgagctt tccgccgtac
     6361 aaggcaaagc ggccgacatc ggcggctact acgccgccaa tcctgaaaaa gccgcacaag
     6421 tgatgcgtcc gagcgtaacc tttaatcagg cactaaacgc cttataagca caaaggtaaa
     6481 aatgccgtct gaacattcgt cgttcagacg gcattagtta tccctatctg cctgattatg
     6541 attaaacgcc gcacccattg gatat
//
LOCUS       DAWFHY010000099         6333 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.099, whole genome shotgun sequence.
ACCESSION   DAWFHY010000099 DAWFHY010000000
VERSION     DAWFHY010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.099"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(213..2141)
                     /gene="dnaK"
                     /locus_tag="WGQ68_001823"
     CDS             complement(213..2141)
                     /gene="dnaK"
                     /locus_tag="WGQ68_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEZ0715586.1"
                     /translation="MAKVIGIDLGTTNSCLAISENGQTKVIENAEGARTTPSVIAYLD
                     GGEILVGAPAKRQAVTNAKNTIYAAKRLIGHKFEDKEVQRDIESMPFEIIKANNGDAW
                     VKAQGKELSPPQISAEVLRKMKEAAEAYLGEKVTEAVITVPAYFNDSQRQATKDAGRI
                     AGLDVKRIINEPTAAALAFGMDKGDNKDRKVAVYDLGGGTFDISIIEIANLDGDKQFE
                     VLATNGDTFLGGEDFDQRLIDYIIDEFKKEQGIDLKQDVMALQRLKEAAEKAKIELSS
                     GQQTEINLPYITMDATGPKHLAMKITRAKFESLVEDLIARSIEPCRTALKDAGLSTGD
                     IDDVILVGGQSRMPKVQEAVKAFFGKEPRKDVNPDEAVAVGAAIQGEVLSGGRSDVLL
                     LDVTPLSLGIETMGGVMTKLIQKNTTIPTKASQVFSTAEDNQSAVTIHVLQGERERAS
                     ANKSLGQFNLGDIAPAPRGMPQIEVTFDIDANGILHVSAKDKGTGKAANITIQGSSGL
                     SEEEIERMVKDAEANAEEDKKLTELVASRNQAEALIHSVKKSLADYGDKLDAAEKEKI
                     EAALKEAEEAVKGDDKAAIDAKAEALGTASQKLGEMVYAQAQAEAQAGESEQANASAK
                     KDDDVVDADFEEVKDDKK"
     gene            complement(2349..2621)
                     /locus_tag="WGQ68_001824"
     CDS             complement(2349..2621)
                     /locus_tag="WGQ68_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715587.1"
                     /translation="MKKTGKYLIYAAAFTAFCFAFQENRSEAKQPDITLSASLCEKFN
                     ILNAKDMDAEQVSLSKECDIIESSHDWEKEYGNLNEQEMLAGIVYE"
     gene            2807..3493
                     /locus_tag="WGQ68_001825"
     CDS             2807..3493
                     /locus_tag="WGQ68_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEZ0715588.1"
                     /translation="METFKDRLVFLWKSEAKQAKIASDIEMTIAGFSRIWNEGGLPKS
                     ETLKKIKQLKGCSIDWLLTGEGNPFPDEAPKKSLAYDTLGNEVDTDEFVFVPRYDIRA
                     AAGYGQFVDHEEPVFTMAFRRHWIENYVTRDTKNLSVISVKGDSMEGVLNDGDSILVN
                     HGENTPRDGLYVLRINENLLVKRLQIVPGGIINVISANEAYPAFEINLNDLTDDVEII
                     GRVEWFGRTI"
     gene            3625..3963
                     /gene="erpA"
                     /locus_tag="WGQ68_001826"
     CDS             3625..3963
                     /gene="erpA"
                     /locus_tag="WGQ68_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HEZ0715589.1"
                     /translation="MSDESPIIFTDSCCAKVADLIAEENNPDLKLRVFVNGGGCSGFQ
                     YGFTFDEIKNDDDFEIEKNGLVFLVDPMSYQYLVGAEIDYTESLQGSQFVIRNPNAET
                     TCGCGSSFSV"
     gene            3980..4126
                     /locus_tag="WGQ68_001827"
     CDS             3980..4126
                     /locus_tag="WGQ68_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715590.1"
                     /translation="MPSERSDGIFTFGKYIIGMNSHIIRLFEDESGFPKGTGGTVSGV
                     FVPL"
     gene            4123..4374
                     /locus_tag="WGQ68_001828"
                     /pseudo
     CDS             4123..4374
                     /locus_tag="WGQ68_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
     gene            4528..4740
                     /locus_tag="WGQ68_001829"
     CDS             4528..4740
                     /locus_tag="WGQ68_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease III domain protein"
                     /protein_id="HEZ0715591.1"
                     /translation="MELETVVPPEEGEGEGEGADGEMPEYLELHGGREDDISAPSQPE
                     PPTRNIKPLLFGLLIAFLGYLLGKIF"
     gene            4775..6286
                     /gene="ubiB"
                     /locus_tag="WGQ68_001830"
     CDS             4775..6286
                     /gene="ubiB"
                     /locus_tag="WGQ68_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HEZ0715592.1"
                     /translation="MKWLKRLTVIVGTFYRYRLAGLCASLMGSGWICALLKMMPQSSK
                     LKNEPPAVRLRLALESLGPIFIKFGQVLSTRPDLIPHDYAVELAKLQDKVPPFDARLS
                     REQIEKSLGQPIEKLYAEFETEPVASASIAQVHKARLHSGEQVAVKVLRPNLLPVIEQ
                     DLSLMRFGAGWVERLFADGKRLKPREVVAEFDKYLHDELDLMREAANASQLGRNFQNS
                     DMLIVPKVFYDYCTSDVLTIEWMDGTPVSDIAKLKADGIDLHKLADYGVEIFFTQVFR
                     DGFFHADMHPGNILVAADNRYIALDFGIVGTLTDYDKRYLAINFLAFFNRDYRRVATA
                     HIESGWVPADTRAEELEAAVRAVCEPVFNKPISQISFGLVLMRLFEVSRRFNVEIQPQ
                     LVLLQKTLLNIEGLGRQLDPDLDLWKTAKPFLVKWINGQVGPKALWRNLKNEAPDWAQ
                     IIPSLPRKISALIDENRQQEMRDAYIHLVKVQQRQSLWLAVIAVVLLLILLLK"
BASE COUNT         1442 a         1409 c         1673 g         1809 t
ORIGIN      
        1 tcgttctctt tgagctaagg cgaggcaacg ctgtactggt ttaaatttaa tccactatat
       61 aaaaaatttc cagagaaccg atacaacagt tggaacttgg gtttgggaat attacggtag
      121 atgaacttgg aacctctgtt atgctatggt cttttatctc aattgaaaaa agcgcgaatc
      181 aaacggttcg cgcttttttc agacggcatc aattattttt tgtcgtcttt tacttcttca
      241 aagtcggcat ctacgacatc atcgtctttc tttgcagaag cattggcttg ttcgctttcg
      301 cctgcttggg cttcagcttg tgcttgagcg taaaccattt cgcccagttt ttggctggct
      361 gtgcccagcg cctcggcttt ggcatcgatg gcggctttgt cgtcgccttt cacggcttct
      421 tcggcttctt tcagcgcggc ttcgattttt tctttttcgg cagcgtcgag tttgtcgccg
      481 tagtccgcca aagatttttt cacggagtga atcagggctt cggcttggtt gcgggaagcg
      541 accaattcag tcagcttttt atcttcctca gcattggctt cggcatcttt caccatgcgt
      601 tcgatttctt cttcgctcaa acctgaagaa ccttggatgg taatgttggc cgctttgcca
      661 gtgcctttgt ctttggcgga aacgtgcaag ataccattgg cgtcgatgtc gaaagtaact
      721 tcaatttgcg gcataccgcg tggtgcaggt gcgatgtcgc ccaagttgaa ctgacccaaa
      781 gatttgttgg cagaagcgcg ttcgcgttcg ccttgcagta cgtggatggt tactgcgctt
      841 tggttgtctt cggcggtaga gaacacttgc gacgctttag tcggaatagt tgtgttcttt
      901 tgaatcagct tggtcatcac gccgcccatg gtttcgatac ccaaggacag aggggttacg
      961 tccagcagca atacgtcgct gcggccgccg cttaacactt cgccttgaat cgctgcgcct
     1021 acggcaacgg cttcgtcggg gttcacgtct ttgcgaggct ctttaccgaa gaaggcttta
     1081 acggcttctt gtactttcgg catacggctt tgaccaccga ccaagattac gtcgtcgatg
     1141 tcgccggtgc tcagaccggc atctttcaat gcggtgcggc aaggctcgat agagcgggca
     1201 atcaggtctt caaccaggct ttcgaatttg gcgcgggtaa ttttcatcgc caagtgtttc
     1261 ggaccggttg cgtccatggt aatgtacggc aggttgattt cggtttgctg gccgctggac
     1321 aattcgattt tggctttttc ggcagcttct ttcagacgtt gcagagccat tacgtcttgt
     1381 ttcaaatcaa tgccttgttc ttttttaaac tcgtcaatga tgtagtcgat caagcgttgg
     1441 tcgaagtctt caccgcccaa gaaagtatcg ccgttggtag ccaatacttc aaattgtttg
     1501 tcgccgtcga ggttggcgat ttcgatgatg gaaatatcga aagtaccgcc gcccaagtca
     1561 tatacggcta ctttgcggtc tttgttgtcg cctttgtcca taccgaatgc caaagcggct
     1621 gcggtcggct cgttgatgat gcgtttcacg tccaaaccgg cgatacggcc tgcgtctttg
     1681 gtagcttgac gttggctgtc gttgaagtag gcagggacgg taatcacggc ttcggttact
     1741 ttttcgccca agtaagcttc ggcggcttct ttcattttac gcaggacttc tgcggaaatt
     1801 tgaggaggag acagctcttt gccttgtgct tttacccatg cgtcgccgtt gttggctttg
     1861 atgatttcga aaggcataga ttcgatgtcg cgttggactt ctttgtcttc aaatttgtgg
     1921 ccgatcaggc gtttggcggc gtaaatggtg tttttagcgt tggttacggc ttggcgtttg
     1981 gcaggcgcac cgacgaggat ttcgccgccg tccaaataag cgataacgga cggcgtggtg
     2041 cgtgcgcctt ctgcgttttc gatcactttg gtttgaccgt tttcggaaat ggccaaacaa
     2101 gagttggttg tacctaagtc gataccgatt acttttgcca tgtggataat cctatttgat
     2161 tttgcttatt ttgagaaata tgttggaaca ttttgtcccg atgggctgta aatagggcgg
     2221 gcggcgggct gtttcaagct acagcatggc tataagtata taactttatg aatatattgg
     2281 ttttatattt gatttaatac atttggctcc aatgcattta agcataatat ttcaaatggc
     2341 aggcaggttt attcatagac gatgccggcg agcatttcct gttcgttcaa gttgccgtac
     2401 tctttttccc agtcgtgtga agactcgatg atgtcgcatt ctttggaaag ggagacttgt
     2461 tctgcatcca tatctttggc gttcagtatg ttgaattttt cgcacaggga tgcggataaa
     2521 gtgatgtcag gctgtttggc ttcagaacgg ttttcttgga aggcaaagca gaatgcggta
     2581 aatgccgcag catagataag atatttgccg gttttcttca ttttctatcc tttttctgtc
     2641 aactcaggat taaacctatg gaaaatctta aaaattatgt attgagtaag aaaaatcata
     2701 atttaaattt agtttatcat agttgttccg tttttgggat agctaaggta aaatatattt
     2761 catgtttact ttagatgatt gaatgaaggg gagcggaagg atatttatgg aaacctttaa
     2821 agacagactg gtttttttat ggaaaagcga agcgaagcag gcaaaaatcg catccgatat
     2881 tgaaatgacg attgcgggct tcagcaggat atggaatgaa ggcggtctgc cgaagtctga
     2941 aacattaaaa aaaatcaagc agttgaaggg gtgtagtatc gattggctgc tgaccgggga
     3001 gggtaatccg tttccggatg aagccccaaa aaaatccctt gcttacgata ccttgggcaa
     3061 tgaagttgat acggacgagt ttgtctttgt gccgagatat gatattcggg cggctgcggg
     3121 atacgggcag tttgtcgatc atgaggaacc ggtatttaca atggcgttca gacggcattg
     3181 gattgagaat tatgttaccc gcgatacgaa aaacctgtct gtaatttccg tcaaggggga
     3241 ttctatggag ggggttttga atgacggcga ttcgattttg gtcaatcatg gtgaaaatac
     3301 gccgagggac ggtctgtatg tgttgcggat taatgaaaat ctgctggtta aacgtttaca
     3361 gattgtaccg ggcgggatta tcaatgtgat ttctgcaaac gaggcttatc ctgcttttga
     3421 aatcaatttg aacgatttga ccgatgatgt ggagattatc gggcgtgtcg agtggttcgg
     3481 caggacgatt tgagtttggg gcttgaaatt gcaggcggtc aaacttatct attggaacaa
     3541 ttcctttttc aaaggcgaag cctgcttgcc tttaaagggg gtttgagaga gaatgcagaa
     3601 aatattatat taaggaatca caccatgtcg gatgaaagcc ctattatttt tactgacagc
     3661 tgctgtgcca aagttgccga tttgattgcc gaagaaaaca atcccgattt gaaattgcgg
     3721 gtttttgtca atggcggcgg ctgttcgggt ttccagtacg gatttacttt tgacgaaatc
     3781 aaaaacgacg acgattttga aattgagaaa aacggtttgg tctttttggt cgatccgatg
     3841 agctatcaat atctggtcgg tgcggaaatc gactatacgg aaagtttgca gggttcgcaa
     3901 ttcgtcatcc gcaatccgaa tgcggaaaca acctgcggtt gcggatcgtc gttttccgta
     3961 tgaccgcttg gtttgtgtga tgccgtctga acgttcagac ggcattttta cttttggaaa
     4021 atatattatc gggatgaatt cacatataat ccgattgttt gaagatgaat cgggtttccc
     4081 gaaaggaacg ggcggaacgg tatcaggcgt atttgttccc ttatgattga gatgagtaaa
     4141 gattaccgaa atgatttgta cgatgtatat gtttcctacc cgccccaagt ggatcgcggg
     4201 cttatccggg agtgcctcag ggagaatctc ggcgaggaaa aggcggaagg attgatcgaa
     4261 tcgctcgatt ccaaacctca agtgctggtt gaggaaaaat gcacttgggc gaaacgggaa
     4321 gagttgcacg attatttcag ctatttgggt ttggatatta ttacccggag atatagtgga
     4381 ttaacaaaaa ccagtacggc gttgtctcgc cttagctcaa agagaacgat tctctaaggt
     4441 gctgaagcac caagtgaatc ggttccgtac tatctgtact gtctgcggct tcgtcgtctt
     4501 gtcctgattt ttgttaatcc actacatatg gagttagaaa cggtcgtgcc gccggaggaa
     4561 ggtgagggcg aaggagaggg cgcggatggg gaaatgcccg aatatcttga acttcatggc
     4621 gggcgggaag atgatatttc cgcaccttcg caacccgaac cgccgacccg caatatcaaa
     4681 ccgctgcttt tcgggctgct gattgccttt ttgggctatc tgctcggtaa gattttttga
     4741 ttgtccgata aatgctgtat tcgggatttt atatatgaaa tggttgaaac gcctgacggt
     4801 cattgtcggg actttttacc gctatcggct ggcaggtctg tgtgcttcgc tgatgggtag
     4861 cggttggata tgcgctctgc tgaaaatgat gccgcagtcg tccaaattga aaaacgaacc
     4921 gcctgccgtc cgtctgcgcc ttgccttgga aagcctgggg ccgattttca tcaagttcgg
     4981 gcaggttttg tccacacgcc ccgatttgat tccgcacgat tacgccgtcg aactggcaaa
     5041 gttgcaagac aaagtgccgc cttttgacgc gcggctttcg cgtgaacaaa tcgaaaaatc
     5101 gctgggccag cccatcgaaa agctgtatgc ggaatttgaa accgagcccg tcgccagcgc
     5161 gtccatcgcc caagtacaca aagcccgcct gcattcgggc gaacaagtgg cggtcaaagt
     5221 cttgcgcccc aaccttttgc ccgtgatcga acaggatttg tcgctgatgc gctttggtgc
     5281 aggctgggtc gagcgtctgt ttgccgacgg caagcgtctg aagccgcgcg aagtggtggc
     5341 ggagttcgac aaatatctgc acgacgagtt ggacttgatg cgcgaagccg ccaatgccag
     5401 ccagctcgga cgcaatttcc aaaacagcga tatgctgatt gtgccgaagg tgttttacga
     5461 ctactgcacc agcgacgtgc tgaccatcga atggatggac ggcacgccgg tttccgacat
     5521 cgccaaactc aaagcagacg gcatcgattt gcacaaactc gccgattacg gcgtggaaat
     5581 ctttttcacg caggtcttcc gcgacggctt tttccacgcg gatatgcacc ccggcaatat
     5641 tttggttgcc gccgacaacc gctacatcgc cctcgatttc ggcatcgtcg gcacgctgac
     5701 cgattacgac aaacgttatc tcgccatcaa tttcctcgcc tttttcaacc gcgattaccg
     5761 gcgcgtcgcc accgcccaca tcgaatcggg ctgggtgcct gccgacacgc gtgcggaaga
     5821 attggaagcc gccgtccgcg ccgtgtgcga accggtgttc aacaaaccga tttcgcagat
     5881 ttccttcggc ttggtgctga tgcgcctgtt tgaagtcagc cgccgcttca atgtcgaaat
     5941 ccaaccgcag cttgtgctgc tgcaaaaaac gctgctcaac atcgaaggct tgggccgcca
     6001 gctcgatccc gatttggact tgtggaaaac cgccaaaccg tttttggtga aatggataaa
     6061 cgggcaggtc ggccctaaag ccctttggcg caacctcaaa aacgaagccc ccgactgggc
     6121 gcaaatcatt ccttcattgc cgcgcaaaat cagtgcgttg attgatgaaa accgccagca
     6181 ggaaatgcgt gatgcctata tccatttggt caaagtgcag cagcggcaaa gcctgtggct
     6241 ggctgtgatt gcggttgttt tgctgctgat tttgcttttg aaataggctt tgtccgaatc
     6301 atcgcccgac tccgcccgtt tataaggaaa tcg
//
LOCUS       DAWFHY010000100         6168 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.100, whole genome shotgun sequence.
ACCESSION   DAWFHY010000100 DAWFHY010000000
VERSION     DAWFHY010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6168
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.100"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            144..1541
                     /gene="aspA"
                     /locus_tag="WGQ68_001831"
     CDS             144..1541
                     /gene="aspA"
                     /locus_tag="WGQ68_001831"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009311384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEZ0715593.1"
                     /translation="MTVRIEHDLLGDREIPAEVYWGIHTLRAIENFKISTQKISDVPQ
                     FVRSMVMVKKATAQANGELGAVKPEIAAAIEKACDEVLLNNRCLDQFPSDVYQGGAGT
                     SVNMNTNEVIANLALEALGYEKGRYDIVNPMDHVNASQSTNDAYPTGFRLAVYYSIGE
                     LLDKLTVLKNAFTAKAEEFKDVLKMGRTQLQDAVPMTAGQEFQSFQVLLEEEILNLDR
                     TRQLLLEVNLGATAIGTGVNTPKGYAELVVKKLSEVSGLPCKLTENLIEATSDCGAYV
                     MVHGALKRTAVKLSKICNDLRLLSSGPRAGLKEINLPELQAGSSIMPAKVNPVIPEVV
                     NQVCFKVIGNDTTITFAAEAGQLQLNVMEPVIAQCMFETISLLGNAAINLSDKCVKGI
                     TVNREICERYVFNSIGLVTYLNPYIGHHNGDLVGKICAQTGKGVREVVLERGLLSEEE
                     LNRILSPENLMNPHL"
     gene            complement(1613..2488)
                     /locus_tag="WGQ68_001832"
     CDS             complement(1613..2488)
                     /locus_tag="WGQ68_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEZ0715594.1"
                     /translation="MAYLLISIVFSVSVSILLKMARKKKIDIAQAVAVNYVVAVILTL
                     LVLKPDVGNIGAFLPTWPLFAALGVLLPSVFVIMGKSVESAGIVKSDAAQRLSLFLPI
                     VAALTLFGEKLSEGKLIGLCLAFAALFCLLWKHSGGKKSGSAWRQAALLLGVWAGYGI
                     IDILFKQLAKSGTAFAGNLLVAFVLAGVLMFACLFAKSVRWRAESVVGGILLGGLNFM
                     NIVTYITAHQMMKDNPTLVFAGMNIGVIVLGTLSGALFFKEKINTINTAGIVLALCSI
                     ACLFYWGEVKVLFGI"
     gene            complement(2490..3214)
                     /locus_tag="WGQ68_001833"
                     /pseudo
     CDS             complement(2490..3214)
                     /locus_tag="WGQ68_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
     gene            complement(3236..3715)
                     /gene="ybaK"
                     /locus_tag="WGQ68_001834"
     CDS             complement(3236..3715)
                     /gene="ybaK"
                     /locus_tag="WGQ68_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEZ0715595.1"
                     /translation="MSKHTYPITPAVRVLRENGIEFEPFTYAYEEHGGTAQFARLFGK
                     DEHLVIKTIVLQDENGKGLIVLMHGDKQISTRNLARHLGAKHIEPATPAQANKWTGYL
                     VGGTTPFGIRTKLDIYVEQSVMDLDIIYINGGKRGFIIGIRPGDLNILNPKTIQAAV"
     gene            3790..4152
                     /locus_tag="WGQ68_001835"
     CDS             3790..4152
                     /locus_tag="WGQ68_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019273361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEZ0715596.1"
                     /translation="MDIRYFGTTPRYSEAVGANGLIFLSGMVPENGETAAEQTADVLA
                     QIDRWLAECGSDKAHVLDAVIYLRDMGDYAEMNGVWDAWVAAGRTPARACVEARLARP
                     EWRVEIKITAMKKDAATA"
     gene            4187..5050
                     /locus_tag="WGQ68_001836"
     CDS             4187..5050
                     /locus_tag="WGQ68_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein YgfZ"
                     /protein_id="HEZ0715597.1"
                     /translation="MKTLLPFFGVVRVSGEDRQTFLHGQLSNDINNLQTGQACYATYN
                     TPKGRVIANMIVVNRGGDLLLIMAQDLLEATVKRLRMFVLRAKAVFEILEDYAVDAEL
                     AASAEPLAAQEPSLVFTAECVSDGICTVVLPHRGILHIAPKNALPPYDAAAESAWRLH
                     EIRSGYPWICAATKETAVAQMLNQHIIGGVHFKKGCYPGQEIIARAQYRGQVKRGLAV
                     LSGNSAAEVGSVLAADGEEAGIVLDSVKDSENFTALAVIKFSAAQKTLSAPNGGSFKA
                     VHLFFKTENAE"
     gene            5148..6107
                     /gene="ttcA"
                     /locus_tag="WGQ68_001837"
     CDS             5148..6107
                     /gene="ttcA"
                     /locus_tag="WGQ68_001837"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HEZ0715598.1"
                     /translation="MSKKTKQELENNKLSKRLRHAVGDAINDFNMIEPGDKIMVCLSG
                     GKDSYALLDILRQLQASAPIDFELVAVNLDQKQPGFPEEVLPTYLESIGVPYKIVEED
                     TYSTVKRVLDEGKTTCSLCSRLRRGILYRTAKELGCTKIALGHHRDDILATMFLNMFY
                     GGKLKAMPPKLVSDNGEHIVIRPLAYVKEKDLIKYAELKQFPIIPCNLCGSQPNLQRQ
                     VIGDMLRDWDKRFPGRIESMFSALQNVVPSHLADTELFDFAGLERGQTLKHGGDLAFD
                     SEKMPERFSDGSEEDESEIKIAPQKAERKVINILANKPKTCGA"
BASE COUNT         1566 a         1777 c         1498 g         1327 t
ORIGIN      
        1 cacccgaaaa caacgtttcc cgttttcaga cggcatacga tatggacaat ttcatccggc
       61 cggtatagtg ttcgtcctgc tgcggcatct ttccgatggc gcattttttc aatattaatt
      121 atccgactta caaaggtaaa aacatgactg tccgtatcga acacgattta ttgggcgacc
      181 gcgagattcc cgccgaagtg tattggggca tccacactct gcgcgccatt gaaaacttta
      241 aaatctccac acaaaaaatt tccgacgtgc cgcagtttgt ccgcagtatg gtgatggtga
      301 aaaaagcaac cgcgcaggca aacggcgaat tgggtgcagt aaaacccgaa atcgccgccg
      361 ccattgaaaa ggcttgcgac gaagttctgc tgaacaaccg ctgcctcgac caattcccct
      421 ccgacgtgta tcagggcggt gcgggaactt cggtcaatat gaacaccaac gaagtcatcg
      481 ccaacctcgc attggaagcc ttgggatacg aaaaaggccg ctacgacatc gtcaatccga
      541 tggatcatgt caatgccagc caatcgacca acgatgccta tcccacgggc ttccgccttg
      601 ccgtgtatta cagcatcggc gaattgctcg acaaactgac cgtcctgaaa aacgccttta
      661 ccgccaaagc cgaagaattt aaagacgttt tgaaaatggg tcgcacccag cttcaagatg
      721 ccgtaccgat gacggcagga caagaattcc aatctttcca agtgttgttg gaagaagaaa
      781 tcctcaacct cgaccgcacg cgccaactgc ttttagaagt caatttgggc gcaacggcaa
      841 tcggcacggg cgtgaacacg cccaaaggct acgccgaact ggtggtcaaa aaactctccg
      901 aagtcagcgg cttgccttgc aaactgaccg aaaacctgat tgaggcgacc tccgactgcg
      961 gcgcatatgt gatggtacac ggcgcattga agcgcacggc ggtcaaactc tccaaaatct
     1021 gcaacgactt gcgcctgctc tcttccggcc cgcgtgccgg cttgaaagaa atcaacctgc
     1081 ccgaattaca ggccggttct tccattatgc ctgccaaagt caatcccgtg attcccgaag
     1141 tggtcaacca agtctgcttc aaagtcatcg gcaacgatac gacgattacc ttcgccgccg
     1201 aagccgggca actgcaatta aacgtgatgg agccggtcat cgcccaatgt atgtttgaaa
     1261 ccatttccct cttgggcaat gcagcaatca acctgtccga caaatgcgtc aaaggcatta
     1321 cggtcaaccg cgaaatatgc gagcgttacg ttttcaactc catcgggctg gttacttatc
     1381 tgaatccgta tatcggacac cacaacggcg atttggtcgg caaaatctgc gcccaaaccg
     1441 gcaaaggcgt gcgcgaggtt gtcttggaac gcgggctgtt gagcgaagaa gaactcaacc
     1501 gcatcctctc tcctgaaaac ctgatgaacc cccatctgta acgttgcaga caaatgccgt
     1561 ctgaagcagg gatgctgctt cagacggcat tttgttatat caatcagccg gattatatgc
     1621 cgaacagtac cttgacttcc ccccaataaa acaggcaggc gatagaacac agtgccaaca
     1681 cgattcccgc cgtattgatt gtgttgattt tttccttaaa aaacaatgcg cccgaaagcg
     1741 tacccaaaac aatcacgccg atattcatac cggcaaaaac caaggtcgga ttgtccttca
     1801 tcatttggtg cgcggtgatg taggttacga tattcataaa attcaaaccg cccaagagta
     1861 tgccgccgac cacactctct gcgcgccatc tgaccgattt ggcaaacagg caggcaaaca
     1921 tcagcacacc cgccagcaca aatgcaacca gcaggttgcc cgcaaatgcc gttccgcttt
     1981 tggcaagctg tttgaacagg atatcgataa tgccgtaacc tgcccacacg cccagcagca
     2041 atgccgcctg ccgccacgcg cttcctgatt ttttgccacc gctgtgtttc caaagcaggc
     2101 agaacagtgc ggcaaaggcg aggcacagcc cgattagttt gccttcgctg agtttttcgc
     2161 caaacagcgt taaagcggca acaatcggca aaaacagcga caaacgctgc gccgcgtcgg
     2221 atttgacgat accggcggat tctacggatt tgcccattat cacgaatacg gacggcagca
     2281 gcacgcctaa tgcggcaaac agcggccacg tcggcaaaaa tgcgccgata ttgccgacat
     2341 ccggcttcaa taccagcagg gtcagtatga ccgcgaccac ataattgacg gcgaccgcct
     2401 gcgcgatgtc gatttttttc ttccttgcca ttttcagcaa aatggaaacc gacacgctga
     2461 acacgatgct gattaacagg taagccatac tatatccatt tctcgggtgc aaccgccctg
     2521 ccgctgttga agcggataaa gccgacgatg atgcggtaga aataccagac gttcgttgca
     2581 ataataatca gcacgccgat acccaaaagg gcagtcagcg cgcccaaaag ataaagccaa
     2641 aatgtgcgcc aaaaggtttt aatcaggtat tcggtatgtg tggcatagac aatgctgtcc
     2701 aaactgttcc gtttcatata ggcgagaatc acgccgacta tgggcatata ggcgaaaatc
     2761 acgccaacta tgggcatgaa cagcatgatt gcacccaaac cgaacaggat gtaggcggcg
     2821 aggacatagt tgcgcccgct tccgccgccg gacggttcgt aagcatggcg cgaacgtgag
     2881 gcctgccgtt caaacgcctg tctgaacgcc tgttcgcgcg gcgcatctgt tcgcggtaaa
     2941 actgctccct gcgcgcctgt tcgcggcgga atgcctcttc ctgccgcccg cgttcctcat
     3001 gccgtctgaa ggacgcgtcg tattgcatcc gtttcgacag gtcggaaagc gtatcgtaag
     3061 cccgttggat ttctttgaac ttttcttccg cctgcggatt gcccggattg cggtcgggat
     3121 ggtatttcat cgccagcttg cggtaggcgc gtttgatttc gttcgcaccc gcctgcggcg
     3181 acacgcccaa tacggcatac aaatctttgt ccatattgct ccttatactt cccggtcaaa
     3241 ccgccgcctg tattgttttc gggttcaaaa tatttaaatc tccgggacgg atgccgataa
     3301 tgaacccgcg ttttccgccg ttgatataga tgatgtccaa atccatcacc gactgttcga
     3361 cataaatatc caattttgtc cggatgccga acggcgttgt gccgccgacc agatagcccg
     3421 tccacttgtt tgcctgtgct ggcgtggcgg gttcgatgtg tttcgcaccc aaatgccgcg
     3481 ccagattgcg ggttgaaatc tgcttgtcgc cgtgcatcaa gacaatcagc cccttaccgt
     3541 tttcatcttg caaaacaatg gttttaatga ccaagtgttc gtctttgccg aatagtcggg
     3601 caaactgcgc cgtgccgccg tgttcctcat aggcataggt aaaaggttca aattcgatgc
     3661 cgttttcacg caaaacgcgc acggcaggtg ttatcggata agtgtgttta ctcatttttc
     3721 gtcatgcaaa acatggaaat cggtcaagcg caatgttacc atatatcccc tttatcgaag
     3781 gaaaccgata tggatatccg atatttcggc acaacgcccc gctattccga agccgtcggc
     3841 gcaaacggct tgattttcct ctccggcatg gttcccgaaa acggtgaaac ggctgccgaa
     3901 cagactgccg acgtgcttgc ccaaatcgac cgctggctgg cggaatgcgg ctcggacaaa
     3961 gcgcacgttt tggatgcggt catctatctg cgcgatatgg gcgactatgc ggaaatgaac
     4021 ggcgtgtggg acgcttgggt tgccgccggc agaacgcccg cccgcgcctg cgtggaagct
     4081 cggctcgccc gtccggagtg gcgcgtcgaa atcaaaataa ccgccatgaa gaaagatgcg
     4141 gcaaccgcat aaacagattt cctttatatt tcagagtacg caaatgatga aaaccttact
     4201 gccctttttc ggcgtggtgc gcgtcagcgg cgaagaccgc caaaccttcc tgcacggaca
     4261 gctttccaac gacatcaata atcttcagac cgggcaggcg tgttatgcga cttacaacac
     4321 gcccaaaggc cgcgtcatcg ccaatatgat tgtcgtcaac cgaggcggcg atttgctgct
     4381 gattatggcg caagacttgc ttgaagcaac ggtcaaacgt ttgcggatgt ttgtgttacg
     4441 cgccaaagcc gtttttgaaa tccttgaaga ttacgccgtc gacgcggaat tggcagcatc
     4501 cgccgaaccg cttgccgcgc aagaacccag tctcgtcttt accgccgaat gcgtttcaga
     4561 cggcatctgc accgtcgtcc tgccccatcg gggcattttg cacatcgccc ccaaaaacgc
     4621 cctgccccct tatgatgccg ccgccgaaag cgcgtggcgg ctgcacgaaa tccgcagcgg
     4681 ttatccgtgg atatgcgcgg ctaccaaaga aaccgccgtc gcccaaatgc tcaaccagca
     4741 catcatcggc ggcgtacact tcaaaaaagg ctgctatccg ggacaggaaa tcatcgcgcg
     4801 tgcccagtat cgggggcagg tcaaacgcgg cttggcggta ttgtcgggaa attcggcagc
     4861 cgaagtgggt agcgtattgg cagcggacgg cgaagaggcc ggcatcgtgc ttgacagcgt
     4921 aaaagattcg gaaaacttta ccgcccttgc cgtaattaaa ttctccgccg ctcaaaaaac
     4981 actgtccgcc ccaaacggcg gcagcttcaa agcggttcat ctcttcttta aaaccgaaaa
     5041 tgcagaataa gccctctaat gccgtctgaa cctttttcag acggcatttt aaaaccgccc
     5101 ctgttttcgg ttacaatgcc gcatccctaa tttttcaaac caccaccatg tccaaaaaaa
     5161 ccaaacaaga actcgaaaac aacaaactca gcaaacgcct gcgccacgcc gtcggcgacg
     5221 caattaacga tttcaacatg atcgagccgg gcgacaaaat catggtctgc ctctccggcg
     5281 gcaaagacag ctacgccctg ttagacatcc tgcgccagct ccaagccagc gcgccgattg
     5341 atttcgaact ggttgccgtc aatctcgacc aaaagcagcc gggcttcccc gaagaagtat
     5401 tgccgaccta tctcgaaagc ataggcgtgc cgtacaaaat cgtcgaagaa gacacctact
     5461 ccaccgtcaa acgcgtattg gacgaaggca aaacgacttg ttcactgtgc agtcgcctgc
     5521 gccgcggcat cctctaccgc accgcaaaag aattaggctg caccaaaatc gccttgggac
     5581 accaccgcga cgacatcctc gctaccatgt ttttaaatat gttttacggc ggcaaactca
     5641 aagccatgcc gcccaagctg gtgagcgaca acggcgaaca catcgtcatc cgtccgctgg
     5701 cgtatgtcaa agaaaaagac ttaatcaaat acgccgaatt gaaacagttc ccgattatcc
     5761 cgtgcaacct ctgcggctcg cagcccaacc tgcaacgtca agtcatcggc gatatgctgc
     5821 gcgattggga caaacgcttc cccggccgta tcgaatcgat gttctccgcc ctgcaaaacg
     5881 tcgtcccttc ccaccttgcc gataccgaac tcttcgactt tgccggattg gaacgcggtc
     5941 aaaccctgaa acacggcggc gatttggcgt ttgacagcga aaaaatgccc gaacgttttt
     6001 cagacggcag cgaagaagac gaaagcgaaa tcaaaatcgc gccgcaaaaa gccgaacgca
     6061 aagtcatcaa cattctggcg aacaagccga aaacctgcgg cgcgtaaccc cccgccgtca
     6121 aaagtgtaga ttgcctcggt gttcgccccg gctgtgtcgc ttagatca
//
LOCUS       DAWFHY010000101         6101 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.101, whole genome shotgun sequence.
ACCESSION   DAWFHY010000101 DAWFHY010000000
VERSION     DAWFHY010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 6101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6101
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.101"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..183)
                     /locus_tag="WGQ68_001838"
     CDS             complement(<1..183)
                     /locus_tag="WGQ68_001838"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715599.1"
                     /translation="MPSELQTAFPASQLQRERTHTAIPAKNRKSKKKPKIRHSRAGGN
                     PIRPVSVFFEFQVTSKP"
     gene            complement(196..780)
                     /gene="rnhB"
                     /locus_tag="WGQ68_001839"
     CDS             complement(196..780)
                     /gene="rnhB"
                     /locus_tag="WGQ68_001839"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEZ0715600.1"
                     /translation="MHILTAGVDEAGRGPLVGSVFAAAVILPETFDLPGLTDSKKLSE
                     KKRDALAEMIKNQAAAWHVAAATPEEIASLNILHATMLAMKRAVDGLAVRPERIFIDG
                     NRIPEHLGIPAEAVVKGDSKIIEISAASVLAKTARDAEMYALAQRHPQYGFDKHKGYG
                     TKQHLEALKQYGVLPEHRRDFAPVRNLLAQQTLF"
     gene            complement(849..2744)
                     /gene="mnmG"
                     /locus_tag="WGQ68_001840"
     CDS             complement(849..2744)
                     /gene="mnmG"
                     /locus_tag="WGQ68_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEZ0715601.1"
                     /translation="MTHMIYPKTYDVIVVGGGHAGTEAALAAARMGAQTLLLTHNIET
                     LGQMSCNPSIGGIGKGHLVRELDALGGAMALATDKSGIQFRRLNASKGAAVRATRAQA
                     DRILYKAAIREMLENQENLDLFQQAVEDVTLDGERISGVITAMGVEFKARAVVLTAGT
                     FLSGKIHIGLENYEGGRAGDPAAKSLGGRLRELKLPQGRLKTGTPPRIDGRTIDFSQL
                     TEQPGDTPVPVMSVRGNAEMHPRQVSCWITHTNTQTHDIIRSGFDRSPMFTGKIEGVG
                     PRYCPSIEDKINRFADKDSHQIFLEPEGLTTHEYYPNGISTSLPFDIQIALVRSMKGL
                     ENAHILRPGYAIEYDYFDPRNLKASLETKTIQGLFFAGQINGTTGYEEAAAQGLLAGA
                     NAVQYVREQDPLLLRREQAYLGVLVDDLITKGVNEPYRMFTSRAEYRLQLREDNADMR
                     LTEDGYKIGLVGEAQWRMFNEKREAIEREIQRLKTTWYTPQKLAEDEQIRVFGQKLSR
                     EANLHDLLRRPNLDYAALMTLEGAMPSENLSAEVVEQVEIQVKYQGYIDRQNEEIDSR
                     RDIETLKLPEDIDYSKVKGLSAEVQQKLNQHKPETVGQASRISGVTPAAVALLMVHLK
                     RGFKDAK"
     gene            complement(3069..4496)
                     /locus_tag="WGQ68_001841"
     CDS             complement(3069..4496)
                     /locus_tag="WGQ68_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEZ0715602.1"
                     /translation="MNENFTEWLHGWVGAINDPMWSYLVYMLLGTGLFFTVTTGFVQF
                     RLFGRSIKEMLGGRKQGDDPHGITPFQAFVTGLASRVGVGNIAGVAIAIKVGGPGAVF
                     WMWVTALIGMSSAFVESSLAQLFKVRDYDNHHFRGGPAYYITQGLGQKWLGVLFALSL
                     IFCFGFVFEAVQTNTIADTVKAAWGWEPHYVGVALVILTAPIIFGGIRRISKAAEIVV
                     PLMAVLYLFIALFIILTNIPMIPDVFGQIFSGAFKFDAAAGGLLGGLISQTMMMGIKR
                     GLYSNEAGMGSAPNAAAAAEVKHPVSQGMIQMLGVFVDTIIVCSCTAFIILIYQQPYG
                     DLSGAALTQAAIVSQVGQWGAGFLAVILFMFAFSTVIGNYAYAESNVQFIKSHWLITA
                     VFRMLVLAWVYFGAVANVPLVWDMADMAMGIMAWINLVAILLLSPLAFMLLRDYTAKL
                     KMGKDPEFKLSEHPGLKRRIKSDVW"
     gene            4656..5645
                     /gene="pdxA"
                     /locus_tag="WGQ68_001842"
     CDS             4656..5645
                     /gene="pdxA"
                     /locus_tag="WGQ68_001842"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEZ0715603.1"
                     /translation="MKQPVFAVTSGEPAGIGPDICLDLAFARLPCRCAVLGDKNLLRA
                     RAEALGKSVVLRDFDPESGGAAYGELEVLHIPAAEAVEAGRLNPANAAYVLQLLDTAL
                     AGISDGIFDGIVTAPLHKGIINDARASTGFFSGHTEYLAEKSGTGQVVMMLAGKGLRV
                     ALVTTHLPLKDVAAAITQPLIESVARILHHDLKHKFGIKNPKILVAGLNPHAGEGGHL
                     GHEETDTIIPALENLRREGINLAGPYPADTLFQPFMLEGADAVLAMYHDQGLPVLKYH
                     SFGQGVNITLGLPFIRTSVDHGTALDLAATGRADSGSLITAVETAVEMARGSL"
BASE COUNT         1270 a         1612 c         1790 g         1429 t
ORIGIN      
        1 cggtttggaa gttacctgaa attcaaaaaa aacggaaacc ggacggattg gattcccgcc
       61 tgcgcgggaa tgacggattt taggtttctt ttttgatttt ctgtttttcg cgggaatggc
      121 ggtatgggtt ctttctcttt gaagttgcga tgctggaaat gccgtctgaa gttcagacgg
      181 catttttgtg ccggtttaaa acagcgtctg ctgcgcgagc aggtttctga cgggggcgaa
      241 gtcgcggcga tgttcgggca gcacgccgta ttgtttgagg gcttccagat gctgcttcgt
      301 gccgtaacct ttgtgtttgt cgaaaccgta ttggggatgg cgttgcgcca gtgcgtacat
      361 ttccgcatcg cgtgcggtct ttgccaaaac ggatgcggcg gagatttcga tgattttgct
      421 gtcgcctttg acgacggctt cggcagggat acctaaatgt tctggaatgc ggttgccgtc
      481 gatgaatatt ctttcgggac gcacagccaa accgtcaacg gcgcgtttca tggcgagcat
      541 ggtagcgtgc aggatgttga ggctggcgat ttcttcgggc gtggcggcgg cgacgtgcca
      601 cgcggccgcc tgatttttta tcatttcggc aagcgcgtcg cgttttttct cgctgagttt
      661 tttggagtcg gtcagtccgg gcaggtcgaa tgtttccgga aggatgacgg cggcggcaaa
      721 cacgctgccg actaaaggcc cgcgtcctgc ctcgtccacg ccggcggtca gtatgtgcat
      781 gatgtttcct gtcgggatag taggaatgcc gtctgaaaag ggtttcagac ggcatcgtac
      841 cgatgtgttt atttcgcgtc tttaaacccg cgcttcaaat gcaccatcag caatgccact
      901 gccgcagggg ttacgccgga aatgcggctt gcctgcccga cggtttcggg tttgtgctgg
      961 ttgagctttt gctgcacttc tgccgacaaa cctttaactt tgctgtaatc gatatcctcg
     1021 ggcagcttta aggtttcgat gtcgcggcgg ctgtcgattt cttcgttttg gcggtcgata
     1081 tagccttggt atttgacttg gatttcgact tgttcgacca cttcggcgga gaggttttca
     1141 gacggcatcg cgccttcgag cgtcatcagc gcggcgtagt cgaggtttgg gcggcgcagg
     1201 aggtcgtgca ggttggcttc gcggctgagc ttttgcccga atacgcggat ttgttcgtct
     1261 tcggcgagtt tttgcggcgt gtaccacgtt gttttcaaac gttggatttc gcgttcgatg
     1321 gcttcgcgtt tttcgttgaa catgcgccat tgcgcttcac ccaccaagcc gattttgtag
     1381 ccgtcttcgg tcaggcgcat atcggcgttg tcttccctga gttgcaggcg gtattcggca
     1441 cggctggtga acattcggta gggttcgttc acgcctttgg tgatgaggtc gtccaccaat
     1501 acgccgagat aggcttgttc gcggcgcagc aggagcgggt cttgttcgcg gacgtattgc
     1561 acggcgttcg cgcctgccag cagtccttgc gcggcggctt cttcgtagcc cgttgtgccg
     1621 ttgatttgtc cggcgaagaa gaggccttgg atggttttgg tttcgaggct ggctttgagg
     1681 ttgcgcgggt cgaagtagtc gtattcgatg gcgtagccgg ggcgcaggat atgggcgttt
     1741 tccaaacctt tcatactgcg gacgagcgcg atttggatgt cgaacggcag gctggtggag
     1801 ataccgttag gatagtattc gtgcgtggtc agaccttcgg gttcgaggaa aatctggtgg
     1861 ctgtctttgt cggcgaagcg gttgattttg tcttcgatag acggacaata acgcggaccc
     1921 acgccttcga ttttgccggt aaacatcggg ctgcggtcga agcctgagcg gatgatgtcg
     1981 tgggtttgcg tgttggtatg cgtaatccag caggacactt ggcgcgggtg catttcggcg
     2041 ttgccgcgca cggacatgac gggaacgggt gtgtcgccgg gctgttcggt cagttgggaa
     2101 aagtcaatcg tgcgtccgtc gatgcgcggc ggcgtgccgg ttttcagacg gccttgcggc
     2161 agcttcaatt cgcgcaaacg tccgcccagc gatttggcgg cggggtcgcc ggcgcgtccg
     2221 ccctcgtagt tttccaaacc gatgtggatt ttgccggaca aaaacgtgcc tgcggtcagc
     2281 accacggcgc gtgctttaaa ctccacgccc atcgcggtga ttacgccgct gatgcgttcg
     2341 ccgtcgagcg ttacgtcttc gacggcttgt tggaaaaggt cgaggttttc ttggttttcc
     2401 aacatttcgc ggatggcggc tttgtacaaa atgcggtcgg cctgcgcgcg cgtggctcga
     2461 actgccgcgc ctttgctggc gttcaggcgg cggaactgga taccggattt gtcggtggcc
     2521 aacgccatcg cgccgccgag cgcgtcgagt tcgcgcacca aatgcccttt gccgatgccg
     2581 ccgatggaag ggttgcacga catttgtccg agcgtttcaa tattgtgtgt gagcaaaagc
     2641 gtctgcgcgc ccatacgggc ggcggcgagc gcggcttctg tgccggcgtg tccgccgccg
     2701 acgacgataa cgtcgtaggt tttggggtaa atcatgtggg tcatgtgtgt attgcctgtc
     2761 ggtgtttcag acggcattta tagtggatta acaaaaacca gtacagcgtt gcctcgcctt
     2821 agctcaaaga gaacgattct ctaaggtgct gaagtaccaa gtgaatcggt ttcgtactgc
     2881 ttgtactgtc tgcggcttcg tcgccttgtc ctgatttttg ttgaaccact atattcaata
     2941 tgccgtctga aaaacgaaat ggattcaaaa gtaaagggtt gggattgtac gcttgttcgc
     3001 cctgttttta cagtgtgcgg aaagggaaaa gccgcttcgc ggggaagcgg ctccggtaag
     3061 ggcgggattt accaaacgtc ggatttgata cggcgtttca ggcccggatg ttcggaaagt
     3121 ttgaactcgg ggtctttgcc cattttcagc ttggcggtgt aatcgcgcag cagcataaac
     3181 gccaagggcg agagcagcag gatggcgaca aggttgatcc acgccataat gcccatcgcc
     3241 atatccgcca tatcccagac caaaggcaca ttggcaaccg cgccgaaata gacccacgcc
     3301 aaaaccagca tacggaaaac ggcggtaatc agccaatggc ttttgatgaa ttggacgttg
     3361 gactcggcat aggcatagtt gccgataacg gtggaaaagg caaacataaa caggatgacg
     3421 gcgaggaagc ccgcgcccca ttgccccact tggctgacaa tcgccgcctg cgtcagcgcc
     3481 gcaccgctca aatcgccgta aggctgttgg taaatcaaga tgatgaaggc ggtgcaagaa
     3541 caaacgatga tggtatcgac aaacacgccc agcatttgaa tcataccttg cgaaacaggg
     3601 tgtttcactt cggcggcggc ggcggcgttc ggcgcggaac ccatacccgc ctcgttggaa
     3661 tacaggccgc gtttgatgcc catcatcatc gtttgcgaaa tcagaccgcc gagtaagccg
     3721 cctgctgccg cgtcgaattt gaacgcgccc gaaaaaatct gaccgaacac gtccggaatc
     3781 atcggaatat tggtcaaaat gatgaaaagc gcgataaaga ggtacaaaac cgccatcagg
     3841 gggacgacga tttccgccgc tttagatatg cgcctgatgc cgccgaagat aatcggcgcg
     3901 gttaaaatca ccagggcgac gccgacataa tgaggctccc aaccccacgc cgctttgacg
     3961 gtatcggcga tggtattggt ctgaaccgct tcaaacacaa agccgaaaca gaaaatcagg
     4021 ctcagggcga acaacacgcc cagccatttc tgccccagcc cttgagtgat gtagtaggca
     4081 gggccgcccc ggaaatggtg gttgtcgtag tcgcggactt taaagagctg cgccagcgaa
     4141 gattcgacaa acgccgaact cataccgatt aaggcggtta cccacatcca aaacaccgcg
     4201 cccggcccgc cgactttgat ggcgatggcc acgcccgcga tattgcccac gcccacgcgg
     4261 ctggcaaggc cggttacaaa tgcctgaaac ggcgtgatgc cgtgagggtc gtccccctgt
     4321 ttgcggcctc cgagcatttc tttgatgctg cgcccgaaca ggcggaattg gacaaagccc
     4381 gtggttacgg tgaagaaaag ccccgtaccc aaaagcatat aaaccaagta tgaccacatc
     4441 ggatcgttga tggcgccgac ccagccgtgc agccattcgg taaagttctc gttcatatcg
     4501 cttccttaaa gttgaaaccc gcacatattg gcggtatgca agcagggttt aaattttgta
     4561 aacgcccatt ctagcagatt gtcaacaaaa tcagaaaaat ttacatcgcc gcgcggctgc
     4621 ggcgttagaa tcgcattttg tttggagcaa acacgatgaa acagcctgtt tttgccgtta
     4681 cttccggcga gcctgccggc atcggccccg atatttgttt ggacttggcg tttgcacgcc
     4741 tgccctgccg ctgcgcggta ttgggcgaca aaaatctatt gcgcgcgcgc gccgaagcct
     4801 tgggcaaaag cgtcgtcttg cgcgacttcg atccggaatc aggcggcgcg gcatacggcg
     4861 agctggaagt gctgcacatc cctgccgccg aagcggttga ggcagggcgg ctcaatcccg
     4921 ccaacgccgc ctatgtgctg caacttttgg ataccgcgct cgcaggcatt tcagacggca
     4981 ttttcgacgg catcgttacc gcgccgctgc acaaaggcat catcaacgac gcgcgcgcaa
     5041 gcacaggttt tttcagcgga cacaccgaat atctggcgga aaaaagcggc acggggcagg
     5101 tcgtgatgat gcttgccggc aaaggcctgc gcgtcgccct cgtaacgacc cacctgccgc
     5161 tgaaagacgt tgccgccgcc atcacgcaac cgctgattga aagcgtcgca cgcattctgc
     5221 atcacgactt aaaacacaaa ttcggcatca aaaatcccaa aatcctggtc gccggactca
     5281 acccccacgc cggcgaaggc ggacacctcg gacacgaaga aaccgacacc attatccccg
     5341 cgctcgaaaa cctgcgccgc gaagggataa acctcgccgg cccgtatccg gcggatacct
     5401 tgttccaacc gtttatgctc gaaggtgcgg atgccgtatt ggcgatgtac cacgaccaag
     5461 ggctgcccgt gttgaaatac cacagcttcg gacagggcgt gaacatcacg ctgggtctgc
     5521 cctttatccg cacctccgtc gatcacggca ccgcgctcga tttggcggca accggcaggg
     5581 cggattccgg cagcctgata actgccgtgg agaccgccgt cgagatggcg cgcggcagcc
     5641 tttaaagatg ataaaagacc cgtcatttcc gcgcaggcgg catcgtccac cctccgccgt
     5701 cattcccgcg caggcgggaa tccagcctcg tcggtacgga aacttatcgg aaaaaacagt
     5761 ttctccaacc ctgcgttcta gattcccgct tttgcgggaa tgacgaattt aggtttctgt
     5821 ttttggtttt ctgtccttgt gggaatgatg aaattttaag ttttaggaat ttatcggaaa
     5881 aaacagaaac cgctccgccg tcattcccgc gcaggcggga atccagacca ttggacagcg
     5941 gcaatattca aagattatcc gaaagtttga gattctagat tcccgttttc acgggaatga
     6001 cgaaaggttg tgggaatcca gaccttcggg cagcggcaat attcaaagat tatctgaaag
     6061 tttagaggtt ctagattccc gttttcacgg gaatgacgga a
//
LOCUS       DAWFHY010000102         5964 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.102, whole genome shotgun sequence.
ACCESSION   DAWFHY010000102 DAWFHY010000000
VERSION     DAWFHY010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5964
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.102"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..235)
                     /locus_tag="WGQ68_001843"
     CDS             complement(<1..235)
                     /locus_tag="WGQ68_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0715604.1"
                     /translation="MKELDKIDFRILKILQKNARIPMTELAEKVGLSTTPVTERVRRL
                     EREHYISGYHAHLNPHLLGKPLLVFVELKLQSKS"
     gene            460..1518
                     /gene="alr"
                     /locus_tag="WGQ68_001844"
     CDS             460..1518
                     /gene="alr"
                     /locus_tag="WGQ68_001844"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEZ0715605.1"
                     /translation="MRPLNVQIRLGNLRHNYQILKEMHGGKLLAVVKADAYGHGAVRC
                     AFALADLADGFAVATIDEGIRLRESGITHPIVLLEGVFEASEYEAVEQYSLWPAVGNQ
                     WQLEALLSRHWKKPVKVWLKMDSGMHRTGFFPHDYASAYAALKQSEYVDSIVKFSHFS
                     CADEPESGMTEIQMEAFDLGTEGLEGEESLANSAAILNVPEARRDWGRAGLALYGISP
                     FGGGDDRLKPVMRLSTRIFGERVLQPHSPIGYGATFYTSKSTRVGLIACGYADGYPRR
                     APSNSPVAVDGKLTRVIGRVSMDMMTIELDASQEGLGHEVELWGDTVNINTVAEAAGT
                     IPYELMCNIKRAKFTYIE"
     gene            complement(1582..2937)
                     /locus_tag="WGQ68_001845"
     CDS             complement(1582..2937)
                     /locus_tag="WGQ68_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEZ0715606.1"
                     /translation="MSIQSGEILETVKMVADQNFDVRTITIGIDLHDCISTDIDVLNQ
                     NIYNKITTVGKDLVATAKYLSAKYGVPIVNQRISVTPIAQIAAATHADSYVSVAQTLD
                     KAAKAIGVSFIGGFSALVQKGMSPSDEVLIRSIPEAMKTTDIVCSSINVGSTRAGINM
                     DAVKLAGETIKRTAEITPEGFGCAKIVVFCNAVEDNPFMAGAFHGSGEADAVINVGVS
                     GPGVVKAALENSDATTLTEVAEVVKKTAFKITRVGELIGREASKMLNIPFGILDLSLA
                     PTPAVGDSVARILEEMGLSVCGTHGTTAALALLNDAVKKGGMMASSAVGGLSGAFIPV
                     SEDEGMIAAAEAGVLTLDKLEAMTAVCSVGLDMIAVPGDTPAHTISGIIADEAAIGMI
                     NSKTTAVRIIPVTGKTVGDSVEFGGLLGYAPVMPVKEGSCEVFVKRGGRIPAPVQSMK
                     N"
     gene            complement(2952..3224)
                     /locus_tag="WGQ68_001846"
     CDS             complement(2952..3224)
                     /locus_tag="WGQ68_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEZ0715607.1"
                     /translation="MNNSVITVIGKDRVGIVYDVSKILAENQINILNISQQLMDDFFT
                     MIILVDTSKCSKSRQEVLDLFAEESKKLALDIRMQNEEIFQAMHRI"
     gene            complement(3350..4087)
                     /locus_tag="WGQ68_001847"
     CDS             complement(3350..4087)
                     /locus_tag="WGQ68_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HEZ0715608.1"
                     /translation="MKKLSRIVFSIVLLGFSASLSAQTYSVYFNQNGKLTATMSSAAY
                     IRQYSVAEGIAYAQDFYYPSMKKYSEPYIVASTQIKSFVPTLQNGMLILWHFNGQKKM
                     AGGFSKGKPDGEWVNWYPNGKKSAVMPYKNGLSEGTGYRYYRNGSKESEIQFKQNKAN
                     GVWKQWYADGNIKTEMVMVNDEPAKILTWDESGRLLSELSIHHHKRNGVVLEWYEDGS
                     KKSEAVYQDDKLVRKTQWDKDGYLIEP"
     gene            complement(4368..5267)
                     /gene="prmB"
                     /locus_tag="WGQ68_001848"
     CDS             complement(4368..5267)
                     /gene="prmB"
                     /locus_tag="WGQ68_001848"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014580280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEZ0715609.1"
                     /translation="MFNQAAQELTTIRDVLRFAVSRFNEAGLFFGHGTDNAHDEAAYL
                     ILHTLNLPLDMLAPYLDAKLLEAEKEEVLTVIERRAVEHIPAAYLTHQAWQGEFDFYV
                     DERVIIPRSFIYELLGNGLRPWIEYDELVHNALDLCTGSGCLAIQMAHHYPDAQIDAV
                     DVSLDALEVAGINVEDYGLEERIRLIHTDLFEGLEGTYDLIVSNPPYVDAESVELLPE
                     EYLHEPELALGSGADGLDATRQILLNAAKFLNPKGVLLVEIGHNRDVLEAAYPELPFT
                     WLETSGGDGFVFLLTREQLLGEE"
     gene            5412..>5964
                     /locus_tag="WGQ68_001849"
     CDS             5412..>5964
                     /locus_tag="WGQ68_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715610.1"
                     /translation="MADGMGNLIGWEKTGLVAGKQWITAKDDKVSDVCNANGEMGVIG
                     LYEPFSHGALTIPGHPNCRCEVVSVSGGELGEFAEKKELRKAAMQYARDNFIGKSYVN
                     ENSGHELKVTWQGVKHAASKANQAELSIITKLDDLLRYAKYEGSYSDRKGRPDIIAAH
                     KYRAVAKVGNESLNIGVIVREFPD"
BASE COUNT         1618 a         1498 c         1344 g         1504 t
ORIGIN      
        1 ccgatttgga ttgcagcttc agctcgacaa aaaccaataa aggtttgccc agcaggtggg
       61 gattgagatg ggcgtgataa ccggaaatat aatgttcccg ctccaaacgg cgtacccgtt
      121 cggtaaccgg ggtagtggac aatcctactt tctcggcaag ctccgtcatg ggaatacggg
      181 catttttctg caggatcttg aggatacgga aatcaatctt atctaactct ttcatttgtc
      241 tttaccttat tgtttattat tgattttaat ggtttggatg ctgataagga aagacaatta
      301 tatcaggaaa acacaagcct cctttcccta tggcaaaccc aacctcacct ccggagagta
      361 aggttcttta atattttaaa agccttgccg ttggattata attccctaat tgatttctta
      421 attttgctaa taaacacttg tttggtaagg aatgaattta tgcgcccttt gaacgtgcag
      481 atcaggttgg gcaaccttag gcacaattat cagattttaa aggaaatgca cggaggcaaa
      541 ctgttggcgg tagtgaaggc cgacgcatac ggacacggtg cggtcagatg tgctttcgcg
      601 ctggcagact tggcagacgg ttttgccgtg gcgacaatcg atgaaggaat caggctgcgg
      661 gagagcggca ttacccatcc gattgtcctt ttggaaggcg tatttgaagc atcagagtac
      721 gaagcggtcg aacaatactc gctctggccg gcagtcggaa accaatggca acttgaggct
      781 ttgctttccc gccattggaa aaaacctgtc aaggtctggt tgaaaatgga ttcgggaatg
      841 caccgtaccg gttttttccc tcatgattac gcttcggcat atgcggcatt gaagcagtcg
      901 gaatatgtgg acagtattgt caagttttcg catttttcct gtgcggacga acccgaaagc
      961 ggtatgacgg aaatacagat ggaagcattc gatttgggta cggaagggct ggaaggcgaa
     1021 gaaagccttg ccaactccgc cgctattttg aatgttcccg aagcacgcag ggactggggg
     1081 cgcgccggtc tggcgttata cggcatttcc ccgttcggag gaggcgatga caggctgaag
     1141 cccgtgatga ggctttccac ccgtattttc ggcgaacgcg ttttacagcc gcactcccct
     1201 atcggttatg gcgcaacatt ttataccagc aaatctacgc gcgtcggtct gattgcctgc
     1261 ggttatgcgg acggttatcc gcgccgcgcc ccaagcaatt cccccgtcgc cgtcgacggc
     1321 aaattgaccc gggtcatcgg cagggtctct atggatatga tgaccatcga gctggacgct
     1381 tcgcaagaag gtttgggaca cgaggtcgaa ctgtggggcg atacggtcaa catcaatact
     1441 gttgccgaag cggccggaac catcccttac gaattgatgt gcaatatcaa acgtgcaaaa
     1501 ttcacttata tcgagtaatc aagtccaaac aaaaatgccg tctgaagcct ttcagacggc
     1561 atttccccat caaaaccgca atcagttttt catcgattgg accggagcgg gaattctgcc
     1621 gccccgtttg acgaacactt cgcacgaacc ttcttttacc ggcatcacag gtgcgtagcc
     1681 caacaggccg ccaaactcga cgctgtcgcc gacggtttta ccggttaccg gaataatgcg
     1741 cacggcagtg gttttgctgt tgatcatgcc gatggcggct tcgtcggcga tgatgccgga
     1801 aatggtatga gcaggcgtat cgccgggaac ggcaatcata tccaaaccaa cggagcagac
     1861 agcggtcatc gcttcaagtt tgtccaacgt cagcacaccg gcttcggcag cggcaatcat
     1921 gccctcgtct tctgaaacgg ggataaacgc accgctcaaa ccgccgaccg cgctggaagc
     1981 catcatgccg ccctttttca cggcatcgtt cagcaatgcc aaagccgccg tcgtgccgtg
     2041 tgtaccgcag acgctcaaac ccatttcttc aagaatgcgc gccaccgagt cgccgacggc
     2101 aggggtcggt gccaacgaca agtcgagaat accaaacggg atattcagca tttttgaggc
     2161 ttcgcggccg atgagttcac ccacgcgggt aattttgaaa gcagttttct tcactacttc
     2221 cgcaacttcg gtcaatgtcg ttgcatctga attttccaac gcggctttga ccacgcccgg
     2281 ccccgatacg ccgacattaa tcacagcatc cgcttcgcct gagccgtgaa acgcgcccgc
     2341 cataaacgga ttgtcttcca ccgcattgca gaacacgacg attttcgcgc agccgaaacc
     2401 ttcgggcgta atttcagccg tgcgtttgat ggtttcgcct gccagcttga ccgcatccat
     2461 attgataccg gcgcgcgtac tgccgacatt gatggagctg cacacgatgt cggtggtctt
     2521 catcgcttcg ggaatggaac ggattaacac ctcgtctgaa ggcgacatgc ctttttgcac
     2581 caacgcggaa aagccgccga taaaggacac gccgatggct ttggcagcct tatccaaagt
     2641 ttgcgccacg ctgacatagg aatcggcatg ggtagccgcc gcgatttggg caatcggcgt
     2701 aacggaaatg cgctgattca caatcggcac gccgtatttg gcagacagat attttgccgt
     2761 cgccaccaag tctttgccga ccgtggtgat tttgttgtaa atgttttggt ttaacacgtc
     2821 gatgtcggtg ctgatgcagt cgtgcaaatc aatgccgatg gtaatggtgc ggacatcaaa
     2881 attctggtcg gcaaccattt tgacggtttc taaaatttcg ccggattgga tactcatcac
     2941 attcctccaa ctcaaatgcg gtgcatcgct tggaagattt cttcgttttg catacggata
     3001 tcaagcgcga gttttttgct ctcttccgca aacaaatcca aaacctcttg acgcgatttg
     3061 ctgcattttg aagtgtccac caagataatc atagtaaaaa aatcgtccat cagctgttgg
     3121 ctgatgttga gaatattgat ttggttttcc gccaaaattt tggaaacatc gtacacgatg
     3181 ccgacgcggt ctttaccgat gacggtgatg actgaattgt tcacaggctt actccttgca
     3241 gatatccgtt aaagtccgaa attataccac cgttggattt tgaagaaata ttgtcaacaa
     3301 tatatacata caaaatgccg tctgaaacta tttcagacag catcaagatt cagggttcga
     3361 ttaaataacc atccttatcc cactgggttt tcctgaccaa cttgtcatcc tgataaacag
     3421 cttcactctt tttagaacca tcttcatacc actctaagac tactccatta cgtttatgat
     3481 gatggataga cagttccgag agtaatcgac cgctttcatc ccatgtcaga atcttggccg
     3541 gctcatcatt gaccataacc atttccgttt tgatattgcc atcagcatac cattgtttcc
     3601 atacgccgtt tgccttattt tgtttaaact ggatttcgct ttccttgctg ccattacggt
     3661 aatagcggta ccccgtacct tcactcaaac catttttata aggcataacc gcagattttt
     3721 taccgttcgg ataccagttg acccactcac cgtccggctt acccttgctg aagccccccg
     3781 ccattttttt ctgaccatta aaatgccaca aaatcaacat accgttttgc agggtaggca
     3841 caaaagattt gatttgcgtt gaagcaacga tataaggttc ggaatatttc ttcatcgacg
     3901 gataataaaa atcctgcgcg tacgcaatac cctccgccac actatattgc ctgatataag
     3961 cggcagaaga catcgtcgcc gtcagtttcc cgttctgatt aaaataaaca gaataggtct
     4021 gtgccgataa agaagccgaa aaacccaaca ggacaattga aaatacaatc cgagataatt
     4081 ttttcattgc aatagcgata taaaaacaag gctgtgtttt agtaatctgt tgatttcaat
     4141 tatttgcaag ggaaaagaca attattttcc ggttaggaat aaacctattc tactgaatat
     4201 attgaagcca agtacgccta tcaacactat attaaaacac tgccaaaaac aattaactta
     4261 taaacaatat ggtaaggatt tctctgccaa gcatcaaacc cgagacaacg tatcgtaaaa
     4321 atgccgtctg aaaacaaatc gtcttcagac ggcatttccc cttcaactca ctcttcaccc
     4381 aataactgct cgcgcgtcaa gaggaaaaca aaaccgtcgc ccccgctggt ttccaaccaa
     4441 gtaaaaggca actccggata cgctgcttcc aatacatccc tgttatgccc gatttccacc
     4501 agcaatacac ctttgggatt cagaaacttt gccgcattca gaagaatctg cctggtggca
     4561 tccaacccgt ccgccccgct gcccaatgcc aattccggtt cgtgcaaata ctcttcaggc
     4621 aataactcaa ccgattccgc atccacataa ggcggattgg aaacaatcaa atcataagtg
     4681 ccttccaatc cttcaaacaa atccgtatga ataagccgga tgcgttcttc caaaccataa
     4741 tcttcgacat taatccctgc cacttccaaa gcatccaagc tcacatcaac cgcatcaatt
     4801 tgggcatcag gataatgatg cgccatctga atggcaaggc aaccgcttcc ggtgcaaaga
     4861 tccaaagcat tatgcaccaa ctcatcgtat tctatccaag gacgaagtcc gttacccaac
     4921 aattcataaa taaaagaacg aggtatgatt acgcgctcat ccacatagaa atcaaactct
     4981 ccctgccatg cctggtgtgt caaataagcg gctggaatgt gttcgacagc acgacgctca
     5041 ataaccgtca gcacttcctc tttttcagct tccaagagtt ttgcatcaag atatggggca
     5101 agcatatcca aaggcaaatt caaagtatgc agaatcaaat aagctgcttc atcatgcgca
     5161 ttatccgttc catgaccaaa aaagagccct gcctcattaa aacggctgac tgcaaaacgt
     5221 aaaacatcgc ggatagtcgt caattcttgt gctgcctgat taaacataat atgaaccatt
     5281 ctgcgtatag atacttttaa ttataacaga aacaacaagc aaaccttttc atatcgccaa
     5341 ataaccaccc aatctaccca tacaactaca taaatgcccg cgcgaaaacc atcgcccgaa
     5401 cggaaacgac aatggccgac ggtatgggca acctgattgg ctgggaaaaa acggggcttg
     5461 ttgccggtaa gcagtggata accgcaaaag acgacaaggt gtccgatgtc tgcaatgcca
     5521 acggcgagat gggcgtaatc gggctttacg agcctttctc acacggcgca ttgacgatac
     5581 ccggtcatcc gaactgccga tgcgaggttg tttccgtatc gggtggcgaa ttgggggaat
     5641 ttgccgaaaa aaaggagctt cgtaaagcgg ctatgcagta tgcgcgggat aactttatcg
     5701 gcaaaagcta tgtcaatgaa aacagcgggc atgaactgaa ggtaacttgg caaggtgtga
     5761 aacacgctgc gtcaaaggca aatcaggcgg aattatccat cataacaaaa cttgatgact
     5821 tattgcgcta cgcaaaatat gagggttctt attcggatag gaaaggtcgt cctgatatta
     5881 ttgcagcaca taagtatcgt gccgttgcca aggttgggaa tgagtcttta aatatcggcg
     5941 tgattgtaag ggaatttcca gacg
//
LOCUS       DAWFHY010000103         5710 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.103, whole genome shotgun sequence.
ACCESSION   DAWFHY010000103 DAWFHY010000000
VERSION     DAWFHY010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5710
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.103"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(284..397)
                     /gene="rrf"
                     /locus_tag="WGQ68_001850"
     rRNA            complement(284..397)
                     /gene="rrf"
                     /locus_tag="WGQ68_001850"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(494..3385)
                     /locus_tag="WGQ68_001851"
     rRNA            complement(494..3385)
                     /locus_tag="WGQ68_001851"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(3792..3867)
                     /locus_tag="WGQ68_001852"
     tRNA            complement(3792..3867)
                     /locus_tag="WGQ68_001852"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3832..3834),aa:Ala,seq:tgc)
     gene            complement(3873..3949)
                     /locus_tag="WGQ68_001853"
     tRNA            complement(3873..3949)
                     /locus_tag="WGQ68_001853"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3913..3915),aa:Ile,seq:gat)
     gene            complement(4051..5591)
                     /locus_tag="WGQ68_001854"
     rRNA            complement(4051..5591)
                     /locus_tag="WGQ68_001854"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1243 a         1663 c         1226 g         1578 t
ORIGIN      
        1 tgcgaagccg cattgatgtt taccgccgca agggagaagg cgcaggagaa cagcatacag
       61 aacagcacga acattttctt catggttttt cctttaaggg ttgcaaacaa taaaccgcat
      121 cttgcgacga taaaacgagt cattctaaaa tgaatatccc aaagtttcaa gccgttcctc
      181 cgcaaacccg accggacgcc gtacggatac tgtcccgcca tccccgacat tttttccggg
      241 caaagcaaaa acccccgaat aatcgggggt tttctgaatg ggtgtttggc agtgacctac
      301 tttcgcatgg aagaaccaca ctatcatcgg cgctgagtcg tttcacggtc ctgttcggga
      361 tgggaaggcg tgggaccaac tcgctatggc cgccaaactt aaactgttac aaatcggtaa
      421 agccttaatc aatatattcg gtgatgactg aatcagtcag taagctttta tctcttaaag
      481 ttcttcaaat gatagagtca agcctcacga gcaattagta tgggttagct tcacgcgtta
      541 ccgcgcttcc acaccccacc tatcaacgtc ctggtctcga acgactcttt agtgcggtta
      601 aaccgcaagg gaagtctcat cttcaggcga gtttcgcgct tagatgcttt cagcgcttat
      661 ctcttccgaa cttagctacc cggctatgca actggcgtta caaccggtac accagaggtt
      721 cgtccactcc ggtcctctcg tactaggagc agcccccgtc aaacttccaa cgcccactgc
      781 agatagggac caaactgtct cacgacgttt taaacccagc tcacgtacca ctttaaatgg
      841 cgaacagcca tacccttggg accgactaca gccccaggat gtgatgagcc gacatcgagg
      901 tgccaaactc cgccgtcgat atgaactctt gggcggaatc agcctgttat ccccggagta
      961 ccttttatcc gttgagcgat ggcccttcca tacagaacca ccggatcact atgtcctgct
     1021 ttcgcacctg ctcgacttgt cggtctcgca gttaagctac cttttgccat tgcactatca
     1081 gtccgatttc cgaccggacc taggtaacct tcgaactcct ccgttacgct ttgggaggag
     1141 accgccccag tcaaactgcc taccatgcac ggtccccgac ccggatgacg ggtctgggtt
     1201 agaacctcaa agacaccagg gtggtatttc aaggacggct ccacagagac tggcgtctct
     1261 gcttctaagc ctcccaccta tcctacacaa gtgacttcaa agtccaatgc aaagctacag
     1321 taaaggttca cggggtcttt ccgtctagca gcgggtagat tgcatcttca caaccacttc
     1381 aacttcgctg agtctcagga ggagacagtg tggccatcgt tacgccattc gtgcgggtcg
     1441 gaacttaccc gacaaggaat ttcgctacct taggaccgtt atagttacgg ccgccgttta
     1501 ctggggcttc gatccgatgc tctcacatct tcaattaacc ttccagcacc gggcaggcgt
     1561 cacaccctat acgtccactt tcgtgttagc agagtgctgt gtttttaata aacagtcgca
     1621 gccacctatt ctctgcgacc ctccggggct tacggagcaa gtccttaacc ttagagggca
     1681 taccttctcc cgaagttacg gtatcaattt gccgagttcc ttctcctgag ttctctcaag
     1741 cgccttagaa ttctcatcct gcccacctgt gtcggtttgc ggtacggttc gattcaaact
     1801 gaagcttagt ggcttttcct ggaagcgtgg tatcggttgc ttcgtgtccg tagacactcg
     1861 tcgtcacttc tcggtgttaa gaagacccgg atttgcctaa gtcttccacc taccggctta
     1921 aacaagctat tccaacagct tgccaaccta accttctccg tccccacatc gcatttgaat
     1981 caagtacagg aatattaacc tgtttcccat cgactacgca tttctgcctc gccttagggg
     2041 ccgactcacc ctacgccgat gaacgttgcg taggaaacct tgggctttcg gcgagcgggc
     2101 ttttcacccg ctttatcgct actcatgtca acattcgcac ttctgatacc tccagcacac
     2161 tttacaatgc accttcatcg gcctacagaa cgctccccta ccatgccggt aaaccggcat
     2221 ccgcagcttc ggttatagat ttgagccccg ttacatcttc cgcgcaggac gactcgacca
     2281 gtgagctatt acgctttctt taaatgatgg ctgcttctaa gccaacatcc tggctgtctg
     2341 ggccttccca cttcgtttac cacttaatct atcatttggg accttagctg gcggtctggg
     2401 ttgtttccct cttgacaacg gacgttagca cccgctgtct gtctcccgag gaaccacttg
     2461 atggtattct tagtttgcca tgggttggta agttgcaata accccctagc cataacagtg
     2521 ctttaccccc atcagtgtct tgctcgaggc actacctaaa tagttttcgg ggagaaccag
     2581 ctatctccga gtttgtttag cctttcaccc ctatccacag ctcatccccg cattttgcaa
     2641 catgcgtggg ttcggtcctc cagtacctgt tacggcacct tcaacctggc catggataga
     2701 tcactcggtt tcgggtctac acccagcaac tcatcgccct attaagactc ggtttcccta
     2761 cgcctcccct attcggttaa gctcgctact gaatgtaagt cgttgaccca ttatacaaaa
     2821 ggtacgcagt cacacctcta gggtgctccc actgtttgta tgcatcaggt ttcaggttct
     2881 atttcactcc cctcccgggg ttcttttcgc ctttccctca cggtactggt tcactatcgg
     2941 tcgatgatga gtatttagcc ttggaggatg gtccccccat attcagacag gatttcacgt
     3001 gtcccgccct acttttcgta cgcttagtac cgctgttgag atttcgaata cgggactgtc
     3061 acccactatg gtcaagcttc ccagcttgtt cttctatctc gacagttatt acgtacaggc
     3121 tcctccgcgt tcgctcgcca ctacttgcgg aatctcggtt gatttctttt cctccgggta
     3181 cttagatggt tcagttctcc gggttcgctt ctctaagtct atgtattcaa cttaggatac
     3241 tgcacagaat gcagtgggtt tccccattcg gacatcgcgg gatcattgct ttattgccag
     3301 ctcccccgcg cttttcgcag gcttacacgt ccttcgtcgc ctatcatcgc caaggcatcc
     3361 gcctgatgca cttattcact tgactctatc atttcaagaa cttctttgac tttgcctaac
     3421 attccgttga ctagaacatc agacttgaat ttcctacttt gataaagctt actgctttgt
     3481 tgtgtcttaa tcctgccttt tgtgtttcag gattaagtcg atacaatcat cacccaaata
     3541 ctgtgtttgt tttcttttct cttgtgagcg attttttatc ctttgcaaag aataaaaaat
     3601 caaaacaaac gctttgtctt tgtttgttga tttcggcttt ccaatttgtt aaagatcgat
     3661 gcgttcgata ttgctatcta ctgtgcaaat caaaacgagc tgattattat atcagcattt
     3721 tgttcttggt caagtgtgac gtcgccctga atggattctg ttccattctt ccgttttgat
     3781 ttgtacagta ttggtggagg caaacgggat cgaaccgatg accccctgct tgcaaagcag
     3841 gtgctctacc aactgagcta tgcccccgtt cttggtgggt ctgggaggac ttgaacctcc
     3901 gaccccacgc ttatcaagcg tgtgctctaa ccagctgagc tacaaacccg gattctcttc
     3961 ttaagcgaat cttgccttca ctcaagcttc ttccgcatct ttttcagttt accgataagt
     4021 gtgaatgcct aaagcctctt ctttctctag aaaggaggtg atccagccgc aggttcccct
     4081 acggctacct tgttacgact tcaccccagt catgaagcat accgtggtaa gcggactcct
     4141 tgcggttatc ctacctactt ctggtatccc ccactcccat ggtgtgacgg gcggtgtgta
     4201 caagacccgg gaacgtattc accgcagtat gctgacctgc gattactagc gattccgact
     4261 tcatgcactc gagttgcaga gtgcaatccg gactacgatc ggttttgtga gattggctcc
     4321 gcctcgcggc ttggctaccc tctgtaccga ccattgtatg acgtgtgaag ccctggtcat
     4381 aagggccatg aggacttgac gtcatcccca ccttcctccg gcttgtcacc ggcagtctca
     4441 ttagagtgcc caactgaatg atggcaacta atgacaaggg ttgcgctcgt tgcgggactt
     4501 aacccaacat ctcacgacac gagctgacga cagccatgca gcacctgtgt tacggctccc
     4561 gaaggcactc ctccgtctcc agaggattcc gtacatgtca agaccaggta aggttcttcg
     4621 cgttgcatcg aattaatcca catcatccac cgcttgtgcg ggtccccgtc aattcctttg
     4681 agttttaatc ttgcgaccgt actccccagg cggtcaattt cacgcgttag ctacgctacc
     4741 aagcaatcag gttgcccaac agctaattga catcgtttag ggcgtggact accagggtat
     4801 ctaatcctgt ttgctaccca cgctttcggg catgaacgtc agtgttgtcc caggaggctg
     4861 ccttcgccat cggtattcct ccacatctct acgcatttca ctgctacacg tggaattcta
     4921 cctccctctg acacactcga gtcacccagt tcagaacgca gttcccgggt tgagcccggg
     4981 gatttcacat cctgcttaag taaccgtctg cgcccgcttt acgcccagta attccgatta
     5041 acgctcgcac cctacgtatt accgcggctg ctggcacgta gttagccggt gcttattctt
     5101 caggtaccgt catcagccgc tgatattagc aacagccttt tcttccctga caaaagtcct
     5161 ttacaacccg aaggccttct tcagacacgc ggcatggctg gatcaggctt gcgcccattg
     5221 tccaaaattc cccactgctg cctcccgtag gagtctgggc cgtgtctcag tcccagtgtg
     5281 gcggatcatc ctctcagacc cgctactgat cgtcgccttg gtaggccttt accccaccaa
     5341 ctagctaatc agatatcggc cgctcgaata gcgcaaggcc cgaaggtccc ctgctttctc
     5401 tctcaagacg tatgcggtat tagctgatct ttcgatcagt tatcccccac tactcggtac
     5461 gttccgatat gttactcacc cgttcgccac tcgccaccca agaagcaagc ttctctgtgc
     5521 tgccgtccga cttgcatgtg taaagcatgc cgccagcgtt caatctgagc caggatcaaa
     5581 ctcttatgtt caatctctaa ctttttaact tctggtctgc ttcaaagaaa ccaacaggac
     5641 aatgttcaaa acattatctt gtctgtcttt caaacagtgt gaggctcaag gcactcacac
     5701 ttatcggtaa
//
LOCUS       DAWFHY010000104         5645 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.104, whole genome shotgun sequence.
ACCESSION   DAWFHY010000104 DAWFHY010000000
VERSION     DAWFHY010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5645
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.104"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>5645
                     /locus_tag="WGQ68_001855"
     CDS             <1..>5645
                     /locus_tag="WGQ68_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ0715611.1"
                     /translation="NVAVNAEGKLVNTGMIAATGENHAVSLHARNVHNSGTVASQDDA
                     NIHSQTLDNSGTVLSSGQLTVRNLGRLKNHNNGTIQAARLDMSTGGLDNTGNITQTGS
                     QALDLVSAGKFDNSGKIGVSDVPQTGLNPNPSVIPQIPSTATGSGSSTVSASKPGSNN
                     PVSPTAPAKNHAGGRIQTTGAFDNAGSINAGGQIDIAAQNGLENSGSLNAAKLRVSGD
                     SFNNTVKGKLQAHDLAVNTQTAKNSGHLLTQTGKIDNRELHNAGEIAANNLTLIHSGR
                     LSNDKKGNIRAAHLQLDTAGLHNAGNILADSGTVTAKNNLRNTGKVSVARLNTEGQTL
                     DNTHGHIEAETVNIQSQQLTNQSGHITATEQLTINSRNVDNQNGKLLSANQAQLAVSD
                     GLNNQHGEIATNRQLSIHDKNQNTLALNNADGTIQSAGNVSLQAKSLANNGTLTAGNK
                     LDIALADDFVVERDLTAGKQLNLSIKGRLKNTHTLQAGHTLKLNAGNIDNQVTGKIIG
                     GEQTDITSEQHVGNRGLINSDGLTHIGAGQTLTNTGTGKIYGNHIALDAQILLNREET
                     TEGSTKAGAIAARKRLDIGAKEIHNQEGALLSSEGIFAVGNRLDEQHHAAGMADTFVN
                     GSAGLEVQGDALMSVRNMQNINNHFKTETYLAKAEKQVRDYTVLGQNTYYQAGKDGLF
                     DNSQGQKDQTTATFHLKNGSRIEANQWHVRDYHIETYKERIIENRPAHITVGGDLTAS
                     GQNWLNKDSRIVVGGRIITDDLNQKEITNQSTTGKGRTDAVGTQWDSVTKKGWYSGRK
                     RQRRTERNHTPYHDTQLFTHDFDTPVSVIQQNAASPSFQPAASAIKLIDGVSTAAVNG
                     QRIHTGNVVSLNNATVTLPNSSLYTTHPDNKGWLVETDPQFADYRRWLGSDYMLQQLQ
                     LDTNHLHKRLGDGYYEQKLVNEQIHQLTGYRRLDGYRSDEEQFKALMDNGLTAAKTFG
                     LTPGIALSAEQVARLTSDIVWMENQTVTLSDGSTQTVLVPKVYALARKGDLNTSGGLI
                     SAEQVLLKLQNGNLTNSGTIAGRQAVLIQARNINSNGNIQADQIGLKAEKSINIDGGQ
                     VQAGRLLTAQAQNINLNGTTQTSGNERNGNTAIDRMAGINVVGSHTEQVDNRTSDGIL
                     SLHASNDINLNAATVSNQVKDGTTQITAGNNLNLGTIRTEHREAYGTLDDENHRHVRQ
                     STEVGSSIRTQNGALLRAGNDLKIRQGELEAEEGKTVLAAGRDVTISEGRQITELDTS
                     VSGKSKGILSSTKTQDRYRFSHDEAVGSNIGGGKMIVAAGQDINVRGSNLISDKGIVL
                     KAGHDIDISTAHNRYTGNEYHESKKSGVMGTGGLGFTIGNLKTTDDTDRTNIVHIGSI
                     IGSLNGDTVTVAGNRYRQTGSTVSSPEGRNTVTAKSIDVESANNQYATDYAHTQEQKG
                     LTVALNVPVVQSAQNFIQAAQNVGKSKNKRVNAMAAANAAWQGYQAAQQMQQFAPSSS
                     AGQGQNNNQSPSISVSITYGEQKSRNEQKRHYTEAAASQIIGKGQTTLAATGSGEQSN
                     INITGSDVIGHAGTALIADNHIRLQSAKQDGSEQSKNKSSGWNAGVAVKIGNGISFGI
                     TAGGNIGKGKEQGGSTTHRHTHVGSTTGKTTIRSGGDTTLKGVQLIGKGIQADTRNLH
                     IESVQDTETYQSKQQNGNVQVTVGYGFSASGSYRQSKVKADHASVTGQSGIYAGEDGY
                     QIKVRDNTDLKGGIITSSQSAEDKGKNLFQTATLTASDIQNHSRYEGRSFGIGGSFDL
                     NGGWDGTVTDKQGRPTDRISPAAGYGSDGDSKNSTTRSGVNTRNIHITDEAGQLARTG
                     RTAKETEARIYTGIDTETADQHSGRLKNSFDKDAVAKEI"
BASE COUNT         1816 a         1474 c         1286 g         1069 t
ORIGIN      
        1 aatgtggcag tgaatgctga gggtaaactg gtcaacacag gcatgattgc agcgacggga
       61 gaaaatcatg cggtttcact tcatgcccgc aatgttcata atagcggtac ggttgcctca
      121 caggatgatg ccaatattca cagccagacg ctggacaatt caggtacggt cttatcctca
      181 ggtcaattaa ctgtccgtaa tttaggccgt ctgaaaaacc ataacaacgg tacgatccag
      241 gctgcacgct tagatatgtc aacaggtggt ttggataaca caggtaatat tactcaaaca
      301 ggttcacaag cattggattt ggtatctgcc ggcaaattcg ataacagtgg caagattggt
      361 gtgagcgacg ttccacagac cggtttgaat cccaatccat cagtcatacc acagattccg
      421 agtactgcaa caggttcagg cagcagcact gtctcggcat ctaagcctgg ttcaaacaat
      481 cccgtttcac ctacagcacc tgcaaaaaac cacgccggag gacgcattca aacaacagga
      541 gcatttgaca atgcaggatc aatcaatgca ggtgggcaaa ttgacattgc cgcccaaaac
      601 ggtttggaaa attcgggtag tctgaatgcg gctaaactac gagtatcagg cgattcattt
      661 aacaatacgg taaaaggcaa actccaggca cacgatctgg ctgttaacac tcaaactgct
      721 aaaaacagcg gtcacttatt aactcaaacc ggcaagattg acaaccgtga actgcataat
      781 gccggagaaa ttgccgccaa caatctgaca ctcattcatt cgggccgctt gagcaatgat
      841 aaaaaaggca atattcgagc tgcacattta cagcttgata ccgccggttt acataatgca
      901 ggtaacattc ttgccgatag tggaaccgtt accgccaaga ataatcttcg caatacagga
      961 aaagtttctg ttgcacgact gaataccgaa ggtcagactc tagataatac gcacggacat
     1021 atagaggctg aaacggttaa catccaaagt cagcaactga ctaaccaaag cggccatatt
     1081 actgctaccg aacaactgac tatcaatagt cgaaatgtag acaaccaaaa cggcaaactc
     1141 ctatctgcaa accaagcaca attagctgtt tcagacggcc taaacaacca acatggtgaa
     1201 attgccacca accggcagtt gtctattcac gataaaaatc aaaacacttt ggcgttaaac
     1261 aatgcggatg gcacgattca atctgccggt aatgtatcgc tacaagccaa atcactcgcc
     1321 aacaatggca cattaacagc cggtaacaaa ctggatattg ctttggcgga cgatttcgtc
     1381 gtagagcgcg acctcactgc aggcaaacaa ttaaatctaa gcataaaagg ccgtctgaaa
     1441 aatacccata ccctacaagc aggccatacg ctcaaactca atgccggcaa tatagataac
     1501 caagttacag gcaaaattat tggcggagaa caaacggaca tcacatccga acagcatgtt
     1561 ggcaacaggg gcttgatcaa cagcgacggt ttgacccaca tcggtgcagg tcaaaccctg
     1621 accaacaccg ggacaggcaa aatctatggc aaccatattg ccctggacgc gcaaatactg
     1681 cttaaccggg aagaaacgac ggaaggcagt accaaagcgg gggcaatagc tgcaaggaaa
     1741 cgtttggata ttggagcgaa agagattcat aaccaagaag gtgccctact atccagcgaa
     1801 ggtatttttg ccgtaggtaa tcgactggat gaacaacatc atgcggcagg catggccgat
     1861 acctttgtta atggcagtgc cggtttggaa gtacaaggtg atgcattgat gtccgttcgg
     1921 aatatgcaga atatcaataa tcactttaaa acagagacat acttagccaa agcggaaaag
     1981 caagtccgcg actacaccgt actggggcaa aatacctact atcaggcggg aaaagacggt
     2041 ttattcgaca actcgcaagg acaaaaagac caaactactg ctacgttcca tttaaaaaat
     2101 ggttctcgta ttgaggccaa ccaatggcat gtccgagact accacatcga gacttataaa
     2161 gaacgcatca tcgaaaaccg gccggcacac attactgtgg gcggtgattt gactgcctca
     2221 ggtcaaaatt ggctgaacaa agacagccgg attgtagtag gcgggcgtat tatcactgat
     2281 gatttaaacc agaaagaaat taccaatcaa agtacaacag gcaaaggtcg cacagatgct
     2341 gtcggcacac agtgggattc agttacaaaa aaaggatggt acagcggtag aaaaagacaa
     2401 cgccgtactg aaagaaacca tactccttac catgataccc aactatttac ccacgacttc
     2461 gacacgcctg tatccgtcat ccaacagaat gccgcctccc cttcctttca acccgccgca
     2521 tctgcaatca aactgattga cggagtatcc acggcagccg tcaatggtca gcgcatccat
     2581 accggtaatg tggtctcgtt aaataacgct actgttactc tgcctaacag cagcctctat
     2641 accacccatc ctgacaataa aggctggttg gttgaaaccg atcctcaatt tgcagactac
     2701 cgccgctggt tgggcagcga ctacatgttg caacaactgc aattggacac caatcatcta
     2761 cacaaacggc ttggcgacgg ctactacgaa caaaaacttg ttaatgaaca aatccatcag
     2821 ttaacaggct accgccgact cgacggctac aggagtgatg aagaacaatt caaagctctg
     2881 atggacaacg gccttactgc tgccaaaaca ttcggtctca ccccaggtat cgccttgagt
     2941 gcagagcaag ttgcccgctt aacttcagat atcgtttgga tggaaaatca aaccgtcacc
     3001 ctgtctgacg gttcgactca aaccgtactg gttcctaaag tctatgccct ggcgcgcaaa
     3061 ggtgatctca atacctccgg tggcctgatt agtgccgaac aagtcttact taaactgcaa
     3121 aacggcaacc tgactaacag cggtaccatt gcggggcgac aggccgtact catccaggca
     3181 cggaatatta acagcaacgg taacattcaa gccgaccaaa tcggcttaaa agctgaaaaa
     3241 agtatcaata tcgacggcgg gcaggtacaa gcaggcagac tgctgactgc ccaagcgcaa
     3301 aatatcaacc ttaacggtac aacccaaact tccggtaatg aacgtaacgg caataccgcc
     3361 atcgatcgta tggccggcat taacgtggtc ggaagccata ctgaacaagt agataacaga
     3421 acttcagacg gcatcctatc cctgcatgcc agcaacgata tcaacctcaa tgcggccacc
     3481 gtctctaacc aagttaaaga cggcactacc caaattaccg ccggcaataa tctcaacctc
     3541 ggcaccatcc gtaccgaaca tcgcgaagcc tatggtacat tagatgacga gaaccatcgc
     3601 catgtccgcc aaagtaccga agtcggcagc agtatccgca cgcaaaacgg cgcactgctt
     3661 agagccggta acgacttaaa aatccgccaa ggcgaactgg aggccgaaga aggcaaaacc
     3721 gtccttgccg caggacgtga tgtcactatc agcgaaggac gccaaataac cgaactggat
     3781 acctcggtaa gcggaaaaag caaaggcatc ctttccagta ccaaaacaca ggatcgctac
     3841 cgcttcagtc atgatgaagc agtcggcagc aacatcggcg gcggcaaaat gattgttgca
     3901 gccgggcagg atatcaatgt acgcggcagc aaccttattt ctgataaggg cattgtctta
     3961 aaagcaggac acgacatcga tatttctact gcccataatc gctataccgg caatgaatac
     4021 cacgagagca aaaaatcagg cgtcatgggt actggcggat tgggctttac tatcggtaac
     4081 ctgaaaacta ccgatgacac tgatcgtacc aatattgtcc atataggcag cattataggc
     4141 agcctgaatg gagacaccgt tacagttgca ggaaaccgct accgacaaac cggcagtacc
     4201 gtctccagcc ccgaggggcg caataccgtc acagccaaaa gcatagatgt agagtccgca
     4261 aacaaccagt atgccactga ctacgcccat acccaggaac aaaaaggcct taccgtcgcc
     4321 ctcaatgtcc cggttgtcca atctgcacaa aacttcatac aagcagccca aaatgtgggc
     4381 aaaagtaaaa ataaacgcgt taatgccatg gctgcagcca atgctgcatg gcagggttat
     4441 caagcagccc aacaaatgca acaatttgct ccaagcagca gtgcgggaca aggtcaaaac
     4501 aacaatcaaa gccccagtat cagtgtgtcc atcacctacg gcgaacagaa aagtcgtaac
     4561 gagcaaaaaa gacattacac cgaagcggca gcaagtcaaa ttatcggcaa agggcaaacc
     4621 acacttgcgg caacaggaag tggggagcag tccaatatca atattacagg ttccgatgtc
     4681 atcggccatg caggtactgc cctcattgcc gacaaccata tcagactcca atctgccaaa
     4741 caggacggca gcgagcaaag caaaaacaaa agcagtggtt ggaatgcagg cgtagccgtc
     4801 aaaataggca acggcatcag ttttggaatt accgccggag gaaatatcgg taaaggtaaa
     4861 gagcaagggg gaagtactac ccaccgccac acccatgtcg gcagcacaac cggcaaaact
     4921 accatccgaa gcggcgggga taccaccctc aaaggtgtgc agctcatcgg caaaggcata
     4981 caggcagata cgcgcaacct gcatatagaa agtgttcaag atactgaaac ctatcagagc
     5041 aaacagcaaa acggcaatgt ccaagttact gtcggttacg gattcagtgc aagcggcagt
     5101 taccgccaaa gcaaagtcaa agcagaccat gcctccgtaa ccgggcaaag cggtatttat
     5161 gccggagaag acggctatca aatcaaagtc agagacaaca cagacctcaa gggcggtatc
     5221 atcacgtcta gccaaagcgc agaagataag ggcaaaaacc tttttcagac ggccaccctt
     5281 actgccagcg acattcaaaa ccacagccgc tacgaaggca gaagcttcgg cataggcggc
     5341 agtttcgacc tgaacggcgg ctgggacggc acggttaccg acaaacaagg caggcctacc
     5401 gacaggataa gcccggcagc cggctacggc agcgacggag acagcaaaaa cagcaccacc
     5461 cgcagcggcg tcaacacccg caacatacac atcaccgacg aagcgggaca acttgcccga
     5521 acaggcagga ctgcaaaaga aaccgaagcg cgtatctaca ccggcatcga caccgaaact
     5581 gcggatcaac actcaggccg tctgaaaaac agcttcgaca aagacgcggt cgccaaagag
     5641 atcaa
//
LOCUS       DAWFHY010000105         5615 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.105, whole genome shotgun sequence.
ACCESSION   DAWFHY010000105 DAWFHY010000000
VERSION     DAWFHY010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5615
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.105"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(230..583)
                     /locus_tag="WGQ68_001856"
                     /pseudo
     CDS             complement(230..583)
                     /locus_tag="WGQ68_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225727.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(667..969)
                     /locus_tag="WGQ68_001857"
     CDS             complement(667..969)
                     /locus_tag="WGQ68_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003750739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715612.1"
                     /translation="MLNEIFEIYSRQGESLIGIGIREAALPVPIAIDILNLFINERIL
                     VLGGDIYIKKDNYFYQTYDNWYYEGSNLFNSIDKAMHYLSQIKLENAYVSFVLKFI"
     gene            complement(1148..1354)
                     /locus_tag="WGQ68_001858"
     CDS             complement(1148..1354)
                     /locus_tag="WGQ68_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715613.1"
                     /translation="MMENSKKTWEIDGEIWLHCPVCGTEVMDYDICDVCQWQNTGETN
                     IDGGPNEMTLAEAKEAYAKGLPIR"
     gene            complement(1320..2069)
                     /gene="mafB"
                     /locus_tag="WGQ68_001859"
                     /pseudo
     CDS             complement(1320..2069)
                     /gene="mafB"
                     /locus_tag="WGQ68_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257312.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
     gene            complement(2495..2839)
                     /locus_tag="WGQ68_001860"
     CDS             complement(2495..2839)
                     /locus_tag="WGQ68_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715614.1"
                     /translation="MIIEHNGNIHKIARMTGNKNNFLEIILSDIHENIKIKPLTIKVK
                     GENVINILPEEVSFYVKQGVDLIYEKYKRKFFISEISFCQSDSRPSSIYAFLTFHLLE
                     DIIKNESPSNYT"
     gene            complement(2877..3221)
                     /locus_tag="WGQ68_001861"
     CDS             complement(2877..3221)
                     /locus_tag="WGQ68_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715615.1"
                     /translation="MFLDDVNVFLDDLNVFLDDLNTNPITDEWYMSNFADKHIKILES
                     YEAFDILKQFVDYMIEEYDEKSEYEIMEILRQLKYQADTNEKFYTNTQKQKIVELYKQ
                     EISQDILNEIFR"
     gene            complement(3224..3412)
                     /locus_tag="WGQ68_001862"
     CDS             complement(3224..3412)
                     /locus_tag="WGQ68_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002240137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mafB"
                     /protein_id="HEZ0715616.1"
                     /translation="MEEVKANPQGKTPARIPPMSDTKNGWLAKDGWVKRVQNVNKIEI
                     HYIENSRTGEKTDFKFKD"
     gene            complement(3482..3817)
                     /locus_tag="WGQ68_001863"
                     /pseudo
     CDS             complement(3482..3817)
                     /locus_tag="WGQ68_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503511.1"
                     /note="MafB polymorphic toxins, described in Neisseria,
                     are fratricidal toxins the provide a competitive
                     advantage. Each MafB is accompanied by an immunity protein
                     MafI.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MafB family polymorphic toxin"
     gene            complement(3923..4294)
                     /locus_tag="WGQ68_001864"
     CDS             complement(3923..4294)
                     /locus_tag="WGQ68_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMI1/KNR4 family protein"
                     /protein_id="HEZ0715617.1"
                     /translation="MIKQNSFVPYPEAMLPKGFKYPQSYLKLAQSTHAINYDEQYSFP
                     WWFENAESNISEVIDIYFEITGIPNLLPFARNQEWAACFDISDKSGNPKIIVVNLDNT
                     KYYETFENFDTWLKEAENDGW"
     gene            complement(4291..4542)
                     /locus_tag="WGQ68_001865"
     CDS             complement(4291..4542)
                     /locus_tag="WGQ68_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715618.1"
                     /translation="MDNTRIMAAREAGVKVEANVHNFNDRLSSKERIRFKHDGIEPQT
                     WGEAIQLRIRKQETQKGVPEGWSKRFPNGSIYDVKVLRK"
     gene            complement(4674..4880)
                     /locus_tag="WGQ68_001866"
     CDS             complement(4674..4880)
                     /locus_tag="WGQ68_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715619.1"
                     /translation="MNILSINNQNSTISLTQDEVFVLRAILNEIYAGVCVDSREFENV
                     SGVRKHEVDNLQQQFAGIYKKMTT"
     gene            complement(4890..5558)
                     /gene="mafB"
                     /locus_tag="WGQ68_001867"
                     /pseudo
     CDS             complement(4890..5558)
                     /gene="mafB"
                     /locus_tag="WGQ68_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 3"
BASE COUNT         1481 a         1116 c          963 g         2055 t
ORIGIN      
        1 gattatttgg caatggattg ccttcataat cttgtcgttg cggatatttt ccctgtacac
       61 catcggttcg cggcactctt tgggttctta taacctctcc atttggacga atatctattt
      121 tcacgccatc aggacctttc catgtttcat attgagcctc atctgaacca gtacgtctaa
      181 aaccttgttc ttgcaatgct tgacgtaatt catctcgcgt agttggtctg atagatttaa
      241 caactggctt gtttcctcct gccatctcac cgtccaacgt ctgcataggt cgggcagcag
      301 gttttttata gccggtattt ttaacccaat cccatttggt cgggttaagg tttacttttt
      361 taccgcccca aaccgtacct gcggcctctg ctatggcaag agcagtttgg gctgttgcag
      421 ttatattcgg atgggcatca gcccattgtc tggcggaatt gatgctgtct tttaccgcaa
      481 aagcactgtc ctgtaatagg ctcgcggcag caagttgtgc ttctggactt aattttccta
      541 aatcattaat accttgtaga gtctgctgag cggctgtatc tgagccggtc agtgtaggtt
      601 ggattaaaac ccagcatttt caacatcttg atgttttact ataaatctat tcttgtgctt
      661 cctttgttag ataaatttca acacaaaaga tacgtatgca ttctctaatt ttatttgaga
      721 taaataatgc attgctttgt cgatactgtt aaataaatta cttccctcgt aataccaatt
      781 atcatatgtt tgataaaaat aattatcttt cttgatataa atatctcccc ccaatacaag
      841 tattctctca ttgataaata aatttaatat atctattgca atagggacgg gtaatgcggc
      901 ttctctaatt ccaattccta tcaaagattc cccttgtctc gaataaattt caaaaatttc
      961 atttaacatg ttaatctcta tctactattt ttcaatcggt ataaacccta ttcacatttt
     1021 atttttccta aaaccaaatc ttcgactaac tgggttgcat aaataatatc atttatgatt
     1081 ttaacaggta aattatttga taggtaacca tgggattccg tcatcattga tttctctagg
     1141 tgcttattta tctgattggt aagccttttg cgtaagcttc tttcgcctcc gcaagtgtca
     1201 tttcattagg tccaccatct atattagttt ctcctgtatt ttgccactga caaacgtcac
     1261 agatatcata gtccataact tcagttccgc aaacaggaca atgtagccat atttctccgt
     1321 caatttccca tgtctttttt gaattctcca tcataatact tctttcctaa gtttggttta
     1381 aacattgttt tgattttgaa tgtttttgga ttggccaaaa catagatatt gttattgata
     1441 tcatagcgca ctcgtctatg ttccgtctga taacctaata tcttgtccga tgtctgtttt
     1501 gataacaaat cagacgccat tttttggtag tcttcaatgg ttatatcgcc aaattctttt
     1561 ccatgagctt tgaaatgtcc atttaaagat ttctcagtag gaaacttgga ctttgtccat
     1621 ctgatgcggt cttgaattgt tcctgtcgga gaaatagatg tatcaggttt aacatatgag
     1681 gcaggtaagc cgatattgtt atcagagtgt gtagggtgtt gctgcgtact tggttttggt
     1741 ggcttattcc cacctgccat ctccccatct aaagtctgca tagggcgggc agtaggtgtt
     1801 ttatagccgg tatttttaac ccaatcccat ttggtcgggt taagttctac ttttttaccg
     1861 ccccaaaccg tacctgcggc ctctgctatg gcaagggcgg tttgggcagt agctgtaatg
     1921 ttcggatggg catcagccca ttgtctggcg gaattgatac cgtcttttac cgcaaaagta
     1981 ctgtcctata atgcgcttgc ggcagcaagt tgtgctttcg gacttaaatt tcctaaatca
     2041 ttaatacctt gtaaagtttg ctgcgcagag atgtaggtcg gatatttgta tccgacaaaa
     2101 atatttgacg catctatcgt ttccgaaatg ccgctgttgg aaaatgtcgg attcgagaat
     2161 ccgacctacg gctatttcgt ttaagtcata aatagccatt catgttcctt ctgaatttgc
     2221 ttttgctatc cttagaacca atcatataaa cattatcatc attaattaat gttctaagtt
     2281 tatgaagcgg tcaaatctgt caaactacca gagatgaagc atatcctccg ggaagtaatt
     2341 ctgttcgtgc tgtttccata tcttctttgc catcatcagt tacttctctg tctaaataaa
     2401 atcttacgat aagtttttgt ctaatttcat caaattctgc cgtaattaat cttatattga
     2461 tatgaataca cggttcatac ctgctattag ccagtcaggt gtagttggat ggggattcat
     2521 ttttaataat atcttcaagc aagtgaaatg taagaaaagc gtagatactt gaaggccggc
     2581 tatctgattg gcaaaaagaa atttcggaga taaagaattt ccgtttatat ttttcataaa
     2641 ttaaatcaac accttgtttt acataaaaac taacttcctc aggaaggata tttataacat
     2701 tctctccttt tacttttata gttaatggtt tgatttttat gttttcatga atatctgata
     2761 ggattatttc taaaaaatta tttttatttc cagtcattct ggctatttta tgtatatttc
     2821 cattgtgttc gattatcatt ttattttttc caaggatttc cttctatatt gatagtttat
     2881 ctaaagattt catttaaaat atcctgacta atttcttgtt tatataattc tacaattttc
     2941 tgtttctgtg tatttgtata aaatttttcg ttggtatctg cttgatattt taattgtctc
     3001 aatatttcca tgatttcata ttctgatttt tcatcatatt cttcaatcat gtaatcaaca
     3061 aattgtttta gaatatcaaa ggcttcgtaa ctttccaaaa ttttaatatg tttatcggca
     3121 aaattggaca tataccattc gtcagtgatt ggattagtat ttaaatcatc taaaaaaaca
     3181 tttaaatcat ctaaaaaaac atttacatca tctaaaaaca tgactaatcc ttaaacttaa
     3241 aatctgtttt ctcaccggtt cttgagtttt caatgtaatg tatttcaatt ttgtttacgt
     3301 tttgaacacg cttaacccaa ccgtcttttg ctaaccaacc atttttagta tcggacatag
     3361 gaggtattct cgcaggagtt ttgccctgtg ggtttgcctt aacttcttcc attgctaatt
     3421 gttcctttaa attattaggt attgtacgac cggttgattt ttcaacagat tgtgtcccta
     3481 tttttaatga tttgtttccc ccagccattt ccccatctaa agtctgcata gggcgggcag
     3541 caggtttttt ataaccggta tttttaaccc aatcccattt ggtcgggtta aggtttactt
     3601 ttttaccgcc ccaaaccgta cctgcggcct ctgctatggc aagagcagtt tgggctgttg
     3661 cagttatatt cggatgggca tcagcccatt gtctggcgga attaatgccg tcttttaccg
     3721 caaaagcact gtcctgtaat aggctcgcgg cagcaagttg tgcttccgga cttaaatttc
     3781 ctaattcatt aatatcttgt aaagtttgct gcgcagagat gtaggtcgga tatttgtatc
     3841 cgacaaaaat atttgacgca tctatcgttt ccgaaatgcc gctgttggaa aatgtcggat
     3901 tcgagaatcc gacctacggc tgctaccaac catcattttc agcttctttt agccaagtat
     3961 caaaattttc aaaagtctcg taatattttg tattatctaa attaactact ataattttag
     4021 gattacctga tttatctgaa atatcaaaac aggcagccca ctcttggttt ctagcaaaag
     4081 gtaataggtt tggaatgcca gttatttcaa aataaatgtc aattacttct gatatattgc
     4141 tttctgcatt ttcaaaccac caaggaaaag aatattgttc atcgtagtta atggcatgag
     4201 tggattgagc taattttaaa taactttgcg gatatttaaa tcctttagga agcattgctt
     4261 caggatacgg aacaaaacta ttttgtttta tcatttccta agtaccttta catcataaat
     4321 acttccgtta ggaaatcttt tgctccaccc ttctggaact cctttttgtg tttcttgctt
     4381 tctaattcgt agctggatag cttctcccca agtttgaggc tcaataccat catgcttaaa
     4441 cctgattctc tcttttgatg ataatcggtc attaaaatta tgaacattcg cttccacttt
     4501 tactcctgct tcacgtgctg ccataatacg cgtgttatcc atactagtag gtgctccatc
     4561 aggcatatta acaacatcaa ccctgtcgcc aacccatgat ttttttcttt catttcggct
     4621 acaatagaat catagttgta atttgcacta ggctctgaca aaaatttggg ttgttaagtt
     4681 gtcatttttt tataaattcc agcaaactgt tgttgtaaat tatctacttc atgttttcta
     4741 acaccagata cattttcaaa ttctcttgaa tctacacata cgcccgcata tatctcattc
     4801 aagatagctc gtaaaacaaa aacttcatct tgagttagtg aaatagttga gttttgatta
     4861 tttatggata gtatattcat ggctttttcc tataaatcat cataatattt tttacctgat
     4921 gttggtctaa atgctgtacc tccatcgtca gaatttttat ctcggataac tattgtccct
     4981 gttttatcat cccaatacgc agttctaccg cgaggtaact ctttcatatt tgttggatgg
     5041 ctaacaatat tttcaatatg ccgagcaaaa tctgctggtg aattgatatt taaatccgta
     5101 aattcttgtt gtcttatgac atgcttgtta taagcatgcc cactactaat ttgttctcca
     5161 attagttgcg cttgtaaaga cggttgtgtg gaagcatctg ctttgctgtt ggacgttata
     5221 gatttaggcg gtctattccc ccctgccatc tccccatcta cagtctgcat atggcgggta
     5281 gcaggttttt tataatcggt atttttaacc caatcccatt tagtcgggtt aagttctact
     5341 tttttaccgc cccaaaccgt agttgcggcc tctgctgcgg caagagcagt ttgggctgtt
     5401 gcagttatat tcggatgggc atcagcccat tgtctggcgg aattaatgcc gtcttttacc
     5461 gcaaaagcac tatcctgtaa taggctcgcg gcagcaagtt gtgtttccgg acttaaattt
     5521 cctaaattat taataccttg tagagtctgc tgcgcagaga tgtagcaact gtatttttac
     5581 cccgtcgggt aaaaatacag ttgctacggc tgctg
//
LOCUS       DAWFHY010000106         5556 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.106, whole genome shotgun sequence.
ACCESSION   DAWFHY010000106 DAWFHY010000000
VERSION     DAWFHY010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5556
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.106"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(335..3166)
                     /gene="lbpA"
                     /locus_tag="WGQ68_001868"
                     /gene_synonym="tbpA"
     CDS             complement(335..3166)
                     /gene="lbpA"
                     /locus_tag="WGQ68_001868"
                     /gene_synonym="tbpA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoferrin/transferrin-binding protein LbpA"
                     /protein_id="HEZ0715620.1"
                     /translation="MNKKHGFQLTLTALAVATAFPAYAAQAGGAAPDAAQTQSLKEVT
                     VRAAKVGRRSKEATGLGKIVKTSETLNKEQVLGIRDLTRYDPGVAVVEQGNGASGGYS
                     IRGVDKNRVAVSVDGVAQIQAFTVQGSLSGYGGRGGSGAINEIEYENISTVEIDKGAG
                     SSDHGSGALGGAVAFRTKEAADLISDGKSWGIQAKTAYGSKNRQFMKSLGAGFSKDGW
                     EGLLIRTERQGRETRPHGDIADGVAYGINRLDAFRQTYDIKRKTREPFFSVEGERESK
                     PVAKLAGYGKYLNNQLNRWVKERIEQNQPLSAEEEAQVREAQARHENLSAQAYTGGGR
                     ILPDPMDYRSGSWLAKLGYRFGGRHYVGGVFEDTKQRYDIRDMTEKQYYGTDEAEKFR
                     DKSGVYDGDDFRDGLYFVPNIEEWKGDKKLVRGIGLKYSRTKFIDEHHRRRRMGLLYR
                     YENEAYSDNWADKTVLSFDKQGVATDNNTLKLNCAVYPAVDKSCRASADKPYSYDSSD
                     RFHYREQHNVLNASFEKSLKNKWTKHHLTLGFGYDASNAISRPEQLSHNAARISESTG
                     FDDNNQDKYRLGKPEVVEGSVCGYIETLRSRKCVPRKINGSNIHISLNDRFSIGKYFD
                     FSLGGRYDRKNFTTSEELVRSGRYVDRSWNSGIVFKPNRHFSVSYRASSGFRTPSFQE
                     LFGIDIYHDYPKGWQRPVLKSEKAANREIGLQWKGDFGFLEISSFRNRYTDMIAVADH
                     KTKLPDSTGRLTEIDIRDYYNAQNMSLQGINILGKIDWNGVYGKLPEGLYTTLAYNRI
                     KPKSVSNRPGLSLRSYALDAVQPSRYVLGFGYDQPEGKWGANIMLTYSKGKNPDELAY
                     LAGDQKRYSTKRASSSWSTADVSAYLNLKKRLTLRAAIYNIGNYRYVTWESLRQTAES
                     TANRHGGDSNYGRYAAPGRNFSLALEMKF"
     gene            complement(3163..5340)
                     /locus_tag="WGQ68_001869"
     CDS             complement(3163..5340)
                     /locus_tag="WGQ68_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin-binding protein-like solute binding
                     protein"
                     /protein_id="HEZ0715621.1"
                     /translation="MCKPNYGGIVLLPLLLASCIGGNFGVQPVVESTPTAYPVTFKSK
                     DVPTSPPAGPSIETTPVNRPAVGAAMRLLRRNTAFHREDGTAIPNSKQAEEKLSFQEG
                     DVLFLYGSKGNKLQQLKSEIHKRDSDVEIRTSEKENKKYDYKFVDAGYVYVKGKDEIK
                     WTSDRKQFSNRLGYDGFVYYSGERPSQSLPSAGTVEYSGNWQYMTDVKRHRAGKAVGI
                     DNLGYITFYGNDVGATSYAAKDVDEREKHPAKYTVDFDQKTLNGKLIKNQYVQRKTDE
                     KKPLTIYDITAKLDGNRFTGSAKVNPDLAKNPAGNERLFFHADADQRLEGGFFGDNGE
                     ELAGRFISNDKSVFGVFAGKQKTETANASDTNPALPFGKHTKILDSLKISVDEATDSN
                     ARKFAISSMPDFGHPDKLLVEGREIPLVSQEKTIKLADGRKMTVSACCDFLTYVKLGR
                     IKTDRPAAKPKAQDEDSEDEIGESEENEDEISDEEGTEDEVVEDEGSEEDEVSEGDNG
                     ENEEEIAEEETEEEVDEAEEEKVEEPEEELPAEEGNGGSGSILPTPEAPKGRDIDLFL
                     KGIRTAEADIPKTGKAHYTGTWEARIGVPDKKDDQPGTTFIQKDSYANQGAKAEFDVD
                     FGAKSLSGKLTEKNDTHPAVYIENGVIDGNGFHATARTRDNGIDLSGQGSTSPQSFKA
                     NNLLVTGGFYGPQAAELGGNIIDSDRKFGAVFGAKKDNKEVEK"
BASE COUNT         1201 a         1501 c         1276 g         1578 t
ORIGIN      
        1 tttttgagtt tcagtcattt ccgataaatt gccttagtat tgaatgtcta gattcccgcc
       61 tgcgcgggaa tgacgaatcc atccatacgg aaacctgcat cccgtcattc ccacgaacct
      121 acatcccacc attcccacaa aagtgggaat ccagtttttc gagtttcagt cattcccgat
      181 aaattgcctt agcattgaat gtctagattc ccgcctgcgc gggaatgacg acggaaatct
      241 tgtttatatt gaatcaaaaa aacctgcacc ttaatcagtt ggcggtttag tccgactttt
      301 ggggtgcaga tcaagctttc agacggtatt tcctttaaaa cttcatttcg agcgcgaggc
      361 tgaagtttct gcccggtgcg gcataccttc catagttgct gtcgccgccg tgccggtttg
      421 ccgtgctttc cgcggtctgg cgcaaggatt cccaagtaac gtagcggtag ttgccgatat
      481 tgtagatagc cgccctcaag gtcagccgtt ttttcagatt cagataggcg gaaacgtctg
      541 ccgtcgacca agaagacgac gctctttttg tcgaatatcg tttttgatcg cctgccagat
      601 aagcaagctc gtcagggttt ttccctttgg aataggtcag cataatgttt gcgccccatt
      661 tcccctcggg ctggtcgtat ccgaacccca aaacataacg cgacggctgt accgcatcca
      721 aagcatagct gcggagggac agtcccggcc ggttggatac cgatttcggt ttgatacggt
      781 tgtacgccaa agtcgtgtac aaaccttcgg gcagtttgcc gtacacgccg ttccagtcga
      841 tttttcccaa tatattaatg ccttggagcg acatattttg ggcattgtaa taatcgcgta
      901 tatcaatctc tgtcaatcgt cctgttgaat ccggcaattt ggttttgtga tcggcaacgg
      961 caatcatatc ggtatagcgg ttgcggaagc tgctgatttc caaaaagccg aaatcgccct
     1021 tccactgcaa accgatttcg cggttggctg ccttttccga tttcaggacg ggacgctgcc
     1081 agcctttcgg ataatcgtga taaatgtcta taccgaaaag ttcttggaat gagggcgttc
     1141 tgaagccgct ggaggcgcgg taagacacgg aaaaatgccg gttcggtttg aacacgatgc
     1201 cgctgttcca cgaacggtca acataccgtc cgctgcggac gagttcttcc gacgtggtga
     1261 agtttttccg gtcgtacctg ccacccaagc tgaaatcaaa atatttgccg attgaaaaac
     1321 ggtcgttcaa agaaatatgg atattgctgc cgttgatttt tcttggcacg catttgcggg
     1381 aacgcagggt ttcgatgtag ccgcagaccg acccttcgac gacttcgggc ttacccaaac
     1441 ggtacttatc ttgattgtta tcatcgaatc ccgtggattc cgaaatcctt gccgcattgt
     1501 gggaaagctg ttcggggcgg gagatcgcgt tggaagcatc gtaaccgaag cccaaagtca
     1561 gatggtgttt cgtccatttg tttttcagcg atttctcaaa cgaggcattc aaaacattgt
     1621 gctgttcacg gtagtggaaa cggtcgctgc tgtcgtagga atacggtttg tccgccgacg
     1681 cgcggcagga tttgtccaca gcaggataca cggcgcaatt cagcttcagc gtgttgttgt
     1741 cggttgccac gccctgtttg tcaaacgaca acaccgtctt gtccgcccaa ttgtcagaat
     1801 acgcttcgtt ttcataacgg tacagcaaac ccatacggcg gcggcggtga tgttcgtcaa
     1861 taaatttggt gcgggaatat ttcaaaccta tgcccctgac caacttttta tcgcccttcc
     1921 actcttctat attcggcaca aaatacaagc cgtcgcggaa atcgtcgccg tcgtacaccc
     1981 cgctcttgtc tctaaacttt tccgcctcgt ccgtaccgta atactgtttt tccgtcatat
     2041 cgcggatgtc gtaacgctgt ttggtatcct caaacacgcc gccgacataa tgcctgccgc
     2101 cgaagcggta gcccagcttg gcaagccaag agccgctgcg gtaatccatc ggatcgggca
     2161 atatcctgcc gccgcccgtg taagcttggg cggacagatt ttcgtggcgc gcctgcgcct
     2221 cccgcacctg cgcctcttct tcagcactta aaggctgatt ttgttcaata cgttctttta
     2281 cccagcggtt gagctggttg ttcaaatatt tcccgtagcc cgccaatttt gccacgggct
     2341 tggattcacg ctcgccctct actgagaaaa atggctctct tgtcttgcgt ttaatatcgt
     2401 atgtctggcg gaacgcgtcc aaacggttta tgccgtatgc caccccgtcc gcaatatcgc
     2461 cgtgcgggcg cgtttcccgc ccctgacgtt cggttcggat tagcagccct tcccaaccgt
     2521 ctttgctgaa ccccgcgccg agcgacttca taaattggcg gtttttactg ccgtaggcag
     2581 tttttgcctg tatcccccag cttttgccgt ctgaaatcag gtctgccgcc tctttggtgc
     2641 ggaaggcgac tgcgccgccg agtgcgccgc tgccgtgatc ggacgaaccg gcacctttgt
     2701 cgatttccac cgtgctgatg ttttcatatt cgatttcgtt gattgcaccg ctgccgccgc
     2761 gtccgccgta tccgctcaac gatccctgca cggtaaacgc ctgtatttgg gcaacgccgt
     2821 caaccgaaac cgccacacgg tttttatcca cgccgcgtat cgagtagccg ccgctcgcgc
     2881 cgttgccctg ttcgacaacc gccacgcccg gatcgtagcg cgtcaggtcg cggataccga
     2941 gtacctgttc tttgttcaac gtttccgacg ttttgacgat tttgcccaaa ccggtcgcct
     3001 ctttcgatcg ccgtcccact ttggcggcac ggacggtaac ctctttcaag gattgggtct
     3061 gcgccgcatc aggtgccgcc ccccccgctt gggcagcata agccggaaaa gcggttgcaa
     3121 cagccaaggc agtcagggta agctgaaaac cgtgtttctt attcattttt ccacctcttt
     3181 attatctttc ttcgcaccga ataccgcgcc gaatttccgg tcgctgtcga taatattacc
     3241 gcccaattcc gccgcctgcg gtccataaaa gccgcctgtt acaagaagat tgttggcttt
     3301 gaagctctgc gggctggtcg aaccctgccc agaaaggtca ataccgttat cccgagtgcg
     3361 tgctgtcgcg tggaaaccgt tgccatcaat cacaccgttt tcaatataaa cagcggggtg
     3421 tgtatcattt ttttctgtca acttacctga aagcgacttc gcgccgaaat caacgtcaaa
     3481 ttctgctttt gccccttgat tcgcatagct atccttttga atgaacgtag tgccaggctg
     3541 atcgtctttc ttatctggta cgccgatacg cgcttcccaa gtgccggtat agtgtgcttt
     3601 tccagttttt ggaatgtcgg cttccgccgt gcggatacct ttcaggaaaa ggtcgatgtc
     3661 cctgccttta ggggcttccg gagtgggcag gatgctgcct gaaccgccgt tgccttcttc
     3721 tgccggcaat tcttcttcgg gttcttcaac tttctcctct tcggcttcat caacttcttc
     3781 ttcggtttct tcttcggcga tttcttcttc gttttcaccg ttatcacctt cggaaacttc
     3841 gtcttcttcg ctgccttcat cttctacgac ttcatcttcc gtgccttctt catcgctgat
     3901 ttcgtcttcg ttttcttcgc tttcaccaat ttcatcttcg gaatcttcgt cctgtgcctt
     3961 tggtttggcg gcggggcggt cggtttttat ccgtccgagt ttcacatagg tcagaaaatc
     4021 gcaacaagca ctgacggtca ttttcctgcc gtcggcaagc ttgatggttt tctcttggct
     4081 aaccaaagga atttcacgcc cttcgacaag aagcttgtcg ggatgaccaa aatcgggcat
     4141 agaggaaatg gcaaacttac gggcattgct atcagtcgcc tcgtcaacgg aaatttttag
     4201 agaatccaag attttggtgt gttttccaaa cggcagggca ggatttgtat ctgatgcgtt
     4261 tgctgtctct gttttttgtt tgcctgcgaa tacgccgaat acgcttttgt cgttgctgat
     4321 aaatcgtccg gcaagctctt ctccgttatc gccgaaaaaa ccgccctcaa gccgctgatc
     4381 ggcatcggca tggaaaaaca aacgctcatt accggcaggg tttttcgcta aatcaggatt
     4441 gaccttggca ctgccggtaa agcggttgcc gtccaatttt gcagtaatgt cgtaaatggt
     4501 cagcggtttc ttttcatcgg ttttcctttg cacatactga tttttaatca gcttgccatt
     4561 cagagttttt tggtcgaaat caaccgtata tttggcagga tgcttttccc tttcgtcgac
     4621 atccttagcc gcataagaag ttgcaccaac atcgttacca taaaatgtga tataacccaa
     4681 attgtcaatg ccaaccgcct tacctgctcg atgacgtttg acatcggtca tatattgcca
     4741 gttaccagaa tattccaccg ttcccgcact cggtaaagat tgggaaggac gttctccgga
     4801 ataatataca aaaccgtcat aacctaagcg gttggaaaac tgcttgcgat ctgaagtcca
     4861 cttaatttca tcttttccct ttacatatac ataacctgca tctacaaatt tataatcata
     4921 ttttttattt tccttttctg atgtcctaat ttctacatcg gaatcacgtt tatgaatttc
     4981 gcttttaagt tgttgaagtt tatttccttt tgaaccgtat aaaaacagaa catcaccttc
     5041 ttgaaacgac agcttttctt ctgcttgttt gctattggga attgccgtgc catcttcacg
     5101 atgaaaagca gtattccgcc tcaacagccg cattgccgca ccgacggcgg gccggttgac
     5161 cggcgtggtt tctatagaag gcccggcagg gggcgaagtg ggaacgtcct tagacttgaa
     5221 agtgacgggg tacgcggtcg gcgttgattc gacaacaggc tgcacgccga aattgccgcc
     5281 aatacaagat gccaaaagta agggcaacaa gacaatgccg ccataattcg gtttacacat
     5341 ccctactttt cctcctattt gattaataat aattatcatt atattaatat gtacagataa
     5401 tatcaagccg tttttatagt gaattaacaa aaatcaggac aaggcgacga gccgcagaca
     5461 gtacagatac atcccgtcat tcccacgaac ctgcatcccg tcattcccac gaacctacat
     5521 tccgtcattc ccacgaacct gcaccacgtc attccc
//
LOCUS       DAWFHY010000107         5515 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.107, whole genome shotgun sequence.
ACCESSION   DAWFHY010000107 DAWFHY010000000
VERSION     DAWFHY010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5515
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.107"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(128..1639)
                     /gene="lysS"
                     /locus_tag="WGQ68_001870"
     CDS             complement(128..1639)
                     /gene="lysS"
                     /locus_tag="WGQ68_001870"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009313040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEZ0715622.1"
                     /translation="MSEQNHPQTEPQLDENQIIALRREKLHNIRQQRNAYPNDFKRDS
                     FAADLHAQYGEIGKEELDPQGIPVKVAGRMMLKRQMGKASFATIQDVSGQIQLYLNNK
                     GVSQEVLDDFNHWDLGDIVGAEGTLFKTNHGELTVRVSGIRLLSKSLRPLPDKHKGLS
                     DQETKYRQRYVDLIANEESRNTFIKRSQIIQSVRNFMVGEHYLEVETPMMHPIPGGAT
                     AKPFVTHHNALDIPLYLRIAPELYLKRLVVGGLERVFEINRSFRNEGMSVRHNPEFTM
                     IEFYEAFSDYERMMQMAEDIIRNASHTVNGTANITYNGKEVDLESPFERLTILEAIKK
                     YNPHYTDEQLNDAEWLKKEIVKHGESLPPSPGIGSLQLALFEGCAEGKLWNPTFIVDY
                     PVEVSPLARASDTKQGLTERFELFVVGRELANGYSELNDPEDQAERFKAQVAQKDAGD
                     DEAMHYDADYIRAMEFGLPPTGGCGIGIDRLVMLLTDSQTIRDVILFPQMRPE"
     gene            complement(1636..1788)
                     /locus_tag="WGQ68_001871"
     CDS             complement(1636..1788)
                     /locus_tag="WGQ68_001871"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715623.1"
                     /translation="MPSETANRFQTALPRLWKKMPTSRKIRANFVIRSGRLKQIAPDL
                     FFRKTL"
     gene            complement(1794..3047)
                     /locus_tag="WGQ68_001872"
     CDS             complement(1794..3047)
                     /locus_tag="WGQ68_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEZ0715624.1"
                     /translation="MSEQHISTWKSKINALGPGIMMASAAVGGSHLIASTQAGALYGW
                     QIALIIILTNLFKYPFFRFSAHYTLDTGKSLIEGYAEKSRVYLWVFLILCILSATINA
                     GAVAIVTAAIVKMAIPSLMFDAGTVAALIMASCLIILVSGRYRALDRVSKIIIVTLSI
                     ATLAAAGIAMSRGMQMQSDFIEPTPWTLAGLGFLIALMGWMPAPIEISAINSLWVTEK
                     QRINPSEYRDGIFDFNVGYIASAVLALVFLALGAFVQYGNGEAVQMAGGKYIGQLINM
                     YAVTIGGWSRPLVAFIAFACMYGTTITVVDGYARAIAEPVRLLRGKDKTGNAEFFAWN
                     IWVAGSGLAVIFWFDGVMANLLKFAMIAAFVSAPVFAWLNYRLVKGDEKHKLTSGMNA
                     LALAGLIYLTGFTVLFLLNLAGMFK"
     gene            complement(3108..3308)
                     /locus_tag="WGQ68_001873"
     CDS             complement(3108..3308)
                     /locus_tag="WGQ68_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715625.1"
                     /translation="MHFFGSNTENIVKFEHEVLIVCVGRYLDYVTIRAFIKISLPGGI
                     LFLKTVGFGFVWGQSRPNWSNR"
     gene            3505..5301
                     /locus_tag="WGQ68_001874"
     CDS             3505..5301
                     /locus_tag="WGQ68_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017146933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEZ0715626.1"
                     /translation="MNTVSNYLSALREAMKAQGLDALVIPSADPHLSEYLPEHWQARR
                     ELSGFTGSVGTFVLTTDEAGVWVDSRYWEQAAKQLAGSGIELQKSGQVPPYNEWLAAN
                     LPENAAVGIPSDMVSLTGKRTLAQSLTAKNIRIEHPDDLLDQVWTSRPAIPAETVFVH
                     DPAYVSETAAEKLARVRAVMAEKGADYHLVSSLDDIAWLTNLRGSDVPFNPVFVSYLL
                     IGKDNAVLFTDRCRLNAEAAAALQTAGITVEPYAQVADKLAQIGGALLIEPNKTAVST
                     LVRLPESVRLIEGINPSTLFKSCKSEADIARIREAMEHDGAALCGFFAEFEDIIGNGG
                     SLTEIDVDTMLYRHRSVRPGFISLSFDTIAGFNANGALPHYSATPESHSTISGNGLLL
                     IDSGAQYKGGTTDITRVVPVGTPTAEQKRDNTLVLKAHIALAEAVFPENIPSPLIDAI
                     CRKPLWQAQCDYGHGTGHGVGYFLNVHEGPQRIAFAAPATPETAMKKGMVTSIEPGLY
                     RPGKWGIRIENLAANQAVATPQETEFGSFLCFETLTLCPIDTRLMDTALMTDGEIDWV
                     NRYHAEVRRRLEPLTEGAAKAWLIKRTEPLAR"
BASE COUNT         1291 a         1609 c         1414 g         1201 t
ORIGIN      
        1 atttgtactg tctgcggctc gccgccttgt cctgattttt gttaattcac tatcaatcct
       61 gccatacaaa atgccgtctg aatccaatcg ggttcagacg gcattgccat ttcaactgtt
      121 tttatgatta ctcggggcgc atctgcggga acagaatcac gtcacggatg gtttgcgaat
      181 cggtcagcaa cattaccaag cggtcgatgc cgataccgca accgccggtc ggcggcagac
      241 cgaactccat cgcgcggatg tagtcggcat cgtagtgcat cgcttcgtcg tcgcccgcgt
      301 ctttttgcgc cacttgcgct ttgaagcgtt cggcttggtc ttcggggtcg ttcaactcgg
      361 aatagccgtt tgccagttcg cgcccgacaa cgaacaattc gaaacgttcg gtcagacctt
      421 gtttggtatc cgaagcgcgc gccaacgggg aaacttcgac cgggtaatcg acgatgaagg
      481 tcggattcca cagcttgccc tcggcgcaac cttcaaacag cgcgagttgc aggctgccga
      541 tgcccgggga cggcggcagg ctttcgccgt gtttgacgat ttcttttttc agccattccg
      601 catcgttcaa ctgctcgtcg gtgtagtgcg gattgtattt tttgatggct tcgagaatgg
      661 tcaggcgttc aaacgggctt tccaaatcga cttctttgcc gttgtaagtg atgtttgccg
      721 tgccgtttac cgtgtgcgat gcgttgcgga tgatgtcttc cgccatctgc atcatgcgtt
      781 cgtagtcgga gaaggcttcg tagaattcga tcatggtgaa ttcggggttg tggcgcacgg
      841 acatgccttc gttgcggaag ctgcggttga tttcaaacac gcgttccaaa ccaccgacaa
      901 ccaggcgttt cagatacagc tcgggggcga ttcgcaaata aagcggaata tctaaggcat
      961 tgtgatgggt aacgaagggt tttgccgtcg cgccgccggg aatcgggtgc atcatcgggg
     1021 tttcgacttc gagataatgc tcgcccacca taaaattacg cacggattgg atgatttggc
     1081 tgcgtttgat gaaggtattg cgcgattctt cgttggcaat caaatcgacg tagcgttgac
     1141 ggtatttggt ttcctgatcg ctcaagcctt tatgtttgtc cggcagaggg cgcagggatt
     1201 tggacagcag gcggatgccg gacacgcgta cggtcagttc gccgtggttg gttttgaaca
     1261 aagtgccttc cgcgccgacg atgtcgccca aatcccaatg gttgaagtcg tccaaaactt
     1321 cttggctcac gcctttgttg ttcagataaa gctggatttg cccggacacg tcttgaatgg
     1381 tggcaaagct cgccttgccc atttgacgct tcagcatcat gcggccggcc actttgacgg
     1441 gaatgccttg cggatcgagt tcttctttgc cgatttcgcc gtattgggcg tgcaaatcgg
     1501 cggcgaagct gtcgcgtttg aagtcgttgg gataggcgtt gcgctgttgg cggatgttgt
     1561 gcagtttttc gcggcgcagg gcgatgattt ggttttcgtc caactgcggc tcggtttgcg
     1621 gatggttttg ttcgctcata gggttttccg aaaaaataaa tcaggcgcaa tctgtttcag
     1681 acgacctgac cgaatcacaa aatttgcgcg tattttacgc gatgtcggca tttttttcca
     1741 taaacgcggc aatgccgtct gaaagcggtt tgcggtttca gacggcatcg ttatcatttg
     1801 aacattcccg ccaaattcaa taagaacaaa acggtaaaac cggtcagata aatcaagcct
     1861 gccaatgcaa gggcattcat acctgatgtg agtttgtgtt tttcatcacc tttgaccaaa
     1921 cggtaattca gccaggcaaa cacaggggcg gacacaaaag cggcaatcat cgcaaatttg
     1981 agcagattcg ccattacgcc gtcaaaccag aaaatcaccg ccaaaccgct gcccgccacc
     2041 caaatattcc aggcaaagaa ttcggcgttg cccgttttgt cttttccgcg cagcaggcgc
     2101 acgggttcgg caatggcacg ggcatagccg tccacaacgg taatcgtcgt gccgtacata
     2161 caggcaaacg cgataaacgc caccagcggg cgcgaccagc cgccgatggt aacggcgtac
     2221 atattgatca attgcccgat atatttgccg cccgccatct gcactgcttc gccgttgccg
     2281 tattgcacaa acgcgcccag tgcaaggaaa accaaagcca aaaccgcact ggcgatataa
     2341 ccgacgttga aatcaaaaat cccgtcgcgg tattcggaag gattgatgcg ttgtttttcg
     2401 gttacccaca aagaattgat ggcggaaatt tcaatcggcg cgggcatcca gcccatcagc
     2461 gcgatcagga agcccaaacc ggcaagcgtc cacggtgtcg gctcgataaa atcggactgc
     2521 atctgcatac cgcgcgacat agcgatgccg gcggcggcaa gcgtggcgat actcaaagta
     2581 acgatgatga ttttggaaac gcgatccaaa gcgcggtaac gtccgctcac caaaataatc
     2641 aggcaggatg ccataatcaa ggcggcaacc gtgccggcat caaacatcag cgagggaatc
     2701 gccattttga cgatggcggc ggttacaatg gcgaccgcgc ccgcgttaat cgtggcggag
     2761 aggatgcaca aaatcaggaa tacccacaaa taaacgcggc ttttctcggc ataaccttca
     2821 atcaggctct tgcccgtgtc cagcgtgtaa tgcgcgctga agcggaaaaa cgggtatttg
     2881 aagaggttgg tcaggatgat gatgagcgcg atctgccagc cgtaaagcgc gcccgcctgc
     2941 gtcgaggcaa tcaggtgcga accgccgacc gccgccgaag ccatcataat ccccggtccc
     3001 aatgcgttga ttttactttt ccaagtcgaa atatgttgtt cggacataaa gtcttccgta
     3061 tttttaaccg tgtttcaaca cacagagccg catattcgga cacagcccta tctattgctc
     3121 caatttgggc gggattgccc ccaaacaaac ccaaatccta ccgtcttcaa aaacaggata
     3181 ccgcccggta gggaaatttt gatgaaagcg cgtattgtaa cgtaatccaa atacctgcca
     3241 acacacacta ttagaacttc atgctcaaac ttgactatat tttccgtatt acttccaaaa
     3301 aaatgcataa aacgatatct tatgcctaaa attttacaac aaacaacctt acatcgcttt
     3361 tttcgcgcaa acacgcacca tccgatcagc ccgtccgttt tgcagcaggc tggcgatttg
     3421 ataagatggt tatgtttttc agacggcatt tcagatttcc gtccatgccg tctgaagccg
     3481 caaaacccga ttggaggaac tgttatgaat accgtatcga attacctgtc cgcattgcgc
     3541 gaagccatga aggcgcaagg cttggatgca ctcgtcatcc cttccgccga cccccacctg
     3601 tccgaatacc tgcccgagca ttggcaggcg cgccgcgaat tatcgggctt taccggctcg
     3661 gtcggcacgt ttgtcctgac caccgatgaa gcgggcgtat gggtggacag ccgctattgg
     3721 gaacaagccg ccaagcagct tgcgggcagc ggcatcgaac tgcaaaaaag cgggcaagtg
     3781 ccgccgtata acgaatggct cgcggcaaac ctgcccgaaa acgccgccgt cggcatccct
     3841 tccgatatgg tctcgctcac cggcaaacgc actttggcgc aatccctgac cgccaaaaac
     3901 atccgcatcg aacacccgga cgatctgctt gaccaagtgt ggacaagccg tcccgctatc
     3961 cccgccgaaa ccgtgttcgt ccacgacccc gcctacgtct ccgaaaccgc cgccgaaaaa
     4021 ctcgcccgcg tgcgcgccgt gatggcggaa aaaggcgcgg attaccactt ggtttcctcg
     4081 ctcgacgaca tcgcctggct gaccaacctg cgcggcagcg acgtgccttt caatcccgtt
     4141 ttcgtgtcct atctattgat tggcaaagac aacgccgtcc tgtttaccga ccgatgccgt
     4201 ctgaatgccg aagccgccgc cgcgctgcaa accgcaggca tcacggtcga gccttacgcc
     4261 caagttgccg acaaactcgc gcaaatcggc ggcgcgttgc ttatcgaacc gaacaaaacc
     4321 gccgtcagca cgcttgtgcg cctgcccgaa agcgtgcgcc ttatcgaggg aatcaacccg
     4381 tccacgctgt tcaaatcctg caaatccgaa gccgacatcg cccgcatccg cgaagcgatg
     4441 gaacacgacg gcgcggcgtt gtgcggtttc ttcgccgagt ttgaagacat catcggcaac
     4501 ggcggcagcc tgaccgaaat cgacgtggac accatgcttt atcgccaccg cagcgtgcgc
     4561 ccaggcttca tttcattgag tttcgacacc atcgcaggct tcaacgccaa cggcgcactg
     4621 ccgcattaca gcgcaacgcc cgaaagccac agcaccatca gcggcaacgg gcttttgctc
     4681 atcgactccg gcgcgcaata caaaggcggc acgaccgaca tcacccgcgt cgtccctgtc
     4741 ggcacaccca ccgccgaaca aaaacgcgac aacaccctcg ttctcaaagc ccatatcgcg
     4801 cttgccgaag ccgtgttccc cgaaaacatc ccctcgccgc tgattgatgc gatttgccgc
     4861 aaacccctgt ggcaggcgca atgcgactac ggccacggca ccggacacgg cgtaggctat
     4921 ttcctcaacg tccacgaagg cccgcagcgc atcgccttcg ccgcccccgc cacgcccgaa
     4981 accgccatga aaaaaggcat ggttacctcc atcgaacccg gactctaccg cccgggaaaa
     5041 tggggcatcc gcattgaaaa ccttgccgcc aaccaagccg tcgccacccc tcaagaaacc
     5101 gaattcggca gcttcctctg ttttgaaacc ctgaccctct gccccatcga tacacgcctg
     5161 atggacaccg ccctcatgac cgacggcgaa atcgactggg tcaaccgcta ccacgccgaa
     5221 gtccgccgcc gcctcgagcc gctgaccgaa ggcgcggcaa aagcgtggct gatcaaacgc
     5281 accgaaccgc tggcgcgtta aacagcacgg cgcaaaaaat gccgtctgaa agcccttcag
     5341 acggcattgg tttcccaaaa catcccgcac cgttttcatc ttgccgcaag caaatatagt
     5401 ggattaacaa aaatcaggac aaggcgacga agccgcagac agtacgaata gtacggaacc
     5461 gattcacttg gtgcttcagc accttagaga atcgttctct ttgagctaag gcgcg
//
LOCUS       DAWFHY010000108         5446 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.108, whole genome shotgun sequence.
ACCESSION   DAWFHY010000108 DAWFHY010000000
VERSION     DAWFHY010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.108"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(78..278)
                     /locus_tag="WGQ68_001875"
     CDS             complement(78..278)
                     /locus_tag="WGQ68_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002239016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715627.1"
                     /translation="MIGRLFRIFFFFALAALIINRLFSRRQKRALCEVAKISAWVLLG
                     SAAATLFWYLVMLYFKHIPDSY"
     gene            438..1577
                     /locus_tag="WGQ68_001876"
     CDS             438..1577
                     /locus_tag="WGQ68_001876"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HEZ0715628.1"
                     /translation="MSQNASVGIVTPQKIPFEVPLVLENGKTLPRFDLMIETYGELNA
                     EKNNAVLICHALSGNHHVAGKHSAEDKYTGWWDNMVGPGKPIDTERFFVVGLNNLGGC
                     DGSSGPLSINPETGREYGADFPLVTVKDWVKSQAALADYLSIGQWAAVVGGSLGGMQA
                     LQWAISYPERVRHALVIASAPKLSTQNIAFNDVARQAILTDPDFNEGHYRSRNTVPAR
                     GLRIARMMGHITYLAEDGLGKKFGRDLRSNGYQYGYGVEFEVESYLRYQGDKFVGRFD
                     ANTYLLMTKALDYFDPAADFGDSLTRALQNVKAKFFVASFSTDWRFAPERSHELVKAL
                     IAAQKSVQYIEVKSAHGHDAFLMEDEAYMRAVAAYMNNVYKGCQQ"
     gene            1574..2155
                     /gene="metW"
                     /locus_tag="WGQ68_001877"
     CDS             1574..2155
                     /gene="metW"
                     /locus_tag="WGQ68_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine biosynthesis protein MetW"
                     /protein_id="HEZ0715629.1"
                     /translation="MNLRDDLQLIYDRIPEGSRVLDLGCGDGELLAALVEHKKCSGYG
                     IEIDTDSVIAAMSRGVNVIQADLEEGLTAFNDQSFDVIVLSQTIQAMQNTEKILRCLM
                     RVAKQAIVSFPNFGYWRNRVQIALGGHMPVSERMPYHWYDTPNIHWCTLKDFDLLCAK
                     NNIRVLERAVMTGNRQVKHFPNLLGSLAFYRVG"
     gene            2272..3117
                     /locus_tag="WGQ68_001878"
     CDS             2272..3117
                     /locus_tag="WGQ68_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="d-alanine-poly(phosphoribitol) ligase"
                     /protein_id="HEZ0715630.1"
                     /translation="MKNKTSSLLLWLAAIMLTACSPNKEDKTKETGASAASSPASAAS
                     SSAPQTDLQPAASAPDNVKQAESAPPSNCTDLHPATGIDDLMQQIAEHIDSDCLFALS
                     HHELETRFGLPDSGYDNIQRLLFPDIRPEDPDYHQKIILAIEDLRYGKRTISRQAQDA
                     LMEQERRLREATLLLTQGSEETRGQGEEPKRTRYFEVSATPAYSSRHNNGLGGNFQYI
                     GQLPGYLKIHGEMLENQSLFRLSNRERNPDKPFLDIHFDENGKITRIVVYEKNIYFNP
                     NLGRR"
     gene            complement(3166..3726)
                     /gene="efp"
                     /locus_tag="WGQ68_001879"
     CDS             complement(3166..3726)
                     /gene="efp"
                     /locus_tag="WGQ68_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEZ0715631.1"
                     /translation="MKTAQELRAGNVFMVGNDPMVVQKTEYIKGGRSSAKVSMKLKNL
                     LTGAASETIYKADDKFDVVILSRKNCTYSYFADPMYVFMDEEFNQYEIEADNIGDALK
                     FIVDGMEDQCEVTFYEGNPISVELPTIIVREVEYTEPAVKGDTSGKVMKTARLVGGTE
                     IQVMSYIENGDKVEIDTRTGEFRKRA"
     gene            complement(3769..4917)
                     /gene="earP"
                     /locus_tag="WGQ68_001880"
     CDS             complement(3769..4917)
                     /gene="earP"
                     /locus_tag="WGQ68_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P maturation arginine
                     rhamnosyltransferase EarP"
                     /protein_id="HEZ0715632.1"
                     /translation="MNTPPFVCWIFCKVIDNFGDIGVSLRLARVLHRELGWRVHLWTD
                     DVSALRALCPDLPDVPCVHQDIDVRTWHSDAADIDTAPVPDVVIETFACDLPENVLHI
                     IRRHKPLWLNWEYLSAEESNERLHLMPSPQEGVQKYFWFMGFSEKSGGLIRERDYCEA
                     VRFDSGALRKRLMLPEKNAPEWLLFGYRSDVWAKWLEMWRQAGSPMTLLLAGTQIIDS
                     LKQSGVIPQDALQNDGDVFQTASVRLVKIPFVPQQDFDKLLHLADCAVIRGEDSFVRA
                     QLAGKPFFWHIYPQDENVHLDKLHAFWDKAHGFYSPETASVHRRLSDDLNGGEALSAT
                     QRLECWQTLQQHQNGWRQGAEDWSRYLFGQPSASEKLAAFVSKHQKIR"
     gene            complement(4936..5214)
                     /locus_tag="WGQ68_001881"
     CDS             complement(4936..5214)
                     /locus_tag="WGQ68_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715633.1"
                     /translation="MSRNLLVRWLAVCLIPLATLAVFAANPPEDKLQHLINGIILACE
                     ATFLFKFVLFDTIKHHLKQEFDLKRQTMFLFIPIVLLVVYLFHYFGAF"
BASE COUNT         1363 a         1412 c         1445 g         1226 t
ORIGIN      
        1 cacctcctac cgcaagccct tttcatcaaa tatgccgtct gaaacagaaa aatgcgtttc
       61 aggcggcatt ttttccgtca atacgaatcc ggaatgtgtt tgaaatacag catgaccaaa
      121 taccaaaaca gtgtcgcggc ggctgagcca agcagtaccc atgcgctgat tttggcgact
      181 tcgcacaggg cgcgtttttg cctgcggctg aaaaggcggt tgataatcaa cgcggcaagt
      241 gcgaaaaaga aaaaaatacg gaaaagtctg cctatcatgt cgggaagctg ggttaggacc
      301 gtctgaaaaa gtatggttcg gttgcggcgg catgggaacg aatgtttcgg cgtgtcaggc
      361 tgcttgtccg accgtgtcaa aaaatcatgc cgtttgcgat ataataacgc ttttcgccgc
      421 atttgttgga acaggatatg agtcaaaatg cctcggtggg cattgtaacg ccccaaaaaa
      481 ttccgtttga ggtgccactg gttttggaaa acggtaaaac tttgccgcgt ttcgatctga
      541 tgattgaaac ctacggcgag ctgaatgctg aaaaaaacaa tgcggtttta atctgccacg
      601 cgctgtcggg caaccatcat gttgcgggca aacattcggc ggaggataaa tatacgggct
      661 ggtgggacaa tatggtcggt cccggaaaac cgattgatac ggaacgtttt ttcgtggtcg
      721 gtttgaataa tctgggcggc tgcgacggca gcagcgggcc tttgtcgatc aatcctgaaa
      781 cgggtaggga atacggcgcg gattttccgc tggttacggt gaaggactgg gtaaaatccc
      841 aagccgcgct tgccgattat ctcagcatcg ggcaatgggc ggcggttgtc ggcggcagct
      901 tgggcggcat gcaggctttg cagtgggcga tttcctatcc cgaacgcgtg cgccacgcct
      961 tggtgattgc gtccgcgccg aaactgtcca cgcaaaatat cgcgttcaac gatgtggcgc
     1021 gtcaggcgat tttgaccgat cccgatttca acgaaggaca ttaccgcagc cgcaacaccg
     1081 ttcccgctcg gggcttgcgg attgcccgca tgatggggca catcacctat cttgccgaag
     1141 acggtttggg caaaaaattc ggacgcgatt tgcgttccaa cggctatcaa tacggctatg
     1201 gcgttgaatt tgaagtagaa tcctatctgc gctatcaagg cgacaaattc gtcgggcggt
     1261 ttgatgccaa cacctatttg ctgatgacca aggctttgga ctatttcgat ccggcggcag
     1321 atttcggcga cagcctgacc cgcgccctgc aaaatgtgaa agcgaaattc tttgtcgcca
     1381 gcttcagcac cgactggcgt ttcgcacccg aacgttcgca cgaactggtc aaggcactga
     1441 ttgccgccca aaaatccgtg cagtatatcg aagttaaatc cgcacacggg cacgatgcct
     1501 ttttaatgga agacgaagcc tatatgcgtg cggtcgccgc ctatatgaac aacgtttata
     1561 agggatgtca gcaatgaacc tgcgcgatga tttgcaactg atttacgacc ggatacccga
     1621 aggcagccgc gtcttggact tgggctgcgg cgacggcgaa ctgcttgccg cattggtcga
     1681 acacaaaaaa tgcagcggct acggcatcga aatcgacacc gacagcgtga ttgccgcgat
     1741 gtcgcgcggc gtgaatgtta tccaagccga tttggaagaa ggtttaaccg cattcaacga
     1801 tcaaagtttt gatgtgattg tgttgagcca aaccatccaa gcgatgcaga ataccgaaaa
     1861 aatcctgcgc tgcctgatgc gcgttgccaa acaggcgatt gtcagcttcc cgaatttcgg
     1921 ctactggcgc aaccgtgtcc aaatcgcact cggcggacat atgccggttt ccgaacgtat
     1981 gccctaccat tggtacgaca cgcccaatat ccattggtgc acgctcaaag actttgattt
     2041 gttgtgcgcc aaaaacaaca tccgcgtcct cgaacgcgcc gtgatgacgg gcaacagaca
     2101 ggtcaaacat ttccccaacc ttttgggcag cctggcgttt taccgcgtcg ggtaagacag
     2161 gttgagaacc ttgtcggcaa taacaggttc ttcggttcta ttaacaacag gccgtctgaa
     2221 aaatgaaacg gcaggttttc agacggcctt tttaatattg gaaacaatag aatgaaaaac
     2281 aaaacctcat cacttctctt atggcttgcc gcaatcatgc tgaccgcgtg ttccccgaac
     2341 aaagaagata aaaccaaaga aaccggcgca tctgccgctt cgtccccggc atccgccgct
     2401 tcgtcttccg cgccccaaac cgatttgcaa ccggccgcat ccgcccctga taacgtcaag
     2461 caggcagaaa gcgcgccgcc gtcaaattgc accgacctgc accccgccac cggcattgac
     2521 gatctcatgc agcaaatcgc cgaacacatc gactcggact gtctgtttgc cctttcccat
     2581 cacgaactgg aaacccgttt cggcttaccc gacagtggct atgacaacat acagcggctg
     2641 ctgtttcccg acatccgccc tgaagatccc gactaccatc agaaaatcat actggcaatt
     2701 gaagacttgc gttacggaaa gcgcacgatc agccggcagg cacaagatgc cttgatggaa
     2761 caggaacgcc gcctccgaga agcgacgctg ttgctgacac agggcagcga agaaacccgc
     2821 gggcaaggcg aggagccgaa acgcacgcgt tattttgaag tttcggcaac ccctgcctat
     2881 tcgagccggc acaacaacgg acttggcggc aatttccaat acatcggcca attgcccggc
     2941 tatctgaaaa tacacggaga aatgcttgaa aaccaatcac tcttccggct gtccaaccgt
     3001 gaacgcaatc ccgacaaacc gtttttagac atccattttg acgaaaatgg caaaatcacg
     3061 cgtattgtcg tttacgaaaa aaacatctac ttcaatccaa acttggggcg aagataaaaa
     3121 aatgccgtct gaaagccttt cagacggcat ttttcaagta ggatatcagg cgcgtttgcg
     3181 gaattcgccg gtgcgggtat cgatttcgac tttgtcgccg ttttcgatgt aagacatcac
     3241 ttggatttcg gtgccgccga ccagacgcgc ggttttcatc actttgccgg aagtgtcgcc
     3301 tttgacagca ggctcggtgt actcgacttc gcgtacgatg atggtaggca gttctacgga
     3361 gatggggttg ccttcgtaga aggtaacttc gcattggtct tccataccgt caacgatgaa
     3421 tttcaacgcg tcgccgatgt tgtcggcttc gatttcgtat tggttgaatt cttcgtccat
     3481 aaagacgtac atcgggtcgg caaaatagct gtacgtacag tttttgcggg acaggatgac
     3541 cacgtcgaat ttgtcgtcgg ctttgtaaat ggtttcggaa gccgcgccgg tcagcaggtt
     3601 tttcagtttc atgctgactt tggcggaaga gcggccgcct ttgatgtatt cggttttttg
     3661 aacgaccata ggatcgttgc cgaccataaa tacattgccg gcgcgcagtt cttgtgctgt
     3721 tttcattgtg attttccaat caaatcggtt gtcgtaaaac gcgctattct agcgtatttt
     3781 ttgatgcttt gaaacaaagg cggcgagttt ttcggatgcg gaaggctgcc cgaaaagata
     3841 acggctccaa tcctccgcgc cttgccgcca gccgttttga tgttgttgca gggtttgcca
     3901 acattcgagg cgttgcgtgg cggataaagc ctctccgccg ttgaggtcgt ctgaaaggcg
     3961 gcggtgtacc gatgcggttt cgggcgagta aaatccatgc gccttatccc aaaaggcgtg
     4021 gagtttatcg agatggacat tctcgtcttg cgggtagatg tgccaaaaga agggtttgcc
     4081 ggcaagctgg gcgcgcacga aactgtcttc gccgcgtatc acggcgcagt cggcgaggtg
     4141 cagcagtttg tcgaagtcct gttgcggcac gaaagggatt ttgacgaggc ggacggatgc
     4201 cgtctgaaaa acatcgccgt cgttttgcag ggcatcttgc ggaataacgc cgctttgttt
     4261 gaggctgtcg atgatttgcg tccccgccag caacagtgtc atcgggctgc ctgcctgtcg
     4321 ccacatttcc agccactttg cccaaacatc gctccgatag ccgaaaagca gccattcggg
     4381 ggcgtttttt tcgggaagca tcagcctctt gcgcaaggct ccgctatcga aacggacggc
     4441 ttcgcagtaa tcgcgttcgc gtatcagtcc gccgcttttt tcgctgaagc ccataaacca
     4501 aaaatatttt tgaacacctt cctgcggcga aggcatcagg tgcagccttt cattgctttc
     4561 ctccgcgctc aaatattccc aattcagcca aagcggcttg tgtcggcgga taatgtgcag
     4621 cacattttcg ggcaggtcgc aggcaaaagt ttcgatgacg acatcgggaa caggcgcggt
     4681 atcaatgtct gccgcatcgg aatgccaagt gcggacatca atatcctgat gaacacaggg
     4741 aacatcgggc aaatcagggc aaagcgcacg caaggcggac acatcgtccg tccacaaatg
     4801 cacccgccaa ccgagttcgc gatgcaaaac acgggcaagc cgcagggaaa cgccgatatc
     4861 gccgaaattg tcgatgacct tgcaaaaaat ccagcagaca aaaggaggag tattcatagg
     4921 aataacggaa acgggctaaa acgcgccgaa atagtggaac agatacacaa ccagcaaaac
     4981 aatcggaata aacagaaaca tagtttgacg tttcaaatca aactcttgct tcaaatgatg
     5041 cttgatggtg tcgaaaagga cgaatttgaa caaaaacgtc gcttcgcagg caaggatgat
     5101 gccgttgatc agatgctgga gtttgtcttc gggcggattg gcggcgaaaa cggcaagcgt
     5161 cgccaacggg atgaggcaga cggcaagcca acggacaagc aggtttcgag acattgtaag
     5221 gctttcaaaa aaagcaaaac gccattatcg ccccaagcgc acgccttgtc taacggacgc
     5281 accggagatg ccgtctgaag ccttgccgca ggaaaacacc gcaccgccga acctttaaat
     5341 acgcccgaga cgggcgttcc ccgaaaaatc ggacgctgcc gccgatgcct gaggacaagg
     5401 cggcagcagg cacgggcata ttgaaaaccc ggcgcgtcgg tttgaa
//
LOCUS       DAWFHY010000109         5373 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.109, whole genome shotgun sequence.
ACCESSION   DAWFHY010000109 DAWFHY010000000
VERSION     DAWFHY010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.109"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..792
                     /locus_tag="WGQ68_001882"
     CDS             <1..792
                     /locus_tag="WGQ68_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ0715634.1"
                     /translation="DGAIGAAVGEIVGEALVKNTDFSDMTPEQLDLEVKKITAYAKLA
                     AGTVAGVTGGDVNTAAQTAQNAVENNAVKAVVTAAKVVYKVARKGLKNGKINVRDLKQ
                     TLKDEGYNLADNLTTLFDETLDWNDAKAVIDIVVGTELNRANKEEAAQKVKEVLEKNR
                     PYIPNKGAVPNMSTYMKNNPFGKQLAQISEKTTLPTQQGQSVFLVKRNQGLLKTGDRF
                     YLDGQHKNHLEVFDKNGNFKFVLNMDGSLNQMKTGAAKGRKLNLK"
     gene            801..1178
                     /locus_tag="WGQ68_001883"
     CDS             801..1178
                     /locus_tag="WGQ68_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715635.1"
                     /translation="METLNDIKKILINVGLYQGFDLTDPKVSEEVNHETANMKWIKDY
                     TSDGNWDNEFKEDLKNFLDYMEVCQLALNDKNFKIASNSLFMAMIYAGNLSLIFDSIK
                     TDISTLLSAEYKKNSFSWPSLDE"
     gene            1456..1830
                     /locus_tag="WGQ68_001884"
     CDS             1456..1830
                     /locus_tag="WGQ68_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715636.1"
                     /translation="MKNFNVVKESLRELGIKQGFDLYEKATTEKLNSEDPLDLQWLSN
                     YSSDWNDELEEDFDSFFQHMKEYQYAIDNEDIKSACSSLCEAMLYVGNIKNFFEFLKS
                     DMIRLLRGESKTTDFQWPQFDE"
     gene            1865..3307
                     /locus_tag="WGQ68_001885"
     CDS             1865..3307
                     /locus_tag="WGQ68_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0715637.1"
                     /translation="MRKVCASARTRSMIYGSMPSEKLTIFQTAFVMQVNIQIPYILPR
                     CVRAEDTPYACYLKQLQVTKDVNWNQVQLAYDKWDYKQEGLTGAGAAIIALAVTVVTA
                     GAGAGAALGLNGAAAAATDAAFASLASQASVSLINNKGNIGNTLKELGRSSTVKNLMV
                     AVATAGVADKIGASALNNVSDKQWINNLTVNLANAGSAALINTAVNGGSLKDNLEANI
                     LAALVNTAHGEAASKIKQLDQHYITHKIAHAIAGCAAAAANKGKCQDGAIGAAVGEIV
                     GEALVKNTDFSRMSATEIEKAKAKITAYSKLVAGTASAVVGGDVNTAANAATVAVENN
                     SLFQPQTTLEAGVRNAILRGDIQELRLLLGEANFSTADAAYAQRILASMEKIGESNSR
                     LLAERYGVDWLNKVHHIFKGHQGSIGNTLIQKSGSMENAVVATQKAVDALKLTKTGNY
                     PVTVTVNGITVIVRVYVNNGVSRIATILKM"
     gene            4345..4852
                     /locus_tag="WGQ68_001886"
                     /pseudo
     CDS             4345..4852
                     /locus_tag="WGQ68_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675736.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
     gene            4857..5240
                     /locus_tag="WGQ68_001887"
     CDS             4857..5240
                     /locus_tag="WGQ68_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003675734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715638.1"
                     /translation="MEKIKNYIEFKKLFKNITEKFVRDLDITLIEAAEYSIIEFEIYL
                     SEYPEDSTFIFTAWAIYLLENNCLNERGKDNFFIAEMKKCYQQTNLDRIKSIYSSSDY
                     HIFAKDVNKIQQTFKLNLANIMQQE"
BASE COUNT         1816 a          917 c         1154 g         1486 t
ORIGIN      
        1 gatggtgcga tcggtgcggc tgtgggtgag attgtcgggg aggctttggt taaaaatacc
       61 gattttagcg atatgacccc ggaacaatta gatctggaag ttaagaaaat taccgcctat
      121 gccaaacttg cggcaggtac agttgcaggc gtaacgggag gagatgtcaa tactgctgca
      181 caaaccgcac aaaacgcggt agaaaataat gcggttaaag ctgttgtaac tgctgcaaaa
      241 gtggtttata aggtagccag aaaaggatta aaaaacggga aaatcaacgt tagagattta
      301 aaacagacgt tgaaagacga aggttataat ttagccgaca acctgaccac cttattcgac
      361 gaaacattgg attggaacga tgccaaagcc gttattgata ttgtcgtcgg aacagagctg
      421 aatcgcgcta ataaagagga agcggcacaa aaggtcaagg aagttttaga aaaaaatcgt
      481 ccttatatcc ctaataaagg tgctgtaccg aatatgagta catacatgaa aaataatcct
      541 tttggaaaac agctggctca aatttcagaa aagacaacgc ttccgacgca gcaagggcag
      601 tctgtcttct tggtaaaaag aaaccaaggg ttattaaaaa ccggtgatag gttttattta
      661 gatggccaac ataaaaatca tttagaggtt tttgataaaa atgggaactt taagtttgtt
      721 ctaaatatgg atggttcgct taaccaaatg aaaactgggg cagcaaaagg tcgtaaatta
      781 aacttaaaat aggaaatttt atggaaacat tgaatgatat aaaaaaaatc ttgattaatg
      841 tggggcttta tcaagggttt gatttgacag atccaaaagt atcagaagaa gttaatcatg
      901 aaacagctaa tatgaaatgg attaaagatt atacttcaga cgggaattgg gataatgaat
      961 ttaaggagga tttaaaaaac tttttagatt atatggaagt atgccaatta gccctaaacg
     1021 ataaaaattt caaaattgcc agtaattctt tatttatggc tatgatttac gcaggtaatc
     1081 tatctcttat atttgattca ataaaaactg atatatcaac attattgagt gctgagtata
     1141 aaaagaatag tttttcatgg ccatctcttg atgaatagaa agcaagttgt agcctgcatg
     1201 aaatctaaaa cccatgcata aggtgtgggc ttcagtatac gcgttccatg atttacggcc
     1261 atatgccgtc tgaaaagctc aattttttca gacggcattt gttatgaaag taaatattta
     1321 gattccctgt atactgttta gactcgtgtg tgctgagtaa gctgtagtct gcatgaaacc
     1381 taaaactcgc tcaaaattaa gctaagacat tagcagggca agggcgaaaa ttgaatctta
     1441 aataaggtga ttcagatgaa aaattttaat gtagtaaaag aaagtttaag agagttagga
     1501 attaaacaag gatttgatct ttatgagaaa gccacaactg aaaaattgaa tagtgaagat
     1561 cctcttgact tacaatggct ttctaactat tcatctgatt ggaatgatga attagaagaa
     1621 gactttgatt ctttttttca gcatatgaag gaatatcaat atgctattga caatgaagac
     1681 attaaatctg catgtagttc actatgtgaa gctatgctct atgttggtaa tattaaaaat
     1741 ttttttgagt ttctcaaaag cgatatgatt agactgttga gaggtgaaag taaaacaaca
     1801 gactttcaat ggccgcaatt tgatgaatag cagcaagctg tagcctgcat gaaacctaaa
     1861 atccatgcgt aaggtgtgtg cttcagcacg cacgcgttcc atgatttacg gctcaatgcc
     1921 gtctgaaaag ctcacaattt ttcagacggc atttgttatg caagtaaata ttcagattcc
     1981 ctatatactg cccagatgcg tgcgtgctga agacaccccc tacgcttgct atttgaaaca
     2041 gctccaagtc accaaagacg tcaactggaa ccaggtacaa ctggcgtacg acaaatggga
     2101 ctataaacag gaaggcttaa ccggagccgg agcagcgatt attgcgctgg ctgttaccgt
     2161 ggttactgcg ggcgcgggag ccggagccgc actgggctta aacggcgcgg ccgcagcggc
     2221 aaccgatgcc gcattcgcct cgctggccag ccaggcttcc gtatcgctca tcaacaacaa
     2281 aggcaatatc ggtaacaccc tgaaagagct gggcagaagc agcacggtga aaaatctgat
     2341 ggttgccgtc gctaccgcag gcgtagccga caaaatcggt gcttcggcac tgaacaatgt
     2401 cagcgataag cagtggatca acaacctgac cgtcaacctg gccaatgcgg gcagtgccgc
     2461 actgattaat accgctgtca acggcggcag cctgaaagac aatctggaag cgaatatcct
     2521 tgcggctttg gtgaatactg cgcatggaga ggcagcaagt aaaatcaaac agttggatca
     2581 gcactacatt acccacaaga ttgcccatgc catagcgggc tgtgcggcag cggcggcgaa
     2641 taagggcaag tgtcaagatg gcgcgatagg cgctgcagtc ggtgagattg ttggtgaggc
     2701 tttggttaag aatactgatt tcagtcgtat gagtgcgacc gaaatcgaaa aagctaaagc
     2761 gaagattact gcctattcaa aactggttgc cggcactgcg tctgccgttg taggcgggga
     2821 tgtgaataca gcggcgaatg cggctactgt ggcggtagag aataattccc tctttcagcc
     2881 acaaaccaca ttagaagcgg gagtaagaaa tgcaatttta cgaggagata ttcaggaatt
     2941 acggcttttg ttgggcgaag caaatttttc aacagctgat gctgcctatg ctcaaaggat
     3001 tttagcttcg atggaaaaaa taggtgaaag taattctcga ctattggctg aaagatacgg
     3061 tgtagattgg ctaaataaag ttcatcacat ctttaagggg catcaaggaa gcatcggtaa
     3121 tacattgatt caaaaatcag gctccatgga aaatgctgtt gtagcgacgc aaaaggcagt
     3181 ggatgcttta aaactgacaa aaacaggaaa ttatccagtt actgtaacgg tcaatggtat
     3241 aacggtaatt gttcgcgtat atgtcaataa tggagtatct cgtattgcaa ctattttgaa
     3301 aatgtaaggg tagaatatat gaaccagttt tgtttatctg aatatgagaa ggctttttta
     3361 tgtagaatta ttgaaaacaa tatgatatgg aaggaatatt taatagagca aatagaaaat
     3421 tcaataatag tatctagaga taatacagga tgtgggtgct acattgattt tcaggtaaat
     3481 ttagaaagaa ctaatctaaa taaatacgaa aatatcccta tttttttgaa ggagacatca
     3541 ctgcaccgga taagtcagat gcagcgactt tcctgctata tactacagag tctttgaata
     3601 aagcaggtag aattattgac tttctggaag ttgctattgc aggcacaaat tatgatgaag
     3661 ttgaggtcta ttcatggtta aagatggaaa atagcctacg gttcggctta taaaataaac
     3721 tgactgaaaa tctgagatat tttcaataat ataataaatt attaaaattt tatgctcaat
     3781 cccattttta attagtcaag cgaaaatttt ttagcgtatt tttatgcgtc aaatttcgtt
     3841 aacatactat tgaagttttt ctttatattg ccgcattatc agttttacca atgatacgct
     3901 gtatctggct agtgtggcgc gatctgtcaa ctagggcaga ctgattgtgc tggtacgaaa
     3961 atcacccaag gacaatacgg ttgatttttg aagaaacaat atacataggt aacatatttt
     4021 attaaccaag cctctggagg taacacagct tgggcagctt cgatactgaa aacgccccag
     4081 tcaatgggta atctcactat tccttccaaa gatattaata acactttatc gaaagcctat
     4141 caaacattga gtcgttatga ttcttttgat tacaaatcag ctgttgccgc acaacctgca
     4201 ctttacttat taaacggacc gcttggcttc agtgtcaaag cagctactgt ggcagcaggg
     4261 ggatataaca ttggacaggg agcgaaagca atctctaatg gagaatatct gcatggtaca
     4321 gttcaggttg ttaatggcac attgatggtt gcaggatctg tatctgcaca ggctgcaata
     4381 tcggccaagc ctacacctgt tacccgttat ctgagcaatg acagtgctcc tgcttttaac
     4441 tgctgaaagc cagagagtcc gcatgaaact gccggaagag tatcgacaaa tagggaatct
     4501 tgcgatagca aaaattgatg ttaaaggatt accgcaaagg atggaagcat ttagttcttt
     4561 ccaaaaaggg gaacatggat ttatttcgtt acctgaaaca aaaattttta aacctatatc
     4621 tgttgataaa tatcataata ttgcctctcc tcctagagga acattaagaa atatagatgg
     4681 agaatataaa ttacttgaaa ctatagcaca gcaactcgga aataatcgta atgtatcagg
     4741 tagaattgat ctatttacag aattaaaggc ctgtcaatct tgcagcaatg ttattttaga
     4801 gtttagaaat cgctatccaa atattcaatt aaatattttt acaggaaaat agaatcatgg
     4861 aaaaaataaa aaattatata gaattcaaga aattatttaa aaatataacg gaaaaatttg
     4921 tcagggattt agatataaca ttaattgaag cagctgaata ttctataatt gaattcgaaa
     4981 tttatttatc tgaatatccg gaagatagta catttatttt tacagcatgg gcaatctatc
     5041 tgttagaaaa taactgtctc aatgaaagag gaaaagataa ttttttcatc gcggaaatga
     5101 aaaaatgtta tcaacaaact aatttggata gaataaaatc tatctattct tcgagtgact
     5161 atcatatttt tgctaaggat gtaaacaaga ttcaacaaac atttaaatta aatcttgcca
     5221 atatcatgca gcaagagtag caactgtatt tttaaccccg tcgggcaaaa ataccaaaaa
     5281 cagtaaatca ctaaaaatac ttttataccg cagcagcaag ctgtagcctg catgaaacct
     5341 aaaatccatg cgtaaggtgt gtgctccagc acg
//
LOCUS       DAWFHY010000110         5215 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.110, whole genome shotgun sequence.
ACCESSION   DAWFHY010000110 DAWFHY010000000
VERSION     DAWFHY010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5215
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.110"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(1..381)
                     /locus_tag="WGQ68_001888"
                     /pseudo
     CDS             complement(1..381)
                     /locus_tag="WGQ68_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor"
     gene            complement(467..682)
                     /locus_tag="WGQ68_001889"
     CDS             complement(467..682)
                     /locus_tag="WGQ68_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715639.1"
                     /translation="MAGATIRIIDRQTDEVIAKKTIYVFEKGLDGTGGARMPWKFAIL
                     CNKERLTSSEPLSDFVLSVLKPYILRP"
     gene            complement(683..979)
                     /locus_tag="WGQ68_001890"
     CDS             complement(683..979)
                     /locus_tag="WGQ68_001890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715640.1"
                     /translation="MLLKVVPERTVSADAKTRDPMWDNAALQTSEGVNFIARFLGFFS
                     DGEYRYVDVLQPNHSDIIRYSGKDFPLNQILNHIHPARYAVTFENNVDSKLRRH"
     gene            complement(970..1312)
                     /locus_tag="WGQ68_001891"
                     /pseudo
     CDS             complement(970..1312)
                     /locus_tag="WGQ68_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218854.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1548..2975
                     /locus_tag="WGQ68_001892"
     CDS             1548..2975
                     /locus_tag="WGQ68_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEZ0715641.1"
                     /translation="MFFSALKSFLSRYITVWRNVWAVRDQLEPPKRTAEEQAFLPAHL
                     ELTDTPVSAAPKWAARFIMAFALLALLWSWFGKIDIVAAASGKTVSGGRSKTIQPLET
                     VVVKAVHVRDGQHVKQGETLAELEAVGTDSDVVQSEQALQAAQLSKLRYEAVLAALES
                     RTVPHIDMAQARSLGLSDADVQSAQVLAQHQYQAWAAQDAQLQSALRGHQAELQSAKA
                     QEQKLVSVGAIEQQKTADYRRLRADNFISEHAFLEQQSKSVSNWNDLESTRGQMRQIQ
                     AAIAQAEQNRVLNTQNLKRDTLDALRQANEQIDQYRGQTDKAKQRQQLMTIQSPADGT
                     VQELATYTVGGVVQAAQKMMVVAPDDDKMDVEVLVLNKDIGFVEQGQDAVVKIESFPY
                     TRYGYLTGKVKSVSHDAVSHEQLGLVYTAVVSLDKHTLNIDGKAVNLTAGMNVTAEIK
                     TGKRRVLDYLLSPLQTKLDESFRER"
     gene            3044..4447
                     /locus_tag="WGQ68_001893"
     CDS             3044..4447
                     /locus_tag="WGQ68_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEZ0715642.1"
                     /translation="MTLLNLMIMQDYGISVCLTLTPYLQHELFSAMKSYFSKYILPVS
                     LFTLPLSLSPSVSAFTLPEAWRAAQQHSADFQASHYQRDAVRARQQQAKAAFLPHVSA
                     NASYQRQPPSISSTRETQGWSVQVGQTLFDAAKFAQYRQSRFDTQAAEQRFDAAREEL
                     LLKVAESYFNVLLSRDTVAAHAAEKEAYAQQVRQAQALFNKGAATALDIHEAKAGYDN
                     ALAQEIAVLAEKQTYENQLNDYTGLDSKQIEAIDTANLLARYLPKLERYSLDEWQRIA
                     LSNNHEYRMQQLALQSSGQALRAAQNSRYPTVSAHVGYQNNLYTSSAQNNDYHYRGKG
                     MSVGVQLNLPLYTGGELSGKIHEAEAQYGAAEAQLTATERHIKLAVRQAYTESGAARY
                     QIMAQERVLESSRLKLKSTETGQQYGIRNRLEVIRARQEVAQAEQKLAQARYKFMLAY
                     LRLVKESGLGLETVFAE"
     gene            complement(4611..5204)
                     /locus_tag="WGQ68_001894"
     CDS             complement(4611..5204)
                     /locus_tag="WGQ68_001894"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HEZ0715643.1"
                     /translation="MYGSHRLLCTQTDGDNAQKKTTTYKEQDPAKVTHYLTQPAEFSD
                     YQRVYLDETGFDRYLFRPYARSLKGQIVKAQISGKRYQRLSLVSAQVGNRLIAPMVYQ
                     NTMTGVFFEAWFQQCLLPALTQKSVIILDNARFHRMGVLREMAEKLGHKVLPLAPYSP
                     ELNPIEKVWANIKRYLRTVLSDYARFDDALLSYFDFN"
BASE COUNT         1329 a         1275 c         1343 g         1268 t
ORIGIN      
        1 ttgtagatac cgaaaccgaa aacttcttcg gcgagcatgg acgcggcgcg aataaaatct
       61 tcgtcttcca aaagacttac acgaacgccg tatttatcga aaaaggtttt ttcatgaaat
      121 gaaaagctaa tttgatcaat gaaagccgaa tctgatacac cgcgccgaag aggaacgcct
      181 aacaggtttc ctttaccgtc cgttatgtac gtttcgtaac attcgaagac ttcctgaacc
      241 ctgcctgccg tttcggtttc tgtacccccc cctgttagat aaggggggaa ggtttgaagc
      301 ggttgccggc ttcctgccgt ccgctggcgc gtccgtcatc acgccgacaa ccgcctttgt
      361 catcccttgc ttatcttcca tggtgcgaat cctcaaaaac gggcaaaaaa aaagccctgt
      421 tacttgtaga aagtaaagga cgttaatttt tgttaatcgt cccttcttag ggacgcaata
      481 tataaggttt taaaacgcta agaacaaaat ccgataacgg ctctgaagaa gtaagtcttt
      541 ctttattgca caagatagca aacttccacg gcattctcgc cccacccgtg ccgtccaagc
      601 ctttttcaaa gacatagatg gttttcttgg caatcacttc gtcagtttgg cggtcgataa
      661 tccgtatggt cgcacctgcc actcagtgcc tgcgcagctt ggaatcgaca ttgttttcga
      721 acgttaccgc ataacgggcg gggtgtatat ggttaagtat ttgatttagc ggaaaatctt
      781 tacctgaata ccgaataata tcggaatggt tgggttgcag gacatccaca tagcggtatt
      841 ccccatcgct aaaaaatcct aggaaacgag caataaaatt tacgccttcg ctggtctgta
      901 aagccgcatt gtcccacatc gggtctctgg tttttgcatc tgccgaaacg gtacgctcag
      961 gtactacctt caacagcata atcccttcca cattgtccgc cgtctggtaa atctttcccc
     1021 cgccgttttg cattgttcgt taaacacggc ttcggcttct ttgtattttc tgtcccactc
     1081 ttcttgtgcc tgtatttctt ctttgctcgg gcagaattgt ttgggaataa tccaaacaaa
     1141 cagcatcagg atagcggcgg cggtcaggct gcctgaaagg attttgccgg ggttttttgt
     1201 aggcaaagcg gacgagaaac caaagcaaca gcagcatggt gtcccaatag ccgattgaga
     1261 ataggatggc caaaccttct aggaaatggc gtaaatcgtt tgtggtaaac atgggtagtt
     1321 cctgtggtta aatgtgcagg ctgctttttg ccgaaccttg ccgcatctca aaagcagcct
     1381 gcgcttcagc gttgcgttac gcagtaaaat aatgaatatt tgtaacgact tgggtatttt
     1441 ttgtcaatat tcccgccttt cccttaacag ctgccgcgct ttccgttaaa attcctttac
     1501 atatttatat tgtttcctgt ttctatattg ccaaggttat acccgttatg tttttctccg
     1561 ccctgaaatc ctttctttcc cgctacatta ccgtatggcg caatgtttgg gcggtgcgcg
     1621 accagttgga accgcccaaa cgcacggcgg aagaacaggc gtttttgccc gcgcatttgg
     1681 aactgaccga tacgccggtc tctgccgctc cgaaatgggc ggcgcgtttt attatggcgt
     1741 ttgcgctttt ggctttgttg tggtcctggt tcggcaaaat cgatattgtg gcggcggctt
     1801 cgggcaaaac ggtgtcgggc gggcgcagca aaaccatcca gccgctggaa acggtggtgg
     1861 ttaaggcggt acatgtgcgc gacgggcagc atgtgaaaca gggagaaacg ctggcggaac
     1921 tggaggctgt gggaacagac agcgatgtgg tgcagtcgga gcaggctttg caggctgccc
     1981 aattgtccaa actgcgttat gaagcggtat tggcggcatt ggaaagccgt accgtgccgc
     2041 atatcgatat ggcgcaagca cggtctttag gtctctccga tgccgatgtg caatcggcgc
     2101 aggtgttggc gcagcaccag tatcaggcat gggcggcgca ggatgcgcaa ttgcagtcgg
     2161 ctttgcgcgg ccatcaggcg gaattgcagt cggccaaggc gcaggagcag aagctggttt
     2221 cggtgggggc gatcgagcag cagaaaacag cagactaccg ccgtttgcgg gccgacaatt
     2281 ttatttcgga acatgcgttt ttggagcagc agagcaaatc ggtcagcaat tggaacgatt
     2341 tggaaagtac gcgcggtcag atgaggcaga ttcaggcggc cattgcacag gcggagcaga
     2401 atcgggtgct gaatacgcag aacctgaaac gcgatacgct ggatgcgctg cgccaggcaa
     2461 acgaacagat tgaccaatac cgcggccaaa cggataaggc aaagcagcgg cagcagctga
     2521 tgacaataca gtcgcctgcg gacggcacgg tgcaggaatt ggccacctat acggtgggcg
     2581 gtgtggtgca ggctgcccaa aaaatgatgg tggttgcgcc cgatgacgac aaaatggacg
     2641 tggaagtttt ggtattgaac aaagacatcg gttttgtgga acagggacag gatgcggtgg
     2701 tgaagattga gagttttccc tatacgcgct acggttatct gacgggcaag gtgaaaagtg
     2761 tcagccatga tgcggtaagc cacgaacagt tgggcttggt ttatacggcg gtggtgtcgc
     2821 tggacaaaca taccttgaat attgacggca aagcagtgaa tctgacggcg ggcatgaatg
     2881 tcacggcgga gattaaaacg ggtaaacggc gggtgctgga ttatctgtta agcccgctgc
     2941 aaaccaaatt ggacgaaagc tttagggagc gataggcgga tccatactgg gcatttgtta
     3001 tccgccggtt cggacatgca gactgcctga aaccattgcc cggatgacat tgctcaatct
     3061 aatgataatg caagattacg gtatttccgt ttgcctgaca ctgacgccct atttgcaaca
     3121 tgaactattt tcggctatga aatcctattt ttccaaatat atcctacccg tttcactttt
     3181 taccttgcca ctatcccttt ccccatccgt ttcggctttt acgctgcctg aagcatggcg
     3241 ggcggcgcag caacattcgg ctgattttca agcgtcccat taccagcgtg atgcagtgcg
     3301 cgcacggcaa caacaagcca aggccgcatt ccttccccat gtatccgcca atgccagcta
     3361 ccagcgccag ccgccatcga tttcttccac ccgcgaaaca cagggatgga gcgtgcaggt
     3421 gggacaaacc ttatttgacg ctgccaaatt tgcacaatac cgccaaagca ggttcgatac
     3481 gcaggctgca gaacagcgtt tcgatgcggc acgcgaagaa ttgctgttga aagttgccga
     3541 aagttatttc aacgttttac tcagccgaga caccgttgcc gcccatgcgg cggaaaaaga
     3601 ggcttatgcc cagcaggtaa ggcaggcgca ggctttattc aataaaggtg ctgccaccgc
     3661 gctggatatt cacgaagcca aagccggtta cgacaatgcc ctggcccaag aaatcgccgt
     3721 attggctgag aaacaaacct atgaaaacca gttgaacgac tacaccggcc tggacagcaa
     3781 acaaatcgag gccatagata ccgccaacct gttggcacgc tatctgccca agctggaacg
     3841 ttacagtctg gatgaatggc agcgcattgc cttatccaac aatcatgaat accggatgca
     3901 gcagcttgcc ctgcaaagca gcggacaggc gcttcgggca gcacagaaca gccgctatcc
     3961 caccgtttct gcccatgtcg gctatcagaa taacctctac acttcatctg cgcagaataa
     4021 tgactaccac tatcggggca aagggatgag cgtcggcgta cagttgaatt tgccgcttta
     4081 tactggcgga gaattgtcgg gcaaaatcca tgaagccgaa gcgcaatacg gggccgccga
     4141 agcacagctg accgcaaccg agcggcacat caaactcgcc gtacgccagg cttataccga
     4201 aagcggtgcg gcgcgttacc aaatcatggc gcaagaacgg gttttggaaa gcagccgttt
     4261 gaaactgaaa tcgaccgaaa ccggccaaca atacggcatc cgcaaccggc tggaagtaat
     4321 acgggcgcgg caggaagtcg cccaagcaga acagaaactg gctcaagcac ggtataaatt
     4381 catgctggct tatttgcgct tggtgaaaga gagcgggtta gggttggaaa cggtatttgc
     4441 ggaataaagc aggctgaaac ggttatgaaa ttcccaaagc agcctgcacc ccgtttcgaa
     4501 agtgcaggct gctttgggat tgatccgata ctttcacatt ctcattgcct ttaaataaag
     4561 cacagttaaa aacacattta aattaagtgc gaattaattc aaatgtatat tcaattaaaa
     4621 tcaaaatagg acagtagtgc atcgtcaaat cgggcgtaat cagacaaaac ggttcgcaga
     4681 taccgcttaa tattcgccca cactttctca atcgggttga gctcaggtga ataaggtgca
     4741 agaggcaata ccttatgtcc caatttttcc gccatttccc gtaagacacc catacggtga
     4801 aatcgcgcat tatctaaaat aatcaccgat ttttgagtca atgcgggcag taggcattgc
     4861 tgaaaccacg cttcaaaaaa gactccggtc atcgtatttt gataaaccat cggagcaatc
     4921 agccggttgc cgacttgtgc ggacaccaaa gataagcgtt ggtatctttt tccacttatc
     4981 tgcgctttca ctatttgccc tttcaggctg cgggcatagg gacggaacag gtagcggtca
     5041 aatcctgttt catccaaata aacacgttgg tagtcggaaa attcggccgg ctgtgtcaaa
     5101 taatgcgtta ctttggccgg gtcttgttct ttgtaagtgg tggtcttttt ttgcgcgtta
     5161 tccccatctg tttgagtgca tagcaaacgg tggctgccgt acaatcaaaa tgttt
//
LOCUS       DAWFHY010000111         5083 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.111, whole genome shotgun sequence.
ACCESSION   DAWFHY010000111 DAWFHY010000000
VERSION     DAWFHY010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 5083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5083
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.111"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            1..88
                     /locus_tag="WGQ68_001895"
                     /pseudo
     CDS             1..88
                     /locus_tag="WGQ68_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245746.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            complement(335..1273)
                     /locus_tag="WGQ68_001896"
     CDS             complement(335..1273)
                     /locus_tag="WGQ68_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003753550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEZ0715644.1"
                     /translation="MLELNGLCKRFGGKTVADNICLTVGRGKILAVLGRSGCGKSTLL
                     NMIAGIVRPDGGEIQLNGENITRMPPEKRRISLMFQDYALFPHMSALENAAFGLKMQK
                     MPKAEAESLALLALAEVGLENEAHRKPEKLSGGEKQRLALARALVVRPSLLLLDESFS
                     SLDTHLRDRLRRMTAERIRNGGIPAVLVTHSPEEACTAADEIAVMHEGKILQCGTPET
                     LVQTPAGVQVARLMGLPNTDDDRHIPQNAVRFDQDGMECRVLSRTCLPESFSLSVLHP
                     EHGILWLNLDMPHAGEISGNDTVRIHIEDREIVRFR"
     gene            complement(1282..2157)
                     /locus_tag="WGQ68_001897"
     CDS             complement(1282..2157)
                     /locus_tag="WGQ68_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEZ0715645.1"
                     /translation="MPTVRFTESVSKHDLDALFEWAKASYGAESCWKTLYLNRLPLGN
                     LSPEWAERVKKDWEAGCSESSDGIFLNADGWSDMGGRLQHLARTWNKAGLLHGWRNEC
                     FDLTDGGGNPLFTLERAAFRPFGLLSRAVHLNGLTESDGRWHFWIGKRSPHKAVDPDK
                     LDNIAAGGVSGGEMPSEAVCRESSEEAGLDKTLFPFIRPVSRLHSLRPVSRGVHNEIL
                     YVFDAVLPETFLPENQDGEVACFEKMDIGSLVATMLSGNMMHDAQLVTLDAFCRYGLI
                     DAAHPLSEWLDGIRL"
     gene            complement(2339..2415)
                     /locus_tag="WGQ68_001898"
     tRNA            complement(2339..2415)
                     /locus_tag="WGQ68_001898"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2379..2381),aa:Met,seq:cat)
     regulatory      2565..2664
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2757..4658
                     /gene="thiC"
                     /locus_tag="WGQ68_001899"
     CDS             2757..4658
                     /gene="thiC"
                     /locus_tag="WGQ68_001899"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003686973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HEZ0715646.1"
                     /translation="MTTPKKTAKTSGNEARELADLSEDIGIRFKYPNSERVYLQGSRD
                     DIRVPLREIRQDDTYTAQGTEANPPIPVYDTSGVYGDPAAHIDLKQGLPHIRTAWLDE
                     RGDTEILPKLSSEYGIERAHDPKTAHLRFNQITRPRRAKAGRNVTQLHYARQGIITPE
                     MEFVAIRERLKLDELSQKPEYAKLLKQHAGQSFGANIPTHPDQITPEFVRREIAAGRA
                     IIPANINHPELEPMIIGRNFRVKINGNLGNSAVTSSLTEEVEKMVWSLRWGADTIMDL
                     STGAHIHETREWIIRNAPVPIGTVPIYQALEKTGGIAEDLTWDLFRDTLIEQAEQGVD
                     YFTIHAGVLLRYVPMTANRLTGIVSRGGSIMAKWCLAHHRENFLYTHFDEICEIMKAY
                     DVSFSLGDGLRPGCIADANDESQFAELHTLGELTSKAWKHDVQVMIEGPGHVPLQRVK
                     ENMTEELQHCFEAPFYTLGPLVTDIAPGYDHITSGIGAANIGWYGTAMLCYVTPKEHL
                     GLPDKEDVRTGIITYKLAAHAADLAKGWPGAQLRDNALSKARFEFRWRDQFRLSLDPE
                     RAESFHDETLPAEGAKIAHFCSMCGPKFCSMKITQEVRDYADKQKAQRQGMEEKAVEF
                     VKKGAKIYS"
BASE COUNT         1251 a         1403 c         1333 g         1096 t
ORIGIN      
        1 aaaaacagaa aaccaaaatc agaaacctaa aatccgtcat tcccgcgcag gcgggaatct
       61 aggtctgtcg gcacggaaac ttatcgggaa aaacggtttc tttagatttt atgttctaga
      121 ttcccgcttt tgcgggaatg acgatgaaaa gatttttgtt gtttcggata aatttttgcc
      181 gtgttgggtt ctagattccc gcttttgcgg gaatgacggc agggtggttt ctgttgtttc
      241 ggataaattt tgcagccctg ataaaaaata tggctgcttt ggtaaaaaat gccgtctgaa
      301 aggttttcag acggcatttt gtttttaaga agcatcagcg gaagcggacg atttccctgt
      361 cttcgatatg gatgcgtacc gtatcgtttc ccgatatttc accggcgtgc ggcatatcga
      421 ggttcagcca caggatgccg tgttccggat ggaggacgga caggctgaac gattcgggca
      481 aacaggtacg ggataatacg cggcactcca tgccgtcttg gtcgaaacgc accgcatttt
      541 gcggaatatg gcggtcatcg tcggtattgg gcaaacccat cagtcgggcg acctgcacgc
      601 cggcaggcgt ttgaaccaag gtttcgggcg taccgcattg aaggattttc ccctcgtgca
      661 tgacggcgat ttcgtccgcc gccgtgcagg cctcttcggg cgaatgcgtt accaaaacgg
      721 cagggatgcc gccgtttcgg atacgttcgg cagtcatgcg gcgcagccgg tcgcgcaaat
      781 gcgtgtccaa actggaaaac gattcgtcca gcaacagcaa ggaagggcgg acaaccaaag
      841 cgcgcgccag tgccaaccgt tgcttctcgc ctccggaaag tttttcaggc ttgcggtgcg
      901 cctcattttc cagtccgact tcggcaagtg ccaacaaggc gaggctttcg gcttcggctt
      961 tcggcatttt ttgcattttc aaaccgaatg ccgcattttc cagcgcactc atatggggaa
     1021 acagcgcgta atcttgaaac atcagcgaaa tacggcgttt ttcgggcggc atacgggtaa
     1081 tgttttcccc gttcagctga atttcgccgc cgtccggccg gacgatgccc gcaatcatat
     1141 tcagcagggt ggattttccg cagcccgacc gccccagtac ggcgagtatt ttgccgcgcc
     1201 cgacagtcag gcagatgttg tcggcaaccg ttttgccgcc gaagcgtttg cagagtccgt
     1261 tcagttcaag catggcgcat cctataaacg tatgccgtcc agccactcgg acagcggatg
     1321 ggcggcatca atcagaccgt aacggcaaaa cgcgtccagc gtaaccagtt gcgcgtcgtg
     1381 catcatgttt cccgacaaca tggtggcaac caagctgccg atgtccattt tttcaaaaca
     1441 cgcaacctcg ccgtcctgat tttcaggcag gaaggtttcg ggtaggacgg catcgaatac
     1501 atacaggatt tcattgtgca caccccggct gacggggcgc aggctgtgca gccgggatac
     1561 cggacggata aacggaaaca gcgttttatc caaaccggct tcttcgctgc tttcgcggca
     1621 cacggcttca gacggcattt cgccgccgga aacaccgccg gcggcaatat tgtcgagttt
     1681 gtcgggatcg actgctttgt gcggactgcg cttgcctatc cagaaatgcc atcggccgtc
     1741 cgattcggtc agtccgttga gatggacggc gcggctgagc agtccgaacg gacggaaagc
     1801 ggcgcgttcg agcgtgaaca aggggttgcc gccgccgtcg gtcaggtcga aacactcgtt
     1861 gcgccatccg tgaagcagcc ccgccttgtt ccatgtgcgg gcgaggtgct gcaagcggcc
     1921 gcccatatct gaccagccgt ccgcattcag gaaaatgccg tctgaagact ccgagcagcc
     1981 tgcctcccag tcttttttga cgcgctccgc ccattccggc gacagattgc ccaaaggcag
     2041 acggttcaga tacagcgttt tccagcaact ttctgcaccg taacttgctt ttgcccactc
     2101 gaacagggca tcaaggtcgt gtttgctgac ggattcggta aaacggacgg tcggcatagt
     2161 cggctttctt gaagacaatg cggaagattg tagccaaact gcctgaattt gcaaccgcaa
     2221 acagacggcg ggctgccgtt ccgatattgc ggcaggaagg aaaatccggc aaaaaacgga
     2281 ggcggcagga aacaggcaaa acaaaacccc tcggcattga accgaggggt ttgatatttg
     2341 gttgcgggag caggattcga acctacgacc ttcgggttat gagcccgacg agctaccatg
     2401 ctgctccatc ccgcgtcaga agatgaaact atacggcaga ttttttatgt tgtcaaacat
     2461 tatttccgcg aaaaatcata aaattttgcc gtccggcggt ttatgcctga tttgaaatat
     2521 cattttcttt acaaaagttc atgttcgtga tttaattttg gttaacattg aaacaggggt
     2581 gctgcatgat gttcaggcgg ctgagaaata ccctttacac ccgatctgga taatacctgc
     2641 gtggggagtt ttcacggatt ctgcctttca gacggcattt tttgatactc gggccgtttg
     2701 aaaaagcaaa gtgctcctgt ttctttattc taaacgagaa aacaggagca ttttttatga
     2761 ctacgccaaa aaaaactgcc aaaacttccg gcaacgaagc acgcgagctt gccgacttga
     2821 gcgaagacat cggcatccgc ttcaaatatc cgaactcgga acgcgtgtat ctgcaaggca
     2881 gccgcgacga catccgcgtg cctttgcgcg aaatccgcca ggacgacacc tacacggcgc
     2941 aaggcacgga agccaatccg ccgattcccg tctatgacac cagcggcgtg tacggcgatc
     3001 cggcggcaca catcgattta aaacaagggc tgccccacat ccgcacggcg tggctggacg
     3061 aacgcggcga taccgaaatc ctgcccaaac tctccagcga atacggcatc gaacgcgcgc
     3121 acgacccgaa aaccgcccat ctgcgtttca accaaatcac ccgcccgcgc cgcgccaaag
     3181 ccggacgcaa cgtaacccaa ttacattatg cccggcaagg cattatcacg cccgaaatgg
     3241 agtttgtcgc catacgcgaa cgtttaaaat tagacgaatt gtcccaaaaa ccggaatacg
     3301 ccaagctctt gaaacaacac gcagggcaaa gtttcggcgc gaatatcccg acccatcccg
     3361 accaaatcac gcctgaattc gtgcgccgag aaatcgccgc cggacgcgcg attattcccg
     3421 ccaacatcaa ccaccccgaa ctcgaaccga tgattatcgg ccgcaacttc cgcgtcaaaa
     3481 tcaacggcaa tttgggcaac tccgccgtca cctccagcct gaccgaagaa gtcgaaaaaa
     3541 tggtgtggtc gctgcgttgg ggcgcggaca cgattatgga tttgtccacc ggcgcgcaca
     3601 tccatgaaac gcgcgaatgg attatccgca acgcgcccgt ccccatcggc accgtgccga
     3661 tttaccaagc gttggaaaaa accggcggca tcgccgaaga tttgacttgg gatttgttcc
     3721 gcgacaccct catcgaacag gcggaacaag gcgtggacta tttcaccata cacgcgggcg
     3781 tgttgctgcg ttatgtaccg atgaccgcca accgcctcac cggcatcgtc tcgcgcggcg
     3841 gttcgattat ggcgaaatgg tgtttggcac atcatcggga aaacttcctc tacacgcatt
     3901 tcgacgaaat ctgcgaaatt atgaaagcgt atgacgtatc gttcagcctc ggcgacggcc
     3961 tgcgccccgg ctgcattgcc gatgccaacg acgaatccca attcgccgag ctgcacacct
     4021 tgggcgaatt gacctccaaa gcgtggaaac acgacgtaca ggtgatgatc gaaggccccg
     4081 gccatgtacc gctgcaacgc gtcaaagaaa atatgaccga agagttgcaa cactgttttg
     4141 aagcaccgtt ttacaccctc ggcccgctcg ttaccgacat cgcccccggc tacgaccaca
     4201 tcacctcggg cataggcgct gccaatatcg gctggtacgg cacggcgatg ctctgttacg
     4261 ttacccccaa agaacatttg ggactgcccg acaaagaaga cgtgcgtacc ggcatcatca
     4321 cctacaaact cgccgcccac gccgccgacc tcgccaaagg ctggccgggc gcacagttgc
     4381 gcgacaacgc cctgagcaag gcgcgtttcg agttccgctg gcgcgaccag ttccgcctaa
     4441 gcctcgaccc cgaacgcgcc gaaagcttcc acgacgaaac cctgcccgcc gaaggcgcga
     4501 aaatcgccca cttctgttcg atgtgcggcc ccaaattctg ctcgatgaaa atcacgcagg
     4561 aagtgcgcga ctacgccgac aagcaaaaag cccaacggca gggcatggag gaaaaagcgg
     4621 tcgagttcgt caaaaaaggc gcgaagattt acagttgaaa cgtcaagcaa aaaatgccgt
     4681 ctgaaacccg gaaaaaaggc ttcagacggc attctttcgc ttgtgaaaat atagtggatt
     4741 aacaaaaatc aggacaaggc gacgaagccg cagacagtac agatagtacg gaaccgactc
     4801 acttggtgct tcagcacctt agagaatcgt tctctttgag ctaaggcgag gcaacgccgt
     4861 actggttttt gttaatccac tataattttt aaaattttta tattcacata gatggggatg
     4921 atgggattta gggttctgat tttgtttttg agagaatgaa ggaatttgag attgtgggcg
     4981 attatcggga aaaatagaat ctttccgccg tcattcccgc gcaggcggga atctagacat
     5041 tcaatgctaa ggcaatttat cgggaatgac tgaaactcaa aaa
//
LOCUS       DAWFHY010000112         4939 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.112, whole genome shotgun sequence.
ACCESSION   DAWFHY010000112 DAWFHY010000000
VERSION     DAWFHY010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.112"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..1358)
                     /locus_tag="WGQ68_001900"
     CDS             complement(<1..1358)
                     /locus_tag="WGQ68_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0715647.1"
                     /translation="MYSNNAITNNRNNITLGAYRDAIISIFSDENVVRAIGTINSMYE
                     EYLTRSHKIDQKEFLREGERLSAKAREFQRTAEEAQKSLKEGIGRAASKFRGNSKEAF
                     VARKVQSDLQKALNASNYNKQQYARRAATALENASKSKVMAANLGKFTYGATVAMGTL
                     SMGSAWWKALDGEGNWSDVGLSSASFLGGLMGVQWGAALGVGAFGLLTGGAGWLVVGA
                     ILGGGAGAWWGSLGAEKAWRWFEDFLNLNRSDKYHVYDPLALDLDGDGIETVATKGFS
                     GSLFDHTNNGIRTATGWVSADDGLLVRDLNGNGIIDNGAELFGDNTKLADGSFAKHGY
                     AALAELDSNGDNIINAADAAFQTLRVWQDLNQDGISQANELRTLEELGIQSLDLAYKD
                     VNKNLGNGNTLAQQGSYTKTDGTTAKMGDLLLAADNLHSRFKDKVELTAEQAKAANLA
                     GIG"
     gene            2632..4764
                     /locus_tag="WGQ68_001901"
     CDS             2632..4764
                     /locus_tag="WGQ68_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEZ0715648.1"
                     /translation="MSIVSAPLPALSALIILAHYHGIAANPADIQHEFCTSAQSDLNE
                     TQWLLAAKSLGLKAKVVRQPIKRLAMATLPALVWCDDGNHFILAKTDGEGEHAQFLIQ
                     DLVTNKSAVLSFAEFSNRYSGKLILVASRASVLGSLAKFDFTWFIPAVIKYRRLFFEV
                     LVVSVVLQLFALITPLFFQVVMDKVLVHRGFSTLDVVSVALLVVSLFEIVLGGLRTYL
                     FAHTTSRIDVELGARLFRHLLSLPLSYFEHRRVGDTVARVRELEQIRNFLTGQALTSV
                     LDLAFSFIFLAVMWYYSSTLTWVVLASLPAYAFWSAFISPILRTRLNDKFARNADNQS
                     FLVESITAVGTVKAMAVEPQMTQRWDNQLAAYVASGFRVTKLAVVGQQGVQLIQKLVT
                     VATLWIGARLVIEGKLTVGQLIAFNMLSGQVAAPVIRLAQLWQDFQQVGISVARLGDI
                     LNAPTENASSHLALPDIRGEITFEHVDFRYKADGRLILQDLNLRIRAGEVLGIVGRSG
                     SGKSTLTKLVQRLYVPEQGRVLVDGNDLALAAPAWLRRQVGVVLQENVLLNRSIRDNI
                     ALTDTGMPLERIIEAAKLAGAHEFIMELPEGYGTVVGEQGAGLSGGQRQRIAIARALI
                     TNPRILIFDEATSALDYESERAIMQNMQAICANRTVLIIAHRLSTVKTAHRIIAMDKG
                     RIVEAGTQQELLAKPNGYYRYLYDLQNG"
BASE COUNT         1243 a         1095 c         1143 g         1458 t
ORIGIN      
        1 ccaatgcccg caagattggc ggcttttgcc tgttcggcag tgagttccac tttgtctttg
       61 aagcggctgt gcagattgtc ggctgctaaa agtaaatccc ccatttttgc ggttgtaccg
      121 tctgttttgg tatagctgcc ttgctgagcc aaagtgttac cgttaccgag atttttattt
      181 acatctttat aggcgagatc caaagattga atacccaatt cttcaagggt acgcaattca
      241 ttagcttggg aaatgccgtc ctgattgaga tcctgccata cacgcagggt ttggaatgcg
      301 gcgtctgccg cgttgatgat gttgtcgccg tttgaatcca attcggccaa agccgcgtag
      361 ccgtgtttgg caaaagaacc gtctgccagt ttggtgttgt cgccgaagag ttccgcgccg
      421 ttgtcgatga tgccgttgcc gttcaaatcg cgcacaagca gaccgtcatc ggcagaaacc
      481 caaccggtgg cggtgcggat gccgttgttg gtgtgatcaa ataagctgcc tgaaaagcct
      541 ttggtggcca cggtttctat accgtcgccg tctagatcta gggcgagggg gtcgtaaacg
      601 tggtatttgt cgctgcggtt aagatttaag aaatcctcaa accatctcca agctttttca
      661 gctcctaatg atccccacca agctcctgcg ccacctccaa gtatagctcc aactactagc
      721 caacctgcgc caccagtaag taaaccaaaa gccccaacac ccaaagccgc accccattgg
      781 acgcccatta gcccacccaa aaaagaagct gatgagagac caacatcact ccaatttcct
      841 tctccatcca aggctttcca ccaagcactt cccatactca aagttcccat ggcaacagtt
      901 gccccataag taaacttacc caaattcgct gccataactt ttgattttga agcattctct
      961 aacgctgttg ccgcacgtct tgcatattgc tgcttgttat agttgctagc attcaatgct
     1021 ttttgcaaat cactttgtac ttttcttgca acaaatgcct cctttgaatt accacgaaat
     1081 ttactagcag ctcttccaat tccttctttt aagctttttt gtgcctcttc tgctgtgcgt
     1141 tggaattctc ttgccttagc agacagacgc tcaccctccc ttaaaaattc tttttgatct
     1201 atcttgtgcg aacgggtcaa atattcttcg tacattgagt taatcgtacc aatcgcccta
     1261 accacatttt catcagaaaa tatggaaata atagcatccc tatacgcacc tagtgtaata
     1321 ttgtttctat tattagttat agcattattc gaatacataa tagcacctcc aaatttagaa
     1381 ctgaatacgt ttatattctc tgtgtaataa tatcaaataa ttaattaatg gataagtaag
     1441 tccgaaaaaa taacaactaa aataagcaat atactgattc tctcctttat taattagata
     1501 tattgaaaaa caaataaaaa ataaacagaa aacattcatg tgaaaaagcc acctcatatt
     1561 cttatttata agtttaaaat aactaggcat tgtatattta aaaatcggta catccaacgg
     1621 aaacaaacca cataaaaatt tttctttatt ctttattaaa gacatagttt tatttttcaa
     1681 aattagtcca gataatatac taacaattac tattaaaaca ataagcacag cgtaattttt
     1741 tataaaattc attaaaataa taacgaaaat actaaataga ttacatacta taaattgaaa
     1801 tataaaatat tcagtattta atccattttc aaccgcgagt tttttaaaat atccttgctc
     1861 gaaaaaatta aaaaaatcca ttaatacttt cctctcaaga aaactaattt tagtttagtt
     1921 aagataaaat gcaatattac cttttctcca taaaagtatg gagcttatat acataaatta
     1981 tgtgcgaggt agcctgaaaa atatccaata caagatagta ggagatgttt cccttatcac
     2041 tattggccaa tacccaattg gcaggcactc tatttccaaa attttctcaa gaatagttaa
     2101 atactattaa ttagtttaaa aacaataaca tacgtttgta tttatccgtt ttggtctgcc
     2161 tgaaacagac tctatgtcca tacacggaca tgtagacagt ttccttgtta ttttggttgc
     2221 tcgtcattag tgatgatact ttactcctct cttaaaaaat cgtcaagaac tgttatcaat
     2281 ctttaaagtc ttttttaaac aaatatatat acgttcaggc tgtctgaaac acaaaaaaat
     2341 gatgttaccc gaccgtgaca gagtcggggc agccggtacc ttaaccctgc accttaaccc
     2401 tgtgccttaa cggtatatag gaggcacgat aggtgcgagg catccgcatc tatcttgatt
     2461 cctaagccgt tttaccttcc tgaaacgaga acttttccat actttccctt tacaattcct
     2521 gtatagatcc ttacaatgtg ttctgctgtt ttatttatat atttctttct acttaatata
     2581 ttccttttcc agccagccgt tatccacatc caatctaact atattttatt tatgtctatc
     2641 gtatccgcac cgctccccgc cctttccgcc ctcatcatcc tcgcccatta ccacggcatt
     2701 gccgccaatc ctgccgatat acagcatgaa ttttgtactt ccgcacagag cgatttaaat
     2761 gaaacgcaat ggctgttagc cgccaaatct ttgggattga aggcaaaggt agtccgccag
     2821 cctattaaac gtttggctat ggcgacttta cccgcattgg tatggtgtga tgacggcaac
     2881 catttcattt tggccaaaac agacggtgag ggtgagcatg cccaattttt gatacaggat
     2941 ttggttacga ataagtctgc ggtattgtct tttgccgaat tttctaacag atattcgggc
     3001 aaactgatat tggttgcttc ccgcgcttcg gtattgggca gtttggcaaa gtttgacttt
     3061 acctggttta ttccggcggt aatcaaatac cgccggttgt tttttgaagt attggtggtg
     3121 tcggtggtgt tgcagctgtt tgcgctgatt acgcctctgt ttttccaagt ggtgatggac
     3181 aaggtgctgg tacatcgggg attctctact ttggatgtgg tgtcggtggc tttgttggtg
     3241 gtgtcgctgt ttgagattgt gttgggcggt ttgcggacgt atctgtttgc acatacgact
     3301 tcacgtattg atgtggaatt gggcgcgcgt ttgttccggc atctgctttc cctgccttta
     3361 tcctatttcg agcacagacg agtgggtgat acggtggctc gggtgcggga attggagcag
     3421 attcgcaatt tcctgaccgg tcaggcgctg acttcggtgt tggatttggc gttttcgttt
     3481 atctttctgg cggtgatgtg gtattacagc tccactctga cttgggtggt attggcttcg
     3541 ttgcctgcct atgcgttttg gtcggcattt atcagtccga tactgcggac gcgtctgaac
     3601 gataagttcg cgcgcaatgc agacaaccag tcgtttttag tagaaagcat cactgcggtg
     3661 ggtacggtaa aggcgatggc ggtggagccg cagatgacgc agcgttggga caatcagttg
     3721 gcggcttatg tggcttcggg atttcgggta acgaagttgg cggtggtcgg ccagcagggg
     3781 gtgcagctga ttcagaagct ggtgacggtg gcgacgttgt ggattggcgc acggctggta
     3841 attgagggca agctgacggt ggggcagctg attgcgttta atatgctctc gggacaggtg
     3901 gcggcgcctg ttatccgttt ggcgcagttg tggcaggatt tccagcaggt ggggatttcg
     3961 gtggcgcgtt tgggggatat tctgaatgcg ccgaccgaga atgcgtcttc gcatttggct
     4021 ttgcccgata tccgggggga gattacgttc gaacatgtcg atttccgcta taaggcggac
     4081 ggcaggctga ttttgcagga tttgaacctg cggattcggg cgggggaagt gctggggatt
     4141 gtgggacgtt cggggtcggg caaatccaca ctcaccaaat tggtgcagcg tctgtatgta
     4201 ccggagcagg gacgggtgtt ggtggacggc aacgatttgg ctttggccgc tcctgcctgg
     4261 ctgcggcggc aggtcggcgt ggtcttgcag gagaatgtgc tgctcaaccg cagcatacgc
     4321 gacaatatcg cgctgacgga tacgggtatg ccgctggaac gcattatcga agcagccaaa
     4381 ctggcgggcg cacacgagtt tattatggag ctgccggaag gctacggcac cgtggtgggc
     4441 gaacaagggg ccggcttgtc gggcggacag cggcagcgta ttgcgattgc ccgcgcgtta
     4501 atcaccaatc cgcgcattct gatttttgac gaagccacca gcgcgctgga ttatgaaagt
     4561 gaacgagcga ttatgcagaa catgcaggcc atttgcgcca accggacggt gctgattatc
     4621 gcccaccgtc tgtccactgt taaaacggca caccggatca ttgccatgga taaaggcagg
     4681 attgtggaag cgggaacaca gcaggaattg ctggcgaagc cgaacggata ttaccgctat
     4741 ctgtatgatt tacagaacgg gtagaaggcg ggggatgggc gtgtaggctg cttttttcgt
     4801 aacgttctgc gttttcagac cgtcgtttac tgggtttact gactgaaatg aaaccaaagt
     4861 gcaggctgcc ggaaagactt tttctgtctt tttaggcagc ctgcacttct ttttcactca
     4921 tctagagacc tttgcaaaa
//
LOCUS       DAWFHY010000113         4802 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.113, whole genome shotgun sequence.
ACCESSION   DAWFHY010000113 DAWFHY010000000
VERSION     DAWFHY010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4802
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.113"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(551..796)
                     /locus_tag="WGQ68_001902"
     CDS             complement(551..796)
                     /locus_tag="WGQ68_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715649.1"
                     /translation="MLKSIELNSHIRNRLAAYLKGRGMDFQTAMREEKGNKEIASIVH
                     SGLPTLVRKLYSEQKMQKFFWEKRDLIADYISRRMQG"
     gene            908..1336
                     /locus_tag="WGQ68_001903"
     CDS             908..1336
                     /locus_tag="WGQ68_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013448419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEZ0715650.1"
                     /translation="MMFSWFKLFHLFFVISWFAGLFYLPRIFVNMAMIDAPRGNPEYV
                     RLSDMAVRLYRFMSPLGFGAVVFGAAIPFAAGWWGSGWVHVKLCLGLILLAYQLYCGV
                     LLRRFQDYSNAFSHRWYRVFNEIPVLLMVAALYLVVFKPF"
     gene            1348..1824
                     /locus_tag="WGQ68_001904"
     CDS             1348..1824
                     /locus_tag="WGQ68_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002228964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEZ0715651.1"
                     /translation="MPIEIERRFLIGNDNWRQYADEPLLLRQGYLSVEKERTVRVRIA
                     GQRAWLTLKGYISEISRSEFEYEIPLADAEKMMETMCPFKMEKWRYPVRWGGSLFEID
                     VFLGDNAPLVVAEIELPDENADFDRPDWLGREITADGMFTNAYLSRHPFSSWKNAV"
     gene            complement(2174..2662)
                     /locus_tag="WGQ68_001905"
     CDS             complement(2174..2662)
                     /locus_tag="WGQ68_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEZ0715652.1"
                     /translation="MLKITLIAACAENLCIGAGNAMPWHIPEDFAFFKAYTLGKPVIM
                     GRKTWESLPVKPLPGRRNIVISRQADYCAAGAETAASLEAALALCAGAEEAVIMGGAQ
                     IYGQAMPLATDLRITEVDLSVEGDAFFPEIDRTHWKEAGRTERRVSSKGTRYAFVHYL
                     RY"
     gene            complement(2730..3785)
                     /gene="aroG"
                     /locus_tag="WGQ68_001906"
     CDS             complement(2730..3785)
                     /gene="aroG"
                     /locus_tag="WGQ68_001906"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HEZ0715653.1"
                     /translation="MTHHYPTDDIKIKEVKELLPPIAHLYELPISKEASGLVHRTRQE
                     ISDLVHGRDKRLLVIIGPCSIHDPKAALEYAERLLKLRKQYENELLIVMRVYFEKPRT
                     TVGWKGLINDPHLDGTFDINFGLRQARSLLLSLNNMGMPASTEFLDMITPQYYADLIS
                     WGAIGARTTESQVHRELASGLSCPVGFKNGTDGNLKIAIDAIGAASHSHHFLSVTKAG
                     HSAIVHTGGNPDCHVILRGGKEPNYDAGHVSEAAEQLRAAGVTDKLMIDCSHANSRKD
                     YTRQMEVAQDIAAQLEQDGSNIMGVMVESHLVEGRQDKPEVYGKSITDACIGWGATEE
                     LLALLAGANKKRMARAG"
     gene            complement(3947..4174)
                     /locus_tag="WGQ68_001907"
     CDS             complement(3947..4174)
                     /locus_tag="WGQ68_001907"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715654.1"
                     /translation="MGNTRIPIKSFFKQLVLFVFNIFLAYEILPDVQAESAAGCSVPD
                     CRGNGKTDVPCGFVRMNGGYCFYQSVSFSLE"
     gene            4110..4342
                     /locus_tag="WGQ68_001908"
                     /pseudo
     CDS             4110..4342
                     /locus_tag="WGQ68_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212266.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4401..4709
                     /locus_tag="WGQ68_001909"
     CDS             4401..4709
                     /locus_tag="WGQ68_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715655.1"
                     /translation="MTPWADAYADLRGKTKVMTTQMCASRDVSKSAKGWSVGIGLNVG
                     KQLTDSVGLEFDPYYRHKTICKPREIVLDGDKTKMGRSKSNEYGFRVTATFYSQLKSK
                     "
BASE COUNT         1177 a         1226 c         1132 g         1267 t
ORIGIN      
        1 ttttaatgct gcaagaattt atcggggatg accgaggttt gaaggtctgg attcccgcct
       61 gcgcgggaat gacggtttag aaatcacccg aaacgagaaa aagaaccgaa accgaacaag
      121 ccggattccc acctgcgcgg gaatgacggg atttcaggtt actgtttttg attttctgtt
      181 ttgtgggaat aacgggattt tggatgatgt gcattttcgg gaaaactaaa aaccctccgc
      241 cgtcattccc gcgaaagtgg gaatctagaa ttttaatgcg gcaagaattt atcggaaaaa
      301 actgaagttt aaagacctag attcccgcct gcgcgggaat gacggtttag aaatcacccg
      361 aaacgagaaa aagaaccgaa accgaacaag ccggattccc acctgcgcgg gaatgacggc
      421 tcaaaagtta cgaaacgaaa aataaccgaa accagacaag tcggattccc gcgcgggcgg
      481 gaatgacgat aagcgtaatt tcaatctgcc atattgtctt ttcacttgcc tgaaaacaga
      541 tttcagccgc ctatccctgc atccggcggc tgatgtagtc ggcaatcaaa tcccgctttt
      601 cccaaaaaaa cttctgcatt ttttgttcgg aatacagttt gcggaccaga gtgggcagac
      661 cgctgtggac gatggaggcg atttctttgt tgcctttttc ctcccgcatc gccgtctgaa
      721 aatccatacc cctgcctttc agatatgctg caaggcggtt gcggatgtgg gaattgagtt
      781 cgatggattt gagcattttg cctccgtttt gcggtaagga tttattcgat ttgtttatcc
      841 gcctgatttt atcacgtttc ccaaacggtt tgcctgtaca ataccgcttt tcggcagggt
      901 gtgggcgatg atgttttctt ggttcaagct gtttcacttg ttttttgtca tttcgtggtt
      961 tgcagggctg ttttacctgc cgaggatttt cgtcaatatg gcgatgattg atgcgccgcg
     1021 cggcaatccc gagtatgtgc gcctgtcaga tatggcggtg cggctgtacc gttttatgtc
     1081 gccgttgggc ttcggcgcgg tcgtgttcgg cgcggcgata ccgtttgccg ccggctggtg
     1141 gggcagcggc tgggtacacg tcaaactgtg tttgggcttg atactcttgg cttaccagtt
     1201 gtattgcggc gtgctgctgc gccgttttca ggattacagc aatgcttttt cacaccgctg
     1261 gtaccgcgtg ttcaacgaaa tccccgtgct gctgatggtt gccgcgctgt atctggtcgt
     1321 gttcaaaccg ttttgacgga gcatcttatg ccgatagaaa tcgaacgccg ttttttgatt
     1381 ggaaacgaca actggcggca atacgccgac gaaccgctgc tgttgaggca gggttatctg
     1441 tctgttgaaa aagaacgcac cgtccgtgtc cgcattgccg gacaacgggc gtggctgacg
     1501 ctgaaaggct atatttcgga aatcagccgc agtgagtttg aatacgaaat cccgcttgcc
     1561 gatgcggaaa agatgatgga aacgatgtgc ccgtttaaaa tggaaaaatg gcgttatccg
     1621 gtcagatggg gcggcagcct gtttgaaatc gatgtttttc ttggcgataa tgcgcctttg
     1681 gtcgtcgcgg aaatcgagtt gcccgacgag aatgccgatt ttgaccgtcc ggactggctg
     1741 ggacgggaaa tcactgccga cggtatgttt accaatgctt atttgagcag gcatccgttc
     1801 tcaagctgga aaaatgccgt ctgaaacgac ttcagacggc attctccgac aaccatcgca
     1861 aatccaaaga cagcctctat atccttattt ctgctttctt tgttccatta ttcccaataa
     1921 atccccataa cctaaaatct cgtcattccc gcgcaggcgg gaatccggtt ctttaaactt
     1981 cggttttttc cgataaattg ctttagcttt tcgtttccgg attcccgctt ttgcgggaat
     2041 gacgatggaa gattgttgtt atttcggata aattacccta actttaaaat cccggattcc
     2101 catcttcggg ggaataacaa tattttctat ttcagcccat catttgcggg ggattttata
     2161 gagagtttat atttcaatat ctcaaataat gcacaaaagc atagcgcgtg cctttgctgc
     2221 tgacgcggcg ttccgtccgc cctgcctctt tccaatgcgt ccggtctatt tcggggaaaa
     2281 atgcatctcc ttccacagac aaatccactt cggttatccg caaatcggtc gccaatggca
     2341 tcgcttgtcc gtatatctgc gcgccgccca taatgacagc ttcttccgcg cctgcgcaca
     2401 atgccaatgc cgcctccaaa cttgccgccg tttccgcgcc tgccgcgcaa taatccgcct
     2461 gccggctgat gacgatgttc ctccgtccgg gcaggggttt gacgggcagg gattcccacg
     2521 ttttccgccc cataatgacg ggtttgccca aggtataggc tttgaaaaat gcgaaatctt
     2581 cggggatgtg ccaaggcata gcattgcccg ccccgatgca caggttttcc gcacacgccg
     2641 caattagggt tattttgagc atatttcctc cacaaaaatg ccgtctgaac gcttcagacg
     2701 gcattttatg acattccgtg tcaaaaatct caaccggcgc gcgccatacg ttttttgttt
     2761 gcacctgcca acaatgccaa cagttcttca gtcgcgcccc aaccgataca cgcatcggta
     2821 atgctcttgc cgtacacttc cggcttgtcc tgcctgcctt cgaccaaatg gctttccacc
     2881 atcacgccca tgatattgct cccgtcctgt tccaattggg cagcaatatc ttgtgccact
     2941 tccatctgcc gggtgtaatc cttgcggctg ttggcgtggc tgcaatcgat catcagcttg
     3001 tcggttaccc ctgccgcacg cagttgttcc gccgcctcgc tgacgtgtcc cgcatcataa
     3061 ttcggctctt ttccaccgcg caaaatgaca tgacagtcgg gattgccgcc ggtatggaca
     3121 atggcggaat gcccggcctt ggttacagac aggaaatgat gcgaatggct cgccgcaccg
     3181 attgcgtcga tggcaatctt caaattgccg tccgtaccgt ttttaaagcc gacggggcag
     3241 gacagcccgc ttgccaattc gcggtgaact tggctttcgg tcgtccgcgc gccgattgcc
     3301 ccccaagaaa tcaagtccgc ataatattgc ggcgtaatca tatccaaaaa ctcggtagag
     3361 gcaggcatac ccatattgtt caacgacaac aacaggctgc gcgcctgacg caaaccgaaa
     3421 ttgatgtcaa acgtaccgtc caaatgcggg tcgttaatca aacctttcca acccaccgtc
     3481 gttctcggct tctcgaaata aacgcgcatc acaatcaaaa gctcgttttc atactgcttg
     3541 cggagtttca acaaacgctc cgcatattcc aatgccgctt tcggatcgtg aatcgaacac
     3601 ggtccgataa taaccaacag ccgcttgtcc ctgccgtgaa ccaaatcgga aatttcctga
     3661 cgggtgcggt gaaccaagcc cgaagcctct ttggaaatcg gcagctcgta aagatgggca
     3721 atcggcggca acaactcttt aacttcttta atcttaatat cgtctgtggg gtaatggtgt
     3781 gtcatcttgc atctttcctg atgggttttt atacgcggct taggttacat cgtttacctt
     3841 gcacaaacaa gtgcaacata ttactacaaa gctattttat ttcaaaataa ctgatatatc
     3901 ttttttcata gattttaaat ttcatatttt taatatttta ggaattttat tccaaagaaa
     3961 aagaaacaga ttgataaaaa caatacccac cattcattcg gacaaaaccg cacggaacat
     4021 ccgttttccc atttccacga caatccggta cagaacaccc cgctgccgat tccgcctgca
     4081 catccggcaa aatttcatac gcaaggaaaa tgttgaaaac aaaaagaact aattgcttga
     4141 aaaaagattt aattggaatg cgggtatttc ccacactttc tccatttatt catcctcatt
     4201 ataggaagaa aaacatgaaa aaaccctgct tgcactctca cttgcagccg tgtccgccgt
     4261 tacggtagcc gatattcctg agtctaacgg caagggcaaa gccatcatcg gcgataagga
     4321 aaaacaccgt taccaagcat gaaaaccttg ttttgaaagt ctatggcgaa atccgccaag
     4381 atttggggga cggatgatcc ttgacccctt gggcggatgc atatgcagat ttgcgcggca
     4441 aaaccaaagt gatgacgacc cagatgtgtg cttcccgcga tgtcagcaaa agcgccaaag
     4501 gttggagtgt cggtatcggt ctgaatgtag gcaaacagtt gaccgacagc gtcggtctcg
     4561 agtttgatcc atactaccgt cacaaaacaa tctgcaaacc ccgtgagatt gttttggacg
     4621 gcgacaaaac caaaatgggc cgctccaaat ccaacgagta cggcttccgc gtaaccgcaa
     4681 cgttctatag tcaattaaaa tcaaagtagg acagtagtgc atcgtcaaat cgggcgtaat
     4741 cagacaaaac ggttcgcaga taccgcttaa tattcgccca cactttctca atcgggttga
     4801 gc
//
LOCUS       DAWFHY010000114         4430 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.114, whole genome shotgun sequence.
ACCESSION   DAWFHY010000114 DAWFHY010000000
VERSION     DAWFHY010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.114"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            53..475
                     /locus_tag="WGQ68_001910"
     CDS             53..475
                     /locus_tag="WGQ68_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715656.1"
                     /translation="MSAVFIAPYLTAFHEQEKTFEYAELTVTAPNRSGRAIKLDADGQ
                     HYRLSCYGFDSLCTGGNIGRTIRVEQVRAVLSETVGKGFLNGVLLEYRNSGSVYSNKD
                     FARTEDRLVEVLAQPAVFCLKLGILLLLPAIFLRLKRM"
     gene            581..1144
                     /gene="pgsA"
                     /locus_tag="WGQ68_001911"
     CDS             581..1144
                     /gene="pgsA"
                     /locus_tag="WGQ68_001911"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEZ0715657.1"
                     /translation="MPWNLPIFLTWLRVLLIPVMTALFYLPFPWFAEETINLAAAVIF
                     AVAALTDWFDGFLARLWKQTSDFGAFLDPVADKLMVAVSLLLLVKLDRTYVLFAMIII
                     GREITISALREWMAQMGKRNSVAVATVGKFKTAAQMLAIFLLLLNIPDFYGFNLVVIG
                     NVLMFIASLLTVWSMLYYLKMAWKEIA"
     gene            1224..1299
                     /locus_tag="WGQ68_001912"
     tRNA            1224..1299
                     /locus_tag="WGQ68_001912"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1257..1259,aa:Gly,seq:gcc)
     gene            1325..1400
                     /locus_tag="WGQ68_001913"
     tRNA            1325..1400
                     /locus_tag="WGQ68_001913"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1358..1360,aa:Gly,seq:gcc)
     gene            1424..1499
                     /locus_tag="WGQ68_001914"
     tRNA            1424..1499
                     /locus_tag="WGQ68_001914"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1457..1459,aa:Gly,seq:gcc)
     gene            1521..1596
                     /locus_tag="WGQ68_001915"
     tRNA            1521..1596
                     /locus_tag="WGQ68_001915"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1554..1556,aa:Gly,seq:gcc)
     gene            1622..1695
                     /locus_tag="WGQ68_001916"
     tRNA            1622..1695
                     /locus_tag="WGQ68_001916"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1654..1656,aa:Cys,seq:gca)
     gene            1796..1885
                     /locus_tag="WGQ68_001917"
     tRNA            1796..1885
                     /locus_tag="WGQ68_001917"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1830..1832,aa:Leu,seq:taa)
     gene            1989..3041
                     /gene="sohB"
                     /locus_tag="WGQ68_001918"
     CDS             1989..3041
                     /gene="sohB"
                     /locus_tag="WGQ68_001918"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003709418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HEZ0715658.1"
                     /translation="MWKEILLNYGIFLLELLTVFGAIALIVLAIVQSKKQSESGSVVL
                     TDFSENYKKQRQSFEAFFLSGEEAKHQEKEEKKKEKAEAKAEKKRLKEGGEKSSETQK
                     SRLFVLDFDGDLYAHAVESLRHEITAVLLIAKPEDEVLLRLESPGGVVHGYGLAASQL
                     RRLRERNIPLTVAVDKVAASGGYMMACVADKIVSAPFAIVGSVGVVAEVPNIHRLLKK
                     HDIDVDVMTAGEFKRTVTFMGENTEKGKQKFRQELEETHQLFKQFVSENRPQLDIEEV
                     ATGEHWFGRQALALNLIDEISTSDDLLLKAFENKQVIEVKYQEKQSLIQRIGLQAEAS
                     VEKLFAKLVNRRADVM"
     gene            complement(3092..3958)
                     /locus_tag="WGQ68_001919"
     CDS             complement(3092..3958)
                     /locus_tag="WGQ68_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter six-transmembrane
                     domain-containing protein"
                     /protein_id="HEZ0715659.1"
                     /translation="MWKMLKHIAQTHRKRLLGTFSLVGLENLWMLVYPVFGGRAINAV
                     IAGEVWQALLYALVVLLMWLVGAARRIADTRTFTRIYTEIAVPVVLEQRQRQVPHSAV
                     TARVALSREFVSFFEEHLPIAATSVVSIFGACIMLLVLEFWVGVSAVGILALFLWLLP
                     RFAAISENLYFRLKNSLERDNHFIRKGDERQLDRHYGLLARLRVLISNREAFGYLCVG
                     TAMGILFGFAFVMMTLKGYSSAGHVYSVGTYLWMFAMSLDDVPRLVEQYSNLKDIGQR
                     IEWSKRNIKAGT"
     gene            complement(3968..4399)
                     /locus_tag="WGQ68_001920"
     CDS             complement(3968..4399)
                     /locus_tag="WGQ68_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEZ0715660.1"
                     /translation="MNQLDQLGTRINLICNVFDKWIGQQDLNYNLFAVLYTLATEGSR
                     TQKHIGEEWSLPKQTVSGVCKTLAGQGLIEWQESEQDRRERLLSLTEKGKAHAAPLTE
                     SAQEFSDKVFATFGDKRTTRLFADLDALAEVMEKTISENKK"
BASE COUNT         1087 a         1052 c         1161 g         1130 t
ORIGIN      
        1 taatccacta tattatcgct gggtatggtt tgccgccata agctgtctgc tgttgtccgc
       61 cgtttttatt gcaccttacc tgacggcatt tcacgaacaa gaaaaaacat tcgaatatgc
      121 ggaattgacc gttaccgccc ccaaccgcag cggacgggca atcaaactgg atgcagacgg
      181 gcagcattac cggctttcct gctatgggtt cgacagtttg tgcacaggcg gcaatatcgg
      241 cagaaccatc agggtggagc aggttagagc cgttttaagc gaaaccgtcg gcaaaggttt
      301 tttaaacggc gttctgttgg aataccgcaa cagtggcagt gtctatagca ataaagattt
      361 tgcccgcacg gaagaccgcc ttgtcgaagt gttggcacaa cctgccgttt tctgcctgaa
      421 actgggtatt ttgcttttat tgcccgccat tttcctgcgt ttgaaaagaa tgtgaaaaca
      481 gattggcggg gcgggggaat gcccatgcag caattttacg ttttgggtta tgaggataac
      541 cgttataatc acaactttac agtctgcaca gagaaaaccg atgccttgga atctccccat
      601 cttcctcaca tggttgaggg tcttgctcat tcctgtcatg acagcccttt tttacctgcc
      661 ttttccgtgg ttcgcggagg aaacgattaa tcttgccgcc gccgtcattt ttgccgttgc
      721 cgccttaacc gactggtttg acggattttt ggcaagactg tggaaacaga cttccgattt
      781 cggcgccttc ctcgatcccg ttgccgacaa gctgatggtc gccgtctctt tgcttttact
      841 ggtcaaactc gaccggacct atgttttgtt tgccatgatc atcatcggca gggaaattac
      901 catttccgca ttgcgcgaat ggatggcgca aatgggcaaa aggaacagcg ttgccgtcgc
      961 caccgtcggt aagtttaaaa ccgccgcgca aatgctggca attttcctgc tgctgctgaa
     1021 tattcccgat ttttacggat ttaatcttgt tgttatcggc aatgtattga tgtttatcgc
     1081 atctttgctg acagtctggt cgatgctgta ttatctgaaa atggcgtgga aagaaatcgc
     1141 ctgaaaaaaa cataaaaata gcttgacggc aaaaacagaa tccataataa ttgcgtctct
     1201 tcgatgttgg agaatgaaat cgggcgggaa tagctcagtt ggtagagcgc aaccttgcca
     1261 aggttgaggt cgcgagttcg agactcgttt cccgctccaa aaatttcatt ttcccacaat
     1321 cattgcggga atagctcagt tggtagagcg caaccttgcc aaggttgagg tcgcgagttc
     1381 gagactcgtt tcccgctcca agttttattt caaacatatc aacgcgggaa tagctcagtt
     1441 ggtagagcgc aaccttgcca aggttgaggt cgcgagttcg agactcgttt cccgctccaa
     1501 aaaccgatat gtttgaacgc gcgggaatag ctcagttggt agagcgcaac cttgccaagg
     1561 ttgaggtcgc gagttcgaga ctcgtttccc gctccaagtt ttttagataa gccagtttta
     1621 gggcgagata gcaaagtggt tatgcagcgg attgcaaatc cgtctacgcc ggttcgattc
     1681 cgactctcgc ctccatatat attacggcgg ggtggcagag tgtttatgct atgaggagtg
     1741 caatcttcat ataggccggt taaaatccgc gcccccgcct ccacctttca caaatgcccg
     1801 ggtggtgaaa taggtagaca caacggactt aaaatccgtc gggactaaac atcccgtgcc
     1861 ggttcgattc cggctccggg caccaagctg aaaatgacaa tgccgctcca agcggttatt
     1921 tttttatctg tcggacggtg atggacaata ttgacatgtt catgcctgaa caagaggaaa
     1981 tccaatcaat gtggaaagaa attttactga attacggtat tttcctgctc gaactgctta
     2041 ccgtgttcgg cgcaattgcg ctgattgtgt tggctatcgt acagagtaag aaacagtcgg
     2101 aaagcggcag tgtcgtactg acggattttt cggaaaatta taaaaaacag cggcaatcgt
     2161 ttgaagcatt ctttttaagc ggggaagagg caaaacatca ggaaaaagag gaaaagaaaa
     2221 aggaaaaggc ggaagccaaa gcagagaaaa agcgtttgaa ggagggtggg gagaaatctt
     2281 ccgaaacgca aaaatcccgc ctttttgtgt tggattttga cggcgatttg tatgcacacg
     2341 ccgtagaatc cttgcgtcat gagattacgg cggtgctttt gattgccaag cctgaagatg
     2401 aggttctgct tagattggaa agtccgggcg gcgtggttca cggttacggt ttggcggctt
     2461 cgcagcttag gcgtttgcgc gaacgcaata ttccgctgac cgtcgccgtc gataaggtgg
     2521 cggcgagcgg tggttatatg atggcgtgtg tggcggataa aattgtttcc gctccgtttg
     2581 cgattgtcgg ttcggtgggt gttgtagcgg aagtaccgaa tatccaccgc ctgttgaaaa
     2641 aacatgatat tgatgtggat gtgatgacgg cgggcgaatt taagcgcacg gttactttta
     2701 tgggtgaaaa tacggaaaag ggcaaacaga aattccgaca ggaactggag gaaacgcatc
     2761 agttgttcaa gcagtttgtc agcgagaacc gccctcaatt ggatattgag gaagtggcaa
     2821 cgggcgagca ttggttcggt cggcaggcgt tggcgttgaa cttgattgac gagatttcga
     2881 ccagtgatga tttgttgttg aaagcgtttg aaaacaaaca ggttatcgaa gtgaaatatc
     2941 aggagaagca aagcctgatc cagcgcattg gtttgcaggc ggaagcttct gttgaaaagt
     3001 tgtttgccaa acttgtcaac cggcgggcgg atgtgatgta gtttgccgaa cggatggaaa
     3061 tgccgtctga agcgtgttca gacggcattt ttcaagttcc ggctttgatg ttccgtttcg
     3121 accactctat ccgttgtccg atgtctttca aattggaata ttgttcgacc aatcgcggca
     3181 cgtcgtccaa actcatggca aacatccaca gataagtgcc gaccgaatag acatgccccg
     3241 cgctgctgta gcctttgagc gtcatcatca caaaagcaaa gccgaacaaa atacccatcg
     3301 ccgtgccgac gcagagatag ccgaaggctt cgcggttgga aatcagcaca cgcaggcgcg
     3361 caagcagtcc gtaatggcgg tccagctgcc gctcgtcgcc ttttcggata aagtggttgt
     3421 cgcgttccaa gctgttcttc aggcggaaat acaggttttc gctgatggcg gcaaaacgtg
     3481 gcaaaagcca taaaaacaac gcaagtatgc ccaccgccga cacgccgacc caaaattcca
     3541 gcaccagcag catgatgcac gcgccgaata tggatacgac ggatgtcgcg gcaatcggca
     3601 ggtgttcttc aaaaaagctg acaaactcac gcgacagggc aacccgcgca gttaccgccg
     3661 aatgcgggac ttgtcgctgc cgctgttcca acacgaccgg cacggcgatt tcggtataaa
     3721 tccgcgtaaa cgtgcgcgta tcggcaatcc gccgcgccgc accgaccagc cacatcaaaa
     3781 gcacaaccaa agcgtacagc aacgcctgcc acacctcccc cgcaatcacg gcattgatcg
     3841 cccggccgcc aaacactgga tataccagca tccaaaggtt ttccagtccg accagggaaa
     3901 acgtgccgag cagacgcttg cggtgggttt gggctatgtg tttcaacatt ttccacatat
     3961 ttgctcccta ttttttattt tccgagattg ttttttccat cacttcagcc agtgcatcca
     4021 aatcggcaaa cagccgggtt gtgcgcttgt cgccgaatgt ggcaaatact ttgtcgctga
     4081 attcctgcgc gctttctgtt aaaggtgcgg catgggcttt gcctttttcg gtcagcgaca
     4141 gcaaccgttc gcgccggtcc tgttcgcttt cctgccattc aatcaaccct tgtccggcaa
     4201 gggttttgca tacgcctgaa acggtctgtt tgggcaaact ccactcttcg ccgatatgct
     4261 tttgcgtgcg gctgccttcg gttgccaggg tataaagtac ggcaaagagg ttgtaattca
     4321 gatcctgctg cccgatccat ttgtcgaaga cattgcaaat caggttgata cgggtgccaa
     4381 gttggtcgag ttggttcata attggtcttg atattgacta aatttgtgcg
//
LOCUS       DAWFHY010000115         4417 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.115, whole genome shotgun sequence.
ACCESSION   DAWFHY010000115 DAWFHY010000000
VERSION     DAWFHY010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4417
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.115"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..144)
                     /locus_tag="WGQ68_001921"
     CDS             complement(<1..144)
                     /locus_tag="WGQ68_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715661.1"
                     /translation="MPSETGFQTAFFQTALESQTLFAMPYIPNPIRYKLKTTIQKTEI
                     HIKR"
     gene            complement(151..1284)
                     /locus_tag="WGQ68_001922"
     CDS             complement(151..1284)
                     /locus_tag="WGQ68_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715662.1"
                     /translation="MFKKFKPVLLSFFALVFAFWLGTGIAYEINPRWFLSDTATEIPE
                     NPNAFVAKLARLFRNADRAVVIVKESIRTEENLAGTVDDGPLQSEKDYLALAIRLSRL
                     KEKAKWFHVTEQEHGKEVWLDYHIGEGGLVAVSLSQRSPEAFVNAEYLYRNDRPFSVN
                     VYGGTAHGENYETTGEYRVVWQPDGSVFDASGRGKIGEDVYEHCLGCYQMAQVYLAKY
                     RDVANDEQKVWDFREESNRIASDSRDYVFYQNMRELMPRGMKANSLVVGYDADGLPQK
                     VYWSFDNGKKRQDFEYYLKNGNLFIAQSSTVALKADGVTADIQTYHAQQTWYLDGGRI
                     IREEKRGDRLPDFPLNLENLEKEVSRYAEAAARRSGGRRDLSH"
     gene            complement(1555..1653)
                     /locus_tag="WGQ68_001923"
                     /pseudo
     CDS             complement(1555..1653)
                     /locus_tag="WGQ68_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1689..2066)
                     /locus_tag="WGQ68_001924"
     CDS             complement(1689..2066)
                     /locus_tag="WGQ68_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0715663.1"
                     /translation="MCEFKDIIRNVPYFEGYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRKKYPYPMDIPRYVVIGIGTIIDFLMVPNWKLFEIKASPWLPDSVGINERYERLK
                     TMLRYIFTEKDIVNVRFDYYNKK"
     gene            complement(2097..2495)
                     /locus_tag="WGQ68_001925"
     CDS             complement(2097..2495)
                     /locus_tag="WGQ68_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity 41 family protein"
                     /protein_id="HEZ0715664.1"
                     /translation="MCEFKDFRRNIPCFKEYDENSFIGKWYDDGVWDDEEYWKLENDL
                     IEVRRKYPYPMDIPRDIVIGIGSIIDFLMVQNWKLFEIKASPWLPKSVKINERYERFR
                     VMLRYIFTDLDVDDWKFFYFPIQHSKGRLR"
     gene            complement(2501..3178)
                     /locus_tag="WGQ68_001926"
     CDS             complement(2501..3178)
                     /locus_tag="WGQ68_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BECR-fold containing toxin"
                     /protein_id="HEZ0715665.1"
                     /translation="MVKTADGYKAIAHIQTGEHVFAKDETSGKTGYKPVTARYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLFAESGKTQTVRNIIV
                     KPKPLKAYNLTVADWHTYFVKGNQAETEGVWVHNACPPRKTPSTPVYGNDSEAYAAAK
                     KLGYRKIKERTRNDAAIFKKGKSYISRDVDSHNGGAWKEASSPKNLNRKETRNGTFDK
                     NLNRIGD"
     gene            complement(3314..3859)
                     /locus_tag="WGQ68_001927"
     CDS             complement(3314..3859)
                     /locus_tag="WGQ68_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004463655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="suppressor of fused domain protein"
                     /protein_id="HEZ0715666.1"
                     /translation="MIIVEHLEHYLGEIESGIKCLDRRYHLSVSVFPSQPYKGVTTFS
                     TLGLNRYDLNYKSRFELIFTCSEEWNKENIAAFLSGVAEYLIDNRQPILRGEIIQLPR
                     VIIEGSKMDALYVSAPFYFDDDFQVCYGEHYNIVFPLLVPLYKQEAEWVEKKGWNAFE
                     QFLLDNEVGNLSDMNRKPFTW"
     gene            complement(3846..3998)
                     /locus_tag="WGQ68_001928"
     CDS             complement(3846..3998)
                     /locus_tag="WGQ68_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715667.1"
                     /translation="MDAKPLGGNIKMPLYFSDGISVNKKVGIGVQNSLHLAGIGMKSL
                     RKNDYS"
     gene            complement(3980..4252)
                     /locus_tag="WGQ68_001929"
     CDS             complement(3980..4252)
                     /locus_tag="WGQ68_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715668.1"
                     /translation="MMSVKELFKVILDENKDFPIRTIHRTPIGILPKPVALSIVQYED
                     DQGFYLFYLDETGQEQTDTYHDTLDSAFEQVEFEFGISKEEWMQSP"
BASE COUNT         1214 a         1037 c          770 g         1396 t
ORIGIN      
        1 ccgtttaatg tgtatttccg ttttttggat tgtggttttc aatttatagc gaattggatt
       61 tggaatatac ggcattgcaa aaagcgtttg actctccagt gccgtctgaa aaaatgccgt
      121 ctgaaaaccg gtttcagacg gcatttgcgt tcagtgagaa aggtcgcgcc tgccgcccga
      181 acgtctcgcc gcagcctctg cataacggct cacctctttt tccaaatttt ccaagttcaa
      241 aggaaaatca ggcagtctgt ctccccgttt ctcttcgcgg ataatccgcc cgccatccaa
      301 ataccacgtc tgttgcgcat gataggtctg tatatccgcc gttactccat ccgctttcaa
      361 tgctaccgtc gaagattgtg caataaaaag atttccgttt ttcaaataat attcgaaatc
      421 ctggcgtttt ttcccattgt cgaaactcca atagactttc tgcggcagac cgtccgcatc
      481 atagccgacc acaagactgt ttgccttcat cccccggggc atcaattccc gcatattctg
      541 ataaaacaca taatcacgcg agtccgacgc aatccggttg ctctcttcgc ggaagtccca
      601 aaccttctgc tcatcattcg cgacatcccg atatttcgcc aaatatacct gggccatctg
      661 ataacacccg agacaatgct cataaacatc ttccccgatt tttccgcgcc ccgacgcatc
      721 aaataccgaa ccgtctggtt gccaaacaac ccgatattct cctgtcgttt cataattttc
      781 tccgtgagcc gttccgccgt acacatttac agaaaacgga cgatcgttcc gatacagata
      841 ttcggcatta acaaatgctt ccggcgagcg ttgcgaaagc gaaaccgcaa ccaaaccgcc
      901 ctcgccgata tggtaatcca gccaaacctc tttcccatgt tcctgctccg ttacgtgaaa
      961 ccatttcgcc ttttctttca aacgactgag ccggatagcg agcgcgagat aatccttctc
     1021 cgactgcaac ggaccgtcat ccacagttcc ggcaagattt tcctccgtcc ttatcgattc
     1081 cttcacgatg acaaccgccc tgtcggcatt tcggaacagg cgggcaagtt tcgccacaaa
     1141 agcattcgga ttttcaggta tttcagttgc cgtatcgctc aaaaaccaac gcggattaat
     1201 ctcataggca atacccgttc ccagccaaaa ggcaaataca agtgcaaaaa atgacaacag
     1261 taccggtttg aattttttaa acatatttat ttttcgttta acagaatata tcgattatat
     1321 cagacgagct ttgattgttt gatttttaat ctaatctacg cttgctgccg tactttaaaa
     1381 aaacctatgc aggctgcggc ttgttactct tgtttgcttt gattcaaatt ttcaactaat
     1441 tcaaatgctt aagatcgata ttgcctttgc catttttccc aatttaacgg tcttcccgag
     1501 cgttcccaat cttctaaaac tgatattttg aacgtttcca tttcccacca atattaagtc
     1561 ttctgcctta atccatttgc cgtccgaata aaacggatgg atgcggttgg aaatcagggt
     1621 ttggctgtta ccgatgccgt ctgaaagccg aataccgctt cagacggcat tttgattgcc
     1681 ggggtttgct attttttgtt gtaataatca aatcgcacgt tgactatgtc tttctcggta
     1741 aaaatataac ggagcattgt tttaagcctt tcataacgtt cattaattcc tacgctatca
     1801 ggtagccaag gggaagcttt aatttcaaaa agtttccaat ttggaaccat taagaaatca
     1861 ataatggtac cgattccaat gacaacatat cttggtatat ccatcggata aggatatttt
     1921 tttctaacct caattaaatc attctccaac ttccaatatt cttcatcatc ccacaccccg
     1981 tcatcatacc atttgccaat aaatgaattt tcgtcatacc cctcaaaata aggaacgttt
     2041 cttataatat ccttgaactc acacataata atgtatctcc aatataatta aacttttcat
     2101 ctcaatctac ctttactatg ttgtattgga aagtaaaaaa atttccagtc atctacatct
     2161 agatcagtaa aaatataacg aagcattact ctgaaccttt cataacgctc attaattttg
     2221 acacttttag gcaaccaagg agaagcttta atttcaaaaa gtttccaatt ttgaaccatt
     2281 aaaaaatcaa taatgctacc gattccaatc acgatgtccc ttggtatatc catcggataa
     2341 ggatattttc tcctaacctc gattaaatca ttctccaatt tccaatattc ttcatcatcc
     2401 caaaccccgt catcatacca tttgccaata aatgaatttt cgtcatactc cttaaaacaa
     2461 gggatgtttc ttctaaaatc cttgaactcg cacataataa ttaatctcca atacgattta
     2521 ggtttttatc aaatgtaccg tttcttgttt cttttctgtt cagatttttg ggtgaagatg
     2581 cttctttcca agcccctcca ttatgtgaat ctacatcgcg tgatatataa ctctttcctt
     2641 ttttaaaaat agcagcatca tttctcgttc tttcttttat ttttctatat cccaatttct
     2701 ttgctgctgc atatgcttct gaatcattcc catatacggg ggtagatggt gtttttcttg
     2761 gcggacacgc attatgaacc caaacccctt ccgtttccgc ctgattaccc ttgacgaagt
     2821 aggtatgcca gtcggcaacg gtcagattgt aggctttgag cggttttggt ttgacaatga
     2881 tgttgcggac ggtttgggtt ttgccgcttt cggcaaacag cctgcttccc gctttcaaat
     2941 cttccgcttt aatccatttc ccatccgaat aaaacggatg gatgcggttg gaaatcaggg
     3001 tttggctgtt gccgatgccg tctgaaactt caatgtaaac ggtttcctgg tacggattgc
     3061 cgtatcgggc ggtaacaggt ttgtatccag tttttccact ggtttcatct ttggcaaaga
     3121 cgtgctctcc agtttggata tgtgcgatgg ctttgtagcc gtctgccgtt ttgaccaagg
     3181 tgctgccgtg gaaggagcag gtgtaggatt tttgaagaac ttagttccca atatcctgtt
     3241 taaaataagg ggaaggtcgc ctgaaaatta aacacggttt cagacggcac tttgattaaa
     3301 tttaagaaaa tccttaccaa gtaaacggtt tccgattcat atccgaaagg ttgccaactt
     3361 cattatccag caagaactgc tcaaaagcat tccaaccctt tttttccacc cattcggctt
     3421 cctgtttata caaggggaca agcaaaggga aaacgatatt gtagtgttcg ccataacaga
     3481 cctgaaaatc atcgtcgaaa taaaatgggg cggaaacata cagtgcatcc attttgctac
     3541 cttcgataat taccctgggt aattgaataa tttcacctct taaaataggt tgtctattgt
     3601 caatcaaata ttcggctact cctgatagaa aagctgcaat attttcttta ttccattctt
     3661 cagaacaggt aaaaattaat tcaaacctac tcttatagtt caagtcatat cgattcaagc
     3721 ctaaagtaga aaaagtagtt acccctttat atggttgtga tgggaagacg gaaacgctga
     3781 gatgataacg tcgatccaag cattttatac cgctttcgat ttcacccaaa taatgttcta
     3841 aatgttcaac tataatcatt ttttcttaaa gatttcatcc ctatcccggc aagatgtagg
     3901 ctgttttgga ctccaatacc gactttttta ttgacggaaa tgccgtctga aaaataaagc
     3961 ggcatcttga tattgccacc taagggcttt gcatccattc ttctttgcta attccaaatt
     4021 caaactccac ctgttcaaat gccgaatcta atgtgtcatg ataagtatcc gtttgttcct
     4081 gaccggtctc atccaaataa aacaaataaa atccttggtc atcttcatat tgaacaatac
     4141 ttaaagcaac aggtttggga agtatcccta ttggcgtgcg atgaatcgtt ctaataggaa
     4201 aatctttatt ttcatctaag atgactttga ataattcttt tacactcatc attcttcaat
     4261 cccgaataat tgttaccgca ctattttcta ttacacgtcc attggctttg tagccgtctg
     4321 ccgttttgac caaggtgctg ccgtggaagg agcaggtgta ggatttttga aaaacttaat
     4381 tcccaatatc ctgtttcaaa tgaaggaaat aggtcgt
//
LOCUS       DAWFHY010000116         4341 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.116, whole genome shotgun sequence.
ACCESSION   DAWFHY010000116 DAWFHY010000000
VERSION     DAWFHY010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.116"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            583..2469
                     /locus_tag="WGQ68_001930"
     CDS             583..2469
                     /locus_tag="WGQ68_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase component I
                     family protein/aminotransferase class IV"
                     /protein_id="HEZ0715669.1"
                     /translation="MPYFALFDDAVSGRAKRYQNHVESRFFRPEELDALDGALQSGWQ
                     KGLHSVLFADYGFGLPLTGVESERGGNLALHWFADCADTDAESWLARHSDGIPAGIST
                     PQSSVSETDYLDHIRQIHEAIRRGDTYQINYTTRLHLQAYGNPVSLYRRLRQPVPYAV
                     LSHLPDVEGQSAWTLCFSPELFLKIGSDGTISTEPMKGTAPILGDGQDERRAAELQTD
                     PKNRAENVMIVDLLRNDLGKIAQTGKVCVPEPFKVSRFGSVWQMTSTIQAQALPNTSF
                     ADILRAAFPCGSITGAPKKMSMQIIESLETEARGLYTGSIGYLNPCKSGLGFEGIFNV
                     VIRTLLLKPVSNSVSDDLDSRLNLNQDKATKPQTRQDEATPYRFKVNPLYQGVYGVGS
                     GIVIDSDPAAEYRECGWKARFLNELRPDFGIFETLRVENGRCTLLDRHLCRLKTSAQA
                     LNLPLPDGCENQIKQYIADLPDGAFRIKALLASDGISLSRAVLNRLTDKQRVIISPTI
                     LPAQNYLRRFKTTCRTVFDQAWQTAETQGAFDSLFFNSDGILLEGGRSNVFVKHRGQW
                     LTPSLDLDILNGIMRQAVLDEPQKYLQTNQVIETHITQKTLQEAEEIRLSNALRGVFA
                     AALA"
     gene            complement(2530..4047)
                     /locus_tag="WGQ68_001931"
     CDS             complement(2530..4047)
                     /locus_tag="WGQ68_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF560 domain-containing protein"
                     /protein_id="HEZ0715670.1"
                     /translation="MLYFRYGFLVVWCAAGVSAAYGADAPAILDDKALSQVWRSVSDK
                     WAESDWKVENDAPRVVDGDFLLAHPKMLEHSLRDALNGNQADLIASLADLYAKLPDYD
                     AVLYGRARALLAKLAGRPAEAVARYRKLHGENAADERILLDLAAAEFDDFRLKSAERH
                     FAEAEKLDLPAPVLENVGRFRKKAEGLTGWRFSGGISPAVNRNANNAAPQYCWQNGGR
                     QICSVSRAERAAGLNYEIEAEKLTALADNHYLLFRSNIGGTSYYFSKKSAYDDGFGRA
                     YLGWQYKNARQTAGILPFYQVQLSGSDGFDAKTKRVNNRRLSPYMLAHGVGVQLSHTY
                     RPNPGWQFSVALEHYRQRYREQDRAEYNNGRQDGFYVSSAKRLGESATVFGGWQFVRF
                     VPKRETVGGAVNNAAYRRNGVYAGWAQEWRQLGGLNSRVSASYARRNYKGVAAFSTEA
                     QRNREWNVSLALSHDKLSYKGIVPALNYRFGRTESNVPYAKRRNSEVFVSADWRF"
BASE COUNT         1087 a         1381 c          976 g          897 t
ORIGIN      
        1 aagtgggaat ctagaaataa aaagcaacag gcatttatcg gaaataactg aaaccgaaca
       61 gacctagatt cccgcctgcg cgggaatgac ggctgcagat gcccgacggt ctttatagcg
      121 gattaacaaa aatcaggaca aggcgacgaa gacgcagaca gtacagatag tggcgaggca
      181 acgctgtact ggtttttgtt aatccactat acttggagct gatcttgctt ttcgcctaat
      241 tctacgtttt caaacggttg cagctggtgg tctgccataa aggtctcctt attgtatttc
      301 aggttggaaa tgtttcaaca cacaggacga cacataaagc gtcgccctat gtgttgccct
      361 gatttggaag gggttacgcc cctcccaaat aaagtctgat tctaccgccc taaagggcgg
      421 ggtttaaacc gaaaaggaaa tacgatgaaa atccgttttc acaattttac accctcgccc
      481 cccactttct ctacataaaa ttacattttg ccgatatttg ccgaatcgtc tgaaaatatg
      541 tgtaaaaaga gcgtataatc aaaacatttg ccccgaattg ccatgcccta tttcgccctg
      601 tttgacgatg ccgtaagcgg tcgcgcaaaa cgctatcaaa atcatgtaga aagccgtttt
      661 ttccgtcccg aagaactcga tgctttggac ggcgcgctgc aatcgggctg gcaaaaaggg
      721 ctgcattcgg tgttgtttgc agactacgga ttcggtctgc cgctgacggg ggttgagtcc
      781 gaacgcggcg gcaatcttgc cctgcactgg tttgccgact gcgccgacac cgatgccgaa
      841 agctggcttg cccgacattc agacggcatc cccgccggca tttccacgcc gcaatcctcc
      901 gtatccgaaa ccgattacct cgaccacatc cgccaaatcc acgaagccat ccggcgcggc
      961 gacacctatc aaatcaacta caccacccgc ctgcacctgc aagcctacgg caatcccgtc
     1021 agcctctacc gccgcctgcg ccagcccgtc ccctatgccg tcttgtccca cctgcccgat
     1081 gtggaggggc aatccgcgtg gacgctgtgt ttctcgcccg aactcttcct caaaatcggt
     1141 tcggacggca ccatcagcac cgaaccgatg aaaggcactg cgccgatttt gggcgacgga
     1201 caagacgaac gccgcgccgc cgagttgcaa accgacccga aaaaccgcgc cgaaaacgtg
     1261 atgattgtcg atttgctgcg caacgacctc ggcaaaatcg cccaaaccgg caaagtatgc
     1321 gtgcccgagc cgtttaaagt atcgcgtttc ggcagcgtct ggcagatgac cagcaccatc
     1381 caagcccaag ccctgccaaa cacctcgttc gccgacatcc tccgcgccgc cttcccctgc
     1441 ggcagcatca ccggtgcgcc caaaaaaatg agtatgcaga ttatcgaatc gctcgaaacc
     1501 gaagcgcgcg gcctgtacac cggcagcatc ggctacctga atccatgtaa aagtgggctg
     1561 gggtttgaag gcatattcaa cgtcgttatc cgcaccttgt tgctcaaacc cgtttctaac
     1621 tcagtttcag acgacctaga tagtcgatta aatttgaatc aggacaaggc gacgaaaccg
     1681 cagacacggc aagacgaggc aacgccgtac cggtttaaag ttaatccact ctatcaaggg
     1741 gtgtacggtg tcggttccgg catcgtcatc gacagcgacc ccgccgccga atatcgcgaa
     1801 tgcggctgga aagcccgttt cctcaacgaa ttgcgccccg atttcggcat ttttgaaacc
     1861 ctgcgcgtcg aaaacggacg ctgcaccctg ctcgaccgcc acctatgccg tctgaaaacc
     1921 tccgcgcaag ccctcaacct gcccctgccc gacggttgcg aaaatcaaat caaacaatac
     1981 attgccgact tgcccgacgg cgcgttccgc atcaaagccc tgctcgcttc agacggcatc
     2041 agcctgtccc gcgccgtttt aaaccgtctg accgacaaac aacgcgtcat catttcgccg
     2101 accatcctgc ccgcacaaaa ctacctgcgc cgcttcaaaa ccacctgccg caccgtcttc
     2161 gaccaagcgt ggcaaaccgc cgaaacacaa ggcgcgttcg acagcctgtt tttcaattca
     2221 gacggcatcc tgctcgaagg cggcagaagc aacgtgttcg tcaaacatcg cggacaatgg
     2281 cttacgccct ctttagattt ggacatttta aacggcataa tgcgccaagc cgtattggac
     2341 gaaccgcaaa aatatttgca aacaaatcaa gtaatcgaaa cacacatcac acaaaaaaca
     2401 ctgcaagaag ccgaagaaat ccgcctctcc aacgccctgc gcggcgtatt tgccgccgcc
     2461 cttgcctgaa cgcgcaaaaa tgccgtccga acctgtttcc acaaggttca gacggcatta
     2521 ttccaccatt caaaaccgcc aatccgccga cacaaacacc tcgctgttgc ggcgtttcgc
     2581 atacggcaca ttactttccg tcctgccgaa acgataattc aacgccggta cgataccttt
     2641 gtacgacaac ttgtcgtggc tcaaagccag cgagacattc cattcgcggt tgcgttgcgc
     2701 ctctgtcgag aaagccgcaa cgcccttata gttgcggcgg gcataagacg cggaaacccg
     2761 actgttcaaa ccgcccaact gccgccactc ctgcgcccag ccggcataaa caccgttgcg
     2821 ccggtaggcg gcattattga ccgcgccgcc caccgtttcg cgtttcggca caaaccgcac
     2881 aaactgccag ccgccgaaca cagttgccga ttcgcccaaa cgttttgccg acgaaacata
     2941 aaacccgtcc tgccggccgt tattgtattc cgccctatcc tgttcgcggt agcgttggcg
     3001 gtaatgttcc agcgcgaccg aaaattgcca tcccgggttt gggcggtaag tatgggacag
     3061 ctgcacgccg actccgtgcg ccagcatata cggcgacagg cggcggttgt ttacccgttt
     3121 tgttttcgca tcaaagccgt cgctgcccga caactgcacc tgataaaacg gcaaaatccc
     3181 cgccgtctgc cgcgcatttt tatactgcca tcccaaatac gccctgccga acccgtcatc
     3241 ataagctgat tttttactga aataatagct cgtgccgccg atattggaac ggaacaacaa
     3301 ataatgattg tccgccaacg ccgtcagctt ttccgcctcg atttcataat tcaaccctgc
     3361 cgcccgctcc gcccggctga cactgcatat ctgccggcct ccgttttgcc agcaatactg
     3421 cggcgcggca ttattggcat ttctattgac cgccgggctg ataccgcccg aaaaacgcca
     3481 gcccgtcagc ccctccgctt ttttccgaaa acgccccaca ttttccaaaa ccggcgccgg
     3541 caaatccaat ttttccgcct cggcaaaatg cctttctgcc gacttcagcc ggaaatcgtc
     3601 aaactccgcc gccgccaaat ccagcaaaat ccgctcgtct gccgcatttt ccccgtgcag
     3661 cttccgatac cgcgccaccg cctccgctgg ccttcccgcc aatttcgcca gcaaagcccg
     3721 cgccctgccg tacaaaactg catcataatc cggcagcttg gcatacaaat ccgccaacga
     3781 agcgattaaa tccgcctgat tgccgttgag cgcgtcgcgc aaactatgtt ccaacatttt
     3841 cgggtgcgcc aacaaaaaat ccccgtcaac cacgcgcggg gcatcatttt caactttcca
     3901 atccgattcc gcccatttat ccgacaccga ccgccacacc tgcgacaaag ctttgtcatc
     3961 caaaattgca ggcgcatccg ccccataggc ggcagaaaca cctgccgcac accaaacaac
     4021 caaaaaaccg tatctgaaat acaacatacc ctgtcattta cctttccggc aaacacgccg
     4081 ccgaagcacg tcaaaccatc cgaaaaacag gcaaaagccc gtaaaaaccg gctttgccgc
     4141 ctgaaagcgg gcaggcaaaa aaccgccgcc cgattttcaa agggcggatt tcacatttat
     4201 agtggattaa caaaaatcag gacaagacga cgaagccgca gacagtacag atagtacgga
     4261 accgattcac ttggtgcttc agcaccttag agaattgttc tctttgagct aaggcgaggc
     4321 aacgccgtac cggtttttgt t
//
LOCUS       DAWFHY010000117         4169 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.117, whole genome shotgun sequence.
ACCESSION   DAWFHY010000117 DAWFHY010000000
VERSION     DAWFHY010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.117"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(66..2228)
                     /locus_tag="WGQ68_001932"
     CDS             complement(66..2228)
                     /locus_tag="WGQ68_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0715671.1"
                     /translation="MNAPFFRLSLLSLTLAAGFAHAAENNANVALDTVTVKGDRQGSK
                     IRTNIVTLQQKDESTATDMRELLKEEPSIDFGGGNGTSQFLTLRGMGQNSVDIKVDNA
                     YSDSQILYHQGRFIVDPALVKVVSVQKGAGSASAGIGATNGAIIAKTVDAQDLLKGLD
                     KNWGVRLNSGFASNEGVSYGASVFGKEGNFDGLFSYNRNDEKDYEAGKGFRNVNGGKT
                     VPYSALDKRSYLAKIGTTFGDGDHRIVLSHMKDQHRGIRTVREEFTVGSEDSRINIKR
                     QAPAYRETTQSNTNLAYTGKNLGFVEKLDANAYVLEKERYSADDSGTGYAGNVKGPNH
                     TRITTRGMNFNFDSRLAEQTLLKYGINYRHQEIKPQAFLNGEFSIPTKEKKNGKEVDK
                     PMEQQKKDRADEATVHAYKLSNPTKTDTGAYIEAIHEIDGFTLTGGLRYDRFKVKTHD
                     GKTVSNSNLNPSFGVIWQPHEHWSFSASHNYASRSPRLYDALQTHGKRGIISIADGTK
                     AERARNTEIGFNYNDGTFAANGSYFWQTIKDALANPQNRHVSAAVREAVNAGYIKNHG
                     YELGASYRTGGLTAKVGVSHSKPRFYDTHKDKLLSANPEFGAQVGRTWTASLAYRFKN
                     PNLEIGWRGRYVQKAEGSILVSGQKDRQGKLENVVRQGFGVNDIFANWKPLGKDTLNV
                     NLSVNNVFNTFYYPHSQRWTNTLPGVGRDVRLGVNYKF"
BASE COUNT          996 a          957 c         1125 g         1091 t
ORIGIN      
        1 atcaaattat cagaacagat gccgcctgaa aggctttcag acggcatttt ttcgggatat
       61 gcgttttaga acttgtagtt cacgcccaag cgtacatcgc ggcccacgcc cggcagggta
      121 ttggtccagc gttggctgtg cggatagtag aacgtgttaa acacgttgtt aaccgaaaga
      181 ttaacattga gcgtgtcttt gcccagcggt ttccagttgg cgaagatgtc gttcacaccg
      241 aaaccttggc gtacaacgtt ttccagtttg ccttggcggt ctttttgacc tgacaccaat
      301 atcgaaccct cagctttttg aacatagcgt ccgcgccagc cgatttccag attcgggttt
      361 ttgaagcggt aggcaaggga ggctgtccaa gtgcggccga cttgtgcgcc aaattcagga
      421 ttcgcgctca acagcttgtc tttgtgcgta tcgtaaaagc gcggtttgct gtggcttacg
      481 ccgactttgg cagtcaggcc gccggtgcgg taggacgcgc ccaattcgta accgtggttt
      541 ttgatgtaac cggcattgac ggcttcacgg acagcggcag agacgtggcg gttttgcgga
      601 ttggcaagcg cgtctttgat ggtttgccag aagtagctgc cgttggcggc aaacgtgccg
      661 tcgttgtagt tgaagccgat ttcggtattg cgcgcgcgtt cggctttcgt gccgtcggca
      721 atcgagatga tgccgcgttt accgtgggtt tgcagcgcgt catacaggcg cgggctgcgg
      781 ctggcgtagt tgtggctcgc gctgaagctc cagtgttcgt gcggctgcca aatcacgccg
      841 aaactcgggt taaggttgct gtttgaaacg gttttgccgt cgtgggtttt caccttgaag
      901 cggtcgtaac gcagcccgcc ggtcagggta aagccgtcaa tctcgtgaat ggcttcgata
      961 tacgcgccgg tatcggtttt ggtcggattg gaaagtttgt aggcgtgaac agtggcttca
     1021 tctgcacggt ctttcttttg ttgttccatc ggtttatcga cttctttacc gtttttcttt
     1081 tcttttgtcg ggatggagaa ttcgccgttc aaaaatgctt gcggtttgat ttcctgatgg
     1141 cggtagttga taccgtattt caacagggtt tgttcggcaa ggcggctgtc gaagttgaaa
     1201 ttcatacccc gagtggtgat tcgggtatgg ttggggcctt ttacattgcc tgcgtagccg
     1261 gtgccgctgt catcggcgga atagcgttct ttttccaaca cataggcgtt ggcatccagt
     1321 ttttcgacaa agcccaggtt tttacccgtg tacgccaaat tggtgttgga ttgtgtggtt
     1381 tcgcggtaag caggggcttg gcgtttaata tttatccgtg aatcttcgct gccgacggta
     1441 aattcttcac gcacagtgcg gatgccccgg tgttggtctt tcatatggct caacacgatg
     1501 cggtggtcgc cgtcgccgaa ggttgttccg attttggcga ggtagctgcg tttgtccagc
     1561 gcgctgtacg gtacggtttt gccgccgttg acattgcgga aacctttgcc ggcttcgtaa
     1621 tctttttcat cgttgcggtt gtaagagaac aagccgtcga agttgccctc ttttccgaat
     1681 acgctggcgc cgtagcttac gccttcgttg ctggcaaagc cgctgttgag gcgcacgccc
     1741 cagtttttat ccaagccttt gagcaggtct tgggcatcga cggttttggc gataatcgcg
     1801 ccgttggtcg cgccgatacc ggcagaggcg gaacccgcgc ctttttgtac ggaaacgact
     1861 ttaaccaaag cgggatcgac aataaatctg ccttggtggt aaaggatttg gctgtcggaa
     1921 taggcgttgt ccaccttgat gtcgacagag ttctgaccca tgccgcgcag cgtcaggaat
     1981 tgggacgtgc cgttgccgcc gccgaaatcg atggacggct cttcttttaa gagttcgcgc
     2041 atatcggttg cggtgctttc gtctttttgt tgaagcgtaa cgatgttggt acggattttg
     2101 ctgccttggc ggtcgccttt tacggtaacg gtatccaatg cgacattggc attattttct
     2161 gccgcatggg caaaacctgc cgccagggta agcgagagca ggctgaggcg gaaaaacggg
     2221 gcgttcattt gttcgtcctt ttgagtgtat gaagggaagt aaatccaaac cgttaaggtt
     2281 tggcaggata agaaaaataa taattatttt tgtttatatt aacgagaggg gggatttgtg
     2341 taaagctgat tatcgttttt atttgcgaag tgttgttttt tgttgacagg ttttgtcgga
     2401 aatgtatagt ggactaacaa aaatcaggat aaggcgacga agccgcagac agtacagata
     2461 gtacggcaag gcgaggcaac gctgtactgg tttttgttag tccactataa aaaacggcgg
     2521 aaatatagtg gattaacaaa tgcgggaatg acgaaaagca acctttccgc gtccttcccg
     2581 cgaaagcggg aatctagaac ccctaacgcg gcaggaatct atcggaaaaa accgaaaccg
     2641 aacgaaccta gattctcgcc tgcgcgggaa tgacgaagcc tgtgcgggaa tgacgaaaag
     2701 caacctttcc gcgtccttcc cgcgaaagcg ggaatctaga acctctaacg tggcaggaat
     2761 ctatcggaaa aaaccgaaac cgaacagact ggattcccgc ctgcgcggga atgacgaagc
     2821 ctgcgtggga atgacgaaaa gcaacttttc cgcgtccttc ccgcgaaagc gggaatctag
     2881 aacccctaac gcggcaggaa tctatcggaa aaaaccgaaa ccgaacgaac ctagattccc
     2941 gcctgcgcgg gaatgacgaa gcctgcgcgg gaatgacgaa aagcaacttt tccgcgtcat
     3001 tcccgcgaaa gcgggaatct aggaactcaa agctgcaaga atttatcaga aacaactgga
     3061 actcaaaaaa actagattcc cgcctgcgcg ggaatgacga agggttaaag aaatgatgag
     3121 aaacggcggt aatttatcgg gaataaccga aaacgaacgg aaatcaggac aaggcggcga
     3181 agccgcagac agtacggata ggacggaacc gattcacttg gtgcttcagc accttagaga
     3241 atcgttctct ttgagctaag gcgaggcaac gacgtactgg tttttgttca tccactataa
     3301 cagcaaccct gtcgccgtca ttcccgcgaa agcgggaatc cagtccgttc agtttcggtc
     3361 atttccgata aattcctgct gcttttcatt tctagattcc cacttttgcg ggaatgacgg
     3421 cggaagggtt ttggtttttt ccgataaatt cttgaggcat tgaaattcta gattcccgcc
     3481 tgcgcgggaa tgacggcgga gcggtttctg ttttttccga taaattccta aaacttaaaa
     3541 tttcatcatt ccctcaaaaa cagaaaacca aaaacagaaa cctaaaattc gtcattccca
     3601 cgaaagtggg aatccagaac gtaaaatctg aagaaaccgt tttatccgat aagtttccgc
     3661 accgacagac ctagattccc gcctgcgcgg gaatgacggc ggagcggttt ctgttttttc
     3721 cgataaattc ctaaaactta aaatttcatc attcccgcaa ggacagaaaa ccaaaaacag
     3781 aaacctaaaa ttcgtcattc ccacgaaagt gggaatctag aatcccggac tttcagataa
     3841 tctttgaata ttgctgttgt tctaaggtct agattcccgc ctgcgcggga atgacgatat
     3901 ttctgttttt gattttttgt ttttggggaa tgacgggatt tgagattgcg ggcatttatc
     3961 gggtaaaacg gaaattatgc gttacgaaaa tttatccgaa atcacggcaa cttttccacc
     4021 gtcattccca cgaaagtggg aatccaggtc tgtcggcacg gaaacttatc gagaaaaacg
     4081 gtttctttat agtggattaa caaaaatcag gacaaggcga cgaagccgca gacagtacgg
     4141 atagtacgga accgactcac tcggtgctt
//
LOCUS       DAWFHY010000118         4162 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.118, whole genome shotgun sequence.
ACCESSION   DAWFHY010000118 DAWFHY010000000
VERSION     DAWFHY010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.118"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(56..271)
                     /locus_tag="WGQ68_001933"
     CDS             complement(56..271)
                     /locus_tag="WGQ68_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715672.1"
                     /translation="MNTTYTLTFDQDSLKALNLLVFTLNHLKVLEMDMEGIEDGLSAV
                     IETASEKADKLSAAFYNAVYEQEEKAA"
     gene            complement(275..523)
                     /locus_tag="WGQ68_001934"
     CDS             complement(275..523)
                     /locus_tag="WGQ68_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEZ0715673.1"
                     /translation="MDRLKAAYLSARPDMVLLLRYTEMGLNQAEAAKLLGIAPSNVRA
                     RLKQLADLGLTEYRPDPKYRNRHALAAAHGQQSLGLEG"
     gene            complement(781..966)
                     /locus_tag="WGQ68_001935"
     CDS             complement(781..966)
                     /locus_tag="WGQ68_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715674.1"
                     /translation="MPDEKTLKAKQAEYQRKYDQKRLMKTVSFHLEREKELVDFMQKE
                     IPDFSNWVKSVMKEKMK"
     gene            complement(1239..1406)
                     /locus_tag="WGQ68_001936"
     CDS             complement(1239..1406)
                     /locus_tag="WGQ68_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715675.1"
                     /translation="MVDEKLAENRKRYEQKRVIKKVSFNAETEKELLEYAQNIDFSQW
                     VKSIIKEKIKK"
     gene            1929..2120
                     /locus_tag="WGQ68_001937"
     CDS             1929..2120
                     /locus_tag="WGQ68_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715676.1"
                     /translation="MNTVKEYTAVRERLLNAADYLEEVRKDRKTGNIASVEFVPPKIG
                     ARGYGKFKVRYKTLVAVDL"
     gene            2117..2533
                     /locus_tag="WGQ68_001938"
     CDS             2117..2533
                     /locus_tag="WGQ68_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715677.1"
                     /translation="MSGEKSENLPADEQQKMLQEFLELQKQDIAVKQKELESRKDEIQ
                     SHERIALATIEAQKQGEQQHGEIFKEVHKRRLNAVIAISFLIAAVLITALWRDKDSVA
                     IEIVKIGGAVALGYFAGINRGKAQTLEKQRREKDSD"
     gene            2710..3114
                     /locus_tag="WGQ68_001939"
     CDS             2710..3114
                     /locus_tag="WGQ68_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein GemA"
                     /protein_id="HEZ0715678.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCTECSIPEL
                     ERVVEDLRQHGFTPKKTAGRRPNRRSSADPMMRKIEALLLDNGWSWNYAQGTAKKMFK
                     VDRVEWLSDGNMHKLVAALQISANRKKKEKTG"
     gene            3111..3590
                     /locus_tag="WGQ68_001940"
     CDS             3111..3590
                     /locus_tag="WGQ68_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715679.1"
                     /translation="MSLNWEMTEQDFEAVKHLLPHSVVAMITVIGLEAAFHMVKVWGG
                     TNYPISNRRRNTRQSRILHAQLVEDIGEEAAGRLERAYVGQPFLAIPRCWDAMRELRN
                     RFIRRQYDAMSAEGLSDLFIVRELVLAHRLSARNIRYILKEADREAAARAQTDLFVA"
     gene            3753..>4162
                     /locus_tag="WGQ68_001941"
     CDS             3753..>4162
                     /locus_tag="WGQ68_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0715680.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVAAILRDDY
                     GLTVKTDGTGKGNMPLREAVKLIRGSDVAIEFHTNSAAGKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGRHSRLAYA"
BASE COUNT         1025 a          893 c         1101 g         1143 t
ORIGIN      
        1 cgtctgaaac ggggtttaaa acctgtttca gacggccttt tttcatgccc tagattcagg
       61 ctgctttctc ttcctgctcg tacacggcgt tgtagaaagc ggcggacagc ttgtcggctt
      121 tctcggaggc ggtttcaatc acggcagaca aaccgtcttc gattccttcc atgtccatct
      181 ccaaaacttt cagatggttg agtgtgaaca ccagcagatt cagggctttg aggctgtctt
      241 ggtcgaaagt cagggtatag gtggtattca tggcttaacc ctccaatccc aaagactgtt
      301 ggccgtgtgc cgccgccagg gcgtggcggt tgcggtattt cgggtcgggg cggtattcgg
      361 tcagacccag atcggcgagt tgtttgaggc gcgcgcgcac gttggacggc gcaatgccca
      421 agagtttggc ggcttccgcc tgattcagtc ccatttcggt gtagcgcagc aggagcacca
      481 tatcggggcg ggcggaaagg taggcggctt tgagcctgtc cattgcgctc tgtaaactct
      541 ttagagcatt ccaaattttg aatggcgcgt aacacatctt tgtgcttctt gccgaaattg
      601 tttgatacgg cgagcgaggt tgtaaccgga cggtcgccgg agatttgaac aagttgggtg
      661 ttgttggtat tcatgataga atagcctctc ttttagattg catttaaaag acacgagaaa
      721 gaacgttgca gcgttgcttt cccactgttc ccccgatgtt ccaccatcgg gggaattttc
      781 ttatttcatt ttttccttca tgactgattt aacccagttt gaaaagtcgg gaatttcttt
      841 ttgcataaaa tccaccaact ctttctctct ttccaaatgg aaagaaactg ttttcatcag
      901 ccgcttttgg tcatactttc gctgatattc agcttgtttt gcttttagcg ttttttcatc
      961 aggcataggc aatccttgat ttttaaaatc gttgtagtaa aatggcttgc agaaaggttg
     1021 gcgaaagggg cgcggtgttc tgccacccct tgtgtttaat caacttgctt agtaagccgg
     1081 actacagctt aaaagcagaa tgatgaaaaa aacaactttc gccagttttg tcatttccac
     1141 aagcctcctt tctatccgat aagcccccga agttgccgct tcggggcttt gctttatcag
     1201 ataggcgtat tatctagtat ttctagaatt tagtcaagct attttttgat tttttctttg
     1261 atgattgatt taacccattg ggaaaaatcg atattttggg catattccaa aagttctttt
     1321 tccgtttcgg cattgaacga gactttctta atcacccgtt tctgctcgta acgctttcgg
     1381 ttttctgcca gtttttcatc aaccataggc aaactccttg attttttaaa ccgtattttg
     1441 taagattggg actggggaca gcggcgaccg cccccagctg gtcagtcttt agtaggcatt
     1501 agcacttgcc aacatgaaaa ctgcgaccaa gatgagaatt tgaatagtgc gtttattcat
     1561 cttctttctc ccagttgagc cccgcttcgg tggggctttt cccgtatcgg ccctcactac
     1621 gccgatgaat tgaattataa acgtggacac gttaaaagtc aaccctttcc ccaacaaaat
     1681 caaagaaaaa tgccgtctga aaacagctcg aatgctgttt cagacggcat tttttgttgt
     1741 cgggcatcaa tgccctgcca tttgtgcggc gttgcggtta tcaagatatg gtattttagt
     1801 gtgtattttt agaagaaatt taccatatat agtattaaaa tattatacgg ttgttttata
     1861 tggatttgat tttcttgaaa aataaatccc atatgctaat atttgcacta tttgggcgag
     1921 gttgaaaaat gaataccgtc aaagaatata cggcagtccg cgaacggctg ctcaatgctg
     1981 ccgattattt ggaagaagtc cgtaaagacc gtaagacggg caatattgcg tccgtggaat
     2041 ttgtgccgcc gaaaataggg gctcggggtt acggcaaatt caaagtccgt tacaaaacct
     2101 tagtggcggt tgatttatga gcggggaaaa atccgagaat ctgccggccg atgagcagca
     2161 gaaaatgctt caggagtttt tagaacttca aaaacaggat attgcagtca aacagaagga
     2221 gttggaaagc cgtaaggacg aaatccaatc tcacgaacgc attgcactgg ctaccatcga
     2281 agcgcaaaaa cagggtgaac agcaacatgg tgagattttc aaagaggtac acaaacgccg
     2341 tctgaatgcc gtgattgcta tttcttttct gattgccgcc gtgctgatta ctgctttatg
     2401 gcgcgataag gacagcgtcg ctattgaaat tgtcaaaatc ggcggggcgg tagctttggg
     2461 atattttgca ggaatcaaca gaggtaaagc gcaaaccttg gaaaagcaac ggcgcgaaaa
     2521 agacagtgat tagattgtag taaaaaaggc cgtctgaaat gttttggcga aactcgcaaa
     2581 gctcgttttc agacggcttt ttcatgcctg tccgtttcgt aaaaaaaaca gcggcttact
     2641 acaatatata gtattttata tgtataatat gcatcaatta atcaatatat tgtgttttat
     2701 gggtttgaaa tgcgccgggc gttgattgcg aaaattaaga ttgctcaaaa ggagctgggc
     2761 ttggatgacg gtacctatcg agcggtgtta gagcgggtga cgggcaagcg gtcgtgtacg
     2821 gagtgcagta tccctgagct ggaacgcgtg gtcgaggatt tgcgccaaca tggttttacg
     2881 ccgaaaaaaa cggcaggccg acggccaaac cgccgctctt ctgccgaccc gatgatgcgc
     2941 aaaatcgaag ccctgctgct ggataacggc tggtcttgga attatgcgca gggtacggcg
     3001 aaaaagatgt ttaaggttga ccgcgtggaa tggttgtccg acggcaatat gcacaagttg
     3061 gtggcggctt tgcagattag cgcgaaccgc aagaaaaagg agaaaacggg atgagtttga
     3121 actgggagat gacagagcag gactttgagg ctgtgaaaca tctgctgccg cacagtgtgg
     3181 tggcgatgat tacggttatc gggctggagg cggcgtttca catggttaag gtttggggcg
     3241 ggacgaatta tccgatttcc aaccgccgcc gcaatacgcg tcagagccgc atcttacacg
     3301 ctcaactggt cgaggacatc ggcgaagaag cagcgggacg gttggagcgt gcttatgtcg
     3361 ggcagccttt cttggcgatt ccgcgctgct gggatgcgat gcgcgaactt cgcaaccggt
     3421 tcatccgccg ccaatatgat gcgatgagcg cggaaggttt gagcgatttg tttattgtgc
     3481 gcgagctggt attggctcat cggctgtctg cgcgcaatat ccgatacatt ttgaaagagg
     3541 ccgaccgcga ggcggcggca agagcgcaga cggatttatt tgtagcttga ttgtgttgtg
     3601 tgtttcttgt gagtggacct ttgccctgct ttttgcaggg cttttttttg tctgcggaac
     3661 agtaagtgca tttgtgccga ctgtcttatg ccgtctgaaa aggttaaata aggttttgaa
     3721 aataaattgt gatttgattt tcggagatgt ttatgggcaa aactgtaact ttaaccgccg
     3781 gccacagcaa caccgacccg ggtgcggtca acggcagcga ccgtgaggcg gacttggcgc
     3841 aggatatgcg caacatcgtg gctgctattt tgcgcgatga ctacggtttg actgttaaaa
     3901 ccgacggcac aggcaaaggc aatatgccgc tgcgtgaagc tgtaaaactg attcgcggct
     3961 cggatgtggc gattgagttc cataccaact cggcggccgg taaagcagct acgggcattg
     4021 aggcgttgag taccgtcaaa aacaaacgct ggtgtcaggt gttgagcaaa gccgttgcca
     4081 agaaaaccgg ctggaaactg cgcggcgaag acggctttaa acccgacaat gcgggccggc
     4141 attcgcgcct ggcttatgcg ca
//
LOCUS       DAWFHY010000119         4162 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.119, whole genome shotgun sequence.
ACCESSION   DAWFHY010000119 DAWFHY010000000
VERSION     DAWFHY010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4162)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4162)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4162
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.119"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(208..1458)
                     /locus_tag="WGQ68_001942"
     CDS             complement(208..1458)
                     /locus_tag="WGQ68_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEZ0715681.1"
                     /translation="MFSKSVTLAQYDPDLAAAIAQEDQRQQDHVELIASENYVSCAVM
                     DAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRVKKLFGAQYANVQPHSGSQAN
                     QAVYASVLKPGDTILGMSLAHGGHLTHGASVNISGKLYNAVTYGLDENEVLDYAEVER
                     LALEHKPKMIVAGASAYALQIDWAKFREIADKVGAYLFVDMAHYAGLVAGGEYPNPVP
                     FCDFVTTTTHKTLRGPRGGVILCRDNTHEKALNSSIFPSLQGGPLMHVIAAKAVAFKE
                     ALQPEFKQYAKQVKINAAAMAEELVKRGLRIVSGRTESHVFLVDLQPMKITGKAAEAA
                     LGKAHITVNKNAIPNDPEKPFVTSGIRIGSAAMTTRGFNEADVRVLANLVADVLSNPE
                     DEANLAKVREQVTALCNKYPVYGA"
     gene            complement(1524..1736)
                     /locus_tag="WGQ68_001943"
     CDS             complement(1524..1736)
                     /locus_tag="WGQ68_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715682.1"
                     /translation="MADKDVVGAGYLIDFAQTAENLPFNVLLLISLVLNKGDETLKTS
                     MLNKLPMLNKLPDNAKENLRIMGYLP"
     gene            complement(1957..3732)
                     /gene="ggt"
                     /locus_tag="WGQ68_001944"
     CDS             complement(1957..3732)
                     /gene="ggt"
                     /locus_tag="WGQ68_001944"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEZ0715683.1"
                     /translation="MAKTYLLTALIMSMTISGCQVIHANQGKVNTHSAVITGADAHTP
                     EHATGLTEQKQVIASDFMVASANPLATQAGYDILKQGGSAADAMVAVQTTLSLVEPQS
                     SGLGGGAFVLYWDNTAKTLTTFDGRETAPMRATPELFLDKDGQPLKFMEAVVGGRSVG
                     TPAIPKLMETIHQRYGVLPWGKLFDTPIRLAKQGFEVSPRLAISVEQNQQHLARYPKT
                     AAYFLPNGVPLQAGSLLKNLEFADSVQALAAQGAKALHTGKYAQNIVSVVQNAKDNPG
                     QLSLQDLSDYQVVERPPVCVTYRIYEVCGMGAPSSGGIAVGQILGILNEFSPNQVGYD
                     AEGLRLLGDASRLAFADRDVYLGDPDFVPVPIRQLISKDYLKHRSQLLEQSDKALPSV
                     SAGDFIHEWVSSQAIELPSTSHISIVDKAGNVLSMTTSIENAFGSTLMANGYLLNNEL
                     TDFSFEPIKQGKQVANRVEPGKRPRSSMAPTIVFKAGKPYMAIGSPGGSRIIGYVAKT
                     IVAHSDWNMDIQDAISAPNLLNRFGNYELETGTTALQWQQALNDLGYKTDVRELNSGV
                     QAIIIEPSRLVGGADPRREGRVMGD"
     gene            complement(3900..4162)
                     /locus_tag="WGQ68_001945"
                     /pseudo
     CDS             complement(3900..4162)
                     /locus_tag="WGQ68_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007116440.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin, Grx4 family"
BASE COUNT         1048 a          972 c          983 g         1159 t
ORIGIN      
        1 tggtccaact tcaatagtgg gaaacggtcg atgtgtttgg caatcatggc ttgggcggtt
       61 tgctggaaga aggtgctcat ggaaaatccc ctaaatggct tggtgggaat ttaggggatt
      121 tggggggatt ttgcaaaggt ttccgcctga aacattatga gatttcaggc ggcattggat
      181 tgcttggcgg aatattttta aaaaggctta cgcgccgtaa acggggtatt tattgcacaa
      241 agcagttact tgttcgcgga ctttggcgag gttggcttcg tcttcggggt tagacaatac
      301 gtcggcaacc aagttcgcca atacgcgcac gtcggcttcg ttaaaaccgc gtgtggtcat
      361 ggcggcggaa ccgatgcgga tgccggaggt aacgaagggt ttttccggat cattcgggat
      421 ggcgtttttg ttgacagtga tgtgcgcttt gcccaaagca gcttcggcgg ctttgccggt
      481 aattttcatc ggttgcaggt caacgaggaa aacgtggctt tcggtgcggc cggaaacgat
      541 gcgcaaaccg cgtttaacca actcttccgc catggcggct gcattgattt tcacttgttt
      601 tgcgtattgt ttgaactcgg gttgcaatgc ttctttaaac gccacggctt tggcggcgat
      661 aacgtgcatc agcgggccgc cttgcaggct tgggaagatg gaagagttca gtgctttttc
      721 gtgtgtgttg tcgcggcaca aaattacgcc gccgcgagga ccgcgcaggg ttttgtgggt
      781 ggtggtggta acgaagtcgc agaacggcac gggattggga tattcgccgc cggcaaccag
      841 accggcatag tgcgccatat cgacaaagag gtatgcgccg actttatcgg cgatttcgcg
      901 gaattttgcc cagtcgattt gcaacgcgta ggcagacgca cccgccacaa tcattttggg
      961 tttgtgttcg agcgcgaggc gttcgacttc ggcataatcg agcacttcgt tttcatccaa
     1021 accataagta acggcattgt agagtttgcc tgagatatta acgctcgcgc cgtgggtcag
     1081 gtggccgccg tgcgccagag acatacccaa aatggtgtcg cctggtttta aaacggaagc
     1141 gtacacggct tggttggctt gcgagccgga gtgcggttgg acgttggcgt attgcgcgcc
     1201 gaacagtttt ttgacgcggt caatcgccaa ttgttcgaca atatcgacgt attcgcagcc
     1261 gccgtagtag cgtttgccag gatagccttc ggcgtatttg ttggtcaatt gcgaaccttg
     1321 cgcgtccatt acggcgcagc taacgtagtt ttcggaagca atcagctcga cgtggtcttg
     1381 ctggcgttgg tcttcttggg caatggctgc tgccaaatcg gggtcgtatt gtgcgagggt
     1441 aacgcttttt gaaaacatgt tctcggctcc tttgtgtaat cagggtatca tgagtgtttt
     1501 ttgtataaaa aatacttcaa tacctaaggt agatagccca taatgcgtaa attttctttg
     1561 gcattatcag gtaatttatt taacatgggt aatttattta acatgctggt ttttagcgtc
     1621 tcatcacctt tatttagtac aagactaatc aaaagcaata cattaaatgg taaattttcg
     1681 gcagtttgtg caaaatcaat caaataacca gcaccaacca catctttatc agccataacc
     1741 cgctgataca ccacttgcta agacaactct cctaccccac catgtcttaa aatcagatga
     1801 atggcttgta atgctgtgtt tggtgtctca caaattgcca aaatctgatc agaagcttgc
     1861 ttgatggctt gtatttttgt ttgattactc acaatttccc cctattttaa taattaactt
     1921 aaatgcggtc aaattcacaa aatacaagct tcacctctaa tcacccatca ctcgaccttc
     1981 tcggcgtgga tcggcaccac caaccagcct gcttggctcg ataataatgg cttgaacacc
     2041 tgaatttagc tcacgcacat cagtcttata gcccaaatca tttaatgctt gttgccactg
     2101 gagggcggtt gtgcccgttt ctagttcata gttaccaaag cgatttaata aattgggtgc
     2161 actgatggca tcttggatat ccatattcca gtcactatgt gccacaatcg tcttagcgac
     2221 atagccaatg atacggctac cacctgggga gccgattgcc atataaggct tgcctgcttt
     2281 aaatacgatg gttggtgcca ttgaggagcg tggtctcttg ccgggctcga cacgattggc
     2341 gacctgtttg ccctgcttta ttggctcaaa actaaagtct gtcagctcat tattcagcag
     2401 gtagccattt gccatcaaag ttgagccaaa cgcattttca atggaagtcg tcattgatag
     2461 cacattgccc gccttatcca caattgatat atgactggta gaaggtaact caatcgcttg
     2521 tgaggacacc cactcatgaa taaaatcgcc tgcagatacg ctaggcaatg ccttatccga
     2581 ctgctcaagc agctggctgc gatgttttag gtagtcttta gaaatcaact ggcgaatggg
     2641 tactggtaca aaatcagggt cgcccaaata tacatcacga tccgcaaacg caagcctaga
     2701 agcgtcgccc aagagacgta aaccttcagc atcatacccc acctgattgg gtgaaaattc
     2761 atttaaaatc cccaaaatct gacccacagc aatcccacct gagcttggtg cacccatacc
     2821 gcatacttca taaatacgat aagtcacaca aacaggcggg cgttccacca cttgataatc
     2881 agataaatct tgtaaggata attgaccggg gttatcctta gcattttgga caactgaaac
     2941 gatattttgg gcatatttac cagtatgcag agcttttgca ccttgagctg ctaacgcctg
     3001 aacactgtca gcaaattcta aatttttcag caagctgcct gcttgtagcg gcacaccatt
     3061 cggcaaaaaa taagcggctg tttttggata gcgtgccaaa tgctgctgat tttgctcaac
     3121 cgagatggca agccttggcg acacctcaaa gccttgtttt gccaagcgga tcggcgtatc
     3181 aaataatttt ccccaaggca atacaccgta tcgctgatgt attgtctcca tcagtttagg
     3241 gatagcaggc gtacccaccg agcgaccacc gaccaccgct tccataaatt tcaatggttg
     3301 accatcttta tccaaaaata attccggcgt cgcacgcatc ggtgccgtct cacgcccatc
     3361 aaatgtggtc aatgttttgg cggtattatc ccaatacaac acaaatgctc caccgcccaa
     3421 gcctgacgac tgtggctcta ccaagcttag tgtcgtctgc accgccacca tcgcatctgc
     3481 agcgctaccg ccttgcttta agatatcata gccagcttgt gttgctaatg gattggctga
     3541 cgctaccata aaatcacttg caatcacctg cttttgttcg gtcagtcccg ttgcatgttc
     3601 aggcgtgtga gcgtctgcac ctgtgatgac agcagaatga gtattaacct taccttgatt
     3661 ggcatggatg acttgacatc cagagattgt catagacatt atcaatgcag tcaataaata
     3721 tgttttagcc acaagcactc cttcgcctga gtttgattga tgattcatac aaggcatgct
     3781 gattattgta tttaatatgg ctaaataatt caatccaaac tatcaatctt gaccatcaaa
     3841 aaaagaccgc taatgtcatc agcagtcttt tttgatattt attttaagat attaagtaat
     3901 cagacctttg ggctatgctc ttcaatgagt ggttttagct cacctgattg gtacatttgt
     3961 aggataatat cactaccacc gattaactca ccattaaccc aaagctgtgg aaaggttggc
     4021 cgattggcga tgagtggtag agtactgcga atttctgggt tttctaggat attaacaaaa
     4081 gcaaagggtc tgccaattgg gtcagcacct ctactgcacg cgctgaaaat ccacattggg
     4141 gaaactgggg cgtgcctttc at
//
LOCUS       DAWFHY010000120         4129 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.120, whole genome shotgun sequence.
ACCESSION   DAWFHY010000120 DAWFHY010000000
VERSION     DAWFHY010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.120"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            43..675
                     /locus_tag="WGQ68_001946"
     CDS             43..675
                     /locus_tag="WGQ68_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEZ0715684.1"
                     /translation="MENTNRVPEQARYDAERRQADEALAGVFPAVSIFGSARTPQNHA
                     DYAFACRLARRLSDSGIAVISGGGPGIMEAANKGAFAGKSVSVGLNIVLPHEQKPNPY
                     QDIALRFSRFAERKAVFFRYSQAYVVMPGGFGTLDELFEILTLVQTGKVPPRPIVLVG
                     KAFWSGLAEWINAQLLARGLISEGAVSLFAISDDEDEIVAYLSEHGLQTA"
     gene            747..2117
                     /gene="purB"
                     /locus_tag="WGQ68_001947"
     CDS             747..2117
                     /gene="purB"
                     /locus_tag="WGQ68_001947"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEZ0715685.1"
                     /translation="MINPIASLSPLDGRYAQSVEALRPIFSEYGLMKARVKVELNWLK
                     ALAAEPKIVEVPPFSAETLAEIDTVIENFSLEDAAAVKAIEATTNHDVKAIEYWLKKR
                     FAEVPEVAAVSEFVHFACTSEDINNLSHALMLQEAREAVLLPKLAEIIEKLTAMAHDL
                     AAVPMMSRTHGQPATPTTLGKETANVVYRLQRQFKNLQAQEFLGKINGAVGNYNAHMV
                     AYPDVDWETHCRNFVEISLGLTFNPYTIQIEPHDYMAEFFQTLSRINTILIDFNRDVW
                     GYISLGYFKQKVKAGEVGSSTMPHKVNPIDFENSEGNLGMANAVLGFLSEKLPISRWQ
                     RDLTDSTVLRNMGVGVGYAVLGFAAHLRGLNKLEPNPAALAADLDATWELLAEPIQTV
                     MRRYGVANPYEKLKDLTRGKGGITPEVLKGFIGSLEIPVEAKAKLLELTPALYVGKAE
                     ALAKRI"
     gene            2197..4065
                     /locus_tag="WGQ68_001948"
     CDS             2197..4065
                     /locus_tag="WGQ68_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002241050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEZ0715686.1"
                     /translation="MQAVRYRPEIDGLRAVAVLSVIIFHLNNRWLPGGFLGVDIFFVI
                     SGFLITGIILSEIQNGSFSFRDFYTRRIKRIYPAFIAAVSLASVIASQIFLYEDFNQM
                     RKTVELSAVFLSNIYLGFQQGYFDLSADENPVLHIWSLAVEEQYYLLYPLLLIFCCKK
                     TKSLRVLRNISIILFLILTATSFLPSGFYTDILNQPNTYYLSTLRFPELLAGSLLAVY
                     GQTQNGRRQTANGKRQLLSSLCFGALLACLFVIDKHNPFIPGMTLLLPCLLTALLIRS
                     MQYGTLPTRILSASPIVFVGKISYSLYLYHWIFIAFAHYITGDKQLGLPAVSAVAALT
                     AGFSLLSYYLIEQPLRKRKMTFKKAFFCLYLAPSLILVGYNLYARGILKQEHLRPLPG
                     APLAAENHFPETVLTLGDSHAGHLRGFLDYVGSREGWKAKILSLDSECLVWVDEKLAD
                     NPLCRKYRDEVEKAEAVFIAQFYDLRMGGQPVPRFEAQSFLIPGFPARFRETVKRIAA
                     VKPVYVFANNTSISRSPLREEKLKRFAANQYLRPIQAMGDIGKSNQAVFDLIKDIPNV
                     HWVDAQKYLPKNTVEIYGRYLYGDQDHLTYFGSYYMGREFHKHESLLKHSHGNALQ"
BASE COUNT          948 a         1113 c         1063 g         1005 t
ORIGIN      
        1 tttttgacgg tgtacaatcg ctgtttttga acggaggatg gaatggagaa tacaaaccgt
       61 gtgccggagc aggcacgtta tgatgccgaa cgcaggcagg cagacgaagc attggcgggc
      121 gtgtttccgg cagtcagtat cttcggcagc gcgcgcacgc cgcagaatca tgcggattat
      181 gcgttcgcct gccgtctggc gcggcggctg tcggattcgg gcattgccgt catttcgggc
      241 ggcgggccgg ggattatgga ggcggcaaac aagggcgcgt ttgcagggaa gtcggtttcg
      301 gtggggctga acatcgtttt gccgcacgag cagaaaccga atccgtatca ggacatcgcc
      361 ttgcggtttt cccgttttgc cgaacgcaag gcggtgtttt tccgctattc ccaagcatat
      421 gtcgtgatgc cgggcggctt cgggacgctg gacgaattgt ttgaaatcct gaccttggtg
      481 cagacgggca aagtgccgcc gcgtccgatt gttttggtcg gaaaggcgtt ttggtcgggc
      541 ttggcggagt ggataaacgc gcagcttttg gcgcgcgggc tgatttccga aggggcggtc
      601 tctttgtttg ccatatcgga cgatgaagac gaaatcgttg cgtatctgtc ggaacacggg
      661 cttcagacgg catagcgtcc tgagagtgat gtataattgc aaacaattta acaatttttg
      721 atgtctttcc cgaacaggat gccgaaatga tcaaccccat cgcctcgctt tcccctttag
      781 acggccgtta tgcccaatcc gttgaagcat tgcgcccgat tttttcagag tacggcctga
      841 tgaaggcgcg cgtcaaagtc gaattaaact ggctcaaagc cctggccgcc gagccgaaga
      901 ttgtcgaagt gccgcccttc agtgccgaaa cgcttgccga aatcgacacg gtgattgaaa
      961 acttttcatt ggaagacgca gccgctgtca aagccatcga agccacgacc aatcatgatg
     1021 tcaaagccat tgaatattgg ctgaaaaaac gttttgccga agtgccggaa gtcgccgccg
     1081 tgagtgagtt cgtccacttc gcctgcacca gcgaagacat caacaacctg tcccacgctt
     1141 taatgctgca agaagcgcgt gaggctgttt tgctgccgaa gctggccgaa atcatcgaaa
     1201 aactgaccgc tatggcgcac gaccttgccg ccgtcccgat gatgagccgc acccacggcc
     1261 agcccgccac gccgaccact ttgggcaaag aaaccgccaa tgtcgtgtac cgcctgcaac
     1321 gccagtttaa aaacctgcaa gcgcaagagt tcctcggcaa aatcaacggc gcggtcggca
     1381 actacaacgc ccatatggtc gcctatcctg atgtagattg ggaaacccac tgccgcaact
     1441 tcgtcgaaat cagcctcggt ctgaccttca acccctacac catccaaatc gaaccgcacg
     1501 actatatggc ggaattcttc caaaccctca gccgcatcaa cacgattctc atcgacttta
     1561 accgcgacgt ttggggttat atttcattgg gttacttcaa acaaaaagtc aaagcaggcg
     1621 aagtcggttc ttccaccatg ccgcacaaag tcaaccccat cgactttgaa aactccgagg
     1681 gcaacctcgg tatggcaaac gccgtattgg gctttttgtc cgaaaaactg ccgatttccc
     1741 gctggcagcg cgacctgacc gacagcaccg tcttacgcaa tatgggcgta ggcgtgggct
     1801 atgccgtatt gggtttcgcc gcccacctgc gcggtctgaa caagctcgaa cccaaccccg
     1861 ccgcgcttgc cgccgatttg gatgccactt gggaactgct cgccgagccg attcaaaccg
     1921 tgatgcgccg ttacggtgtc gccaatcctt acgaaaaact gaaagacctg acgcgcggca
     1981 aaggcggcat tacgcccgaa gtgctgaaag gctttatcgg atcgctggaa atccccgtcg
     2041 aagccaaagc aaaattgctt gagctgaccc ccgcactgta tgtgggcaag gctgaagcgt
     2101 tggcgaaacg gatttgagcg tttactgaaa ccgatgccgt ctgaacgcgc gttcagacgg
     2161 catttttaag ataacgggac atacggggcg atatttatgc aagctgtccg atacaggcct
     2221 gaaattgacg gattgcgggc cgttgccgtg ctatccgtca ttattttcca cctgaataac
     2281 cgctggctgc ccggagggtt tttgggggtg gacattttct ttgtcatctc gggattcctc
     2341 attaccggca tcattctttc tgaaatacag aacggttctt tttctttccg ggatttttat
     2401 acccgcagga ttaagcggat ttatcctgct tttattgcgg ccgtgtcgct ggcttcggtg
     2461 attgcctctc aaatcttcct ttacgaagat ttcaaccaaa tgcggaaaac cgtggagctt
     2521 tctgcggttt tcttgtccaa tatttatctg gggtttcagc aggggtattt cgatttgagt
     2581 gccgacgaga accccgtact gcatatctgg tctttggcag tagaggaaca gtattacctc
     2641 ctgtatcctc ttttgctgat attttgctgc aaaaaaacaa aatcgctacg ggtgctgcgt
     2701 aacatcagca tcatcctatt tctgattttg actgccacat cgtttttgcc aagcgggttt
     2761 tataccgata ttctcaacca acccaatact tattaccttt cgacactgag gtttcccgag
     2821 ctgttggcag gttcgctgct ggcggtttac gggcaaacgc aaaacggcag acggcaaaca
     2881 gcaaatggaa aacggcagtt gctttcatca ctctgcttcg gcgcattgct tgcctgcctg
     2941 ttcgtgattg acaaacacaa tccgtttatc ccgggaatga ccctgctcct tccctgcctg
     3001 ctgacggcac tgcttatccg gagtatgcaa tacgggacac ttccgacccg catcctgtcg
     3061 gcaagcccca tcgtatttgt cggcaaaatc tcttattccc tatacctgta ccattggatt
     3121 tttattgcct tcgcccatta cattacaggc gacaaacagc tcggactgcc tgccgtatcg
     3181 gcggttgccg cgttgacggc cggattttcc ctgttgagtt attatttgat tgaacagccg
     3241 cttagaaaac ggaagatgac cttcaaaaag gcatttttct gcctctatct cgccccgtcc
     3301 ctgatacttg tcggttacaa cctgtacgca agggggatat tgaaacagga acacctccgc
     3361 ccgttgcccg gcgcgcccct tgctgcagaa aatcattttc cggaaaccgt cctgaccctc
     3421 ggcgactcgc acgccggaca cctgcggggt tttctggatt atgtcggcag ccgggaaggg
     3481 tggaaagcca aaatcctgtc cctcgattcg gagtgtttgg tttgggtaga tgagaagctg
     3541 gcagacaacc cgttatgtcg aaaataccgg gatgaagttg aaaaagccga agccgttttc
     3601 attgcccaat tctatgattt gaggatgggc ggccagcccg tgccgagatt tgaagcgcaa
     3661 tccttcctaa tacccgggtt cccagcccga ttcagggaaa ccgtcaaaag gatagccgcc
     3721 gtcaaacccg tctatgtttt tgcaaacaac acatcaatca gccgttcgcc cctgagggag
     3781 gaaaaattga aaagatttgc cgcaaaccaa tatctccgcc ccattcaggc tatgggcgac
     3841 atcggcaaga gcaatcaggc ggtctttgat ttgattaaag atattcccaa tgtgcattgg
     3901 gtggacgcac aaaaatacct gcccaaaaac acggtcgaaa tatacggccg ctatctttac
     3961 ggcgaccaag accacctgac ctatttcggt tcttattata tggggcggga atttcacaaa
     4021 catgaaagct tgctcaagca ttcacacggc aacgcattgc agtagcctgc cttcttgtcg
     4081 gatattgcct ttggcagcct atgccgctgt ttgcccttcg gggcggcgg
//
LOCUS       DAWFHY010000121         4024 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.121, whole genome shotgun sequence.
ACCESSION   DAWFHY010000121 DAWFHY010000000
VERSION     DAWFHY010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.121"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(1..892)
                     /locus_tag="WGQ68_001949"
                     /pseudo
     CDS             complement(1..892)
                     /locus_tag="WGQ68_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            complement(1097..1405)
                     /locus_tag="WGQ68_001950"
     CDS             complement(1097..1405)
                     /locus_tag="WGQ68_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003678109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715687.1"
                     /translation="MDVFPELNIHCFYDDKNQLEAIEIFEPNKFFLEDISFNWLGKIN
                     DEIISYLYKSGINDFEIDDLGIRLLNIPVSTIINSIGKVECCYLDFSNRDTKFYVKQV
                     "
     gene            complement(1856..2179)
                     /locus_tag="WGQ68_001951"
     CDS             complement(1856..2179)
                     /locus_tag="WGQ68_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715688.1"
                     /translation="MSILEENVIDSASIENGILILTISDHLKWDNEHLFLLQEKINSY
                     IQYIGSGQIFEDFGESSYETIEIQLIYKYKPNENYRKFLYRLEGALLKLKLRFSHGTI
                     SYFYS"
     gene            complement(2334..2443)
                     /locus_tag="WGQ68_001952"
                     /pseudo
     CDS             complement(2334..2443)
                     /locus_tag="WGQ68_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225556.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
     gene            complement(2475..2936)
                     /locus_tag="WGQ68_001953"
     CDS             complement(2475..2936)
                     /locus_tag="WGQ68_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715689.1"
                     /translation="MNIIMDSTRKFGILWEKNSECNGFIYGKIQIIIGENIYPKICPY
                     GYFTLNTVFNSLKSSFEEKYYAGGNNGLDFGEQLFDIDKYNSLELYNIFSIDTAYMSG
                     GSNCEIDCLVLEMGYSSEEERLFYSFDNGKNFKEIRYKKGTVESVIFQLNL"
     gene            complement(2945..3862)
                     /locus_tag="WGQ68_001954"
     CDS             complement(2945..3862)
                     /locus_tag="WGQ68_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003712141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715690.1"
                     /translation="MRIRRQILRWVDKGSQDGYTDQTVISTIGMKGEDKALGYTWDYR
                     NYGARNPQAYNDPKLFEEYRRQDKPEYRNLTWLHSGTKDTKIRQGERKNEEFALNVAD
                     GLTSLVNPNPRIKAPILAAIRNLGNIKPTVTGSDPLLAGAGNIRIPANGNVAKGDRIP
                     DTALASKSHQKNDFGTSKGQGAESSIPVPQAISIKIKNPDKTVSETTYQSNPKHTPGQ
                     LGFKFDAGIEPKNSVQLIESSVQIGKQRFAIDDKGHIHRYMGGDRKNEPWHWAGSTSD
                     KKNPLSLTSQQKAGIKRAYPEQSKNKLLK"
BASE COUNT          995 a          844 c          762 g         1423 t
ORIGIN      
        1 ttgatctctt tggcgaccgc gtctttgtcg aagctgtttt tcagacggcc tgagtgttga
       61 tccgcagttt cggtgtcgat gccggtgtag atacgcgctt cggtttcttt tgcagtcctg
      121 cctgttcggg caagttgtcc cgcttcgtcg gtgatgtgta tgttgcgggt gttgatgccg
      181 cttttcgtga tgctgctttg actgtcttgg tcgctgccat aacccactga tgaacttgcg
      241 ccgtttttat cggctacgct tgtcaggtgt ttgttttgag gtttattttt tgcgccctgt
      301 cccagtgttt tgccgcttat ggacgcactt gcgcccaatc caaaactttc gcctttgtgt
      361 tggctgtggt tttgaatgtc gctggcagta agggtggccg tctgaaagcg gtttttaccc
      421 ttgtcttctg cgctttggct gctggtgatg atgccgcctt tgaggtctgt atggtttccg
      481 accttgattt gatagccgtc ttctccggca taaataccgc tttgctcggt taccgaagca
      541 tggtcggctc ggattttgct ttggctgtaa tcgccactgg cactgaagcc ataacctacg
      601 gtcacttgtg cactggcgtt ttgttgtttg ctttgatagg tttcagtatc ttgaacactt
      661 tctatatgca ggttgcgcgt atctgcctgt atgcctttgc cgatgagctg cgcacctttg
      721 agggtggtat ccccgccgct tcgaatggta gttttaccgg ttgtgctgcc gacatgggtg
      781 tggcggtggg ttgcttttgt ctgattgctg tggtggcttg ttgaaagaaa ggctgtctga
      841 aacgtatttg ttgtttcaga cagcctcctg gctcaaacct tgaaaactac atatgtgcgt
      901 tccgcacatc ctacgtattg agtttaggtt tcacatgagc tacggcttgc tatgccgtct
      961 tttttccagg tgtggccgcg gctttggttt tcggcaaggt aggcgtttac ttggtcagat
     1021 ggcggatttt ttgttcgtcg tagatgatgg agacgctgat accggagtaa gcgtagattg
     1081 ggtcttgacc tcaaacctac acttgtttta cataaaattt cgtgtctcta tttgaaaaat
     1141 ctaaataaca acattctact ttacctattg aattgattat agttgaaaca ggaatattaa
     1201 gaagcctaat acccaaatca tcaatttcaa aatcattaat tccactctta taaagatagc
     1261 ttattatttc atcattaatt tttccaagcc aattaaaaga aatatcttct aaaaaaaact
     1321 tatttggttc aaatatctct atcgcttcaa gctgattttt atcatcataa aaacaatgga
     1381 tattcaattc gggaaaaaca tccataggaa cccgagagta tgatgactta taaatttctt
     1441 gtacatcaga actaaatatt gcacgaactt gttttcgatt cagatttgat gagataatat
     1501 ttctttctat tttttttgaa ctaaaataaa aaattgccat aatttttttg cctaaacaat
     1561 attaccattt tcgtaagatg catagaacaa accatgtctt atccattttg ttccatcggc
     1621 agacagataa cgactatatc taaattttat ttttcactct cataaaaaat tttctgcaat
     1681 attcaatata tttactttct taaccatagc gtaaattcct caggcttata tatttcagta
     1741 taagtatgac ttaaaggata tgacgccgcg tgttacgagt tgcttctttt gatttcaggg
     1801 tttatataag ttatggcttg cctgggctcg aagtgataaa gagagtattt acttttcaac
     1861 tataaaaata tgagatagtt ccatgggaaa accgtaattt aagttttaat aaagcacctt
     1921 ctaggcgata taaaaatttt ctataatttt catttggttt atatttatat ataagctgta
     1981 tttcaatagt ctcatagcta ctttctccaa aatcttcaaa aatttgacct gatccaatat
     2041 attgaatata tgaatttatt ttttcttgca acaaaaacaa atgctcgtta tcccatttta
     2101 aatgatcaga aatagttaat atgagtattc cgttttcaat agatgctgaa tcaattacgt
     2161 tctcttccaa aatagacatt atcttttcct ttcaatgata actttagtag gtttaatttt
     2221 ggtctcctgt ggatagcccg gttttccctt accgaccact gttgctcccg ttctttcaat
     2281 ttcaggaaaa gcttttttct gatttttggt aagtggcgca gttattgaag ccttatatcg
     2341 gcggcagaac gggcggtaac ttccagcaca aaaaacggag tagttctttc tgtgtttttt
     2401 ctttaatttt caatgtatta tcttaatatt tcgagggtaa catatctgct aatctagtac
     2461 agtctcaaaa tatattatag attcagttga aaaataacag attcaactgt acctttctta
     2521 tacctgattt ctttaaagtt tttcccattg tcaaaactat aaaaaagtct ctcttcctca
     2581 ctagaatatc ccatttctaa tactaaacaa tctatttcac aattactccc tccgctcata
     2641 tatgcagtat caatagaaaa tatattgtat aattccagag aattatattt atctatatca
     2701 aatagttgct ctccaaaatc caaaccattg tttccacctg cataatactt ctcctcaaag
     2761 gatgatttca aactattaaa aactgtattt agtgtaaaat acccatatgg acatatctta
     2821 ggatatatat tctctcctat tattatctga atttttccat aaataaaacc attacattca
     2881 gagttttttt cccataaaat cccaaatttt cttgttgagt ccataataat attcatataa
     2941 atccttattt taataacttg ttctttgatt gctcggggta tgctctttta attcctgcct
     3001 tttgttgact tgttaaggac aagggattct tcttatcgga tgtagaacca gcccaatgcc
     3061 aaggttcatt tttcctatcg ccccccatat aacgatgaat atgtccctta tcatcaattg
     3121 caaatcgctg tttaccaatt tgtaccgaac tttcaattaa ttgaactgaa ttctttggct
     3181 caataccagc atcaaactta aaccctaact gaccaggagt atgtttaggg ttagattgat
     3241 aagtagtttc actaaccgtt ttatctggat ttttaatttt tattgagata gcttgaggaa
     3301 caggtataga agactctgct ccctgtcctt tagacgtacc aaaatcattc ttttgatgac
     3361 tcttcgacgc caatgccgta tccggaatcc tgtccccctt cgcaacattg ccgtttgcag
     3421 ggatacggat attccccgca cccgccaata agggatcgct gccggtaacg gtcggcttga
     3481 tgtttcccag attacggatg gctgcaagaa tcggggcttt tatcctcgga ttggggttga
     3541 caaggctcgt cagtccgtcg gcaacgttca gtgcaaactc ttcgtttttc cgctctccct
     3601 gcctgatttt ggtgtctttt gtcccgctgt gcagccaggt caggttgcgg tattcgggtt
     3661 tgtcctgtcg gcggtattcc tcaaacagct tcggatcgtt gtaggcttgc ggatttcttg
     3721 cgccgtagtt gcggtagtcc caagtataac ccaaggcttt gtcttcgcct ttcatgccga
     3781 ttgtggaaat aaccgtttgg tcggtatagc cgtcttggga acctttgtcc acccaacgca
     3841 gtatctgcct gcggattctc attgccgctt cttgactgct gatttttctg ccttcacgtt
     3901 tttcgacttc acgcttgagg gcttcggcat atttgtcggc caacgccatt tctttcggat
     3961 gcagctgcct attgttccaa tcgacattcg cccctactgc cgccgtaccg acattcccgc
     4021 ccgc
//
LOCUS       DAWFHY010000122         3861 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.122, whole genome shotgun sequence.
ACCESSION   DAWFHY010000122 DAWFHY010000000
VERSION     DAWFHY010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3861)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3861)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3861
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.122"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..243
                     /locus_tag="WGQ68_001955"
     CDS             <1..243
                     /locus_tag="WGQ68_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA"
                     /protein_id="HEZ0715691.1"
                     /translation="LCYSHSSYFAEVPEQYQRDASGEYVIRDGKKVETEAWKDYKSIW
                     GEPTEKDENGKYINKYIPKLINPNKPNGEKYEDKPF"
     gene            224..532
                     /locus_tag="WGQ68_001956"
     CDS             224..532
                     /locus_tag="WGQ68_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715692.1"
                     /translation="MKINHFNIALLFVSTMMLTGCFGHCADFNCTRLDPRWVKAVDTC
                     ETKNLTIVLQVLGEDLGYKSLIEVQDSKIGQHISSQCIYHSGKYDIHKDKQYEHLFAK
                     "
     gene            880..2805
                     /locus_tag="WGQ68_001957"
     CDS             880..2805
                     /locus_tag="WGQ68_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin"
                     /protein_id="HEZ0715693.1"
                     /translation="MTVGYGFSAGGDYSQSKIRADHASVTEQSGIYAGEDGYQINVQN
                     HTDLKGGLITSTQKSEDDGKNTFSTGTLTFEDLANHAEYKGEAIGLGVSGSVAGDNPN
                     QNSRIYTVNQSGMSSTMGYGKDSDKQYATTHSGINTANITIRDNEKQQQITGISAEEA
                     KRAVKADVTTDNYAEHSGSLKNNFDKDKVQSEIDLQVKVTQEFDSNRKALRAEINKAA
                     DEKRQKAEDIRRENYIDGKNGYNTAESLALDKSADKLEKVAFYTDLALGGLYGIGDST
                     ALTYVTGSAVANPAIRAGTRPRQFWFEKCDGDSLYCSNRNYDDTADRKIYDKNGQEID
                     TKDPNYTGMVEIGDKRQISDLNEIEPNKVTGSIVTTNDGILNPQNDALKNGIKQQKWE
                     VDKEGILLIYNPPTGNSISELLYAAYDKTNDLLGGRLPLTSAQKANLELNQYAESKGY
                     KIDSNNHSRGGLTRSVSDKYSVRHNLPLPYNNVQFFGSAANVDDYTNYMNEYGRKDFN
                     ISATTHYTDFVGRTPLVALRSKHIVGGNEPTGGVEDKWFLYSHSSYFAEKPTPYLVNE
                     HGFYMDKNGDLIKDNSGNPIKMTEENKLEYSTKVPNPYLDDFEQKWIKGQTHNKVSGD
                     NPSLPVPVRTKKQQGER"
     gene            2805..3236
                     /locus_tag="WGQ68_001958"
     CDS             2805..3236
                     /locus_tag="WGQ68_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715694.1"
                     /translation="MKKYFFSVCLLLTGCGVLFDDPLTRFWNGGYFLFPDKEIASDGI
                     YKTSDTDLHYYESLSLEQRKRYLKFKPIKDKCEHKYSDRTKYDYATQMANIVRCFNEQ
                     GYQSETAFILNGHTITQKGSICTKDEPKQCDNVMRSYIVLF"
     gene            3278..3529
                     /locus_tag="WGQ68_001959"
     CDS             3278..3529
                     /locus_tag="WGQ68_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002233315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715695.1"
                     /translation="MKPIYFIIFSFLLTACVVNNDDYFAEPPTPYLINEHGFYMNKKG
                     DLIRDSHGNAIKMTEENKHNFPKESANPYYEKWIKQKRK"
BASE COUNT         1416 a          665 c          780 g         1000 t
ORIGIN      
        1 ttgtgctatt cgcatagcag ttattttgcg gaagtgcctg aacagtacca aagggatgcg
       61 agcggagagt atgttattag agatggtaaa aaagttgaga cagaagcttg gaaagattat
      121 aagtcaatat ggggcgaacc gaccgaaaaa gatgaaaatg gtaaatacat caacaaatat
      181 attcctaaat taatcaaccc aaataaacca aatggagaaa aatatgaaga taaaccattt
      241 taatatagca ttactttttg tttccaccat gatgctaaca ggatgttttg gtcattgtgc
      301 ggattttaat tgcacgagac tcgatccacg ctgggtaaaa gcagttgata catgtgaaac
      361 taaaaatctc acaatagtgt tacaagtatt aggggaagat ttaggataca aatctctcat
      421 tgaggtacaa gacagtaaaa ttggtcaaca catttcttct caatgtattt accatagcgg
      481 taagtatgat attcataaag ataagcagta tgagcatttg tttgcaaaat gatattacgc
      541 caatgtttta cagaaaaacg gctataccta tacgggtgcg gacggcaaaa cttataattc
      601 gattgtgcat aataaggatt ttgtggagaa caaagggata cctttcttgc tagaaaccaa
      661 tgacaccaca caaggtatat gtaagggatt ttgctatccg catagcaatt attttacgga
      721 agtgcctgaa cagcaccaaa aggataagaa tgggaatatt gttaaaaaga atggtgaacc
      781 tgcatcaaag gcgtacaagt caatgccaaa aacctaagta ttcaaagcgt acaagataga
      841 gaaacttatc aaagcaaaca acaaaacgcc agtgcacaag tgaccgtagg ctacggcttc
      901 agtgccggtg gcgattacag ccaaagcaaa atccgagccg accatgcttc ggtaaccgag
      961 caaagcggta tttatgccgg agaagacggt tatcaaatca atgtgcagaa ccacaccgat
     1021 ttgaaaggcg gtttgattac ttccacccaa aaatcggaag acgatggcaa aaacacattc
     1081 tcaacgggta cgctcacctt tgaagaccta gcgaatcacg ccgaatataa gggtgaagcg
     1141 attggtttgg gtgtttcagg cagcgttgcg ggcgataatc caaatcaaaa cagccgtatt
     1201 tataccgtta atcaaagcgg tatgagtagc acaatgggct atggcaaaga cagcgacaaa
     1261 caatacgcca caacccattc aggcattaac actgccaata tcaccattcg tgataacgaa
     1321 aaacaacagc aaatcacagg aatatcggcg gaagaagcga aacgggctgt taaagccgat
     1381 gtaacaaccg acaactacgc cgaacattca ggcagcctga aaaacaattt cgataaagac
     1441 aaagtgcaat cagagattga tttgcaggtt aaggttacac aggagtttga tagtaatcgc
     1501 aaagccttgc gtgcagaaat caacaaagca gcagatgaaa aaagacaaaa agccgaagat
     1561 ataagaagag aaaattatat tgacggcaaa aacggttata acaccgcaga atctttggct
     1621 ttggataagt ctgccgacaa attggaaaaa gtcgcttttt acactgattt ggcattaggt
     1681 ggattgtatg gaattggcga tagcacggct ttaacttatg taacaggtag tgcagtggct
     1741 aatccagcca ttcgagcggg tacaagacca agacagtttt ggtttgagaa atgtgacggc
     1801 gatagtttgt attgttctaa tagaaattat gacgatacgg cggatagaaa aatttatgat
     1861 aaaaacgggc aagaaattga cactaaagat ccaaattaca ctggaatggt tgagattggt
     1921 gataaacggc aaatctctga tttaaatgag attgaaccta acaaagtaac tggaagcatc
     1981 gttactacaa atgacggtat attgaaccca caaaatgacg ctctcaaaaa tgggattaag
     2041 caacaaaaat gggaagtaga taaggaaggt attttgctta tctacaatcc gccaacaggc
     2101 aattctattt ctgaactttt gtatgccgct tatgataaaa ctaacgattt gttaggcggt
     2161 cgtttgcctt taacttctgc acaaaaagcg aaccttgaat taaatcaata tgcagaaagt
     2221 aaaggttata agattgatag taacaatcat agtcgtggcg gtttaactcg aagtgtttct
     2281 gataaatatt cagtcagaca taatctgcct ttaccgtata acaatgttca attcttcggt
     2341 agtgcggcaa atgtagatga ttacacaaac tacatgaacg aatatggtag gaaagatttt
     2401 aatatttctg ccacaacaca ttacacagat tttgttggta gaactccatt agttgcattg
     2461 cgtagtaagc atattgttgg tggaaatgaa ccaacaggcg gagttgaaga taaatggttc
     2521 ttatattcgc acagcagtta ttttgcagaa aaaccaaccc cataccttgt aaatgaacac
     2581 ggtttttata tggataaaaa tggcgacctg ataaaagata attcaggaaa tcctataaaa
     2641 atgacggaag aaaataaatt ggaatattca acaaaagttc caaatccata tttagatgat
     2701 tttgaacaga aatggataaa aggacaaact cataacaagg tgtcaggtga taatccgtct
     2761 cttccagttc cagttagaac aaaaaaacaa cagggagagc gataatgaaa aaatacttct
     2821 tttcagtctg cttgttatta acaggctgtg gggttttgtt tgatgaccca ctaacaagat
     2881 tttggaatgg cggttatttt ttgtttccag acaaagaaat agcaagtgat ggaatttata
     2941 aaacttcgga tactgacttg cattattacg aaagtttgtc gcttgaacaa agaaaacgct
     3001 atctaaaatt taagcctatt aaggataaat gcgaacataa gtattcagat agaacaaaat
     3061 atgattacgc tacacaaatg gcaaatatcg ttaggtgttt taatgaacaa ggctatcaat
     3121 ctgaaactgc ttttatattg aatggacata caataactca aaaaggtagt atttgcacaa
     3181 aagatgaacc caaacaatgc gataatgtta tgcgtagtta tatcgtttta ttttaattga
     3241 aatgcaactt gaaaattgac aatacaggag caacaaaatg aaaccgattt attttattat
     3301 tttttctttt ttattaactg cttgcgtggt taataatgat gattattttg ctgaaccgcc
     3361 tacaccatac ttaattaacg aacacggttt ttatatgaat aaaaaaggtg atttaattag
     3421 agatagtcac ggtaacgcaa ttaaaatgac agaagaaaat aaacacaatt tccccaaaga
     3481 atccgctaat ccttattacg aaaaatggat aaaacaaaaa cgaaaataaa taacaaagca
     3541 aacatagtgt aggcaacttg ttgcccacgc gttaatgata actttcaggc agcctgaaag
     3601 cctaaaacca caaggaaagc tccaaaaatg aacaaccgct tacacaaagt catcttcaac
     3661 cgcaacacag caagcgtagg gtgggcaact tgttgcccac gcagttcaat cagatacaca
     3721 taaaatgatg ttttaatgtg aaatatttga tttcaggctg cctgaaaaat aaaatgatgt
     3781 ggtgtgcatc aaaccgcgtg ggcaacaagt tgcccaccct acgcttgcta gaccgaatag
     3841 caggtattta catcacaggc a
//
LOCUS       DAWFHY010000123         3794 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.123, whole genome shotgun sequence.
ACCESSION   DAWFHY010000123 DAWFHY010000000
VERSION     DAWFHY010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.123"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            317..1438
                     /gene="dnaJ"
                     /locus_tag="WGQ68_001960"
     CDS             317..1438
                     /gene="dnaJ"
                     /locus_tag="WGQ68_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEZ0715696.1"
                     /translation="MSNQDFYATLGVARTATDDEIKKAYRKLAMKYHPDRNPDNKEAE
                     EKFKEVQKAYETLSDKEKRAMYDQYGHAAFEGGGQGGFGGFGGFGGAQGFDFGDIFSQ
                     MFGGGSGRAQPDYQGEDVQVGIEITLEEAAKGVKKRINIPTYEACDVCNGSGAKPGTS
                     PETCPTCKGSGTVHIQQAIFRMQQTCPTCHGAGKHIKEPCVKCRGAGRNKAVKTVEVN
                     IPAGIDDGQRIRLSGEGGPGMHGAPAGDLYVTVRIRAHKIFQRDGLDLHCELPISFAT
                     AALGGELEVPTLDGKVKLTVPKETQTGRRMRVKGKGVKSLRSSATGDLYCHIVVETPV
                     NLTDRQKELLEEFERISTGLENQTPRKKSFLDKLRDLFD"
     gene            1634..3652
                     /locus_tag="WGQ68_001961"
     CDS             1634..3652
                     /locus_tag="WGQ68_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEZ0715697.1"
                     /translation="MNKSLSSSVEEYRELTLRGMILGALITVIFTASNVYLGLKVGLT
                     FASSIPAAVISMAVLKFFKGSNILENNMVQTQASAAGTLSTIIFVLPGLLMAGYWSGF
                     PFWQTTLLCIAGGILGVIFTIPLRYAMVVKSDLPYPEGVAAAEILKVGGHEEGDNRQG
                     GSGIKELAAGGALAGLMSFCAGGLRVIADSASYWFKSGTAIFQLPMGFSLALLGAGYL
                     VGLTGGIAILLGISIAWGIAVPYFSSHIPQPSDIEMAAFAMKLWKEKVRFIGAGTIGI
                     AAVWTLLMLLKPMVEGMKMSFKSFGGGAPATERAEQDLSPKAMIFWVLSMMFILGVSF
                     YHFIGDSHITGGMAWLLVVVCTLLASVIGFLVAAACGYMAGLVGSSSSPISGVGIVSI
                     VVISLVLLLVGESGGLMADEANRKFLLALTLFCGSAVICVASISNDNLQDLKTGYLLK
                     ATPWRQQVALIIGCIVGALVISPVLELLYEAYGFTGAMPREGMDAAQALAAPQATLMT
                     TIASGIFAHNLEWVYIFTGIAIGAVLIAADLVLKKSSGGKLALPVLAVGMGIYLPPSV
                     NMPIVAGAVLAAVLKHIIGKKAENREGRLKNADRIGTLFAAGLIVGESLIGVIMAFII
                     AFSVTNGGSDAPLALNLQNWDAAASWLGLAFFVTGMIIFARRVLKAGR"
     gene            complement(3696..>3794)
                     /locus_tag="WGQ68_001962"
     CDS             complement(3696..>3794)
                     /locus_tag="WGQ68_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0715698.1"
                     /translation="DWPLQTAPLLSPKDLAGKTWAQAEKLRLTLSR"
BASE COUNT          812 a          863 c         1128 g          991 t
ORIGIN      
        1 gcaccaaaag gcccagccaa accagtgctg cccgcggata aaaaataaaa tagggggaag
       61 tctgcagccg catcaaatgc cgtctgaaca tgcgttcggg cggcgttttt ataacaaaaa
      121 cacttcatgg cggttggttt tatgcctatc taagtttttg tgtcgtgcat acctgaagat
      181 ttcagacggc atcggtttat gctgtctgaa aagtgtattc cgtttcagtt tgtaagctat
      241 ggcagtctgt ttgtcttgtg ttttgcgcaa ttgcccttat tttgagccgt gattttattt
      301 tgaattagat gaaaaaatga gtaatcaaga tttttatgcg acgctgggtg tggcaagaac
      361 agctaccgat gatgagatta aaaaagccta ccggaaattg gcgatgaaat accatcccga
      421 ccgcaatcct gacaataaag aggcggaaga gaagtttaaa gaagtacaaa aggcgtatga
      481 aactttgtcc gacaaggaaa agcgcgctat gtacgaccag tatggtcatg cggcgtttga
      541 aggcggcgga caggggggct tcggagggtt tggcggattt ggcggtgcgc agggttttga
      601 ctttggggat attttcagcc aaatgtttgg aggcggttcg gggcgcgccc agcctgatta
      661 tcagggtgag gacgttcaag tcggtatcga aatcacgctt gaagaagccg caaaaggtgt
      721 gaagaaacgc atcaatattc cgacttatga agcgtgtgat gtctgtaacg gcagcggcgc
      781 gaaaccgggg acatccccgg aaacctgccc gacttgcaaa ggttcgggta cggtgcacat
      841 ccagcaggcg attttccgta tgcagcagac ttgtccgacc tgccacggtg cgggcaaaca
      901 cattaaagaa ccttgcgtca aatgccgtgg cgcggggcgg aataaggcgg tcaagacggt
      961 ggaagtcaat attcccgccg gtatcgatga cgggcagcgt atccgtttga gcggcgaagg
     1021 cgggccgggt atgcacggtg cgcctgccgg cgacttgtat gtaaccgtcc gcattcgggc
     1081 gcataagatt ttccaacgcg acggtctgga cttgcattgc gaactgccga tcagttttgc
     1141 cacggctgct ttgggcgggg agttggaagt gccgaccttg gacggaaagg tcaagctcac
     1201 cgtccccaaa gaaacccaaa ccggcaggag gatgcgcgtg aagggtaagg gtgtcaaatc
     1261 tttacgcagc agcgcgaccg gcgatttgta ctgccatatt gttgtcgaaa cgcctgtcaa
     1321 tttgaccgac cgtcaaaaag agcttttgga agaatttgag cggatttcta ccggcttgga
     1381 aaaccaaaca ccgcgcaaga aatcgttttt agacaagctg cgcgatttgt ttgattgatt
     1441 ttaaggttcg gaaacaagca gccgtatcgg ggaatctcct tgatacggct gtttttattt
     1501 gtttaaaaat agtttttatt ttcaatgggg tatgaggcag ggtgggataa ctgtttttaa
     1561 ctgttctttt taaaacttga catcatggcg tgatgccaac aatatgtgaa cgtctgttgt
     1621 caaaggaaga ataatgaata aatctttatc cagttcggta gaagaatacc gcgagctgac
     1681 gctccgaggc atgatactcg gtgcattgat cactgtaatt tttactgcgt ccaatgttta
     1741 cctcggtttg aaagtcgggc tgacctttgc ctcgtcgatt ccggcggcgg tgatttcgat
     1801 ggcggtttta aagtttttca aaggcagcaa tattttggaa aacaacatgg tgcagaccca
     1861 agcctcggct gcgggtacgc tttcgaccat catcttcgtc ctgcccggtt tgctgatggc
     1921 gggctactgg agcggtttcc cgttctggca gacgacgctt ttatgtattg ccggcgggat
     1981 tttgggggtg attttcacca ttcctctgcg ttacgcaatg gtggtgaaaa gcgatttgcc
     2041 ttatccggaa ggtgtggcgg ctgctgaaat tttgaaagtg ggcggtcatg aagaagggga
     2101 taaccgtcag ggcggcagcg gcatcaaaga gctggcggcc ggcggtgcgt tggcgggatt
     2161 gatgagcttt tgcgccggag gtctgcgcgt gattgccgac agcgcgagtt attggtttaa
     2221 aagcggtacg gcgattttcc agctgccgat gggcttttca ctggcattgt tgggcgcggg
     2281 ctatttggtc ggactgacgg gcggtatcgc catcctgttg ggcatttcga ttgcttgggg
     2341 cattgccgtg ccgtatttct cctcacacat tccgcagcct tccgatatag aaatggcggc
     2401 gtttgcgatg aagctgtgga aggaaaaagt acgcttcatc ggcgcgggaa ccataggtat
     2461 cgcggcggtt tggacgcttt tgatgctgct caagccaatg gtggaaggca tgaagatgtc
     2521 gttcaagagt tttggcggcg gtgcgcccgc aacagaacgc gctgaacagg atttgtcgcc
     2581 taaagccatg attttctggg tattgtctat gatgtttatt ctaggcgtgt cgttttacca
     2641 ctttatcggc gattcgcaca ttacgggcgg catggcttgg cttttggtgg tcgtttgcac
     2701 gcttttggct tccgtcatcg gctttttggt cgccgccgcc tgcggttata tggcgggttt
     2761 ggtcggctcg tcttccagcc cgatttccgg cgtgggcatc gtttccatcg tcgtgatttc
     2821 gctggttttg ctgctggtgg gcgaatccgg cggtttgatg gcggatgagg caaaccgcaa
     2881 atttttgctg gcactgactt tattttgcgg ctcggcagta atctgcgtgg cttcgatctc
     2941 caatgacaac ctgcaagacc tgaaaaccgg ctacctgctc aaagccacgc cttggcggca
     3001 gcaagtcgcc ctgattatcg gctgtatcgt cggtgcgctg gttatttcac ccgtgttgga
     3061 actgctttac gaagcctacg gctttaccgg cgcaatgccg cgcgaaggta tggacgcggc
     3121 gcaggctttg gcagcccctc aagcgacttt gatgacgacc atcgcgtcgg gcattttcgc
     3181 ccacaacctt gaatgggtct atatctttac cggcatcgcg attggcgcgg tgttgattgc
     3241 cgccgatttg gtgttgaaaa aatcatcagg cggcaaactt gccctgcccg tcctcgcggt
     3301 cggtatgggc atttatctgc cgccgtccgt caatatgccc atcgtggcag gcgcggtgtt
     3361 ggcggcggtg ttgaaacaca tcatcggcaa aaaagcggaa aaccgtgaag gccgtctgaa
     3421 aaacgccgac cgtatcggta cgcttttcgc ggccggtctg attgtcggtg aaagtctgat
     3481 tggtgtgatt atggcgttta ttattgcctt ctccgtgacc aacggcggct cggacgcgcc
     3541 gctcgcgttg aatctgcaaa actgggatgc cgccgcttct tggctgggct tggcgttctt
     3601 cgttaccgga atgattattt ttgcacggcg cgtactgaag gcgggtaggt agcctgtcgg
     3661 agaaaatgcc gtctgaaacg ttcagacggc attttttatc gggaaagtgt aaggcggagc
     3721 ttttcggctt gcgcccacgt tttgccggca aggtctttgg gcgacagcag cggcgcggtt
     3781 tgaagcggcc agtc
//
LOCUS       DAWFHY010000124         3746 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.124, whole genome shotgun sequence.
ACCESSION   DAWFHY010000124 DAWFHY010000000
VERSION     DAWFHY010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.124"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(162..275)
                     /locus_tag="WGQ68_001963"
                     /pseudo
     CDS             complement(162..275)
                     /locus_tag="WGQ68_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003705522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            413..488
                     /locus_tag="WGQ68_001964"
     tRNA            413..488
                     /locus_tag="WGQ68_001964"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:447..449,aa:Glu,seq:ttc)
     gene            complement(999..1196)
                     /locus_tag="WGQ68_001965"
     CDS             complement(999..1196)
                     /locus_tag="WGQ68_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715699.1"
                     /translation="MPSENRSAGVCGALYAVLQDKAQGRAAGCIVATVFLPDGVKNTV
                     ATPTDNEKREQCRLNRVLRRA"
     gene            1257..2063
                     /gene="thiD"
                     /locus_tag="WGQ68_001966"
     CDS             1257..2063
                     /gene="thiD"
                     /locus_tag="WGQ68_001966"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002260117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEZ0715700.1"
                     /translation="MKSSFVQTLTIAGSDSGGGAGIQADLKTFQMRGVFGTCVITAVT
                     AQNTLGVSAVHLVPTETITAQIQAIREDFDIRAYKIGMLGTAEIIECVADKLKHCDFG
                     RRVLDPVMIAKGGAPLLQDSAVAALTRLLLPDTDVLTPNLPEAEALTGVHIENRKDAE
                     RAAKILLDYGVKNVVIKGGHLNGSSSGRCTDWLFTQNETLELDSPRFPTAHTHGTGCT
                     FSACITAELAKGLDVCKAVRTAKSYITAAISSPLEIGAGHGPVNHWAYRD"
     gene            complement(2113..2982)
                     /gene="tehB"
                     /locus_tag="WGQ68_001967"
     CDS             complement(2113..2982)
                     /gene="tehB"
                     /locus_tag="WGQ68_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
                     /protein_id="HEZ0715701.1"
                     /translation="MKEWIVGQSGELFCFGQMPVWKAENLPEVLLSGYSSEEGEWVCL
                     NVLQGDVEVRTQDGAAEVWSAESGDCVFAPQQVFSVKPKTDDAEIRLSLYCAAADYFH
                     KKYGMSATHSAVAAAQDTVPAGRALDMGCGQGRNALFLGLKGFEVTAVDHNPAALANV
                     AELAEAEGLNVRTLEYDLNAAALQGEFDYIVATVVLMFLMPQRVPDVIADMQAHTAAG
                     GYNLIVSAMDTADFPCPMPFPFKFKEGELKDYYRDWELDEYKEELGAMHAKDENGNPI
                     RFKFVTMLAKKPE"
     gene            3096..3533
                     /gene="rnhA"
                     /locus_tag="WGQ68_001968"
     CDS             3096..3533
                     /gene="rnhA"
                     /locus_tag="WGQ68_001968"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003689763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEZ0715702.1"
                     /translation="MDKTVYLYTDGACKGNPGAGGWGVLMRYGSHEKELFGGEAQTTN
                     NRMELTAVIEGLKSLKRRCTVIICTDSQYVKNGMENWIHGWKRNGWKTAAKQPVKNDD
                     LWKELDVLVGQHQVSWTWVKGHAGHAENERADDLANRGAAQFS"
BASE COUNT          928 a         1082 c          894 g          842 t
ORIGIN      
        1 ccgtctgaaa atttaaaagt tctagattcc cgctttcgcg ggaatgacga agtttcagac
       61 ggcatcgccc gcctgttttg atatggcggc acccccccga caaaaaaaca atccggaacg
      121 catctgaccg ttccggctta ttttcaggcg aatccgccgc atcagaacat actgcgcacg
      181 cccatattga cctgccaagt ctggcgcatc gtgtgcatcg aagacctttg cgcctcaaaa
      241 taaagctgcc ttccgttgtc ggcattacca cgcaaaaaaa tgaattgctt gatattccaa
      301 tgtttttata tgtttttata tggtgatgcg atcagacaaa cgccccctga catttgttta
      361 gacggcatcg tattgctaaa tttctataag tatgtataat gtccgtttcc acgcgcccat
      421 cgtctagagg cctaggacac tgccctttca cggcggcaac cggggttcga atccccgtgg
      481 gcgtgccaat tcaaaaacct gcttgtttca agcaggtttt tttattatga atcgtcattc
      541 ccgcaactct tcgtcattcc cgcaaaagcg ggaatctaga gcgtagggtt gaagaaaccg
      601 ttttatccga taagtttccg tgccgacagg tctggattcc cactttcgtg ggaatgacga
      661 gtcttttata acctttaagg ctgctctgcc caatggtttg gattcccgct tatgcgggaa
      721 tgacggggcg gggcgatgcc gtctgaaact tcgtcattcc cacaaccctt cgtcattccc
      781 gtgaaaacgg gaatctagaa cttctgaatt ttcagacggc tttataaata ttgccgctgc
      841 cccaagggct agattcccgc ctgcgcggga atgatgattt ggaaatcacc cgaaacccaa
      901 aaacaactga aaccgaacac actggattcc cgctttcgta ggaatgacgg aattttaggt
      961 ttctgttttt gtggaaatga cgaataaagc gtgccggttt atgctcgccg caacacgcgg
     1021 ttcagacggc attgctctct tttttcatta tcagtgggtg tagcaactgt atttttcacc
     1081 ccgtcgggca aaaatacagt tgctacgatg caccccgccg ccctgccctg tgccttgtcc
     1141 tgcaatacgg catataatgc accacaaacc cccgcgctgc ggttttcaga cggcatcgcc
     1201 gtgctttttt acaggcatta gcccttttta tcggacacaa tattaaggag gaacaaatga
     1261 aaagctcttt tgtgcaaacg cttaccatcg ccggttcgga ttcgggcggc ggtgcgggca
     1321 ttcaggcgga tttgaaaacg tttcagatgc gcggcgtgtt cggaacgtgc gtcatcaccg
     1381 ccgttaccgc gcaaaatacc ttgggcgtgt cggcggttca tctcgtcccg accgaaacca
     1441 tcaccgcaca aatccaagca atcagggaag actttgacat ccgcgcctac aaaataggaa
     1501 tgctcggcac ggcggaaatc atcgaatgcg ttgccgacaa gctgaaacac tgcgacttcg
     1561 gcaggcgcgt actcgaccct gtgatgattg ccaaaggcgg tgcgccgctg ttgcaggatt
     1621 ccgccgttgc ggcactgacg cgcctgctgc ttcccgatac ggatgtattg acccccaacc
     1681 tgcccgaggc ggaagccctg accggcgtgc atatcgaaaa ccgtaaagat gcggaacgtg
     1741 cggcaaaaat cctgcttgat tacggtgtca aaaatgtcgt catcaaaggc ggacatttga
     1801 atggcagcag cagcggacgc tgcacggatt ggctgtttac gcaaaacgaa acgctggaat
     1861 tggacagccc gcgctttccg accgcccaca cgcacggcac gggctgcacg ttttctgcct
     1921 gcattaccgc cgagttggca aaaggcttag acgtttgcaa agccgtacgg actgccaagt
     1981 cctacatcac ggcggcaatc tcaagccctt tggaaatcgg cgcgggacac ggcccggtca
     2041 atcattgggc ctatcgggac taaccgtaaa aatgccgtct gaaacaaaat gttcagacgg
     2101 catttttgag gattattcag gctttttcgc cagcatcgtt acaaatttaa accgtatcgg
     2161 attgccgttt tcgtctttgg cgtgcatcgc gcccaattct tctttatatt cgtccagctc
     2221 ccaatcccga taataatcct tcagctctcc ctctttaaat ttaaaaggga acggcatcgg
     2281 acaagggaaa tccgccgtat ccattgccga tacgattaag ttgtacccgc ccgccgccgt
     2341 atgcgcctgc atatcggcaa tcacgtcagg cacgcgctgc ggcatcagga acatcagcac
     2401 cactgttgcc acaatataat caaactcacc ctgcaaggcg gcggcgttca aatcatattc
     2461 cagcgtgcgg acgttcaaac cctccgcctc tgccagctcc gccacgtttg ccaaggcggc
     2521 gggattgtga tcgactgcag taacttcaaa tcccttcaaa ccgagaaaca gtgcgttgcg
     2581 cccctgtccg cagcccatat ccaacgccct gcccgccggt acggtatcct gagccgccgc
     2641 aaccgcagaa tgcgtggcac tcatgccgta ttttttgtga aaatagtctg ccgccgcgca
     2701 atacagcgac aaacggattt cggcatcgtc cgttttcggt ttgacagaaa acacctgctg
     2761 cggcgcaaac acgcaatcgc cgctttctgc cgaccaaacc tctgccgccc cgtcctgcgt
     2821 gcggacttcg acatcgccct gcaacacatt caggcagacc cactcccctt cctcagacga
     2881 atagcccgac aacaaaactt ccggcaggtt ttccgccttc catacgggca tctgtccgaa
     2941 acaaaacaac tcgccgcttt gacccactat ccactccttc atattcaaaa ataaagttgc
     3001 acattatatg cctattttaa tccgccgcaa tctttcagac ggcacggcgc acaaaccgct
     3061 tataatcacg ccagacacca cacaaaggca caataatgga caaaaccgtt tacctttaca
     3121 ccgacggcgc gtgcaaaggc aatcccggcg cgggcggctg gggcgtgttg atgcgctacg
     3181 gcagccacga aaaagaactt ttcggcggcg aagcgcaaac caccaacaac cgcatggagc
     3241 tgaccgccgt catcgaagga ctgaaatcgc tcaaacgccg ctgcaccgtc atcatctgca
     3301 ccgactcgca atacgtcaaa aacggcatgg aaaactggat acacggttgg aagcgcaacg
     3361 gctggaaaac cgccgccaaa cagcccgtca aaaacgacga cttgtggaaa gaactcgacg
     3421 ttctggttgg gcagcaccaa gtcagctgga cttgggttaa aggacacgcg ggacacgcgg
     3481 aaaacgaacg cgccgacgat ttggcaaacc gtggcgcagc gcagttttcc tgactgccgc
     3541 tccggcaaaa atgccgtctg aaaccgctaa tgggctttag acggcatcgt cctccaccgt
     3601 cattcccacg caggcgggaa tccaaaccgt cgggcaacag caatattcaa agattatctg
     3661 aaagtttgaa gttctagatt cccattttca cgggaatgac gaaaagttgc aggaatgacg
     3721 gagtttcagg cggcatccga ccgccc
//
LOCUS       DAWFHY010000125         3739 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.125, whole genome shotgun sequence.
ACCESSION   DAWFHY010000125 DAWFHY010000000
VERSION     DAWFHY010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3739
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.125"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            330..3578
                     /gene="nalP"
                     /locus_tag="WGQ68_001969"
     CDS             330..3578
                     /gene="nalP"
                     /locus_tag="WGQ68_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endopeptidase autotransporter NalP"
                     /protein_id="HEZ0715703.1"
                     /translation="MRTTPTFPTKTFKPTAMALAVATTLSACLGGGGGGTSAPDFNAG
                     GTGIGSNSRATTAKSAAVSYAGIKNEMCKDRSMLCAGRDDVAVTDRDAKINAPPPNLH
                     TGDFPNPNDAYKNLINLKPAIEAGYTGRGVEVGIVDTGESVGSISFPELYGRKEHGYN
                     ENYKNYTAYMRKEAPEDGGGKDIEASFDDEAVIETEAKPTDIRHVKEIGHIDLVSHII
                     GGRSVDGRPAGGIAPDATLHIMNTNDGTKNEMMVAAIRNAWVKLGERGVRIVNNSFGT
                     TSRAGTADLFQIANSEEQYRQALLDYSGGDKTDEGIRLMQQSDYGNLSYHIRNKNMLF
                     IFSTGNDAQAQPNTYALLPFYEKDAQKGIITVAGVDRSGEKFKREMYGEPGTEPLEYG
                     SNHCGITAMWCLSAPYEASVRFTRTNPIQIAGTSFSAPIVTGTAALLLQKYPWMSNDN
                     LRTTLLTTAQDIGAVGVDSKFGWGLLDAGKAMNGPASFPFGDFTADTKGTSDIAYSFR
                     NDISGTGGLIKKGGSQLQLHGNNTYTGKTIIEGGSLVLYGNNKSDMRVETKGALIYNG
                     AASGGSLNSDGIVYLADTDQSGANETVHIKGSLQLDGKGTLYTRLGKLLKVDGTAIIG
                     GKLYMSARGKGAGYLNSTGQRVPFLSAAKIGRDYSFFTNIETDGGLLASLDSVEKTAG
                     SEGDTLSYYVRRGNAARTASAAAHSAPAGLKHAVEQGGSNLENLMVELDASESSATPE
                     TVETAAADRTDMPGIRPYGATFRAAAAVQHANAADGVRIFNSLAATVYADSTAAHADM
                     QGRRLKAVSDGLDHNATGLRVIAQTQQDGGTWEQGGVEGKMRGSTQTVGIAAKTSENT
                     TAAATLGMGHSTWSENSANAKTDSISLFAGIRHDAGDIGYLKGLFSYGRYKNSISRST
                     GADEHAEGSVNGTLMQLGALGGVNVPFAATGDLTVEGGLRYDLLKQDAFAEKGSALGW
                     SGNSITEGTLVGLAGLKLSQPLSDKAVLFATAGVERDLNGRDYTVTGGFTGATAATGK
                     TGARNMPHTRLVAGLGADVEFGNGWNGLARYSYAGSKQYGNHSGRVGVGYRF"
BASE COUNT         1017 a         1009 c          996 g          717 t
ORIGIN      
        1 taaagaatcg ttctctttga gctaaggcga ggcaacgccg tactggtttt tgttcatcca
       61 ctataactag ggaaattcaa attaagttag aattatccct atgagaaaaa gccgtctaag
      121 ccggtataaa caaaataaac tcattgagct atttgtcgaa agttcaaatt tccattttaa
      181 aacaattagt aaaatcgagt ttatcctagt tgtccaagac aacccctata ataatataat
      241 tcaaaatata aaaatgggtt acatctaaac attacggaat ttttattccc tcgcctgaat
      301 tctattgtca gattcaagga gacctcatca tgcgaacgac cccaaccttc cctacaaaaa
      361 ctttcaaacc gactgccatg gcgttagctg ttgcaacaac actttctgcc tgcttaggcg
      421 gcggcggagg cggcacttct gcgcccgact tcaatgcagg cggtaccggt atcggcagca
      481 acagcagagc aacaacagcg aaatcagcag cagtatctta cgccggtatc aagaacgaaa
      541 tgtgcaaaga cagaagcatg ctctgtgccg gtcgggatga cgttgcggtt acagacaggg
      601 atgccaaaat caatgccccc cccccgaatc tgcataccgg agactttcca aacccaaatg
      661 acgcatacaa gaatttgatc aacctcaaac ctgcaattga agcaggctat acaggacgcg
      721 gggtagaggt aggtatcgtc gacacaggcg aatccgtcgg cagcatatcc tttcccgaac
      781 tgtatggcag aaaagaacac ggctataacg aaaattacaa aaactatacg gcgtatatgc
      841 ggaaggaagc gcctgaagac ggaggcggta aagacattga agcttctttc gacgatgagg
      901 ccgttataga gactgaagca aagccgacgg atatccgcca cgtaaaagaa atcggacaca
      961 tcgatttggt ctcccatatt attggcgggc gttccgtgga cggcagacct gcaggcggta
     1021 ttgcgcccga tgcgacgcta cacataatga atacgaatga tggaaccaag aacgaaatga
     1081 tggttgcagc catccgcaat gcatgggtca agctgggcga acgtggcgtg cgcatcgtca
     1141 ataacagttt tggaacaaca tcgagggcag gcactgccga ccttttccaa atagccaatt
     1201 cggaggagca gtaccgccaa gcgttgctcg actattccgg cggtgataaa acagacgagg
     1261 gtatccgcct gatgcaacag agcgattacg gcaacctgtc ctaccacatc cgtaataaaa
     1321 acatgctttt catcttttcg acaggcaatg acgcacaagc tcagcccaac acatatgccc
     1381 tattgccatt ttatgaaaaa gacgctcaaa aaggcattat cacagtcgca ggcgtagacc
     1441 gcagtggaga aaagttcaaa cgggaaatgt atggagaacc gggtacagaa ccgcttgagt
     1501 atggctccaa ccattgcgga attactgcca tgtggtgcct gtcggcaccc tatgaagcaa
     1561 gcgtccgttt cacccgtaca aacccgattc aaattgccgg aacatccttt tccgcaccca
     1621 tcgtaaccgg cacggcggct ctgctgctgc agaaataccc gtggatgagc aacgacaacc
     1681 tgcgtaccac gttgctgacg acggctcagg acatcggtgc agtcggcgtg gacagcaagt
     1741 tcggctgggg actgctggat gcgggtaagg ccatgaacgg acccgcgtcc tttccgttcg
     1801 gcgactttac cgccgatacg aaaggtacat ccgatattgc ctactccttc cgtaacgaca
     1861 tttcaggcac gggcggcctg atcaaaaaag gcggcagcca actgcaactg cacggcaaca
     1921 acacctatac gggcaaaacc attatcgaag gcggttcgct ggtgttgtac ggcaacaaca
     1981 aatcggatat gcgcgtcgaa accaaaggtg cgctgattta taacggggcg gcatccggcg
     2041 gcagcctgaa cagcgacggc attgtctatc tggcagatac cgaccaatcc ggcgcaaacg
     2101 aaaccgtaca catcaaaggc agtctgcagc tggacggcaa aggtacgctg tacacacgtt
     2161 tgggcaaact gctgaaagtg gacggtacgg cgattatcgg cggcaagctg tacatgtcgg
     2221 cacgcggcaa gggggcaggc tatctcaaca gtaccggaca acgtgttccc ttcctgagtg
     2281 ccgccaaaat cgggcgggat tattctttct tcacaaacat cgaaaccgac ggtggtctgc
     2341 tggcttccct cgacagcgtc gaaaaaacag cgggcagtga aggcgacacg ctgtcctatt
     2401 atgtccgtcg cggcaatgcg gcacggactg cttcggcagc ggcacattcc gcgcccgccg
     2461 gtctgaaaca cgccgtagaa cagggcggca gcaatctgga aaacctgatg gtcgaactgg
     2521 atgcctccga atcatccgca acacccgaga cggttgaaac tgcggccgcc gaccgcacag
     2581 atatgccggg catccgcccc tacggcgcaa ctttccgcgc agcggcagcc gtacagcatg
     2641 cgaatgccgc cgacggtgta cgcatcttca acagtctcgc cgctaccgtc tatgccgaca
     2701 gtaccgccgc ccatgccgat atgcagggac gccggctgaa agccgtatcg gacgggttgg
     2761 accacaacgc tacgggtctg cgcgtcatcg cgcaaaccca acaggacggt ggaacgtggg
     2821 aacagggcgg tgttgaaggc aaaatgcgcg gcagtaccca aaccgtcggc attgccgcga
     2881 aaaccagcga aaatacgaca gcagccgcca cactgggcat gggacacagc acatggagcg
     2941 aaaacagtgc aaatgcaaaa accgacagca ttagtctgtt tgcaggcata cggcacgatg
     3001 cgggcgatat cggctatctc aaaggcctgt tctcctacgg acgctacaaa aacagcatca
     3061 gccgcagcac cggtgcggac gaacatgcgg aaggcagcgt caacggcacg ctgatgcagc
     3121 tgggcgcact gggcggtgtc aacgttccgt ttgctgcaac gggagatttg acggtcgaag
     3181 gcggtctgcg ctacgacctg ctcaaacagg atgcattcgc cgaaaaaggc agtgctttgg
     3241 gctggagcgg caacagcatc actgaaggca cactggtcgg actcgcgggt ctgaagctgt
     3301 cgcaaccctt gagcgataaa gccgtcctgt ttgcaacggc gggcgtggaa cgcgacctga
     3361 acggacgcga ctacacggta acgggcggct ttaccggcgc gactgcagca accggcaaga
     3421 cgggggcacg caatatgccg cacacccgcc tggttgccgg tctgggcgcg gatgtcgaat
     3481 tcggcaacgg ctggaacggc ttggcacgtt acagctacgc cggttccaaa cagtacggca
     3541 accacagcgg acgagtcggc gtaggctacc ggttctgacg gacaggaagc agacagccgc
     3601 aaagatcacg gtctttgcgg ctgtttctta tgaaaagaaa accctattcc aattgcctgc
     3661 ttctattgtt tcaagacttc ttccaaagat tcggcatcaa tcagatgtat agcggattaa
     3721 caaaaatcag gacaaggcg
//
LOCUS       DAWFHY010000126         3463 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.126, whole genome shotgun sequence.
ACCESSION   DAWFHY010000126 DAWFHY010000000
VERSION     DAWFHY010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.126"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(52..525)
                     /locus_tag="WGQ68_001970"
     CDS             complement(52..525)
                     /locus_tag="WGQ68_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEZ0715704.1"
                     /translation="MEQKGFTLIEMMIVVAILGIISVIAIPSYQSYIEKGYQSQLYTE
                     MVGINNISKQFILKNPLDDNQTIKSKLEIFVSDYKMNPKIAEKYNVSVHFVNKEKPRA
                     YSLVGVPKTGTGYTLSVWMNSVGDGYKCRDKASAEAYSETLSSDAGCEAFSNRKK"
     gene            complement(530..1123)
                     /locus_tag="WGQ68_001971"
     CDS             complement(530..1123)
                     /locus_tag="WGQ68_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HEZ0715705.1"
                     /translation="MRKQNTLTGIPTSDGQRGFALFIVLMVMIVVAFLVVTAAQSYNT
                     EQRISANESDRKLALSLAEAALREGELQVLDLEYDTDSKVTFSENCGKGLCTAVNVRT
                     NNDNEEAFDNIVVQGNPTVEAVKRSCTANSTNLCIDNKGTEYKKGTQSVSKMPRYIIE
                     YLGVKNGENVYRVTAKAWGKNANTVVVLQSYVSNNDE"
     gene            complement(1102..2112)
                     /locus_tag="WGQ68_001972"
     CDS             complement(1102..2112)
                     /locus_tag="WGQ68_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HEZ0715706.1"
                     /translation="MRRKMLNVPKGSYDGMKGFTIIEFLVAGMLSMIVLMAVGSSYFT
                     SRKLNDAANERLAEQQDLRNAATLIVRDARMAGGFGCFNMSEHAKNDIIVDPSKQTQP
                     APAPAKPGVKQENPLFSLEWANTNNNTNNNNIAKLIPVAESADIKYSGFTQHLNALVF
                     QYGIDDANASADTTVVSSCAKIAKPGKKISTLQEAKSALQITNDDKQNGNITLQRHVV
                     NAYAVGRIAGEEGLFRFQLDDKGKWGNPQLLVKKVRRMKVRYIYVSGCPEDDDAGKEE
                     TFKYTDKFDSSTNAVTPAGVEVLLSSGTDTKIAASSDNHIYAYRINATIRGGNVCANR
                     TL"
     gene            complement(2109..2720)
                     /gene="pilV"
                     /locus_tag="WGQ68_001973"
     CDS             complement(2109..2720)
                     /gene="pilV"
                     /locus_tag="WGQ68_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HEZ0715707.1"
                     /translation="MKNNDCLRLKNPQSGMALIEVLVAMLVLTIGILALLSVQLRTVA
                     SVREAETQTIVSQITQNLMEGMLMNPTIDSDSNKKNYNLYMRNHTLSAVDGDFAIDAI
                     KTKAQLAEEQLKRFSYELKNALPDAAAIHYAVCKDSLGAAPTLSAGSTFSQNCDGSAN
                     GDTLIKVLWVNDSAGDSDIARTNLETNGNNIVYTYQARVGGRE"
     gene            complement(2744..3421)
                     /locus_tag="WGQ68_001974"
     CDS             complement(2744..3421)
                     /locus_tag="WGQ68_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002261794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tfp pilus assembly protein FimT/FimU"
                     /protein_id="HEZ0715708.1"
                     /translation="MCTRKQQGFTLTELLIVMVIAAIMAMIALPNMSQWIASRRIASH
                     AERIANLLRFSRGEAVRLNLPVYICPVQVKKDGAPNNKCDSGKKGQGMLAFGDKNDNK
                     IYDNDTADVLLRSVVLNDDINNKRINYAFNHIAFGQTQPTADRVVWTFNQNGTFGYTK
                     DQHLTSKSSFFYSDGYIQIVLTDARAVSDADKKFRSAVVLIDSSGRVEVCPRGDRRTR
                     RTMCQYK"
BASE COUNT          906 a          857 c          698 g         1002 t
ORIGIN      
        1 ataaaaatgc cgtctgaaca ttgattttca gacggcatcg gcaaaacaac cttatttttt
       61 acgattagag aaggcttcac agccggcatc tgaggacaag gtctccgaat aggcttcggc
      121 agaagcctta tcacggcatt tgtatccgtc gcccacgctg ttcatccata ccgacaaagt
      181 ataacccgtc cccgtctttg gaacgccgac caagctgtat gcccttggtt tttccttatt
      241 gacaaaatgc accgaaacat tatatttttc ggcaattttc ggattcatct tatagtctga
      301 gacaaatatt tccagtttgc tcttgatggt ctgattatcg tccaggggat ttttcaaaat
      361 aaactgtttg gaaatattgt tgataccgac catctccgta taaagctggg actgatagcc
      421 tttttcaata taactttgat aagaaggtat ggcaatgacg ctgatgatgc cgagtatcgc
      481 gacgactatc atcatctcaa tcaatgtaaa ccctttttgt tccattttat tactcatcat
      541 tattgcttac ataagattga aggacgacca cggtattggc attcttaccc caagccttgg
      601 cagtaacccg ataaacattt tctccgttct tcacgcccaa atattcgata atataacgtg
      661 gcattttgct gacgctttgc gtgcctttct tatattccgt ccctttattg tcaatgcaca
      721 ggttggtaga atttgcagtg caagaacgct tcaccgcctc aacggtggga ttgccttgca
      781 ccacgatatt gtcaaaagcc tcttcattat cattatttgt ccgcacattc actgcggtac
      841 acagaccttt tccacagttt tcgctaaatg taaccttact gtccgtatca tattccaaat
      901 ccaaaacctg aagttcgcct tcccgcaaag ccgcctcggc caaagacaaa gccaatttcc
      961 tgtctgattc gttggcactg atccgctgct cggtattgta agactgcgcg gcagttacaa
     1021 ccaaaaaagc cacgacgatc atcaccatca gcacgataaa cagtgcaaac cccctctgtc
     1081 cgtcagaagt cgggattccc gtcaaagtgt tctgtttgcg catacatttc ccccgcgtat
     1141 tgtcgcattg atacggtaag cataaatatg attgtctgaa gaagcggcaa tcttggtatc
     1201 agtaccgcta ctcaataaaa cctccacccc ggcgggcgta acagcatttg tggagctgtc
     1261 gaatttatcc gtatatttga atgtttcctc tttgccggca tcgtcatctt caggacagcc
     1321 ggaaacatag atataccgca ctttcatacg tctaaccttt ttcacgagca actgaggatt
     1381 accccacttg cccttatcat ccaattggaa gcggaacaaa ccttcctcac cggcaatcct
     1441 gccgaccgca taggcattga ccacatgcct ttgaagggtg atatttccat tttgtttatc
     1501 atcattagta atctgtaatg cactctttgc ttcttgcaag gtagatattt tcttacccgg
     1561 ttttgctatt ttggcacagc tgctgacgac ggtagtatcg gcgcttgcat tagcatcatc
     1621 gataccgtat tggaaaacca atgcgttaag gtgctgggta aaacccgaat atttaatatc
     1681 tgcggattca gcaacgggaa tcaatttagc tatattatta ttattagtat tattattagt
     1741 attagcccac tctaaagaaa aaaggggatt ttcttgtttg acaccgggtt ttgcaggggc
     1801 aggggcaggt tgagtttgct tacttggatc aacaataata tcgtttttag catgctcgga
     1861 catattgaaa caaccgaagc cccctgccat tcttgcatcg cggacaatca atgttgccgc
     1921 attccgcaaa tcctgttgct cggcaagacg ctcgtttgcc gcatcattta atttccggga
     1981 tgtgaagtaa ctcgatccga ccgccatcag gacaatcata ctgagcatgc ccgcaaccaa
     2041 aaattcaata atggtaaaac ccttcatacc atcataactg ccttttggta cgtttagcat
     2101 tttacgtctc attcccgacc tccgaccctt gcctgatagg tatatacgat attgttgccg
     2161 ttcgtctcaa gattcgtacg ggcgatatcc gaatcccctg ccgaatcatt tacccacaat
     2221 actttaatca aagtatcccc atttgcacta ccatcgcaat tttgagaaaa agtactgccg
     2281 gcggacaatg tcggcgcagc acccaacgaa tccttgcaga cggcgtaatg gatggctgcc
     2341 gcatccggca aggcattttt cagctcataa ctaaatctct tcaattgttc ctctgccaac
     2401 tgcgccttag tttttatggc atcaatcgca aaatcgccat ccacagctga tagtgtatgg
     2461 tttctcatgt aaagattata gtttttcttg ttgctgtccg aatcaatggt cggattcatc
     2521 aacattcctt ccatcaggtt ttgcgtgatt tggctgacga tggtttgcgt ctccgcctcc
     2581 ctgacggaag cgactgtccg caactgtacg gacaatagtg ccaaaatacc gatggtcaga
     2641 acgagcatag caaccaagac ttctatcagc gccataccgg actggggatt tttcaggcgc
     2701 aagcaatcat tattcttcat attcattttt aaaactgaaa ctgttattta tactggcaca
     2761 tagtacgccg agtacgccga tcacccctag ggcaaacttc gaccctgccg ctgctgtcaa
     2821 tcaaaaccac cgccgaacgg aatttcttat cggcatctga aactgctctc gcatctgtca
     2881 gcacgatttg gatataaccg tcggaataaa aaaagctgga tttgcttgta agatgctggt
     2941 ctttcgtata accgaacgtc ccgttttgat tgaatgtcca aactacacgg tcagcggtcg
     3001 gctgagtctg accgaaagcg atatggttga aggcataatt aatccgctta ttattgatat
     3061 catcattcaa taccacactg cggagaagaa catccgccgt atcattgtca tatatcttat
     3121 tgtcattctt atcgccaaaa gccaacattc cctgcccctt cttgccggag tcacatttat
     3181 tgttgggcgc accgtctttt ttaacttgaa caggacagat atagacaggg agattgagcc
     3241 ggacggcttc gcccctggag aaacgcaaaa ggttggcaat ccgctccgcg tgactggcaa
     3301 tgcggcggga tgcaatccat tggctcatat tggggagggc tatcatcgcc ataatggctg
     3361 caatgaccat cacgatgagc agctctgtta gcgtgaaacc ttgttgtttt cgtgtacaca
     3421 taggcaatag aacgttaact ggtaatgtat cgtggattaa att
//
LOCUS       DAWFHY010000127         3446 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.127, whole genome shotgun sequence.
ACCESSION   DAWFHY010000127 DAWFHY010000000
VERSION     DAWFHY010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.127"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..2191
                     /locus_tag="WGQ68_001975"
     CDS             <1..2191
                     /locus_tag="WGQ68_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0715709.1"
                     /translation="YSKAHDTTTQVMKTALPSRVVAESANLQSGWDTKLQGTQFETTL
                     GGATIRAGVGEQARADAKIILEGIKTTIHNETVSSSKSALWQKQAGRGSNIETLQLPS
                     FTGPVAPVLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQTAKNVDWKQVQLAY
                     DKWDYKQEGLTGAGAAIVVIIVTALTYGYGAAAAGSVTAAGSSTAAAATTTAAATTTV
                     STAAAMQTAALASLYSQAAVAIINNKGDVGKALKDLGTSDTVKQIVTSALTAGALNQM
                     GADIAQLNSKVRTELFSSTGNQTIANLGGRLATNLSNAGISAGINTAVNGGSLKDNLG
                     NAALGALVNSFQGEAASKIKTTFSDDYVAKQFAHALAGCVSGLVQGKCKDGAIGAAVG
                     EIVAESILGGRNPATLSDAEKHKVISYSKIIAGSVAALNGGDVNTAANAAEVAVVNNA
                     LNFDSTPTNAKKHQPQKPDKTALEKIIQGIMPAHAAGAMTNPQDKDAAIWISNIRNGI
                     TGPIVITSYGVYAAGWTAPLIGTAGKAAISTCMANPSGCTVMVTQAAEAGAGIATGAV
                     TVGNAWEAPVGALSKAKAAKQAIPTQTVKELDGLLQESKNIGAVNTRINIANSTTRYT
                     PMRQTGQPVSAGFEHVLEGHFHRPIANNRSVFTISPNELKVILQSNKVVSSPVSMTPD
                     GQYMRTVDVGKVIGTTSIKEGGQPTTTIKVFTDKSGNLITTYPVKGN"
     gene            2198..2707
                     /locus_tag="WGQ68_001976"
     CDS             2198..2707
                     /locus_tag="WGQ68_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715710.1"
                     /translation="MSNFEKKYILELNDALSHLNHNSTSFDLLKVLISWLSNDIVIDK
                     FKILGYDFSKYIEMNPDDYPVEKSILNREEIIYLKNNIYRKISSGNFKFQYFVQYIRD
                     ILEYLFIEHIERVCPYCEWGEMQKLEEQNTHETVYLCTQCGCAFYNDNSQFLLKTPLT
                     IPMKRDEFK"
     gene            2776..>3446
                     /locus_tag="WGQ68_001977"
     CDS             2776..>3446
                     /locus_tag="WGQ68_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF637 domain-containing protein"
                     /protein_id="HEZ0715711.1"
                     /translation="MIYGSMPSEKLTIFQTAFVMQVNIQIPCMLYRRGSVKPPLFEAP
                     RLLPSFTDPVVPKLSAPGGYIVDIPKGNLKTEIEKLAKQPEYAYLKQLQVAKNVNWNQ
                     VQLAYDKWDYKQEGLTRAGAAIIALAVTVVTAGAGVGAALGLNGAAAAAADAAFASLA
                     SQASVSLINNKGDVGKTLKELGRSSTVKNLVVAAATAGVADKIGASALNNVSDKQWIN
                     NLTVNL"
BASE COUNT         1046 a          801 c          815 g          784 t
ORIGIN      
        1 ctacagcaaa gcacacgaca ccaccaccca agtcatgaaa accgcgctgc cctcaagggt
       61 agttgcagaa tcagccaacc tccaatcggg ctgggatacc aaactgcaag gcacacagtt
      121 tgaaaccaca ctgggtggcg caaccatacg cgcaggcgta ggtgagcagg cacgggcaga
      181 tgccaagatt atcctcgaag gcatcaaaac cacgatccac aacgaaaccg tgagcagcag
      241 caaatctgct ctatggcaaa aacaggcagg acggggcagt aacatcgaaa ccttgcaatt
      301 gccgagtttc accggtcccg ttgcgcccgt actgtccgca cccggcggtt acattgtcga
      361 tattcccaaa ggcaatctga aaaccgaaat cgaaaagctg gccaaacagc ccgaatacgc
      421 ctacctgaaa cagcttcaga cggccaagaa cgtcgattgg aaacaggtgc agctggccta
      481 cgacaaatgg gactataaac aggaaggctt gaccggagcc ggtgcagcga ttgtggtgat
      541 tattgtaacc gctctaactt atggatacgg agcggctgca gcgggtagcg taactgccgc
      601 aggaagtagt acagccgcag ctgcaacaac gacagcggca gcaactacca ctgtttctac
      661 tgcagctgcc atgcaaaccg cagctttagc ctccttgtat agccaagcag ctgtagccat
      721 catcaataat aaaggcgatg taggcaaagc attaaaagat ctcggcacca gtgatacggt
      781 caagcagatt gttacttccg cactgacggc gggtgcatta aaccagatgg gagcagatat
      841 tgcccaattg aacagcaagg taagaaccga actgttcagc agtacgggca atcaaaccat
      901 tgccaacctt ggaggcagat tggccaccaa cctcagcaat gcaggtatct cagctggtat
      961 caataccgcc gttaacggcg gcagcttgaa agacaactta ggcaatgccg cattgggagc
     1021 attggttaat agcttccaag gggaagccgc cagcaaaatc aaaacaacct tcagcgacga
     1081 ttatgttgcc aaacagttcg cccacgcttt ggcgggttgt gttagcggac tggtacaggg
     1141 aaaatgtaaa gatggggcaa ttggcgcagc agttggggaa atcgtagctg aatccatact
     1201 tggcggcaga aaccctgcta cactcagcga tgcagaaaag cataaagtta tcagttactc
     1261 gaagattatt gcgggcagcg tggcggcact caacggcggc gatgtgaata ctgcggcgaa
     1321 tgcggctgag gtagctgtgg tgaataatgc tttgaatttt gacagcaccc ctaccaatgc
     1381 gaaaaagcat caaccgcaga agcccgacaa aaccgcactg gaaaaaatta tccaaggtat
     1441 tatgcctgca catgcggcag gtgcgatgac taatccgcag gataaggatg ctgccatttg
     1501 gataagcaat atccgtaatg gcatcacagg cccgattgtg attaccagct atggagttta
     1561 tgcagcaggt tggacagctc cgctgatcgg tacagctggt aaagcagcta tcagcacctg
     1621 tatggctaat ccttctggtt gtactgtcat ggttacgcag gctgctgaag cgggcgcggg
     1681 aatcgccacg ggtgcggtaa cggtaggcaa cgcttgggaa gctccagtag gagcgttgtc
     1741 gaaagcgaag gcggccaagc aggctatacc aacccagaca gttaaagaac ttgatggctt
     1801 actacaagaa tcaaaaaata taggtgctgt aaatacacga attaatatag cgaatagtac
     1861 tactcgatat acaccaatga gacaaacggg acaaccggta tctgctggct ttgagcatgt
     1921 tcttgagggg cacttccata ggcctattgc gaataaccgt tcagttttta ccatctcccc
     1981 aaatgaattg aaggttatac ttcaaagtaa taaagtagtt tcttctcccg tatcgatgac
     2041 tcctgatggc caatatatgc ggactgtcga tgtaggaaaa gttattggta ctacttctat
     2101 taaagaaggt ggacaaccca caactacaat taaagtattt acagataagt caggaaattt
     2161 gattactaca tacccagtaa aaggaaacta actaaatatg agtaactttg aaaaaaaata
     2221 tattttagaa ttaaatgatg ctttaagcca tttaaatcat aactctacct catttgattt
     2281 attgaaagtt ttgatttcat ggttatcaaa cgatattgtc attgataaat ttaaaatttt
     2341 aggttatgac tttagtaaat atatcgaaat gaatcccgat gactatccgg ttgaaaaatc
     2401 tatattgaat agagaggaaa ttatttatct caaaaacaat atttatcgta aaatatcatc
     2461 aggaaatttt aaatttcaat actttgtaca atatattaga gatattttag aatatttatt
     2521 tattgaacat attgaaagag tctgtcctta ctgcgaatgg ggtgaaatgc aaaaattaga
     2581 agaacaaaat acgcatgaaa cggtgtatct ctgtactcaa tgtggatgtg ctttttataa
     2641 cgataattca caatttttat taaaaacccc tttaaccatt ccaatgaaac gtgatgaatt
     2701 taaataaaca agccgtagcc tgcatgaacc ctaaaatcca cgtgtagcgt gtgtgcgcca
     2761 gcacgcatgc gttccatgat ttacggctca atgccgtctg aaaagctcac aatttttcag
     2821 acggcatttg ttatgcaagt aaatattcag attccctgta tgctgtacag acgcgggagt
     2881 gttaagcccc ccttgtttga agctccgcgg ctcctgccga gcttcaccga ccccgttgtg
     2941 cccaagctct ctgctcccgg cggctacatt gtcgacatcc ccaaaggcaa tctgaaaacc
     3001 gaaatcgaaa agctggccaa acagcccgag tatgcctatc tgaaacagct ccaagtagcg
     3061 aaaaacgtca actggaacca ggtgcaactg gcttacgata aatgggacta taagcaggaa
     3121 ggcttaacca gagccggtgc agcgattatc gcgctggctg ttaccgtggt tactgcgggc
     3181 gcgggagtcg gagccgcact aggcttaaac ggcgcagccg cagcagcggc cgatgccgcc
     3241 tttgcctcac tcgcttctca ggcttccgta tcgctcatca acaataaagg cgatgtcggc
     3301 aaaaccctga aagagctggg cagaagcagc acggtgaaaa atctggtggt tgccgccgct
     3361 accgcaggcg tagccgacaa aatcggcgct tcggcactga acaatgtcag cgataagcag
     3421 tggatcaaca acctgaccgt caacct
//
LOCUS       DAWFHY010000128         3341 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.128, whole genome shotgun sequence.
ACCESSION   DAWFHY010000128 DAWFHY010000000
VERSION     DAWFHY010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.128"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            112..2877
                     /locus_tag="WGQ68_001978"
     CDS             112..2877
                     /locus_tag="WGQ68_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019742817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEZ0715712.1"
                     /translation="MRSSFRLKPICFYLMGVMLYHHSYAEDAGRAGSEAQIQVLEDVH
                     VKAKRVPKDKKVFTDARAVSTRQDIFKSSENLDNIVRSIPGAFTQQDKSSGIVSLNIR
                     GDSGFGRVNTMVDGITQTFYSTSTDAGRAGGSSQFGASVDSNFIAGLDVVKGSFSGSA
                     GINSLAGSANLRTLGVDDVVQGNNTYGLLLKGLTGTNSTKGNAMAAIGARKWLESGAS
                     VGVLYGHSRRSVAQNYRVGGGGQHIGNFGAEYLERRKQRYFVQEGGLKFNSNSGKWER
                     DFQKSYWKTKWYQKYNDPQELQKYIEGHDKSWRENLAPQYDITPIDPSSLKQQSAGNL
                     FKLEYDGVFNKYTAQFRDLNTKIGSRKIINRNYQFNYGLSLNSYANLNLTAAYNSGRQ
                     KYPKGSKFTGWGLLKDFETYNNAKILDLNNTATFRLPRETELQTTLGFNYFHNEYGKN
                     RFPEELGLFFDGPDQDNGLYSYLGRFKGDKGLLPQKSTIVQPAGSQYFNTFYFDAALK
                     KDIYRLNYSTNTVGYRFGGEYTGYYSSENEFKRAFGENSPTYREHCNPSCGLYEPVLK
                     KYGKKRANNHSVSISADFGDYFMPFASYSRTHRMPNIQEMYFSQIGDSGVHTALKPER
                     ANTWQFGFNTYKKGLLKQDDTLGLKLVGYRSRIDNYIHNVYGKWWDLNGNIPSWVSST
                     GLAYTIQHRNFKDKVHKHGFELELNYDYGRFFTNLSYAYQKSTQPTNFSDASESPNNA
                     SKEDQLKQGYGLSRVSALPRDYGRLEVGTRWLGNKLTLGGAMRYFGKSIRATAEERYI
                     DGTNGGNTSNVRQLGKRSIKQTETLARQPLIFDFYAAYEPKKNLIFRAEVKNLFDRRY
                     IDPLDAGNDAATQRYYSSFDPKDKDEDVTCNDDGLCNGKYGGTSKSVLTNFARGRTFL
                     MTMSYKF"
     gene            2907..3149
                     /locus_tag="WGQ68_001979"
     CDS             2907..3149
                     /locus_tag="WGQ68_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715713.1"
                     /translation="MPSESPSDGICFPKRIILPQAYANPFYRIGNSKKNPFHSHAGKP
                     VFDFGYFWLFRVIYESSFPRRRESRHSMLRQFIGND"
BASE COUNT          937 a          826 c          815 g          763 t
ORIGIN      
        1 caattgaaat cttcagacgg tatatcgaat ttacactttt taatgtttat gccgcctaaa
       61 aaaatgctaa tatatttcct aattgtctga ctgtttattg ttgaggaaaa tatgagatct
      121 tctttccggt tgaagccgat ttgtttttat cttatgggtg ttatgctata tcatcatagt
      181 tatgccgaag atgcagggcg cgcgggcagc gaggcgcaga tacaggtttt ggaagatgtg
      241 cacgtcaagg cgaagcgcgt accgaaagac aaaaaagtgt ttaccgatgc gcgtgccgta
      301 tcgacccgtc aggatatatt caaatccagc gaaaacctcg acaacatcgt acgcagcatc
      361 cccggtgcgt ttacacagca agataaaagc tcgggcattg tgtctttgaa tattcgcggc
      421 gacagcgggt tcgggcgggt caatacgatg gtggacggca tcacgcagac cttttattcg
      481 acttctaccg atgcgggcag ggcaggcggt tcatctcaat tcggtgcatc tgtcgacagc
      541 aattttattg ccggactgga tgtcgtcaaa ggcagcttca gcggctcggc aggcatcaac
      601 agccttgccg gttcggcgaa tctgcggact ttaggcgtgg atgacgtcgt tcagggcaat
      661 aatacctacg gcctgctgct aaaaggtctg accggcacca attcaaccaa aggtaatgcg
      721 atggcggcga taggtgcgcg caaatggctg gaaagcggag catctgtcgg tgtgctttac
      781 gggcacagca ggcgcagcgt ggcacaaaat taccgcgtgg gcggcggcgg gcagcacatc
      841 ggaaattttg gcgcggaata tctggaacgg cgcaagcagc gatattttgt acaagaaggc
      901 gggttgaaat tcaattccaa cagcggaaaa tgggagcggg atttccaaaa gtcgtactgg
      961 aaaaccaagt ggtatcaaaa atacaatgac ccccaagaac tgcaaaaata catcgaaggt
     1021 catgacaaaa gctggcggga aaacctggcg ccgcaatacg acatcacccc catcgatccg
     1081 tccagcctga agcagcagtc ggcaggtaat ctgtttaaat tggaatacga cggcgtattc
     1141 aataaataca cggcgcaatt tcgcgattta aacaccaaaa tcggcagccg caaaatcatc
     1201 aaccgcaatt atcaattcaa ttacggttta tctttaaact catatgccaa cctcaatctg
     1261 accgcagcct acaattcggg caggcagaaa tatccgaaag ggtcgaagtt tacaggctgg
     1321 ggacttttaa aggattttga aacctacaac aacgcaaaaa tcctcgacct caacaacacc
     1381 gccaccttcc ggctgccccg tgaaaccgag ttgcaaacca ctttgggctt caattatttc
     1441 cacaacgaat acggcaaaaa ccgctttcct gaagaattgg ggctgttttt cgacggtccg
     1501 gatcaggaca acgggcttta ttcctatttg gggcggttta agggcgataa agggctgctg
     1561 ccccaaaaat caaccatcgt ccaaccggcc ggcagccaat atttcaacac gttctacttc
     1621 gatgccgcgc tcaaaaaaga catttaccgc ttaaactaca gcaccaatac cgtcggctac
     1681 cgtttcggcg gcgaatatac gggctattac agctcggaaa acgaatttaa gcgggcattc
     1741 ggagaaaact cgccgacata cagggaacat tgcaacccga gctgcgggct ttatgaaccc
     1801 gtattgaaaa aatacggcaa aaagcgcgcc aacaaccatt cggtcagcat tagtgcggac
     1861 ttcggcgatt atttcatgcc gttcgccagc tattcgcgca cacaccgtat gcccaacatc
     1921 caagaaatgt atttttccca aatcggcgac tccggcgttc acaccgcctt aaaaccagag
     1981 cgcgcaaaca cttggcaatt tggcttcaat acctataaaa aaggactgtt aaaacaagat
     2041 gatacattag gattaaaact ggtcggctac cgcagccgca tcgacaacta catccacaac
     2101 gtttacggga aatggtggga tttgaacggg aatattccga gctgggtcag cagcaccggg
     2161 cttgcctaca ccatccaaca ccgcaatttc aaagacaaag tgcacaaaca cggttttgag
     2221 ttggagctga attacgatta tgggcgtttt ttcaccaacc tttcttacgc ctatcaaaaa
     2281 agcacgcaac cgaccaactt cagcgatgcg agcgaatcgc ccaacaatgc gtccaaagaa
     2341 gaccaactca aacaaggtta tgggttgagc agggtttccg ccctgccgcg agattacgga
     2401 cgtttggaag tcggtacgcg ctggttgggc aacaaactga ctttgggcgg cgcgatgcgc
     2461 tatttcggca agagcatccg cgcgacggct gaagaacgct atatcgacgg caccaacggg
     2521 ggaaatacca gcaatgtccg gcaactgggc aagcgttcca tcaaacaaac cgaaaccctt
     2581 gcccgccagc ctttgatttt tgatttttac gccgcttacg agccgaagaa aaaccttatt
     2641 ttccgcgccg aagtcaaaaa tctgttcgac aggcgttata tcgatccgct cgatgcgggc
     2701 aatgatgcgg caacgcagcg ttattacagc tcgttcgacc cgaaagacaa ggacgaagac
     2761 gtaacgtgta atgatgatgg cttgtgcaac ggcaaatatg gtggcacaag caaaagcgta
     2821 ttgaccaatt ttgcacgcgg acgcaccttt ttgatgacga tgagctacaa gttttaaagg
     2881 cagcccgcgt tttgtagaaa accgcaatgc cgtctgaaag cccttcagac ggcatttgtt
     2941 tccccaaacg catcatcctg ccgcaagcct atgccaatcc gttttatcgc atcggcaact
     3001 caaagaaaaa tccatttcat tcccacgcag ggaagccggt ttttgatttc ggttattttt
     3061 ggttgtttcg ggtaatttat gagtcgtcat tcccgcgcag gcgggaatct agacattcaa
     3121 tgctaaggca atttatcggg aatgactgaa actcaaaaaa ctggattccc actttcgtgg
     3181 gaatgacgcg gtgcaggttt ccgtatggat ggattcgtca ttcccgcgca ggcgggaatc
     3241 tagacattca acgctaaggc aatttatcgg aaatgactga aactcaaaaa actggattcc
     3301 ctctttcgtg ggaatgacgc ggtgcaggtt tccgtacgga t
//
LOCUS       DAWFHY010000129         3341 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.129, whole genome shotgun sequence.
ACCESSION   DAWFHY010000129 DAWFHY010000000
VERSION     DAWFHY010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.129"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            153..605
                     /gene="dut"
                     /locus_tag="WGQ68_001980"
     CDS             153..605
                     /gene="dut"
                     /locus_tag="WGQ68_001980"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002226436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEZ0715714.1"
                     /translation="MNIEVEMKVLDERMADFIPAYATAGSAGLDLRACLDEEVVLQPG
                     ETFLVPTGLAIYLANPAYAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGELKVSLWNR
                     SSEPFTVKPFERIAQMVIVPVVQAGFKRVEEFVGSSRGEGGFGSTGLH"
     gene            681..1868
                     /gene="dapC"
                     /locus_tag="WGQ68_001981"
     CDS             681..1868
                     /gene="dapC"
                     /locus_tag="WGQ68_001981"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003752738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyldiaminopimelate transaminase"
                     /protein_id="HEZ0715715.1"
                     /translation="MNTLLNQLKPYPFARLREAMQGISAPEGLEAVPLHIGEPKHPTP
                     KVITDALTASLHELEKYPLTAGLPELRQACANWLKRRYDGLTLNSDTEILPVLGSREA
                     LFSFVQTVLNPVSDGLKPAIVSPNPFYQIYEGATLLGGGEIHFANCPAPSFNPDWRSI
                     SEEVWKRTKLVFVCSPNNPSGSVLDLDGWKEVFDLQDKYGFIIASDECYSEIYFDGNK
                     PLGCLQAAAQLGRSRQKLLMFTSLSKRSNVPGLRSGFVAGDAELLKNFLLYRTYHGSA
                     MSIPVQRASIAAWDDEQHVIDNRRLYQEKFERVIPILQQVFDVKLPDASFYIWLKVPD
                     GDDLAFARNLWQKAAIQVLPGRFLARDTEQGNPGEGYVRIALVADVATCVKAAETIVS
                     LYR"
     gene            2031..2810
                     /gene="yaaA"
                     /locus_tag="WGQ68_001982"
     CDS             2031..2810
                     /gene="yaaA"
                     /locus_tag="WGQ68_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019272575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEZ0715716.1"
                     /translation="MFFVLSPAKNLNEKDPAPVSEFTQPDLLAESEILMQQLRELAPQ
                     QIAELMHVSDKIALLNAQRNAEWNTPFTPENAKQAVFMFNGDVYEGMDANTLDIGQIR
                     YLQNHVRLLSGLYGLLRPLDLIQPYRLEMGTAFANLRGKNLYEFWGDIITNLLNDTLA
                     QAGSNTLVNLASQEYFKSVNTKTLRAQLITPIFKDEKNGKYKIISFYAKRARGLMVRY
                     AAEHHITDPEMLKNFNYEGYAFNDAASNESEWVFMRSEQIK"
     gene            2893..2983
                     /locus_tag="WGQ68_001983"
     tRNA            2893..2983
                     /locus_tag="WGQ68_001983"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2927..2929,aa:Ser,seq:tga)
     gene            3061..3150
                     /locus_tag="WGQ68_001984"
     tRNA            3061..3150
                     /locus_tag="WGQ68_001984"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3095..3097,aa:Ser,seq:cga)
BASE COUNT          860 a          806 c          802 g          873 t
ORIGIN      
        1 gtgtgcataa tgttcgtctt cataacggga agggcgggca ttatacactg gcaacggatt
       61 tcaaaacaaa accgatttgc cgtgtttcag cgtaaacacg gcttgtgtat aatctcccat
      121 ctttgaaatc ggccgtatgc aggagcaaga cgatgaatat tgaagtagaa atgaaagtat
      181 tggacgagag gatggcggat tttatccctg cctatgcgac agcgggttct gccggtttgg
      241 acttgcgcgc ctgtttggat gaggaagtcg tcttgcagcc gggtgaaacg tttcttgtac
      301 cgacaggttt ggcaatttat ttggcgaatc ccgcatatgc cgccgttttg ctgccccgtt
      361 ccggcttggg gcataaacac ggcattgttt tgggcaattt ggtcggtttg attgactccg
      421 attatcaagg ggaattgaag gtgtcgttat ggaacagaag cagcgagccg tttactgtca
      481 aaccgttcga gcgtatcgca cagatggtta tcgttccggt cgtacaggcg ggcttcaaac
      541 gtgtcgagga atttgtcgga agcagccggg gtgagggcgg cttcggcagt acgggtttgc
      601 attgaatata aaatgccgtc tgaagggcac gtcaggttca gacggcatat cttccgatat
      661 accgacaacc ggagaaaatc atgaatacct tactcaacca actcaaaccc tatccctttg
      721 cccgactgcg tgaagcgatg cagggcattt ccgcccccga aggtctggaa gccgtcccac
      781 tgcatatcgg cgaaccgaaa catccgacac cgaaagtcat tacggatgcg ctgaccgcct
      841 cattgcacga gttggaaaaa tatccgctga cggccggttt gcctgaactg cgtcaggcgt
      901 gtgcgaactg gttaaaacgc cgttatgatg ggttgacgct gaattccgac accgaaattc
      961 tgccggtttt gggaagtagg gaggcgttgt tttcttttgt ccaaaccgtg ttgaaccctg
     1021 tttcagacgg cctcaaaccc gcaattgtca gcccgaatcc cttttatcag atttatgaag
     1081 gtgcgacact tttgggtggc ggtgaaatcc attttgctaa ttgccccgcg ccgtctttca
     1141 accccgattg gcgcagtatt tccgaagagg tttggaaacg caccaaactg gtgttcgtct
     1201 gctcgcccaa caaccccagc ggcagcgtgc tggatttgga tggatggaaa gaagtttttg
     1261 atttacagga taaatatggt ttcattattg cctcggatga atgctattcc gaaatctatt
     1321 tcgacggcaa caaaccttta ggctgcctgc aggccgctgc acagttgggt cgaagcaggc
     1381 aaaaactgct tatgttcacc agtttgtcca agcgttccaa cgttccgggc ctgcgttccg
     1441 gttttgtcgc cggcgatgcc gaactgctta aaaactttct gctttacaga acctatcacg
     1501 gcagtgcaat gagcattccc gtgcagcgcg caagcattgc cgcttgggat gacgaacagc
     1561 acgttatcga caaccgccgc ctgtatcagg aaaaatttga gcgcgttatt cccattttgc
     1621 aacaggtatt tgatgttaaa ttaccagatg cctcgtttta catttggttg aaagtccccg
     1681 atggcgacga tttggcattt gcacgcaatt tatggcaaaa agccgctatc caagtattgc
     1741 ctggacgttt tttggcacgg gataccgaac agggcaatcc cggggaaggt tatgtgcgta
     1801 ttgctttggt tgccgatgtc gcaacttgtg tcaaagctgc ggaaaccatt gtttccctat
     1861 atcggtaaag aataaaaaat accgtctgaa cttttgttca gacggcattt ttcaatattt
     1921 tacggttgaa tttgctataa cggtatttac ttcactttta atctaatcgg cttgcccgca
     1981 aacacgttta aacttaaaat ccccgtgttt gacacaatac cgagcagatt atgttttttg
     2041 tcctttcccc tgcgaaaaac cttaacgaaa aagaccctgc ccctgtcagc gagtttaccc
     2101 aacccgacct gctggcagag tccgaaattc taatgcagca gctgcgcgag cttgcgccgc
     2161 aacagattgc cgaactgatg cacgtttccg acaaaattgc cctcttaaac gcgcagcgca
     2221 atgcagaatg gaacacgccg tttacgccgg aaaacgccaa acaggcggtc tttatgttca
     2281 acggcgatgt ttacgaaggt atggatgcaa acacattgga tattggacag atacgctatc
     2341 tgcaaaacca tgtccgcctg ctgtccggtc tgtacggtct tcttcgcccg ttagacctga
     2401 tacagcccta tcgtttggaa atggggacgg catttgccaa tttgcgcggc aagaatttgt
     2461 atgagttttg gggcgacatc attaccaacc ttttaaatga cacgcttgcc caagcaggca
     2521 gcaatacgct tgtcaacctt gcctcacagg aatatttcaa gtccgtcaac acgaaaacac
     2581 ttcgggcgca gctgattacc ccgatattta aagacgaaaa aaacggtaaa tataaaatca
     2641 tcagtttcta tgccaagcgt gcgcgcggat taatggtgcg ctatgcggca gaacatcata
     2701 ttaccgaccc tgaaatgctg aaaaatttta attacgaagg ctacgcattc aatgacgcgg
     2761 cttcaaatga aagcgaatgg gtttttatgc gttcggaaca aataaagtga aaacaataaa
     2821 ttaagtattt tccgaaaaaa gtgcttggca aaatgtataa acttcattat tattcttctt
     2881 cttcaagaag acggaagcgt ggcagagcgg tttaatgcaa cggtcttgaa aaccgtcgag
     2941 ggttgatagc cctccgtgag ttcgaatctc accgcttccg ccaatccttg gggtcaaaaa
     3001 ccaaataaaa atgaagtagc ataaagcatc ggcattttta tttggtttta cttcattatc
     3061 ggaaacgtgg cagagaggct gaatgcagcg gactcgaaat ccgctgaggg tgcaaatcct
     3121 ccgtgggttc gaatcccacc gtttccgcca caaaacaaaa ccgccctgat tcggggcggt
     3181 tttgttttgt tcaagattga gacctttgca aaattccttt ccctcccgac agccgaaacc
     3241 ccaacacagg ttttcggctg ttttcgcccc aaataccgcc taattttacc caaatacccc
     3301 cttaatcctc cccggatacc cgataatcag gcatccaggc t
//
LOCUS       DAWFHY010000130         3243 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.130, whole genome shotgun sequence.
ACCESSION   DAWFHY010000130 DAWFHY010000000
VERSION     DAWFHY010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3243
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.130"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>3243
                     /locus_tag="WGQ68_001985"
     CDS             <1..>3243
                     /locus_tag="WGQ68_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HEZ0715717.1"
                     /translation="QAGKDINIIAGQISNQSEQGQTRLQAGRDINLDTVQTGKHQATH
                     FDADNHVIRGSTNEIGSNIQTQGDVTLLAGNNLNAKAAQVSSANGTLAVSAKNDINIS
                     AGINTTHVDDASKHTGRSGGGNKLIITDKAQSHNETAQSSTFEGKQVVLQAGNDANIL
                     GSNVISDNGTQIQAGNHVRIGTTQTQSQSETYHQTQKSGLMSAGIGFTIGSKTNTQEN
                     QSQSNEHTGSTVGSLKGDTTIVAGKHYEQIGSTVSSPEGNNIIHAQSIDIQAAHNKLN
                     SNTTQTYEQKGLTVAFSSPVTDLAQQAIAVAQSSKQVGQSKNGRVNAMAAANAGWQAY
                     QTGKSAQNLANGTTNAKQVSISITYGEQQNRQTTQVQASQAQASQIQAGGKTTLIATG
                     AAEQSNINIAGSDVAGKAGTILIADNDITLQSAEQSNTERSQNKSAGWNAGAAVSFGQ
                     GGWSLGVTAGGNVGKGYGNGDSVTHRHSHIGDKGSQTLIQSGGDTTIKGAQVRGKGVQ
                     VNAKNLSIQSVQDRETYQSKQQNASAQVTVGYGFSASGDYSQSKIRADHASVTEQSGI
                     YAGEDGYQIKVGNHTDLKGGIITSSQSAEDKGKNRFQTATLTTSDIQNYSQYQGKSFG
                     LGASVAVSGKTLGQGAQNKPQNKHLTSVADKNGASSSVGYGSDSDSQSSITKSGINTR
                     NIHITDEAGQIRLTGKTAAQTKADIDTNVTTDTAERHSGSLKNTFNKEAVQSELDLQR
                     EVTQEFDKTRQGVKQELYAVVDSKREQATKERLKNGGYDNDKSRALNKEANELDEKIR
                     WLDAGLGLVWGAGSSDMAWSMFATTQADRAVRSATAPKEMWFHKKIIDEKTGKVSFDT
                     RQIWSLNDLSKEELASIQDTNGKVITVSNPGIFNNREDSLSNAAKQNRNSTNGSGVIA
                     VMNPPTGKYKSDSNNKIKDSLWLSSSLVSELMYVGYDQLNNKVFQGYLPKTNSEKLNQ
                     DIYREVQKMDNGWSVDTSNHSRGGITASVSLKDWVNNKEQNGIAPIRKARFYGTATNV
                     KNDYADVLQKNGYTYTGADGKTYNSGAYSIVHDKDFVGNKWIPFLLGTNNT"
BASE COUNT         1171 a          758 c          712 g          602 t
ORIGIN      
        1 gcaggcaggc aaagacatca acatcattgc cggtcaaatc agcaatcaat cagagcaagg
       61 gcaaacccgg ctgcaagcag ggcgcgacat taacctagat acggtacaaa ccggcaaaca
      121 tcaagcaacc cattttgatg ctgataacca tgttattcgc ggttcaacta acgaaattgg
      181 tagcaatatt caaacgcaag gtgatgttac cctgttagca ggcaataacc tcaatgccaa
      241 agctgcccaa gtcagcagcg caaacggtac actcgctgtg tctgccaaaa atgacatcaa
      301 catcagcgca ggcatcaata cgacccatgt tgatgatgcg tccaaacaca caggcagaag
      361 cggtggtggc aataaattga ttattaccga caaagcccag agccataacg aaaccgctca
      421 aagcagcacc tttgaaggca agcaagttgt attgcaggca ggaaacgatg ccaacatcct
      481 tggcagcaat gttatttccg ataatggcac ccagattcaa gcaggcaatc atgttcgcat
      541 tggtacaacc caaactcaaa gccaaagcga aacctatcat caaacccaga aatcaggatt
      601 gatgagtgca ggtatcggct tcactattgg cagcaagaca aacacacaag aaaaccaatc
      661 ccaaagcaac gaacatacag gcagtaccgt aggcagcttg aaaggcgata ccaccattgt
      721 tgcaggcaaa cactacgaac aaatcggcag taccgtttcc agcccagaag gcaacaatat
      781 catccatgcc caaagcatag acattcaagc ggcacacaac aaattaaaca gtaataccac
      841 ccaaacctat gaacaaaaag gcttaacggt ggcattcagt tcgcccgtta ccgatttggc
      901 acaacaagcg attgccgtcg cacaaagcag caaacaagtc ggacaaagca aaaatggtcg
      961 tgtcaatgcc atggcagctg ccaatgcagg ctggcaagcc tatcaaacag gtaagagtgc
     1021 acaaaactta gccaatggta caaccaatgc caaacaagtc agcatctcca taacctacgg
     1081 cgaacagcaa aaccgacaaa ccacccaagt tcaagcaagc caagcccaag cgagtcaaat
     1141 tcaagcaggc ggtaaaacca cattaatcgc cacaggcgca gcagaacaat ccaatatcaa
     1201 catcgcaggc tcagatgttg ccggcaaagc aggcacaatc ctgattgccg ataacgacat
     1261 cacactccaa tcagccgagc aaagcaatac cgaacgcagc caaaacaaat cggcaggctg
     1321 gaacgcaggt gctgccgtat cattcggaca aggaggctgg tcattaggcg ttaccgcagg
     1381 cggcaatgtc ggcaaaggct acggcaatgg cgacagcgta acacaccgcc acagccatat
     1441 cggcgacaaa ggcagccaaa cccttatcca aagtggtggc gatactacca tcaaaggcgc
     1501 gcaagtacgc ggcaaaggcg tacaagtcaa tgccaaaaac ctaagtattc aaagcgtaca
     1561 agatagagaa acttatcaaa gcaaacaaca aaacgccagt gcacaagtga ccgtaggtta
     1621 tggcttcagt gccagtggcg attacagtca aagcaaaatc cgagccgacc atgcttcagt
     1681 aaccgagcaa agcggtattt atgccggaga agacggctat caaatcaagg tcggaaacca
     1741 tacagacctc aaaggcggca tcatcaccag cagccaaagc gcagaagaca agggtaaaaa
     1801 ccgctttcag acggccaccc ttactaccag cgacattcaa aattacagcc aataccaagg
     1861 aaaaagtttt ggattgggtg ccagcgttgc cgtaagcggc aaaacactgg gacagggcgc
     1921 acaaaataaa cctcaaaaca aacacctgac aagcgtagcc gataaaaacg gcgcaagttc
     1981 atcagtgggt tatggcagcg acagcgacag tcaaagcagc atcacgaaaa gcggcatcaa
     2041 cacccgcaac atacacatca ccgacgaagc gggacaaatc aggctgacag gcaaaacagc
     2101 ggcacaaacc aaagccgata ttgatacaaa cgtaaccaca gacaccgccg aacgacattc
     2161 gggcagcttg aagaacacct tcaacaaaga agcggtgcaa agtgaactgg atttacaaag
     2221 ggaagtaacc caagagtttg ataaaactag acaaggagtg aaacaagaac tttatgcagt
     2281 agtagatagc aaacgagagc aagccacaaa agagagactc aaaaatggtg gatatgacaa
     2341 tgataaatct agagcattaa ataaggaagc taatgaatta gatgaaaaaa ttcgttggct
     2401 tgatgcggga ttaggactcg tttggggagc agggagttct gatatggctt ggagtatgtt
     2461 tgccacaacc caagccgata gagcggtaag gtctgcaact gcacctaaag aaatgtggtt
     2521 ccataagaag ataatagatg aaaaaacagg taaagtatcc tttgatacca gacaaatttg
     2581 gtcattgaat gatttaagca aggaagaact ggcaagcatt caagacacaa atggcaaagt
     2641 tattactgtg tctaatcctg gtattttcaa taatcgagaa gattcattaa gcaatgcagc
     2701 aaaacaaaat cgtaatagta caaacggtag tggtgttatt gcagttatga atcctccaac
     2761 agggaaatat aaatctgatt ctaataacaa aataaaagat tctttatggc tcagttcaag
     2821 tcttgtttct gaactgatgt atgtcggtta cgaccaatta aataataaag tgttccaagg
     2881 ctatttaccc aaaaccaatt cagaaaaact gaatcaagat atttatcgag aggttcaaaa
     2941 aatggataat ggctggtctg ttgataccag taaccacagt cgtgggggaa ttacagcaag
     3001 cgtttcccta aaagattggg taaacaataa agagcaaaat ggcattgccc caatcagaaa
     3061 agcacgtttc tatggtacag ccacaaatgt gaagaatgat tacgccgatg ttttacagaa
     3121 aaacggctat acctatacgg gtgcagacgg taaaacttat aacagcggag cctactcaat
     3181 cgtgcatgat aaagattttg tggggaacaa atggatacct ttcttgctag gaaccaataa
     3241 cac
//
LOCUS       DAWFHY010000131         3175 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.131, whole genome shotgun sequence.
ACCESSION   DAWFHY010000131 DAWFHY010000000
VERSION     DAWFHY010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.131"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            52..1005
                     /locus_tag="WGQ68_001986"
     CDS             52..1005
                     /locus_tag="WGQ68_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002258530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEZ0715718.1"
                     /translation="MTHTVHLHLEETDNLALQRLCGSFDNNLDLLAKALDIHISRRFE
                     HFTFNGAFAHAGKRALLKLLETAQTRDLNDGDIRLAAVEAQTEDAGHQEKNHDHAYYF
                     RTKRGSICGRTPRQNGYIRALLNHDIVFGLGPAGTGKTYLAVAAAVDAMEKHQVERII
                     LVRPAVEAGEKLGFLPGDLTQKVDPYLRPLYDALYDLMGFDRVTKLIEKGLIEIAPLA
                     YMRGRTLNGAYIILDEAQNTTPEQMKMFLTRIGFGAKAVITGDTSQIDLPKNIKSGLK
                     DAREKLHNVEGLYFHTFTGEDVVRHPLVQKIVEAYESAEHD"
     gene            complement(1116..1439)
                     /locus_tag="WGQ68_001987"
     CDS             complement(1116..1439)
                     /locus_tag="WGQ68_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715719.1"
                     /translation="MKKCILGILTACAAMPAFADRIGDLEARLAQLEHRVAVLESGGN
                     TVKIDLFGSNSTMYVCSVTPFQKTFEASDRNEGVARQKVRQACNRETSAMFCEDEAIR
                     CRKFD"
     gene            complement(1612..2973)
                     /locus_tag="WGQ68_001988"
     CDS             complement(1612..2973)
                     /locus_tag="WGQ68_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002243435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HEZ0715720.1"
                     /translation="MLKKWLNKMLPSGRSSKKAESKTIIPAERHNIRAEMLSFAAENV
                     IRRLKGAGFQAYVVGGAVRDLLLGIEPKDFDVATDATPEQVHKLFRRSRIIGRRFQIV
                     HVMNGAEIIEVTTFRGGAKVHQNAHGRIMKDNTYGSIEEDAMRRDFTCNALYYDPEKE
                     EILDFHNGIADVAARRLVMIGDAAERYQEDPVRILRAIRLSSKLGFDLSEETAAPIAE
                     SICRLKHEPVARLFDEIMKLLFSGHARECLKRLNGFDIPDDIHPLLNALRVSDGIAGK
                     MTVLALKNTDERLRADKSVSVGFVLATLMWPELERHWKSNLQQGLKPVPALSDAINTM
                     RETVERGWGVPQRFSATMREIWMFQPQFENRKGARPHKLFAQARFRAAYDFLLLRAET
                     GNADRALAEWWTAFQTASTEQRSEMTKNEAAARHEKNEGQAKKRRRRRRKPKPKVVGT
                     DWE"
BASE COUNT          746 a          933 c          767 g          729 t
ORIGIN      
        1 taatccacta tagattaaaa tcacacaata tccaactatt cggaccttca tatgacacat
       61 accgtccacc tgcatttaga agaaaccgac aacttggcgt tgcagcgtct gtgcggttct
      121 tttgacaaca accttgattt acttgctaaa gcactcgata tccacatcag ccgccgtttt
      181 gaacatttca ctttcaacgg cgcatttgca cacgccggca aacgcgcact gctcaaactc
      241 ttggaaacgg cgcagacgcg cgacctaaac gacggcgaca tcaggcttgc cgccgtcgaa
      301 gcccaaaccg aagatgccgg tcatcaagaa aaaaaccatg accacgccta ttatttccgc
      361 accaagcgcg gcagcatctg cggcagaacg ccacggcaga acggctatat ccgcgcccta
      421 ctcaaccacg acatcgtatt cggtctcggg ccggcaggta cgggcaaaac ctatctcgca
      481 gtcgccgccg ccgtcgatgc gatggaaaaa caccaagtcg aacgcatcat tttagtgcgg
      541 cctgccgtcg aagccggcga gaaactgggc ttcctgcccg gagacctgac ccagaaagtc
      601 gatccctacc ttcgtccgct ttatgatgcc ctctatgacc tgatgggctt tgaccgtgta
      661 accaagctga ttgaaaaagg cctgattgag attgccccgc tcgcctatat gcgcggcagg
      721 acgctcaacg gcgcatacat catcctcgac gaagcgcaaa acaccacgcc cgaacaaatg
      781 aaaatgttcc tgacccgcat cggcttcggc gcgaaagccg tcattaccgg cgacaccagc
      841 caaatcgacc tgcccaaaaa catcaaatcg ggattaaaag atgcgcgtga gaaactgcac
      901 aacgtggaag ggctgtattt ccacaccttt accggcgaag acgttgtccg gcatcctttg
      961 gtgcaaaaaa tcgtcgaagc ctacgaatcg gcagaacacg actgacattc caatgccgtc
     1021 tgaaacccga tgcatggaaa atcaggcaga gaaaaacaga tacggaagcc aagctccgta
     1081 tccgtatccg tttctttatc cgtccaaccg atacatcaat cgaattttct gcatcggatt
     1141 gcctcatctt cgcaaaacat tgccgaagtt tcgcggttgc acgcctgacg cactttctgc
     1201 cgcgccacgc cttcattccg atcgcttgcc tcaaacgtct tctgaaaagg cgtaacgctg
     1261 catacataca tggtggaatt tgaaccgaaa aggtcgattt tgacggtatt gccgccgctt
     1321 tccaatacgg cgacacggtg ttccaactgc gccagacgtg cttccaaatc gccgattctg
     1381 tcggcaaatg cgggcatggc ggcacacgcg gtcaaaatgc ccaaaataca ttttttcatc
     1441 aatttttcct tgttgcttga accctgccca cccggacatc ctttttccga gaggcagggt
     1501 cagactttat ttacggagat gaggcccctt acaaatcagg ataatacata gaacggcact
     1561 ttatgcttca ccccatgtgt taaaacttca ttgctccttg tctgttgacc cttattccca
     1621 atccgttccc acaaccttcg gcttgggttt gcgcctgcga cggcggcgtt ttttcgcctg
     1681 tccttcgttt ttttcgtgtc gggcggcggc ttcgtttttg gtcatctccg accgctgttc
     1741 cgtcgatgcc gtctgaaacg ccgtccacca ctcggcaagg gcgcggtccg cattgccggt
     1801 ttcggcgcgc aagagcagga aatcataggc ggcacggaaa cgcgcctgtg caaacagttt
     1861 gtgcggcctt gcgcctttgc ggttttcaaa ctgcggctgg aacatccaaa tttcgcgcat
     1921 cgtggcggaa aagcgttgcg gtacgcccca accgcgttcg acggtttcgc gcatcgtatt
     1981 gattgcatcg gacagggcgg gcacgggttt caaaccctgt tgcagattgc ttttccaatg
     2041 gcgttccaac tcgggccaca tcagggtcgc cagtacgaaa ccgaccgaaa ccgatttgtc
     2101 ggcacgcagc cgctcatcgg tatttttcag ggcaagcacc gtcatttttc cggcgatgcc
     2161 gtctgaaacg cgcaaggcat tgagcagcgg atggatgtcg tccggtatgt caaatccgtt
     2221 caaacgtttc agacactcgc gcgcgtgccc tgaaaacagc agtttcataa tttcgtcgaa
     2281 cagccgcgct accggttcgt gcttcagacg gcatatcgat tcggcaatcg gtgcggcggt
     2341 ttcttccgac agatcaaagc ccaatttgct cgacaggcgg atggcgcgca aaatcctgac
     2401 agggtcttcc tgatagcgtt cggcggcatc accaatcata accagcctgc gggcggcaac
     2461 atcggcaatc ccgttgtgga aatccaaaat ctcttctttt tcaggatcgt aatacaaggc
     2521 attgcaggta aaatcgcgcc gcatcgcatc ttcttcgatg ctgccgtagg tgttgtcctt
     2581 catgattctg ccgtgcgcgt tctgatgaac cttcgcaccg ccgcgaaacg tcgttacttc
     2641 gataatctcc gcaccgttca tcacatggac aatctgaaaa cgcctgccga tgatgcggct
     2701 gcggcggaag agtttgtgca cctgttcggg cgtggcatcg gttgcgacat cgaaatcctt
     2761 gggttcgatg ccgagcagca ggtccctgac cgcgccgccg accacataag cctgaaaccc
     2821 cgcccctttc aggcggcgta tgacgttttc ggcggcaaag ctcaacattt cggcacggat
     2881 gttgtgtctt tcggcaggaa tgatcgtttt actttccgct tttttactgc tccgaccgga
     2941 aggcagcatc ttattcaacc attttttcag cataaatatt ctaacccgaa gagcggcatc
     3001 tccacgggca accgtgttca gacggcatca ggcggtacga ttccgcaaga tgccgtaacc
     3061 ataaacaaaa ggcgtagaaa ctacgccgtc cgaatcacgc cgccctcgcg gcggcggcaa
     3121 cgcgccatta taacagattg ccctttaaaa aaatcagacc ctgcttttgt ggcgg
//
LOCUS       DAWFHY010000132         3123 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.132, whole genome shotgun sequence.
ACCESSION   DAWFHY010000132 DAWFHY010000000
VERSION     DAWFHY010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.132"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            284..907
                     /locus_tag="WGQ68_001989"
     CDS             284..907
                     /locus_tag="WGQ68_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002229653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEZ0715721.1"
                     /translation="MKKPTDTLPVNLQRRRLLCAAGALLLSPLAHAGAQREETLADDV
                     ASVMRSSVGSVNPPRLVFDNPKEGERWLSAMSARLARFVPEEEERRRLLVNIQYESSR
                     AGLDTQIVLGLIEVESAFRQYAISGVGARGLMQVMPFWKNYIGKPAHNLFDIRTNLRY
                     GCTILRHYRNLEKGNIVRALARFNGSLGSNKYPNAVLGAWRNRWQWR"
     gene            1018..2400
                     /locus_tag="WGQ68_001990"
     CDS             1018..2400
                     /locus_tag="WGQ68_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0715722.1"
                     /translation="MARDNRIQMFPHEWRASTTLSGVYALRMLGMFLVLPVLAVYAAS
                     LPGAEGNKTLVGLAMGIYGLTQALLQLPLGIASDKFGRKKTIYVGLIVFAAGSFLAAA
                     ADTLPMLVAARAIQGAGAVSAAVTALLADLTRDGVRTRAMAMIGLSIGLTFSVSLVVA
                     PVIADAVGVRGLFLLTGILTVASIGVVAWMTPDPEVSKLHEDTQAQPSRIGEVLKNRR
                     LLTLDFGIFALHAAQMALFTALPFAMTQLGLEKIQHWKVYLPSTITGLVVMVPLIIVG
                     ETRNKLKQVFILGIACIAAAQLGLLSGMRSVGLITAYLVVYFIGFNVLEASLPSMVSK
                     IAPSDLKGTAMGVYNTMQSLGLFAGGVAGGLLFQKYGFSGVFAFCSILMLLWLVIAVL
                     SPAPKPVKNLSYPVGGVWQVNQEGLYRALSELEGVEDIGFSFDGQTVYLKVLQKGFDQ
                     AAAEKIITGV"
     gene            2404..2928
                     /locus_tag="WGQ68_001991"
     CDS             2404..2928
                     /locus_tag="WGQ68_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003710541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEZ0715723.1"
                     /translation="MSLNKVILIGRLGRDPEVRYMPNGEAVCNFSVATSETWNDRNGQ
                     RVERTEWHNITMYRKLAEIAGQYLKKGGLVYLEGRIQSRKYQGKDGIERTAYDIVANE
                     MKMLGGRNENSGGAPYEEGYGQSQEAYQRPAQQSRQPASDAPSHPQEAPAAPRRQPVP
                     AAAPVEDIDDDIPF"
BASE COUNT          629 a          837 c          942 g          715 t
ORIGIN      
        1 tgcaaaggtc tccgcctgct gccgcccgaa aagcgtgatg cgctgatggt gctgcttcac
       61 aatcaggacg acggatagcc ggtgaaaacg ggaaatgccc gggtggtgtt gtctgtgcct
      121 gtttggacaa gcgttcttga gagcggtaaa atcaagattt gcctgaaaag ggcagggcga
      181 tatgccgtgc ttggattttg tgtgcggttt tcgcgttgaa agcaattatg tctgtttgaa
      241 tatttaatgc cgtctgaaac gtccggacgc gccccagttc cctatgaaaa aaccgaccga
      301 taccctaccc gttaatctgc aacgccgccg cctgttgtgt gccgccggtg cgttgttgct
      361 cagtcctctg gcgcacgccg gcgcgcaacg tgaggaaacg cttgccgacg atgtggcttc
      421 cgtgatgagg agttctgtcg gcagcgtcaa tccgccgagg ctggtgtttg acaatccgaa
      481 agagggcgaa cgttggttgt ctgccatgtc ggcacgtttg gcaaggttcg tccccgagga
      541 ggaggagcgg cgcaggctgc tggtcaatat ccagtatgaa agcagccggg ccggtttgga
      601 tacgcagatt gtgttgggac tgattgaggt ggaaagcgcg ttccgccagt atgcaatcag
      661 cggcgtcggt gcgcgcggtc tgatgcaggt tatgccgttt tggaaaaact acatcggcaa
      721 accggcgcac aacctgttcg acatccgcac caacctgcgt tacggctgta ccatcctgcg
      781 ccattaccgg aatcttgaaa aaggcaacat cgtccgcgcg cttgcccgtt ttaacggcag
      841 cttgggcagc aataaatatc cgaacgccgt tttgggcgcg tggcgcaacc gctggcagtg
      901 gcgttgattt tgaacccgcg ccgcaaccga aatacggcga atcctgtata atccgaaaat
      961 ctgttcactg gaagttcaga cggcattgca actgttgatg ccgtctgaaa aaatatgatg
     1021 gcaagagata accgcatcca aatgtttccg cacgaatggc gcgccagtac gacgctttcc
     1081 ggcgtgtacg cgctgcgtat gctgggtatg ttcctcgtgc tgcccgtatt ggcggtgtat
     1141 gccgcctcgc tgcccggcgc ggaaggcaac aaaacgctgg tcgggctggc aatgggcatt
     1201 tacgggctga cgcaggctct gctgcaactg cctttgggca tcgcttccga caagttcggg
     1261 cgcaagaaaa ccatttatgt cgggctgatt gtgtttgcag cgggcagctt ccttgccgcc
     1321 gccgccgata cgctgcccat gctggtcgcc gcgcgcgcca tacagggtgc gggagcggtt
     1381 agtgcggcgg ttaccgcgct gctggcggat ttgacgcgcg acggcgtgcg tacccgcgcg
     1441 atggcgatga tcggtttgag tatcggtctg acgttttcgg tcagcctcgt cgttgcccct
     1501 gtgattgccg acgcggtggg cgtacgcggc ttgtttctgc tgaccggtat tttgaccgtt
     1561 gccagtatcg gcgtggtggc gtggatgact cccgacccgg aggtttccaa gctgcacgaa
     1621 gatacgcagg cgcagccttc gcgcataggc gaagttttga aaaaccgtag gctgctgacg
     1681 ctcgatttcg gcattttcgc cctgcacgcc gcgcaaatgg cattgtttac cgcgctgcct
     1741 tttgcgatga cgcagctcgg tttggaaaaa atccagcatt ggaaagtcta tctgccctcg
     1801 accattacgg gcttggtggt gatggttccg ctgattatcg tcggcgagac gcgcaacaag
     1861 ctcaaacagg tttttatctt gggcatcgcc tgcatcgcgg cggcacagct cggtttgctg
     1921 tccggtatgc gctcggtagg cttgattacc gcttatttgg ttgtttactt tatcggtttt
     1981 aatgtgttgg aagcgagcct gccgtctatg gtttccaaaa tcgcgccgtc cgacctgaag
     2041 ggtacggcga tgggcgtgta caacacgatg cagtcgctcg gactgtttgc cggcggcgtg
     2101 gcaggcggtt tgctgtttca aaaatacggc ttttccggcg tgtttgcctt ttgcagcata
     2161 ttgatgctgc tgtggctggt gattgccgtt ttatcgcctg cgcccaagcc cgtcaaaaac
     2221 ctcagttacc ctgtcggcgg cgtgtggcag gtcaatcagg aagggttata ccgcgccttg
     2281 tcggagcttg agggcgtgga agacatcggt ttcagtttcg acgggcagac cgtctatctc
     2341 aaagtgttgc agaagggttt cgatcaggct gccgctgaaa aaatcatcac aggagtttaa
     2401 aaaatgtcat tgaacaaagt catccttatc ggccgcctcg gacgcgatcc cgaagtgcgc
     2461 tatatgccca acggcgaggc ggtttgtaat ttcagcgtcg ccaccagcga aacttggaac
     2521 gaccgcaacg gccaacgtgt agagcgtact gagtggcaca acatcaccat gtaccgcaaa
     2581 ctggcggaaa ttgccgggca atacctcaaa aaaggcgggc tggtttattt ggaaggcaga
     2641 atccaaagcc gcaaatacca aggcaaagac ggcatcgaac gcaccgctta cgatattgtc
     2701 gccaacgaaa tgaaaatgtt gggcgggcgc aatgaaaaca gcggcggtgc gccttacgag
     2761 gaaggttacg gtcagagtca ggaggcttac caacgccccg cgcagcaaag ccggcagccc
     2821 gcctccgacg cgccgtccca tccccaagaa gcaccagccg cgccgcgccg ccaacccgtg
     2881 cctgccgccg ccccggtcga ggacattgac gacgatatcc cgttctgaat tttacggccg
     2941 gacatcctcg cggagggaaa tcataaagga cggagaaacc ttaaacctta cggggcaggg
     3001 tttctctttt ttgcatttgg gccggcttgt tgccgccctt tgcgttgggc ggaacgttgc
     3061 gattgcgccg ttcgggcgtt ttgaggcagt gatgcctgcc gcacgtcccc gctgttttca
     3121 gac
//
LOCUS       DAWFHY010000133         3098 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.133, whole genome shotgun sequence.
ACCESSION   DAWFHY010000133 DAWFHY010000000
VERSION     DAWFHY010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3098
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.133"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..179)
                     /locus_tag="WGQ68_001992"
     CDS             complement(<1..179)
                     /locus_tag="WGQ68_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715724.1"
                     /translation="MPSETSFDRFRRHFHTPPPPFQWAFFIKRKISRHSRPTAHFSAV
                     IPAPSPPVQTGTKTFP"
     gene            complement(255..1769)
                     /gene="katA"
                     /locus_tag="WGQ68_001993"
     CDS             complement(255..1769)
                     /gene="katA"
                     /locus_tag="WGQ68_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase KatA"
                     /protein_id="HEZ0715725.1"
                     /translation="MTTSKCPVTHLTMNNGAPVADNQNSLTAGPRGPLLAQDLWLNEK
                     LADFVREVIPERRMHAKGSGAFGTFTVTHDITKYTRAKIFSEVGKKTEMFARFTTVAG
                     ERGAADAERDIRGFALKFYTEEGNWDVVGNNTPVFFLRDPRKFPDLNKAVKRDPRTNM
                     RSATNNWDFWTLLPEALHQVTIVMSDRGIPAGYRHMHGFGSHTYSFWNEAGERFWVKF
                     HFRTQQGIKNLTNEEAAKIIADDRESHQRDLYEAIERGEFPKWTMYIQVMPEADAEKV
                     PYHPFDLTKVWPKKDYPLIEVGEFELNRNPENFFADVEQSAFAPSNLVPGVGASPDKM
                     LQARLFNYADAQRYRLGVNFRQIPVNRPRCPVHSNQRDGQGRADGNYGSLPHYEPNSF
                     GQWQQQPDFAEPPLKINGDAAHWDYRQDDDDYFSQPRALFNLMNDAQKQALFGNTAAA
                     MGDAPDFIKYRHIRNCYRCDPAYGEGVAKALGLTVEDAQAARATDPALGQPGLL"
     gene            2117..2968
                     /locus_tag="WGQ68_001994"
     CDS             2117..2968
                     /locus_tag="WGQ68_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002218621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEZ0715726.1"
                     /translation="MTLLGIKLKQTQQLNQRLQQSLRVLQMSGIELEREVENWLSDNP
                     LLERKDTDEFSDARFSHYTAPARQIGGDEGEDMLSNIAGEQDFKQYLHAQVCEHPLSD
                     QEASCVHILIDFLDEQGYLTDSIEDILDHTPLEWMLDEAMLKQALTALQKFDPAGVAA
                     ADLNESLILQIERLGECAAKPAALHIVRNALDSIDGNRSQTLARIKKHLPQTDSGTLE
                     AALDLIASLNPFPAAGFASSTPTPYSDEALANLLAFRGMEVSRRTIAKYRESLEIPAA
                     HKRKTAE"
BASE COUNT          680 a          799 c          869 g          750 t
ORIGIN      
        1 ggaaaagttt tcgtgccggt ttgaaccggt ggggatggtg cgggaatgac ggcggaaaag
       61 tgggctgtgg ggcgggaatg acgggatatt ttgcgtttaa taaaaaacgc ccactgaaac
      121 ggcgggggtg gggtgtggaa atgccgtctg aagcggtcaa acgacgtttc agacggcatt
      181 tttatgcccg gttatttccg gtagcggacg gcgcgggaca ggatttcttc aatttccatc
      241 cacataatgc ccccttacag caaaccgggc tggccgagtg cggggtcggt cgcgcgggcg
      301 gcttgtgcgt cttcgacagt cagtccgagg gcttttgcca cgccttcacc gtatgccgga
      361 tcgcaacggt agcagttgcg gatatggcgg tatttgatga agtcgggcgc gtcgcccatt
      421 gcggcggcgg tgttgccgaa caatgcctgt ttctgcgcgt cgttcatcag gttgaacagg
      481 gcgcgcggtt ggctgaagta gtcgtcatca tcttggcggt agtcccagtg tgccgcgtcg
      541 ccgttgattt tcaaaggcgg ttcggcgaag tcgggctgct gctgccattg tccgaagctg
      601 ttgggttcgt agtgcggcag gctgccgtag ttgccgtcgg ctctgccttg tccgtcgcgc
      661 tggttgctgt gaacagggca acgcggacgg ttgacaggga tttggcggaa gttcacgccc
      721 aagcggtagc gttgcgcgtc ggcgtaattg aacaaacgcg cctgcaacat tttatctggg
      781 ctggcgccga caccgggaac gaggttgctc ggtgcgaagg cggattgttc cacatcggcg
      841 aagaagtttt cgggattgcg gttcaactcg aattcgccca cttcaatcag cggatagtct
      901 tttttcggcc aaactttggt caagtcaaac ggatgataag gtactttttc cgcgtctgct
      961 tcaggcatga cttggatgta catcgtccat ttcggaaact cgccgcgttc gatggcttcg
     1021 tacaggtcgc gctgatggct ttcgcggtcg tcggcgatga ttttggcggc ttcttcgttg
     1081 gtcaggtttt taatgccttg ttgggtgcgg aaatggaatt tcacccaaaa acgctcgcct
     1141 gcttcgttcc agaagctgta ggtatgcgaa ccgaagccgt gcatatggcg gtagccggct
     1201 gggatgccgc ggtcgctcat cacgatggta acttggtgca gggcttcggg cagcagcgtc
     1261 cagaagtccc agttgtttgt ggcagagcgc atattggtgc gcggatcgcg tttgacggct
     1321 ttattcaggt cggggaactt acgcgggtcg cgcaggaaga acacgggcgt gttgttgccg
     1381 accacatccc agttgccttc ttcggtataa aatttcaatg caaaaccgcg gatgtcgcgt
     1441 tctgcatcgg ctgcgccgcg ttcgcctgcc acggtggtga aacgggcgaa catttcagtt
     1501 tttttgccga cttcgctgaa gattttggcg cgggtgtatt tggtgatgtc gtgtgttacg
     1561 gtaaacgtac cgaacgcgcc cgaacctttg gcgtgcatac ggcgttcggg aatgacttcg
     1621 cgcacgaagt cggcgagttt ttcattcagc cacaaatcct gcgccagcag ggggccgcgc
     1681 ggaccggcgg tcaggctgtt ttgattgtcg gcaacaggcg cgccgttgtt catggtcaga
     1741 tgggttacgg ggcatttgga ggtagtcatc gctcttgttc cttttctcag gttggtcaaa
     1801 tgggggtaaa cggcttacag tacgatttgg cggaaagcgt attcgtaacc ggtttcttga
     1861 ttgcaataaa tttcttgaat cgacatttta tttccctttt gtaaaaacta tggatgcgac
     1921 tatacgccaa gattttcgct attaaaacta tgaaatcgat ttaatattat tataagcaat
     1981 cggttcttga ttttcgtttg ttttttgtta tcgaacggaa tccgaacccg ctcattaaaa
     2041 ccatttataa tgcaatgacg ctttgcggca ttttttgcgc cgacaggctg aaaataacaa
     2101 ttttccccac attatcatga ccttactcgg aataaagctc aaacagaccc agcagctcaa
     2161 ccagcggctg caacaatctt tgcgcgtatt gcagatgtcg ggtatcgaac ttgaacgcga
     2221 ggtcgaaaac tggctgtcgg acaatcccct gctcgaacgc aaagacacgg atgaattttc
     2281 cgatgctagg ttttcccatt acaccgcgcc tgcccgtcaa atcggcggag acgaaggcga
     2341 agatatgctg tccaacatcg ccggtgagca ggatttcaag caatacctgc acgcgcaagt
     2401 atgcgaacac ccgctttccg accaagaagc ctcctgtgtc cacatcctta tcgatttcct
     2461 tgacgagcag ggttatctga ccgacagcat cgaagacatc ctcgaccata cgcccttaga
     2521 gtggatgttg gatgaagcaa tgctgaaaca agccctgact gcattgcaaa aattcgaccc
     2581 ggcaggcgtg gccgccgccg atttgaacga atcgctgata ctgcagatag aaagattggg
     2641 cgaatgtgct gccaaacccg ccgccctgca tatcgtccga aacgccctcg acagcattga
     2701 cggcaaccgc agccaaaccc tcgcacgaat aaaaaaacac ctgccccaaa ccgacagcgg
     2761 cacactcgaa gccgcactcg acctcattgc ttcgctcaat ccctttcccg ccgccggttt
     2821 tgcctcgtcc acacccacgc cgtattctga cgaggcactc gccaacctgc tggctttccg
     2881 cggcatggag gtttctcgcc gcaccattgc caaatacaga gaatcccttg agattccggc
     2941 agcacacaaa cgcaaaaccg cagaataatt gctgaataat cttataaaga caacaaacca
     3001 aaagccggca tttctgcgaa agcgggaatg ccgaatccgt ccgcgcggaa acctgcatcc
     3061 cgtcattccc gcgaaagagg gaatctagaa acgcaaag
//
LOCUS       DAWFHY010000134         3077 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.134, whole genome shotgun sequence.
ACCESSION   DAWFHY010000134 DAWFHY010000000
VERSION     DAWFHY010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.134"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            1017..2759
                     /locus_tag="WGQ68_001995"
     CDS             1017..2759
                     /locus_tag="WGQ68_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA family autotransporter adhesin"
                     /protein_id="HEZ0715727.1"
                     /translation="MNKIYRIIWNSALNAWVVVSELTRNHTKRASATVETAVLATLLF
                     ATVQANATDTDEDDELEPVVRSALVLQFMIDKEGNGEIESTGDIGWSIYYDDHNTLHG
                     ATVTLKAGDNLKIKQSGKDFTYSLKKELKDLTSVETEKLSFGANGNKVNITSDTKGLN
                     FAKETAGTNGDPTVHLNGIGSTLTDTLAGSSASHVDAGNQSTHYTRAASIKDVLNAGW
                     NIKGVKTGSTTGQSENVDFVRTYDTVEFLSADTKTTTVNVESKDNGKRTEVKIGAKTS
                     VIAEENGKLVTGKGDEGKGLVTAKTVIDAVNKSGWRVKTTDNNQTGQADKFETVTSGT
                     NVTFASGKGTTATVSKDDQGNITVKYDVNVGDALNVNQLQNSGWNLDSKAVAGSSGKV
                     ISGNVSPSKGKMDETVNINAGNNIEITRNGKNIDIATSMAPQFSSVSLGAGADAPTLS
                     VDDEGALNVGSKDTNKPVRITNVAPGVKEGDVTNVAQLKGVAQNLNNRIDNVDGNARA
                     GIAQAIATAGLVQAYLPGKSMMAIGGGTYRGEAGYAIGYSSISDGGNWIIKGTASGNS
                     RGHFGASASVGYQW"
     gene            2892..>3077
                     /locus_tag="WGQ68_001996"
     CDS             2892..>3077
                     /locus_tag="WGQ68_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0715728.1"
                     /translation="MSTFFQQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQRIR
                     YLRDHRGRPAYPLLSMFK"
BASE COUNT          929 a          790 c          717 g          641 t
ORIGIN      
        1 tggtttttgt taatccacta tatccgtttg cgcccgccaa gccgtgttgc acagatgcga
       61 cacggctttt tcctttctgt tgtccaaatg taaattttcg tcaaaaaaca tcaaaaatta
      121 cccccccccg aatttgcgta aaataaagtg taatcccttt gatcataagg gaatgttgac
      181 aattagatcg attctcctag gctgactttc gcaataaatg gggttgtcaa taattgtgta
      241 tggtcgtttg ccgtccggcg gccgtcattc ccgcgcaggc gggaatccag caacacaacg
      301 cagcaggaat ttatcggaaa aaacagaaac ccccacctac cgtcattccc gcgaaagcgg
      361 gaatccagca acacaacgca gcaggaattt atcggaaaaa acagaaaccc ccacctaccg
      421 tcattcccgc gaaagcggga atccagaccc gtcggcacgg aaacttaccg gataaaacag
      481 tttccttaga ttccacgtcc tagattcccg ctttcgcggg aatgacgaga ttttagatta
      541 tgggaattta tcaggaatga ttgaatccat agaaaaatca caggaatcta tcagaaaaaa
      601 cagaaacccc caccgcgtca ttccccaccg accgtcattc ccgcaaaagc gggaatccag
      661 caaccgaaaa accacaggaa tctatcagca aaaacagaaa cccccaccga ccgtcattcc
      721 cgcaaaagcg ggaatccaaa aacacaacgc aacaggaatt tatcggaaaa aacagaaacc
      781 ccaccgccgt cattcccgca aaagcgggaa tccagacccg tcggcacgga aacttaccga
      841 ataaaacagt ttccttagat tccacgtccc agattcccgc cttcgcggga atgacgagat
      901 tttaagttgg gggaatttat cagaaaaccc ccaacctcca aaaaccgggc ggatgccaca
      961 ccatccgccc ccaaaccccg atttaaccat tcaaacaaac caaaagaaaa aacaaaatga
     1021 acaaaatata ccgcatcatt tggaatagtg ccctcaatgc ctgggtcgtc gtatccgagc
     1081 tcacacgcaa ccacaccaaa cgcgcctccg caaccgtgga gaccgccgta ttggcgacgc
     1141 tgttgtttgc aacggttcag gcgaatgcta ccgataccga tgaagatgac gagttagaac
     1201 ccgtagtacg ctctgctctg gtgttgcaat tcatgatcga taaagaaggc aatggagaaa
     1261 tcgaatctac aggagatata ggttggagta tatattacga cgatcacaac actctacacg
     1321 gcgcaaccgt taccctcaaa gccggcgaca acctgaaaat caaacaaagc ggcaaagact
     1381 tcacctactc gctgaaaaaa gagctgaaag acctgaccag tgttgaaact gaaaaattat
     1441 cgtttggcgc aaacggtaat aaagtcaaca tcacaagcga caccaaaggc ttgaattttg
     1501 cgaaagaaac ggctgggacg aacggcgacc ccacggttca tctgaacggt atcggttcga
     1561 ctttgaccga tacgcttgcg ggttcttctg cttctcacgt tgatgcgggt aaccaaagta
     1621 cacattacac tcgtgcagca agtattaagg atgtgttgaa tgcgggttgg aatattaagg
     1681 gtgttaaaac tggctcaaca actggtcaat cagaaaatgt cgatttcgtc cgcacttacg
     1741 acacagtcga gttcttgagc gcagatacga aaacaacgac tgttaatgtg gaaagcaaag
     1801 acaacggcaa gagaaccgaa gttaaaatcg gtgcgaaaac ctctgttatc gctgaagaaa
     1861 acggtaagtt agttactggc aaaggcgatg agggcaaagg cttagttact gcaaaaactg
     1921 taattgacgc tgtgaacaaa agcggctgga gggtgaaaac aactgacaac aatcaaacag
     1981 gtcaagctga caagtttgaa accgttacat caggcacaaa tgtaaccttt gctagtggta
     2041 aaggtacaac tgcgactgta agtaaagatg atcaaggcaa catcactgtt aagtatgatg
     2101 taaatgtcgg cgatgcccta aacgtcaatc agctgcaaaa cagcggttgg aatttggatt
     2161 ccaaagcggt tgcaggttct tcgggcaaag tcatcagcgg caatgtttcg ccgagcaagg
     2221 gaaagatgga tgaaaccgtc aacattaatg ccggcaacaa catcgagatt acccgcaacg
     2281 gtaaaaatat cgacatcgcc acttcgatgg cgccgcagtt ttccagcgtt tcgctcggtg
     2341 cgggggcgga tgcgcccact ttgagcgtgg atgacgaggg cgcgttgaat gtcggcagca
     2401 aggataccaa caaacccgtc cgcattacca atgtcgcccc gggcgttaaa gagggggatg
     2461 ttacaaacgt cgcacaactt aaaggcgtgg cgcaaaactt gaacaaccgc atcgacaatg
     2521 tggacggcaa cgcgcgtgcg ggcatcgccc aagcgattgc aaccgcaggt ctagttcagg
     2581 cgtatctgcc cggcaagagt atgatggcga tcggcggcgg cacttatcgc ggcgaagccg
     2641 gttacgccat cggctactcc agtatttccg acggcggaaa ttggattatc aaaggcacgg
     2701 cttccggcaa ttcgcgcggc catttcggtg cttccgcatc tgtcggttat cagtggtaag
     2761 ggctttttcg cctgtctgct gttggggcag gcggaaggtt tgaagggaag ggtggcgatt
     2821 tgccgcctga gacctttgca aaattcccca aaatccccta aattcccacc aagacattta
     2881 ggggattttc catgagcacc ttctttcagc aaaccgcaca agccatgatt gccaaacaca
     2941 tcgaccgctt cccactattg aagctagatc aagtgattga ttggcaaccg atcgaacaat
     3001 acctgaaccg tcaaagaatc cgttacctcc gagaccaccg cggccgtccc gcctatccgc
     3061 tgctatccat gttcaaa
//
LOCUS       DAWFHY010000135         2998 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.135, whole genome shotgun sequence.
ACCESSION   DAWFHY010000135 DAWFHY010000000
VERSION     DAWFHY010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2998
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.135"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>2998
                     /locus_tag="WGQ68_001997"
     CDS             <1..>2998
                     /locus_tag="WGQ68_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0715729.1"
                     /translation="NVAIDANGRLVNSGTMAAANAKDTDNTAEHKVNIRSQGVENSGT
                     AVSQQGTQIHSQSIQNTGTLLSSGEILIHNSGSLKNETSGTIEAARLAIDTDTLNNQG
                     KLSQTGSQKLHIDAQGKMDNRGRMGLQDTAPTASNGSSNQTGNSYNASSHSSTTTPTT
                     ATGTGTATVSISNITAPTFADGIIRTHGALDNSGSIIANGQTGVSAQQGLNNAGQIDI
                     HQLNAKGSAFDNHNGTIISDAVHIQAGSLNNQNGNITTRQQLEIETGQLDNAHGKLLS
                     AEIADLAVSGSLNNQNGEIATNQQLIIHDGQQSTAVIDNTNGTIQSDRDVAIQAKSLS
                     NNGTLAADNKLDIALQDDFYVERNIVAGNELSLSTRGSLKNSHTLQAGKRIRIKANNL
                     DNAAQGNIQSGGATDIGTQHNLTNRGLIDGQQTKIQAGQMNNIGTGRIYGDNIAIAAT
                     RLDNQDENGTGAAIAARENLNLGIGQLNNRENSLIYSGNDMAVGGALDTNGQATGKAQ
                     RIHNAGATIEAAGKMRLGVEKLHNTNEHLKTQLVETGREHIVDYEAFGRHELLREGTQ
                     HELGWSVYNDESDHLRTPDGAAHENWHKYDYEKVTQKTQVTQTAPAKIISGNDLTIDG
                     KEVFNTDSQIIAGGNLLVQTEKDGLHNEQTFGEKKVFSENGKLHSYWREKHKGRDSTG
                     HSEQNYTLPEEITRNISLGSFAYESHRKALSHHAPSQGTELPQSNGISLPYTSNSFTP
                     LPSSSLYIINPVNKGYLVETDPRFANYRQWLGSDYMLDSLKLDPNNLHKRLGDGYYEQ
                     RLINEQIAELTGHRRLDGYQNDEEQFKALMDNGATAARSMNLSVGIALSAEQVAQLTS
                     DIVWLVQKEVKLPDGGTQTVLVPQVYVRVKNGDIDGKGALLSGSNTQINVSGSLKNSG
                     TIAGRNALIINTDTLDNIGGRIHAQKSAVTATQDINNIGGILSVEQTLLLNAGNNINS
                     QSTAASSQNTQGSSTYLDRMAGIYITG"
BASE COUNT          999 a          686 c          656 g          657 t
ORIGIN      
        1 aatgtggcga ttgatgcaaa tggccgttta gtcaatagtg gcacgatggc tgccgccaat
       61 gcgaaagata cggataatac agcggaacac aaagtcaata tccgcagtca gggcgttgaa
      121 aacagcggta cggcggtatc gcaacaaggc actcaaattc acagtcagtc gattcaaaac
      181 actggcacat tattgtcctc aggcgaaata ttgattcaca attcgggcag cctgaaaaat
      241 gaaacatcag gcaccattga agccgctcgt ttggctattg ataccgacac acttaataat
      301 caaggcaaac tctctcaaac aggttcacaa aaactccata ttgatgcaca aggcaaaatg
      361 gataaccgtg gccgcatggg tttacaagat accgcaccaa ccgcgtcaaa tggttcaagc
      421 aatcaaaccg gcaatagtta caatgcatct tcccattcat ccactaccac accaacaacg
      481 gcaacaggta cgggtactgc aaccgtttct atatcaaaca taactgcgcc tacctttgct
      541 gatgggataa ttcgcactca tggtgcactg gataattcag gcagtattat tgccaatggt
      601 caaacaggtg ttagtgcgca acaaggttta aataatgcag gacaaataga cattcatcag
      661 ttaaatgcaa aaggttcggc gtttgacaat cacaatggaa caattatcag tgatgcggtc
      721 cacattcaag ccggcagcct gaataatcaa aacggcaaca tcacaacacg ccaacagtta
      781 gagattgaaa ccggtcaact ggataacgct catggcaagt tattatcagc agaaatagcg
      841 gatttagccg tttcaggcag cctgaacaat caaaatggcg aaatagcgac caatcaacaa
      901 ctgattattc acgatggtca gcaatctacc gctgtcattg ataatacgaa tggcacgata
      961 caatcagacc gtgatgttgc tattcaggca aaatcgttat ccaacaacgg cacacttgcc
     1021 gctgataata aactggatat tgcgttacaa gatgattttt atgtagaacg caatatcgtg
     1081 gcgggcaatg aattgtcgct cagtacacga ggcagcctga aaaattcaca tactttgcaa
     1141 gcaggaaaac gcattcggat taaagcaaat aaccttgata atgcagcaca aggcaacatt
     1201 caatccggcg gtgcaacaga cattggcacg cagcacaatt taaccaatag aggcttgatt
     1261 gacggacaac aaaccaaaat ccaagccggg caaatgaata atatcggtac aggtcggatt
     1321 tatggcgaca atatcgctat tgcggctacc cgcttagaca atcaagatga aaacggtaca
     1381 ggtgccgcca ttgcggcacg tgaaaacctg aatttaggca tcggacaatt aaacaaccgt
     1441 gaaaacagtc tgatttacag cggtaacgat atggcggttg gcggcgcatt agataccaat
     1501 ggccaagcca caggcaaagc ccaaaggata cacaatgccg gcgcaaccat tgaagctgca
     1561 ggcaaaatgc gtttaggtgt agaaaagctg cacaatacca atgagcattt gaaaacgcag
     1621 ttggtagaaa cagggcgcga gcatattgtt gattacgaag catttggacg acacgaatta
     1681 ttgcgagaag gcacgcaaca tgaattaggc tggtctgtct ataacgatga atcagaccac
     1741 ttacgcaccc ctgatggagc ggcgcatgaa aattggcata aatacgatta tgaaaaagtc
     1801 acccaaaaaa cccaagttac ccaaactgcg ccagccaaaa tcatttcagg taatgattta
     1861 accattgatg gtaaagaagt atttaatacc gatagccaaa tcatcgccgg cggcaatttg
     1921 cttgtgcaaa cagaaaaaga cggtttgcat aacgagcaaa cctttggcga aaagaaagta
     1981 ttcagtgaaa atggcaaatt acacagctat tggcgtgaga aacataaagg acgagactca
     2041 acgggacata gcgaacaaaa ttacactttg ccagaggaaa tcacacgcaa catttcactg
     2101 ggttcatttg cctatgaatc gcatcgcaaa gcattaagcc atcatgcgcc cagccaaggc
     2161 actgagttgc cgcaaagcaa cggtatttcg ctaccctata cgtccaattc ttttacccca
     2221 ttacccagca gcagcttata tattatcaat cctgtcaata aaggctatct tgttgaaacc
     2281 gatccacgct ttgccaacta ccgtcaatgg ttgggtagtg actatatgct ggacagcctc
     2341 aaactagacc caaacaattt acataaacgt ttgggtgatg gttattacga gcaacgttta
     2401 atcaatgaac agatcgcaga gctgacaggg catcgtcgtt tagacggtta tcaaaacgac
     2461 gaagaacaat ttaaagcctt aatggataat ggcgcgactg cggcacgctc catgaatctc
     2521 agcgttggca ttgcattaag tgccgagcaa gtagcgcaac tgaccagcga tattgtttgg
     2581 ttggtacaaa aagaagttaa acttcctgat ggcggcacac aaaccgtatt ggtgccacag
     2641 gtttatgtac gcgttaagaa tggcgacata gacggtaaag gtgcattgtt gtcaggcagc
     2701 aatacacaaa tcaatgtttc aggcagcctg aaaaactcag gcacgattgc agggcgcaat
     2761 gcgcttatta tcaataccga tacgctagac aatatcggtg ggcgtattca tgcgcaaaaa
     2821 tcagcggtta cggccacaca agacatcaat aatattggcg gcattctttc tgtcgaacag
     2881 acattattgc tcaacgcagg caacaacatc aacagccaaa gcaccgccgc cagcagtcaa
     2941 aatacacaag gcagcagcac ctacctagac cgaatggcag gtatttatat cacaggca
//
LOCUS       DAWFHY010000136         2882 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.136, whole genome shotgun sequence.
ACCESSION   DAWFHY010000136 DAWFHY010000000
VERSION     DAWFHY010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2882
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.136"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            212..535
                     /locus_tag="WGQ68_001998"
     CDS             212..535
                     /locus_tag="WGQ68_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003775941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEZ0715730.1"
                     /translation="MQNETINLKQHLAAIKEYWQPEIINRHGFQFRLVKLLGDYGWHT
                     HGYSDKVLFAVEGDMAVDFADGGSMTIREGEMAVVPKSVSHRPRSENGCSLVLIELSD
                     PSEAV"
     gene            649..2826
                     /locus_tag="WGQ68_001999"
     CDS             649..2826
                     /locus_tag="WGQ68_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent siderophore receptor"
                     /protein_id="HEZ0715731.1"
                     /translation="MTRFKYSLLFAALLPVYAQADVSVSDDPKPQKSTELPTITVTAD
                     RTASSNDGYTVSGTHTPLGLPMTLREIPQSVSVITSQQMRDQNIKTLDRALLQATGTS
                     RQIYGSDRAGYNYLFARGSRIANYQINGIPVADALADTGNANTAAYERVEVVRGVAGL
                     LDGTGEPSATVNLVRKRPTRKPLFEVRAEAGNRKHFGLGADVSGSLNTEGTLRGRLVS
                     TFGRGDSWRQRERSRDAELYGILEYDIAPQTRVHAGMDYQQAKETADAPLSYAVYDSQ
                     GYATAFGPKDNPATNWANSRHRALNLFAGIEHRFNQDWKLKAEYDYTRSRFRQPYGVA
                     GVLSIDHNTAATDLIPGYWHADPRTHSASVSLIGKYRLFGREHDLIAGINGYKYASNK
                     YGERSIIPNAIPNAYEFSRTGAYPQPASFAQTIPQYGTRRQIGGYLATRFRAADNLSL
                     ILGGRYSRYRTGSYDSRTQGMTYVSANRFTPYTGIVFDLTGNLSLYGSYSSLFVPQSQ
                     KDEHGSYLKPVTGNNLEAGIKGEWLEGRLNASAAVYRARKNNLATAAGRDPSGNTYYR
                     AANQAKTHGWEIEVGGRITPEWQIQAGYSQSKTRDQDGSRLNPDSVPERSFKLFTAYH
                     FAPEAPSGWTIGAGVRWQSETHTDPATLRIPNPAAKARAADNSRQKAYAVADIMARYR
                     FNPRAELSLNVDNLFNKHYRTQPDRHSYGALRTVNAAFTYRFK"
BASE COUNT          699 a         1018 c          729 g          436 t
ORIGIN      
        1 tcagaccgat ggcagcccgc acccgagttt atgcggcaaa cagcgaggct acggcaaccc
       61 gtcccctctc cctgtgggag agggttaggg agagggcggt aagccgcagg cttgcatcaa
      121 agtcgataac gctttcgtta caactccgcc cactgaacga agccaaaacg acaaaccgca
      181 tcgtaaacca cccaacccat aggagaaccc catgcaaaac gaaaccatca acctgaaaca
      241 gcaccttgcc gccatcaaag aatactggca gcccgaaatc atcaaccgcc acgggttcca
      301 attccgtttg gtcaaacttt tgggcgatta cggctggcat acgcacggat acagcgacaa
      361 agtgctgttt gccgtggagg gcgacatggc ggtggacttc gccgacggcg gcagcatgac
      421 gatacgcgag ggcgagatgg cggtcgtgcc gaagtcggtg tcgcaccgcc cgcgttcgga
      481 aaacggctgc tcgttggtgc tgattgagtt gtccgacccg tccgaggccg tctgaaaacg
      541 aagtttccga aggaagctga gtttctgcga agctaaaagc agcctgcacc ttcaatcaat
      601 atgccgaaaa tacaacccca ccgcacacca acacacaaag gaaatcccat gacacgcttc
      661 aaatattccc tgctgtttgc cgccctgttg cccgtgtacg cgcaggccga tgtttctgtt
      721 tcagacgacc ccaaaccgca gaaaagcact gaattgccga ccatcaccgt taccgccgac
      781 cgcaccgcga gttccaacga cggctacact gtttccggca cgcacacccc gctcgggctg
      841 cccatgaccc tgcgcgaaat cccgcagagc gtcagcgtca tcacatcgca acaaatgcgc
      901 gaccaaaaca tcaaaacgct cgaccgcgcc ctgttgcagg cgaccggcac cagccgccag
      961 atttacggct ccgaccgcgc gggctacaac tacctgttcg cgcgcggcag ccgcatcgcc
     1021 aactaccaaa tcaacggcat ccccgttgcc gacgcgctag ccgatacggg caatgccaac
     1081 accgccgcct atgagcgcgt agaagtcgtg cgcggcgtgg cggggctgct ggacggcacg
     1141 ggcgagcctt ccgccaccgt caatctggtg cgcaaacgcc cgacccgcaa gccattgttt
     1201 gaagtccgcg ccgaagcggg caaccgcaaa catttcgggc tgggcgcgga cgtatcgggc
     1261 agcctgaaca ccgaaggcac gctgcgcggc cgcctggttt ccaccttcgg acgcggcgac
     1321 tcgtggcggc agcgcgaacg tagccgcgat gccgaactct acggcatttt ggaatacgac
     1381 atcgcaccgc aaacccgcgt ccacgcaggc atggactacc agcaggcgaa agaaaccgcc
     1441 gacgcgccgc tcagctacgc cgtgtacgac agccaaggtt atgccaccgc cttcggcccg
     1501 aaagacaacc ccgccacaaa ttgggcgaac agccgccacc gtgcgctcaa cctgttcgcc
     1561 ggcatcgaac accgcttcaa ccaagactgg aaactcaaag ccgaatacga ctacacccgc
     1621 agccgcttcc gccagcccta cggcgtagca ggcgtgcttt ccatcgacca caacaccgcc
     1681 gccaccgacc tgattcccgg ttattggcac gccgacccgc gcacccacag cgccagcgtg
     1741 tcattgatcg gcaaataccg cctgttcggc cgcgaacacg atttaatcgc gggtatcaac
     1801 ggttacaaat acgccagcaa caaatacggc gaacgcagca tcatccccaa cgccattccc
     1861 aacgcctacg aattttcccg cacgggtgcc tacccgcagc ctgcatcgtt tgcccaaacc
     1921 atcccgcaat acggcaccag gcggcaaatc ggcggctatc tcgccacccg tttccgcgcc
     1981 gccgacaacc tttcgctgat actcggcggc agatacagcc gttaccgcac cggcagctac
     2041 gacagccgca cacaaggcat gacctatgtg tccgccaacc gtttcacccc ctacacaggc
     2101 atcgtgttcg acctgaccgg caacctgtct ctttacggct cgtacagcag cctgttcgtc
     2161 ccgcaatcgc aaaaagacga acacggcagc tacctgaaac ccgtaaccgg caacaatctg
     2221 gaagccggca tcaaaggcga atggcttgaa ggccgtctga acgcatccgc cgccgtgtac
     2281 cgcgcccgta aaaacaacct cgccaccgca gcaggacgcg acccgagcgg caacacctac
     2341 taccgcgccg ccaaccaagc caaaacccac ggctgggaaa tcgaagtcgg cggccgcatc
     2401 acgcccgaat ggcagataca ggcaggttac agccaaagca aaacccgcga ccaagacggc
     2461 agccgcctga accccgacag cgtacccgaa cgcagcttca aactcttcac tgcctaccac
     2521 tttgcccccg aagcccccag cggctggacc atcggcgcag gcgtgcgctg gcagagcgaa
     2581 acccacaccg accctgccac gctccgcatc cccaaccccg ccgccaaagc ccgcgccgcc
     2641 gacaacagcc gccaaaaagc ctacgccgtc gccgacatca tggcgcgtta ccgcttcaat
     2701 ccgcgcgccg aactgtcgct gaacgtggac aacctgttca acaaacacta ccgcacccag
     2761 cctgaccgcc acagttacgg cgcactgcgg acagtgaacg cggcgtttac ctatcggttt
     2821 aaataaggtc gtctgaaaac ggagtttctg cgaagctata gtggattaac aaaaaccagt
     2881 ac
//
LOCUS       DAWFHY010000137         2858 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.137, whole genome shotgun sequence.
ACCESSION   DAWFHY010000137 DAWFHY010000000
VERSION     DAWFHY010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2858
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.137"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..457)
                     /locus_tag="WGQ68_002000"
     CDS             complement(<1..457)
                     /locus_tag="WGQ68_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fhaB protein"
                     /protein_id="HEZ0715732.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PFGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYTQFDVGNRGAILNNSRSNTQTQLGGWIQGN"
     gene            613..771
                     /locus_tag="WGQ68_002001"
                     /pseudo
     CDS             613..771
                     /locus_tag="WGQ68_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1260..1493
                     /locus_tag="WGQ68_002002"
                     /pseudo
     CDS             1260..1493
                     /locus_tag="WGQ68_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1421..2005)
                     /locus_tag="WGQ68_002003"
     CDS             complement(1421..2005)
                     /locus_tag="WGQ68_002003"
                     /inference="COORDINATES: protein motif:HMM:NF013235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEZ0715733.1"
                     /translation="MASIILNYLEKDGRPVIHGMGYISEWKINNGKVFYNISDTILNE
                     LLLKSGRSMFLLMDWSVFNSLPGKYCGILYKLCKDYAGIGKTPYFTVQEFRHYLGLND
                     KEYEEFRALKAKIIKPAIEHINNSENVDIKIIPQLNQNMADEDIETAFSESKIEPLAT
                     LIKFLETKTPRSSSGLERLCHCPSRTALNMDNSG"
     gene            complement(2440..>2858)
                     /locus_tag="WGQ68_002004"
     CDS             complement(2440..>2858)
                     /locus_tag="WGQ68_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0715734.1"
                     /translation="DFRTGSVGVTLLDGSRHAPNPVKEIEVARVLQNIGLQSGSTTEA
                     AVRFMLYCMRQQVFWDGNKRTATLFANGLMMERGRGILEISEMQMPQFNEKLSAFYRS
                     GDDTDISKFVYQNCISGIDYFGADEDIDFPDSPNID"
BASE COUNT          792 a          620 c          559 g          887 t
ORIGIN      
        1 gattgccttg aatccaaccg cctagctgtg tttgggtgtt gctgcggctg ttgtttaaaa
       61 tcgccccgcg attacccaca tcaaactgtg tatattgatt aacagaaacc cctgccgaag
      121 taggggtttg aatattgact tgcggtatgc cgttacctgt ttgcagaatc gtggcttgtt
      181 gggttttagg agcagcttta tcagcaataa tgccatcagc aaaagcaata ttggccgtac
      241 ctacagccaa acataaagaa aagcccaata aagaaaaaga aaagatattt gaacgacaaa
      301 caggtgcatg agtagtacca aaaggaacag atttcacatg agcgctgcct gaatcactat
      361 cggcacagct tttaccttcg cgcttggtag tttcagcaac ggctaccaca gccccacgtt
      421 tgcggttgaa aattacacga tagagagttt tattcatgat ttcagttatt tgatttttat
      481 agagttatta gaaaaaattg gatagtctga ccattctaga tcaaggattt tggcgagtca
      541 attaccgcca ttttactgcc atttgtttat taaattaggg actttactag ataacggtta
      601 aaaatcccat tcgaacgaaa tggcaaggtt tataccgtcg ttgtccctaa tgcgcaatca
      661 gcaacattat tgccgattat ccgagagaaa gttaagtctg atggcattgt gtatacggat
      721 acctttcata gttatgatgt acttgatgtc agtgaattta gccatttacg caagttttcc
      781 agtatttgac tggcaattta aaacaatcgg aagttgtccc atttccagta tttgactggc
      841 aatttaaaac agtcggattt tgtcccattt gttggccaag tctttacttg cttggccgtt
      901 tgaatttaaa aagcagtctt tctactttcc gacctttttt ctgttgtaag gtctatgatc
      961 caatagcatt cccaaagagc attttggacg gtatttaaag gaatgtgaat agcgttttaa
     1021 caacagtgaa atagaatcta aaattcagat ttgaagacaa ttagttaatg ggaatttggt
     1081 ctggttatct agtacagcct ctaaaatttt agtgaccgaa caaactcgaa aaatccacat
     1141 cgccgatgcg ttccagccgg tcggcaaagc tgtcaagatt cacagattca gcgtttgaga
     1201 cctttgcaaa attccccaaa atcccctaaa ttcccaccaa gacatttagg agatttctca
     1261 tgagcacctt cttccagcaa accgcacaag ccatgattgc caaacacatc gaccgtttcc
     1321 cgctattgaa gttggaccag gtgattgatt ggcagccgat cgaacaatac ctgaatcgtc
     1381 aaagaacccg ttaccttaga gaccaccgcg gccgtcccgc ctacccgctg ttgtccatgt
     1441 tcaaagccgt tctgctcgga caatggcaca gcctctccaa tcccgaactc gaacgtggtg
     1501 ttttggtttc cagaaattta atcagtgtgg ctagcggctc tattttcgat tcagaaaagg
     1561 ctgtttctat atcctcgtct gccatgttct ggtttaattg cggtataatt ttgatgtcca
     1621 cattttcact gttgttgata tgctctattg ccggtttaat tattttggcc ttcaacgctc
     1681 taaattcttc gtattctttg tcattcaatc ccaaatagtg tctaaattct tgaacggtaa
     1741 aatagggggt tttgccgatg cctgcgtaat ccttgcacaa tttgtatagg attccgcaat
     1801 attttcccgg taagctattg aacacactcc aatccatcag caaaaacatg cttcttccgc
     1861 ttttcaatag taactcatta agaatggtgt ctgatatatt gtagaaaact ttgccattgt
     1921 tgatcttcca ctctgaaata tagcccatac cgtgtatgac gggacggccg tctttctcca
     1981 aataattcaa aatgattgat gccattcgta gagcatccaa gtctttttta gttgttttct
     2041 ttgaagttga tatccaatac aacgttccag atctaagata tctatcgcaa caaaaccgtt
     2101 gtcatcataa gtaccacctt attcaaaatg tgttttaatc ttctgcaaaa aaagatacca
     2161 atacttgtat tgcatttcag tcaaggaatg gcccatgtgg attaaggatt gcggtgtttt
     2221 ttgtttatca atatggtttg atgtcatttt gatttccatt tcatgtggct actatatctc
     2281 tctattatgc actaatacac agagtttctt gatgttagaa cgaatacggc gtccgtgtcg
     2341 gcgtgaaatt ctgattttcc ttatacgaaa ccgcaaacct ttattacatc tcctgaataa
     2401 tttaggatac tttttaggta ttgaaaaaca ccttggtttt tagtctatgt tcggtgagtc
     2461 ggggaaatct atatcttcgt ccgcgccgaa atagtctatg cccgatatac aattttgata
     2521 cacaaacttg gaaatatcgg tatcgtcacc cgagcgatag aatgcggaca gtttttcatt
     2581 gaattgcggc atctgcattt cggagatttc caagatgcca cggcccctct ccatcatcag
     2641 accattggca aataaggttg ccgttcgttt gttgccgtcc caaaaaacct gctgccgcat
     2701 acaataaagc ataaaacgga cggctgcctc cgtcgtcgaa ccgctttgca gtccgatatt
     2761 ttgtaacacg cgagccactt caatttcctt caccggattt ggggcatgac gggaaccgtc
     2821 caatagcgtt acgccgaccg aaccggtacg gaaatcac
//
LOCUS       DAWFHY010000138         2726 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.138, whole genome shotgun sequence.
ACCESSION   DAWFHY010000138 DAWFHY010000000
VERSION     DAWFHY010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.138"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(123..1079)
                     /locus_tag="WGQ68_002005"
                     /pseudo
     CDS             complement(123..1079)
                     /locus_tag="WGQ68_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227655.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1315..2280
                     /locus_tag="WGQ68_002006"
     CDS             1315..2280
                     /locus_tag="WGQ68_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014574230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEZ0715735.1"
                     /translation="MKPRFYWAACAVLLTACSPEPAAEKTVSAASASAATLTVPTARG
                     DAVVPKNPERVAVYDWAALDTLTELGVNVGATTAPVRVDYLQPAFDKAATVGTLFEPD
                     YEALHRYNPQLVITGGPGAEAYEQLAKNATTIDLTVDNGNIRTSGEKQMETLARIFGK
                     EARAAELKAQIDALFAQTREAAKGKGRGLVLSVTGNKVSAFGTQSRLASWIHGDIGLP
                     PVDESLRNEGHGQPVSFEYIKEKNPDWIFIIDRTAAIGQEGPAAVEVLDNALVRGTNA
                     WKRKQIIVMPAANYIVAGGSRQLIQAAEQLKEAFEKAEPVAAGKE"
BASE COUNT          591 a          728 c          844 g          563 t
ORIGIN      
        1 gaaaggccgt atgctaccgc atactggcct ttttctgtta gggaaagttg cacttcaaat
       61 gcgaatccgc cgaccttttt taatggttgt ttcaaatcaa aatatctatg ccgccggccc
      121 catcagcact tcttcacatc cgaaggcaaa aatccgtaat gccgtctgaa cgcttcgttg
      181 aaccgtcccg cgtggcggta gccgcaaaag tgcatggcgg cttggacggt gctgccggat
      241 tcgatgaggg cgagcgcgtg ttccagccgc aggcggcgca ggcatccggc gacggtttcg
      301 ccggtttgcg ctttgaaata gcgtttcagg tagcattcgt tcagtccgac gcggcgggcg
      361 atttcggcga tggtcagcgg acgggcgaat tcgtgttgca ggatgtcggc ggcttcgtct
      421 atgcgccgac ggcggtaacc gttgtcgtgg cggcggaagg tgaagcgcaa taatcgggcg
      481 gagagttcca gcgcggcggc ttcgtcggca agcaggccga agccgtccga ttcgaacggg
      541 cgttgcagca gtgggcaggc cgccgccgtc agtgccgctg cgttttgcgc cagccgttac
      601 agggcgaatc ggcctatcgt ttgcggcgaa aacaggcgtt cgtccagcaa gccttcgtcg
      661 tgccagcggc gcagtttttc cagcgaaaaa tccaaatgca gcgcgcacat gccgctgttg
      721 tcgggcagca gggtttcgga tacgtccgcc aactcgccgc gtaccagcca gatttcgccg
      781 gcagacgggc ggtattccct gccgcccatt tgtaaccggt tctgccccga caccatgacg
      841 aacaaggcgc agttgtggct gaaattgtgg atttcggtgg gaaacgcgcc cgttccgccg
      901 ccgcgcatcc gcgacagggt gatgcccgag tcgaagcggt tgatgcacat ttccagatgc
      961 aaaccgggct gttttgcctg cgcaatgagc gcgctgtcgg aacagccgtc caacgcccag
     1021 ccggatttat cggagcggac ataggtttgg tactggcggt agatggcggc ggtgttcatg
     1081 attggatagg aacgagttgt ctaacaaatg aattaaatag gaattattac caataatcaa
     1141 gcgcagggat tggttgaaac ggaaaaggtc gtctgaaagg gtgtttcaga cgaccttttc
     1201 cgtatcggga atttgttttg ccgtatcggg aattttgcgt tttgcggcgt ggtttctgca
     1261 ggttgtttgc ttaataataa acattcttat tcgtatgcaa aggaaccgca caccgtgaaa
     1321 ccgcgttttt attgggcagc ctgcgccgtc ctgctgaccg cctgttcgcc cgaacctgcc
     1381 gccgaaaaaa ctgtatccgc cgcatccgca tctgccgcca cactgaccgt gccgaccgcg
     1441 cggggcgatg ccgttgtgcc gaagaatccc gaacgcgtcg ccgtgtacga ctgggcggcg
     1501 ttggatacgc tgaccgaatt gggcgtgaat gtgggcgcaa ccaccgcgcc ggtgcgcgtg
     1561 gattatttgc agcctgcatt tgacaaggcg gcaacggtgg ggacgctgtt cgagcccgat
     1621 tacgaagccc tgcaccgcta caatcctcag cttgtcatta ccggcgggcc gggcgcggaa
     1681 gcatatgaac agttggcgaa aaacgcgacc accatagatc tgacggtgga caacggcaat
     1741 atccgcacca gcggcgaaaa gcagatggag accttggcgc ggattttcgg caaggaagcg
     1801 cgcgcggcgg aattgaaggc gcagattgac gcgctgttcg cccaaacgcg cgaagccgcc
     1861 aaaggcaaag gacgcgggct ggtgctgtcg gttacgggca acaaggtgtc cgccttcggc
     1921 acgcagtcgc ggttggcaag ttggatacac ggcgacatcg gcctaccgcc tgtagacgaa
     1981 tctttacgca acgaggggca cgggcagcct gtttccttcg aatacatcaa agagaaaaac
     2041 cccgattgga ttttcatcat cgaccgtacc gccgccatcg ggcaggaagg gccggcggct
     2101 gtcgaagtat tggataacgc gctggtacgc ggcacgaacg cttggaagcg caagcaaatc
     2161 atcgtcatgc ctgccgcgaa ctacattgtc gcgggcggct cgcggcagtt gattcaggcg
     2221 gcggagcagt tgaaggaggc gtttgaaaag gcagaacccg ttgcggcggg gaaagagtag
     2281 ggagtcgtct gaaaacggag cttccgaagg aagcgggggg tttctgcgaa gctaaagtgc
     2341 ggtttcaacg aattgaaaag cagcctgtat gttgaaaata ccgctcaagc aaacctgcgg
     2401 tttgccgccc tctccctagc cctctcccac agggagaggg gattgggttg caggctgcct
     2461 ttaaggttta ggcaaatttt taacttcgtt gaagctccgt tttcagacga ccttttggag
     2521 taccgcaggc acacgcatcg aacggctgaa tcaaagttca gaccgatgac agcccgcacc
     2581 cgagtttatg cggcaaacag cgaggctacg gcaacccgtc ccctctccct gtgggagagg
     2641 gttagggaga gggcagtaag ccgtaggctt acatcaaagt cgataacgct ttcacaaagc
     2701 ctgtgtgcac tgaaaactga aaggtc
//
LOCUS       DAWFHY010000139         2686 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.139, whole genome shotgun sequence.
ACCESSION   DAWFHY010000139 DAWFHY010000000
VERSION     DAWFHY010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.139"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(290..499)
                     /locus_tag="WGQ68_002007"
     CDS             complement(290..499)
                     /locus_tag="WGQ68_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715736.1"
                     /translation="MNYFGMVEFLRLMAMVRPPFVFFSGTRSELPAYLDLVAELRLTG
                     WERFAGSQTLTVSSTSTAIQATTTT"
     gene            complement(637..792)
                     /locus_tag="WGQ68_002008"
     CDS             complement(637..792)
                     /locus_tag="WGQ68_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715737.1"
                     /translation="MDAKHRLKKYVYHLLVAIDQLFNALTGGAADETLSSRTYRGARL
                     AQKPKTR"
     gene            complement(771..1376)
                     /locus_tag="WGQ68_002009"
     CDS             complement(771..1376)
                     /locus_tag="WGQ68_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715738.1"
                     /translation="MTIYFKNGFYDDTLGGISEGAVAVRAEEYAALLAGQAQGGQIAA
                     DSDGRPVLTPPRPSEYHEWDGKKWEIGEAAAAARFAEQKTATAFRLAAKADELKNSLL
                     AGYPQVEIDSFYRQEKEALARQADNNAPTPMLAQIAATRGVELDVLIEKVIEKSARLA
                     VAAGAIIGKRQQLEDKLNTIETAPGLDALEKEIEEWTLNIG"
     gene            1456..1743
                     /locus_tag="WGQ68_002010"
     CDS             1456..1743
                     /locus_tag="WGQ68_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HEZ0715739.1"
                     /translation="MKIEFDSEKNQRNIEERNLPFESVGQIRWTTAVIVPDVCFDYPE
                     PRYVAAAYLGDTQRLHIVCFTPIKDGIRVISFRKANKREVKKYATSILNKR"
     gene            1715..2017
                     /locus_tag="WGQ68_002011"
     CDS             1715..2017
                     /locus_tag="WGQ68_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HEZ0715740.1"
                     /translation="MQRQSLTNADGEVRELAAEDFALARPIAEALPEDLAQVLFSHQK
                     QLEEKGVMQKRNTGKTPKQLVTIRLSADVVEKFRAGGKGWQTRINEVLRQYVAQLK"
BASE COUNT          526 a          693 c          771 g          696 t
ORIGIN      
        1 aatgttatta ccttgatgag gtagtgtcaa ttttgaaaaa gattgcctga cagtttgtcc
       61 gatttcaaaa tctccgcgac aagcatgttt taaagccatt cggggatttg ggggcggatt
      121 gtaaggcggc ggcagctttt gcacgaattg gcggcagctt ttgcaccgcc gaagcgtagg
      181 aaattgtatt agcttttgca caaatttatc cccgtttata tggtctgtta aacggggatt
      241 atcttatgcc tttaaaagta gtacgctact tgcggtcaga acttataaat caggtggtcg
      301 tcgtagcttg aattgctgtt gatgtgctgc tcacagtcaa ggtttggctg cctgcaaaac
      361 gctcccatcc ggtcaggcgc agttctgcca cgaggtcaag ataagcaggc agttcgctgc
      421 gggtgccgga gaagaagaca aatggcggcc gcaccattgc catcaggcgc agaaactcta
      481 ccatgccgaa gtagttcatc gcgtcccctt ttccccggcg gcgcggcttt ggttgaaccg
      541 cgcgtcgtgc tgcctgcctt tcagttcgct gatatacgcc tgccgacagt gttggcggtc
      601 gaaaaacacg ccgttaatca gggtatataa aatctttcag cgggttttgg gcttttgggc
      661 gagccgcgcg ccgcgatagg tgcggcttga gagggtttcg tccgccgcgc cgccggttag
      721 ggcgttgaac agttggtcga tggctaccaa caggtggtaa acgtattttt tcagccgatg
      781 tttagcgtcc attcttcgat ttccttttcc agcgcgtcca atccgggcgc ggtttcgatg
      841 gtgttcaatt tgtcttcgag ctgctgacgc tttccgataa tcgcgccggc ggcaacagcc
      901 aggcgggcgg atttttcgat aactttttca atcaaaacgt ccaattccac gccccttgtg
      961 gcggcgattt gcgccagcat cggggtcggg gcgttgttgt ccgcctgccg cgcgagggct
     1021 tctttttcct gcctgtaaaa gctgtcgatt tccacttggg gatagcccgc caagaggctg
     1081 tttttgagtt cgtccgcctt tgccgcgagg cggaatgccg tggcggtttt ttgttcggcg
     1141 aaacgggcgg cggcagcggc ttcgccgatt tcccatttct tgccgtccca ttcgtggtat
     1201 tcagacgggc gcggcggggt taaaacgggg cgaccgtcgg aatctgcggc aatctgcccg
     1261 ccctgcgcct gtcctgccaa aagggcggcg tattcttcgg cgcggacggc aaccgcgcct
     1321 tcggagatgc cgcccaatgt gtcgtcgtaa aagccgtttt tgaaatagat ggtcatttcg
     1381 gggtttcctt ttttgtattt acattttggt tttttgccgc ttataatgta acgacaaaat
     1441 acgctgcaag ctgcaatgaa gattgaattt gactccgaaa aaaatcaacg caatatcgaa
     1501 gagcggaatt tgccctttga atcagtcggg caaatacgct ggacaacggc cgttattgtg
     1561 cccgatgtct gctttgatta ccccgaaccg cgttatgttg ccgctgctta tctaggcgat
     1621 acgcaacgcc tgcacattgt ctgttttacg ccgataaaag acggcatccg tgtcatcagt
     1681 tttcgcaaag cgaataaaag agaggttaaa aaatatgcaa cgtcaatcct taacaaacgc
     1741 tgacggcgaa gtgcgggaac ttgccgccga agattttgcg cttgcccgcc ccattgccga
     1801 agccctgccc gaagacttgg cacaggtgct tttttcccat caaaaacaat tggaagaaaa
     1861 gggcgtaatg caaaaacgaa acacaggcaa aaccccgaaa caactcgtta ccatacgcct
     1921 gtccgccgat gtggtcgaaa aattccgcgc cggcggcaag ggctggcaga cgcgcatcaa
     1981 cgaggttttg cgccaatatg tcgcccaatt gaaataacgg gggcggccgc tgcttatcgg
     2041 gcgcgcccga ctatcctttc ccgcgcggtt tcggccgtgc ggttttttgt tgccggtgcc
     2101 gtccgaaggg ggcagcggct ttttgcgcgg tcattcccgc ctttcgttat tcccgccact
     2161 ttccgtcatt cccgcgaagg cgggaatcca gtccgttcgg tttcagccgt ttccgataga
     2221 ttcctgccgc gtttggggtc tggattcccg cctgcgcggg aatgacggcg gcgggggttt
     2281 ctgttttttc cgatagattc ctgttgcgtc ggggtctgga ttcccgcttt cgcgggaatg
     2341 acggcggcgg gggtttctgt tttttccgat agattcctgc cgcgtttggg gtctagattc
     2401 ccgctttcgc gggaatgacg gtggcggggg tttctgtttt ttccgataga ttcctgccgc
     2461 gtttaggggc tggattcccg ctttcgcggg aatgacgaaa gcggcgggaa tgacgaaagc
     2521 ggcgggaatg acgaagagtg gcaggattgg cggggcatag gtttcggggg attggcggcg
     2581 cggccggttt cggtcggatt gcttggatcg gcgggctgaa gcccatctct acagcccatc
     2641 tctacagccc atctctacaa cccatctcta caacccatct ctacag
//
LOCUS       DAWFHY010000140         2686 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.140, whole genome shotgun sequence.
ACCESSION   DAWFHY010000140 DAWFHY010000000
VERSION     DAWFHY010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.140"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            59..1432
                     /locus_tag="WGQ68_002012"
     CDS             59..1432
                     /locus_tag="WGQ68_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEZ0715741.1"
                     /translation="MSNPFSSLGLGTELVSALTAQGYENPTPIQAAAIPKALAGHDLL
                     AAAQTGTGKTAAFMLPSLERLKRYATASTSPAMHPVRMLVLTPTRELADQIDQNVQSY
                     IKNLPLRHTVLFGGMNMDKQTADLRAGCEIVVATVGRLLDHVKQKNIHLNKVEIVVLD
                     EADRMLDMGFIDDIRKIMQMLPRQRQTLLFSATFSAPIRKLAQDFMNAPETVEVAAQN
                     TTNANVEQHIIAVDTIQKRNLLERLIVDLHMNQVIVFCKTKQSVDRVTRELVRRNLSA
                     QAIHGDRSQQSRLETLNAFKDGNLRVLVATDIAARGLDIAELPFVINYEMPAQPEDYV
                     HRIGRTGRAGADGVAISLMDESEQKMFESIKELTGNKLLIERIEGFEPQWWEQGGAKP
                     EKPEMREPRQRNRYESAKAQREKNTRPENAANDAGAACGKIAGRSRRSRREHRTCALL
                     QPRYGVK"
     gene            1737..>2686
                     /locus_tag="WGQ68_002013"
     CDS             1737..>2686
                     /locus_tag="WGQ68_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002235001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 7-halogenase"
                     /protein_id="HEZ0715742.1"
                     /translation="MLEKQHFPRFVIGESLLPHCMEMLEEAGFADAVRAEPGFQLKNG
                     AAFSWGSRYTEFDFTDKFSDGPGTTYQVRRAVFDKILIEEAAKQGVEVRFGHGVTAFD
                     NSGDFARLNIETDTGKRYELTAKFVLDASGYGRVLPRLLNLETPSHLPPRQAHFTHID
                     DNITNPKFDRNKILITTYPQHRDVWIWLIPFGDNRCSAGVVGTPDKLVGESETVLKKF
                     VYECPMLNEILDKAVWENDFPFRSIQGYSANVKSLHGRHFALLGNAAEFLDPVFSSGV
                     TIALHSAKLAADLLAKQLKGEAADWQTEFAEPLMIGVDAFR"
BASE COUNT          670 a          840 c          674 g          502 t
ORIGIN      
        1 cccatcctat ctgtttcccg cttcagacgg cataaggtct gaaagaaaga ctacaattat
       61 gagtaatcca ttttcctctt taggtttggg tacggaactc gtttccgcac tgaccgcgca
      121 aggttacgaa aacccgacgc ccatccaagc cgccgccatt cccaaagcac tcgccggtca
      181 tgatttacta gccgccgcgc aaaccggcac gggcaaaacc gccgccttta tgctgccctc
      241 gctcgaacgc ctcaaacgtt acgccaccgc cagcacctcg cccgcgatgc accccgtgcg
      301 tatgctcgtg ttgaccccca cgcgcgaact cgccgaccaa atcgaccaaa acgtgcagtc
      361 ctacatcaaa aacctgccgc tgcgccacac cgtcttgttc ggcggtatga atatggacaa
      421 acagaccgcc gatttgcgtg ccggctgcga aatcgtcgtc gccacagtcg gacggctgct
      481 cgaccacgtg aaacagaaaa acatccattt gaacaaagtc gaaatcgtcg ttttggacga
      541 agccgaccgt atgctggata tgggttttat cgacgacatc cgcaaaatca tgcagatgct
      601 gccccgccaa cgccaaaccc tgctcttttc cgccaccttc tccgccccga tacgcaaact
      661 ggcgcaagac ttcatgaacg cgcccgaaac cgtcgaagtc gccgcgcaaa acaccaccaa
      721 cgccaacgtc gagcagcaca tcatcgccgt cgataccatt cagaagcgca acctgctcga
      781 acggctgatt gtcgatttgc atatgaacca ggtcatcgtg ttctgcaaaa ccaaacaaag
      841 cgtcgaccgc gtaacgcgcg aactggtgcg ccgcaacctg tccgcacagg cgatacacgg
      901 cgaccgttcc caacaaagcc ggctcgaaac actcaacgcc ttcaaagacg gcaacctgcg
      961 cgtcctcgtc gccaccgata tcgccgcgcg ggggctggat attgccgaac tgcccttcgt
     1021 catcaattac gaaatgcccg cccagcccga agactacgtc caccgcatcg ggcgcacggg
     1081 gcgcgcgggc gcggacggcg tggcgatttc cctgatggac gaatccgaac agaaaatgtt
     1141 tgaatccatt aaagagctga ccggcaacaa gctgctcatc gagcgcatcg agggcttcga
     1201 gccgcaatgg tgggaacagg gcggcgcaaa accggaaaaa cccgaaatgc gcgaaccgag
     1261 acaacgcaac cgctacgaat ccgccaaagc gcaacgcgaa aaaaacaccc ggccggaaaa
     1321 tgcggcaaac gatgcgggcg cggcttgcgg aaaaattgcc ggacgcagcc gccgaagccg
     1381 ccgggaacac cggacgtgcg ccctgctcca accgcgttac ggcgtaaaat agccctgaaa
     1441 atcaaatgcc gtctgaacat ttcccgtttc agacggcatt tttcaaaccg gactgacgca
     1501 tcgggagcaa ccgcccgcac cggataaatt tctgccgcaa acagtttcag acggcatttg
     1561 ccgcctgtac aatatagtgg attaacaaaa attaggacaa ggcggcgagc cgcagacagt
     1621 acaaatagta cggaaccgat tcacttggtg cttcagcacc ttagagaatc gttctctttg
     1681 agctaaggcg aggcaacgcc gtactgattt ttgttaatcc actatatcaa gtctgcgtgt
     1741 tggaaaaaca gcactttccg cgcttcgtca tcggcgaaag cctgctgccg cactgtatgg
     1801 aaatgctgga agaagccgga tttgccgatg ccgtgcgcgc cgagcccggc tttcagttga
     1861 aaaacggcgc ggcgttttcg tggggcagcc gttataccga attcgatttc accgataaat
     1921 tttcagacgg ccccggcacg acttatcaag tgcgccgcgc cgtgttcgat aaaatcctga
     1981 ttgaagaagc cgccaaacaa ggcgttgaag tacgtttcgg gcacggcgta accgcgttcg
     2041 acaacagcgg cgattttgcc cgcctgaaca tcgaaaccga caccggcaag cgctatgaac
     2101 tgaccgcgaa attcgtcttg gacgcaagcg gttacggacg cgtgctgccg cgcctgctaa
     2161 acttggaaac gccctcgcac ctgccgccgc gccaagcgca tttcacgcac atcgacgaca
     2221 acatcaccaa cccgaaattc gaccgcaaca aaatcctgat taccacctat ccgcaacacc
     2281 gcgacgtgtg gatttggctg attcccttcg gcgacaaccg ctgttccgcc ggcgtggtcg
     2341 gcacgcccga caaacttgtc ggcgaatcgg aaacggtgtt gaaaaaattt gtttacgaat
     2401 gcccgatgct gaacgaaatt ttggacaaag ccgtttggga aaacgatttt ccgttccgct
     2461 ccatccaagg ctattccgcc aatgtgaaat cgctgcacgg caggcatttc gcgctgttgg
     2521 gcaatgccgc cgagttcctc gatcccgtgt tctcgtcggg cgtaaccatc gcgctgcact
     2581 ccgccaaact tgccgccgat ctgttggcaa aacaactcaa aggcgaagcc gccgattggc
     2641 aaaccgaatt tgccgaaccc ttgatgatcg gcgtggacgc gttccg
//
LOCUS       DAWFHY010000141         2654 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.141, whole genome shotgun sequence.
ACCESSION   DAWFHY010000141 DAWFHY010000000
VERSION     DAWFHY010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.141"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(92..1435)
                     /locus_tag="WGQ68_002014"
                     /pseudo
     CDS             complement(92..1435)
                     /locus_tag="WGQ68_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
     gene            complement(1542..1805)
                     /locus_tag="WGQ68_002015"
     CDS             complement(1542..1805)
                     /locus_tag="WGQ68_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002234239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715743.1"
                     /translation="MDKEIKICPRCEQGYLYHAKPKYFSGEVILCDECYAMWLGDMKI
                     FYGQYGKDFYDYHEFMKDKGIEEINMWEGELFDHPYYEDEKFK"
     gene            complement(1824..2555)
                     /locus_tag="WGQ68_002016"
     CDS             complement(1824..2555)
                     /locus_tag="WGQ68_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002225601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ0715744.1"
                     /translation="MSATEIEKAKAKITAYAKLVAGATVGVTGGNVDVAANASETAVK
                     NNALDIIWDIGNLVWDGGKWIYAKSIGDKQMAREAAIDFGVDAAAAAVPFVPAGATKI
                     SRGRAYVLKAGDEAVDTAKAIQEIQKQTGIKLTYDKVNKVWTTPAGLDYGLDAKHGNR
                     IKHVLAHTIPNPNKPVHSVFNVSRKEVLPLVDEAWRMKGNPLPNDSSVYLVDMKKPIG
                     TKGETKVRIVVQKGTNKIISAYPQK"
BASE COUNT          598 a          696 c          546 g          814 t
ORIGIN      
        1 aaacttctcg atattcttca ggattttcac gtatccattt ttgttgttcc ttttgaaatt
       61 catcacactt tgcaccaacg catctaggat acaaagtatt attctccacc gctatctgtg
      121 ccgcattcgc cgctgtattc acatccccgc ctacaacggc agacgcagtg ccggcaacca
      181 gttttgaata ggcagtaatc ttcgctttag ctttttcgat ttcggtcgca ctcatacgac
      241 tgaaatcagt attcttaacc aaagcctcac caacaatctc accgaccgcc gctcctatcg
      301 caccatcctg acacttgccc ttattcgccg ccgcagccgc acagcccgct atggcatggg
      361 caatcttgtg gactatgtag tgctgatcca actgtttgat tttactggct gcttctccat
      421 gcgcggtatt gaccaaagcc gcaaggatat tcgcttccag attgtctttc aggctgccgc
      481 cgttaacagt ggtattaatc agcgcggcac tgcccgcatt ggccaggttg acggttaggt
      541 cattgaccca ctgcttatcg ctgatattgt tcagtgccga agcgccgatt ttgtcggcta
      601 cgcctgcggt agcggcggca accaccagat ttttcaccgt gcggcttctg cccagctctt
      661 tcagggtgtt accgatattg cctttgttgt tgatcaggga aatggaagcc tggctcacca
      721 atgcggtaaa ggcagcatcg gccacggctg ccgatgcggt accctgtgcc gctccgataa
      781 ggcctgcgcc tactcctccg gttaccgccg ctactgctag ggcgataatt gcggctccgg
      841 ctccggttag gccttcctgt ttatagtccc atttgtcgta agcgagctgt acttggttcc
      901 agttcacgtc cttgaccgtc tgaagctgtt tcagataggc atattcgggc tgtttggcca
      961 gcttttcgat ttcggttttg aggttgcctt tggggatgtc ggcgatatag ccgccgggag
     1021 cggtcagctt aggcagtgcc ggcccttcaa agctcggtag cttcagcgtt tcaaccgtgc
     1081 tgccgcttcc ggcctgcttt tgccatacgg tcgagttgga ttccagcttt tcttcggttt
     1141 ggatgcggtt aacgatgcct tttaggataa ttttcgcatc ggctcgggct ttttcaccca
     1201 cccctgcctg tatgtcggct ccggaaaggg tggttttgaa ttcggtgcct tcgagtacgg
     1261 tatcccagcc ggaacgggtt ttggctgttt gggcgataac gcgtacgggc agtttggttt
     1321 cgttcagctc gtttttgctg taattgcttt tacccacttt gatgccgatg aaacgggtac
     1381 ttttctgaac attcaattgg tgttggtgaa tgccttcggc tgccagcagt tgcattgagg
     1441 ccgtctgaaa aacggatatt cagacggcct attgctactt taaataatat ttgggtttaa
     1501 aatatcatcg tatttcccaa attcggggaa cagaaaatca attatttaaa tttttcatcc
     1561 tcataatatg ggtgatcaaa aagctctcct tcccacatat ttatttcttc tatgccttta
     1621 tctttcataa actcatgata atcataaaaa tcttttccat attgtccgta aaaaattttc
     1681 atatccccaa gccacatagc ataacattca tcgcataaaa tgacctcccc agagaaatat
     1741 ttaggctttg catgataaag gtagccttgc tcacatcttg ggcaaatttt aatttcttta
     1801 tccataagag attcctttct taattatttc tgaggatatg cagaaatgat tttatttgtt
     1861 cctttttgca caacaatccg cacttttgtt tctccttttg ttccaatagg tttcttcata
     1921 tctacaagat atacggatga atcatttggc aaaggatttc ctttcattct ccaagcttca
     1981 tcaaccaaag gcaaaacttc tttacgggac acattaaaaa cagaatgaac aggtttgttt
     2041 ggatttggaa ttgtatgggc taaaacatgt ttaatcctat taccatgctt agcatctaac
     2101 ccataatcta accccgccgg tgttgtccaa accttattaa ccttatcata agtaagcttg
     2161 attccggtct gcttctgaat ttcctgtatg gctttagccg tatcaactgc ttcgtctccc
     2221 gccttcagaa cataagccct gcctcggctg attttagtcg cacctgccgg aacaaaggga
     2281 acggcagctg cggcggcatc cacaccaaaa tcaatcgccg cttctcgagc catctgctta
     2341 tcgccaatag atttggcgta aatccattta ccgccgtccc atacgaggtt gccgatatcc
     2401 caaataatat ctaatgcatt atttttaaca gctgtttcgg aagcatttgc cgccacatca
     2461 acattgcctc ctgtaacacc tacagtcgct ccggctacca attttgcata cgcagtaatc
     2521 ttcgctttag ctttttcgat ttcggtcgca ctcatacgac tgaaatcagt attcttaacc
     2581 aaagcctcac caacaatctc accgactgca gcgcctatcg caccatcctg acacttgccc
     2641 ttattcgccg ccgc
//
LOCUS       DAWFHY010000142         2562 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.142, whole genome shotgun sequence.
ACCESSION   DAWFHY010000142 DAWFHY010000000
VERSION     DAWFHY010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.142"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(491..862)
                     /locus_tag="WGQ68_002017"
     CDS             complement(491..862)
                     /locus_tag="WGQ68_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715745.1"
                     /translation="MRFYIDNKECDYIFFRKAVNISDDFIQSGFITPEGLITKNSNPK
                     LFNQYSKMVSKNKCQYEMVTFLSDEMKNIIELLSNDDAYIEFAYSEDFYVLPEIKIDK
                     RTLKSLNFYIDFGVKYIINKI"
     gene            complement(996..1439)
                     /locus_tag="WGQ68_002018"
     CDS             complement(996..1439)
                     /locus_tag="WGQ68_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715746.1"
                     /translation="MKKQITEATMMLSIIAPAMANGLDNQAFEDQVFHTRADAPMQLA
                     ELSQKEMKETEGAAAPLVAIGILHAGRFLAQRWVTQRVAAQALSRGANVYARTSQQAR
                     AVANQAWGRQNVIRHGQKEIHSNYSHFQNSSQKIRGHAFYGNKHR"
     gene            complement(1527..1751)
                     /locus_tag="WGQ68_002019"
     CDS             complement(1527..1751)
                     /locus_tag="WGQ68_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715747.1"
                     /translation="MMKNKYILIKAAIGITAISIFYLLTQESSGKTEEPSYFLMFMFL
                     NLLWFEENKTVMAAVTAMIAAHFIFVALSD"
     gene            complement(2176..2463)
                     /locus_tag="WGQ68_002020"
     CDS             complement(2176..2463)
                     /locus_tag="WGQ68_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002217501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715748.1"
                     /translation="MDDLIAAFLGYLSGKYREGKISRLGISLTAGSIFFAVFFIHELI
                     IFIVDGNKENLWNFITEMMKDLFLFSCSVCLLFYFLLFIAETIGKTINKIK"
BASE COUNT          848 a          464 c          374 g          876 t
ORIGIN      
        1 caagtttatc ctagttatct aggacagccc ctaaactttt tggaagcatc tctaatttat
       61 taatatcgtt catatcccct acacacagaa aaaatcttgg ggaatttcct ttaatctttt
      121 taataaatct tcacaaagcc taaaatgatt taattttagt ttctttttat ttttttgata
      181 aattctagat aacctcgctt attaggaaaa atttcaggat atattccttt ttcttctaaa
      241 gaagtatagt ggattaaatt taaatcagga caaggcgagg aagccgcaga cagtacaaat
      301 agtacggcaa ggcgaggcaa cgccgtacta atccactata tatatccatt catatagaga
      361 aaaattctta tacccaatat aaaacaaggt ctttcattat attttgaaaa acctagaagc
      421 tttgtatcta ttggtttatc aaaattaata attattgcag aagacatgta ggctaataat
      481 cttgatttat ctatatttta ttaattatat atttaacccc gaagtcaata tagaaattta
      541 gtgattttaa agttctctta tcaattttaa tctcagggag tacataaaaa tcctcagaat
      601 aggcaaactc tatatatgca tcatcattcg ataataattc aataatattt ttcatttcat
      661 ccgaaagaaa tgtaaccatc tcatattgac atttattttt agataccatt tttgaatatt
      721 gattaaataa ttttggatta ctatttttag taattaatcc ttccggagta ataaaaccac
      781 tttgtataaa gtcatcactt atattaacag ccttccgaaa aaaaatataa tcacactctt
      841 tgttatcaat ataaaatctc atactcttta ataatgaaat ataagaattt ccagaattac
      901 aagcaatttg aaaaaacatc attactccta aaactcataa atagtaagga atatatatat
      961 atgcatattc cttactattt tttcacaaat tatgcttatc tatgcttatt tccataaaaa
     1021 gcatgccctc gaattttttg acttgagttc tggaaatgag aataatttga atggatttcc
     1081 ttctgtccat gtctaataac attttgtctt ccccaagctt ggtttgctac cgctcttgct
     1141 tgttgagatg ttcgtgcata gacattagct cctcttgata aagcttgtgc tgcaacacgc
     1201 tgcgtaaccc aacgctgcgc aaggaatctg cccgcatgca aaattcctat tgccaccaac
     1261 ggtgccgcag ccccttcagt ttctttcatc tccttttgag aaagctccgc caactgcatc
     1321 ggcgcatctg cccgcgtgtg gaacacttgg tcttcaaatg cctgattgtc caagccgttt
     1381 gccattgcgg gggcgattat agacagcatc atcgtggctt cagtgatttg ttttttcata
     1441 acaaattctt agggaggaga taatgttcca tcaaaatcct cctgaaaggc tgcctgaaat
     1501 ttccaagcag cctttttgat tgcttattaa tcggatagtg cgacaaatat aaagtgggcg
     1561 gcaatcattg ccgtaacggc agccataact gttttatttt cttcaaacca aagcaagttg
     1621 agaaacataa acatcaagaa atatgatggt tcttcagttt tgccgctgct ttcttgagtt
     1681 agaagataaa atatagatat ggcggttatg ccgattgctg cttttattaa aatatatttg
     1741 tttttcatca tcttgtatgg tttttcatgc aagtttcaac aataaaattc ggaataagat
     1801 agagagatgt tgatatatca gataaatata tagtggatta acaaaaacca gtacagcgtt
     1861 gccttgcctt agctcaaaga gaacgattct ctaaggtgct gaagcaccga gtgaatcggt
     1921 tccgtactat ttgtactgtc tgcggcttcg tcgccttgtc ctgatttaaa tttactccac
     1981 tatacaaaac aatcaagaaa ctattggcag tatgtataat cttgaatttc tttttttgat
     2041 gaaattaaaa taatgaaata tatggtaaac gttacaacgg aagccttagc ctcccgattg
     2101 cagtatttca attccaagat aaatgccgca tagaaaagat aaaatactta aatggttttt
     2161 agcggcttaa atatcttatt taattttatt gattgttttc ccaatagttt ccgcaataaa
     2221 taataagaag tagaataata aacaaacaga acatgaaaat aaaaataaat ctttcatcat
     2281 ctcagtaata aaattccaaa gattttcctt atttccatca acaataaaaa ttatcagttc
     2341 atgaataaag aatactgcaa agaaaatgct tcctgccgtt agggatattc ctaacctgct
     2401 tatttttcct tctctatatt tacctgataa atagcctaaa aaagctgcaa tcaaatcatc
     2461 cattttaaaa atttctgtaa aaacaggtaa tgtctttaat tcattacctg ttttgccact
     2521 atatctatga tataagcgtc tcttaccctt cagcctcttt gg
//
LOCUS       DAWFHY010000143         2531 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.143, whole genome shotgun sequence.
ACCESSION   DAWFHY010000143 DAWFHY010000000
VERSION     DAWFHY010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.143"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..2126
                     /locus_tag="WGQ68_002021"
     CDS             <1..2126
                     /locus_tag="WGQ68_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0715749.1"
                     /translation="NKNNPNPNAKALSPALTAPALWFGPVKDGKAEMYSASVSTYPDS
                     SSSQIFLQNLSRKDDTSKPGRYSLKPLSTSEIKSKEPNFTGRQTIIRLDSGVREIKLD
                     RSNEATGLNGNDGKNETFGIVSEGSFMPDASEWKKVLLPWTVRASNDDNQFKTFNQEE
                     KDNKPKYSQKYRSRDSSKRERDLGDIVNSPIVAVGEYLATSANDGMVHIFKKNGTDER
                     GYNLKLSYIPGTMPRKDIESKDSTLAKELRAFAEKGYVGDRYGVDGGFVLREVERDGK
                     TRVFMFGAMGFGGRGAYALDLTKADGSDPTAVSLFDVKNDDKNNNGVKLGYTVGTPQI
                     GKTHDGKYAAFLASGYATKKIDDPTNKTALYVYDLENNNGTPIATINVPDGKGGLSSP
                     TLVDKDLDGTVDIAYAGDRGGNMYRFDLSGQDPNQWSVRTIFSGNKPITSAPAISQLK
                     DKRVVIFGTGSDLSEEDVDNKDIQHVYGIFDDDTATNNVKVDLKGLGGGLLEQHLTQE
                     DKTLFLTDYKRSDGSGSKGWVVKLEAGQRVTVKPTVVLRTAFVTIRKYTTDGCGAETA
                     ILGINTADGGKLTKKSARPIVPEANQAVAQYSGHKQTAKGKSIPIGCMWKNNETVCPN
                     GYVYDKPVNVRYLDEKKTDGFSTTADGDAGGSGIDPAGKRSGKNNRCFSQKGVRTLLM
                     NDLDSLDITGPTCGMKRISWREIFY"
BASE COUNT          709 a          644 c          654 g          524 t
ORIGIN      
        1 tcaacaaaaa taaccccaat cccaacgcta aagccctttc ccccgccctg actgcccccg
       61 cgctgtggtt cggacctgtg aaagatggta aggcggagat gtattccgct tcggtttcca
      121 cctaccccga cagttcgagc agccaaattt tcctgcaaaa cctttcccgc aaggatgaca
      181 caagcaaacc gggccgctat tccctcaaac ccttgagtac gtcggagatt aaaagtaaag
      241 agccgaattt cacggggcgg caaacaatca tccgattgga tagcggcgta cgtgagatca
      301 aactggatag aagcaatgag gccaccggtt taaatggaaa tgacggcaaa aacgaaactt
      361 tcggcattgt tagtgaaggg agcttcatgc ctgatgccag cgagtggaaa aaagtattgc
      421 tgccttggac ggttcgtgct tccaatgatg acaatcaatt taaaacattt aaccaagaag
      481 aaaaagacaa caagccaaaa tacagtcaaa aataccgcag ccgcgacagc agcaagcgcg
      541 agcgtgattt gggcgacatc gtcaacagcc ccatcgtggc ggtcggcgag tatttggcta
      601 cttccgcgaa cgacgggatg gtgcatatct tcaaaaaaaa cggcacggat gaacgcggct
      661 acaatctgaa gctcagctac atccccggaa cgatgccgcg caaggatatt gaaagcaaag
      721 attctacact cgccaaagag ctgcgcgcct ttgccgaaaa aggctatgtg ggcgaccgct
      781 acggcgtgga cggcggcttt gtcttgcgcg aagtcgaacg ggacgggaaa acccgcgtgt
      841 ttatgttcgg cgcgatgggt tttggcggca gaggtgcgta tgccttggat ttgaccaaag
      901 ccgacggcag tgacccgacc gccgtttccc tgtttgatgt caaaaatgac gataaaaaca
      961 ataatggcgt gaaattaggc tacaccgtcg gcacgccgca aatcggcaaa acccacgacg
     1021 gcaaatacgc cgctttcctc gcctccggtt atgcgactaa aaaaattgac gacccaacaa
     1081 ataaaaccgc gctgtatgtg tatgatttgg aaaacaacaa cggtacgccg attgcaacaa
     1141 tcaacgtacc cgacggcaag ggcgggcttt cgtcccccac gctggtggat aaagatttgg
     1201 acggcacggt cgatatcgcc tatgccggcg atcgcggcgg gaatatgtac cgctttgatt
     1261 tgagcggtca agatcctaat caatggtctg tacgcaccat tttcagcggc aacaaaccga
     1321 ttacttccgc gcccgccatt tcccaactga aagacaaacg cgtggttatc ttcggcacgg
     1381 gcagcgattt gagtgaggag gatgtggaca acaaagacat ccaacacgtt tacggtattt
     1441 ttgacgacga tacggcgacg aataatgtaa aggtagacct caaaggtttg ggaggtgggc
     1501 tgctcgagca acaccttact caggaagata aaaccttatt tctgaccgat tataagcgat
     1561 ccgacggatc gggcagcaaa gggtgggtag tgaaattgga agccggacag cgcgttaccg
     1621 tcaaaccgac cgtggtattg cgtaccgcct ttgtaaccat ccgcaaatat accaccgacg
     1681 gctgcggcgc ggaaaccgcc attttgggca tcaataccgc cgacggcggc aagctgacca
     1741 agaaaagcgc gcgcccgatt gtgccggaag ccaatcaggc tgtcgcgcaa tattccggcc
     1801 ataagcaaac cgccaaaggc aaatccatcc ctataggttg tatgtggaaa aacaatgaaa
     1861 ccgtctgccc gaacggatat gtttacgaca aaccggttaa tgtgcgttat ctggatgaaa
     1921 agaaaacaga cggattttca acaacggcag acggcgatgc gggcggcagc ggtatagacc
     1981 ccgccggcaa gcgttccggc aaaaacaacc gctgcttctc ccaaaaaggt gtgcgcaccc
     2041 tgctgatgaa cgatttggac agcttggaca ttaccggccc gacgtgcggt atgaaacgaa
     2101 tcagctggcg tgaaatcttc tactgatttg cacgcgaaaa tgccgtccga aaggttttcg
     2161 gacggcattt tttgcgtttt tcgggagggg cgggttcgta aaaggcgggc tatagggtag
     2221 gcttcatctc gccaatctca ctgaatccat caatttccac aattcaatta aataccgtca
     2281 aaccgatgcc gtcattcccg cgcaggcggg aatctagacc ttagaacaac agtaatattc
     2341 aaaggttagc tgaagcttta gagattctgg attcccactt tcgtgggaat gacgggattt
     2401 gagattgcga catttatcgg gagcaacaga aaccgctctg ccgtcattcc cgcgcaggcg
     2461 ggaatctaga ccttagaaca acagcaatat tcaaagatta tctgaaagtc cgggattctg
     2521 gattcccact t
//
LOCUS       DAWFHY010000144         2451 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.144, whole genome shotgun sequence.
ACCESSION   DAWFHY010000144 DAWFHY010000000
VERSION     DAWFHY010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2451)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2451)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2451
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.144"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..1032
                     /gene="rfbB"
                     /locus_tag="WGQ68_002022"
     CDS             <1..1032
                     /gene="rfbB"
                     /locus_tag="WGQ68_002022"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEZ0715750.1"
                     /translation="IGSAVVRHIIRNTQDSVVNLDKLTYAGNLESLTDIADNPRYAFE
                     QVDICDRAELDRVFAQHRPDAVMHLAAESHVDRSIGSAGEFIQTNIVGTFNLLEAARA
                     YWQQMPSEQHEAFRFHHISTDEVYGDLGGTDDLFTETAPYAPSSPYSASKASSDHLVR
                     AWLRTYGLPTIVTNCSNNYGPYHFPEKLIPLMILNALDGKPLPVYGDGMQIRDWLFVE
                     DHARALYQVVTEGVVGETYNIGGHNEKANIEVVRTICALLEELVPNKPQGVARYEDLI
                     TYVKDRPGHDVRYAIDAAKIGRELGWQPQETFESGIRKTVEWYLDNKTWWQNVLNGSY
                     RLERLGTGK"
     gene            1095..1961
                     /gene="rfbA"
                     /locus_tag="WGQ68_002023"
     CDS             1095..1961
                     /gene="rfbA"
                     /locus_tag="WGQ68_002023"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEZ0715751.1"
                     /translation="MKGIILAGGSGTRLYPITRGVSKQLLPVYDKPMIYYPLSVLMLA
                     GIRDILVITAPEDNASFKRLLGDGSDFGISISYAVQPSPDGLAQAFIIGEEFIGNDNV
                     CLVLGDNIFYGQSFTQTLKQAAAQTHGATVFAYQVKNPERFGVVEFNENFRAVSIEEK
                     PQRPKSDWAVTGLYFYDNRAVEFAKQLKPSARGELEISDLNQMYLEDGSLSVQILGRG
                     FAWLDTGTHESLHEAASFVQTVQNIQNLHIACLEEIAWRNGWLSDEKLEELARPMAKN
                     QYGQYLLRLLKK"
     gene            2015..>2451
                     /gene="rfbC"
                     /locus_tag="WGQ68_002024"
     CDS             2015..>2451
                     /gene="rfbC"
                     /locus_tag="WGQ68_002024"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016670590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEZ0715752.1"
                     /translation="MNIIDTAIPDVKLLEPQVFGDARGFFMETFRDEWFKTQVCERTF
                     VQENHSKSGKGVLRGLHYQTENTQGKLVRVVVGEVFDVAVDMREGSPTFGKWAGATLS
                     AQNQCQLWIPEGFAHGFCVLGDAAEVVYKCTDYYKPEAEQVLI"
BASE COUNT          622 a          682 c          615 g          532 t
ORIGIN      
        1 atcggttctg ccgttgtccg tcatattatc cgaaacaccc aagattccgt cgtcaacctc
       61 gacaaactga cctacgcggg caatctcgaa tcgctgaccg acattgccga taatccccgc
      121 tatgctttcg agcaagtgga catttgcgac cgcgccgaac tcgaccgcgt attcgcacaa
      181 caccggcccg atgccgtgat gcacttggcg gcggaaagcc atgtcgaccg ctctatcggt
      241 tcggcaggcg agtttatcca aaccaatatc gtcggcacat tcaatctgct tgaagcagcc
      301 cgcgcctact ggcaacaaat gccgtctgaa cagcacgaag ccttccgttt ccaccatatt
      361 tccaccgatg aagtctatgg cgatttaggc ggcacggacg atttgtttac cgaaaccgcg
      421 ccctacgcgc cgtccagccc ctactctgcc tctaaagcgt ccagcgacca cctcgtccgc
      481 gcgtggttgc gtacttacgg cttgccgacc attgtaacca actgctccaa caactacggt
      541 ccttaccatt ttccggaaaa actcattcct ttgatgattc tgaacgcgct tgacggcaaa
      601 ccgctgcctg tgtacggcga cggtatgcaa atccgcgact ggctgtttgt cgaagaccac
      661 gcgcgcgcac tgtatcaggt tgttaccgaa ggtgttgtcg gcgaaaccta caatatcggc
      721 ggccacaatg aaaaagccaa tattgaagtg gtgcgcacca tttgcgccct gctggaagaa
      781 ttggtgccga acaaaccgca aggcgtggcg cgttatgaag atttgattac ttatgtgaaa
      841 gaccgccccg gccacgatgt gcgctatgcg attgatgcag caaaaatcgg ccgcgagttg
      901 ggttggcagc cgcaggaaac ttttgaaagc ggcattcgca aaacagtgga atggtatctg
      961 gacaacaaaa cctggtggca aaatgtattg aacggcagct atcgtttgga acgcttaggt
     1021 acaggcaaat agttttcaga cggcatcccg acgcaatgcc gtctgaaaac ccatcgcaaa
     1081 ggaagaaaga aaagatgaaa ggcatcatac tggcaggcgg cagcggcacg cgcctctacc
     1141 ccatcacgcg cggcgtatcc aaacagctgc tgcccgtgta cgacaaaccg atgatttatt
     1201 accccttgtc ggttttgatg ctggcgggaa tccgcgatat tttggtgatt accgcgcctg
     1261 aagacaacgc ctctttcaaa cgcctgcttg gcgacggcag cgatttcggc atttccatca
     1321 gttatgccgt gcaacccagt ccggacggct tggcacaggc atttatcatc ggcgaagaat
     1381 ttatcggcaa cgacaatgtt tgcttggttt tgggcgacaa tattttttac ggtcagtcgt
     1441 ttacgcaaac attgaaacag gcggcagcgc aaacgcacgg cgcaaccgtg tttgcttatc
     1501 aggtcaaaaa ccccgaacgt ttcggcgtgg ttgaatttaa cgaaaacttc cgcgccgttt
     1561 ccatcgaaga aaaaccgcaa cggcccaaat ccgattgggc ggtaaccggc ttgtatttct
     1621 acgacaaccg cgccgtcgag ttcgccaaac agctcaaacc gtccgcacgc ggcgaattgg
     1681 aaatttccga cctcaaccaa atgtatttgg aagacggttc attgtccgtc caaatattag
     1741 gacgcggttt cgcatggttg gacaccggca cccacgagag cctgcacgaa gccgcttcat
     1801 tcgtccaaac cgtgcaaaac atccaaaacc tgcacatcgc ctgcctcgaa gaaatcgcct
     1861 ggcgcaacgg ttggctttcc gatgaaaaac tggaagaatt ggcgcgcccg atggcgaaaa
     1921 accaatacgg ccaatatttg ctgcgcctgt tgaaaaaata atgtttgagg ccgtctgaaa
     1981 cttttcagac ggcctttaga tgaaagataa aaagatgaac atcattgata ccgccattcc
     2041 tgacgtaaaa ctgcttgagc cccaagtctt cggcgacgcg cgcggctttt ttatggaaac
     2101 cttccgcgac gagtggttta aaacccaagt ctgcgaacgc accttcgtgc aggaaaacca
     2161 ctccaaatcc ggcaaaggcg tattgcgcgg cctgcactac caaaccgaaa acacacaagg
     2221 caaactcgta cgcgtagttg tcggcgaagt gttcgacgtg gccgtcgata tgcgcgaagg
     2281 ttcgccgact ttcggcaaat gggcgggcgc aaccctgtcc gcgcaaaacc aatgccagct
     2341 ttggataccc gaaggcttcg cgcacgggtt ttgcgttttg ggcgacgcag cggaagtcgt
     2401 gtacaaatgc acggactatt acaagcctga agccgaacag gttttaatat g
//
LOCUS       DAWFHY010000145         2446 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.145, whole genome shotgun sequence.
ACCESSION   DAWFHY010000145 DAWFHY010000000
VERSION     DAWFHY010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.145"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(425..2305)
                     /locus_tag="WGQ68_002025"
     CDS             complement(425..2305)
                     /locus_tag="WGQ68_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacB/RNase II family 3'-5' exoribonuclease"
                     /protein_id="HEZ0715753.1"
                     /translation="MNIFYEESGQFKIAAIIQKNDATYQVDTPHGKRTKVKANNVFAE
                     FDGDMAAFLENAQAQAADIDTDLLWEVCGEEEFTAEAIAEEYYGHAPTKTELAATLIA
                     LYAAPMYFYKKAKGVFKAAPEETLKQALAAIERKKQQDAQIDAWAEALKRGEMPSEIA
                     ADLKTILHAPDKQSLTYKAFTKAADALKTSAYELAKKTGGITSIPQYLQDGFEIKYFP
                     KGTGFPDLPLPEMPDLPKADVTAFSIDDESTTEVDDALSLTDLDNGTKRVGIHIAAPS
                     LAIKQGDKMEKNIMERLSTVYFPGGKITMLPENWIAAFSLDAGAYRPSISIYFDVDSE
                     FNVGAPTCKIEAVNIAENLRIQAIEPHFNAEAGLDEAGEMMFAHHQDLIWFYQFATAL
                     QKARGKYEPDRAPQYDYSIELDEEGNVSVVRRERGSPIDTLVSEMMILANSTWAQMLD
                     ENELPGLFRVQPAGKVRMSTKSEPHIGMGVQHYGWFTSPLRRAADYINQKQLISLIDD
                     SAEPLYQNSDAELFAALRDFDAAYTAYADFQRQMEAYWSLVYLQQQGTSELTATILKE
                     DLVRIEGLPLVTRATGIPFDALPKSQALFKITELDAEKQFVSLNYIKAVAPAGTTAGN
                     AV"
BASE COUNT          490 a          599 c          730 g          627 t
ORIGIN      
        1 atttccacct gcgcgggaat gacgattcag aagttgcccg aaacctaaaa aactgaaacc
       61 gaacgagccg gatttccgct ttcgcgggaa tgacggcgta taagttcccg tgcggacaga
      121 cctatattcc cacctgcgcg ggaatgacga ttcagaagtt acacgaaact caaaaaaaac
      181 gaaaccgaac gaaccggatt cccgctttta cgggaatgac ggcgcataag ttcccgtgcg
      241 gacagaccta gattcccacc tgcgtgggaa tgacgggatt ttagattgcg ggtatttatc
      301 gggaacggcg gcttggaagt tcattgaaac ggaaaaacaa cggaaaccca aaaaaccgga
      361 ttcccgactg tgggaatgat gagattcagg tttctgtttt tgccggagtt tgccgtatcg
      421 ggcttcagac ggcattgcct gccgttgtac ccgcgggtgc gactgccttg atgtagttga
      481 gcgagacaaa ctgcttctcg gcatccaatt cggtgatttt gaacaatgcc tgtgatttgg
      541 gcagcgcgtc aaacggaata ccggtcgcgc gcgttaccag cggcaggcct tcaatgcgga
      601 cgaggtcttc tttgaggatg gtggccgtca gctcgctcgt gccttgctgt tgcagataga
      661 cgaggctcca gtaggcttcc atttggcgtt ggaagtcggc gtaggcggta taggcggcat
      721 caaagtcgcg cagcgcggcg aaaagctcgg catcgctgtt ttgatacagc ggctcggcag
      781 agtcgtctat caggctgatc agctgctttt ggttgatgta gtcggcggcg cggcgcagcg
      841 gcgaagtgaa ccagccgtaa tgctgcacgc ccatgccgat gtgcggctcg gatttggtgc
      901 tcatgcgcac tttgcctgcc ggttggacgc ggaaaaggcc gggcagctcg ttttcgtcca
      961 gcatttgcgc ccaagtgctg ttggcaagaa tcatcatctc gctgaccagc gtatcgatgg
     1021 gcgaaccgcg ttcgcggcgg acgacggata cgttgccttc ctcatccaat tcgatgctgt
     1081 aatcgtattg cggcgcacgg tcgggctcgt atttgccgcg cgctttttgc agggcggtgg
     1141 cgaattgata gaaccaaatc aggtcttgat ggtgggcgaa catcatttcg cccgcttcgt
     1201 ccaagcccgc ttcggcgttg aaatgcggtt cgatggcttg gatacgcagg ttttcggcga
     1261 tgttgaccgc ttcgattttg caggtcggcg cgccgacgtt gaactcgctg tccacatcga
     1321 aataaatgct gatggaaggg cggtatgcac ctgcatcaag gctgaacgcg gcaatccagt
     1381 tttcgggcag catcgtgatt ttgccgccgg ggaaataaac cgtgctcaag cgttccatga
     1441 tgtttttttc cattttgtcg ccttgtttaa tggcaagcga cggcgcggcg atgtggatgc
     1501 cgacgcgctt cgtgccgttg tccaagtcgg tcaggcttaa agcgtcgtcc acttcggtgg
     1561 tcgattcgtc gtcaatggaa aaggcggtaa cgtcagcctt gggcaggtcg ggcatttcgg
     1621 gaagcggaag gtcggggaag cctgttcctt tggggaagta tttgatttca aacccgtctt
     1681 gcaggtattg gggaatggac gtaatgccgc ccgttttttt cgccaattcg taggcagagg
     1741 ttttcagcgc gtcggcggct ttggtaaagg ctttgtaggt cagcgactgc ttgtcgggcg
     1801 cgtgcaggat ggttttcaga tccgccgcga tttcagacgg catctcgccg cgtttcaagg
     1861 cttccgccca agcgtcgatt tgcgcgtctt gctgtttttt gcgttcgatg gcggcaagtg
     1921 cctgtttcaa agtttcttcg ggcgcggctt tgaacacgcc tttggctttt ttgtagaaat
     1981 acatcggcgc ggcgtacagc gcaatcaagg tcgccgccag ctcggttttg gtcggcgcat
     2041 ggccgtaata ttcttcggct atggcttcag cggtaaattc ctcttcgccg catacttccc
     2101 acaataaatc ggtgtcgatg tccgccgcct gtgcctgcgc gttttccaaa aacgccgcca
     2161 tatcgccgtc aaactcggca aagacgttgt tcgccttcac tttggtgcgt ttgccgtgtg
     2221 gggtatcgac ttggtaggtg gcatcgtttt tttggatgat ggcggcgatt ttgaattggc
     2281 cggactcttc gtaaaaaata ttcatttttc ggatttttct gtggaaactc aagcgggcga
     2341 ttttagcaga ttaccgaaaa tgccgtctga aaaaaggttg ggagagggtt ggcgcgtctt
     2401 tgcggtgctt gcgttatagt ggattaacaa aaaccagtac agcgtt
//
LOCUS       DAWFHY010000146         2432 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.146, whole genome shotgun sequence.
ACCESSION   DAWFHY010000146 DAWFHY010000000
VERSION     DAWFHY010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.146"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            35..1390
                     /locus_tag="WGQ68_002026"
     CDS             35..1390
                     /locus_tag="WGQ68_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HEZ0715754.1"
                     /translation="MSEYTPQTAKQGLPALAKSTIWMLSFGFLGVQTAFTLQSSQMSR
                     IFQTLGADPHNLGWFFILPPLAGMLVQPIVGHYSDRTWKPRLGGRRLPYLFYGTLIAV
                     IVMILMPNSGSFGFGYASLAALSFGALMIALLDVSSNMAMQPFKMMVGDMVNEEQKSY
                     AYGIQSFLANTGAVVAAILPFVFAYIGLANTAEKGVVPQTVVVAFYVGAALLIITSAF
                     TIFKVKEYDPETYARYHGIDVAANQEKANWFELLKTAPKAFWTVTLVQFFCWFAFQYM
                     WTYSAGAIAENVWHTTDASSVGYQEAGNWYGVLAAVQSVAAVICSFVLAKVPNKYHKA
                     GYFGCLALGALGFFSVFFIGNQYALVLSYTLIGIAWAGIITYPLTIVTNALSGKHMGT
                     YLGLFNGSICMPQIVASLLSFVLFPMMGGLQANMFLVGGVVLLLGAFSVFLIKETHGG
                     V"
     gene            1394..2404
                     /locus_tag="WGQ68_002027"
     CDS             1394..2404
                     /locus_tag="WGQ68_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015815867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEZ0715755.1"
                     /translation="MSDTPATRDFGLIDGRAVTGYVLSNRRGTRVCVLDLGGIVQEFS
                     VLADGVRENLVVSFDDAASYADNPFQINKQIGRVAGRIRGAAFDINGRTYRVEANEGR
                     NALHGGSHGLAVTRFNAVAADGRSVVLRSRLQQSADGYPNDLDLDISYRLDEDDRLTV
                     TYRATALGDTVFDPTLHIYWRLDAGLHDAVLHIPQGGHIPADAEKLPVSTVSDGLEVF
                     DFSRPKPLDAAVAALRRETGRAGFDDAYRVPSDIGRPAAVLQAGRRCRISIYSDRNGL
                     VIFTAAPQDFARHDAGVYDALATEAQTLPDSLHWPEFGNIRLNKGDTREATIAYGIES
                     LS"
BASE COUNT          455 a          656 c          726 g          595 t
ORIGIN      
        1 actatacttt tcaaatcaaa aaaggattta ccttatgtcg gaatatacgc ctcaaacagc
       61 aaaacaaggt ttgcccgcgc tggcaaaaag cacgatttgg atgctcagtt tcggctttct
      121 cggcgttcag acggccttta ccctgcaaag ctcgcaaatg agccgcattt ttcaaacgct
      181 aggcgcagac ccgcacaatt tgggctggtt tttcatcctg ccgccgctgg cggggatgct
      241 ggtgcagccg attgtcggcc attactccga ccgcacttgg aagccgcgtt taggcggccg
      301 ccgtctgccg tatctgtttt atggcacgct gattgcggtt attgtgatga ttttgatgcc
      361 gaactcgggc agcttcggtt tcggctatgc gtcgctggcg gctttgtcgt tcggcgcgct
      421 gatgattgcg ctgttggacg tgtcgtcgaa tatggcgatg cagccgttta agatgatggt
      481 cggcgatatg gtcaacgagg agcagaaaag ctacgcctac gggattcaaa gtttcttagc
      541 gaatacgggc gcggtcgtgg cggcgattct gccgtttgtg ttcgcgtata tcggtttggc
      601 gaacaccgcc gagaaaggcg ttgtgccaca aaccgtggtc gtagcattct atgtgggcgc
      661 ggcattactg attattacca gtgcgttcac aatcttcaaa gtcaaagaat acgacccgga
      721 aacctatgcc cgttaccacg gcatcgatgt cgccgcgaat caggaaaaag ccaactggtt
      781 cgaactctta aaaacggcac ctaaagcgtt ttggacggtt actctggtac agtttttctg
      841 ctggttcgcc ttccaatata tgtggactta ctcggcaggc gcgattgcgg aaaacgtctg
      901 gcacaccact gatgcgtctt ccgtaggtta tcaggaggcg ggcaactggt acggcgtttt
      961 ggcggcggtg cagtcggttg cggcggtgat ttgttcgttt gtattggcga aagtgccgaa
     1021 taaataccat aaggcgggtt atttcggctg tttggctttg ggcgcgctcg gctttttctc
     1081 cgttttcttc atcggcaacc aatacgcgct ggtgttgtct tataccttaa tcggcatcgc
     1141 ttgggcgggc attatcactt atccgctgac gattgtaacc aacgccttgt cgggcaagca
     1201 tatgggcact tacttgggcc tgtttaacgg ctctatctgt atgccgcaaa tcgtcgcttc
     1261 gctgttgagt ttcgtgcttt tcccgatgat gggcggtttg caggccaata tgttcttggt
     1321 agggggcgtc gtcctgctgc tgggcgcgtt ttccgtgttc ctgattaaag aaacacacgg
     1381 cggggtttga gcgatgagcg atacccccgc tacccgcgat ttcggtctga tcgacgggcg
     1441 tgccgtaacc ggctatgtgc tgtccaaccg gcgtggtacg cgtgtctgcg tgctggactt
     1501 gggcgggatt gtgcaggaat tttccgtttt ggcagacggc gtgcgcgaaa acctcgtggt
     1561 gtcgttcgat gatgcggctt cctatgcgga caatccgttt cagattaaca aacagatagg
     1621 gcgcgtggcc ggacgcatcc gcggtgcggc gttcgacatc aacggcagga cttaccgcgt
     1681 ggaggccaac gaaggcagga acgcgctgca cggcggttcg cacgggctgg ccgttacccg
     1741 tttcaacgcg gtggcggcag acggccgttc ggtggtgctg cgcagccgcc tgcaacagtc
     1801 ggccgacggt tatcccaacg atttggattt ggatatttcc taccgcttgg acgaggacga
     1861 ccggcttacc gttacctatc gcgccaccgc gctcggcgac acggtgttcg acccgacgct
     1921 gcacatttac tggcggctgg acgcgggcct gcacgatgcg gttctgcata ttccgcaggg
     1981 cggacatatt ccggccgatg ccgaaaaact gcccgtctca acggtttcag acggcctcga
     2041 agtatttgat ttcagccggc ccaagccgct ggatgccgcc gttgccgccc tgcgccgcga
     2101 aacgggtcgg gccggttttg acgacgctta ccgcgtgccg tccgatatag gccgtcccgc
     2161 cgctgtgttg caagccggac gccgctgtcg tatcagcata tacagcgacc gcaacggctt
     2221 ggtcatcttt accgccgccc cgcaggattt cgcgcggcac gatgcgggcg tttacgacgc
     2281 gctggcgacc gaggcgcaga cgctgcccga cagcctgcat tggcccgagt tcggcaatat
     2341 tcgtctgaac aagggtgata ccagggaggc gacgattgct tacggcatcg aatccctttc
     2401 ttaggagctt cctaacaccg gttgcagacg gc
//
LOCUS       DAWFHY010000147         2305 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.147, whole genome shotgun sequence.
ACCESSION   DAWFHY010000147 DAWFHY010000000
VERSION     DAWFHY010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.147"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(50..1459)
                     /gene="dacB"
                     /locus_tag="WGQ68_002028"
     CDS             complement(50..1459)
                     /gene="dacB"
                     /locus_tag="WGQ68_002028"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEZ0715756.1"
                     /translation="MNFPKTAASLLLLLASLAAHALDTGRIPQNEIAVYVQELDSGKV
                     IIDHRSDVPVNPASTMKLVTAFAAFKTFGSNYRWATEFKSNGTVNDGTLDGNLYWAGS
                     GDPVFNQENLLAVQRQLREQGIRNITGHLMLDHSLWGEVGSPDDFEADSGSPFMTPPN
                     PTMLSAGMVMVRAERNAADSTDILTDPPLPHIFAQNNLKITASQAACPSIKKLMRASF
                     SDNTLKLRGNIPESCLGKPVGVRMFALDELIRQSFTNHWLLGGGRISDGIGISDTPEG
                     AQTLAVAHSKPMKEILTDMNKRSDNLIARSVFLKLGGNGKLPAVSEQAASAVRRELAV
                     SGIDVADLVLENGSGLSRKERVTARMMAQMLETAYFSPFAQDFIDTLPIAGTDGTLRN
                     RFKQSGGLLRLKTGTLNNVRALAGYWLGDKPMAVVVIINSGRAVSLLPDLDNFVANNI
                     ISGGDGWLDAKLMCKERRA"
     gene            1624..2190
                     /locus_tag="WGQ68_002029"
     CDS             1624..2190
                     /locus_tag="WGQ68_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEZ0715757.1"
                     /translation="MAKKISILVGSLRRASFARKVALNAAEMFPEGWQAEIVEIGHLP
                     LYNFDYDDPAVEDVPLPESYTVFRETIKASDGILFVTSENNRTIPACLKNAVDIGSKP
                     NADVAWKNKPAGIISHSVGKMGGYSSQKNLRLALSYFDMPVTGQPEVFLGNSPTLFDE
                     NGKLIDSARDFVQSYINQFVGLIERNAK"
BASE COUNT          528 a          562 c          663 g          552 t
ORIGIN      
        1 tagttatcta ggacagcccc ttaaatttaa tccactatat tttcctgttt caggctcggc
       61 gttctttgca catcagtttc gcatccagcc agccatcgcc gccggagatg atgttgttgg
      121 caacgaagtt gtccaagtcg ggcagcaggg aaacggcgcg gccgctgttg atgatgacga
      181 ccaccgccat cggtttgtcg cccagccaat aacctgcaag ggcgcggaca ttgttgagcg
      241 tgccggtttt taagcgcaac agcccgccgc tttgtttgaa gcggttgcgt aaagtcccgt
      301 ctgtgccggc gatgggcagc gtatcgatga aatcttgtgc aaacgggctg aaataagccg
      361 tttccaacat ttgcgccatc attctcgccg ttaccctttc ttttctagac agacctgaac
      421 cgttttccaa aaccaaatcc gcaacatcga tgcccgacac ggcaagttca cgccggacgg
      481 cagacgctgc ctgttcggaa acggcgggca gtttgccgtt gccgccgagt ttgaggaaga
      541 cggaacgcgc aattagattg tccgaacgct tgttcatgtc cgtcaaaatt tccttcatcg
      601 gctttgagtg tgcaacggca agcgtctgcg cgccttccgg cgtgtcggat atgccgatgc
      661 cgtctgaaat ccgtccgccg ccgagcagcc agtggttggt aaaactttgc cggatcagtt
      721 cgtcaagcgc gaacatccgg acaccgacag gcttgcccaa acagctctcg ggaatattgc
      781 cgcgcaattt cagcgtattg tccgaaaaag atgcacgcat cagttttttg atcgaagggc
      841 aggcagcttg ggaggcggta attttcaagt tgttttgggc gaaaatatgc ggcaaaggcg
      901 gatcggtgag gatgtcggta ctgtcggcgg cattgcgttc ggcgcgcacc ataaccatac
      961 cggcagacag catagttgga ttggggggcg tcataaacgg cgaaccgctg tcggcttcga
     1021 aatcgtcggg gctgccgact tcgccccaca ggctgtggtc gagcatcagg tgtcccgtga
     1081 tattgcgtat gccttgttcg cgcaactggc gttggacggc aagcaggttt tcctgattga
     1141 aaacggggtc gccgctgccc gcccaataca ggtttccgtc aagcgtgccg tcgtttaccg
     1201 taccgttgct tttaaactcg gtcgcccagc ggtaattgct gccgaaggtt ttgaaggcgg
     1261 caaacgcggt aacgagtttc attgtggagg cggggttgac ggggacatcc gagcggtggt
     1321 caatgatgac ttttccgctg tcaagctctt ggacatatac ggcgatttcg ttttgcggaa
     1381 tgcgacccgt atcgagcgcg tgtgcggcga gggaggcgag aagcagcagc agggaggccg
     1441 ctgttttggg gaaattcata ggtaaatctg ttacataaaa aaagcaatta taaggcaaag
     1501 ccggataagt gggaacgctg tggcggcggg ccggcttgtt tgcaggggaa atcacatata
     1561 taataatcgc tatcattatg aaatgattga gacgcacaaa cttaatattc aggaggaatg
     1621 attatggcta agaaaatcag tattttggtg ggcagcctgc gccgtgcttc ttttgcgcgc
     1681 aaagtggcat tgaatgcggc ggagatgttc cccgaaggct ggcaggcgga aatcgtcgaa
     1741 atcggacatc tgccgcttta caatttcgat tatgacgacc ctgcggtgga agatgtgccg
     1801 ctgcccgaaa gctacacggt tttccgcgaa acgattaagg cttcggacgg cattttgttc
     1861 gttacgtccg aaaataaccg caccattccc gcctgtttga aaaatgcggt ggacatcggc
     1921 tcgaaaccga atgccgacgt ggcgtggaaa aacaaaccgg ccggcatcat cagccattcc
     1981 gtcggcaaga tgggcggtta cagttcgcaa aaaaacctgc gccttgcgct gtcttatttc
     2041 gatatgcccg taaccggaca gccggaagtg tttttgggca attcgccgac gctgtttgat
     2101 gaaaacggca aattgatcga ctcggcaagg gattttgttc agtcatatat taatcagttt
     2161 gtcggtttga ttgaaagaaa tgccaaataa acaaacaagt cgaaaggccg caaaaccggt
     2221 tcgggttttg cggctttttt atatgggctg ggcagcgtca aggtttgccg tttgccggat
     2281 aatgcgcggc aaatgaccgg cgtgt
//
LOCUS       DAWFHY010000148         2268 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.148, whole genome shotgun sequence.
ACCESSION   DAWFHY010000148 DAWFHY010000000
VERSION     DAWFHY010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.148"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..574)
                     /locus_tag="WGQ68_002030"
     CDS             complement(<1..574)
                     /locus_tag="WGQ68_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010931119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEZ0715758.1"
                     /translation="MEHKLPQLPYELDALSPHLSKETLEFHYGKHHQTYITNLNNQIK
                     GTEFENLPLEEIVKKSSGGVFNNAAQTWNHTFYWLGFTPKGQGKPSGELAAAIDAKWG
                     SFEKFQEAFSACAAGTFGSGWAWLVKTPAGGLDLVSTSNAATPLTTENTPLLTCDVWE
                     HAYYIDYRNSRPNYLKGFWEIVNWDEVAKRF"
     gene            737..2143
                     /gene="dnaB"
                     /locus_tag="WGQ68_002031"
     CDS             737..2143
                     /gene="dnaB"
                     /locus_tag="WGQ68_002031"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEZ0715759.1"
                     /translation="MNDYTAMPSEDREVGALSLPPHSMEAEQSVLGGLMLENPAWDRI
                     ADVVSGEDFYRHEHRLIFRSIAKLINESRPADVITVQEDLQRNEELEAAGGFEYLITL
                     AQNTPSAANIRRYAEIVRERSIMRQLAEVGTEIARSAYNPQGRDAGQLLDEAENKVFQ
                     IAESTAKSKQGFLEMPDLLKEVVQRIDMLYSRDNPDEVTGVPTGFIDLDKKTSGLQPG
                     DLIIVAGRPSMGKTAFSINIAEHVAVEGKLPVAVFSMEMGGAQLVMRMLGSVGRLDQS
                     VLKTGRLEDEHWGRLNEAVVKLSDAPVYIDETPGLTALELRARARRLARQFNNKLGLI
                     VIDYLQLMAGSGRSDNRASELGEISRSLKALAKELQVPIIALSQLSRTVESRTDKRPM
                     MSDLRESGAIEQDADLIMFMYRDEYYNQDSPMKGLAECIIGKHRNGPVGKIFLTWTGQ
                     FTKFDNAAYIPEEAKIED"
BASE COUNT          549 a          581 c          612 g          526 t
ORIGIN      
        1 cgaagcgttt ggcgacttcg tcccagttga cgatttccca aaaacctttc aggtagttgg
       61 ggcggctgtt gcggtagtcg atgtaatagg cgtgttccca cacgtcgcag gtcagcagcg
      121 gcgtgttttc agtggtcagc ggcgtagcgg cgttggaagt ggaaaccaaa tccaatccgc
      181 cggcaggggt ttttaccagc cacgcccaac cggagccgaa agtaccggca gcgcaagcgg
      241 agaatgcttc ttggaatttc tcgaagctgc cccatttcgc gtcgatggcg gcggccagtt
      301 cgccggaagg tttgccttga cctttgggcg tgaaacccag ccagtagaaa gtgtggttcc
      361 aagtttgggc tgcgttgttg aacacgccgc ccgaagattt tttcacaatc tcttccaaag
      421 gcaggttttc aaattcggtg cctttgattt gattgttcag gttggtgatg taggtttgat
      481 ggtgtttgcc gtagtggaat tccaaagttt ctttgctcag atgcggggac aatgcgtcca
      541 gttcataagg cagttgcggc agcttatgtt ccattttgtg ctcctaatat tgttttaaat
      601 gttgtatttt ggcagtgttg ctgcaaatag ctcagcagcg cacatatttt acctgttttg
      661 agggatggaa accaattaaa cctgctttac gctataatag aaggttgcaa tttcggcacg
      721 acagatagga tgtaccatga acgattacac agccatgccg tctgaagacc gtgaggtcgg
      781 cgcattgtcg ctgcctccac attcgatgga ggcggaacaa tccgttttgg gtgggttgat
      841 gctggaaaat ccggcttggg acaggattgc cgatgtggtt tccggtgaag acttctaccg
      901 ccacgaacac cgcctgattt tccgatccat tgctaaatta atcaatgaga gccgtcccgc
      961 agatgtgatt acggttcagg aagatttgca gcggaacgaa gaattggaag cggcaggcgg
     1021 atttgaatat ctgattacgc tggcgcaaaa taccccgtct gccgccaata tccgccgcta
     1081 cgccgaaatc gtgcgcgagc gttccattat gcgccaactc gccgaagtgg ggacggaaat
     1141 cgcccgcagc gcatacaatc cgcaaggcag ggacgcgggg cagcttttgg acgaggcgga
     1201 aaacaaagta ttccaaatcg ccgaaagcac cgccaaatcc aagcagggct ttttggagat
     1261 gcccgatttg ctgaaagaag tcgtacagcg catcgatatg ctctactcgc gcgacaatcc
     1321 cgatgaagtt accggcgtgc cgacggggtt catcgacctc gacaaaaaaa cctcgggtct
     1381 gcagcccggc gacctgatta tcgttgccgg ccgtccgtct atgggtaaga ccgccttttc
     1441 catcaatatc gccgaacacg ttgccgtaga aggcaagctg ccggttgccg tcttctcaat
     1501 ggagatgggc ggggcgcaac tggtcatgcg tatgctcggc tcggtcggac gcttggatca
     1561 aagcgttttg aaaaccggca ggctcgaaga cgaacactgg ggtcgcctga acgaagcagt
     1621 cgtcaaactc tccgacgcgc ccgtgtacat cgacgagacc ccgggtctga ccgcgctcga
     1681 actgcgcgcc cgtgcccgcc gtctcgcccg tcaatttaac aataagctgg gattaatcgt
     1741 catcgactac ctgcaactga tggcaggatc cggccgttcc gacaaccgag cttcagagct
     1801 gggagagatt tcacgttcgc tcaaagcgtt ggcgaaagaa ttgcaagtcc ccatcatcgc
     1861 cctgtcgcaa ttgagccgta ccgtcgaatc gcgtaccgac aaacgcccga tgatgtccga
     1921 ccttcgcgag tccggcgcaa tcgagcagga tgccgacctg attatgttca tgtaccgcga
     1981 cgaatactac aaccaggact cacccatgaa aggccttgcc gaatgtatca tcggcaaaca
     2041 ccgcaacggt cccgtcggta aaatcttcct cacatggacg ggacaattca ccaaattcga
     2101 caatgctgcc tatattcccg aggaggcaaa gatagaggat taaatggcta tataaaaatt
     2161 tattaggcga aatcaggcaa aatcgtttaa aatcatgctg agagattgcc ctaaaaaata
     2221 aaacgcggtc ttgaggcatt tttgcattca gcccgcatat aattgaaa
//
LOCUS       DAWFHY010000149         2199 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.149, whole genome shotgun sequence.
ACCESSION   DAWFHY010000149 DAWFHY010000000
VERSION     DAWFHY010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.149"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            33..518
                     /locus_tag="WGQ68_002032"
     CDS             33..518
                     /locus_tag="WGQ68_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003713215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715760.1"
                     /translation="MFVNEKYPYAALFAGLVFLTLPFALSVHDALAFAFGRAGLLVSV
                     SDGGFGWRGGWDGTVWFVFGVFAFLNVVVSAGLTKLAYKKMMRRHSRYALFLSGVAAC
                     AAAAVAWIFELLLGSEALGGLRGEAVSEYAFAVWLVAMLTLPKRLTRAPVQPVVFHRK
                     K"
     gene            614..1237
                     /gene="rsmG"
                     /locus_tag="WGQ68_002033"
     CDS             614..1237
                     /gene="rsmG"
                     /locus_tag="WGQ68_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003707255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEZ0715761.1"
                     /translation="MERKERLRAGIAAMGLDILETAQDRLLAYVDLLKKWNKTYNLTA
                     LRDEEKMIVHHLLDSLTLLPYIEGAQTMLDVGSGGGQPGIPAAVCRPDVQITLLDANT
                     KKTAFLQQAVIELGLDNVRVVSGRVEAVSDVRADVITSRAFAELADFVSWTGHLLKDG
                     GYWAAMKGVYPQGEIDRLPQDVCVEKVQRLDVPGLDAERHIAILRKR"
     gene            1336..2109
                     /locus_tag="WGQ68_002034"
     CDS             1336..2109
                     /locus_tag="WGQ68_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEZ0715762.1"
                     /translation="MSANILAIANQKGGVGKTTTTVNLAASLASRGKRVLVVDLDPQG
                     NATTGSGIDKASLQSGVYQVVLGDADVKSATVRSKEGGYAVLGANRALAGAEIELVQE
                     IAREVRLKNALKAVAEDYDFILIDCPPSLTLLTLNGLVAAGGVIVPMLCEYYALEGIS
                     DLIATVRKIRQAVNPDLDITGIVRTMYDSRSRLVAEVSEQLRSHFGDLLFETVIPRNI
                     RLAEAPSHGMPVMAYDAQAKGTKAYLALADELMARVSGK"
BASE COUNT          441 a          501 c          748 g          509 t
ORIGIN      
        1 aattcggtct gcttcggaag agggggacgg cgatgtttgt gaacgagaaa tatccctatg
       61 cggctttgtt tgcggggcta gtgtttttga cgctgccgtt cgcactgtcg gtgcatgatg
      121 ccttggcatt tgcgttcgga cgggcggggc tgctggtgtc ggtgtcggac ggcggattcg
      181 gctggcgtgg cggttgggac ggcactgttt ggtttgtgtt cggtgtgttt gcgtttttga
      241 atgtggttgt atcggcgggt ctgacgaaac tggcgtacaa aaagatgatg cggcggcatt
      301 cccgttacgc gctgtttctg tcgggcgtgg cggcttgcgc ggcagcagcg gtggcttgga
      361 ttttcgagct gttgttgggc agtgaggctt tgggcgggct gcgcggggag gcggtgtcgg
      421 aatatgcgtt tgccgtgtgg ctggtggcga tgctgacgct gcccaaacgc ctgacgcgcg
      481 cgccggtgca gccggtggtg tttcacagga aaaaataggt tggaacggga atgccgtctg
      541 aaacgccgac acgcggtttc agacggcatg tttttccgtt aacattacgc ctgaatatgg
      601 ataggaagca gatatggaac gtaaagaacg cctgcgtgca ggcattgccg cgatggggct
      661 ggatattttg gaaacggcgc aggacaggct tttggcctat gtggatttgt tgaaaaagtg
      721 gaacaaaacc tacaatctga ccgcactgcg cgacgaggaa aaaatgattg tccatcatct
      781 tttggacagc ctgacgctgc tgccctatat cgaaggcgca cagacgatgc tggatgtcgg
      841 ttcgggcggc ggccagcccg gcattccggc ggcggtgtgc cgtccggatg tgcagataac
      901 ccttttggat gcgaatacga agaaaacggc ttttttacag caggcggtta tcgagttggg
      961 attggacaat gtgcgcgtgg tatccggacg cgtggaggcg gtttcggacg tgcgtgccga
     1021 cgtgattacc agccgtgcgt ttgcagaact ggcggatttt gtgtcgtgga cggggcatct
     1081 gttgaaagac ggcggctact gggcggcgat gaagggcgtg tatccgcagg gggaaatcga
     1141 ccgcctgccg caggatgtgt gcgtcgaaaa agtccaaagg cttgacgtgc cgggcttgga
     1201 tgcggaacgc catatcgcca tactccgcaa gcgttgagcg cgcttcagac ggcatgaata
     1261 ccttttttgt gcggataaag gtaaaattcc gcactgtttt tcttttttca acatcagacg
     1321 ggacacgggc gggacatgag tgcgaacatc cttgccatcg ccaatcagaa gggcggtgtg
     1381 ggcaaaacga cgacgacggt aaatttggcg gcttcgctgg catcgcgcgg caaacgcgtg
     1441 ctggtggtcg atttggatcc gcagggcaat gcgacaacgg gcagcggcat cgacaaggca
     1501 agtttgcagt ccggcgttta tcaggtcgta ctgggcgatg cggacgtgaa atcggcgacg
     1561 gtacgcagca aagagggcgg atacgctgtg ttgggcgcga accgcgcgct ggccggtgcg
     1621 gaaatcgagc tggtgcagga aatcgcccgg gaagtgcgtt tgaaaaacgc gctcaaggca
     1681 gtggcggaag attacgactt tatcctgatc gactgtccgc cttcgctgac gctgttgacg
     1741 cttaacggct tggtggcggc gggcggcgtg attgtgccga tgttgtgcga atattacgcg
     1801 ctggaaggga tttccgattt gattgcgacc gtgcgcaaaa tccgtcaggc ggtcaatccc
     1861 gatttggaca tcacgggcat cgtgcgcacg atgtacgaca gccgcagcag gctggttgcc
     1921 gaagtcagcg aacagttgcg cagccatttc ggggatttgc tttttgaaac cgtcatcccg
     1981 cgcaatatcc gccttgcgga agcaccaagc cacggtatgc cggttatggc ttatgatgcg
     2041 caggcaaagg gtaccaaggc gtatcttgcc ttggcggacg agctgatggc gagggtgtcg
     2101 gggaaatagg tcaatccaaa tcaggctgcc cgtgccttta tgccgtttga ccgagtgcgt
     2161 taatatggtg gattaaaata aaaatactta tatcgtcat
//
LOCUS       DAWFHY010000150         2165 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.150, whole genome shotgun sequence.
ACCESSION   DAWFHY010000150 DAWFHY010000000
VERSION     DAWFHY010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2165
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.150"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            464..1921
                     /locus_tag="WGQ68_002035"
     CDS             464..1921
                     /locus_tag="WGQ68_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEZ0715763.1"
                     /translation="MSRHPAPTGEKTFFGHPFQLSTLFHIELWERFSFYGMQGILLIY
                     LYYTADKGGLGIDKTLAGGIVGAYSGSVYLSTILGAWFADRVWGAEKTLFLSGIVVML
                     GHIVLAAAPGLYGLLIGLIFIALGSGGVKSTASSMVGALYEQDEMRPLRDAGFSIFYI
                     AINIGGFLGPLLTGLLQENIGFHYGFGAAAVGMAFGLWRYSLGRKNLPHPTVPHPLSK
                     GQGKTAAAVGIALIAALATAIKTGLVNLDNFSGILLSTVILAVIAYFARLLTNPRVSS
                     DNKRHIIAYIPLFLTICMFWAVWFQIYTVATVYFDETVNRTIGSFTVPVAWKDSMQSL
                     WVILFSGLMAAMWTKMGRKQPKTPLKFAMAVFVTGASFLGFVPFISAGTPMPIAVFAL
                     IVLAITIGELMISPIALSISTKIAPPLFKTQMVALNFLAFSLGFTLGGVLFEKGYQAG
                     DEIGFYRLLFYIGAATGFLLLLLVPKLNKMLEGTD"
BASE COUNT          502 a          629 c          497 g          537 t
ORIGIN      
        1 gacggtcttt atagcggatt aacaaaaatc aggacaaggc ggcgaagccg cagacagtac
       61 agatagtacg gaaccgattc acttggtgct tcagcacctt agagaatcgt tctctttgag
      121 ctaaggcgag gcaataccgt actggttttt gttaatccac tataatgcgc ccttcgacgt
      181 ggcggatata taaggaagtg attttccatc taagtaaaaa ccgccctatc ggataagccc
      241 ttaacagaaa aggctttacc cgcgccgtat cggaacacat cctctaaaat acaatccgtt
      301 gaattgaaaa aaatataaaa acatccgccc gcgaaaaacg gcagcgcgtc gtttgacaaa
      361 gattgaaaat atcggttaaa aaccgatttt catacaaaaa acaccgctgc cgtccgcatc
      421 cgtttcagac ggtattgaga gaaaatcttt taggagaacc tttatgtccc ggcatcccgc
      481 ccccaccgga gaaaaaacat tcttcggcca ccccttccag ctttccaccc tcttccatat
      541 cgaattgtgg gaacgttttt cattttacgg aatgcagggc atcctgctga tttacctcta
      601 ctacaccgcc gacaaaggcg gcttgggcat agacaaaacc ctcgccggcg gcattgtcgg
      661 cgcatacagc ggcagcgtgt acctgtccac cattttgggg gcgtggtttg ccgaccgagt
      721 atggggtgcg gaaaaaaccc tcttcctctc gggcatcgtc gtgatgctcg gacacatcgt
      781 ccttgccgcc gccccgggcc tgtacggcct tttaatcggg ctgatattca tcgcattggg
      841 cagcggcggc gtgaaatcta cggccagttc tatggtgggc gcattatacg aacaagacga
      901 aatgcgcccg ctgcgcgatg cgggattttc cattttctac atcgccatca atatcggcgg
      961 cttcctaggc ccgctgctga ccggcctgct tcaagaaaac atcggtttcc attatggttt
     1021 cggcgcggca gcggtcggta tggcattcgg cttgtggcgt tattccttgg gacgtaaaaa
     1081 cctgccccac cccaccgtcc cccatccgct ttcaaaagga cagggcaaaa ctgcggccgc
     1141 cgtcggcatt gctctcatcg ccgcacttgc aaccgccatc aaaacggggc ttgtcaacct
     1201 cgacaatttc tccggcatcc tcttatctac cgtcatcctt gccgtcattg cctatttcgc
     1261 ccgcctgctg accaaccccc gcgtcagttc cgacaacaag cggcacatca tcgcctacat
     1321 cccgcttttc ctgaccatct gtatgttttg ggccgtctgg tttcagattt acaccgtagc
     1381 aaccgtctat ttcgacgaaa ccgtcaaccg caccatcggt tcgtttaccg tgcccgtcgc
     1441 ctggaaagat tccatgcaaa gcctgtgggt catcctgttt tccggactga tggcggcaat
     1501 gtggacaaaa atggggcgca aacagcccaa aaccccgctg aaattcgcta tggcggtatt
     1561 tgttaccggc gcgtcgtttt tgggattcgt cccctttatt tccgccggca cgccgatgcc
     1621 tattgcggtt ttcgcactga ttgtccttgc catcacgata ggcgaactga tgatttcccc
     1681 gattgcgctg tccatctcca ccaaaatcgc accgccttta ttcaaaaccc aaatggtcgc
     1741 ccttaatttc cttgcctttt cattaggctt cactttgggc ggcgtattgt ttgaaaaagg
     1801 ctatcaggcg ggcgacgaaa tcggcttcta tcggctgctg ttctacatcg gcgcagccac
     1861 aggcttcctg ctgctcctgc tcgtccccaa attgaacaaa atgctcgaag gcacagacta
     1921 agtcccgccc cgatgccgtc tgaacccttc agacggcatt tttccgcata atgaaaccaa
     1981 accgtttcca cccgacagga caggctcccc ccaaccggaa ggcagcctgc cgattgtcat
     2041 ttaaatatct gactgattcc gtctgtttgc aagggaaata gccgtttatt tccgcttggg
     2101 cggaaaccgg ttctattgag taaaaggcat tttatccgac taaattagtg ctgcatcaac
     2161 ggaat
//
LOCUS       DAWFHY010000151         2151 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.151, whole genome shotgun sequence.
ACCESSION   DAWFHY010000151 DAWFHY010000000
VERSION     DAWFHY010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.151"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            144..530
                     /locus_tag="WGQ68_002036"
     CDS             144..530
                     /locus_tag="WGQ68_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014573928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0715764.1"
                     /translation="MKGLDKYILFKNCETLGELFRIYQDYLSLNIDDPNLKIKFFDLL
                     EELLSDGVIELCDYRENPPKILTGSPKTQVDELRKIWPDMEEMLLYFSDNPWHYVEWF
                     WWGATCPIELTQLPKIEIYEEQMKQA"
     gene            558..2151
                     /locus_tag="WGQ68_002037"
                     /pseudo
     CDS             558..2151
                     /locus_tag="WGQ68_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
BASE COUNT          769 a          483 c          496 g          403 t
ORIGIN      
        1 ccttggaaag gaacgaccat cattttaagg aatttttcaa ctagccgaga acaaactgga
       61 gcaaaatgga cgattgagtt tcaaaatcat ccattagtag gtaggaaaaa aatggaatta
      121 aaatttagat aaggaattga ataatgaaag gtttagataa gtatatccta tttaaaaatt
      181 gtgaaacatt aggagaattg tttcgaatat atcaagacta tctatcatta aatattgatg
      241 acccaaattt aaaaattaaa ttttttgacc ttttggaaga acttttatca gatggtgtta
      301 ttgaattatg cgattatcgt gaaaatcctc caaaaatatt aaccggttcg ccaaaaacac
      361 aggtagatga attaagaaaa atttggccgg atatggaaga gatgttgcta tatttttcag
      421 ataacccttg gcattatgtt gaatggtttt ggtggggagc aacttgccct attgaactga
      481 cacaattgcc taaaatagaa atttacgaag aacaaatgaa acaagcttaa agccagcgta
      541 accagcctgt atgacaaggt gcaggtgaac aatccaatat ccacattacc ggctcagatg
      601 ttgccggcaa agaaggaacc ggcctgattg cagataagca aatccatctg caatcagccg
      661 agcaaagtaa taccgaacgc agccaaaaca aatcggcagg ctggaacgca ggtgctgccg
      721 tatcattcgg acaaggaggc tggtcattag gcgttaccgc aggcggcaat atcggcaaag
      781 gccacggcaa tggcgacagc atcacccacc gccatagcca tatcggcgac aaaggcagcc
      841 aaacccttat ccaaagtggt ggcgatacta ccatcaaagg cgcgcaagta cgcggcaaag
      901 gcgtacaagt caatgccaaa aacctaagta ttcaaagcgt acaagatagc gaaacctatc
      961 aaagcaaaca acaaaacgcc agtgcacaag tgaccgtcgg ttatggcttc agtgccagtg
     1021 gcgattacag ccaaagcaaa atccgagcca atcatgcttc ggtaaccgag caaagcggta
     1081 tttatgccgg agaagacggc tatcaaatca aggtcggaaa ccatacagac ctcaaaggcg
     1141 gcatcatcac cagtacccaa agcgcagaag acaagggtaa aaaccgattc agcacaggca
     1201 cactcgccgg cagtgatatt caaaattaca gccaatacga aggaaaaagt tttggattgg
     1261 gcgcaagtgc gtccataagc ggcaaaacac tgggacaggg cgcaaaaaat aaacctcaaa
     1321 acaaacacct gacaagcgta gccgataaaa acggcgcaag ttcatcagta ggctacggca
     1381 gcgacagcga cagcgacagc caaagcagca tcacaaaaag cggcatcaat acccaaaaca
     1441 ttcaaatcac cgacgaagcc gcacaaatcc ggctgacagg aaaaacagcg gcacaaacca
     1501 aagccgatat tgatacaaac gtaaccacag acaccgccga acgacattcg ggcagcctga
     1561 aaaacgtatt tgacaaagat agagtgcaaa gtgaactgga tttacaaaga accgtcagcc
     1621 aagattttag taaaaatgtt caacaagcca ataccgagat taaccaacat ttagacaaac
     1681 tcaaagcaga caaagaagca gccgaaacag cagcagccga ggcattagcc aatggcgata
     1741 tggaaactgc caaacacaaa gcccatgaag ctcaagatgc ggcagcaaaa gcagataatt
     1801 ggcaacaagg caaagtcatt ctcaacatgt tagcctcagg tttagctgcg ccgacccaaa
     1861 gcggagcggg catcgctgcg gctaccgcat cgccagccgt atcgtatgcg attggacagc
     1921 actttaaaga tttagccggt caaaacgcga atggcaaact aaccgccagt caagaaaccg
     1981 ctcacgttct tgcccacgcg gtattaggag cagcggttgc agcagcagga ggcaacaatg
     2041 ccccggcggg agcattgagt gcgggcgggt cggaagcggc tgcgccttac atcagcaaat
     2101 ggttatatgg caaagaaaaa ggaagtgact taacggcgga agagaaagag a
//
LOCUS       DAWFHY010000152         2128 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.152, whole genome shotgun sequence.
ACCESSION   DAWFHY010000152 DAWFHY010000000
VERSION     DAWFHY010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.152"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(336..782)
                     /locus_tag="WGQ68_002038"
     CDS             complement(336..782)
                     /locus_tag="WGQ68_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715765.1"
                     /translation="MIFEDYFSELQADMVSICLEYVQGKADKIYIYAAFEPRVSGAGC
                     FFSVKGKLVQRHLVNQEIPEVNLDHQIQVLDILMEDLEKLNSICQKFNQPMPTEIKMI
                     YDVKKNSLSANYQYNLILSSESGLLALPQDVEDAWFEEIKKQMEDN"
     gene            complement(797..>2128)
                     /locus_tag="WGQ68_002039"
     CDS             complement(797..>2128)
                     /locus_tag="WGQ68_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEZ0715766.1"
                     /translation="TGGSGGAVVGANVDWHNRQLHPSERDWIRKNAQAFAKQEVGGRE
                     PTIDEIALAEKRLAQQAAKETDLFWMLTLEKVTDLSAQKFLRQAKGTFTNENGTQQQF
                     FTTQGKQFMRPELFANEVYDLSFYKNHLQSPKNSSIGKGTGELARRVTSSTWDKAKQD
                     PILTGLQIAASPVHLVRDVGKASWQCVSGFGQCLQQGKENFVETGSAIGTGTASFQLN
                     DLKPIYGQDVRGAQAGLVALQTADGITTALGAAKALNVAGKGSRYFINEWASSPPSGS
                     RAAQRGAVGNLTHSQQVLTDGSHIQRGGKLLPNIRYKAGEFDYLYETDTLGRIKSFET
                     DNLQLTQRDRLKHNSKTPDKVKGQDHAGHLIGDRFGGSPNLDNLVSQHKDVNLSAYKV
                     LENKWAEAIKSGKNVKIQMQIEYAAGTRRPTRFVGYYWIDGKPTRININNL"
BASE COUNT          612 a          456 c          323 g          737 t
ORIGIN      
        1 ttgtctaatt ttcaaaatta gctcagttgc attagattga ttatatccta gagctgattc
       61 aaaaactcta gctttatgtc tcccaacatc atgattagga ttcaatgcat aatctgtaaa
      121 ttttctggta tcgattatga cagaatcagc gttagggatt agcaagcgta gggtgggcaa
      181 ctcgttgccc acgcggtttg atacacgcca cgttatttta ttttttcagg cagcctgaaa
      241 tgaaatattt cacattaaaa catcatttta acatatacga ttgaactgcg tgggcaacga
      301 gttgcccacc ctacgcttgc tacgcttgct gtgaattaat tatcttccat ttgcttctta
      361 atttcttcaa accatgcatc ttcaacatct tgcggtaaag caagtaatcc agattcagat
      421 gacaagatta aattatattg ataatttgct gataagctat tcttttttac atcataaatc
      481 attttaattt cagttggcat aggttggtta aatttctgac aaatagaatt tagtttttct
      541 aaatcttcca taagtatgtc taatacttga atttgatggt caagattaac ttctggaatt
      601 tcttgattaa caagatgtcg ttgtacaagt tttcctttaa cagagaaaaa acatccagca
      661 ccactaacac gaggctcaaa agctgcataa atgtaaattt tatcagcctt accttgaaca
      721 tattccaaac aaatagaaac catatctgct tgtaattcac taaaataatc ttcaaaaatc
      781 ataaaaaatc cttgtttcat aaattattaa tattaattct agttggttta ccatcaatcc
      841 aataatatcc aacaaatcgt gtagggcgtc ttgtacctgc tgcatattca atttgcattt
      901 ggattttaac attttttcct gatttaattg cttccgccca tttattctcc aaaactttat
      961 acgcacttaa gtttacatct ttgtgctgtg acactaaatt atctaaattg ggcgaacctc
     1021 caaatctatc tccaattaaa tgtcctgcat ggtcttgccc cttgacttta tcaggagttt
     1081 ttgaattatg ctttaatcta tctctctgtg ttaattgcaa attatctgtt tcaaaacttt
     1141 taattctacc taaagtatca gtttcatata aataatcaaa ctcgcctgct ttatagcgaa
     1201 tattaggtaa aagttttccg ccacgttgaa tatgtgaacc atctgtcaaa acttgttgcg
     1261 aatgggttaa attccccact gccccacgct gtgcagcacg agaaccacta ggcggactac
     1321 tagcccattc atttataaaa tagcgactac cttttcctgc aacatttaat gcttttgctg
     1381 cacccaatgc cgtagttatt ccatctgccg tttgtagtgc aaccaatcct gcttgtgcac
     1441 cacgtacatc ttgaccataa atcggcttaa ggtcgtttag ttgaaaagag gcagttcctg
     1501 ttccaattgc agaacctgtt tctacaaaat tctcttttcc ttgttgcaaa cattgtccaa
     1561 agccgcttac gcactgccag cttgccttac ctacatcccg aactaaatgt acaggggagg
     1621 cagctatttg caatcctgtc aaaataggat cttgttttgc tttatcccaa gtagatgaag
     1681 taactcttcg agccaattct cctgtaccct tgccgatact actattttta ggagattgta
     1741 aatggttttt gtaaaaagac agatcatata cttcattagc aaacaattca ggacgcataa
     1801 attgcttccc ttgcgtagta aaaaattgtt gctgcgtacc attttcattt gtaaaagttc
     1861 cttttgcttg tcttaggaat ttttgagctg ataaatctgt tactttttct aatgttagca
     1921 tccaaaacaa atctgtctct ttagccgcct gttgtgctag tcgtttttct gctaatgcaa
     1981 tttcatctat agtgggttct ctaccaccta cctcctgttt cgcaaaagct tgtgcatttt
     2041 ttcttatcca atctctttca ctcggatgca actgccgatt atgccaatct acattcgcac
     2101 ccaccacagc accaccacta ccaccagt
//
LOCUS       DAWFHY010000153         2032 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.153, whole genome shotgun sequence.
ACCESSION   DAWFHY010000153 DAWFHY010000000
VERSION     DAWFHY010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.153"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(353..1462)
                     /locus_tag="WGQ68_002040"
     CDS             complement(353..1462)
                     /locus_tag="WGQ68_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEZ0715767.1"
                     /translation="MSNWKPDIPYNDLPPLPPKQDIESKTILKRCIAARASLARLKQA
                     AELIPNQAMLINTLPVMEARASSEIENIVTTTDKLFQSLQMDTERQDPATKEALQYRT
                     ALFAGYESLTSRPLCTQTAIMVCNAIKHPYEMAIRKTGGTALKGGNSGNVVYTPPEGE
                     ETIRGKLANWERFIHESGDLDPLIIMAAAHYQFEAIHPFTDGNGRTGRILNSLLLIEK
                     GLLDLPILYLSRYIIENRADYYRLLLGVTERQDWESWIIYILDGVADTADWTVSKIDA
                     IRRLFEQTRQHIRTHAQGIYTHELVNLLFEQPYTRIANLEAAGIAKRQTASKYLKELS
                     DIGVLQEIVIGRDKLFIHPRLMELLRGEGNSFTSF"
BASE COUNT          498 a          439 c          484 g          611 t
ORIGIN      
        1 ctgtgtttta acgatgtgtt gatatttaat tttagaaagg tagctattta atagttacct
       61 tttcttattt aaaaatagct ttctcaaatt ccatgaacgc ctcaatacga tatgcagatg
      121 ctctatcgaa attaaggttc aacattttgt ttattaaaca ttttatttta gccattttca
      181 atataccccc aaatataccc ccaatttgca caagtcaaaa ttttaggcag gggttttgtt
      241 gctcccatga tacgcgggcg gatgccggct tttcgcgggg gaaatacaag gggtctcggt
      301 tcgggtgtca aaatccctgt ttcgtgttag tcatgtgggg gggaagaggg ggttagaatg
      361 aagtaaagct gttgccctct ccccgcaata gttccattag gcgcggatga atgaatagtt
      421 tgtccctgcc gatgacgatt tcttgcagca cacctatgtc tgaaagctct ttcaggtact
      481 tagaggccgt ctgccgtttg gctatccctg ccgcttctag gttggcaatg cgtgtatatg
      541 gctgctcaaa cagaagattt accagttcgt gcgtgtagat tccttgtgcg tgtgtccgta
      601 tgtgttgccg tgtctgctcg aacaggcggc gtatcgcatc tattttcgat accgtccaat
      661 cggcggtgtc ggctacgccg tctaagatgt agattatcca gctttcccag tcctgccgtt
      721 cggttacgcc taacagcagg cggtaatagt ccgccctgtt ttcgatgatg tagcggctca
      781 aatacaaaat aggcaaatcc aaaagccctt tttcaatcaa tagcaggctg ttcaatatgc
      841 gccccgtccg cccgttgccg tccgtaaacg gatggatggc ttcaaattgg taatgtgccg
      901 ccgccatgat gataagcggg tctaaatcgc cgctttcgtg aataaaccgc tcccaatttg
      961 ccagcttgcc gcgtatggtt tcttctcctt cgggcggggt atagacaaca tttccgctgt
     1021 tgcctccttt tagggctgtg ccgcctgttt tgcggatggc catttcgtag gggtgcttga
     1081 tggcgttgca gaccatgatg gcggtttgtg tgcataaagg gcggctcgtc agtgattcat
     1141 agcctgcaaa cagggcggtg cggtattgca gggcttcttt cgtggcaggg tcttgccgtt
     1201 ccgtatccat ttgcagggat tgaaacagct tgtccgttgt ggttacgatg ttttcaattt
     1261 ccgaacttgc acgggcttcc ataacaggaa gggtgttaat cagcatggct tgattcggta
     1321 tcaattctgc cgcctgcttt aaacgggcaa gggatgcacg ggcggctata caacgtttca
     1381 ggatggtttt gctttcaata tcctgttttg gcggcagggg tggtaaatcg ttatagggaa
     1441 tatcgggttt ccagttgctc atatttaaaa tttcaaaaaa tttaaagatg tttccagtat
     1501 atgtttacgc cgtgtatata tcaaggatat atgtttaaaa atttggcttt tgtaagtata
     1561 tgggaggtaa aaccgcccgc aaaagtcaat ttgcatgggg ttaattcaaa atccgccctt
     1621 catctgcccg cttccctaaa aaaaaccgta tatatataac tgtctgcatt ctatcgctcc
     1681 ggcgacgata cccatatttc caagtttgtg tatcaaaatt gtatatcggg catagactat
     1741 ttcggcgagg acgaagatat agatttccac gattgaatac atggaagcca agtacgtcta
     1801 tcaacactat attaaaacac agcctttttt ttgaggtttc gggtaacttt taaaccgtca
     1861 ttcctacgaa aacagaaaat caaaaacaga aatctcaaat cccgtcattc ccgcgcaggc
     1921 gggaatctag acattcaatg ctaaggcaat ttctcggaaa tgactgaaac tcaaaaaact
     1981 ggattcccac tttcgtggga atgacggaat gtaggttcgt gggaatgacg tg
//
LOCUS       DAWFHY010000154         1980 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.154, whole genome shotgun sequence.
ACCESSION   DAWFHY010000154 DAWFHY010000000
VERSION     DAWFHY010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.154"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(126..705)
                     /locus_tag="WGQ68_002041"
                     /pseudo
     CDS             complement(126..705)
                     /locus_tag="WGQ68_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002256969.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
     gene            complement(1018..1290)
                     /locus_tag="WGQ68_002042"
     CDS             complement(1018..1290)
                     /locus_tag="WGQ68_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715768.1"
                     /translation="MNKRMKMCPACQQGYLYHSKPKYLHDEIILCDECDAVWLKGMNI
                     FYGEYEKDFYSYVPFMESQGITSECIWEGDLFDHPYYEDENSNGMD"
     gene            complement(1478..1699)
                     /locus_tag="WGQ68_002043"
     CDS             complement(1478..1699)
                     /locus_tag="WGQ68_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002251499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715769.1"
                     /translation="MAVWRDYITLESHDLAEGYTYFFNENTDDCYVLKQDFINNERYE
                     KTELYSQKDKVILFPKFGEYDLVLNPDII"
     gene            complement(1747..1965)
                     /locus_tag="WGQ68_002044"
     CDS             complement(1747..1965)
                     /locus_tag="WGQ68_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004520563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715770.1"
                     /translation="MKKDIFYCEQWSYGYKRLHKPFSEKQAEEKHLKGELYTAVIGSA
                     TQPEYVITLREEVGFFSVNFSINLEGII"
BASE COUNT          633 a          414 c          294 g          639 t
ORIGIN      
        1 aaaaatattt tcttccaaac tcaacttgta tttgtccgta accacgagag ttcctttaat
       61 aattaaatct gtacacccaa ggtatgcacg gtaaacggtg ctatattaaa aagacccatt
      121 aaaggttaaa caaaaagtcg cttgtcccgt tcatggctct caatattttt caaggcatcc
      181 gccttgtccc atatctgaag ttcccacgga aaaaacagat tatttttttg attaaaatac
      241 acatgcgttc cgatatagcc gttttttttc ctgaacgttc cagtttttta agcccttcgt
      301 cgaaacccat tcatctaaag cagcatgtat ttcttccatt agtgcggaat ctgcgatgat
      361 tctcacccca aaaatatcat ttaacacctt attcgctgga tacccggact cgcgttccga
      421 aaaccagcgg attttgtcga aaacggactc cgtggtttta acgcggtagt agtatgccaa
      481 aggcaagtcc ccgatgtcag atcgaatcag atagtcgttg attgactcat gcaaattaag
      541 tcggtattgc aacacaaaat cgatgtccag atgccggagc gtctttgaca aattgggaag
      601 gtttccgcca tacagtttgg aaaccctttc gtgatatccg gcgtgtatgc ggttaatttc
      661 gtcaatcagc cgttctacat tactgatttc atgagtgggg gacattcctt gttactcttt
      721 cattccaatt atatgaaaat aacatactaa ttgctttcaa ctatatatta tacaaaacaa
      781 atttcaaaac ttccttttaa tttttcatta ccgttaatac gctaaaatag gaggaattgc
      841 aaatccaaaa aacatacacg gtaacagatt acaggtcatc aatgaatcca atccatccca
      901 tgaagacaaa ggagcgacta ctcggcctcc aagcagcctc ccgtactgaa aacgacattg
      961 cattaaagac agcgccgttt gatggctaat cgtacctacg caggcttaaa gtttccatca
     1021 atccatacca tttgagtttt catcttcata atatggatga tcaaacaaat ctccttccca
     1081 aatacattca ctcgttatac cttgggattc catgaaagga acataagaat aaaaatcttt
     1141 ttcatattct ccataaaata tattcatacc tttgagccat actgcatcgc attcatcaca
     1201 cagaataatt tcatcatgaa gatatttagg tttcgaatgg tagagatagc cttgttgaca
     1261 agcaggacac attttcattc tcttattcat gattattcca tataatcaat ttatttttgt
     1321 gggtaagcaa tagtgatttg attgctgcct ttagtctgta caacaatacg tatatagcaa
     1381 tctgataatt tcctggtttt tatcagcttt atcctcagat tccaaaccca ataataaaag
     1441 ccgtttgaaa tttttttcag acggccttaa aacttaatta aataatgtcc ggatttaaca
     1501 ccaaatcata ttcaccaaac tttggaaata gaattacctt atctttttgg gaatataatt
     1561 ctgttttttc atatcgctca ttattaataa aatcttgttt caaaacatag caatcatccg
     1621 tattttcatt gaagaaataa gtatatcctt ctgctaagtc atgagattcc aaagttatat
     1681 aatctctcca tacagccata gaaagaaaat aataattcga attggaatat ttttgaaatt
     1741 gatgggttaa ataatccctt ccaaatttat cgaaaaattt accgaaaaaa aacctacttc
     1801 ctctcgcaag gtaattacat attcaggttg tgtcgccgaa cctattacgg cagtatataa
     1861 ctccccttta agatgttttt cctcagcttg tttctcagaa aaaggcttat gaagtctctt
     1921 ataaccataa gaccactgct cacaataaaa aatatctttc ttcatttgta aatccttcct
//
LOCUS       DAWFHY010000155         1941 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.155, whole genome shotgun sequence.
ACCESSION   DAWFHY010000155 DAWFHY010000000
VERSION     DAWFHY010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.155"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            75..1016
                     /gene="mafA"
                     /locus_tag="WGQ68_002045"
     CDS             75..1016
                     /gene="mafA"
                     /locus_tag="WGQ68_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin MafA"
                     /protein_id="HEZ0715771.1"
                     /translation="MKTLLLLIPLVLTACGTLTGIPAHGGGKRFAVEQELVAASSRAA
                     VKEMDLSALKGRKAALYVSVMGDQGSGNISGGRYSIDALIRGGYHNNPESATQYSYPA
                     YDTTATTKADALSSVTTSTSLLNAPAAALTKNSGRKGERSAGLSVNGTGDYRNETLLA
                     NPRDVSFLTNLIQTVFYLRGIEVVPPEYADTDVFVTVDVFGIVRSRTELHLYNAETLK
                     AQTKLEYFAVDRDSRKLLIAPKTAAYESQYQEQYALWMGPYSVGKTVKASDRLMVDFS
                     DITPYGDTTAQNRPDFKQNNGKNPDVGNEVIRRRKGG"
     gene            1020..>1941
                     /gene="mafB"
                     /locus_tag="WGQ68_002046"
     CDS             1020..>1941
                     /gene="mafB"
                     /locus_tag="WGQ68_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0715772.1"
                     /translation="MKPLRRLTNLLAACAVAAAALIQPALAADLAQDPFITDNAQRQH
                     YEPGGKYHLFGDPRGSVSDRTGKINVIQDYTHQMGNLLIQQAAIQGNLGYTVRFSGHG
                     YEEHAPFDNHAADSASKEQGNVNEGFTVYRLNWEGHEHHPADAYDGPKGGNYPKPTGA
                     RDEYTYHVNGTARSIKLNPTDTRSIRQRISDNYNNLGSNFSDRADEANRKMFEHNAKL
                     DRWGNSMEFINGVAAGALNPFISAGEALGIGDILYGTRYAIDKAAMRNIAPLPAEGKF
                     AAIGGLGSVAGFEKNTREAVDRWIQENPNAA"
BASE COUNT          504 a          708 c          435 g          294 t
ORIGIN      
        1 ttcgcccctt ttgcttacaa acccttaaaa tccctttaca ctcaaaatcc gttcaacatc
       61 aaacaaaccc cgctatgaaa accctgctcc tcctcatccc cctcgtcctc accgcctgcg
      121 gcacactgac cggcattccc gcccacggcg gcggcaaacg ctttgccgtc gaacaagaac
      181 tcgtcgccgc atcgtcccgc gccgccgtca aagaaatgga tttgtccgcc ctgaaaggac
      241 gcaaagccgc cctttacgtc tccgttatgg gcgaccaagg ttcgggcaac ataagcggcg
      301 gacgctactc tatcgacgca ctgatacgcg gcggctacca caacaacccc gaaagtgcca
      361 cccaatacag ctaccccgcc tatgacacta ccgccaccac caaagccgac gcgctctcca
      421 gcgtaaccac ttccacatcg cttttgaacg cccccgccgc cgccctgacg aaaaacagcg
      481 gacgcaaagg cgaacgctcc gccggactgt ccgtcaacgg cacgggcgac taccgcaacg
      541 aaaccctgct cgccaacccc cgcgacgttt ccttcctgac caacctcatc caaaccgtct
      601 tctacctgcg cggcatcgaa gtcgtaccgc ccgaatacgc cgacaccgac gtattcgtaa
      661 ccgtcgacgt attcggcatc gtccgcagcc gtaccgaact gcacctctac aacgccgaaa
      721 cccttaaagc ccaaaccaag ctcgaatatt tcgccgtcga ccgcgacagc cggaaactgc
      781 tgattgcccc taaaaccgcc gcctacgaat cccaatacca agaacaatac gccctctgga
      841 tgggacctta cagcgtcggc aaaaccgtca aagcctcaga ccgcctgatg gtcgatttct
      901 ccgacatcac cccctacggc gacacaaccg cccaaaaccg tcccgacttc aaacaaaaca
      961 acggtaaaaa ccccgatgtc ggcaacgaag tcatccgccg ccgcaaagga ggataaaccg
     1021 tgaaaccgct gcgcagactg acaaacctcc ttgccgcctg cgccgtagcg gcggccgcac
     1081 tcatacagcc cgccctcgcg gcggacttgg cgcaagaccc gttcattacc gataacgccc
     1141 aacggcagca ctacgaaccc ggaggcaaat accacctctt cggcgacccg cgcggcagcg
     1201 tttccgaccg caccggcaaa atcaacgtca tccaagacta tacccaccag atgggcaacc
     1261 tgctcatcca acaggcggca atccaaggca atcttggtta caccgtccgc ttttcaggac
     1321 acggatacga agaacacgcc cccttcgaca accatgccgc cgacagcgca agcaaagaac
     1381 aaggcaacgt caacgaaggc tttaccgtat accggctcaa ctgggaagga cacgaacatc
     1441 atcccgccga tgcctacgac ggcccgaagg gcggcaatta ccccaaacct acgggtgcac
     1501 gcgacgaata cacctatcac gtcaacggca cagcccgcag tatcaaactc aatccgaccg
     1561 acacccgcag catccggcaa cgcatatccg acaactacaa caacctcggc agcaatttct
     1621 ccgaccgcgc cgatgaagcc aacagaaaaa tgttcgagca caatgccaag ctcgaccgct
     1681 ggggcaacag catggagttt atcaacggcg tcgccgccgg cgcgctcaac ccctttatca
     1741 gcgcgggcga agccttgggc ataggcgaca tactgtacgg aacgcgctat gccatagaca
     1801 aagccgcaat gcgcaacatc gcccccttgc ccgccgaggg caaattcgcc gccatcggcg
     1861 gcttgggcag cgtggcgggc tttgaaaaga atacgcgcga agccgttgac cggtggatac
     1921 aggaaaatcc caatgccgcc g
//
LOCUS       DAWFHY010000156         1888 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.156, whole genome shotgun sequence.
ACCESSION   DAWFHY010000156 DAWFHY010000000
VERSION     DAWFHY010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1888
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.156"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(124..552)
                     /locus_tag="WGQ68_002047"
     CDS             complement(124..552)
                     /locus_tag="WGQ68_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mor transcription activator family protein"
                     /protein_id="HEZ0715773.1"
                     /translation="MAFEKVEHLLPDTVLDIVDVIGLAATEQLVKAIGGARFKFGKGK
                     VDTERLAILVEAIGEVKTHELLQVYGGEELYVPRCGKALIQLRNHRFYQEFVKLRDID
                     KESGLMAMTKLCPKYGISSRTGYTIINEMSRPAAQQAALF"
     gene            complement(533..967)
                     /locus_tag="WGQ68_002048"
     CDS             complement(533..967)
                     /locus_tag="WGQ68_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gp16 family protein"
                     /protein_id="HEZ0715774.1"
                     /translation="MRRALIAKIKIAQKELGLDDGTYRAVLERVTGKRSCADMDVSEL
                     ESVVADMRSHGFKPKAKGNPHGKPHLRRTSSAAMLDKVEALLTVGGKHWNYAHAMARR
                     MFGKDKVEYLDDTQLHKLVAALQIAENRKTEKAGGDDGVRKS"
     gene            complement(1124..>1888)
                     /locus_tag="WGQ68_002049"
     CDS             complement(1124..>1888)
                     /locus_tag="WGQ68_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEZ0715775.1"
                     /translation="RRAGIAVIIANPRQTHQFAQSQPLTKTDAKDAKMPAFFTQMMTQ
                     KEDWQTMPYQPPTEAEEVLEALVNRRNQSADMRTAEKNRLHQVHETQVGSVKQLIAHF
                     DRLIDELDKQIDNHTHTHFDGKAQVAEQIKGIGSITTATLMAMLPELGRLSHKRIASL
                     VGIAPHPRESGETKFKSRCFGGRSAVLKALYMATVAATRFEPLIRDFHQRPLSEGKPY
                     KVAVTACMRKLLTISNARMRDYFAENDTAENGIRTA"
BASE COUNT          442 a          542 c          411 g          493 t
ORIGIN      
        1 atttgaaaac cccattaaac cgtctttaac ccatcgtcct gaacggtaga tgtttcaccc
       61 cctgcgaacc aaaaaaaaga ccgtctgaat acagacggcc aaagcctggt cacacatcac
      121 tgcctaaaat aaagctgcct gctgtgccgc aggtcggctc atttcattga taatcgtata
      181 tcccgttcgt gaagagatgc cgtatttagg gcatagcttc gtcatcgcca taagcccgct
      241 ctccttatca atatcgcgca atttgacaaa ctcctgataa aacctatggt ttctcaactg
      301 tattaacgcc ttaccgcacc gtgggacata caattcctcg ccaccatata cctgcaacag
      361 ctcatgtgtt ttcacttcgc cgatggcttc gaccaaaatt gccaaacgct cggtgtccac
      421 cttgccctta ccaaatttaa accgcgcccc gccaatcgcc ttgaccagct gttccgtcgc
      481 tgccagtccg atgacatcca caatgtccaa cacggtatcc ggcaataaat gttcaacttt
      541 ttcgaacgcc atcatcccca cccgcttttt ccgttttcct gttttccgca atctgcaacg
      601 cagcaaccag tttatgtagc tgcgtatcgt ctaaatattc gaccttatcc ttaccaaaca
      661 tccgccgcgc cattgcgtgt gcatagttcc aatgtttgcc gccgacggtc agcagggctt
      721 cgactttgtc caacattgcc gctgatgatg tccgacgcag atgcggtttg ccgtgtgggt
      781 taccttttgc tttaggctta aatccgtgcg accgcatatc agcgacaaca gactcaagtt
      841 cggaaacatc catatccgca cacgaccgct tgcccgtcac acgctccaac accgcgcgat
      901 aggtaccgtc gtccaagccc agctcctttt gagcaatctt aattttcgca atcaacgccc
      961 ggcgcatctc aaacccataa aacacaatat attgactaat taacgcatat tataccaata
     1021 aaatacaata tatagtatta agccgatgtt ttttgcgaaa cggacagaca taaaaaagca
     1081 actgtatttt tcaccccgtc gggcaaaaat accaaaactc aaatcaagcc gtccggatac
     1141 cgttttcggc ggtatcgttt tcggcaaaat aatcacgcat ccgggcattc gatatcgtca
     1201 gcagtttgcg catacatgcc gtaacggcaa ccttatacgg cttaccctcg gacagcgggc
     1261 gttggtggaa atcccgaata agcggttcaa aacgtgtcgc tgccacggta gccatataca
     1321 gcgccttaag caccgcagac cttccgccaa agcagcggct tttgaatttg gtttccccgc
     1381 tctccctcgg gtgtggggca atgccgacca gactcgctat ccgtttgtgc gacagccgcc
     1441 ccaactcagg caacatcgcc atcagcgtag ccgtcgttat cgaaccgatg cctttgattt
     1501 gctctgccac ttgggctttg ccgtcaaaat gcgtgtgggt gtggttgtcg atttgtttgt
     1561 ccaattcgtc aatcagccgg tcaaaatggg caatcagttg tttgacgctt ccgacttgcg
     1621 tttcatgaac ctgatgcaga cggtttttct cggcagtccg catatccgcc gattggttgc
     1681 ggcggttaac caaggcttcc aacacttctt ccgcttcggt gggcggttgg tagggcatgg
     1741 tttgccaatc ttctttctgc gtcatcatct gtgtgaagaa ggcgggcatt ttggcatctt
     1801 tggcgtcggt tttggtcagc ggctgcgatt gggcaaactg atgcgtctga cgcgggttgg
     1861 cgataatcac ggctatgcct gctcggcg
//
LOCUS       DAWFHY010000157         1721 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.157, whole genome shotgun sequence.
ACCESSION   DAWFHY010000157 DAWFHY010000000
VERSION     DAWFHY010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.157"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(195..1397)
                     /locus_tag="WGQ68_002050"
     CDS             complement(195..1397)
                     /locus_tag="WGQ68_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002254089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEZ0715776.1"
                     /translation="MRKKLTALVLSALPLAAVADVSLYGEIKAGVEGRNFQLQLTEQP
                     QTANTQQGGKVKVTKAKSRIRTKISDFGSFIGFKGSEDLGEGLKAVWQLEQDVSVAGG
                     GATRWGNRESFVGLAGEFGTLRTGRVANQFDDASQAIDPWDSNNDVASQLGIFKRHDD
                     MPVSVRYDSPDFSGFSGSVQFVPIQNSKSAYTPAHWNTVYNTNGTTTTTTTTFVPAVV
                     GKPGSDVYYAGLNYKNGGFAGNYAFKYARHANVGRDAFELFLIGSATSDQAKGTDPLK
                     NHQVHRLTGGYEEGGLNLALAAQLDLSENGDKAKTKNSTTEIAATASYRFGNAVPRIS
                     YAHGFDLIERGKKGENTSYDQIIAGVDYDFSKRTSAIVSGAWLKRNTGIGNYTQINAA
                     SVGLRHKF"
BASE COUNT          446 a          431 c          403 g          441 t
ORIGIN      
        1 tatatgtgca aaccaagccg aaaatgcgaa ataccgtctg aaaatctttc agacggtatt
       61 tgctgtcttt attgccgttt ttcttccgta tccggatttt tgtttggggc tgaagcagat
      121 tggcagtcag attgcaatca aagaatgaaa gcaagccgtc aaaaacaaag ctatccgctt
      181 caccgccccg atatttagaa tttgtggcgc aaaccgacgg aggcggcatt aatttgagtg
      241 tagttgccga tgccggtatt gcgtttcagc caagcgccag acacgatggc ggaagtgcgt
      301 ttggaaaaat cataatcaac gccggcgatg atttgatcgt agctggtatt ttcgcctttt
      361 ttaccgcgtt cgatcaagtc aaaaccatgg gcatagctga tgcgcggaac tgcattaccg
      421 aagcggtagg aagcagtggc ggcaatttcg gtcgtactgt ttttggtttt ggctttgtcg
      481 ccattttcag acaaatccaa ctgagccgcc aaggcgagat tcaagccgcc ttcctcatag
      541 ccgcccgtca ggcggtgtac ctgatggttt ttcaagggat cggtaccttt ggcttgatca
      601 ctcgtcgcgc tgccgattaa gaacaactca aaagcatcac gtccgacatt ggcgtgtctc
      661 gcatatttaa aggcatagtt cccggcaaaa ccgccatttt tgtaattcag accggcataa
      721 tacacatccg atccgggctt gccgacaaca gccggaacga aagtagtagt agtagtagta
      781 gtagtaccgt tagtgttata cacagtattc caatgagccg gcgtataggc ggacttgctg
      841 ttttggatcg gaacgaattg aacgctgccg ctgaaaccgg aaaagtccgg agagtcgtag
      901 cgtacggaaa ccggcatatc gtcgtggcgt ttgaaaatac ccaattgcga agccacatca
      961 ttattgctgt cccaaggatc aatggcttgg ctggcatcgt caaactgatt tgcaacgcgg
     1021 ccggtgcgca gcgtgccgaa ttcgcctgcc aagccgacaa aggattccct attgccccaa
     1081 cgggtcgcgc caccgccggc aacggatacg tcttgctcaa gctgccaaac agctttcaga
     1141 ccttcgccca aatcctcgct ccccttaaag ccgataaacg agccgaaatc actgattttc
     1201 gtcctgatgc ggcttttggc cttagtaact tttaccttac cgccttgctg agtgttagcg
     1261 gtttggggct gctcggtcaa ttgcagctgg aagttcctgc cttccacgcc ggctttgatt
     1321 tcgccgtaca ggctgacatc ggcaacggcc gcaagcggca gtgcggacaa tacgagggcg
     1381 gtaagttttt ttcgcatatc ggcttccttt tgtaaatttg ataaaaacct aaaaacatcg
     1441 ggcaaacacc cgatatgtct tcaattatac cccccccccc gcaaaaaacc atttttcaga
     1501 acaaatatct gataaatgcc gcaaccttta ttttaaaaat gattatattt taatataaaa
     1561 caatagctta ttttttcaaa aaccgttgtg tttctacaac acaattcaag cgcagacctc
     1621 gtgcgggccg atgcgctgct gcccggatgc agtctcggct ttttaaaacg ccataaaaaa
     1681 acacacgcgg cactttatag tggatgaaca aaaaccagta c
//
LOCUS       DAWFHY010000158         1720 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.158, whole genome shotgun sequence.
ACCESSION   DAWFHY010000158 DAWFHY010000000
VERSION     DAWFHY010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1720
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.158"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            342..1646
                     /locus_tag="WGQ68_002051"
     CDS             342..1646
                     /locus_tag="WGQ68_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEZ0715777.1"
                     /translation="MKKHIWAASLLPASLSAEPLNWWKPYSAVNSGDTAWVMTAAALV
                     LLMTLPGLALFYGGMVRKKNLLSTMMHSFSIATLVGILWVAVGYSLAFTPGNAFIGGL
                     GRVFLSGMQIDATAQMLTVSPNAPTVPEPVFMFFQMTFAIISTAIITGAFAERMKYSA
                     MMLFSGIWFLLVYVPGAHWVWGGGFMSKGGVLDYAGGTVVHINAGIAGLVAALVLGRR
                     IGYGREAMPPHNMAMTLIGAAMLWFGWFGFNAGSALAADAAAGMAMAVTQVSAVFGAA
                     GWLACEKIAGHKPSALGLASGAVSGLVGITPAAGFTGPSGAAAIGILTAAACFVSVTV
                     VKHKLRYDDSLDAFGIHGFGGLVGGILTGIFFDNRIFGGDAAVWQQLWIQVKDGFVMA
                     AYSGLMSWAILKVVGKICGGLRVGKDVEREGLDLNIHGERVE"
BASE COUNT          353 a          430 c          509 g          428 t
ORIGIN      
        1 aattaaaaat ttttaatata ttaatcaata aattaatttt ataaaataaa aatattgtca
       61 acaatatttt gccttatcgc ccaaacctct gtatattttc ctacagtaaa ttgttgacaa
      121 tccatacgcc cacatatgcg ccgcctaagg ataaatcctc ccgccggaca acgggtgcaa
      181 gggatcggat gcgatatttc catattcaaa caagggattg gcggcacatc ggcaaaatcc
      241 ccgcgccgcg ccggtccggc agggcttgcg tccctcccgg acaagccccg accccgcctt
      301 tccgaaagac gggctcaacc attaaggaaa ctttaatcaa aatgaaaaaa cacatatggg
      361 cggcatcttt gctgccggca tccctatcgg cagaaccttt aaactggtgg aagccttatt
      421 ccgccgtcaa ttcgggcgat accgcctggg tgatgactgc ggctgccttg gtactgttga
      481 tgacgcttcc cgggctggct ttattctacg gcggtatggt gcggaaaaaa aacctgctct
      541 cgacgatgat gcacagcttt tccatcgcga cattggtggg catcctttgg gtcgccgtcg
      601 gctattcttt agcgttcacg ccgggaaatg cctttatcgg cggtttgggg cgcgtatttt
      661 taagcgggat gcagatagac gctaccgcac agatgctgac cgtgtcgccc aatgcgccga
      721 ctgttcccga accggtattt atgttttttc agatgacgtt tgccattatt tcgaccgcca
      781 ttattaccgg cgcgtttgcc gaacggatga aatattcggc aatgatgctg ttttcgggca
      841 tatggttttt attggtttat gtgccgggcg cgcattgggt gtggggcggc ggctttatga
      901 gcaagggcgg cgtattggat tatgccggcg gtacggtggt gcacatcaat gccggtatcg
      961 cgggactcgt cgccgccttg gttttgggca ggcgcatagg ctacgggcgc gaggcgatgc
     1021 ctccgcacaa tatggcgatg acactgatcg gcgcggcaat gttgtggttc ggctggttcg
     1081 gctttaacgc cggatcggcg cttgcggcag acgcggcggc gggtatggcg atggcggtaa
     1141 cgcaggtgtc ggccgtattc ggcgcggcag gctggcttgc ctgcgaaaaa atagcgggac
     1201 acaaaccttc cgctttgggg ctggcttccg gcgcggtttc cggtctggtc ggcatcaccc
     1261 ctgccgccgg ctttaccggc ccgtcgggcg cggccgccat cggtatattg actgccgccg
     1321 cgtgctttgt gtccgtcacc gtcgtcaaac acaaattgcg ttacgatgat tctttggacg
     1381 ctttcggcat acacggattc ggcgggctgg tgggcggaat attgaccggc atctttttcg
     1441 acaaccgcat tttcggcggg gatgcggcag tttggcagca gttgtggata caggtaaaag
     1501 acgggttcgt tatggcggca tacagcgggc tgatgagttg ggcgattttg aaggtcgtgg
     1561 ggaaaatctg cggcggcctg cgcgtcggca aggatgtcga acgcgaaggt ttggatctga
     1621 atatccacgg cgaacgcgtg gaataagggc ggctatgccg tctgaagcct gaaaatcggg
     1681 tttcagacgg catttttcac gtttgccgcc gatggataaa
//
LOCUS       DAWFHY010000159         1703 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.159, whole genome shotgun sequence.
ACCESSION   DAWFHY010000159 DAWFHY010000000
VERSION     DAWFHY010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.159"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..798
                     /locus_tag="WGQ68_002052"
     CDS             <1..798
                     /locus_tag="WGQ68_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002238292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
                     /protein_id="HEZ0715778.1"
                     /translation="DGAIGAAVGEILGETLLDGRDPGSLNVKDRAKIIAKAKLAAGAV
                     AALSKGDVSTAANAAAVAVENNSLNDIQDRLLSGNYALCMSAGGAESFCESYRPLGLP
                     HFVSVSGEMKLPNKFGNRMVNGKLIINTRNGNVYFSVGKIWSTVKSTKSNISGVSVGW
                     VLNVSPNDYLKEASMNDFRNSNQNKAYAEMISQTLVGESVGGSLCLTRACFSVSSTIS
                     KSKSPFKDSKIIGEIGLGSGVAAGVEKTIYIGNIKDIDKFISANIKK"
     gene            807..1229
                     /locus_tag="WGQ68_002053"
     CDS             807..1229
                     /locus_tag="WGQ68_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715779.1"
                     /translation="MKYMISFLKKTFELMSWVLVILIIGTFYDYYQIRQYAELEKKSI
                     SNILLYAQKEKFRLESKDKYMRGGYTKYKFIFSEYSNTTFLNFINDLKKDNYLPLDGY
                     GHGFLCRKGESISINIYPEINKFILVWGYPENLCADSN"
     gene            1266..1667
                     /locus_tag="WGQ68_002054"
     CDS             1266..1667
                     /locus_tag="WGQ68_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016687567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HEZ0715780.1"
                     /translation="MRKLNNHDVHKRYQDRLEEDVEFTINYELPLSCLWSTIKDFSSD
                     FEEKTEAFFILFKELLRRGHLKLQRDGQIIGHTPEEWEQIFREVWPEYEIEPNPLPGY
                     APFDIGMWLTVEAPAYAVWIDPEDGSEYWAG"
BASE COUNT          581 a          245 c          383 g          494 t
ORIGIN      
        1 gatggtgcga tcggtgcggc ggtcggtgaa atccttggcg aaaccctact ggacggcaga
       61 gaccctggca gcctgaatgt gaaggacagg gcaaaaatca ttgctaaggc gaagctggca
      121 gcaggggcgg ttgcggcgtt gagtaagggg gatgtgagta cggcggcgaa tgcggctgct
      181 gtggcggtag agaataattc tttaaatgat atacaggatc gtttgttgag tggaaattat
      241 gctttatgta tgagtgcagg aggagcagaa agcttttgtg agtcttatcg accactgggc
      301 ttgccacact ttgtaagtgt ttcaggagaa atgaaattac ctaataaatt cgggaatcgt
      361 atggttaatg gaaaattaat tattaacact agaaatggca atgtatattt ctctgtaggt
      421 aaaatatgga gtactgtaaa atcaacaaaa tcaaatataa gtggggtatc tgtcggttgg
      481 gttttaaatg tttcccctaa tgattattta aaagaagcat ctatgaatga tttcagaaat
      541 agtaatcaaa ataaagccta tgcagaaatg atttcccaga ctttggtagg tgagagtgtt
      601 ggtggtagtc tttgtctgac aagagcctgc ttttcggtaa gttcaacaat atctaaatct
      661 aaatctcctt ttaaagattc aaaaattatt ggggaaatcg gtttgggaag tggtgttgct
      721 gcaggagtag aaaaaacaat atacataggt aacataaaag atattgataa atttattagt
      781 gcaaacataa aaaaatagga gttagtatga aatatatgat tagttttcta aaaaaaacat
      841 ttgaattaat gagttgggtg ttagtcatac taataattgg gacattttat gactattatc
      901 aaataaggca atatgctgaa ttagaaaaga aatctatatc aaatatcttg ctatatgccc
      961 aaaaagaaaa atttcgctta gagagtaaag ataaatacat gcgaggagga tatacaaaat
     1021 ataaatttat tttttcagaa tatagtaata ctactttttt aaatttcata aatgacctga
     1081 aaaaagataa ttatttacca cttgacggct atggacatgg ttttctatgt agaaaaggag
     1141 agtctatatc aatcaatata taccctgaaa ttaataaatt tattttagta tggggatacc
     1201 ctgaaaatct ttgcgctgat tctaattaag taagcaagaa taggttatag ggaaataaaa
     1261 ttcaaatgag aaaattgaat aatcacgatg ttcataaacg gtatcaagat cgtttagaag
     1321 aggatgtaga gttcactatc aactatgagc ttcctttgag ttgtttgtgg tcaaccatca
     1381 aagacttttc cagcgatttt gaggaaaaaa ctgaagcgtt ctttattctt ttcaaagagc
     1441 tgctgcgcag aggtcatctg aaactgcaac gcgacgggca aattatcggg catacgcccg
     1501 aagaatggga acaaatattt agggaagtat ggcctgaata tgaaatcgaa cccaatccac
     1561 ttcccggcta tgccccattt gatattggaa tgtggcttac ggtcgaggct cctgcctacg
     1621 ccgtatggat agatcccgaa gacggtagcg aatactgggc gggataaaat accaatgttt
     1681 ggaataaatc ccgtctgaaa aac
//
LOCUS       DAWFHY010000160         1662 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.160, whole genome shotgun sequence.
ACCESSION   DAWFHY010000160 DAWFHY010000000
VERSION     DAWFHY010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.160"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..400)
                     /locus_tag="WGQ68_002055"
     CDS             complement(<1..400)
                     /locus_tag="WGQ68_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595 family transposase"
                     /protein_id="HEZ0715781.1"
                     /translation="MRKSRLSQYKQNKLIELFVAGVTARTAAELVGVNKNTAAYYFHR
                     LRLLIYQNSPHLEMFDGEVEADESYFGGQRKGKRGRGAAGKVAVFGLLKRNGKVYTVT
                     VPNTQTATLFPIIREQVKPDSIVYTDCYRSY"
     gene            707..955
                     /locus_tag="WGQ68_002056"
     CDS             707..955
                     /locus_tag="WGQ68_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003687885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715782.1"
                     /translation="MNIKNEIVALISKKIDGGQYSSLENFALSVLREKIIKNTVSSDE
                     KLLIINDFLSGFLEFDPETGKPVGETLKIEQMIDFFLV"
BASE COUNT          511 a          264 c          337 g          550 t
ORIGIN      
        1 catagctacg ataacaatcc gtataaacaa tgctgtcagg tttcacttgt tcacggataa
       61 taggaaataa agtagcggtt tgagtattcg gtactgtaac cgtataaacc ttaccatttc
      121 gcttcaaaag accgaatacg gcgactttac cggcagcacc gcgaccacgt ttgcctttgc
      181 gttgtccgcc aaaataactt tcatctgctt ctacttcgcc atcaaacatt tctaaatgtg
      241 ggctgttttg ataaataagt aatcgtaaac gatgaaaata ataggctgcg gtatttttat
      301 taacgcctac taactctgct gccgttcttg cagttacacc tgcgacaaat agctcaatga
      361 gtttattttg tttatactgg cttagacgac tttttctcat agggataatt ctaacttaat
      421 ttgaatttcc ctagttatct aggacagccc caaagtttat tgtctttaaa tgagataaca
      481 gataaaacct tatttaaaga aaatgtagtt tatcgtttca gtaagtatta ggtgtgattt
      541 ttattaagtt atttgaggtt ttcggtaagt gtagatataa ggttgagtta taaccaatca
      601 ttcaggctct gttaggcttg gcaactcaac ctgtgcttgc tgaaattggg tatacagtta
      661 tacgttttaa gcgggcttcg ctccctttat catttaaggg taatttatga atattaaaaa
      721 tgaaatagtt gctttaattt ctaaaaaaat tgatgggggg caatatagtt ctttagaaaa
      781 ttttgcttta tccgtactaa gggaaaaaat tataaaaaat actgtttcca gtgatgaaaa
      841 attattgatt attaatgatt ttttatcagg ttttcttgag tttgatcctg agaccgggaa
      901 acctgtcggg gagacgctta aaattgagca aatgattgat ttctttttag tctaaatcat
      961 acataccagt gaacaagcta ggcttatatc cggcttttaa atacgattgt caggtttata
     1021 cttgggataa aatcaaaaat aaagttgttt aaactctagt agattaacaa aaatcaggac
     1081 aaggcgacga agccgcagac agtacagata gtacggaacc gattcactcg gtgcttcaac
     1141 accttagaga atcgttctct ttgagctaag gcgaagcaac gccgtactgg tttttgttaa
     1201 ttcactatat tgcatggtgc agcattaaat cagtcttcta gcgccgcaat gggaaaacat
     1261 agaagatgaa cgaaatggat gatctaatac tatatttttt atcaggtata ttcggaaatc
     1321 aagttgctga atgtattata aaaaacaacc gtgagataaa agttcccttt attgtgcttt
     1381 acgcaatatt ttttacgttg atttatactg tggctttgtt gtttctcagt ctgatctatt
     1441 gggtcaatgg tgcggagatt gcatggaagg ggataggtat tttcagtatg tcggtcagtt
     1501 tttgtatagt cttctgtctt tatttgattg acaaggcagg aagatgtaag gataagaaac
     1561 aatagggatc agatgcggca gcaaacgtag attgggtcta ggcttaacct gcgtttgctg
     1621 catctttgga agaaatagtg gattcgaaaa aataggaaga at
//
LOCUS       DAWFHY010000161         1584 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.161, whole genome shotgun sequence.
ACCESSION   DAWFHY010000161 DAWFHY010000000
VERSION     DAWFHY010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.161"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..136
                     /locus_tag="WGQ68_002057"
     CDS             <1..136
                     /locus_tag="WGQ68_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ0715783.1"
                     /translation="DEYQDIFDFMKQYDLSYPYEYLQDWIDYYTFKTDKLVFGNAKRE
                     "
     gene            complement(718..>1584)
                     /locus_tag="WGQ68_002058"
     CDS             complement(718..>1584)
                     /locus_tag="WGQ68_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0715784.1"
                     /translation="EHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFC
                     RFDELSIPDYSTLCRYRNWLAQDDTLSELLELINRQLTEKGLKVEKASAAVVDATIIQ
                     TAGSKQRQAIEVDNKGQVSGQTTPSKDSDARWIKKNGLYKLGYKQHTRTDAEGYIEKL
                     HITPANAHECKHLPPLLEGIAEGTTVYADKGYDSAENRQHLKEHQLLDGIMRKACRNR
                     PLTETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLL
                     KAANRLSAPAAA"
BASE COUNT          358 a          405 c          394 g          427 t
ORIGIN      
        1 agacgaatat caagacatct tcgattttat gaaacagtat gacttgtctt acccgtatga
       61 atatctgcag gattggatag attactatac gttcaaaacc gataagctgg tatttggtaa
      121 cgcgaagcga gagtgagccg taaaactctg agctcctgtt ttatagatta caactttagg
      181 ccgtcttaaa gctgaaagat tttcgaaagc tataaattga agcccttcca tagtacatag
      241 atctgtgttg tggcggggct ttaccacgct gattgccgga gaagaactca acctgctggc
      301 aaaacaaggc atgagatctt tgcaataaca tgagttgaga cctttgcaaa aaagcccttc
      361 cccgacatcc gaaacccaaa cacaggattt cggctgtttt cgccccaaat acctcctaat
      421 tttacccaaa taccccctta atcctcctcg gacacccgat aatcaggcat ccgggctgcc
      481 ttttaggcgg cagcgggcgc atttagcctg ttggccgctt tcaacaggtt caaacacatc
      541 gccttcaggt ggctttgcgc actcactttg agacctttgc aaaattcctt tccctcccga
      601 cagccgaaac cccaacacag gttttcagct gttttcgccc caaataccgc ctaattttac
      661 ccaaataccc ccttaatcct ccccggatac ccgataatca ggcatccggg ctgcctttta
      721 ggcggcagcg ggcgcactta acctgttggc cgctttcaac aggttcaaac acatcgcctt
      781 caggtggctt tgcgcactca ctttaatcag cccgaaatag gctgcccgcg catagcggaa
      841 tttacggtgc agcgtaccga agctttgttc gaccacataa cgggtcttcg acaaataccg
      901 gttgcgtttg gtttgcgttt ccgtcagcgg acggttgcgg caggctttgc gcataatgcc
      961 gtccaacaac tgatgttctt tcagatgttg ccggttttcc gcactgtcat agcctttatc
     1021 ggcatagacg gtcgtacctt cggctatccc ttccagcaaa ggcggcaggt gtttgcactc
     1081 atgggcattg gcgggggtaa tgtgcagttt ctcgatatag ccttctgcat cggtacgggt
     1141 atgttgtttg taaccgagtt tgtagaggcc gtttttcttg atccaacggg catcgctgtc
     1201 cttactcggt gtggtttggc cgctgacttg tcctttgtta tcgacttcta tagcctgacg
     1261 ctgtttgctg ccggcggtct gaataatggt ggcgtcaacg acagcggcgg atgctttctc
     1321 tacttttaag cctttttcgg tcagttggcg gttaatcagt tccagcaatt cggacagggt
     1381 gtcgtcttgc gccagccagt tgcggtaacg gcataaggtg ctgtaatcgg ggatgctcag
     1441 ttcgtcaaaa cggcaaaaca ggttgaaatc gatgcgggta atgaggctgt gttcgagttc
     1501 gggatcggag agactgtgcc attgtccgag caggacggct ttgaacatgg acaacagggg
     1561 ataggcggga cggccgcggt gctc
//
LOCUS       DAWFHY010000162         1573 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.162, whole genome shotgun sequence.
ACCESSION   DAWFHY010000162 DAWFHY010000000
VERSION     DAWFHY010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.162"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..880
                     /locus_tag="WGQ68_002059"
     CDS             <1..880
                     /locus_tag="WGQ68_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEZ0715785.1"
                     /translation="GGQNNTILFELTGTGCGVAKEGWESRLFAFLTNAIRPKITRVDI
                     AKDFFNGEYSPNQAREDRNKGMFTCHHVKPKGECLGSDWEEDDEAKMTKGKTYGIGSR
                     ESSKYVRVYEKGKQLGDKTSTWTRFEIEFKAKDIVIPFEVLQNPGEYFGGAYPICERF
                     VQKATRIHAVKEDKVISADRYLEWVKKQFGRAANGLKFIFPELDKAKLFELIEPSHHK
                     LPKSLAPEAYDCAFLKAQAIHEQPAFKPYKDPYYMYEYYENLEKQLEQQKHVNNEESY
                     NNFIYDKFARLPISWA"
     gene            925..1215
                     /locus_tag="WGQ68_002060"
     CDS             925..1215
                     /locus_tag="WGQ68_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002250780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715786.1"
                     /translation="MNIQLQGHIVGVKKFNGQIEGKSFDYCRLIVATPLDSSQGNALG
                     SSTTEYDFGGSANFEQFRNAQFPIEANLNVEIVTTGKTQKLKVIGFQLVKKG"
     gene            1220..1417
                     /locus_tag="WGQ68_002061"
     CDS             1220..1417
                     /locus_tag="WGQ68_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715787.1"
                     /translation="MQKVYVVQSVSTGDFLYLSPETGDIGHTKLITNADYFYDFEEAI
                     NAGLEEIGNQYEFVVFGFLKD"
BASE COUNT          500 a          383 c          335 g          355 t
ORIGIN      
        1 tggcggacaa aacaatacca tccttttcga actgaccggc accggttgcg gcgtcgcaaa
       61 agaaggctgg gaatcacgac ttttcgcatt cctgactaat gcaatccgcc caaaaatcac
      121 acgcgttgac atcgcaaaag actttttcaa cggcgaatac agcccgaacc aagcccgtga
      181 agaccgaaat aaaggtatgt ttacctgtca tcacgtcaaa ccaaaaggcg aatgtttggg
      241 gtcagattgg gaagaagacg atgaagccaa aatgaccaaa ggcaagacct atggtatcgg
      301 ctcccgtgaa tcgtccaaat atgtccgcgt ctatgaaaaa ggcaagcagt tgggcgataa
      361 aacaagcaca tggacgcgat ttgaaattga attcaaagca aaagacatcg ttatcccttt
      421 cgaagttttg cagaatccgg gcgaatattt cggcggcgca tatccgattt gcgaacgatt
      481 cgtccaaaag gcaacgcgca tacacgcggt taaggaagat aaggtcattt cagccgaccg
      541 ctaccttgaa tgggtaaaaa aacagttcgg acgtgcggca aacggtctga aattcatttt
      601 tcccgaattg gacaaagcca aactgtttga actgattgag ccgagtcatc acaagctgcc
      661 caagtctttg gctcccgaag cctacgactg cgcctttttg aaagctcaag ccattcatga
      721 acagcccgca ttcaaaccgt acaaagaccc ttactatatg tacgaatatt acgagaatct
      781 tgaaaaacag cttgaacagc aaaaacacgt caacaatgaa gaaagctata acaacttcat
      841 ttacgacaaa ttcgcaagac taccgatttc atgggcttaa agtgtctgcc cgaaagacgt
      901 ttaatcacac aaggaaacca aaaaatgaac atccaacttc aaggccacat cgtcggcgtt
      961 aaaaaattca acggacaaat cgaaggcaag agcttcgact attgccgcct gattgtcgcc
     1021 acacccttag acagctccca aggcaacgca ttgggcagct ctactactga atacgatttc
     1081 ggcggctctg ccaatttcga gcagttccga aacgcccaat ttccgatcga agcaaacctg
     1141 aacgtagaaa tcgttactac gggcaaaacc caaaaactga aagtcatcgg ttttcaactc
     1201 gtgaagaaag gctgattgaa tgcagaaagt ctatgttgtc cagtccgtat caacagggga
     1261 ctttctgtat ctctctcctg aaacgggcga catcggacat accaaattaa tcaccaatgc
     1321 cgattatttc tacgacttcg aagaagcgat taacgcaggt ttggaagaaa tcggcaacca
     1381 atacgaattt gtcgtattcg gatttttgaa agactgattt tcggatgttc ggcggtcgtc
     1441 tgaaaaacgc tccatccatt accgccaaac actttttgaa ggaaaatatc atgaaattta
     1501 ttaacacctg ccgtaaatac ggcgcaaaac tggctgttgt aacagctgct cccctggctt
     1561 tggccgcaca tgc
//
LOCUS       DAWFHY010000163         1546 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.163, whole genome shotgun sequence.
ACCESSION   DAWFHY010000163 DAWFHY010000000
VERSION     DAWFHY010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.163"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..457)
                     /locus_tag="WGQ68_002062"
     CDS             complement(<1..457)
                     /locus_tag="WGQ68_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fhaB protein"
                     /protein_id="HEZ0715788.1"
                     /translation="MNKTLYRVIFNRKRGAVVAVAETTKREGKSCADSDSGSAHVKSV
                     PLGTTHAPVCRSNIFSFSLLGFSLCLAVGTANIAFADGIIADKAAPKTQQATILQTGN
                     GIPQVNIQTPTSAGVSVNQYAQFDVGNRGAILNNSRSNTQTQLGGWIQGN"
     gene            complement(674..1093)
                     /locus_tag="WGQ68_002063"
     CDS             complement(674..1093)
                     /locus_tag="WGQ68_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715789.1"
                     /translation="MKRIKCFCDKFPSGDTFRMCIILDDYDNRVDYYVGIYDYITSTL
                     MSDIYYRSAIDEHFKIIELIENNPNEIYDDGGGQQFCLEFHHDKVIFYHNEFDEEDGY
                     PVLSCSLHTFKTALIAWNAFLQLPKSIHSVVETVIEE"
     gene            complement(1124..1495)
                     /locus_tag="WGQ68_002064"
     CDS             complement(1124..1495)
                     /locus_tag="WGQ68_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003704451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0715790.1"
                     /translation="MRIRRGDINKKGTVTGGHSLTRGDVRVIQQTSAPDKHGVYQATV
                     EIKKPDGSWEVKTKKGGKVMTKHTMFPKDWDEARIRAEVTSAWESRIMLKDNKWQGTS
                     KSGIKIEGFTEPNRTAYPIYE"
BASE COUNT          461 a          333 c          252 g          500 t
ORIGIN      
        1 gattaccttg aatccaaccg cctagctgtg tttgggtgtt gctgcggctg ttgtttaaaa
       61 tcgccccgcg attacccaca tcaaactggg cgtattgatt aacagaaacc cctgccgaag
      121 taggggtttg aatattgact tgcggtatgc cgttacctgt ttgcagaatc gtggcttgtt
      181 gggttttagg agcagcttta tcagcaataa tgccatcagc aaaagcaata ttggccgtac
      241 ctacagccaa acataaagaa aagcccaata aagaaaaaga aaagatattt gaacgacaaa
      301 caggtgcatg agtagtaccc aaaggaacag atttcacatg agcgctgcct gaatcactat
      361 cggcacagct tttaccttcg cgcttggtag tttcagcaac ggctaccaca gccccacgtt
      421 tgcggttgaa aattacacga tagagagttt tattcatgat ttcagttatt tgatttttat
      481 agagttatta aaaaaataga taatcttaat tattctaatt caaggatttt atctaaatca
      541 aatgcttact gccattcgtt tatgagaact tgtattcaat acaagttcta acatcttaat
      601 catggcacag cgtataagcg tggatttgca gtccaatcta tatcgctgat tctattcatt
      661 aagctaatta tgcttattcc tcaatcacag tctccaccac cgaatgaata cttttaggca
      721 attgcaaaaa agcattccaa gcaattaaag cagttttaaa agtatgcagc gaacagctta
      781 atactggata accatcttct tcatcaaatt cattgtggta aaaaatgacc ttatcatgat
      841 gaaattctag gcaaaattgt tgaccaccgc catcatcata aatttcattt ggattatttt
      901 ctattaattc tataatcttg aaatgctcat caatcgcgga tcgatagtaa atatcgctca
      961 ttaaggtaga cgtaatgtaa tcatatattc ctacataata atcaacccta ttatcatagt
     1021 catccagaat gatacacatt ctaaatgtat ctcctgatgg aaatttatca caaaagcact
     1081 taattctttt catcattaat ctcctaattt ttcataaata ttactattca taaatgggat
     1141 atgctgttct attaggttcg gtaaatcctt ctattttaat acccgattta cttgtaccct
     1201 gccatttatt atccttaagc attattctac tttcccaagc cgaagtaact tcagccctaa
     1261 ttctagcctc atcccaatct tttgggaaca tggtgtgctt ggtcatcact ttcccacctt
     1321 ttttcgtttt cacctcccaa cttccatcag gctttttaat ttccactgtc gcttgataaa
     1381 ccccatgttt atcaggtgcc gaggtttgtt gtatcacccg tacatcacca cgggttagac
     1441 tatgccctcc tgttactgtg cctttcttat tgatatcacc acggcggatt cgcatttgaa
     1501 gtgcaacttt ccctaacaga aaaaggccag tatgcggtag catacg
//
LOCUS       DAWFHY010000164         1516 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.164, whole genome shotgun sequence.
ACCESSION   DAWFHY010000164 DAWFHY010000000
VERSION     DAWFHY010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1516
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.164"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(157..1146)
                     /gene="porB"
                     /locus_tag="WGQ68_002065"
     CDS             complement(157..1146)
                     /gene="porB"
                     /locus_tag="WGQ68_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric porin PorB"
                     /protein_id="HEZ0715791.1"
                     /translation="MKKSLIALTLAALPVAAMADVTLYGTIKAGVETSRSLDYQDGQV
                     YSVETGTGIVDLGSKIGFKGQEDLGNGLKAIWQVEQKASIAGTDSGWGNRQSFIGLKG
                     GFGKLRVGRLNSVLKDTGDINPWDSKSDYLGVNKIAEPEARLISVRYDSPEFAGLSGS
                     VQYALNDNAGRHNSESYHAGFNYKNGGFFVQYGGAYKRHQNVDNVKIEKYQIHRLVSG
                     YDNDALYASVAVQQQDAKLVKDNSHNSQTEVAATLAYRFGNVTPRVSYAHGFKGSFDD
                     ADIGNEYDQVVVGAEYDFSKRTSALVSAGWLQEGKGAYKFVSTAGGVGLRHKF"
BASE COUNT          375 a          340 c          394 g          407 t
ORIGIN      
        1 cctgcgcggg aatgacggca tattttgaca ttgaataaaa aagactaaaa acaggaaaaa
       61 gccaaacaga aaacagcgaa acagaaaaca ggaaaaagcc aaacagaaaa aagcctgtct
      121 ggcgacaggc tttttgttga taccaatctt tgcagattag aatttgtggc gcagaccgac
      181 accgccggca gtcgatacga atttgtatgc gcctttgcct tcttgcaacc agccggcaga
      241 aaccaaggca gaagtgcgtt tggagaagtc gtattccgca ccgacaacca cttggtcgta
      301 ctcgttgcct atgtctgcat catcaaacga gcctttgaag ccgtgggcgt aagaaacgcg
      361 gggcgttacg ttgccgaagc ggtatgccaa ggtagcggca acttcggttt gagagttgtg
      421 cgaattgtct ttaaccagtt tcgcgtcttg ttgctgtacg gctacggaag cgtacagggc
      481 atcattgtcg taaccgctga ccaaacggtg aatctggtat ttctcaatct tcacgttatc
      541 cacattctga tgtcttttat aggcaccgcc atattgcacg aagaagccgc cgtttttgta
      601 gttgaagccg gcgtggtaag attcgctgtt atgtctgcct gcattgtcgt taagcgcgta
      661 ttgtacgctg ccgctgaggc cggcaaattc gggagaatcg tagcgtacgg aaatgaggcg
      721 tgcttcgggt tcggcaattt tgtttacacc caaatagtcg cttttgctat cccaaggatt
      781 gatgtcgccg gtgtctttca ggacgctgtt caaacggccg acgcgcaatt taccgaagcc
      841 gcctttcaag ccgatgaagg attggcggtt gccccaaccg gagtcagtac cggcgataga
      901 tgctttttgc tcaacctgcc aaatggcttt caggccgtta ccgaggtctt cttggccttt
      961 gaagccgatt ttcgaaccca aatcaacgat gccggtaccg gtttcaacgc tataaacttg
     1021 accgtcctgg taatctaggg agcgggaagt ttctacgccg gctttgatgg tgccgtacag
     1081 ggtaacgtca gccattgctg caacaggaag ggctgccaaa gtcagggcaa tcagggattt
     1141 tttcattgct gtattccttt tttggttaag aaatttaagc aggccgggct ttccaagccg
     1201 cttagctttg catttaccgc cgacgttttc ggcgattcca tgtctgaaac tatagatgtt
     1261 cctgtctgaa aaaacaaact tctgcctgtt tttgtgtgga attcgatgtg tattttgaag
     1321 ggcggataga gatattgtgg gtattttttg ttttataaca tatggttatt taaatttttt
     1381 aagatttgca tttttacaac acttactcgg gagggtattg gagggcattg caaaccgggg
     1441 gttataaaga cggcaaaaaa cgcgcacgcg gtttctttcg gaacgggcgg atgcaaaacc
     1501 cgcccctgcg gcagga
//
LOCUS       DAWFHY010000165         1457 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.165, whole genome shotgun sequence.
ACCESSION   DAWFHY010000165 DAWFHY010000000
VERSION     DAWFHY010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.165"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(756..1430)
                     /locus_tag="WGQ68_002066"
     CDS             complement(756..1430)
                     /locus_tag="WGQ68_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0715792.1"
                     /translation="MQADLAYAAERITHDYPKATGANNTSTVSDYFRNIRAHSIHPRV
                     SVGYDFGGWRIAADYASYRKWKESNSSTNAENRDSIQNYVKIETKHQGNGSFHAVSSL
                     GLSAIYDFKLNDKFKPYIGARVAYGHVKHQVHSVESKNTIVTSKPTQGAAQGGTVPTG
                     FVPKSAYHESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSL
                     GVRYRF"
BASE COUNT          318 a          315 c          427 g          397 t
ORIGIN      
        1 catccatacg gaaacctgca tcccgtcatt cccacgaaag tgggaatcta gaacgcgggg
       61 tttgggcaac tgtttttatc cgataagttt ctgtgcggac aggtctggat tcccgcctgt
      121 gcgggaatga cgaatttcaa gattgcggtg ttgtcggacg ggttttgaga ttacggtgtt
      181 gtcggagcgc aactgaaccg tcattcccac ggaagtggga atctagaacg cggggtttca
      241 gtcatttccg atagattccc gccgcgtcag ggggctggat tcccgcctgc gcgggaatga
      301 cgaatttcga gattacggtg ttgtcgggaa tgacgggttt caagattgcg ctgttatcgg
      361 gaatgacgaa tttcgagatt acggtgttgt cggaacgcaa ctgaaccgtc attcccacgg
      421 aagtgggaat ctagaatctc ggggtttcag tcatttccga tagattcccg ccgcgtcagg
      481 ggtctggatt cccgcctgcg caggaatgac gggtttcgag attgcgttgt tgtcgggaat
      541 gcaactgaac cgtcattccc acggaagtgg gaatctagga cgtaaaatct aaagaaaccg
      601 ttttatccga taagtttctg tgcggacagg tctggattcc cgcctgcgcg ggaatgacga
      661 atttcgagat tacggtgtat cgggaatgat gggaaacggt gggaattgtg taaaaaatgc
      721 cgtctgaagg ttcagacggc atcggtgtcg gggaatcaga agcggtagcg cacgcccaat
      781 gagacttcgt gggttttgaa gcgggtgttt tccaagcgtc cccagttgtg gtagcggtat
      841 ccggtgtcca gggtcagctt gggcgtgatg tcgaaaccaa caccggcgat gacaccaaga
      901 cccaagctgc tgatgctgtg gctttcgtga taggcagatt tggggacaaa acctgttggg
      961 acagtacctc cctgtgcagc gccttgcgtt ggtttagagg taacaatcgt gtttttgctt
     1021 tccactgaat gaacctgatg tttaacgtgt ccgtaggcga cgcgcgcgcc gatatagggt
     1081 ttgaatttat cgttgagttt gaaatcgtaa atggcggata agccgagaga agaaacggcg
     1141 tggaagctgc cgtttccttg atgttttgtt tcaatcttta cgtagttttg tatactatct
     1201 ctattttctg cattagtaga agaattactt tctttccatt ttctgtaact ggcataatct
     1261 gccgctattc tccagccgcc gaagtcgtag ccgactgaca ctcgggggtg gatggaatgc
     1321 gcacggatgt ttctgaaata atcgcttact gtgcttgtgt tgtttgcacc ggttgctttc
     1381 ggataatcgt gggtaatgcg ttcggcggca taagctaaat ccgcctgcac ataatacggg
     1441 ctgcggctgc cgtcttc
//
LOCUS       DAWFHY010000166         1388 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.166, whole genome shotgun sequence.
ACCESSION   DAWFHY010000166 DAWFHY010000000
VERSION     DAWFHY010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.166"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            34..858
                     /locus_tag="WGQ68_002067"
     CDS             34..858
                     /locus_tag="WGQ68_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0715793.1"
                     /translation="MQPPKNFLFSSLLFSSLLFSSLLFSSAAQAASEDGSRSPYYVQA
                     DLAYAAERITHDYPKPTGTDKDKISTVSDYFRNIRAHSIHPRVSVGYDFGDWRIAADY
                     ASYRKWNNNKYSVNTKEVQRNNNTGTDIWKELKTENQENGSFHAVSSLGLSAIYDFKL
                     NDKFDKFKPYIGARVAYGHVKHQVHSVRKETTTVFTKSKGITEPGKVVQGPVSQPAYH
                     ESHSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGMRYHF"
BASE COUNT          379 a          407 c          302 g          300 t
ORIGIN      
        1 atcagttaaa accgaatacg gagtcgaaaa tgaatccagc ccccaaaaaa ctttctcttc
       61 tcttctcttc tcttctcttc tcttctcttc tcttctcttc tcttctcttc cgcagcgcag
      121 gcggcaagtg aagacggcag ccgcagcccg tattatgtgc aggcggattt agcctacgcc
      181 gccgaacgca ttacccacga ttatccgaaa ccaaccggta cagacaaaga caaaataagc
      241 acggtaagcg attatttcag aaacatccgt gcgcattcca tccacccccg ggtgtcggtc
      301 ggctacgatt tcggcgactg gagaatagcg gcagattatg ccagttacag aaaatggaac
      361 aacaataaat attccgtcaa cacaaaagag gtgcaaagaa acaataacac tggcactgac
      421 atctggaaag aactgaagac ggaaaatcag gaaaacggca gcttccacgc cgtctcttct
      481 ctcggcttat ccgccattta cgatttcaaa ctcaacgata aattcgataa attcaaaccc
      541 tatatcggcg cgcgcgtcgc ctacggacac gttaaacatc aggttcattc agtgagaaaa
      601 gaaaccacga ctgttttcac taaatcaaaa ggcattacag agccaggcaa ggtcgtacaa
      661 ggtccagtca gccaacctgc ctatcacgaa agccacagca tcagcagctt gggtcttggt
      721 gtcatcgccg gtgtcggttt cgacatcaca cccaagctga ccttggacac cggataccgt
      781 taccacaact ggggacgctt ggaaaacacc cgcttcaaaa cccacgaagt ctcattgggc
      841 atgcgctacc acttctgatt ccccgacacc gatgccgtct gaaccttcag acggcatttt
      901 tgattcacct gccgtttaca ggcgcggggc gggcgtggaa atacccgaac cgtcattccc
      961 gacaacaccg taatcttgaa acccgccatt cccgacaata ccgcaatctc gaaattcgtc
     1021 attcccgata ataccgcaat ctcgaaattc gtcattcccg cgcaggcggg aatccagact
     1081 ccctgacgcg gcgggaatct atcggaaatg actgaaaccc cgggattcta gattcccact
     1141 ttcgtgggaa tgacgtggtg caggtttccg tatggatgga ttcgtcattc ccgacaatac
     1201 cgcaatcttg aaacccatca ttcccgcgca ggcgggaatc tagaccccct gacgcggcgg
     1261 gaatctatcg gaaatgactg aacccccgag attctagatt cccactgtcg tgggaatgac
     1321 ggttcagttg cgttccgaca acaccgcaat ctcgaaaccc gtcattcccg cgcaggcggg
     1381 aatccaga
//
LOCUS       DAWFHY010000167         1382 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.167, whole genome shotgun sequence.
ACCESSION   DAWFHY010000167 DAWFHY010000000
VERSION     DAWFHY010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.167"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..356)
                     /locus_tag="WGQ68_002068"
     CDS             complement(<1..356)
                     /locus_tag="WGQ68_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0715794.1"
                     /translation="MVKTADGYKAIAHIQTGEHVFAKDETSGKTGYKPVTTRYGNPYQ
                     ETVYIEVSDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSESGKTQTVRNIVV
                     KPKPLKAYNLTVADWHT"
     gene            complement(571..930)
                     /locus_tag="WGQ68_002069"
     CDS             complement(571..930)
                     /locus_tag="WGQ68_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003693721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715795.1"
                     /translation="MASYVSIKGWIECSDDDIKIIQENINNFWNNCPFNIEEKESAKI
                     YKSGWVFPTNSFNWSSYIFFGACVKSYFIIYFEKCIKTIMELDIEISGFFELDYYEDN
                     YKVNWKINNGNLIQTTN"
     gene            complement(933..1289)
                     /locus_tag="WGQ68_002070"
     CDS             complement(933..1289)
                     /locus_tag="WGQ68_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003697371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715796.1"
                     /translation="MSDSARQLYQNAKYREALDIHYEDLIRRKTDGSSKFINGREIDA
                     VTNDALIQAKRTISAIDKPKNFLNQKNRKQIKATIEAANQQGKRAEFWFKYGVHSQVK
                     SYIESKGGIVKTGLGD"
BASE COUNT          359 a          269 c          243 g          511 t
ORIGIN      
        1 gtatgccaat cggcaacggt cagattgtag gctttgagcg gttttggttt gacaacgatg
       61 ttgcggacgg tttgggtttt gccgctttcg gataacagcc ggcttcccgc tttcaaatct
      121 tccgctttaa tccatttccc atccgaataa aacggatgga tgcggttgga aatcagggtt
      181 tggctgttgc cgatgccgtc tgaaacttca atgtaaacgg tttcctggta cggattgccg
      241 tatcgggtgg taacgggttt gtatccagtt tttccactgg tttcatcttt ggcaaagacg
      301 tgctctccag tttggatatg tgcgatggct ttgtagccgt ctgccgtttt gaccaaggtg
      361 ctgccgtgga aggagcaggt gtaggatttt taaagaactt agttctcaat atcctgtttc
      421 attcatcaaa atgccgtctg aaagctgaat accgcttcag acggcatttt ggtggttggg
      481 tttttaagcc aacctacgct tactgaaaac caaattgagt ttcagacagt ttttaggttt
      541 gggtgtccaa tctaacttat atttgtccat ttaattagtc gtttgtatca aatttccatt
      601 atttattttc caatttactt tataattatc ttcataataa tctaattcaa aaaaacctga
      661 tatttcaata tccaattcca ttattgtttt aatacatttt tcaaaataaa taatgaaata
      721 agattttacg catgcaccaa aaaaaatata gctgctccaa ttaaaactat ttgtcgggaa
      781 aacccacccg cttttatata tttttgcaga ttctttctct tcgatattaa agggacaatt
      841 attccaaaaa ttattaatgt tttcttggat gatttttata tcatcgtcag agcattcaat
      901 ccatcctttt atggaaacat atgatgccat gtttaatctc ctaaacctgt tttaacaatg
      961 ccgccttttg attcaatata tgacttaact tgtgaatgaa caccgtattt aaaccaaaat
     1021 tctgcacgtt ttccctgttg gtttgctgct tcgatggttg ctttaatttg ctttctattt
     1081 ttttgattta agaaattttt aggtttatct attgctgaaa ttgttctttt ggcttgtatt
     1141 aaagcatcat tcgtaacagc gtcaatttct ctgccgttaa taaattttga tgaaccatca
     1201 gtttttcttc taattaaatc ttcataatgt atatctagag cttctctata ctttgcattt
     1261 tgatataact gtctcgcact atcagacaaa gccaatttct ttttataaga atcagcaaaa
     1321 tccccgctaa ccgcagcctt ccctggtttt gccgcctttg ccaacttcgc gactttggct
     1381 gc
//
LOCUS       DAWFHY010000168         1357 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.168, whole genome shotgun sequence.
ACCESSION   DAWFHY010000168 DAWFHY010000000
VERSION     DAWFHY010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1357
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.168"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..380)
                     /locus_tag="WGQ68_002071"
     CDS             complement(<1..380)
                     /locus_tag="WGQ68_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003690617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715797.1"
                     /translation="MSFGYLIATSQPCELLTKSRGETFSLIMDKMDLWIYFRFCEGNI
                     YTIRKNETESCLTERGGKWLKHIYEFNRGSFIFSYVLLKKRESEENFAEIVLKSIKNN
                     KILTVKVRSGLHFDLRNIYRIEMY"
     gene            complement(438..803)
                     /locus_tag="WGQ68_002072"
     CDS             complement(438..803)
                     /locus_tag="WGQ68_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715798.1"
                     /translation="MEKPLLTPPFFLFEDVSLDIFQGLSELEKKIEPQDLMDDVYRAF
                     DSVGNILNFRIVEKEQKGFWVSTKIKTVVFDSADMSSDDLFLKCLQSSYKAYFETEPA
                     GLDKRQLMKTLIQKCGFSC"
     gene            complement(785..1330)
                     /locus_tag="WGQ68_002073"
     CDS             complement(785..1330)
                     /locus_tag="WGQ68_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715799.1"
                     /translation="MKKQITAAVMMLSMIAPAMANSMDNQIFENQVFHTRADAPMQLA
                     ELSQKEMKETERAFLPLAILGGAAIGMWTQHGFSYATTGRPASVRDVAIAGGLGAIPG
                     GVGAAGKVVSFAKYGREIKIGNNMRIAPFGNRTGHPIGKFPHYHRRVTDNTGKTLPGQ
                     GIGRHRPWESKSTDRSWKNRF"
BASE COUNT          414 a          309 c          209 g          425 t
ORIGIN      
        1 ctatacatct caatacgata aatatttctt aagtcaaaat gcaagcctga ccgtacctta
       61 actgtcaaaa ttttattatt ttttattgat tttaaaacaa tttctgcaaa attctcttcg
      121 ctttctctct tttttagaag cacataagaa aaaataaaac ttccccgatt aaattcataa
      181 atatgtttca accatttgcc tcctctttct gtaagacaag attcagtttc attcttcctt
      241 attgtataaa tatttccttc acaaaatctg aaataaatcc ataaatccat cttatccata
      301 attaaagaaa aagtttcacc tcgagatttt gtcaacaatt cgcaaggttg cgatgttgca
      361 atcaaatagc cgaaagacat tttttacctc atacatggtc gaaatcagtt tctgttagtt
      421 cagaatccat tttttcgtca acaactgaat ccgcattttt gaattaacgt tttcatcagc
      481 tgccgtttat ctaaaccggc aggttcagtt tcaaaataag ccttatatga agactgtaag
      541 catttcagaa aaagatcatc agaagacata tctgccgaat caaatacaac tgttttgatt
      601 ttggtactta cccaaaaccc tttttgctct ttttctacta tacggaaatt cagaatattt
      661 ccaaccgaat caaaagcacg gtaaacatca tccatcaaat cctgcggctc tattttcttt
      721 tccaattccg acaatccttg aaatatatcc aaagacacat cttcaaatag aaaaaaagga
      781 ggagttagaa gcggtttttc catgatctgt ccgtagattt tgattcccaa gggcgatgac
      841 gaccaattcc ctgtccaggc aaagtcttgc ccgtattatc cgtaactcga cgatgataat
      901 ggggaaattt tccaatagga tgacctgttc tattaccgaa aggggctatc cgcatattat
      961 tgccgatttt aatctcacgt ccatatttag caaaggaaac aacctttcct gcggcgccta
     1021 caccaccagg aattgcgcct aatccgccag caatagcaac atctctaaca gaagctggtc
     1081 tgcctgtcgt tgcataacta aaaccatgct gtgtccacat accaatggca gcaccaccca
     1141 agatagccaa tggaagaaac gccctctcag tctccttcat ctccttttga gaaagttccg
     1201 ccaactgcat cggcgcatct gcccgcgtat ggaatacttg gttttcaaat atctgattgt
     1261 ccatgctgtt tgccattgcg ggggcaatca tagacagcat cattacggct gcggtaattt
     1321 gttttttcat aataactcct ttggattaca aggttgg
//
LOCUS       DAWFHY010000169         1322 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.169, whole genome shotgun sequence.
ACCESSION   DAWFHY010000169 DAWFHY010000000
VERSION     DAWFHY010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1322
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.169"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(113..1036)
                     /locus_tag="WGQ68_002074"
     CDS             complement(113..1036)
                     /locus_tag="WGQ68_002074"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HEZ0715800.1"
                     /translation="MLQRTLAKSISVTGVGLHSGERVALTLHPAPENSGISFRRTDLD
                     GEMGEQIKLTPYLINDTRLSSTIVTDKGVRVGTIEHIMSALSAYGIDNALIELNAPEI
                     PIMDGSSLPFIYLLQDAGVVDQKAQKRFLKILKPVEIKEAGKWVRFTPYDGFKVTLTI
                     EFDHPVFNRSSPTFEIDFAGKSYIDEIARARTFGFMHEVEMMRAHNLGLGGNLNNAIV
                     IDDTDVLNPEGLRYPDEFVRHKILDAIGDLYIVGHSIVGAFEGYKSGHAINNALLRAV
                     LADETAYDRVEFADSDDLPDAFHELNIRNCG"
BASE COUNT          350 a          317 c          332 g          323 t
ORIGIN      
        1 gattcccact ttcgtgggaa tgacgaattt taggttgctg ttttggtttt ttgtccttgt
       61 gggaatgatg aaaatttaag ttttaggaat ttaccggaaa aaacagaaag cgttatccac
      121 aatttctgat gttcagctcg tgaaatgcgt cgggcaaatc atcgctgtcg gcaaattcca
      181 cccggtcgta agccgtttcg tctgccaaaa ccgcgcgcaa gagtgcgttg ttgatggcgt
      241 gtcccgattt gtagccttca aacgcgccga caattgaatg tccgacgata tacaaatcac
      301 cgatggcatc aaggattttg tggcgcacaa actcatcggg atagcgcaag ccttcaggat
      361 tcaggacatc cgtgtcgtca atcacgatgg cgttgttcaa attgccgccc aaacccagat
      421 tgtgggcgcg catcatttcc acttcgtgca taaagccgaa agtgcgcgca cgcgcgattt
      481 cgtcgatgta ggatttgccg gcgaaatcga tttcaaaagt gggcgagctg cggttgaaaa
      541 ccggatggtc gaattcgatg gtcagcgtta ccttaaagcc gtcatacggc gtaaagcgca
      601 cccatttgcc cgcttctttg atttcgacag gtttgaggat tttcaaaaaa cgcttttgcg
      661 ccttttgatc gaccacgccc gcatcttgca aaaggtaaat aaacggcagg ctggagccgt
      721 ccataatcgg gatttcgggc gcgttcagct caatcagcgc gttgtcgata ccgtaggcgg
      781 acagcgcgga cataatgtgt tcgattgtgc cgacgcgcac gcctttgtcg gtaacgatgg
      841 tggaggaaag gcgggtatcg ttgatcaaat aaggggtcag tttgatttgt tcgcccatct
      901 cgccgtccaa atcggtacgg cggaaggaaa tcccgctgtt ttcaggcgcg gggtgcaggg
      961 tcagcgcgac gcgttcgccc gaatgcagcc cgactccggt aacgctgatg gatttcgcca
     1021 aagttcgttg cagcataaac cgcttcctta tcaatggggt aagttttgga ataatacgat
     1081 aaaaccggaa aaacaggcta tgtttttcca tagtatttgc caatgtatcc gttttcaata
     1141 cgtaagccgc ataaaaatgt atagtggatt aacaaaaatc aagacaaggc gacgaacccg
     1201 cccccctcct gaaaaacgca aaaaatgccg tccgaaaacc tttcggacgg cattttcgcg
     1261 tgaaccgtca ttcccacaag gacataaaac caaaacagaa aaccaaaaac agaaacctac
     1321 at
//
LOCUS       DAWFHY010000170         1296 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.170, whole genome shotgun sequence.
ACCESSION   DAWFHY010000170 DAWFHY010000000
VERSION     DAWFHY010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.170"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            1..109
                     /locus_tag="WGQ68_002075"
                     /pseudo
     CDS             1..109
                     /locus_tag="WGQ68_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
     gene            1160..>1296
                     /gene="pilE"
                     /locus_tag="WGQ68_002076"
     CDS             1160..>1296
                     /gene="pilE"
                     /locus_tag="WGQ68_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial major subunit PilE"
                     /protein_id="HEZ0715801.1"
                     /translation="MNTLQKGFTLIELMIVIAIVGILAAVALPAYQDYTARAQVSEAI
                     L"
BASE COUNT          425 a          342 c          225 g          304 t
ORIGIN      
        1 cggttcggta aaatggttct gcggacagcc ggttacgcgc gacgacgcca aagccaccaa
       61 cgacgacgtt accgccgaca acggcaccga caagaaaatc gacaccaaac tagagaaacc
      121 tttttacccg ataagtttcc gcaccgacga ggctggattc ccgcctgcgc gggaatgacg
      181 gcggagcggt ttctgttttt tcgataaatt cctaaaactt aaaatttcat cattcccaca
      241 aaaacagaaa accaaaaaca gcaacctaaa attcgtcatt cccgcaaaag cgggaatcta
      301 gttcgttgag tttcagttat ttagaataaa ttttgaaact ttaatcccgt cattcccacg
      361 aaagtgggaa tccaggacgc aaaatctcaa gaaaccgttt tcccgataag tttctgcacc
      421 gacaaggctg gattcccgcc tgcgcgggaa tgacggcgga gcggtttctg ttttttccga
      481 taaattccta aaacttaaat tttcatcatt cccacaaaaa cagaaaacca aaaacagaaa
      541 cctaaaattc gtcattccca cgaaagtggg aatctagaat ctcggacttt cagataatct
      601 ttgaatattg ctgttgttct aaggtctaga ttcccgcctg cgcgggaatg acgaagccat
      661 ccgcacggaa acctgccgcg acgattcaac tgccgtttgc acaggaacac cacgcgccga
      721 tttcaatcac tttccaagaa aacggaaatt tttaaaaatt aaatttaaaa tttcccaccc
      781 aacccaccct atgctaccgc gcaaattcaa aaatctcaaa ttccgaccca atcaacacac
      841 ccgatgcccc atgccaatag agatacccca cgccaataaa aaagtgccgc aaatcggcac
      901 taaaactgac aattttcaac actactaaca aaaaacggta accccctgct tcctcaaacc
      961 acgcccaaac gaaagaaaat gtaaaataaa aacaaccata taaagataaa cgcataaatt
     1021 ttcacctcaa aacacaaact tgataccaat cttgctttat aatacacagg tatcgcaaca
     1081 aaataccgat ggtcaaatac attgcataat gccgatggcg taagcttgag gcatttcctt
     1141 tccaattagg agtaatttta tgaacaccct tcaaaaaggt tttaccctta tcgagctgat
     1201 gattgtgatt gccatcgtcg gcattttggc ggcagtcgcc cttcctgctt atcaagacta
     1261 cacagcccgc gcgcaagttt ccgaagccat tctttt
//
LOCUS       DAWFHY010000171         1292 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.171, whole genome shotgun sequence.
ACCESSION   DAWFHY010000171 DAWFHY010000000
VERSION     DAWFHY010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.171"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..543
                     /gene="mafB"
                     /locus_tag="WGQ68_002077"
     CDS             <1..543
                     /gene="mafB"
                     /locus_tag="WGQ68_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin MafB class 1"
                     /protein_id="HEZ0715802.1"
                     /translation="AAKVAKLAKAAKPGKAAVSGDFSAAYNTRTTRKVTTETEGLNRI
                     RQNQKNSNIHEKNYGRDNPNHINVLSGNSIQHILYGDEAGGGHLFPGKPGKTTFPQHW
                     SASKITHEISDIVTSPKTQWYAQTGTGGKYIAKGRPARWVSYETRDGIRIRTVYEPAT
                     GKVVTAFPDRTSNPKYNPVK"
     gene            558..788
                     /locus_tag="WGQ68_002078"
     CDS             558..788
                     /locus_tag="WGQ68_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MafI family immunity protein"
                     /protein_id="HEZ0715803.1"
                     /translation="MKTLDERIKNLGKSLEDRIDANLIDAALEYITFSERLLAFETLC
                     DYIEDFNIQLTEKESQEISFINKEFGIESTSD"
     gene            937..>1292
                     /locus_tag="WGQ68_002079"
     CDS             937..>1292
                     /locus_tag="WGQ68_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003700253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HINT domain-containing protein"
                     /protein_id="HEZ0715804.1"
                     /translation="MLKTADGYKAIAHIRAGESVLSKDEASGKTGYKPVTARYGNPYQ
                     ETVYIEISDGIGNSQTLISNRIHPFYSDGKWIKAEDLKAGSRLLSENGRTQTVRNIVV
                     KPKPLKAYNLTVADWHT"
BASE COUNT          462 a          270 c          259 g          301 t
ORIGIN      
        1 gccgccaaag tcgcgaagct ggcaaaggcg gcgaaaccgg ggaaagctgc ggttagcggg
       61 gatttttctg ctgcatacaa tacaagaaca actagaaaag ttactacaga aacagagggg
      121 ttaaatagaa tcagacagaa ccagaaaaat agtaatatac atgagaaaaa ttatggaaga
      181 gataatccta atcatattaa tgttttatct ggaaattcta tacaacatat actgtatgga
      241 gatgaagcag gaggtgggca tctttttcct ggcaaacctg gtaagacaac attcccccaa
      301 cattggtcag ccagtaaaat aactcatgaa attagtgata tcgttacatc cccaaaaacg
      361 caatggtatg cacagactgg aacaggcggc aaatatattg ctaaaggaag accagccagg
      421 tgggtatcat atgaaacgag agatggaatt cgtatcagaa cagtttatga acctgcaaca
      481 ggaaaagtgg taactgcatt ccccgataga acctctaatc ccaaatataa ccctgtaaaa
      541 taatgagtat ttgaaagatg aaaacattag acgaacgaat aaaaaattta ggaaaatctc
      601 ttgaagatag aattgacgct aatctaattg atgccgcact agagtacatt actttttcag
      661 aacgtttgct agcttttgaa acgctatgtg attatataga agatttcaat atccagctta
      721 cggaaaaaga atcccaagaa atctctttta ttaataagga atttggaatt gaaagtacat
      781 cagattaata aatattaggt cggtttaaaa acccaacaat caaaaagtcg tctgaaactg
      841 tgtttaattt acagacgacc tttccttcat ttgaagcagg atattgagaa ctaagttctt
      901 caaaaatcct acacctactc cttccacggc agcaccttgc tcaaaacggc agacggctac
      961 aaagccattg cccatatccg agccggcgag agcgtcctct ccaaggacga ggcaagtgga
     1021 aaaacgggat acaaacccgt taccgcccga tacggcaatc cctatcaaga aaccgtttac
     1081 attgaaattt cagacggcat cggcaacagt caaaccctga tttccaaccg catccatccg
     1141 ttttattcgg acggcaaatg gattaaggcg gaagacttaa aggcgggaag cagactgtta
     1201 tccgaaaacg gcagaaccca aaccgtccgc aacatcgttg tcaaaccaaa accactcaaa
     1261 gcctacaatt tgaccgttgc cgattggcat ac
//
LOCUS       DAWFHY010000172         1266 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.172, whole genome shotgun sequence.
ACCESSION   DAWFHY010000172 DAWFHY010000000
VERSION     DAWFHY010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1266
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.172"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            319..>1266
                     /locus_tag="WGQ68_002080"
     CDS             319..>1266
                     /locus_tag="WGQ68_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002247879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0715805.1"
                     /translation="MNKTLKRRVFRHTALYAAILMFSHTGGGGAQAQTRNYAIVMNEQ
                     NQPEVKQEGSYSTLREKDRERKFIYNKGGSGGGSVSFNNSDELVSQQSGTAVFGTATY
                     LPPYGKVSGFDADGLKKRNNAVDWIRTTRIALAGYSYADIHCGSVQGCPKLVYKTRFS
                     FDNPDLEKTGGGLDRHTEPSRDNSPIYKLKDHPWLGVSFNLGSENTVKNSQSSSKLIS
                     SFSENNNNQTIVSTTEGSPISLGDWQREHTAVVYYLNAKLHLLDKKGIKDITGKTVRL
                     GVLKPSIDVKTQNTGLGLLNFWARWDIKDTGQIPVKLGLQ"
BASE COUNT          344 a          318 c          333 g          271 t
ORIGIN      
        1 tctgcagcgg tttgctaaaa aacgctttac cgtgatcagt gtgcaaagtt aaaatgggga
       61 ggtaagcttt tcaaccagca attcggcggg cgcgggatcg ggcggtttgc cgaatcccgc
      121 cgtttgcggc gcgcctgccg ccgacggtat cccgcgaagc aagatttaag ggataaaata
      181 tgttccaaca cacagggcgg cacataaggc gccgccctga ttcggaaggg cttgcacccc
      241 tcccggacaa agcctgatcc tgccgccccg aaggacggat gcccgaaggg cggggggttt
      301 gaccgaaaag gaaatacgat gaataaaact ttaaaaaggc gggttttccg ccataccgcg
      361 ctttatgccg ccatcttgat gttttcccat accggcgggg ggggggcgca ggcgcaaacc
      421 cgtaactacg ctattgtcat gaacgaacaa aatcagccgg aggtaaagca ggagggttca
      481 tattcaacct taagggaaaa agacagggaa cgcaaattta tctataataa aggcggatcg
      541 ggtggaggct ctgtctcatt caacaatagc gatgagcttg tttcccaaca aagcggtact
      601 gccgtttttg gcacagccac ctacctgccg ccctacggca aggtttccgg ttttgatgcc
      661 gacgggctga aaaagcgcaa caatgccgtc gattggattc gtaccacccg catcgcgctg
      721 gcaggctaca gctacgccga tatccattgc ggaagtgtcc aaggctgtcc caaacttgtc
      781 tataaaaccc gattttcctt cgataatccc gacttggaaa aaacaggagg cgggctggat
      841 aggcacacag agccaagccg cgacaattcg cccatttaca aattgaaaga tcatccatgg
      901 ttgggcgtat ctttcaattt gggcagcgag aataccgtca aaaatagcca atcatccagc
      961 aaattgatat cttcttttag tgaaaacaat aataatcaaa ccatcgtctc tacgacagaa
     1021 ggctccccta tttcccttgg cgactggcag cgcgaacata ccgccgtggt ctattatctg
     1081 aacgccaaac tgcacctgct ggataaaaaa gggattaaag atatcaccgg caaaacagtg
     1141 cggttgggtg tcttgaagcc gagcatcgat gtgaagacac aaaatacggg gcttggcttg
     1201 ctaaattttt gggctaggtg ggacattaaa gataccgggc agattccggt caagctcggc
     1261 ctgcag
//
LOCUS       DAWFHY010000173         1224 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.173, whole genome shotgun sequence.
ACCESSION   DAWFHY010000173 DAWFHY010000000
VERSION     DAWFHY010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1224
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.173"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>1224
                     /locus_tag="WGQ68_002081"
     CDS             <1..>1224
                     /locus_tag="WGQ68_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0715806.1"
                     /translation="RLEKFDRVALQHFSQYVDLINENNGRLPNTSEIERSYYKAVTEN
                     GVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNAQAVNNPNGSEWDNRKQLISA
                     LDKGFDGSFKEKHFTFLQSVIMDVTKLGVEYTIDGWQKIGGWGNGIINDLYKSVAKRE
                     WTGIFEIVNNNIKQFRDLFPNPEGWIDDGHQCFAPWVKETKKRNGKYHVYDPLALDLD
                     GDGIETVATKGFSGALFDHRNQGIRTATGWVSADDGLLVRDLNGNGIIDNGTELFGDN
                     TKLADGSFAKHGYAALAELDSNGDNIINAADAAFQTLRVWQDLNQDGISQTNELRTLE
                     ELGIQSLDLAYKDVNKNLGNGNTLAQQGSYTKTNGTTAKMGDLLLAADNLHSRFKDKV
                     ELTAEQAKAANLAGIG"
BASE COUNT          384 a          251 c          282 g          307 t
ORIGIN      
        1 ccgtttagag aaatttgatc gggttgcatt gcaacatttc agccaatatg tagatctaat
       61 taatgaaaat aatggtagat tacctaacac tagtgaaatt gagagaagtt actataaagc
      121 cgttaccgaa aatggtgttt cttctagtgc agctattgat ttagttatta atcgctcact
      181 tccggatatg gcagatggtt attgggcatt aggtttgggg atagaagccg aacgtatcca
      241 caatgcgcaa gcagtaaata atccgaacgg tagcgaatgg gataatagaa agcagttaat
      301 atctgcttta gataaaggat ttgatggatc ttttaaagag aagcatttta cttttttaca
      361 atctgtgata atggatgtaa caaagttagg tgttgaatat acaatagatg gttggcaaaa
      421 aattggaggt tggggtaatg ggataatcaa tgatttatat aaaagtgttg caaaaagaga
      481 gtggactgga atatttgaga tcgttaataa taacatcaag caatttagag atctgttccc
      541 aaatccggaa ggctggatcg atgatggtca ccaatgtttc gctccttggg ttaaagaaac
      601 taaaaaacgc aatggcaaat atcatgtcta cgaccccctc gccctagacc tagacggcga
      661 cggcatagaa acagtcgcca ccaaaggctt ttcaggtgca ttgttcgacc accgcaacca
      721 aggcatccgc accgccaccg gttgggtttc tgccgatgac ggtctgcttg tgcgcgattt
      781 gaacggcaac ggcatcatcg acaacggcac ggaactcttc ggcgacaaca ccaaactggc
      841 agacggttct tttgccaaac acggctatgc agctttggcc gaattggatt caaacggcga
      901 caacatcatc aacgcggcag acgccgcatt ccaaaccctg cgtgtatggc aggatctcaa
      961 tcaggacggc atttcccaaa ccaacgaact ccgcaccctt gaagaattgg gtattcaatc
     1021 tttggatctc gcctataaag atgtaaataa aaatctcggt aacggtaaca ctttggctca
     1081 gcaaggtagc tataccaaaa caaacggtac aaccgcaaaa atgggggatt tacttttagc
     1141 agccgacaat ctgcacagcc gttttaaaga taaagtggaa ctcactgccg aacaggcaaa
     1201 agccgccaat cttgcgggca ttgg
//
LOCUS       DAWFHY010000174         1132 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.174, whole genome shotgun sequence.
ACCESSION   DAWFHY010000174 DAWFHY010000000
VERSION     DAWFHY010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1132
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.174"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(235..1089)
                     /locus_tag="WGQ68_002082"
     CDS             complement(235..1089)
                     /locus_tag="WGQ68_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEZ0715807.1"
                     /translation="MFYQILALIIWSSSFIAAKYVYGGIDPALMVGVRLLIAALPALP
                     ACRRHVGKIPREEWKPLLIVSFVNYVLTLLLQFVGLKYTSAASASVIVGLEPLLMVFV
                     GHFFFNDKARAYHWICGAAAFAGVALLMAGGAEEGGEVGWFGCLLVLLAGAGFCAAMR
                     PTQRLIARIGAPAFTSVSIAAASLMCLPFSLALAQSYTVDWSVGMVLSLLYLGLGCGW
                     YAYWLWNKGMSRVPANVSGLLISLEPVVGVLLAVLILGEHLSPVSALGVFVVIAATLV
                     AGRLSHQK"
BASE COUNT          319 a          339 c          293 g          181 t
ORIGIN      
        1 aaaatcccgt cattcccgcg caggcggaaa tccggacatc caacgctgca gcaatttgtc
       61 ggaaataacc gaaactcaaa aaaccggatg cccgcctgcg cgggaatgac gacgcaaaga
      121 tggtcgttat ttcggataaa tatccgtctt ttcgttccgg atacccactt tcgcaggaat
      181 ggagatggaa agatttcagc ctattcggca atcatcaaat accgcttccc aactttattt
      241 ttgatgcgac agccggccgg caaccaaggt ggcggcgatg acgacaaaca cgcccaaggc
      301 ggacacgggc gacaggtgtt cgcccaaaat caaaaccgcc agcagcacgc cgacgacggg
      361 ttcgagcgaa atcaacagtc ccgaaacatt ggcaggaaca cggctcatcc ccttgttcca
      421 cagccaatag gcgtaccagc cgcaccccaa acccaaatac agcagcgaca ataccatccc
      481 gacgctccag tccacggtat aactttgcgc caaagcaagc gaaaacggca ggcacatcaa
      541 cgatgcggcg gcaatggaaa cagatgtgaa tgccggtgcg ccgatgcgtg caatcagcct
      601 ttgcgtcgga cgcatagcgg cacaaaagcc cgcgcccgcc aacaacacca gcaggcagcc
      661 gaaccagccg acttcgccgc cctcttccgc accgcccgcc atcagcagcg cgacaccggc
      721 aaatgccgcc gcgccgcata tccagtggta ggcacgcgct ttgtcgttga agaaaaagtg
      781 tccgacaaac accatcagca gcggctcgag tccgacaatg accgatgcgc tggcggcgga
      841 agtgtatttc aacccgacaa actgaagcag cagggtcagc acatagttga cgaacgacac
      901 aatcagcaac ggcttccatt cctcacgcgg aatcttgccg acatgacggc ggcaggcggg
      961 cagtgcaggc agcgcggcaa ttagcaggcg cacgccgacc atcaatgcgg gatcgatgcc
     1021 gccatagaca tatttggcgg caataaacga gctgctccag ataatcaggg caaggatttg
     1081 gtaaaacata gtgagtgata aagtcagatg gtcatacaaa agtatagcgg at
//
LOCUS       DAWFHY010000175         1105 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.175, whole genome shotgun sequence.
ACCESSION   DAWFHY010000175 DAWFHY010000000
VERSION     DAWFHY010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.175"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            219..1016
                     /gene="truA"
                     /locus_tag="WGQ68_002083"
     CDS             219..1016
                     /gene="truA"
                     /locus_tag="WGQ68_002083"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003703378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEZ0715808.1"
                     /translation="MDTAQKQRWAITLSYDGSRFYGWQKQADGVPTVQAALETALAQI
                     AGEAVSTTVAGRTDTGVHATAQVVHFDTAAVRPVQAWVRGVNAHLPEGIAVLHARQVA
                     PEFHARFDAYGRHYRYLLESAPVRSPLLKNRAGWTHLKLDIGQMRQAAALLIGEQDFS
                     SFRAAECQAKSPVKTIYRADLTQSSGLVRLDLHGNAFLHHMVRNIMGALVYVGSGRLS
                     VEGFAALIQERSRLKAPPTFMPDGLYLTGVDYPEAYGIIRPQIPEWL"
BASE COUNT          253 a          371 c          257 g          224 t
ORIGIN      
        1 atggtttctt tagattttac gtcctagatt cccgcctgcg cgggaatgac gattcgggca
       61 ctcctgacag ggtaaattca caggatagcg attcgtagca actgcatccc cccccccaac
      121 aactccccaa acaacgccgc tcgccctggg cgtttgccgt ttccctgcaa aatctgcgat
      181 acaatgcagt ctgaacattt atccgaatcc caaatccgat ggataccgca caaaaacaac
      241 gctgggcaat aaccctatcc tatgacggca gccgctttta cggctggcag aaacaggctg
      301 acggcgtacc gaccgttcag gcggcattgg aaaccgcgct cgcccaaata gcaggggaag
      361 cggtttccac caccgttgcc ggcaggaccg ataccggcgt tcatgcgact gcccaagtcg
      421 ttcattttga tactgccgcc gtccgtccgg ttcaggcatg ggtgcgcggc gtaaatgccc
      481 acctgcccga aggcattgcc gttttgcacg cccgacaggt cgcccccgaa tttcacgccc
      541 gcttcgacgc atacggacgg cattaccgct acctgctcga atccgccccc gtccgttccc
      601 ccctgctcaa aaacagggca ggctggacac acctcaaact cgacatcggg cagatgcggc
      661 aggctgccgc cttattgatc ggcgaacaag acttctccag cttccgcgcc gccgaatgcc
      721 aggcaaaatc ccccgtcaaa accatctacc gcgccgacct tacccaaagc tcaggactcg
      781 tccgcctcga tttgcacggc aacgcctttt tgcaccatat ggtacgcaac atcatgggcg
      841 cgctcgtcta tgtcggcagc ggcagactca gcgtcgaagg cttcgccgca ctgattcaag
      901 aacgcagccg cctcaaagcc ccgccgacct tcatgcccga cggactttac ctgaccggcg
      961 tcgactatcc cgaggcatac ggcatcatcc gcccccaaat ccccgaatgg ctttaaaaca
     1021 ttcttgtcgc ggagattttg aaatcggaca aactgtcagg caatcttttt caatattgac
     1081 actacctcat caaggtaata acatt
//
LOCUS       DAWFHY010000176         1093 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.176, whole genome shotgun sequence.
ACCESSION   DAWFHY010000176 DAWFHY010000000
VERSION     DAWFHY010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1093
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.176"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            284..721
                     /locus_tag="WGQ68_002084"
     CDS             284..721
                     /locus_tag="WGQ68_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003711106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VapD"
                     /protein_id="HEZ0715809.1"
                     /translation="MSRYLITFDMDTNCLKNNFHGNSYNNAYADIKHILARHGFEKIQ
                     RSVYLGREGISEAHGTIAIQELTARFDWFSSCISNIKFYRIESDLNAQFIADGVYQAK
                     QAFLQRVEQLRISLTEAGLSDEQINQVLEKQKFELESPNLKLN"
BASE COUNT          339 a          245 c          225 g          284 t
ORIGIN      
        1 gccaccaaaa cccctgctag ctaagggtta aacagctcac ttgaaatcta cttaagtcta
       61 atctaaacta tccaatatgg atagattttt aaacataggg caagcagtaa aattattgta
      121 gctgaaagca caatcactcg ctggtggtct caaacacgtg ccgactacct cgccgaaaac
      181 actatcagct gcgataaacc gtgggaaaag ctcggtatca tgcgccgcac ttggtattat
      241 cgcgggaaac cgatgccgtc tgaaacgcaa caggagaaaa ataatgagcc gttacctgat
      301 tacctttgac atggatacca actgtctgaa aaacaatttc cacggaaata gctataacaa
      361 tgcctatgcg gatattaaac acatcttggc tagacatgga tttgaaaaga ttcagcgcag
      421 tgtttatcta ggccgtgaag gcatcagtga agcacacgga acaatagcca ttcaggaact
      481 gaccgctcgg tttgattggt tttcctcctg tatttcaaac attaagtttt accgcattga
      541 aagcgatttg aacgcacaat ttatcgctga tggtgtgtat caagccaaac aggctttcct
      601 tcaacgtgtt gaacaacttc gtatatccct aacagaagct ggattgtctg atgagcaaat
      661 caatcaggtt ctggaaaaac agaaatttga attggaaagt cctaacctga aattaaatta
      721 acctccttta ctctccaaca tccgccgcag ttctgtcagt ttttggcgcg ctgcggcgtt
      781 ttctgtgcgt tttagagctt cgggtagggt gtgaaacagc tcactcgaaa tctacttaag
      841 tctaatctaa actatccaag cagtaattag tacaaaaaaa gcaaacttat tttaggagct
      901 tatgatgact gaaaaattgc agccgcgata aaccgtggga gagtctcggc atttcccacg
      961 ccacttggta caaacgtggc aaaccgatgc cgtctgaaac cgtacaacag gagacaaaat
     1021 aatgcttgct caaattgaac tcacaccctg ccagctaatg gtttccgtaa cttaaggtta
     1081 ctgggtttgc gca
//
LOCUS       DAWFHY010000177         1073 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.177, whole genome shotgun sequence.
ACCESSION   DAWFHY010000177 DAWFHY010000000
VERSION     DAWFHY010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.177"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            207..>1073
                     /locus_tag="WGQ68_002085"
     CDS             207..>1073
                     /locus_tag="WGQ68_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002253430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly/adherence protein PilC"
                     /protein_id="HEZ0715810.1"
                     /translation="MNAQKLPEVKWGNDYSSLTDKSNEREVTHTSKFIVTKKHISFSF
                     NNNDEVVAEKKDTVVFGAATYLPPYGKVSGFDTTKLTERKNAVDQIGTTHPGLIGYSY
                     QGSTCSSSSCPEVVYRTQFTFGNSSLAKNKTDNKLDIYEDKSRDNSPIYKLKDHPWLG
                     VSFSLGGESSFKPKRQGSLVSSFSEDVTQNNNNQHKDKNLVYTTDDYNSKGNKNHQDK
                     HHAVAFYLNAKLHLLDKKQIKNIAQVGTVDLGTLKTRIEPTEAWKKQNNNFFSGSWTY
                     EEKGSVSVKLKLP"
BASE COUNT          348 a          245 c          250 g          230 t
ORIGIN      
        1 atcgggcggt ttaccgaagc ccggcgttcg cggcgcgccg ccccgtcccg tgaaggcaaa
       61 ctcaaggaat aaaagatgaa taaaacttgg aaacggcagg ttttccgcca taccgcgctt
      121 tataccgcca tattgatgtt ttcccatacc ggcggggggg ggggcaggcg caagcgcaaa
      181 cgcaaacgca taaatacgct attgtaatga acgcgcaaaa gctgcctgag gtaaagtggg
      241 ggaacgacta tagctcattg acggacaaaa gcaatgaacg cgaagttacc catacgagta
      301 aatttattgt aacaaaaaag cacattagtt tctcattcaa taataacgat gaagttgttg
      361 ctgaaaaaaa agatactgtc gttttcggcg cggcgactta tctgccgccc tacggcaagg
      421 tttccggttt tgataccact aagctgaccg agcgcaaaaa tgccgttgat cagattggta
      481 cgacccaccc ggggctgata ggctatagct accaaggtag cacatgctcc agcagcagct
      541 gtcctgaagt tgtctataga acccaattta ccttcggcaa ttccagtttg gcaaaaaata
      601 aaacagacaa caagctggat atatacgaag acaaaagccg cgacaattcg cccatttaca
      661 aattgaagga tcatccttgg ttgggcgtgt ctttcagttt gggcggagag agctccttca
      721 aaccaaagag acaaggttct ttggtatctt cttttagcga ggatgtgacg cagaacaata
      781 acaaccaaca caaagacaaa aacctcgttt atacgacaga cgattacaat agtaagggta
      841 ataaaaacca tcaggacaaa caccacgccg tcgcctttta tctgaacgcc aaactgcacc
      901 tgctggataa aaaacaaatc aaaaatatcg cgcaagtcgg aacagttgat ttgggtacct
      961 tgaaaacacg catcgagccg acggaagcat ggaaaaaaca gaataataac tttttcagcg
     1021 gtagttggac gtatgaagag aaaggatcag tcagcgtcaa actcaaattg ccg
//
LOCUS       DAWFHY010000178          946 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.178, whole genome shotgun sequence.
ACCESSION   DAWFHY010000178 DAWFHY010000000
VERSION     DAWFHY010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.178"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..216)
                     /locus_tag="WGQ68_002086"
     CDS             complement(<1..216)
                     /locus_tag="WGQ68_002086"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715811.1"
                     /translation="MPSVELLLVIVGLPRGTFYYQLVVQSAEDKYADLKRHIHDIYQK
                     QLKDNGLVQSMSRKGNCLDNAAMESFFG"
     gene            complement(248..664)
                     /locus_tag="WGQ68_002087"
                     /pseudo
     CDS             complement(248..664)
                     /locus_tag="WGQ68_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360042.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          224 a          231 c          204 g          287 t
ORIGIN      
        1 tccgaagaaa ctttccattg ccgcattgtc caagcagttt cccttgcggg acatactctg
       61 aaccagaccg ttgtctttca actgcttttg ataaatatca tggatatgcc gtttcaaatc
      121 ggcatatttg tcttctgccg attggacaac caattggtaa tagaaggtgc cgcgtggcag
      181 tccgacaatc accaacagca gttcaacgga tggcattgcc ttaaccctgc gacgagttgc
      241 gttctttcta ccgcacttct ttcccataga ttaaggcatc gagctttttt agggcagcca
      301 tttccgcttt aaggcaagcc aattccgcaa gcagttcttc cttggttttc agatagtcgg
      361 ctttttcgtt tccggcggat gctgtttttt cacgggcttt cttcctttgg gtttagggtt
      421 tgggctttaa accgttaata ccattcaaat ggtagaggcg caaccattgc agcaagatgg
      481 agcagtcggg caaattcagt tggtctgcgg cagctttttg ggacattccc taccccgtca
      541 ccaggcggat tgcctcaagt ttgtattcga ccgaatattt tgtcgtatgc tttctacgtt
      601 tgatgccact ctctccgtgt aatctgtatt ttgtcaccca tctgcgtacc aatgaatcgg
      661 aaatagaaag atggtctgct gttccctgcc aaatagtatt gaacgacggc aagtcggaat
      721 tcatctgaac attttgccat aaaaaactga acctcctaaa gtcggtaagg tgtctcactt
      781 tggggtgcag ttcagaagcg gtcttttttg cctgccggtt ttgaatcatc ctccgtgtat
      841 attcccttga cgaaaaaaat gatgatatta cggatactaa aactaaggtc atatcccccc
      901 tactctccct aagcaaagag atgaaacagc gtatcggtcc cctacc
//
LOCUS       DAWFHY010000179          941 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.179, whole genome shotgun sequence.
ACCESSION   DAWFHY010000179 DAWFHY010000000
VERSION     DAWFHY010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.179"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..114
                     /locus_tag="WGQ68_002088"
     CDS             <1..114
                     /locus_tag="WGQ68_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0715812.1"
                     /translation="PFFISNDTDLALFKTTKWGICRAIADAIAMELGAAKV"
     gene            111..284
                     /locus_tag="WGQ68_002089"
     CDS             111..284
                     /locus_tag="WGQ68_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002245587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715813.1"
                     /translation="MKKSLIALALSVLKPQMPEFEIKPASIGYLKQHPSMRLGKSGVA
                     AAKRAARKRKNRR"
     gene            287..451
                     /locus_tag="WGQ68_002090"
     CDS             287..451
                     /locus_tag="WGQ68_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003751173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715814.1"
                     /translation="MGQVEFYEKMIGLWSAKSREASEQADLAAFEFAEGELANYQEML
                     KRHLQTKSVE"
     gene            454..690
                     /locus_tag="WGQ68_002091"
     CDS             454..690
                     /locus_tag="WGQ68_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002236958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715815.1"
                     /translation="MRILDIFKNPATGNVSHSKLWANVACAAGTFKFVMLPDPSAEIW
                     AVYLGIVGGYAVARSFVSVKRQEVENESRETAGE"
     gene            662..>941
                     /locus_tag="WGQ68_002092"
     CDS             662..>941
                     /locus_tag="WGQ68_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715816.1"
                     /translation="MNLVKLLANNWQPIAIIALIGTGLAVSHHQGYYKSAFAKQQAVI
                     DKMEKDKAQALLLSAQNYARELEQARAEAKKYEVKAHAVGMALAKKQAE"
BASE COUNT          235 a          205 c          290 g          211 t
ORIGIN      
        1 ccgtttttta tcagcaacga cactgatttg gccttgttta agacgaccaa atggggcatc
       61 tgccgcgcga ttgcggacgc gattgcgatg gaattgggag cggcgaaggt atgaaaaagt
      121 ctttgattgc tttggctctg tctgtcttga aaccgcagat gcctgaattt gagattaagc
      181 ctgccagtat tggctatttg aaacaacatc cgtctatgcg cctgggcaag tcgggcgtag
      241 cggctgcgaa acgcgcggcg cgtaaacgca agaatcgtcg ttaatcatgg gacaggttga
      301 gttttacgaa aagatgattg ggctgtggtc ggccaaaagc cgtgaggcaa gtgaacaggc
      361 agacttggcg gcgtttgaat ttgcggaggg cgaactggcc aattatcagg aaatgctgaa
      421 acggcacctg caaaccaaaa gtgtggaata gcaatgcgta ttttggatat ttttaaaaac
      481 cccgcgacag gcaatgtgtc gcactcgaaa ctgtgggcaa acgttgcctg cgcggcgggg
      541 acgtttaagt ttgtgatgct gcccgatccg tcggcggaga tttgggcggt gtatttgggc
      601 attgtcggcg gctatgcggt ggcgcgctcg tttgtcagcg tcaaacgtca ggaggtcgag
      661 aatgaatctc gtgaaactgc tggcgaataa ctggcaacca attgccatca tcgcacttat
      721 cggcacgggt ttggcggtgt cgcaccatca aggctattat aagtcggcgt ttgcgaagca
      781 gcaggcggtc attgacaaga tggaaaaaga caaagcgcaa gccctgctgt tgtcggctca
      841 aaactacgcc cgcgagctgg aacaggcgcg tgcggaagct aaaaaatatg aagtcaaggc
      901 gcacgccgtc ggcatggctt tggcgaaaaa acaggcggaa g
//
LOCUS       DAWFHY010000180          940 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.180, whole genome shotgun sequence.
ACCESSION   DAWFHY010000180 DAWFHY010000000
VERSION     DAWFHY010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.180"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(379..702)
                     /locus_tag="WGQ68_002093"
     CDS             complement(379..702)
                     /locus_tag="WGQ68_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002214977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEZ0715817.1"
                     /translation="MDMVVRGGIYLVSLDPTVGSEIKKTRPCVVVSPPEIHNYLKTVL
                     IVPMTSGSRPAPFRVNVRFQDKDGLLLPEQIRAVDKAGLVKHLGNLDNSTAEKLFAVL
                     QEMFA"
     gene            complement(690..929)
                     /locus_tag="WGQ68_002094"
     CDS             complement(690..929)
                     /locus_tag="WGQ68_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002227726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="growth regulator"
                     /protein_id="HEZ0715818.1"
                     /translation="MILNIRKMGNSQGVILPKSLLGQIGAVDSLAVTVEKGNIILSCP
                     TVRRGWAEAAAMLVETEQEHFFSEIENEADKEWIW"
BASE COUNT          257 a          236 c          199 g          248 t
ORIGIN      
        1 ttacgaacct acatcccgtc attccctcaa aaacagaaaa ccaaaattag aaacctaaaa
       61 tcccgtcatt cccgcgcagg cgggaatcta ggtctgtcgg tgtggaaact tatcgggtaa
      121 aacggtttct ttagattcca cgttctagat tcccgcctgc gcgggaatga tggctgcaga
      181 tgcccgacgg tctttatagt ggattaacaa aaatcaggac aaggcgacga agccgcagac
      241 agtacaaata gtacggaacc gattcacttg gtgcttcaac accttagaga atcgttctct
      301 ttgagctaag gcgaggcaac gccgtactgg tttaaagtta atccactata atgaacacaa
      361 tccattcaga ctattcaatc aggcaaacat ctcctgcaat actgcaaaca gtttttcagc
      421 cgtactgttg tctaaatttc caagatgttt gaccaatccg gctttatcca cagccctaat
      481 ctgttcgggc aaaagcaaac cgtctttatc ctgaaagcgg acattgacgc ggaacggggc
      541 aggacggctt ccgctcgtca taggaacgat cagcacagtc ttgagatagt tgtgtatttc
      601 aggaggagat actacgacac aaggacgtgt ctttttgatt tcgcttccta cggtcgggtc
      661 taaggagacc agatagattc cgccgcgtac taccatatcc attctttatc cgcttcgttt
      721 tcaatttcgg aaaaaaaatg ctcctgctcg gtttcgacaa gcattgcggc agcttctgcc
      781 catcccctgc gaacggtagg acagcttaaa atgatattgc ccttttcaac tgtaacagcc
      841 aagctgtcta ctgcccctat ttgacccaat aatgatttgg gcagaatcac gccttgcgag
      901 tttcccattt tgcgtatgtt gagaatcata tacgtaccct
//
LOCUS       DAWFHY010000181          920 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.181, whole genome shotgun sequence.
ACCESSION   DAWFHY010000181 DAWFHY010000000
VERSION     DAWFHY010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..920
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.181"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            119..920
                     /locus_tag="WGQ68_002095"
                     /pseudo
     CDS             119..920
                     /locus_tag="WGQ68_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014582428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT          269 a          240 c          222 g          189 t
ORIGIN      
        1 ctcgacttag ctcaaagagc gattctctaa ggtgctgaag caccaagtga atcggttccg
       61 tactatttgt actgtctgcg gcttcgtcgc cttgtcctga tttttgttaa tccaatatac
      121 tttatgccgc taccgcaacc gactggctca agacaatacc ctgtctgaac tgttggaact
      181 gattaaccgc caactgaccg aaaaaggttt aaaaatagag aaagcatccg ccgccgtcgt
      241 tgacgccacc attattcaga ccgctggcag caaacagcgt caggccatag aagttgacga
      301 agaaggacaa atcagcggcc aaaccacacc gagtaaagac agcgatgccc gttggataaa
      361 gaaaaacggc ctctacaaac tcggttacaa acaacatacc cgtaccgatg tggaaggcta
      421 tatcgagaaa ctgcacatta cccccgccaa tgcccatgag tgcaaacacc tgtcgccgtt
      481 gttggaagga ctgcccaaag gtacgaccgt ctatgccgac aaaggctacg acagtgcgga
      541 aaaccggcaa catctggaag aacatcagtt gctggacggc attatgcgca aagcctgccg
      601 caaccgtccg ctgtcggaaa cgcaaaccaa acgcaaccgg tatttgtcga agacccgtta
      661 tagtggatta aatttaaatc aggacaaggc gacgaagccg cagacagtac aaatagtacg
      721 gcaaggcgag gcaacgccgt actggtttaa atttaatcca ctatatgtgg tcgaacagag
      781 cttcggtacg ctgcaccgta aattccgcta tgcgcgggca gcctatttcg gactgattaa
      841 agtgagtgcg caaagccatc tgaaggcgat gtgtttgaac ctgttgaaag ccgccaacag
      901 gctaagtgtg cccgctgccg
//
LOCUS       DAWFHY010000182          911 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.182, whole genome shotgun sequence.
ACCESSION   DAWFHY010000182 DAWFHY010000000
VERSION     DAWFHY010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.182"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>911
                     /locus_tag="WGQ68_002096"
     CDS             <1..>911
                     /locus_tag="WGQ68_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0715819.1"
                     /translation="IEAGRKLTLYAVEELNYDKLDSQKRRRFLGISYSKAHDTTTQVM
                     KTALPSRVVAESANLQSGWDTKLQGTQFETTLGGATIRAGVGEQARADAKIILEGIKT
                     TIHNETVSSSKSALWQKQAGRGSNIETLQLPSFTGPVAPVLSAPGGYIADIPKGNLKT
                     EIETLTKQPEYAYLKQLQVAKNINWNQVQLAYDKWDYKQEGLTEAGAAIIALAVTVVT
                     SGAGTGAVLGLNGAAAAATDAAFASLASQASVSFINNKGDVGKTLKELGRSSTVKNLV
                     VAAATAGVADKIGASALNNVSDKQWIN"
BASE COUNT          268 a          258 c          232 g          153 t
ORIGIN      
        1 catcgaagcc gggcggaaat tgacactcta tgccgtagaa gagctcaact acgacaaact
       61 tgacagccaa aaaaggcgca gatttcttgg catcagctac agcaaagcac acgacaccac
      121 cacccaagtc atgaaaaccg cgctgccctc aagggtagtt gcagaatcag ccaatctgca
      181 atcaggttgg gataccaaac tgcaaggcac acagtttgaa accacactgg gtggcgcaac
      241 catacgcgca ggcgtaggtg agcaggcacg ggcagatgcc aagattatcc tcgaaggcat
      301 caaaaccacg atccacaacg aaaccgtgag cagcagcaaa tctgctctat ggcaaaaaca
      361 ggcaggacgg ggcagtaaca tcgaaacctt gcaattgccg agtttcaccg gtcccgttgc
      421 gcccgtactg tccgctcccg gcggctatat cgccgacatc cccaaaggca acctcaaaac
      481 cgaaatcgaa accctcacca agcagcccga gtatgcttat ttaaaacagc ttcaagttgc
      541 gaaaaacatc aactggaatc aggtgcagct tgcttacgat aaatgggact acaaacagga
      601 gggcttaacc gaagcaggtg cggcgattat cgcactggcc gttaccgtgg tcacctcagg
      661 cgcaggaacc ggagccgtat tgggattaaa cggtgcggcc gccgccgcaa ccgatgcagc
      721 atttgcctct ttggccagcc aggcttccgt atcgttcatc aacaacaaag gcgatgtcgg
      781 caaaaccctg aaagagctgg gcagaagcag cacggtgaaa aatctggtgg ttgccgccgc
      841 taccgcaggc gtagccgaca aaatcggcgc ttcggcactg aacaatgtca gcgataagca
      901 gtggatcaac a
//
LOCUS       DAWFHY010000183          873 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.183, whole genome shotgun sequence.
ACCESSION   DAWFHY010000183 DAWFHY010000000
VERSION     DAWFHY010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.183"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(32..463)
                     /locus_tag="WGQ68_002097"
     CDS             complement(32..463)
                     /locus_tag="WGQ68_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715820.1"
                     /translation="MKNNIFLNLNKKSINNNHFVISIFFETIYQFETKDTLLECFKNI
                     TTTGHFGVIGAQYEKIDATRWIGDYEEVNGFEYIDKAPSIYFSVGDDFNPEELIIPIN
                     LAYHYFNIAISDFLIAHPEYQKKCKEIQKTYSQTNCSLHET"
     gene            complement(479..>873)
                     /locus_tag="WGQ68_002098"
     CDS             complement(479..>873)
                     /locus_tag="WGQ68_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EndoU domain-containing protein"
                     /protein_id="HEZ0715821.1"
                     /translation="HNRTNFMAELNSRGGRVKSETQTDIEGITRIKYEIPTLDRTGKP
                     DGGFKEISSIKTVYDPKKFSDDKILQMAQNAASQGYSKASKIAQNERTKSISERKNVI
                     QFSETFDGIKFRSYFDVNTGRITNIHPE"
BASE COUNT          264 a          129 c          132 g          348 t
ORIGIN      
        1 cgtgctgaag cacacacctt acgcatggat tttaggtttc atgcaggcta cagtttgttt
       61 gagaatatgt tttttgtatt tctttacact ttttttgata ttcagggtga gctattaaga
      121 aatcagatat tgcaatatta aagtaatgat atgctaaatt aataggtata attaattctt
      181 caggattgaa atcatctcca actgaaaaat aaatagaagg agctttatca atatactcaa
      241 atccatttac ctcttcataa tctccaatcc atctggtagc atctattttt tcatattgag
      301 cacctattac tccaaaatgt ccggtagttg taatattttt aaaacactct aaaagcgtat
      361 ctttagtttc aaattggtaa attgtttcaa aaaaaatcga aataacaaaa tggttgttat
      421 ttatagattt tttatttaag tttagaaaaa tattattttt cataattttt cctttaaatt
      481 attctgggtg aatgtttgta attcttcctg tatttacatc aaaatatgat ctaaatttga
      541 ttccgtcaaa ggtttctgag aattgaatga cattttttct ttccgatatt gatttagttc
      601 tttcattttg agcaatttta gaggcttttg aatatccttg tgaagcagca ttttgagcca
      661 tttgaagtat tttatcatca gaaaattttt taggatcata aacagttttt atacttgaaa
      721 tttccttaaa tccaccatca ggtttacctg tcctgtctag tgtaggaatc tcatatttaa
      781 ttcgggtaat gccttcaata tcagtttggg tttcagactt tacgcgtcct cctcgtgaat
      841 ttagttctgc cataaaattg gtgcggttat ggg
//
LOCUS       DAWFHY010000184          832 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.184, whole genome shotgun sequence.
ACCESSION   DAWFHY010000184 DAWFHY010000000
VERSION     DAWFHY010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.184"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(10..576)
                     /locus_tag="WGQ68_002099"
     CDS             complement(10..576)
                     /locus_tag="WGQ68_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HEZ0715822.1"
                     /translation="MSLYALLLIALGMSMDAFAVALAKGAAVRMPPRKIAATALVFGS
                     VEALTPLAGWVGGFYAKPFISEWDHWAAFVLLGGLGLKMMREGLSGKAEDVRESKRES
                     LWMTVLTAFGTSIDSMIVGVGLAFMEVNIAFAAAIIGMATTVMVAVGLTAGRALGVLF
                     GRRAEFAGGLVLIAIGTWTLLSHLGLIQ"
BASE COUNT          231 a          269 c          175 g          157 t
ORIGIN      
        1 tttccgacat cattgaatca aacccaaatg cgacaagagc gtccatgtac cgatggcaat
       61 caacaccaaa cctccggcaa attccgcacg cctgccgaac aatacgccca aagcccttcc
      121 cgccgtcagc ccgaccgcca ccatcaccgt cgtcgccata ccgatgattg cggcggcaaa
      181 ggcgatgttt acctccataa acgccaagcc caccccgact atcatggaat caatactggt
      241 tccaaaagca gtcaaaaccg tcatccatag gctttcccgt ttgctttcgc gcacatcttc
      301 cgccttgccg gacagccctt cgcgcatcat tttcagaccc agcccgccca gcaggacgaa
      361 agccgcccaa tggtcccatt cgctgataaa cggtttggca taaaaaccgc ctacccagcc
      421 tgccagcggc gtgagtgctt caaccgagcc gaacaccaag gctgttgctg caattttgcg
      481 cggaggcatc ctgaccgccg caccctttgc caatgcgacg gcaaacgcat ccatcgacat
      541 ccccagcgca atcaagagca aagcgtaaag actcataccg cacccatcct caaaaagggc
      601 agattatagc aaaacagcaa agcgcgaaaa acgccgcacg aaaacccaca tcccgtcatt
      661 cctgcaaaaa caaaaaatca aaaacagaaa tcccgtcatt cccgcgcagg cgggaatcca
      721 gaccggtcgg tgcagaaact tattggataa aacggtttct ttagatttta cgttctagat
      781 tcccactttc gtgggaatga cgggatgtag gtttccgtgc ggatggattc gt
//
LOCUS       DAWFHY010000185          788 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.185, whole genome shotgun sequence.
ACCESSION   DAWFHY010000185 DAWFHY010000000
VERSION     DAWFHY010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.185"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..345)
                     /locus_tag="WGQ68_002100"
     CDS             complement(<1..345)
                     /locus_tag="WGQ68_002100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715823.1"
                     /translation="MAWWEFRGFWGFCKGLNLQIGFKTIRPNSSASKNGMPIYSNVSR
                     REIFDLYEQYSGQRPDFRNIPNKGQLSSTTITSGGGFAFEVQLSLTEKGQYAVAYGLS
                     CKKDCHELHATDP"
     gene            360..>788
                     /locus_tag="WGQ68_002101"
     CDS             360..>788
                     /locus_tag="WGQ68_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0715824.1"
                     /translation="MSTFFRQTAQAMIAKHIDRFPLLKLDQVIDWQPIEQYLNRQKNR
                     YLRDHRGRPAYPLLSMFKAVLLGQWHSLSDPELEHSLITRIDFNLFCRFDELIIPDYS
                     TLCRYRNWLAQDDTLSELLKLINCQLTEKGLKVEKASAAVI"
BASE COUNT          209 a          221 c          151 g          207 t
ORIGIN      
        1 tgggtcagtt gcgtgtagct catggcaatc tttcttgcag gaaaggccgt atgctaccgc
       61 atactggcct ttttctgtta gggaaagttg cacttcaaat gcgaatccgc ctcctgatgt
      121 aatagtcgta gaagataatt gccctttatt cggaatattc ctaaaatcag gcctctgccc
      181 tgaatattgt tcatacaaat caaatatttc tcttcgactt acgttagaat atatcggcat
      241 tccatttttg ctggcagatg aatttggtct gatcgtttta aaaccaattt gtaagttgag
      301 acctttgcaa aatccccaaa atcccctaaa ttcccaccaa gccatttagg ggattttcca
      361 tgagcacctt cttccggcaa accgcacaag ccatgattgc caaacacatc gaccgtttcc
      421 cgctattgaa gttggaccag gtgattgatt ggcagccgat cgaacaatac ctgaaccgtc
      481 aaaaaaaccg ttaccttaga gaccaccgcg gccgtcccgc ctaccctctg ctgtccatgt
      541 tcaaagccgt cctgctcgga caatggcaca gcctctccga tcccgaactc gaacacagcc
      601 tcatcacccg catcgatttc aacctgtttt gccgtttcga cgaactgatc atccccgatt
      661 acagtacctt atgccgctac cgcaactggc tggcgcaaga cgacaccctg tccgaattac
      721 tcaaactgat taactgccaa ctgaccgaaa aaggcttaaa agtagagaaa gcatccgccg
      781 ccgtcatt
//
LOCUS       DAWFHY010000186          783 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.186, whole genome shotgun sequence.
ACCESSION   DAWFHY010000186 DAWFHY010000000
VERSION     DAWFHY010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.186"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            13..291
                     /locus_tag="WGQ68_002102"
     CDS             13..291
                     /locus_tag="WGQ68_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003691580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1132 family protein"
                     /protein_id="HEZ0715825.1"
                     /translation="MALYKYQPSSKYFGQSMALIAQKEFVNNVKEYDILESFSYFLNK
                     RVAHNIWKIYFSDESVIFIRKSEENGKTVHEFVYQEYTDSSDFNSMFE"
     gene            233..673
                     /locus_tag="WGQ68_002103"
     CDS             233..673
                     /locus_tag="WGQ68_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002244545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715826.1"
                     /translation="MNSFIKSTPIVLILILCSSNSYSEPVRLERSQIKFQSSNNLKSS
                     GFKLDSSSKSFAKFIEAANFEHIPTGAKARINAKITASVSRAGVLAGVGKLARLGAKF
                     STRAVPYVGTALLAHDVYETFKEDIQARGYQYDPETDKFAKVSG"
BASE COUNT          248 a          146 c          174 g          215 t
ORIGIN      
        1 actcaggcgc agttggcact ttataaatat cagccgtcta gcaagtattt tgggcaatcg
       61 atggcattaa ttgctcagaa agaatttgtt aataacgtaa aagagtacga tattttagag
      121 agtttctctt atttcttaaa taagagagtc gcacataata tttggaagat ttatttttct
      181 gatgaatctg ttatttttat aagaaaatca gaagagaacg gaaaaactgt tcatgaattc
      241 gtttatcaag agtacaccga tagttctgat tttaattcta tgttcgagta attcttattc
      301 tgaacctgta agattagaaa gatcacaaat taaatttcaa tcaagtaata atttaaaatc
      361 aagtggtttt aaattagatt caagttctaa gagttttgcc aaattcatag aagcggcaaa
      421 ttttgagcac atccctacgg gcgcaaaagc ccgaatcaac gccaaaataa ccgccagcgt
      481 ttcccgcgcc ggcgtattgg cgggggtcgg caaacttgcc cgcttaggcg cgaaattcag
      541 cacaagggcg gttccctatg tcggaacagc ccttttagcc cacgacgtat acgaaacttt
      601 caaagaagac atacaggcac gaggctacca atacgacccc gaaaccgaca aatttgcaaa
      661 ggtctcaggc taagtgcgcc tgttgccgcc taaaaggtaa cccggatgcc tgattatcgg
      721 gtatccgggg aggattaagg gggtatttgg gtagaattag ggagtgattg gtagcggaaa
      781 tag
//
LOCUS       DAWFHY010000187          749 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.187, whole genome shotgun sequence.
ACCESSION   DAWFHY010000187 DAWFHY010000000
VERSION     DAWFHY010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..749
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.187"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(1..179)
                     /locus_tag="WGQ68_002104"
                     /pseudo
     CDS             complement(1..179)
                     /locus_tag="WGQ68_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(216..702)
                     /locus_tag="WGQ68_002105"
                     /pseudo
     CDS             complement(216..702)
                     /locus_tag="WGQ68_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          197 a          190 c          171 g          191 t
ORIGIN      
        1 atcagtccga aataggctgc ccgcgcatag cggaacttac ggtgcagcgt accgaagctc
       61 tgttcgacca cataacgggt cttcgacaaa tatcggttac gtttggtttg cacttccgac
      121 agcggacggt tgcggcaggc tttgcgcata atgccgtcct gcagccgatg ctctttcagt
      181 tttccgtagg tcggattctt gaatccgaca ttactttaat cgtattcaat agaaaagtcc
      241 gcattgccgc caccccaatt atgcggataa ataccctgtt tgacataacg gtgaaacgta
      301 gaaaaccgcc aatcggaaat ttgtcctaca tagccatgtt tgaccggatt gaaatgcaga
      361 taatcaaaat ggcaggcaaa atcggcctca tcgcggatag tatgttccca aaagcgtttt
      421 tgccaaagcc tgagattgcc gccgattaaa tatttgctgt gccgcttgat ctgccgccag
      481 cgttccgaat aagcagaatc attgtccggc agccgccata tggtatgcag atggtcgggc
      541 attcaacacc catgccaaaa tttcaaacgg ataccgttcg cgcaccgcca ttaccgcctg
      601 ccgtaaagcc aaacgcaccg catcatcggt caaaatcttc tgccgtttat tggctacaac
      661 cgtaaaaaag taagtgccgc cattgcggta aaacgacggt atttcatagt attatgttcg
      721 gaatgatttt gtaggtcgga ttctcgaat
//
LOCUS       DAWFHY010000188          728 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.188, whole genome shotgun sequence.
ACCESSION   DAWFHY010000188 DAWFHY010000000
VERSION     DAWFHY010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.188"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..471
                     /locus_tag="WGQ68_002106"
     CDS             <1..471
                     /locus_tag="WGQ68_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEZ0715827.1"
                     /translation="NNKYSVNIKKGRENQYNREELKTENQENGTFHAASSLGLSAIYD
                     FKLNDKFDKFKPYIGARVAYGHVKHQVHSVETKTTIVTSKPNGGPVPSSVTKPPYHQS
                     HSISSLGLGVIAGVGFDITPKLTLDTGYRYHNWGRLENTRFKTHEVSLGVRYRF"
BASE COUNT          207 a          215 c          153 g          153 t
ORIGIN      
        1 aacaataaat attccgtcaa cataaaaaag gggagagaaa accagtacaa cagggaagaa
       61 ctgaagacgg aaaatcagga aaacggtaca ttccacgccg cttcttctct cggcttatcc
      121 gccatttacg atttcaaact caacgataaa ttcgataaat tcaaacccta tatcggtgcg
      181 cgcgtcgcct acgggcacgt taaacatcag gttcattcgg tggaaaccaa aaccacgatt
      241 gttacctcta aaccaaatgg aggccctgtc ccaagttcgg ttaccaaacc tccctatcac
      301 caaagccaca gtatcagcag cttgggtctt ggtgtcatcg ccggtgtcgg tttcgacatc
      361 acgcccaagc tgactttaga caccggatac cgctaccaca actggggacg cttggaaaac
      421 acccgcttca aaacccacga agtctcattg ggcgtgcgct accgcttctg attccccgat
      481 accgatgccg tctgaacctt cagacggcat ttttgattta cctgccgttt acaggcgcgg
      541 ggcgggcgtg gaaataccca aaccgtcatt cccgacaaca ccgtaatctt gaaacccgtc
      601 attcccgaca acaccgtaat cttgaaaccc gccattcccg acaataccgc aatctcgaaa
      661 ttcgtcattc ccgcgcaggc gggaatccag acccctgacg cggcgggaat ctatcggaaa
      721 tgactgaa
//
LOCUS       DAWFHY010000189          699 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.189, whole genome shotgun sequence.
ACCESSION   DAWFHY010000189 DAWFHY010000000
VERSION     DAWFHY010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..699
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.189"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(69..554)
                     /gene="fxsA"
                     /locus_tag="WGQ68_002107"
     CDS             complement(69..554)
                     /gene="fxsA"
                     /locus_tag="WGQ68_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009349087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HEZ0715828.1"
                     /translation="MRFFGIGFLVLLFLEIMSIVWVADWLGGGWTLFLMAATFAAGVL
                     MLRHTGLSGLLLAGAAMRSGGRVSVYQMLWPIRYTVAAVCLMSPGFVSSVLAVLLLLP
                     FKGGAVLQAGGAENFFNMNQSGRKEGFSRDDDIIEGEYTVEEPYGGNRSRNAIEHKKD
                     E"
BASE COUNT          210 a          215 c          127 g          147 t
ORIGIN      
        1 tgttaatcca ctatattttc aaaccggaaa aatgccgtct gaatcttcag acggcattcc
       61 attcatattt attcgtcttt tttgtgttcg atggcgtttc gggaacgatt gccgccgtaa
      121 ggctcttcaa ccgtatattc cccctcgata atatcgtcat cgcgggaaaa gccctctttt
      181 ctgcccgatt ggttcatgtt gaaaaaattt tccgcacctc ctgcctgcaa cactgcacct
      241 cccttaaacg gcagcagcag caataccgcc aacaccgagg atacgaatcc cggactcatc
      301 agacacaccg ccgccaccgt ataacggata ggccacaaca tctgataaac ggataccctc
      361 ccgccgcttc tcattgccgc gcccgccaat aaaagaccgg acagccccgt atgcctgagc
      421 atcagcacgc cggcggcaaa ggttgccgcc attagaaaca gcgtccaacc gccgcccaac
      481 caatcggcaa cccacacaat cgacataatc tccaaaaaca gcagcaccaa aaaaccgata
      541 ccgaaaaatc tcattgaccg tcatccttat atttaagtaa acagcaaacc gcccgaacag
      601 gactccaagc gagctgcctg taaatgatta caaaaccatg tgcttcaagc cgaaacaatg
      661 tgaaatcacg caatatagtg gattaacaaa aaccagtac
//
LOCUS       DAWFHY010000190          693 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.190, whole genome shotgun sequence.
ACCESSION   DAWFHY010000190 DAWFHY010000000
VERSION     DAWFHY010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..693
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.190"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(<1..563)
                     /locus_tag="WGQ68_002108"
     CDS             complement(<1..563)
                     /locus_tag="WGQ68_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002237266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HEZ0715829.1"
                     /translation="MTKLYAEIAKREAQDDGTVKVLGYASSEAVDSDGEVIATAAMKA
                     AIPDYMKFDAGREMHGSNAAGTAIEINVEDDGITFFGAHIIDPVVVSKVKTGVYKGFS
                     IGGSVTARDNLNKSQITGLKLTEISLVERPANPDAVFTCFKADKGAEAVNNDTEHNAT
                     YFSHFPSKQKSTGGSRCPFLYRFPYFFT"
BASE COUNT          154 a          182 c          171 g          186 t
ORIGIN      
        1 gtgaaaaaat aggggaacct gtaaaggaag gggcatcggc tgccgccggt gcttttttgt
       61 ttggaaggga aatggctaaa atatgtagca ttatgttctg tatcgttgtt taccgcttcc
      121 gcacctttgt ccgccttaaa gcaggtaaac accgcatcgg gattggcagg gcgctcgaca
      181 aggctgattt ccgtcagctt caagcccgtg atttgcgact tgttcaaatt atcgcgggcg
      241 gtaacgctgc cgccgatgga aaagcctttg taaacgcctg ttttgacttt cgacacgaca
      301 acggggtcga tgatatgagc accgaaaaag gttatgccgt cgtcttcaac gttgatttca
      361 attgccgttc ccgcagcgtt tgagccgtgc atctcgcgcc ccgcgtcaaa cttcatataa
      421 tcgggaatcg ccgccttcat agctgccgtc gcgatgactt caccgtccga atcgaccgcc
      481 tcgcttgagg cataacccaa aaccttgacc gtgccgtcgt cctgcgcctc cctcttggcg
      541 atttctgcgt ataactttgt catgggttta ctcctgcctg tataaatggc atgctgaatt
      601 taaagccctt ttgtaaggct ggaagtgtac ttgccttaac gctatccgct ccagaacgac
      661 actgcaaaag ggctttactc atccttcaag atg
//
LOCUS       DAWFHY010000191          691 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.191, whole genome shotgun sequence.
ACCESSION   DAWFHY010000191 DAWFHY010000000
VERSION     DAWFHY010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.191"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(1..121)
                     /locus_tag="WGQ68_002109"
                     /pseudo
     CDS             complement(1..121)
                     /locus_tag="WGQ68_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(158..649)
                     /locus_tag="WGQ68_002110"
     CDS             complement(158..649)
                     /locus_tag="WGQ68_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0715830.1"
                     /translation="MKYRRFYRNGGTYFFTVVTNKRQKILTDDAVRLALRQAVMAVRE
                     RYPFEILAWVLMPDHLHTIWRLPDNDSAYSERWRQIKRHSQYLIGGNLRLWQKRFWEY
                     TIRDEADFAWHFDYLHFNPVKHGYVGQISDWGFSTFHRYVKQGIYPHNWGGGNADFSI
                     GYD"
BASE COUNT          185 a          173 c          151 g          182 t
ORIGIN      
        1 aatttaatcc actataacgg gttttcgaca aatatcggtt gcgtttggtt tgcgcctccg
       61 tcagcggacg gttgcggcag gctttgcgca taatgccgtt ctgcaaccga tgctctttca
      121 gttttccgta ggtcggattc tcgaatccga cattacttca atcgtatcca atagaaaagt
      181 ccgcattgcc gccaccccaa ttatgcggat aaataccctg tttgacataa cggtgaaacg
      241 tagaaaaccc ccaatcggaa atttgtccta catagccatg tttgaccgga ttgaaatgca
      301 gataatcaaa atgccaggca aaatcggcct catcgcggat agtatattcc caaaagcgtt
      361 tttgccaaag cctgagattg ccgccgatta aatattggct gtgccgcttg atttgccgcc
      421 agcgttccga ataagcagaa tcattgtccg gcagccgcca tatggtatgc agatggtcgg
      481 gcatcaacac ccatgccaaa atttcaaacg gataccgttc gcgcaccgcc attaccgcct
      541 gccgtaaagc caaacgcacc gcatcatcgg tcaaaatctt ctgccgttta ttggttacaa
      601 ccgtaaaaaa gtaagtgccg ccattgcggt aaaaacgacg gtatttcata gtattatgct
      661 cggaatgatt ttgtaggtcg gattcttgaa t
//
LOCUS       DAWFHY010000192          671 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.192, whole genome shotgun sequence.
ACCESSION   DAWFHY010000192 DAWFHY010000000
VERSION     DAWFHY010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.192"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          202 a          140 c          142 g          187 t
ORIGIN      
        1 gccgccttgt cctgattttt gttaatccga gacctttgca aaaatagtct gttaacgaaa
       61 tttgacgcat aaaaatgcgc caaaaaattt tcaattgcct aaaaccttcc taatattgag
      121 caaaaagtag gaaaaatcag aaaagctttg cattttgaaa atgagattga gcataaaatt
      181 ttagtaacct atgttattgc aaaggtctca atccactata aagaccgtcg ggcatctgca
      241 gccgtcattc ccgcgcaggc gggaatctag tccgttcggt ttcggttttt tggctagtgc
      301 cgcaacatta aatttctaga ttcccacttt cgtgggaatg acgcgattag agtttcaaaa
      361 tttattctaa atagctgaaa ctcaacgcat tggattcccg cctgcgcggg aatgacgaat
      421 ttcaggttgc tgtttttggt tttctgtttt tgtgaaaata acgggatttc agcttgtggg
      481 tatttactgg aaaaaacaga aaccgctccg ccgtcattcc cgcgcaggcg ggaatccaga
      541 cattcaatgc taaggcaatt tatcgggaat gactgaaact caaaaagctg gattcccact
      601 ttcgtgggaa tgacgaagtg gaagttaccc gaaacttaaa acaagcgaaa ccgaacgaac
      661 cggattccca c
//
LOCUS       DAWFHY010000193          667 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.193, whole genome shotgun sequence.
ACCESSION   DAWFHY010000193 DAWFHY010000000
VERSION     DAWFHY010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.193"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>667
                     /locus_tag="WGQ68_002111"
     CDS             <1..>667
                     /locus_tag="WGQ68_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0715831.1"
                     /translation="LGQWHSLSDPELEHSLITRIDFNLFCRFDELSIPDYSTLCRYRN
                     RLAQDDTLSELLELINRQLTEKGLKIEKASAAVIDATIIQTAGSKQRQAIEVDEEGQV
                     SGQTTPSKDKDARWTKKNGLYRLGYKQHTRTDEEGYIKKLHITPANAHECKHLLPLLE
                     GLPKGTTVYADKGYDSEENRQHLEEHQLLDGIMRKAHRKHPLSEVQTKRNRYLSKTRY
                     VVE"
BASE COUNT          206 a          189 c          150 g          122 t
ORIGIN      
        1 tgctcggaca atggcacagt ctctccgatc ccgaactcga acacagcctc atcacccgca
       61 tcgatttcaa cctgttttgc cgtttcgacg aactgagcat ccccgattac agcaccttat
      121 gccgctaccg caaccggctg gcgcaagacg acaccctgtc cgaattgctg gaactgatta
      181 accgccaact gaccgaaaaa ggtttaaaaa tagagaaagc atccgccgcc gtcattgatg
      241 ccaccattat tcagactgcc ggcagcaaac agcgccaggc catagaagtc gacgaggaag
      301 gacaagtcag cggccaaacc acaccgagta aagataaaga tgcccgttgg acaaagaaaa
      361 acggtttata cagactcggt tacaaacaac atacccgtac cgatgaggaa ggctatatca
      421 agaaactgca catcaccccc gccaatgccc atgagtgcaa acacctgttg cctttgttgg
      481 aagggttacc caaaggtacg accgtctatg ccgataaagg ctacgacagt gaggaaaacc
      541 ggcaacatct ggaagagcat cagttgttag acggtattat gcgcaaagcc caccgcaaac
      601 atccactgtc ggaagtgcaa accaaacgca accgatattt gtcgaagacc cgttatgtgg
      661 tcgaaca
//
LOCUS       DAWFHY010000194          620 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.194, whole genome shotgun sequence.
ACCESSION   DAWFHY010000194 DAWFHY010000000
VERSION     DAWFHY010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.194"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          177 a          135 c          163 g          145 t
ORIGIN      
        1 agaccttaga acaacagcaa tattcaaagg ttagctgaag ctttagagat tctggattcc
       61 cactttcgtg ggaatgacgg gatttgagat tgcggcattt atcggaaaaa acagcaaccg
      121 ctccgccgtc attcccgcgc aggcgggaat ctagaccttg ggataacggc aatattcaaa
      181 ggttataaaa gacccgtcat tcccgcgcag gcgggaatct agaccttaga acaacagtaa
      241 tattcaaagg ttagctgaag ctttagagat tctagattcc cactttcgcg ggaatgacgg
      301 aatgtaggtt cgtgggaatg acgcggtgca ggtttccgtg cggatggatt cgtcattccc
      361 gcgcaggcgg gaatccagac cttagaacaa cagtaatatt caaagattat aaaagacccg
      421 tcattcccgc gcaggcggga atccagacct tagaacaaca gtaatattca aaggttagct
      481 gaagctttag agattctgga ttcccacttt cgtgggaatg acgggatgta ggttcgtggg
      541 aatgacggga tttgagattg cggcatttat cggaaaaaac agaaaccgct ccgccgtcat
      601 tcccgcgcag gcgggaatct
//
LOCUS       DAWFHY010000195          560 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.195, whole genome shotgun sequence.
ACCESSION   DAWFHY010000195 DAWFHY010000000
VERSION     DAWFHY010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.195"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..186
                     /locus_tag="WGQ68_002112"
     CDS             <1..186
                     /locus_tag="WGQ68_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715832.1"
                     /translation="MSEDWIKQVITNLPDNHPTKNILREAVRSGKIKTAVTGVDRQTG
                     KAVILPVKVPSKTNIRR"
     gene            189..>560
                     /locus_tag="WGQ68_002113"
     CDS             189..>560
                     /locus_tag="WGQ68_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ0715833.1"
                     /translation="MGHNMMTTQKWYEHITDVIIDTTQRFNREKSADSIEYINERKGS
                     PIGAICVVSIDVYAACSHAYLFEHNLKKFKQYAYAYSKLEILASMGWSDPTPFFFPCD
                     MLNIQNPMFLMLMSDSPQLREF"
BASE COUNT          185 a          108 c          113 g          154 t
ORIGIN      
        1 atgagtgagg attggattaa acaagttatt actaacttac cagataatca tcccactaaa
       61 aacatccttc gagaagcagt acgttcgggt aaaatcaaaa ctgcggttac tggagtagat
      121 cgtcaaacag gtaaagcagt tattcttcct gttaaagttc cttctaaaac caatataagg
      181 agataacaat ggggcacaat atgatgacca cccaaaaatg gtatgaacat attacagatg
      241 tgattataga caccactcaa agatttaata gagagaaaag cgctgatagc atagagtata
      301 ttaatgaaag aaagggatct ccaattggag cgatctgtgt cgtttctata gatgtttatg
      361 cggcatgctc acatgcttat ctgtttgaac acaaccttaa aaagttcaaa cagtatgctt
      421 atgcttacag taaacttgag atactggcaa gtatgggatg gtctgacccc acgcctttct
      481 tctttccctg tgacatgctc aacattcaaa atccgatgtt tctgatgctg atgagcgaca
      541 gcccgcagct gcgcgagttt
//
LOCUS       DAWFHY010000196          557 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.196, whole genome shotgun sequence.
ACCESSION   DAWFHY010000196 DAWFHY010000000
VERSION     DAWFHY010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..557
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.196"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..186
                     /locus_tag="WGQ68_002114"
     CDS             <1..186
                     /locus_tag="WGQ68_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715834.1"
                     /translation="MSREWIKQVVKSLPDGSPAKAVVLKANQNGKLKTAIAGVDRQTG
                     KAVILSVKVPSKTNIRR"
     gene            189..>557
                     /locus_tag="WGQ68_002115"
     CDS             189..>557
                     /locus_tag="WGQ68_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
                     /protein_id="HEZ0715835.1"
                     /translation="MGHNMMTTQKWYEHITNVIIGNTANFNSGCPESIDYVDEKKGVP
                     LAAMKYILMYTEAAASHAYLFEHDLKKFKQYAYVAGKLGILQSVDDEDPEPFFFPCDM
                     LNIQDPMFLMLMSDSPQLREF"
BASE COUNT          179 a          107 c          119 g          152 t
ORIGIN      
        1 atgagtcgtg aatggattaa acaagttgta aaaagtttac ctgatggtag tcctgctaag
       61 gcagttgtct taaaagcaaa tcagaacggc aaattaaaaa cggcaatagc aggcgttgat
      121 cgtcaaacag gtaaggccgt tattctttct gtcaaagttc cttctaaaac caatataagg
      181 agataacaat ggggcacaat atgatgacca cccaaaaatg gtatgaacat attactaatg
      241 taatcatagg caatactgct aatttcaata gcggttgccc cgaatctata gattatgtag
      301 atgaaaaaaa aggcgtgccg cttgcagcga tgaaatacat tttaatgtac actgaagctg
      361 cggcttccca tgcctatcta tttgaacatg atcttaagaa attcaagcaa tatgcttatg
      421 ttgcaggaaa gttgggtatt ttgcagagtg tagatgatga agaccccgaa cccttcttct
      481 ttccctgcga catgctcaac attcaagatc cgatgtttct gatgctgatg agcgacagcc
      541 cgcagctgcg cgagttt
//
LOCUS       DAWFHY010000197          541 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.197, whole genome shotgun sequence.
ACCESSION   DAWFHY010000197 DAWFHY010000000
VERSION     DAWFHY010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..541
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.197"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            330..>541
                     /locus_tag="WGQ68_002116"
     CDS             330..>541
                     /locus_tag="WGQ68_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEZ0715836.1"
                     /translation="MSTFFQQTAQAMIAKHINRFPLLKLDQVIDWQPIEPYLNRQKTR
                     YLRDHRGRPAYPLLSMFKAVLLGQWH"
BASE COUNT          152 a          145 c           97 g          147 t
ORIGIN      
        1 aaaaatattt tctttcaaac tcaacttgta tttgtcggga aacacgataa ttccttaaat
       61 aattaaagca gtacgttaaa tcatcatctt atcatcactt tcggggttta tgttgcgtgc
      121 gtccgttttc taatacggtt tcccatcccg cgaaacgagg cggagacctg tgcaaaaaac
      181 ctgttcacga aattttccgc attaacgata cgctgaaaat gcgctaaaaa tgactgtgtt
      241 tgaatatcgg ttgatttcat ccgtttgaga cctttgcaaa attccccaaa atcccctaaa
      301 ttcccaccaa gacatttagg ggattttcca tgagcacctt cttccagcaa accgcccaag
      361 ccatgattgc caaacacatc aaccgtttcc cgctattgaa gttggaccaa gtgattgatt
      421 ggcagccgat cgaaccatac ctgaaccgtc aaaaaacccg ttacctccga gaccaccgcg
      481 gtcgtcccgc ctatcccctg ttgtccatgt tcaaagccgt cctgctcgga caatggcaca
      541 g
//
LOCUS       DAWFHY010000198          532 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.198, whole genome shotgun sequence.
ACCESSION   DAWFHY010000198 DAWFHY010000000
VERSION     DAWFHY010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.198"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>532
                     /locus_tag="WGQ68_002117"
     CDS             <1..>532
                     /locus_tag="WGQ68_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002242184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEZ0715837.1"
                     /translation="RSKPHVNVGTIGHVDHGKTTLTAALTTILAKKFGGAAKAYDQID
                     NAPEEKARGITINTSHVEYETETRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAA
                     DGPMPQTREHILLARQVGVPYIIVFMNKCDMVDDAELLELVEMEIRDLLSSYDFPGDD
                     CPIVQGSALKALEGDAA"
BASE COUNT          144 a          162 c          128 g           98 t
ORIGIN      
        1 acgtagcaaa ccgcacgtaa acgttggcac catcggtcac gttgaccatg gtaaaaccac
       61 cctgactgcc gctttgacta ctattttggc taaaaaattc ggcggtgctg caaaagctta
      121 cgaccaaatc gacaacgcac ccgaagaaaa agcacgcggt attaccatta acacctcgca
      181 cgtggaatac gaaaccgaaa cccgccacta cgcacacgta gactgcccgg ggcacgccga
      241 ctacgttaaa aacatgatta ccggcgccgc acaaatggac ggtgcaatcc tggtatgttc
      301 cgcagccgac ggccctatgc cgcaaacccg cgaacacatc ctgctggccc gccaagtagg
      361 cgtaccttac atcatcgtgt tcatgaacaa atgcgacatg gtcgacgatg ccgagctgtt
      421 ggaactggtt gaaatggaaa tccgcgacct gctgtccagc tacgacttcc ccggcgacga
      481 ctgcccgatt gtacaaggtt ccgcactgaa agccttggaa ggcgatgccg ct
//
LOCUS       DAWFHY010000199          532 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.199, whole genome shotgun sequence.
ACCESSION   DAWFHY010000199 DAWFHY010000000
VERSION     DAWFHY010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.199"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(114..386)
                     /locus_tag="WGQ68_002118"
     CDS             complement(114..386)
                     /locus_tag="WGQ68_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002220648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715838.1"
                     /translation="MFAVIFFSTLGCILAWIRDIPKIKSKKILARSLYVIGIINVIIS
                     YVLIKNILVSVSDGGGIKYVAIYLSNLFFWTVLMYVLVKRLSKKPS"
BASE COUNT          175 a          106 c           73 g          178 t
ORIGIN      
        1 cgtatgttgc acacccaatt tcaataggta aatcagattg caaatccagc aatttgaata
       61 ttgtcattgt tccgtgcaaa agggattttt attgatgagt tgtgtactgg gtttcagctt
      121 ggctttttag ataatctttt cacaagaaca tacatcaata ctgtccaaaa aaaaagattt
      181 gataaatata ttgcaacata ttttattcca cctccatccg aaacagaaac caatatattt
      241 ttaattaata catagctgat tattacattt attattccta taacatataa ggatcttgcc
      301 aaaatttttt ttgattttat tttaggaata tctcttatcc atgctaaaat acagcccaat
      361 gtcgaaaaga aaataacagc aaacactacc ttacctcctc tcacttccaa ccaaaccgat
      421 agcaggtttg gttggaagtt ggtttattta ttatttaacg gcgaatgtca gtgttcttac
      481 ccgtagaacc tgcataacca tttatgccac cagcaacagt ccccactgct ac
//
LOCUS       DAWFHY010000200          529 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.200, whole genome shotgun sequence.
ACCESSION   DAWFHY010000200 DAWFHY010000000
VERSION     DAWFHY010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..529
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.200"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            245..529
                     /locus_tag="WGQ68_002119"
     CDS             245..529
                     /locus_tag="WGQ68_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002246543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEZ0715839.1"
                     /translation="MQPAVYILASQRNGTLYIGVTSDLVQRIYQHREHLIEGFTSRYN
                     VTMLVWYELHPTMESAITREKQLKKWNRAWKLQLIEENNVSWQDLWFDII"
BASE COUNT          158 a           83 c          137 g          151 t
ORIGIN      
        1 tttatcggaa aaaacagaaa ccgctccgcc gtcattcccg cgcaggcggg aatctaggtt
       61 tgtcggtgcg gaaacttatc gggtaaaacg gtttctttag attttgcgtt ctagattccc
      121 actttcgcgg gaatgacgaa gagttgcggg aatgatggaa agctatggga ataacgaagg
      181 gttaaagtaa tcacgggatg gtgttcgcgg gaatataaat tgaaataatt caaaagggta
      241 ttatatgcag cctgcggttt atattttagc aagccaacgt aatggcacgt tatacattgg
      301 cgttacatct gatttggtgc aacgtattta ccaacatagg gaacatttga ttgagggatt
      361 tacatcacgg tacaacgtta ctatgctggt ttggtatgaa ctgcatccta cgatggagag
      421 cgcaattact cgggaaaaac agttgaagaa atggaacagg gcttggaaat tgcaactgat
      481 tgaagaaaat aatgtttctt ggcaggattt atggtttgat attatttag
//
LOCUS       DAWFHY010000201          522 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.201, whole genome shotgun sequence.
ACCESSION   DAWFHY010000201 DAWFHY010000000
VERSION     DAWFHY010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.201"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(1..72)
                     /locus_tag="WGQ68_002120"
                     /pseudo
     CDS             complement(1..72)
                     /locus_tag="WGQ68_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
BASE COUNT          109 a          103 c          143 g          167 t
ORIGIN      
        1 gggccatttg ccgtgattca ggtaatactc ggtgacggct gatttttgac cttcggccaa
       61 aaggatggat tcgtcattcc cgcgcaggcg ggaatccagg tttgtcggca cggaaactta
      121 tcgggaaaac ggtttcttga gattttgcgt tctggattcc cactttcgtg ggaatgacgg
      181 gattaaagtt tcaaaattta ttctaaataa ctgaaactca acgaactaga ttcccgcttt
      241 tgcgggaatg ccgaatttta ggtttctgtt tttggttttc tgtctttgtg ggaatgatga
      301 aattttaagt tttaggaatt tatcggaaaa aacagaaacc gctccgccat cattcccgcg
      361 caggcgggaa tccagcctcg tcggtgcgga aacttatcgg gtaaaaaggt ttctctagtt
      421 tggtgtcgat tttcttgtcg gtgctgttga cggcaggtgc ttggtgtcga ttttgtcgtt
      481 gccgttgccg gtgactttgt taacggcgtc ggcgctggcg tt
//
LOCUS       DAWFHY010000202          521 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.202, whole genome shotgun sequence.
ACCESSION   DAWFHY010000202 DAWFHY010000000
VERSION     DAWFHY010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..521
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.202"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(358..>521)
                     /locus_tag="WGQ68_002121"
     CDS             complement(358..>521)
                     /locus_tag="WGQ68_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HEZ0715840.1"
                     /translation="AKRQDGSVKWFCGQPVTRDAKATNDDVTADNGTDKKIDTKHLPS
                     TCRDASSAS"
BASE COUNT          114 a          128 c          143 g          136 t
ORIGIN      
        1 ccgataagtt tccgcaccga caaaactaga ttcccacttt cgtgggaatg acgtgctgca
       61 ggtttccgta tggatggatt cgtcattccc gagtaggcgg gaatccagtc cgcttgttcg
      121 gtaaatgaga gggcggattg cgcgcctgtc agataaaccc cgtgtttaaa cgggcggcaa
      181 tgaggtacac gcagagcctt gaagcgcaat cgatatatta tttccaccaa aacggaaacg
      241 gacgcccccg cttgccttgc aaacctttaa aaatgaagcc acccggatta atccgagtgg
      301 ccgtggaaaa tcacttaccg cttgatttat ttaaaattta tggtataatt tgccttgtta
      361 gctggcagat gatgcgtcgc ggcaggttga cggcaggtgc ttggtgtcga ttttcttgtc
      421 ggtgccgttg tcggcggtaa cgtcgtcgtt ggtggctttg gcgtcgcgcg taaccggctg
      481 tccgcagaac cattttaccg aaccgtcttg acgcttggcc c
//
LOCUS       DAWFHY010000203          504 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.203, whole genome shotgun sequence.
ACCESSION   DAWFHY010000203 DAWFHY010000000
VERSION     DAWFHY010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..504
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.203"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>504
                     /locus_tag="WGQ68_002122"
     CDS             <1..>504
                     /locus_tag="WGQ68_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002219733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="filamentous hemagglutinin N-terminal
                     domain-containing protein"
                     /protein_id="HEZ0715841.1"
                     /translation="RVVVNQINSSHSSQMNGYIEVGGRRAEVVIANPAGIAVNGGGFI
                     NASRATLTTGQPQYQAGALTGFQVRSGHIAITGQGLDARDTDFTQILSHAVKIDGPVW
                     GKDVRVSAGKNNVSADGSIRSSHSPATNINSDNSLYAIDTGALGGMYAGKITLISTDR
                     DASVRNQG"
BASE COUNT          125 a          113 c          139 g          127 t
ORIGIN      
        1 acgtgtggtt gtaaaccaaa tcaacagcag ccattcttca caaatgaatg gctatattga
       61 ggtgggcgga cgacgtgcag aagtcgttat tgccaatccg gcagggattg cagtcaatgg
      121 tggtggtttt atcaatgctt cccgtgccac tttgacgaca ggccaaccgc aatatcaagc
      181 aggcgcatta acaggctttc aggttcgttc gggtcatatt gcgatcacgg gacaaggttt
      241 ggatgcacgt gataccgatt tcacgcagat attgagccat gcggttaaga ttgatgggcc
      301 tgtgtggggt aaagatgtgc gtgtgtcggc agggaaaaat aatgtctcag ctgatggctc
      361 tatccggtct tcacactccc ctgctacgaa tataaattcc gacaactcac tgtatgccat
      421 cgatacaggg gcattgggcg gtatgtatgc cggtaagatc accctgatca gcactgatcg
      481 tgatgcatcc gtgcgcaatc aggg
//
LOCUS       DAWFHY010000204          501 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.204, whole genome shotgun sequence.
ACCESSION   DAWFHY010000204 DAWFHY010000000
VERSION     DAWFHY010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.204"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(124..402)
                     /locus_tag="WGQ68_002123"
     CDS             complement(124..402)
                     /locus_tag="WGQ68_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002223477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715842.1"
                     /translation="MYYQVGNKCLEKHQAENLYFSLVVPRIKENGQIVRPEYNGSLWK
                     MSDGQPLRLLLAECSLKDNLQSGLETGWIVFGILASVYFVSLLKKVLK"
BASE COUNT          139 a          132 c           94 g          136 t
ORIGIN      
        1 ctgcctttca atgattgttt tgaaagcctg attttgacac cataacttca tgcgctcaat
       61 tcttaaacag aaccgccccg attaatacgg gtacggaaac gccgagataa aaataaaaat
      121 ccatcatttc aaaacctttt tcagcaggga aacaaagtaa acggacgcga ggatgccgaa
      181 tactatccag cctgtttcaa ggccgctttg caggttgtct ttcagactgc attccgccaa
      241 taaaagcctt agcggctgac cgtccgacat cttccacagg ctgccgttat attccggcct
      301 gacaatctgt ccgttttctt tgattcttgg tactaccaag ctgaaataaa ggttttcagc
      361 ctggtgcttc tcaagacatt tatttccgac ttggtagtac atgccgtctt acttcatcac
      421 tctcttaacg atggaaaata caaaaagcgc ggcgaaaacg ccaacgacaa tccaaccggc
      481 ttccataccg tccgcttttg c
//
LOCUS       DAWFHY010000205          501 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.205, whole genome shotgun sequence.
ACCESSION   DAWFHY010000205 DAWFHY010000000
VERSION     DAWFHY010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.205"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..330
                     /locus_tag="WGQ68_002124"
     CDS             <1..330
                     /locus_tag="WGQ68_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003702963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0715843.1"
                     /translation="DCPYGGSNNLEKAKLRAERLSKNDRAGKDFTKAGKEAVIDLNRI
                     QNNGQVKCANCGIETIPAKQSIKNISPTSNERQVDHVIPKSKGGQGTPKNGQVLCRGC
                     NIKKSNK"
BASE COUNT          183 a           83 c          104 g          131 t
ORIGIN      
        1 gattgtccgt atggcggttc aaataattta gagaaagcaa aacttcgcgc agaacggctt
       61 agtaaaaacg atcgggcagg aaaagacttt actaaagcag gaaaagaagc cgttattgat
      121 ttgaatagga ttcaaaataa tggtcaagtt aagtgcgcta attgcggtat tgaaacaatt
      181 ccagccaaac aatctataaa aaatatttct cctacttcaa atgaaagaca agtagatcat
      241 gtcatcccta aatctaaggg tggtcaaggt acacctaaaa acgggcaggt attatgcaga
      301 ggctgcaaca ttaagaagag taacaaatga aatcgaaact tactgtcgtc tattacgatt
      361 tggaaagcaa tattgcagaa gaaatactgt caggaaacat aatgcctgat ggaaattttc
      421 ttattcaaga gattcctctt ttcgcaccga atttggcttt aaacgacatt gttgccatag
      481 aacgtgagga taagatgctg t
//
LOCUS       DAWFHY010000206          497 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.206, whole genome shotgun sequence.
ACCESSION   DAWFHY010000206 DAWFHY010000000
VERSION     DAWFHY010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..497
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.206"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          141 a          134 c          129 g           93 t
ORIGIN      
        1 agttgcaaga atgacggagt ttcaggcggc atccgaccgc cccgtcattc ccgcgaaagc
       61 gggaatctaa aaacccaacg ctgcaagatt tatcagaaac aactgaaacc gaacggactg
      121 gattcccgcc tgcgcgggaa tgacgggatt ttagtaaccg tagcaaccgc ctgcgcgacg
      181 gctaaggggc ttcagcaacc gtagcaactg cctgtgtggg aatgacggac aatgggcttc
      241 agacggcatc tcttgcctgc cgctaaaaca gtttgccgca caactgttca aacgcgtccg
      301 atatgtttca acacacagga cgacacataa agcaccgccc tatgtgtcgt cctgatttgg
      361 aaggggttac acccccctcc caaataaagt ctgatcctgc cgccccgaag gacagatgtc
      421 cgagtggcgg ggtttcaacc gaaaaggaaa tacgatgaag tggtacaatt agcggcaatg
      481 cggacagaca aattaaa
//
LOCUS       DAWFHY010000207          495 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.207, whole genome shotgun sequence.
ACCESSION   DAWFHY010000207 DAWFHY010000000
VERSION     DAWFHY010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 495)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 495)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..495
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.207"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          152 a          113 c          113 g          117 t
ORIGIN      
        1 atttcaatgc ctcaagaatt tatcggaaaa aaacaaaacc cttccgtcgt cattcccacg
       61 aaagtgggaa tctagaaatg aaaagcagca ggaatttatc ggaaatgacc ggaactgaac
      121 ggactggatt cccgctcagg cgggaatcta gaatttcaat gcctcaagaa tttatcggaa
      181 aaaaccaaaa cccttccgcc gtcattccca cgaaagtggg aatctagaaa tgaaaagcaa
      241 caggaattta tcggaaatga ccgaaactga acggactgga ttcccgcttt tgcgggaatg
      301 acggcgacaa ggttgctgtt atagcggatt aacaaaaacc agtacggcgt tgcctcgcct
      361 tagctcaaag agaacgattc tctaaggtgc tgaagcacca agtgaatcgg ttccgtacta
      421 tttgtactgc ctgcggcttc gtcgtcttgt cctgattttt gttaatccgc tataaagacc
      481 gtcgggcatc tgcag
//
LOCUS       DAWFHY010000208          477 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.208, whole genome shotgun sequence.
ACCESSION   DAWFHY010000208 DAWFHY010000000
VERSION     DAWFHY010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.208"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            65..>477
                     /locus_tag="WGQ68_002125"
     CDS             65..>477
                     /locus_tag="WGQ68_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002257668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer family protein"
                     /protein_id="HEZ0715844.1"
                     /translation="MLGVVPGIGESIQAYKVAKAAKNLQGMKKALDKAATVATAQGYV
                     SKTKIKIGQTELRVTAATDKQLLKTISEGRDTTGKMTEQLFDSLAKQNGFRVLSGGKY
                     GGNNGFDHVWQAADGSVVLIVESKQIRNGTVQLNP"
BASE COUNT          153 a           91 c          123 g          110 t
ORIGIN      
        1 tccaattatc ggtgatatac agagttttgt acaagcacag accgctgccg atcacctgtt
       61 tgctttgctg ggtgtggttc cgggtatcgg tgaatcgata caggcctata aagtagcgaa
      121 agcggcaaaa aatttacaag gcatgaaaaa agccttggac aaggcagcaa ccgttgccac
      181 tgcacagggc tatgtcagca aaaccaaaat caaaatcggt caaactgaat taagggttac
      241 tgcagcaact gacaaacaat tgctgaaaac cattagcgaa ggaagggaca cgacaggtaa
      301 aatgaccgag cagttatttg actctttagc taaacaaaat ggcttcagag tgctttcggg
      361 cggcaaatac ggcggaaata acggttttga tcatgtatgg caggctgctg atggtagtgt
      421 cgttttgatt gtagaaagta agcagattag gaacggtacg gtacagctga atccgaa
//
LOCUS       DAWFHY010000209          455 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.209, whole genome shotgun sequence.
ACCESSION   DAWFHY010000209 DAWFHY010000000
VERSION     DAWFHY010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.209"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(121..>455)
                     /locus_tag="WGQ68_002126"
     CDS             complement(121..>455)
                     /locus_tag="WGQ68_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HEZ0715845.1"
                     /translation="VDKNSDTMHFKGFKAADVHFIRSGSDLVLSASEQDNVRISGFFY
                     GENHRVDTFVFDDAAISNPDFAKYINAGNNLVQSMSVFGSNTAATGGNVDANTQSVQQ
                     PLLVTPSA"
BASE COUNT          150 a           93 c           82 g          130 t
ORIGIN      
        1 agaagtgcac cgattaaggt taattaaaat acgaccctta tcccaatatt aagaaaaaca
       61 gcaatcaaaa taaacttgat tgctgttttt cagtttaagc catgaatgtg attaggctcc
      121 ttatgcagat ggcgttacca ataacggctg ctgtacggat tgtgtattgg catccacatt
      181 tcctcctgtc gcagcagtat tagaaccgaa cacagacata gactgtacca aattattgcc
      241 agcattaata tacttggcaa aatctggatt actgatagct gcatcatcaa agacaaatgt
      301 atctacacga tggttttcac catagaagaa tccggaaata cgtacgttgt cttgttcaga
      361 agcgctaagc accaaatcac ttccggaacg gataaaatga acatctgctg ctttaaatcc
      421 tttaaagtgc atagtgtcag agtttttatc cacat
//
LOCUS       DAWFHY010000210          441 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.210, whole genome shotgun sequence.
ACCESSION   DAWFHY010000210 DAWFHY010000000
VERSION     DAWFHY010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.210"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          145 a           97 c           75 g          124 t
ORIGIN      
        1 aatattagga aggttttagg caattgaaaa ttttttggcg catttttatg cgtcaaattt
       61 cgttaacaga ctatttttgc aaaggtctca tgcagatatt cgaatatcga atttgcctta
      121 tgattacggt tttctgtctt atctgcctta aaacgccgat attccgtttg aaccaaatta
      181 tatttgccga aacgctcaaa aatctccctt acctgcccga cagacatcaa gccttcgttg
      241 ttgtagctga gaaaaatata ttgaaagtcc gcatccctaa tcaaagcccc aaataccgga
      301 accacatccg attttgagca agtccgcgct aaaacacatc gtacagcgca aaaccggatg
      361 ggaatggcaa aatccggaaa caaacatttt tcaaagcata caaacaaaaa aataccaacc
      421 ggaggttggt attccttaac t
//
LOCUS       DAWFHY010000211          436 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.211, whole genome shotgun sequence.
ACCESSION   DAWFHY010000211 DAWFHY010000000
VERSION     DAWFHY010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..436
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.211"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            27..>436
                     /locus_tag="WGQ68_002127"
     CDS             27..>436
                     /locus_tag="WGQ68_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012221570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEZ0715846.1"
                     /translation="MGKTVTLTAGHSNTDPGAVNGSDREADLAQDMRNIVASILRNDY
                     GLTVRTDGTGKGNMPLRDAVKLIRGSDVAIEFHTNAAVSKAATGIEALSTVKNKRWCQ
                     VLSKAVAKKTGWKLRGEDGFKPDNAGRHSRLAYA"
BASE COUNT          112 a          115 c          128 g           81 t
ORIGIN      
        1 caatcaatat tttttggaga tgatgaatgg gcaaaaccgt aaccttaacc gctggacaca
       61 gcaacaccga cccgggcgcg gtcaacggaa gcgaccgtga ggcggatttg gcgcaggata
      121 tgcgcaacat tgtggcttca atcctgcgta acgattacgg cttaaccgta cgcaccgacg
      181 gcacgggcaa aggcaatatg cccttgcgcg atgcggtcaa gctgattcgc ggctcggatg
      241 tggcgattga gtttcacacc aacgctgccg tcagcaaagc ggcgacaggc atcgaagcct
      301 tgagtaccgt taaaaacaaa cgctggtgtc aggtgttgag caaagccgtt gccaagaaaa
      361 ccggctggaa actgcgcggc gaagacggct ttaaacccga caatgcgggt cggcattcgc
      421 gcctggctta tgcgca
//
LOCUS       DAWFHY010000212          420 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.212, whole genome shotgun sequence.
ACCESSION   DAWFHY010000212 DAWFHY010000000
VERSION     DAWFHY010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..420
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.212"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(130..>420)
                     /locus_tag="WGQ68_002128"
     CDS             complement(130..>420)
                     /locus_tag="WGQ68_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0715847.1"
                     /translation="HIAAMQAKSNVAGTHLLNLGSGRASSVLEIIRAFEAASGLTIPY
                     EVKPRRAGDLACFYADPSYTKAQIGWQTQRDLTQMMEDSWRWVSNNPNGYDD"
BASE COUNT          115 a          100 c           97 g          108 t
ORIGIN      
        1 aggatttttc tcataaattt cagaggatag ccaaaaaata taaacagatt atagcagaca
       61 gaatgtgtgt ttttcagata aagaggccgt ctgaaaacat ctctttcaga cggcctgtat
      121 caggtcaact taatcgtcgt agccattcgg attattactc acccagcgcc atgagtcttc
      181 catcatttgg gttaaatcac gctgggtttg ccagccgatt tgcgcctttg tataggaagg
      241 gtcggcatag aagcacgcca aatcaccggc acggcgcggt ttgacttcat acggaatcgt
      301 caaacccgaa gctgcttcaa atgcgcggat gatttccaac accgaagaag cgcggccgga
      361 gcctaagttc agcaaatgcg tgcctgctac attacttttt gcctgcatag ccgcgatatg
//
LOCUS       DAWFHY010000213          415 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.213, whole genome shotgun sequence.
ACCESSION   DAWFHY010000213 DAWFHY010000000
VERSION     DAWFHY010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.213"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          116 a          102 c           92 g          105 t
ORIGIN      
        1 atagtggatt aacaaaaacc agtacggcgt tgcctcgcct tagctcaaag agaacgattc
       61 tctaaggtgc tgaagcacta agtgaatcgg ttccgtacta tttgtactgt ctgcggcttc
      121 gtcgccttgt cctgattttt gttaatccac tataaagaca gtcgggcatc tgcagccgtc
      181 attcccgcgc aggcgggaat ctagacattc aatactaagg caatttatcg ggaatgactg
      241 aaactcaaaa aactggattc ccactttcgt gggaatgacg gcagagcggc ttctgttgct
      301 cccgataaat gccgcaatct caaatcccgt cattcccgcg caggcgggaa tctagacatt
      361 caatgctaag acaatttatc gggaatgact gaaactcaaa aaactggatt cccac
//
LOCUS       DAWFHY010000214          414 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.214, whole genome shotgun sequence.
ACCESSION   DAWFHY010000214 DAWFHY010000000
VERSION     DAWFHY010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.214"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..228
                     /locus_tag="WGQ68_002129"
     CDS             <1..228
                     /locus_tag="WGQ68_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEZ0715848.1"
                     /translation="ACRNRPLTETQTKRNRYLSKTRYVVEQSFGTLHRKFRYARAAYF
                     GLIKVSAQSHLKAMCLNLLKAANRLSAPAAA"
BASE COUNT          100 a          101 c          117 g           96 t
ORIGIN      
        1 gcctgccgca accgtccgct gacggaaacg caaaccaaac gcaaccgata tttgtcgaag
       61 acccgttatg tggttgaaca aagcttcggt acgctgcacc gtaaattccg ctatgcgcgg
      121 gcagcctatt tcggactgat taaagtgagt gcgcaaagcc acctaaaggc gatgtgtttg
      181 aacctgttga aagccgccaa caggctaagt gcgcccgctg ccgcctaaaa ggcagcccgg
      241 atgcctaatt atcgggcgtc cggtgaggat taagggggta tttgggtaga attaggcggt
      301 atttggggcg aaaacaactg aaaacctgtg tttgggtttc ggctgtcggg agggaaagga
      361 attttgcaaa ggtctctttt cgtcattccc gccacttttc gtcattcccg cgaa
//
LOCUS       DAWFHY010000215          413 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.215, whole genome shotgun sequence.
ACCESSION   DAWFHY010000215 DAWFHY010000000
VERSION     DAWFHY010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.215"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>413
                     /locus_tag="WGQ68_002130"
     CDS             <1..>413
                     /locus_tag="WGQ68_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715849.1"
                     /translation="TNDTYDHLAKNIYQNLLFQTRLQPYLNQISFKMENDTFTLDFSG
                     LVQAFNHVKETNPQKAFVDLAEMLAYGELRSWYEGRRLMADYVEEAKKAGKFEDYQKV
                     LGQETVALLAKTSGTQADDILQNVGFGHNKNVSLYG"
BASE COUNT          135 a           75 c           88 g          115 t
ORIGIN      
        1 accaacgata catacgacca tctcgccaaa aacatctacc aaaacctgtt gttccaaacc
       61 cgtttgcagc catatttgaa tcaaatcagt ttcaaaatgg aaaatgatac gttcactttg
      121 gattttagtg gtcttgttca agcatttaac catgtcaaag aaactaatcc gcaaaaagct
      181 tttgtggatt tggccgagat gcttgcatat ggcgaacttc gttcttggta tgaaggccga
      241 agactaatgg ccgattatgt ggaggaggca aaaaaagcag gtaaatttga agattaccag
      301 aaagtgttgg gtcaggagac cgttgcatta ttagctaaaa catcgggtac gcaagcagat
      361 gatatcctgc aaaatgtagg ctttggtcat aataaaaatg tttctttata tgg
//
LOCUS       DAWFHY010000216          404 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.216, whole genome shotgun sequence.
ACCESSION   DAWFHY010000216 DAWFHY010000000
VERSION     DAWFHY010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.216"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          106 a           70 c          116 g          112 t
ORIGIN      
        1 caggtgcgtg ggaatgacgg gatgcaggtt cgtggggatg acgaatttca ggtttctgtt
       61 tttggttttc tgtttttgtg aaaataacgg gattttagct tgtgggtatt tactggaaaa
      121 aacagaaacc gctccgccgt cattcccgcg caggcgggaa tctagacatt caatgctaag
      181 gcaatttatt gggaatgact gaaactcaaa aaactggatt cccactttcg tgggaatgac
      241 gggatgtagg ttcgtgggaa tgacgggata taggtttccg tatggatgga ttcgtcattc
      301 ccgcgcaggc gggaatctag acattcaatg ctaaggcaat ttattgggaa tgactgaaac
      361 tcaaaaaact ggattcccac tttcgtggga atgacgggat gcag
//
LOCUS       DAWFHY010000217          402 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.217, whole genome shotgun sequence.
ACCESSION   DAWFHY010000217 DAWFHY010000000
VERSION     DAWFHY010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.217"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          112 a           72 c           99 g          119 t
ORIGIN      
        1 ctgtttttgt aggaatgatg aaattttgag ttttaggaat ttaccggaaa aaacagaaac
       61 cgctccgccg tcattcccgc gcaggcggga atctagacct tagaacaaca gcaatattca
      121 aagattatct gaaagtccga gattctggat tcccactttc gtgggaatga cgggatgtag
      181 gttcgtggga atgacgggat tttaggtttc tgattttggt tttctgtttt tgtgggaatg
      241 atgaaatttt gagttttagg aatttaccgg aaaaaacaga aaccgctctg ccgtcattcc
      301 cgcgcaggcg ggaatccaga ccttagaaca acagcaatat tcaaaggtta gctgaagctt
      361 tagagattct ggattcccac tttcgtggga atgacgaatt tt
//
LOCUS       DAWFHY010000218          399 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.218, whole genome shotgun sequence.
ACCESSION   DAWFHY010000218 DAWFHY010000000
VERSION     DAWFHY010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.218"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT           78 a           86 c          119 g          116 t
ORIGIN      
        1 agggctaatc tagtacagcc ccttagattt aattcactat acctgcgtaa tggtatcggg
       61 tactcatcat caacctgcct tgcattcggt tgtgttgtcc ggcaactgtc cggttgtgtc
      121 gatgggttgc cctgccgctc cctgtgctgc gcaacggttg tgtgccttgg gtgggcgcgg
      181 gtggcggccg ggcggtttgt tgctatgata cgggagttcc gatatacaag ggtgggcttc
      241 agcccaccaa tcaacgaccg aaaaagaaaa aacgttgggc tgaagcccac tctacgcaat
      301 tacgcaactg ttatcgtggg gatgacggga ttttaggttt ttgtttttct gtttttgcgg
      361 gaaggatggg ttttaggttg cggtcattta tcggaaaag
//
LOCUS       DAWFHY010000219          389 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.219, whole genome shotgun sequence.
ACCESSION   DAWFHY010000219 DAWFHY010000000
VERSION     DAWFHY010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.219"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..158
                     /locus_tag="WGQ68_002131"
     CDS             <1..158
                     /locus_tag="WGQ68_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014581020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEZ0715850.1"
                     /translation="VEQSFGTLHRKFRYARAAYFGLIKVSAQSHLKAMCLNLLKAANR
                     LSAPAAA"
BASE COUNT          103 a           79 c          112 g           95 t
ORIGIN      
        1 tggtcgaaca gagcttcggt acgctgcacc gtaaattccg ctacgcccgg gcagcctatt
       61 tcggactgat taaagtgagt gcgcaaagcc atctgaaggc gatgtgtttg aaccttttga
      121 aagccgccaa caggctaagt gcgcccgctg ccgcctaaaa ggtgacccgg atgcctgatt
      181 atcaggtgtc cgggcaggat taaaggggta tttgggtaaa attaggaggt atctggggcg
      241 aaaacagccg aaaacctgtg tttgggtttc ggctgtcggg agggaaagga attttgcaaa
      301 ggcctcaaga tatagtggat taaatttaaa tcagtacggc aaggcgaggc aacgctgtac
      361 tggtttaaat ttaatctact atatttccg
//
LOCUS       DAWFHY010000220          380 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.220, whole genome shotgun sequence.
ACCESSION   DAWFHY010000220 DAWFHY010000000
VERSION     DAWFHY010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.220"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>380
                     /locus_tag="WGQ68_002132"
     CDS             <1..>380
                     /locus_tag="WGQ68_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002222085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEZ0715851.1"
                     /translation="EFGRNAAQAVAAVADKLGNTQSYERYQEARTLLEAELQNTDSEA
                     EKAAIRASLGQVNAYLAENQSRYDTWKEGGIGRSILHGAAGGLTTGSLGGILAGSGTS
                     LAAPYLDKAAENLGTAGKAAVNALG"
BASE COUNT          101 a          120 c          116 g           43 t
ORIGIN      
        1 gagttcggca gaaacgccgc ccaagccgta gcggccgttg ccgacaaact cggcaatacc
       61 caaagttacg aacggtatca ggaagcccga accctgctgg aggccgaact gcaaaacacg
      121 gacagcgaag ccgaaaaagc cgccatccgc gcatccctcg gccaagtaaa cgcctacctt
      181 gccgaaaacc aaagccgcta cgacacctgg aaagaaggcg gcataggcag gagcatactg
      241 cacggggcgg caggcggact gacgaccgga agcctcggcg gcatactggc cggcagcggc
      301 acttcccttg ctgcaccgta tttggacaaa gcggcggaaa acctcggtac ggcgggcaaa
      361 gcggcggtca acgcactggg
//
LOCUS       DAWFHY010000221          350 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.221, whole genome shotgun sequence.
ACCESSION   DAWFHY010000221 DAWFHY010000000
VERSION     DAWFHY010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..350
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.221"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT           99 a           85 c           97 g           69 t
ORIGIN      
        1 gtgggaatga cgattcagaa gttgcctgaa acctaaaaaa ctgaaaccga acgagccgga
       61 tttccgcttt cgcgggaatg acggcgcata agttcccgtg cggacagacc tagattccca
      121 cctgtgtggg aatgacgatg cagaagttgc ccgaaaccaa aaaactgaag ccgaacggtc
      181 tggattcccg ctttcgcggg aatgacggcg cataagttcc cgtgcggaca gacctagatt
      241 cccacctgcg tgggaatgac gattcagaag ttgcccgaaa ctcaaaaaaa acgaaaccga
      301 acgaaccgga ttcccgcttt tacgggaatg acgggatttt gggttgtggt
//
LOCUS       DAWFHY010000222          348 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.222, whole genome shotgun sequence.
ACCESSION   DAWFHY010000222 DAWFHY010000000
VERSION     DAWFHY010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.222"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT           91 a           80 c           79 g           98 t
ORIGIN      
        1 tacggaaacc tgcatcccgt cattcccacg aaagtgggaa tccagttttt tgagtttcag
       61 tcattttcaa taaattgcct tagcattgaa tgtctagatt cccgcctgcg cgggaatgac
      121 gaatccatcc atacggaaac ctgcaccgcg tcattcccac gaaagtggga atccagtttt
      181 ttgagtttca gtcatttccg ataaattgcc ttagcattga atgtctagat tcccgcctgc
      241 gggggaatga cggattttag gttggggtca tttattggaa aaagcagaaa ccgctccgcc
      301 gtcattccca cgaaagtggg aatctagaaa tttaatgttg cggcacta
//
LOCUS       DAWFHY010000223          333 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.223, whole genome shotgun sequence.
ACCESSION   DAWFHY010000223 DAWFHY010000000
VERSION     DAWFHY010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..333
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.223"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..230
                     /locus_tag="WGQ68_002133"
     CDS             <1..230
                     /locus_tag="WGQ68_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002232671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEZ0715852.1"
                     /translation="DGWYDFRCQNVVYAPDRSPEISRMLSSILAGYAWDTENPFVAKS
                     EQRLSVLSEWVGQLETEYSELNLNRYSVASP"
BASE COUNT           83 a           90 c           81 g           79 t
ORIGIN      
        1 tggacggctg gtacgatttc cgctgccaaa acgtcgtgta cgcgcccgac cgcagcccgg
       61 aaatcagccg tatgctttct tcgattttgg caggctacgc gtgggatacc gaaaacccgt
      121 tcgtggcgaa atccgaacaa cgcctgtcgg tcttatcaga atgggtcggt cagttggaaa
      181 ccgaatatag tgaattaaat ttaaaccggt acagcgttgc ctcgccttag ctcaaagaga
      241 acgattctct aaggtgctga agcaccaagt gaatcggttc cgtactattt gagacctttg
      301 caaaattcct ttccctcccg acagccgaaa ccc
//
LOCUS       DAWFHY010000224          330 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.224, whole genome shotgun sequence.
ACCESSION   DAWFHY010000224 DAWFHY010000000
VERSION     DAWFHY010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.224"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          102 a           93 c           60 g           75 t
ORIGIN      
        1 tttcacaaaa acagaaaacc aaagaaattc gtcattcccg cgcaggcggg aatccagtgc
       61 gttgagtttc aactatttag aataaatttt gaaactctaa tcccgtcatt cccacgaaag
      121 tgggaatcca ggacgtaaaa tctgaagaaa ccgttttacc cgataagttt ccgcaccgac
      181 agacctagat tcccgcctgc gcgggaatga cgaatccatc cgtacggaaa cctgcatccc
      241 gtcattccta cgaacctaca tccatacgga aacctgcatc acgtcattcc tacgaaccta
      301 cattccgtca ttcctacgaa agtgggaatc
//
LOCUS       DAWFHY010000225          324 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.225, whole genome shotgun sequence.
ACCESSION   DAWFHY010000225 DAWFHY010000000
VERSION     DAWFHY010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.225"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          105 a           63 c           56 g          100 t
ORIGIN      
        1 tatcggtggc caaagtaccg ctgccatttc cgatctcgcc ggggaaatga aaaggctcgc
       61 taaaaaatca aaaatgttac tgaaaggact ttggtcattt ttatcctcta caatatctgc
      121 atttaaaaat aaatcagtca ttgtttaaac cttactgtaa aactgtacaa ctgctaaact
      181 gtaaacacag aaagaagcga cactaacagc acaaaacaaa agatatccaa acactactaa
      241 gtgcattttt ctgacttttt tcgttgcgtg atgtattgat gctatttgtg ctgttcttat
      301 atatgttgat gctggttacg tcgc
//
LOCUS       DAWFHY010000226          320 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.226, whole genome shotgun sequence.
ACCESSION   DAWFHY010000226 DAWFHY010000000
VERSION     DAWFHY010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.226"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            complement(30..>320)
                     /locus_tag="WGQ68_002134"
     CDS             complement(30..>320)
                     /locus_tag="WGQ68_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003714176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEZ0715853.1"
                     /translation="HVAAMKAKGGVAGVHLFNLGSGRAYSVLEIIRAFEAASGLNIPY
                     QIQPRRAGDLACSFADPSHTKQQTGWETKRGLQQMMEDSWRWVSRNPNGYGD"
BASE COUNT           66 a           81 c           90 g           83 t
ORIGIN      
        1 aggatggttt ttttgtttgc ggtttgcatt taatccccat atccgttggg gttgcggctg
       61 acccaacgcc acgaatcttc catcatttgc tgcaagccgc gtttggtttc ccagccggtt
      121 tgttgtttgg tatgggacgg gtcggcaaag gaacacgcca agtctccggc gcgtcggggt
      181 tggatttgat agggaatatt taagccggat gcggcttcaa aggcacggat gatttccaaa
      241 acggaatagg cgcgccccga acccaagttg aacaaatgta cgccggcaac accgcctttc
      301 gccttcattg ccgcgacatg
//
LOCUS       DAWFHY010000227          320 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.227, whole genome shotgun sequence.
ACCESSION   DAWFHY010000227 DAWFHY010000000
VERSION     DAWFHY010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.227"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>320
                     /locus_tag="WGQ68_002135"
     CDS             <1..>320
                     /locus_tag="WGQ68_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010980934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715854.1"
                     /translation="AAALSGDLANMLKAYSAAETKEAQLALLDNLIHKWAETDSNWGK
                     KSPMRLSTDWTQTANEGIALTPSQVAQLKKNALVSLSDKAKAAIDAARDRIAVLDAYT
                     GQDS"
BASE COUNT           93 a           80 c           75 g           72 t
ORIGIN      
        1 gctgccgcat tgtccggcga tttggccaat atgctgaaag cttattctgc cgccgaaact
       61 aaagaagcac agttggcatt gttagataat ttgattcaca aatgggcgga aaccgattcg
      121 aactggggca aaaaatcgcc aatgcgactt tcaaccgatt ggacgcaaac ggctaatgaa
      181 ggtattgcac tgacaccatc ccaagtagca caactaaaaa agaacgcttt agtttccctt
      241 tctgataaag ctaaagcagc tattgacgcc gcccgcgacc gcattgccgt gcttgatgcc
      301 tacacggggc aggattccag
//
LOCUS       DAWFHY010000228          316 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.228, whole genome shotgun sequence.
ACCESSION   DAWFHY010000228 DAWFHY010000000
VERSION     DAWFHY010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..316
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.228"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT           78 a           59 c           91 g           88 t
ORIGIN      
        1 cctgcgcggg aatgacggga ttttaggttt ctgtttttgg ttttctgttc ttgtgggaat
       61 gatgaaattt taagttttag gaatttatcg gaaaaaatag aaaccgctcc gccgtcattc
      121 ccgcgcaggc gggaatctag accttcaatg ctaaggcaat ttatcggaaa tgactgaaac
      181 tcaaaaaact ggattcccac tttcgtggga atgacggaat gcaggttcgt gggaatgacg
      241 gaatgcaggt tcgtgggaat gacgtggtgc aggtttccgt acggatggat tcgtcattcc
      301 cgcgcaggcg ggaatc
//
LOCUS       DAWFHY010000229          310 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.229, whole genome shotgun sequence.
ACCESSION   DAWFHY010000229 DAWFHY010000000
VERSION     DAWFHY010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..310
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.229"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT           85 a           56 c           75 g           94 t
ORIGIN      
        1 attttaggtt tctgattttg gttttctgtt tttgtaggaa ggatgaaatt ttgagtttta
       61 ggaatttatc ggaaaaaaca gaaaccgttc tgccgtcatt cccgcgcagg cgggaatctg
      121 gaatttcaat gcctcaggaa tttatcggaa aaaaccaaaa cccttccgcc gtcattccca
      181 cgaaagtggg aatctagaaa tgaaaagcag caggaattta tcggaaacga ccgaaactga
      241 acggactgga ttcccgcctg cgcgggaatg acgggatttt aggtttctga ttttggtttt
      301 ctgtttttga
//
LOCUS       DAWFHY010000230          301 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.230, whole genome shotgun sequence.
ACCESSION   DAWFHY010000230 DAWFHY010000000
VERSION     DAWFHY010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..301
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.230"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>301
                     /locus_tag="WGQ68_002136"
     CDS             <1..>301
                     /locus_tag="WGQ68_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEZ0715855.1"
                     /translation="NIFHNVSLYEIIFSDNGNTLTLSFTDTIEGNYFGYIKCSNILNF
                     KLDTNNFVDYEDKEDSLFPLFIPEIELYKYQFYSEIIIDVGIIIKISAETINFEPL"
BASE COUNT          113 a           35 c           41 g          112 t
ORIGIN      
        1 aaaatatttt tcacaatgta agcctttatg aaataatctt ttccgataat ggaaataccc
       61 ttacattatc ttttacagat acaattgaag gtaattattt cggatatatc aaatgcagta
      121 atattttgaa ttttaaatta gatacaaata atttcgtaga ttatgaggat aaggaagata
      181 gcttgtttcc cttgtttata cccgaaatag agctatataa ataccaattt tatagtgaaa
      241 ttattattga tgtagggatt attataaaaa tatctgctga aacaattaat tttgagccac
      301 t
//
LOCUS       DAWFHY010000231          298 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.231, whole genome shotgun sequence.
ACCESSION   DAWFHY010000231 DAWFHY010000000
VERSION     DAWFHY010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.231"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
     gene            <1..>298
                     /locus_tag="WGQ68_002137"
     CDS             <1..>298
                     /locus_tag="WGQ68_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002224013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunity 49 family protein"
                     /protein_id="HEZ0715856.1"
                     /translation="ANDTEAFVNRYDLNRHMIYNTLLMVEGKQLDRLKQRSEKVLAHP
                     TPSKWLQKRLYDYRFFLAFAEQDAEAMKAALEPLFDKKTARMAAKETLSYFDFYL"
BASE COUNT           80 a           78 c           69 g           71 t
ORIGIN      
        1 tcgccaacga tacagaagcc ttcgtaaacc gatacgacct caaccgtcat atgatttaca
       61 atactctgct gatggtggag ggtaagcagc ttgatcggtt gaaacaacgt agcgagaaag
      121 tcttggcgca tcccacccct agcaaatggc tgcaaaagcg gttgtacgat taccgcttct
      181 tcctcgcttt cgccgaacag gatgccgagg cgatgaaggc cgccttagag ccgctttttg
      241 ataaaaaaac cgcgcgtatg gctgccaaag aaacattgtc ctatttcgat ttctacct
//
LOCUS       DAWFHY010000232          298 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.232, whole genome shotgun sequence.
ACCESSION   DAWFHY010000232 DAWFHY010000000
VERSION     DAWFHY010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.232"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT          102 a           42 c           53 g          101 t
ORIGIN      
        1 tgctttgaaa tcggtattgc catctatgaa ccaccacttt gttttatttc agcgggcttg
       61 agatgtgtat aagaatattg ttttgaataa atttaaaaaa atgataatcg ttattgaaga
      121 tttttaaagg aaagcgtaga gtgccaattc tatgaagcaa tacggtaagt aacaatgaaa
      181 atatctactg cttgggtata gagcatattt cacaacccgt aactattctt gcggaaacag
      241 agaaaaaagt ttctcttcta tcttggataa atatatttac cctcagttta gttaagta
//
LOCUS       DAWFHY010000233          269 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.233, whole genome shotgun sequence.
ACCESSION   DAWFHY010000233 DAWFHY010000000
VERSION     DAWFHY010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.233"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT           77 a           62 c           59 g           71 t
ORIGIN      
        1 ggttgctgtt atagtggatg aacaaaaacc agtacggcgt tacctcgcct tagctcaaag
       61 agaacgattc tctaaggtgc tgaagcaccg agtgaatcgg ttccgtacta tccttactgt
      121 ctgcggctcg tcgccttgtc ctgatttttg ttaatccact atgtagttgg ttaaaataaa
      181 actaagccaa ggaagcactg ccgtcattcc cgcgcaggcg ggaatccaaa ccttagaaca
      241 acagcaatat tcaaagatta tctgaaagt
//
LOCUS       DAWFHY010000234          259 bp    DNA     linear   BCT 15-MAR-2024
DEFINITION  TPA_asm: Neisseria meningitidis strain S181
            SAMN37720367-rid20437593.denovo.234, whole genome shotgun sequence.
ACCESSION   DAWFHY010000234 DAWFHY010000000
VERSION     DAWFHY010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37720367
            Sequence Read Archive: SRR26318897
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Neisseria meningitidis
  ORGANISM  Neisseria meningitidis
            Bacteria; Pseudomonadota; Betaproteobacteria; Neisseriales;
            Neisseriaceae; Neisseria.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (28-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 28-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001985371.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001985371.1
            Genome Coverage       :: 240x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/29/2023 05:24:15
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,081
            Genes (coding)                    :: 1,958
            CDSs (with protein)               :: 1,958
            Genes (RNA)                       :: 56
            rRNAs                             :: 1, 1, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1, 1 (5S, 16S, 23S)
            tRNAs                             :: 49
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 123
            CDSs (without protein)            :: 123
            Pseudo Genes (ambiguous residues) :: 0 of 123
            Pseudo Genes (frameshifted)       :: 47 of 123
            Pseudo Genes (incomplete)         :: 76 of 123
            Pseudo Genes (internal stop)      :: 19 of 123
            Pseudo Genes (multiple problems)  :: 18 of 123
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Neisseria meningitidis"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37720367-rid20437593.denovo.234"
                     /strain="S181"
                     /isolation_source="swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:487"
                     /country="China: Jiangsu"
                     /lat_lon="31.27 N 120.73 E"
                     /collection_date="2018"
                     /collected_by="Suzhou CDC"
BASE COUNT           70 a           71 c           53 g           65 t
ORIGIN      
        1 gggcggtcgg atgccgcctg aaactccgtc attcttgcaa cttttcgtca ttcccgtgaa
       61 aacgggaatc tagaacttca aactttcaga taatctttga atattgccgt tgcccgacgg
      121 tttggattcc cgcttgcgcg ggaatgacgg tagagcggtt tcagttgctc ccgataaatg
      181 ccgcaatctc aaatcccgtc attccctcaa aaacagaaaa acaaaatcag aaacctaaaa
      241 tcccgtcatt cccgcagct
//
